LOCUS       DAWDWH010000001        44549 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_435_length_44549_cov_14.377849, whole genome shotgun sequence.
ACCESSION   DAWDWH010000001 DAWDWH010000000
VERSION     DAWDWH010000001.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 44549)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 44549)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..44549
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_435_length_44549_cov_14.377849"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4675..4684
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20610..20619
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    24687..24696
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    42465..42474
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    43567..43576
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        13753 a         8032 c        11321 g        11393 t
ORIGIN      
        1 acgtgatgca actatatttg cggctcagac aggattaaag atggtgaaaa tgagagaaag
       61 aagaaataaa gagcaggtta acagatagaa ttttgaattc aaaaatatct tttagagata
      121 tctttgcgtg gaaaaggagt gacatcaaat gatagatata tatacagata aaaaagaatc
      181 aaaggactgg attcttcaaa acgacttgta tttcaatttg aaacaaaata tggattaggt
      241 acaatccgta atttgtcatc cggatgcaag acattactta atattgtaaa gcatcctgat
      301 aaggtggtga atgtagaaga atgtggtccg aatgtgcttg aaattatttt tactctggat
      361 aatataaaga tttatatgtc cagaccaacg ctttttgata tcccggatga tgtgcaaatc
      421 agatttaatg attctgatat agtgaccgga gggagaggat ataatgcctg gtggggcaag
      481 gagtatgaaa ggagagaagc agatgattta taaaaatatt acatttaaag ctgatccatt
      541 ttcatacaat ctggaatttg atgacagaat aactcttgtt ggtggagata gtggtaccgg
      601 taaaactgtg ctttatgaaa tgttagaaga tttacgatta actgatgaat ataaagctat
      661 aaaattgttt aattacaaat cagatcattt ctcagagtcg attgaaaagt gcagagatag
      721 ttttattgtg gtcgataatg ctgataattt gatgaatgat gaggtcagaa gttttattaa
      781 ttttgagccg tcgaatcaat atatgctttt cctacgaaat tgcgatggtc tcaatgtttc
      841 taataggagt tttaaggtgc tgaagtttga caataatagg ataacgctgg aagaggagct
      901 atgatatgaa atatttatgg acagaagaca ctggtgctgg actccatttc tggaagttgg
      961 tcaatcagct tttctttgat gatgcccttg tggtcgaaag taaagaaagt aatcagggct
     1021 tattggatgc gttggcggat atagacatga aagaggacga taagtattat atcgcgtttg
     1081 attatgtagt tgataatcag gatattcgta acaagtatcg tatgttgaaa tcaatagcag
     1141 ataaggcaga aggaaagatc ataattctgg atatgatttg ttttgagtac ctgattcttg
     1201 tttttgataa gctggtggca tggacgggta ccggaaagac agataaaatc aagattcgtg
     1261 aagaggtatt ggctacagtt gaggaccaca gaattaattt gtcaaagatt gatgacgaga
     1321 agactttgca gtatatagca ggttttaaga gatattctac agaacgtgtg atgaagtcgt
     1381 tagtaggtga atttacacaa aacgaaaagt ggtctgttaa aggagtgcat atgggggaat
     1441 gttggtataa ggattgctgt gtgtcggaac atccggataa tctgaggtgt ggtaagccgg
     1501 aagttgagga tggaagtgag aagatgcgga tgctgatgca gtcgtaggaa gtacataaaa
     1561 ttatatcgat tattatagga taaaagaaag aggtagtatg agagcagaag atgatcttac
     1621 atatcaggaa tataaagaag gcgttgaaga tgctatgcgt tatatcaaaa atcatggctg
     1681 gacaccgaga caggttaccg acagaatgac ggatgaagat aatgagctgc tcatcggcac
     1741 gtcggaagca ctctggatca tttctatcgg agcttatgaa gtggagcatg acattctgga
     1801 ggagagagta ctggagcagt tgtcgtatca tattccacgc tatgagatgg gaaaatacaa
     1861 cgacaccacg ccggaagagc gggagcttct ggaaaaggat atcgcattga ttcgatcaaa
     1921 ggtggaactg tggaaactga agagttatga ggattaaaga gtgcaatatt gaagagatta
     1981 tgttggaaga gtatagagac ttttcggaaa ggtcatttag aaaaaaataa gtacagaagg
     2041 gaagttgagc aagtagacgg tagatgatat acttaaaaca ataacataaa aatacaaagg
     2101 ggaagaatga gtttgaagaa gataaaaaaa gcactgacaa tggttttgac tgcatctgtt
     2161 gcgttatctg ctgctggatg tggaaaagat gtagcgaaaa ataatatgac agaggaaaag
     2221 actgttgaaa caacagttca ggaaagctca agtgaaacta tttcggatga tacaaaaacg
     2281 acagagacag cacagtatgc gatggagaga agcgaaacgg agagtacaaa agaaagtgaa
     2341 agtattcagg atatcacaga aacatatgaa gatatgtcgg aatatgcaat tgttgaacct
     2401 gaaacagaag gggatttgac ttcaaagcag aaaaatgctg ttaacatgtt aaattatata
     2461 atgacgataa ctcaggaaat caacaattcg aaagaaagta gactttattt ggaatcagca
     2521 tattcttcat tgattaacaa cagtgatcct gggcttatag atacaagaac ccaagagaga
     2581 attactaata ttttagatac acttgaacaa tatcgaatga ttgctgaaaa gagagagagg
     2641 ttacgatata tttatgaaca aaatcgtgca caggcacttc gtcaggcaat accagatcct
     2701 gttggattgc ttagtgcagt ttcatctgga aatattttaa agtcagcagc gtctgttatt
     2761 tatatggctg ttgatgcagc atcaagctat caaaatgctg ttacacagac agatttgcaa
     2821 tatcttcagg atggatggca attggaggat gaagaagcac aggaattgca taacagcaga
     2881 aaaagtacat ttgagtatat ggtggatatg gttcgagaga attctctgcc gggagaatat
     2941 gcgttaaatg aaaatgctgt gaatgatttt gttgagtgga aagataagac aaatcttacc
     3001 agtaaaattc aatggcttga atcaaatgag agtacatacg agaaatttgg accattttgg
     3061 ctggaactgg cgaaaagcta ttataaatca gaagagtatg ataagtgtct ggatgcgatt
     3121 aaaaagtatg aagggatttc tgtcaagatt tttcgaaagg attacgacta tgctgaagct
     3181 cttccaatgg cgattgtttc tgcaaaggag attagggata aaaatgtata tattcaaaca
     3241 gcaagagagt atagtgaggt gattctttcc aatgcagatg gtgaaaattg ggcattgcgg
     3301 tactttgtag ctcagatata tcttgatctg tataaacaaa cgggcagtaa agaatatctg
     3361 caaaaagcgt atgacattgc actgaataat gtaaatgttt tagttgagtc tcaaaaagaa
     3421 ttgaacttag catacttatc agatattcag gaggtttctg taaataaaga agcagaagat
     3481 gaagacagag aaaagaagga agttaaacaa tataataaac tcttaaaggc acagagaaag
     3541 gtcgaattgc ctccggttga tgaagcttta tatttgaact gtgatttatt gtttgcatta
     3601 gcagaacaac tggatatttc agtggatgaa caaaagaaaa tagatgctat tttgcatgaa
     3661 aatggggaac ctgtttttct tacacaggca ttggatgaaa agttttggtt cgagtcagct
     3721 tataaaaaac tagatgttca aaatattgat attgactttg atggaaagac attgaaaatt
     3781 ccggcagtgt gtatagcaga tcgatcgcgg ataacagtaa gtgttgacgg ttcttctgga
     3841 aatcaaacct ttcaggattg gacagtaaag aaagtttata gaccgaaagg agcagattgt
     3901 tctgatttta tagttatatt taccagcgaa acagctaaaa aatataaata tgaatcagga
     3961 gataaaattg cggtgcagat tactccggtt gcagagtcag cagatgaaat tctagagtat
     4021 gattataagg cgactgtaat acagaaagca tttgcattta aaacaatctc ttttgagagg
     4081 gctgcaaaat gaaaaaacga atcatggctg tagtcttatc tatagcaata ttattttctg
     4141 attcggcatc tgtttttgca tgtgacagaa agcagacaga gacctatgtt tctcagatgt
     4201 tgtttggtga taaagcggaa aaatataagt ctaatgagaa agtacagatg ttgatgaaag
     4261 cactttatct ttgctcagaa caatatgacg acaatgatac aaaagaatta gacgccttaa
     4321 aaaagaaaaa ggtaagtggt ctgccaaaat cagaagaaat tgagatcagt caaaaagaac
     4381 tgctcgaatg ctcccataat acctgggaat ataaatacaa tgctaaggaa aaacaacaga
     4441 gatccagaaa aacaattctt cgaaatacag tcagcaaggt atttggtgtt ggattttgga
     4501 atcgttattt caaaagaaac aaggaaaaat atgagagttt tgcggctgta ttatattata
     4561 tgcatattct ggcggattat ctggcggatg atccggatgc gacagaaata acatggaaaa
     4621 gcggatttgt ttcagcatat gatggaaaag cgggtgttga gataaatggt ggaannnnnn
     4681 nnnntggaat ccctcagttt acccaaaagg aaaaaaatag aaaagattat tttcttacat
     4741 tcagtaagct ggattcatcc ggacgagcag gaacagcttt tgcaaatatt tcaaaagatc
     4801 atatgccgga agcgggatca agacagcata taggaaacat aaaaccatct ggatggaatc
     4861 aaaaaaagta tgatggtatt gtcagcagca atccgggata tgtatataat cgctgtcatt
     4921 tgatagcaca tcagcttgta ggtgaagata taaaagaaaa tctgatcact ggaacacgat
     4981 atttaaatga agcgatgata ccaacagaaa atgaagtagc agattatgtt caaaaaacgg
     5041 gaaaccatgt attatacaga gtgacaccgg tttataaagg agaaaattta ctggcatctg
     5101 ggattcaaat agaggcatat tcagtagagg ataaagggaa aggaatttcg gaaaacaggt
     5161 atttttataa tgtacagcct gggatagaaa taaattacaa cagtggggaa aattcatgtg
     5221 ctgatactac atttcagaaa gaaaagatac taaagtttgt taccgttgat tcaggtgaaa
     5281 aagatcttgt acatgaaatg aataaacaac tggacatact ttttaaggat cagaaaaagt
     5341 cggaaaaaac gaatgactat aacagaatga tgagtgcgat agatgaaaat atcagaaaag
     5401 tgagaaatgt tggaaacaaa ggagaaacag cagggaagca atatattttg cgtcgtgagt
     5461 atgaatatga atattataaa atattgagga aatatttacc ttctttattg gaacaagaac
     5521 agttttttag caacgttttt agagattaaa cagcatttgt ttatctgtga aaaagtaata
     5581 aaaagagagg aaaaatgtaa ataatatgcg agcagaagat gatcttacat atcaggaata
     5641 taaagaaaac gttgaagatg ctatgcgtta tatcaaaaat catggctgga caccgagaca
     5701 ggttaccgac agaatgacgg atgaagataa tgagctgctc atcggcacgt cggaagcact
     5761 ctggatcatt tctatcggag cttatgaagt ggagcatgac attctggagg agagagtact
     5821 ggagcagttg tcgtatcata tcccacgcta tgagatggga aaatacaacg acatcacgcc
     5881 ggaagagcgg gagcttctgg aaaaggatat cgcatttatc cgatcaaagg tggaactgtg
     5941 gaaactgaaa aattatgagg attaaagagc agtaattatt gaagaaatta aaatggaatt
     6001 ttcgggagaa ctgagaaagg aagtagaaaa gcgaagctgg caaaactgaa tgagatgttt
     6061 ctggagaaag tttaatcaga catactctaa aatccctctg catacattgt aaaaagatgc
     6121 atgcagaggg atttttttga aggattatat tgaagagcgg gcagtggaag ttgcgtatta
     6181 tataattgaa acgagggcta cggtgcgtca ggcagcaaag aaattcggga tcagcaagag
     6241 tacggtacat aaggactgca cggaacgttt acggcagatt gatgcggggc ttgcggctca
     6301 ggcacgagag gttcttgatg tgaacaaatc agagcgtcat atccgcggcg gtgtagcaac
     6361 cagagaaaag tacaggcatt tgcatgagct gcaggagcat cacgcatgaa aaatataaaa
     6421 agtaaaattt cgaatggaaa tatgagattt catctcaaat aatcatatga aaaatgaaaa
     6481 aatgcgatat aatagaaaaa ataaacgaga aaacgaaaga agaaagaggg catgattttg
     6541 caggaatatc agacagttac tcatcaattt gaaccggttt ttgatgagga ttcgaaggtg
     6601 ctgatcctgg ggacatttcc gtcagtgaag tccagggagc agaattttta ttacggacat
     6661 ccgcagaacc gattctggaa ggtgcttgcg aatctgacgg gatgtgaggt tccggggaca
     6721 gtggaggaga agaaaggttt tttgttaaga gaacatatcg cggtgtggga tgtgatcgcg
     6781 aagtgtgata tcatcggttc gagtgacagt tcgatccgaa atgtagttcc ggcagatctg
     6841 tcaaggattt tagatcatgc accgatccgg cagatttatg caaatggcgg gaaggcttat
     6901 gaattgtatc agaaatatgc atatccgatc acgggaaggg agatcgtaaa actgccatcg
     6961 acaagtccgg cgaatgcggc atggcagatg gaacgtcttc tggaagcatg ggcggcagtg
     7021 aaagagaatc tgggataaac ggattctctt ttttttcttt ttttgtttca ttgtttaacc
     7081 aaatcaagta agtcccctgc ttcaattgcg aaaagcaata agcagtgggt ggggacttgc
     7141 ttgtatcagg aggagtgcaa gctccttctg ataaactgcg gagcagttat ttggttatga
     7201 gcaagtagca aagcggattg cgaatatccg cttgactgta aaacaataaa aagtataaat
     7261 ccggataatg tgaaaaaaaa gataattttt taaaagaata tgaaaaaatt tcttgcaaaa
     7321 ttgtattaaa ttttgtgaaa acagaaagca aatttatgtt atactaatta gcaaataaga
     7381 tggaatataa atgaaaaaga gtgtgatacc agagaaaaag aaacgaaatg ccggaagcaa
     7441 aaaaatgtaa tatatgacag gggaatggcg gaggcatttt tttaactgaa ggtgagaaaa
     7501 aaagtgaaca ggagagaaaa agaaccaata gattttgtaa ttacttgggt agatggaaat
     7561 gacccaaaat ggcaggaaga aaaggataag acgctgattg ctcagggact gggcggtcat
     7621 atagatggca ggaaggagcg ttaccgggac tgggacaact tgcagtactg gttccggggc
     7681 gttgagaagt atgcaccgtg ggtgaggaag atccattttg ttacctgggg acatgtaccg
     7741 gaatggctga atgtggatca cccgaagctg catatcgtaa agcatgagga ttttgttccg
     7801 aaggagtttc tgccgacgtt taattcgcat acgatcgagt ggaattttca caggatcgag
     7861 ggactgtcgg agcattttct gtattttaat gatgatatgt ttttgttaaa taaagtgaaa
     7921 ccgacgcact ttttcaaaca cgggaaaccg gtggatatgc tggctctgca gccggtggtg
     7981 gcgaataagg ataatgaagt gatgtcctac atttatttaa ataatgcgat actgctggca
     8041 aagcattttg ataagtatga aaatatgaaa aagcagccgg gagcttattt ccatgtgggc
     8101 tacccgctga agtattactg ctataatgtg atggagatgg cttttccgag gtttacgggc
     8161 ttttatacgg tacatggtcc gtcgccgctt aaaaagagca cgtatgagat gctgtggcag
     8221 gcggagccgg agcttctgag gaaggtgtgc agccatccgt ttcgtcacaa ggaggatgtc
     8281 agccagtatg tgatccggga atggcagaag ctcagcggtg attttgtgaa taaaaacagc
     8341 tactggctgt gcaaatattt tgatgtgtca gatcacaatg aaaagctgct cagaatgatc
     8401 agaaagcaca caggaaaaat tgcctgtgtc aatgacagca atcacggcat tgattttgaa
     8461 aaggcgaaag cggagatcaa tgcagcattt gcggagggat ttccggagag gtctgcattt
     8521 gagtgtgaat agaaaaaggc gaaagcggaa cgcaaatctt tgatttacaa ctaactgaaa
     8581 aaggggaaat gtatgaatat tctgttggta ggcggcactt gcagcctgat gaataatatc
     8641 attctcaagc ttcgaaaaga agggcacaga gtttttctgc tgacgggaga taaatataaa
     8701 cacaacaaat atgaaaaagt atttgaacgg tacgattttc cgtatgacag tgagaatctt
     8761 aatgagatca tcgagagtgt caatccggat gtaacgatcc tgctgggagc ttttgatacg
     8821 aactatctct ggaatggcga ggagcgagag acggttcatt ttatttcgca tatggtgaat
     8881 atcctggtgg cctattcggt ggtgaaccga ggcaaactga tttatctgtc ttcggatgag
     8941 gttttcagtg gcgattatcc ggatgatatc acagaaggag agaaattttc cggatatgga
     9001 atacgcccgg cagcgttgtc acaggcagag gagctgtgtg agaatttccg ggtgaaccgg
     9061 ggactggata tcacggttct gcggctggat catctgtaca gcattccgaa ggataaaaat
     9121 gatatcaaca atatctgtgc gggcatgtgt ctggagtatc tgcgtgacgg atatatcaaa
     9181 gcggatgaga accacacctt ttcgatgttg tacgagaaag atgccgtgga atatatttac
     9241 aaggtagtaa aaagtaagaa aagggcacat tcgatctatc atctgtcatc caatgatgtg
     9301 gtaaatgagg tagagctggc atcctggatc ttgcaggtga tgcaaagtga agcaaacatc
     9361 gtgacgacac cggggaaaaa cggaagatgt gtgctttccg gaagaaaatt tgaagaagaa
     9421 tttgatgttt acacattctg cgactgcaaa aagatcattg aaaagatggc tctttatatg
     9481 cagaagcaca aggatgtatt tgtaaatgaa gataaagagg gatccatcct gaaaaacctc
     9541 tgggagaaat ggaaatggct ggtgcgggca ctggtaccgt ttattgagaa cctgatctgt
     9601 tttattccgt ttttcatgct caataaccga acggtcggca gtcaatattt tgcaaatctg
     9661 gacccgtttc tgctctatgt gctgctgttt gcaatcgtgt acgggcagca gcaggcaacg
     9721 ttctcggcaa tctgtgcggt agccggatat atgttccggc agatgtatac gaggaccggt
     9781 tttgaggtac ttttagatta taatacttat gtctggatcg cacagctgtt tattctggga
     9841 ctggttgtgg gctatatgag agatcagatc cgtacgatgc gggcggagag tgcggagctg
     9901 gaggagcatt tatcaaggca gatcgtggat atccgtgata tcaacgagag caacgtgcgt
     9961 gtgcgtgagg tcatggagca gcagctggtc ggccagaaag acagtcttgg aaagatttac
    10021 agtatcacaa gacagctgga tcagagtatg ccggatgagg ttgtcttcca tgcggttgaa
    10081 atgctcacaa aactgatgga aacaaaagat gtggcatttt acagtgtggt gaatgatgat
    10141 tatgcacgtc tcttttcggc atcttcggaa aaggcgagaa gtcttggaaa ctccatccgc
    10201 taccgtgaaa tgacggaaat ctatgatgaa cttctggaag aaaaggtgta tatcaataaa
    10261 acgatggatg aacgttatcc gcttatggta cggggcattt atgaggatgg aaaactgcag
    10321 atggtcatta tgctctgggg cttaaactgg gagaagatga cacttggacg ggcaaacttc
    10381 ctgacggttg tcagctacct gatccagaat gccgtgctgc gggcacagag atatatacag
    10441 gccctggaag aaaagcgtta cagctatggc tcccgtattc tggaaaaaga tgcgtttgag
    10501 tctctggttc aggcctatgt gaaagcagaa gaaaagaacc tggtagaatg tacgctgctg
    10561 aaagtctgtg tacagccgga acagtatcag gaggttggcc agaaaatggt tgaaaagatg
    10621 cgagacagtg attacatcgg tgttatggaa gatgctcagc tttatgtgct gcttgctaat
    10681 accggtcagg aaaatgccgt gatcgttcag aagcgatttg aggagaatgg atatcgtaca
    10741 gaaattgtgg agtagataaa gtatgtcccg gagaatgaaa ttttttatat ttttacttgt
    10801 gataaacttt attgtggttg tgatctatgt gatctggaac catttgagag gaaaagaaaa
    10861 aaatctcagt acatggatga aggcgatcgt gatgctttta tgcccggtgg tcggaccgac
    10921 gtttctctgt gcggcctttt tgctgtacaa gctgtttatg gcacaggcga tggatctttc
    10981 agatgttgtt ttcagcaaag agagagtcaa aacctttatt cctccggatg aagagcgggg
    11041 aagaaatatg gtttccatcg aagaagctct ggaggttacg gataaaaaga gccttcgtac
    11101 atttatgatg aatgtgatcc ggggagatta ccgaaaatat ttgtcttcga tctcactggc
    11161 tttgaataat gaggatacgg agacagcaca ttatgcagct tcgatccttc aggatgtact
    11221 tggagaattt cgagaaaacg tacaggagaa gtatcagtta agccaggtgg aggatgaaca
    11281 gcaggtgcag cactgcattg agctgatcga gtatatgaat ccgatcttgg aacagaaaat
    11341 tctgacaaat ctggaacaaa attccatgat tgagcgaatg gatgaggtac ttgagaaggc
    11401 ctggagtctg gaaccggaaa agatcagcag tgtggtctac gaaaaactgt gtgcaaggtt
    11461 gatggagatt ccagattatg aaaaatgcgg caagtgggca cagcgttcca tggagcagta
    11521 tccaggagtg ctctcgagct atacctgcca gctgaaactg ttcttttcca acggggataa
    11581 ggagaatttt ttccgtgtca tgaacgagct gaaggcttcg gacatcacga ttgataaaga
    11641 aacactggaa ctgatccgaa tatttatgta atgcagaagg atgaaatgat atgtcaatga
    11701 caacactggt aattttaaga tttctgggca tttttgcagc ctatatgggt gtgacactgg
    11761 cacttccggc actgatgttc cgcaggatcc tgcggggcag aagtctggca gaacagtttc
    11821 tgatgtgtta caccttcggt aatttttata ttatcaatat tgtgttctta ctgcagctgt
    11881 tgcatatttc aaatttcctt acgcttgccg ggctgactgc ggtattgagt attgtaatcg
    11941 gaggcagagt caatcggatt cctttaaagc agcaggcggg aaatacctgg catttgtttg
    12001 gcaaacttct gagaggaaga atgaaattaa aatcagcaat ttttcttttc cttggaaaat
    12061 gtgcagaggg aataaagaga ctggcgaaat ttttctatcg tcatattgtg aaaaatccga
    12121 tccagagtat gcttctgctg ggaatcggag tgtgtctgtg ctggatctac ggaagacaga
    12181 ttatcttagt ctatggttat cgtgcatcgg atatcccggt gcatatgagc tggatcaatg
    12241 agatgagcag aggaaagatt tttgcgaaag gtgtctatcc gtttggtttt cactgcataa
    12301 tctattatct gcatgcagtc tttcgctttg atacgtatgt gatcttatgc cagttctttt
    12361 ttgctcaggt aatctttatg catctggtat tgctggcgat gatgaagcag ctctgcaaaa
    12421 caaaatacat tccatacatc ggaacttttg tatttctgct tggaaatttc tggtccggcc
    12481 agacttactc acgattttat gcgactctgc cgcaggaatt tggtatgatt tttgtgatcc
    12541 cttccattta tttcctgatc cggttttttc agataccgaa gcagaaactg gcgaataaag
    12601 agacgaggct tacgctgcag tgctttgcga tgacgttttc tctgacactg gcgatccatt
    12661 tttacggcac gatgatcgcc ggactttgct gtatcgggat cgcgtgcgga ttttgttttc
    12721 gctttttgcg taaagaatat tttcgccgta tcatgtttac gggaatctgc agcgtttttc
    12781 tggcggtgct gccgatgggg attgcttttg cgaccggaac gccgcttcag ggttccctcg
    12841 gatggggctt aagtgtcatc aatggaggca aaagttcttc ttcgacagag acagaggcag
    12901 acgaagcaga aacattggaa gtctccaccg gagatgacaa aaatacggtg cgggttgtga
    12961 agccggatgg tacggttatg gagattgatg tcagtgatct gccgtctgca caggagaacg
    13021 aaagcggcgg acagacgcag acagaaacca cggcaccggc cgtgccaaaa gtatcttttg
    13081 gcgaaaaaat tcgaaaaatt ccggggaaag caaaaaatgc tttatcggaa atgagcagtc
    13141 gaatcctgga attcattatt aaactggatg tgaaaaacat tggttatatg atcctggcgt
    13201 cttttgcact gttgcttttg ctcggactgg tcttctgtat tttccggcag ccaggatatg
    13261 gagcaatgct gatgtccatg ggattctgta tgtggatcgt gaccattttg ctgtgtgcag
    13321 gcgtgtttgg actgccgccg ttgatggatg gagcaagatg cagtatttat tatgtttatc
    13381 tgctcagtgc ggctcttacg gcactggcag acggactgct gtatatggtg ctgccgttgc
    13441 ggaaactgcg gctggtaagg aatgctgtgt cgctggctgt tgcggcagcg gttctgatgg
    13501 gaatgttcca gaatcatatg atcaaacagt ccgatttctc cagcggtttt gtgatgaatg
    13561 gagtgatcac ctgcctgagc aatattatcc atgaaaatga agataagacc tggacgatcg
    13621 tatcggcgaa cgatgagacg cagatgggag tggatcacgg atggcattat gagacgatta
    13681 cgtttctgcg tggaatggaa actctggaaa aaaatacgaa agtcatcatt ccgacgaaaa
    13741 cggtttattt ctttattgaa aaaatacctt tggattacag cgttacttat gataagagcg
    13801 gtcagtcgat ctccagaaag ggagccagcc gttccctgcc gaatgtgggc ggcatcggta
    13861 tgtatcaggg agaaggaaga tggatcgtga tgtccagaat gtattattgg gcacaggcgt
    13921 ttatggagct ttatccgaat gaaatgaagg tctattatga ggacaataag ttcatctgct
    13981 ataagatcga gcagaatatg tatcatcagt atgattttgc gatagactac agatacaatc
    14041 agaacaagat gcaggatgag acagccgaag atacacagga tgagacgcag caacagagtg
    14101 aagcaacgaa tgaaacgcag caacagagcg atgcatccgg aaaacaggag gcaggaaaat
    14161 agagtatggt ttcaaaaaga aaatttttca gtattgtgat tatgatggtt gtactgttat
    14221 tcctgactca gttttccatg gtactgcgag atcaggcaaa ccagtatgat gccaatgcca
    14281 gtctgtctga gcgaaaagca gacggaaaag aggtcatcga acagtcagaa gcagaaagaa
    14341 aggatctgtc tttactcatt tcggaaaaaa gcgtgctttt tatcggcgat gtttccggtg
    14401 ctatgggaac ggaggtaaag agatgggcgg actatgtgaa gtgggatatg ttttccgtta
    14461 aatccatgga cgaatacatt caaagcaagg ctcatcttcc ggaaatgatc attctggaat
    14521 cagaaaagta tgcacaggga gaagatctgg attgcctgga gcgttttcag aaacggggag
    14581 cgatcattgt cttcggatgt ctggaagatc cgactctgat cgaaaataat gaggaactga
    14641 tgcactttct cggaatcagt aaggttgttt cagaaaaagt aaagcttaat ggtgtaaagt
    14701 tatttgaagg actgcttctt ggtggtgaac agatctatga aacgccggtt aaagatgacg
    14761 agaaagacag gcaggatctg acgctcgatg tgccgtggta tcaggttgga agcggcacca
    14821 agacctatat ggtcggaatg tttaaggaag aaaccagcag ggatatcaaa aatgaagatc
    14881 tgccgacgct gatctggaga aatggtatgt accggggcag tatttttgca gtggtcggcg
    14941 attatttaaa agatggcacg gccagcggga ttctggatgg aatgctgaca gaggcatcag
    15001 agtacagcat ttatccgatc gtaaatgcac agaatctgtc gatagtcaat ttcccgggat
    15061 ttgcggatga aaataatgcg gaaatgatgg agctgtacag tcagtcggtg accggagtcg
    15121 gacgtgatgt tatctggccg accctgattt ccattgtgga acacagcgac ctgaagatga
    15181 cctgctttat ccagccccag gcagattata aagacgggat tgagccggac agtgagttcc
    15241 tggagtttta tctgaaacag ttcaaggagc aaagtgccga agcaggtctt tccatggaat
    15301 ataaaagtgc agattcgctg gaggaaaaag tggatcgtga cggcgaattt tttgcaaatg
    15361 cagacagtga ttatcgctat ggtgcagcat ttgcggaaga acgggatctt accgatgtgc
    15421 tgaatttatc ggagataaag ctgctgaaaa atgtcagtac gctgatctgt gagtataccg
    15481 agaagcatcc ggtcgtgtcc tatgcttcgg actcgatcac actgcagtcg gtagtcggtg
    15541 acggcatgca ttatacatac agtgcggatc ttcgcatgcg aagcattcaa agtgcactgg
    15601 ggtatacgaa tatcatgctg aatctgcagg atattttctg gccgcagaac gaaaaagacc
    15661 gctgggagat catggagagg cgatttgcga gcaatcttct gacctactgg aataaattta
    15721 actgttttac ggcgacgaca ctttccgaga gcgaccggag ggtgcgtacc ttcctgaatg
    15781 tggattatac ggaatcgcgg gaagaaaatg tgatcacttt aaatacgaca aaagcggaca
    15841 gctggtttat tttgcggacg cacggagaaa agatcgagga catcgaaggc ggaagtcaga
    15901 caaagattga aaaagatgct tatctgatcc atgcggaaga tcataccgta aggatacagg
    15961 ttgacgaacc ggggctttcc tatgattccc ggcatcagtg ataaagaaga ggaagaacat
    16021 gaaggtttgt attgtagcag agggctgtta tccttatgtg gtcggaggcg tttccggctg
    16081 gatcaacagc atgatcaagt catttccaaa tattgaattt attctgctga cgatcgtggc
    16141 aaacagatcc gtgcgaggca agtttgtgta tgaactgccg gagaatctga cgcaggtcta
    16201 tgaactgtat ctggaggact gcgagtggga agaaggagag agagagcgaa aagtacgaaa
    16261 gcgtttccat ctctcgaaga aggaacgcac cgagctgacg aatctggtta tgaaccggaa
    16321 aatcgaatgg ggtgtgatct ttgatctgtt ccagcgaaaa ggcgtgtcgg tggatgatct
    16381 tctgatggga ccggatttct ttcagattgt ccgggagtgt tataagagac agtatgccaa
    16441 tatcgttttt tcggatttcc tgtggacgat gcgatcaatc tatctgccgc ttttctggac
    16501 attgaagatg aaagtgccgc aggcagatct ctatcactgc gtggcaaccg gatatgctgg
    16561 tgtgctcggc agtatggcaa agcatttcca taacagttcg cttttgatct ctgagcatgg
    16621 catttacacc agagagcgag aggaggagct gatcaaggca aactgggtca aaggtgtcta
    16681 caaaaacatc tggatcgagc agttcaaaaa gatgtcgctt ctggcttatc agaaagcaga
    16741 tacggtaaca tgcctctatg aacgtgcaaa acttctgcag attgatcttg gctgtccgcc
    16801 ggagaagatc cgggtgacac caaacgggat caatatggcg aaccttacag atattccggg
    16861 aaaaaccagg gaggatgagc agtggatcaa tgccggagca gtgctgcgtg tcgcaccgat
    16921 caaggatgtc aagacgatga tccaggcgtt tgcgtttgcg aaagagaaag ttccgaatct
    16981 gaaactgtgg atcatgggac caacggatga agacaaaaaa tatgcacagg aatgttttga
    17041 tttggtaaag gctctgaatg tttcggatgt ggaatttacg ggcaggatca atgtccggga
    17101 ttatctgggg aaaatggatt ttacgctgct taccagtatc agtgaaggtc agccactgac
    17161 cattctggag agttttgcgg cacacaagcc ggtcgttgca acggatgtcg gaaactgtcg
    17221 ggaactgctt ttcggagtga atgacggatt cggagattcc ggaatcctga cacatatcat
    17281 gaacattgag gagatcgcgg aggcgatggt gacgctcgcc tggcacaaag aactgcggga
    17341 gcagatggga gaggccggat acaaacgagt aaaacagttt taccggattg agcagatgaa
    17401 agaggtttac cggaatattt acaaggagtt tgcagatcag cagcagatcg aatggacaga
    17461 gaagcctttt gagatcagat agaaaaagaa gggaaagaag ctatggctgg tataggtgtt
    17521 aaattaaata acatatatgg aaaaaatacc ctgacaaccg acgtaatcgg tatgggctac
    17581 agcaccgtca tgaccatcgc tccgatgctg acggtcatcg gtgcactgtg tatcatggaa
    17641 tatttcctcg atttttcttc ggtcggatat gtgacaaggg agctgttttc ctgtacggtg
    17701 ctttatatct ttatctttgc actgctgacg gcatcgcctt ttaactcggt gctgtcaaag
    17761 tatatgtcgg acgtgatcta tgaggaaacg tatgcggaca ttatggcatg ctattatgtc
    17821 ggactgtttt taaatattct tttgagttct ctgctcggaa ttccgttttg tattcatgaa
    17881 tatcttgtcg gaaaagtgga tatcatttat gtttttaccg gatattgtgg atttatcgca
    17941 ctggtatttg tattttattc gatgctttat ctgtccatct gtaaggatta caagaagatt
    18001 tcgttcttct ttatcattgg aatgaccgtc acggtgttat tgtccttctg gtttgtgcgt
    18061 gggttacata aaagcatcac ctacagtatg cttctggcac tgacgatcgg attctggctg
    18121 atcgcggctt tggagttttc ggtggtacga agttatttcc gtgagaacag tggaaattac
    18181 cgggcggtgt tgggatattt taaagaatac tggccgctgg ttgtgacaaa ctttctgtat
    18241 acgctcggac tgtatatcca caattttgtt ttctggacga cggatctgcg tatgatcgta
    18301 gttcgaagtt ttgtctgtgc acagccatat gatatggcaa cctgtctggc gatgttcacg
    18361 aatatttcgg cgagtgtcat ttttatcagc cgtgtggaaa tgcatttcca tgagagatac
    18421 aaagcctact cggaagccgt gatcggcgga agagggtgcg atattgaaat taccaaaaaa
    18481 agaatgttcc gtatgctggc tgaggagttg atgagccttg cccgtgtaca gtttattatt
    18541 acacttgtta tatttttgtt ttgtatgatc tttcttccaa ggcttggatt tggaggtatg
    18601 accatgcgta tttatccttg tctgacagcc ggatacttca ttctctttct gatgtatgcg
    18661 gaaattatat ttttatacta tttcaatgat ctgaaaggat ctgtgatgac cggtctgtcc
    18721 ttttgcctgt ccactatgat cggcagcatc atttccacgc atttttcgga aatctggtat
    18781 ggaattggtc tggtggttgg ctgctttgtc ggatggagcg ttgcctatgc gaggatccga
    18841 aatatggaaa aaaatctgga tattcatatt ttctgcaatg gacatttgtt gaagagaacc
    18901 aaaggtacct gcccgtcgcc gaaagtattt gatcgatatc agggagatac agaagagagg
    18961 atgaaagaat catgaaaaag gtactgtttg taatcaacac acttggcggt gcaggtgcag
    19021 agaaagctct gctggagctt ttgaaaagat ttcctgagaa cgaatatgaa gttttacttt
    19081 atgtactgct ggcacaggga gaactgatcc atcaggtccc gaaacaggtg aaaattttaa
    19141 atcaatctta ttcggatgcg tcggttttat cgaaaaaggg aaagcatatt ctgaaccggc
    19201 agatcttcaa acgtcttttt gtgagaggag ctattttcaa aaatcttgga tatatgctca
    19261 aaaatatggc agaaatgctg aaaaaaggaa agctttatcc ggataagctg ctctggaggg
    19321 tgatggcgga cagcgcacag gtgatcgatg gacattatga tatggcagtt gcctttctgg
    19381 agggcggatc aacatattat atccatgatc atatccgggc agaccgcaag gttacgtttc
    19441 tgcatgtaga ttacagttat gcaggctaca gccgcagtct ggatcgggat tgctatctga
    19501 attttgatcg ggtgtttacg gtatccgatg aagtaaaaaa gagttttctg gaagtttatc
    19561 cggagtgtag aagttacact ggtgtgttcc ataatcttct ggataacgaa gaaattatcc
    19621 gaaaatcaaa actggcagga ggatttgagg atgcttacga tggcaagcgg atcctgacgg
    19681 ttggacggct gacggcacaa aaggcctatg atattgctat tgatgccatg aagattttga
    19741 aagatcatgg tgtaaaagca aaatggtatg tactcggtga gggtgagtta agggataggc
    19801 ttcagataaa gatcaaccat cttggcttga aagatgactt tattcttctt ggggcaaaga
    19861 ccaatcctta tccttactat gcacagtgtg acttatatgt acatgcgacg ctgtttgagg
    19921 gaaaaagtat tgctgttcag gaggcacaga tcctgggatg caccttactg gtgtcagact
    19981 gcagcggaaa tcgtgagcag gtcattgatg gtgtggacgg aaaaatgtgc cagctgaccc
    20041 cggaaggcat cagtgcaggg atcgaagaac ttctttcaga cgaagagaaa tgtcgggagt
    20101 atggcaagaa agcctcagaa aagctgacag cagaaaaaga agatgtcctt cagatgtttc
    20161 gatttaacga taattaaggt gtgcagtttt tgaacgcaga gaagcggatt gtgaatgtcc
    20221 gctttacttt aaaataaggg acagatatga ggataaaagc aaatatgatt ggattttgga
    20281 agaaaaagaa agcctgggaa ttttcacagg aagttctgga agaaggagaa gaaatcctct
    20341 acgaaaattc ggagagagga tggtacgaga tctatacctt tccggtggaa gaaaaaatcg
    20401 atccacagga gttaaaatgg agtctgcatg agcaggaaac actggcactt gtcttgctgg
    20461 atcttcagaa attccgtgaa aaaccgctgg atgaacaggc gatttataac ataaaaacga
    20521 ttttatattt tttcgcatca cataaaaaag atgtgatcct gcgtcccgta tacgaccggg
    20581 aaggaaaagg gctggaggcg gagccagaan nnnnnnnnnc tggttctgac gcatctcata
    20641 cagatcgggg aagtgctgga aaaagagaag cacagtgttc ttgtttttca gggctttctg
    20701 gtgggaagct ggggagagat gcatacctcg gcatatctga cggaagaaca tatcagacag
    20761 atgtgggata tgctgaaaat acataccaca gacaagattc gtgtggcagt gcggacaccg
    20821 gcacagtggc gtactttgat cccggaagag aaatttcaga aacgggaatg gaaggcttta
    20881 ggtctgtttg atgacgggat tttcggatcg acaacgcatc ttgggacttt tggcacgatg
    20941 atgcgtgagg cggccggatg ggaaaagccg tggagccgaa aggaagagct ggaatttatc
    21001 gaacagatca gccgtgattt cccgtgtggc ggagaggcga tcgctgaggc agatccggac
    21061 agggcagatc agatccttac aaaggatgca aaagcggtga tatccgagat gcaaaagatg
    21121 catcttgctt atctgaatct ggtgcatgat acgagaatcc tgaatcagtg gaaagcacag
    21181 agctgcggca aagacggcat ctggagcggc aaaacgcttt atgaatacgt cagtgcccac
    21241 ctcgggtatc gttttgttct gaaaaaagtg gagatgctgg tgccaaaacg tgataaaata
    21301 aagtttattt ttgaagtgga aaactgcgga ttcgcttccc tgtttcagga ggcacagctt
    21361 tttttgatcc aggaaaacga aaaagaaaga aaagagatgc tgctgtcaga agaagtgcag
    21421 aagtggcatg gcggaagcac atacaaagtg gagacagaga ccggggcaat gaaaggaaat
    21481 atttttcttc agatccgcag gaaaaaagac ggaaaaatca tttcttttgc aaataaaaac
    21541 agtgcagacc gcctgctgat cggaagtctg cacagtgtat aaagccagaa agagttctgt
    21601 cggaacggta agagctaagt cctgtttcat catgggatca caagtcttcc gacagaaacg
    21661 gtggcactct cttcggaacc ggtgtttgca aaatgaatga cctgtccggt gtagggatct
    21721 ttcatttcca gatgcacctg ataagtgcct tttgcaaaag agcgtacatc gagttcaatc
    21781 ttaaactcag actgatcgct gccggcgatc gagcggttgt cgatctctgt tttcatccgt
    21841 ctggactcgc ctgtttcctg gttcgtgagg atcagagcag tatcaaactg ccggtatgcc
    21901 ggggcaaaac cgacgttttc aatcgtaaga tacagagagg ccttgtcgct cataaaggca
    21961 tggaaatcca ggctggatga ttttataaag tagcggtagc ccagatgggc acggatataa
    22021 tcgtaaccgc tgcatccgga aaatacgccg gagccggtgt aagtagaatt tttccattta
    22081 ttcagcacgg caagatcgtg atccgaattc aggtacgata cgcacattgt ggacagatcc
    22141 gaaatggcat tgtccaggtt attgtattta ttatcgatcg tgacttcacc gccgtttgga
    22201 acatactggc agagcttgtt ctggaaaaga agttcttcgc tccttgtgcc ctgttcgtta
    22261 aattcactgg cggaagtcag tggtgtatca tcatatgtac cgagatcata gacagagccg
    22321 agcataccgt cattgaaaag tcccagtctg gaagcaaggc tcccctcgtc gctctggcct
    22381 gccattggtg tacggctgta gagaatgccg cggagctgag ccggggtacg cacagcaaga
    22441 tagatcgaag gcgaaatatt ctttgccagt gtctctgcaa gctgacgcat ctggtcaatg
    22501 tctccgtagt tggtattgtt catctcgcca ttgttgccga taaaaagtcc ctgaaggata
    22561 taaatacagt cggcatgggc atttactgtg gcagaaacct gctggatgtg gtttttgatg
    22621 cggtaaaaat cagaaggctc cgttttttgg gctttgccgt cccagtcata gagaaagcga
    22681 agaatgatct gttttttctt cttttcgcag atcttaaaaa tgcggttaag ctgcgaaaga
    22741 gcgttggcac tgagatcctg atcagcataa ttccgcagat tgatctccag aagcatgaga
    22801 gggtacggag tgtccttaca gcttctctgg caccagatgt cggcatccct ggcagtttta
    22861 tcctcggcaa gtacatagcc gtttatctta tagaaaccgc agaaagggtt tgtaatatcc
    22921 gctgtagatt cttttgagtc tgccggtgta tagacaacgt tcagctgccg caccgacagg
    22981 gcataaaaag aagctgcgat taccagtgca acgcacagag taaagacggc gatattgccc
    23041 cagaaagata attttttttt cataaaatgg tttagctcct tttggatttt atgtctattt
    23101 gtcttttggc tcatgttatg acattggggt caaaagatgc catacgaatt gttccgtact
    23161 tcgtactcac acaattctgg catcatgtta ttatacctaa aaagaaagga ttcgtgaaga
    23221 gtgaaacaaa aatcaagaac agaatattcc gttatgaata caagtgtagc gtttgtggca
    23281 caggtttcgg caattctgat gggattcttt acaagagttg tttttacgag aatgctcagc
    23341 gaaggttacg tcggaattaa cggcctgttc accgatattt tgaatatttt gtcgttatcc
    23401 gagcttggcg ttggaacggc aatcacttat gcattgtatg ccccgatcgc aaaaggggat
    23461 attaaaaaac agcagattct gatgcggatg ttccgcagct tttatcgcat gacggcactt
    23521 tttgtcatgg gggcaggtct gtgcctgatc ccatttctgg atattctgat gaaagaccgc
    23581 ccggacgtgg atcatctggt gctgatctat tgtctgtatc tgttaaattc ggtggtatcg
    23641 tatctgttga tctataaaaa aaccttgatc gatgctcatc agatgaatta tattactgta
    23701 ctgtatcaca atgggttcct ggtgcttcag gatgtctgtc agatcattat tctgatcatg
    23761 acaaaaaatt ttgtcctgtt tctgagcgtt gccgtagtgt gtacgattat cggaaatgtg
    23821 tgcatgtcca gaaaagcaga tgcattgttt ccatatctga aggagacctg tcatgaaaaa
    23881 ctgccgcagg aagagcggcg ggatatttta aaaaatgtaa aagcgatgct gatgcataaa
    23941 cttggaaatg ttgtagtcaa taatacggac aatctgctga tctccagttt tgtcggagtg
    24001 gtcagtgccg gaatttactc caattatttc ctgatcatcg gttcgatccg tcaggttctt
    24061 gatcaggctc ttctgggggt ttctgcaagt gtcggaaatc ttggagtact ggaggataaa
    24121 gaccgggtta aaagcgtttt tgaacagctg ttttttatcg ggtactggct gtttggattt
    24181 tccggtatct gcctttttga aatgttgaat ccgtttgtgg aatttgcgtt tggcaaaaag
    24241 tatctgtttc caagagaaat cgtgctggtt ctgtgcatca atttctttat caacgggaca
    24301 agaagagcag ttttaatctt taaagagtcg atgggacttt tctggtatga ccgttataag
    24361 gcgatcgcag aggcattgct gaacctcagc atttccattc tgcttgtact tcagttcgga
    24421 attgtgggaa tttttatcgg aacgttctgc agcacggttc tgacctctgt gtgggtagaa
    24481 ccttatatca tttataaata tcgtctgaaa agaccagtat ctgagttttt taaaacatat
    24541 gcatactata tgcttgtcat ggcaggagtg tggacgattg cagaatgttg ctgccggatc
    24601 acaagcggat tgccgctctt ccttctggtt taccgtttct gtatctgtct gcttctggtc
    24661 aatctgcttc tgtgggcggt ttaccgnnnn nnnnnnttac cgcagaacgg ataactggaa
    24721 aagaacagaa gccctgctgt ataaagtatt tcagaatata tggaagaaag taaggaaaaa
    24781 gtgagtatga gtgaagaaaa aggaaaaata tctgtgatcg tgccggtcta tcaggcagaa
    24841 aaatatctgg caaaagcact ggacagtgtg ataagtcagg attacgaaaa ctgggaattg
    24901 tttctgcttg ttcgaaaatc ttcggatcaa agtgaaaaga tcgtcagaga ttacgagaaa
    24961 aaatacgaca gcatccatat gatagagcgg ggagaaaata aagccggtga ggcgagaaat
    25021 caggggctgg aaaaggcaca gggagaatat gtgctgtttc tggattcgga tgattatctt
    25081 ccggatgcat ccgtcatgca gcgttatgtc cgtatggcag agcagacaga tgcagatatt
    25141 gtagttgcca attatgcgag actctggaac ggaaaaatgc tgcctgcagt cagccacgca
    25201 tctttttcca gatatcatcg agattctcag gaatttcagt ttcgcggctt cttttccgcc
    25261 gggacacttt cttatgtctg gggaaaactt tatcgcagat cgtttctgga aaagaatcag
    25321 atccgtttcg caaaatatga ttatgcagaa gataaatttt tcaacatgca gtgttatctc
    25381 tgccgtgcaa aatatatttt cctttcccgg atcggctaca tttatcgaaa aaatgaaaca
    25441 tccatatcca gccggtaccg tgcaaattcc agcgagtgct ggattgcgat ggcacgcgat
    25501 ctgaaagaat ggatgtcgga tcatgaaatc cagatggagt cctgtgaaga acttgtctgg
    25561 tatctgatct tttttgcgac attttttgat gcaaaaatgg aatatgaaga acacaaaaag
    25621 aaactgagat ttatctggaa aacgctgcgg ctttatggaa aagatgaatt tgcacgggaa
    25681 tgtctgtgca gactggcagc agataaaaaa atctggaaat tacaggataa gaaatgggtt
    25741 gttatgacca aaggattctg tatcggtatg aagcttaagc tttatctgtt gctgtccatc
    25801 ggaatccgca gcctgatccg gcgaagaata gatgagaaat tatctgatac cggactgcgg
    25861 gaagtgtaaa tacaggcagc gactccgtca ggaatcgctg ttttcaaaaa tacggatcag
    25921 ctgtgtcttc tgattgcttt ctttgaatcg ctgcaatgtt tcccggaagg tggtgtttcg
    25981 gagcgtataa gtatttttca gaaaacagaa aaacgtaatc ggccacagca tcggccacag
    26041 aagcggacag cgtcctgctt ttggaaaacg tactttcatc tgcaaatgtc cttcccggaa
    26101 ataagtaaac agatttactt ttttataaga atgactgcca acaagttcct gtgatacatc
    26161 cgcaccgaga tccccatcct ccaggatcct ggacaggaaa gcatcgcagt cggagagatc
    26221 gtagtgcaca tccggatcga tcatctccgg taaattcaga tatattcggc aggtttctag
    26281 gacgctctcg tagagatgga gaatgtgcga ctgctgacac cagctgtgga tctttgcaaa
    26341 atcaacctcc tgtgcatgct gctccagata aaaagaaaaa tcaaacagca gccggatacc
    26401 aaatccgctg tagagataat gtttcagcat atggtggatc atgtaaaaca cgtattccgt
    26461 aggcggaaat acatggtaag aagatccgtt gatttcctgt gtttctgctt tcagattttc
    26521 agcggaaaag acttcatcga tcagctggtt cgcaggttta tactgatata ctccaaccac
    26581 acggtaatgc agctccagaa gaaagctgcg tcctgtcttt tcaaacgtat agcgataagt
    26641 ggtgtgatga tcagaaagtt cttcttcatc cagatagccg ttttcatgca aaatctgttc
    26701 tgcacagtga aacgcatctt tatcattaat atagagatcc agatcgccga gtttgcgata
    26761 ttcaggaacc ggataagagg cagcaaggct gatccccttc ataacaaagc aggtaatgcc
    26821 atgcttttcc agaagcgata ccgtgcgacg tgtgaactga tcgatctgat agtaattcag
    26881 catcatgtgc ttactcttct gaagtatctg cggataaaga ccggtatttc ggaaataagg
    26941 aagtacaaac ggaagcgtac agtgacggtc tgcaagtgca aaaagtgcag gataatcttc
    27001 cggtgaaata tccggcagat ccgaagaggg atcatcaaat ggcatggtga gcatttttaa
    27061 gtaagtgttg tataatgaat ccatagcagg tggctccttt gatcatggta tggggactga
    27121 ttttcacttt tatctcagtc gagaagactc ccacttctgc aagcggtgag taataacaag
    27181 tctttttcag tggaagtaca atctccgact gagataaacg ctccgcgaga atgtgcagtg
    27241 attctgagaa gaatatgtca gctttcgctg accagaatca cgcaccaagt ctcacgtgcg
    27301 ttcgacttga aaaagtggcg aacaacaaat ttgtcttagt tatcataaca tttataaaat
    27361 aagaagaaaa gaggaaaaag gaatgaaaaa agcagaaggt tacgtgatac gaaagttaga
    27421 agaagaatat gtgctaatcc catgcggcga gcgtgccgaa gagatgaacg agacaatctc
    27481 cttaagtgaa acagcgggat ttatctatat caatgctgac aaagcagaca gtacagagga
    27541 gctggcaggg ctggttgcag aagaatacga tgtgccaaag tcagaagtat tggaagatgt
    27601 aaaggcagtg gtaaaaacat tacagcagaa aggaattctg ctgtaaaaag aatctgctat
    27661 ttagatcccc cgcaaaaaag cagtgatgcg aaaacagaca ggacgcagcg gcagcataaa
    27721 aagccacaga gcagagagaa agcggtacag cggattcttt accgaaattt cctttccatt
    27781 gcgtacaaag gcgatcaggc ggcctttgat ctggtcggca cgcagcggac cttcgatttc
    27841 agattgattg tctccgacca taaagaatcc ctccgcagtg atcttacaga tgcggtgcag
    27901 gacaaggata ctctggtcac ggcggtaaag aacgacatcg tttttgcgga ccgtgtcggc
    27961 agagacggat tcgatcaggg cttcatctcg ccccgggata aagagcgggt acatactgta
    28021 gccctgtggt ctgatgcgga tgatgttgcc gtcctgaagc aaagcttcaa gatcaggtct
    28081 ggaagatgcc atgaagaaac ctcctgtgga tcgttgtttt tggtcttact gagttttgat
    28141 tttagcattt tcagagcaaa aaaacaatgc tgtaacgctg taaaagatat gggtgtcaaa
    28201 actccccaac tatgatgcct acggattgtt ccgtgcgttg cactcccaca atcctttcta
    28261 cgctccgctt cggtcggtgc taaacatgtg ccactggcac atagcacccc agtattcgca
    28321 tatcgtggga ttatcatccc acttttatgc tcatatcggg gcgggtccca aggtctttca
    28381 tccacctatg agcaagctca tgtggattta gaccttttga ccctggcatc ataaaaaagc
    28441 ataaaaaaaa tattgacttt taattcttac aagattaata tatgtttaaa aacaaaggag
    28501 gtaaaagact atgagaacat ataaaaaacc agtcgtatct gtggatgcag gaatgacaga
    28561 aggtgtatat gcagcaagtg gtgctgcaca gggaaccctg aatgttacat actacggtgt
    28621 atgggacaga tggggaacaa atggcggtaa aggacttgct atggcaaact ggtccggtat
    28681 cgacggaacc attactctga acgttcattt taacgataca gtagatcagg cagagacaga
    28741 cgatgcatct gtacaggcat catgttcagg aaaaactgct acttttgtat ttgcaagcac
    28801 agcaacaaat ccgctgacga tgggtatcca tctgaaccat ggaacaagca ttgataatct
    28861 taaaatgaca agctttgaat attctgtaaa ataatttcag ggtaaaagat attgggtgat
    28921 ctgttttaag tgtcggtatc acagacggat cacgtacaga agcatagata aaaagacaaa
    28981 accgtttctg gttttgcctt ttttttcgaa aaaaatactg tgatcagaaa tctcccatca
    29041 gtttttcaat ctcttcatct ttcagcactt cacatttttt ttcacgtatc gcataaacac
    29101 gagtgcagat attgagtacg gtcggtcggt gagtcgtgac gatacaggtt ctcggatagg
    29161 tatcctgcat gatgtttttc agcaccttcc gttcggtagc gacatcgagt gcggaggttg
    29221 cttcatcgag cagcaggatc ggcgagtgtc gaagcagtgc cctggcaatg gaaagtctct
    29281 gtgcctgtcc ttcggagaat ccgccgccgc gttctttgac catgctctga atgccgtccg
    29341 gaagcttttc cacgaaatcc caggcacatg ccagctgcag ggcgtggatg atttcttcgt
    29401 cggtggcatc tggtttgacg tttcgcatat tttctgcaat cgtaccggaa aacatggtat
    29461 ttccctgagg tacataagaa aacagttttc tggtggaagg tgacatttca atctgtgtcc
    29521 gatggttgtc tccggcacag acatgatacg aaccgcccga tggctggagc agcgacagga
    29581 tcagccgcag catcgttgtt ttgccctccc cggaagggcc gaccagggca acgatctcgt
    29641 gtggataagc ttctatggaa gcatcgcgga acacttcggt tccggtgttg taactataac
    29701 tcagatcctg catggcaatg ccgatgcctt ctgatctgtg atctttttca aaggcaatga
    29761 cctgggcatc atggctgtaa tcctcctgcg gcatttccac aatatccatc agacggccgg
    29821 cagagatcgt cagggaaact gcagacggaa caaggctggc aagcgaattg acagagcttg
    29881 tcagagtccc ggacagggag aggaacatgg tcattgttcc gtatgtaatg gcattgctcc
    29941 agacgcggta aattccccag ccgtagcagc tgtaggatac gataaaaccg atcagcgtca
    30001 tcaggatgga agtcagcatg gacattttct gaaaatccat tcgcatctgg atatattctt
    30061 tttgcagaga tttcaggcgt tcgatgtaaa aatggatcag gtcaaatgct ttgatcgtct
    30121 ggatattgga aaacgtttcc tgattaaatc catacatttt ggcgttcata gcggcactct
    30181 ttgcattgtt gttgctcatg cggcggagca gtggttttga caagatcgca ctgaacggaa
    30241 tgccgatcag ggcaaacagg gcaaaggtcg gatcgtagta gagaacgacg gcaagtgcac
    30301 tgataaaacg gaaaatgtaa atgaccaggt tcgggatcca gttgaggatg ccgctggaaa
    30361 tattcgaagc atcggagctc cagcgggtga ccagatcacc ggtgtgatag tcggtcaggg
    30421 actcccagtc ggtaaccagc atttttgcga aaatgtcatt tttgatctct gaatcgactt
    30481 tcaggttgat gaatgtcgaa gcgtaagtgg atgcctgtga aaataaaata tttgcaatgg
    30541 aaaaaccgat cattgcggca aaagtggaga gcagtttgcc ggtctggtgt ccggtgatga
    30601 tatctattaa atcttttgat ataaaactgg aaaccagtga tacgccggaa ccggcaatgc
    30661 cgagcagtgt ataaacaacc atggatttcc agtagcgttt tccatactgc cagatccatt
    30721 tggactgcag ccacattttc tgaagattct ccggtttgag gtgcttccat atcagcagca
    30781 gtttatcttt cattgtttta ctgcctccca cagatcgatt ctctgacgca ttttgtgtgc
    30841 cgcagaagga tttttcatac aaaggaaatg gaagaccgga atctcatctg tcagtgtttc
    30901 tgcaaatgca aggctgcgtg agaccagctc atctgtccag gacggggaga tcatccgctg
    30961 catgacacgc agaactcgtt ctgctggagt catgatctca aagcggttgt cttttgcatc
    31021 gcgtccaagt agtacaatac cgccaagcct ctgtttttcg gtggtacaga tgccggaagt
    31081 tccgcaccat ggaataccgt aaacaaaaaa ctgcccgttt tcttcgccga tcaggtttaa
    31141 atccccgttc aggagcggtg tgccgaaaag ctccttccag agattggtgt gagtggattt
    31201 tcccatgccg gagtgaccgg aaaagagcca tgccttttct ttataaagaa gcgaagcaga
    31261 atgaacggcg aattttccgt ttttctgagc gataaaaagg aagaatggcc gaattgcatg
    31321 gaaaaggctt tcaaggttgc tctctgttac ggcggaagaa cagtaaatct gtacaagatg
    31381 gccgtctttt gtcatatggg cttcaaaaat atttttcagt gtcggaaaca gcatcacata
    31441 gccgtcagca tgttcgagga tggaaagttc ccggtttcga agcagtacgg tgccgttttg
    31501 ctttgtcgga ctctgacaga tcagatggat ttcctgtgtg attttttggg ctgttttggg
    31561 atcacaggca aaagcagaaa attcttttgg aaaaagttct gccggtccgc acagacggat
    31621 caccagatct gcaatgttca tatatataga agaaagagaa tcggcagaat caccgtcatc
    31681 ctttaaaatg tcaaaagaag taagctgatt cagaaataaa gttacgtcct cttcaagcat
    31741 cggaaagtcg gattcttcca gctgataatg ctcggcaagc ttttttacaa gttctgcatg
    31801 ctttgtggta tcacagagac attcccacag aaaagcgccc gtttcattga gaaacagtcc
    31861 ctttttctga tcggcaacct gctgaccgaa cggaagaaga tagtaagaat ctttgatttt
    31921 tttcaggacg aaccccggtt gttttttaag cataattttt gtctcctttc aaagactgta
    31981 cggggcaatt atataacaat cgggacagaa ttgacagaag aaaattttcg gtctttaaga
    32041 atgattcgga taggccggat gaaagcataa cagcaggatt cagatgcaaa aaaaactcat
    32101 ctggccggtg tgatgcagat ttgcagcaga tggtattctg tgttaaaata gaggggcgaa
    32161 aaacgggaat acagggaaag atggaaaatc attacagagg tgaaagagat ggaggaaaat
    32221 gcaaaatcag aggaggagtc attcaagact ccgggggaac gtatcgtaaa actgtgtgaa
    32281 aagcagcaga tctcccagag ggagctggca aggaggatcg gtgtgacatc ttcacagatc
    32341 agccgtattt taaaagggga gacgaaaacg atcaacagtg atatgttgat ggctctggca
    32401 caggagtttg gcgtatccac ggattatatt cttggaattg tgaacaatcc ggaaaatacg
    32461 caaagtgtgg aaaatacaga aaacctggca gcagaatcaa aggacatgga ggaacgcagg
    32521 gaacagccgt ttcacacgcc gatgcttctg atgagttctg cttttccgct tggcggctgc
    32581 atagatttta ttgagtcact tgcagatcag gacgaaaaga tgatggcgta cgcagaatat
    32641 tattatttca gcggccatca cgagaaggcg gtagaatatg cggaaatgta tctggatcat
    32701 ccggatatca tgctgaagat gtcggcctgc ctgatctata catttgcaaa tctgtcgctg
    32761 aaccatatcc attcggcacg gatggggctg aacaggctga cggagaatct caatcaggtg
    32821 atgaaaggaa atacggatcg gaaagcaaag gcatttggtg tctttgtagc tgtcgcggca
    32881 cacacacttc tgcatcttcc ggttggcgat attccgccac tgtcagatta tgtcggagaa
    32941 ttcaaaaaag gaaaacagct gtggggtgtt tacgtgcttg cacataaggc atatctggat
    33001 cgggaatacc agcgttctct tggcatcgtg gaggcatgtc tgatgacgac aaaagagata
    33061 tatccgattt caatgatcta cctgaatctg gtggcggcaa tggatgccat gaatctgatg
    33121 cagacagagc ttgcaaagaa atattttatg caggcgtggg agatcgcaag accggatggt
    33181 ctgatcgaag gcatcggcga acaccacgga cttctgcagg ggctgatcga gacttgtctt
    33241 aagaaagatt atccggaaga ttatgagcgg atcatcgcaa ttacttataa gttcagtttt
    33301 ggctggcggc ggatccacaa cccggacacg aatgaatatg tggcggataa tttaacaacc
    33361 acagagttta cgatcgccat gcttgcgagc cgcggctgga caaatcagga aatcgctgat
    33421 tatatgaaga tcacgttaag aaccgtgaaa cagcatatga cctgtatttt caacaaaata
    33481 agtatctcta accgaaaaca attaaaagaa tacatgctga gatgatgccg aatgtttaaa
    33541 gaatagcggg acttactctg taacggagca attcgtagca tcattataaa ataaccagac
    33601 actacctgaa gttaaaaaag aaaatttttc tttgtaaatg gacattctga agaggaaatg
    33661 tccatttttt ttgtcaaatg gacgatgtct gtaaaaattt tttgaagtaa aatataaaaa
    33721 aggaaaatgt gccggggacg gcacagggag aaaattaaaa cagaaggagg cgtagtttat
    33781 ggagaaatta aagccaaaaa tctcgaaaaa agtcaaaaaa gttttaagtt ccgtgctggc
    33841 ggcaacgctt gtgcttggaa gtgttccggc gagtaccttt ttgggtgatg ccatacaggt
    33901 gcgtgcggcg gcgtcttaca gtccgcagaa gtatggtgtg ggcgtactgt gccgggatct
    33961 gacgaccgga gacaaggcaa aggaaatttt caatgcgctg gtacggtgca agtccaagaa
    34021 agaggattcg ctttacggca gtatttacaa caatgttggt atggtgaaat acaaatacag
    34081 caagacgaaa gccggctccg gggagacatt ttcagtcaaa gtgcagaagg actatccggt
    34141 gctgtacaat cagtccggac agatcagtca gaatctgatg gcatacataa cgaatgataa
    34201 acataagaat ttcggacatc actggggaaa ccggacgcag tacgggaaaa ttatctttgg
    34261 ttatccgacc aatgcaaata ataacagttt ctggctcggg cagagctaca atacaggcga
    34321 tcataattca gatggaagct ggacggtcgg ggatcgtgat acctgggcag catttcggcc
    34381 atcgctggga gaaaataaaa gagcagctct ctatgtgact acgaagaagg gctgcggcag
    34441 ctgcggaagt gccaagatcc agaatgtatc gattgcattt aaggatgaga ccgctccgaa
    34501 gatcaagagg atcacgatca cgagtgataa aaactctgac acaccggcct tgtattttaa
    34561 ggaaggtcag acgatttatg taaaggtgaa attcagcgag tatgtgcgtc tggcagataa
    34621 cagtgcaggc agtacacagt caaaaaacat cagactggcg ttgtcgcttg gacagaaaaa
    34681 tacggcagat gtggcaactg cgtatgcgaa tctggatact ttgaaagatg atattgcaat
    34741 attttcttat aaagttccaa aaaccattga taacaaaacg gtagattttt atgtgtccgg
    34801 acttgcggat atcggctatc agacatcgat cattgatcag aacggcagca aaaattatga
    34861 ccgaaatttt gtcggttatg atgggggtac cttgtctaat ggaagtggga cgatgaaaaa
    34921 actgaaagca gctgttggga gttccaatta taacacactg aaaaaaacaa gcagtgccat
    34981 cacagacctt gccggcaatc cgctcagtct ggacggattt cgcaatgtga aaaaaggatc
    35041 cgcaggatgc agcgtgacaa gggcatacct ggatacggta aatccgatcg tcgaaacagt
    35101 ttcgatcacc acgaaccaga aaaaccagac acaaaatgca aacgaagcca attatctgaa
    35161 ggccggaacg accgtgacgg tatctgctgt tttaaatgaa cgcatccaga agatcagtga
    35221 tgccaaccgg aaaaagatca tcgcttcttt aaatatcaaa aagaaagacg gcagtccggt
    35281 gaaacttgag gcaaaatatg tttttgatca gtcgtcaacc ggaaaaacgg ttgtcatatt
    35341 caaggatttt atcgtggatc gggatatggt tttccagaag caggataaat tgaactacaa
    35401 aaagaaaacg gattataaag tgacgatcac ggaaattgca ggcctgagcg agatcaaaga
    35461 ttattccgga aatacacttt ctgtggcaaa tgatctgaga ggcagtacaa gtaaagattt
    35521 ctatctggat aacgaagctc cgacgatcat gatcgacagt caggctgtat cagaaacaaa
    35581 aacgtatgag atggcgagaa tgacgaagtc agaagatgaa gtgtattctc tggcgttcag
    35641 cgtaaaagat tatgatacgg tgagcggaaa tacttccggg cctctggcat cgggtgtgat
    35701 cggcggaaaa gccagcatga cggtgaacct tcagggacag ggagatgcca ataaattctg
    35761 gtattatgca tcacagacgg cactgaaaga agatgagatc gaccagacag gattcaaaga
    35821 ggcacagtct ggaaagacaa agatcgatct tgatctgacc aatgatacaa cagatcttca
    35881 tctgtatctg aaattcgaca aaaatattga ttattcttcc gtaaacaacg gagtcaatgt
    35941 gcaggtatct gcaagtgata ttaatcataa tacaaagacc gtaacagcaa aatacagcta
    36001 cacggcaaaa gacaagatcg ctcctgtgat ttcttatgag agcatacagc tcaaagaaaa
    36061 caatgacaac agtgcatatc aggaagccgt tgtgagagtt aaagatgcag gcggcatcaa
    36121 tatcgacaac atccgctact tctggctgga agaaggcagc acgcttccgg gaagtgacaa
    36181 atttactacg gcagctgtgg gggatgtgac ggttgtgagc cagtccggcg ataaagtgac
    36241 cgagtgcctg gttacgattc ggacgaaaac agtcggagca aaccagactt atcaggcagc
    36301 actttatgtc tctgcagccg atatcgcaaa gaatgaaacc ataagcgaaa aacttgcaga
    36361 tgcggcaatc gaccgaaata ttccggcagt ggaaattata acaccggaaa caaaaacgaa
    36421 tgaagcaaac aaagaatctt cgctgaaagt ccgcggtccg atcacttctt ccgcaaatga
    36481 agcggaactg tttgtggcag taaaagatcc gatcgaacag gatgcttatt ttatccgcag
    36541 cgtgtcagga aaggcatcag aaatactgat cgataacgaa gaatttttgg atgcggcaaa
    36601 acttccgtat tcttccggat atcagagcag cgagaccgga aaatggtcct acggaacgct
    36661 tatcatgacc ggagatcaga aatatgagat cacgacaact tacaacagtt taaatgatgc
    36721 aaatgcggcg accgtaagag aacgtttcct ggcgatcagc ggaaataaat actatggaaa
    36781 tgtaaccgtt gcagcagggt ccggttttaa gagcagtgct tttgcgacaa gcggaaataa
    36841 aaaaacattt gatgcatcaa aaggaaaact ggtcacaaag acctttacca tgactcctga
    36901 cgagtatgga ttttccgata agaaagatgt aaaaaccgga agttatcaga tcgaccggac
    36961 accggcaaat accgtgacga tcaaaacaaa ggataatgca aattacgggc agcaggtaaa
    37021 acgggaagat tttggagatg catacaatcc ggaggtaaac ggagcagaat atctctccac
    37081 acttgccggg gcagcatttg acatcagctt tgcaaatcag agaaccagcc attttgtgat
    37141 ggatgatatg gattatgcat cgccgaatac ctatatctgc ctgaaacaga cagaaggtga
    37201 taaggaaacc gaagtttacc ggtggaatat cataaaggga aaatccagcc agacggtaga
    37261 aattccggaa ggtctgacac tgccaaacgg acagtataaa ctggaagttt ccatcagcaa
    37321 caaacagggt aattcggcag aaccggtcat tacgacggag cagtatgaca acatttatct
    37381 ttataattat gatgctgata tgacagaagc atttggtatc agcaaagtga cgggaagtat
    37441 tcattttgca gcagaagatc catatcaggg agcagataaa gttccgtact atggatttgc
    37501 cgcaaccgga acgtatgaat ttaaccggac cgttgtggat gaccagcaac cggattttac
    37561 cgcaaccgga gcggatggct atacgacaga tgttctctat cttggaaact gtgcaacatc
    37621 tgagacaacg gaagggatta cttacaaccg cagcattcag tttacggtaa acggcatgga
    37681 agattacgat gtcagcaatt actggatcaa agtctgggcg ggtgataacg aggcttctgc
    37741 gaaatggttc ccgcttaagg attatgatgc agaaaataaa gtgatgatgt ttgacgtaaa
    37801 gccgacggat gcaaaagcaa tgttccaggc ggaggatgcg gcagcatttt atgcaggcgg
    37861 atatgcaaat gcagatgtat gtgcagttcc ggtctttgaa ggcagcaaca cgatctctta
    37921 tgaactgatg aacgttggag gcgtgaagtc acagcgtcac gaagtggaga tttattattc
    37981 tgcaaaagca ccggatttca aaatagaagt ggaagaaagt gaggcttcca ggcagagtat
    38041 ttctgcagca gtaacagaag cagacagtac gatggttgca aaagatccgg aaatgtatga
    38101 agtgaatctt tctgcttccg atgacactgc ggatacgctg ataaaagaga gaagttttgc
    38161 ttatacagaa aaccagcgac acctgtatta tctgctggat ggatatggaa acttaagcta
    38221 tcagcagttt tatatcacag atgtggatca tacagcacca acagcagagt ttacagtgtt
    38281 aaaaagtgcc acaagcgaga ttactgcaat cagcacagac agtaccgcac agaacgaagg
    38341 agaaacggat gaacatatca caaatccgag ctttgaaatt gagatccgcg acgatcggaa
    38401 tctgaaaaat gcaaatattc agctgatcat cgatggaaaa gatccgatcc atgtggtaca
    38461 gacagacgat ggatatggat ccgactggga tggctcttat tactgcggta tggagttcac
    38521 aaaggatatc aaccagagtc taggttttct gagtgaaaat tatctggtga ataaagatgg
    38581 cagccacacc ctgcgtctta tgggagattt caaggcagac attgatcttt ccaaagagaa
    38641 aggacttcat atgccgcaca gcattgagct gaggattgtc gatgaggccg gcaatgtcat
    38701 ggaaacgatt tcttccaaag accaggactt ctacggaatc aaccagcggc cgaagatccg
    38761 cagaatggat gtttatccgc aggacggcat ggtaaacttt gaattcaata tggaagtcca
    38821 tatcaaaaaa ataaatggga tcgaacttcc gcagatcatg tacagataca tctattatgg
    38881 ctcgtacttt atgcttggaa gggcaagaga tatgctgcgt cagtttgatg tgccggtgga
    38941 cgacgaaaac gtcttgctga aggattactg cggaatcagt aaggatggaa cttacgagat
    39001 cgagtatgaa gatatttatg gaaattcata tacggagcag tttacactgg aggacttttt
    39061 cggagattat tcggcacaga tccagtacag cacgacagag aaaacaaaag aagatgtagt
    39121 ggcaacgatc gaaggtgtca gcgaaaacgc acagatttcc ctgaaaaaga cagacgatac
    39181 aggtgaaagt agtacggatg acagcgaaaa caaagaagat accagtgacg cttatacgat
    39241 cagctggaat aagcagaaat caaaggcaac gatcgtgttc cacaaaaacg caaatattac
    39301 atttgaactg accattccgg gagcagctga gcagaaaacg gtggactaca atgttacggt
    39361 agaaaacatg gataaagatg caccgaagga tgcaaaggtt tattggagat ttctggagaa
    39421 cggagaagta caggaaggaa atacactgga tatttcaaat cttgaagatc agagtaccac
    39481 agacggtatt gaagtctgga tcgcttccga gagtgaagat ctgtatgctg caaatggaaa
    39541 ggaattaaag cataccttcc tctattcgga aaacatggaa cgaagctata cctttgaata
    39601 cagtgatgag tgcggaaacg aaggtgcacc gatcacggtt acactgccgg atgagctgaa
    39661 catgaagccg tatgaagcac cggtggaaga ggaaggtgct tatgaaaaag acacaacacc
    39721 tccggaagtc gttgcggaag tctatgcggt ctatgacgga ttggcaagtt atttcagttc
    39781 ctggacaccg tcagaagatg cttttgccga actggcagaa aagatcggat acagcggcgg
    39841 ttataagatt tcttatacca tttctgatga cagccggacg aaactgatcg tgaaaaacgg
    39901 actgcaggca gatacgggaa aactgaattt caacagtacc tcagatcaga ttgatggtgt
    39961 gaaactggat gcgaacaata acagcctgct tatcacaaag ccgtgtcaga tcacggtgat
    40021 tgcgatcgat caggaaggaa acttattctg gcacagtctt gaggctgcga agatcgacca
    40081 ggaagcaccg acggttcgtg tggaaaaaga aggtatcagc tttacccgca tgagactgaa
    40141 attctatgca gatgataatt ctgataaaga caacgaaaaa ggaaccatcc ttccggtgac
    40201 gtcgggactg cagaagggca tggatgacaa aggctattac tatttccggg aagtagaaaa
    40261 taacggaacc tacgatacta tttttaaaga ccgaagcggc aaccgggcaa agatcagcac
    40321 aaaagtaacg gagatcgaca aggatgcacc gaagatcagc gtatcttcct ggagtccgtg
    40381 ttacgtcaaa gacggagaat cttatgaaaa actgccgccg atcgaaccga caaacaactc
    40441 cgttctgctg agtcttgatt ttaacaagac agtatcggaa ctgaaagtat actataaaca
    40501 gaatgataac tgggtagaag acaatggaac attctcaaag acaggaatcg agctgggcgg
    40561 aagaaaagga aacgtagaat ttttcgcagc ggttccagga atggttaaga tcgtggcgac
    40621 ttcgccgaac ggtgtttccg gtgaaatgac agatatcgac ctggttgaca tcattgataa
    40681 aaatgcaccg acgatcacag tgacacagaa actggaaaac aatcagatga acgttatctt
    40741 ccgctccgat gaaaccgtat ttgtaagcgg cggcggtctg aacagaaaat atggcggcaa
    40801 caccaatatt tctctggcga tcaaagaaaa cggtgtatat gatttcacat ttgcggatgc
    40861 agccggaaat atgacacagc agcagatcac ggtggattcg atcgatgaaa tacctccggt
    40921 tgtctacgca tccggcattc cggaaacgta catccgctca gaggactgca gcgtaaaggt
    40981 tacgatgtcc gaggcaggaa cgatcacgtt ccgcggaaaa gaatatccgg tcaaagcacc
    41041 gacggacaaa aacggcgatg gcgaattaaa aggagatgaa ctggactgga tcacacttcc
    41101 gatcacgaca aacggaagtt atcaggtcaa agcaaccgat caggcaggac tggtttctta
    41161 ccgtgtactg gagatccagt atctggatga tattgcaccg agtattcagt ttgaccgctc
    41221 tgttgtaaat gttttccagg gaacgactgc cgaagaatta agagaacagc ttctggatgc
    41281 gacgacattc cggctttggg ataacattga tccgaatccg cagatttcac tgaaaaacat
    41341 gctgacggaa aaacagatga acgagcaggg tattcatgaa gtcacatatt tactgagtga
    41401 ccgtgtggga aatgaaaaac tggtcaaacg gtatgtgaaa attatcagct cggcaaatct
    41461 gaagatcacg gcaaacggcg aatcactgct tccgtgtgat acaacgatcc tgaaagaaaa
    41521 tcgggcagat ctggtactgg aaaaatccag aagaagcggt gaatccttta aggtatatta
    41581 caaaaaggga attcagaaag ccggagcgat gaagaaggca gatgttgtga aagacggtaa
    41641 gctgacggat ctggaggcag gattctatac gctctatatt gtaacgcaga ataaagaaac
    41701 gtatctgact tatatttata ttgatttaga acagtaaagg agggcaggta tatggataag
    41761 agaaaaaaca agatattttt aaagagggct ttcagtctga tgctttccgc ggttgtgctc
    41821 tttacgggcc ttccgctgga aatgctggcg gcaccgcaga gtatggatag taaagtggcg
    41881 gatgaagatg tgctgctttt gcagaatgat tcgatatctg tccgcatgtc aggtaaaaac
    41941 ggcggatttt atatctcaaa tgtggaaggt gataagacaa taaaatccga caataacaaa
    42001 gatctgctgt accacagcga tgattatgac acttcattta cttccttccg cataaccaga
    42061 aacggagaga caaaagatta cattttcggc ggagattatt cttttgaagg aataaagtcc
    42121 ggcggtgtaa ccgtttcgca ggatgcaaag ggactgtcgg caaaatggag cctgggtgaa
    42181 ctggaattca cccagcggct cgagctggca aataccggat caaacgaaca cggcatggtc
    42241 atgatcaact acgatgtaca gaatcatggc agtgaggacg taaaagttga ggcgagaatg
    42301 ctgcttgatt cggccgtcgg agatcaggat tttgtatatt atgaaattcc aaatacatct
    42361 tatgacagcg atattatcaa acgggaatgt gtgctggatg cagcgaatat tccgactgcg
    42421 ttttatgcat acgatgacat ttacagtccg acggcaacgg catcnnnnnn nnnntcttcc
    42481 aaaggaatgt taaaaaaagt cgcttttgca cactggaact ctctggcggc gaccggcttt
    42541 gattttgccc cggatgagac tctggatttt acaagcgacg gcaatgatca gtacgggaca
    42601 gcagatagtg ccatggcaat gtactatgat cttggaacaa tccagtcggg aaaagaagga
    42661 attgtaaaca catactatgg agtattttcc aatgaaaaaa cagatcttga aacagatcag
    42721 gtaaccttca atatgacttc accgtccgtt ctggatctgt cggcagacgg aaaaaattat
    42781 gtttctacct gcaaccgcaa tacagacgga acctttaaag aggacggcat cattgatatt
    42841 caggcaactt taaaaaatat ttctgagaag aacgattata aaaaagtagt catcgcagct
    42901 tatgcatcgg aaggtctcac accgcttgac gaagatcaga aagagccaat ctacgagacc
    42961 agctatgcag tgccgtatca gagcacttat gtggatttca cgaggggaac ggagatcaag
    43021 atcccgtttt atttccgggc gaaagtggac agtgcatcca gctatcgaaa gattacgttc
    43081 cgtgtgttta accttccgga ttctacggat gcgagactgt tatatgaaaa tctcttgtat
    43141 gaaggttcta cttacattct ttgcccgggc ggaaacggcg aactgccgaa gatcacgttt
    43201 cacagtgcga caccggagat cctctataat gatctgacga gacatctcta tgtgagcggc
    43261 agcaatttca ccatgctgga ggataagagc cgctacacac tgtatgcaca gggagcaagc
    43321 gacagcaaaa agtacgaaat tccatcagaa aacattttga tcgatccgca ggaaaacaaa
    43381 ctggatattc tttttacgga gacgatgaaa ccggacacat atcagctcag ttttgagtgg
    43441 acagaaccgc cgaccggcgt tgacaagatc acaaccggcg atgctctgaa ggtggtcatg
    43501 tcggatgaca tccgctatcg aaatgattat tacgggattc tcgcagttgt gcaggaaaaa
    43561 ggcaaannnn nnnnnnaagg caaaacaggg gatcaggcaa aatatcagct gaaaacctat
    43621 gcaagcgaag aagcttacgc agcagataaa aacaattacg aagaaacgtt gctgttcttc
    43681 cagggaagct ttatcaaaga tgataccttt gataaaggtg cggacggaac aaatgtgaaa
    43741 tacgttgcaa aatcgatcag cggcgaaaaa gacaaagttg tgatcaacgg ctgtatcgat
    43801 gccctgaacg gtaccgtaga agtcagccag aaggacggcg taatcaacac agattttaat
    43861 gatattacat taaacgcttc tgtagaaaac accaggatct acaagggtaa tgctgcgttt
    43921 acaagcatcg aaaacggaac agaatacgga cttgtgcctt ataacatgaa cggagaagag
    43981 atcgaaggtt ttcaggatga ggagatcacc ctgatttatc cggcggcatt aaatggtctg
    44041 atgacgatcg ctgggatggc atttaatctt tcttttgcaa ggcttggcat gatgtatgac
    44101 ggcgatgcag atcgggtgga agaactgaag gtggaggatg cagaaggatt tgtgatgtca
    44161 ttctccgcac agctcgactt aagctttctg atcccgacat ccaaaagagg aaacaccggg
    44221 gaaaaggttg ataaagtaaa tcagaatgta tttgcaaatg acggagcaaa agcagatacg
    44281 ctccgcaatg cgtataaaaa aacttacaaa ccgaccggca gctcttccgg ttctcttaca
    44341 aaaaacaata agaaaacaaa agtcgcaaac aaccagaata acaaaaaaga aggggatgat
    44401 gatggcgata ccgatgcaaa agtagaggta aaaaatgtac tctacggcat gaatcaggga
    44461 tttatcggag tgaatttctc agccgaagta aaaatcccgg gctacaccga ctccatgccg
    44521 aacatcgaag gaacgctgga ggtcaacac
//
LOCUS       DAWDWH010000002        35032 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_686_length_35032_cov_15.211225, whole genome shotgun sequence.
ACCESSION   DAWDWH010000002 DAWDWH010000000
VERSION     DAWDWH010000002.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 35032)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 35032)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..35032
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_686_length_35032_cov_15.211225"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    21881..21890
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         9137 a         8329 c         6551 g        11005 t
ORIGIN      
        1 aaagagccgc tgtctgagcc tcgaagaggc gagtttaggc gaagggcggc taacacacga
       61 gcgaaccgaa caggaaacgc agcgtgtcag gagattgttt tcgaatcatt atggcgtgct
      121 attttacaca actatttttt ataacattca tttgtacatt ctaaaacctc cgctgtatac
      181 tttcgccgat atcacagaca cgatcggcac agtcagtaca atccccaatg ttcctgaaat
      241 tccttttatt acttcaatac cgatcgaata catatttact acctgaaggt acggcataat
      301 gtatgcataa attaagatca tcgtattgat cgctcctccg gtaaatgcga ggatcagtgt
      361 atttgacatg gtacccatca tatcttttcc cacatgtatc cctgacttaa acagttcttt
      421 cgccgtcagc tccggtcttt tattatggat ttcttctatt gtcgaggaaa tagaaactgc
      481 aacatccatg actgctccaa gagatgcaat caggataccg gaaaacagta ctccgcctat
      541 ctgaagttta ctgttctgag acacatacac catcgtttcg atatcttcaa tgttataacc
      601 tgtgattctg gttatttttc caaatactgt cgcaaatatg cctgcgatca gtacgcctga
      661 tatcgttccc gtcatagcac agattccttt ttttgtactt ccgcagatca gataaatcga
      721 aacaatcgtc accacgatca ccactgcgac tgctgccagg aagggagaga cacctatgta
      781 catcagcgga atgtataagt aaataatgca gataaaggta aaaacaagtg ccagcataga
      841 tttcagtcca cgttttcctc caaccagaaa taatgccgct cctaaaactg caacgatcag
      901 ccatataaaa ttacctctgt catagccata aatacttcct gacagttcat cgccataact
      961 gctcagctgc acgatcacat tcgttccctt tttgcaataa gttccgtaaa gataaccatt
     1021 catgttgttt gcttcgcata tttttccttt atattttcct gatgtgatct ctactttcac
     1081 attctggctt ccgccattga cttctttgtt tactgtgtta tcctctacaa cctcaacaac
     1141 ttttgctttt acaaaataaa aattatcatc tgaaagcaaa ggaactctgg ttatattgga
     1201 attaaataac caaagtcccg ctgcgaagat tatgaggaaa accacaaata ttgcatttga
     1261 atattttttc attttccgaa tcctctgttt taatatagtt cagttctgca accatttgct
     1321 ttgtggcccg ccgcctgtaa agtcgaaagc cacctcacat cttatgtgtt tcatctgaaa
     1381 attattttgt ttttacttta aatgtaatct tctgctttcc aaaggaagcc gttactttta
     1441 ctttcttttt gctctttttc acagctttca tttttcctgt actggaaatt tttacaactt
     1501 ttttgtttga agttttgtat tttacgttct ttcctgtaac accgttgatg gtcaggctga
     1561 gctgtaccgt tttgcctttc tttaaagcaa tagttgaacc tgcttttact gtcttctttc
     1621 ctgctttcag ctgtaatttt gctgttgcag caggcgttac gttgttgtct gtattgttgc
     1681 cgttattatt gttgttattg tcatttttgt ttgtttcttt tttctgcaga tttacttttg
     1741 cttccagaag actgtcatac tgattttcaa attttttctt tgcaatgagc agctgcatat
     1801 ccatagtctt gtccagtaaa gttgagaatc cggcttttaa agcagtgctg tcgcctcgct
     1861 tataatcacc ctgagcatag ccatagtaat tcagcatatc atttgcatta tctgcatcat
     1921 tcgtcttatc atatacggct gccagctgta ctgcaccttt atcctctgct gtatatttca
     1981 tcaggtcttc ggctgctgca atttcgctct gcagtttatt ccaggatgct tctgtataac
     2041 catcgttttt gatttctttc gcctgtgcga tcagatcctt catggaaacc ttttcgcctg
     2101 ttttgtaaag tgtataatca tctgcatatt tgtttccatt gtaatcgtat gttttgataa
     2161 cgatggattc atccgaaaaa tcaatggttg agaatgtcgg aatctgtgtg ttggaacgct
     2221 ctgcgatgta atactgtttt gtggttgcca gtttatagaa cttacttcct gatgcagaac
     2281 cagccgcaat gtaaagtgtt ccttccggat tgatcgctac agaatcatca tactggatcg
     2341 ctgttccgtc tgccatcaga taggatcttg cataagaatg atcatgtccg gttaagcaca
     2401 tatcaatacc gaactcatcc atcagcggtg caaacagtgc tcttaagttt gctccgtctg
     2461 tgtcagagtg cggctgaccg gaaccataga tgtcgtgatg gaacataaca actttccatt
     2521 tcgcatccgg attgctttcc actgcttttt tcagaagttc tctgtgttca acggtatttc
     2581 tgttgttgct gttcagactg ataaacagta cgtttccata gctgaagtaa tagtcagaac
     2641 cagagtttgt gcttccaagt ccgtcttctg aattcggatt attgtagtga tatttataat
     2701 cggttccttt gctctcgtga ttaccaatgg tcgttgccag cgggagcatt ctcaaaagtg
     2761 ccggataagt aaatcctgca tattcgcttt ctctgacatt ctttccatct gaactgtctg
     2821 ttcctgcata gtcaatctgg tctcctgccg acagaataaa gcttgcattt ggtgctgtga
     2881 tctttgcctg ttccagggtt ttattccagt taaaggtatc tactgcaata ttgatatcat
     2941 cggcagttcc ctgaccctgt gagccggatg ctccgatctg cggatcacct acgagaattg
     3001 tctgaaagtt tgttgtcttt tttgttgtat aggactgaac ttcactccag tttggatttt
     3061 ttacatcatc ggtataactg taataatagg ttgtattttc ttcaaaaagt ccttcaaccg
     3121 taactttatt agatgcttta tatgtatttt tctgattcga acgattgatc tccgttgccg
     3181 ttcctttaaa ttcctgtgca tcgctcaaat cttctttttt tccgattttt actgccggct
     3241 cgcccttttt ctggctgtac catgcaaatc ccagatcttt tgctgtctta ccaggtgtta
     3301 agataattct tgatgaatca taggattcac cttctccggt aagatttccc tgatcgtctt
     3361 ttttattcca gacattatct ttccaggtgg cataatctga aatattctgt gtatctgtaa
     3421 ataatgtgct gcttgcactt gtctctccat atgcaaaaac attgaccggt gctgatgttg
     3481 ctgctactgt tactgccatt gctgcagcga tgatctgcaa agctttcatt ttcttcatat
     3541 tttttattct cctctgcctt cacaggctct ttttaaatgc attttgattc ttttattgat
     3601 aagtcttatc atttggcagt atacaggtgt tatgtaaaga cagtttgcgg atatgtgtaa
     3661 aatctaagta aatttaactt tctttttttc ccgcagctct ttaaaaaatt cactcagcat
     3721 ggtgctgcat tcttcctgaa gaactccttt tgtcagttcc acctgatgat tgaagcgatc
     3781 catctgcaaa atatttaaaa cggaaccggc acagccagcc ttcggattca tgcttccgat
     3841 gactacccta gatactctcg cctgaacgat tgccccggca cacatctggc atggttctaa
     3901 cgtaatgtac atcgtacatc cttccagtct ccagtcacca agttttttgc ttgctttcct
     3961 gatcgcattg agttctgcat gggaaagtgt atttttatcg gtgtttctgc gattgtaacc
     4021 acgtgcgata attttatctt cataaacgat cacgcagcca atcggaactt catccagtgt
     4081 ttttgctttt ttcgcctgtc ggatggcttc cttcatatat ttttcgtctg ttgtcatatt
     4141 tttcttttgc ctttctgtaa atgtggtata ctgaaaatat cattttttat acaaattcaa
     4201 gtcgaacgca cgtgagactt agcatgcgat tccggtcatc tctggctaat atttgcctga
     4261 cagaatcgct gtgtatacat tgattttttg caattttaat tccaggaggt ttttatgaat
     4321 attcatggtt ttatcacttt aactctatta gattacccgg agcatctcgc tgcttctgtt
     4381 tttctgggca gctgcaacct gcgatgccct ttctgtcaga atggaaacct tgtcctttcg
     4441 ccggaaaatg aaccttccat ctccgaagaa gaagtgcttg cctttctgaa aaaaagaagc
     4501 ggtattctcg aaggagtctg tatcagcggc ggtgaaccta cgctgtatcg cgaacttccg
     4561 gattttatat caaagatcaa agctcttgga tatcttgtga aattggatac aaacggaaca
     4621 aatccggaaa tgcttcgttt tctttatgaa aatcatcttc ttgattatat tgccatggac
     4681 atcaaaacgc aaccggaaca atatgcaaaa gtttccgggc tgcagccgga tgctgcaggc
     4741 acacagaaac ttctgcaaaa tatcaatctt tcaaaagaat atattatgtc ctgcggcata
     4801 gattatgaat ttcgcacgac cgttgtggcg gagcttcacc gtgatgagga ttttaaggaa
     4861 ctcgggaaat ggctgtccgg atgccgccgc tattatctgc agccttaccg ggaatcagaa
     4921 cacattcttg cttccatgag aaatccagat gtctgttttc acgctcctga tacagaatct
     4981 ctgcatcgtt atattgaaat tcttaaaaag acaattccaa atgtggaatt gcgcggaaaa
     5041 gatatcaagt aaataatttt gggcacatct aaattccgtt ttactgtctc aacattaagt
     5101 gctttaatct gtttttcatt atacaaaaat gcctgtgaca ttttataatc caaaaacgtc
     5161 acaggcactt atttttattc cgagcatatt tcttttattt aatttttaca ctctttggta
     5221 atccgcttgc tcgaagcaat tttgtataaa gtttcttttt tgatttcgga actttcacaa
     5281 cagcattctt tgaaatacct ttgaaagcat tgcttccgat ctttttcagg ttcttgcttt
     5341 taattataat tttcttcaga tttttacagt tttgaaatgc acttttacca atcttttccg
     5401 ttgtttttcc aacaaccaaa gttcttacct tcttatttcc tttcagtgca ttttttgcta
     5461 cggatgtaac cttatatttt tttccattca gttttactgt actgaaaatt ctcaggttct
     5521 tctttttttc accattcact gtactggtaa tcatctttgc agtatttttt ccggttatgc
     5581 ggtatgtaaa aatacctatc ttattctgat ccggaacctg agacggtgtc tccggcgtct
     5641 gtgattcctc tgtctcaacc ggcttttttt ccagtgctgc gattgcatcc tcgattgctt
     5701 ttgccatctg gtctacttcc gcctgctccg tgatattctt tccacgtatc actttatcga
     5761 ttgcttcctg tactgctgaa aagtctttgt aatcgtcctt cttcaacgca tttgcttttt
     5821 cgatcgcttc atccactttc ttataatctg cgtctttgta cgtcagagta aatgtttgtg
     5881 tatgggttgc tgtatcagct gtaatggtaa tttctgtatt taaagtctca cagttgtcag
     5941 cagtgatttc caccggataa gtgcctgctt caagttcagc aaaatttgcc gtttcctgtc
     6001 catttacttt tatgaccgca cctgtcagtc cagatggagt cacagtaaag aaaacggaat
     6061 attttacatt cggcaaaagc ggtgtttcac cctgactgaa acgatagata ccgccagcag
     6121 cactgatttc attatagaaa tcttctgttt tcatatactc gatcgttttc gctgtacctg
     6181 ccccatcttt atttgcacag gcagatacat tttccgtctc ggcaaagaaa ttattttcaa
     6241 aagttgccag agctttatcg gaactgtctt tttttcctgc gattgcacca aaacgtttat
     6301 acggaagtgt ggcagtatac tgcgtcagat caatttcgcc gccaaaatag cagtggctga
     6361 ctgtattatc actgccgtcc tttgaattcg tgccaaccac accaacaata ccgcccatat
     6421 ccgtgattcc ttttccaagt ggtgtcagtt taccggttga acagcaattt ataatttttg
     6481 cagttccgct gatctgacct gcgatccctc cagtttgagg agcgtaagac tttatatctc
     6541 cggaattaga acagtaacgt atttccgaag ttcccatgac cattcctgca attccaccta
     6601 cgtaaaaact gctcacatca ttggttattg tgatattacc tttattttga caatattcta
     6661 ttactgtatt ttctgcatat ccacacaacc cgaaagttcc gttgatatac ttgtcatgat
     6721 tattgaacac tacttccgat atacaatcag aaatttttgc accatccgta tatccggcaa
     6781 gtgtacctaa aaatacatat ccttccccgg catcatcaat attccctttg agcgtcagtc
     6841 cggaaatttc cgccccccca aggaatccga ataatcctcc aatatcctct tttccataca
     6901 ttgaagcata atcaatattt gaaatactat gaccgtttcc ataaaattta ccactgaaat
     6961 aatgttcaac atctccttcg tcattggtac atccgatcgg cgtccactca acacctgtca
     7021 gatccagatc tgcttcaaga gataccaccg catttgtcct gttatcgtac ttaccggcat
     7081 ttacatcttt tgcaaattgc aaaagttcgt caacggctgt gataactgtc ttgctttcct
     7141 gcccgttttc ttctgccagt gctgtcgctg gaacaagcga catcacaagg cacaatgtaa
     7201 gcataaaact cagtaattgt tttttcatac tctttttcct cctgtcagat aatgtcaaaa
     7261 tttacatttg ctgattttat gatgccaggg tcaaaaggtc taaatccaca tgagcttgct
     7321 cataggtgga tgaaagacct tgggacccgc tccgatatga gcataaaagt gggatgataa
     7381 tcccacgata tgcgaatact ggtgaggatt gtggaagtgc aacgcacgga acaatccgca
     7441 ggcatcatag ttggggattt ttgaccccat catcatttta ttataacaga aaacattttt
     7501 aaatggaact agccaaacgg ttagtatgtt gcggtctaca atatttttac aaaaacattt
     7561 atatcagatt tttgcaccag taaccaaagg tctgttcttt tatttccgat gtccgctctg
     7621 tttttgccct gcgaaactcc ggaaatccaa acatttctgc catatttttt tcgccataaa
     7681 atccagggat tccaagaata tagcctccct ctttaccttt tcctatcatc agatgctgat
     7741 gctgataata tccatgcatc agaaaactgt tgcttccctg ccgcagacct ttctgccaga
     7801 gaatttttaa gtccgatggc tgaatgcgaa ttgcatcttc tatcttttca tcctgcaccg
     7861 gctgaaaatg tatataacgt gctgtggcac gattccatct ggtatccgtt cccggctcgc
     7921 agccctgaca tttgttttct gtttctgcac ttctgatttc gttctcgttt tcagattttc
     7981 ccgcagtttc tttttgtgat atttcttcat ctgtttcttt tggtctttct ttctgtgata
     8041 ctgcatcctg taattcctgc gatatttttt ctgatacttc tttttgaaat gtttcttccg
     8101 atatttttcc tgatctatct ttcaatgata ctttttctgg tatttcttct gacacttctt
     8161 ttgagaatat ttcttcctgt atctcttctg ctgtgatttc cggctccgct ttcgcatgtt
     8221 ccgttatatc cagatttata ttttcttcct gcaattcctg atcagcttct gactctgtag
     8281 atttttcact tttttcgttt tccttaatcc caacattttc tttatctgtt tcattttctt
     8341 caacggcttc tttatccgtt tcactctttt tgacattttc ttcgtctgtt tctgccttta
     8401 tttcttctgg tttcccaaga ttttcgatcg caattggctc atcatcgaaa acggtcatcc
     8461 agtttctacc gctctgactt ctgatataga ttccacagac ttgttcaaaa cgacaggatt
     8521 cttccacaaa cggttcttct ggaacggtaa aacgccggtc aaatccactg ccctgtgtct
     8581 gcccttctcc gatcaggaga ataataggat tgttttttct gctctctttt ggctgtcgca
     8641 caattccgaa tacttctgtt tttccatcca gcattcttat attttgaata tgtatctgga
     8701 tgcggcaaat ggcatttcgc agttctattt ttgcaaaccc tgtattctgc atcttctttc
     8761 catttacata ttcataaata taagccacaa aacgatgata atctgccatc catctcgtcc
     8821 tccacagacc tcgattttct ggtgtgatta tatgctcgtt ttgtggctca tatgcaaggc
     8881 agatatttct gccctctgta tcacagaact gtaaccattt tttccggttc ttttatattc
     8941 ttgctgctcc tgtttctctt gcaacttttg caactgccgc agatactgct tcttttactc
     9001 tcggatcaaa tggatccgga ataatgtaat ctgccctcag ttcactttca tcgatcaggt
     9061 tcgcgatcgc atgtgctgcc gctgcgttca tcggatcatt gatatctttt gcacgtacat
     9121 ccagtgcacc gcggaagatt cctgggaatg ccagtacgtt attgatctga ttcgggaagt
     9181 cactgcgtcc ggtaccgact acggctgctc ctgccttcat tgccagatcc ggcatgattt
     9241 ccggaaccgg atttgccata gggaaaagga tcggatcttt tgccatggtc tttaccattt
     9301 cttccgttac ggttcccggt gcggatactc cgataaatac atctgcacct ttcagtacgt
     9361 cagcgaggct tcctgcctgt ttttctctgt tggtgatcgc tgccatcttt gctttttctt
     9421 cattcagacc ttctctgcct tcatagatgg cacccttgcg gtcgcacatg ataacattct
     9481 tcaatccaag ctcgatcagc agtttgatga tcgcacttcc ggctgctcct gcaccggaca
     9541 taactacttt aatatcttcg atttttcttc cggtaaactt cagtgcattc agcagtgctg
     9601 ctgctgttac aacggccgtt ccatgctggt catcatgaaa taccggaatg tcgcagcgtt
     9661 cttttagttt tctttcaatt tcaaagcatc tcggtgcaga aatatcttcc agattgatgc
     9721 ctccgaaact tccggcaaga agtgccaccg tattgacgat ctcgtctaca tctttgctgc
     9781 gaatgcaaag tggaaaagca tccacatcgc caaattcttt aaacaaagca cactttcctt
     9841 ccataaccgg catacctgct tccggtccga tatctccaag tccgagtact gctgtgccgt
     9901 ccgttacgac agccaccagg tttcctcttc ttgtatactt ataagacaaa tcaacgtctt
     9961 ttgcaatctt caggcaaggt tctgcaactc ccggtgtata ggccactgcc agatcttccg
    10021 gtgtctcaat ttttgctctg ctgatcactt cgatttttcc attccatttt tcatgctgat
    10081 ttaaagcaaa ttctttttta tccattttta ttttcctttc tttttcggat acaatttgca
    10141 tagtaattct gtactcttat cattaccaga tgactgtctg tttacaacca tctttatcaa
    10201 atctgcatga acacctgacg ttttcttttc tcacccgtaa ctgttctgta tctttacagc
    10261 tactttcacc ctttaataac ctgttacacg aaatcctttt tcacctggct gcagcggcat
    10321 acgccttgct tctatgtctg tgatccgtat ggatctggct tcatagaggc gggcaaggag
    10381 gcggtctata tcagaatccc gtccctgtac ttccatctcg acccttccgt cacaaaggtt
    10441 gcacacccat ccggtcagtc cgagatcatc ggcagcatac ttcgcctgat aacgaaatcc
    10501 taccatctgc acatctccgg aaaaataaaa atgttttctt actttatccc tcataggctt
    10561 tctcctttgc ctctgcctga tttttcattg caccaaaaca gttataattt atgccttaat
    10621 attttccagc acttttacca gataatccca catacgtgcg gtagaagaaa tggaaagaac
    10681 ttcttctgtt gtatggatat ccttcatggt cggtccaaag gaaactgcat ccagaccttc
    10741 gatcttctca tagaaaagac cacattccag acctgcatgg atcgctttta cttccggtcc
    10801 gtctccgaac agctctttgt aagaatctac cataatgtca cgcagtttgg attctttgcg
    10861 gtactcccat gccgggtagt cgccctctgt tacacagtct ccgcccagga attcgctgag
    10921 gtagcaaacc tttgcagtca gtgcatcttt tgcactgtca atgctgcttc gcacactgct
    10981 gacaattttg cattcttctt cgtttagcat cagaatacca gggttgatag atgtctctac
    11041 cagtccttcg atggaaccgc tcattttctg gatgccatat ggcacctgta ccaggaagaa
    11101 aataatcttt tcctgactta cctgctccag agctttttcg gttgtctcac cgattttttt
    11161 cacagtcacg gtaatgcctt catcggttcc ggtatattcc gttctccagt cattctgaag
    11221 tttctctgct gcagcaaaaa ttgcttcttc tgcttcttta tctgcaacaa aaacagcttt
    11281 tgacagtctc gggatcgcat tgtcctttgt gccgccttcc agtgctgtca ggcagaacgg
    11341 gatatcctgt tccagtgtaa ataaagcctg tccgatcagt ttgttggagt ttgctctgtt
    11401 tttatcaatc tctgctcctg aatgtccgcc gttcagtccg gaaataacca gctcatattt
    11461 ttctccgctt acctcctgat atctgaccgg aatcgttgtg attgcagaaa gtccgcctgc
    11521 acagcttacc cacagatatc cttcttcttc ggaatccatg ttgatcaggt atttaccttt
    11581 cagctgtgag cagtccagag cttttgctcc gagaagtcct acttcctcat ctgttgtgat
    11641 aaccacttcc agtgccggat gtttcaatgt atcatcatca aggattgcca gtgcatatgc
    11701 aactgcaatt ccgtcgtctc cgccaagtgt tgttccgttc gcagtaacat aatcgccttc
    11761 gatcatcaga tccaggccat ctttttcaaa gtcgtgtgtg gaatctgctg ttttttcgca
    11821 gaccatatca cagtgtccct gcaaaataac gactggtgca tcttcgtatc ctgcggatgc
    11881 tgctttgcgg atcacaacat tgttgctgtc atcctgactg cactccaggt tacgttcttt
    11941 tgcaaactgt accagataat cactaatctg ttttgtatga taggaaccat gtggaatttt
    12001 acaaagctcc tcaaaataat aaaatacttt ttccggtttt aaaccactta aaacacccat
    12061 tttcatttcc tccagacatt tttattgtcg tccgaatcca gacagcactt ccattgtata
    12121 attaaatatt ttctgatacg tttccgcatc aaagtgtacg ccgtcctgtg tggaatatcc
    12181 actctccagc agataggaat ttgtctctat ccaaccagtc tctgacggaa gattattata
    12241 aagaatattg ttaaaattcg caatatcctc attggaaata tacggatgat tttctaccgg
    12301 atttaccgac atatagtaga cttcggctcc tttttccatc cactgaggaa ttttctcttt
    12361 cagaagatca atatatcccc atgcatttcc aagatcgttg actcccatat tgataatgat
    12421 cacagatccc tgcgtcactg actcatcaat ctctccaacg gcagtatcct gaaaccagga
    12481 taatcccgct gaaacctgac cgatccacac gcagccggaa tctccgactg tttcatgcat
    12541 gccgaccgtc ctggaatctc cgacaaaaat atactgcgtc acatcaaacg atgtctcgct
    12601 gctctgctct tccgtttgtt gttctgtttc cacttgctgc gtctccgtct gttcttcttt
    12661 ttccgacatt atttgtgttg ctttgcttac tgcaacagca ttggtacgga tacagctgaa
    12721 attgtattct tccagacaat cccacgccat atccagcagc acttctcttg tcggtgtacc
    12781 aagcggtgcc ttcgccccgg ctgtaaatgc cggaataaaa aggcagcaca gaagcagaat
    12841 ccatctgcta aactttcttt tcttcatgat cacatcattc cagttcaaat gctcctgtat
    12901 aaagctgata atatttgcct ttttcggcga tcagcttatc gtgagatcct ctctcaataa
    12961 tacgtccctg ctccagcacc attataacat cagaatttcg tactgtcgac agtctgtgtg
    13021 caataacaaa tacggtacgt cctttcatca gagcatccat acctctctgt acgattgcct
    13081 ctgttctggt atcaatcgaa gacgtagcct catccaggat cataacaggc ggatcagcta
    13141 ccgctgctct tgcgattgcg atcagctgac gctgtccctg tgaaaggctt ccgccatttc
    13201 ctgtgatcac cgtatcgtaa ccattcggaa gcaatcggat aaagtcatcc gcatttgcaa
    13261 gttttgctgc tgcatagact tcctcatccg ttgcatccag ttttccgtaa cggatgtttt
    13321 ccatgatcgt tccggtaaac aagtttgttt cctgcagaac cacaccaaga gaacgtctca
    13381 ggtctgcctt tttgatcttg ttgatattga tgtcatcgta acggattttt ccgtcagcca
    13441 gatcatagaa acgattgatc aggtttgtga tcgttgtttt tccggctccg gtagaaccta
    13501 caaacgccac tttttgtccc ggttctgcat acagactgat attgtggagc acgatctttt
    13561 cttcattata accaaaatct acatcataga accgcacttc gccacgcatt tttgtgtaag
    13621 tcagcgttcc atcgccatgc ggatgtttcc atgcccacag tccggttctt tcttttgatt
    13681 ccgtcagctc accgttctcg tcatattttg cattgaccag tgtaacgtat ccttcgtcaa
    13741 cttccggctc ttcatccatc agtttaaaaa tacgttctgc tccggcaagt gccatgacaa
    13801 ccatattcag ctgatttgaa atctgactga tcggattcat aaagcttctg ctcagctgca
    13861 ggaaagatgc aatcgtacca accgtcatac cgcctgcacc gccaagagcc attgtgcctc
    13921 cgattgttgc cagcagcaca tactgcaggt ttccgagatt acccatgatc ggcatcagga
    13981 tatttgcgaa actgtttgct gcggatgcat ccttgcataa ttcttcgttt ttctggtcga
    14041 aaatctcttt tgctttctct tcgtgacaga atactttgat aactttctgt ccgttgatca
    14101 tctcttcgat ataaccattt acatcaccaa gagctttctg ctgacgtata aaataataac
    14161 cactctttcc ggcaacacgg cctgtcactt gaagcatgat aaatacaaag cagagtacaa
    14221 aaatggtaag ctgccagctt gtgaaaagca tggcaaaaaa cacagaaata attgtgatca
    14281 gagaggagaa catctgcggg atactctgtg caagcatctg acgcagcgtg tccgtatcgt
    14341 tcgtataatg gctcataata tcgccatgtg tatgcgtatc aaaataacgg atcggcagtg
    14401 tctgcatatg ttcgaacatc tcgtctcgaa tacgttttaa cgttccctgt gcgatactta
    14461 ccatcgttct gttgtaaaac aggcaggcaa ggattcccac tgcgtaaata catcccatga
    14521 tcaaaatgga acggaacagc ggtgcaaagt ccggtgtggc tgcctgaagc agcggtgtga
    14581 tataactgtc gatcagtgtc tgcagataca gtgagcagga caccatggca agtgcattga
    14641 ccacgataca gatcaggaca gcagcaaaac gcagtttata atgtccgata taagacatca
    14701 gtctttttac aacatcttta tttacttttg ccattccttc cggtcctttt ttgttattcg
    14761 gctgtctcat cttcgcttcc tcctttcacc tgagaatagt aaacctcctg atagatctca
    14821 ttggatgcaa gcagctcttc atgcgtgcca accgcagaaa tatggccttt atccataaca
    14881 ataatcttgt ccgcatcctg taccgatgaa atacgctgtg cgatgataaa tttcgttgta
    14941 tccgggattt cctcacggaa tgcctttcgg atcaacgcat ctgttttcgt atctaccgca
    15001 ctggtagagt catccagaat caggattttc ggttttttca gaagtgctct tgcaatacag
    15061 attctctgtt tctgaccgcc ggatacattg cttccgccct gctcaatgta agtatcgtat
    15121 ttatccggga atgtctggat aaaatcgtca gcctgggcga gctgacagac atgaatcaac
    15181 tgttcatctg tcgcattttc atttccccag cgtaagtttt ctttgatcgt tccggagaac
    15241 agcacatttt tctgaagaac catcgctacc tgatcacgca gagcttccag atcatatttt
    15301 cttgtgtcca gaccgcctac tttcaggctt ccttctgtga catcataaag tctcgggatc
    15361 agctgaacca gcgttgtctt tgccgaaccg gttcctccga tgataccaac cgtctcaccg
    15421 gatctgatat ttaaattaat atcagaaaga cagatcttat cctttttccc tgtgtagctg
    15481 aagcttacat gatcaaatac aacggaaccg tcttttactt ctgttaccgg agattctcca
    15541 tttgtaaggt cgcttttctc attaagaatc tctacaatac gctctgcaga tgctctggaa
    15601 ataatgatca taacaaatac catcgacagc atcataagac tcatcagaat ctgcatcgca
    15661 taagaaacaa ggctcatcag ctgaccggta gacataccat ttaccggatc atttccggtt
    15721 gtaacgatca gttttgcacc aaaccaggaa atcagaatca tacatgcata catgcagaac
    15781 tgcatcagcg gcatattgaa tgcgatcagt ttttctgcaa atgtaaaatc gttataaatc
    15841 gatgtggaaa ttttttcaaa tttctcaatc tcatgatcct cacggacata agatttcaca
    15901 acacggattc cgtgaagatt ttcctgcaca acattgttca gtttatcgta agttttgaaa
    15961 actttttcaa atactggatg tgcatgtgtc ataataaaat acagtccgat gccaagaatc
    16021 ggtgccgccg ccaggaaaat cactgccatc tcagaattta cctgaaatgc cataaacata
    16081 gaaaaaacaa gcatgatcgg tgcacggact gccatacgga tgatcatctg atatgcattc
    16141 tgcacgttgg taacgtccgt tgtcagacgc gttacgatac ttgccgttga aaatttatca
    16201 atattggaga aagaaaaatt ctgtacattg taatacatat cctttctcag gttctttgca
    16261 aatccggcag atgcccgtgc ggcaaattta ccggaaagca caccgaacat cagtgagatc
    16321 atcgctgtga cagcaagcag aatacccatc ttccagatat atcccaggtt ctgtgcctga
    16381 ataccgtagt cgatcagtct tgccatcaac atcggaatga tcacttccat gattacttca
    16441 agcgtgacca tcgccggaga taaaagggaa tccttcttgt actcacggat gctcttcatc
    16501 agtttcttaa tcatgattgg cctccttatt atcttttttt tcgtaacatt tgttttttat
    16561 cgaacattcc agatcttcta tgaggttctc gcgaatcttc tgtgtcagct gaaacagaca
    16621 gtcaatatcg tccgctgtca tatcctttac caacagccgt tccattctac ggatttgctc
    16681 tttcacaaga tcactggctt catcgccctt ttccgtcaga acaattttct tcagtctcgc
    16741 atccccggga taagactgca tttcaatata tccattctgc tccattttct gcaaaatacc
    16801 ggcaacagta gagcgtgtaa ggcagaaacg cttcacaata tcccgcggaa aaatgtcctt
    16861 ttgaggattt tctttaatat actttataat cccgccctgg acatactgga gatcataaat
    16921 tccacttttt ttcttatcgt actcaatttt tcgatataaa agattggata atgacaagat
    16981 ctctctgccc acttcacgtt tttctccaaa atccatcttc gcttcacctc ctgtctgatt
    17041 gttcgttgtc gaacaattat aaggcgaata aaattttttg tcaatcgtta tatcgcacca
    17101 attttttcat ctgaaatcaa atatttttgg aagcatactt tacatctttg ccgcaaaata
    17161 tttttggata ccgtcacaaa tcgattctgc tgccttctgc tgataggtat ctgtaattaa
    17221 aagtgctgcc tccctctcat tcgacagaaa accgcattcc acgatcaccg ccggagcttc
    17281 actccgtttc aatatgtagt acgtgtcatt cgccttgatt tcccgcggtc tttcgatctg
    17341 cagctcttta ttcataacat tctgaataca tccggcgatc tccttgccct ctgccgaagt
    17401 cgtataataa aaaacctgag gtcccgatac atactcctcc gtgtaactgt tctgatgaat
    17461 actgaccgcc atcgccggtt ttgtctccct gatctttgca caccggttcc tcatatccgt
    17521 gctcttttta tttccctgct ccggtccctt tacactttcg tccgcctccc gcgtcatcac
    17581 aacctcaaat cccgccttct ccagacaaac ttttagcttc tttgcaatcg ccagattgat
    17641 atccttttcc ttcgcctgat tgattccaat cttccccgga tcatcaccac catgccccgc
    17701 atcaatcaaa ataaccttct tctccacact gccggaagcc ggaagtacat tttttactcc
    17761 gcccgtaaaa agcagaattg tacacgccag caccagaatt cccatccata cttccgcagg
    17821 gatatttttc agccgcagat catacatcaa aaccgtcacc catttttgga ctcttgtgac
    17881 agtttattca gaaagaaacc catttaagaa caggttaaat gcaaatcata attacctggc
    17941 actgttagga cgccactttt tcagtggcgt cctgttagaa gtgggagtct tctccgacta
    18001 agataaaaaa tctctgcaag agttcctgcc gtaagtcgct cctgcagagg ggaaatgttc
    18061 atttaatcca gtgcctgttc aaactcctga acggtatttg cctttaatgc ttttgtaaac
    18121 caaatgtcca aaagatctac atccggttct tcttcgattc ttttcctcgt cttttcatcc
    18181 acgcctcctt tcatttccag cacacgtaaa ataaattcca ctctgctggc tttcttttcc
    18241 tcttcccttc caagctcaat tcctttcgca atgcccatct tttccagcct ctctgccaca
    18301 tcacacatac tatgcacctc cttctttttc ttgaacattt ctttataacg gtcgtcacct
    18361 gtcattacct gcaacagttt taacacttca tctacatgct ttatttctgt cggatcatcc
    18421 gggatataat ctttgttctt tctttttttc acaaagaaat ttgctacgat tcgaaaatca
    18481 ctttggaatc tttctatttc ttcctctgta agccatgcga tctcaaagac ctgcatactg
    18541 tagtcattta caaacttatc cagcccttcc ggtatttcca tcaaagattt aatatttttc
    18601 ttgctgttcc agtgtctgtc cgttccaaag tataatacga ttgttaccac cggaagaatc
    18661 ttttttctct cttccaataa ctgactgcgg tacgctgtcc cgtcatatcc tatcacgcgg
    18721 aacggcatat ttttttccac aacactctgg ttctcaatcc cgcaaatcgc aagctctact
    18781 tccttttctt tccagtactt ggctacgtct cgctccagct catgtacctt ttcatcttcc
    18841 gccttgtact gtgaatgaat ggacgtattt tccagtgcct ccggcaggat tctttgttct
    18901 cctgcaaaaa gcagtccatt cataatatcc gcaaatacat cattataatc tgccagaatc
    18961 ttttctgtaa tatccttttc tcccatagct tctccttttc ttttgttctt tttacgtgaa
    19021 gaagccacag gatgtaagtt tttccctcaa ataacatttc aggtaatact gcttactttt
    19081 aagtatacga aaaattttta tttcttcaag ccgcttcacg caggtctttt ttaaattgga
    19141 ttttcctgcc gaacggcatt gaattttcat tgagttttga aaagcaatac gatttttgct
    19201 ttctgtttga tagatatata atatatttta cttctgtttt gtgtttatta taattatcta
    19261 tgtgatgttt gtcaactatt ttgttatgtg ctttgcatgg tatttttaca tacactttat
    19321 aaaatcaaca tcaaaagtaa actttataac aatagggagg caaccctccc catcaaaaaa
    19381 tttcatcttt tatccttctt ttaattttct gatcagctta cgatattccg gacgttcaaa
    19441 gtcttttccg gactgataat cgcagtaaat attttttggt gaaatactat atggttttaa
    19501 tgccatgatc tgcctgtcta tgtgctgctc ttttgtagaa actctgatat atgcatattg
    19561 ttccatcttc atcctcctgt gctatgattt ttctcttaaa taatacaagc gctgccgcga
    19621 agatgagatg ccccttcgct tcataaatat tgcaaataaa tttatatcta tatcagtaat
    19681 atattttata caatatgtaa ctctctattt tatttttaaa ttttctataa acttttcaaa
    19741 atctcttgtc aaatatttca atcaatgtta ctatatcttc aaagcatatt tctctttacg
    19801 gtatttctac catctgtttt ttctaatctc atttcagaaa tccatgcttt tatcccatca
    19861 aaaaaataaa aagcaggagt tctgaataaa aaacagaaac agacttttta tctgtctttt
    19921 atctcctgca atacaggagg taatgcctat ggcaagaaaa aagacagctg ctacgaagaa
    19981 aaagcaggct ggaaaattgc acacatctgc aaaccgcaaa tataaagaca cggtatttcg
    20041 tatgctattt gcagataaat ccaatctttt gtcactctat aatgcaatga gcggaagcga
    20101 ttatcacgat ccttccaaac tgcagattat tacactggaa aatgcgattt atataggtat
    20161 gaaaaacgat ctggcatttg ttatcgacac aaatctgttt ttatacgaac atcagtcaac
    20221 ctataatcca aatatgccgc ttcgcaactt gttctatatt tccagtgaat accagaaaat
    20281 ggtggattca aacaaactct attcttcgac actgttagag attcctgcac cgcattttgt
    20341 tgttttctat aatggaacca caaagcagga agacagctgg tacaactacc tgtcagactc
    20401 ctatcataat ttttccggta ttcccgatct ggaactgaaa gtagtgacct taaatattaa
    20461 taaaggacat aataaagaat tgatggaaca gtgtcagacc ttaaacgaat atgctcaata
    20521 tgtagagaaa gtccgtactt atacaaaaga agacaactta gataacgctg tggaacgtgc
    20581 cgtaaatgaa tgtatccgtg atggaattct cgcagatttt ctcgcccaaa acaaatcgga
    20641 ggtaattgct atgagtattt ttgaatatga cgaaaaagca gttcgacaag tatactatga
    20701 agatggactt aaagctggca tacgaaccgg cattttaaat atgctgaaag caaaaaagta
    20761 ttccctggaa gagatttctg aagtttttca ggttccggta gaggagattg agcaaataaa
    20821 aaaacaacaa atataatttc cctattgaca aatccccacc ccggttttat actcagaaac
    20881 aacaaaatat ttatcacttt aaaccgatga acaagagaag taagcaggaa gttttattcc
    20941 cagagagctg caggcggtgt gattgcagta ataaagattt tgccgaatgg acttgtgagg
    21001 gcagtctgaa actgtatatt ttctttatca agatataagg agtaggaact gacggtggct
    21061 cgatccgtta tcagcagagt atgtatgaat gtacataaca gagtggctgc aatgcagcaa
    21121 tttgggtggt accgcagaag taatttatag cttttgtccc tttagagggg acagaagctt
    21181 tttttattat gtcagggtca taggtctgaa accacgatat gcaaatattg gagcaaattg
    21241 tgagagtacg aaatacgaag caattcgtat ggcattttta tatatttata aaaggaaagg
    21301 agtatcgcat gtatccagat tgtgatgaga ttttaaaact tgcaaatgat tacgatatta
    21361 ttccggtctg ccgtgaaatt tatgcagatg ttatcacacc gatcacgctt cttagaaagc
    21421 tgtccggcat cagcagcaga tattacctgc tggaaagtat tgagggcggc gaaaaatggg
    21481 gacgctattc ttttctcgga tacgatccgg tgatgcgtgt ttcctgcaag gagggcgtgg
    21541 taaccatcga aaagggcgat gaggttgcaa caaagcagac agacaaaccg ctcgatgttc
    21601 tgcgtgaaat catgaaggat tacaaagctc cacgtcttgc aggactccct ccttttgccg
    21661 gaggatttgt cggatatttc gcatatgcca tgatcggata tgcggaaccg acactgaaca
    21721 tcaaaaaggg catgttcaat gattatgata tgatgctgtt tgataaggtc attgcctacg
    21781 atcatctgac tcagaagatc agcgtcattg taaatatgaa gaccgaccgt gtgatggaga
    21841 attacggaaa agctgctgcc gatatccaaa atatcatcaa nnnnnnnnnn aatcccggtt
    21901 tcttcttcaa aagtgaattt tacctgcaat gtcactcagg aagaatactg taagctggtt
    21961 gaaaaaacaa aagaatacat catcgatggt gatattttcc aggcagttat ttcaagacag
    22021 ttctccactc cttacgaggg cagcctgatc aacccgtacc gtgtactgcg taccacaaat
    22081 ccttctccgt acatggtgta cctgagcatt gacggtgaag agatcatgag tacttccccg
    22141 gaaacgctgg tccgtctgga aaatggccgc ctgactacat tcccggtagc cggttccaga
    22201 ccgagaggca caacaccgga ggaggacaag gttctggaaa aggatctgct ttccgacgaa
    22261 aaagaacttt ccgaacacaa tatgctcgtc gatctcggca gaaatgatct cggtaaaatt
    22321 tcgaattttg attcggttga ggttacaaaa tacatgatga tccaccgcta ttccaagatc
    22381 atgcacatct gctcacaggt tgagagcaat atccgcagcg actgcgacgg cttaagtgcg
    22441 attgaaagtg tcctgccggc cggaacgctt tccggtgcac caaagatccg tgcatgtgag
    22501 atcatcgaag aactggaaag tgaacagcgt gggatttacg gcggtgcact cggttatctt
    22561 gattttaccg gaaacttgga tacttgtatc gcgatccgca tggcagtcaa aaaagatggt
    22621 catgtgtatg ttcaggcggg aggcggtatt gtcgctgaca gtattccgga aaatgaatat
    22681 atggaatcct acaacaaagc actcgctgtt atgaatgccg tgaaaaatgc aaaggaggtg
    22741 ctggaataat gattctctta attgataatt atgatagttt ttcttataat cttgttcaaa
    22801 tggcaggaag tatcaacccg gatatcaaag tcatccgaaa tgatgccatg accgtagaag
    22861 aaatccgtgc cctcgcacct tctcacatca ttctgtcccc gggacctggt tatccgaaag
    22921 atgccggtgt ctgcgaagag gttgtgcggc atcttcaggg agaaatcccg atcctcggcg
    22981 tgtgtctcgg acatcagggc atctgcgaag tatacggctg caccatttct catgcaaaac
    23041 agctgatgca cggaaaaaag agccgcatcc acatcgataa caacagtccg atcttcaaag
    23101 gactggacga agaaatggat gccgccagat atcattcact ggttgctgtc cgtgaaaccg
    23161 ttccgaatgt tcttcaagtg atcggtgaag ataaaaatgg cgaagtaatg gcagtacagc
    23221 ataaaacgta cccggtatat ggtctgcagt tccatccgga atccattctc acaccgcagg
    23281 gacgcatcat tcttgaaaac tttttaaatt tatgatgcct acggattatt ccgtgcgtcg
    23341 cactcccaca atatacagga ggaaatttac catgataaaa gaagctattt acaaactgat
    23401 caaccacgaa gatcttacat atgaagaatc actcgctgtt atgggcgaaa tgatggacgg
    23461 caccgcaact caggctcaga tgggtggatt tctgactgca ctgcgtatgc agggggaaac
    23521 cattgacgag atcactgctt ttgccacggt tatgcgtgaa aaaggtgtca aacttcaccc
    23581 ggaacgtgat gtcatggaaa ttgtcggaac cggcggagat gaagtcggaa ctttcaacat
    23641 ttccacaact tctgctttcg ttgttgccgc tgccggaatt ccggttgcaa agcacggaaa
    23701 ccgcagtatt tccagccgaa gcggagctgc cgatgtactt gaaaaccttg gtgcaaaaat
    23761 ggatctgaca gccgagcaga acgaaaccgt tttaaagaaa accgatatgt gctttatgtt
    23821 cgctccggtt tatcattctt ccatgcgtta cgctgctcct gtccgcaaag aacttggcgt
    23881 tcgtaccgta tttaatatct taggtcctct gtccaatccg gcggccgcca aaatgcagct
    23941 tctcggtgtt tacgacaaag atcttgcacc ggctctggct caggttcttt ccaatcttgg
    24001 tgttacccgc ggcgtggctg tctgcggcga tgacggactg gatgagatta ccctgaccgg
    24061 taaaacttac gtcaatgaaa tccgaaatgg tgaaatcatt tcttatacga ttaccccgga
    24121 acagttcggt ttaaagagct gcgagctttc cgaactgatc ggtggcacac caaaagaaaa
    24181 cgctgaaatt acccgccgta ttctgaccgg tgaggaaact ggaccaaagc gtgacgtcat
    24241 cctgatgaat gccggcatga gcctctatct tggcatcgac ggcatcaccc ttgcagaagg
    24301 tattgaaaaa gcaaaagaaa tgatcgacag cggaaaagct tacgcaaaac ttcaggaatt
    24361 tgtcaaagct acacaggagg cttaatttat gattttggaa cagattgcgg cagataccag
    24421 acttcgcctg atcgaggaaa agaagctggt ttccccggaa gaaatgaaag aaaaagctct
    24481 cgcccttgca gcagatgaga cttttccatt tgaaaaaatg ttgaaagaac ctggtattca
    24541 ttatatttgt gaagtcaaaa aagcttctcc atccaaaggt ctgattgcag aagattttcc
    24601 atatctgcag attgcaaaag aatacgaagc agccggtgca gatgccatct cctgcctgac
    24661 agagccgaaa tattttctcg gaaagaaaga atatttaaaa gcgatcacag atgaagtttc
    24721 catcccggtt ctgcgaaaag attttaccgt agacgaatat cagatttacg aagcgaaaac
    24781 actcggtgca ggcgttgttc ttctgatctg tgcactgctt gataccgaaa cgatccgctc
    24841 ttatattgaa atctgcgacc ggctcggttt gtccgcactg gtcgaagttc acgatgaaaa
    24901 cgaaatggat tctgcaatca aagccggtgc cagagtgatc ggtgtaaaca accgaaactt
    24961 aaaagatttc acggtagata tctccaacag cactcgcctg agaagtcttg ctccggaaaa
    25021 tgcacttttc gtggcagaaa gcgggatcaa aacagcggaa gatattcagg tattaaaaga
    25081 tgccggtgta aatggtgtgc tgatcggtga aacactgatg cgaagtcccg acaaagctgc
    25141 catgcttgca gccctcaatg gaggagcctt atgacaaaga tcaaaatctg cggtctgaaa
    25201 cgcacagaag atatttctta tgtcaatgaa ctgcttcccg attttgccgg atttgttttc
    25261 gcaaaaagca gccgacaggt cgatgcaaaa aaaacagcag aattaaaaaa acagctttcg
    25321 ccaaagatca aagcagtcgg tgttttcgtc aatgccgaac cggaatttat cgacaaatta
    25381 tatcaaaaac aaatcattga tcttgcacag ctgcacggcg atgaaaccgc agattacatt
    25441 catagattaa aaaatttatg cccttccctc ccgctgatca aagccgttcg cgtacaaagc
    25501 acgaagcaga tcctggagac agaaaagctt ccatgcgatt atctgctgct cgatacctgg
    25561 cagaaggatt cttacggcgg aagcggcaaa acatttgacc gttccctgat cccaccgctt
    25621 gcgaaaccat ggtttttagc cggcggactg aatacagaaa acattgcaga aagtattgct
    25681 cagtgtcatc cgtgggcggt cgatgtgagc agtgccgttg agacaaacag tgtaaaagac
    25741 aaagaaaaaa tcaaaacatt cattgatcat gtacgaagat aaaacattgt attatatata
    25801 gaaaggattc acttatgagc aaaggaagat ttggagaata cggcggacag tacatcccgg
    25861 aaacactgat gcccgctgtt caggaagtag aagaagctta caacaaatac agcaaagatc
    25921 cggaatttat cgcagagctg gatgacctgc tgaaaaatta tgcgggaaga ccttctctgc
    25981 tctatgaagc aaaaaaaatg accgaggatc tcggcggtgc aaaaatttat ctgaaacgtg
    26041 aagacttaaa ccacacagga gcacacaaga tcaacaacgt actcggtcag gttcttctag
    26101 caaaaaaaat gggcaaaacc cgtgtcatcg cagaaaccgg tgccggacag cacggtgttg
    26161 caactgctac agctgctgct ctgatgggac tggaatgtga gatcttcatg ggcaaagaag
    26221 ataccgaccg tcaggcactg aatgtatttc gtatggaact gctcggtgca aaagtgcaca
    26281 ctgtcacaag cggcacaatg actttaaaag atgccgtaaa tgaaacaatg cgtgaatgga
    26341 gcaaacgtat ggatgacacg ctttatgtac ttggttctgt catgggacct catccattcc
    26401 cgacaattgt ccgtgatttc cagaaaatta tcggagaaga aatcaaagaa cagctgatag
    26461 aaaaagaagg ccgtctgccg gatgccgtga ttgcctgcgt cggaggcgga agcaatgcca
    26521 tcggtacttt ttatgaattc atccctgata aagaagtcgc cctgatcggc tgcgaagcag
    26581 ccggacttgg cattgatacc ccgaaacatg ccgccacgat cgccaaagga agcaccggta
    26641 ttttccatgg catgaaatct ttgttctgcc aggacgaata cggtcagatc acaccggttt
    26701 attctatttc tgccggactt gactaccctg gaatcggccc ggaacatgcg atgcttgcaa
    26761 aagaaggccg tgcacagtac gtttccatca ccgatgaaga agccgttaat gcatttgaat
    26821 atctttcccg caccgaggga atcattccgg cgatcgagag ttctcacgcc gttgcctacg
    26881 caatgaaact ggcaccgact atggataaag ataagatcat cgtaattact atttcaggac
    26941 gcggcgacaa agacgtggct gccattgcac gatacagagg agtggatatc tatgagtaat
    27001 atatataagg cattcgaaaa taaaaaagca tttatccctt ttgtaacagg tggggatcca
    27061 gaccttgaaa ccacaaaaaa tctgctgatc gccatggaaa aagccggtgc cgacctgatc
    27121 gagatcggta tcccgttttc cgatccgatc gccgaaggcc cggtcatcca ggaagcaagc
    27181 cagagagcac tcgctgcagg atgcaccacc gataaattgt ttgatatggt aaaagaagcc
    27241 cgccagagag taaaaattcc gatggtgttt atgacctata tcaacccagt ctatacctac
    27301 ggaaaagaac gcttcatgaa acgctgcgta gaatgcggca ttgatggaat catcgttccg
    27361 gatcttcctt atgaagaaaa agaggagctt tccggtgtct gcgaatcata cggcatcgac
    27421 ctgatctccc tgatcgcacc gacctcccac gaacgcatcc gtatgattgc aaaagaagcg
    27481 aaaggatttg tctactgtgt atcttcactt ggtgtaaccg gcgttcgaag tgagattaaa
    27541 acggatgttg caaaaatggt agaaatggtg aaagaagtaa atgatatccc ggctgccatc
    27601 ggattcggta tctccacccc ggaacaggca aaaaaaatgg catccgtatc agacggagcc
    27661 atcgtaggat ctgcgattgt gaagattgta gcgaagtacg gaaaagaatg tattccggaa
    27721 gtcgaaaaat atgtgaaaga gatgaaagcg gcggtggaga tttgaatgtg caaatgaacg
    27781 aattccccga agggcacgta aaagtgcctt cttgggaatt gttcatttgc acatctcaaa
    27841 aagcccgctc aacggctctg caaactcccc gcttttttcg acatccagcg tacagatcac
    27901 tttgtcgcac ttcttgatcc attcttttgc tttctgtatt tctttttctt caatcctgcc
    27961 aaatggtttc acggagatca cttctgtcgc cagtgctttt gccgcgggat aatccagatc
    28021 gttttcccac agaattcccg tcacaaacgg cactcgctct ctctgtaatt ttcgaaagac
    28081 tgctgtaccg gttccgtttc ctgcgatcac aaagactttc ggctctcctt ctgttttcgg
    28141 aagttcgaga tctccggtgc gttcatcata agaacctgtt gtcatctgat aaagctgtgg
    28201 gatgtagccc tgcgtaaaaa tttcttccgg tgttccaaat cgatctactt tgtctcctct
    28261 gacgcaggct attttatcgg aaatacgtcc tgccaggtcc agttcatgca gggacatgat
    28321 tacggaaaga tttcgttccc tggacatatt ctggatgatg gataaaaatt ccagcttata
    28381 gcggatatcc agaaaagagg tcggctcatc cagaatgatg atatccggct gctggcagat
    28441 tgctctcgcc agcattaccc gctgtttctg gccgtcactc gtttttgaaa aatcacggtc
    28501 agcaagctcg tggatgtgca ccagttccat tgcctcatct accagtttcc agtcttcttt
    28561 tgagagtacg ccgaacttcc cggtatatgg ataacgtccg gtcgcaacca cttctttgca
    28621 ggtcatcatt tccggtctta cccgttctgt caacacaacc gaaagttttc tggaaagctc
    28681 ttttccgctg atttgttccg tctcttttcc atccagcaca acgactccgc caagcggttt
    28741 cagctgtttt atgatgcttc tcaggatcgt cgtttttccc gctccgttcg gtccgatcaa
    28801 cgtcaggatt tctccctttt tcagactgat cttgatattt tttatgagcg gaacgccgtg
    28861 atagccgacg gtcagacctt ccgtataaaa ataatattct tccatatgct attccgcctt
    28921 ttctaaatca aaaattcaaa aatatttctg acattcttgc tgcaagatat accttatgca
    28981 ttgcatgaac tgttccatga ttcctgattt cacacttttt ctttttttct gtgaatcata
    29041 atatagatta ccaccggtgc cccaaatacc gctgtgatcg tactgatgga aagttctgtc
    29101 ggagcaaaca tgattcttgc caagaggtca cagagcaggc agaacacaga tccgccaagg
    29161 aaacacgcgg gtatcatgat gatcggtttt gcggtgccga ataaattttt aaccagatgc
    29221 ggaacagcaa ttccgacaaa ggataccggt cctgcaaagg ctgtcacaca ggctgacaga
    29281 atactggata acagcacgag cagtatgcga aacagctgta cattcagtcc catattctgg
    29341 gcataagatt ctcccatctg ataggcactg atcggctttg acatcagaaa aacgcagatc
    29401 gaagtgatcc cgacaacgat cgccatgatc actacattat cccaggtaat cccggaaaaa
    29461 cttccttttg accagttatg cagattgaca atatcggcat cgtccgcaaa tgttaccaca
    29521 aaatcagtga tcgcagaaca gatatatccg atcataacgc cgctgacgat cagcatcgac
    29581 atctgatcgg tcactttcga catcaggatc acaaatccca tacagatcat ggaaccaaca
    29641 aaagctgcca tgatcatcac ccaggaactt aaatgcaggg catttccaag ggaagctacc
    29701 atgacaagtg ccacgaccag ttttgctccg gaggaaattc ccagtgtaaa cggtccggcg
    29761 atcggattat gaaaaaaagt ctgcaaaaga tatccggaca atgccagccc gccgcccagg
    29821 attgccgctg ccattgctct cggaaaacgg attcccatca caatatccgt aaaggtttca
    29881 tccgctccat ttccccacag cattttcggt atctccgaaa ccggtatatt tacacttccg
    29941 atgcatatat tcaaaaccaa aaataaaaat aacaaagctg caagaatgat aaatgcagca
    30001 ccatatctgc tttttttcat cgtatcactc caccttatac agataagtca ggtctgcatc
    30061 gtctccggtc agcatttttc gaatatcgga tgtgattgtc ccaagttcca ttgttgactg
    30121 atagagattc ttggtcgtgc agtatacatg tccttctttc accgctttga atttctcaag
    30181 cagcgggctt tttgccagaa gatcctgcat agaagacaat tctccttcta ttgtactatt
    30241 ataaataata tagtctgcat cttttgctcc ggcataaaat tcttccatct gaagggaaac
    30301 tgtagatgtc gcttcctcct cgtcacctag atcatcaaaa atatagcttc cgccggcaag
    30361 ttcaatcatt ttcggcagat aatccgcact ttttcgcaca tttgcttccc catttgtcgt
    30421 gatatagaaa aatgccaccg tttttccggt tgcatcctga tcactcacct gttcaaacgc
    30481 tttcgcttct gcatcaaacg cctgctctgc ctgatcttca tgcccggtca gcagcccgta
    30541 aagtttgacc cattcagtac gtccgagcgg atttgtctca tagctggaat gatcgaccag
    30601 aaccggaata ttaaattttt ccatctgctc tttcacttcc ggtgtatgca gaatcatggt
    30661 attctcgatg gcaagcccac agttttcatt caatatcatc tcatagtccg gggctgaata
    30721 tttcccggca taaataatat ccccatcttc catggctttt ttcgcctctt ctatgtacca
    30781 gccatctgct ttcaacgagc tgaatcgaac cgcatccagt gcatctgtcg ccacaaacat
    30841 atccatcgcc gccgaagccg ccagataaat attctggatc ggctgctgca gcaccgtgat
    30901 atccttatcc atatcttccg gcacagattc tccttccggt acgatcagat atttcccgtc
    30961 atctgcgatc gtgacaagtg cataatcatt ctgataataa tccaccgcaa acatctttgc
    31021 gtactccagt tccatgctgt ggtcgtatgt cagatccttg ctgatatcag tatttcgctt
    31081 ttcaatcgta tcctgttttt gcgatgcaca gcctgccagc atcaggcaga atacaacacc
    31141 gagtaccccc attattttct tcatttcttt tcctttcgtc tcatcctggt aacagccggt
    31201 atgaacacag ccactgcaac tgcaatccat aatgcgatat ttaccgatcc actcatacct
    31261 gatccggagc cggacgctgt cacggtgtct gatgcaaagg tcagcgtata ttcgatttca
    31321 tgcggtacac tcatcgcagt cgtatctgcc attaccgtca tcggtttatc aaattctgca
    31381 accggaacct caaaaacaga atttccttcc gtattgaccg gaagaaattt ttcaccgtca
    31441 accagcatat agtcataatg tgaactgctc catttaatct cagctgttac ttttccatct
    31501 tttaccgaaa tgtcagtcgg agaatcgatc gttgctcttc cgcttccgcc ttccatcgta
    31561 acttctgcgg tgtattcacc gtcttttaaa tttagttcct ctgctgtcat gatttctcct
    31621 tttcaagata aaaagctgct gcaaaatatg tagatacata tcgcagcagc ttttcgcaaa
    31681 atctgatatt ttttattctt ctgcttttac cggattggaa acgctgactt tatggtcata
    31741 ccattttcct tttgttccga tcagtgccag atcaaattcc tcatcgatag ccggaacagg
    31801 aacatcaaat gcattcactt cttctgccgg ttcaccgtca ttataatcta cggtttccac
    31861 aaccggctgc agcagttctg ctccatcttt ctgtgcatcc tctgcaagtc ccggatacag
    31921 atttacaatg tttgtagaac gcagcgtgat atgaatggtc atctttccgt cttttacggt
    31981 cagagttcct tttccatcat acatttcatt gacctggaac atactgctgt ctgtattaaa
    32041 atcagccgtg taagttccat cttccagtgt tgcagcggct tctgttgcct ctgtgtctgc
    32101 ggtcgtttct gctgcactct ccgcctcctg tgtttcttcg gtctgtacct gtgtttcaga
    32161 agctgcctct gtctgtgcag cactctgtgt ttctgtctgg cttgtgctgt ttccgcagcc
    32221 aactgccagt gcacagctaa gtgccattaa tcctgctaat gtaatgattc ttttcttcat
    32281 ttttgttcct gtccttctat tttgcttctt ttttcaagaa tacctctgac attcctgctg
    32341 cgaggtacac ctcatgtatt acttattact cgtttatagc atctgctgta tgagcaacgt
    32401 agagatcctg gattgctttg atctcaccca gacctgcgat ctgtgtatca atgctgtcaa
    32461 attttccgga tgcctcaaac tggctcttcc aagaatcgtc atcatcgcct gccatatcgt
    32521 tgtttgcatg atctccagca acaaccatca gcggacgaag gattactttt ttgtatcctg
    32581 ctgcagatac agctttgatc acttcttcgc atgctgtctc ttccggttcg ccttcaacgg
    32641 ttccgataaa tacattttca taaccaagct ctttcatctg agcctgcatc tggctgtaag
    32701 aaacttttgc tgtatgagaa gttccatgtc ccataaatac aaatgcaacg ccatcttctt
    32761 ttgctgcatc cagggaatca taatttgctg ttgctactgc ttcagaagtg attgcttctg
    32821 ctactgcttt tttatcatcg tttacagtat ctgcatcatc tccgacctca cccagcagcg
    32881 gttctgctac ttttacagat tcaaatttat ctttatatgc atctacggca tccatcagtt
    32941 cgtcatactc tgcaccatgc atcagatgtg taggctgaat aaccagattt ttaacgccat
    33001 tttttacggc acgttccaga gcctgatcca tgttgtcgat gtgctcatca tcacgagcct
    33061 ggatgtgatt gataatgatc tgtgctgtga atgctcttct tacagaccag tccggatttg
    33121 cagcctgcag tgcatcttca atacctttga tatcagcaac acggctgtca ttgaaagaag
    33181 taccaaagct tacaaccagg agttcgtttt caccgatctc atcaccattg cgcggatcat
    33241 ctttggatgc atcaccggta tctcttccga aataatccgg atcagcttct tcgccttcaa
    33301 ccaattcttt ctgagcatct gtcagagcat cccatgcttc ttttgcttct ttacactgct
    33361 cgtctgtgtt ctcatttctt tcctgaacat aaattgcatc gatcagagct gctactttat
    33421 ctgctgctgc ctgatcatct tctgcttccg tagctgctgc tgtttcagaa gctgcctcgg
    33481 ttgttgccgc ttcggtagct gctgctgtct cggttactgc agttgtctct gtcggagcag
    33541 ttgtctctgc tgttttgctt ccgcatcctg ccagcataga tgctgtaatt gttgttacac
    33601 ataaaatcgt taccagtttt ttcttcattt gcctttcctc ccaaaataat acttctattt
    33661 gaaacaaata agatttggga gaaaaagaaa atccaacgaa cacacgtcgt tggactgtat
    33721 actggataac aacagccaaa ccagaatttc ttcggcttca ctgtttctat ttcaattcgg
    33781 tacaatcaat cctcgtgacc tggaatatct attccccgca ccacagtctg gcaggtctcc
    33841 tggcttacag ttttaacgca cattgctgcc ttcccgatat ctcagtgaca taataagcaa
    33901 tacactcctg tttacagtga cgagatcgca cgggtctttc acctgtttcc ctattctccg
    33961 ccttgcggcg gcaccagctg cttcttattc tatttcaaat attcgttcct aacagtccga
    34021 taattaccct ataacctatc atttatatct gactgttatt tacatttcca aattatagca
    34081 taaattttaa aataatcaac tgttttatgt tattttaact caaggcacaa ccataaaaaa
    34141 caattatcta tatcaattcc cgttactacc ataatatcga taaatctgat tcatacctca
    34201 ctttttcttc cttttttact tagctattta ttaataattc ttctatttta caggtatgtt
    34261 tttttgttat tttatcatat gaaatcccca ctgccagaat attttgcggg tattgtctgt
    34321 gttctcccag ttttcctttg aaacgtaata tataatcttt ttctttaatc tgtttgatag
    34381 cttcttctgg tgtctgatcc acttttaatt ccaatataat gcccttatct tcatatctga
    34441 caggataaaa aataaagtcc acaaatcctt ttccagcttt atcttctctt tctatcctat
    34501 aaaaatctct tgcagaaaga taaaccagat ttacaatcgc cgataattcc acctcattat
    34561 tataagataa gattggcgtt tccgtattat gtgcatattg caaaatttca gccatagtat
    34621 ctgtatcttt atttaaagtc gcctgtaaca ttttattaga aatatttgca agtctgtata
    34681 tatatcccag ggatttttcc tttttcatca tagatgcata gcttccctgt aactctttat
    34741 ttggaataaa cacgcagcca ttatcatacg tcaataaccc ataaacaacc atcgccgaat
    34801 atatttcttc ctttgtatcg agctgctgtg ccgttgctgc atattcctca atacccgatg
    34861 gtattttttc tcctgcaaac atgagaacca gatcttcctg tacgtcatct atattgtttt
    34921 taacatagct gaatacggaa tcatattttc ctgagctgac ccagtaattt gacagctgat
    34981 tatcggacaa tgcacataca accgaacgcg gattatacaa tctctgaccg ga
//
LOCUS       DAWDWH010000003        29850 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_906_length_29850_cov_15.862091, whole genome shotgun sequence.
ACCESSION   DAWDWH010000003 DAWDWH010000000
VERSION     DAWDWH010000003.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 29850)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 29850)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29850
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_906_length_29850_cov_15.862091"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         8978 a         5960 c         7076 g         7836 t
ORIGIN      
        1 ggaaagggag atgcaaatga aaagtgtaaa gaaattcagg gcttttttag gtggttttat
       61 tctttttctg gcactgatgt tcacagtggg ttctgtggat gtcaaagcgg cggcaccgaa
      121 gctgaataaa aagaagatta ccatctatgt agggcacacc ggaaagctga aagtaaacaa
      181 tgccaggaag aaagtgaaat ggacttcttc gaaaaagcag atcgcagctg tttcttctaa
      241 gggagttgta aaagggaaga aagcgggaag tgcggtgatc actgcggcag caggaaacaa
      301 aaagtggaca tgcaaagtta ctgttcgaaa taacgtatcg gtaagcaaag gtgcaatcaa
      361 gattttgccg ggtaagtcaa agaaggtcaa tattaaaatt cagaaagatg taaataacat
      421 acgatttaaa attaaagatc caaaggttgt ttcctgtaaa tggggcaaat ggaaaggaat
      481 gtcgattcct ctgacaatta cagcgaaaca gtccggttcc acttatataa cgcttacaaa
      541 tacttacagc aaagaaaaag taaaaattaa tgttactgtc cctgctgaat gggatgatgt
      601 aaaagtagta attccgaaaa ctattggtga acagggttat gagtcaaacc gtatgaagat
      661 cactaactac agtttttata aaccatatga ctggcaggat ggctactata tgaatgtaga
      721 tttcaagatg gtacaatatg gcaagccggg aaaaacaaac tggggcgaat atgttttctt
      781 ttatgataag aatggaaata tcctgaataa agcacagctt tttgcaagca gtctggcggt
      841 aggaaaatct tttaatgatg aactgtcagt tcctaaagga acagccaaaa tcgtatttat
      901 ggagtatcca gatacgacag gagataataa taacagcggc aatgataatg gcggaacaca
      961 ggatggcgat aattcctcca caaaagatcc tttaaaatgg gacgcttctg atcttaagaa
     1021 aatgaacagt tatgcggata aggcggctga atgggcaaaa aaagcatttg attatgcatc
     1081 caaagccaag aaacagtcag gcatgagcca gaattatacg gcaatggcaa taacagccct
     1141 taagatggca aaaagtcata tgcagaatgc ctatgattat gcgattgtga aggaggatgc
     1201 aaatctgata gacgcagatg gcaatccggc tggtactttg caggagcgca cgcaggaact
     1261 tttgagtgta tttgaaggat tggacgatct gacaaataca gatacagata cggtttataa
     1321 tatggcgacg gatgccatga caaaaacggc tacatacaaa gttttgacag caagattgct
     1381 tctgtaaaac cctggtggaa atagcacaat ggatttttta aaacgttcac aaaaaatctg
     1441 acaagtccca aaagcagaaa caaaaatgcc ctgaacttcc ggagatgatc ctacagatat
     1501 ctcctttggt tcagggcatt ttttatttta tatttccata ccgatcaaat gcgttcgact
     1561 tgaatgagca gcaagtgatt tgacctatct ggaattattt tgcaaattct tccagcaatt
     1621 cagcgatacg atcctggcaa ccgccgcagt gggttccggc ttcggttgct tcaccgattg
     1681 catcaacggt agtatgacct tcgtcatacg cttttttgat atctccggct gtcacatcag
     1741 cacagaaaca gatggtttca tttaaatcca tactggcacc tccgtggaaa tgaatttatt
     1801 ctggtcggag aaaactccaa cttctgcgtg cgttcgactt gaatattatt ctccgaagta
     1861 tctcttaagc agacctgcga atgcttttcc atgtcttgct tcgtctcttg ccatttcatg
     1921 aacggtgtca tggattgcat ccaggttcag agcttttgca cgttttgcca gatctgtttt
     1981 acctgcggta gcaccgtttt ctgcttctac acgcatttcc aggtttttct ttgtgctgtc
     2041 tgttacaact tcaccaagca gttctgcaaa cttagcagcg tgttcagctt cttcataagc
     2101 tgctttttcc cagtaaagac cgatttccgg ataaccttct ctgtgagcaa ctcttgccat
     2161 agccaggtac atacctactt cagaacattc accctcgaag tttgctctca ggtcagcttt
     2221 gatgtcttcc ggaacatcag aagcgatacc tacttcgtgc tctgcagccc atgccattgt
     2281 tgtatccatc tctttgaatt tagaagccgg tgctttacat actggacaga cttccggagc
     2341 tgcttcgcct tcataaacat aaccacatac tgtacataca aattttgcca tttttttgat
     2401 ctccttttca ctttgatttt taataagcta atcctgggat atccaaaact tttacgctct
     2461 gaacattcac aggttgaatc cttgctatta ataatagtaa ttgttactga tattaagata
     2521 gcacatcttt tgctttccgt caagactttt ttaatagttt ttttcattaa gttttgcggg
     2581 gtttttactg tagtgtgttt aacaatagaa ttttcctgtt ttagcagata tctatattat
     2641 gctaaacatt ttttgcagat tccgtaaaaa gtagtcgtgt gacttctgat tttgccatca
     2701 aatttttttt gtgctgcgat ggtgatctcc tcgttatcat ccaggaagat atcttctaca
     2761 caatggcatc tttcacacac aaagtgatcg tgtggtgtga tcctgccatc gaaacgatca
     2821 gcactatctc gcatatttaa cttgaggatc tgtccggtct cagagagcag ggacagattg
     2881 cggtaaaccg taccgagact gatatttgga aagctctccc gcaaattcat gtaaaccata
     2941 tcagcagtcg gatgttcttc cgagtgagca agaaaatccc ggatggcttc tcgctgttta
     3001 ctgtacttta aaactgccat aatgttctcc tttagataaa tgatgccaag cttaaaaatt
     3061 tcaagttaat cctgatatca taatattagt aatagttatt attataataa tctaaaaaaa
     3121 accgtttgtc aatgctttta ttttacaaaa agattgacta tactaatatc ggatagcatt
     3181 tcaggaatat acggtaagca acaggaaaat aatggatatc aggaggaaat acaatggaaa
     3241 aaacatatga tctgatcatc attggttcgg gccctgcagg tttaagtgct gcgatctatg
     3301 cccagagagc gggactggat actctggttc tggaaaagga atatatcagt ggaggacagg
     3361 tagtgaacac ggcagaggtg gataattatc cgggactgcc gggaatgaat ggatatgagc
     3421 ttggcatgaa attccgttct catgcggatg cactggaggc gaaatttcag attgcagaag
     3481 taaaagacat tataatagaa gaaacacaga aaacggtgcg tacaaacgaa ggtgattttt
     3541 atgcaaaaaa tattatcatc agtacggggg caagccacag aaaactgggc gttccgggtg
     3601 aaagagagct ggctggcatg ggtgtttctt attgtgcgac ctgtgatggt gcatttttca
     3661 agggaagaac ggttgccgta gtaggaggcg gagatgtggc agtcggagat gccctgtttc
     3721 ttgcaagagg atgcaagaaa gtttatctga tccacagaag agatgagctg cgtgcggcaa
     3781 agagtctgca aaagaaatta atggctctgc cgaatgtgga gattatctgg gataccgttg
     3841 tcaatgaaat ctgcggtgaa gatcaggtag aggcactgtt tattgaaaat aagaaaacag
     3901 gtgcgaagaa acatcttcct gtggatggtg tgtttgtagc tgtcggtatg aaaccaaata
     3961 cgaacatggt tccggatgaa attaaaaaag atgaggcagg ttatatcgtg gcagatgaaa
     4021 attgtgtcac aaatgttacg ggaatctttg cagccggtga tgtgcgtaca aaaatgttac
     4081 gtcagattat tacagccgca gcagatggtg caaatgcagt agagtccgtt cagcacggta
     4141 tttaatgcta atatccggaa caaagcggaa tatacgtgaa ttgcagaagt ataaaataat
     4201 ttgcagagtc tcttttaaag tcaaatacgg cttcatatct gtgaaaaaat atttgaaaaa
     4261 tgtatgaaat ccttataaaa gatattaatt actgttgaca aaacacctcc ggtttgttat
     4321 gattagcgtg taaaaaatgt aacagttttg taataaatga aaattacaca gtgttttcaa
     4381 tacatggagg tagatataag taatgaaaag aggagtagta aaagtcactg catgttgtct
     4441 tgcaggaagc gttattttcg caagcaccgg tcttaccgct tttgcagcag gggagatgcc
     4501 attagcagga atcggagctg agattattgc aagtcaggaa aagaaggaaa attccgaaac
     4561 aaaaaaagtg attgcaaccg gttatgacag tctggtaatt gctcaggttg atgaatatgt
     4621 aaatattcgt gatgaagcaa gcacagagac aggtcagatc gttggaaagt tatataacaa
     4681 ttcagcagca gagatcattg gtcaaacagg tgactggtac ttgatcaaat ccggagatgt
     4741 tacaggatat gtaaacaaag attattttgt aacaggtgca caggcagaag agctggcagc
     4801 agaagtcgga gatgatgtag ctacggtaaa tacagagact ctgatggttc gtaagaaagc
     4861 aagcaccgat tccgatatta tcgcactggt tggagactca cagcagcttc aggttattga
     4921 tcaggaagac ggctgggtaa aagtagcagt tgataatgac gtagttggat atgtttccag
     4981 cgactatgta gactgtgaaa caaaatttgt agaggcagaa tccatcgaaa cgagtacagc
     5041 aagagaagag gcagtacaga gtgctctgga tcgtgcagat cagatgaaag aagcagcaat
     5101 caatgcgatg aacaatgcgg atgcaaacga agcagcatat gcagcacagg aagcaatcgt
     5161 agcagcggca gaagcaaaac agcttgcatc tgagcaggaa ttagattata acgtacagga
     5221 aatcgcttct accgcagtaa gttctgcaga tgaagcacag tatgcagcat atatggctga
     5281 acagtatcag gcagcagcag aagcggaagc agcacgccag caggcagaag cagaagcagc
     5341 gtatgcagca cagcagcagg cacaggcatc tcagcagcag cagaatcaga attggacagg
     5401 tgatcagaca cagcaggaaa cacaggcacc gtccacaaca acggatacaa cgacaacacc
     5461 ggattacaca acaggtgata cgacaacaac accggactac acaacaggtg atgcgacaac
     5521 aacaccagat tacagcacag gtgatgttac cgttactccg gattacggca caggcgatac
     5581 aacgacaaca ccggattata caacaggtga taccaccgga agtacaacga cagacagctc
     5641 tgcaccatcg ggttctgact ataccggaga tgttccacag tcagatgcaa gcagttctga
     5701 ttctctcaga cagagcgttg taaactatgc acttcagttt gttggtaatc catatgtata
     5761 tggtggtaca agcctgacaa acggaacaga ctgctccgga tttacacagt ctgtcctggc
     5821 aaacttcgga atcagcattt cacgtacagc tgcatctcag tcaggcggcg gtactgcagt
     5881 agatatgagc aatttacagc cgggcgatct gttattctat gataacggaa gcggtatcgg
     5941 acacgtatcc atgtacatcg gcaatggtca ggttgttcac gcaagtaacg aacagaccgg
     6001 tatcatcgta tcaaacgtag attatagaac cgcttgtgca gcaagaagtt acttttaaga
     6061 ataagattaa ttgaacagag aactaaaacg ggagtgttcg caagaacgct cccttcttac
     6121 gttataagga aaaatactgc gtttctgcgg agtgtttatc ttaattgaag gttgaaggat
     6181 gataagatag atttgctgca cattgtttgc ggaagcggaa gtcttctgga attaagatga
     6241 aataattaga taattttaaa tatttacacg ctgattaaga caattctgat taaatagaaa
     6301 aacaataaaa aaagccgttt ggcaaaatgc acaaaagatt tgagatataa acagagtgtg
     6361 gataaattca tgtggattca cgcacgagtt atccacagtg aaaaatgtcg aatttgctga
     6421 aaaatagagt tatacactga gttatcaaca ttatccacat tttttgtgca caaaggcagt
     6481 gtttataaat tccaaaaaac aaacgcacgt tttgtgaaga aatgataagt gatgttttca
     6541 aacgtcaaaa aagtgaaaaa tcgcatattt gtatacttat aaaaaatgag tgaattatca
     6601 gaaaattata atttgcccat ggcataatcc tcttaagtac aagaatgtcg ctgatatatg
     6661 attgaaaaca ggatattgtt acgttataat tttgctatat gggggggatt ttataagaaa
     6721 tagaatgaaa atattatgag acgaaaaaaa ctttttgcgg cgatgcttgc agcagccgta
     6781 gcatttgcgg catgtggttc tttgcagtat attacgttcc cgaaaacagt gacaacagtt
     6841 ggaacgaagc ttgttacaga tttcacttct ctgctcgcag tacgttacac cggaacagag
     6901 gatgaatgga atgcatctga ctttaagacg caggaattca gcgagttacc tgcgtgaagt
     6961 gcggtctgaa gaaagaggag tccattccaa aaaaagaaca taatttttca gaatggtgtg
     7021 ttatcataca gcctacaacg gaaagggaac agcggtgatc actgtaaaat ccggaaaaat
     7081 aacagcaaaa tgcaaggtaa aagtaaaatg atgtaatttg atttgcagat atagaatccc
     7141 cttttggaaa atcctgacag cagttgtgtt gtacctgatg ttgccggaat atctcagggg
     7201 gattttttac agcaaccgga acaggaaaaa agacggaaga cctgagaaag gagatagaac
     7261 caaagatatt acagggagtt tctaagatgt agtatactga tgattgtcaa aagtaataaa
     7321 tgaaaataag aagtaaaaga atcaggaggt tcagatgaag aaaaaatatt atggaatatt
     7381 gctggcagca gtgattgccg ccggcacaat aggtgtaaca gcacaggcag cagaactggc
     7441 agatacggaa aaactgacgg aaactgcaaa tgtaacagac gatgtaaata cagcaaacag
     7501 cacagagtga tgaactggaa gtgccactgg tactgaacca gacatacacc agaagtttct
     7561 cacaggtcgg acagaaatat tatttcacgt ttacaccgga agaaacagga tattattatg
     7621 ttgatcttaa tttcccggaa aatatgaaca ttcacgtact ggatgattac tcttccgatg
     7681 cactgtattt tgcagaaaag ctgaacaata cagaatctta tacattctgt ctggaattgc
     7741 aggattctgt agatggacag atgcagggaa cgggtacgat caccttccgc aaagctccag
     7801 atattaaaaa cgttaccctg cagaaagcaa atgccagaga tgttttctac aatgcagatt
     7861 ttgaaaccat ttccccggat ggggcgaaac tgacagtcga atatgaaaat ggcacaaaga
     7921 cttattcgtt tgattgggat aatggtactg ctttttctct tacagatgat tataaatatg
     7981 tatttaatgt tatgtttctg gatgcaaacg ggaatgatat atcaaatgtt gtaaacaatc
     8041 cgggtatggc tggtgcaaag ggaaattacc aggtcagagt gtctctgaaa acgttatcca
     8101 tgaacagcga taatggagag aaggagaaat tgacaggaac tggttattcg atcacgatac
     8161 gtgacctgtc actgaacagc tccaacaaac tggatatgaa aaaatcctat ccttctttaa
     8221 agtacacgga cggagaaact gcgtattatt ggtttacacc ggataaaagc ggtgcttact
     8281 atgtgagcgt caccggatat ggtgcagact actggcagaa atcctgctgg ttccgtgtat
     8341 acgaaaaaac agtcagcgga tatcagtcga aaggaatggc gatcggtaca ctgaactgca
     8401 agctggaaaa aggaaagcag tatattttaa ctgctgatca ctatggaaaa tccagtgttt
     8461 ttggtgcatg ttttgcaaaa gcagatacgt atgttccact ggaagttgca cagacaccat
     8521 acgtggaatt gaatgtgaag tcggatgtga cggttccatt gaaagtgaaa agctcttaca
     8581 aaataaaaac cataagaatg ccttcgactg acaggattgt ttcttataag agcagtgata
     8641 caaaaacagc aaccgtttct aagaatggta cgatcaaagg aaagaagatc ggaacagcaa
     8701 aaattacggt aacacttgcc agcggttaca gtacttcatt caacgtaaaa gtacagaaaa
     8761 agaaagttgc cacaaagaaa attacagtgg acaaaaagaa gattacttta aagaaaaaga
     8821 agagctataa aatcaaagca gagcttcagc cgatcaccgc ttccgataaa gtaacattta
     8881 ccagcagcaa taaaaaagta gctactgtaa atgcaaaagg agtggtgaaa gccaagaaag
     8941 ccggaacagc gaagattacg gtcaaagccg gaaagaagaa agcgatcgta acagtaaaag
     9001 taaaataaaa attgaccgtt ggtttcccgg caggtgtaaa gaactgaaat gccgcacatt
     9061 ttaaaatagt gtttgcaaaa gaatagtatt gcatgtgcaa atgaacgaat tccccgaagg
     9121 gcacgtaaaa gtgccttctt gggaattgtt cattttgcac attgctgtac gtttttgaac
     9181 ggccatagtt tttaaaaaca cttcatatca tattttacac gctggcagga aacagccttt
     9241 tgccacgctg gtgcacgcag aaagttttta gaatcaaaac gttccgcatc aactttcttg
     9301 tgtacaaaac aaaaaagaca gaggcacagt gacggaaatt tactaccgtt acagtgtctc
     9361 tgttttttcg tgttttgtaa tttggcctga aggactgttc cgcttaagcg acccctggca
     9421 agccaggaac ttttggcaat gtgcaaaatg aacaattcct aagaaggtac tcgaacatgg
     9481 ccggtactta gcgaccgtat tttggtcggt tatgtaccgc tgccaatgtg aggtagcgag
     9541 agcgtgcctt cgaggaattc gttcatttgc acattctaaa acctctctgc atttgcttac
     9601 cagtagcgta caatcgcacg gattgtagca aagttaaatg gtgaggtctt aataccgccc
     9661 accgggtaag ctgcctctgt agaggcgtgt acaatcagcc cgtttccgat gtaaagccca
     9721 acgtgtgaga ttggctggta ataaaatacc agatctccgg gctggatatt gttgatggaa
     9781 atgatttttg cggtagcata tttggttgtt gtgccatcgt agccgattgc ctgctggtcg
     9841 gcggtacgag ggatcttgat gccaaaatgg ctcatgacgg actgcgtaaa accggagcag
     9901 tctgcaccgt ttgtcaggct ggttccgccg taaacatatg gatttccgat aaactgagtc
     9961 gcataagctg caattttgga accgaatgtc agatcggaat cggaagcatt ctggtcagtc
    10021 atatatccat ttgcatcgaa agagtagaat acgttgtcaa tgatcatgat ctggctcttg
    10081 accatttcac cggtcgtcgg aagaaaatag aacgttgcga cgctgttatt ataccatcct
    10141 gtaaccatga ctccgttacg gtcaaataaa taggttttgc cgttcagggt aacaataccg
    10201 gttgctttgc ttccgtcgga agtaaaatag taagtcttat catcaatctg cagtttctgg
    10261 ctggtaactc ttgcaccagt ttctcctaca taataattgc ctacccactg gttggtgagg
    10321 acaatgccgt tctcatctgt ataataatat ctgccgcttg cagaaacgac ccactggttt
    10381 tttgctttct ggtaattgga atccaggaag taggttttgc ctttctgtgt ccagattccg
    10441 gtagaacgtg tctttttggt acggtttccc tttttgttta tatagtaagc acctacccag
    10501 cagctttttg ccatctgacc gtttgatttc aggtagcgtt tgccgatcca ggtatcggtg
    10561 accattttac cgtttgcatc aaagtaataa tatttactgc caaccagtac ccagccggtc
    10621 atcatctttc cggttgtcgg atggaaatag taagtgtcgg caccaatctg agcgaaacca
    10681 taagtacgtt caccggcggc atttacataa tactttttgc tgatgatcct gttggtgtac
    10741 atacggcctt ttttaccgaa gtaataatat ttgcctttga tctttttcca ctttctcggt
    10801 gcagaaccgt ctgttttaaa gaagtatgta tagttgccga tcttgttcca gcctttcagc
    10861 atcaggccgg tttcagcatc aaagtaataa tatttgccgt tgtatttgag ccagccactg
    10921 taacgttctc cagtttcggt attaagataa taagttttgt cacggatacg cagccagcct
    10981 tttgcaagtt tacctttgta gttgtagtaa taatacttac catcattctc tacccaggta
    11041 tttttgtttt ccgtcacgcc gggaatcagg atgtcagaac cgtctgcaga agaaggatcc
    11101 cggacaatgt ttccgtttgc atcaatcgtg taactttgat ccccggaggt aatcacagtg
    11161 cttgcagcca tggagccgtc agcagaaaga taatatccat ctacccagcg attggcgagc
    11221 agcctgcctt ttatatcaaa atagtaccat tttttatcga tcaatttcca gccggttacg
    11281 gcgtatttgt ttttttcaaa atagtaagta tacttaccga tctgcttcca gccaacagcg
    11341 tagcccggtg cagccttgat ggtataccag cggccgtttg catctgccct ccactttgct
    11401 tccgctttca tagaaggact gactgtgcca agtgctgctg ctaaaagcat ggtaacgata
    11461 atctgctttt tcataaattg ctccttttaa aaatcatgaa gatcaagatt ttgaaatcat
    11521 gttaaatata ttacaaaatt attacataag aattttacag gatttagctt agaaagtcaa
    11581 gagaaaacac atattctgac aggtaaatgt cagcagatct gaccgggatc acattccggg
    11641 tcttaagtgt gttctgcctg aataaagatt ttacgtctga aatatgagag aactgtggga
    11701 gaaaaaagac agaacccagg ggacaaaaaa ttgtccgcca ggttcgggaa atccacttgt
    11761 taagccttct tgctctgcat tcttttgatg accttcagtc gggtgaattc ttcacgctct
    11821 ttttcttcca gtgcattctg aatgtctttt gtaagaacgg tataacgtgg gatggtaata
    11881 tttttcagag cgttggcacg cttctgggtt tttttgatat tgtttgccag ccggtaagct
    11941 gaattttcga tcatggaaag cttgatggaa agctgcttta ctttttcaaa tgaggctttt
    12001 gcctggtcca gtgcaatgtt tgtactgtag taagcgtaag tcgggaaagc aggtctggta
    12061 tcagcttcta ccagggggat ctccgtaccc atgatacttc ttgtgcggat gcggatcgaa
    12121 tcctcaaccg gtacggagta ggagatttct tccacaaggc tgatacccag tttgatgttt
    12181 gccatctgca gtgcagcata tgccatgcgg aaggtagaat caatttcttt ctgaatggat
    12241 tcggcctgct ggatgagtcc cataagttcc ctgatcagaa tatttctttt tttgtccatc
    12301 agcacatagc cctgtttggc aagtgcgagg gagttttttg ccagaattaa attgcccttc
    12361 gtagggaagg tattcggatc cataggctca cctctattcg ttataatatt tatccaggat
    12421 cttcgtatcg atacggtcaa gttcttctct tggcagcata cgcagaagtt tccagccaag
    12481 atcaagagtc tcaacgatag aacggttctc gtcctcgccc tgtccgacaa aggtatgctc
    12541 gaatgcctgt ccgaatttca gataggtttt atccagagga gaaagttcgt cctcaccgat
    12601 aacggatgcc agggctcttg catctccaac ttttgcgtag caggagaata actggtttgc
    12661 aacatcctga tgatcttctc ttgtgtaacc tgcaccgata ccgtccttca tcagacggga
    12721 cagagaaggc agtacgttga tcggcgggta aattgcctgt ccgtgcagct gacgatccag
    12781 tacgatctga ccttcggtga tataaccggt caggtcaggg atcgggtgtg taatatcatc
    12841 attcggcata gtcaggatcg ggatctgtgt tacagaacct tcgacgcctt ctacgatacc
    12901 ggcacgttcg tagattgtag ccagttcact gtagaggtat ccaggataac ctttacggga
    12961 aggaatctca ccttttgagg aagaaacctc acgcatggcc tctgcaaagg atgtcatatc
    13021 ggttaagata accaggatgt gcatgccttt ttcaaatgcc agatactctg ctgcggtgag
    13081 ggcaaccttc ggtgtgatca gacgctctac aaccgggtcg tttgccaggt tcaggtacat
    13141 aactacgtgg tcagaaacac cgctttcttc gaaagtcttg cggaagaatt ctgctacgtc
    13201 atatttaaca cccatagctg cgaaaacgat cgcaaatttc tcatcggaat tatcacccag
    13261 agaagcctgt tttacgatct gagctgccag acggtcatgt ggcagaccgt ttccggagaa
    13321 gatcggaagc ttctgtccgc gaatcagagt ggtcagacca tcgatcgcgg aaataccggt
    13381 acgaatgtag ttacgcggat attcacgggt aaccgggttc agcggcagac cgtttacatt
    13441 gatgtgtgct tccggataga tatcacccag accatcgatc ggctgtccga taccattgaa
    13501 ggtacggccg aggatgtcca gtgatacggc aagctccatc ggatgtccgg tcagctttgt
    13561 gtgggtattt ttaagggaca taccttcatt tccctcaaat acctggataa cggctttgtc
    13621 ttcgtagagc tctacgatac gaccgagttt cttttcgttg ttatccacaa cgatctctac
    13681 gatttcttca aaggaggcat tctgcacgcc ttccagaacg accagagggc cgttaatctc
    13741 gttcaggcct aaatattcta ttgacatttc ttttcctcct aagcgttctt ttcaagtact
    13801 tcgtcataga aacgatcgac ttctttgcgg tagtcatcga acatatccag acggtcattt
    13861 ggaacatcgt atttcacgga aatgatgcgg tcaaaaatgt tgctttcttt caaaatggac
    13921 atcggcatac ccatggtaac cagggctttt gcctttgtat tcagatacag gatcaggtcc
    13981 atcatcagga actgtttgcg gatcggaaca caggtatcgt ctttgtggaa tgcattctgc
    14041 tgaaggaatc ccagacggat gactctggcg atctccagtg tcagtttctg gtcatccgga
    14101 agtacatcac taccaatcag ttttacgatc tccatcaggt tgctttcctg gttcaggatc
    14161 gccatcagct gatttcgttc gtctacgaaa ttcttgtcgg cattgtcacg gtaccatggt
    14221 gccaggtcat ttacatattc actgtaactg gtcagccagt ggatagccgg gaaatgtctt
    14281 gcatatgcca gggatttatc cagtccccag aagcagcgta cgaaacgttt ggtattctgt
    14341 gtgaccggtt cggagaagtc accgccctga ggggaaaccg caccgatgat ggaaacactt
    14401 ccttcggagc cgtttaaggt ctgcatcata ccggcacgct cgtagaaagc ggaaaggcgg
    14461 gatgccaggt aagcagggaa accttcctct gcaggcattt cttccagacg tccggacagc
    14521 tctcgaagtg cctcagccca acgggaagtg gagtctgcca tgatcgcaac gtcgtaaccc
    14581 atatcgcggt aatactcagc cagtgtgata ccggtataga tagaagcctc acgggcagct
    14641 accggcatgt tggaagtatt ggcgatcaga gtagtacgat ccatcagagg tttgccgctt
    14701 cttgggtcaa ccagttcaga gaactcttcc agaacctgtg tcatctcgtt accacgctca
    14761 ccgcagccga tgtagatgat gatgtcggcg ttggaccatt tggcgatctg atgctgtgtc
    14821 atggtttttc cggtaccgaa accgccaggg atagcagcag taccaccttt ggcgatcggg
    14881 aacagagtat cgaggatacg ctgtccggtg atcagcggtt tgcttgctgc gaatcttgca
    14941 gcagtcggac gcggaacacg gatcggccat ttctgagcca gtgtcagttt ttcttctttt
    15001 ccgtttgcaa gctgaacggt tacgatcggt tcgttgatcg tgtattctcc atccattgca
    15061 acttccagca cagtaccgga aatgttcgga ggaaccatgg atttgtggac gattgcctgt
    15121 gtttcctgaa cttctgcgat gatcgtgccg ccagaaacaa attcacccgg ttttacggtg
    15181 atatgtgtct gccatttttt ctccgtatcc aaagaatcaa cgctgacacc acggctgatg
    15241 tatttaccgg aggattttgc gatctcactt aacggacgct cgataccgtc aaaaatattg
    15301 ttgaggatac cagggccaag tgttacggaa atagcatcgc cggtagcttc taccgggtca
    15361 ccaggacgaa gtcctgtggt ttcctcgaag acctgaatgg tcgtgatatc gtttgtcaga
    15421 cggataactt cgcctaccag acgttctttt ccgacgtaga ccatttcagc catctgaaat
    15481 cctgtgttgc ctttcaggta aataacagga ccgttaatgc cgtaaatagt acctgtttta
    15541 tgcatttgta gcacctccgt taaatgagaa gtctgctttt aacttttcca gtttggaagc
    15601 gaaagaattg tcgatcagga tgttgcgatc ctgaagaacg gctcttgtgc cgcccataaa
    15661 ggaatattca gaaacggtaa caggagctcc ggttgcgacc tggagactgg aacgtttgtc
    15721 ggagtctgca gggtcaatgt aaaggatcag ttttccggaa ccggcctctt tctgaatgtt
    15781 tttgatctgt ttgatcagca gctgctgata tgctgaagta gtcatgtaat cttcaagcag
    15841 ctgttttact tcgacaaaaa gcttttcttt cagttcattc tgtttctgtg tgatcttacg
    15901 cttgatctga agctgctctt tggaaagagc catattgttg tttctcttca gattgtcgct
    15961 ttctgttttt aactgtaaat cagactggcg aagagtggtt gctttgtgtt ctttttctac
    16021 cttgtccaga tttactttat attcttcaat catggcagaa gcctgttttc tggcctcttc
    16081 cagagaagat tcttcaaaat atttaagctt ttcttccgta gtcaaaatat ctcaccttct
    16141 ttataatttt attccgattg cttcgcttac ataggaagtg ataaagtccg gtttgcggcc
    16201 ggtaccgtgg cggtcaggga tttcgataag aagtggaaga cggcggttta agcggacatc
    16261 atcaataata tccggaaact cccgaccgaa tttttcggtc aggagaataa taccgttttc
    16321 tttattggca attgcatctt ccagtgcttt acgaagttcc gcacgttcgt gcacgataca
    16381 gccttctacg cctgccagac gcatgccggt cagagtatcg acgttatcac tgattaagaa
    16441 cattttcata aattaattgc ctcctgatat gcgaacagga aattaaagtt taccaaggat
    16501 catgaaggag atgatcagac cgtacagagc gataccttcg gccatggcta cgaagatcat
    16561 ggatttacca aagatgcttc cgtcttcact gatagcaccc agagcagaac ttgctgcgtt
    16621 tgcaacggcg ataccaccac cgacgcagga aagaccggtt accagtgcag ctgccagata
    16681 acccatacca gctgccagac cgcttgcaga tgcagaagca gtatcagctg ccagtgctac
    16741 agaaccgtcg cctgtgaaga tcatgataac ggcaagagcc atggttccaa agaagaggaa
    16801 gcagttgatg ccaagtgtgg ttttgaagcg tccacggttc ttttcaccga tcagaaatgc
    16861 tgcggttggt acgacgatgc tcagaagcag tgctgcgata aaaatgaatt ttgtaatagt
    16921 tgacatagta atttcctcct taatcgtgtg tgattatgaa ctcacaaata taaaatattt
    16981 tatttccgtt attttgcttt taaatgttaa tcaacggatt ttttaacaaa tggtgtgaat
    17041 ggatgtcctt cgcctttata gaaacggctg aacatttcgt agtattccag acgaagtacc
    17101 tggataccaa cgatcagacc ctccattgca catacaaaaa cgttaccaag tacgataacg
    17161 atccagttga tgtttccgga ttcggctccg gcaagcatca ggactacttc catcatagct
    17221 gcgtggctga cggcgaaagc accgatacgc acgaaagaca gcgtgttgga gaagtagctc
    17281 agtaatacat caaacagttc gaaaaatgca gttacaagga acatgccttt ttcttttggc
    17341 atagcatctg cttttttctc gaccagttta ccgagcggct ctttgaagcc gatcaggatc
    17401 agcggaatga ggaacataac caccagaaca ccggctgccg ggatggcatg acctgtcata
    17461 aataagaaaa cgactgcaac gatcgcaccg tagaatacaa aaccggcgat accgtttgta
    17521 tcaaagaaga tgttctcagc atcttttctg cggactgcat tgataatatg cagaaccatc
    17581 gttgtcagga tcaggaacat accaaatgcg atggcaacaa cgaaagcggt gttcatagta
    17641 ccgataaacg gaagcgttgt cattgcatcc ttcggatgca tccagagtgc aggtatgaca
    17701 tcctcaaaac cgaagaaact gccgaacagg aaaccgaaga tcgtagagaa gactccggca
    17761 ctgcttaagc atgctgcgag ttccattttt ttgaaatggt agagcagggc accgccgatc
    17821 accaggcaga gtccctgacc gacatcaccg aacatggcac cgaaaataaa ggaataggtc
    17881 agtgctacga agatggtcgg atcgatctcg ttgtaagacg gaagaccgta catacgaacg
    17941 aataattcaa acggacggaa gataccagga tttttcaatt tggtaggcgg tgtcagtgtg
    18001 ctgtctttat cctgatcttc attgctttcg atcagacaga acagattctt gtcatcggct
    18061 acgtcttttt caaaacgagc ggcatcgctt tccgtcatcc agccacacag aatgtagaag
    18121 acctgtttac cttcttttgt gcaggctgca agtttacgca catcaaagtt tgaagtgagt
    18181 gaacggaatt tgtctctgca aagcaggact ttgtttttgt actcgttgag cagtttcgca
    18241 ctttcttcac ggcactcctg aagctggaca tcaagatcat ggatatcaga ctgaagtttt
    18301 gtaccgactt cctgtggaat accttcgtat tcatccggaa ggtggatgcg ttcaaagtgc
    18361 agggaagaaa atacggcatc gatctgtccg gctctgcccg ccggcacaaa gtaaacaccc
    18421 catacatact gctcatcttc gctgcagcga tagaacacgg tgtccagatt ttcgtagaca
    18481 tagtgctcca gttttttgta gtaatcgcgt gtgatcttac cgaaacgata tttgataaac
    18541 ttaaactgca gtacgctgtg aatatcaaaa tcgaatccaa ggaacggtct taaacgttca
    18601 taagaagctt tcagatcttc cttctttgct tccagagcat tggttcttgt ataaatatca
    18661 gaaaatttgc tttccagagt tgcgagagtc tcagaagctt cttcaatcga aatagtgccg
    18721 gttgggtttt cctgattttt atccgtaccg agcatggaag cgaattcttc tgcttttgtg
    18781 agcttgtctt tataaggatt gatctcaata taaggacgaa gagacttgac agtcttaagc
    18841 tcggaaagag cattttccag atgaatttcg tatttggata aatagtcatt caccacacgg
    18901 tcgatgtctt ctttcggacc ggtaacgctc agaaatttca ttttctcaat caacgattac
    18961 accccctttt cagatttgaa atatattttt gcgtctcact ggtcggaaga ccgtatcgga
    19021 tgcactcaag cgtggttgtg aggtcgtcaa cctcatgttc tttctcatag agatatgaaa
    19081 tgataactgc aatggaaaat ggatcctcct ttgcgttctt acgctgaaca cgataacgga
    19141 tctgataata ggcgtcgtcc agtgaaagtt ttgcttcctc cgggaaatgt cgtccgtagt
    19201 aagtatttct gatggctgcg gtcagttcat ctgtagttgc agcttcgacc atctctttga
    19261 tctgagaggt ctgaagacgg tattggatac cgatcagatg tgcatagacg tccgcattgc
    19321 tcatgttgta aaaatgcttg gaacgataaa tccaacgtat gttcagcaaa tccattttga
    19381 caccgtaatc gtccataaag attctttctt cgtcacgagt cagaaccttt ttgcggctgt
    19441 cccagaacca tttgaaatag cacaggtcaa gtgccatctc gtaatcccac aaagtaggtt
    19501 cggacagacc ggagagaaga gataaaggac gatagtagat gcttcctttc agaaggctta
    19561 caaactcatc cagagtttta cagttggaaa gagcggtcaa attgatgtcc gagtgttttt
    19621 caaagaaatc tttgaaaata tcgaaattca attcaacatc tctgtggtca aataccattc
    19681 gcatacaggt tttcagaagt ccgatctcgt atctgcggaa gtacagatcc aggattttac
    19741 gctgatgtat ggttgcgaaa cggtagatac tctggaaatc cgtgtagatt gcgtttgtga
    19801 gcattttctc gatctctccg cggtgcaggg aattttcgtt ggaatttgcg aaaataccag
    19861 cataagcgga ctttgttttc agaaatccga tggcatcgga aacggatgtc aaagaggcga
    19921 gatgctgaaa atctttagcg gtcagaagct tccttcgcat accgcgaatc ttggtcgctg
    19981 taccgctgta ggctaacaga tttcccataa ttttactcct cgatcatttt tgcgatcaat
    20041 ccggcagcaa gcttttcatg attctgattg taatcatttt cgattgcctg aatcgaatct
    20101 tcagttgctt tctgcagatc ggcaagactt ttttgcattt cttcatttaa gctggaacga
    20161 atctgatcaa gcttctcctg cgtagctttc tcagcctctt catcaaatgc ctgaatacgt
    20221 tcacgcatct gctgttccag tgccttcttt tcgttctcgg cagcctcaat gataccggct
    20281 gaggcctctt cgattgaaga cagacgcttg atgacgagat ccatgtgcat ccctccttta
    20341 gtatagtata gttgcagctt tgaaattcaa gctgaacata tgcaaaagca cagcccagcc
    20401 cacaaaaaaa ctgcagaagt ttattgccct gacacttctg cccttatatg attattgcca
    20461 gacaaccgta aaatcatgtt cacagatacc tattataaat aggtagttct tttttcactc
    20521 cgtctgacag cttacattat acacgatttc cacttgattt ccatatgcaa aattctcaaa
    20581 aaaataaaaa aattttatgg ttgttttgca caattagaat ggatatgata aaatacatgg
    20641 gtataactca gaagaaagtc agtgatacct agtatataaa atttatatga tgcggattgc
    20701 gagagtgcaa agcacggaac aatacgtatg gcatcagtta ctgacaaatc tcaatgtcat
    20761 aaatgccagg gtcaaaagcc tgaaagcacg atgtgcttgc acaaacataa aattggagga
    20821 aaataaatgc gtttaagaaa tataccggga gcggatgaag tgatcagaaa ctgtgactgg
    20881 gtaatcaagg aaccggcaga taaaaaagga aaatggaata caatatttgg aaatgaaaat
    20941 ccgatccata tcgaagttgg gatgggaaaa gggcagttta tcactgagat ggcaaagaga
    21001 aatccacaga tcaactacat cggaattgag cgttatacga gcgttcttct gcgtgccgta
    21061 cagaaaagag agcagattcc gacagaagaa gccctgccga atctgctcta tctgtgtatc
    21121 gatgccagtg aacttccgga aatctttgca aaagacgaag tggcaaagat ctacctgaat
    21181 ttctcagatc catggccgaa ggatcgccat gcaagacgca gactgccgtc aaaagaattt
    21241 ctggcacgct atgaccagat ccttgtgccg gatggcaaag tagaattcaa gaccgataat
    21301 cgcggactgt ttgattttgc actggaacaa agggagatcg ccggctggaa cctggacggc
    21361 tgtacctttg acctgcatca tgatgaaaac atgaatgaag gcaacgtgat gaccgagtac
    21421 gaagaaaaat tctcaagtat gggaaatcca atctataaaa tgatcatcag ccggtaaaca
    21481 gatgtcatat tatatagaaa cgaaatagaa accggactat ccgtttcgtt cggtggagga
    21541 aaaatggcac gaagacagaa tgttcgtgat cgcattggca tggcagtcta tgaaaagaaa
    21601 aagctgaaca agaaactggt gaccatgcag aaatcttttc aggaagtaaa gcacctgatc
    21661 ggggatgaaa aaaagaaaaa ataaaaagaa ggcgtttaca gtggagaatt tccggtgtaa
    21721 acgccttatg ttgtttcgga ctattttttg atgcggaacg ttttcgattt tccgcaggca
    21781 gccagcagat ttttacttta catcagtgct ttgtaccatc aaactcaaac ggctgcagat
    21841 gttccggaag tgattcgtca cgcttttgaa gctgatggtt gataagacgc tggatcagat
    21901 agcgtataaa tgcgactgcc ggaacaccga ggaacatacc aaggaccccg gcaatactgc
    21961 cgccgaccgt gatggcgaag atgatccaca gcggcttcag accggtagaa tctccgagga
    22021 ttttcggtcc gaggatcaga ccgtcaaact gctgtaatac aaataccatg acaacaaaga
    22081 tcacggcttt gagcggactg ataaaaagca ggatgatcgt acttggaatg gcaccgatga
    22141 atgggccaaa ataagggatc atgtttgtga ttccgacgat tacgctgatc agcacggcat
    22201 aaggaagtct caggatcagc atcagtacaa aacagagcac tccgatgatc gtagagtcga
    22261 tcgcctttcc gacgataaaa ctgctgaaca gacgattggc ttctgcagaa acttccagga
    22321 tcagctggat atattttacc gggacaaatg catagacaac agctttgcag gaattgacca
    22381 gaggacgttt gtctgccagc atataaatgg aaaccatgat cgcaataaag aggttcagca
    22441 cccatttaag gatggacatg gagatcgaat acagtgccgg tatcatattt gcggcaaaat
    22501 ccttcgcata gttgataaag ttcgggatcg cattgttgat cgtatttctc agcacttccg
    22561 tatccaacga agggaaacgc tcttctaaag tatcaaagaa atgataaagc tgctgtgccg
    22621 cagtcggaat aaaattgacc agatccgtca aactggtgat aatctgcggg ataacaccga
    22681 aaagtacgat cacaatgatg ccgatcacaa gaacataagc agtgatgatc gccaggatct
    22741 tacaaagagc atcactttta atcttacatt tatcacggaa cagcttgtaa aagcgggcaa
    22801 ctgccgggtt cagtatgtag gcaatcagag caccgatgat aaacggcatc agaacagcca
    22861 gacctttttc aatatttttc agtgtcgtgt tccaggatgt gatcagattg atgatgatcg
    22921 caccgaaaaa gatgacaaac atcgtataaa tacagattgt aaaataacgg ttattggccg
    22981 taaatttatt tttcttgatg tcatcatccg atttgttttg tttctccaga ttcatgggca
    23041 tcccccttat aaaatgttta aaaaacaagt tacttctaat tataaaaggc acggaaaatt
    23101 agttcaagtg ttctttctta aaaaaactat aaaaaaactt aaaaaaaagc ttgcaatttg
    23161 taaaactgta tattataata tgtcttgcgt tcgaggtaac gcaacacaag atatgcgcga
    23221 ttagctcagc tggcagagca cctgactctt aatcagggtg tccagggttc gaacccctga
    23281 tcgcgcacta taaaagcact gttttggatg acaagtagag agcatcgggg acggtgcttt
    23341 ttttcgtgca gtttgcacaa aagagaagtc ccacttgttg accgtggttt gtgttgagtt
    23401 caggaatcaa aaattggctg tgcttttttc tccggtgtac gcctttggac ttgttagaat
    23461 gattcgggtg tgttatatta gacatagaac tctaaaatca agtcgaacgt atgcgagact
    23521 tggcatgcga ttctggtcgg tttggatgat atttacttag cagaatcgat gtgcatttac
    23581 acacaataag acaggggttg atatcatggg attttttgac aaagctttaa aaaaggtaca
    23641 ggatgtggga gaaaacattg ccgcatcagc aaacaatgta aaaacagttg ttgagacaag
    23701 tgtacaggat acaatggaga tcaccggtct gaaaagacag atccgtgaat tagagaaaga
    23761 aatggatgca cagtatctgc agatcggaaa gaaatatgca gaatttgtag cagatatgga
    23821 tgatgcagaa ccggaaacag cagaaaatga agcagccgaa gcaacagaat cttcagaagt
    23881 gatcgatgaa gtgaaggaag aagcaattga cgaagtaaag gcagaagcag cagatgaaac
    23941 ggtcgatgaa gcaaaagaag aagcagctga tgaggacgaa gagccggtgt ttgatgtttc
    24001 tgatttcctg acgatcatca aacaggatca ggcaaagaaa aaggagctgg aaaatcagct
    24061 ggcggaagta gagaagagag caaaacagaa tacacttctt cgcgaaaaga caaaagcgga
    24121 agagtctttt gaacaggaaa aaggcgttct cgacaaagca cttgccatgg aaattatttc
    24181 taaggaagaa tatgagcaga agctgaatat tgccaggaaa aaagtagaaa attttgaaga
    24241 gatcaaaaaa atcgagcagc agttcgagat gggcattatc acaaaagaag aaaaagatga
    24301 gaaaataaat gccattctga atgcctgaca aatctggtgt ctgctttaca gaatcaaaat
    24361 caggttgtat aatcggggca gaatgtcgtc cgatggatgg acgaaatgga taaaggagat
    24421 gtagaagtta ttatggtaaa gattgatatt atttccggtt ttttgggtgc aggtaaaacg
    24481 accctgatca aaaaactgtt acaggatgca tttaaaggac agcaggtggt attgattgag
    24541 aatgaattcg gcgagatcgg tattgacggt ggatttttga aagatgcagg aatcgagatc
    24601 aaggaaatga attccggctg tatctgctgc tctctggtag gagatttcgg aaccgcttta
    24661 aaagaagtgg tagagaaata tcatccggac cgcatcgtga tcgagccgtc aggtgtcggc
    24721 aaactttctg acgttatcca tgcagttgaa aatctgcatc tggaagcaga cggagaagtg
    24781 aaactgaaca gtgccgttac ggttgtcgat gttctgaaat gcaaaatgta tctgaagaac
    24841 tttggtgaat tcttcaagaa tcaggtagaa gcagccggca cgatcatctt gagccgtacc
    24901 gatacaaaga aggcaacacc ggaaaaaatc gaagctgcca tcgaactgat ccgtgaactg
    24961 aatccggatg ctacgatcat cacaacaccg gtggaagatc tgggtggaca gaagatcctg
    25021 gacacgatcg aaggaatgaa gatcgacctt tctcatgtag aagaagagca tgaccattgc
    25081 tgtgatcatg accatcatca cgaccatgac catgaagagc atgagcatca tcacgatcat
    25141 gaggatcatg accacgacga gcatgagcat caccatgacc atggagaggg ctgcacctgc
    25201 ggctgccatg accacgatca tgacgagcat gagcatcatc acgaccatga tcatgacgag
    25261 catgagcatc accatgacca tggagagggc tgcacctgtg gctgccatga ccatgaccac
    25321 gagggacatc atcatcacca tcatcacgca gatgaggtat ttacaagctg gggtaaggaa
    25381 accgtccgta aatacagccg tgaagagatt gaaaagattc tgaaaactct gtcagaagaa
    25441 gattcttacg gaatgatcct gcgtgcaaaa ggtatgctgg agagcgaaga cggtgtctgg
    25501 acttattttg acatggttcc ggaagaagtg gatatccgtg agggaagtgc agaatacaca
    25561 ggacgtatct gcgtgatcgg ttccaaactg gatgaagcaa aattagagca gctgtggttt
    25621 gcgaaataag gaacaaggta aaaaaagatg agtgatttta atatttcaac accggtttat
    25681 cttgtgaccg gtgtcctgga gagcggcaaa acttcttttc tggattttac tgcgaaacag
    25741 gattactttc aaatagaagg aataacactc ctgatcacct gcgaagaggg tgaggaggag
    25801 tacgacgaga aagaacttct gaaatacaac acggttgtcg agaagattga agagccggag
    25861 gattttacat ttgagaacct ccgtgttctg catcgcaaat atcatccgga gcgagtgctg
    25921 cttgagtaca atccgctctg gggtgtcaac aaactgcgtg cgatgaaact gccgcttgga
    25981 tggggcattt ctcaggagat cgtgatcgta gacggcagca gctatcagat ttaccgtgca
    26041 aatatgcagt ctctgttctc agagatggtg cagaatgcag atatggtgat cttcaatcgc
    26101 tgtaaatcaa gtgatccgct gaccaattat cgaagaggca tcaaggttgt caatccttcc
    26161 tgtgatatca gctttgagga tgaggatggt gaactgatcg acctgtttga ggaaaacacg
    26221 ccgtacgata tggatgctcc gattatcgaa atagaagatg tggattacgg tatcttctat
    26281 gtggatatgg atgaccatcc ggacaaatac gtggggaaaa cggtgaagtt caaggctcgt
    26341 gccatgaaga gcaaaaacaa agaggcacct tattttgtgc cgggaagaaa agcgatgacc
    26401 tgctgtgcag atgatatgca gatgatcggc tacatctgta agagtccgat cgctccgcag
    26461 ctgaaacatg gacagtgggt tatggtcact gcaagggtag catttgaata cgataagttt
    26521 tatcgcggaa aaggtcctgt gctgtatgca agcaaggtag aaccgtgcga accgccggca
    26581 gatgaaatgg tatattttaa ctaagtaagc aaaacgctgg cgaaagccgg cgttttgtgc
    26641 gtttggtctg atacagcttc cggcggactg cagaggtgca ggatatcaga ttttttccgc
    26701 agattggggg ttttattatg agaatgtatg atctgatcag gaaaaagcga gatggggaaa
    26761 gattagcaaa agaagaaatt gactggatga tccgaagtta tacaaagggg gaaatcccag
    26821 attatcagat gtcagcactg atgatggcga tttattttcg aggaatgaat gaacaggaaa
    26881 ccttaaatct gacgcttgcc atggcggaca gcggagagcg gctggatctt tcggcaatcc
    26941 atgggattaa agttgacaag cattcgacag gaggtgttgg ggacaagaca tcgctggcac
    27001 ttgttccgct tgtagcttcc tgcggaattc cggtagcgaa aatgagcggc agaggccttg
    27061 ggcataccgg tggcacgatc gacaagctgg agagcttttc cggattttca acggcaatcc
    27121 cggttgaaac gtttataaag aatgtcaata cgatcggcat ttcgatcatg ggacagacgg
    27181 cagatctggc tccggctgac aaaaaactct atgccctgcg ggatgtgacg gcgaccgtgg
    27241 acaatctttc actgatcgcc agctcgatca tgagcaagaa actggctgcc ggtgccgatg
    27301 cgatcgttct ggatgtcaag accggaagcg gagcttttat gaaaagcgaa gaagatgcgt
    27361 ttgctctggc agaagagatg gtgacgatcg gcaaaaatgc agggcgtcag atgcatgcac
    27421 tgatcaccga tatggatcag ccgcttggtt atgcaatcgg aaatgctctg gaagtaaagg
    27481 aagcgatcgc aactttgaac ggcagcggtc cggaagattt cacggagctg gttctggcac
    27541 tcggaagccg gatgctgatc gccggcaaaa aggcagagga tgtcgcttcc gcagaaaaaa
    27601 tgctgcggca gtccatccag aatggagatg cacttagaaa actggaagca tttgtcaaag
    27661 cacagggcgg cacaggagaa gaagtttctc atccggagaa actgcctgca gcctctatgc
    27721 agattgcagt accatcgccg cagagcggct atatttctca tatccagtgt gatgaaatcg
    27781 gagtctgctc cctgcttctt ggcggcggaa gagagacaaa agacagtgtg atcgaccttt
    27841 cggtaggact ggagctgaaa aagaaagtcg gagattatgt cgaaaaaggc gagacactgg
    27901 cggttctgca tgcaaatgat caggaaaaag ccgggcaggc agaagagaga ttcctgcgtg
    27961 cataccggtt ttccgagacg caaccaccga aacgtccgat gattttcgga gaaatttaaa
    28021 agaatttcaa ataataccta tgaaacatac tgaaattatg gtataaagaa agcataatgt
    28081 aaaattgagt gtcttactct gcaaaaagca atttgtataa ctttaccgca gagaagcatt
    28141 cccccgcttc aactgcacgg agcactaagc ggtggcagga gtgaaaatct gctcaggagt
    28201 atctttatga catcatttgt ttaaaacaaa agaataaata atatgaggag aacaagtatg
    28261 gaacagctga ttcaggaatg gagagcatct cttcccaaat tcagggagat gacagaaaaa
    28321 ttttatgcga aagaagtaag cgtgaaagaa tacaaaggat tttccggagg atacggaagc
    28381 tatgcccaga aaggcggcaa ggcaagcatg ctgcgtctgc gtatgccggg cggaagactg
    28441 acaaaagaaa aactgaaatt tatcgcagag atggtagaac agtatcatgt ggacaaagca
    28501 cattttacga cctgccaggc agtgcagctg catgatctgg acaaagacag cgtctgtgat
    28561 atcatggaaa aagcagtgga tgtcggtatc atcacaaaag gcggtggcgg agatttcccg
    28621 cgaaatgtga tgtgttctcc gctttccggc gtagaaatgg gagaatattt tgacgtactg
    28681 ccgtatgcag aggctgtgtc tgattatctt ctgaaaaacg cagataaagt gaccatgccg
    28741 cgaaaactga aagtcggatt ttccaattct ccggccaatg tgacacatgc gaccttccgt
    28801 gatcttggat ttgtagcagc agaggacgga acatttaccg tatatagtgc gggcggactg
    28861 ggcaacaatc cacgcatggg tgtgaaagtg gcagaaaacg tagagccgtc acagatcctg
    28921 tattatgtgc aggcgatggt caataccttc ctggcatacg gaaactatga gaaccgcgga
    28981 aaagccagaa cacgttatat gcaggaaaaa ctgggaagcg aaggctatgt gaaggcattt
    29041 ctggagaaac ttgaagaagt aaagaaaaat gaaaaactgg atctgaacct tgctgtttcc
    29101 ggtacagaaa aagcagccga cggtgaactt cagacagaaa acaaacgaat cgtggcacag
    29161 aaacagccgg gactgtatgc agtaaaatat catccgatcg gcggtgtgcc gaaagtgtca
    29221 aaatttggcg agatctatga aagcatcaaa gatgtatcgg atgcagaaat ccgcatttca
    29281 ccggatgaga cagtctatat catcaacctg acggcaaaag aagcagaaaa agtgcttgca
    29341 gctacggatg acggagcaga aactttattc gaaagttccg tatcctgtat cggagcaacg
    29401 atctgtcagg ttggtgtgcg tgattcccag ggacttttaa gaagactggt tgagacagca
    29461 aaagaatatg attttgcgga cggtgtactg ccgcagatcc atatttccgg atgtacttca
    29521 tcctgtggga cacatcagat cggaaagatc ggtttccacg gcggagtaaa gagaattgac
    29581 agtgtgacag agccggtatt tacgcttcat gtgaacggct gtgacagaca gggtcaggag
    29641 gcatttggcg aatcctgggg catgatcaaa gaaagcgata tcccggactt tctcatggaa
    29701 gttggaaaaa ccgttcagca ggaaaacacc gtgtttgcca catggtatga aaagacaccg
    29761 gagaaattaa aagagattgc tgaaaaatat ctgattaatt caagtcgaac gcacgtgaga
    29821 cttggtgcgt gattctggtc agcgaaagct
//
LOCUS       DAWDWH010000004        29832 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_907_length_29832_cov_17.248984, whole genome shotgun sequence.
ACCESSION   DAWDWH010000004 DAWDWH010000000
VERSION     DAWDWH010000004.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 29832)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 29832)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29832
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_907_length_29832_cov_17.248984"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4960..4969
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7468..7477
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15696..15705
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8934 a         6048 c         7294 g         7526 t
ORIGIN      
        1 gtctgatctt gtgtcagctg tcttatttta aatttcagga tctggtgccg gatcttgtcg
       61 aaaaaatgga tggaaatgac atcagaggag tgacgatgcg ttctcttcgc aaacatccga
      121 aatacaacag tctgtatgtg tcagactggt acgaaaaaga caatcgcagg ctctatgcgg
      181 cggttgccag aagcaggcgt tttgcaaaaa tgcggctcaa ttattacacc aatctcacgg
      241 acaaagaaat gcagatgcag ttctcggcag ttacatttct gctggaagac ggaacggtgt
      301 atgtggcatt tcgcgggaca gatgagacga tcgtcggctg gagagaagat tttgatatgg
      361 cactgaaaag cccgattccg tcgcagacgg cggcaaggct ctatctgcgg cacattgcag
      421 aatacaccgg cgatgccccg ctgatgattg gcggacattc caaaggcggc aatgaggcag
      481 tctttgcagc gatggagaca gaaccgcaga tccaggaccg gatcagagct gtatactgtt
      541 ttgacgggcc gggtttccgg gaagatattt accggaaaga aggttatctt cggatcgaaa
      601 agaagatcat caaaatggtt ccgcaggatt cctttgtggg catgcttctg catactgcgg
      661 gaagttatca ggttgtggaa agttccggga aaggtgtgct gcagcatgat atgttcacct
      721 gggtagtaaa agaaggggat tttgtataca aagaagagat taacccggcg accgaaaaga
      781 aaaatcagca gatcaatgag tggatcgcat cgtattctcc tgaggagcag caggagatcg
      841 tggatgccct ttttgagatc attgaagcaa ctcaggcaga cacggttatg gattttacgc
      901 agaaccgttt acagaaaatg atgaagatgc tgaacatttt tcatggtctg gaggcaaaaa
      961 caaagcgaaa tgtacgcaag cttttccata tcctgcttgc accgtcggaa agattaggat
     1021 atactgagag cagtaagaaa acggcagaac cagattctgc tgtaaaaatg gaaaacaaag
     1081 gagtttataa aatggaattg gtaacagtaa gagaaattta ccgtgatacg gaaaaatatt
     1141 taaatcagaa gattacagtg ggaggatggc ttcgaagcgt cagagattcc aagacatttg
     1201 gatttttagt gctgcatgac ggtactttct ttgagacact gcagatcgta taccacgata
     1261 aaatggaaaa ctttgcacag gtttccaaac tgaatgtagg agcagccgtt atcgttaccg
     1321 gtacgctgat cccgacaccg gaagcaaaac agccatttga gatccaggca gacgaagttg
     1381 tggtagaagg agcttccgca ccggattacc cacttcagaa aaagcgtcac agctttgaat
     1441 atctgcgtac tatctcacat ctgcgtccga gaaccaatgc gttccaggca gtgttccgtg
     1501 tacgttcact gaccgcttat gcgatccaca aattcttcca ggagagagat ttcgtttatg
     1561 tgcatactcc gctgatcacc ggaagtgact gcgaaggtgc gggcgaaatg tttcaggtaa
     1621 caactatgga tctgaacaac atcccgaaaa cagagcaggg cggcgtagat ttcagccagg
     1681 atttcttcgg aaaacagacc aacctgaccg taagtggaca gctcaatggc gaaacctttg
     1741 cacaggcatt ccgcaacatt tatacattcg gaccgacttt ccgtgcagag aactccaaca
     1801 caacacgtca tgcagcagaa ttctggatga tcgagccgga gatcgcattc tctgatctga
     1861 aagatgacat gatccttgca gagtccatgc tgaaatatgt gatccgctat gtcatggaaa
     1921 atgcaccgga agaaatgcag ttcttcaaca attttgtcga taaaggactg attgaccgtc
     1981 tgaaacatgt agtggaatcc gagtttgcac acgttactta tacagaagca atcgaattgc
     2041 ttgagaaaaa taatgataaa tttgaatata aggtatcctg gggatgtgat ctgcagacag
     2101 agcatgagcg ttacctgact gagaaaatct tcaaacgtcc ggtatttgta acagattatc
     2161 caaaggagat caaagcgttc tacatgaaac tgaacgaaga tggaaaaacc gttgctgcta
     2221 tggactgtct tgtgcctggt atcggtgaga tcatcggcgg aagtcagaga gaggacgact
     2281 acgaaaaact gctcgcacgc atgaatgagc tgggactgaa ggaagaagat tacggattct
     2341 atctggatct gagaaaatat ggatctacaa gacatgcagg tttcggactt ggattcgaac
     2401 gttgcgttat gtacctgacc ggtatgtcaa acatccgtga cgtaatcccg ttcccgagaa
     2461 cagtaggaaa ctgcgaactt taataaaagc atagtcttac aggcggcacc cggttttctt
     2521 gacagagccg ggtgtctgta atcttgggtg gagttcagac tcttgcacga ttaaggcttc
     2581 gcagaaccgc ataccaggtt ttgcctgtgt tcgacttgaa aaatgggtgc aagctgtatc
     2641 aggaggaaag tacagaaatg ggaaaccgaa ttttagtggt tgatgacgaa aaggaaatcg
     2701 cagatgttat tgaactgtat ctgaaaaacg aaggctttga ggtcgaaaag ttttatacag
     2761 gaaaagatgc acttgtatcg attgagaaag cagcaccgga tatggcactt ctggatgtga
     2821 tgcttccgga tattgatgga tttgaggttc ttcagaaaat ccgtgaaaaa tataattttc
     2881 cggttattat gctgacggca aaaacagaat atatggacaa gatcaacggt ctgatgctcg
     2941 gggcagatga ctacatggca aagccattta atccgctgga actgatcgca cgtgtcaaag
     3001 cacagatgcg tcggtttacc aagtacaata caggcggaag ggcacaggaa gatgtgattg
     3061 attttggaaa tatgtttctg aaccgtacca cgcatgagtg tatttacaat gaaaagcatc
     3121 tgactttaac accgattgag tttgatatcc tctggctgct gtgtgacaac cgcgggcagg
     3181 tcatcagttc tgagcggctg tttgaagaag tctggaaaga aaaatattat aaaaacagca
     3241 acaatacggt tatggtccac atccgtcacc tgagagaaaa gatgagcgga ccgaccggca
     3301 agagtaattt tatcaagacc gtatggggag tgggataccg cgttgaaaaa taaatatcgc
     3361 aggctgaaat ggaaaatgat gctgcagacg atgggcatta tgatgatgag cctggtggtc
     3421 ggaaaaatga tcatcacact gatccttgac ggattgtttc agggaaagct ctcaaacctg
     3481 atcatcggta ttctgatgaa aatgaatctt tcgcaggcag aagcagatgt gatctatcag
     3541 aatatatttc gggcaaaccg ggatgtgttt cagtggatcg gatttatgct gctgtttctg
     3601 gtcggatatt atgcggtact gtcaaagact gctaattatc tgaaaaacat cggagatggg
     3661 atcgacaatg ttttagcaaa ttcaaaacag ccgatcgagc tggaaacgga actggagccg
     3721 atcgcagaga aattaaatac catgaaaatg acgctggcac ggaaagaacg catggcacag
     3781 gaaagtgagc agcggaaaaa tgatctggtc gtttatctgg cacacgatct caaaacgcct
     3841 ctcacatctg tgatcgcgta tttaagtatg ctggacgaaa aaccggatat gccgccggaa
     3901 gaacgcaaaa aatatatcca tatcgcacac accaaggcga tccgtctaag tgaactgatc
     3961 agcgaatttt ttgagattac aaagttcaat cttcagaata tccgtctgga gaaagagacc
     4021 atcaatcttt cactgatgct ggagcagatc atggatgaat tttatgccgt gtttgccgat
     4081 aacaatctga ctggaaatat ttatacggaa gaagatctta tggtagaagg tgatccggac
     4141 aaacttgccc gtgtgtttga caaccttctg cgaaatgcgg ttgcctatag ttaccgcggc
     4201 acaaacattg atgtgcgtgc atacggggaa ggtgttgccg ttgtcatcgt attttctaat
     4261 cagggagagc cgatcccgaa acagaaactg cagaccgtat ttgaaaagtt ctaccgtgcg
     4321 gataattcca gatcgagcca gaccggagga gccggacttg gactttcggt ggcaaaagag
     4381 atcgtggaat tgcatgaagg aacgatcgaa gcctttagtg acattcagtc cacccgtttt
     4441 gtggtgcgtc tgaagcgact tttgccgaag gggcagcggc aatcgggagg tgttatttaa
     4501 atggaagaaa acaagaaaaa gaagcgtacc gccggagatg tgatccggac gctgatcatg
     4561 atcgtggcac ttggcgtgtt ctgttattcc ggctatcagt tgctttcgat ttatcaggaa
     4621 tataaaaagg gcagtgatga gtatcatgca ctggagaata aatatattga tgaaaagggc
     4681 atgtttgaag agatcaacac ggatgcggcg gagccgacga tgaaaaatcc gatcgatttt
     4741 gccggactga aagccgtgaa tgaggatatt atcgcctggc tgagggtcgg tgcgatcgat
     4801 atcagttatc cggtcacgca gggcaaggat aacgattact atctgcacaa tacctttgaa
     4861 aatcagccga atattgccgg atgtattttt atggatcacg gctgcaaaaa agatttttcc
     4921 gatccgaaca cgatcattta cggacacaat atgcgaaatn nnnnnnnnng tgtttgactc
     4981 ggatgcatat ttctgggtat atacgccgga gaagatttat aaatacgaaa ttttctcctg
     5041 ccaggaagtc ggtgcgacaa gcgaaaccta tcagcttcag ttttccgaca aaaagaaatt
     5101 tcaagaatat atcgattcct gttttgaacg atccgttttg aaacgtgaca tcgaagtgac
     5161 aagagatgat aaaatcgtga cactttctac ctgtaccgga aattccgaga caagatttct
     5221 cgtgcagggc aagctgatcg aaacctataa ggcagtgtaa gagattttgc ctctggcatc
     5281 atataatttg ggtatgaaag ggtgatttgt atgaatggca cagaaaaact gaaagaactc
     5341 atcaatggaa gcaataatat tgtatttttt ggcggggccg gtgtctcgac agaaagcggg
     5401 attcctgatt ttcgaagtac ggatggactt tatcaccagg agtatgatta tccgccggaa
     5461 accattctga gccatagttt tttcatgcga aacccggaag aattttatcg tttttacaaa
     5521 aataaaatgc tctgcctgga tgcaaagcca aacatggcac ataaaaagct ggcagagctt
     5581 gaaaaggccg gaaaattaaa ggcagtcatc acgcagaata tcgacggact gcaccaggcg
     5641 gcaggaagca aagaagtgct ggagcttcac ggaagcgtgc atcgcaatta ctgtatgaaa
     5701 tgcggaaaat cttatgatgc agaatatatg cttcatgcag aaggcgtgcc gacctgttcc
     5761 tgtggcggaa ggatcaaacc ggatgtggtg ctctacgaag aagggcttga tatgaccgtc
     5821 atggcaagag ccatccaggc gatccaggat gcagatatgc tgatcatcgg cggaacatcc
     5881 ctgatcgtct atccggcagc cggactgatt gattattaca gaggaaataa actggtcgtg
     5941 atcaatatga gtgtcacatc gcaggataaa aatgcagatc tggtgatcca ggaaccgatc
     6001 ggaaaagtat ttgccggcat ctgaacatat aacgatctgt aagaatatat gagatcattt
     6061 tgtagattgg gagggacagg atggattatg aagtaggcga tatcgtgaaa ctgaaaaaac
     6121 agcatccgtg cggaagtaaa gagtggagga tcctcagggt aggagcggac ttccgactgg
     6181 agtgtatggg atgttcacat cagatcatga taccgagaaa acaagtggag aaaaatacca
     6241 gagggcttcg caagaaggag taggacttgt gaacgtgcaa atgaacgaat tctccgaagg
     6301 cacttttacg tgcccttcgg agaattcgtt catttgcaca tgcatatttt tggaagccag
     6361 cactttataa caccttcatc ccattctttt acactgaaaa agaaaaaacg ataatttttt
     6421 ttatcttttt ttagaaaaac tttatgtgta tatccaacag cgatatgcta tactgaaagg
     6481 gatttaaaac cgaggcaagg agcgattttt atgaaattaa aaacgaggct tatcatagcc
     6541 ttttttgtta ttatctgtgt gcctcttttg cttgcaggag tatcgctggt tggtttttct
     6601 acgtatcaga tccgtaatct ggaggatcat ctcggcattt ccggtgtgac ttatgaaaat
     6661 atgtcggatt cggtgcaggt tctgaacaaa gcgacgaact ccaattttga acagatcaaa
     6721 gcaaagggca gtgaaaatcc ggatgcattt ctgaatcgtg attatctgga tcagctgaat
     6781 gatgagctga aagagaaata ttcttttctg atcgtaagaa agggcgacag cctttattat
     6841 tccggaagtg atgagagcgt gaataagctg tttccggagc tgccggagta tagtgacgtg
     6901 gtcagccagt cggacagcgg cgtttacatc ggtaaggatg tgaaggcgtt cgtcaagcag
     6961 atggatctga cgttttcaga tcagacagaa ggcagtgttt ttgtaatcac cagcctgacg
     7021 atggtgcgtc agataaggac tcttacaaga gatatgcttg tttctatcgt gatcattctg
     7081 atcatgacag caggtatgct gacgatgtgg atttacaatg gaatcaacat tccgctgaaa
     7141 aagcttaagg aagcaacgca gaacatcaaa gagggaaatc tggattttgt tctggatgca
     7201 gaaggtgacg atgagatcag tgagctgtgc cgcgattttg aggaaatgcg gcagagactg
     7261 agggagtcat ctgaggaaaa gatacagtac gacaaggaaa ataaagagct gatcagcaac
     7321 attgcacacg atctgaaaac tcctatcaca gccctgaaag gttatgcgga ggggatcatg
     7381 gatggtgtgg cggatacgcc ggagaaaatg gaccgttaca tccgcacgat ttataataaa
     7441 gcaaatgata tggatcgtct gatcaatnnn nnnnnnnagc tgacgttcta ttcaaagatc
     7501 gatacaaacc gtattccata tacattcaat aagatcaacg tccgtgaata ttttgatgac
     7561 tgtgtggaag aggtcggact ggatctggaa tccaaaaata ttgagctgtc ttactttaat
     7621 tcagttgaaa atgatgtgct tgtgatcgcc gatgcagagc agctgaagcg agtcattaac
     7681 aatattgtcg gaaactctat caaatatatg gataagccaa aaggctatat cagcatccgt
     7741 gtcaaagatg tgggtgattt catccaggtt gagatcgaag ataacggacg aggtattgcg
     7801 gcaaaagatc tgccggcaat ctttgaccgg ttcttccggg cggatgcatc caggaattct
     7861 tccaaaggag gaagtggaat cggtctttcc atcgtacgca agatcatcga agatcacggc
     7921 ggaaagatct gggcaaacag taaactcgga tgtggtacag tgatgtactt tgtccttaga
     7981 aaatatcagg aggtgcctgt aaatgagcag aatactaatc atagaagatg aggagagtat
     8041 tgcagagctg gagaaagatt atctggaact gagcggattt gaagttgaga ttgagagcaa
     8101 gggagacgtt ggactcaaac gtgccctgga agaagaattt gatctttaca ttctcgacct
     8161 gatgctgccg ggaatggacg gatttgagat ctgtaaagag atcagaaatc acaaaaatac
     8221 gccaattctg atggtttctg caaagaaaga tgacattgat aagatccgtg gactgggact
     8281 tggagcagat gactatatga ccaaaccgtt cagcccgagt gaactggtag cgagagtcaa
     8341 agcacatatg gcacgttatg agcgtctggt cagcacaaat atgccgaaga atgatatcgt
     8401 agagatccgc ggcatcaaga tcgataagac agcacgccgt gtgtgggtaa atggagaaga
     8461 aaaagcattt accacaaaag aatttgatct gctgaccttc cttgcggagc atccaaacca
     8521 tgtattcaca aaagaagagc tgttcagtga aatctgggat atggagtcga tcggagatat
     8581 tgcgaccgtt acggtacata tcaaaaagat tcgtgagaag attgagttta acacagccaa
     8641 gccacagtat atcgagacaa tctggggcgt gggatatcga tttaagattt aaagatgcca
     8701 gggtcagaag catgaaacca cgatgtgctt gcacaagagt agttttatgg catcgcttaa
     8761 ccccagtatt tacaaggagg aacatcatga aggaagtaaa aactccaaag aagccgttta
     8821 tctattatct gatcattgcg gcaacgatca tgatactgct caacacactt gtttttccga
     8881 atgtgctgaa gcatcaggta aaggaagtcg gctacaatgt ttttatgcag atgacaaata
     8941 atcaggagat cggctcggta aatatcaccg attctgagat cttctttaca aacaaagaca
     9001 attcgcagat gtacaaaacc ggcgtgatca acgacccgga actggtagac cgtctgtaca
     9061 aagcaggtgc aactttttcc acggagatca cagaagaaat gtctccgatc gtttatcttc
     9121 tgctcggcta tgtactgcca atctttattt ttgttgcact tggcagatgg ctttccaaaa
     9181 aaatgatgag caagatgagt gacggggcaa acccgatgaa ctccatgatg tttggcacaa
     9241 agagcaatgc caagatctat gtaaaatcca caagcggcat caaattctcc gatgtggcag
     9301 gagaggaaga agcaaaagaa ctgcttacag agatcgtaga tttccttcac aatcccggca
     9361 aatatcagga gatcggagca tcgatgccaa aaggtgcact gcttgtaggc cctccgggaa
     9421 ccggtaaaac attgctggca aaagcagttg caggtgaggc gaacgtaccg ttcttctcga
     9481 tctccggttc tgagtttgtt gagatgtttg tcggtatggg tgctgcaaaa gtgcgtgacc
     9541 tgttcaaaca ggcaagcgag aaagcaccgt gtatcgtctt tatcgacgag atcgatgcgg
     9601 tcggcaagaa acgtgacgga cgcatcggcg gcaacgatga gcgtgaacag acgctgaatc
     9661 agctgctcac ggaaatggat ggatttgacg gttccaaggg tgttgtcatt ctggcagcga
     9721 ccaaccagcc ggattctctt gacccggcac tgctccgtcc gggacgattt gaccgtcgta
     9781 ttccggtgga actgccggat cttcagggtc gtgtggatat tctgaaagtc catgcaaaaa
     9841 agatcaaact tgcggataac gtagatttca atgcgatcgc cagagctgca tccggagcat
     9901 ccggagcgga acttgcaaat atcgtgaatg aagcagcact tcatgctgta cgctgcggac
     9961 gcaaatttgc aacacaggca gatctgatgg agagcgtgga agttgttatc gcaggatatc
    10021 agaagaaaaa ccgtatcctg tcggataaag agaaactaat cgtttcctat cacgaggttg
    10081 gtcatgcact ggttgctgcc atgcagacca attccgcacc ggttcagaag atcacgatca
    10141 ttccgagaac ttccggtgca ctcggctata cgatgcaggt agaagagggc gagcatttcc
    10201 tgatgaacaa aacagagatg gaaaacaaga tcgcaacctt taccggcggc cgtgcggcag
    10261 aagaactgat ctttggtgag atcacaaccg gagcttccaa tgatatcgag caggcaacga
    10321 aatttgcgag agccatgatc acacgttacg gtatgaacga agagtttgat atggtagcca
    10381 tggaaagcgt ttctaaccag tatcttggcg gagacagctc tttgacctgc tccatggaga
    10441 cacagacgct gatcgaccag aaagtcgttc agctggtcaa agaacagcat cagaaggcaa
    10501 aaaaaatctt ggaagacaac gttatgaagc tgcatgagat cgcaaaattc ctttacgagc
    10561 atgagacgat caccggagaa gaatttatgg agattctgaa cagcgacagc cagaagatca
    10621 tcgaagagaa acagcagtaa aaaagaaaca caaataaatg aaagaaaaaa atccggaacg
    10681 gacagtaaaa actgttttgt tccggatttt ttttgcggca ggatcactgt atatatctgc
    10741 ggagtgattt cgttgaaaat actgctccgg ttaagatgca aaacatacag tttcagccag
    10801 tttctggagc ttgaatgcag tcatcacatc aagagttccg gtatagggga tgcccattga
    10861 ggatctcgaa aaaacagtgt tgtaaatgac gcatgctgct aggtaacttc cttctacggt
    10921 cgggtgcgaa tagtcaccca tcagaagttt gatcccatac gaagaatggc tgcagagcag
    10981 aaatgctgtg ccaaccggag cgacgagagc attgtgctct tcggcgatct ccgcaatcgt
    11041 acggttaaga atactctggt aatcagagcg gtttgacacg gaatcgtacc ggttataatc
    11101 attatgccat ttccctgggg cccatgtctg ataaaggacc atctttgcac cgctggcctg
    11161 aatataagga gccagcttgc ggatggttct tttcatacgc tgtggtttgg aaatcggata
    11221 atagcgtcga tcctgcaaaa tgacgtaatc ccatcgctgc atacgcagaa gccggtaaag
    11281 ttttctgccg ttggcagttg tagggttgct tgtatttttc agggaacgtc ccggattggt
    11341 gagtgacctt acgatcacgt tctgcccaga actcttgcac agtttccgaa aaatatttgg
    11401 catatgattt cgctgtgtat agctgtttcc cacaaataaa atgcgtgtgg gagatgttgg
    11461 tgcttccctg tgccctgcgg catgtgctgt gacaggaatc cagcacagca tcatggcaag
    11521 acaaactata aaaatcgatt ttttgatcgg taaaaacttc tgcatcagaa ttcagcagct
    11581 gtctgcagga tcagaacggc atcggaagtg tctgcaatac cgtcctggtt catatctgca
    11641 cttacggttg cagcatcgtc cagagtatca agttcagcgg cagaacgaag cagcagtgca
    11701 ctgtcgtcag tagttacgct gccgtcgtta tttacgtcgc caagcagacg aacgtttgca
    11761 actttttcgg tcagcaaggt aactgcattg ttgattgcat cctgaagtgc atccgggttg
    11821 tcattcactg cttttccggc agccagagct tcttccagac cgttcagtgt agaagataca
    11881 tagctgtctt ttgcagcaac gatggcttct gcttttgcga tgacagcaac cagagcagct
    11941 ttgtttccga cacgttccag attagtgatc gcagtgatga tatcagcata tgccttgccg
    12001 atcgcctccg gtgcacggtt caggtcatca atgacgtctt ttgcagcatc cattgcatct
    12061 ttcagatctt tatagctgtc ggatgtaaag tctttttctt ccagaccgcc tgcggaagca
    12121 accagttttt tcagagcggc gaggtcagct ctttcagcca tagcagccag tgcatccagc
    12181 agtgtggaag cagcacggtc aacttctgtc tgagtagcag atgtgtttgc ataaacagct
    12241 tttgcagctg tcagagcagc tgtcagatct tcggtgtttt cataatcagc agcacgttcc
    12301 agaagcttgt cagcagcatc gatggcaacg ttcagatgtg ttttctgaac accgtatgcc
    12361 aggttgttga ctgcatcgac cagagcggac acagcactgt ccatttctgt gatggaagca
    12421 ttggatttgt caagaacagc ttttgcagca gttaaagcac tcttcagtgc agctgcggaa
    12481 tctgctgtat aaacagaaag atcagcgtct ttccatgtgt tgtagaagtt cagcagtgag
    12541 ttttcaactg cataatagga acttggttcg actgcatcgt aagtcagacc ataaccgaaa
    12601 gcgtatttga tatcggatgt catctgacca ttttcgataa ctggaacatt aaccggaagt
    12661 ttaccggatg cagcgaagcg accaaagata acttctacgc cggcgatgat gttcggacca
    12721 aatgcattat ccggtttcag agcttctgtc ggatccatac ctttgtttcc gtatacagct
    12781 acgatcgcat cagaatcagc atagtttgca acatcataag gtttggaaat actcataacg
    12841 atggatttct tgccctgtgc ttttgcgtat tctgtgatag cggtcggctg tgtggtcagc
    12901 cagttggaaa agtttgtgga aacttcggaa ataacgatca cgtagtctgc atcgtcaatt
    12961 ttctgtttta cctggtcaag gtttgcagaa gtgtaacgaa atgtctcata atctactttt
    13021 ccgctgatga tgtgatctgc gatcacacgt ctcataccaa gtgcgagtcc cggagtttcg
    13081 ttgttgtatg caccaagcag aagcactttc tgtcctgcct gcggtttcag cggcagaata
    13141 ttgtcgttat ttttgataac ggtaacagcc tgggcagaaa tatttctctc aatgtcacga
    13201 ttctgttcag aaccaacctg ctcgtttgca acggcaagtt tttcctcgta agtacgagta
    13261 tcgcttgtgt aattcaggat gcctctcttt tctttcagag aaaggattct cttaacagat
    13321 tcgttgatgc gatccacaga gatctctccg gaattaacag cagcttctac gccgtccagg
    13381 atcgcatcca ttttcggcat atctgctttg cttctgagga tagtcggcat caggcaaatg
    13441 tcaacaccgg ctttgatggc acggatgcag gcatcggttt caccgaagtt ctgagaaatc
    13501 gcatccatgt tcagggcatc ggttacgatg atgccgtcat agtgcatttc gtttcttaca
    13561 aggtcagtaa gaatggtttt ggaaagcgtt gccgggagac tgatctcgga accgtctttc
    13621 ttggaaatgg cagtttcttt ttcaacctgt ggatactgaa tgtgtgctgt catcagcatg
    13681 tctacaccgt tgtcgatcat tttctggaac ggaacgagtt cacactgtct gagttcgtcc
    13741 agagatttgt ctacacaagg aagacctgtg tgggagtctg tagcggtatc gccatgaccc
    13801 gggaaatgtt tggcagcagt agcgatgttg tacttctgca tacctttcat ggcagcagtt
    13861 gccatgcttc ctaccagttc cggatcgctt ccgtaagaac gaaggccgat aaccgggttg
    13921 gaaggattgc tgttgacatc ggcaacaggt gcaaagtcta cgttgatgcc gagggcactc
    13981 agctcgcgtc cgatcacttc tccggactgt gttgcagcgt ccgtgcttct ggttgcaccg
    14041 agagccatgt ttccaggaag agctgttccg cttccgagac ggtaaacaat accgccttcc
    14101 tggtcgattg tcaggagcag cgggatgttg ctgccgtcgg ttccgcctgt ggcagcttcc
    14161 tgaagatccg ttgtcagttt cagtgtctga tctgtctgag ctacattttc agcgaaaaga
    14221 ataactccgc cgaaatcata atccttgatg atctgagcca cttcatcgtt catcacctgg
    14281 aaatttttct gtcccgagtc cgattctgtc tgccatttac ggaaatcagg catcatcatc
    14341 tgggcgatct tctgacggag cgtcattttg ctcatcagat tgtcgacaac agaggtttca
    14401 gcagccgggt cagcaggagc agcatttaca gctaatgttc ccagtgagct gacacacata
    14461 acggctgtca ttgcagaagc cataaagcgt ttgagtgtgt ttttcttcat ttcttttctc
    14521 tcctttcata agataaatat taaagatgtt gagaataaaa atgatgccgg cgaattgctt
    14581 cgttataaag tgatggaccc tctcactctg gcatcataac tcaaatttat catagcatat
    14641 ttaactgaaa attcaatgat gatatatgaa atatttagta ccttttggca aatataagat
    14701 tgagaaaatg acaaatagaa aaagaatatg tttgtcaggc aactcttata gctgaacaac
    14761 ttttttgcct tgacttttcc ggaatttcta ctatataata gggacagact aacaggaaaa
    14821 taacgcagaa gaagacaagt acgtctgtcg taaaagacac agagagctcc ggtgggtggg
    14881 aaggagtaca agtaacacag gcggaatatc acttcggagt tgcagactga aagctgtttt
    14941 atagcatcgc tgaaagtaag tcgtgccgga ttcccccgtt atcgggaacg catatcgcat
    15001 tttgtcggta tgttgtaaca ggtggttatg agagtattgg ctgtcatcct tttacaggat
    15061 ggcagctttt tgatttattc cttataataa ggacaggagg ttgcttatgt acgaaaaagt
    15121 ttcgacaaat ctgaatttcg tagacagaga aaaaaatgtt gagaaattct gggaagaaaa
    15181 tcacatcttt gaaaagagta tggaaaaccg taaagaaggt gaaacttaca ccttttatga
    15241 cggaccgcca acagctaacg gcaaaccgca tatcggacat gttctgaccc gtgttatcaa
    15301 agatatgatt ccgagatatc agaccatgaa aggcaaaatg gttccgcgta aagccggatg
    15361 ggatacacac ggtctgccgg ttgagttgga agtagaaaaa atgctcggtc tggacggaaa
    15421 agaccagatc gaagaatatg gactggttcc tttcattgat aaatgtaaag aaagcgtatg
    15481 gaaatacaaa ggcatgtggg aagatttctc cggaacggtt ggtttctggg ctgacatgaa
    15541 aaatccgtat gttacctatg atgataacta tatcgagtct gaatggtggg cactgaaaac
    15601 catctgggaa aaaggcctgc tgtacaaagg attcaagatc gttccttact gcccgcgctg
    15661 cggtactccg ctttctgccc aggaagtggc acaggnnnnn nnnnncaggg ttacaaggac
    15721 gtaaaagaac gttctgctgt tgtacgtttc aaagtcaaag acgaggacgc ttacattctg
    15781 gcatggacaa ccacaccatg gacactgcct tccaaccttg cactctgcgt aaacccggat
    15841 gaggattatg taaaagttca gatgaaagaa gacggccgtg tttattacat tgcacaggct
    15901 ctggctgaca aggtgctcgg cgaagatgct tacaatgtac tggagaatta caaaggaaca
    15961 gacctggaat ataaagagta cgaaccactg tacagctgtg cagccgttca tgcagaaaaa
    16021 cagaacaaaa aagcatttta tgttgtttgt gatacttacg taacactgac agatggtacc
    16081 ggtatcgttc atatcgcacc ggcatttggt gaagacgatg cgaaagtcgg acgtaaatac
    16141 ggactgccgt ttgtacagct ggttgacggc aaaggtaacc tgacggaaga tacaccgttt
    16201 ggcggtatct ttgtaaaaaa agcagatccg ctcgtgctga aagacctcga tgagagagga
    16261 cttctgtttg atgcaccgaa gtttgagcac agctatccgc actgctggag atgcgacact
    16321 ccgctgatct actacgcaag agaatcctgg ttcatcaaga tgacagctgt caaagatgac
    16381 ctgatccgca ataacaacac catcaactgg attccggaaa gcatcggtaa aggacgtttt
    16441 ggcgactggc tggagaatgt tcaggactgg ggtatcagcc gtaaccgtta ctggggaact
    16501 ccgctgaaca tttgggagtg tgagtgcgga catcagcatt cgatcggaag ccgtcaggaa
    16561 ctgttcgaaa tgagtggaaa tgaaaaggca aaaaccgtag aactgcaccg tccgtacatc
    16621 gatgagatca cgatcaagtg tccgaagtgt ggcaagccga tgcaccgtgt accggaagtg
    16681 atcgactgct ggtttgactc aggagcaatg ccgttcgcac agcatcatta tccgtttgaa
    16741 aataaagacg tgtttgagaa acagttcccg gcacagttta tttccgaggc agttgaccag
    16801 acaagaggat ggttctattc tctgctggca gagtccacga tcctgttcaa caaagcaccg
    16861 tatcagaacg ttatcgttct tggacatgta caggatgaaa acggtcagaa gatgagtaaa
    16921 tccaaaggaa atgccgttga tccgtttgat gcacttcaga cttacggagc agatgcaatc
    16981 cgctggtatt tctacatcaa cagtgcacca tggctgccga accgtttcca cggcaaagcc
    17041 gttcaggaag gacagcgtaa attcatggga actctgtgga atacttatgc attcttcgtt
    17101 ctgtatgcga atattgatga atttgacgca acaaaatata cattagacta cgacaaactc
    17161 tccgtaatgg ataaatggct gctctccaag atgaacacca tgattcagac cgtggatgac
    17221 aacctggcaa attaccgtat tccggaaact gcaagagcac ttcaggaatt tgtggatgat
    17281 atgagtaact ggtatgtacg ccgcagccgt gaacgtttct gggcaaaagg catggagcag
    17341 gataagatca atgcttacat gacactttac accgctctgg taaatgtttc aaaagcagct
    17401 gcaccgatga ttccgtttat gacagaagac atctaccgca acctggtatg cagcattgac
    17461 aagactgcac cggaaagcgt acatctgtgt gatttcccga aggcagatat ggctcacgta
    17521 gacaaagaac tcgaagcaaa catggatgaa gtactgaaga tcgttgttat gggacgtgca
    17581 tgcagaaata ctgcaaacat caagaacaga cagccgatcg caaacatgtt tgtcaaagca
    17641 ccgaaagaac tgccagaata cttcgcagac attatccgtg acgagctgaa cagcaagaaa
    17701 gttaccttta cacaggatgt aaaagacttt acttcttaca gcttcaaacc gcagctgaaa
    17761 accgtaggac caaaatacgg caaactgctt ggcggtatca aacaggcact ttctgcactt
    17821 gatggcaatc aggcaatgga tgagctgaaa gacaaaggct ccctcacgct tgacatcaat
    17881 ggcagtgaag ttgtattata tgagaaagat ttactgattg atacagcaca gatcgaaggc
    17941 tatgtatctg agcaggataa cgatattacc gttgttctgg ataccaacct tactccggaa
    18001 ttgattgagg aaggatttgt gagagaactg atcagtaaag tccagaccat gcgtaaagag
    18061 gcaggttttg aggtaatgga cagaatccgc ctgtatgcag ctgacaacga aaagatcctc
    18121 accgtcctga aagaccatat ggatgagatt aaggatgaag tccttgcaga tgagattgtt
    18181 cttggaagta ttgccggata cgaaaaagaa tggagtatta acggtgaaag tgtacgcatg
    18241 ggtgttgaaa agacacagaa ataaggagga tttgctgtga gcaaaagatt tgacaaagaa
    18301 gtatttattg agagtgtgaa aagcaacctg aaaacactgt accgtcagac actggttgac
    18361 gcaacaaaac agcaggtatt tcaggcagta tcctatgcgg tcaaggatac gattattgac
    18421 aactggatgg aaacccagaa agtttacgaa aaagaagacc cgaaaatggt ttattatatg
    18481 tccatggagt tcctgatggg acgtgccctt ggaaataaca tgatcaacct gacagagtac
    18541 aaagaagtaa aggaagctct ggaagagctg ggctttgacc tgaatgtgat cgaagaccag
    18601 gagccggatg cagcacttgg aaacggcggt cttggccgtc tggctgcatg cttccttgat
    18661 tctctggcaa cacttggtta tgcagcatac ggctgcggta tccgttaccg ctatggtatg
    18721 ttcaaacaga agattgaaaa cggttatcag gtagaagtgc cggataactg gctgaaagac
    18781 ggtaatccat ttgagctgcg tcgtccggaa tatgcaaaag aagtaaaatt cggcggctat
    18841 atccgtgtag aatacgatga agcaacaaaa cgcaacaaat ttatccagga aggttatcag
    18901 tctgtacgtg caatcccgtt tgatattccg atcgttggtt acaataacaa tgtagtaaat
    18961 acactgcgtg tatgggatgc agaagcaatc aacgacttcc agctggattc tttcgataaa
    19021 ggtgattatc agaaagccgt agaacaggaa aacctggcaa gaaatatcgt agaagttctc
    19081 tacccgaacg ataaccacta cgcaggtaaa gaactgcgtc tgaaacagca gtatttcttc
    19141 atttctgcaa gtgttcaggc agctgttgca aaatataaaa agaatcattc tgacatccgc
    19201 aaattctatg agaaagcaac cttccagctg aacgatacac atccgactgt tgcagtagca
    19261 gagctgatgc gtatcctgct tgacgaagaa ggtctggaat gggatgaagc atgggaagtt
    19321 accacaaaga cctgtgcata caccaaccac accatcatgg cagaagcact ggaaaaatgg
    19381 ccgatcgagc tgttctcaag actgcttcct cgtatctacc agatcatcga ggagatcaac
    19441 cgcagatttg tggaagagat ccagaaaaaa tatccgggca accaagagaa aatccgcaag
    19501 atggctatca tttacgatgg acaggttaaa atggcacatc tggctatctg tgcaggttac
    19561 tctgtaaacg gtgttgctgc actgcataca gaaattctga aaaatcagga attaaaagac
    19621 ttctatgaga tgatgccgga gaaattcaac aacaagacaa acggtatcac acagagacgt
    19681 ttcctgcttc atgcaaaccc gctgctggca gactgggtaa ccgaacatat cggtgatgaa
    19741 tggatcacag atctggctca gatctccaaa ctgaaagttt atgtggatga tgaaaaagca
    19801 cagcaggagt tcatgaacat caaataccag aacaaactgc gtctggcaaa atacatcaga
    19861 gagcacaacg gtatcgatgt agatcctcgt tctatcttcg atgtacaggt taagagactt
    19921 catgagtaca aacgtcagct gctgaacatc ctgcacgtta tgtatctgta caataagatc
    19981 aaagatcatc cggaaatgcc gttctatcca agaaccttca tctttggtgc aaaagcagca
    20041 gccggatatc gtcgtgcaaa actgacgatc aaactgatca actctgttgc agatgtgatc
    20101 aacaacgata agagcatcaa cggaaaactg aaagttgtat ttatcgaaga ttaccgtgta
    20161 tccaatgcag aatggatctt tgcagcagca gatgtttccg agcagatctc cacagcaagt
    20221 aaagaagcat ccggaaccgg aaatatgaag ttcatgctga acggtgcacc gacactggga
    20281 accatggacg gagcaaacgt agagatcgtt caggaagttg gagaagaaaa tgcgtttatc
    20341 ttcggtctgt ccgctcagga agttatcaat tacgagaaca acggcggcta caatccgatg
    20401 gattatttca acaacgacca ggatatccgt cgtgtcctga tgcagctgat caatggagaa
    20461 tatgctccgg atgatccaga aagattccgt gatatctacg attctctgct gaacacaaac
    20521 agcagcgaca gagcagatac ttacttcatc ctggcagact tcaagtctta cgcagaagct
    20581 caggagagag tagaaaaagc ataccgtgac gaaacaggct gggcaagaat ggcaatgatg
    20641 aacaccgcat gcagcggcaa gttcacatca gaccgtacca tccagcagta tgtagatgaa
    20701 atctggcatc tggataaagt gaccgtagaa gcataaaaga atgataaaga aatagcaaaa
    20761 atgggcagtt ggatatgaaa aagccaactg cctgttttta tcttagtcgg agaagactcc
    20821 tataagtggc gagcaacaaa tttatcttag tgggagagca atctccgact aagataaacg
    20881 ctccgcgaga atgcacagcg attctgttgg gtaaatgtca gcaaagctga ccagaatcgc
    20941 gtgccaagtc tcacgtgcgt tcgacttgaa aaaaaagaaa gaacatcata gaaatatagt
    21001 ttctttcata ttcacttcgc ataaaaaacc caaaatcgga aaaaatgaga ataaggtaac
    21061 tgtttggttg caaatctgaa tttatgaggt gaagggatat gcagaagaag gatggaaaat
    21121 ggtttacgcg aaaaaaaggt gcgatgttgg ttctgacttt atgtcttgta ggggcggctg
    21181 cggcaacgac ctactatgct gttgatctgt cggatacggc acaggaaccg gcaaaagagc
    21241 atatggtgga tctgaatgaa agtcctgtcg aaacagaagc tgaggcggtg atttcatcag
    21301 aagcggaagc aaatctggct tctcaggcaa atgagatgga tgttgatccg gaatcactgg
    21361 caggcggcaa tcgtcagata agggcagaac aggaaacagc cgggcaggaa acggaagcag
    21421 agacagaagg catggagacc gaaaaagttt cctccggaca ggtcaacgca gtggtcagtc
    21481 cgacggtcag ctttgcgtcc acacaggaac ttcagtggcc ggtagccggc aaagtgctca
    21541 tggactacag catggattct accgtgtatt ttgcaacgct ggatcagtat aaatataatc
    21601 cggcactgat cttcggggcg gaagaaggag cacaggtcgt atctgcggca aaaggaatcg
    21661 ttgattccat cacaacaaat gatgaaacag gaacaacgat ccgcatgaat ctcggagatc
    21721 agtacacgct gatctacggc cagcttgaga atgtagccgt agcagaaggc gatgtagtcg
    21781 agagaggaca acttttagga tacgtggcac agccgaccaa atactactgc aaagaaggca
    21841 caaatctata ttttgccatg gaaaaagacg gagcagcaga agatccgttt ctgtatctgg
    21901 aataatatga tgccagggtc aaaagcctga aactacgatg tgcttgtaca aaagccgttt
    21961 tgacatcaaa aaaaaagatg attcacaaaa agtgagagaa agcgtatact atattaggct
    22021 atacggtatc cgtaaggagc tgcacgcagc atacaatatt tgcaatcgga ttcattttac
    22081 agattttatc ttagtccttt gagcttgatt atgaaataat gaaaatatac cgctttgccg
    22141 attgcttgaa ataatgcggg ggaaatcctc cgctttacat atacaagatg tgagatgact
    22201 cgcgtttctg atggaagaga gcaagcgatt tgtatcagag gcaggagcat gtcacaatac
    22261 gaagatatga aacagttgaa agtgaatgga aagtatgtgt tatacctata ttttaaagtg
    22321 cagtgacggc acgtattata ccggttggac aaatgatctg gacaagcgtc tcaaagccca
    22381 taacggcggg aaatccggtg caaaatatac gaggaatcgc agaccggtca cactggtcta
    22441 ttatgaggaa tttcagggaa aacaagaagc aatgaagcgg gaatatgcga tcaaacagtt
    22501 gacgagaaaa gaaaaagaaa acctgatcaa agaaaaaaat atgcagcaaa aaggttctca
    22561 ggatacaaaa agaggctctg agcaaaatca gaacctctga aattttatcc tttttcggca
    22621 agaatgccag aggcattttt gaataatcga aatgatttca cagtcattaa gcgatataaa
    22681 catacataag aataaagtgg catgcacttc ctgccatcac gaacagatgg aagatctcat
    22741 gtgagccgaa atttttgtgc tttgcatcga acagcggaac tttcaaagca taaatgatgc
    22801 cgccgacagt ataaatgata ccgcctgcca ggagccatcc aaaagctgcc ggagagagcg
    22861 aattgacaat ctgtgtgaaa gccagtacgc agacccagcc catgctgata taaagcacag
    22921 aagaaaacca tttcggacag gtgatccagc agatttttac gataatgccg atcagtgcga
    22981 ttgcccagac aagagcgaac aggaaatatc cgaccggacc tttcaaaacg atcaggcaga
    23041 ttggtgtgta gcttccggca atcagtacaa agatcatact gtggtctaac ttgcggagaa
    23101 tacgatttgt tctttctgaa agatttaaag tatggtagat cgtgcttgcc gcatagagca
    23161 ggatcatact cacaataaaa acagtcaggg aaatcacatg gatataatcc ggctcatgca
    23221 gagatttgat gatcagcggt gtggcggcaa acattgccat cagcatacca atgaagtgtg
    23281 tgatcgcact tccgggatct ttgatttttt ctaacatagt gaatacctcc tttgaaacat
    23341 gattcaagtc agatgacaga attgtgagag caattttcag atattcgtga tcatacctca
    23401 tgacttgaaa gcaatataaa atgaatattg aaataaaaac cgttatatgt agttttgaat
    23461 actatgtgta gttatatgta tattataccg aaaacataaa aaatgcaacc ctgtttttac
    23521 aatagctaat aatcagtccg ggctgtgcag aaatttacag aggataatgc gaagcattgt
    23581 ttgcaggaat tgcatgttgc tcaaatatgt gtctggtttc aaattaaaat gaacagttga
    23641 atttcaaaac gggatatgtt aaaatgactg gcatatgtga attgttactc ttccgcctca
    23701 cattagataa ataaagaggc ggtatttttg tgccttgttt ttcaaatata gttggggact
    23761 tttgcctccg acgtcattat tatgaacata taagtatttt gtagctgtaa gtgcatgaaa
    23821 cactgagcag tgaggagcgt tatgtcagtg agatttgaaa taaaagggta tttgcaacag
    23881 aaagaaagga tttaatatat ggaaatcata aaatttgata cacttggact tgacccgaag
    23941 attcttcgag ctattacaga gatgggattt gaagagccaa gcccgatcca ggcaaaagcg
    24001 attccagaag tgatggccgg acatgacgta atcggtcagg cacagacagg taccggaaaa
    24061 acagcagctt ttggtattcc gcttcttcag aagatcgatc cgaagaaccg gaaactgcag
    24121 gcaatcattc tgtgtccgac aagagaactg gcaatccagg tagcagatga gatccgcaga
    24181 ctgagcaagt ttatgcatgg catcaagatc cttccggtat acggcggaca ggatatcgga
    24241 aaacagatcc gttccttaaa aggtggtgtg cagcttgtga tcggaacacc gggccgagtg
    24301 atggatcatc ttcgccgtcg taccttacgt ttcgacaacc ttcacacgat cgtgctggat
    24361 gaagcagatg agatgctgaa catgggattc cgtgaggata ttgagacgat tttgaaagat
    24421 gcaccggaag agcgtcagac ggttctcttt tctgcgacca tgccgaagcc gatcatggac
    24481 atcacgaaaa attatcagaa agatgctgtt ctggttcgtg tcgtaaagaa agaactgacc
    24541 gtaaaaaata tcgaccagta ctattatgaa gtaaaacaga agaacaaaga agaagttctc
    24601 tcaagacttc tggatatgta caatccaaag ctctcccttg tattctgcaa tacaaaaaaa
    24661 cgtgtggatg agctgactga ggcactgaaa ggacggggat attttgcgga aggtctgcat
    24721 ggagatatga agcagagcca gagagaccgt gttatgaaag gcttccgcag cggcaaaaca
    24781 gagatcctga tcgccaccga tgtggcagcc cgtggtattg atgtagatga tgtagaggca
    24841 gtgtttaatt atgatgtgcc gcaggatgat gaattttatg tgcaccgtat cggccgtacc
    24901 ggtcgtgccg gtcgtgtcgg aaaggcattt acgtttgttg taggacgtga agtttataag
    24961 ctgaaagaca tccagcgtta ctgtaagaca aaaatctacg cacagccaat tccgtctctg
    25021 aatgatatta cagccatccg tctggaaaaa gtgctggatg agatcgacca tatcattaaa
    25081 aacgaagact taaaccgcat gacacgtatc atcgaacaga aacttaacga agaagactac
    25141 acagcgatgg acattgcggc agcatttctg aaacaggcac tgggtgaaaa tgaccagaat
    25201 aatgaccgtg ggcatatgga agatttcggt gataccgggg cagaagcagg catggtacgt
    25261 ctcttcatca acattggtaa aaaacagaaa gtacgcccgg gtgatattct cggggcagtt
    25321 gcaggagaat ccggtattcc gggaaaactg gtcggaacga tcgatatgta tgacaaatat
    25381 acattcgttg aagttccgcg tgaaaatgcg gctgatgtgc tccgtgccat gaaaaacacc
    25441 aaaattaaag gaaaaacaat taacgtagag cctgcaaacg gaagataaaa aaagcggaca
    25501 gatattgatt ttttcagagg atttcaatat aatagtgaga atgatatcag aagtgcagtg
    25561 gttctcgccg ttatagacgg cgggtcatcc agagacattt ttgaatctgg aatttaaatc
    25621 aaaaggggtt ttcaggaagg tatgacagat tttttagttc gcaggtttgt aagagacagt
    25681 gaaaacacag aatcaacgga ggtgcgtacc cggtatggga ttcttgccag tgtcgtcggt
    25741 attttctgta atgtatttct gtttgcggca aagttaatca tcggtacgct gatgcattct
    25801 ctggcagtca cggcagatgc gtttaataac ttatccgatg cggcatcatc gatcatcagt
    25861 tttatcgggg taaaaatggc agggaaaccg gcagatgcgg agcatccgtt cggtcatgga
    25921 agaattgaat acattgcagc actgatcgta tcgtttctgg tcattgaggt tggatttact
    25981 tttttcaaaa cgtcagcagg caagctgatg catccggaag atatcacgtt tgacttggtg
    26041 ccgtttctga tcctgatcct gtcgatcctg gtgaaattgt ggatggcagc attcaataga
    26101 aaacttggta aaaaaataga ttcaaaagtc atgctggcaa ctgcggcaga ttccctcgga
    26161 gatgtcatta cgacaagtgc gaccgttgtt tctattttga tctgttatgt tgcaaacgta
    26221 aatattgatg cgatcgcagg actgatcgta tcattgcttg tcatctggtc cggattttca
    26281 attgcaaaag atacactgga gccgctgatt ggacagaggg ttccggcaga attgtatcag
    26341 aaaatttcac agatggtaga atcctatgat ggtattgtcg gaacgcatga tctgatcgtg
    26401 cataattacg gaccaaatca gggaatggca accattcatg cagaggtgcc gaacgatgtg
    26461 aatattgaag tatcccatgc aatcattgac cgtattgagc gggaagtcgg aaaagagctg
    26521 aatatcactc tggtgatcca tatggatccg gtcgagctgc acgatgcgga agtacttcgg
    26581 ttaaaagaaa agacggaaca tatcatcaaa gcgttagaca gcaaactgag ttttcacgat
    26641 ttccgactgg tgaaaacaac gccggtcaat ctgatctttg atcttcttgt cccggcagaa
    26701 tatacacaga aagataccga ccgtgtattg catcagctga tgcagctgat ctcagaaatg
    26761 gaagctggtg caacctgtgt gatcaccgta gatcacagct ttgaagcacc ggaagaataa
    26821 ataaaataac aaataaaaag caataaaaaa gcgaatcaag gatacaatat ccgagattcg
    26881 cttttgtgtt atacatcaga tgtgaggtga ctaccgcctc tgcaggcggc gagaaacaag
    26941 aatgccagag gcattcttga accaaaaggt cttttgaccc gggcattttc tgtcttaaag
    27001 aatatcaatg tattctccgg caagtgcttt gttcatgctt cttacggcac agaatttacc
    27061 gcacatactg caggtatcgc tgtggtcatc ttccgggctt ctgctgtcgc ggatcgcttt
    27121 tgcagtttcc gggtcaaggg cacatgcata ctgtgcatcc cagtccagtt tctggcgggc
    27181 agcagccatg cggtcatcga tgtctcttgc tcccggaata cccttcgcaa tatccgctgc
    27241 gtgagctgcg atcttggaag cgatgatacc ttgcttggta tcctccacat tcggcagagc
    27301 cagatgttca gccggtgtaa catagcaaag gaaagcagca ccgttcatag cggcaactgc
    27361 acctccgatc gctgcagtga tatggtcata tcccggagca atgtcggtta caagaggacc
    27421 aagtacataa aaaggagcac ccatgcagat actctgctgt actttcatgt tggcggcaac
    27481 ctgatccagc ggcacatggc caggaccttc taccataacc tgtacattgt gatcccatgc
    27541 acgttttgtc agttctccga gacgaaccag ttcttcaatc tgacatacat cggttgcatc
    27601 ggcaaggcat ccaggacggc atgcatcgcc aagagaaatg gtcacgtcat attcttcaca
    27661 gatatccagg atttcatcat aatattcata gaatggattc tcttcgccgg tcatggacat
    27721 ccatgcaaat acgaggcttc ctccacggct tacgatattc attttacgtt tgtgcttttt
    27781 gatctgttcg atggttttgc gtgtaatacc gcagtgcagc gttacaaagt ctacaccgtc
    27841 ttctgcatgc atacggataa catcgataaa atcctttgca gtcagtgtag ccagatctct
    27901 ctggtaatgg atcacgctgt cataaacagg aaccgtaccg atcataaccg gacattcact
    27961 tgtcagtttc tgacggaatg gctgtgtgtt gccgtgactg gaaagatcca tgatcgcttc
    28021 cgcaccgagg tcaacggcac tcataacctt ctgcatttcg atatcataat ccttgcagtc
    28081 acgagatacg ccgaggttga cattgatctt ggtacgcagc atactgccga caccctctgc
    28141 acttaagcat ttatggtttt tgttggcaca gattgcgacc ttgccttcag cgacaagttt
    28201 cattaactta tcaacatcca tgttctcttt ttttgctaca gtttcgattt ccggtgttac
    28261 aattcccttg cgggctgcat ccatctgagt tgtgtaattt ctcatctctt ttttcctccg
    28321 ttggcattat ccaaatcagg taatgggtcg aaacgggaac acgtttcctc tcagccgact
    28381 tccgtcagct ccccctggtt tatgttggtt tcgcatatag attcatcctg cgtttatgcg
    28441 aatcgctaat aaattctaac acctgtgtat gatgtgtgca agtgtttctt gcaggatcag
    28501 gaagaatgtt ttgcaagata gcgatcataa gcattctgat atatttgaac agcttcatcg
    28561 atcttgtttt ttttcagggt gttcagatat tcttcgtaat tatcaaaaga tttgatcccg
    28621 gtaatgaaag cagttgtgta ttcatacata caggaattga cgttcgccat tatttgagac
    28681 agtctggtac gttcttcaga tgtaaaactc agactgaccg gaagcatata tgaagtatcc
    28741 gtggaagccc atacatcgca cataacagca ctggaaggag gcacacactg aagttcccgt
    28801 ttccagtcaa tataagcaga aggcaggccg ggagccagcg tatagttttt caatgcctga
    28861 taaaaagtca gtccatcaga attatgcaag atcagatcgg tgaaaacagg attctgatct
    28921 ttatcaaggg tataggtttt accttctgtt ccgtagcttg caaagcggga accttcatca
    28981 gaaaataaat agtcaatcca cttcagaaca atttcaggat gttcgcattc ggaagagatg
    29041 taaaacatat tatcactgat gctgtatttt ttctgtatgt gcagcgtatc atgttcgttt
    29101 aatttgggcg gagaaacagc aataacctta atattctgat cttttgcaga aagatcagaa
    29161 ggcatggtat acataccaaa ccatgcaccg gaagtgccgc tggcgatcat tttactgtct
    29221 gcataagtcg agttgcctgt catataatca acatctatca gacctttctg ataccaacgg
    29281 ttcattaaag tgagataatc cttccatccc tgcctggcag gtccataaaa gaccttgccg
    29341 tccatttgaa aaaaatcata actgactcca aaaccggcat tcagactttc atcctgtgca
    29401 taagcattgt atgaaatcca caatggggct gtggctcctt tcctttcttt aaatgcagtg
    29461 agaaccgttt cccagtcatc ataagtgaca ggaatctcaa gccccagttc atccagccag
    29521 tcttttcgaa tctgaagacc acaccatggt ttttgcgagt tttgttttat agaataaaca
    29581 gctgcaattc gaccggaatc cgtgtaggcg atatctttta tgtccttttg ctgaatgatc
    29641 ttatagtaat tcggtgcata ttttggaacc agttccgtca gatcacagat gtccccctga
    29701 gcgatcgcat catccgcaga aatattccca tagatggatg aaaaatttcc ggcaccgatc
    29761 aaatccggta cggaatcgct gttttgctca aaacggtttt gaatctgcag attggataaa
    29821 gaagtgatcc ag
//
LOCUS       DAWDWH010000005        28975 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_960_length_28975_cov_15.386999, whole genome shotgun sequence.
ACCESSION   DAWDWH010000005 DAWDWH010000000
VERSION     DAWDWH010000005.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 28975)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 28975)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..28975
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_960_length_28975_cov_15.386999"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8961..8970
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         9525 a         5057 c         6939 g         7444 t
ORIGIN      
        1 atcgaaatat cttgaagagg gtaaagatat gctggaatta acgatttatg gaattggatt
       61 tttcttatgc acattgctga ttcaatatat gctttttaaa ttcaggaaaa atcataatca
      121 cggagccaat ggtgtgtggg ttggaaatag agcaattatg gtgctgtgtg gtcggcatta
      181 ttaattataa ggttgaatat cttgcggcaa tactgggatt tatctgtgcg gatgaggctg
      241 gtaagacggc tggatggcat taaggatgaa gggagatttg atatatgaaa tactgcgttt
      301 ttggaaaagg aaaaggtgct agtaaaatgg ctgatcaggt cgtaaccaaa atgttgcagg
      361 agtattcgcg tcaattgttt ggtttcaccc gtgcggagtt tgatagtatc gtggaatatg
      421 atgtgataaa catcatggca tattattatt ctaagatgaa tgcacagcag aaggctgtga
      481 ttgtaaatgt gctgcaggag cagtttgaac tttcttacat ggatgatact ttagaaaagc
      541 ttgctatgtt tgcagatgat gaagtatatg ttcctctttc ctgtaagttg atggatagac
      601 tcggagatag ccgtgtcaca ataatcgtgt atgaaaccat ttttatgtgc tgtgcaaata
      661 tgaccaagaa ctattggaat cagagtaatt cgtattctaa ttacttggtt ctccttaata
      721 cagcattttt tcatgcactt tcttataaat tgcatatcaa ggacagctat gtcaactcca
      781 tgaaggctct gtatgaccaa gatggatacg atgaattcgt tgaatctctg tatgaaaagc
      841 atttggatga tatggactgg gacgctgatg ttgaattaga tgttttggat gagctgatcg
      901 atgttagcaa aaccaataca ttttttgatg aaggaacaca gaaagagtac atggacgcag
      961 tggaagctat gggtaatatc cgcagagaac atgtaaatac tgtgcgtgaa gaagacgaaa
     1021 caaagggatt tgctcgtatt gtagagaaaa caggtatttg tgagctccag agcgtagata
     1081 ttcatggttt cgaatgccat ttctgtgaag ctaaatacag tgatgagaag tacatcatca
     1141 tttaccagaa agatactcat attcgtgaca ttattgataa tgaagaggaa tggagcaatt
     1201 tccagagaga attgtaccgt gaagatatcc gccaggataa cagcaaggct atggtatata
     1261 tcgtatacat tctggatgat aattctgaca atattccgat tcaggttatt gagagtaata
     1321 agacttacgg cagaaaatat gtattttctg aggatgagac gatcaccttt atcaatggta
     1381 ttgtaaaaac ttccagtgat gagattagtg cagcttctcc tgttcaggag tgggatcgta
     1441 ttttgagaga agaacatctg actgcatgtc tgactgaacc atattccgca aagaaggtag
     1501 cgagttatct gggtggacag cgctttgatg cggattatgt tcatgacgat gattattcta
     1561 cgatgaagca ttctaaagta ccacagatta agtgggtgaa atcattagag actactgggt
     1621 tcagagattt ctgttttgac cagaaaatga tgacctttgg tcagattaac cttttttatg
     1681 gagctaatgg atctggaaaa acatccgttc ttgaagcgat tgagtatgca cttacagcgg
     1741 aagtccgtcg agtaaaggac ttcaaggtaa agcttcctac tgatagtttt cctaaattac
     1801 atatatacga tacagaagcg ggagttcata catttacacc tggattctct aaaaaaaata
     1861 ataaagaaat tgagcgtgta tggtatggtg ttccgattgg gagaactaaa tccaatctga
     1921 atgaaaactt taaccgtttc aatgcatttg actcagaagc agcttataag tttattcatg
     1981 agtctgataa tagtgaagat agttttgcat ctatgttcgg aaatctcatg ttcggcgaaa
     2041 cagtcgtcga tcatgagaag aagtggcaga gatttaagag agcctttaat gatcgctaca
     2101 cagaactgcg gagcgaactg agtgaagcca gaaatatggc ggatatttat gaaagaagct
     2161 tggctcaaaa aacagatagt tctaaatctg aggagattga ggctggtatt attgcactaa
     2221 aactcatagg gcgtgctcgt cttccgaagg gatctagcga cagatatccg aagattttag
     2281 aagaaatgaa ggcaattcgc aagtatgtgg atgttctttc tgtgcacaat atggaaagaa
     2341 tgagatttaa ggcgattgca gcacaggtga ctgaggcaaa gaagaataat ctcctttata
     2401 ccagacggcg aaaagataaa tcagaggaaa ttaccagact tgctgaagaa aatggaacta
     2461 tcaagaagaa aatatttgat gaaagagaaa aacagaccgg tattcagcag agactggatt
     2521 gtgttaacac agacattcgc aattggacaa ttattcagaa tgtacttagt catgaagaga
     2581 ccattaaact cgtaaatgat cttctgggtg aactgaccca gattgagcgt gaactgtatc
     2641 atatttcaaa aattgaacaa agacctgcta ttattaattt cctgaaattg aatgatcacc
     2701 agggattatc cgaggatgaa aagcagcact atgaggcgga gctagaagcg gccaaagcaa
     2761 gaagacgtca ggttgaaaac aaatataacg aagagaaaaa ggctttcggg gaaaaagaac
     2821 aacaagcgat tgaacttaga aaaatcggca aaactctcat gagcgatgca aagtgtcctc
     2881 tttgcggtca tgagtatgat actactcagc agttgattga tatcattgac aatgcggtag
     2941 tgattgatga caaaatggat gctttgatta ctgaaattca ggaactcagc agacgcatta
     3001 tggagctgga gaagattttg gatcgtcaaa agctgatcga tagagcaaag aaggaactgt
     3061 tgaaactggc agacgttgtt ccaatagtaa gaaaatgtgg ctcagattat aaaaagttgt
     3121 atgaatatgt tacttcaaag gctgaaaagg agaaaagaaa agattgttga gcagcaaaca
     3181 gctcttgata accagggatt ctctatccgc aatatcaatg catgtaagga atataccagc
     3241 acggattcta catatttaga gtataagaaa aatggaaagg gtatctacgc agaattcctg
     3301 cagaatagat tgcagaaaat tcagcaggaa ctggcattat ctgaaagtgc tatcgtagat
     3361 caccagaaaa agattcagca gaatgagcag agggaagaac ttctaagaag tgagatccat
     3421 agtctggaat cacagctgga agctcttgat atggatatta accgtgccat cgagcaggca
     3481 cttgaaacct tgaggacaaa atttgaattg ccggctacag gtgtactcgg cgaatgggta
     3541 ttaaagtatc atgcggtata tgataagtgt gaattagaag tagaacgtct ggaagctcag
     3601 aacttaattg ctttggaacg ccagatgtta gctgaataca aggcaacaat caagagagtt
     3661 gagcctatgg ttgagcactg tgcgagagca gtacaggctt ttgaaaagat gccttccctc
     3721 agttctttcg tagaaaaggg aattcgcagt aatattcagc agatcagcaa attcttcaag
     3781 tggatgcatc attcaggaga atttgaaaag ctggatatcg acgataaggg tatctatgcg
     3841 gttcgaggcc tgaataatca ggaagtaaga acttatgaga tgagtactgg tcagagatcc
     3901 accatcgcta tggcagtaat gtttgcatta catatggcag caccggatgc accacaattt
     3961 ttgcttttgg atgagccgct tgccactatg gatgatacac aggtattgaa cgtgttggat
     4021 attctgaaat ctatggcaga gcagaatact cagatattct ttactactgc aaacggtatc
     4081 atgattaatc ttttcaaaga atgttttaaa aatacaaggt tcgactataa agaataccag
     4141 tttgtaaaga gagtaaaccg tccttcagaa atcaaagaga gcagtatcaa cgatacgaaa
     4201 tcaattgagg aacttacact ggatgatctt acccttgatt ttcatcagtt tgcacagatt
     4261 cgtaatattc ttcgtaagaa tcaagagaag ctggttgccc gagaggaatg ggaagaactg
     4321 ccggaagaag ttgaaacggc ggctgcagca ggtgtacagg tacagcagcc gataattcag
     4381 acagcaccgg aaaatttcta tatgacatta gatagtgacg aaagaagagt tctggacgtt
     4441 ttagtagcag atcagccgga aagtgttact gagtttgtga agcttgtttc accgttcccg
     4501 aattacaaaa cgattctgga gcgtattaat gaaaaagcac ttgattttta tgaagaaatc
     4561 attatcaata cggatgaaat gctgccttat gttgaggaag attatctcga tgaactgaaa
     4621 gagcagcatg atgtttacta tgacaagaac aaagctgatc tgatgattga agaacagcgt
     4681 cgtcttgctg aggaaagaag tgctcgactg gtcgatgagt tgaaaaaatc cgaagcagag
     4741 cgtcgcaaac tcgaagcaga aagggaaaaa gaggcagagc ttagaagaac tgaagcagaa
     4801 cgcaggactc agttggaaga agaactgtgc aaggcagaag aaaaatctcg tcagcaggaa
     4861 gagcagcgta aagcagatga tgcccgcagg caagaagaac tgcgtaaggc agaagaagct
     4921 cgtcagcttg aagagcaaag aagagcagag gaagagaagg ttttgcagga ggttgagcaa
     4981 caaaaaatac gatacaaaca gatgaatgtc tgccagtatt gtggtggtgt atttaccgga
     5041 ttatttaata agaaatgtaa ggtttgcggt aagccaaaag actatgcgta aatcggtagg
     5101 ggtgtttgga tatggaaaaa cgtataccta aaaaaacata tagaaacatt atgaactctc
     5161 tcgggggcgg tactgttccc cgagaaggtt tggggtatat tgcagttgga agagagaaag
     5221 aaatagactc tcttcttcgt gatacagaga ttatccagga gggtggagct acattccgtt
     5281 ttgtggtagg tgattatggt agtggaaaaa catttctgct tcagacaatc aaagagtact
     5341 gtgtaaagaa taattttatt gtggcagagg cggatttgtc accagataga gccttgattg
     5401 gaaattctgc aaagaaaaaa ggattggcaa tataccgtga actgatggcg aacgtatcca
     5461 ataagacaaa tcagtccgga agagcgttag ccaaagtact ggaatcctgg gttaataata
     5521 tgtttgcccg tgctgccatt cagatgtctc agaatccgtt tggagagaca aatattgaac
     5581 gaattgcaga aacgctcatg ctagaagatt gtgcaaaact gcagtctctt gcatatggct
     5641 atgaatttac tatgggattg cacttattct ggaaggccag tagaacttct gatatgagtg
     5701 aaaaaatgac cggacaggaa aatgttctcc gatggttccg tggcgaattc aaaaccaatc
     5761 aggaagcaaa aaaggaactg ggcatcaatg caacagtgca ggatgaaaac tggtttgatt
     5821 atatcattct gtgggcacag ttttttgtcc ttgcgggata taagggattt attgttctca
     5881 tcgatgagct tgcttatatt tgtaatacag caaatggtat cactcgccag aataactatg
     5941 aaaaaatctt gatgatgtat aatgcagcac ttcagggcaa ggcagaaaac ctgggaatta
     6001 tcatgagtgg tataccaaag agcatctatg ataagaaaaa aggtattttc agttatgaag
     6061 caatgcgttc gcgtttatct actggttcat accaggatcc aagcatcgtg aatatgatga
     6121 ccccgattat aaagatactt ccgctgacga gagaagaaat gtatgtactc ttagagaaac
     6181 ttgctgaaat acatgctcag ttgaatgagt atgaaaaagt cattacggag gacgagatga
     6241 tcgattttgt aaagcttgca tatttgaaaa gggagactac ttttattacg ccacgaacaa
     6301 tgatccgtga ttttattcag attttggatg ttaaacgtca gaatcctgat aaaggaatca
     6361 gggatattct tgctgcttac agatttgcag ttgatgaaga acagaattat gaggaaattg
     6421 acgattaggg aaataattag cccaataaag attagagaac ttactccaga gcggattcta
     6481 tcagaaaaag ggattctctt caggacaaac cgaagtatac aggcggaagg ctcctttgga
     6541 gattgaaagc aggatatgca gttcctcaga tatttaagca aaggaaatgc aaatgttctt
     6601 gcagagagta cgttgcttgc catggcaaga aacataaaca aactacataa taagattcaa
     6661 aatgcaagaa ccggaacaca tttatttccg ataaaaagtg cctgaatttt gacttatcaa
     6721 aataattttt caagccatca aaaatggctt attaaagtgc gtctttaata atagcctgta
     6781 acaatgaaaa gcgttttttg atggatttta tccttaaaac agcaaaaagg aagctgccgc
     6841 ttcacgaaaa ttcattcgtt aaagcgacag ctccatctct ttcttttcat atgtatcaga
     6901 aactgatata ctggaaacat aatggtaaat cataacagga aggagcagga gcagtgtcag
     6961 agaaaaagaa gtttacatta tatgccggaa gtgtggcact gtgcgtgtgc actgcagttc
     7021 tgcttcatta tgtgtcggtt gcagatcctt atctggaatc cgcctgccgt cttttaagac
     7081 cttttattta tataggactt tatgtggtgt gggcgatctc tttccggaaa aggattatcc
     7141 agaaagaaat ccgtcgttgc ctgactgcaa tcgcagcaat gatgattttc tggatgttta
     7201 tccggatgtg caaatttgaa atatcggacg aaatgccgac agcatggcga tacgcctggt
     7261 atttttacta tatacctatg ctgctgatcc ctacggtcag tctttatctg gccttttata
     7321 tacggcagcc ggagggctat aaattaccaa aaagacgatg gctgctgttt cttcctgcac
     7381 tttttctgat cggaattgta ttaacaaatg atatgcacca gttggttttt acctttccga
     7441 agggaaaact tggagaagtg tcttcttata aaacaggagt ttatggatat ggaagagggt
     7501 attatattgt aatcgcatgg gagcttggat gtgcactggc ggcacttttg atcattctgt
     7561 taagatgcag gaaaattaaa aataaaaaaa tgggctggtt gccttttgcc gcttatggag
     7621 tggcggcggc ttacagcatt gcttattatc ttgatctacc tttctggaaa atagtttcaa
     7681 aagatatgac agccacattg tgccttctgt ttgctttgat cattgaaagc tgcatccagt
     7741 ccggactgat cccctccaat acaggatatg cacagttatt tgcagcatct accatttcgg
     7801 cacagatcac agaccagaat ggaaaatgca tttatcagtc tcagggttct gaatatattg
     7861 caccaaagat cgcaaaacag gtaatccaaa gacctgtcat gtcggaaaat ggaattcggc
     7921 tgtcagggaa accaatctgg ggaggctatg tcttatggaa agaaaatctc tcagaactac
     7981 aggaaatttt aaaagagctg gaagaccgta aagaagaatt aaaagatgca aatctgattg
     8041 aagaagaaaa tctgagaaca aaaaaacaag tagaaaaact ttccgtccag aatcagctgt
     8101 atgataaaat acagaaacaa acagcaagac agagtacgct tttggcgaaa ttcatggaag
     8161 catatgccat ggaagaaaat gagaaggaaa gaaaaaaaat attagggaaa attgttgtga
     8221 tcggagccta tataaaaaga cgcagcaatc ttatccttat cgcagagcag agtgcaatgt
     8281 ttccgataag agaactggaa ttatgcttca gggaaacgat aaggagtctg gaatggaatc
     8341 atgtggaagc agcttttgtc acttctctgg atgaaatccg aagtgaggat gccatgcaga
     8401 tttatgattt tcttgaggca gtcattgaag aatcactcga agatctgtcg gcattcaccc
     8461 tgaatctcaa acgcagggat gaagagatcc ttatgtcttt gagtgtggaa tgtaaaacaa
     8521 atctgcaaaa agttgccgga cgataccagg cttatgcaga gcaggatttt gacggtgcat
     8581 ggctgctttc cctgctactg aaaggggggc atgatgaatg atgaaagcat ttggaagctg
     8641 tccgccggaa tatcggatca tggcagtctg gattttcatt tttctgacat tgctgtatat
     8701 gattctactc atctgtgagg gaacagggga aagaaaaaaa aaggcgatgc tgcaggacag
     8761 cctgttgctt atagctgctt tcttgataac gtccgggtta tgtgtgcggc agagatttta
     8821 tggagaagag gatttcatgg tattcttaaa aatgccgtat ggaatcctga tcattatggg
     8881 gatctgcgga tttttatttg cgggaataca ggcatttgga atttataaac gtcgcacaaa
     8941 ctgtctgagg gaaaatgcga nnnnnnnnnn tcatgtattt tatgattttg atctgaaagt
     9001 atactgcttt tcagacggaa gcgtatggaa attttcagaa aaagagatca ttacggcagg
     9061 tggagatcga ttttctcagt tccttgcttc agaagtatca gaattatgtc agagcaaggc
     9121 attgttaaaa aaagaaaatc agaagcttca ggaaatgtcg gcggctatga aagcactttc
     9181 caaaaatgtc gtaactctga ccagagaaga agagacatta tctatgaaaa tgagagtgca
     9241 tgataatctg ggatacagcg tgctggcaac acagcgaatg ttgatgcggg aatccgaaga
     9301 agatcgtgat atgtttctct cgcaatggaa gcagacatta gacctgctga ataaggataa
     9361 tgagtctgtt gaaggcgaac agctgcacag gcaggtgcag gaaagggcga aagttctggg
     9421 agtaaaaatc atttatacag gagaaaaagt ctgggaatct catatatctg aactgatgga
     9481 tatcatactt ttagaggcac tttccaattg tgtccgccat gcaggagcaa gcgaactgta
     9541 tgtgaaattc agtattgaag agcaggagtg ggtcatggtg ctcacggata atggacagaa
     9601 accggaaaaa aacatcaaag aaggcggagg gctatccggt atacgaaaaa gggtggagca
     9661 atgtggaggc agcatgagga tcactgcagc cccacggttt tcgataaccg ttaagctttt
     9721 aagggaggtg cggagatgat acgggtactt atcgtggaag atcagcgtat ggcaagggaa
     9781 gatatggaaa attatattca gtccagtggg agatactctc tagaagcttc catcacaaat
     9841 gcagctatgg cagaaactgt gtgcaaacag agcaggtgtg aacttgtcct gatggatgtc
     9901 tgtacagagg atgatgagag cggtcttgtg gcagcagagc agattaaaaa aacgatgcct
     9961 cagataaaga taattatcgt gacttcttta gcagagtgca gttttattga gagggcacat
    10021 aaagcaggag tagaaagttt ttggtataaa gatgccggaa aagaagagct gctggaggtt
    10081 atggaccgca cgatgaaagg agagagtgtc tatccggacg ctccgccgac ggtgatgatc
    10141 ggaacggcga aaagttgtga ttttacacag ggcgaactgg ctgtcctgcg attagtggtg
    10201 gaaggagaat cctacaaaaa aatcgcagag agtttgtgca tatcaccgga gacagtaaaa
    10261 tggcatatta aaaatatgct gcagaaaaca aattttgatt ccaaaacaaa actggcggta
    10321 gctgttacaa agaaaaatct gattataaac ggattttaaa aaatgaaaaa tcccattgct
    10381 ttcctgatag tgaggcaatg ggattttttt tgcaaaagta cattttacta cccatctggg
    10441 tagtgcttaa gtctcaaaaa acagatatca tagtagtatg taaaagtgaa acgagtgtaa
    10501 aaaacaggga gggaacagag tgtgagaaag gtcgttatcg atatgcagaa ttatttattt
    10561 tcggatgcca tagcaaaggt gctcaaatcc tatgattctg atttttttat tgagatgtca
    10621 gacacaccgg ataaaacagc agaattatgc agagtcattc agccgcatat tctgatgatg
    10681 gaaattacag gtcaagcatt gtggtcatta aaagaacgtc tggcaatctg ccgggaagtg
    10741 cgtaggtatg ctccaaactg taaatttatt tttacagtag atgagaaagc aaatgcagag
    10801 accgcaaaaa aagtagtaga atgtaaaaaa acgggtctga tcgactgttt catttacaat
    10861 accatttcat ccagctatat gactgcagtt ctggacagtc tgtaagctgc agtcccggaa
    10921 ggagaaaaga taaacatgag atctgttatg aaaaggatct gcctgtctct ggcagtgatc
    10981 gcagtcctga tctttacagg gtgcgggcag aaaaaaacga aagatccgat cactgtcaca
    11041 ttgtggcatg tatatggcgg acagacagaa tcacccttaa atgatctgat tgatcagttt
    11101 aacgaaacga taggaaaaga ggaaaatata tgggtacagg ttggcagtgt tacgaacacg
    11161 aatacaatac atgaaaatgt actggcatct gcatttggag atccgggagc ttccgaactg
    11221 ccggatatgt ttgtttccta tccgaaaact gttttggcta tgccggatga caccgttctt
    11281 gtagattact gcgattattt tacgaaagaa gaactggacg agtttattcc tgcttttctg
    11341 gaagaaggtg tcattcacga cagactttct attttgccgg tagcaaaatc aacggaagtt
    11401 atgtttgtca ataaaacggc atttgaccgt tttgcaaaag aaacaggggc ggatatcgaa
    11461 gatttaagaa catgggaagg gctctttgcg atgtcagaac aatatgctga atggacagat
    11521 aacaagacac caaagatccc gcatgacgga aagaattttt ttgttcatga ttatcatttt
    11581 aactattttc aggtaggcgt agagtcattg ggcgaaaatt tctttaaagg agaaaatctg
    11641 gcttttggac cggaatttga aacggcatgg gagccttatg caaaagcggc actgtccgga
    11701 ggtgtatggc ttcgcagcgg ttatgcgaca gaacctttac gcacgggaga ttccatcgtg
    11761 tcagtggctt cctctgccag tgttctgtac tattctgacg aagttaccta tgaagataac
    11821 acatcagaaa aaatagagat tgtcgccatg ccatgtcctg tattttccac aggggaaaag
    11881 atggtcatgc agcggggtgc cggtatctgt actgtaaaat ccacaccgga caaagaaaaa
    11941 gcctgcatca cctttcttaa atggctgaca gaaacccgga aaaatgtgga atttgtaacc
    12001 agccttggat atatgccggt aaagcaggag gcttttgatg cctgccttcc ggaagcgatc
    12061 aaggagctga gtgatccgat gtatgtttcg ctttatcagg cttttctgga tacgcaggag
    12121 aattatacat tttatacggc tccgcagctg gattcctatc tggatctgga aacaaggttt
    12181 gaagaacttg tcaggaaagt attgtcagta aagcgtcagc agtgcataaa caaaccggaa
    12241 aatatagata aacttgtcaa agagacatta gcggatttta aagtggcata cacacaataa
    12301 tgtctgaccg gatgaatcag aagaggcgac gggcgtatga ggaaaaagag aaaaaattta
    12361 tggaaaatca taaaaggaat aaggaaacaa agcatctgta tgcagcaaag actgattgtt
    12421 tactggtgtg tagtgattct gacccttttt tcggcagcag ttctgttgat gagtattttg
    12481 ggagtcattc cgggaatgga tttgaaagta ggggaaatgt tgtctgccca gcagaaaaat
    12541 acggtgtctg ccatgacaga gcagaccgac attatgatgg cgagaagcat ttctctatca
    12601 gaagaaatca caaaagagtt aagtcaatac ttgacagcca atggaaaagc attctccgat
    12661 ttaaatgaca atcctcagtt aattatggat ttggaggcag ctttgtattc ttcactgaga
    12721 tcagcactgg atgtgaagta ttgcagcgga gtttttgtta tattaaatgc aacggtaaat
    12781 acggaaacgg agtacgcaga cacatcacgc atgggaatct acctgcggct gtctgattta
    12841 aaggcagtaa atacaagcaa acagcacgtg gtttttttca ggggaaatgc ttatattgca
    12901 agggcagagc aggtacagct ccataaccgt tggaatttag agtttgatac atctgcattg
    12961 tcaggctatg aacagatcat gaaatttaaa ggaaaccggc tggcagagag ctgtctgtgg
    13021 accgatcggc tcaaactgaa ggatacatgg gaagatgtac agcttttata tgtgccggtt
    13081 ttagacagca ccggaaaagt gcgggggctg tgtggtatgg agatgagcaa tctatatttc
    13141 agactttcct atcctatggt agaaggatcc tatggaaata tggtgacagt agtggctcca
    13201 atggacgaaa aagggaaaat ttatctggat aaagcaatgt tcggggacac aaaagacacc
    13261 catctttcac caaccggaac gatgaccgtg aagaatggaa agcattataa cacttattcc
    13321 gctgctttca gaaagtatat aggcaggcat gaactgctta acctcaaaag ctgcaatgga
    13381 atgccgctgg cggtactgac attgatgtcc gaagcgaatt atgaaaaatt aacagcagaa
    13441 aacagaagag cctggattac cggtacgttg atctttcttt ttctcctgtt gcttctttcc
    13501 atcactatgt ccaaccgttt tgtaaaaccg attttgcgga gtttaaaagc actgcaggat
    13561 gatacgctga cagacgggaa tctgtcggga atttcagaag tggatgcact ggttgatttt
    13621 atgcagacaa aaagaaatac agaaaaactg gaaaatggtg gaattcctcc gaatatagaa
    13681 gaaatgcttg aagcatttgc cttgcgtgtg gaaacgctga ctccggcaga aagaacagtt
    13741 cttcagtatt atattgaagg atatacgatc caggaaatag cttcgctgct ttatatcagt
    13801 attgggacgg cgagaaagca taatacaaac atgaaccgaa aactgggaat aacagccagg
    13861 gaagaattaa tgctttacat agacctgttt cgtaaatgca atcagctgga taagctgaca
    13921 tacacacgga cagagtgaaa aaagagtcgt acagactctt tttttattcc cggataaaaa
    13981 atgagaaata tctatatata gttttcgcaa aacaaaaaaa ccaacatata gtatactaat
    14041 cattcgtatt ttggaaaatc aggctaaaaa tacgaatggc gaccatatca tttattttct
    14101 ggatctgtta tgctttcatc acaggaaaac ggaaagcaaa accagacgaa accagaaaag
    14161 caaacaaaac aaaaaaagga aggagaaatt tactatgaaa aagtatgaaa tcttaggagg
    14221 aaatttaccg gttgttgtat gtgaattatc agcaggagaa tccatgatca cagaaagcgg
    14281 aagcatgagc tggatgagtc cgaatatgaa aatggagaca atctccggag gcggaatgaa
    14341 aaaaatgttc ggcaggctga tgtcaggtga ttctatgttt caaaacagat ataccgcaga
    14401 aggtgcagac ggaacgattg catttgcatc aagttttccg ggagcgatca aagcactgga
    14461 tatcagagac ggtcattcta tgatcgtgca gaagagtgca ttccttgctt cagaagaagg
    14521 agtggaactg tccatgcatt tccagaaaaa attaggaaaa ggtatgtttg gcggggaagg
    14581 tttcattatg cagaaactga gtggaaatgg aactgcattt gtagaaattg acgggcatgc
    14641 cgtggagtat gagttggatg ccggacagga aatcctgatc agtacagggt atttggcagc
    14701 aatggaagaa acctgcacaa tggatgttgt agcagtaaaa ggagtgaaaa atatgctggt
    14761 cggcggagag ggcgttttta atactgttgt cagaggaccg ggtaaagtga tcttacagac
    14821 aatgccgatc agtaaggtgg cagaattact gactccgttt tttacaaaaa ataagtaagg
    14881 gaaggagcga gagttatgaa gaaaaaatgg aatttattat ttttgtttgc atttattgtc
    14941 agtatgataa tactggccgg ctgtggaaaa acagcaggag aggcaagtcc gtatgaagga
    15001 aaatggatag cagtgtccgc tcagatgata ggaatgtctg tcagtgtgga tgaaaccttt
    15061 gggggagctt tcgaatttga ggtaaaaaat ggcggaaaag tttctttttc agtaggagaa
    15121 gttaccggaa atggaaaatg gtctgcaaaa gatgacaagt ttagcttaac tatcgaagga
    15181 gaagagatgg tcggaaccat cggagaaaac aacatttctt ttgacgatat gctcggaatg
    15241 ggagtaaaag tgattttcgc aaaggaagga acggatgcca tggatccggc tcgctatctg
    15301 acaaaggaag agaaagtggt cataggtgag tgggctgctg aaagtgtgga agaactttta
    15361 ggagacggac cgcagacatc tatggaaggc gtagagaata tcagtgatgc actgcgtctt
    15421 aacttcaaag atgaccgtaa tgtagcagtt gtttataaag gcgaggagat tgggacattc
    15481 ccgtggtcag ttgcaatggg ctactgtatg atcgaatcag aaaatccgtc tctttccgta
    15541 acgatcaatg acgatgatac tttgaaagta gattacagcg atgacgaaga ttactataca
    15601 tttcattgtg tgaaaagtga cagtaaataa atgaaaaaga cataaatggt gaaaggaggg
    15661 aagagcccat gaaaaagaaa atgtggtttg tgctgtgtat catgacaagt gtcctgctga
    15721 caactgcctg tggagagaaa aaagaacctg gagaagaaaa aaaagaaaca gctgtgcaga
    15781 cagaaacagc tatgcagaca gaaacagctg tgcagacaga aacagctatg cagacagaaa
    15841 cagccgtgca gacagaaaca gaatctgatc aggacttcca gggcagggaa agtctgaaac
    15901 ttggtttatt tactgtgtat tatccggaag aatggaagta cgacaaagaa agaatgcagg
    15961 aagatgatga ttactcttac gtcagatttt ttgacggaga aacagcagat gattccggga
    16021 acagcattta tatagaagca acaaaagaag atgcatataa ataccggaag ggtctgattg
    16081 ccttcggtgt ggatttaaag gaatatgcgg aaggaaaagc agagactgta tcaataggga
    16141 atgcagaata tgcggtaatg ccggaaaatg attccggaag gtgcacgtat atgtatcgcc
    16201 atgagcagtc aggtacgtct tatgatatca gagtagaagg agaggcagca agtgattctg
    16261 tcaaagaatt gctggaaggc attgtgatgg aattaaaaga tgaaggaaat gaagacgcac
    16321 cgtggccatg ggagggagag cctgttgagc ctgtattgac agaacagatg gtaggttttt
    16381 atacgatcgt tccggaatat ataccattta aagaagcaca gggtgtcatg cagattatgg
    16441 atcaccagtt tgtaaaacat gacgatcagg tataccattt gctggagaat aagctggata
    16501 cttacaagta ttcagaaagt ggactcgaat ttgtttcctc aatggaactg gaaagagatt
    16561 gtgaatatct ctccggggac aacagcggaa tgctgtatct ttcaccggga atccaggatg
    16621 tgattggtgt aaaagacggc aaaaaggtgc ttcagacaac gatagacggc gatttgaata
    16681 tgcatccgtc cggagaatgg ggaatcagtt tctgggtgaa cagcgatacg cagaagattg
    16741 cgaatcaggg cggtaacctg acagctgaac cgtggatact gaccggatta aataaagatg
    16801 atgaacgcaa gggaccattt tctatgatag atgacgtcca gatcacaaat gaccatatta
    16861 tggttgccgg ttccatggct gcggaagacg gaggcacaaa aattattgtc tatgattatg
    16921 aaggcaatca gcttttggaa ttaggcggag cagacattgg ttctccagac aggcttggtt
    16981 ccatcacggg catggcagaa acagaaaatg gttttgtagc ggcagacgga aatatgcgga
    17041 aaatacaatt ctgggcaaaa gacggaaccc atgtgggtgc catttctaca gatgatatat
    17101 ttggcgtcag ttatccatgg ctggaagata tgcagctttt agatgatggc tctcttttga
    17161 tcctgctgac acagcagcga gaggacggat cagcaaatga actgatgttt ttcagactga
    17221 caggattctg aaaaaagcag aaagattata attatacttt ttagtgcagt tgattttcaa
    17281 aaggaggaaa agaagatgaa aaagggtaga atattgagac tgatgtttat tctggcagtg
    17341 acaactatta tgatcgctgc aacagcagtg gttgcaagtg cagcgacagt taacaaaaat
    17401 gatgcagatt ataagtattc caagacatac gacaacggtg tgaagatatc ctttacaaca
    17461 gatgttttcg gcaagaaggt tgttgacgac agcgtttttt gcagaataga gctgccagat
    17521 ggtgcaaact ataaagaatt tagtgtctct aacgaaatca ttaaaagtgg taaagctttc
    17581 gtagattaca cgcacctaag agacagatat ccagtagaaa tcaaacagaa gtgggtgacc
    17641 aatgggaacg tttcatacgg aatgattgga ttaagtgcag gaggcggcct gtacctagga
    17701 acctttaagc tgaaagttta tgtagaaact ccagataaag atgaaatgat agaagttgca
    17761 tttctcagcg atttcgcagc tcgtccagtt gatatgaaga ttactacgta tcctaacaaa
    17821 ttcaatatca actcaataaa tgtaagtgct gcagacagcg gaagaacttc tcgtctggaa
    17881 gtagttggag taaacaaaaa ttttgcaaca attataaatg gctggaatga tggcaagtgc
    17941 attaaagcaa aaggaatgaa acctaaaaag aaatacactt tcaagttcta tggaaaacta
    18001 aaagtagata atcctgctct tgatgcacct gacggtataa ctgttgaggg aaatccggcg
    18061 gtattcaaaa atgttgaaat gggacctgca gtaaaaccgg taatcaagtc cataaaagtt
    18121 tcaaatgtaa aggtaacaaa gtatttcaat tacagcgagt ggagatataa atataagaca
    18181 aagttcacag ttaccgtaac tcttggcaag aagccaaaag gtgccaaggg tattcagttg
    18241 acaacaactg ttcagggaat ttcctcatat aagacaatta aaggaaaaaa gaacaccttc
    18301 acagcgaatt ttaattggga tgcaccggta agcttaaagg gcagaacagc aagcttcaag
    18361 gttaagactt acaacaatgc caagtacaaa gcttacagct atgactcaaa gcctaagaag
    18421 ttaaagatca aataggcata gtatacagat ctaccggaaa tatgtcatct gatgtgaaag
    18481 tcacagttat ggaaaaatgt cagtaaaaat tcctatggaa aaaataaaac atatattcca
    18541 tagaagaaaa gtaggagaac gaatataatg atcccaagtt gtttgaagga ggagatcatt
    18601 atgagtagtc aaagaaaggc agaagctgtg gcatcagaga atatcgtaac aacagagaca
    18661 tccagtctga gtgatctttg gaaaaaagag gattggctgg cagtatggat cggatttatc
    18721 attatcgcga tcggtgcagt tgcagtactt acaggagcat ttgattttaa tgccgcaaaa
    18781 ttcagcacct ggggtcatgg aacaagtttt tttgaacagg taaacgcagg atttttcgga
    18841 aaactgattc tgacagtggc ggtacttgga gtactgttta ctgttggaaa ccacctgaag
    18901 ggattatccg ttaaaaaata ccttccggca tttatgggac tgtttcttct ggcagttctg
    18961 gtaagattta tcagtgccga gtatacattg aaccgttatc tggaatgggc attttttgca
    19021 atcctgatcg gaattctggt atccaacacc gttggagttc caacatggct gaagccggcg
    19081 atccagacag aattctatat caaagtcgga ctggtgatca tgggattttc cgtactgttt
    19141 tccaatattg ttaattttgg actttacgga ctggggattg catggatcgt aacaccgatt
    19201 gtcattcttt tcatgtggtg gttcggaacc agggttttaa aaattgacaa taaaccgttt
    19261 gtcattaccc tggcaaccgc aacctctgtg tgcggaacgt ccgcagcgat cgcagtcgga
    19321 gcagcaagta aagcaaagaa aaatgacctt tcacttactg tatctatatc aatcattttt
    19381 acagtactga tgatggtatt tgagccggtg ctgatccgtg taactggtct tggagagctg
    19441 ataggcggtg ccctgatcgg tggtactgtc gattcaaccg gagcagtaac cgtagcaggt
    19501 actgcacttg gtgaaacagc acagacggct gcagtccttg taaaatccat ccagaatatc
    19561 ctgatcggat ttatcgcatt tgcagttgca gtattttttg caacaaaagt tgaaaatagc
    19621 ggagaaaaca gcagaaaaat cggggtatcc gaaatctggt acagacttcc gaaatttatc
    19681 cttggatttt ttgccgcttc cttaatcgca tcttttatag cagttccggc attcggaaaa
    19741 gagagcgttt catccatcaa cagcgtactg gatcagtata aaaactgggc atttgttctg
    19801 gcatttacaa gcatcgggct ggaaacaaat ttccgtgaga tcaaagaaca gttccaaggc
    19861 ggaaaaccac tgacacttta tgtggttgga cagttgttta acattgttct gacttttgcg
    19921 gcagtatatc tgctgctgag cggaaaattc ttcccgctgc caaatctggc attataagga
    19981 gatgcagact atgaagaaaa aaacaggaaa aaagaaaaat ctgttttctg tcgtaaatta
    20041 taccgttctg gtgctgacgg tcattgcagc cgtttatatc atggcaaatg gtctgggact
    20101 gatcgacagc ctggactttg gggcaggggc atattattat gcggatattc ctcagtttgc
    20161 aaaatatgta gatggatccg gctacacatc accgatttcc atgtgggtgc tcattgcact
    20221 gttcctgatc tggggattct tcatgtataa actctgggga tggattgaaa gaaaagataa
    20281 gtaaaaataa ggtgaaagaa aagcctgtcg cagagtatgc ggcgggcttt ttcaattcgc
    20341 agatattgat tgagagaaaa aggttgcctg gaggtgtttt gtcgttatca taaaaaaacg
    20401 gcaatttttc agattgccga ttgatgcagg aggaacggaa atgccgccag ggcagaagaa
    20461 acaaaaacgt tgacaaacat cacacagaga gttatcataa atacataaag gttctgaaac
    20521 aaatctaaat tcattgccgt tcggcaggaa aatccagaaa agagacctgc acgaagcggc
    20581 agaaacagga atcatgcaac catagcagct tcgtgccaaa aacagaagaa aaggagcgaa
    20641 gcctatggga gaaaaagaca ttacggaaaa aatatcaaat ctctgatgga aataccgaca
    20701 ggattagatg aatttgtgag tgattatcag ataaaagtat tcgaagtcgc ctggctgagt
    20761 gaagagcaga tccgtcgatt tcgaagtgat tttcggattg tggcaaactt ttttgtaaac
    20821 aaaagaaaaa acaagaacta tataccggat gatccgacag aaatacagca tgtagatgaa
    20881 gtgctgaaac ttttgcaggt catgaccgga gatgcaaggt ataaaagaat ttttggcaaa
    20941 aagaaggaag gagtgcacag tatgtgtgat gtagcagaaa gattagaaaa aatgggatgg
    21001 gagaagggac aaatggaagg aaaaatagaa ggaaaaatac tggtatataa aaatctgcgc
    21061 agggaaggat ttgacaagaa agaagccaga agacttacag agctgccaga agatataact
    21121 ctggaacagt aatattgcgg tgtcagaatg gatagtaaaa tgagcgtggt tattactttt
    21181 cgcaattgaa gcaggggact tacttgattt gattaaataa aaatttacaa ccacccatcc
    21241 agtgtgataa aatgtgaaag aattatgata aaaaatgtcg aaaacaggaa aatttcaatg
    21301 aatcatagaa gagaggaagt acaaatgatc actgtaaaaa cttatcgagg acatattcgc
    21361 aactggaagg aactctgtgc agagctggag attgatccgg cactgccaag agaagaaaaa
    21421 gagcaggaaa ttctggtaaa agcataccag aaatggggag gcgagatggc agaccatatc
    21481 tatggtatgt ttgcttttgc cctgtgggat gaagaggaga aaaagctgtt ctgcttaaga
    21541 gatcagtttg gaacaaaacc gttctattat taccagacag aagacggtga acttctgtac
    21601 ggcaccatga tccgtgacat catcggacag ccgggattta aaaaagagct gaatgaagag
    21661 atgctccaga tctatatgag cctgacctat ggtgccggtg aggatacctt tttcaaaggt
    21721 ctgaaaaagc ttctgccggg acgttatctg atctggaagg atggaaaagt ggaaattcac
    21781 agatactgga ctccaaagtt tcagccggac gagaacaaat ctctggaaga ctgggcagat
    21841 gagatccatt ctacgctgaa atccatcatg ccggaagtaa agacagagga cgaaaccgta
    21901 gaatctttcc tgtcaagtgg ggtggattct tcctatgtac tggcaatgtc agatgcaaag
    21961 atgtccgatt cctgcggtta tgatgaagaa cgatttgatg agtcacgtct tgcaaaaaag
    22021 acagcggaac ttctcggaag agaacattct gtctgcaaga tcacaccaca gcagtatttt
    22081 gatatcgtac catatgttat gtacaatatg gaacagccgc ttggagatgc atctgcgatc
    22141 gtatttacca ttgcctgcaa tgcaaccgca gaacacacga agatttgcta ttccggagaa
    22201 ggtgcggatg aattcttcgg tggttacaat atgtaccgca atgcagaacg ttacggtgaa
    22261 aatctgaaga tattttatgt cggcaataca aacatcatga aggaagatga aaagaagaaa
    22321 atcctcaaac actacaatcc ggaagttctt ccgatcaatc ttacgcatga aatctacaaa
    22381 gagacggaag gtcttgatcc gcttacaaaa atgtccgatg ttgatattca gatctggctg
    22441 gaaggcgata tctatctgaa tgtagataag atgagtaccg cagcagggct ggagatccgt
    22501 atgccgctta cagaccgccg gatctttgat attgcatcaa gaatgccgtc ccgttttaaa
    22561 gtcaacgaag agcagaacaa agtggcattc cgtaccgcag cagcaaaagt actcccggaa
    22621 gagatcgcat tccgtaagaa actgggattc attgtgccga ttcgtatctg gatggccgat
    22681 gcaagataca atcaggatgt acagcgtctg ttccacagtg agatcgcaga gaaattcttt
    22741 gatgtagatg cgatcaacga gattttcgat gactatgtag gaggcaattc cgataactgg
    22801 agaaaagtat ggacgattta taccttcctt gtatggtatg aagaatattt tgtaaaaaga
    22861 taattcttaa ataacatttt tccccctgtc ttctggtaaa agcagaaatg ataagctggt
    22921 tttatcagaa aaagaaagac ctgtattaaa tggaaaaact ccatgaaata caggtctttt
    22981 gactctggca tcctattgtg ttttcaggat tttgtttttt acattctttt acttcataga
    23041 agctacttta tcgaactcat cctgaacttc tctggaagga gccggtgtca ggagacttac
    23101 gattacgttt actgcaagac caagaaggaa tgcaggaagc agttcgtaaa taccaagtgc
    23161 accgcccatc ggacggatca gaaatttcca tacaaatacc ataacgccgc cgctgaccat
    23221 tccgcagaga gcaccctgga atgtggagcg tttccagaac agtgccagca gaacaagcgg
    23281 tccaaaggaa gcaccaaagc ctgcccatgc aaaggatacg atctggaaaa cagaactgtc
    23341 cgggttacgt gcgataaata caccgatcag tgcgataaca agaacggtga tacgtgcgat
    23401 cagcataccg gtcttttcgc tcatgtcttt cataaatact tctttcagaa tgttctgaga
    23461 agcactggaa gaagcagcga gtagctgaga gtccgcagtt gacatggtag ctgccaggat
    23521 accggcgagg atcagtccgg caatgatcgt tgccagaaca ccatgtttgg agatcagatc
    23581 tgcaacaacc agaatgatgg tttccggatc ttttaaagtc ggcaggacac cggcttttgt
    23641 catagcattt ccaatcacac cgataaatac ggaaacagcc atggagatca caacccagac
    23701 ggttgcaact cttctggaga gtttcagttt gtttgcatca ccgatcgcca taaaacgaag
    23761 cagtacgtga ggcataccga aatatccaag tccccatgcc agtgtagaga aaatctgcag
    23821 cagactgtaa ggagaagctg ttccggtagc gacatcgtgc gtgtgcgtca ggctgaagta
    23881 tccggccatg gattttgcat tgtccataac ggcactgaaa ctgccgactt tgctgatacc
    23941 aaaaccaaat acaacaacga tcgcgatcgt cataacaata ctctggacaa agtctgtcgt
    24001 acttgctgcg aggaatccgc ccagtgtggt gtaagctacg ataacgatcg cactgatcag
    24061 cattgctggc agataatcca ttccgaacag gtttgcaaac agcttgccgc aggctgcaaa
    24121 tccggaagcc gtataaggta caaagaaaat gatgatcagc agtgctgcga ttacggaaag
    24181 aatacgtttg ttatctctgt aacggttgga aaagaacaga ggcagtgtcg tagagtttcc
    24241 gcagacatgc gtataattac ggagtctctt ggcaacaaca agccagttta cataagttcc
    24301 gattgccagt ccgattgcag tccacgctgc atcagccgta ccggacaggt aagcaacgcc
    24361 aggcagtccc atcagcagcc agctgctcat gtcagaggcc tcggcactca tggcggtgac
    24421 gagcggtccc agtgtacgtc cgccgagata gaagtcatct gtggtctgat tctgttttgc
    24481 cagaaccgca ccgataccta ccatcgcagc caggtagacc aggattgtga tcaacattag
    24541 aatctgtgtt acatccattt taaatcctcc ctaagtttct gcagtttgat gttgtcattc
    24601 acatatgcgg aaaatgaaac atccggttta tgattaacgt tccgtacgga agagcagtcg
    24661 aagcagtctt tgtacggatt gtttgtcagt tgagacttta acacagtgca gtgtcagcga
    24721 ctcaaaatga cactataaac caaatattaa cataatttag gcgaggctac aataataaaa
    24781 tactaaaaac ctgttataaa aagcccgata tttaaaaaag ttacggtcag agcggtaatc
    24841 caaagaacga tattcaggcg tctgctgttt gcatatctgc ccattaccct tttagaagaa
    24901 gtcagacaga tctgtgtgaa gatcgtaatc gggagttgaa cggcaagcag catctgtgaa
    24961 tagataagtc cctggaacgg cgaagaaata aacaaaatga tcacagcagc cggaaccatt
    25021 gtcaccagaa tccccagttt tgtttcttta ccggaagtat cataaggctt gttaaaaata
    25081 ccggaaaaaa tcgtaccgcc tgccattccg gctgtgatgc tcgaagaaat cccggaaagc
    25141 agcagagcga ctgcaaagac cagtgatgcc atatttccaa gcagaggagc gagcatagaa
    25201 ccggtgactg caagatcgtc aacagtcgtt ccatttccat tctgataaaa acttgctgcc
    25261 gccatcagga tcatggcact attgatcgcc cagccaatga tcatggaaaa cagcgtgtca
    25321 cggaattcat atttcagctg cttttgaatt acagcatcat ccgcaagaga ccactggcgg
    25381 ctctgaatga tttcagaatg cagaaacaga ttgtgcggca tcacaaccgc accgagcaca
    25441 ctcatgatga ccggcatgga atgggcagga aacgaaggct ttacccagcc gatggcagcc
    25501 tgtttccagt cgattcctgc gatgcagatc tcaaataaaa atgaaaatcc gatcagcgag
    25561 acaaaaagaa tgatcaattt ttcaatccgc cggtaggcat tggtaaacgt acagaacaaa
    25621 acaatcacga gaatcagcag actgcctggt ttgatcggga ttgaaaaaag catctgcaga
    25681 gcgattgcac cgcccaggat ttccgccata gctgtggcaa tcgaagcaag cattgccgta
    25741 gacagaatga tgttcttgag aattcggttg atgtatgtgc tggcagcctc cgaaagacaa
    25801 agtccggtaa caatgccgag atgggcggca ttatgttgca gaaggatcag catgatcgtg
    25861 gagagcgtga ccatccacag aagctgatag ccatacccag aaccggcggc aatgttcgaa
    25921 gcccagttcc cgggatcgat aaaaccgacc gtgaccagaa gtcccggacc aatgtactta
    25981 agaaaattta gtggctgctt tttgtgtttc tgtatcataa aatacttccc cttttccact
    26041 tctttaaaag aatatgccca tttttgcaga acagtgagca gattccctca gaatcttgtt
    26101 gcaggtatca tatagcagta cctgtagttt tatgaagtta gttataacta actacgtgca
    26161 tttattatat gcacagaaac ggaagcttgt caaaagaata ttcgcttgtt gcgaaaaata
    26221 gacgaaaacc gtataattat caggtagata atttaaatga aattttccgg acggagcctt
    26281 aaatgttatg agcaagtagc aaagcggatt gcgaatatcc gcttgacaat atatagaatg
    26341 aaaaggaagg aaaacaaaaa atatggttac aggggaatta aaaaataaag tagacggttt
    26401 atgggaaatt ttctggacag gcggactgac aaatccgctc gatgtgatcg aacagatgac
    26461 ctatcttatg tttatccgcg atctggatga ttcggataac gtacatatga aagaaagcat
    26521 tatgcttagt ctgccatata aaagcatatt tgaagaaaac gtaacaatcg gagacagaaa
    26581 agttccgggg aatcagctca agtggagtgt atttcacgat tttccggctc agaaaatgta
    26641 tgcaaatatg caggaaaatg tgttcccgtt tatcaaaaat cttcatggag ataaagaaag
    26701 tgcctatgca aaatatatgg aagatgcgat ctttaaagtg ccgacaccgg tcatgctgga
    26761 taagatcgtg actgccatgg atgaaatcta cgaaaaaatg gagcagttaa gaagtgaaga
    26821 tgtccgggga gatgtatatg aacatttact tttgaaactg aaaactgctg gaacaaacgg
    26881 acagttccgt acaccgcgtc atatcataaa aatgatggta gaaatgatgg atccaaaagc
    26941 cgatgacctg atctgcgacc cggcctgcgg tacctccgga tttctggtag agtccagtga
    27001 atatctgcga aaaaagaaaa aagcagaagt tctgtttaat gcaaaaaaca aggaacacta
    27061 tatgaatcat atgttccacg gctacgatat ggacagaacg atgctgcgta tcggagcaat
    27121 gaacatgatg acacacggtg tcagcaatcc gaatatcgaa tatcgagaca gtctttccga
    27181 ccagaacgaa gataaagaaa aatacagcct gatcctggca aatccaccat tcaaaggaag
    27241 cctggattat gatattgtat cggcagatct tctgaaagtc tgcaaaacca aaaagacaga
    27301 acttcttttc ctgacactgt ttgtaagaat gttaaaaaca ggcggaagat gtgcgtgcat
    27361 cgtaccggat ggcgtacttt tcggttcgtc caaagcccat aaagatatcc ggaaagcaat
    27421 cgtggaagaa cagcgactgg aagcagtgat ttccatgccg tccggtgtgt tcaaaccata
    27481 tgccggagtt tctacagcga tcctgatctt tacgaaaacc gggcatggcg gaacagacaa
    27541 agtgtggttc tatgatatga aagcagatgg acgcagcctg gatgacaaac gttctgaagt
    27601 caaagaaaac gatattccgg atatcatcgc aagatttaaa aatctggaag cagaaaatga
    27661 ccgtaaacga actgaccagt cattttttat agaaaaacag gaaatcgtag acaatgccta
    27721 tgacctttcc atcaacaaat acaaagaaat agaatacatt ccggtagaat atccgccaac
    27781 atctgagatt atggcaaatt tacgggcact ggaaaaagaa gtacaggaga gcatggcaga
    27841 acttgaaaaa atgttaggag atgagatggc atgaaaatag aacatatcgc aatgtatgta
    27901 aaagatctcg aaaaaacaaa aacatttttt gaaacctatc tgaatgccag agcaaacgct
    27961 ggttatcaca atccgaaaac agactttcgc tcttacttcc tttcctttga cgatggggca
    28021 agactggaaa ttatgaataa gccgcagatg caggattctc cgaaagccct gacacagacc
    28081 ggatatattc acattgcatt cagcgtcgga agcaaagaaa aagtagatga gttgacagca
    28141 aaattaaaag aagatggcta tgaaatcatc agcggtccac ggataaccgg agacggatat
    28201 tacgagagtt gcgtagtggg aatcgaaggc aaccagatcg agattactgt gtagcaaagt
    28261 gattttcacg ctccacacag ctaaaatata gaatatcaac agagcaaatc ccacgagtgt
    28321 aaaattaact ttgtgggttt tgctcaatcc atttttgaat ggtttttaaa tcaattttta
    28381 aagcagtagc aatttgctca acagacaagc caagatcaac taaggaaaga gcatcttccc
    28441 ttttaatatt ttcggtagct gttttgatac catcagctac cccagcctca tattccgcct
    28501 tacggagttt gcgttcttcc agttctttat catattcaaa tatactcatg gagatcacct
    28561 ctgctttgtt tgcacgaaga aagtcggaaa gaatgttgtc atgtatacat tcattgacag
    28621 ccagttcaac cgcttcgttt aattcaaaat ctttcgtgta tttgcgtaca agatttacat
    28681 attgtgcata ttcctttaaa gtttggcatt gttccatcag ttctttatta tgtccctcat
    28741 tgatattcag tgtcagaact tccagttcca gctttggatc accggattgg ttttcgaaag
    28801 cttcggataa atggtttacc cagtagtcct ttttcttttc cagaccattg taaaatacaa
    28861 taaattgcgg tgccggtatt ttctgtaata cagaagaata gagagatttg tgatctacta
    28921 atttctggta ttcactggaa atatagaata aatcacgcag cggcatattt ggatt
//
LOCUS       DAWDWH010000006        26980 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_1075_length_26980_cov_15.731625, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000006 DAWDWH010000000
VERSION     DAWDWH010000006.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 26980)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 26980)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..26980
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1075_length_26980_cov_15.731625"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    11158..11167
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12781..12790
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         8064 a         5670 c         5768 g         7458 t
ORIGIN      
        1 cgtacatggt aaataccacg ccttcgattt ccagatccgg gttcagtcga tcctggacaa
       61 gattgattgt gtggatcagc tgagacagtc cctcgagtgc ataatattcg cactgaatcg
      121 gcacgagtac cgtatctgcc gcacacattg cattgatagt cagcatactt aaagaaggtg
      181 gacagtcgat cagcacaaag tcatagtttt tctttacttt atcgattgct ttctttaaaa
      241 tatattcctt ttcctctgct cccaccagct cgatctctgc tgctgccaga ttgatatttg
      301 acggaagaac actcagattt tcatactcac tttccataat cgcatcctct acggaacact
      361 cttccagcat cagctcgtac accgtatttt ctgcctgctc tttgtcaatt cccagtccgc
      421 tggtagtatt tccctgtggg tccatatcca ctaccagtac cttctttccc agttctgcca
      481 gagaagctgc cagattgatc gctgttgttg ttttgcctac accacccttc tggttggcga
      541 ctacgatagt tctttccatt aaatattccc ctttcgtttc cagttcatac cagatgctgc
      601 tttgtttcac gtgcatttgc cctgaacaca aagtattttt caattatagc acttcttttt
      661 ttagatatcc agtcttaagc attccaaaat cctggaaatg tttcacgtga aacatatgaa
      721 acattaatcc ggcagattct gcacctcttc gagcatgtac agaaacttgt ccagctttgc
      781 cgttacctct tccagttccc tcgctgtctg tgcctgctgc cttttgacat cattcatctg
      841 ctgatgcatc tcattcagca tctccttaga cacacgcata tctgcccgcg gcgaaaaaat
      901 ttcaaaatca acgtcttcct ttttttgcac ctcgtctatg cttttgtgaa ggcgctcacg
      961 tatcccgtca agcctttcct tttgagctgt caaccgttct ttttcggctg tcagactgtc
     1021 tgctttttcc cttaaatact cttttaccat tttatcatcg ctttctgtat gttctaaccc
     1081 attgtactgg ataaacagaa ttatgtaaag caatgtttca cgtgaaacat atgaaatttt
     1141 tatatcttct atattgtttc ttatttcaag aatacctctg acattcttgc tgcgatgtgc
     1201 acatcatgca tcctatgcca tattcttctg tgcacatctg atatactttt gccttgtctc
     1261 tgcttacatc ttggattata atgaaggcaa tgaaaaatca caaagatctg tataagacaa
     1321 tgtttctcag aaaggagttt ttatgaagca aaaaatcaaa accggcattc tcatgaccgc
     1381 tctggctgtt ggctctcttc acatcgtcaa tcgctgtgcc caggctcttg ccacggttaa
     1441 aaatctgctg gataaacata aaggtgcttt ttatgactgg cgtttcggtc aggtctatta
     1501 tacaaagaca ggaagcggtt ctcctctcct gcttgttcac gatctggctc cggctgcctc
     1561 ttcctacgaa tgggatctgc tgctgaaaaa tctgtccgca gaccacacgg tatattgcct
     1621 ggatcttctt ggctgcggtc gttctgataa accgaacctg acctacacaa actattttta
     1681 cgtgcagctt atcaatgatt ttatcgagca tgtgattcac gaaaaaacag acatcatcgc
     1741 taccggtctg tcttcttctt ttgtgttgat ggcatgcagt accaatccgg atgcctataa
     1801 caaaattatg atgatcaatc cggaaagcat ttccaatctg aaatgtacac cgtccaaacg
     1861 ttccaggatc atcaaattta ttatggatct tccgatcgtc ggtacagctg tatacaacat
     1921 gcagatgcgg cgggaaaata ttgaatattt attcaccgaa aagtattttt ataacccttt
     1981 ctctgtctca aagaagacgc tggacattta tcatgaagct gctcatctcg atgagtgcgg
     2041 cgggaaatat ctgctgtcca gcctggatgg attttattta aatgcagaca tctccagagc
     2101 cttaaacagc atcaacaaca gtctgtttat cctcgaagga agaaagacag aggaacgtga
     2161 acgaaccgtc gaaggctatg tccgcctcaa ttccgctgta gaatacgatt ttattgaaaa
     2221 tacagaaaaa tacccgcaaa tggaaaatcc gcaggctgta ctggaatatg ttgacatgtt
     2281 cttttaaaat tttaatttga atacacgtaa gatctggcac aaaaccatta tacccggaca
     2341 tttctgaatg tctctgacat tcttttgtgc acttctgcaa tccgccgaag gttatatcag
     2401 atgggagatt ttatcccgtc acagacacag acttgttgtt caccgcctgc aaagacggag
     2461 atcacctcac atctggtgta caccttcgct gctgggttta tcttacccca aaatcatcaa
     2521 acagattatc gttctcgccg atatgataat tcctggcaat atctactttt atttcccctg
     2581 tctatacaaa agttctcaga tgattttttt tctttttgta atccacatag taaagccggt
     2641 cggaaagcca ttcttcctcc ggaatggttt ctctgtttgc ccgttttatc tcatcaataa
     2701 aaaactgcgg atcataatcc tccggcaatg tattttccgg aatgacaacc acgtggtgaa
     2761 tgttccatag ctggatcagc aaattgcatt tgaatttcat atacatccat gcctgcacct
     2821 gcggaagtgc aatcgccgct gctccatagg aatagtacag attggacagc atataaattt
     2881 ccatagtaca actgtaaatt cgatcaatta catccgatgg tttcacctgt agaaaacagt
     2941 gcgtaaaata atacagccat aaatatccga aaacaaattg cacggacggc agcttcccag
     3001 gaaacgaagc gtgtcagcag attgttttcg tgtatttatg gcgtactgtt ttttccctat
     3061 ttttaactat cttttactat tttaatgatc ttctttttgc acattcacgc aagaggctct
     3121 ttcgttggca tcccggcttt tctcgggtat ttcttcggcg tgcttctctt cttttccaca
     3181 accacaagtg ctctctccat gtcacttccc ggcagatgga atttctccac ttttccgatt
     3241 tctccgccaa gcacataaac tgccttctta ctctgagcga tttcttcatc cactgaaaca
     3301 gatttatagg aaacaaattt tccgccgact tttacaaacg gcaggcaata ctcggtaagg
     3361 gacgcaaggt tcgcaactgc tctggaaaca gacagatcaa actgttcgcg gtattctgct
     3421 tttttcgccg catcttctgc tcttccatgc accgtttcga ttccgataag ccccaggtta
     3481 tccacaactt catccagaaa tttgatcctt ttgttcaaag agtccaaaag tgtcacctgc
     3541 aaatgtggat aaacaatctt cagcgggatt cccggaaagc cggctccggt cccgacatcg
     3601 atcaggctgt ttattttttc catctccaca tattctgccg ctgccacgct gtccacaaaa
     3661 tgtttctgca tcacttcgtc aaattcagtg atcccggtga ggttcatcac tttattccat
     3721 tctaccagca gctcataata tttctcaaac tgttcaatct gcttctctgt caatgtcacg
     3781 ccaaatgcct gacatccatc ttctaaaata tgaaatttac ttctgtccac gttttcctcc
     3841 tgtcaaatat ctcgtgcatc tgacttaaaa ttcaatattt acgtttcgtc tgccaaacat
     3901 tcttttgccg caggacttca ctttattttc gatctctgga ttccaaatat acgagcagta
     3961 cggaaatatc cgccggtgac acaccggaaa tacgggatgc ctgtccgatg gaaagcggct
     4021 ggaatttttt cagtttctgc accgcttcaa ttctcaggct tccgacttcg tcgtaattaa
     4081 tatctttcgg aatccgtttg ttttccattt tcttgaactg ctcgacctgc ttcatctgtc
     4141 ttcggatgta gccctgatat ttgatgttga tattgacctg ttcacgcaca ccggcagaat
     4201 actgcggtct gtttttgtca atctctgcta acatctcata gtccagctcc ggacggcgta
     4261 ccagttctcc gatggttgtt ccggacttca gaagtgtact ttcatgtgcc tccagaaatt
     4321 tctgtactac ttcggttccg ccgatattct catgatctac acgttctatt tcttctgcga
     4381 tctgacgctc ttttgttttc agatcctgat agcgttcttc ggagatcagc cccacctgat
     4441 aacctttctc tgtcagacgc agatctgcat tgtcctgacg cagaagcaga cgatattctg
     4501 ctctggatgt catcatacga tatggctcac gattctcttt tgttaccaga tcatcgatta
     4561 gaacaccaat ataagcttcc gaacgatcaa ggatcagcgg ctcttttccc tttacctgca
     4621 ttgctgcatt gataccggca atcagtccct gtgccgctgc ttcttcatat ccgctgctgc
     4681 cgttaaactg tccgccactg aaaagtcccc tgatattttt aaactccagg gatgcatcca
     4741 gctgtctcgg attgatgcag tcatactcga tcgcataggc attgcggacg atctttgcgt
     4801 gctcaagtcc ggctaccgtg tggtacattt catgctgaac atcttccggc agggagcttg
     4861 acataccgcc gatatacatt tcgttcgtat gcagtccttc cggctcaaga aatacctggt
     4921 gtctgttttt atctgcaaat tttaccactt tgtcctcgat ggacggacag tatcttggtc
     4981 cggttccctc gatcataccg gaatataggg gggaacgatc caggtttttg cgaataattt
     5041 catgtgtctc ttcgtttgta taagtcagcc agcaggatgc ctggtcgatc tggacactct
     5101 ccggatcggt tgtaaaagaa aagggtacca ctctctcatc gccaaactgt tcctgcattt
     5161 ttgaaaaatc aatcgatctg cggtctacac gtgccggtgt accggtctta aatcgctgca
     5221 tctcgattcc atgtgctttc agggaatctg tcagatgatt tgctgcctgc aatccgtttg
     5281 gcccggtata attactgata tcaccgtaaa tacatcttgc acgcagatat gttccggtac
     5341 aaagcacgca ggctttgcag tgatacgttg ccccggagaa tgttttgact ccggtgattt
     5401 ttccgtcttc tgtcagaatt tctgcaactt ctgcctgcac gattgacagg ttatctgtgt
     5461 tttccaaaac ttttcgcatg gatctggaat attctgcctt gtctgcctgt gcacgcagag
     5521 aatgtacggc aggtcctttg gatttattga gcatcttgga ctggataaac gttttgtcga
     5581 tattttttcc catctcaccg ccaagtgcat cgatctctct taccagatga cccttggagc
     5641 ttccacctat gttcgggttg cacggcatca gtgcaatgct gtccacactc acggtaaaaa
     5701 tggttgtgga aagtccgagt cttgcacagg caagtgctgc ctcacatccg gcatgtccgg
     5761 ctcctacgac taccacatct gtatattctt ctaatgtatt catttcttgc ctccacttat
     5821 ttgcccatac agaacttact gaaaatttca tttaccagat catcatccac ggcctcgccg
     5881 ataatcgttc ccagagattc atatgcactc atcagatcga tcgagaagaa atcctccggc
     5941 atatcctgtg cgatgctctc cgtcacctgc ttcaggctct cttttgcatc cataagtgct
     6001 gccttatgac gcatatttgt gatacatacc tcatcgttaa aagaaaggct gccttcgaga
     6061 aacatatttt tgagtgtatc ctcaagctgt tcaaatccag tctcatcttt tgcagaaacg
     6121 cagatcaccg gatgttctcc cactttttcc ttcaggatct gttctgtcgt taccggctcc
     6181 agatccgatt tatttaaaag aacgatcacc tttctgcctt caaatagttc gataatctct
     6241 tcatcattct catccagtgc ggtcgatgaa tctgccacgt acagaatcag gtctgcttct
     6301 tttgcatatt tttttgcacg ctgcacacca atctgctcta cgatatcttc cgtatcacgg
     6361 atgcctgcgg tatccatcag gttaagtgtg atgccgtgaa gattgatgct ctgctccagc
     6421 acatctcttg tcgttcccgc aatgtctgtc acgatcgcct tttccgttcc gcaaagcttg
     6481 ttaagcagag aagactttcc ggcgtttggc tttccaagga ttaccgtctt gatgccttcc
     6541 gtcatgatcc ttccatcgtt tgcagagcgg atcagcttct cgatcatctc gatccacgct
     6601 tcattttcct tttcaagctc ctgcggataa ccgtcaaggc tgatatgttc cggatcatcc
     6661 aatgccgact caatatatgc aatatgataa atgatctttg ctctcagatc ctttattttc
     6721 tgaccgatgc ttccgcgaag ctggccgacg gaacttttca gagcgtattc attttttgcc
     6781 tcaatgacat ccatcaccgc ctccgcctgc gacagatcca ttcttccatt taaaaaggca
     6841 cgtttggtaa attctcccgg ctctgccgga gctgcaccat ttttgatcac agcttccagc
     6901 actcgctgta tcgcatacac accgccgtgg cagtcgattt ctacggtatc ttccgctgta
     6961 aaacttctcg gtgcacgcat cagaactaca agcacctcat ccagcacttc gtctccgtca
     7021 cagatataac catagtgaac cgtatgggat tgctgttctg ataatttctt tttgcctttc
     7081 ggacttctgt atacacggtc cgctatttca aatgcctgat ctccgctgat tcgcacaatc
     7141 ccgattccgg aactgctgac tgctgtggag atcgctgcaa tcgtttcacc tttcattgat
     7201 tcctcctgat attcaaaaag tctgattttc ggcagcatat ctgccgcact gaaaatcaga
     7261 ctcatatcac ttacaactat tttactttac tgttttttta atgtaacaac tactttacga
     7321 tacggctctt cgccctcgct gtgtgtggac accagcggat cattctgaag tgcagaatgg
     7381 atcactcttc tctcgtatgg attcatcggc tccaggaata ccggtttctt tgttctcttt
     7441 actttgaacg caatattctt tgccagattt tccagcgtct cttttctgcg tgcacggtag
     7501 ttttcggtat ctaccttcac acgtacatat cccgcattgc ctttgttgac aacgaggctt
     7561 gtaagatact ggatagaatc aagagtctgt cctctcttac cgatcagaac acccatgtct
     7621 ttaccggaaa gctcgatgtt cagattgtca tctttatcaa atgatgtttt gatgcaaact
     7681 tctgaatcca ttgctgcaaa cattttctcc agaaattctt ttgcatcttt ctctgctgct
     7741 tctttgttga tcggaagctt ttctcttgta actgccggtt ttgcctgcgg ttcttctttc
     7801 tttacttcca cttctttttc agcttttggc tgcggctgat tttccggttt tctttccggt
     7861 ttcttctcaa ccggtttttc ttccttcttt tcaaccgttt tctcaggctg tttttttacc
     7921 ggctctttgg taaatttctt tgccggtttt ttcataaagc ttttctttgc agaaatgtcc
     7981 ttcaaaggat ctcttacggc ttcctcaaaa tcaagagaaa tatctttttt ctttcttgcc
     8041 cttataatgg ccggttttct tccgattcca aaaagtccgg aagttgcttc ctgaataaca
     8101 tcatattcaa tattatcgct gcttgttcca agctgaattg ctgcattcat gatcgcttct
     8161 tcttttgtct tcgcagaaac ctcgatatac tcttccattt taatacctcc catccttctc
     8221 cccatattac ttctctttat taccttatta ctttaattct tatttttctt accttttgga
     8281 ttcttttcat caaactgctg aaccattctc gcttttgctg caatacttcc cggtttcgca
     8341 tctgcgtgtt tatagtactc cgtagaatcc tggatttttt tctgacgttc tgcctgtttc
     8401 ttctcatcca ctttctgtgc atcctcaaga tctttgaagt ttacttttgc atgctcagaa
     8461 attttctttg gcggaagtcc ctgtttctta cgtttggcat tcattttctc cacatttttg
     8521 cggatcagtg cgtccatatc catcttttcc atatgtttgt tgacaacaac ctgctggata
     8581 cctctgaata ccgcacctgc gatccagtag ataccaagac cgatcggaag tgacagacag
     8641 aagaatacag ataccagagg catcatattg ttcatcatct tcatggagct ttccatggaa
     8701 ctgtcgtctc ctgccgggct tgctgctgcc tgcggcatca gtctggtgtt gagccactgg
     8761 cttaaacctg ccagtaccgg gattgccagt gcacatacaa ataaaatcca gttatgtgcc
     8821 ttgaaatttt ccaccatcgc tatcatcggt gaattggaaa tattcaatcc caggaaatag
     8881 ttcattttat caagactgcc tgttgtctgt gagatcatac ctgacagatc cggaaacttc
     8941 tctgccattg cagtccagtc tgccggtttg aacttgtaca gtgcatcgat cgcactgttt
     9001 gctgtataac caagtctctg gaaattgacc tggtttccta ctgtatcagc taaaaactgc
     9061 tgtgtaccct gtgtctttaa aagaacattt acaagaggag taaatacatc ttttacactt
     9121 gcaacgtaag ccggaatatt ccagattaca cgatacaatg caaacaggat cagcagctga
     9181 atcagcatcg gaagacatgt tcccatcgga gaaacaccgt attttgcata tacattctgt
     9241 gtttcttcgt tcatcttcat catacttacc tggtcgtttt tgccggcata ctttttctgg
     9301 attgccttta tctcaggatt catgatcgca gacatctttg aaaacttctg ctgtttgatc
     9361 gtgagtggag tgagcagggc ataaataacc agtgtaaaca aaataataca tacaccgata
     9421 ttggaaatcc caaacacccc gtcagtgaaa ctaaaaatcg cattcatcaa aagacctaac
     9481 agtgttgcca cctgtccaat gatgatggtg ttgctttttg ttaataacat aaccttcctc
     9541 ctgcatattc acctgacatt atacagcagt tcttcatatt caattttcta tttttaattc
     9601 ttcctacccg tttacggaac gggatcataa ccacccttgg aaaagggatt gcatcgcaaa
     9661 atcctccagc atgccagaag acttccttta acagccccgt atttttgtat cgcctccagg
     9721 ccatactgcg agcaggtcgg tgtatagggg catctcgtat gctttaatgg tgacagaaat
     9781 ttctgataaa accggatcat tgcgattaaa atatttttca tgaatatcaa cctgctattt
     9841 tatgcacttt accaagatgt aataatgcac tttctacttc aaaataattc ttatcttttg
     9901 caacaggtct tgcaattact acaatatcat atcctgagtg aaacctggtt tcactcaggc
     9961 gataactttc tcttatcaat ctggccagtc tgtgtctgac cacactattg cccacctttt
    10021 tactgaccga aatcccaagt ctgttcctct ctgtctgatt cggatagata tacataatga
    10081 gataacggtt ggcggccgac tttccatact tatataccgt ctggaaatct ctgttttttt
    10141 tcaaagactc tgaaaatatc atactgcttc cttttttctg tcatcctttc aaaagacgaa
    10201 catagaagaa aaggccacac atctgcggcc tatgctgata atttttttct tccttttaat
    10261 cttctggcag caagtacttt tcttccgcct gcagtgctca ttcttgctct gaatccatga
    10321 actttagctc tggatctttt ctttggctga aatgtcattt tcattattac ggcacctcct
    10381 tatcaaatat aaaatctgtt ttatctgttt agaaaacatc gtttcaatta tatatgcaaa
    10441 gccccataaa gtcaagtaat tccgtgattt tttcaattga accgagcttt ggggcagaac
    10501 cgttcctgtc agtcccatag acaaacggat gcaaagcgga catctgcata gtttacactc
    10561 agttatccac attttgggga taacttgggg ataacttttt tatttgatgt gaaaaacttt
    10621 ttttcttttt caaaaaaatg ccttaaaata aggcttttcc cactgtggat gatgttcctt
    10681 tagttattca cactgccaat cccccctctt tccttctttc gacatttttc tacattcttc
    10741 gaagtctgtg attcccaaaa ttcctttatt tttcaacctt tttttcatct ggttcttctg
    10801 taacatactt atgtaaactt attggtttac ataaagttat ccacattttg gggataactt
    10861 ggggataact tcgtgtgtaa cttttgcata ttttctacat ttttcccaca aacgcccatt
    10921 ttatcgcatt tttgagtgtg gattattgtc gaaaagttat gcacattcaa aaataagcat
    10981 tgataatttt cctgttttag tttaatatta aataagtaaa actggcctta aaaggagatc
    11041 ctcatgaata tcatagaaga aaagtgggac agtgttgtgg ataccctcag tacagaattc
    11101 ggattttccc caatttccat caacacctgg atcaaaccac ttcaagtaca aagatatnnn
    11161 nnnnnnnctt tttatttatg tcccgattga tgaaatgggt attaacgagg atttcattaa
    11221 ttcctatata aataagaagt ttacaactcc gcttcgttct acgattgcag agctcacagg
    11281 actggactgc accaagatcg tgtatactgt agaacaaaat gttgtaaaag aagcggattc
    11341 ctcttcgaat actgacaatt ctttcaataa tatattagaa aaagcaaatt taaatccgga
    11401 atataccttt gaaaacttcg ttgtcggaag caacaacaaa tttgcacacg ctgcatcttt
    11461 ggcagttgcc gaatccccgg gcgaaatcta caatcccctc ttcctctacg gaggtgttgg
    11521 actggggaaa acccatctga tgcactcgat cgcacacttt atcctggaaa ataatccaca
    11581 gaccagagta ctttatgtaa cgagtgaatc ctttacgaac gaactgattg aagcgatccg
    11641 aaacggcaat aatactgcta tgacaaaatt ccgtgaaaag taccgtaata tcgacgttct
    11701 gctgatcgac gatatccagt ttatcatcgg aaaagagtcc acacaggaag agtttttcca
    11761 cacgtttaac gacctgcatg gtgcgaaaaa gcagatcatc atttcatccg ataaaccgcc
    11821 gaaagagatc gcaacgctgg aaggaaggct gaaatcccgc tttgaatggg gcctgatcgc
    11881 agatatttct tccccggatt atgagacaag gatggcaatc ctccgcaaaa aagaagaacg
    11941 tgattccaaa gaaaaaggtt accatctgga tgatgaagtc attcagtata ttgctaccaa
    12001 tatcaagtct aatatccgag aactcgaagg tgcactgaac cggatcactg caatgagcag
    12061 tcttgaacac cgcgaaatta cagtagaact ggcacaggaa gccttaaagg atatgatttc
    12121 accgaacgca aaacgagaaa tcaccccgga tctgatcctt ctgaccgttg cagaacactt
    12181 tcatatcaaa acagatgata taaaaggttc caaacgaagt gctgatatcg tgattccaag
    12241 gcagattgcc atgtatctgt gccgtgaaat gaccagcaca ccgctcaaag cgatcggaaa
    12301 actgatggga aaccgcgatc atacgaccgt acttcatgga gttgagaaaa tttcgaaaga
    12361 aattgagact tccgatgatc tgaaaaacac gatagatatc attaaaaaga aactgaatcc
    12421 aaactagacg gccgaaaaag catcgtcttt ttatttgtgc acaaaataat gtggatatgt
    12481 gttggatact gtgtgaataa ctttagccaa tttctgagga tgttaataac ccaccggttt
    12541 tcctcagaaa tttgtggact tttcaccagt ttatccaacc tgttttttcc cttaaattca
    12601 aggattttgg gagttattca catattcaca tcccctacgg cggctactgc ggattttctc
    12661 tttaatttat ctcatttaac ccacaaagga gttatcaaca caatgaaatt agtatgctca
    12721 aaaagtaatc ttttaaaaag tgtgaatatt gttctgaaag caatcccagg aaaaacaacc
    12781 nnnnnnnnnn aatgtatttt aattgatgca tccacaaaca atattaaatt tacagccaat
    12841 gatatggaac ttggaattga aaccatcgtt gacggtacca ttctggaaaa aggaattgtt
    12901 gcaattgatg caaaaatttt ctctgatatc attcgtaaac ttcctgataa tgatgttacg
    12961 attgaaacag acggaaatct gacgactacc atcacctgtg agaaggcaaa attcagcatc
    13021 agcggtcaat ccggggaaga attttcctat cttccttatg tagaaagaga taaccatatt
    13081 gttgtctctc aatttacatt aaaagaggtg attcgtcaga ctattttctc aatcgcagcc
    13141 aatgaaaaca ataagatgat gaccggggaa ttattcgaag tcaaaggcga tatgctgcgt
    13201 gtagtatccc tggatggaca ccgcataagt atacggaaaa ttgaattaaa agaggaatat
    13261 tctgatatca aagttgtcgt tcctggaaag acacttatcg aaatcagtaa gattttatca
    13321 ggcgaaatcg aagatgaagt cagtattttc tttacaaaaa atcatattgt ctttgaattt
    13381 gatgataccg ttgttgtgtc acgtctgatc gagggtgaat atttccgcat tgatcagatg
    13441 ctttccagtg actacgaaac caagattcag gtaaataaaa aagagcttct ggactgtatt
    13501 gaccgtgcga cacttctggt aaaagaaagt gataaaaaac cgatcattct ttcaatcaca
    13561 gatgataata ttgaacttaa gattcagtct cagatcggtt ccatggatga agaaattgac
    13621 atcaataaag aaggcaagga cattatgatc ggatttaatc cgcgatttct gatcgatgct
    13681 cttcgtgtta ttgatgatga aacggttgat atttatctga tgaacccgaa agcaccatgc
    13741 tttatcagag acgaacagca atcttatata tatttgatct tgccggttaa ttttatggca
    13801 taaggagtga aagtttatgg aaacaattaa attaagagat gagtttatca aacttggtca
    13861 ggcattgaaa gcagcaggtt ttgtagagtc cggcgtggac gcaaaatatg ccgttcagga
    13921 tggtcttgtg aaggtaaacg gccagacaga aacacagcgg ggcaagaaac tgcatgccgg
    13981 cgatgtggta gaatatgatg gtcagactgt aaaaattaca gattgatgga atgatagtaa
    14041 aatctttgaa gctgaatcag ttccgcaatt atgagtcgct gcagttgtct tttgataagg
    14101 gaacgaatct tttctatggg aacaatgccc aggggaagac caatattctc gaagcggtat
    14161 atctatgcgg aacgacgaaa tcccataagg gaagtaaaga cagggaaatg atccgttttg
    14221 gtgaggaaga agctcatata cggatggcga tcagcagaag ccagagtgct tatgagatcg
    14281 atatgcatct gaaaaagaac cggccgaagg gcattgcagt caacggcgtg ccgatccgaa
    14341 aggcggcaga gctgcttgga atcgcaaact ttgttttttt ctcaccggag gatcttggta
    14401 tcattaaaaa cggaccggcg gagcggcggc gttttattga tatggaactt tgtcagctgg
    14461 ataagcttta cttacacgag ctttccaatt acaataaagt gatcatacag agaaataagc
    14521 tgctgaaaga gtgttctttt aacagtcagt atgaagaaat gctggatata tgggatatgc
    14581 agctggtaaa atatggaaaa aagctgatcg aatcaaggga gcggtttgtc gaagagcttg
    14641 gcgtgatcgt agcagagata catagaaatc tgacgggcgg cagagaagag ctgatggtcc
    14701 gttatgagaa aaatgtttct gcggacgaat tcgaaagaaa acttcagact tccaggaaac
    14761 gggatctgaa ggtgaaaatg agcatgacag gtcctcacag ggacgatctg ctttttgaag
    14821 taaagggtgt agatatccgc agatttggat cgcaggggca gcagagaacg gctgcacttt
    14881 cgctgaaact tgcggagatc gaactggtaa aaagagcgat caaagatacg ccggtactgc
    14941 ttctggatga tgtactttcc gaactggaca gcagcaggca gcagtattta ttagacagta
    15001 tacatgatat acagacgttg attacctgta cggggatcga tgattttata gaaaacaagt
    15061 ttcagttgaa caaagttttt catgttgcag acggtgttgt ttgcgaagga tgaaaagaaa
    15121 agacaggagg ttactatggg cacagagagc aatgcagaat acggtgcaga tcagattcag
    15181 atattggaag gtcttgaggc tgtacgaaag agaccgggta tgtatatcgg cagtacatcg
    15241 tccagaggtc ttcatcatct ggtttatgag attgtagata atgcagttga tgaagctctt
    15301 gcggggtatt gtgataccat ccaggtaacg ataaataaag ataattccgt aacagtcatt
    15361 gacaatggac gaggcattcc ggtcggaatc aaccacaaag caggtattcc ggcggtagaa
    15421 gtcgtgttta cgatcctgca tgccggcgga aaattcggcg gtggcggata taaagtttcc
    15481 ggtggtctgc atggtgtagg tgcgtctgtt gtaaatgcac tttctacaca tcttgaagtt
    15541 acgatttacc atgaaggtaa aatttatcgt cagcgatatg aaagaggaaa agtagtatat
    15601 aaattaaaag tgatcggaga atgtgatcca aagaaaacag gtacaacggt tacattcctg
    15661 ccggacagcg aaatttttga agaaacagtt tttgatttta atgtgcttaa acagagactt
    15721 cgtgagatgg catttcttac aaaaaatatc aagattgttt taagagatga ccgtccggaa
    15781 gaaccaatcg aaaagacctt ccattatgag ggcggtatca aggagtttgt gacttacctg
    15841 aataaaggca agacaccgct ttacccggat attatttact gtgaaggaat gaaggataat
    15901 atttatgtgg aagttgccat gcagcacaac gatggataca ccgaaggttc ctacagtttt
    15961 gtaaataaca tcacgactcc ggaaggcgga acccatctga ctggttttaa aaatgcactg
    16021 acaaagactt ttaattctta tgcgagactg aataaactga tcaaggacac cgagacactt
    16081 tccggtgatg atatccgtga aggtcttacc gtgatcgtaa gcatcaagat tgaagatccg
    16141 cagtttgaag gtcagaccaa gcagaaactg ggcaacagtg aggcacgagg tgccgttgat
    16201 aatattgttt ccgagcagct gatgatctat ctggaacagc atccgcaggt tgcaaaaatg
    16261 atccttgaga aatctatcat ggctcagaga gcgagagagg cagccagaaa agcaagagat
    16321 ctgaccagaa gaaaatctgc actggaagga atgtctcttc ctggaaaact ggcagactgt
    16381 tcagacaaag atccgaaaaa ctgtgaaatc tatattgttg agggagattc tgccggtggt
    16441 tctgcaaaga cagcgagaag ccgtgcaacc caggcaatcc tgccgcttcg cggaaaaatc
    16501 ctgaatgtgg agaaagcaag acttgaccgt atttatgcaa atgcggagat caaggcgatg
    16561 atcactgcgt tcggaaccgg tatccacgaa gatttcgata tttcaaaact gcgttatgac
    16621 aagatcatca ttatgaccga tgccgatgtt gatggtgcac atattagtac actgcttttg
    16681 acattcctgt accgtttcat gccggatctg atcaaagaag gacatgtata tctggcacag
    16741 ccaccactgt ataaattgga gaaaaataaa aaagtctggt atgcgtacag cgatgagcag
    16801 ctggcagcga tcctgggaga agtcggacga gaccagaaca acaagatcca gcgttacaaa
    16861 ggtcttggag agatggatgc agagcagctc tgggaaacta ccatggaccc ggaaaaacga
    16921 gtgctccgcc gtgttgttat ggataacgaa aattcttcag agctcgatgt tacctttaca
    16981 acgctgatgg gcgatcaggt agaaccaaga agagaattta ttgaagagaa tgctcagttt
    17041 gtaaaaaatc tggatattta gtgctcggaa gtaggaggaa aaactgttgg aagacaatat
    17101 ttttgataag atacatgacg tggatttgaa aaagaccatg gaagaatctt atatcgatta
    17161 tgcaatgagc gttattgcag cacgtgcatt gccggatgta agagacggtc tgaagccggt
    17221 gcagcgaaga gttttgtatt ccatgatcga actgaataac ggacctgaca agccgcaccg
    17281 taaatgtgca cgtattgtcg gtgataccat gggtaaatat catccacatg gagacagctc
    17341 catctacgga gcactggtaa atatggctca ggactggtca accagatatc cgctggtaga
    17401 cggccacgga aactttggtt ctgtcgacgg tgacggagct gctgccatgc gatatactga
    17461 ggcacgtctg agcaaaattt ccatgaaaat gcttgcagat atcgataaaa atacggtaga
    17521 tttttcaccg aacttcgatg aaacggagaa agagccagat gtacttccgg caagatttcc
    17581 gaatcttctg gtaaatggta cgaccggtat cgccgtaggt atggcaacga acattccgcc
    17641 gcataatctg cgtgaagttg taaaagccgt tgtgaagatc atcgacaacc gcattgaaga
    17701 aggcagagag acggagatcg aagaaatcct ggatatcgta aaaggtccgg atttcccgac
    17761 cggtgcaatg atcctcggaa caagaggaat ccaggaagct taccgcacag gacgcggcaa
    17821 gatccgtgtg cgtgccgtaa cagaaatcga atccatgcca aacggcaaga gccgtattct
    17881 ggtatcggag ctgccgtaca tggtaaacaa ggccagactg atcgaaaaaa tggcagaact
    17941 ggtacgtgat aaaaagattg acggaatcac agcgatcaca gatgaatcaa gccgtgaagg
    18001 tatgcgtatc aacattgagc ttcgaagaga tgtcaacgca aatgtcgtac tgaatcagct
    18061 tttcaaacat acacagctcc aggatacctt tggtgtgatt atgctcgcac tggtaaacaa
    18121 agagccgaaa gttctgaatc ttctggagat gctccagcat tatctgagac atcaggaaga
    18181 cgttgttaca agacgtacac agtacgaact caacaaagca gaagaacgtg cacatatctt
    18241 aaaaggtctg ctgatcgcac tggatcatat tgatgaagtc atcagcatta tccgtgcatc
    18301 cagaacagca gcacttgcaa aagaagcatt gatgacacgt tttgaacttt ccgatgcaca
    18361 ggcacaggcg atcgtagata tgcgtctgcg tgcactgacc ggtctggaaa gagagaagat
    18421 cgaagcagag tatgaagctc tgatggcaaa aatcaaggag ttgagagcca ttctggcaga
    18481 cgaaaatctt ctgcttggcg tgatcaaaac agaaatcatg gaaatcgcag ataagtatgg
    18541 tgatgagaga agaacttcca ttggatttga tatgtatgat atttctacag aagatctgat
    18601 cccgcgtgaa aatgttgtga tcgccatgac aaaacttggt tatattaaac gtatgagtgt
    18661 tgacaatttc aaagctcaga accgcggtgg caagggcatc aagggtatgc agaccatcga
    18721 agatgactac atcgaagaac tgctgatgat gaatacacat cactatctga tgttctttac
    18781 caacaccgga aaagtatacc gcctgaaagg atatgaaatc ccggaagcca gccgtacggc
    18841 aagaggaacg gcgatcgtca atctgctcca gctcgcaccg gaagaaaaga tcacggcgat
    18901 gatcccggtc aaagagtaca agcagggcaa atatctgttt atggcaacca gaaagggtat
    18961 tgtgaaaaag acaccgcttc tggattatgc aaacgtaaga aagacaggac ttgcagccat
    19021 cagcctgcgt gaagatgatg aactgatcga agtcaagctg accaacaaca agaaagatat
    19081 tttcctggta acaaaatacg gtcagtgtat tcgattcccg gaaaccgatg tccgcagcat
    19141 gggacgtacg gcgatgggag tcatcggcat gcagctcaat gacggcgatg aagtcattgg
    19201 tatgcagctc aatacacagg gagatcacct tctgatcgta tctgcaaacg gcatgggaaa
    19261 actgacctcg atcgatgagt tcaagagcca gaaccgcggt ggtaaaggtg tgaaatgtta
    19321 taagattatg gaaaagacag gaaacgtgat cggtgttaag aacctccata tggatgacga
    19381 gatcatgatg atcaataccg aaggcattgt cattcgtatg atgtgcagcg acatttccgt
    19441 tcttggacgt gtgacatccg gtgtgaaact tatgaatctt tcggagaacg tatctgtggc
    19501 aagtattgcg aaagtccgtg aaacttcagc agacagcgaa gatatcatca aaaaaatcga
    19561 agacgaaatg atcgaagatg agaaaaacga cgaagaaatc aaaaacgaag agacaacaca
    19621 ggagtaatca aactggagga agaccatgat tttgcgaaga atgcttgcaa tctggatttc
    19681 aaaagcaaca tgtgtagtat gtaaacttgc cggcaaacag ggggtaaccc tggccggcaa
    19741 aattgcactt aaaatagatc cgaaaatcct gatggaactt tccgggcagg tgcggaaaaa
    19801 gatttttatc acctgcggaa caaacggaaa gacgacaacc aataatctgc tctgctcggc
    19861 aatcgaggca gaaggtgaga aagtgatctg caaccacacc ggttccaata tgctcaacgg
    19921 tgtcgtatcg gcattcgtgc tggcagcgaa atggaacgga aaactggatg cggattatgc
    19981 gtgtattgag attgatgagg catcaacagt aagagttctg ccgtatttta aaccggacta
    20041 tatgattttg accaatcttt tccgggatca gctcgaccgt tacggggaga ttgatattac
    20101 gatggaactg ctcaaaaaag ccatgcatat ggctcctgat atgacacttg tcgtaaatgg
    20161 cgatgatgca ctttcggcat ttcttgcaaa agattccggt aacaaatatg ttacgtttgg
    20221 tatcggaaag cagtgtcagc aggaaaaaga aaatgcaaga gagatccgtg aaggtcagtt
    20281 ctgcaaatgc tgcggagaaa agctgaatta ccatttttac cattacagtc agcttgggga
    20341 ttatgagtgt ccgaactgcg gatttaaaag accgaagctc gattttgatg ctgaaaacgt
    20401 aaaaagcggt ggaaaacttt ccttcgatgt ggataacaga gaaataaaag ccaactaccg
    20461 tggattctac aacgtttata atattctggc agcctatgca gcagctcgca cagccggtct
    20521 ggaattaaag aattacaaca aagtgctggc agactacaat ccgcagaacg gacgtatgga
    20581 gtattttcac atcaaaaata ccgatgttct gctgaacctt gcaaagaatc cggctggatt
    20641 taaccagaat atttccgcag tgcaggaaga tgacaaaccg aaagatatca tcgtgctgat
    20701 caacgataaa gatcaggatg gtacggatat ttcctggctc tgggatgtgg attttgaccg
    20761 ttttcaggat atgaatgcag cgtcgatcac ggtcagcgga atccgatgcc aggatatgcg
    20821 tctgcgtctg aaatatgttg atattccatc caggctggaa ccggatgtag aaaaggcgat
    20881 ccgcagccgc attgcagacg gaaccggaaa tctgtacgtg ctggtaaatt atacggcact
    20941 ttatgacact cacaatatcc tgaaaaaatt agaacaggaa aaagcgtagg aggcggcaga
    21001 aactatgaaa attacaatcg gacaccttta tccggatctg ttgaacctct acggagaccg
    21061 gggaaatatc cagtgcatga tgaaacgctg ccagtggaga ggcattgaag cagaaacgct
    21121 ggaatttaaa ctggaagatg aagtagattt ttccaaactg gatattgtac tgctcggagg
    21181 cggttctgac cgtgagcaga tgatcgtctg caaaagactg cagcagatcc agaaaaactt
    21241 taaagattat gtggaagacg gcggtgttgt gatcgctgtc tgcggtggtt atcagctgct
    21301 tggagattac tatcagacgg acgaaggccg catggaagga ctgcatttgg tcaaactcca
    21361 cacggagcag aaaccgggaa gactgatcag caatattgta ctgaaaagtg atctctttga
    21421 tatgccggtt gtcgggtttg agaatcatgg cggacgtacc gatatcggtg ataaccagcc
    21481 gtttggcaaa gtcctgtatg gttccggaaa cgacggtgaa tccggctatg aaggtattgt
    21541 atacaagcat gtgatcggca cctatctgca cggaccactg ctgccgaaaa atccacaggt
    21601 ctgtgactgg ttgataaaga aagcactgga gcgaaaatat ggaaaagaca ttgagcttca
    21661 accactggat gatcaggaag aaaaagaagc aaacgcttat atagtaaaca gatttgtaaa
    21721 atagagtatc aaaaaataac acacaaaaga ataaatttta tgattttctg atacaaaaca
    21781 gaaaattaca gggcaaaata gatagaaaaa aagaatctct gaaagctgat tattggaagg
    21841 atggataaaa aatattccac taatcagctt tttttagtaa aaaatagttg aaaaagagta
    21901 ctgaaaatgg taacatgaaa tagatatagc taattttttt gtataagcaa ataaggaggg
    21961 cttttatgaa atggaaacag tatttagctg tcatgacagc agctgcaatg gttatttccg
    22021 gacctgcagt accgatgagt caggtattcg cagcagatgc agtggcagca gaaggacagg
    22081 cagatgagga aacagtatat gagttgccgg cagatagtag tacactgacg gtagaggcag
    22141 agaattttac catgcatccg ctgaatggcg atactaaaaa tcatgtacat gtagaagatg
    22201 ccagctgggc aagtggtggc aagatcgcaa actggtttga aaatggagat gctatttcca
    22261 tgaagttcaa tgcaccggaa gcaggtgagt acagcgtaac agctacttac cgcagcggac
    22321 gtacagaggg tggtacacca aatgcatttg tatgggaagg caccaacatt gagagcggaa
    22381 gcgtagatgt ttatggagaa caaaatgcaa ctaccacaca tacggtaact tttacagtta
    22441 aagttacaaa agccggacag ggtgaactga gatttactgc tacaactcca aaagttggac
    22501 cgcaggtaga taaatttgaa tttaagaaaa aagcaaacgt agctgtatca gacgttacac
    22561 ttgataaaga aacagctgtt ttgaataaaa aaggtcagac agtacagctg actgcaaccg
    22621 tagcaccgac agatgccagc aataaaaatg taagctttgc atcaagtgat acaaaagtgg
    22681 caacggttga tgcaaaaaca ggaaaagtaa cagcagttgc aaatggaatt gcgaatatta
    22741 cagttacaac agaagacgga agcaagacag cagtctgcac cgttaccgta gatatcaaag
    22801 atactacaga gacagaagat gcagaggcac caaaaacctg gggagcaaca ccaaatgatg
    22861 aacagctctg gtatatgaaa cagggaacag ccgcattctg tcactttgga ccgaatactt
    22921 ttaacaacgt tgaatggggc gaaaattacg gaacaaagag tccggcagaa atcttcacac
    22981 tttccgacga ttttgatgcg gaagaactgg taaaaacagt aaaagaagct ggattcagcc
    23041 gactgatgct tacagcaaaa catcacgatg gattctgtct gtgggcaagt gattatacag
    23101 actatgatat cgcaagcacc aactacaagg gtggtaaggg cgatatcctg gaagagattt
    23161 ccgatgcttg tacaaagtac aacctggata tgggacttta cctttctccg tgggatatcc
    23221 atgaagataa atacggatgc tttggtgata acaacaacac caagagcaac gaaggaaaag
    23281 gtgagtttac agactataac aaactgtata cgacctggat cagagaaatc tgtactgcaa
    23341 agaaagcaga cggaagctat aaatatggta acaataaccc gaatcgtcgt tccgaccgtt
    23401 ttgtagaatg gtggatggac ggtgcacagg gaagcggaag ccatgtacag acttacgact
    23461 ggactgctat cttcagtgca atccgtgata caaacccgag ttgccagatt tttggtacac
    23521 atgcagcagt aaaaggtatc aatgcttctg ataaagcact ggcaagcacc ggtggtatcc
    23581 actggatcgg taacgaaaat ggaattgcag ccgatgagac atgggcaaaa gtagatgccg
    23641 gaacaagtta cgaaaataca acgctgtatc ctagagtaga cggagctatc gtaggaaaag
    23701 cagatggaga tcagtggtct gtaccggaag tagataccaa gatgctggct ggatggttct
    23761 ggagagatgc agcaggtgat aacacttcaa aatctgaaaa agaactggct gatatttatt
    23821 tccgtaccgt aggtcatggt gcagcactgc ttctgaacct gtctccgaat aaaaagggaa
    23881 ccatcggaac agaacagctg aaccgtttca aagaatttgg aaacaatatc aaagaaactt
    23941 tcaaaaatga tctgacaaaa gtaagcggag taacagctac tgcagattct acctggaaag
    24001 attccgataa atacggagca gcaaatgttc tggataccat tccaaacgga gaaacatatg
    24061 acaatacata ctgggcaccg gcagaaggaa agacaacagg aagtctggaa atcaaccttg
    24121 gcggtgtgaa gaaatttgat gtggtatcca tcgaagaata tatccagaaa ggacagacca
    24181 tttcttcctt tactgtagag tataaggata tcacaggaag atggcatgat ttcgcaagcg
    24241 gaaagaccat ttcttccaaa cgtctgtgcc gtggtgaaac agtagaaggt actgctgtac
    24301 gcatcaacat taccggtgca aaagatacac cgaagatctg caacgtaggt gtatttaagg
    24361 cagcaaaagg atttgaagta gcttcttcag gaagcgatgc aagcctgccg agcaatctga
    24421 aaaaagtttc tatcaatgat gcgacaagag aaggaacttg gaattttgaa gcagatgaag
    24481 acggaaatgc aaaaagttct gcatggggtg atacagcagg gctggcagca acctttacct
    24541 ttacaggaac aaaagcatgg gtagttggta cagcagatcc gaatcatggt atgatggatg
    24601 tttatattga cggagaaaag gtggacagcg taaatacaca gcagtcaccg agaaagatgg
    24661 gagcaatcct gtatacaact ccggatcttg aatatggcac acatacgatt aaactggcac
    24721 gtgttacaaa agctcttggt atcagtaaaa tctggtatgc agatggttct ggtatcttta
    24781 cgatgaaaca gtccgagtac gatctgatgt atggcggaac aaccgaagta gagatcacac
    24841 gtacaggtgg ctctaagggt gaagttaccg taacttactc aacacagtcc gcaggagctg
    24901 agcagggtgt aaactataca gaccttgcag gaactgttac attcaaagat ggtgaaacat
    24961 ctaagaagat cactctgaca ggtcttgaga atgaaaatcc ggacagaatg attgacggaa
    25021 aagatttcta tttcacactg atggcatctg acaatggttc cattggaaat gacagttatg
    25081 cacatgtagt cctgtataat gcagacgtaa caaaagttgc agagagaatc gaagcaatgg
    25141 atcttacagg ctatacagca gaaagtgtta aagtattaaa tgatgcaatg agcgaaatga
    25201 aagcacttgg aacaactgca acaaaagatc aggtcaaagc tgcagttaaa aaagttctgg
    25261 atgcaaagaa tgcactgaga gcagcagatg aaaataatcc ggcatatgaa gcagtaacag
    25321 gagtgagcct tgataaaaca actgcaaaac tgacagaaaa aggtcagaca gtagaactga
    25381 aggcaacggt agcaccggca actgcaagca ttaaagctgt aaactttgca acaagtaatg
    25441 cagccgtagc aaccgtagat gcaaacggaa aagtaacagc agtagcaaat ggaacagcaa
    25501 cgattactgt aacaacggaa gacggaaaca agacagcaac ctgcactgtt actgtagaaa
    25561 ttccgaagac accagatccg acaccgacgc cagtaccggc agatttggtt gagaaagtaa
    25621 ataatgaaat tgtagctgct gctaataaaa aagaaaatga ttacacagca gattcctgga
    25681 ttgcatataa gaaagctctg gaagcagcaa caaaagtagc aaaagatgaa aaggcaacga
    25741 aagcagactt agagaaggca cttgcagatc tgcagacagc agcagctaaa cttcagaaaa
    25801 aagcacctga gccggaaact ccgtcaaccg attccaaaaa tccaactgtt ggaacagaag
    25861 caaaggtagg aaaaggtatc tatcgtgtaa caagcgcaaa gaaaaagaca gtagttctgg
    25921 taaaaccgcg taaagccaac aatacaagct ttaacgtacc aaagagcgta aaactggcaa
    25981 acggaagata caaagtcgtt ggaattgaaa agaatgcatt taagaacaac agaaaactga
    26041 agaaagttgt catcggaagt caggtaacaa agatcggtgc aaatgcattc agcggagcta
    26101 aaaatctgaa gactattaca atcaaatcca aatctctgaa gagcgttgga aaaaatgcat
    26161 ttaagggtat ccataagaac tgcaagatta aagttccagc taagaaatta aacagctaca
    26221 agaaacttct gagtaaaaaa ggtcagaaag caagcgtaaa aattacaaaa taaaccagag
    26281 ttaagaacat tgattctgaa ataaaaagcg gttaaaaaga gctgccacaa aggtgataaa
    26341 acaccggagt ggcagttctt taatatgagc aaaaaggtta tgtatttatg ttttctggaa
    26401 attccgctat ttcgcatgcc tctttttctg aaaagccttt ttcaatgaag taaaaataat
    26461 ttgtaaactg tgagctttaa aaagcaaaaa aagtatatga gctttaaaaa gcaaaaaaag
    26521 taaggtataa tataaaagaa atataataaa atcggaggag aaagagtgaa aaaatttgct
    26581 tatcaggcaa cgcagagatt gtatgcactt atagttggaa taatattttt gttgatatta
    26641 tttggacaat ggtgcagctt taaattaaaa aaaggatttt tgatccccaa tattgtaatt
    26701 ttagggggaa ttattttagc tggggcattt gtatattact ggcgatataa aagaaatgga
    26761 aagcagaagg cagaaaggat atggaagtat gatttaattg taagaatttc tttgattata
    26821 ttatttgttg ttcaaatttt tattgcttac aatatagttt ttgagcctgg ctgggatgca
    26881 ggaggaattt ataacagtgc aaaaatattt gtaaacggaa atcgggcgga tatcgttata
    26941 agatatccat tttctatgta tccaaataat ttactgctgc
//
LOCUS       DAWDWH010000007        26923 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_1078_length_26923_cov_12.948452, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000007 DAWDWH010000000
VERSION     DAWDWH010000007.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 26923)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 26923)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..26923
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1078_length_26923_cov_12.948452"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    572..581
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19289..19298
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20736..20745
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    23116..23125
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    25762..25771
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6089 a         7247 c         5273 g         8264 t
ORIGIN      
        1 aagatttgga aaatttatgg atgtattcat aacatcacct ctttattttt ccaattatac
       61 attgaagcgg aacaaaacaa catctccgtc tttgactacg taatctttgc cttccaatcc
      121 taccagtcct ttttcttttg ctgcagaata gcttccgcaa tccagaagat cctgataatt
      181 gactacctcg gcacggatga atccacgttc aaaatcggaa tggatcttgc ctgccgcctg
      241 cggtgccttt gttccttttt tgatcgtcca ggctcttgtc tcagtctcac ctgcagtcag
      301 gtaactgatc aggcctaaca gactgtagct tgctttgatc agtttctcca gtccggactc
      361 ttttaatccc agatcttcga ggaacatttt tctttcatcc tcatccagtt cagaaatctc
      421 ctgctcgatc tgtgcacaga tcacaaatac ttcactgccg ttcttagctg caaaatcccg
      481 gactgcctgc acataagcgt tggatgctcc atcatctgcc agatcatctt cagatacgtt
      541 tgccgcatag atcaccggtt ttgcagtcag annnnnnnnn nttcctcatc ctcatcctga
      601 agttcaaatc cgatcgccag attcccttct tccagatatg cttttaatct ctgcaggaaa
      661 tccacttctt ttgcaagctt cttatcattt cttgcacctc tgctgctctt tgcgatcctt
      721 ctctccagga tctcgatatc agaaaagatc agctccaggt tgatcgtatc aatatctctt
      781 gtcggatcta cgcttccgtc tacatggatc acattggtat cctcaaagca gcggacaaca
      841 tgcacgatcg catcaacctc acggatattt gccaggaact gatttccaag tccctctcct
      901 ttggatgctc cttttaccag acctgcgata tctacaaact cgatcacagc cggtgtcact
      961 tttgctgagt gatacagatc tgctaaaaga ttcagccttt catccggaac ggttacgata
     1021 ccgacattcg ggtcgatcgt acagaacgga tagtttgcgg actctgctcc cgcctttgtt
     1081 aaagaattaa acagtgtact ttttcctacg tttggcaatc ctacaatacc aagtttcatt
     1141 tgtaatttcc tctctttatg taaatgacat tttgctcttt atcagcacca acagtgtaac
     1201 acagtaaacc tcctgattgc aagtccccag aaaaagaact catcgacaaa attttactgt
     1261 ttttcttcgt tattcgacct ttttcgacag gaatatcagt ctttcgactc gatcaccaac
     1321 agaacccctt ccgaaaagtt gatccgacat gcctcttctg tgatctcatt gcttacgcag
     1381 agcggatcca ttccctccag aagataatga tcaagcggat aaaaaacacc ctgcagcgtc
     1441 agatctttca ctgcacctcc catggcaaac aaagaaagat atttgccgaa ctgttcctct
     1501 ttttttatgg atactgcatg atccgtcagc cacattttgt tgtgttcatc cagaagatat
     1561 gcccgcacac ctttttgcag agcaagaaac aggatccgta catttgcagc ggtatggtcg
     1621 atgcgtgccc cggttccgcc aaggatcgta atttcttctg ccccaagttc cagtgccaga
     1681 cgaagagcca catcggtgtc tgtataatct ttgaccggat tgagcctttt gatcggaacg
     1741 tcggtatttt tctgaaaata ctcgtattcc gtctcgccag ccgaatcaaa gtctccgaca
     1801 atgtgtgtgg gcatgatttg atttttgtga cagaagatca ctcccttatc tgccccgatc
     1861 acgtaatccc aggtctgtgt cctcaaaaaa gcaagggcaa aatcggtctg aattttgccc
     1921 ccgcttatga tcactgctcg cttcatttgt gattctcctg ataatctgcc agcagtttcc
     1981 ggtatttttc tgcatttttt gccgtttctt tgccaaaaac agccgatccg gctaccagga
     2041 tgttggctcc tgcctctaaa acagcatcga tcgtcgcagc gttgatgccg ccgtcaactt
     2101 caatatcggt ctgaagtccc cgctcttcga tcatggtacg cagctctttc acctttccgg
     2161 tgcagtacgg aatgtatttc tggtttccaa atccaggatt tacggtcatc agaagtacca
     2221 tgtccacttt ctccagaagg taatccagct catgcagaga agtagaaggg ttcagtgcaa
     2281 taccagcttt ctttcccagc tgatggatct gctcgattgc acgatccgga tgtttgcagg
     2341 cctctgcatg gatcgtgatg atatccgccc cgcttgccgc tgtctcttcg atataacgct
     2401 ccggctgttc taccatcaga tggacgtcaa ataccatatt gctcattttc cggatggatt
     2461 tgatgatcgg gaatccaaat gaaatgctcg gtacaaacat accgtccatc acatcaatat
     2521 gaagatagtt tacgcccgtc tgctcaactg cctttatttc ctctcccagt cttgcaaaat
     2581 ctgcggaaag aatagacggt gataattcgt acttcataaa aattagtacc tccttttttg
     2641 tttcagttct tcatacagtt ctttatagct ttcgtagcgt atagaactga tctttccttc
     2701 tgccagtgct tcttttacac cacagtcagg ttcactcaaa tgcatacagc cctgaaaacg
     2761 acagtatggc tcatattttc gaaactctgc gaaatattct tttaaatctt ctttttcaaa
     2821 atcggtaaga tacaacgaac tgaatccggg cgtatccatc agatacgtgt tgtcaccgat
     2881 ggagaacagt tcagaatgac gcgttgtatg cttgccgcgg tcgatcttct gactgatctc
     2941 accgaccatc atctctgcct tcggatagag aaagttgatc atcgatgact tgccgacacc
     3001 ggacgggccg gcaacaacgg tcgttttatc tttcaaaagc tctcgtatct gttctgtccc
     3061 tgcctgctct tttacactgg taaaaagaac ctgacagcca cagttttcat aaatctcctg
     3121 aagtcttgcc gcctcttgcg gctctgcaat atcacttttg ttaaaacaga caacgactgg
     3181 tacattctga cgctccattg agatcaggaa acgatccaga agactcagat tcggagccgg
     3241 cctgctgatt gcaaaaatga ccatcgcctg atctacattt gcaacggccg gacggatcag
     3301 tgcatttttc ctggcaagga tatcccggat atttccttct cttttttctt cgtccaggat
     3361 atcgatcacc acatcatctc cgaccagcgg ttttatttta ttttttcgaa aaataccctt
     3421 tgctttacac tcatacacgc cttttccggc gatatggaca taataaaacc cggcaattcc
     3481 ttttataatc tttccctgca tgaaaactcc gtattctgca caccgctatt tgctgaacgg
     3541 tacgccttca tattctactt ttttcaatat atttccatcg tcatccagca cataaacata
     3601 cgcagttgca ctgctgccgg aatcggatgt cattttcagg ctgtacggga actcaaccac
     3661 ttctccgtcc accagagtcg ttgtcttgcc gtcctgcacc agctcaatgc ggactttctc
     3721 atcgttgtag tcctgcggtg cataaagctt tacatcggta gaatattctg cctttgcact
     3781 ggtttctttg gattccgttt cctttttcgt ctctttgacg gtctcctgtg tttccgtatc
     3841 cgtttccgtg tccgatgcag gatcggaact gtcgacactt ccgtctccgc cattgctgat
     3901 gtaaagtgta acggtagaat tcggggcaac ttcctgtcct gctcccggac ttgtataact
     3961 gacatgtccc tgggcgatgg aaatatcata cacttcctgc tggtcacatt tcagccccag
     4021 cttttccagt gctgctttcg catcgtctgc tgactcattc tccagatccg gcagggtgat
     4081 cttgtcgctt ccggtactta ccgtgtaaga aatggtcgta tttttatcaa ccatctcgcc
     4141 ctttgccggc tgctgagcca tgatctgacc cttgtcataa tcggtggacg gttcttctcc
     4201 ggcatactca agcccaagac cagtatctgc cagcatttct tttgcctctg cttctgtttt
     4261 gccaaggatg ctcggcactt cgacctgacc ggagatctgt gtcgtctcag ttgcggtcgt
     4321 tgtctctgtc tgcttcttgt tgcttccgcc tgccaaaaat ccaaggtcaa acaatccggt
     4381 cgcacttccg accagtgcca gaaagattgc caggatcaga acgcctacga caatgctgcc
     4441 gatcgtcatg aatttctcca gtttcggatt gacttccggc tcctgcggct cttccggctg
     4501 ataatatccg ccggcataat tctgtggatt ctgatatcca ttgtttgcat actgctgtcc
     4561 tctgccctgc atattctgcg gatttcccat attctgcggg ttctgaggat ttggcatgcc
     4621 ggtattgacc ggttcttgat ttgcaggata aggctcttct gcctgctgca tctcattgcg
     4681 tccttctttg atcttgttca gctcatcctg agagacgatc acggtatgtg cacggctgtc
     4741 aagcggtgca atctttacaa agtcaccttc cgggttgacc agagactcct ttaagtcact
     4801 gatcagctct tccatgtcac tgtatcgtcg atccgggctt ttctgcgtac atttatagat
     4861 gatctgtacc gtacttttcg gcagttccgg cacatattta cgcggtgact ggatctcctc
     4921 ctgcagatgt ttcagtgcaa tggcgaccgt cgtgtcaccg tcgaacggta catgaccggt
     4981 cagcatctca tacatcgtga taccaagcga atagatgtca ctcttgtagt cactgtaacc
     5041 gcctcttgcc tgctccggtg aactgtagtg taccgagccc ataacattgg aactgatcgt
     5101 attggatgtc gccactcttg cgataccaaa atccgtgact tttacttttc catctgtgga
     5161 gatgatgata ttctgcggtt tgacatcacg atggacgatg ccgttgttgt gtgccgcctg
     5221 cagccccatc gatacctgga tcgcaatgct ggtcgcttcc cgcactgcga gtcttccctt
     5281 tttctcgata tattctttta aggtgatccc ttctaccagc tccatgacaa tgaaactgat
     5341 gccgttgtcc tcacctacat catagacatt tacaatattc ggatgtgcca gtcctgccgc
     5401 agactgtgcc tccacacgga atttcgtgat aaatccctta tcttttcgga attcaggttt
     5461 caatactttc actgcaacaa agcggttcag tgtatggtct tttgccttat atacatctgc
     5521 cataccgccg gaaccgatct tgctgatgat ctcgtatcga tcctggataa acatgccttc
     5581 ttttaacatt gctccacctc acctgtcagc ggctgtgcca gtacaaccgc gatattgtcc
     5641 ttgccgccat tgcgatttgc ccgttcaatc aggtccatgg cgatcttcgg cagctctctg
     5701 ccgcttttga ttattttttc aatttcttca tcttctacca tgttgctcag tccgtcggaa
     5761 cataagagca ggatatctcc ttttcttaag tcctcgtcaa aaaagtcaat ctccagtttt
     5821 tgtcctgcac cgactgctct ggtgatgata ttcttgttcg ggtgatttct cgcctgctcc
     5881 gggcttaact cgcccgcccg caccatctct cctaccagag aatggtctct ggtgatctgt
     5941 ctgatgtgat cccgtatcag atacagtctg ctgtctccta catttgcaac ataaaggtaa
     6001 ttttcaacca cggttgcggc tacgacggtc gtacccatac cgctcagttc ttcgtcttct
     6061 gctgcctttt ccagcactct ttcgtttgct gtttcaattg cacttcgaag tacacggatc
     6121 ggatttcgtt ctctgcttct tttaatatga ctgacgatca cttcggccgc atagcgggat
     6181 gccatatcgc ctgcctgatg tcctcccata ccatccgcaa cgatcagaag gttcggcaat
     6241 ggtcctaccg gagtgtcaga agcaaacaca aaatcctggt tcgtggttct tttctgccct
     6301 acatctgtaa cacagcatga tctcatgcct ttactccttt ttttctttat ccatataact
     6361 tttcctaagt tgtccacaag caccgccaat atcacgtccc atttctcttc taatagtaac
     6421 atttattccg caattttcaa gtttattttt gaactccaca atcacttttc gttccgactg
     6481 cacataagaa cgttctttta ccggattgac cggaatcaga ttcacatggc agttgatgcc
     6541 atggatcagt tcgtaaagct gctttgcatc ttcttttgaa tcattctgtc cgccaacaag
     6601 gctgtactca aacgtgatgc gacgtccggt cttttcataa taataccggc aggcatccat
     6661 gacctccccc agggaatagg tgtaggcaat ggatctcata ttttccggcg atcggaagca
     6721 gtgcttttcg tttttcctga ttcggtgcgt ggagcgaaag tgccagtgtg atctgaaatc
     6781 cttcatctgc aagctgacgc atccgcggca cgatcccgca ggttgataag gtaatgtttc
     6841 gctggctgat attcagtccg tgttcatcac tcagcatccg gatgaatttt accgcattgt
     6901 cgtaattatc cagtggctct ccggtaccca tcatcaccag gttggatacc cgctccccga
     6961 tgtctttctg gatacggtag atctgatcga gcatctcccc cgccgtcaga tctcttgtct
     7021 ttcctccgat cgtggaggca cagaaacggc atcccatgcg gcagccgacc tgcgtggaaa
     7081 tgcacaccga attgccgtgc tggtaacgca tcagcacact ctcgaccaca tagccgtccg
     7141 aaaggcggaa cagatatttt ctcgttccgt ccagttcgga aaccagcgtc tgtacgttct
     7201 caaggcaggt atattcacag gtttctgcca gtttttcccg cagatttttt gagagattgg
     7261 tcatctcttc aaaaccatcc gcctgttttt catgcatcca ctggtagacc tgctttgccc
     7321 ggaacggctt ttcgccgatg ctgacaaaaa aatctttcag ttcgtcataa tccaatgatt
     7381 tgatatctgt tttttccatg ccacactcct actttacgcg tttcaggcgt gcaatgaaaa
     7441 atccgtctga tttatgtatg ccgggcagaa gctgaagata tccggcactg gttgtcttac
     7501 ccttcagctc gtcgcagatg tacggatcaa tgctttccag cacaaacgga taattttcca
     7561 gaaaccattt gacgttgtac tgattttctt ctgccccgat cgtacaggta ctgtaaatca
     7621 atgttccgcc cactttgaca tacttccaga ccgtatcgag gatcttacgc tgcagtttga
     7681 tgatctccgt ctgttttgcc ggtgtcatct tgtagcggat gtccgccttt ttgccgatca
     7741 cgccaagtcc ggagcaaggc acatctgcca gcagaatatc cgcacgctcc acggattccg
     7801 gatcaaaaac ggtcgcatcc tgcagggtcg cagacatatt gatcacgccg atacgctcaa
     7861 tattttcctg catcagctgg actttgtagt cggtgatatc tcttgcctcc acatgaccgc
     7921 ttccgtgcag catatctgca aggtgaaggc ttttgcctcc cggtgcggca cagacatcaa
     7981 tgcagtagtc gccccatttc ggtgctgcaa tctcagaaac cagcatggag ctgacatcct
     8041 gcacttggaa ccagccgcgt ttaaaggcaa gcaccgcttt catgtaatta tagccgcgga
     8101 tgccgagtgc atagtccaga tacggactct gctctacctg gatcccctgc tctttgaggc
     8161 tctcgataat ctgctctttt tctgttttat ccagattgca gcggatcgaa gtcggttttt
     8221 cttcatgcat atcctggcag atcttctcca ctgcatcaaa gccgtagagt tcacaccagc
     8281 tctttacgat ccattccggc atggaatagg tgactgaaag ataaagcaac ggctgttttt
     8341 cttttgaagg atattccact ttgtccagat ttcttgcaac acttcgaaga acaccgttca
     8401 caaaaccttt gagctgataa aagccccgtt tctgtgccag acgcacgcct tcgttacaga
     8461 cggcagaatc cggtacactg tccatatatt ttaactgata aaccgacata cgcaggatgt
     8521 tgcggatcac cggtttcata ttttccactt ttacttttga aaaacgctca atgatataat
     8581 ccatctggag catattttcc agcgtgccct cggtcacacg cgtaataaat gcccggtcac
     8641 gtttgtccag gtactgatat ttttccagca catccttgag cacgatatgg ctgtactgtt
     8701 cttcttccac gatatccatc agtgcaccga ggataatttc tctaagatta atcatctctt
     8761 ctgtttcctc ctgctaaaat gacaagacgc agcagctgca gtatggatga tgcaagactt
     8821 gctacataag tcatagctgc cgctctgagc actttttttg ctcctgtgat ctcactctct
     8881 ccaagcattc cgtttccctc aaggatcctg atcgcacggc tggatgcgtt gaactcaacc
     8941 ggcagtgtca caagctgaaa gatcacagcc agtccaaaca gaagaatacc gagcatcaga
     9001 agcggacgca tgctgaaaat cagccctgca acaaaaatcg gccaggaaag actggaaccg
     9061 atgttggctg ccggaaccag tgtgcttcga agtaccagag gtccatatcc tttctgatgc
     9121 tggattgcat gaccacactc atgtgctgcc acgcatactg ccgcaacgga attggagcca
     9181 taggtgctgt ctgaaagacg cagcactttt gccctcggat cataatgatc agtcagctct
     9241 cctgccacac gctcaatgcg gacatcatag ataccgctga tgcggagcac ttcctgtgcc
     9301 gcctgtgcac cggtatagcc tgccatacag cgaacgcgtg aataccttga aaaggtgctt
     9361 ttcacgccag ccgatgcgat cagcgtcagg atcagccccg caaaaacaag tataaaagtc
     9421 ggatcataat accatccata cattataaaa tcatcccttt ctcaagatga cagccgcgaa
     9481 ggaaggctgc cgtgtccata cgtttcttac cttctaactg aagttctttc acggacagga
     9541 tcttttctcc tgtctggatc aggaactcgt ctttttctgc tttcagcacc gttccgggct
     9601 gtgctttttc accggtctgc ccacatacat ctgctgccca gattttcagg gtcttaccgt
     9661 ttagtttcgt ataggcactc ggccacggat tcagtccgcg gatcagacgt tccagctctt
     9721 tcgccgggcg gttccagtcg atcgctccca tatctttggt cagcattttt gcataagcgg
     9781 tcggcgactc accctgtttc tgcggggtaa ccgtgccatt ttccagtgca tcaagcgtct
     9841 caagaagcag atctgcccct gctttgctca gtttgtcaaa cagacttcct cccgtttctt
     9901 tttcatcgag cggcacgacg gttttcagca gcatatcacc ggtatccagt ccggcatcca
     9961 tctgcatggt tgtcacgccg gattcctttt caccgtcgat caccgcccac tggatcggag
    10021 cagctccgcg atatttcggt aaaagtgaac catgcacatt gatgcagcca tacggtgcga
    10081 tgtccaggat ctcctgtggt atgatctgtc caaacgccac aacaaccagt acatccggtg
    10141 caagttcccg gatcttttgg atcacttccg gatctcttac ctttttcggc tgatagaccg
    10201 gaatattctc ccggagtgcc acttctttga ccggcgtaaa ctgcatcgct tttccgcgtc
    10261 cctttggctt atccggctgt gtaaagaccg cggtcacttc atgtctggat gaagccaggg
    10321 cttccagtgc ctcaaccgca aaatccggtg ttcccatata tatgatcttc atcttatcgc
    10381 ctcctgtttc ttttttacta tattttcatc actcttcttc atcataagga gcgacatctt
    10441 ctaattcgcc ctctactttt tctacataca tatgcccatc cagatgttcg cattcgtgac
    10501 agatggcacg ggccagaagt ccttcgccct ccagaataaa ttcattcata tcttcatcat
    10561 atgcttttac tttcgcataa tcaggccttg taacgacccc cgtttttccc ggaacactta
    10621 agcatccctc ataacctctc tgctctccgg ctgtctcgat gatcaccgga ttgatcaata
    10681 cgtgtggatc atccccgcag tcgataacta cgattctttt cagcatgccg acctggtttg
    10741 ctgcaagtcc gacgcccatg gcatcgtaca tcgtctcaaa catatcatcg atcagttctt
    10801 ttgtttttgg cgttacttct tttacttctt tacatacttt cgtcagtaca tcgtctccga
    10861 ttgtccttat tgttctggtt gccatctctt ttatcctccg ctttctctaa tctctgtcat
    10921 actgaatggt aattttatca aaacctctgt ttatttcaat atattgttct gtccgctctt
    10981 tgatctgatc cagcacttct tttcgcccgt gccttacata gatcactttt ctgtaaatat
    11041 ccgcaacttt cgaaacgctt tcatctgccg gtccgatcac tcttgcctgt gttttcttcg
    11101 ccagtacatc caggtatttt ttcaaatatt ccatcgccat ctgaaggtaa ctttcatcct
    11161 ccccctgccc gtgcagggca agcataccgc cgaccggcgg ataatccatc aggctgcggt
    11221 acaggatctc ttcttcataa aaagcctgat aatcctgagc tgccgccgct tttatactgt
    11281 aatgatccgg ctcataagtc tggatcacga cacttcccgt tttttcacct ctgccggcac
    11341 gtccggccgc ctgcgtcagc agctgaaagg tccgctcggc tgcccggtaa tcgctcatgt
    11401 ggagcgacag atccgctgcc agtgccccga ccagcgtgac atctgcaaaa tcatgccctt
    11461 tgacaatcat ctgcgtcccg accagaatat cggctccgcc ttttgcaaag gtctctaaaa
    11521 ttttatgatg tccgtctttc cctctggtcg tatcggcatc catacgcaaa atacgtgcct
    11581 tcggaaacat acgctgcacc tggcttacta cctgctgtgt ccccacctga aaacttccga
    11641 taaaaggaga accgcaggac ggacagaggc gaggatttgg ctgtgtatag ccgcagtaat
    11701 gacacaccag cttttcgcct gccgtacctt ttttgtgaag tgacagggac acgttgcagt
    11761 gcggacaccc gatcaccgta ccgcaggaac ggcatgccac aaaaccggca taaccgcgtc
    11821 ggtttaaaaa cagcatcgcc tgatcgcccc tctcgagaca catcccaagc tgctcttgca
    11881 gatatcggct taaaatactg cggttgccct cccgcatctc gtttcgcata tctccaatcc
    11941 agacatacgg aagcttttgt ctgttcgccc gcctcttcat ctccagcagg caatattctc
    12001 ccttttctgc ccggtaatac gactccaccg acggggttgc cgatcccaga atgagcttcg
    12061 caccttccat ttcggcccgt ctctgtgctg tttctcttgc atgataacga ggaaccgtct
    12121 cacttttgta ggactcttcg tgttcctcat cgataataat aatgccaaga tttgaaaatg
    12181 gtgtgaacag tgccgacctt ggcccgatca taatgtcaat ttcaccattt tttgcttttt
    12241 cgtactgatc atagcgttct ccggctgaca tccgtgaatg cagaatggat acacgacttc
    12301 caaaccgctg gtaaaaccgc aacaccatct gatacgtcag agcgatctcc ggaatcaaaa
    12361 agatcgcctg tttgccctgt tttcgcacaa aatcgatcag ttccatgtag acttctgttt
    12421 ttccgctccc agtcacacca tgcaacagat aaacagactt atctttctga tcccactctt
    12481 ttaaaatcgt ttggactgcc agctgctgtt cttcgttcag aaaaaagtct ctgcctgtct
    12541 gcatttttac ctcttcctgc ttttgatcgg accgggagat catcagatcc agcggttcgc
    12601 ggtagatccg catgctttcc agctttacga tacccttttc ttccattgcc cgtacaactg
    12661 gcatggtaac gccatactgc tcctttgccc gctcccagga aagaactgcc atgccttttt
    12721 cgttttgctt cgcaatcacc agcaaagcat ctaaaagtct tgccctggct ttctgatttt
    12781 tcttttccag aacctgcagc atttcccgtg cttctttttc cggcagcagc agacgaatct
    12841 gtttctgttc tttctgtgcc actttctgtt tgaccggaag cactgctttt aatgcctgga
    12901 tcatcgtgga accataataa tcccgcatcc acgcagccag tgcgatcagc tttccttcta
    12961 cgcctgtctg acctgcacag actccctgta tttctttgat cttttccggc ggataatcgg
    13021 tccggtccgt cagtttgacc acatagccct gtatcctgcg gttgccgttt ccaaatggaa
    13081 ccgtaacggc tgttcccgga tgaatctgca tccgcagaac atccggcacg cggtactgaa
    13141 aaatcttatc cagcttttcg tggctgatat ctacgatcac atttgcgtaa agtacttgtt
    13201 ctgttcctga cacttacttt cctttcgctg catcttccgc aagaatctcc cggatcagca
    13261 cacgctcatc catcggatat tttttgcctt cgatctcctg gtaatcttca tgtccttttc
    13321 cggcaagaac gatgacatca cccttctgac cgtggtggat tgcccaggcg atcgcctctt
    13381 tccgatccgg tatctcgatg tatttaccat ccgttttttc gatgccggtc cggatgtccg
    13441 caataatgtc cagtggtttc tcgaaacgtg ggttatcgga tgtgatgatc gtcagatccg
    13501 caagtcttcc ggagacttct cccatctcaa atcttctgga tcttgcacgg tttccgccgc
    13561 acccgaacag acataccagt ctctttggat gatattcttt taatgtcgtc aaaaggctct
    13621 caaggctcat ggcattatgg gcgtagtcga tcatcagcgt aaaatcatcg gaaactttga
    13681 tcatctcgat acggcctttt actttcgcct gtgccagtgc ctttaagatg tcctcttttg
    13741 agacaccgaa atgacggcag actgcgatcg ctgtcagaga gttgtagacg ctgaatttac
    13801 ccgggatatc aatctcgaca tccagatgga aatcctttgc ttccgccgtg taatcaatac
    13861 caagataacc cggtcttgat accagttttg tgttttcggc acgaagatct gctttttctg
    13921 agaatccaaa tgtctcaatc ctgcaagtat gaccttttaa cacctgctcc agatgtttgt
    13981 catccatatt gacaatgcca agcttacact gctggaacag cagccctttg cagtgcatat
    14041 agtcttcaaa acttgcgtgt tctgccggtc cgatgtgatc cggttcaatg ttcgtgaaaa
    14101 taccaatctc aaacggaatg cctgccgtcc tgtgcagcat cagtccctga gaagatactt
    14161 ccatcacaac acagtcgcat cctgctttta ccatccggtc aaaataagac tgcacggtgt
    14221 aggactccgg ggtcgtattg gccgccggga tatgctcctc tccgatgatc gcctcgatcg
    14281 ttccgatcag tccgactttg tgtcccgcac tttccaggat ggatctgatc atatacgtgg
    14341 tcgtggtctt gccttttgtt ccggtgatac cgatcacttt cattttttcc gccggataat
    14401 caaaatatgc tgctgagatc attgccatcg catagcggct gtccgccact ttgatcaccg
    14461 tcacatcctt cggagcttct acttcattct gcacgaccag aacttttgct cctttggcga
    14521 tcacatctgc tgcgtatttg tgtccgtcag aaacggctcc tttgatgcag aaaaacagag
    14581 aaccttcttt tgcttttctg gagtcattga caagatctgc cacctcgaca tctacagagc
    14641 catttataca ttcatattct actctttcca gtaattttgt taatttcatg gctttcacct
    14701 ccatagacag taggatagca catctaccgt gctatttcaa acattttccg ttcctttcct
    14761 cttaccaggt tttcaggatt tcgatgatct cctgttcgta gttccccatg atgtcttcct
    14821 gtcttcccac ggtacgcaca tttgtctccg caccgtatcc accatcctca aattcccaga
    14881 tgtgatagct cagtccacgg tgctcaaaat caatgcttcc aagcccgtcc tcctccgtgt
    14941 attcgtagtt ccactgtttc tcttttaaat aagcctgtat tttttcaaga cgcatctgcc
    15001 tgctcccctt tcttctgcta tctgatgttt acataaagat gccttacgaa ttgctccgta
    15061 cttcgtactc ccacaatcct ttctacgctc cgcttcggtc ggtgctaaac atgtgccact
    15121 ggcacatagc accctggcat catcttatta ttctaacaca ggaaacagcg gtatcgcaac
    15181 tgtgggaact tccgccaaaa ttctttgttt tgcctccata acaaaaacct ccaaagcatc
    15241 cgttttttca tctgccctga aggttttcat tcttttctta ttgcggtttt atgaatttca
    15301 gtttctgctt atgccagtgt ttttccgagg ttgacgcagt ttgcttctgc ttcatcatcc
    15361 ggtgtttcgt tgcagatcac gctgtcacat gcaagggacg caccgtcgtt tttgcaggtt
    15421 tcttcccagt tctgcatcca ttctccatcg ccccatccgt aagaaccaaa cagtgcaatc
    15481 ttcttgccct ggagtttgga ttcgcaggaa gcaaacatcg gctcaaaaat ggtatcttcc
    15541 agttcttcgc atcccatgga cgggcatccg aatgctactg catcaaactc atccatttta
    15601 tcagcggaga aaccttctgc ttcaaatact tctacttctg ctccgcctgc ttttgcacct
    15661 tctgctactt tatttgccat gatctctgtg tttcctgttc cactccaata tacaactgct
    15721 actttgctca tgttttattc ctcttctttc ttctttatat ttttcatgct gtcgaacttt
    15781 tgccctccga cattttttaa caatatctgc ggagcatctg ctgcaaaatc atgtttgcag
    15841 tcttatgacg cgacagtaag tgtacggatg tcccgcccct gcttccacag tcttcgatag
    15901 acttctctgc attttttgta ttttgcaaaa agttttcttg tttcacattc attgcagtat
    15961 actttccagc agccggagca tttgtaattc tgccctgcgt tctcaataaa accaaccaca
    16021 tcttccagcg gatagtcaag gaacacacca atctcatgtg ggaatccttc tgcttttgca
    16081 agacgctctt tcagtctctg caatgcataa tcggcatctt tctgtacata gccgtatttt
    16141 ccaaggaagt tctgcacttt cggatcatcg agccgctcct gcagtctctg tttgcgacat
    16201 acatacatca gtgccttctg attctgtact ctcaggacaa caagtgaaat ccccctcgcc
    16261 tgtaattcct gattccagta ccgtacctgc tgctctggca tttcctccgg tgcaaaagac
    16321 caggtaaaca aatttgccgt cttcaccgat gccagagtcg gcgaacagtg ttcgatcaga
    16381 tatcgttcca gcatgagaaa cctccttttt acattgccac gcagtgttct tccagtactg
    16441 agtgcagtgc acttgcactg ctgctgtgga tccttgcaac cggaatgttg ttccgttttg
    16501 cttcctggat cgcagtgttt accattttgt gtgataccgt gttggtaaac aggatcagaa
    16561 gatccgggca accgattttt tttcttactg aaccatgctc ttttgcaaag acttttgctt
    16621 tgcagccatg gcatttgcag atgtcagcgt actgacagac cattctttcg tttcctccaa
    16681 taatcactac actcatgttc ttatcctctc catctttctt aaaattataa gatgccaggg
    16741 tcaaaaacct gaagccacga cgtgcttgca caggattggt tttatgacat ccttgttccg
    16801 atgcttctta caactacctt aaattagttt taactaacct atgattttaa aaaaacggac
    16861 tgccaagact atcctgaaac agtccgtttt ccacctttat ctggcacaac acatgtcaga
    16921 attccaggct atttttttta tttcctgatc ttcaatatat ccgcacgctt ttagacctgc
    16981 ctgaaatact gcgtccagtt tctctgctga gagacagtac gttgtgcggt cactcagttc
    17041 cacgatgcag ctgtttgcag cttttttctt ttctgcttca cagctgccat tttcgcacag
    17101 ttcttcacat gccactttgt acatgttctt tctgctgatg gcaccgattt cttccaacat
    17161 attcaccata cggtaaaccg ttgcaaatcc aatctttgta tcttgtccgg aagctttgta
    17221 ataaatttcc ttgcagcagg agcagtcctc ctgcaggatg atatccagga tgatcagtct
    17281 ctgtcttgta atcctgcagc cacgctcctt tagcttgcgg atgatatatt ctctctgtgg
    17341 cacaagcgtc cctcccttaa tggcagtgac cgccacaatt cttacagtct ccgccgcact
    17401 gaatggattt tccgtttttc ttatcgcgta ccatactgcg gacagcaagt gctacaactc
    17461 ctgccacaat aataccaact acaactgttc ccataaaaaa tcctcctttc aggctttcgg
    17521 caaaatcttt attttgccct ggatgtcttt acggatacgt tcagagtctg actctctttg
    17581 taaggacgag ccagcaggta aatgaatgct acaataactg cgatcgccac aatcgtccag
    17641 atactgaatc cggctcctgt gaacagtccg ccgatctgat ttacaaccag agataccaga
    17701 tatgcaaaac cgcactgata tccgactgct gtccagaacc attttgtatt gttcatttca
    17761 cgtttgatcg cacccatagc tgcgaagcac ggtgcacaca gcaggttgaa tgccaggaag
    17821 gaatatccgc ttactgctgt gaaagctgct cccatggttg cgtaaacgct tgcgtctccg
    17881 cctccgtaaa ggatgcccag tgtaccaaca atgttctctt ttgcaaccag acctgtaaca
    17941 gatgcaacgg ttgcctgcca gttgccccat ccctgtggaa tgaagatcca gcagattgct
    18001 ttaccgatcg tagccagaat actgtaatca atctggtctt cggaaagcat ctggaatttg
    18061 ccgtccatga caccaaagta agttgtgaac catacagcga tggtagatac caggataatc
    18121 gtaccagctt ttttaataaa tgaccagcca cgttcccaca tacttcttaa aacgcttccg
    18181 atcgtcggca tatggtatgc cggaagttcc attacaaatg gtgccggatc acctgcaaac
    18241 atttttgttt tcttcaggat aataccggag cagatgatcg ctgcgatacc aaggaagtat
    18301 gcagatggtg ctacccagga tgctccaccg aagattgctc ctgcaaccat ggcgataaac
    18361 ggaagttttg caccacatgg gataaaggtt gttgtcatga tcgtcatacg acggtctctt
    18421 tcattttcaa tcgtacggga agccatgata cccggaacgc cacagccgga gccgatcagc
    18481 attgggataa aggacttacc ggaaagacca aatttgcgga acactcggtc catgatgaag
    18541 gcaactcgtg ccatgtatcc gcatccttcc aggaatgcca ggaagatgaa caggatcagg
    18601 atctgcggaa cgaatccgag aaccgcacca agacctgcta cgataccatc caggatcaga
    18661 cctctcagcc attctgcaca gccgactttg tcaagtacat tctcaaccag aaccggaata
    18721 cctggaatcc atacgccgta gtctgccgga tccggagctg tgtaatcata tttatccatg
    18781 gttgctgctg cttcctggaa atctgcaccg gtagaagtta cttcttcgtc tgccattgtc
    18841 tcttcatcta ctacagaata ggttgctgta tctgcatcag aaatggttgt tgcaaactct
    18901 gtcagagctg cttttgctgc tgccggatca taatcatctg cttctgaatc cagagctgtc
    18961 tgaacgcttt ctacgtctac gccctgctcg ctttcataat ccaggaaacc atttactatc
    19021 gtttctgcat ctgtgtattc atctgcaact tctgtgtagg acgctgttcc gataccgaac
    19081 agatgccatc catcaccgaa tacaccatcg tttgcccagc ctgttgcgat gtcaccaacg
    19141 gttgttaccg atacaaaata taccagccac atgactgcag caaagattgg cagtgccagc
    19201 cagcggtttg ttacaatctt atcaatctta tcagaagtag tcagtttttc ttttctgtct
    19261 ttctttaagc attctccgat aatagaagnn nnnnnnnnaa gtatatctct cgtttgtgat
    19321 aatactttcg gtatcatcat caaatttttc ttccatctca gcgatctctt tggatacatc
    19381 cacgctggtg ttcatctgat cctggatctt atcatctttt tccagaagtt tgatcgcaaa
    19441 gaatcttctc tgtgcatccg gtacaacacc gaccagtttg tcttctactc tctcaatgat
    19501 gtcctctgca tcttttgtaa attcatgaac cggaatggaa gttttgttct tttgtgccag
    19561 agcgacagct ttttctgctg ctttctggat tccggtacct ttcagtgcgg aaatctcaac
    19621 tacctcacag ccgatctttt tgctcagttt gtcaacatag atcttatcgc cggatttttc
    19681 aacgatatcc atcatgttaa ccgccatgat caccggaata ccgagttcca gaacctgtgt
    19741 ggacagatac aggttacgct caatgttggt tccgtctacg atattaatga tcgcatccgg
    19801 tttttcgttg atcagataat ttctggcaac cacttcctcc agtgtatatg gagacagaga
    19861 ataaatacca ggtaagtcca taatggttac atctttatgg cctttgagct taccttcctt
    19921 tttctcaacg gttacaccag gccagtttcc cacgaactgg ttagaccctg tcagtgcgtt
    19981 aaacagtgta gtcttaccac agttcggatt tcctgccaat gcaattttaa ttgacatact
    20041 ttcctcctct ttctcaaaaa aacaatcacc cttcgggctc atttttccag gagctgattt
    20101 acacagctta tttttatttg tgtttgctta tttatatttc gcatctctta tttatgttag
    20161 cattatctaa tccatgagta aaaaaaatta ttctacttca atcatttccg catctgcttt
    20221 tctaagagat aattcatagc cgcgaacggt tacttcgatc ggatcaccaa gtggtgcaac
    20281 ttttcttaca taaatgctga cgccttttgt gattcccatg tccatgatcc ttcttttgac
    20341 cggaccagca ccgctcagtt ttttcactgt gacagtctgt ccacaggcaa cttctcttaa
    20401 tgttttcatc tttgcctacc ctttctgttt tctgattttt ccggagatag catttttcat
    20461 cagaccataa tcttgttggc catatctttg ccaatggcaa ctctggaatc ttttacgttc
    20521 acgatcaggt ttccttctat ttcagaaaca actgtcacag ttccgcctgg cacaaatcca
    20581 agtttctcca gaaatcttct ggtttcttct ttgccaccga cctttttgat cacactcggt
    20641 tctcctgtgt tcaccatcgt taaaggcatc atttgcaacc tcccttttta taaaagtaag
    20701 ctcaaaacag gctccttttc tttttcttca tggttnnnnn nnnnntagaa aatgtaactg
    20761 ccatgtattc tgtttcttgt tttcagctgt tgtttctttt attccgattc atccagcaga
    20821 ttcttcatgc tctgcagtcc gatcaccagc gtagatgtat tgtgcagcag tgcggatgtc
    20881 gtaggcggaa gtacgcctgt cacgccgcat acgatcagtc cggtattaaa tccgacgata
    20941 atccggtagt tcctgcggat acgctgcatc agtgcatcac tcagtctctt gagtttgacg
    21001 atctctttga ggtcgtctgc accgacagtg atatctgcca cttcccttgc gatctctgct
    21061 ccttcgctga tcgcaatgcc gacatctgcc gcagacagtg ccggtgaatc gttgatgcca
    21121 tcgccgatca taatgacttt gcgtcctgcc tgtttttccc tttcaacaaa gtttgctttg
    21181 tcttccggaa gtacttcgga ataatattca tcgacaccga cacgttttgc gattgctttt
    21241 gcggtacggt cactgtcgcc ggtcatcata acgatcttag aaagtcctgc ttcacgcagc
    21301 tgttcaacca cctgagctgc ttcttttcgg agcggatctt caatgcagat cacggctgca
    21361 agctcgttct ggattgccag atacaggtgg gaatattctt ccggcaggtt ttcaaacctg
    21421 tcttccattc cttccggtat cgtgcacttt tcatcctcga aaatgaagtg atggcttccg
    21481 atcacagctt ttttgccttc gatcgtagta gaaataccgt gtgccacgat atactctacc
    21541 tttgtatgca tctcttcgtg atccagatgc cgctctttgg ctgcatttac cactgctttt
    21601 gccatagaat gagggaaatg ctcctccagg caggctgcga tgcgaagcag ctcgtcttca
    21661 ctgtatccgt taaaggagat cacttttgca acgatcggtt tcgcctcggt aagcgttccg
    21721 gtcttgtcaa atacgattgt gtctgcttct gccactgctt ccaggaattt acctcccttg
    21781 accgtgatct tgaagcttcc ggcttcgcgg atcgcagaaa gaacggatac cggcattgcc
    21841 agtttcagtg cacaggagaa atctaccatc agcaccgaga gagccttggt cacatttctg
    21901 gtaaagagcc aggtaagggc ggttcctgca agtgtatacg gaaccagctt gtccgccaga
    21961 tgctctgcct tgctctccag tccggatttc agtttttcag actcttcgat catggctgcg
    22021 atcttctcat accggcttga tccgcctgct ttgcggacaa gaaccgtgat ctcgccttct
    22081 tctacgacgg tccctgcaaa taccgtattc ccagctgttt tgcgtaccgg tgcagactct
    22141 cctgtcaaag aagcctgatt aagcatcgcc tctccgtccg atacagttcc gtcaaacgga
    22201 accacattgc ccatatgtac cacgatctga tcattttctc tgatcctgga tgcggatacg
    22261 agtacttcct ggccggatgc ctcttttatc catactttat ctacattcag tgacatcgtc
    22321 cttgccagat catcaacgga tttcttgtgc gtccactctt ctaacagttc tccgatgcca
    22381 agcaggaaca tgatcgaacc tgcggtcgcc gtgtcaccac gcagtacaga aactccgatg
    22441 gctgttccat ccagtaccgg aacttcaatc ttgccttttg caagtgtctg aattcccttc
    22501 cagatgtatt tgattgagca gaaaccggtg taagctgcgg cgatcggtgc cggcagaaat
    22561 acttttcttc cgtaatggaa cagaacttta ttaaccagtt tttcctgata ctgggaattc
    22621 agctcccttc cggaatgttc cagtatgcca tccggaactt ttacatcttt gcagcgatag
    22681 gcagctacct tctttaataa agtactgcgt tctccctgat aacagatcac tgcatctgcg
    22741 gtacgttcat atacaactgc ttttgttacc tgaggcagct cttccagata atactggaaa
    22801 agatctgcct ctttataaga caggcgtttc gcctccatat ggatacggat cctgcctctt
    22861 atctcgtgtt taattctgaa tttcaatctt cctcaaacct ctattctgca gtttcctctg
    22921 ttgtctcttc tgccgctgtt tcttctgttt ccgcttctgt ttcttcagcc tctgcttcca
    22981 cttcatcctc tacttctttt aaatcttcat catcataaac agcttctttt gccgcacgct
    23041 cttcgttgat ctgttttgct tcttcgtaga tgtcacctgc attttcctga actttcgtta
    23101 ccgtctgcat aacgcnnnnn nnnnntgctt taataccggc tgtaccaaaa gcaacacctg
    23161 ctgcaaaaat tcctgttttt ttccaatctg cattttttaa acattttaac attcttttgt
    23221 acctcctagt tttgttttat ttttgaattt ccttgcatta tatcactctg atttgtgttt
    23281 ttctattatt tttttctttt attattgata atttttatca atagatttgt ctatggataa
    23341 actttataag gattcgatct ttttgcaggt tcccggatca taaccgctgc atctgacgat
    23401 accctggatc tcctgtgtgc taatctgctc ttttgcgtaa acttgtgctt ttttctgttt
    23461 cagatttact ttcgcataaa agcctttctt ttcgttaaaa gcattctcca ctctcttttt
    23521 gcactgactg caggtcatac cctccacttc cacttcaaaa tgatatggat aatgagagac
    23581 atcggtatcc tctgctttta cttttttcac atcatcaccg gttccgcagc agccgtttga
    23641 aacacgtttc atggaactgc ggattccgaa gaaacagatc acaactaaaa tagcacagat
    23701 aataatggtt gacatataca tccctcctga cgtaacgctg aaaaaattga taatcttttt
    23761 cacttctttt tgcgtctgtt ttcttcgcca agtgcgatgt catttacgat ctcacctgcc
    23821 atgatcagtc ccatgacaga cggtgtaaag gccgtacttc ccgggattgc tctgcggtct
    23881 gtacactttc tttgtacacc cggcgggcag acgcagtgtg ccttgcagct gatcgacata
    23941 tcttcgatcg gcgtgcgtgc cttctctttg gaatacacca cttttagact gtccacttta
    24001 cgtttcttta attccctcct catgactttt gccagcgggc agacggatgt ctcgtaaata
    24061 tctgccactt caaacagttc cggatggatc ttgtttccgg ctcccattgc ggagataaca
    24121 ggtactcctg cttcttttgc cgccatgaca atatcgattt ttgccgtcac ggtgtcaacg
    24181 gcatctacca cgtaagtgca tgacgcaaaa tcaatcagat cacgctggtc cggcagataa
    24241 aagcagttat gcagatggat cctggcatgc ggattgatct ccagaatacg gtcacgcata
    24301 acttctactt tttgttttcc gaccgttttt ctggtcgcaa tgatctgacg gttcagattt
    24361 gtcaggcaga ctttatcgtc atcgatcaga tcaaaagtac cgactccgct tcgtgccagt
    24421 gcttctgctg tataaccgcc gacaccgccc agtccgaaaa ttgcaactct cgcatttgcc
    24481 aggcgttcca ttgcctcttt tccataaaga agctctgttc tcgaaaactg atttaacatg
    24541 attcataatc ctccaaaaat gaatgtctga ctccgttttt cttatatgcc acaacggtgt
    24601 ccagatttac gttctcaata ctgcggaaaa tattggtctc tacataagga ttgacctttt
    24661 tcatttctgc gatcatatgc ttgatctcca tacgcttcga tgcactttcc ccatttggcc
    24721 tgcaggtcgt acaagtgtct gtaaatctgc atgcacactg aataaagtgc aggctgttat
    24781 agtctctcca gtgtttgata tcctcttccc ggatcagata aagcgggcgg atcagttcca
    24841 tgccctcaaa atgaatgctg tgaagcttcg gcatcatggt ctgcacctga ccgccgtaga
    24901 gcattcccat caggatcgtc tcaatcacat cgtcaaaatg atgtcccagt gcaatcttgt
    24961 tgcatccaag ctcttttgct ttgctgtaaa gatgaccgcg ccgcattctt gcacagagat
    25021 agcacggtga tttttcaata ttataaactg cttcaaaaat gttcgtttca aaaatcgtca
    25081 gcggaatgtt taagattttt gcattctgct cgatgatctg acggttgcgt tcgttatagc
    25141 ccgggtccat cacaagatag accacttcaa atggaaattt gtgatgacgc tgaagttcct
    25201 ggaacagttt tgccatcaac atggaatcct ttccgccgga aatacagaca gcaattttat
    25261 ctccttcccg gacaagctga tattcattga ttgcttttgt aaatttgctg aaaatagatt
    25321 tatggaattt tttgcggatg ctcttttcaa tatccgcatt tctggctgtc tcctgaaaat
    25381 ggttcagcag ccatttatca taaccgcctt ccagactgca ggcatcaaat ccaaacccgg
    25441 aaagcagttc tgcctgatcg gtactgatca caccgctttg gcagtataca atgatctttt
    25501 tttctgtctg aagttcgtct tttcgctcaa tcagctcatt tgccggaata ttgagggcat
    25561 cttttaaatg tccatgatca aaagcttcca tgcttcggat atccagcagc tgatacgttt
    25621 cctgttccat attttcaagc tccgcaaggg agcagctttt tacgttttcc aaaatttttc
    25681 tcctgtcttt taaaattccc ggtaatcacc gcggtctgtg acgatctcat agccgatgct
    25741 gcggattctc gcatcaagac annnnnnnnn ntctgccgcc tcatccccgg tacacagttt
    25801 attgtcatag agcatataat ttttccgcac agataccggt gaaagattcg gcatcacgac
    25861 atttgccccg gaaaggattg ccttttcacg ccctctcgga tcgatcgtgc caaccgctgt
    25921 tgtcgccgga atcatagcgt ttggattcat cagccgcaac atggcaacca gattcagagt
    25981 cagatccaga ctgcctcctg tttcttttgc aaataccgtg tctttgtgtg ggataaacgg
    26041 tccgatgccg atcatatgcg gcctcagttt tttgatataa cacagttccc gtgccagata
    26101 ttctaattgc cagcccggtg aacccaccat aaatccacat ccggtctgat aaccggtttc
    26161 ttttaatatt tcaaggcagt gcagtcggtt ttccaggctc atctgctttg gatgcagcat
    26221 cgcataatgc tccggaacgg atgtctcttc tcgcagaaga tagcggtctg cccctgcttt
    26281 gtgcatcaca cggtaagatt cttctgaacg ttcgccaagc gagagcgtca ctgcacagtc
    26341 cggatattct ttcttgatcg agcgaatgat ctcacacatc cgttcatcat tgaaataagc
    26401 atcctcgccg ccctgcagca caaaagtccg atagcccagt tcatagccca tcttacagca
    26461 ctccatgatc tgtgaaagtg tcagacggta acgcactgca ttctgattgc ccctgcggat
    26521 accgcagtag agacagtcat ttttgcagta gttggtaaac tcgatcagcc ctcgcagata
    26581 aatctttctg ccaaagtgcc tgaccgcttc ttctgctgct gccgcccgca gatacgcact
    26641 ggattcttca tccagatgct ccagaagata ccgaagctcc tccatctgtg catcctgttc
    26701 ttttctcagt ttttctatga tcggtctgca atccatgcca ttcccccaac cctttttctg
    26761 ccgttgtttt tattatcgct gttattctac atggaaaacg caaaaaagta aagccgactt
    26821 tttatgtcag ctttaccgct gttttgttct tttaaaaata aggatgcctg cagattgctc
    26881 cgtttcgttt caggctttta acccgggcat catcatatat aat
//
LOCUS       DAWDWH010000008        26655 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_1090_length_26655_cov_13.643872, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000008 DAWDWH010000000
VERSION     DAWDWH010000008.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 26655)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 26655)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..26655
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1090_length_26655_cov_13.643872"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3086..3095
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4109..4118
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13935..13944
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16359..16368
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    25000..25009
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6124 a         6977 c         5424 g         8080 t
ORIGIN      
        1 aatgtgtgcc ctcgcggagc gtttatctca gtcggagatt gtactcccac tgagaaagac
       61 ttgttattac tcaccacttg cagaagtggg agtcttctcg actgagataa aaaacgccag
      121 agacaagcag ctttgaaatc acccttttac aggtatatta tgattttggc ttttatctct
      181 ggcatttatg attttcatct ccggctgcat gagctgccag gcaactgtac agctctgtag
      241 ctcctacatc cttttatact tcaaacagca ggtcaccgta ggatggcatc ggccactctt
      301 ctttatctac gatcatctcc agcttatcca ccggaactcg cagttcttcc atatctttaa
      361 acacaacttc atgataatac tctgcctgtt ctcttccgtt ctccatggct gccgcctgat
      421 ctgctgcctc ctgcagtttt gccagtgcca gttttgtctg agaaagaagg gctgttgtat
      481 ctttgagcat gttcatcgga acagtcacat ctgctccggc tgtcttgatc tcatttaccg
      541 taccggcaag gtttctggaa aaacgcatga cggccgggat gatatgcttg ctggcaatat
      601 cgatcatggc acgggcttcg atattcagtg ccttggagta tccttcatac tggatctctg
      661 cacgggaacg gagttcagct tccgtaaata cgccgaattt gccaaacagc tggatcgctt
      721 tatcggtcac aagtgccggg atcgcttcca ccatggaatt gatgttcggc aggcctctgc
      781 gttttgcttc ttctacccac tcttcagaat aaccgttgcc attaaatacg atcctctgat
      841 gttctgttgc atattctttg atcaggtcat gcacggcaag ctcaaaatct tctgcctttt
      901 ccagacggtc gcaggcatcg gagaatgctt ccgctacgat cgtgttgatc accacgttac
      961 attctgctac ggaatctctg gaacctacca tacggaattc aaatttattt ccggtaaagg
     1021 caaacggtga ggtacggtta cggtccgttg catcttttgc aaaatccgga agagtcttaa
     1081 cgccggtatg taattttccg ccttttaagc tgtgtgttgc agagccggtg ctgatcagct
     1141 gttccagcac atcttccagc tgttcaccca ggaatacgga gataatagca ggcggtgcct
     1201 cattggctcc cagtctgtgg tcatttccga catcggctgc ggattcacga agaagatctg
     1261 catggcggtc tacagcacgc aaaatacagg tcaggatcat caggaactga acattttcgt
     1321 gcggtgtctt gcccggatca agcagattaa tgccgtcatc tgtggtgatg gaccagttgt
     1381 tgtgcttacc ggaaccattg actcccgcaa atggtttctc atgaagaaga cactgcagtc
     1441 cgtgacgctc tgccacctgt ttcaaagtct ccataatgat ctggttgtgg tctgctgcta
     1501 cgttgcactc tgcatagatc ggtgccagct catgctgtgc cggtgcgact tcgttgtgct
     1561 gtgtctttgc agaaacaccc agtttccaga gttcttcgtt tacttctttc ataaaaccgg
     1621 caattctctg acggataact ccaaaataat ggtcatcgag ctcctgacct ttcggaggca
     1681 tggcaccaaa gagtgtacgt ccggtaaaaa tcaggtcttt tctctttaaa tatttctgcc
     1741 ggtccacgat aaagtattcc tgctctacgc caacggaagg tgtcactttt ctggatgtcg
     1801 tattgccgaa cagacggatc agtctgagtg tctgtgtctg caatgcctgc atggagcgaa
     1861 gcagcggtgt cttctgatcc agtgcttctc ctgtatagga acagaatgct gtcggtatac
     1921 acaaaatcgc acctgcactg tcttcacgta caaatgccgg tgaagtgcag tcccaggctg
     1981 tatatccgcg tgcctcaaag gttgctctta atcctcccga cgggaatgaa gaagcatccg
     2041 gttctccttt gatcagctct tttccggaaa agtccatgct gaccgttccg tccgcatgcg
     2101 gtgctgtaat aaatgcatca tgtttttctg ctgtgaatcc agtaagcggc tggaaccagt
     2161 gtgtgtaatg cgttgcacct ttttccactg cccagtcttt catagcccca gctacgactt
     2221 ctgctgtgat cggatccagc tcttttcctt catcaatggt cttatgcagt tctttgtaca
     2281 cctttttcgg cagacgctcc tgcatgatct tgtcattgaa tacattcgaa ccaaaaagct
     2341 ctaccacatt gatcttttct ttgttcttgt cttccatttc ttacaatcct ccatcgtgac
     2401 ctgctgcttt tcatatccaa aaagcttttt tcgtttgctt ttgtctatcc ttccaaatca
     2461 taccttattt ttcactgttt tgcaactaac ttatgcaatc ctgccaggct ctgctgacat
     2521 ctgcctgaga gaatcgctgc acaccaaaaa aggaacagaa atcttttgtt gatttctgtc
     2581 cctttcatta cttttcggga tggaataaca gggtggggaa accactgtat ctttacattt
     2641 tttatacttc gaacagcaga tcggaatagg atggcatcgg ccatactttc ttatctacca
     2701 gtacttccag tttgtcaacc ggtgtacgaa gggctgccat agccggtaca acgctctcgt
     2761 ggaagtatct tgcttcagct gccatatcgc ctgcttcggc tgctttgtcg gtcacttctt
     2821 ccagtgttac cagggctgcc tttgcctctt ccagcagttt gcttacatcg ttcagcagat
     2881 ctgtctgtac ggctgccggt gcacctgcac ctttaactgc caggatagta tctgccagag
     2941 atttggtata gccgatgact gccggcagga tctgtttgga agccatgtcg atcatcgtct
     3001 ttgcttctat attaatagcc tttgcatagt tctcatattt aatctctaca cgggactcaa
     3061 gttctgcttt tgagaaaact ttgtannnnn nnnnnaactt tgtatttgcc gaacagttct
     3121 actgccttat cagttgccag gcacgggatt gcatccacca tggatttgat gttcggcagt
     3181 cctcttcttt ctgcttctgc tacccattca tcggaataac cgtttccgtt aaatacgatt
     3241 ctctggtgat cggttgcata ttctttgatc agatcatgta cagccagttc aaagtcatct
     3301 gctttttcca gtacatcgca tgcctgtgag aatgcatctg cgacgatcgt gttcagtacc
     3361 acgtttggtc cggcgatgga atcacgggaa cctaccatac ggaactcgaa tttatttccg
     3421 gtaaaggcaa acggtgaagt acggttacgg tccgttgcat cttttgcaaa atccggaagt
     3481 ctcttgacac cggtatgcag tacaccgcct gctttactgt gtgtagcagt tcctgtgctg
     3541 atcagctgtt ccagtacatc ctccagctgt tctccaagga atatagaaat gatagccggc
     3601 ggtgcctcgt ttgctcccag tctgtgatca tttccaggat cggctgcgga ttcacgaagc
     3661 agatctgcat gctcatcgac tgcttttaag atacaggtca gtaccataag gaactgtaca
     3721 ttctcatgcg gtgttttgcc cggatctaac agattgatac cgtcatcggt tgtgatggac
     3781 cagttgttat gtttaccgga accattcaca ccgtcaaatg gtttctcatg aagcaggcac
     3841 tgcattccat gctggcatgc tacttttttc agtgttttca taaccagctg gttgtgatca
     3901 actgcaatgt ttgcttctgc atagatcggt gccagctcgt gctgtgccgg tgctacttcg
     3961 ttatgctgtg tttttgcgga gataccaagt ttccagagtt cttcgttgac atctttcata
     4021 taagaagcaa tacgctgacg gatggttccg aaataatggt catccatctc ctgtcctttc
     4081 ggaggcattg ctccgaacag ggtacgtcnn nnnnnnnngg taaaaatcag atcttctctc
     4141 tgcagatatt tctgtttgtc aaccaggaag tattcctgtt ccggtccaac ggacggtgtt
     4201 acttttttgg aggttgtatt tccaaacagt ctcagaagtc tcagtgcctg ctcattgatt
     4261 gcttccatgg aacgcagcag cggtgttttc tgatccagtg cctcacctgt gtaagagcag
     4321 aatgcggtcg gaatacagag gatcgctcct gctgcatctt cacgtacata agccggtgaa
     4381 gtacaatccc atgctgtata tcctctggct tcaaacgtag ctcgaagacc tcctgacggg
     4441 aaggaagaag catccggttc tcctttgatc agttcttttc cggagaactc catcaatacg
     4501 gtaccgtcct gtctcggtgc tgtgataaat gcatcgtgtt tttctgctgt tacaccggta
     4561 agaggctgga aaatgtgtgt gtaatgtgtt gcaccttttt ctactgccca gtttttcata
     4621 gcttctgcca ccacatctgc taccatcgga tccagttctt ttccctcatc aatggttttt
     4681 ctcagtgctt tatacacttt tttcggcaga cgctcctgca tcactttgtc gttgaataca
     4741 ttttcaccaa aaatctttgc aacattcatc ttttcagtca tgtcttcttc tcatcctttc
     4801 tgtgataagc cggaacagaa tttctctgta acctttctgc ttctttacag aaaattaaaa
     4861 aagacgctcc acccctttcg gggcagaacg tctttgttct ctcgctcatc atttttaatt
     4921 ttgctactac catactccac atttttggca aatgcaagta taatttaatt tttctacaaa
     4981 atttataaaa tttcagagaa ataattctca ggctttgtcg atagagccga aaatttccat
     5041 tttttctttt actgtagctt tgattgcttc gaatccagga gccagaagtt tacgcgggtc
     5101 aaatcccttt ccttccagat ctttaccagc ttcgatgtat tcacgtgtag ctgctgcaaa
     5161 tgccagctgg cactctgtat ttacattgat ctttgcaacg ccaaggctga ttgctttttt
     5221 gatcatgtct gtcgggatgc ctgtaccacc gtgcagtacc agaggcagat ctcctgtcag
     5281 agcctgaaca gcgtccagag tctcgaagct taagcctttc cagtttgccg gatatttgcc
     5341 gtggatgtta ccgataccag ctgccaggaa gtcaataccc agatctgcaa ttctcttgca
     5401 ttcctgagga tctgcacatt cacccgcacc tataacgccg tcttcttcac cgccgataga
     5461 accaacttct gcttccagag acataccatg ttctttgcag atttctacca gtttctttgt
     5521 gtttgcaacg ttttcgtcaa tcgggctgtg agaaccatca tacatgatag atgagaatcc
     5581 ggcttctaca catttcaggc atccttcgta gctgccgtga tccagatgca gagcaaccgg
     5641 aactgtgatg ttcagttctt ccatcatacc atttaccata cctacaacag ttttgtatcc
     5701 tgtcatatat ttgccggcac cttcagatac accaaggata accggtgagt tgttctcctg
     5761 tgctgtcagc aggattgctt ttgtccactc aaggttgttg atgttgaact gtcctactgc
     5821 ataatgacct gcttttgctt tctgtaacat ttcttttgct gaaactaaca tattttttac
     5881 ctccacactt tgtgattttt ggtgttggct ttattataac ctaattgcac caaaaagaaa
     5941 atactttttt agtcaaatat cccctgtttt tttctgagta ctatatttca ctctcgccaa
     6001 actgatgatt gacaatgata tccactgctt tctgatgaca gcagatttca acttctttat
     6061 gatcgccgcc atattcacca tccagcgtcc atggaattgc aatctcggaa cgaatgacaa
     6121 cctgtctggt cttatagctg ataatatagt tgctgtccat ctccccgcga aggaggcttg
     6181 taataatctc ctgaagttct atggcgtttc gtggcgtttt gatcatcgtc acttcaaaca
     6241 gaccatcatc cagccggatg ttttttcctg taatgctgcg gaaaccacct acggattctg
     6301 aattggtgat cattccataa ataaattcat ctctgatctt ctcaccgttc agttcaatct
     6361 ccacttcata agatttaaca ttaaaaatac acttggcacc ttctaaaaca taagcaagat
     6421 ggccaagtac atttttgaca tcctggtttg tctgataaga cacttcggta aacattccaa
     6481 atgcagcgat atatacaaaa taatcactgt taaaagagcc gacatcacac ggataaacgt
     6541 tgttctctac gatacactgt gccgcttccg tcatcctttt cgggatctgc aggctgtttg
     6601 caaaatcgtt tgtgcttcct gccgggatat agccgatcgg gcgtctgatc tcgctgttca
     6661 tcataccggt caccacttca tccagtgtcc cgtcaccgcc gctgcaggcg acaagatcaa
     6721 actttgcagc ccgtttctcc acaactctgc tcgcatcctg tggtttctgt gtcacataga
     6781 gcgtgacatc caaccctcct ttgataaaaa tatctgcgat atccaacagt gcatttctga
     6841 tctgtccttt tccggatctt ggattcacaa taaataaaac ttttttacgt ctcatttttc
     6901 attccttcca ctttcaccgt atttcgacat attgtactat catttttttc aacagacaag
     6961 taccctgact gtttttaaaa cttaagtttt cataaaatct gatattcctg tgcagaatac
     7021 tgtaccgttg aaggcgacct cacaatggaa agcttactgt cgtcagaaca gtcctttcat
     7081 aggaattttt cagattttag aaatcagaat ttccggattt catctcctcc gtcatcggta
     7141 ttttcaattt tgcggatcac agcataatag ccgaaatttt cattgacttt gatatatacc
     7201 ctctctccga ctttatgtcc gagcagggct tttcccatcg gggactccgt gctgatgtgg
     7261 cctttcatgg aattgccgcg gatcgatgtg acgatcttgt aggtttcttc ttcgtcatca
     7321 tcttccatat agagtgtcac cgtattgttg atcccgactt cgtcggattt cgattgatcc
     7381 gatacgattt cggcattttt taacattctc tccagatagc ggatacggct ctcgttcata
     7441 tttttatctt ttttggcggc atgatactca aaattttcgc ttaaatcccc atgtgctctc
     7501 gcctctttga ctgcctcgat cgcatttttt cgaacggtca gttttcgata ttcaatctct
     7561 tctttgattt ttttgacatc attttccgtc agctgttcat gtaacatatc ttatccctgc
     7621 actctcacaa cataatcttc ttttctcggt tttgccgggg cttctccgcc ctctgccgca
     7681 tagcctaaaa tacagtgacc gattccggca taatcacctt caatgcccca ttttttcaga
     7741 agagcttttc cttcttcact ttcaaactct tcttttgcac ggtggatcca gcaggacgcc
     7801 acaccaacgg catgtgccgc attcatcaga ttgcccatga caagtgaacc gtcttccaga
     7861 taggtgtttc tgttcttgtc tgccagcacg atcagcaccg tcggtgctcc gtagaatgga
     7921 tcagatgtca cgcccatgat cttcgcattc attttgctca gctcttcaat ggtttcttta
     7981 tcctgaacaa ccacaatctt cggggactgc atacccatgc cggttggtgc aaacacgccc
     8041 gcctcaagga tctgttccag ttcgctttct tttatctgct ccggacgata tctccggcag
     8101 cttcttcttg tctttaaatc ctgtaatgtc tgattcatca tgaagcctcc ttgtgtataa
     8161 cagtattatg attctctcca cttcagtaac gccctgcgga ttgcttcagg cttttgcatt
     8221 ctcacaatcc tgcattacct atagcttcgt gatttttccg catttcatgc tttcaaaatc
     8281 acgcaacatt cgaaatccgc acatttttct ttgaaaaatg gcttctcatg ttttttattt
     8341 cagtaacgcc ctgcggattg ttccatgctt cgcattctca caatcctact ctaaattcgg
     8401 atatcgcggg gcatacgccc cgcttttatc cgaattcaga gcggggcatt aagccataaa
     8461 agcactcctg tgcaagcaca tcgtgctttc aggcttttgc ccctggcgtt acagacatta
     8521 taccaccttt ttgatttttt tgtagtcaaa gtattttttg tgcggtataa tcttttaggt
     8581 aaatgccgat ctttaatata cacaggaggt tctttgaaat gaaagacgtt tttatatctg
     8641 attctatcgt atacgttggc gtagatgata agaatattga tctttttgaa agtcagtatg
     8701 tcgttccaaa cggtgtttct tacaattctt atgtgatctt agatgacaag atcacgatca
     8761 tggacaccgt agatcgacgc ggtacggaag gatggcttgc caacctgaag gaaacgctgg
     8821 gtgacaaaaa gccttactat gtggttgttt cacacctaga accggaccat gccggaaact
     8881 taaagacact gctggatctg tatccggaca tcaaggtgat cggaaatccg aaaacgttca
     8941 cgatgatcgc acagttcttt gatgtggatt taagcgatga ccgcaaggtg gttgtaggcg
     9001 aaggcgatac ggtaaatctc ggtactcaca cgcttcagtt ctttatggct ccgcttgttc
     9061 actggccgga agttatggtt gcttatgagc agtccgaaaa gatcctgttc tccgctgacg
     9121 gtttcggtaa attcggtgcc ctggatgcag acgagtcatg ggaagacgaa gcaagacgct
     9181 attttatcaa catcgttggc aaatacggcg tacaggttca gaatcttctg aaaaaggctg
     9241 ccactctgga tatccagatg atctgcccgc tgcacggtcc aatcctgaaa gaaaaccttg
     9301 gctattacat aggcaaatat ctgacctgga gcagctacga accggaagaa aagggcgttg
     9361 tgatcgcatg tgcttccatt cacggaaata ccatgatcgc tgcaaagaaa ctggcggaga
     9421 tcctgaaagc aaaaggcgag aaaaatgtgg ttctctatga ccttgccaga gatgatatgc
     9481 cgtctgccat cgcagatgct tatcgttatg acagactggt ggtcgctgct gcttcctacg
     9541 acggcggcat cttcccttgc atggagacat tcctgcgtaa actcaaggca aagaacttcc
     9601 agaaacgtac cgtcggctac atccagaacg gaacctgggc tccttgcact gcaaaacaga
     9661 tgcaggctat catgggcgag ctgaaaaaca tcaccaatgc agaaccggtc gtaacgatca
     9721 aatctgcaat gaaagaagcg gatattgctc agctggaagc tctggctgat gctctgatgg
     9781 cataaattgt ataaaaaacg cacccgtagt gctcttgaaa agcatttcgg gtgcgttttt
     9841 cttttacgat attgaatttc aatctcttct tacagatcga tcatattgat atctgctttt
     9901 ttctttgttt tcttcggtgc aggctgtgct gcattgcctg ttggctgcgg tgtatgtttc
     9961 acctgtgcct cctgtggctg tgcactggat ggatcatcct gcatttctgc catctcctgt
    10021 tttgcctgct tctgctgacg ctttttctct ctgcggcttt tccggtctgc ttttaccagc
    10081 ccggtctttt tattttcacc gaatgcttct tccactgccg gagcagcttc tgtttccgga
    10141 tgtactggct gcggttcttc ctcctgaacc ataccggttg cctcctgctg ctgcgtcggt
    10201 gcataataat tttccgttgc cagataatac tcttttcttg caaactcgtt ggtctgcata
    10261 tccagtcttt cctgcgtatc gcgaagatca tctttcagat cacggttttt caggatcagg
    10321 ttgatcacca gaaagatcag aagaaccgaa agagccacca gagcgtagat cacttttatg
    10381 gaaaaatccg catctgattt gatcttgtct actttctgga gcacggcatt tgccgcatca
    10441 gaaacggctt ctgtctccgt ttctgtttct gtctgtgcct cttttgcttt ctcggttgct
    10501 gcctccgtct gtgcatccgt ctcgttcgct gctgcgatct gttctgccgc atttggacgc
    10561 tccgcctgca cattcagggt atacgttgcc tgcataccgc tctcggatgt taccgttatg
    10621 taaaccgttg tctttccgtc cgtcagagtc tgaccggaaa cttcacaggt cgctgcaccg
    10681 tcggaagtca ccggacttag ctgcagttca gtcgtgcctt ccggaacctc cacctcatac
    10741 tccgttgtgc tgtactcaaa atccggagaa atactcgtcg cattctcgat gccaagatcc
    10801 gaaagggaat tgtcatcgga accacttgca aacacagtca ctccggaaat acagagcagg
    10861 agcatcatgc acactgccca gattttccag gtctttttcc acatcatgtt ttctcccttc
    10921 aaaaacgttt atgcttcgtc aatcgctttt ccaatatcat gacgataata tttattctca
    10981 aaatcaatcc actctaccgc tgcataggca tttgcacggg cctctttgag gtcttttccc
    11041 tttgcagtca caccgaggac acggccgccg tttgtcacga tgccttcttc cgtgcgtttg
    11101 cttccggcgt ggaatacata gtaaccatct ttatctttga atttgtcaag accacggatt
    11161 acgaaacctt tgtcgtattt caccggataa ccgtcggatg ccagtacgac gcagactgcc
    11221 gcattgtcct caaactgcag gtcgatctgg tcaagtgttc cgtcaatgca cgcctcaaaa
    11281 acgtcaatga tatcggtttt catacgcggc aggacaacct gtgcttccgg atcaccgaat
    11341 cttgcattgt actccaggac acgaggacct ttttcggtca gcatcagtcc gaagaagata
    11401 atgcctttga actctcttcc ttctgctgcc atcgcatcga ccgttgcctg atagatatat
    11461 tttttacaga atgcatccac ttcttccgta tagaacggac ttggtgagaa agttcccata
    11521 ccaccggtgt tcagtccggt atcgccgtct ccggcacgtt tgtgatcctg tgcggaagtc
    11581 atcgtcttga tggtcttgcc gtccacataa gaaagtacag acacttctct tccagtcatg
    11641 aactcttcta ccaccattgt attgccggct gtaccgaatt tcttgtcctc catgatggtt
    11701 tttacgccgt cttttgcttc ttccagcgta ttgcagatca gcacaccttt tccgagtgcc
    11761 agtccgtctg ctttcagaac aatcgggaaa tctgcttttt ccagatatgc aagtgctgcg
    11821 gatgcatcgg taaatgtttc gtaagcagcg gtcggaatat tatatttttt catcaggtct
    11881 tttgaaaatg ccttggagcc ttccaggatt gctgcattct ttctcggtcc gaatacacgc
    11941 agtccttctg cctcaaatgc atctacgata ccgccaacca gaggatcatc cataccaact
    12001 accgtaagat cgattgcctg ttcttttgca aatgcgacca gtttgtcaaa ctccattgcc
    12061 tggatcggca cacattctgc aaattcttca attccggcat tgcccggtgc acagtaaatt
    12121 ttatctactt ttggactctg tgcgactttc catgcgatcg catgctcacg tccgccgctt
    12181 ccgactatca atactttcat cctgcgtcct tctttctata tataaagtac ttgaatatac
    12241 aatgtctccg aatcttttcg catttctgag atcctgacat cgtctctatt ctgtgattat
    12301 cgttttgcca ttttccacac ggacttttcc ttctgcgatc aggcggatcg cctgcggcat
    12361 gattttccat tctgcctgct ccatgacacg ccgctgcaga atttccgggg tatcgtcctg
    12421 ctgtacttcc actgccttct gaatgatgat cggaccggta tctgttcctt tgtccacaaa
    12481 atgcaccgtt gcaccggtca cttttacacc gcgcttaagc acgccttcgt gtactttcag
    12541 cccgtaatat ccagttccgc aaaatgccgg gatcagcgat ggatggatgt tgatgatctt
    12601 gtttggataa gcatccacca tgatctccgg gatcacgacc agacatcctg ccagaacaat
    12661 gagatccggc tcgcaggact ggattttctc cagaagtgcc tggttaaatg ctgcccggtc
    12721 tgcaaaatct ttcggtgaga cacatgcagc tgcaatgtta tgatcctttg ctctttgcag
    12781 ggcataggca tttttattgt tgctgatcac ggctttgatc tcggtatttg gaatgcttcc
    12841 gtccgcaatg ccgtcaatga tcgcctgaag attggttccg cctccggaca ccagtactac
    12901 cagctttagc ataaggtcac acccttctca ccgttctcaa tctcaccgat cacgtaagcg
    12961 gtttctcctg ctgcttcgat cgctttcata gtcttgtcaa cgtctgtcgg atcaactgca
    13021 acaaccatac caatacccat gttgtatgta ttgtacatca tctgctcttc gatctggcct
    13081 tctcttgcca gcatcttgaa gattgccgga acctcatagc tgtctttgcg gatcacggca
    13141 cgtactcctt ccggaagcat acgcggaacg ttctcataaa atccgcctcc tgtgatatgg
    13201 ctgcatcctt tcacacgaac gcctgcttct ttgatgctgc gaagtgcttt cacataaatc
    13261 ttggtcggtg cgatcagagc ctcacccaga gtctttccaa gctcttcgtg gtaagtgttc
    13321 agtgtttttt catccatgct gaatactttt cttaccagtg aaaatccgtt gctgtgtaca
    13381 ccggaagaag caataccgat cagaacgtct ttctctttta aatcttttcc tgtaatcagg
    13441 tctttttcat ctactacacc aactgcaaaa ccagccaggt cgtactcatc taccggatag
    13501 aatcccggca tctcagcagt ctctccgccg atcagtgccg catcagactg tacgcagcct
    13561 tctgctacac cgcttacgat ctgagcgatc ttttccggat agttcttgcc gcaggcaatg
    13621 taatccagga aaaacagcgg ttctccgcct gcacaggcta tatcatttac acacatggca
    13681 acgcagtcga tgccgacggt atcatgttta tcaaggataa atgccagttt cagtttcgta
    13741 ccaactccgt ccgtaccgga aaccagtgtc ggtttttcca tgtctttgaa tttttccatg
    13801 gaaaatgcac cggaaaatcc gccgatattg gtcagtactt cacttctcat ggtcttcttg
    13861 atgtgctctt tcattaattc aactgatttg tatcctgcct cgatatccac tccggctttc
    13921 ttgtaatcca tgctnnnnnn nnnnattggg aagattgctg cacacatgcc cggcagatga
    13981 gctgccatac ctgctccggc aataatcact ttaaagcctt tgctctctgc tgtctttgca
    14041 tattcaaaga aaacatccgg ttcgcgatgt gccgagatga tcgtcatctc ataatcaatg
    14101 cccagtttct ctaaaacgtc tgcagctttt gccataaccg gcatatctga gtcgcttccc
    14161 attacaattc caacctttgc catggttcaa tctccttttc gcatcattgt aagtgtttaa
    14221 catcttttta attttactaa tactttcttt tattgtcaac ctgcaaacct taattgagga
    14281 agcgattcag ctcctccgtg cccggaagta cgaactttcc gtccgcaaag atcaggattc
    14341 ttctgccgtc agcacactgt acttcgatgc tgccaagctc gtcatatggt atcgtgatat
    14401 caagatgatg tccaaaatat gcctctcctg gatcttcttt tctgcgaatg gaaatctcat
    14461 tgtctcttgc gatgatctcc tttttgtccg gattgtatac cggtgtatct tctgcccagc
    14521 tgtagcaggt atctcctacg gcaaaatgcg gtccggtctt ctcagcgatc aggatcggca
    14581 gttttccttc aatatgatat tcccttgcca tagcataggc tgctgtgttt gttccgatcg
    14641 caaattcgcc gattggcaga gtctcatgat tcgccagcac attttcccgg acatagttct
    14701 gattgtactc ttcctgctca aaattcttgc aggtatatgc ttttaccata ccatcttcaa
    14761 attccagatg cagtttttca tactttaagc cttccagata aaccgtacat gcctcaagaa
    14821 tgccgcttgt tcccttcaat atcggggaag taaagacttc tccgaccgga atgttgacat
    14881 ctgccacaca attttcgaaa ttagtctgct tctttggatc attcagtttg tgcagggcaa
    14941 tgtgcatatc ggtgtgattt tctccttttc cgagcacatg tacactctca ccctgatcca
    15001 gggcatcgat cagtttctgc tggatatctg catagagctg atagtcaagc gtattaatgc
    15061 gaatcgtctc tttaaaaatc tccgggaagt ctgccccgat ttccgggacc ggaaaggcga
    15121 tgatcgtaaa actgcgttct ttgccgatga tatagcggtt tgtgatctgc acggattcgt
    15181 tatccatctg tgtctgcagt tttttctgtt tatccgacaa ataataggct tccggttttg
    15241 tctgcggata aaactccggc tccccgaagg tatcaatgca ggccgggcct gcatgaccgt
    15301 ttgccagtgt tttataattt tcgtacgctg aacgcatcac gcccaggcgt cgctccataa
    15361 aggccttgtc cagatagatt gcctggtctg cccggtgatc atagtcaaac tggcggttcg
    15421 ggattgcccc gtaatagccg atgcggtgca cacctttttt attgatacgc tccagtgccg
    15481 cacgatagat gaccggcttt aatcccattt tttcaaaatt tcgtattgcc agacgcacca
    15541 tccgctcaaa tccgaccata tagcggatgt tgaccgtctg ttttttcgaa agatccttgc
    15601 cgccaagcac aaatccgatc cggtatcctt cggtaaacgt agatgccatc ttttctacag
    15661 tctcttcatc caggctgttc ataaattctg ccatcttccg ctcgttttcg gtgatatact
    15721 caccatagcg atacagatag cgaaggtctt tcagatccga ctccatcacg atcttcgctg
    15781 caaaatcaag ctttggatca acagcttccc gaatgcggtg tgccaccgtg atctcactgt
    15841 aatcactctc aaaccagtag atgatctgct ggatccttcg gtatcccggg agctcttcct
    15901 cctcaaagca gttatacact tcgatcagaa gttcctgaag gatcaccttt tcatgcagcc
    15961 gctgctcaaa agcatagccg atcagggcat acaattcggc atacagaaaa cttaagatgc
    16021 gtccgtgcac ttctccgagt ttttccaccg catactttgg atttccatag ctgtgctcat
    16081 agttctccgg aagaatgtcc tcataaaggc tctgattaaa tgcctgccaa tgctcaagcg
    16141 tatagctttc cgtttttcca tcttttattt ttcggtaaac ttcatccatt tttataacaa
    16201 aagaagccgt ttttctgaaa taatccccaa acggctcctg tgcggtatgc tcttttgtta
    16261 tggatgcaag tcgttccatt gagagagcgt ctctctcttc cagtactgtt ctctccatct
    16321 ttccaccgcc aatcttttaa aggttacagt aaagtaatnn nnnnnnnnag ttgttccata
    16381 cttcgtactc ccgcaattcg tccctggcat ttccttaaat cccaattaaa actacaaata
    16441 cccacaatgc caccatgatg ccgccgagga tcgacccaat cttcggaaac aggtagcgca
    16501 cattgttttc gccaaatccc atcagtccga acacgacact gctgacggca aataccatgc
    16561 caagcagccc gatgctgccc aggtaaacgc cgccctgtcc gtccagataa tatgccagcc
    16621 atgccagaac ggcaaaaatg acaagtgaga gtgtaccaag tatcgttgaa agaatacctt
    16681 tgccggaatg tttctctttt gcaaaagaat acatacgctt tttagccata tatctttctc
    16741 cttatcttat tacatatcca aaacagcaga tacccgatca caccgcccag ggtgttgagg
    16801 atcaggtcat ccacatcaaa gcttcccacc ttccagacaa gctgtatcgt ctccacgcac
    16861 agactgaact cgaagctaag aagcaggatc cgtaaaactt tccccgtttt cggccatatg
    16921 accggcagga tcatcccaaa cggaatgaag ccgatcacat tccccgccag attgcagaat
    16981 actgcgaacg tccccagcat ctcccggtga acccagaatc tttttatttc ccgaaagggc
    17041 tgaaggttgt aggcatacat tcttccttcc acggatcttc cgtatccttc cgcaaaaaac
    17101 agaaaatacg tcagtgccag cagataaaga aaaaacagca ccgctcccag tatccgcacc
    17161 ttcttttttg tttccttcct cataaaacta aatcaatacc tgacttttac acttcagaag
    17221 tcttgcaccg tttacgatca tcatcacgcc ggctgaaatt ccggttacca gaaccacgat
    17281 cccgactgcg agacttgccg caccggtacc tcttattgtc ttaaaaattt tttcgttcat
    17341 gaaaagcctc cattttaccg aattattttg ccccgtattt ctcataatat gcatcgatcg
    17401 ctgcttttaa tgtatcatcg atcacaactt ttccgtcaca ttctctgtta tagagatgct
    17461 ctacgacatc ctgcatattt acgattgctg cggtctcaaa accgtaaaga tctttcactt
    17521 catccagagc acactttacg ccgccttttc cgacttccat acggtcaaga gaaaccatca
    17581 gtccgacgat cgtcacatct gcggcacctt ttaccttcgg aaccgtctct tccatggatt
    17641 tgccggaagt agtaacgtct tcgatcataa tcacacggtc accgtctttt aatttgcttc
    17701 caagaaagct tcccttgtct gcaccgtgat ctttttcttc tttgcggtcg gagcagtaac
    17761 gcacttcttt tccgtaaagt tcactgtatg cgatcgcagt cacaacacta agcgggattc
    17821 ctttgtaagc cggtccgaac agtacgtcaa aatcatcgcc gtatcggtca tggattgctt
    17881 ttgcataata ttcacccagt ttctttaact gagaaccggt cacataagct ccggcattca
    17941 taaagaacgg agattttctt ccgcttttca gtgtgaattc gccgaatttc agcacatcac
    18001 aatctaccat aaattcaata aattcctgtt tatactgttc catttcttat ctccttactg
    18061 cacggcaccg accagttctc tgatgtcgga tacattctgt gctctcatat atgcttcgat
    18121 cccttctgct actttgaggg tggtcatcgg atcgataaag tttgcggttc ctacagaaac
    18181 ggcagttgct cctgccaaaa tgaactccag ggcatcctct gcattcatga tgccgcccat
    18241 gccgatgatc ggaagtttta ctgcctgtgc ggtctgatat accatacgca cggcaaccgg
    18301 tttgactgcc ggaccggaca taccgccggt cttgtttgcc aggacaaatt tcctgcgatt
    18361 aatatcaatc ttcataccgg tcagtgtatt gatcagagaa agtgcatctg caccgcctgc
    18421 ttctgctgcc cttgctgttt ccgtgatatc ggtcacattt gggctgagct tcatgataac
    18481 cggctgtttt gccacacgtt tgatctcttt tgtgatgtcc tcgacagact ttggattctg
    18541 tccgaaagca atgccgcctt ctttgacgtt cgggcaggaa atgttgatct ccagaagatc
    18601 cactggttcg tctgccagac gttctaccac ttcacagtag tccttcgtac ttcttccaca
    18661 gacattgacc acgatcttgg tatcatactg tttcaaaaac ggaatatcac gttttacaaa
    18721 tacatcaatg cccggattct gcagtccgat agcatttaac atgccgccgt agacttctgc
    18781 cacacgcgga gtcggattgc ccggccacgg tacgtttgcc acgccctttg tgaccactgc
    18841 accaagcttt gacaggtcca caaattcact gtattctgct ccggagccaa aagttccgga
    18901 tgctgtcata accggatttt tcagttcaac tccggcaata tttacgcttg tacttatctt
    18961 tgtactcaca gttccacctc cgttgccaga aataccggtc cgtctttgca gacacgtttg
    19021 ttatgtacat gagaatgatc gtccacctct tttgactggc agacgcaggc aaggcaggca
    19081 ccgatgccgc atgccatacg ctcttccatg gaaatccagc actcgatatc ctgttcctgt
    19141 gcataagctt tcagtgcacg gagcatcgga gtcggtccgc aggcatagat cacatctgcg
    19201 gtaagtttat tctcacggat cgcatcaagg acattgccct ttgtgccgac acttccatcc
    19261 tctgttgccg cataaaatac tgcatttcgc tcaagttctt cttttaaaaa cagtttgtca
    19321 tcacggtatc cggcgatcgc tgtcttttca cccggaagct gcctggaaag ctcaaccata
    19381 ggcggaatac cgatgcctcc gccgatcaga aatacctttt tgccttcggc acgttccatc
    19441 ggaaatccat ttccgagcgg tcctaaaatt tcaagcttat cacctttctg acactgtgca
    19501 aactccgccg ttccttttcc tgccacacga taaacgatac gaagtgcctt tttatcctta
    19561 tcaatctcac acagactgat cggacgcggc agaagtctgc tgccatcgtt gctgtacacg
    19621 gaaataaact gtcccggtac agcctgctct gcgatcttgt ctgtctgaag ccacatactg
    19681 taaatatcag gagcaatgca ctcctgcttt aaaacttctg caagttcttt atatttgcca
    19741 gccatattaa aatcctcatt tcttttcttt ttgtcctgtc aaaccaggcg gctgtatgaa
    19801 atctgcaaat gccaatcccc acattttgaa aacggctgct tattttgccc tctcaagagc
    19861 agttccgata tctgcgatca ttgcctcaac agctgctctg gatgcctccg caaaatgttc
    19921 ttcaccgtat tttgcatact gctcctgttt gtaagcagca atgattcctc tggatgagtt
    19981 tacgatcgca ccaagtccgt cttcgttgaa gaagtgtacc aggtctgcac cttttccgcc
    20041 ctgtgcacca taacccggta ccaggataaa ggacttcggc atgatcttac gcagcacttt
    20101 gccctgttcc gggtaagttg ctccgacaac ggctcccaca tagctgtaat catcacccat
    20161 gcagtcctcg ccccattttg caacctgctc accaaccagc tcgtagagcg gacggccgtc
    20221 gatcagacga tcctgaaact caccgcttga cgggttggaa gtcttgacaa ggatgaacag
    20281 accttttttc tcctctttgc agacatctac aaacggtttc acaccgtcgg ttccaagata
    20341 tggatttacg gtaacaaaat cttcgtcaaa tcctgcaaag ctgttgtttc cgactttgac
    20401 ttttccaaga tgtccgactg cataagctgc ggatgtcgat ccgatatcgc ctcgtttgat
    20461 atcgccgatc acaacaagat ctttggattt acagtaatct acggtctttt taaaggccat
    20521 aagaccagga ataccgaact gttcgtacat ggcgatctgt ggttttactg ccgggatcag
    20581 gtcatacaca tggtctacga tctctttgtt atactgccag atggcttctg ctgctccttc
    20641 cagtgtctca ccgtgttctt caaatgcacg tttctggata tgagccggta cataggaaag
    20701 catcggatcg agtcctacta cgatcggtgc atgtgttttc tggatcttct ggatcaattt
    20761 ctgaatcata atcttatcct ttcttctgtg gatccacaca tttgctgtgt ggaacggcat
    20821 ttatcattct atttatcttt ataaacgacc tttccgtcgc agatcgtcac tttgactttg
    20881 cctcgtacgg ttctgccgtg gaacggtgtg ttgcgtcctt tggatgcgaa ggtattttta
    20941 tcgatcacat actcttcgtt cggatcaatg atgaccacat cggcgatctt gccttctgca
    21001 agggaacctt tgtccagtcc caggattttt gccggattgt agctcatctt ttctgccatc
    21061 tgcatcggtg taaggtactc tttttctacc agctcgctga tggtaagcgg aacggctgtc
    21121 tcaagtccga caataccaaa cggtgctgcc gtaaaggaac gtctcttttc ctcttcgctg
    21181 tgcggtgcat ggtcggttgc gatcacatcc ataatgtcat ctcgcagacc tcgttttaat
    21241 gcctctacgt cctttttggt acgcagcggc gggttcattt tatagtttgc atcgctggca
    21301 tcgacatctt tggaggtcag cgtaaaatga tgcgggcata cttctgccgt tacatggatg
    21361 tcctgttttt ttgcctgttt gacgatctcc acgcttccat aagtagagca gtggcacaga
    21421 tgtaggtgtg ctcctgtctc tcctgccagc atcacatcac gcacggcgat gatgtcctcg
    21481 actgcattgc tgatgccgcg aagtccctgt ttgcggatcg attcatcgtc atttgccaca
    21541 ccgccgtcca ccaggtcgat gtcctcgcag tgtgccatga ccggaacgcc gcactctgct
    21601 gcgatcttca ttgcttcttt gcagagcagg gaatccataa cggatttacc gtcctcactg
    21661 attgccggag ctccttccgc taccatggat tcgatatcgg caagttcttt tcccttctgt
    21721 ccttttgtga tcgcaccggc ctgcagtacg tgaatcggtg ccatcgcttt tgccttgttg
    21781 tgtacataac ggatattaag tctatcatct gttaccggct ttgtattcgg catcgccagc
    21841 acggtggtaa atcctccgtg tgccgctgca gctgctcctg tctcaaccgt ctccttgtac
    21901 gtctgtcccg gatccctcaa gtgaacatgc aggtcgatca ggcccggcat aacgtagcag
    21961 ccttctgcat cgatcacttc ttctgcctct tccttgatct cctggtctac ctttacaatc
    22021 ttatcctcct cgatcaggat atcataccgg ccatcttttc tggtgtccgg atcaatcact
    22081 ctgccgtttt ttatcagtat actcatcttt tggattcctt tctgtaaaac atcccaaggc
    22141 atgccatgca cacccagcat tttcttctat cattctacca taccagagga aatgccgcaa
    22201 accttttttc gtctccttga tttttacaaa tgcagatgct atgataagtt ttaaagaaca
    22261 gctgccggat tttgccggca gataccacgg aggtagcttt atgactgtaa aaccgccaga
    22321 ttttggtgga aaaccgtacc gctcgctgga ttatcatctg cgggaccgct acggtgaaaa
    22381 gatttacaaa gtctctttag acggcggcat gacctgtccg aaccgcgacg gcacgctcgg
    22441 aaagtgcggc tgtattttct gcagtgaggg cggaagcggt gattttgccg gtgacaggac
    22501 acacccgatc catcagcaga ttgaggaaca gaaggcccgc ctttccccga aatttccggc
    22561 aacgcatttt atcgcctatt ttcaggctta caccaatact tacgctccgc tttcatatct
    22621 ggagcgtatt tttacagagg cgattgaaca cgaagacatc gtcggacttt cgatcggcac
    22681 aagaccggac tgcctgccgg acgagacgct ggatctgtta tcggagctga ataaaaagaa
    22741 accggtcacg gtagagcttg ggcttcagac gatccatgaa cgaacagccg cttttatccg
    22801 cagaggctat aaactttcct gttttgaaga tgcacttgaa aaactgcagg aacgtaaatt
    22861 agagacggtc gttcatacga ttcttggtct tccgggcgaa aatacggaaa atatcctaga
    22921 gaccatgcgt tacttaaatg cacaccacat tgacggcatc aagctgcagc tgctccatgt
    22981 gctgaaacac accgatctgg cagcttatta tgggcagacc ggatttcaca ttttatcgga
    23041 agatgaatac gttgatcttg tgatccgctg tctggaagtg ctctcgccgg atatcacgat
    23101 ccaccgcctg accggggacg gtccgagcga tctgctgatc gcaccgctct ggagcctgaa
    23161 aaaacgcagc gtcctcaacc atatccacca cgaactgaaa gttcgtgata cctggcaggg
    23221 acgtctgttt accgaatgaa agtaaagcgg acacagttga gacctgacac gcttttgcag
    23281 agatttttta cataaggagg cattttatgg ctgaaccatt tacgctttat aaattaatta
    23341 tcttatatat gcttgaccgc gtggattttc cgcttacaaa cagccagatt tccagttttc
    23401 tgcttgaccg cggctacaca aactatttca cgctgcagca agctttttca gaactggaag
    23461 aagccaatct tgttaagcca cagaccatcc gcaataccac acagtttcag ctgacaaaga
    23521 gcggacgcga ggcacttggc tatttctgtg accgcatccc gaatgcaatc caagaggaag
    23581 ccgatcagta tcttcaggaa cacaagattg agctgcgaaa tgaggtttcg gtcacggcgg
    23641 attattaccg taccacggca aaagaatatg ccgtacactg tgtcataaaa gaaaaagagt
    23701 ccgatctgat cgacctgact cttacggttc cggacaaaaa acaggcagaa gctatgtgca
    23761 agtcctggaa aaacaaatgc gaaatggtct accagtttct gatgacacag ctctcctgaa
    23821 cacctggcag aattgaagtt cgaagtacag aaccattctg ccctgcattt tattcttcat
    23881 gcagccagtc cagatgcttc ctgatctgct gctcatatcc gctgtcactt ggctcataaa
    23941 acttcacttc gccgagttca tacggcagat aacgctgctt tgagaaatgt ttcgggaaat
    24001 cgtgggcata ttcataacca atgcccctcc ccagcttccc tgctccgcca taatgtgcat
    24061 cctgcagata cggcggcacg gaagcttttt tatttttcac cgattccatt gccgaaaaga
    24121 ttgcattcgt tgctgcattg ctcttcggag cacatgccac atagattgct gcctgagaaa
    24181 ggatcagctg cgactccggc ataccgacac gctcgacagc ctgtgccgcc gcaactgcca
    24241 cctgaagtgc ctgcggatct gcattgccga catcttcgga tgcacagatc atgatccttc
    24301 tggcgataaa ggtcacactt tctccggcat agagcattct tgccagataa taaactgccg
    24361 catccggatc tgatccccgc atactcttga taaaagcaga gatcgtatcg tagtgattat
    24421 ctcccgcctt atcgtagcgg atcacacgtt tctggataca ctctgatgcc acgtcgatcg
    24481 taaagtagat tttcccgtct gcagaacgct ccgttgtcag cacaccaagc tctacagcat
    24541 tgagtgcaga cctggcatcg ccgtctgccg cttctgccag aaaatctgcc gcttcctcat
    24601 ccatgcagac atcatatgcc cccataccgc gttcgctgtc cgtgaccgca cgcaaaatca
    24661 gttttttgat atcttctttt tcaagcggtt tcagttcaaa aactttggat ctggaaagca
    24721 aagcaccgtt gacttcaaaa taaggattct ccgttgtcgc cccgatcagc accactgtcc
    24781 cgtcctcaac aaacggaagt aaatagtcct gctgcccttt gttaaaacga tggatctcat
    24841 ctacaaacag aatcgtcctc tttccataca tgcccatcct ctgttttgcc tcggcaacga
    24901 cttcttccat atctttttta cctgccaccg tagcattgat ctgcgtaaaa gccgcactcg
    24961 ttgtgccggc gatcactttt gcgatcgttg tctttccggn nnnnnnnnnc agcgctctgg
    25021 caatggatcg ggcaatggaa gtctttccgg ttcccggcgg tccgtaaaaa ataacggaac
    25081 tgagtttatc tgctttgatc gcccggtaaa gcagtttatc ttttgccaga atatgctgtt
    25141 gtccgacaat ctcatccaat gtcctgggcc gtatcctaga agcaagcggt gcttcactct
    25201 ccattttcgt ttctctcata taatcaaata aatccaaagt tcttcctccc ctcctgtgtt
    25261 tctttttcgt ttgaaagata ccgcacaagt aactccgtca caaagtaact ttctataaca
    25321 tcctgtcatt ccagtacaag cttatctgct gttacaaatg tatagccctg ctcctttaac
    25381 agatccacga tccgaagtgc cgcttccacc gaagcttcat agcggtcatg cagcaggata
    25441 atggcatttt cttttgcctc agacatcact ttttctacga tctgatccgc attttctgtt
    25501 ttccagtcca gcggatcgat ggtccagagc accggaaaca tcccaaaacc gcactccagg
    25561 tttttgttcc atgccccaaa aggaggtctg atatattcgg tgtcatagcc gacaatgctt
    25621 ctgaccagct cgctcgtttt tgtaatctct tcacatgcct gtttatccga catttttgta
    25681 acgtccacat gggaccaggt atggtttccg atcagatgtc catcttctgc catctgcttt
    25741 accaattctt catttccttc gatattttcc ccgatcagaa aaaaggttgc ctttacgcct
    25801 cgttttcgca gaccgtccag caactttttc gtatagaccg gatgtggtcc gtcgtcgaaa
    25861 gtcagggcaa tctcaggcgg aatatccgct gatgccggaa gtgatctttc acaccagcgg
    25921 atatattcca cactgccgat tccgattccg gcaaacagta caaacagata gatgtgaaaa
    25981 agcatgtttt tccagtttct gtataaaaat cctttcaccg gaatttcgcc ctttccgttt
    26041 ctttcatgct atcctatgtc tgccggaatc cttctatgct atgttttatg atcttacttt
    26101 gcctgccgca atgcttcctt ttgccagcag cttgctgtaa gctttctttt tgttcttcgg
    26161 cacttttacg gttacttttt tcgaaatgcc cttaaatgcc gccttttcta ctttcttcag
    26221 agattttact ttcttcaggc tgatgttctt taacttttta tcgcctttga atgcattttt
    26281 gctgatggtc tgcagtttgc tgccgttcac ggtaactttc gtcaagtttt tgcagttttt
    26341 aaatgcactt ctgccaatct ttttcacatt gcctccgatg agcacggaag tcatcttttt
    26401 attgccctta aaggcgtttg ccgcaatacc ggtcactttg taagtcttac catttgcttt
    26461 gatcgatgca gaaattttta cggctttctt tgttttgccc tttgctgcca tgctctttac
    26521 ttccacggtg tcatttccgg tgatcgtgta gttgaacact ccggctgcat ctgcgatcac
    26581 atctccgatc tttccggcag aaccagaagt gttctggtcg gatttattgt cctttggttt
    26641 ttcggtctga tccgg
//
LOCUS       DAWDWH010000009        23357 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_1310_length_23357_cov_14.608403, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000009 DAWDWH010000000
VERSION     DAWDWH010000009.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 23357)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 23357)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23357
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1310_length_23357_cov_14.608403"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5703..5712
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7433..7442
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15274..15283
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18393..18402
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7045 a         4843 c         5926 g         5503 t
ORIGIN      
        1 cgaccgaagc ggagcgtaga aaggattgtg ggagtgcaac gcacggaaca atccgcaggt
       61 atcataattt aagaaacatt atgggcaagc agcaaagcgg attgcgaata tccgcttaaa
      121 tacataaggg agggtttgaa gattatggac aacaaattat atgaaatgat ggactgggca
      181 gcagtagagg ggattgtata ttcagaagaa gatcatccgc acgatattct gggggcacat
      241 gtgacagaag aagggattct gatccagacg tttctgccga cggcaaagag tgtgacagtg
      301 gtgttgaaag acagcaaaaa agaatatccg atggatttag aggatgagga aggctttttc
      361 gctgcactgc ttccgggtaa aaaaattcca aaatatcatt atcacgtaga ttttggagaa
      421 aaagcagacg gaaaagaaaa tatcgtagaa tacgaagatc catatcgttt tgcaccacag
      481 atcacggaaa aagaaacgaa gaaatttaat gcaggcatct gctacgatat ctacgaaaaa
      541 ctgggatcac atccaatgac ggtcaatggt gtggaaggtg ttttgtttgc agtatgggca
      601 ccaaatgcca tgcgtgtcag cgtggtaggc gatttcaacc tctgggatgg cagacgtctg
      661 ccgatgagaa gactgtggga ttccggtatt tttgaactgt ttgtaccggg acttgctgca
      721 ggacagctct ataaatatga aatcaaggca aagggagggc tgacattctt aaaagcagat
      781 ccgtatgcca atgctgcaca gcttcgtcct gacacggcat cggtgatcac agacctgagt
      841 cagtatgcat ggaaagatga agcatggctg aaacagagaa aaaatgcaga cagtaagaag
      901 gctccgatgt ccatttatga ggtacatctt ggctcattcc gcaagccgga ggacggcagg
      961 gagttttaca attaccgtga actggctgtg atgcttgcgg actatgtaaa agagatgggc
     1021 tatacgcata ttgagctgat gccggttatg gagcacccgt ttgacggttc ctggggctat
     1081 caggtgaccg gatactatgc accgaccagt cgttacggca caccggaaga tttcatgtat
     1141 ttcatggact atatgcatgc acagggcatc ggcgtgattc tggactgggt accggcacat
     1201 tttccgcgtg atacgtttgg actggctgcc tttgacggaa cctgcctgta cgagcacctc
     1261 gacccgagaa aaggttccca tccgcactgg ggcacactga tctacaatta cggtcgtccg
     1321 gaagtcaaga atttcctgat cgcaaacgca ctgttctggg cagaaaagta tcacattgat
     1381 ggtatccgta tggatgcggt ggcttccatg ctctatcttg attatggaaa aaacgaagga
     1441 gaatgggtgc cgaatatcta cggcggaaac gagaatctgg aagcagtcga atttttccat
     1501 catctcaatt ccatctttaa acagaaggag ccgggggttc tgatgatcgc cgaagaatcc
     1561 acagcatggc cgaaagtaac gggagccgtt gaggatgagg gtcttggctt tgactacaaa
     1621 tggaacatgg gctggatgaa tgacttcatt tattatatgt cactggatcc gatcttccgt
     1681 accgcaaatc atggggcgct gacgttcagc atggtttatg cgtacagtga agattatatc
     1741 ctgaccttgt cacatgacga ggttgtacac ggcaaagcat ccatgatcgg caagatgccg
     1801 ggtgaccgtg agcagcagtt tgcaaactta cgtgccgctt atggatttat gctggcacat
     1861 ccgggcaaaa agcttttgtt catgggacag gattttgcac agtatgatga gtgggcagag
     1921 gatcggtcac tggagtggga gcttttgcag tatgatgagc acaagcagat gcagaattat
     1981 gtgaaagcac tcaatacatt ttacaaagag catccggcac tttacgaaat ggactatgat
     2041 ccggatggat ttacctggat caataatatt tctgccaatg aaaatatggt agtctttacc
     2101 agaaacacaa agaaaaaaga agagacgctg ctgatcgtct gcaatttctc accgctgact
     2161 tacgaaaacc acaagatcgg cgttccatac cctggaaaat acaaagaaat ctttaacagt
     2221 gacagcaaag aattcggagg cagcggagtc ggtaatccga gactgaaaca gtcaaaaaaa
     2281 tcagaatgcg atgacagagc ggattcgatc ctgatcacgg ttccgccgat gagcattacg
     2341 gttttttctt atacaaaagc ggcagccgga agagcggtca acaaaaaaac ggcaacaaag
     2401 aaaacagccg agaaaaaagt taccgaaaag aaaacagccg aaaagaaagt ggctgctgta
     2461 aaagaaacgg aaaagaaaac agctgagaag aaaagcagtg aaaaagtaac ttcgatcaaa
     2521 gccgcttctg agaaaaaagc agctgcaaag aaaacaaccg caaaacaagg aaccggtaag
     2581 caggcaaccc caaaagcaga gctggagatg gcagctgcaa gtgaagaaaa agtgacaaag
     2641 acggcagaaa aagcaccgaa aaaaagcctc aaagaagaac tggaagccaa aatcgcagaa
     2701 agtgcaaagg cagaggaaga aagaaaaaac aagacaacgg gcagaaaacc ggccgcaaag
     2761 agcagacgaa acaagcagca ggagaaatag cagatggaac aaatagcaga gaatacggca
     2821 gattcgctga ccgaaagtgt aaaagaagtc aatcagttta cacagtattt tcaggggaaa
     2881 atgccggggc ttatggatct gggcatcaaa ctggtcattg tcctgatcgc attttttgtc
     2941 tgccgcaaga tcattcagct gatcctgaag atctttggcc ggtcctttga acgagccggg
     3001 atggaggcag catccgcaca cttcacactt tccattatga aagccatcat gtacgtgatg
     3061 gtggtggcag tcatggccac ttttctcgga gtgtccggag cgtcggttgc tgcactgatc
     3121 ggctcgatgg gagtcggcgt tgtgctggcg ttaaaagaat cgctttcgaa tattgcgggg
     3181 ggatttatct tattgtttat gaaaccattt gtcagcggag attatatcca cgaagacacc
     3241 catggaaatg aaggaaccgt ggtgaaggtg gatctctttt atacgacact tctgacgatc
     3301 gataaccgta ccgtatcgat tccaaacgga atcatcacca acagcagtct gacgaacatt
     3361 tcaaagcagg ataagcggca gctgcgtgaa aaggtaggga tttcctatcg aaacgatatc
     3421 aatgaggcaa ggaaagtgct gctcgctatt ttgcagaagg atgcggccat tctgaaagaa
     3481 gagaagatcg aagtggtcgt tgaaagtctt ggagactccg gtgtgatcct cgggtggcat
     3541 gcctgggtaa gaccgcagga ttattttccg gcaaaatggc ggatcaccga agaaatcaaa
     3601 tacagatttg atgaaaatca gattgagatc ccatatcagc agattgatgt gaagatcagc
     3661 cggtaagaat atccggtaaa agcaggagga gtacagagtt tcctgcctgt gtcggtcaat
     3721 gtagaaaaat gacaatgaca gagggagaaa aacagaaata aggggcaagt tacaggagga
     3781 taaaatttta tggaaacaaa gaaaaacggt gtgcaggcgg aagctcatac aaaagagcag
     3841 acttccggcg gacatgccca cagtcacacg catgtgctgg aggacggaac agttgtcacg
     3901 catacgcata cgcacagtca cgaacataca aaagcagtgc taaaccgtct ctcccgtgca
     3961 atcggacacc tggagtccat caagaaaatg gtggagaacg gcagagactg cagcgaagtg
     4021 ctgatccagc tttccgcagt caaagcggcg atcaacaata ccggaaaagt catccttcag
     4081 gaccatatcc agcattgcct ggtagatgcg atcgagtccg gagatatgaa ggaaatcgaa
     4141 gaattgaata aggcaattga caggttcatt aaatagtgta taataattca gtagactttt
     4201 taacgatgaa tttcattaga atctgctgca gaagatgcgg ccgaagagga aagcatgaag
     4261 aaagacaaaa cacgcaagaa aagttctgca tataaaaaat atcgcagaca caagatttta
     4321 tttatcacag aactgattat tttgattccg ctgctggtat ttggcttttt gtatgtacag
     4381 ttcgaaaaga gactgggaaa tattgatacc gtgaacattc agtccggtga tatccaggtg
     4441 aacgaagaag tggagaacaa tgcagttctg catggctata ccaacattgc cctgttcggt
     4501 gttgattccc gtgacggaag tatggattat acaaacaccg atacgatcat cattgcgagc
     4561 atcaataacg acacgaaagt ggtaaaattg gtatccatat accgtgatac ctacctgaat
     4621 atcggtgaag accgttatgg aaaagccaat gcagcctatg cgaacggcgg ttataaatgg
     4681 gcgatcaata tgctgaacca gaacctggat ctggacatca cggattttgt aacggtgaat
     4741 atgaatgcac tggtagaagt agtagatgca cttggcggtt ttgattttga tctgacagac
     4801 gaagaggttg tacatatgaa caactactgt gtcggaactt ctgaaatcac aggaaaatct
     4861 tatgaacgta tcgagccgga agtggcgggc acgtatcatc tgaacggtgt gcaggcagta
     4921 tcctacagcc gtatccgtta tacagccgga aacgattaca aacgtaccga acgccagaga
     4981 gagctgatcg ccaaaatcgt ggcaaaagca aaaaaagcag atatgaagac gctggtagat
     5041 atcatggaca atgtgttccc gaagatctcc accagtctca gcaaatcgga tattgtatct
     5101 ctgggaatga atatgttgtc ttatcagctg ggggatacct gcggattccc gtttgaacac
     5161 cgtacaagtg atctgagcgg agattccgaa gttccggtta ccttatcctg gaatgttacc
     5221 agactgcatg actacctgtt tggcatcaaa gattatgagc cgtccgcagg tgtacaggag
     5281 cgaagtgctc atatcgaaga agtcagcgga ttcagcaaag gcagtgccgt cagcaaagaa
     5341 aattatacgc tgaacggaga agaagccgga aaagatgtcg atgacaatat tccgggaatc
     5401 caggagaccg agtctgagac agaaacccag acgcaggaag cggcagtcag cgataatggt
     5461 gacggcagtg tttatgatga taattcttac gatcaggagc agtataacga ttacagcccg
     5521 gatggatata attacgatga agaatattga gtcgagcaga tatctgcaat ggaaatggga
     5581 aatgtatttg acttagttaa attgtaatta tttttgtgaa aatacttgca aaacagacaa
     5641 taatcatgta tattaatttg agtcagcagc aatactgctg accgatgctg gactagctca
     5701 gtnnnnnnnn nngctcagtc ggtagagcac ttcactcgta atgaaggggt cgagggttca
     5761 agtccctttt ccagcttgac agaaaaagcc cgaaaatacg gcaaaaacaa aagaaagagc
     5821 atcctgtaaa agggtgctct tttttataat tgactaagat ttgactaaca tttttttata
     5881 catgtatata agaatcgaaa cgattaaact ggctgttatc agaatataga aagcgagcga
     5941 taacatgaaa atattaatca caggtgctca tggttttgtg ggcaatcgtc tgatgcagga
     6001 tctggatgat acggttgcgg caccgtccat gcgaggtatg agcgaagaac agatccgaaa
     6061 catgattgag aaaagcagag cagacgttat catccacaca gcagcaatct cagatgtcgg
     6121 gacgtgtgaa aaagatccga gggcatctta tgaagcaaat gtgcgtctgc cgttgtttct
     6181 tgcaaaagca gcgaaagaac aaaaattgat ctgtttcagt tcggatcagg tataccgcgg
     6241 atgtgcaggt gaaggtccat ataaggagga agatgccagt ccctgtaatg tttacggcag
     6301 gcataagctg gagatggaac agaaggttct ggatgtaaag ccggatgcag tgatgctgcg
     6361 ggcagagtgg atgtatgatt atccgacaaa gcggtcgaac tatcttcgta tgctgttaaa
     6421 cgaaaagcag gttctgacgt ttggaaaaca gtaccggggg atcacttatc ttcgtgaagt
     6481 tgcggaaaat atgccgaaga tttttatgct tccgggaggc gtttacaatt ttggaagtga
     6541 aacgaagcag acgatgtatg atatcacctg tgaaatgctg cacttcctga aaagtgacag
     6601 gaaaccggaa gaagtgccgc cggcacataa tctgtggatg gactgtacaa aagcaaagga
     6661 gagaggaatc cttttcagtg atgcgaagga agggctgaaa cgatgcctga aagattatca
     6721 tttagcaaaa gacagggaat aatagaaccg gggatttctc attgtgtgag aaatccccgg
     6781 tcttttatac agaatcaatc agaaataagc atacttcctg agatgacctg cagatagttc
     6841 atgttttgtg caaatctttt acaatgcgat ggaatgtccg ctttcataaa cctgcccatc
     6901 gacagaaagg taaatatccc aggtcttgcc ttccaagcca accggggaaa cccatccgga
     6961 aacggtaact tttgttgcat cctcaccggc agtgccatac tgggtcagtg cgtaagcttt
     7021 tgtctcatct ccgcttacta agatcacgta agcatcttcc agtgcatcgg aagctttgtc
     7081 tgtagtgtaa gcacctttta aggagaattt cgcatcatcc agggtgaatg cccatccttc
     7141 cggaagtgct gcggcatttt caagatctgc cgtgtaatct gcggtggcag tttcgccgag
     7201 tgttccaagc agctcgcctt ttgcatttac atcaagtgtg gctccttcgg tatagagcgg
     7261 caggcggaga gaacggtaag tcagggatgc ataagtatcg ccggagatca ggatctcgta
     7321 agcaagtgtg ccgtttgata cctggtcgat atgggtgcgt ttgatcagtc cctgcttcat
     7381 catgtcggtc ggatagtgat catagcggtt gttttcttca tcatagaggt tgnnnnnnnn
     7441 nntggcaccg gctgtgatcc agagctggtc ttttgtgcca tccagggaaa ttacaccgga
     7501 gatccagtca gaataccact gcggaccacg ttcttttcca tactcaaaga cctgctcgat
     7561 cgtcatatca tcggtgttga tatggtaaac gacggctctg gagtagatat catcaccgga
     7621 aacacggttg tcattatcgc ttaattttac ttttgcagta ccattgtcaa acatcataat
     7681 atcaccgtta tcaagcatag tgatctgatg ctgtgcatac tgccattcaa aatcatcacc
     7741 ggtcggggtg aagaaatatt ttttatctac gccattccag ttggcaggat caccgaggat
     7801 ccaggcaagg gatttgtccg atttgtggat ggcgatgatc gcatctttat ggcgtgcaga
     7861 aagcagaacc agatcatttt tctcatcgta ccacagagaa ttattatgga accagtcaat
     7921 ctcatcggaa ccgtcagttg caatcgaagc ggattgtccg tcttctttgc caatcaaatc
     7981 agctgcgttt aattcccaga caacctcgcc gctttcacgg tcgatctcca caacataatc
     8041 ttctacggtg gaaagatccg gactgtctcc ggcaaccagc agattgccgt ccggcagttc
     8101 ctggaaatcg tggtgcatac cgcccgggat catgtactgg cggtaaatct tgccgctcag
     8161 atcgatttcc tgaagacctg ctttgtagta catactcttc agaagaaaag aagtcggcat
     8221 catcagatga ccattcttca gctgatgaac gccaagtact ccgccatctg tgaagtacca
     8281 gcggatgtca ccggcactgt ccagtgcgta gagcataccg gcagaagagc agacaaaggt
     8341 cagattggaa taatcatagg cagaatcatc actcatggtt gtctcgatcg tgccgacgtt
     8401 gatatcagtt ttttcggtgg taacttctac ggttgtggaa gtgccatcct ccagtgtcag
     8461 gactacttct gttgtagcgc cattatacag cccgtagatc gggacaagat gatccgttgc
     8521 agcttcgaaa gttccggtaa tgtcattttt ttcatctttt cctttgacgg tgaccgtgcc
     8581 gccaagctct ttatctgtgt ggaaggcagc gacagcagtc agcggagaat tgccgtaagg
     8641 attgacgatc acatttggtt cttcaaaaga atatccggcg gatgcctcct gctgcagggc
     8701 ttcatcaatg ctgttctgct gctcaaacag atccgttttt tcaagagaag caaaggaagc
     8761 ggaagcagtc gttgcagatt cactttcaga agatgtttcg gaagctgttt ccgaagaaat
     8821 gctttctgtt tcactgttgt ttgctgtttc ggaagtcgaa ccggcggaag aacagccggc
     8881 aagcagagaa acagtcagag tacagatgca aagacgggat aaaagcactt tgtttttcat
     8941 aaaaacactc cttgtatcgt gatatttgcg gcaaaaatgc catagagatc atagtttcag
     9001 gttcttgttt gaacttccga aaaacagtta ctgaagatcc ggtaattcac aggaataagt
     9061 ttcggattca aacgggtaag ataaggtaag accagaagca tcttccttga tctcgaaagc
     9121 gatccagcct tcgttggaac caaagttcag cttggtacct gccatacgtg tcatgccgag
     9181 gttggtcaga aattcatcgt cgatacgggt gtattcattt ccatcgctgt cggtcagttt
     9241 cagggaagcc cagtcgatag cttccgtgct gccatctttg ttgatctgca ttttgatcag
     9301 ataataaacg tttccgtcag aagcttcctt cacataatca tttgtctcaa cgccggtgta
     9361 tccaaggtca acgctgacgt tttccagaga tttgttcttt tccagttttt cgacaacgaa
     9421 ctgccatccg gcaaccgtgg tatcggagat atcagcggca gcttcggttg ctgtcacatt
     9481 ttcagaagat gcctcggtat cagcagcctc agattcagca gctgtctcgg aatggtcagc
     9541 agtatccgca gcagtttcag attctgcggc actttcagtt gcaacgactg aatcggcagc
     9601 agaacgggaa gaagcggttg taccggcgtt gtcaagattt gagcatccgg tcagtgcaga
     9661 cgcacagaac agtgtggcac acagtagcat agcaattttt ttcttcataa taaaagacct
     9721 catttcttat taattaataa atttcggaaa ctatttacag ggcatctgtt gcccccgaca
     9781 tcatggaaaa tctgctcatg atgctgcggg cagaagatgc cgtaaaggat taggaatccg
     9841 cagatatttt aacacaaagc ataagaaaat accacgttat cataagataa aaaagtgaga
     9901 aaaaaatgga gaaatctcag ttgtttacca gtaaagagtg acaaacacag caaaaagcga
     9961 caaacaaaag ataaagaaaa tgaaaaaaat ataaagtcgt tgacagatac cgggcatggt
    10021 gctatattct cgttgaacaa atgttgaacg aaaagttaca ggaggtgtga aaatgagcag
    10081 tcaggggaca aaaaatcaga gacaggagca ggcggcggca accagacaga aactgctcac
    10141 atcggcacaa catctgtttg cacaaaatgg ttataaagga acgacggtcc gtatgatcaa
    10201 ccgcagtgtg gatctggcgg acggacttct gtatcattat tttccaggtg ggaaaaaaga
    10261 aattttcaaa gaagtcatca ccggcaatat gcaggaaatg tttcaggagc ttcaggagcc
    10321 ggaaaagcag gatgtatacg aaaaactgcc gctggatgcg gcattggatg cagcctatat
    10381 caattttatg acggtgatcg atgagcattt ggaatttatc cgcatgatgg tgcgtgagaa
    10441 cggtgtcagg gaatttctgt cagaagaaga ggtcagaagc atctggaaga accggattcc
    10501 gtggctgccg acacttctga aaagaaagat ggaagccggg gaagtcaaag agatggattt
    10561 tgagatggca gccctttccg taaattctat tctgatgcat tatgtgatga aacgcgttac
    10621 cgataccgga cacagtacac tggatgacaa ggcctggaga aagcggctgg tcgattatca
    10681 ggtaagtgtc tggtctgtgg aaaaacagcc ggaaaatgaa aaagagcaag aataaaaaaa
    10741 taccggaaaa aatctggaag ataaagaaga aagaaaggtg aatcatatgt ggcatttgtt
    10801 gaaagatatg cgaaagaagg acaaaggact tgcgtttctt gccgttcttc tgatcgcagg
    10861 tcaggtgttt ctggatctga aactgccgga ttatacaaaa gaaatcaccg ttctgatcag
    10921 ttctgagagc aatacgatct cagattatct gttatcaggc gggaagatgc tggcatgtgc
    10981 actcggaagt gccctgctgg cggtaattgt cggttatctg gctgcaaaga ttgcagcaga
    11041 tttcagtcac aatgtcagag gaaaagtatt ccataaggta tttgattttg gggcgggcga
    11101 gatcagcaaa ttttctacct caagcttgat cacacgtacg acaaacgata tcacgcagat
    11161 ccagatgctc gtagccatgg gactgcaggt gatgatcaaa gcaccgatca tggcagcctg
    11221 ggcgatcgtg aagattgtcg gcaagagctg gcagttatcc gttctgacgg catcagcagt
    11281 cgtgctgatc atggtcgtga tcggaaccct gatggcagtg ttgcttccga aattcaagac
    11341 tgtacagaaa caggtcgatg acgtcaaccg tgtgacccgc gagaatctga ccggactgaa
    11401 agtggttcgt gcattcaatg ccgaaaaata tcaggaagac aaattcgaaa gcgtcaacga
    11461 aaatctgatg aaaacgcaga tgttcaccat gcgtggcatg gcacttctgt tcccggtgat
    11521 gatttttgta cagagtgcat tatctcttgg tatttactgg ctgggagcca aactggttga
    11581 tgacatttcg atcccgttaa acggaacaat gacagaaata ttcacggcca tcggttcaag
    11641 ggcggatatg cttggtgatg tagtggcatt taattcctat gccctgtatg tagttatggc
    11701 atttgtgctg ctgcttatga tttttgtcat gctgccaaga gcacaggtat ctgcaggccg
    11761 tattaatgaa gtgctgaact ccgatatcgc agtcagagaa ggaaaagatg acggcagaca
    11821 gacggaagaa aaaggcagca tcacattcaa cgatgtgtcc ttccgctatc cgggagccgc
    11881 agaaaactgt ctggaacaca tcagtttcca ggtaaaccag ggcgagacac ttgccatcat
    11941 cggagcgacc ggttccggaa aatcaacgct tgcaggtctg gcagcaagac tttacgatgc
    12001 cagcgagggc gaagtgctga ttgatggcaa aaatgtaaaa gattacagtt tccgcggact
    12061 gtatgacaag atgggatatg ttacacagaa agcagtcctg ttctccgata cgatcgaagg
    12121 aaacgttgcg tttggtgagg cggcacaagc gatcaccgaa gaggatgtaa agaaagccat
    12181 cgatatctcg caggcacagg aatttattga taaaatggaa gaaggtcttt acagccatat
    12241 cgcacagagc ggcggcaatg tttccggagg tcagaaacag cgactttcga tcgcccgtgc
    12301 gatcgccaga aatccggaaa tcctgatttt tgatgattcg ttttcagccc tggattataa
    12361 aacagataaa gaactgcgaa gaagactttc caccgacctt aaaggtacca cctgcgtgat
    12421 cgtggcacag cgtatcggaa cgatccgcca cgccgacaag atcatcgtac tcgatgacgg
    12481 aaagatggca ggtatcggaa cccatgaaga actgatgaaa aactgtgaag tttatcagga
    12541 aattgcttta tctcagcttt caaaagcgga acttgcgtaa aggagatgcg gaatcatgca
    12601 taaaaaaaga caaaaaccaa agaatctgaa agccgcattg agcaatctgt tctcttactg
    12661 caaaaaagaa gcaggtatca ttttatttgc actgatcatg gcggcattag gtgcagttct
    12721 gacgatcgca ggaccgaatc agatcagtaa gatcacagat tatatatata acggactgac
    12781 cggcgggatt tccaccgaag cagtccagga agacatccag aaacagatcc agtccggaca
    12841 gatcaacatc atggactatc tgccggaggg aaccgatccg tcaagtattg attttcagaa
    12901 tctcgacctg acggacaaaa actccgagct tgcacagaac atcgtgaaca acatccgttt
    12961 aagcgatgag ttccggacag cccatcagga tgacggcatc gatatgaacg gcattttcaa
    13021 agtggcagtt tttcttctcg gactgtatct gatcagtgcc ctgtgcagtt tctcacagca
    13081 ctttatcatg gcaacggtga cacagaaact ttccaagcgt atgcgaaaag atattgacgg
    13141 aaagatcaac cgcctgccac tgaagtattt ctcagaacat tcatatgggg atgtgctctc
    13201 ccgtgtgacg aacgatgtgg acacgatcgg tcaggcattt tctaacagcc ttgcagcgat
    13261 cgtatcagca gtggcacagt ttatcggctg tctgatcatg atgtactaca ccaactggat
    13321 cctcggaacg accactgttg tgaccacact gctcggtctg gtgctgatgg tgctaatcat
    13381 gaaacactcc cagcggtact tcaccgaccg acagagaagc ctgggtgagc tgaacggcta
    13441 catcgaagaa atgtatgccg gtcatgatgt gatccgtatt tccggagcag agggacaagt
    13501 cagagaacgt ttcgaaaaat taaacggaga tgtcagaacg gcaaactaca aatcacagtt
    13561 cttatccgga ctgatgcagc cgctgatgac gtttatcgga aacttcggct atgtggcagt
    13621 ctgcatcatc ggtgccctgc tcgttatgaa aggtcagatc acctttggcg tgatcacggc
    13681 atttctgatc tatgtgcgtc tgtttgaaca gccgctgcgt cagatctccc agggtatgac
    13741 aaacatccag tccgcggcag ccgcttccga gcgagttttt gagttccttt ccgaggaaga
    13801 gctggaagat gaatccgcaa agatggcaaa tgtagaagaa gtagagggcg atgtggtatt
    13861 ctcccatgtg aaattttcct acccgagcgt gccggacaaa gagatcatcc acgatttctc
    13921 cgttcacgta aaaccgggac agaaagcagc catcgtcggt ccgaccggtg ccggaaaaac
    13981 gacacttgtc aacctgctga tgcgattctt tgagccaacc ggcggtacca ttacgattga
    14041 cggtgtggac acaaaagata tgacaagaga aaatgtccac agaaaattcg gcatggtgct
    14101 ccaggacacc tggctgttcg agggcagtgt gcgtgagaat cttctatata ataatgaaaa
    14161 tgtatccgaa gaaacgatga taaatgcctg caaggcgtgt ggcatccaca gcttcatcaa
    14221 agcactgccg cagggctatg ataccattct gacagacaat acttcgattt cagccggaca
    14281 gaaacagctg ctcacgatcg caagggcaat gatccagaac agcccgatgc tgatccttga
    14341 tgaggcaacc tcaagcgtcg atacgagaac ggagcttctg acccagaggg cgatggatca
    14401 gctgactcag aaacgaacca gttttgtgat cgcacaccgc ctgtccacga tcaagaatgc
    14461 ggatatcatc ctggtcatgc gtgacggtga cattgtagag cagggaaatc atgaagaact
    14521 tctggcaaaa ggcggattct atgcagatct gtataccagc cagtttgaac aaagtgaggt
    14581 acaggcataa tgagaggaaa aacgatcgta attgacagag aatacggaag cggcggccgg
    14641 gaggtcgcaa agcttctctc agagaaactt ggaatcccgt tttatgacgg tgaactgttg
    14701 gtacttgcca gcgaaaaata cggcattgac cttggcatga tgaaagatta cgacgagaaa
    14761 cgtatgggaa gcatcctgta cagtattgcc atggcgacca gctatgccag cgattttgac
    14821 aagatcacaa aaccatatga gatctacgag gctcaggcaa aagtcatgcg tcagctggtg
    14881 tccgaaggac cgtgcatttt tcttggaaga tgtgcagctg aaattttgaa agacaaaaca
    14941 gagcttttaa atgtctttat ctacagtagc gaccagcatg caaaaacaga acgcatcata
    15001 agagtggatg gtgttgcaca gagcaatgcg gtcacggaat tacataaaag agatgttcag
    15061 cggggcaatt attatcatta tttttcaaaa aaaacctggg gtgaccgtaa tcattatgac
    15121 ctttgcctga ataccgcaga attaggttat gagaaagcgg cagagatcct ggcagctgcc
    15181 gcaaaataag acctcattta tcagtgacgg gatatggtct tgcatctgat atggctttca
    15241 acggatgaaa gaggttccag atgagaaatt gttnnnnnnn nnnttacatt actctgcttt
    15301 ttgtatataa tatggctgta agttcatact aacaagaaaa gaaaaggagg caataaaatt
    15361 atgaaaattt tgttttttgg tgcgaaaagt tatgacataa aatcttttga tgcgtatctg
    15421 gacaaagaag aatacaaaga tctggatgtt gtatacattg agccgaatct tactcctcag
    15481 acagcaatgc tggctgccgg ttatgaagcc gtgtgtgcat tcgtaaatat ggacttgagt
    15541 gcggagacaa tcgatatttt aaatacatgc aaggtaaaac tgatcctgat gcgttgtgca
    15601 ggcttcaaca acgtagatct tgagagagca aaaggctacg gcatgaccgt aacacgcgta
    15661 ccgagctact caccggaagc cgttgcagag catgcgatgg cactggctct gacggcaaac
    15721 cgccacacgc acaaagcgta catcaaagta cgtgagaaca actacagtct gaacggactg
    15781 atgggtgtca acctctacaa aaagaccgca ggtatcgtcg gaaccggaaa aatcggtgct
    15841 gcgatggcac gtatctgcca cggatttggt atgaaagtga tcggctacga tatgtacccg
    15901 aacaaagatc tggatttcgt agaatatgtt gatctggata cgttgctttc acagtctgac
    15961 ctgatctccc ttcactgccc gttgatggaa gagacacatc acatgatcaa tatcgacacg
    16021 atccagaaaa tgaaagacgg cgtgatcttg gtcaacacct caagaggcgg actgatcaag
    16081 acagatgacc tgatccaggg aatccgcgag aacaaattct ttgccgttgg tctggatgtg
    16141 tacgaagaag agaacggttc cgtttacgaa gacatgtccg agacgatcct ggagcactca
    16201 accgttgcaa gactgttatc cttcccgaat gttatggtga catctcatca ggcattcttt
    16261 accgaagaag cactggcagc gatcagtgaa gtgacactgg ataatgccat gagttatctg
    16321 aaaggcacac cgaacggaaa cgaactgtaa ggtgcgtttg ctgaaagcgg atgacagaaa
    16381 caatatgaaa tagaatgata taaaataaga agaaagagga atttttgaag aagagttcca
    16441 ctttcttctt atttttgttc aggcagaata cacgtgaaag ccagtatttt gtatacagag
    16501 tacagaatcc ggtaaaaaga gatgtgatcc gaaaaatctg atgaaaaagg gagcatctgg
    16561 aaaaatattt cactggatta tagcaggaaa gcatgatata cttacagaca gtgcagttat
    16621 ttcgcacagt gaaaagcagt atctgtgaaa ggctgcagca taaaatcaaa aacaaggagg
    16681 atccaaatgg gagattttaa agaatggtta tcggataacc tgcgttattt cctggtcggt
    16741 gttgtcgtga tactgatcat cggcggttca atcctgggac tgaagattta ttcttcaaag
    16801 gtcaatggaa acagtgatcc ggctgtgatc cgtaaaaata cagaggcaca gacagagacc
    16861 ggaaaagaaa ctgagacaaa gaaacagact gaaaaagaaa cgaaaaagca gacagagaaa
    16921 gaaacgaaaa aacagaccga aaaagaaacc aaaaaacaga cggaaaaagc gacagaaaag
    16981 cagaccgaaa gttcaacatc ttccaatgca ggtacagaca gtaaaggaag tacagcagat
    17041 aacagccaaa atggcagcaa ttccgcaaac aatgcttcac agaacagtgg cggcacatac
    17101 agtaatggct cacagagcag cgacggtaca tatgataatg caggcaatga tgcaacgacc
    17161 gatggcagtg actacagcaa tgacacgcag gatgtgacaa cgccgcagac ggatgctgcc
    17221 cagccggttt acatgacaat caattccgca tgctacatgc gttcttaccc ggactacggt
    17281 gacaacatca tcggtcagtt tgaggcaggt acgaccgtag agttcttagg agatgagggc
    17341 ggatggtaca aggtttccgt aaatggccag gtgggctata tgggagccag attttttaac
    17401 tgacagacgt tgacaaagca gcatggctga ggtatgataa caagaagaca gagaggtcgg
    17461 attctggact tctctgtcct tttttatcat atttaaaatt taaaatgtaa aggaaaactg
    17521 tcacggcagg tttcctggat tgaaagggag agcggaacaa tgaagaaaaa agcattagca
    17581 gcagtacttg tagcagcagt aacgatgtct atggcaggat gcggaagcaa gcaggcagca
    17641 tccacagacg gcggaagcaa aacagaaagt gaagcaggaa gcgatgcggc agcagcggat
    17701 tccggggaaa agaaaaccct gcgtgtcggc atggaatgtg cctatgcacc gtacaactgg
    17761 acacagagcg atgattccaa cggagccgtt ccgatttcag gttcttctga ttatgcatac
    17821 ggctatgacg ttatgatggc aaaacacatc tgtgaagagc ttggctggga tctggagatc
    17881 gtaaagatgg actgggacag ccttccgaca tcggtaagtt ccggagccat tgactgtgcg
    17941 atcgcaggac agtctattac cgcaaaacgt ctggaatccg tagatttcac agacccgtat
    18001 tattatgcat ccatcgtgac actggtaaaa aaggacggac cgtataaaga tgcaaaaggt
    18061 ctggcagact taaagggtgc aacggctact tctcagatca acaccgtatg gtatgatacc
    18121 tgccttgatc agatcgaaga tgtaaacaaa ctgccggctt atgaaaccac aggaacgatg
    18181 ctggtatctc tggaatccgg agcatgtgac ctgatctgta ccgaccagcc gactgcaatg
    18241 gcagcctgtg tggcttatcc ggatatggaa atgctggatt tctcctcttc ggatgacaat
    18301 tatcaggtat ctgacgaaga gatcaacatc ggtatctctg taaagaaagg caatcaggaa
    18361 ctggtggatg agatcaacag cgttctggaa aannnnnnnn nnaacacgtt tgggaggaaa
    18421 tgtattatga gcagtacttt gccaacagac tattgggcca gagtcgttta tctggtcaca
    18481 cactacagtg catccttcct tcgcggggca ggaagcaccc tactgatcgc actggtgggt
    18541 acctttgtcg gatgtctgat cggttttgcg gtcggcatcg tgcagaccat tccggtgtcc
    18601 aaacaggatt ccatcatcaa aagagccctg ttgtttatcg taaaactgat tctgaacatc
    18661 tacgtggaag tattccgcgg aacacctatg atcgtccagg cgatgtttat ctattttggt
    18721 gccatgcagt atctcggcat cactatggga atgtggttcg ctgcattctt tatcgtgtcg
    18781 atcaacaccg gagcctacat ggcagagacc gtgcgaggcg gtatcttatc gatcgatccg
    18841 ggacagaccg aaggtgcaaa agcaatcggt atgacgcact ttcagacgat gctgtacgtc
    18901 attttcccac agacgctgcg aaacatcatt ccgcagatcg gcaacaacct gatcatcaac
    18961 atcaaagact cctcggtact gtccgtaatc ggtgttatcg agctgtatta tgcggcaaaa
    19021 cagacagccg gagcttatta ctggtggttt gaggcaatga ctgtagcgat ggtcctctat
    19081 ctgatcatga cctttgtctt ttcaagactt ctgcgcttct gggagtccaa actggacgga
    19141 cctgcaagtt ttgatctggc aacgaccgat cagctggcac atacgagcgg aaattacagt
    19201 tatccgggag aaggaagctt tgacaaagag agaaatatcg aaactgatgt gaacggagga
    19261 agagacaaat gaatagagat gcgattctgg aagtaaaaca cctgtccaaa tcttttggtg
    19321 ccaacgccgt tctcaaagac attgattttg ttgtacatcc gggcgatgtg acatcgatca
    19381 tcggttcttc cggttccggc aaatccacac tgcttcgctg catcaacctt ctggagacac
    19441 cgacctccgg ggatgtggta tttcatggag acagcgtggt ggataaaaag gtcaatgcgg
    19501 cagcataccg tgcaaaagta ggcatggtat ttcagtcttt taatctgttc aacaatatgt
    19561 cggttcttga gaactgcatc atcggacaga caaaagttct gaaaagaaac aaagaagaag
    19621 caaaagaaaa ggccatgcag tatctgacaa aagtcggcat gaacccgtac atcaatgcga
    19681 agccgcgtca gctttccggc ggacagaaac agcgtgtggc gatcgcccgt gctctggcga
    19741 tggagccgga gatcctgctc tttgatgagc cgacatccgc actggacccg gaaatggtag
    19801 gtgaagtact ggaagttatg cgtggactgg caaacgaagg catgaccatg ctggtcgtta
    19861 cacacgagat ggcattcgca agagacgttt ccaaccatgt cgtttacatg gcggacggtg
    19921 tcatctgcga agaaggcagt ccttcggata tttttgaaaa tccgcagaat gcaaggacaa
    19981 aagatttcct ggccagattc cgtgcaaaat aaaaacagag acaaaaaaga gaaatataaa
    20041 aagaaaaacc agaaaaagct ctcaggcaat gtcataggga caaagccggg gagctttttg
    20101 tgtgtgtgca ggcggcaaag agatcagaac atgagctgga tcagcatttt tcataggcaa
    20161 gacgcggatc accgtttgca agatggatga tgccgcattt gcggaatccg tttttttcaa
    20221 gcacatgctg catgatcttg ttgtcgtggt gtgtgtcgat gcgaagatgg gaagtctgcg
    20281 gctcgcagaa atccagcacg tttcgcagaa atccgtgcac ctgcccgtca cttgcgacca
    20341 tgtggatcac gccgtaaggg gcatcggata accagctgcc ttctgagatc tgtgcataag
    20401 tcggatcgtt ttcgatggca aaataaaaac agccgcggat ctgcgaaaaa tcatcgggat
    20461 ccgtgaagac aaacagcttc tgtgcgagga tacattcccg gagcagatca gcggaaggat
    20521 gtccgtctgc ccactgggtc gggttcccgg tttttgccat aaaagcccgg gcagttgcat
    20581 aaatattcag gatgacagga aagtcatcca taacagcttt tcgtaccata aaaaacctcc
    20641 aaagtataaa aaatccacaa aaaaaattgc agacggatgc attcgatttt gtaaacattc
    20701 aaaatccgca tctttcctgc ggaaataatt ataaatgata gcatggaaac agaagaatgt
    20761 aaagagagca caggcactct gctgccgatt ttgagaaata tgcaacaagc cttgtatcgc
    20821 aggaaaaaat aaagtataat gcttaaatgc ggaagaatga cgcaccgttt ctacaagtgg
    20881 atgtctctcc gactaaaatg ataaaaagga tgataacatg gacagcagag agtatctgaa
    20941 ctatgtttta gataaattgc aggagcgtat tcgtaaactg gatgatgcga tcctgcacgg
    21001 acagaaagaa atcgaaaata tgcatgagta ctactgggaa aactacacgg agatggacca
    21061 gtatggctac gaagattttg ataatcagca ggcactgctt ggtcaggtca atgccaacaa
    21121 ggagaagctg gatcttcgcc ggagattcaa aaagatgctg gattccccgt ttttcgggcg
    21181 ggtggatttc atctacgacg gcgaagaaga ggcagaaacg ttttatatcg gcatcggcaa
    21241 tttttcaaaa gaatccggaa gactcccgct ggtctatgac tggcgtgccc cggtttccag
    21301 cctgttttat gactacgaca aaggagaagc ttactatgat gccccggcag gacgcatgga
    21361 gggcgaagta ctttccaaat ggcagtacaa gatccgtggc gggaagatga tttatgcatt
    21421 tgagagtgat gtcaagatcg atgatgaggt tttaaagcag gagcttggca gccacgggga
    21481 tgtcaaactg aaaaacatca tcaagacgat ccagaaagag cagaatgcga tcatccgcaa
    21541 cacacaggac cggattctgg cgattcaggg cgtggccgga agcggcaaaa cttccgtggc
    21601 actgcaccgc attgcttatc tgctctatca cgaccgggaa aatctcaagt cctcgaatgt
    21661 actggtactt tcgccaaacg gcgtattttc agactatatt tcccacattc tgccggagct
    21721 tggcgaggag aatatccggg agatgagttt tgatatgttt gcctacaggg agctccggga
    21781 tacggtcagc gactgtgaag accgctgtga ccagatcgaa aaagagcttc tcgatgaaaa
    21841 gtatgcagaa agctgccgca aaaaacagtc gatcgatttt gtattgcagc tcaatgagtt
    21901 tgtgctggga ctggaagacc gcctgatgcg tttttcagac ctgaaataca agggcatgac
    21961 aaagagcgaa cgtcagttga cggagatgtt ttattaccgc tttccggata ttccgctgct
    22021 ggagcgtatg caggctgtca tggattacat ggttgatgag tacgaaacgc tgatcggcag
    22081 agatctcggc gatgacgaga tcgagatcgt acgcggcaaa ttcatgaaaa tgtaccgcag
    22141 tacggatctg tacgtgttgt ataactggtt cctgaaagaa tacggctatg agacactgcc
    22201 gcaggtttct tatgagaaac gctttctgaa atatgaagat gtttatccga tgttgtatct
    22261 gaaatatctt ctgaaaagtc gcaggatgga ccgaaacatc cgtcatctgg tgatcgatga
    22321 gatgcaggat tattcctata tgcagtatct gatcctggat aagatgttct cctgcaagat
    22381 gacgatcctc ggagataaag cccagaccat ggaggagaaa acgagggatg tcttaagttt
    22441 cctgccgcgt gtttttggcc gcggcatccg aaagatcaac atgaacaaga gttaccgcaa
    22501 caccatggag atcgcatctt atgccaattc catttcagat gtatccgata tggagctgtt
    22561 tgagcgtcat ggaaaaccag tggaagagca gacattttca gacagaaagg cggcactgga
    22621 agccgttctt gagaaactgc gtctggaaga gtttgagacg gccgctgtga tcgagatgac
    22681 acaggagcgt gcaaagaaaa gtgccgctta tctgaaagag cggatggaag agcttggcat
    22741 ggatacagag aatcgttttt ctgtggtaga ccgtgacagc acccacttca aaaaggggct
    22801 gactgtcacg accttttatc ttgcaaaagg tctggaattt gaccaggtat ttgcacttta
    22861 tacgacgcag gacaatacgc cgctgcaccg gcaggccaga tacatctgtg cgacaagggc
    22921 actgcatgaa ttgtatatgt atgaagtaaa agcaagtcag gattaaagaa aggcaaaatc
    22981 aaataagttc agatgcaatc tggcgttctc gttctttttt tgagaacacg cagccgcagt
    23041 aatcctgccg gtacagacca taaagagctg agagctcgac ggaacgttta taaccatttt
    23101 tctttttgaa atcggaagga agccatgaga taccgtaagt atctgcaagc tcctctccga
    23161 tttcatttaa tttctgggcg ttttttaaag gactgatcga gagtgtcgtg cagaaatagt
    23221 caaatccatg ttcctttgca agctgtgctg ttttctccag acggagccga tagcactgaa
    23281 aacaacgttc gccgccttcc ggaatctttt ccagtccctt tgccatctca tagaatcgtt
    23341 ccttgtcaaa gtctccc
//
LOCUS       DAWDWH010000010        22820 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_1351_length_22820_cov_16.718515, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000010 DAWDWH010000000
VERSION     DAWDWH010000010.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 22820)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 22820)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22820
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1351_length_22820_cov_16.718515"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3551..3560
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20749..20758
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7582 a         3950 c         5507 g         5761 t
ORIGIN      
        1 attccttact ggctgtaagg gatattaacc ataaatatat tgtagtagaa agtatatgta
       61 gaaatggtaa ggcagattgt agaaagtgag gcgaagaatg atgaagacgc tgaatgatta
      121 tatggcaatg tcctatcgta tggaaattgt ggaagacaaa gatgaaggcg gatttgtagt
      181 ttcttatcca gatttgcctg gttgcattac ttgtggtgaa acagtggaga gtgcagtagc
      241 aaatgcactg gatgcaaaaa aaaaacatgg cttgaagcag cattagaaga aggggtagaa
      301 attcatgaac cagacagttt ggaagactat tctggacagt ttaaattgag aattccgaga
      361 agcctgcaca gatcactggc agaacattct cagagagaag gaatcagcat gaaccagtat
      421 tgtgtgtacc ttctttccag aaatgatgca gtgttctcaa aataaatgat gaaaattatt
      481 cttcgttggt tacacattac attcatgatt ttatggagaa atgacttatg ttgatgcctg
      541 gtatttttgg agaaaagaac tggaaatcga tctgtcggga ttcaagaaag aggaagttag
      601 ggcagagctg aaagatggtt atctgacagt cagtgcagtc aaagacctgg atgaggatga
      661 aaaggataaa gagaccggta agtacatccg ccgtgaacgt tatcatcaat actggcattt
      721 tatactgaaa tactaaccgt ttggctagtt ccatataaaa atcatgtatg ctataataaa
      781 gtcagagaat gcagatttta cgataagctg gcaaaggggc gaaaaagtat gaaaaaacga
      841 atgttgaaga taatgagtat gggagttacg gcgtgtctgt tgcttggaac aacaccgaca
      901 ccgatgttga caaaggcagc agtaaccgaa acagcaaacc atatagcaaa taaaagcaaa
      961 gaatcggaaa agacagagac aaagaagagc aaagaatctg aagcggtaaa tacagagaaa
     1021 actaaagaaa cagaaatacc gggagcaaaa aaagagaaag aatccgaaac agtaaaatca
     1081 gagaaaagta aagagacgga aataaagaaa gcaaaagaat ctgagacaac agagacgaag
     1141 aaaaataagg aatccgagac aacagaaaca aaacagacga aagaaactgc agcagcagag
     1201 acaggaaaaa ttaaagaacc tgaaacagca aatacagaca aaaacaaagc atccgaaact
     1261 tcaggcacac agaaaaataa ggaatctgaa atttcagaaa cgaagaaaga aacagtaagc
     1321 aaaggaactg aaacacaaaa agtaacagaa gtacaaaaag aaactaaaat acaaaaagaa
     1381 acagaaacac agaagaaaaa cgaagcagca aagcagaaag cgaaaaacaa ggcagcagct
     1441 tctgcaaata agaaacaggg tattgcactg caaaatgcgg cggagataag cgttacgacg
     1501 caaaatgagc tgagggcagc tctgaaaaat gatgcagttc agacaatcaa cattgaagga
     1561 agttttactt atacatatga agtggaaaca gataagaaga ttgtcattaa gaatggggct
     1621 gttttcaaat ggagtgctta tcaggatacg ttccgtataa cggacatgct gattgagaac
     1681 ggagcggttt tccagatcag cccatttgat tttatgtcaa aagcggtggt agccggtacg
     1741 atcaccaatc agggtgacat tcaggtaacg tctgcaaggg gcgaatgttt ctttacagca
     1801 tcggtaaaag gaagcggaac gttttctcag acaacagaac aaacttatat cagctatgga
     1861 gccgtacagg atgctatgat taccggcaca ggctatcgga tcaatcttct gaaggatttg
     1921 tctgtaacac cgacagtcag tctgccggat tctatgcaga caggagatac gatcacaccg
     1981 attttcacca atatcataga tggcgtagac ctttccaaag catttactta taaatggaac
     2041 aatggaagtt atgatattta taatggtgcg tccagtccga tgctgacgaa atccggaaca
     2101 ttgaaactga cggtgtcccc gaaaaaacca tatattatgc ggaccagtac caacagttat
     2161 ccgggcagtt tggatgccag gggaactgtt cagaaaaagc agtatgatgt catttatgta
     2221 gatcctgcaa atggaaataa caacaatctg ggtgatacaa aagaaacttc catgaaaacc
     2281 ataaccggtg caatgaacaa tctcacagaa aacggaacga tcgtactgct cggtgattac
     2341 agcagttata cggtgatcga taaaaatgtg acgataaaaa gtgaggatgc taaacagtat
     2401 tcgtttgcac cgacattggc tctgaatgcc gataatcttc gtgtgacact ggattccgtg
     2461 aatctttcaa atctgacggc aacgggatac gggacaaacg aaacactgac cataaaaaac
     2521 agcagcggaa gtattcagtc taccgatcag aatattgcga atgttcaggt ggaagattcc
     2581 aacttaagcg ggagactgac agcatcacag aatctggaat ttaaaaatgt tacattttct
     2641 ggcaaatttc agacagaaaa attcattgct gacggtaaaa atatcctgat tccggagaaa
     2701 aacaggatca gccgcattga tggaacagcg acaatcacca atccgatcac gatacagacc
     2761 gagccggtaa aaggaaagaa agtactggaa atcagcgaga ataataaaga tacgattctg
     2821 tcaaaactta aactggcaga cactcagaat gataagttcc agatgaaagt ttcaaaacag
     2881 tacaatggca catatattac cattacgcag cgtgtgacat ccggcggaaa actgctgatt
     2941 gcaaatgagc cgtttatcgg tgaggcagta gaagattctt atgcaacaat gcgaaatgag
     3001 gactgttctg taaaaacagc agtatggagc ggatacagtc atgcggcagg atcaaaatgg
     3061 gcagccggtg atgtaccgga actgacgatc acgttgaagg tctggagcag cgacggaacg
     3121 ggcgtcgaga gcaaacattt tgatgagact ttccgtgcac aggatatcaa agtgtattcc
     3181 tggaaagatc tggacaccta tccggacacg gattcggata caaacctgaa taaggatgca
     3241 gagattttgg taaaagacgg acagggactc agtgcagacg gtgaaacctt tacgtttaca
     3301 ctgcgttatc cggccgtaga gcgtatggaa cagacgatca cgacagactg cagtgcacgt
     3361 agtgcgtatt gcacagagaa gcttgctgca cgcccggtgc aggcacaggg tacggtttcc
     3421 tatgaaagca gtgatccgga tattgcatcc gtagatgctg caacgggaga aattaccgca
     3481 aagaagcctg gaaaggtcaa aatcctcatc catgccgcac agacagatct ttataaggcc
     3541 gcaagtgcag nnnnnnnnnn acggtggatc atgctgcggt gactgcaccg actgccataa
     3601 ctggtttgaa atataacgga aaagcacaga aactggcagc accaggagaa actctggaag
     3661 gcaagatcat gtacaaggta aatgacggtg aatggaaaga ggaagtgccg actgccgtca
     3721 atgccggaac atatacagta tattataaag cggtaagagg agaaggacac ggagagactg
     3781 aagaacaaca ttttgacgta aatgtttctc agaaagaaat cggaatagaa tggacgaata
     3841 ccgaagttat ttatgatgga acggaaaaat taccgacagc agccgcaaaa ggtctggcag
     3901 aaggtgatac ctgtaatatt acagtaaccg gaggacagac ggaggcaggc acttatacag
     3961 cccaggcagc tgcgattgat aatgcaaatt ataagcttcc ggcagagaca accgttactt
     4021 ttgtcattcg gaaagcagat tttgaactga ctggcatgcc gcaggcaaaa acagatctgg
     4081 tgtatgacgg aaaagaacag gaactgatca cggccggttc tgcaaagagt ggcactttgc
     4141 tctacaaact gggcgatggc gagtggacag aaaaaatccc gtccgcagta catgcaggaa
     4201 cctatgatgt atcttataaa atcgcaggtg acagcaatca taatgatctg atcggtttgg
     4261 aagttctgca tatcaagatt gcggcaaaat caatcgcaga tgcagatgta aaactggcag
     4321 atgcattgaa atataccgga cagcagcaga aacaggaaat ctcaaaagtg acggcaggaa
     4381 atctggaagt tccaaaggat gattatgaag tgagcggaaa tcaggcagca gaagccggtg
     4441 tctatacgat gacgatcaca gcaaaagaag gaacaaattt caccggaagc agagaatgga
     4501 cttatgtagt tgcaccggtg aaaatgagcc agatcacaaa agataaagac ggtaaagtac
     4561 agatagggca tggtactttt tctgtgaaga ttgaacagga caaggacaca gcacaagcag
     4621 cacttgtaac aagcgaggca gaattcattg agaaactggt ggatgccggt gatattacag
     4681 cagatgagct gacacggaca gcaaacgggg catcgatgga actgctgctt cacattcaga
     4741 atgaatcttc gcttggtgaa gaatccagaa cgcagataca gaaaaaagca gcagaaaaag
     4801 gttatacacc gggaacctgc tttaaactca gtctgaaaaa gtatatgacc gaaaacggta
     4861 cgacagatca gggaactgtg atttcgcaga acggtaaaat aaagcttatt ctacagattc
     4921 cggaaagtct tcagaatcag aacagtaaga aagaccgtac ttattttatt ctgtgcagca
     4981 agaatggaaa tgtggaagca ttaaacggca cttatgatgc aaaagcaaaa accattacct
     5041 ttgaagcaga agattatgcc gattatgcgt tggtatatca ggagacagat aagcctgata
     5101 atggaggcgg aaatgatcag gaaccagaaa cggatgataa cacaaagaaa gataataata
     5161 agcagaacgg taataaagac aataacaaga acaacaacaa caataaaaac aataacaaaa
     5221 acaataataa caacaaccag aacaatccgc aaaataacaa tggaaatggc agtcagaagt
     5281 caggcatcac aactactgct tccggaactt cagcgacaac tgcgggcagc actgtaaagg
     5341 ccgcaaatac ggcggacagt gcgaatgttt tcggaaatat cctggcattt ctcggagcat
     5401 ggatgattct gcttggagcc tggatcagaa gaaaaacaac aaaataatct cagtgaaatt
     5461 taaagggaat ccctgccgca tgcagggcaa tgggatatac ttgcaagggt aaaatgtgta
     5521 taaaaaggag cctgaagcta tttgcttcag gctccttttg agccaaagtt tatgcgttgc
     5581 cgctgaccgt tacatttgca acgcctcccc agtcagttga tttttgagga tcttttgttt
     5641 cacgggaaca ggcatatact tgaatttatt aagcatatat gatatattga ttaaatcaac
     5701 tggatgtata tgtgaagtaa aacggatcag atgaaaaaat caaatgaaag aaggatgcat
     5761 atggaaatga aagatgaatt tttattcaaa acacacatgc tcgataaaaa cggagaaaag
     5821 accggagtaa atcagatcgc ggattatatg ttccgggcgg atatgatcta cagaatgaaa
     5881 cttgcatcgg acatgggact gccggtgctt accttaattg cacgggagct ggaagagaaa
     5941 tttgatgaaa attccagttt cccggtgaca gcaacgaaaa atgatccaaa tgcaccacta
     6001 tatcgccaga atgtcggaag gatcgccaag tttatcatgg ataagctggg gtatgtccag
     6061 gcgacaggga gtgtcaggct tccggcggtg tcaaagtcga gatatttttc gacaagtgcg
     6121 gtatatgaga aaaagaaaaa aggaagttat gattttaaaa taacagattt tgtgatacac
     6181 ttgcagaaaa caaaataaca gaaaaacgcc cagaaaactc cccctttttt cgaaatgtta
     6241 tttataaatt ataatagaag atggaaagag aataaaacat attttctttc agatcggtgg
     6301 ctttctgatg acgcagctcc gggaagtcac tgaaaagaat aacacgaaca gggtagaaac
     6361 ggaaaggcgg gaagtcatat gtcagaaaag atttttaagg tgtcagggga agaattccgg
     6421 aaacagtaca aggaagctta tgaaagcgga agagcatcac ttgtagataa aatgccggcg
     6481 gagttctttg cgagaactgc catttcagat gaggtggcag tactgctttt tcggtatatg
     6541 agctttctgg atgaggcaga caaaacaacc tgtcgttact tgcaggaagt aaaaaaactg
     6601 atcgaaaacg gaaagttcca gcatttgaaa gagtgtgaag gattgaatcc acaggtaatt
     6661 gacatgatcg atacattgta tgaaaaacgt ccaagagaat gctgtgatga actgctttgt
     6721 attgcaatga cgatgtaccg catttttaat atgcaggaaa gaccattgca gagggcggaa
     6781 tacaaaagac gtaccgaaat ggcctggaga gcagtgaaca aagccagaaa agataagctt
     6841 attgagcttt ctgaaaggaa aaaaagctta aaagagagtc cggcagacaa tattccactg
     6901 gcagtgaaag tgaaagaaca ttttgacagc agtggtatgc gtggtcagga agaggctaaa
     6961 aagatagtgg caatgagcgt gcataaattt gttattagtg aaggaaagga tcgaaatccg
     7021 attcttctgg aaggacctac cggttccgga aagaccttta tgtttcagct gcttgaggaa
     7081 atgccggaat tgaaagatct gtgctttttt aattattgtg cctctgatat gacaccgaat
     7141 ggcttcagcg gggatgacat ttccaatgtt ttctcaaaat atgaaagaat ggcaagacaa
     7201 aagatgaaga aaggtgagaa tggcttacag atgccgctgg gaattatttt tcttgacgaa
     7261 atggataaga tcatcggtat gtcaaacaca gacagtaatg gtgaagactg caatgccatt
     7321 gtgacagctc agctgctgac tgcgattgcc ggaacccaga actatggaag tgtcgatacc
     7381 agtaaaatcc tgttcatcat ggcaggtgct tttgaggagc ttgaaaacag cagaaagaaa
     7441 aagcgggaag aacacggtat tggttttgca gtaaatgcat ccgcaaagga gaaagagaac
     7501 agcatgaccg gatatgatct tcgggaagaa ctggaagaga tcggtgtttc aaagcagttt
     7561 ctcggaaggg tgccttacat tgtacatatg gaccggctgt caagagctca gctgcgtcag
     7621 attctggtgg ataaggaaca tggagtattt ggtaagaaac agaagtggtt caaaagaaac
     7681 ggaaaaacgt tgacttacga tgaggatctt gtggaggaga ttctggatcg tatggaagaa
     7741 cacgatatgg gagccagagg agcggctgca atgctggaga cattgattgg aacagcagaa
     7801 tacgatatgt tcgcaaacgg ttataagaat ttacatctgc acaaaggaat attcttcaga
     7861 gaggaacctg ttttcgatga acagaaaggt aagagtcgtg ataaatagta taaagaaaat
     7921 ttttctgatc attaaatggt tttttgtatg ggtttacaga aaaacagaac agttctgcaa
     7981 aagagtatgg agaagacagg atttactgtt atgtcttgca actttgacca tgatcggtta
     8041 tgctacattc tggagatatg acaatgagat ttttgatggg aaagaagtct ggtggatgat
     8101 atccgtgatc gccggtctgg ttgtggcact gatcatcggg atcgtcggtt accgtatttt
     8161 tttcagatgt gcaccgccgc tgtatcttgt gttttctgtg gtacatcttt tccagagaaa
     8221 accgatgatc gaaaaagaat tcgaggcaac actgagaaat ctgtttctga atgaccacag
     8281 ggcagaaagc acggttgaac ttgtactgat gcttctgaca gcatttgtgc tggcggcatt
     8341 tgaaagaaga aaccgggcac tgtttcgaaa agaaagagaa cagaaagaga gggaaaaaag
     8401 tttaaccgga agaagagcaa gaaggatgaa gaaaaagagt ccgacaaagc cggatgaagg
     8461 ctttttggga aagcattttc cgtgtatcat taccggggct atgatcctgg tgatgatgtt
     8521 ctcttatctg ttttccggat ttgtggtaag agttgtgtgt ataacagtgg ctgtttttac
     8581 gttgactgtt tatacagctc cgctggtccg gaaagtccgg gcggtgctct ttgccacggc
     8641 aggatttgtg tgcagcttcc atcatgaact gtcaatcctg aaatacaaca aagcgaacgg
     8701 gagctatttc ctgaagtgga tggcggagcg tggatcactg ccgaatatcc tgatctggga
     8761 aaaattcgga ctgatcggat actgtctggt tgtggcagca gtcctgagta ttatctggtt
     8821 tatctggaga gcatggagat atgcaaataa aaaaggtaat gtctgtgatg aagtgatttc
     8881 cattaccgct tttgtacatt ttgctctcgc aggaacctat tttgtggcag aaatggcatt
     8941 tccggatgga aattatttct ggggaaataa ccttccgttt tttacaggaa ctctggaaaa
     9001 taatcttttg tatctggcag agctggggat cgtgtatcgg atcctgagaa aaagtattgg
     9061 aaaatagcgg gcagatgccg gatgtctgaa aaggaggcag tgtatgaatc caatttgttt
     9121 gtcgattaag caggaagaaa ccggaaaaag aatcaaacag ttgttgcagg atcacggata
     9181 caaggtaaaa gatatccagg gggcgatggg atttgaaacg ccgcaggtgg tatacaaatg
     9241 gctgtcaggc aagtcgcttc caagcctgga caacttcgtg atcctgagca gattgctgaa
     9301 tacaagcatt gaagatatcc tcgtcattga cggggattct tttttgtctt tcttgctgcg
     9361 tttcgttgaa aaaaatggct tttttgcttt aaagggagta taatcgaaat gcattaaatg
     9421 atctggaagc cggcaaggtg gatctgctgg gaccgctcct aaagaatgaa tatacagaag
     9481 agatttataa ttttccggaa caggattacg gcacggttta tacaacgctc tctgtgcact
     9541 ggaaaccgga aatctgcgtg aggataacgc tgcttctgtt gtgccgctga aagtaggact
     9601 gtgggaacag gcaaaaacaa gaaatcagga agtgatctca aatgtttctc ttgatccgat
     9661 ttccggaacc aggattatag agagatttgc accaaggccg tattattttg catctgagga
     9721 cgaggatctg ctgaaagaag tggatgaggc gatcagcatc atcaagcagg tgcagccgag
     9781 ccttccggat gtactgtttg ataaatattt ccgcaataca agatatgttt tcgcaccgac
     9841 acagcagcag aaagactatc tgaaatcgat ggatacgctg gaagtgctgt gtgtggagcg
     9901 agatgctcct tatgtttatc agaaagaggg aaaaccggca ggaatgatgg tggaaatcct
     9961 gaatagtttt gcagaagaga ccggagtttc gatcaattat acattctgcc agagccgcga
    10021 tgaggctgaa cagaaattaa aagaagaatc ttatgacctg atgatcggtc tttcttttga
    10081 ttcagaatac tgtgcacgtc ttggatttgt gcgaagcaag tctattatgg agtccaatct
    10141 ggcttatctg catcgttcgg agaacagcag ccacaaaacg gttgcgattg aaaaaggaat
    10201 ggaaagcctg gtagatacaa cggaatttca ggaagtgata aaatgtgata atatcgtaca
    10261 gtgcatggaa gcggtcacaa acggaagagc agattatgca gtcggagacc gttcagccct
    10321 ggaatattat atgtatgata cttacagcac actggttacc ggcctgatct cgggaagtgc
    10381 acagaatatc tgtattgcga tcgcaagaga aagcaatctg cagtttataa ggcttgtgaa
    10441 tgactatatt tacagcctgt cggatctgca gaaaacaaca tttctggaag aaggaaatat
    10501 gcacttgtat aaaacatcgc tgcgaggcta tgtacggatg catccgatcc agacgatgct
    10561 gctgttttcc ggactgactg catttattgt ggttgcatgt tccatgatgt ttcacgcgaa
    10621 acgaatgcat aaaaagaata tggaactgga aatggcgaat caggcgaaaa gcgaatttct
    10681 gaccagaatg tcccacgata tccgcacgcc gatgaatggt atcatcggta tgctggatat
    10741 ttcggataaa tgtctggaca atccggaggc ggtaagaaaa tatcataaaa agatccgcgt
    10801 ggcgtcggag tatctgctgt cgctgatcaa tgatgttctg gatatgagca aactggattc
    10861 gggggaaatg tattttgcgg aagaatccgt gtatctgcgg gaaatgctgg aaaactgtag
    10921 ggatatcatg gagacaagag cggcagaaca tgggatcgaa ctggaaacgc cggggcttga
    10981 aaactttaac ccgccgcggg ttctgacaag tgaactgcat atccgccaga tcgccatgaa
    11041 tatcatcagc aatgcgatca aatacaataa gccaaacgga aagatcatag caacggcgga
    11101 agtcgtaaat cagacagaag attacgttac ctgcagattt atggtaaaag ataccggaat
    11161 cggcatgagt gatgattttc agaaaaatat gtttgatcca ttctcgcagg aatacggaaa
    11221 agaccgcagt gagtttaggg aaaccggact tgaactttct atcgtgaagc ggatcgtaga
    11281 ttagatgggc ggcgaaatcc gggtcgagag caaaaaagga gtcggaacta cctttacctg
    11341 gacgttgaca tttaaaacag ataaagaata ccgggaagaa aaagtggcgg caaaccagca
    11401 ggacttcagt ctggctggga aaaagatcct ggcagcagaa gataatacct taaatgcaga
    11461 aattctgata ttcctgctgg atgatatggg agcggacacg gtgcttgtgg aaaacggaaa
    11521 acaggttgta gaggcattta aagaatccga ctgggatgaa tatgactgca ttcttatgga
    11581 cgttatgatg ccgatcatgg acggttacac ggcaactcgc agaatccgcg gtctgtcaag
    11641 gccggatgca cagagcatac cgatcatcgc cctgacagca aatgcatttg cagaagacag
    11701 aaagaaagca atggaagccg gtatgaatga ccacgtcgca aaaccggtcg atatgaaggt
    11761 actgacagca gtgctgcaaa agtatctgga gaaataaaaa caatatcata gaagagagag
    11821 caaaagaaca tgaaaatgca tctctgtaca gagacaagct gaagaaataa aaaagcaata
    11881 tagaaacaga aagggcataa tatctgagat atgggacatt tttattttgt acgacatgga
    11941 cagaccatct ggaatgtgga aaataaaatc tgcggtgcga ccgacattgc actgacagaa
    12001 ttcggacatc agcaggcgat cgaaaccgga agaaagattc tggcagaagg cattaaggca
    12061 gatgagattt tatattctcc gctgatcaga gcggcggata ctgccagaca tatttcggag
    12121 atcaccggaa taccggcaag agtcgagccg cgtctgacag aacagcgttt tggaaagtgg
    12181 gaagctaccg caagaaacgg cgaagcattt aagaaagcaa aagaatgttt cgcgtgcagc
    12241 cacgaaggag gagaatctac tctgcatctg gcacagcgga tctataatct gctggatgag
    12301 atcaaacagg aatcggacga aaaaacctat attcttgtgg cacacaatgg aattgcgaga
    12361 gtggtgcagt cctatttcta cgatatgaca aacgaagaat acgctgcttt cggagtgaaa
    12421 aactgtgcgg tagtacggta tgatttttaa aatagttttg gaaaagctct gcaaaaagca
    12481 aacgtgggag ttcaggaatc ggttatgaga gatatagtgt atgcaggtgt tgtggggtgt
    12541 gttatggatg gaaatattgc ataaatatac agcaaaattg agttggatgg gaaagggaca
    12601 tgaagaaata tcagttttac ggctgggaac aggcagatgt tccggcaact tcaaaaacat
    12661 atgaaaaaat aaaaaatcca aaagaattat atgatatatt atcagaaatt tggtgtgcgg
    12721 atacctgtgc accgagaatg agggaaagat ggagcaaaga gaatcaaaca ttaggacaat
    12781 gttctattac ggcatttctg gcacaggata tctttggcgg aaaagtgtat gggattctca
    12841 gaccgggcgg aaattatcat tgttataatg tggtggggga ttgttgcttt gatctgacaa
    12901 gcgaacagtt tggcgatgaa attcttgatt acagagagaa tccggagcag ttccgcgagg
    12961 tacatttcca gaaagaagag aaaaggcaga gatacgagta tctgaaaaaa gaactggaaa
    13021 catatctcgg cagggcatcg gagcagacga aacagcttta caaagtatta ttaagcaaag
    13081 gttaccccaa agaactttgt gtggagatag cctacaaaaa tatgaacaca gactatacgg
    13141 cgaccaggat gttaggttat ctgtaccgcg tgacaaatcc aaggattgaa gatttagtag
    13201 atgaaatgct ggcaattctg agtgacaggg aagcaatcat acaaaagaag gaactggagc
    13261 acgcacaggc agtgataaat gatatgtata aaaatggact gtaaaaacat ggttcacgtg
    13321 aaaaaatccg gttgagaaaa gagtttaacg aaagaactta gccggatttt tttgtatata
    13381 acagtaaaag aatattataa gcggttatta cgaaaaagga tggataaaaa atggtcagaa
    13441 caatcgtaaa agatattttc tttctgggac agaaatccga gccggcaaca aagtcggata
    13501 tttctgtagg gaaagatctt caggatacac tggaagtcaa ccgtgccggc tgcgtgggaa
    13561 tggcagcaaa tatgatcgga atcaaaaaga gagtgatcat tgtaaatata gggctggttg
    13621 atgttgtgat gtttaatccg gtaatgctgc gaaaagatac tccatatgaa acagaagaag
    13681 gatgtctttc gcttacaggt gtgagaaaaa caacacgata cgaaaacata gaagtagagt
    13741 actatgatat ggactggaaa aagcagaaga tgaaactcag cggatggccg gcacagatct
    13801 gtcagcatga gatggatcat ctcgaaggga ttttgattta aaaaattgcg gcggaggttt
    13861 tcttaatgtg taaatgaacg aattccccga agggcacgta aaagtgcctt cttgggaatt
    13921 cgttcatttt gcacatttcc aaaaactcct ggcttgccgg ggattgctga agcggaaagc
    13981 tctttcttgc cgaggtataa aagctgagat gaaagggaca gaagcacagt gatggaaatt
    14041 tatcttagtc gaagaagact cccacttcta taaagtggcg agcaacaaat ttgtcttagt
    14101 gagagttcaa tctccgacta agataaacgc tccgcgagga tgcacagcga ttctgttagg
    14161 taaatgtcag caaagctgac cagaatcgcg tgccaagtct cacgtgcgtt cgacttgaat
    14221 atcgtcactg tgctttttat atatttagtt tcacgtgaaa catagcgatg tgaaacgcaa
    14281 aatgtggttg ttttatagaa aagagaagac aaagagaaaa caactgtact ttttattgga
    14341 catgttgtac tgtataatga agtatagaag acgtggaaag gaaggtatgg ggaatggaga
    14401 atgagaaaaa aatcaaaaaa gtctatttta cgttgacggg tacgaagtat cattatggaa
    14461 tggattttct ggaaaagggt atgaaattat atctggaaaa ggaaccggat aataaatatg
    14521 ataaagaagc aattatggta aaaaaagaag gacttggatt gattggttat gtggcaaaca
    14581 gtacatatac agtaatcgga gaaagtatga gtgcaggtcg tttatatgat aagattggaa
    14641 agaaagcaac agcgaaagtt gttatgctga caaatgcagg tgttatctgc aaaatctgta
    14701 aaaaaagtct gttggagtat cagcagcaaa ataaagaaat cgagaaaagc gaagagccgg
    14761 ttaaacagaa ttaaaagagt ttcacaacgt tttcactcta tatgttatac tgatttcaac
    14821 aatgaaaaag ggagaaaggt atatgcctaa aggtgcaaat caaaagttta aattgtacag
    14881 acttgcacag attatgcagg agaatacaga tgacagccat tatatgacaa tgccggaaat
    14941 tatggttgag ctgaaaaaat atggaataac agcagaaaga aaaagtattt acagtgatct
    15001 gaaagatctg gaacagatgg gcatagaagt tcagggggaa aagacaggaa aatattatca
    15061 gtaccatgtt gtaaatcgcc cttttgaact tgcagaactg aaacttctgg tagatgcaat
    15121 tcagtcatcg aaatttatta cagagagaaa aagcaatgca ctgattaaga aaatagagaa
    15181 aatggtcagc aaatatgagg caatgacact gcaaagacag gtttatgttt ccgggagaat
    15241 taaaacgatg aatgaaagta tctactatac ggtagatgct attcacaatg caatatctga
    15301 aaatcgaaaa attaagtttc aatattttca atggacggaa aagaaagaaa tgaaattgcg
    15361 acatggcgga aagtattatc atatcagtcc gtgggggctt tcgtgggaag gacaaaacta
    15421 ttatctcgta ggttatgatt ctgaagcaga aaaaataaag cattatcggg tggataagat
    15481 gctacatatc caggtgtcag aagaaaagag ggaaggtgaa cagcattttc agcagcttga
    15541 catggctgat tatgcaaaaa agagttttgg aatgtatggg ggaaaagaac agctagtaaa
    15601 attaaaaatt agagatacgt tggtgggagt gatgattgat cgttttggaa aagatgtgat
    15661 gattattcct gaggataaag gtcattttat cgttccggtc tgcgtatatg tcagcagaca
    15721 gtttttaggc tgggtgtttt ctctcgggga cagcatagaa attctttcgc cgccacaagt
    15781 ggttgagcag atgaaagagg agagtgaaag gttgtttcga caataccatt agaaatacag
    15841 aaagagcggc tgaatcatgt aaaatcatga tagcaaaaga taaaaagcaa aaaactattt
    15901 cgatggaggg cattcatatg aactatgtag tgattgatct ggaaatgtgc aaagtaccaa
    15961 aaatgtatcg aaacaaaatg tataaatatg caacagagat cattgaaatt gggacagttt
    16021 tattgaatga agatttcagg cagattgcga cattaagaca gtatgtgcat ccggaatatg
    16081 gagtattgga tcactatatc agcaatttga caggtattca gaatgttcag ataaaaaatg
    16141 caccattact tgaggaagcc ctgaaacatc tgacgaattg gcttggagac agggagtata
    16201 aaatttttgc gtggagtgag tgtgactttg ctcaactgag aagggaaata aaaagtaaac
    16261 agatagaaga tgagtaaatc caaaatttca tgcagccaaa gcgttggatt gattatcagg
    16321 atgcatttgt aaaacgaagc ggattttcca gggcaatctc tttaagtgaa gctctgatgc
    16381 tttgcgatat gcagccttgc ggcaggttac acgatggttt ggatgatgca atcaataccg
    16441 caaaattaat tgagatgctg gaatctgatc cagattacca gattcgtaac tacgagaaag
    16501 aacttgcaat ttcagcggag ccactgcagt tttgcatggg agatttgttt gcggggttaa
    16561 gcttgagtgc atagtatgcc aggtcgcata tgaattcgac ttgaattgat aaaaatgcca
    16621 gaataatcat tttcaaatta ttctggcatt tttatccttt ggaattcttt cggatatttt
    16681 gtgtgagatt gaaagcggta ttcccgtgta ggtggtttca cgtgaaacat taaaatattt
    16741 gatttctaaa gtactctgtg tttcacgtga aacgaaaaaa ttatttttca aagaaaagtt
    16801 tcatgtcatt ttctacagta tctattccgg cgattccgaa gttttccata agtacttttg
    16861 ctacatttgg actgagaaat gcagggagtg tcggtccgag gtgaatattt ttcactccaa
    16921 gatacaggag ggagagcagt acaataactg ctttctgttc gtaccatgcg atattgtaga
    16981 tgattggcag ttcattgata tcgtcaagcc caaatacttc ttttaatttc aatgcgatca
    17041 cagccaggga gtaagaatca ttgcactgac cggcatctaa aacgcgaggg attccgttga
    17101 tatctccaag attcagttta ttatatttat attttgcaca gccggcagtg aggatgacag
    17161 tgtcttttgg aagagctttc gcaaagtcgc tgtaatagtt acggctcttt gcacgtccgt
    17221 cacagcctgc cattactacg aattttttga tggaaccgga ttttacggca gctacaatct
    17281 gatcagcaag agccagtacc tgtgcatgtg caaatccgcc gataatttcc ccttcttcaa
    17341 tctgagcagg tggggcacat ttttttgcgt gttcgatgat cacagtgaaa tctttgttca
    17401 cagcagaagc actgccgttt tctgctgaaa tatctccaat atgctgacag ccaggatatc
    17461 cggctgcacc ggttgtgtat aaacggtctt tatagctgtc ttttggcgga acgatacagt
    17521 tggttgtcat caggatcgga ccgtggaaag cttcaaattc atctttctgt ttccaccatg
    17581 cattcccata atttcccaca aagttttgat attttttaaa agccgggtag tagtgtgccg
    17641 gaagcatttc ggagtgtgta tagacatcta cgccagttcc ctgagtctgt tccagcagca
    17701 tttccaggtc tttcaaatca tgaccggata cgagaatggc agggttgttt ctgacgccga
    17761 tattgacttt ggtaatttcc ggattaccat aagtttctgt gttggctttg tcgaggagag
    17821 ccatgccatc cacaccatat ttgccgactt ccagagccag ggcaaccagt tcctctgcac
    17881 ctaaagaatc atcaagcgtt gcagccagag ccttctgaag aaaagcatcc acgttttcat
    17941 catcgtgcag aagggcattg gcgtgtttgg agtaagcaga taatcctttt aatccataag
    18001 taatcaactc acgcagggaa cggatatctt cgtcttttgt agacaataca cctacttctg
    18061 cagctttttc ttcaaaactg tcttcagtgc catcccagag ggctgcggat ggaagagaag
    18121 aagatgtgga gagctgtttg ataaggtttc ttttcacttc gagcgaagtg tggatacgtg
    18181 aaacaatcgc atctttatca aaatttgcat ttgttattgt gacaaaaagg tttaaggtga
    18241 tcaggtgatt gatgtcggta ctgatctcaa cgccttctgc ccgaagctgt gtggttactg
    18301 cggacagacc ttttgttaca aatactagaa gatcctgcat ggcggccaga tctggtttct
    18361 ttccacagac tccgactctg gtacatccgg tgcatcctgc ggtttcctga cactgatagc
    18421 aaaacatagt ttgattcata aatgaaatcc tcctttaaga tgacagtgca aactgtcttt
    18481 tatttatctc agtcgagaag actgactcct acttctgcaa gtggtgagta ataacaagtc
    18541 tttctcagtg ggagtacaat ctccgattga gatatgctct gtacgggcga tgcctgcttc
    18601 gtattcttat ctcaatatca tttgaaaaaa gttattctct tttgtataat gcttgtattg
    18661 tgtttgtcat gtgagtaaaa gtatctactg aggcaggtct tcctggtact gcacgtgacg
    18721 tttgtaaata taatggaaaa atgaaaagaa atcggttgtt ttaacaacga aggagaaaaa
    18781 tatatgttga aaaaaatgga aataaaagat tttgaccagg tgtatacctt gatggaagaa
    18841 agttttccga aagacgaata cagaaattat cagaaacaga aggaactgtt tcaaaatcca
    18901 gagtatcaga ttttgataga aaaagatcca gagctccggg aaatcaaagc atttcttgca
    18961 gcatgggagt ttgaaacgtt tatttacgtg gaacatttcg cagtcaatcc ggtattgaga
    19021 aatggaggcg taggaagcag gatgctaaaa gcgttgacag aagacgcaga aaaaatgatc
    19081 attctggaag tagaacctcc gacagaagaa atggcagtaa ggagagtcag gttttacgag
    19141 agaaatggat tcttctttaa tgattatcca tacattcagc catccatggg tgaagggcgt
    19201 aaagagacac cgctttttct gatgagcaca gagagaaaaa ttgacgaaga agagtacaga
    19261 atgattcgca atacactgta tacaaaagtt tatggaaaga aagacatgct ggaaagagaa
    19321 gtgtagaatg tgcagtgatt ctgagaagaa tatgtcagct ttcgctgacc agaatcacgc
    19381 accaagtctc acgtgcgttc gacttgaatg atatgtgtta gtttcacgtg aaacataatt
    19441 tcggataagc taatttggac ggatacgcgt gaggtttaga acacgattcg gatcaggtca
    19501 gccagctgac tgaaacaaat gctccactga taacaaattt gtgtcagggt ggtgaaatgc
    19561 aatgctctgt ctgatataga tttgacggat tatggaagtg cacagaagaa aaataaaaag
    19621 agcatataag ctaaaaaaga aaatgctttg gcttatatgc tctctttgta taccggatgt
    19681 gagatgactt ctgcctctac gatcaatcag aggtattctt gggtgttaat gatcagtggt
    19741 gaccacagcc gtgatttcca caggcgtgtc cttcttcgtg actgtggtgg ctgcatgtag
    19801 caccggcaga ggtcagctcg ccacgcaggt aagcttcgac tgctttgtct gtgtctccgg
    19861 atgcaccgga acataaggta atgccggcat ttgcgagagc tacctgagca ccgccgccga
    19921 tgcctccgca gatcagtaca tcgatctggt taccggaaag aagtccagca agagcaccgt
    19981 gtccaatacc atttgagccc attacctggc tgtttacgat tttattgttt tccacttcat
    20041 aaattttaaa attttctgtt cttccaaaat gctggaatac ttctccgttt tcatatgtta
    20101 cagcaattct catgacgttt tctccctttt tctgcatata atttactggc tgcgtggatt
    20161 tacagcagtt ttcacagcat ggacttccat cacataaaat gtaatttcca ccctcaatgc
    20221 ggatggattt tccaaggaca atgctttcgg caattttttt tcgtgcggaa ttataaatca
    20281 actgtgcagt tgtacgtgca acacacatct tttttgcaca ttcttcctgg cttagacctt
    20341 ccatatcaat ttgccgtatg gcttcgtatt cgtctactgt cagtgtagtt acatcgtcca
    20401 gtccgttttc agactgaaaa atcacataat caggttctct gcaaatcctt ctttttctac
    20461 attttggcat aagtttttct ccttttattg gcatatgcca ataaaaataa tagcataggt
    20521 aaattaaaaa gtaaagagga catttgaaat cagaggtcag gcattcaaag aatcgaacat
    20581 ttctgacctg aaacagggaa tttcgaatta cagttttggc tggtatcgca tgctggatac
    20641 gttgtaaacg gtaacgaatg cctgcggatc ttcttttgta atatagttca taagtttctg
    20701 atattcagat ttgtccacga tggtaatgat ctcgtttctc ttttgcatnn nnnnnnnncc
    20761 gatggattca taaatggtag ctccgctgtg cagatcgtga atgataaaat tacgaagttt
    20821 ttcttctttt tttgtaataa tgcagacacg acgcttgatg ttatggtcga aaataaagtg
    20881 gtcaagaacc ataccgttga aataagttcc gaggatactg agcactacgg tcttcttatc
    20941 gtagacgagt gcggcagaaa gtgcaacaca cattccggaa agagacatgg cttttcctaa
    21001 atctatatgt gcatatttat tcaatatttt tgcaacaata tccaatccgc cggaagaagc
    21061 attgcggtta aaaaggatgc ttaatccgac actgacaacg agaatatagc agagcacatc
    21121 cagttcctga ctgtttgtca tggagccaaa atctggcagg atcatttcaa agagccagag
    21181 gaagaatgga agcataatgc tggtataaac ggttttaacg ccaaattccc tgccacaggt
    21241 gaaaaaaccg atgatcagga gagtaatgtt caggatcatg ctgatcgcag acagagggag
    21301 cggaataaag tttgtcagta caataccaag tccggaaata ctgctgacgg aggtatgact
    21361 tggaacaaga aaaaagtaaa ctgcagcggc aatgatcgca acagcaaccg tcagaataaa
    21421 ggtttctttc aggatgttta aatagtttgt ttttgttttc attatttgcc tcatttatca
    21481 ataaaattta gtatccgcaa caagaatgcc agggcatcct tatatattta aattaaaaaa
    21541 atcagataaa aaccaatagt tattgctgaa aatcatacaa tttcgatgga ttcaagatca
    21601 ttcggaatgt aaagattgcc ggaaaaataa gattctcctt cggctgaata cagttcttta
    21661 cggttctgga tgtttttgtc ctctgtgtga tagagaacaa ggttcggaac atgaagcttc
    21721 tgggcaagtt cacaggcgtc ttttacggtt gaatggtgct tctcgtaagg gttgaaaagg
    21781 tctctttcgg agtagaggca gaaagcttcg tgcaggagcc attcgcagcc ttttgcgtaa
    21841 ggcttttcac agtcgttata aggctcatcg ccgcaataga cgagcttttt gccactgctc
    21901 agttccatag aaaagccaaa ttgctttgct ttggtggatc cgatatcgaa aaaggtcaca
    21961 tttcggtcgt tgatagtgcg agtttcagcg tcggaaacac agatcagatg cagcctgtcg
    22021 ccgataaaac gagtctgttt ctttgggagg agctgttcgg ccaggttatg gagcagggaa
    22081 ataacctctg cgtgaccata aatagtgact tctccttcat atttgccact gttcatattc
    22141 tggcagatca cgcgaatcat ccagatgata ccggtcagat ggtcgatgtg tttgtgggtg
    22201 ataaagattt cacgcatatc catccagtta aaaccggcgt gttttaactg atgaagaatg
    22261 gtgctgccgc caccgccgtc caccataaaa tatttttcat tttcttccag gacaaaacag
    22321 gtattgtaac attcggttac aagtgcgttt ccggttccta acattgttaa tttcatatgt
    22381 aacaccttcc tttgaaaaaa cagtaaagag gaaatctgct acggtaaaaa gaaacagatt
    22441 tctggttaca agtttccgtt aaaatgtccg ttttagaggc ttaaatatga atttccaata
    22501 tattgtacat aagaaaatga aataatgtgg gaacgatttc atattgcaaa aaatgccgga
    22561 aaatcaaggt ttttcgggaa tatgagtatt gagaaaagct tcatatctga cccaaaacag
    22621 acatataatt attacaggaa tgctgtttac agcctgtaaa ccgtcggata tacgctgcat
    22681 ataaggagat gcaggctatc caaagtatat tttgcagtaa aaaaggaaat attgcaagag
    22741 aaataaatgg gtgctttcta aataaaacat cacatataat ggtgtttatg cataaaaata
    22801 aacgctttat gcataaatgt
//
LOCUS       DAWDWH010000011        21708 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_1446_length_21708_cov_15.927447, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000011 DAWDWH010000000
VERSION     DAWDWH010000011.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 21708)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 21708)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..21708
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1446_length_21708_cov_15.927447"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2911..2920
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6652 a         4104 c         5716 g         5226 t
ORIGIN      
        1 tattgacata ataagaatta ggtggtatag taaccaaaaa atgaaaaaaa ctttcaggaa
       61 gaagcataga gatagatttt caaaaaacag aaaaggaaaa caggtatgga caataaagaa
      121 aagaaaaacg aacaagatat gatcaatacg caggttgaac cgcataatca cagagcgatc
      181 attggattcg acaaaaacgg tgttccgaaa gttctggtgt cggtcagcgg acatacacac
      241 aaacatgtag atgaaaacgg aaacgagtat gaacacagcc acgaggaagt tttcacggaa
      301 gattacatga aggcggttgc agcttatcgc aaaacatttc cgacaaagca ggatgtgctg
      361 gacaatgtgc cggacccggc agtcaaagaa atgatgctgc gtatggagca gcttggatat
      421 gatacggcat ttgaccgttt tgacaaacaa cagccacagt gcagttttgg actgagcggt
      481 acttgctgcc gtatctgtaa catgggtcca tgccgtatca ctccgaaatc tccgagaggt
      541 gtctgtggtg cagatgcaga tctgatcgtt gcgagaaatc tgcttcgcgg tgcggcagcc
      601 ggtgcagcac agcatggcat gcatgcaaga gaactcatcc tgatgctgaa atgggcagct
      661 cagggcaaac tggatattcc gatcatcgga gaatataaag tcattgcaat ggcaaaggca
      721 tttggcatca aaacaaaaca cagacagatc aaaaatatcg cgatcgacct ggcagatgca
      781 cttcttgcag atttatcgag aacagagccg gatgaataca aagtgatcac tgcctgtgcg
      841 gcaccggagc gtcagaaagt atggaaagat atggatattc tgccgatcag tgcttatcac
      901 gaagtgtttg aagcttacca caaaacaggc tgtgcgacgg acggagactg ggagagcgtg
      961 atgaaacagt ttcttcgctg tggacttgcc tttacattca gcggtgttgt ttctacatcg
     1021 atcgcaaccg atgcactgct tggagtcggt gatcgtgtga cttccaaggt aaacgtcgga
     1081 gcattgaaaa aaggctatgt aaatatcgca gttcacggac atctgccgat tctggtaaaa
     1141 gagatcgtaa aggctggaca gagcgagaaa tttcagaaac ttgcgaaaga aaaaggagca
     1201 aagggcattc agttttacgg catctgctgt tccggacttt cttctatgta ccgttatgag
     1261 ggcgttatcc cactgtccaa tgcagtcagt gcagaactgg tgcttggaac cggagcactg
     1321 gatctttggg tagcagatgt gcaggaagtt tatccggcga tcatggatgt tgcaaaatgt
     1381 ttcaagacaa cagttgtcac gacacacgat tctgcaagac ttccgggagc agaacatatc
     1441 ggatacgacc atcatcattc gaatatcgcg gagacaaaaa agattgcaga acgcatcgta
     1501 gagcgtgcaa tcgaaagttt tgaggcacga aaaggtgtgc cggtatttat tccgaaatat
     1561 gaagtagagg cagaagttgg attttccgta gaatatctgt gtaaaaaata cggcagtctg
     1621 gagccgattg cagatgcaat ccgagagggc aaaatccttg gtgtggtaaa tatggtcgga
     1681 tgcaacaatc cgaaagtact ctacgaaaaa gcgatccttg atgtctgtga tgtacttctg
     1741 aaaaataacg ttctgatcat cacaaacgga tgtgcatcct tcccgctgat gaaaatgggt
     1801 tattgtcaga cttccgagtt tgcttacagt aaggcaggag aaggcttaag agaatttctc
     1861 aaaccggata tgcctccggt atggcatgtc ggagaatgta tcgacaacac cagatcatcc
     1921 ggaatctttg caggcattgc aaatgcattc ggaaaagcaa tgtacgaaat gccgttcgcc
     1981 tttgcatcac cggagtggtc aaatgaaaaa ggaattgatg cggcacttgg cttccgcctg
     2041 aacggaatca gttcttacca ctgtgtggaa gcaccgatcc atggttcttc aaaagtcatc
     2101 gaatttttga aagaaggaac aaaagaaacg ctgcattctt ccatggtggt agatgtggat
     2161 ccggtatcac tcggcgagaa aatggtagct gatatgaaag aaaaacgcag acagcttgga
     2221 atctgatcaa aaattgcgaa acgataaagt acagaaaaat ccagatgaca ttttttaaaa
     2281 atgccatgag cgtttgattt aaatagctgc ccgaaaacag acataggagt acttatgaga
     2341 aaatataaaa tcagaaaaag cctgtgcatg ctgcttgtcc tgacgcttct gacagttttg
     2401 tcctcaggat gcggcagatc cgaggcaaaa tataccgcag acggaaagaa gcagatcatc
     2461 acgatcggct accttccgat cacacatgca ctggccgtct ttgaagaaaa agaattgctt
     2521 gatgcagacg aaaacagcac tctgcagata cagcttcaga aattcagttc ctggaccgat
     2581 ctgatggatg cattaaattc cggaaggatc aacggagcgt ccgtgctggc agaacttgcg
     2641 atggcagccg tcagtaacgg actggatctc aaagcagtgg cactgggaca taaagatgga
     2701 aatgtggtga tcgtttccaa tgacatcaca aaagtagaag atctgaaagg caaaaccttt
     2761 gccattccat ctaaacagtc ttcccacaat attttactga acgatatgct ttctgatgcg
     2821 ggaatgtcga ttgacgatgt aaaggttgtc cagcttgcac cggcagaaat gccgtcttcc
     2881 ctggcaagcg gtgcaattga tggatattgc nnnnnnnnnn gttcggagca caggctgtgg
     2941 tgcaggatta cggccatgtg ctttatgatt ccacacagct gtggcagaat tccatctgct
     3001 gtgccctcgt tatgaatgga agctttcttg aaaaaaacaa ggaagccgtt gataaactgg
     3061 ttgccggata cgaagaggcc ggaaacaggc tcgacgaaac aacggcaaaa acacttgcag
     3121 aagcttatct tggacagaat gaaaagacgc tggaagtttc catgcagtgg attcattatg
     3181 acaatctgca gatcacaaaa tccgattacg aacagctgac agaacgaatg aagaagtacg
     3241 gaatcaacaa aaatccaccg tcttatgaag actttgtata tcagcctgaa agcagcgaat
     3301 ctgattaaaa tagcgtatgg aaaagaacgg tgcgtcttga aagaaaaagc gaggaaacaa
     3361 tattatgtgg aaaaaaggta tcagagttat cgtgtcattt gtagttgtac ttctggtatg
     3421 gcagatcgcc tgcacagtga caaacgcaaa tgcaacactg tttccgtctc cggtcaaagt
     3481 cggagaggaa tttgttaaat tgtttacaca gggactggaa ggaagtactt cccacgttac
     3541 cttaccggga catatccgga tcagcctgct acgttttgcg accggctatg tgcttgcagc
     3601 cgtatctgcg atcctgctgg gactgatcct cggaagcctt ccgaaagtat ttcagtttat
     3661 caacccgatc cttcagatca tccgtccgat cgcaccggta gcattcctgc cgtttatcgt
     3721 attctggttc ggaatcggtg atgtgcctgc gatcgtgatc atctttatag ccggattctt
     3781 cccgattctg ctctccgttg tggcggcggt acagcatgta gatgggattt attataaaat
     3841 cgccaacagc ttcggagttg gaaaagtaaa gacattattt ggcattgtat ttccggcgat
     3901 ttttacacag attgcaaatg cacttcgcct ggcgcttgga acatcctgga tttttctggt
     3961 ttccggagag atggtaggtg cacagtccgg acttggattt ctgattatgg acagcaaaaa
     4021 ctgcatgcgt tttgatgcac ttctggcaac catgatcacg atcggagtga tcggatttat
     4081 cttagatggc ttgttacgtt tcgcagaaaa agcgatcagc acaaaaattg gatttggagt
     4141 gtagagaaat gtatatagac gtatcaaatg taagcatttc cttcgaggaa aacaaagaac
     4201 agcataccat tttaaatgat gtttcccttc aggtggaaaa aggagaattt atctgcctgc
     4261 tcggaccgtc cggctgcgga aaatccacac tgctcaatgc gatggccgga tttttaaaac
     4321 cttcatccgg agagatcaaa atcgacggac agaccgtaga aaaaccgtcc atgaaatatg
     4381 tcaccatttt tcagaactat ggactcctgc catggagaag cgtgcagaaa aatgtagagc
     4441 ttggactgga aacaaagaaa ctcggcagag aagaaaaaaa gaagatcaca gatcattacc
     4501 tggaaatggt tggcttaaaa gaagcagcta aaaaacgtcc ggcacagctt tccggcggta
     4561 tgcagcagag ggtggcaatc gccagagcac tggcggtcga gccggaaatc ctgtttatgg
     4621 atgagccgtt cggggcactg gatgcgatca ccagaatgaa acttcagaaa gatgttctga
     4681 agatcgcaca tgagacagac aaaaccgtcg tgttcgttac acatgatatc gaagaagcta
     4741 ttttccttgc agaccgcatc gtgatcatgt cagccaatcc gggcaagatc aagagcatca
     4801 tgaaagtcac actgccgaaa gacagagacc gtacatcaga tgatttcctg ctgatgcgag
     4861 ataaagtgta tgagatcttc cagatgaaag aagagagaaa tatcgagtac tttatctgat
     4921 ttcacaagat tcatttctat tgtttgtgaa taataaaaag agaacatata aaaatatatt
     4981 tgacataaaa gaatagaaat gctattataa atgcataaaa ggtgttgccg atgacggttc
     5041 gcctgattgt ttaatagttg gataaccgcc tatgttttgt caggacagag ggcggttatt
     5101 tttgcgtctt ggagtcatgc tttccatgca tataaccaag actgtaaaaa gtcgcacaca
     5161 gtgaaaatac tgcgataagg tcagaaatgg taagcataag taagacctcc ttttccaaga
     5221 tttctctgaa agtgagagat aaatctatgt aatcggagct acagtctact ctccgcaaga
     5281 agcgaaccgc catacacacg tcaacgtgtg gcaacacctg ttttgtataa tatcatatat
     5341 ttattataaa atcaataaaa acaaaaaaag acattaataa acaaaaagaa acatacaaaa
     5401 acaaaaataa tatatttgac ataaaagaat agaaatgcta ttataaatgc ataaaaggtg
     5461 ttgccgatga cggttcgcct gattgttaaa ggcttatgat cgccctagtt tctcaggcat
     5521 ggggcggtca tttttctata tagaatcgca acaatggagt gaataacgat ggtaaaatat
     5581 ttgataaagg acacgacaag ggaagaacgt gagcggatcg tagcagaatc tcttggcaat
     5641 atcgatgcct cctgtgacgg atgcagttca gggatcgtag agatgtatca ggattatatt
     5701 gatgggaaaa aggaactgcg tgagatttcg atggaattta acgcacgtta tatttccggg
     5761 gaggaagcac caacggttcg ttcttcctgt ccgatgagat aacaaaggag cggatgatgg
     5821 caaaagcggg cggaaattta ttaaacactt atgttgcgga ttatgtggtc tttgatctgg
     5881 agacgaccgg gatttcgcct aagacggacg aagtggttga gatttctgca gtgaaggtgg
     5941 agcacggcaa ggttacggat gagttcagta cgctggtgaa tccgaaacga aggattcctt
     6001 acggagccag ccgtgtaaat ggcattacgg atgatatggt tgcggaagca ccgttttttg
     6061 aacaggttct ggaagaattt ctggagttta ttgaaggatt cgtgttggtc ggacataaca
     6121 ttgcccgatt tgacatgaat tttctttatc gtgatgtgga aaagtatttt gaacggtcgc
     6181 tgccaaatga ttatattgat acattgcaga tggcgagaag ggagcttccg aatctggagc
     6241 atcacaggct gacggatctg gcagaatact acgggatttc ggcagaaggg gcacatcggg
     6301 cattaaatga ctgccggatg aaccagcagg tttttgagaa aatgggaaat cctgtataca
     6361 aaaaacaggg caaagataaa gaaaataaca gaggagtcga ttcggcaaaa acagaaccga
     6421 gtgaacaggc acaaaacacc gcaccgacag tccggaaacg cagtactttt aaactgcgtg
     6481 cggtggtgga gcggatcact taccagaatc cggacaacgg ctatacggtt ctgaagtgtg
     6541 cggtgaaaaa ttatcaggat ctggtgacgg tagtcggcag tcttctggat gtcaatgttg
     6601 gttcggtgct tctgatcgac ggaaactgga aaatggacag tcgctatggc agacagtttc
     6661 aggcaaagac ctgggaagag acgatgccgg cgactgtttt tggcattgaa aaatatttgg
     6721 gttctgggct gatcaaaggg gttggtccca aatatgcaaa acggatcgtg cagaagttcg
     6781 gaacagagac gatcgctgtg attgagaccg atgtcttccg gctgagggaa gtggagggaa
     6841 tcggagaaaa gcgtatccga aagattgcag agagctggga aaagcaaaaa gagattaaac
     6901 atgttatgct gtttttgcag gatcatgggg ttagcacttc ctttgcagca aagatctaca
     6961 agcaatacgg caatgaaagt gtcaataagg tccgggaaaa tccatttcgg ctggcagatg
     7021 atatctgggg gatcggcttt aaaacagcgg atgggattgc acagaagctg ggatttggaa
     7081 aagaagcttt tgtgcgtctt cgaagcggta ttatgtatac attaagcaat ctggcggatc
     7141 agggacatgt ctatgcaacg aaggaacagc tgatcaaaac ggcagcagag cttctggaag
     7201 cagaggaggg ctgtgttgtg atgacgctgg atcagatgct ggcggatcac gatctgatct
     7261 tacagaaagt ggaaattccg aaagagcagc gaaaacatga aatgcctgca gaccttgaac
     7321 aaccggaatg gaaattggca gaacctcaaa taaccgaagc tatttatctg ccgccgtttt
     7381 attatgcgga gaccggtgtg gcaggaaaat taaaaaggct gctggcagct ccggcggcgg
     7441 atcgtctgtg gactgcactg gagacggcga aagaaaaaac aggcaatccg gatctgtcga
     7501 ttgatgtgga gaagattcag gaaaaggtgc atatggagta tgatgaggtt caggcagatg
     7561 cgattcgcaa agcagcggtt tccaaagtta tggtgctgac cggaggacct ggaaccggaa
     7621 agacaacgac cacacagggg atcattgcag catatcgggc attcggactt aagattctgc
     7681 ttgcggcacc gacagggcgt gcggcaaaac gtatgacaga ggccaccggc ctggaagcga
     7741 aaacaatcca tcgtcttctg gaatgtcagc ctccggaagg atatctcaaa aatgaagaaa
     7801 atccgctgga aggggacgta ctgatcgttg acgaatgttc gatgatcgat atcgttttaa
     7861 tgaatgcact tttaaaggcg attccgcagg ggatgcgtct gattttggtc ggagatatcg
     7921 atcagctgcc gtctgtcgga gccggaaacg tgctgcgtga tatcattgat tccgaaacag
     7981 tgcctgtgat ccgtctgacc aggattttcc gacaggcaca gagcagccgt atcattatga
     8041 atgcacatcg gatcaacgag ggcaaaatgc cggatatttc caatgggaag gatacggact
     8101 tcttttttgt gcagaaagaa gatccgcagg aggcagcaga cgaggtcatg cgtctggtga
     8161 aaaataagct gtcgcgttat tatcagacgc cgccgtctca gattcaggtg ctcacgccga
     8221 tgcagaaagg aatcgtcgga gctgccaatt taaatacgat tcttcaggaa actctcaatc
     8281 cgcagggcga aggactgcga agaggcggtt ttgtatttcg cgtaaacgat aaagtgatgc
     8341 agatccgcaa taattatgac aaagaagtgt ttaacggcga tatcgggatg atcgatgtgg
     8401 tggatgtggc agcccgaaat ctgaaagtga atttcgacgg acgcatcgtg gagtatgatg
     8461 tcacagaact ggatgaactg acacttgcct atgcaacaac aatccataaa gcacagggat
     8521 cggaatatcc gatcgtcgtg atgccggtgc tgatgaatca ttttgtgatg cttcagagaa
     8581 atctgatcta tacaggaatc acccgtgcga aaaaaatcct cgtactggtc ggaacacgca
     8641 aggcactttc ctatgccgtg cggaatgtga cggtgacaaa acgaaatact cttttaaaag
     8701 aaaggttaca ggaaaagttt taatagctgg acgtgagata aaaaacatag tataataaga
     8761 aaacaatggt gtcggagacg gtattttata tggacattga agaagcggga acagttcggg
     8821 ggggtgaact gtttttttag ttttggaaga cgcagaaaat gtttagtaca gggtattctt
     8881 ttaagaggga agatgaagaa tgcagaaaat tttgatagta gatgattcgg aaataaacag
     8941 agagatcctg gcggccatgc ttgagaaaac atacgagatc gacatggcga aagacggtca
     9001 ggaagcgatc gagatcctgg aaaagaagtg gaaaacgtat cagatcgtgc tgcttgatct
     9061 gaatatgccg gtgatgaatg gatatgaagt tctgaaggtc atggaagaaa agcagtggct
     9121 tgattcactg ccggttatct gtatctcggc agaaacatcg gaggcaagta taggaaaggc
     9181 gtacgaactc ggagccacgg attattttac gaggccgttt gatacggcag tcgtactgcg
     9241 gcgtgtgcag aatacgattg cactgtatgc aaaagcgtcc agcagtctgc acgatgcgat
     9301 ggagatgtta tccggcattt tttatcgtat cattaaaatc aatctgagta tggataccta
     9361 tagaacactt aaaaatattt ccgacgattt caggagtccg ctggatggca ttaagagtat
     9421 ttcgcagaga ctgatgcagt acggagaaga atatgtccat gaggaagatc aggaagcttt
     9481 tttggaattt tgtgattcgg aaaatctgcg taaaaatttc tcggaagaag atacagagcg
     9541 tatcagtatg agctatcgct gtcggaatgg acaggaattt cgctgggttt ccatggagat
     9601 catcaagagt acagagtacg caaaagataa cgaagtcgtg gttctttatc ttcgggatat
     9661 caatacagac tatctgaaac agctggatat ggttctgagc aggagcaaag gttttgtcgg
     9721 tatggtgaat gtcaatgtaa cagacggaac ctgcaaatcc ggaagcagtt ctctgaaaaa
     9781 tctggagctt caggaagagg atcgtacact tgatcagtat ataaacagga tcagcacttc
     9841 gattccacag gaggatgggc gcagaagttt tacagaaaat ttcagcagaa agcatatgct
     9901 ggattctttc gaaaaaggtc acacggtcat tcaggaaaat tatccggtat acagcacaga
     9961 aaaccatcgt ccgcagcttt attgcgtaac agcagagatg gttcgcaatg cttttttcgg
    10021 tcagatagag gctattcttt attttaccaa tattacggag aagtatctgg aagaaaagat
    10081 gccgcagatc ctgtatcaga aaagctatga gcggattgcg gtgattgact taaaacgcag
    10141 gaagatcgcg atcagttctg cgaaagtttt tgatttttca gagtctctgg atcaggagct
    10201 ggattatgaa cagtatctga aaaaagcagt acagaattct gttccggaag aagaaaaggc
    10261 acagtttgaa aatcatacga gacttgataa tataaaaaga gaactggatc tgaaaggacg
    10321 ttattctttc agtacacacc agataaacaa agatggagaa aaatgcctga ttaattatac
    10381 ttatctttat tttgatcggt attttggcat cgtggctgtg gcagtcgagg atattacgga
    10441 gctggcagga caggatgcac tgacaggcgg atacaaccga cagggatttg tacagaaggc
    10501 agaacatatc ctgcagaatg caaatgagga tgacaattat gcgatcctgt ttttcaatat
    10561 taagaacttt aaggcagtga acgaactttt tggaattgat cttggtgatt ttattctgcg
    10621 tgagttttat gaagatctga aaaattcaca gcttgatccg gttgtgaccg ccagggctga
    10681 ggctgaccat tttgtgtgcc ttgtgaaacg ggaaaatctg gatgaggagt ttctgccaaa
    10741 actctgtacg aagaagattt ccagctccgg aaagaccatc cgtctgttca gccgttgcgg
    10801 tattttctat gtaaaagata aagaaatgtc cgtaaacatg atgatcgggc gtgcgaagct
    10861 ggcaaaggaa tatattgaag atgagtatgt tcagccatat aaagtatatg attcttccat
    10921 gcagactgct tacatagaca aagcagagat tgcaggagaa cttgcagaag gcatcgcgaa
    10981 aggagaactt caggtttatt atcagcctgt tgtggatgta aagaccggac agatcgcttc
    11041 cgcagaagcc ctgatccgct ggcatcattc cgagaaaggt ttcctgtcgc caggcttctt
    11101 tataccggct ctggaagaga gcggtcacat ctccgaactg gatgattatg tcattcatca
    11161 ggtcagcgat ttcctgatgg accgttacca tgcaggcaag aatgtggttc cggtttcggt
    11221 caatttatca tggatggatt tctatgatga gaagatcatg gaagatatcc ttgataaatg
    11281 taaaaacaat gatctggaag aaaaactgat ccgtctggaa gttactgaga catcctacgc
    11341 tgcgatgggt gagagccgca acagtattct tgaatccttc cgtacagaag gtgtcaagat
    11401 catgatggat gacttcggaa ccggttattc ttcgtttggt atgcttcagc agtacaattt
    11461 cgatattatg aagattgaca tgagtttcat ccgccagatc gagacaaatc caaagacgaa
    11521 aagtatgtta cgattcctga tcgatatggc acatgagatg ggcattcata tcgttgcaga
    11581 aggcgttgag acagaagttc aggcagattt tctgaagaag agccactgtg attatatcca
    11641 gggatattat tattaccgtc cgatcccaca ggaagagttt cggaaacttc tcgatgagat
    11701 gtaaagattt tttaacttgc aattcaaaaa ttgccatgct atacttgaaa tgatattagg
    11761 gtcagaagac cttttgactc tggaatctaa tacatgaagc agaaatgatt ttcatgcaaa
    11821 tatttgccca ccaagtgatt ggttaagccc atacggacag gcggggcagc tgccgattgc
    11881 ggtgagagac cgcattattg atttagttaa aagctaaatt ttataagtct tttgtttggg
    11941 aatacagcgt cccagaataa aacatcactt gtgaaatggt caataaagag taggattagc
    12001 aaatatttgc tggcgagagt gccaacgaca gactctggaa atagtttttg cggatgtaag
    12061 ataaatacat aaagtaaagc tatcataaga cgtttaaaga caggtgagga tttcacctgt
    12121 tttttttatg cggatatatt tatcgtatgc tatgatgcca gggagtgcaa cgcacagaac
    12181 aatccgtagg cattatagtt ggggactttt gacacccaca tcatgctatg attggagaaa
    12241 aagaatgaat cgaaacaaac agatgcatgc tccggtttat gaggcactgg agcgtttaaa
    12301 aagaagaaga gttgtgccgt ttgatgtacc gggacataaa cgcggaagag gaaatccgga
    12361 acttgtggat cttctgggag aaaaatgtgt cagcctggat gtcaattcca tgaagccgct
    12421 ggataacctc tgtcatcctg tttctgtgat caaggaagca gaagaactgg cagcggaagc
    12481 atttcgtgcg gatcatgcct tttttatggt tggcggaaca acttcttccg tgcagggaat
    12541 ggtgctttcc tgctgcaagg caggtgacaa gatcattctg ccgcgaaatg tacataaaag
    12601 cgtgatcaat gcactggtat tgtgcggtgc ggttccggtg tatgtgaatc cggaagtgga
    12661 tacaaaactg gggatatcac tcggaatgca ggtttctgag gtggaaaggg cgatccgtga
    12721 gaatccggat gcggtagcag ttcttgtgaa caatccgacc tactatggga tttgttctga
    12781 tctgcgtgcg atcgttaagg tggcacatga acataatatg ctggtgctgg cggatgaggc
    12841 acatggcacg catctgtatt ttggcagtga tcttccggtc tgtgccatgg atgcgggggc
    12901 agatatggct tcggtgtcca tgcataaatc cggcggaagc ctgacacaga gttctctgct
    12961 tcttaccgga aaaaatgtaa actgggaata tgtaagtcag attataaatc tgacacagac
    13021 aacaagtgct tcctatctgc tgatctccag tctggatatt tccaggagaa atctggcact
    13081 gcgcggacgg gagtcttttg ataaagtgag ccgcatggca gaatatgcaa gagaagagat
    13141 caattcaatc ggcggttttt atgcttacgg taaagatatg ataaacggcg gaagcgtcta
    13201 tgattttgat gtgacaaaac tttccgtgta tacaagagat atcgggcttg ccggcattga
    13261 ggtttatgac ctgctgcgtg atgagtatga catccagatc gagcttggag atatcgcaaa
    13321 tattctggcg tatatttcca tcggtgaccg tatccaggat atcgagcggc tggtgggggc
    13381 actggcggat atcaagcggc tttatgcaaa agatccggaa aaaatgccgg gagccgagta
    13441 catcaggccg accgttctgg tatctccgca gactgctttt tatgcagaaa agaaagcagt
    13501 tccgatccgg gagacaaaag gcatgatctg cagtgaattt gtgatgtgct atccgccggg
    13561 gatcccaatc cttgcaccgg gagaattgat cacgcaggag atcattgatt atattattta
    13621 cgcaaaagaa aagggctgtt ccatgcaggg aatgcaggat acagagatag aatatttata
    13681 tgtgctgagc gaaaaataaa agcaaatggg aggggaaaag gatggaactt tggttttcag
    13741 attaccagac agaaaaagta aaagtttcgg taaaagttca gaaacagctt tttggtctgc
    13801 agacggaatt tcaacgtatt gatgtgtttg attccgatga atttggccgt tttttaagtg
    13861 cagacggaag tattgtattt tcagaaaaag atgagtttat ttatgatgag atgatcgttc
    13921 atgtgccgat ggcagttcat ccatgtgtga aaaatgtcct ggtgatcggc ggcggggacg
    13981 gcggtgtggc aagagagctg agccattatg aagaaatcga aaaaattgat gtggtcgagc
    14041 cggaccgcgt ttttgttgaa gtctgtaaag aatttttccc ggacaatgcc tgcggactgg
    14101 cagataaaag agtcagtgtt tattatgagg acggactgaa atttctgcgt ctgcgtcata
    14161 atgaatatga tctgatcata aacgatgcga ttgatccgct cggacataat gccggactct
    14221 ttaccaaaga attttacggg aactgttacc gtgcactaaa agaagacggc atcatggtct
    14281 atcagcacgg aagtccgttt tatgatgaag acgaagaatc ctgccgggtg atgcaccgga
    14341 aggcaagcca cagtttcccg atcagccgtg tctatcaggc acacatcccg acctgttcat
    14401 ccgggtactg gctgtttggg tttgcatcaa aaaaatatca tccgctggaa cacctgaatg
    14461 caaaacgctg gaaagagcgg aagatagaaa cttggtacta cacgaccaat cttcataagg
    14521 gagcatttat gcttccaaaa tatgtagaag atatgctgga agaagaggaa ggtcgtaaaa
    14581 aataaaaagc aaaaagaaaa ggagattgta ctatgggaag attactgatc attggatgcg
    14641 gaggagttgc aggagttgcg atccacaaat gctgtcagaa cagcaaaaca ttttctgaga
    14701 tctgtattgc cagcagaaca aaggcaaagt gcgatgcact gaaagaaaaa ctggagaata
    14761 caacggaaac aaaaatcaca acagcacagg tggatgccga taaggtggaa gaactgatcg
    14821 cactgatcca gtcttatcag ccggatgctg ttttaaatgt ggcacttccg taccaggatc
    14881 tgacgatcat ggatgcgtgt ctggcatgca aggtggatta tattgatacc gccaattatg
    14941 agccggaaga taccgacgat aaacagtggc gtgaaattta cgagaaacgc tgcaaagagg
    15001 ccggatttac cgcatatttt gactattcct ggcagtgggc ttatcaggaa aaatttaaag
    15061 aagcaggcat cacggcactt ctcggaagcg gctttgatcc gggtgtcacc agtgttttta
    15121 cagcctatgc actgaaacat tattttgatg agatcgagac gatcgatatt ctggactgca
    15181 acggaggaga tcatggctat ccgtttgcca ccaactttaa cccggagatc aatctgcggg
    15241 aagtgtccgc aaacggttct tactgggaga atggtcactg ggtagagact gagccgatgg
    15301 agatcaaaag agaatataat ttcccacagg tcggcgaaaa agatatgtat ctgcttcatc
    15361 atgaagaaat cgaatctctg gcaaaaaatg ttccgggcgt aaaacgtatc cgtttcttta
    15421 tgacctttgg acagagttat ctgacgcaca tgaaatgtct ggaaaatgta ggcatgcttt
    15481 ccacttcacc gattctgttt gaaggaaaag agatcgtgcc gatccagttt ctgaaggcac
    15541 tgcttccgga tccggcttcc cttggaccgc gtaccgtcgg aaagacaaac attggctgta
    15601 tttttaccgg aaagaaagac ggaaaagaga agaccattta tatctacaat gtatgtgatc
    15661 atcaggagtg ttataaagaa gtcggatctc aggcgatttc ctacacaacc ggagtgccgg
    15721 cgatgatcgg ggcagcactt gttgtggata aagtatggga caaagacggt gtgttcaaca
    15781 tcgaagaatt tgatccggat ccgtttatgg atatgctgaa tcagtacgga ctgccgtggg
    15841 ttgtggatga aaatccgcag acagtagaat gatgtggaaa gatctgccga caccgtgcta
    15901 tgttgtggat gaaggcaaaa tcagaaaaaa tctggagatt cttgcggatc ttgagcagga
    15961 gtgcggctgt cacatcctgc tcgcccagaa agcattttct atgtacagcc tgtatccgat
    16021 gatcggtcag tatatcagcg gaacaacagc gagcggactg tatgaggcaa gacttggata
    16081 tgaagagatg ggaaaagaaa atcatgtctt tgcaccggca tacaaagaaa aagatatgga
    16141 agaactggtg cagatctgcg accatatcat cttcaattcc tttgcacagt atgagaaata
    16201 taaagaaatg tgccgggaaa gtgcaaaggt gagaaaagat aaaaaagcag tcagtgtcgg
    16261 catccgcatc aatccgcagt gctccacgca ggaagggcat gagatctatg atccttgtgg
    16321 ctatgcttcc agacttggtg tgacagaaga acagttccgg gaagatctgc tggagggtgt
    16381 ggaaggactt catttccaca cgctgtgcga acagaatgca gatgatcttg taacgacttt
    16441 tcaggcattt gaaaagaaat tcgggaaata tctgtaccgg atgaaatggc tgaatatggg
    16501 cggaggtcat catatcacaa ggggaaatta tcagatagaa actctgaaaa aactgatccg
    16561 ttatattaga gaaacctatc cggtggaagt ttatctggag cctggcgaag cgattgcttt
    16621 aaatgccggt tatcttgtga cagaagtgat ggacatcgtg cacaacgata tggacattct
    16681 gatcctcgat gcatcggcag cctgccatat gccggatgtg ctggagatgc cgtaccgccc
    16741 gccgcttgca aaaggatttg aagcagggga aaaacagtac acgtatcgcc tctcttcgat
    16801 gacctgtctt gccggggatg tgatcgggga ctacagtttt gagcagccga tccgtatcgg
    16861 ggatcggctg gtctttgaag atatggcaat ctattccatg gtaaaaaaca atacctttaa
    16921 cggaatgccg cttccgggaa ttgcactttt aaaagaagac ggaagtgtgg aaatggtaag
    16981 agaatttggc tatgacgatt ttaaaatgcg tctgtaattc aaatcgaacg catatgagac
    17041 ttgcaagcga cagcaccgta gattctgaag gaggatacga tggaatacaa cagaacaaca
    17101 ccgtctgcgg acggatatta tatgccggca gagtatgagc cgcataaagg ctgcatcatg
    17161 atctggccgc agcggccggg ttcctggaaa aaggatgcaa aagatgccga acaggctttt
    17221 tgtgcagtta tggaagcgat cacaaaaagt gagccagtgt atctggctgc cggaaaaaag
    17281 gttcttgcac gggcagaggc atttgccgca cagcagaaag aaaaatatag agaagccatc
    17341 gcatgcggaa gacattttcc ggtgaccgtc tttgaggcag aaacggatga tgcctgggca
    17401 agagatatgg caccgacgtt tgtaaaaaag ggaggcagcg ttcgggcagt aaactggtct
    17461 ttcaatgcct ggggcggaac ggtggatgga ctgtatgcat cctggaagct ggatgatgcg
    17521 tttgccctgg atttcgccaa aagagaagga tatttctgct acgatgcggc accttttgtg
    17581 ctggagggcg gttccatcca cagtgatggg gaagggacag tcatggtgac ggaagcctgt
    17641 ctgctcagca aaggcaggaa tccgcagctt tcaaaagaac agattgagga gaaactgaaa
    17701 gcatatctgg gtgcggagaa agtgctctgg ctgccaaggg gcatttacct ggatgagaca
    17761 aacgagcatg tagataatgt atgtgctttt gtcggtccgg ctgaggtggt gctggcgtgg
    17821 acggatgatc cgaaagatcc gcagtacccg ttatcactgg agacacttca gtatctggaa
    17881 ttacagaccg atgcgaaagg cagaaagatc atcgtacata aacttccgat cccggatgtg
    17941 ccggtctgcg ttacaccgga agatctggaa gggtatgtgt ttgaagaagg agaagataca
    18001 agagaagtgg gcgaacgcct tgcggcatct tatgtaaact tctatttttc aaacggagca
    18061 gttatcatgc cggcttttgg cggaaaaaat gcagaaagtg atcagcgggc agcagagatc
    18121 ttgcacagca tatgtccgca gagagagatc ctaccggtgt atgcaggaga tattctcacc
    18181 ggaggcggaa acatccactg tattacgcag cagataccac agggaaaggc agaaaaggaa
    18241 tgagaaaagt aaaaacagcg gcgatccaga tgcagtgcag cacagagctg caggaaaatc
    18301 tggaaaaagc agaaaagaaa atcagagaag cggcagcaga aggagcaaat attattttac
    18361 tgccggaact ttttgaacgg gaatatttct gtcagcagag acggtatgat ttttatcatt
    18421 atgcaaaacc ggcagaagaa aacgaagcag tacagatggg agtgcgtctg gcaaaagaac
    18481 ttggagtcgt gcttccgatc agcttttatg aaaaagaagt caacaatctt tacaattcga
    18541 tcgcatgcat cgatgcggat ggaaggattc ttgggattta tcgaaaaaca catatcccag
    18601 atgaccacta ttatcaggaa aaattttatt ttacgccggg cgataccgga tttcgtatct
    18661 ttgagaccag atatggaaag atcggcatcg gaatttgctg ggatcagtgg tttccggaga
    18721 gtgccagatg catggcactt cagggtgcag aacttctgtt ttatccgact gctatcggtt
    18781 cggagccaat tctggaatgt gacagcatgc cgcactggag gcgttgtatg cagggccatg
    18841 cggcagccaa tctgatgccg gtgatcgcag caaaccgcat tggaaaagag gtggtagagc
    18901 cggatacgga aaatggaggt cagaagtctg cactgacttt ttatggttct tccttcctga
    18961 ccgatgaaac aggcgaactg aaaaaaacag cttccagaga tcaggaagag attctgatcg
    19021 gagaatacga tctggatgag ctggcagcaa agcgtcttga atggggactg ttccgtgacc
    19081 gcagaccgga aatgtacagg atcattgcag gacaaaaata gaagaaagaa gacagatttg
    19141 ttgtttaaca tatacagaag aagagttttc tccaactaag ataagattta cgaggggtga
    19201 gcagatgaaa ttttttcatt tatccgatct gcatatcgga ctgaaactga tcgaacgcga
    19261 tctgcgggaa gaccaggaat atatttttga acagatcatc cggttggcaa aagagcagca
    19321 gccggatgcg atggtgatcg caggggatat ttatgacaaa gcggttccgt ctgcggaggc
    19381 agtggagatt tttgacagct ttatgaaaaa tctttccgaa gaaatgccgc agatgccggt
    19441 gatgatcatc agcggcaatc atgacagtgc cgccaggctg aactgttttc gcagtatttt
    19501 gtccaggcag aatgtctata tgattggcac gccgccgact acagaagagg aatatatcga
    19561 aaaagtgaca ctgcaggatg cttatggtga tctgcacttt tatctgcttc cgtttgtgaa
    19621 accgtcgatg gtaagagaga tcgtgggaac gcgggaggac ggaacacagc attcctacga
    19681 cgaggcactg catcttctga tcgaaagaga aaatatcgat acaaaggagc gaaatgtgct
    19741 ggtcagccat cagttttatc ttccgtccgg ccgacaggcg gaggacgtag agcgcatgga
    19801 ttccgagatc cgcacggtcg gaaatatcga tgagatctca gcagatgtgc tggaagtgtt
    19861 tgattatgcg gcacttggac atatccacaa accgatgaaa gtgggaagcg aatattattg
    19921 ttactgcgga acaccgcttg catgttcggt cagtgaagcc ggacagaaaa aaggcattgt
    19981 gatggtgacg atgaaagaaa aaggaaatat ttccacggaa gtgcttccgc ttccgccgct
    20041 tcgtcaggtg cgtgtgataa agggagaatt tcaggaggtg ctttcgcagg catgcgaaga
    20101 ttatgtcacg gttgtgctca cagacaaaga agatctcgat gtgatcgacc tcaaagaccg
    20161 tctgcgaaat gcatttccat atctgcttga gatccgcaga gagagggagc ggagagcaga
    20221 gtacaaaaga cagagcagaa agatcgaaga actggacccg tttgaacttt gctgttcgtt
    20281 tctcggggaa gatatggatg aggaagaacg tgagatgctg ctggatgtga tcagcagtgt
    20341 acagggggtg aaataagatg cggccgttaa aacttgtgat gcaggcgttt ggttcctatg
    20401 gaaaccgaac cgagattgat tttgaaaaac cggatcagaa tttattcctg atcaccggaa
    20461 ataccggctc cggaaaaaca acgatttttg atgcgattgt gtttgctctc tacggagaag
    20521 ccagctccaa tgcaaataag aaaaacggtg cggaacttca gagtcagttt gtggagatcg
    20581 ggacggagcc gtttgtctcc ctgacttttt ctgagaaaaa cggaactgag atcgattttt
    20641 ataccgtgaa acgcattccg cgtcatgtac gtccgctgaa acgcggcagc ggcgaaaaaa
    20701 tggtcagtga ggaagtttca cttaccatgc cggacggcag cgaatatccg gcaaaagaga
    20761 cggatgcgaa actgatggag attgtcggac tgacaaaaga gcagtttatg caggttgcga
    20821 tgatcgctca gggagaattc atggaacttc tgcgtgcaaa atcagatgat aaaaaaatga
    20881 tcttccgcaa gctgtttcac accggtctgt acgaggagat caaaaatgaa tttgccgtac
    20941 gcaaaaaaga aaaacaggca gagatcgatc agatccgcat gcggtgtatt gccgagatca
    21001 gccgcacaga acttccggaa ggtctggaac agacggcatt tttaaaagaa aagaaaaaag
    21061 aactggaaaa atcaaaagat ttttcggtaa cgatcctgga gcagtttctg gagaaactgg
    21121 aacttctgtg cgaggagctt tcgaagcgga cgaaagaagc aaaaaagatc gtgcaaaaga
    21181 gaagtcagga acgtgatcat gcaagggatg ccgctgcgaa agcttctcag cttctgaaat
    21241 cttttgtaca gctggaagat gcagaaaaga ccttgcagag cttaaaagag caggaagaag
    21301 cgatcacaaa acagcaggaa ctggcggttc gggtcgaaga cgcctgggag ttacaggcag
    21361 tttatcagcg gtttttggac tgcaagaaaa tgctggaaaa attgagatca gaccttcagg
    21421 aacagacaga acagcttccg aaccttatgg aagatacaaa actctgtata cagaatacaa
    21481 aagatgcaaa acatcagcag gaagaagaag ttgcggcttt taccaaagtg gaagaaaaag
    21541 tgaaaaaagc catggaagtc tttacaaaaa tggaagctgc cgggcaggat gtgattcaaa
    21601 aagaaaaagc actccaaaac gcagaagaaa aagagcgagc actcaaagca aaaaaagagc
    21661 aacttgaaaa acagcaggaa acgtggcggg aacgctcaca gcagcttg
//
LOCUS       DAWDWH010000012        20199 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_1592_length_20199_cov_13.411338, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000012 DAWDWH010000000
VERSION     DAWDWH010000012.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 20199)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20199)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20199
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1592_length_20199_cov_13.411338"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6231..6240
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10369..10378
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15273..15282
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19075..19084
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4340 a         5341 c         3841 g         6637 t
ORIGIN      
        1 cattcttctt tctacagcat tacgtcatgc tctagccatt ttctttaatc cttttccata
       61 aatgctattt cttacaaatc ccacctttga catacaatta tgtctctggt atttcttgtt
      121 ataccatctt gtaaaagtgc aaataatgta gcagtctagt gccgcatcca cttttcgttt
      181 ctcttggcgg aatgatacaa ttgctcgccc ctttttcaag gttactttat ccactcgctt
      241 agcaccctgt attactacac ggctcactgg cgattatcgt gaccggactt acactggcaa
      301 gtgtagtcca gctttgctgg agacacgcat aaaaaaagta cagaaacaat tcccacgtct
      361 ctgtactttt tcacatcttt tatttgcttt cttctgcctt ttctacttca gcgattgctg
      421 attttttcat tggaatacgg cagtttttgt tgcttccgaa ttctacgatc acgtcttcgt
      481 ctgtcagatc gatcacaact ccgtaaaatc cgcttgttgt aacaacgctg tcacctactg
      541 caaggcttgc cagcattgtg tccagtcttt tctgttcctt tttctgcggt cttaccagca
      601 ggaaccacat aactgcacag atcaccacga tgtagatcag ggtgaatccc agtccgcctg
      661 ctgttgaaga tgtacttaat aaattcatat ttacctcctg gtcatgcgta tttcgcttaa
      721 atttcgggct ttcccgaaaa agtgtcacta acctatatca tacacaatcc ttgcctgtta
      781 ttcaagcatt attttcaaat cctgacagtt tttctttttt aaatgcctta taattgccgg
      841 cttcaatggc atctcggatt tcttccatca gatgattgta aaaatacagg ttatgcagca
      901 cacacagacg catgcccagc atttcttttg ctttcagcag atgtcggata tacgccctac
      961 tgtagtgacg gcatgccgga cactggcatc cttcttcaat cggcttcgga tccagctcat
     1021 attttgcatt gaacagattc agctttccat gattcgtgta gacatggcca tggcggccgt
     1081 ttctgcttgg ataaacacag tcaaagaaat caactccccg gtctactgct tccagaatat
     1141 ttgccggtgt accaaccccc atcagataag ttggcttatc caccggaagc tccggtacga
     1201 ctgcatccag aatacggtac atctcctcat ggctctcacc gactgccagg ccgccgactg
     1261 catatccgtc cagatccagc tcacggatct gctgtgcatg ctcgatacgg atatcttcgt
     1321 agatcgcacc ctggttaata ccaaagagca tctgatgctg attgatcgta tccggcaggc
     1381 tgttcagtct tgccatctct tctttgcagc gatgcagcca tcttgtcgta cgctctaccg
     1441 aacgctggac atattctctt tctgccacac tggacggaca ctcatcaaat gccatggcga
     1501 tcgtggacgc cagattggac tggatctgca tgctctgttc cggtcccatg aaaatctttc
     1561 tgccgtctac atgggaattg aaatatacgc cttcttcttt gatctttcga agacccgcca
     1621 gcgaaaatac ctgaaatcca ccggagtctg tcaggatcgg tttgtcccat accataaatt
     1681 tatgcagtcc gcccatcttt tttacaacct gatcacccgg tcttacgtga aggtgatagg
     1741 tgttggacag ctctacctgt gtgccgattt gctgcagatc ctcggtcgat actgctccct
     1801 tgatcgctgc tgctgttccg acattcataa aaacaggggt ttgtatcacg ccatgcacgg
     1861 tgtgaaactc accccttttt gccttgccat ctctttttat cagtttatac atgatttcct
     1921 ccaatttcat tcgaatgcat acaaactttg gtgtatcctt acgtctgttc aagtattata
     1981 gccctatttg gaggattttt caagtacttc accggggaat catccccggt gtcccgctaa
     2041 aggttggatt ggctgaccac catataaata gaagcagaac tctttccggt ctggaaatat
     2101 ccggcaggat tacatatctt ctgttaaaat cccatgatca cgaagcagtt tctcaatgac
     2161 ggttcctttt accactttga tgaccggttt tttcagtgcg ttcagcggtc caggcagctg
     2221 gatctccagc ggtgatttgc catccatcag tttgacggaa ctgtcaagca gttcacggat
     2281 atcgtaaacg cttccccata cttccggtac gccgcggtcc acaccaagca gtccgtaaac
     2341 agcctccatt gcggttctca ctgaatactc ggttgtgaag acggtatcac gcggagtctc
     2401 tgcgaactgt ccgaggaatg cgaagtttgt acatccatcc gggatgacat ccggtctgtc
     2461 acctttggtt cttggcatga agaatgcagt gatatacggc atcatcgtcg gtacgcagac
     2521 agcactgttc tctgccagtt cctcgatctt gtcggttgga acgcccaggt gatacagcca
     2581 ttcttcggta atctcttttc cggtacattc tttcatcggt ttcttgatat aatcgcccgg
     2641 cacatccgta aacagtccat atacccatac gcagactttt tctttatcct gctctttgaa
     2701 ctgtccctga cggttgattg tccagctgag cagccacttg gagtcctgac agcttacaat
     2761 tcctccggtt accacttttc cgcttctcgg atcacgtttg cagatgtttg tgatatacgg
     2821 aatgatcttg tcatccaaag tcgtgatcgt tgctgactcc cagtttgtct tctcgatatc
     2881 cgagcagaat ttctccggat gtccaaagga cggatcctgt tttgcaatgt ttttccacag
     2941 atcccagcag ccgcttctgc gtacttctgc atctgcatcc ggtgcatggt tctgatctcc
     3001 gtaaatggtt ccttccgtac agcttccgtt tgtgacaaag acaaggtcat cttctgtcag
     3061 gagaatattg tcttcttctc cgttcacttt gcattcaatc gatgttgcga tctttttgcc
     3121 ttctttaaag gtaaatacaa cgtttgtcac ttcggtgttg aactggaaat ctacgcctgc
     3181 ttcttccaga tatttctgca tcggcaggat cagggattca tactgattgt atttggtaaa
     3241 tttcaaagca ctgaaatccg gcagtccggc gatgtgatga ataaatctct ggaagtagag
     3301 cttcatttcc agtgcactgt gccagttttc aaatgcaaac atcgttctcc agtacagcca
     3361 gaaggtggaa tcaaacactt cgtcatcaaa tacatcttcg attgttttat cataaagatc
     3421 ttcgtctttt gtcatgaaca gtttcatgat ctccatgcag cctttctggc ttaaattgaa
     3481 cttgccatcg gtatgtgcat cttctccacg attcacagtt gctctgcaaa gagagtagtt
     3541 cggatctttt ttgttcagcc agtaatattc atccagaaca gatacgcccg gtgtttcgat
     3601 ggacggaatg ctgcggaaca gatcccacag gcactcgaaa tgattctcca tctctctgcc
     3661 gcctcgcatc acataaccgc gggacggatc aaagataccg tcacaggccc ctccggcgat
     3721 atccatcgct tccagaatgt ggatatggga tcctttcatc tgcccgtctc ttaccagaaa
     3781 gcaggctgct gccagagatg ccagtccgct tccgacaatg taagcatttt tgccatcgac
     3841 accttccggt ttttccggtc ttgcaaaagc ttcgtagtta ccgttgctgt aataaatacc
     3901 tttgctgttc ttttcatatt ttccacgctc ggtattgcgg taatcttttc tcttgtcttc
     3961 tctttttttg cttatctctt tctgtttatg ctcattttct tttgttttct tactcagaag
     4021 catcgctgct ccggctccgg ctgctgctgc catcgtatat tttaaccctt ttttcatgtt
     4081 gaatgcccac ctttccaatg tttgaggcta accttcctgc ctccgccatc ctgtcttcgt
     4141 tttctttgga aatttcctgt ttcaatccag ttctttctga attgcctaca ttctaacatt
     4201 agtatgcccg ggattctatt gacaaaacag gccagatgat aaaatttttg tcatctgacc
     4261 tgttttgtaa gctttttgct gtgattttct accttacttg ttgcagtaca aaacttcact
     4321 gatatttatc tgcggatgga ctgtgccatt ctgtgatact gatggcggat tccgcggatc
     4381 acagttccgt acgccataat attttccggc agtgccattc ccacgtatcc ggaatcaccg
     4441 atgtggtgga tgtcggttcc tgccatcttg cactccagtg cgatgcgttt gatcgtatcg
     4501 gtgtccgcac cttcctgaga agttccgatc gatgtgatcg tcagcttttt caggctgtgt
     4561 gcataactga tcagtttccg cacatattcg gtcgtgatgc caggcacggt tcccggtgcc
     4621 ggcagcagaa tgatatcggc tcctgcctct gcaaactggc ggatatcttc cttcgtgatg
     4681 atgttctcgc cgccttcgcc gatgataccg gaggcatgca ttttcccggc tgccagcatg
     4741 atcttctctc cgactgcctc tgagatcgct ttcagggaag aaatgatcgc cggattgctc
     4801 actccatttc ccggattgcc ggtcagaacg atcaaatcca cgcccatttt ctgagcctga
     4861 atggcatttt ccaccgttgc ataacgtccg gcactcatct cccacagggt ctctttgtta
     4921 tcggaagcat agctttcttc caccggttcc agattgatgc cgaccatacg tccggtcaga
     4981 cgttttactt ctcgtaccgt ctcttctttt tccacttccg gcagtgccat gatttttggc
     5041 tcctgcacat caaacatgtt cagaaggatc aggtctgctc ccatggaagc tgcaaactct
     5101 gcattagtca catcggtaag cagaggaatc gtgatgccga tcgtctcact tgcaagaacc
     5161 cttccctcgc tgcctgcgat tgcttcgatc agttcacttt ttgttgcatg cgtcatttct
     5221 tctgctgtca tattcaaaat acgttttgcc atatcagatt ctccttcttg ctttgctgat
     5281 aaaaagcgaa aaaaatctgc ccgaagacag attttcttct atcatgttcc atacagtttc
     5341 ctgtattcga cttgaatctt gttcttaatc cggcatatcg cctgcttctc cggaagactc
     5401 gccaagtgtg atccacttca ggtaagcatt gataaacagg tcaataccgc cgtccatcac
     5461 gctgtccaca tttcctgttt ctgcatttgt tctgtggtct tttaccatcg tatacggctg
     5521 catgacatag gaacggatct ggtttcccca gccaatctcc ttgacttcac cgcggatacc
     5581 ggaaagcttc tctgcattct cctgctgctg cagcagatac aactttgctt tcagcatctg
     5641 catcgctttg tctttgttca tgtgctggga acgctcattc tggcactgaa caacgatgtt
     5701 ggtcggcaga tgggtgatac ggatcgccga ggaggtcttg ttgatatgct gaccgccggc
     5761 tccgctggaa cggtaagtat cgatacgcag atcttcatcc cggatctcga tatcgatgtc
     5821 ttcctcaata tccggcatta catcgcagga tacaaacgag gtctgtctct ttccagccgc
     5881 attgaacggt gagatacgga ccagtctgtg gacacccttt tcggatttga gatagccgta
     5941 ggcattttct ccgttaacct ggaaggtaac ggacttgatg cccgcctcat cgccatccag
     6001 ataatccagc acatccactg caaaaccatg tttgtccgcc catctggtat acatacggta
     6061 cagcatgccc gcccagtcgc aggactctgt tccgcctgct cccgcatgga gcgtcacgat
     6121 cgcattatct ttatcgtatt ctccggataa cagtgttttt atacggatat catcataagt
     6181 tttcctgaaa tcatccagaa gatcctgaat ctctggtatc agagattcat nnnnnnnnnn
     6241 gctgccatct cgatcagagt ttcgatctca tcaaactcat cttttaactt ggcataaatg
     6301 gcgatatcgt ctttcatgct ctttaaattt ttcatggatt cctgtgattt ttccgggtta
     6361 tcccagaaat caggagcttc catctctctt tctaattcct ggatacgctg ctctttgtta
     6421 gccaggtcaa agtgaatccc tcacttccac taatggttcc ttaaaattgt ttaattcata
     6481 cttaaactga tctaattcaa ccacttgctt cacctctttc ttttttattt tcagtcactg
     6541 cgaggtgttt atttctttct tccgcagcac tgtttatatt tctttccgct tccgcatggg
     6601 cacggatcgt ttggataaat cttcttttct tcacgtttct gaggtgcatt ctgtacggaa
     6661 tcatctttgt ttgttccggt tacttttgca acttcttcac gttctacttt ctgttccatg
     6721 cttacatggt acagaagctt cagagtgtct tcctggattg ccgcagacat tgcatcgaac
     6781 atctcgtaag cactcatctt gtattctacc agaggatctt tctgaccgta agcctgaagt
     6841 ccgataccct gacgcagctg atccatatcg tcgatatgat ccatccactt gcggtcgatg
     6901 actttcagaa ggatcactcg ctccagttca cggatctgtt ccggttccgg gaactctgct
     6961 tctttcatct catacagctt cacagcctgc tctttgagca tctgtttgag ctcatttttc
     7021 ttcattttct gtgaaatctc aatcttcggc agcggaatga tcggaatcag aagtccgttc
     7081 agttcctgaa gattccattc tgaagtatcc tgatcatcag aaatgcacat atcaacggta
     7141 ttgtccacga tgtctgtgat catcttgaag atagaatcac gcatactctc tccgtccaga
     7201 acacggcgac gctctttgta aatgatttca cgctgctcgt tgttgacctg gtcgtactcc
     7261 agcaggtttt tacggatacc gtagttgttt ccttcgatct tcatctgtgc tttctcgatt
     7321 gcactggaaa gcattttgtg ctcgatctgc tcgttttctg caacaccgat ggatttaaag
     7381 atgctcatca gacgctcgga accgaacaga cgcatcaggt catcttccag cgaaatatag
     7441 aatctggatt ctcccgggtc accctgtcgt ccggaacgtc cgcgcagctg attgtcgata
     7501 cgtctggatt catgacgctc ggtaccgatg atcttcagac cgccggcagc ctttgcctca
     7561 tcatccagct tgatatcggt accacggccg gccatgttcg ttgcgatcgt aacggctccg
     7621 tgacgacctg cctctgctac gatttcagcc tccagctcat ggaatttcgc attgaggact
     7681 ttgtgcgaaa tgccgcgtct ctttaacata ccgctcagaa gttcggatgt ctcaatggtg
     7741 atggtaccaa ccagaaccgg ctgtcccttt tcatgggctt tttcaatttc atccacaacg
     7801 gcatggaatt tttctttttt ggtcatgtat accgcatcat ccaggtcttt acggagaacc
     7861 ggtctgttgg tagggatttc aactacgtcc attccgtaga tatcacggaa ctctttttct
     7921 tcggtaagtg cagtaccggt catgccggct ttctttttat atttattgaa gaagttctgg
     7981 aatgtgatcg ttgcaagtgt cttgctctca cgttttacct ttacatgctc ttttgcctcg
     8041 attgcctgat gcaggccatc cgagtagcgt cttcccggca tgatacgtcc ggtaaactcg
     8101 tctacgatca gtacttcatc atcttttact acataatcct gatcacggtg catcagactg
     8161 tgtgcacgca gtgccaggtt gatattgtgt tggatctcca ggttctccgg atcggaaagg
     8221 ttatcgatct ggaagaactg ttctactttc ttaacaccgt cctgtgtcag tgttacgact
     8281 ttatcttttt catttacgat atagtcaccg gtctctgtga tctcctcgcc cataatggcc
     8341 gacattttag tcacttcacc gctggcttca ccacgctcca gctgttttgc cagaatgtcg
     8401 cagacttcgt acagttttgt ggacttgccg ctctgaccgg aaatgatcag cggtgtacgt
     8461 gcctcgtcga tcaggactga gtccacctcg tcgatgatcg cataatgcag atctctctga
     8521 acgagctgtt ctttataaat aaccatgtta tcacgcagat aatcgaaacc gtcctcgttg
     8581 ttggtgatgt aagtgatatc gcagttatac tgtttacgtc tctcttcact gtccatggaa
     8641 ttcagtacac atccaacggt cagaccaaga aactcatgga cttttcccat ccactcagaa
     8701 tctcgacgtg ccagataatc attgaccgtt actacatgaa ctccttttcc ttccagagcg
     8761 ttcagatatg ccggtaaggt agatacaagt gttttacctt cacctgttct catctctgcg
     8821 atacgcccct gatgcagaat gatacctccg atcagctgta cacggtaatg ttccatattc
     8881 agtacgcgtc gtgcagattc acgaacggta gcgtatgcct caggaagcag atcgtccaga
     8941 gtctctcctt ctgccagtct ttttttgtat tctgctgttt tgcctctcag ttcttcgtca
     9001 gagagctcta tcattgtcgg acgcagtttt tcaatttttt caaccagcgg cataattcgc
     9061 tttacttcac gttcactatg cgtaccgaac attttctgaa acacattcat tgtctatatt
     9121 ctcctatcac attaattacc ttaaaaaaca gttccattct atcactttat cacttcatag
     9181 acaattaaat ttttgtaaat aacctatgaa cagcggataa gaacttaaaa agttgccgca
     9241 gacatgcgtt caccaaaaaa tctacggctg aactgtgcat cctgcggcag ctattgattt
     9301 attttactgt tttatttcag atggtgtagc tcttttatcc ggtcgatcct agcactccgg
     9361 ctcgatcagt ccatatgtat ttccttttct cttatacaca acgttgactt cgtctgtatc
     9421 tgcattacgg aatacaaaga aactgtgtcc gagcagttcc atctgtacac atgcatcttc
     9481 cgggaacatc ggtttcatac cgaactgttt tgtacggatg atctttactt cctcttcttc
     9541 accttcgttc tctttttcga tgaattcttt gcggaggttt cctccgtctt tatgtttgcc
     9601 tataattttt gtcttatatt tacgaagctg tctttcaatg acttcttcca caagatcaat
     9661 agaaacatac atatcattgc ttacctgctc tgagcggatg atatttcctt ttaccggaat
     9721 ggttacttcg attttctgtc tgtctttttc cacgctcaaa gttacaataa tctctgtctc
     9781 aggagtgaaa tatttttcaa gctttccaag cttttctgtg actgcacttc ttaacccctc
     9841 tgtcacttcg atattttttc cacgaattgt aaaacgcatg atacccaact cctttgcatc
     9901 tatatttgca gttgaaactg ctatgtgaac attatagcaa ttttccacaa tattttcaat
     9961 cacttttgca aagtttttag tttttttata ctatcttatg aattctcatc caaattggcg
    10021 gcacacacac gcagaacttc ccggtaaggc atgcttcccc caaagagatc cagggcactt
    10081 cctatcgtaa catcgatgtg atctctgcca agttttttca gttcctgaag atccgcaaaa
    10141 cttcccacgc caccggcata ggtgaccggt atgccctgcc atcttccgag catgtctgcc
    10201 agttctcttt cgattccctg tgcttttcct tctgcatcca ctgcatgcac cagaaattca
    10261 tcacagcagc cggaaagttc gtcaagtacc ttctcgctta actttacatc ggtaaacttc
    10321 tgccagcggt ctgtgacgat agagtatccg tcttcttttc tgcggcagnn nnnnnnnnat
    10381 gccgcctcca acctgcagtc ctcctggata cgtccgaagt gccagcatgg cctgctcttt
    10441 tgttttttca taaaacggag aatcttttgc attcagcaaa atcacatgtc cgccgcttat
    10501 ccggtctttc tgatacatct ctgcaaaaaa tgccgcatcc tgtgtagaca caaaattttc
    10561 ctttgcctga tctccttcgt ctcttaagct tcctcctacg atctgtttta cttttccgtt
    10621 atgtatatca atgcatggtc gaaatctcat acgtttctcc tctttttctg ttattcagat
    10681 ccgactctcg aaaactgacc ctcgattcct gtcgtctctt tgcatgttcc tgtcaaaatt
    10741 gctatgcatc tgcacgctgg tctgatcatt gtacccacac agaacctctg acgttttcat
    10801 tatagaaaag cctgttttct ttgtccagca gatattctcc agatttttga gaattgcttt
    10861 gtagcaaaat aattttcgca catctggctg taaatccaaa aaagaagcca cagaggcagc
    10921 gtttctgccc ccatgactta cctcagcgaa tcctcatctt tactcggtca tacgactgcg
    10981 gccggttgtc tcaccggtca ggtcatcaac cacatctccg actgcatcac ctgcatcacg
    11041 gacgatatcg cctgcatcat cgagtactcc atctgctccg tctgtgcggc gtgactgtga
    11101 agtgtccgac tggtttctgt tcttttttgt gctgttattt ttctgtgtgt tgctttctgc
    11161 ctgtgtaccg ctgcttccgt tctgcatatt gctttccgcc tgtgtaccat tgcttccgtt
    11221 ctgcatattg ctttcgcttc cgctcgttgc cgatgtattt ccgtttcggt tgctgcatcc
    11281 tgtgataccg gaaagtgcac agacaattcc aaggactgcc agcatgcttc ttgttcttct
    11341 catagtcttc tccttttctt tgctttttcg gagcttctta tgcccatcct taatatgcat
    11401 cctgtgtact ttttttattc atgttccttc gtgtcgattt tgattttcaa agcacaccca
    11461 ttgttatctt cattattctt tcaggttgat attaaaccgg attctttttc ggaaaatcct
    11521 tctgcaaaac tgttttccgt caaatgggat cagcggatag agataacttt ctccggatac
    11581 tgtacggttc agaagcagac ttaagaacgt gattaccaga cctgccgcat atccggcaag
    11641 tccgaaaacc gccgtcagga tcagcagcag aatccgcata aattttaggg cgtatcccag
    11701 ttcatagctt gcctgcgtgt aatttgcgat tgccacaaat gccatatata acatcgtctc
    11761 tgcattaaac cacccggatt ggactgcaaa ttcaccgaca acaagaccgg cgatcacact
    11821 gagtggcgtt gtcagcatac ccggcgtatt aatcgctgcc agtttcagac cgtcgattgc
    11881 aaattccagg atcagcagct gtgcaaagac cggaacatag agatctccgc ttattttaat
    11941 aaatgcaaac tgcggtggta tccactgcgg attctgtaaa aaaagcagcc aggtcggggt
    12001 cagaagcagt gtcagcagtg ctatgacaaa ccttgtaaca cgaagatacg tgccggtcac
    12061 cggcggaaaa taaaaatcat cggcttcttc cataatgtca aacaccgata ccggaaggat
    12121 catgactgcc ggagagttat ccgttaaaag cacaattttc ccatccagga tctcagctgc
    12181 tgccgtatcc ggacgctccg tatatttaaa tttcggaaat ggattgtacc acttatgtgg
    12241 ataaagacat tctgcaagac tctcctggtt cattgccagg gcatccgttc tgatctgctc
    12301 cagtcttttt tcgatctgct gcaagaatcc ggaatccaca cgatcttcca tatagcacag
    12361 caccacatcg gtatgcgaag tgcttccgac atttttcatc tccatggaca gtttcggatt
    12421 gcggattctt cttcggatca gtgctgtatt ggatacgatg gtttccacaa aaccatccct
    12481 tggtcctcgc agtactttgt ctttgtccgg ctcttcgaca cttcgcatcg gataggtacg
    12541 acagtccata gcaaagcatc tggaataccc gtcaataaac agacacatca cgcccgataa
    12601 aatctgcgtt gtgatctgct cacagtcctc taaaagttca acttctacat agggcatggc
    12661 atctcttgac agctcttcgc tgtcttccgg cagatcttcc ggggtcacag aataaaagaa
    12721 ttccagtaac ttttccacaa tgctctcttt taaaaatccg tccacaaagt aatagactgc
    12781 ttttcggtct gctaccatcg tcgttctgct gatgatgtca aaatttttgt ccgtccgcag
    12841 aatccggcgg aaatatttct gattttcttc caggcttgtc gatagcttct gctgcacatt
    12901 tttcacctct tcttattact cactatcgtg attatttcca tttttctgtg ttttattctt
    12961 ttagactcca ttcttccagt catacttatc tgaaatccgg cagtcgattc cgggctgttt
    13021 tactgatatc tacttatttt tatctgatat gtattttctt tctgaaaaac cataatatca
    13081 tgctcgatgc tgccatcaga cctgcgaaga tccacatttc catccaggat gcatcacctg
    13141 ttttggctgc tgtcacagcg ttttcattat ccgatgtatt atctgatgaa aatcctgaat
    13201 ggtcgtttcc ggttgtttta tcaaagttct cggtatccac acgtttttct gtctgcacct
    13261 gtgcttcttt cgtcagttcc actttgtatt tctggacatt tgcaactgct ttgactttaa
    13321 tcttttgatt tttcggtttc tgataacctt ttggcacttt ctcgacctga atggtatacc
    13381 aggatgggat tactttttct ttcaaagcag taagtgtcac atgtttccag attttctttc
    13441 ctttttcatt aaccacactg atctttaccc cttccagtgg tgtctgcgtc tttttgtcaa
    13501 cggcttcgat tttaatcact gtcggacgga tctctacctc aaaatcctgt atttctgacg
    13561 tatcttctct taccgtgatc ttcgtctgtg ccggaagtac atagccatcc ggtacacggt
    13621 ctgttttcac ctgataactg ccagacggaa cacgtgtata ggcttccgga agttccagtg
    13681 accgctcctc aaacagatct ctgatatgta cataaatccc agaccggatc gttcttgtct
    13741 gcttcttttc tgctgctgaa gacaatgcat agccggtaat ccggatacag attggttcca
    13801 ccagaacttc aaatttctgt acttccgctg tatctttcac ctcaatgtct atcacatccg
    13861 gatgtacata acctttcgga atttcatcca ctacgatctt atagtgcccg atcgccactt
    13921 ttttaaagat ttctgcctgt tctgttccgg actcccagat gccgtcccgt tccatcggac
    13981 tgttttcttt caggagcgtc tcgccctgcg gtgtctccag gtgagctttt acatttccaa
    14041 gcggaacttt tttatctccg tctttttcaa agacagtgat atgaacttcc gtcggcgggt
    14101 cctgcatttc cagtttctgt ccctgataag tgccatcttc ctctttatga tcttgcatct
    14161 gaaatacctg ttctgctgct gttgcatagc caggtgccgg tgaaagttcc cgcaaggtat
    14221 aggtttttcc gacttccagc ccttccacgc aatgctcttc tttctctgtg atccagcttt
    14281 cgatacaggt attttcctga taaatggcca gttttgctcc ttctaacagt tcttttgtat
    14341 cggatgccgt cttcttcaca aacacttttg tgatctgatt ttcaaagata acctggaatt
    14401 tctgtactgc catcccgtct tctttccagg atgcatcaat ctcttctgtc tgtgctgtcc
    14461 ggatatagcc atccggtgcc gcaatctcac ggacttcata ctttccaagt ggcaggtcgc
    14521 tgacaaacaa caggtttcct tctttatcac ttttctgtgt ttccagtaaa gttccttttt
    14581 ccagaagaac ttctcctttt gctgttttga tatcctctgc tgcatacagt ccgaaaactg
    14641 caccttccag cattttctgt ggattttctt tatcgcattt tgttacccgg atctccactt
    14701 tctggcgttc gttgtatacc gggacttctg cttctgtgac atctgcttca cttccatctg
    14761 acgtttctgc cagcacaaac gaaaccttat cttttttctt ctgatatcct tccgggatat
    14821 ttatctcttc cagctcgtat gtttcaagat acatctgttc aaacaccaga cttccatcct
    14881 gatcagtatc tccttctgct ttcgcagtct tctgacttcg taccagatac ggaacaacct
    14941 ctcccgcttt gcagatcaga ccggtcacgc catccgggtg atacagatct tccttcgctt
    15001 ttactgtaaa gcttacacct tttacgcttc ctgtttgata accaaaccag gtttttatct
    15061 ggttccataa tccctgtaca attccaagct ttctggatac ttcgtttaag atctctcctg
    15121 tctttgaaat tttcaccttt ccggtcatga aagcattttc cagattgaaa actacttctt
    15181 ccggcatctg ctccatcgtt tcctctgtgt actgctgtga aacagaaaaa gtaacctcct
    15241 gtttctgctc ctgtttcaga atgtctttca tgnnnnnnnn nnaggatttc ttttttatag
    15301 ccttttctcg gaagaacttc cacgatgcgg tacgtttttt ccggattcag ccccaggatc
    15361 agacagccct ttccttccgt attttctgtg ctgactgcat gcagaatttt tccatcttca
    15421 tcgagaatct gaagtttcgc acctgccaac tcttttttcg tgagatcatc tctcagaaac
    15481 agtcttactt ttactgccgc attttcgatc tcgctcttca tacggatgat aaaaatgtga
    15541 tcctggtctt tatacggttc aaaatcaaaa atccgctccg tatcagatcg ataatatcca
    15601 tccggtgctt ttgtttcttt tactgcatag gtgccaggcg gcagatctgt ttgaaaaacg
    15661 gctcttccgc tctgatctga aatggcacgc tctatctccg ttcctgccgg cagcacaaca
    15721 cttccgtttg cgtccgtaat atcttttttc gcataaagtg tgaattctgc tccttccggc
    15781 ctgttttctt tctctccgtc cacacatttc ttaaagacac cggtccttcc tttccactct
    15841 tctccgatga tcgttcccgg ctcttccggt accgtcggag acacctccgg gccatccggt
    15901 tcctgcccga tatcaatctt ctgtctttcg ttgaaaaagg tttctgttgc ggtaatttcc
    15961 agatctgcac tgtctttcca caacagttct gtgtttcgtt ttgcatcttt ctgctttcgc
    16021 gtgtagccgg acggagcttt tgtctctctg atctcatacc ttccaagcgg cagatccgaa
    16081 agaaccgctt ttccctgttc atcggttatc acatggacaa tggtcgttcc catcagataa
    16141 aggtctttgc tgtaaccgtc cggatgcgga atcgtttctg ctgccacaag gtcatattct
    16201 gcccctttta acccttttgt ctcatagact atcttctgca gtttctgatc tttgatctcc
    16261 gcttccggca cttctccttc ttttcggatc tccagttttc cggaaatgga acggttaaac
    16321 aggctgacaa cctgtacaca ttctttgtct tccagccgaa tctctgcctg attttcctga
    16381 caccgaaata ccttacttgc gattccctca aagaaacgat ttccctgttc gtatccttct
    16441 tttacatgcg gggacttata accttctttt tccagaatct gccagttata tccttctctt
    16501 gcctttgtct ccctgatctg ataagtttct ccgactttca gcccccggat cagcacattg
    16561 ttgccatgta tactgactgc ttcctgcaca atctcaccct tcgaatccac gatactgtat
    16621 tttactccat ccagctccgt ttctgtatca tagtccatga tattaatctg aagcattgtt
    16681 ttatagtttt caaacgtgcg tacatacgat ttgattgtag catcatgcgg tgtgcctgtt
    16741 ttggcatttg atttcctgtc attttctgtc ctgtttgcca tattatcttc ggatctgcca
    16801 tcttctgatg catttttgct gtcatcttca gctgtaaagg aaatttcttc tgtattatca
    16861 gaccgataat atccatccgg tgctttttct tctctgacat aatagtgtgc catcggaaga
    16921 tccaggtcaa aggccgcatc cataataccg ttttccgctt cttttgtctc tgcgatcgcc
    16981 agtctggtat ctttctttac caggatgctt ttctgatcag ctgccaaaat atcttctgcc
    17041 gcataaagcc caaactttgc tcctgtaagt ttttcttttg tttctgcatc tttcttttca
    17101 atctggatct gtgctctctg tcttggaatt tcatacagtt cactctgata ccggattgga
    17161 acttcctgtc cggcatatcg gatctcaaat gttcttgccg cctgattttc cccggaaagt
    17221 gcatagcctt tggctgcttt tatctgttcc agcgtataag tgccgagcgg aagtccatac
    17281 cattcccaac tggctgcttt tattttttca gtccatgctt ttccattttc atcggtagtg
    17341 agtgtggtca caacacttcc ttttgcaaaa agtaaagtct gtcctccctt tccttcaccc
    17401 gaaaagatat cttctgctgc ccgcacgaca aagacattgc cggcaagcgg cttacaggta
    17461 taggcaaatc cctttttcag atcgtatccg gtcagtactt ctccctctgc ataaatgctc
    17521 agtttgccga ctgccgcctc gttttcctga accgcttccg tcacaaaggc tcctgccagt
    17581 ctgtcatacg ttgtctttgc ctcatccgaa ccgatctgca cccgcacccg gtaatcttta
    17641 gaagcaatgt ctgcctgttc ccaggttccg accgcttctt ctgtgatttt cagtgcgtgg
    17701 accgcttcca tacccggcag cttttttgcc gaagcttcaa agaaatactg tccgtttctt
    17761 ttgcggtatc ttccttctgc tccctggcgt acataacctt ccggtgcctg cacttcttct
    17821 aaaatataaa gtccctgcgg cagtgttgtg gtcgtggaga cataagtgcc gagcactctg
    17881 ccgtcgctgt cctttcgctc tgcggtcaca taaggatttt cataagatcc cagcatctct
    17941 ccgtttgtat aggcaacgac aagatttcca aagcgattga tataatcgac tttttcctca
    18001 ctggtcttat cttccatata ccagttaaag taggctcctt cgacatcatg gatcacatac
    18061 tttgctcccg gtttcgctac cgtttccccg gttgttttgt ctttctttat gattttaaga
    18121 taactggtct gcggctgatc ccagatggca gatcccatct gcaatgtccc tttgccgtcg
    18181 ccgtccacca gtccgtcttt ttcatccgaa tcgacgttca gcactttcgg tgccgccgcc
    18241 acctttcctt ctggaacggt cgtctcgacc agtagatatt tgccatatgg aagtgcccgg
    18301 atattcagaa ctccgttttc atcggaaaac tgctccttaa gacgctgcat cttacctgcc
    18361 tcatcataat aagctgccgt ttccagggct ttcattttct gatattgaag cgtatcttta
    18421 tcccgatact gatcgattac ttcctggatc aggactgcat cttccagctg tgcactgccc
    18481 tttttcagat gtcctttttc ttccagctct tttacactgt acacagagaa tccggcacct
    18541 ctcatccatt ccagatccgt ctctttattg tctccggtca gtttaaagca agtaaatggc
    18601 tgagatttga tgcgttcttt actgaacact tcttttttga tcacaagaat atcctgtcct
    18661 tcataggcac agtccaccgt ataaactgcg gcatcggcaa ggtatccttc cgaagctttg
    18721 atttctttta catagtacgt tccaagtggc agtcctgtaa aacaaagtct gccctctgta
    18781 tccgtctctt ttcgcatgac ttctgcattc ttctcataga gaatctctcc atcgccatct
    18841 gccgatgtaa tcgcttcttt tgcatagatg ccgtacacgg ctccttttaa agatgccatc
    18901 tgctgtggcc tggaaagtcc gctctgtgca tccactttct gaaccgtaac ttctgctgtt
    18961 acttcctggt tcagaaaaac ggcttctgat tcgcttccag gtttcgggaa tgtgtaaagc
    19021 tgtgttttat catcctgata aatccactga aactcataac gtgctttttt caccnnnnnn
    19081 nnnnctttta cataatagcg gtaaccgcat ggaacatccg catcaaaaag acagcttccg
    19141 tcttttttac tgtacgccag agcaagcagc gtatcttttg ctgcgatcct gtttccatcc
    19201 gacagaatgt tttctcccgc atacaatcca aaccgtgctc ctgcaagtcc cttctgtgtg
    19261 ttctcggagt gtttgaaaac cgtcacctgc atctttgggc gttcattttc aaagacctgc
    19321 tcttcgctta catattcccg gatttccggt ccgcggtagg aaagtgtcac tgttctggta
    19381 tttcctgctg ccgtatcttt tcgcacatag ccctgcggtg cctcggtttc tgtcacatga
    19441 tacctgccaa gatacaactt cctgctgact gccgtaccat cttttccggt taccagctgc
    19501 tgtgccactg cacccttttt cagagccagt gtcactccgt cggccttata gatatcttct
    19561 gccgctgtaa tctcgtattt tgctccggca agcggctgct tttcatagag aaatatcaca
    19621 ccctccgccg aatctctggc ttctgttaag acctcgccga tttttttcac cgcgatcgat
    19681 gccatctgcg gtgtattgac aaatcccgtc tgcttcccgt tgttggtcag ctccaggctc
    19741 tgtcctgcat tctgggcagt gatgcgaaaa tcatagaatt tcagcccttt ttctttctca
    19801 ccgtccacat aatctccaaa atagtttttc ggcggctgtg tttcctgaag tcgaaatttt
    19861 ccttcgttct ttttctgata gtaaagttta ccgctgactg cctctccgtt tatggctgtc
    19921 acaagctccg gccaagctgc atacggcaca taattttgct gttcctgatt ccattcgtat
    19981 accgcaaatc tcgcacccga cagtgctttt cctgtctcca gatccttttt ctgaatctgc
    20041 acggtgatcc acagcggttc attgaaacag gcgttgtcac cgtgatacgt gatttttttg
    20101 ctttcaaacg gtgagataac aaattccttc tcccatccac tctttgtgta gccttccggc
    20161 gactgttctt ctatgatctt gaattttccc ttgttctca
//
LOCUS       DAWDWH010000013        18054 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_1816_length_18054_cov_13.609867, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000013 DAWDWH010000000
VERSION     DAWDWH010000013.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 18054)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18054)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18054
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1816_length_18054_cov_13.609867"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    13953..13962
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17440..17449
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5167 a         3791 c         4668 g         4408 t
ORIGIN      
        1 acatggatgc tggaagacac ggcaaaacgg aatccgcttg acagatgtat ttctttgcgt
       61 gatacttagt tgaaaaacat ccgggggaag agagcggatg aacaggaagt ttaatcaaat
      121 caggtaatgt ccggcgttcg acttgaagtg cagggagttg cctgtatcag gaggatggca
      181 tgaaaatatt gctggtcgca atcaatgcga aatatataca ttccaatctt gcggtgtact
      241 gcctgaaaag ttatgcggca aagagaggaa ttgaggtgga acttacggaa tatacgatca
      301 atcaccgacg agacgatatt ctgatggata tttatcagag aaagccggat gtggtggcat
      361 tttcctgcta tatctggaat ttggaatttg ttcaggatat tgcagcagat ctgtacaagg
      421 ttctgccgga tctggaaatc tggggaggcg gaccggaggt ttcctatgat gcagagaaat
      481 ttctggcgaa gcatccggaa tttcgcggca ttatgaaagg cgagggtgag cggacttttt
      541 gtgaacttgc cggactttac cagatatatg gcaaagaggc ggtgaacaaa ctggacgagt
      601 gccatggtat tgtatggcga gacccgaaag gggagatcag tcagggactg ccgcaggcac
      661 caatcagtct ggatgaagtt ccttttatat atgaagaata tgaaaatatg atgccgtttg
      721 agaatcgtat tctctattat gagacgagca gaggctgccc gttttcgtgc agttattgcc
      781 tttcttcgat cgataagcga gtgcggcttc gaagcatgga gctggtagag aaagaattgc
      841 agtttttcct ggatcataaa gtgccgcagg tcaagtttgt ggaccggacg tttaactgca
      901 atcacagaca cagcatggca atctggcgtt acatcaaaga gcatgacaat ggcatcacaa
      961 acttccattt tgaaattgcg gcagatattt taaatgaaga agaaatggat ctgcttgcca
     1021 cattccggcc gggacttgcc cagctcgaga ttggtgtgca gtccacaaac atacagacca
     1081 tccatgagat cgaccgtgta atggattttg atatggttac acagaaagtg cagcgggtgg
     1141 caagtgccga aaatatccat cagcatctgg atctgatcgc cgggctgccg tatgagaatc
     1201 tggagagctt caaaaaatcc ttcaacgatg tctaccgcct gcggccgcag cagttccagc
     1261 ttggctttct gaaagtctta aaaggctcaa aaatgcatga gaaggcagga gaatacggga
     1321 tcgtttacca tacacgccct atgtacgagg ttttatcgac aaactggctg acttacgatg
     1381 aagtcatcta tttaaaaggc atagaagaga tggttgaggt ttactataac agctgtcagt
     1441 tccgctgtac aatgcgggca ctggaggcgg agtttaacac accgtttgcc atgtacgagg
     1501 cactggcaga atattatgaa gaaaacgctc tgaacggcct gaaacattca cgcatgggac
     1561 gctttgatat tttgcacgat tttattcttt cctatgtgaa gaaagaacat gcaccgaaat
     1621 atgaggatga tcttctgatg gatctttact tgagggaaaa gagcaagagc cgtccgtcct
     1681 gggctgcgga tctttcagga tataagagcg agatccagaa atttttcaga aaagaagcgg
     1741 aggaaaagcg ttatctgaaa gactacgaag gctacagctg gaaacagatc ctgaatatga
     1801 cgcacgttga ggtcgattcg aagggaaaat ggacgctgtt tgattataaa aggcgagatc
     1861 cgctcacaaa agatgcaaaa acgtatcgga ttctggaacg gaaggaagag gaaagggcat
     1921 gataagagat tatgtggcac ttgacctgga aatgacggga ctgagcccga gaaaggatgc
     1981 gatcctggag atcggtgcgg tgaaagtccg ggatatggaa gtgacggaga cctacactgc
     2041 catggtaaat ccagggtttc cggttccgga gcatgtcaca gagctgaccg ggatcacgac
     2101 cgaaatggca cagcagggca gggaaatcca cacggtattg ccggaatttc tggaattctg
     2161 tggacaatcc gtgatccttg ggcataacat aggctttgat tttggatttc ttcagcaaaa
     2221 tgcggcaaac atggggaaaa ccttcccgga tatggcgatc gatacccttg cgatcgcacg
     2281 caaatttctg ccggaactgc cgagccggaa gttaggtgat ctgtgtgatc attatcagat
     2341 ccgggaggag agatggcacc gtgcgtgcga cgatgcggat gcggcgagcc ggctgtatca
     2401 gaaactggca gaagcatttt ttgaggaaaa cgaaacttac tttgagccga aaaaacttac
     2461 ttataaggta agaaaagatg caccggcgac gaaaatacaa aaagtctact tgaatgattt
     2521 gatgaaatat cataaaatag aaacgaatgt tgccctggat ctgctgacga gaagtcaggt
     2581 ttccaggctt acagacagaa tcatattaca gtatgggaaa atgataaggg gtgattgata
     2641 tgatgcattt tcgtgacaaa aaaacaaaac ggattctttc aggtattctc gtggtactga
     2701 tcgtgatcgc gatggtactt cctacgctgg cagctgttat tggaatgttt taaagtatca
     2761 cacaggtgat atggaggtaa atgattatta tgaaaaaaat cggagtttta ggactgatgg
     2821 cagcactggc tgttacaggt gcagttgctt ttttgccgaa aggggttatg gctgccgaag
     2881 attctgatca gacgatcagc cagggcgtat atatcggaga cgtagatgtc tccgggatga
     2941 cgcaggaaga agcgaaaaaa gcagtggaag atggtatgag cgaagcgaaa gcggcgaaca
     3001 ttaccttaaa agtagatgat caggaaaccg tggtaagtgc gggagatctc ggactttcct
     3061 gggcaaatga agaggttgtg caggaggcgg cggagcttgg caaatccggc aacatcatca
     3121 aacgttacaa agacaaaaag gatctggaga atgaatccag aaaatatgac atagattatt
     3181 ctgtagacag aagcagcatc cagaaagttc tggaggaaaa agaagatgtc ttcaaccggg
     3241 aaccgaccga cgcacagctt tctacggaaa atggtgcttt tgaagtcgtt cagggcgaaa
     3301 atggaattga gctgaatgtg gagaaatcgg tcaaaaagat tttgaaatct ctcaaaactg
     3361 actgggacgg caaaaatact acgatcgagc tggcggccga tatcaaagag ccggagtgta
     3421 caacggagga tctggcgagc atcactgatg tactcggaac agccactaca tactacggat
     3481 cgaccacagg cagaaacaaa aatgtagaga caggtgcaga gaagttaaac ggacatatcc
     3541 tgatgccggg agaagagttc tccgtaacgg atgcagtcgt tccgtttact gaagagaacg
     3601 gctacgagct ggctccttcc tatgagagcg gaaaagtcgt agacagttac ggcggcggta
     3661 tctgccaggt ttccacaaca ctttacaatg cagttttaaa tgcagaactg gaagtgctgg
     3721 atcgccacaa tcataccatg atcgttactt atgtggatcc ttccaaagat gcggcgatcg
     3781 cagagggact gatggatctt cgttttgcga ataatacgga ctacccgatt tatatttccg
     3841 gctatgctta cggcggtgag ctgacgttca cgatctatgg ccatgagaca agagatccaa
     3901 atcgtacggt agaatacgtc agcgagacaa ccggaacaac gactgcggat ggtgtagcac
     3961 tctatgccac ggatcagccg gtcggctact taagccagac acagggagca ctgcaggggc
     4021 ttactgcggt tctgtggaaa tatgttaccg aaaatggtga gacgacaaaa gaacaggtga
     4081 actccagtac gtatcaggca acgccggtct gctatgatgt cggagtaaat acggataacc
     4141 cgactgtggc agcggcaata cagtctgcga tcgcaaacaa tgacctggat caggttcagg
     4201 cgatcataag cggaaattac ggacagacaa gtacagcgac agcggatcag gcacagagtg
     4261 agtcacaggc acaggatcag acacagagtg aatcacaggc acaggatcag cagcagagcg
     4321 aatcacaggc acagagcgat tccgcagcag acaatacgca gggtggagat ggaagttcca
     4381 caccggatgt ggcactgccg gatgacccga caaacggagc tgttattgag taaaaaattg
     4441 atagaaggat ataaactatg gatcttgttt tgacaaaatg gatagcatta aaaggcactt
     4501 ccgtccttgc ttcgtgcagg gcagaagagc tgtccagccg ttttccgtcc gtgatgatac
     4561 gtgatgcggc aggttttacc tgctatctgt ccacagagcg agagtaccag atatcaggag
     4621 ggtatggtgc tgccgccatt tacccgcttg gaaaaggcgg ggtattaggt gggctctgga
     4681 atatgctgga gcagataaat gcaggtatgg aaattgatct tcggaaaata ccgatccgcc
     4741 aggagactgt ggagatttgc gagttttttg acctgaatcc ctactatacc gattccaccg
     4801 gggcacttct tgtggcagtg gaagacggat ttgggctggt ttccgtgctg gaacgtgaag
     4861 ggattcacgc agccgtgatt ggaagaacga acgatggcaa tgaccgtatc atttataatc
     4921 agggaaaaaa gagatacctg gatcgtcctc agaaggagga actggaaaag gtgttcgatt
     4981 agaaggaggc ttaaaaatga gagaacagat attgacattt ttagaaaaaa acagccgtat
     5041 tgatccgaaa gaactggctg ttttacttgg cagcgatgaa gtaacgatca tgaatgagat
     5101 cgcaaagatg gaagaggaaa aggtcatctg cggttatcac acactgatcg actgggaaaa
     5161 ggtttccgat gagaaagtaa ccgctctgat cgaagtgcgt gtcactccga tgcgtggaca
     5221 gggatttgat tccatcgcag agcgtattta taattatccg gaggtaagaa gcgtatatct
     5281 gatctccggt gcctatgatc tgatggtcat tctggaagga aagactctga aacaggtatc
     5341 aggttttgtt tctgacaagc tttccacact ggattctgtt ttaagtacag ccactcattt
     5401 catcctgaaa aaatataaag atcacggaac gatttttaca aagaaaagca aagatgaaag
     5461 gatgctggtg acaccatgaa aaatgtaatt tccgataaag ttctggggct tcagccttcc
     5521 ggaatcagaa aattttttga tattgtaagc gagatggacg atgtcatttc gcttggtgtg
     5581 ggagaaccgg attttgatac gccgtggcat atccgtgatg agggtatcta cagtctggag
     5641 agaggaagga ctttctacac atccaatgca ggtttaaaag agctgcgtga ggagatctgc
     5701 agatatttaa gtcgcagatg caaagttgac tatacgatcg acgagacgct gatcaccgta
     5761 ggtggaagtg aggcgatcga ccttgcgatg cgtgtactgt taaatccggg cgatgaagtg
     5821 ctgatcccac agccaagttt tgtatcttat ctgccgtgtg ctgttttagc ggacggtgtt
     5881 ccggttgtca tcaacctgaa agcagagaat gagttccgtt taacggcaca ggagctggag
     5941 gatgcgatca ccgataagac aaaacttctg gtactgccgt tcccgaacaa tccgaccggt
     6001 gcggttatga caagagaaga tctcgaagcg atcgcagaag tgatcatccg ccacgatatt
     6061 tacgtgcttt ctgatgagat ttattctgag ctgacctacg caggagatca tgtatcgatc
     6121 gcaagtctgc cgggcatgaa agaacgcacg atcaccatga acggattttc taaggcttac
     6181 gcaatgacag gatggagact tggctacgcg tgcggaccaa gggagatcat caagcagatg
     6241 acaaaggtac atcagtttgc gattatgtgt gctccgacca acagccagta tgcagccgta
     6301 gaagcactga gaaacggtga tgatgatgtg atcaaaatgc gtgaatccta caatggcaga
     6361 agacgttatc tgctgcatgc atttgcagag atgggactgc cgtgctttga gccgcttgga
     6421 gctttctatg cattcccatg catcaaagag tttggcatga cttccgatga gtttgcatcc
     6481 agactgctga aagaggagaa ggtcgctgtc gttccgggaa gtgcatttgg tgagtgtggc
     6541 gaagggtacc tgcgaatttc ctatgcttat tcactggaag atcttaagaa agcactggac
     6601 cgcatggcag atttcgtgaa gagactgaaa gaagagggca gcagataatg gcaaagctga
     6661 atatcgtgct ctttgagccg gagattccgg caaacaccgg aaatataggc agaacctgtg
     6721 tggcaaccgg gacaagactg catctgatcg agccgcttgg cttccgctta aacgaaaagg
     6781 cattaaagag agccgggatg gattactgga aagatctcga tgtgactact tacattgatt
     6841 atgcggactt tctggagaag aatccgggag ccaagatcta tatggcgacg accaaggcaa
     6901 agaaaaccta cacggaagtg gcctatgagc cggattgtta cattatgttc ggaaaagaaa
     6961 gtgccgggat cccggaagaa attctggtag agaatcagga gacggcgatc cgtatcccga
     7021 tgatcaatga tatccgctcc ctgaatcttg gcaattctgt ggcaatcgtg ctttatgagg
     7081 cacttcgcca gaaccacttc gatcatatgc agctggaagg cgacctgcac cggctgcact
     7141 ggaagggata gctgcggagg ttttcttaat gtgcaaacga aatgcattga aaaacatagt
     7201 gcgtaaaata aaacagccat gatgaattcg aaaacaaatc gctctgacgg tagcgaggct
     7261 tccgtcgtga gattgttttc gaatcatcat ggcgtactat tttttacaaa actatttttg
     7321 caacgttcat ttgcacattt acaaaatctc cgtcattttt tgttcttgct gcatggcagt
     7381 gtaaatgtaa actcggttcc tacgccctgc gtgctgacaa cgttgatatt ttcaccgtgg
     7441 gcctggatga tctctttgac gatcgaaaga ccaagtccgg tgcctttttt atcttttcct
     7501 ctggatggat cgctcttata gaaacgctcc catattttat ttaagctgtc ctttgcgatg
     7561 ccttcgccgg tgtcttttac ggatacaaag acttttccgt gtttttccag agaagagatg
     7621 tagatcagag aatctggatg actgaatttt acggcgttgt cgatcagatt atagagaacc
     7681 tgctggatct ttccaacatc ggcattgacg tacagtgact cttcttcgaa cgtaagctgg
     7741 aaagaaatgt gtttgtccca gcacaggctg cggaaggttt ccacagtctg gcggatgatc
     7801 gcattgatgt cgaaatctgc aagttccagg tacgttccct tatcatcgaa gttgttgaga
     7861 gtcagcagtc cttttgtgag cttggtcaga cggtttgttt ctgagagaac gacattgagg
     7921 tatcgctcct gcatttccgg tgggatcgtg ccgtccagca tggcttctac gtaccctttg
     7981 atggaagtaa gcggagaacg gaaatcatgc gaaatgttgg aaataaattt tcgctggctt
     8041 tcgccggagt tgttcagctc gcctgccata taattcatag aagcggcaag gtaaccgatt
     8101 tcatcgttgg attcgaccgg gatcttgtag ttcaggttgc cggcggcata ttcatctgct
     8161 ccgtgtatga tgcggcggat cggaaaatat actacaaatg taaatgtaat caatacgatc
     8221 agagaaagag ccaggatcat agccagtgtg atgtaggaac agttgagaat ggaatttttc
     8281 tccgtttcta gtttgttctt ttcataatgg actgcaatgt agcctttcgt gatgtagttg
     8341 gatgtgatcg gtgcgaccac actgagcata tcttcatcaa aatagtcaaa aaagcgaccg
     8401 gtttgataat aattgccgga cagggatacc ggatcaaaag aatccagctg cgtgatcttt
     8461 gactgatcat agccgacagc cgtatcgatc aggatcttgc cattactgtc catcagccag
     8521 agccgcatgg actggtaaga ggcaacggcc agcaggttcg tgtaggtgtc ctgcaaagaa
     8581 gcccgctcgg tgtaattctg tgcgagcctt ccggcagcga tcacatttgc ttcgcggtac
     8641 atgccttctg tcctgttttt gatcagatgc ttttccacaa gcgaagaagt gataccagct
     8701 acggatacaa aactcagtac ggcgaaacag atccatgcca gaatgagttt ttggtaaagt
     8761 ctgcgtttca tatgttttta acctcaaatt tatagccgat accccatacg gtggtcagtg
     8821 cccaggaatc atttcctttg atcttttccc ggagacgttt gatatgtacg tcaaccgtac
     8881 gggtatcgcc gatgtattca tagccccaga tgtgatccag cagctgttct cttgtgaaaa
     8941 cctggtttgg tgaagaagcc aggaaataga gcagttccag ttcttttggc ggcatgtcaa
     9001 cggtagatcc tttatagata acagaatagt tggtctgatt tacgatcaga tccgggtatt
     9061 ccacgatctt gccggaattc tgtgaactgg aagatttctc tggctggaaa cggcggagaa
     9121 ccgctttgac tctggcaacc agctctttgg aatcaaacgg tttgatcatg taatcatctg
     9181 caccaagttc cagaccgagt accttgtcaa acacaccctt tgcagacaga atgatgatcg
     9241 gcacggaaga acgctgacgc agttcgcggc atacctgata gccgtccatg cccggaagca
     9301 tcagatccag cagaaccaaa ttcggtccgt aagtatcaaa ttcacgcatg gcactttcgc
     9361 cgtcgtgaac aattcgcgtg ttaaaacact ctttggtgag atacaaagag atcagctctg
     9421 caatgttctc atcatcatca acaatcataa tcttttgtct gtttaccata atatatactc
     9481 ctcatgtttt taaataatat ttaataatgc taaggtcaaa agcctgaaac cacgatgtgc
     9541 ttgcacagga gtggttttat ggcttaatga cctgcaccga tatgagcata aaagtggagc
     9601 gtaagcttca cgatatgcga atgtcggata gaattgtgag agtacgaagt acggaacaat
     9661 tcttaaggca ttattgattt taacagtttt atttgaactc cacgatcgta actccggcat
     9721 caccctcgcc gaattcgcca aggcgatagc tcttgacatg cttctggcgg cggagataat
     9781 tgtgaacacc ttttcgaagt gcaccggtac cttttccgtg tacgatacgg acggaaggaa
     9841 ggtgggcaat gtaagcatcg tcaagatatt tatccagctc catgatcgcc tcgtcaacgg
     9901 tctttccaag cagattgatc tctgtgctga cagatgccga tttggacatt ttgatcttgc
     9961 cggaaccggt cttgctgaaa ttgcctccgg tgatcacggt atcatccagt ttttccagat
    10021 cccggatgtt tacctgagaa cgcaggattc ccatctgaac aaacaggttg ccttttgcat
    10081 ccggcagcgt gctgaccgta cctttgaggt tcaggctcag tacctttatg ctgtcgccga
    10141 tgtgcagatc tttggcggta agctgtttat gcgatttttt cggagccgca ttcttcgcag
    10201 aaagcttttt gtcaactgtg gacattttct cacgcagttt gccgcgttcc ttttccatgt
    10261 ctttggcaga cacgccggca gcctttccat atttctggaa attgcggatc gtagtatcgg
    10321 cataatcttt ggcttcctgc aaaatgttgc gtgcctgctc atttgcttca cggagaatct
    10381 tgtcgcggct taagtccagt ttttcctgtt tggattcaag acgctgcttt aattcgcgga
    10441 tctcttcttt ataggaattg atctggtcac gttcctgttc tattgtaaca cgtttttgct
    10501 ccagatcagc gagtaaatct tcaaaatctt catcctgttt ggtcaaatgt gtttttgctt
    10561 cctcgataat gtcctgtgac agaccaagtt tctgagaaat cgcaaaagca ttacttttac
    10621 ccggaacacc gatcagcagc cggtaagtcg gacgcagagt ttccacatca aactcgcagc
    10681 agccgttttc cacgcccggt gtggaaagtg caaacacctt taattcgctg tagtgcgtgg
    10741 ttgccataac gcgggaaccg cggcgatgca ggttggaaag gatcgcgatc gcaagggctg
    10801 caccttcggt cggatcggtt ccggcaccaa gctcatcgaa caacacgagc gaacggtcat
    10861 ccgcttcttt taagatcgat accgtatttg tcatatgggc ggaaaaagta ctgagactct
    10921 gctcaatgct ctgctcatcg ccgatatccg caaaaacttc gtgaaatacg gaaagctctg
    10981 agtggtcaaa tgccgggata tgcagtccgg cctgtcccat cagggtaaaa agtccgactg
    11041 ttttcaggga aacggtttta ccgccggtat tcggaccggt gatgatcagc agttcaaaat
    11101 ccttaccgag ctgaatgtcg atcggaacga ctttcttttt atcgagaagc ggatgtcttc
    11161 cttttttgat gaggatacgg ccttcctcgt tgaaatccgg ctgtgtgccg ttcatcgagc
    11221 gggagagctg tgctctggca aaaatgaaat ccagttttgt cagaagatcc aggtcatcac
    11281 gcagagcttc ggtttcttca ccacatctgg cacttaaggt ggcaaggatc atctcgattt
    11341 ctttttcttc ttttaattcc agttcacgca ggtcattgtt cagtttgatg acagccatcg
    11401 gctccacaaa cagggtagaa ccggtggagg actgatcatg gatcatgccc ggaatctgtc
    11461 cgcggtgctc tgctttgacc gggatacagt agcgtccgtt tcgcatggtg acaaccgcat
    11521 cctgcaagta ggtgcgggcg gagccattta ccatcgtctg gagctgagca tggaccttgt
    11581 cgttgatctg acgcatggag cggcggatct tgaacagtgc aggactggca tcgtcattta
    11641 attcctcctc cgaggcgata catctgcgga tctcggaaga aaccggtgtc agaggctgca
    11701 gtgcctgaaa catagcttcc agagaatcct caaaatcagg atcacggtca tttctggaat
    11761 aagcctttac cttgctgcag acatcaagaa gactgcagat cgaaagcagt tcattgatac
    11821 cgatgatcgc accgatctcc agacgcttga tggagccgcg gatgtcgcgg acgccgcgga
    11881 acgataccgc acctttctga taaatacggg aaagggcatc gcttgtctcc ttctggagag
    11941 cctgaatctc gtacagatcc gtggatggct gcagattgcg gcacatctct tttgcaagtg
    12001 ccgaaccggc aaattctgtg agcttgtcaa tgattttatt atattctaaa gtttttaaag
    12061 cctttgggtt catatagcat acctcttgta gtgatgtgta acaaaagatg ccatacgaat
    12121 tgttccgtac ttcgtactcc cacaattctg tccgccattc gcatatcgtg aagcttccgc
    12181 ttcactttta tgctcacgtc gggcggggca ttaagccata aaagcactcc tgtgcaagca
    12241 catcgtagtt tcaggctttt gcccctggca tcatataatt ataactttac tatttttcta
    12301 ttgtacccta taatgtgggt atggtcaaaa ggtattttga gcgaaaagcc tataatagca
    12361 gaagggatca ggtatatggg tgagaacgag aagaaagagt ttcattttat caatgaacag
    12421 atcaagaaaa agccgttcta ccgacgtaag tggtttacgc agggggcagc ggcgattgcc
    12481 ctggcactta ttttcggggt ggcggcgggc gtgatgttcg cgatcgtaca gccgtgggcg
    12541 agcaatcagt ttggaaagcc ggatgagccg acacagatcg tgatggttca ggaagagaca
    12601 catactcagg agacagaaac cggacagaca gagcagaaag cggatgacga aaaggagacg
    12661 gcaaaaggca ggaaaaagaa cagcaaagaa acagatgcaa tcgaagaata ccgcatgcac
    12721 tatgatcaga tgaaaaatgt ggtagattct gcagaaaatt ctctggtaaa gattaaaagc
    12781 tatgtggcaa aaatggactg gttcagtgaa agctatgaga atgtgacgga gacttccgga
    12841 ctggtgttcc gcgtggacag caactggctt tacatcctga cttccagtcg ttttatcaag
    12901 agtgcacagc agatcacagt aaccttcccg ggcggagagg tcgcagatgc agctgtcaga
    12961 cggcaggata cggtgacaga gcttgcgatt ctggagattc cgatcaaaag tataaaggaa
    13021 tccacaatcc agagcatttc cgcaatttcg atcaaaggaa tttccagcgt agaaaaaggg
    13081 gaatcggtga tcgcagtcgg aagtccaatg gggtatacag attcgattaa ttatggcatg
    13141 atcacttcga ttacggagtg tgaagatgtg gacggagagt ataaggtgat cgcaacggac
    13201 atggcggcca gcgatatgag ctatggattt ctgctgaatc tggatggaaa tatggtcggc
    13261 attgtggcac agaaattcaa acagactggt gcggcagata cgttgactgc acttggcatt
    13321 tctgatatca gctatctgct ggaaatgctg gcagcaggca ggtcgcttcc ttataccggt
    13381 gtggtcggaa agagcgtcag tgccgatgtg gcagagcatt tcagcgttcc ttacggtatt
    13441 tatgtcaaga aagtgaacac agattcaccg gccatgtatg ccgggataca ggcagccgat
    13501 gtgatcacag agatcaacgg ggaaagcgtt ggctcgatgt ccgagtacga agatattctg
    13561 cggaaatatc aggaaggtga taccttaaaa atcaaggtcc gcagaaaaag tatcggcggt
    13621 tatgccgatg tagaaatgaa actggtcatg ggagccagat aacgaaacag ataagatcat
    13681 ggtacctgca gcaagtgatc tgtcctgcac ggaaccattc atgtttattt aataagagaa
    13741 aggtcaaaac aatgaaatat attgaaacat tgcacgaagg cgagagactt tcaggaattt
    13801 atctttgcaa atacagacaa tctgcgatga caaaaaacgg caaatcatat gagaatgtga
    13861 tccttcagga caaaacagga gtgatcgatg ccaaaatctg ggatccgaac tctcagggaa
    13921 ttgaggattt cgatatgctg gactatatcg atnnnnnnnn nntggagatg tcacaagttt
    13981 tcagggagca ttacaggttt ccatcaaaag agtacgaaaa gcaagagaag gcgaatatat
    14041 cgaagaagat tatctgccgg tcagcgagcg aaacattgat gagatgtacg aagagctgct
    14101 cacgtatgtg cataaaatcg agaatccgta tctgaagcag cttacagaca gctattttgt
    14161 ggaaaatgaa gcttttatca aagcgttcaa aggacattct gcggcaaaga gcgtgcatca
    14221 cggatttgtg ggcggacttc tggagcatac cttaaatgtg acaaaactct gctattacta
    14281 tgtaaaacag tatccgtttt taaataaaga ccttctgttg tgtgcggcac tgttccacga
    14341 tgtgggaaaa atgaaagaaa tctccaaatt cccggaaaac gattacacgg atgacggaca
    14401 gcttctcggc catatcgtga tgggcgtgga actggtcgga gcaagtatcc gccgcattcc
    14461 ggattttccg aaaaaactgg caagtgaact gaaacactgc attctggcac atcacggaga
    14521 actggaatac ggttccccga aaaagccggc actggcagaa gcactggcac tgaattttgc
    14581 agacaatacc gatgcaaaac tcgagacatt taaagaagca ttaaagagca ataacgataa
    14641 caatgactgg cttggttata accgcctgtt tgagtcaaat atccgcaaga caagtatctg
    14701 atcagacaat gtgaaacagt taaagaagcc ggaaaagttt ctgttcatgg aggaattaaa
    14761 tttatgaaaa aagacgatat gctggaaatt accatagagg atatcagctc agacggttcc
    14821 ggcgtgggaa aagcagatgg atttgcactt tttgtcaaag acacgatccc gggcgatcag
    14881 gtgaaagtca aaatcatgaa aatgaaaaaa cgttatggct atgcaaggct gatggaaatt
    14941 cttgtgccgt caccggaccg catccaggca ccgtgtccgg ttgccagaca gtgcggcgga
    15001 tgtcagatcc agcagatgtc ttatgaacgt cagcttacct ttaaagaaaa caaagtctgc
    15061 aacaatttaa gacgcctcgg cggtctggat ctttctgcga tggaagtaga gccgatctgc
    15121 ggcatggaag agccatttca ctaccgcaac aaagcacagt ttccgatcgg ccgcgataaa
    15181 aacggaaaaa tcattgccgg attttatgca ggccgcacgc acagcatcat agcaaacacc
    15241 gactgtctgc tcggagcaga agaaaatgaa gcaatcttaa acgaagtcat ttcgtggatg
    15301 gaagtctgta aagtagagcc atatgatgaa aagaccggaa aagggctggt gcgtcacgta
    15361 ctgatccgca agggatttac cacaaaagaa ctgatggtct gcctcgtgat caatggcaga
    15421 aagattccac aggttgaaaa attggtggac aggcttcaga agatcgaggg catgaccgca
    15481 atcatggcaa atgtcaacac tgagcagaca aatgtgatcc tcggaaaaga aatccagccg
    15541 atctggggtc aggattatat cgaagattat atcggaaacg tcaaataccg catttccccg
    15601 ctgtctttct atcaggtcaa cccggtacag accgaaaagc tttacggaaa agcactggaa
    15661 tacgcaggac tgaccggtaa agagaccgta tgggatctct actgcggcat cggaaccatc
    15721 tccctgttcc ttgcacagaa agcaaaacag gtttacggcg tagagattgt gccgcctgcc
    15781 atcgcagatg cgaaagacaa tgccagaaga aacggcatcc aaaatgcaga gttttatgtt
    15841 ggcaaagcag aagaaatcct gcctcacaaa tacgaaaccg aaaacgtcaa agccgatgtg
    15901 atcgtagtcg atccgccaag aaaaggctgc gatgaagccc tgcttgagac catcgtcaaa
    15961 atgcagccgg aaagagtcgt ctacgtcagc tgcgattccg cgacactggc aagagatctg
    16021 aagtatctga ccgcgaacgg atacaaagca gaacgcctgg caacggtcga tatgttcccg
    16081 atgacggtgc acgtagaaag tgcagtaaaa cttgtgaggg aaaaccaacg aatagaaaca
    16141 agggtgaaat aacaatccgg aatttccgga tagttcaaac cgaaggttct cgtcaggtca
    16201 cacgcgatac caaggactac accatcaaag gctgggtgat agatgatgaa cgactgaaac
    16261 gaggaacgta tctgacagag aagtatttcg atgagcagtt ggagtgcatc cgtgaaatcc
    16321 gtgccagcga aagaaagcat cacagccatt tgcggcattt tgcagcaaat gcagggaact
    16381 tggatatgag atttatgata tggatctgat cacggcaggg gaagaggctt atgcagccat
    16441 gcggcgaagc acaaacgggg gcggcgagaa ttctccgatg ctggaagggg aagatgacct
    16501 ttacgagctg ttttttgcag gattgtaggc gactgtaaga agaaccatat aaaaaagacc
    16561 tcatacaatt cttttgaata aggattgcat gagatctttt tttatgaaat tattttttcg
    16621 caaaatattc ttttgtcaga gcgatggcag ttccggaaag aaggaagata ccgatcaggt
    16681 tcgggattgc cataagtccg ttgaaggtct cggcgatgct ccagagaagt ccgagatcga
    16741 ccgttgctcc gatgatagct accagtgaat aggcgatcat aaacggtttt actactttct
    16801 cggaaaacag gaactcaata cagcgtgcac cgtaaagtcc ccagccgatg atcgtggaga
    16861 aagcaaaaca gcacatggca actgcggtaa aaacagatat ccagtttccg taggtagaag
    16921 tgaatccgga gattgtcagc tcggcacctg cggcagctcc gtagttgacc ggaacaccgc
    16981 ttaccagaat gacaagggca gtcagagtac agataacgat cgtgtcggca aatacttcga
    17041 aaataccgaa cataccctgt tttaccggtt tgctggtatc agcacaggca tgggcgatag
    17101 aaccggtacc aagacctgct tcattggaaa agataccgcg ggatacacct tttttcaggc
    17161 tgaggaagaa acttccgatc acaccgccgg tgacggcagc cgggttaaaa gctccgtaaa
    17221 cgatatcatg gaatacagca ggaactctct caaaattcag tacaaccaca ccgatcgcaa
    17281 gaacgatgta caacagtgcc atgaacggaa ccagtttttc ggagacactt ccgattcgtt
    17341 tgataccgcc aagcagaacc ataccgacaa gaagcatgat cacaatacca agaaccagat
    17401 tcagcgtttt ggatgcagat tccggaatga tgttgaaatn nnnnnnnnnt tgaaattgat
    17461 cagagcagtg tcgatcgcag tagtgattgt atttacctgt gtggcatttc cggtaccgaa
    17521 aacggtcagt acaccgaagg cggaataaag aattgcaagg atctgccatt tcttgctcag
    17581 tccgtttttg atgtagtaca tcggaccgcc gacataatca ccctttttat ttcgttcgcg
    17641 gaaatgaacg gcaagtgtga cctcagtgaa tttcgtgcac attccaagga ttgcggagat
    17701 ccacatccag aacacggcac ccggaccgcc gatcgcgatc gcaccggcga caccggcgat
    17761 atttccggta ccgaccgttc cggcgagggc cgtacagaca gcctggaaag gagtcatgga
    17821 gccgtcagaa gcttccttct ttttgaaaat tcttccgagc gtggttttca gtgcataagg
    17881 gaatttgcga atctggataa atccggtccg caggcttaga taaagcccga caccgataat
    17941 gcagatcatc gccgggacac cccagatgaa gttattaacg gcattgttga tggattcgat
    18001 tgtctgaagc atgatagtaa tcctttctta agataatgta taattataaa tcta
//
LOCUS       DAWDWH010000014        18051 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_1817_length_18051_cov_13.501278, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000014 DAWDWH010000000
VERSION     DAWDWH010000014.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 18051)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18051)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18051
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1817_length_18051_cov_13.501278"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    440..449
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2135..2144
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9393..9402
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13602..13611
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14912..14936
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15325..15334
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4641 a         4516 c         3736 g         5083 t
ORIGIN      
        1 gtccgtagtt gttgctgcag tttgtgatat ttgccgggaa tttgtaagta tccatgtagg
       61 cttttaccag catatccgaa gatgccttgc ttgcagaata cgggctgtga ggatcataag
      121 gtgtcgtctc gtagaaatat tccccctcgt tctccagaga accgtaaact tcatccgtgg
      181 atacatgtaa aaatttcttg tctgccttga atgtgccgtc ctcaagctcc caggctgcct
      241 ttgccgcatt gagcatgacc agagtaccaa gcacgtttgt ctttacaaaa acttccgggt
      301 tgcggatgct tcggtctaca tggctctctg ctgcgaaatg aaccacacgg tcaatgtcat
      361 tttcatcgaa gatcttattg atcgcctcgc tgtcgcagat atctgctctt acaaatgtat
      421 aattgtcacg gttctccacn nnnnnnnnnt ctcttaaatt ttccagattg cctgcgtaag
      481 tcagcttgtc aacgttgata atacggatgt catccccgta ttttttaaac atatagtgaa
      541 tataattaga accaataaaa ccggcacctc cggttacaag ataagttctc atgttctttt
      601 ttacctccat cagtcagttc gtttctctta ttttccatac ggattcttta tattatacca
      661 tctggggcaa caatattcaa ccggaaaact ccttttggca aagcttccaa taaagccttg
      721 ctttttcctt tgcttttcag acattctatg atataatata gccactgtta tcgtaaaaaa
      781 gacttgagag gagcattatt tatgaaagaa acaacacttg ttatcctggc agcaggcatc
      841 ggaagccgtt ttggcggcgg catcaaacag ctggcaccgg tcggaccttc cggtgagatc
      901 atcatggact attctatcta tgatgcctta aaggcaggat tcaataaaat cgtatttatc
      961 atccgcaagg atatcgaaga ggacttcaga cgcatcatcg gggatcgcat ctctgaaatt
     1021 gcagacgtat cttatgcctt tcaggatctg tccgctctgc cggacggttt ttccgttccg
     1081 gacggccgca ctaaaccatg gggcaccggc caggctcttc tgtgctgcaa gggcgtgatc
     1141 gacacaccgt ttgctgtcat caatgcagac gattactatg gaaaagaagg ttttaaaaaa
     1201 gtgcatgatt tcctggtaga ggagcacagc caggatgacc tgctgcattt ttgcatggca
     1261 ggctttatcc tcggaaacac cttaagtgag aacggcggcg tcacacgcgg catctgtcag
     1321 gtagatgaca gagaactttt aacttccatc gttgagaccc acgatatcac caaaacagca
     1381 gacggtgccg gtgttccgga tggaaacggc ggttttgcac cacttgatac aaacagtcat
     1441 gtttccatga atatgtgggg atttacaccg gaaatcctcg attatctgga tcaggatttc
     1501 gcagcattcc tttctgccca gaaagaaggc gatctcaaat gcgaatattt gatcccgaac
     1561 gttgtcgatg acatgctgaa aaaacaactt gctgatgtgc aggtactgga gacacgtgac
     1621 cgctggttcg gtgtcactta cgccgaggac aaggcttatg tcacagactc catccaggca
     1681 ctcgtaaaag gagggatcta ccctgaaaaa ctcttccaat aaacgggctt ctcttttcct
     1741 gcatggattt ctgctgaccc ttggcatcac atttgccccg gtcagcgccc acgccgggga
     1801 agctctccta cacccacaac ttccggctgt tccggtttat gaatttctct ccgatacttc
     1861 agtggaaggt gatctgcaga cagatactct ggaaaacaac gcaaaaagag cgacggtact
     1921 gccctccaga tacaatgcgg cagagcacga tctggtgaca ccggtcaaag atcagaatcc
     1981 ctacggaacc tgctgggcat tctccgccct ttccgcctgc gagacgagca tgatcagaaa
     2041 aggactgcaa gataccagct ttgatctgtc cgaattccat ttcgcctact ttttctttca
     2101 cactcaggaa gacgctcttg gtggtaccgc cggtnnnnnn nnnnttccag cctggcttac
     2161 caggatgtcg gccaccttca gaatgtccgc tatgtaaacg gttctgacac tgccagtatc
     2221 aaacgtctga tcctgcagta cggttcggta tccgccccgc tctgtgtaaa cctgaaaaaa
     2281 tattattcaa agtctaccgg tgcttattac tgcaacaaca acaccggaac aaaccatcag
     2341 ctcacgatcg tcggctggga tgatgattat tcgacggcaa atttcacaag tggcatacgt
     2401 ccgagcaaaa aaggtgcctg gattgcaaaa aacagctacg gcgaagattt cggcaatgat
     2461 ggctatattt atatttccta tcaggacaac agcctgaacc accagaagag atcaaccgct
     2521 gattcggatt ctctggtatt cgcctatgat atggaaaaca gcgataatta ttcccataat
     2581 tatcaatatg acggttccgc ttcctgtaca tacatgccaa tcccatccgg aagttccctt
     2641 tccaatattt ttactgtaag tggaaatccg aacggacagg aaaaattgaa atccgtttcc
     2701 tttgccctgg catccgaaaa tatccagtat gcgatccaga tctacaaaaa tccaacagcc
     2761 ggtgacccaa caagcggcat cgcagtcctc gaccgtgcac agagcggcgt taccacttac
     2821 tgcggttatt atacgattcc tttaaataca gagatcgtct tcaatcaggg tgaccgattt
     2881 tccgttgtga tctcctttgc atcgccgacc aaccagacac tgtatgcctt tatcgacaaa
     2941 agcagctctc tggaatccct gcatttcgtt tccagcgctg agattggcca gagctactac
     3001 cgcacaaaag ccaatggctg gatggatatc agttatcagc agatcaataa ccgcatcaaa
     3061 gcatttacgg aaaatacaac ggaagctgcc accgagattc tggcaaatgt ctctgctctt
     3121 ccaaaatcct cgatccgcaa gataaaaagc agtcgctgct cttctttacg gctttcctgg
     3181 aatgcggtac agtatgcaga cggctatcag attttccgca gcacttccag aaagaaaggc
     3241 tacacactga tcgctgacac gaaaaagact tcttattccg acaatactgt cgttcccggc
     3301 aggaaatact actaccggat acgtgcctat gccagaagcc gctacaacga tattgtatac
     3361 agtccttatt cccaggtgaa aaatcagaca ctgaagccgg aaaaagcaca gatacgctcc
     3421 ctgaaaaaga agagcagcaa aacctacctt ctgagctgga gaaaagtccc cggtgctgat
     3481 ggctatgaag ttttccggca gatttccggc aaaaaatggg aactgttcaa acgtatcaaa
     3541 cgaaccggca ccctgaaact gaaactttct ctcgcttcaa aaaaagactg cttctaccgg
     3601 gttcgtgcct ataaaaaaac agctaaggaa aacatttacg gttctttctc aaaaaaagta
     3661 aaaatttctg caaaataaag ccgaaaacaa aagcctccgc ccatcctatg atgagcggag
     3721 gctttttcta tcttatcctt ttcacacact cctacagaat ggatttccca ggtccatcct
     3781 gcatctctgt aattatctct aagtcaccaa ccaccagttc aactggtggt ttgattttag
     3841 ccctccaaag ggctatatta ctactcgccc cacagggcgg tgtcacaagc acttttactg
     3901 gtccgctata aagcggtact actagaatct tcttttcttg attcttctgc ctgctctttt
     3961 atatacttct gtacagcttc atcagtgata ttaccaatgg tttcgacata ataccctcgc
     4021 gcccaaaatg ctttgttcca tttactttgc aattctgggt gcctatcata aatcattagt
     4081 gtactctttc cctttaaata tcccatgaaa cttgaaatac tcagtttagg cggtattgct
     4141 acactaagat gtacatgatc cacgcaaact gcacctgcaa taatttctac attcttgtag
     4201 ttgcacaatg tttttattat ttctctaaca tcttccctca cttttccata caacgttttt
     4261 ttcctatatt tcggaatgaa aactatatgg tactggcatt tccagcgggt atgtgatatg
     4321 cttttattgt ccacttggac cgcctcctat tctttgagtt ggcttgcgac accatactca
     4381 ttgtatcata ggaggctttt tcttgatatc ctcaccgttt ccacttacac cattctcccc
     4441 agcatagctg ggggtttagt actttcaatt acaatactta ttaaaacact tgctttaaat
     4501 gatactcgtc atctaccagc agcatatcgg cctcttttcc gacactgata gaaccgactc
     4561 tgtcatagac acctatgctc tttgccggat ttcttgttgc tgcaaaaatc gcatctgctt
     4621 ccggaatatc gaaagacatt gcaatcttca tgcagtcgta caggcaggtt gcggatcctg
     4681 cgatggttcc gtctgccagt gttgctttac ggtctttcat aaatactttc tgaccgccca
     4741 gttcatattc tccattttcc ataccgcatg ccatcatgga atcactgatc agaacgatac
     4801 gttccggtcc gaacatacgg aatgctgcac ggatcgcact gccatgaatg tgcagaccat
     4861 cactgatcag ctcggcatat acatgatcgt tgtctgcagc tgctccgata acacccggtg
     4921 ctctgtgatt taatggcggc attgcattgt acagatgtgt tacatgatct gcaccggcct
     4981 caaatgctgc ctttgcagta tcataatctg cggttgtatg tccaacagaa atatgcactt
     5041 catctttgag ttccttaata aattctacag aaccttccat ctccggtgcc agagtcacca
     5101 gtttgatcag tccgccgctt gcctgctggc atgcacgaaa cgtctctgca gacggtgcct
     5161 gaatataagc accattctgt gctccctttt tctccggtgc gataaatggt ccttccatat
     5221 tgataccaat gacacgtgct ccgtctgcga tgccgtctgc ttcttttgcg gtcgcaaaaa
     5281 tatcaaccag catctctttg gaaagtgtca tggatgtcgg gcagtaagaa gtgataccat
     5341 gtgctttttc aaaacgtaaa atctctttta cgccttctac gtcgcagtca cagaaatcat
     5401 gaccgcatgc accatggctg tgtacatcca caagaccagg aatcagtttg tatccgtttg
     5461 cgtctaccac cgtcttatcg cttacttctg cttccgttgc cacgatctta tgatcttcta
     5521 caaaaacatc ttttacctga aatgttccgt cttcacaaaa aacggatgca cctttaatga
     5581 tcatttagat cactcccttc tctctgcaca gggacagtgc ttcttcatct gcaattacga
     5641 tcacatcgtt gtgaagctgc agtacagaag ccggtacgcc aggtacgatc ggcccgaaga
     5701 aagattcata aactgccttt gctttgttct ttccgcttgc cagcagcaga actttcttgg
     5761 ctgacatgat gttcttgata cccatggtgt atgcatattt cggcacatca tccatagttt
     5821 caaaaaatct tgcgtttgct ttgatggtat cttctttcag tgctacacag tgtgtcaggc
     5881 tctcgaaagc tgaacccggc tcgttaaatc cgatgtgtcc gtttccgccg ataccaagca
     5941 gctgcaggtc gatgccgcca acgctgtcga tgatcgcatt atattttgca catgcaactt
     6001 tgctgtccgg ttctgttcca tccggtacaa aggtacgtgc cttgtcgata tttacatgat
     6061 caaacagatt tttgttcatg aaataacggt agctctgatc attgtctccg gaaagtcctt
     6121 tgtattcgtc caggtttaca gaagttacct gtgaaaaatc gatgtcacct ttgttgtacc
     6181 attcaaccag ctgttcgtat gcacctaccg gtgtagaacc tgttgccaga cctaaaacag
     6241 cgttcggttt catgataacc tgggcagaaa taatatttgc tgcctttctg ctcatgtcct
     6301 tataatcttt tgcctgatat aaaaccatat ccctcttcct ttctgatttc aggtttattc
     6361 cacttccgct tttgcaaaaa agaccggaag atggaagttt ggcttctcac tgtcaattgg
     6421 tgaaaagctg atataatgtt caacagccgg atcttctgaa attttataga aattacagta
     6481 tatctccttg ccgctgccat ccggtaaata atcgcagact tccttcagaa acttctccgg
     6541 aatgagcagg ctcattgtcc agtgatcctt ttcaatcacg gaatttacgc ctgtttcctt
     6601 ataaacttca tcggaaagga actgtcttcc ctgtctgcca aagccatatt ttgcatacat
     6661 ggctccgttt gcattcactt caaagttcag atacatatca ttgttggtaa gttcctttcc
     6721 tttttctgcg aatgccagaa acacttccac tgcactgtct ttgcagacca tttcgtgatg
     6781 ctctgtgcat cttgcaagcg gatcatgctc ctctgtgcaa atctgcacat agagtccttc
     6841 ccccttcaga taccccatat aaccacaggc tttcggaggt gtgatcgcat gatgccactg
     6901 cacatggtcg atctcaaaac gattgcagtt cacaatctct tctttcgaag aaatcgtctt
     6961 aatcttatat tccaatgtat gtcctccttc taacggcacg agtaccaaaa gggggacacc
     7021 cactacggcg tcccccttgc aggtcagatt tcagaccatt atatcaagct attatttgca
     7081 cagtttcttg aactcgtcag ctacgaactg aacctgagtt ccgatgataa cctgtacact
     7141 tgttttgcct gggcgaatca caccagctgc tccagctgct ttgattgctt tttcgtttac
     7201 ttttgtagta tctttcactt ccagacgaag tcttgtgatg cagttgtcga tagaagtaat
     7261 gttttcttta ccgccgcatc cagccagaac tgcttttgca acttctgtgt agttgttgtt
     7321 agacagtttg atgctctttt cagattcgtc gatatcatca tcttcacgtc ccggtgtttt
     7381 caggtcgaat tttgtgattg cgaaacggaa tactacgtag aatactacaa atgctgcaat
     7441 acccagaggg atgatcagcc atgtcttagc tgctgccgga agggaagcag agaagaccag
     7501 gtcagttaaa ccagcggaga aactgaatcc ggctctgaat ccaagtacaa ctgtgattac
     7561 tgtgaagata gcgtacagca gagcgtatac tacatacagt ccaggagcca ggaacatgaa
     7621 accaaattcg aacggctcag ttacaccaca aacaaatgct gcgattgctg cggaagaaag
     7681 aagaccgatt gcaacttttt tcttgtttga ttttgctgta tgaaccatag ccagagcagc
     7741 acccgggata ccaaacatca tacatgggaa gaatcctgac atgtacatac caaggctcca
     7801 tgaaacatct gcagatgttt caccagccca gaagtgagac aggtcaccaa gaccgatggt
     7861 atcaaaccag aatacgttgt tcagtgcgtg atgcagaccg aacgggatca gcagacggtt
     7921 gaagaatgcg tagataccag cacctacagc tcccatcttt gcgatacctt ctcccagtgc
     7981 aaccagaacg ccaaacagta ccggccatac gaacagcaga actacggata caacgataga
     8041 aacaacacct gctacgatag ctacgcaacg tttgccgctg aagaatgaca gccaatccgg
     8101 cagctttgtt cctttgaatt tattgtagca ggaagaaccg atcacaccgg cgatgatacc
     8161 gatgaacggg ttcgcaattt tgctgaatgc aatgtaagca gttgtacctt cttcaagagt
     8221 tttgataaca gatacagcac ctacagacag cagagttgtc agcatcagcc aagaagccag
     8281 agctgcaagt ccacctgtac cgtcgttgtc ttcggacata ccaacaccga taccgattgc
     8341 aaacaggata gccatgttat cgatcagggc tccacctgct ttaactaaat agaaaccaat
     8401 ctgctgtaca atacctacta cgtcaccacc ctgcatggta gatgggcaaa gagcatatcc
     8461 aagacccatc aggataccac agataggaag gcaagcaaca ggtaacatca aagctttacc
     8521 taatttctgt aagaatttca tcataattac ttttttcctc cttctcttac ttaatgagaa
     8581 tcctcctcca gctctttttc cggctggatt ctgcggatat gtacggccag ataagccgtc
     8641 tcctcatctg taatttcgtg atcatagcgc atttttatat aatgcgcaat cttctcgctg
     8701 cacgcatatt caaccgggta cattccggcg atcacttttc cgaaaccgtt atcatcatcc
     8761 ccgagcatct gccccgtaaa aatgcgctgt ccaagaaatt tcagatgagt gatgaatctg
     8821 gcgtaatgaa ggctttcttc ctgcagttca atattcagat attcactgac gatattgacc
     8881 acgtcgcgaa tgagacaggt gatgttcatc gtattgcgta caccggtgtc atattccgca
     8941 tttacgatat gaagtgcaac ggatgcggcc tcatcctcat tcaactccac atcaagcttt
     9001 tccctgacct tgcggattgc atactttcca acgccaaact cgatcggata aaaggtcttc
     9061 acctcaacct tgagcgggtt gtcaaactgc attcctttct tataccgttc aattgcgaaa
     9121 ctgatatgat ctgtcagagt cagataaata ctctggtgta acttcagccc gatattgttc
     9181 ttggcataag tgataatctg gtcggataac ttgagatatt ccaccggaag cctctccacc
     9241 agcctggcga actgcccggc tgacttctta tcctccagtc ggaacgtctt ctcaatctgc
     9301 tcctcattca caatctgtcc cggctttgca tggaaaccga taccttttcc catgatgacg
     9361 acttcctcgg cgtctttgtc caaagcactt acnnnnnnnn nnacttacta cattgttatt
     9421 aataacttta atgattttca tagtaataac gtgtctccca aacaaaaaga cccaaagaat
     9481 ccagaatcct tctcctctga aactttgggt cttgcctgca tcaccagtaa caatcctgtc
     9541 ataactacca aattctttaa ttgcatctct actatagcag taaatttgaa caatgtcaat
     9601 gcttttttaa tcttttgttt atggattgtt tataattttt atttttctaa aaatgttttc
     9661 tgtaatgcag tgattcctct agtttccaat acatttcctg actgcttttc cttttcccgt
     9721 tcctatgatc acatgatgcc tgcgaattgc tccgttgcaa agtaactccc gcaatctatg
     9781 ctccgcttcg ttctgcacga aacgagtgcc tgtaaacatc cggaatccgc atatttactg
     9841 cataaacagc tacttcctca tggattcaag aatctctctt gccacgtaaa ctccgcttgc
     9901 ggatgcatgg gaaagggaat gtgtcacccc actgccgtct ccgatgatat agagaccttt
     9961 gtggatactt tcaagatgct catcaagctg tacttccata ttataaaatt tcacttccac
    10021 accatagagc agcgtgtcat cgtttgccgt tcccggtgcg atcttatcca gtgcatagat
    10081 catctcaatg atgccatcca ggatacgctt cggcagcacc agacttaaat ctcccggtgt
    10141 tgcggaaagt gtcggtctta caatgccctc ttccactctc ttgacggtac ttcttcttcc
    10201 gcgtaccaga tcaccgaacc gctggaccat aacaccgccg ccgagcatat tggaaagtct
    10261 ggcaatgctc tcgccgtatc cgttgctgtc cttgaacggc tcggaaaaat gcttggatac
    10321 aagcagtgcg aagttggtat tttcggtctg tttggtcgga tcttcgtagc tgtgtccgtt
    10381 tacggtcacg atgccgtttg tattttcact taccacgatg ccatatggat tcatgcagaa
    10441 cgtacgcaca ttgtcctcga actgttttgt acggtaaaca atcttgctct cgtaaagctc
    10501 atcggtcaga tgggaaaaga tcacagccgg aagctccaca cggacaccta agtccacacg
    10561 gttggaactg gtcgggatgc tcatctcttc acagaccttt tccatccatt tgcttccgct
    10621 tcgtccgaca gaaaccacac attttccgca tgcataattc tcctcttcac agatgacctg
    10681 atagccatct tccctctttt cgatatgctg caccggcgtg tcaaaataaa aatctgcccg
    10741 gtctttgagc catgcataca tattctgcag cacaatgtaa ttgacatcgg ttcccagatg
    10801 tcttacggat gcattcaaaa gctgcagacg gttctgcaga caaagttttt tgaatttcgt
    10861 tccggctgtt gaatacagtc tggtgccttc accgccgtac tccatgttaa tctcatcgac
    10921 atactgcatc agctccagag ctttctcttt gccgatatat tcataaagtg tgccgccaaa
    10981 atcattggta atattatatt taccatcgga aaatgctccc gcaccgccga atccgctcat
    11041 gatcgaacag ctcttgcagt gaatgcagct tttgatcttc ttgccatcaa tcggacattt
    11101 tcttttttcc agtgcatgtc cttcctcaaa aatggcgatc tccagatttt cgttattttt
    11161 cagaagctcg tacgcggaaa aaatgcctcc cggacctgct ccgataatga ttacgtcata
    11221 ttttttcata ctgtttcctc cagattgtac ttttaaaata tggtgttgag gtcaaaacct
    11281 atcaacttat gagcaagctc atgtggattt tgaccttttg cccctggtat catacataat
    11341 tctctacgat cttcatgatc ttcttcatgg ttttcagggt atcttttatt ttttcatgtt
    11401 ccatactgtg tccgacatcc ggaaaacaat accataaaac ctcatgtttt ttgcagaatt
    11461 tttttaattt tttccgttcc agatagtgat ccagctcccc gcttatcaca atgcagtttt
    11521 cctttcgcat gtacggaaag gtctgctcaa gcggtgtcat aaagatatga cggatcgttc
    11581 ccggttttac acgtttttcc ttttccagtg catccgcaag ccatcctgca gataccgttc
    11641 ccagactttt tgacacaaaa agaatctcat cgtacgaagc aagatctttt tctttgatcc
    11701 gcccacgcac ataagaaagc gtctgtctga tattctcttc gatggtgatc atgtcttttt
    11761 tgtcccggta ttccgaatag tgcatcagca gtgtatcgta tcccttttcc cggcagatcg
    11821 actctgcata ataaagcagc gggcagtcta cgccatagcg tctccccgga aaaaatacgg
    11881 ccagtttctt tttcattttg caatgctccc tgtcacgttc tcaaattatg cattctattt
    11941 tatcatagaa tctgctgttt ttcaaaccag attcgtatag aaagaagaaa aaatccacgg
    12001 cagttgtttt ctgccgcaga tcttctgttc tttcaaaggc ttctgagata ttgatttctt
    12061 tatgcctgta cctcttttat cacattcata aatgcctgca tctgcggcga aatccattta
    12121 tctctgtgat ataagagctg tttccatata tcaagctgaa aatctttcac atccagccgc
    12181 accagtcttt tttcctgtac ggccttttct gtcacataat ccggcagaag cgagattgcg
    12241 gaagcgttct tttccagcat ccggcagatc agttctgtat ttcccacctc aaaaaccggc
    12301 tggatctcca gggaacggct cgccagatat tcacttaaca tcctgcggta gctcatttct
    12361 ttttccgtga ggataaacgg ctggcagatc agttcctgca acagaatctg cttctttccg
    12421 ttcaagctat tctgatatcc tgtcacaaaa tgcactgctt cctggctctc tgtgacgatc
    12481 acataatcac tgtggtaaat gtgacggtcc agcgtgtata caaaatccac ctgattctga
    12541 ttcaaaagtc ggaacatctc atccgtgccg gcgatcacga ttttcatcgt aacctccggg
    12601 tacttccgct gcaaaagaac aaacagtttt tctgcaatct cctcgcacag cgaatccgac
    12661 atggcgatcc gcagatgtcc acgcacttcc tgctttggct gcaccatctc ctgcatctca
    12721 ccggcaagct gcttcatgcg atgggcgtaa cgcaggattt ccttgccccg gtctgtcagt
    12781 gtcactgtgc ggttgatccg ctcaaaaagg acggtgccaa gctcctcctc caactgccgg
    12841 atctgaaatg agaccgtaga ctgtgagtag cccaaaagct cggcggcttt tgtgaaactg
    12901 ccaagttcgg cagtgtaaat aaaggtgtta atgtttttca tatccatgat tggctctcct
    12961 tgtatcaaaa ggcggaacaa tattttttcc attgtcactg attaatcggt tttttcgatg
    13021 gtttaaatca ttatcattca ttttacaaat ctatataagt gaagtatact atatttcacc
    13081 acgagatgct accattatta gtcattttga caacaattta agaatatcag aggagcaatg
    13141 gaattccgct gctgatatta atttgttgct cgccgcctgc aaaggcgaca tcatttcata
    13201 tctgctacag gcatctaaga ttttacaggg ggatttttat tatgaatatc attaccgcag
    13261 tctacaattt tttatggggt gatctgatca cgctgccgct tccgggcggc agttcgatcg
    13321 gcatctcact tctggtgctt cttctggttc cggcaggaat ctattttaca atccgcacca
    13381 ggtttgtcct gatccgcaag tttccgcata tgatccagat cgccatggag aaaaacgaac
    13441 attctcaggc gggcagtatt tccggatttc aggctcttat tgttgccact gccacccgtg
    13501 tcggcatggg caatctggtc ggcgttgtcg ctgctatttc tgccggcggt gccggtgcgg
    13561 tattctggat gtggcttctt gctctgcttg gctcctccac tnnnnnnnnn ntccactgct
    13621 tttgcagagg gcacactggc tcagatctac cgccagaagg atccgctcta cggcggctac
    13681 cgcggcggtc cggcctacta catccacgat tttatgacaa agaaaaaaac gaaaaagcag
    13741 aacggaccgg caaaagagaa acgttacagc ctgatcgcgg ttctgtttgc gatctctggc
    13801 ctgatctgct ggtgcggcat cagtcaggtt atcagcaact ccgtaagttc cgccatgaag
    13861 aatgcatttc acattccgcc actgtacacc acgatcttcc tcgtcgttgt ggctgcgatt
    13921 gttgttcttc gcaaaaatgc caccgttcac gtcctggatg tcgttgttcc aatcatggcc
    13981 ggctgttatc tcctgatcac actgtttgtc atcatcaaaa atatcggtgc actcccttct
    14041 gtgttccagc ggatttttga agaagctttt ggtcttcgtc aggtggctgc cggtggattc
    14101 ggtgccgtgc tgatgaacgg gatcaaacgc ggtctgtttt cgaatgaggc cggaagcgga
    14161 tctgctccat gtgctgccgc tgccgctgat gtcagccatc cggcaaaagc aggtctgatg
    14221 caggcattcg gtgtctttat cgataccatc ctgatctgta cctgttctgc actgctgatg
    14281 ttgctggctc cgtccgataa aattgccgga cttcagggta tggatctgct tcagagtgcc
    14341 atgcagtatc acctgggaga attcggtgtc atctttatcg ccgtgatcct gttcctgttc
    14401 agcttttcaa cgtttatcgg cattctgttc tatgcacgtt ccaacgttgc ctacctgttc
    14461 ggggacaact ggctgtcaca gaccgcctac aaaatcctgg cacttgtcat gctgtttatc
    14521 ggcggacttg ccgcctatac ctttgtctgg gatctcgggg atgtcggcat cggactgatg
    14581 accatcttca atatgatcgc actgattcca ctgtccggac aggcgatcga ttccttaaaa
    14641 gattacgaac gcaaagaaaa ataatgccgc ctcatcttta atatggagat ttgaactatg
    14701 aaagacgaaa aatatctgga actgctcgcg gaaaaatatc cgacggagca ggctgtatgt
    14761 cgggagatca tcaatctgaa agcaattctc ggtcttccaa aaggcacgga gcattttatg
    14821 agcgacctgc acggagaata tgaagctttc tgccatattt tgaataactg ttccggtgtg
    14881 atccgtgaga aagtggatct gcttttcgag gnnnnnnnnn nnnnnnnnnn nnnnnnatat
    14941 gcctatattc tggatgaact gatccatgtg cagaaggatg aggacgacaa tcaggttgcc
    15001 tatcatcaga atatcatcga tactttgctg gaacttgaca gtgccgatgc atttatcgaa
    15061 gtgctcgccg ccctgatcaa acgcctcgcc gtcgatcatc tgcacattgt cggagacatt
    15121 tttgaccgcg gaccatgtgc cgaccggatc atggatcttc tgatgaatta tcactctctg
    15181 gacattgaat ggggaaatca cgacattctc tggatgggtg ccgctgccgg aagtgaggca
    15241 tgtattgcta ccgtcataag aaacaattta aaatatcata acatccgcat tctggaaaac
    15301 agctacggta gcagcctgcg ggagnnnnnn nnnncctgcg tgatctgacg ctgtttgctg
    15361 aaaagcttta tccggataca gagccgatgg aagcagcatt aaaggccatt tctgtccttt
    15421 tattcaaact ggaagggcag gtgattcttc gaaacccgga ttatcagatg actgacaaac
    15481 tgctgctcca ccaggtcaac gttcaaaatc acaccgtctg catcgctggc acagactacg
    15541 agatctgcga agaaactttt ccgacggttt cctttgatcc ttccaacctg gaagtttctt
    15601 acgaactgac cgccgaagaa aagcagataa tggaagggct tcgcatggca tttgtcggca
    15661 gtgtccgtct gcgtcagcat atggattttc tctatcagaa aggcagcatg taccgcattt
    15721 ttaatggaaa tctgctcttc cacggctgtg tacctctcga tgaaagcggc aatcttgaag
    15781 gtgttgtatt ccaccagaaa cgttaccgcg gacgtgatta tctggattat gccgagcgga
    15841 tcgccagacg tgcctggtct aaagatgcca cacaaaaaga actggatttt atgtggtatc
    15901 tctggtgcgg cagaaaatca ccgttgtccg gcagaaatat caaaacgttt gagcgtactt
    15961 atgtaaaaga tgaaagtatc tggcatgagg catccaaccc ttattatcag tactacgagc
    16021 aggaaaaaat ctgcaatatg atccttcatg aatttaatct ttattctgat cggtcacata
    16081 tcatcaatgg acacaccccg gtacgcacct ccagagggga acacccggta cgtgcaaacg
    16141 gccgtctgat ggtgatcgac ggcggattct gcaaaagcta tcacaaaaca accggtatcg
    16201 ccggatacac gctgatcttc aattcgcacg gtatccgcat caaatcccac cagccattcc
    16261 agagcgtcta tgccgcactg accgaaaata aagacatcga atccagatct gaactggtcg
    16321 aaacggagcg agaacggctg atggtacgca acaccgacac cggcaaaaag attctggaag
    16381 atattaaagg attgaaaatg ctgcttcagg cttaccgtga aggcacaatg gaataaattt
    16441 ttcgggtgtt ttgacacccg aattttttta tgttacactc tacgtgatgc ccgcaagcca
    16501 cagcaagaat gtcaaaggca ttcttgaatc cgttttgttg cttccggcat catgacatta
    16561 tataatacgc tgtttggaat attttcccgt atttcgggat tctgaacgga cagactcaga
    16621 caaaggagat tcaataatgg gaggactatt tggagttgct tcaaaaacag attgtatcat
    16681 ggacttgttc tacggagtgg attatcattc tcacttagga acacgccgcg gcggtatggc
    16741 tacgcatggg gcggacggtt tctgccgctc gatccacaac atcgaaaatt ccccgttccg
    16801 tacaaaattt gaacgcgatg tagaagaact gaccggaaat ctcggcattg gctgtatttc
    16861 tgactacgaa ccgcagccgc ttctgatcca gtcacatctt ggaagttttg cgatcacaac
    16921 ggtcggaaag atcaacaatg cggacgaact tctgaataag atttacgaaa acggtcattc
    16981 acattttcag gagatgagtg gcggtcagat caatgccacg gaactcgttg ccgcactgat
    17041 ctgcaagcag gacagctttg agaacggtat ccgctatgcc caggaagcca ttgacggttc
    17101 cctgacactg ctgatcatga caaaagacgg catttatgct gccagagata tctacggaag
    17161 aactccactt gtcatcggac accgtgaagg tgcttactgt gcagctttcg agaatttttc
    17221 ttacattaac cttggctaca cagatgtaaa agaattaggt cccggcgaga ttgtatacat
    17281 caccccggag agtgtggaaa cgctcgttcc gcctcgcaag aaaatgaaaa tctgttcttt
    17341 cctgtgggtt tactatggtt atccgacttc cagttacgaa ggcgtaaacg tagaagaaat
    17401 gcgttataac tgcggtgcac tgctggcaaa acgagataaa ggaaaagtag atccggatat
    17461 cgtagccggt gtcccagact ccggtatcgc acatgctatc ggatatgcca atgaatccgg
    17521 cgttccgttc gcccgtcctt tcattaaata tacaccgacc tggccgcgtt cctttatgcc
    17581 gacggaccag cgtcagagag aactgatcgc ccgcatgaag ctgatcccgg tacatgccct
    17641 gatcaaaaac aaaaagctgc ttctgatcga tgactccatc gtccgcggta cacagcttcg
    17701 tgagacaacc gagttcctgt atcagagcgg agccaaagaa gtccacgtca gaccggcgtg
    17761 tcctccgctg ctctacggat gtaaattcct gaacttctcc cgttccaaat ccgagctgga
    17821 tctgatcacc agaagagtga tcctggaacg cgaaggaaaa gatgcagaga aatatctgga
    17881 agaatatgca gatccgaaca gcgaacgtta tcaggcaatg ctcgatgaaa tctgccgcat
    17941 ccagaatttc accagcctgc gttatcacag actcgatgac ctgatcaagt caatcgggat
    18001 caagccgtgt gatatctgca cctactgctt caatggagag gagtaggtag c
//
LOCUS       DAWDWH010000015        18019 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_1823_length_18019_cov_11.674015, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000015 DAWDWH010000000
VERSION     DAWDWH010000015.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 18019)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18019)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18019
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1823_length_18019_cov_11.674015"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    215..224
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10463..10472
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11301..11310
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13870..13879
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6024 a         3404 c         4654 g         3897 t
ORIGIN      
        1 ggagcaaaag aagaacatgc agattggtat ttctttaaat acttagaatc tcttaaaata
       61 gaagaccact atactttttc tgttggagaa actgaaatta cagttccggt tgatgtattc
      121 acgccatttg ttgaaattga aaccgaaggt agagtgatta atccacaatg gcacttgatg
      181 attgcttact atgcttatat tgatgcaatg tatannnnnn nnnnaatgat aatgagtatg
      241 cgaaaaaaat aaaaacaatg ccaggacatc caactggcaa atttggtatt cgatgtaagt
      301 cattaaaaaa gtggatggaa gaagcatttg gaaaacacca gaaaggaaag caaaatggcg
      361 aaaaatcagg attttgaaaa agattttgtc agactgaaaa atgtttcgaa gatctaccga
      421 atgggtgaag ttgagatccg tgcggttgac aaaatagaat ttacgatcga caaaggagag
      481 tttgtggtcg tggtagggcc aagcggtgcc ggaaaaacaa cggttctgaa cattcttggc
      541 ggaatggata cggtgacttc aggggatgtg ctggtcgatg gagagaatat tgccaaatat
      601 tcccagcgga agctgacagg atatcgcagg gatgacattg gatttgtttt ccagttttac
      661 aatctggtgc cgaacctgac agcgaaggaa aatgtagagc tggcacttca gatctgcaag
      721 gagccgcttg atgcggtgca ggtattaaaa gaggtcggac tgggaaaccg tctcgacaat
      781 ttcccggcac agttgtccgg tggtgagcag cagagagttt ccattgcaag ggcacttgca
      841 aaaaatccaa agcttctgct ctgcgatgag ccgaccggag cactggatta tcagaccggc
      901 aaagcgatcc tcaagctttt gcaggatacc tgccggcaga aaaaaatgac cgtgatcgtc
      961 atcacgcaca attcggcact gacgccgatg gcggatcgtg tgatcaagat caaaaacgga
     1021 aaagtatcga aaatgtatct gaacgaaaat ccagaaccga tagaaaacat agagtggtga
     1081 tagaatgaaa aaacgtgcac ttcgaaaaga attttttatg gagattcgaa aaagtcttcc
     1141 gagattttta tcgattttgt ttatcgtggc acttggaacg gctttttatt ccggtattca
     1201 gtcgtcctca ccggatatgc gttattcggg ggatgcttat tttgatgcaa aagaactctc
     1261 agatattcag gtcacaggca caatgggtgt gacggaggac gatgtggagg cactgcagaa
     1321 cttaaaagac attgaaacgg cggaaggtgt ctgtctgaaa gatgtgctga caggggaaga
     1381 aggacagcgg aaagtgctgc gtatcttatc cattccggaa aaaagcaatc tggtgacggt
     1441 agaagaaggc gagtacccga caaaagagga cgaatgtctg atggatgttc agtatgcaga
     1501 gcacaacggc tatcaaatcg gcgatacctt ccggatcagc gaagagctgg aaaaagatga
     1561 ggatgccact ttaaaatcgg acaccttcac gatcacggga ctttgcagca gcccgctcta
     1621 tatttcgttc agccgcggca gcaccaatgt cggaaacggt gagattgcag gatttgtcta
     1681 tgttgtacca ggcgtcagga agaaataaaa gacgaagctc aggaaaaagt gagcgatgcc
     1741 agaaaagagc tggcggacaa aaaggctgag gcggatcaga agcttgccga tgcgaaaaaa
     1801 gagatcgatg atgcaaagaa gaaactgaag gacggcaaaa aagagcttgc cgataaagag
     1861 aaagaattaa acgacggcaa aaaagagctg gcagagaaag agtgggaact tgccgatgca
     1921 cagcagcaga tcacagacgg acgcacacag cttcagagtg cggaaaatca gctggcggaa
     1981 aaagagagtg aatttcagaa gcagaaggca tctgcagaag aacagatttc cacaggagaa
     2041 aaagagcttg caaatggttt gaagagttta cagaaaaatc agaagacgtt tgcacagcag
     2101 aaagcagctt ttgagcagca gaagcagacg tatctggacg gtgtggctca gtatgaggaa
     2161 aatcttgcca agtacgaaaa cagtaaaaaa cagggcgttc cggaagcaat gctgacgcag
     2221 gcaaaggcag agcttgatgc aacaaaaacg cagcttgatg cagccggggc acagatcaca
     2281 gccggggaaa agcagttaaa tgatgcacag aatcaacttg atgcggcaca gaaaaaactg
     2341 gatgaaaatc aggcgaagat tgatcagtca aaacaacagt taaaagaagg tgaggcacag
     2401 ttaagtgcag cgagaagcca gttaaatgaa aaaaaagcac agttaaacgc ctcacaggca
     2461 cagcttgacg atgggaaacg gcagattgaa gatgcaaagc agaaattaga agacggacaa
     2521 aaacagatcg aagatgcgaa agcgacgatc gcaaaaaatg agaaaaagct tgcaaaaggc
     2581 gaacgtaaat acgaaaagaa aaaggcaaaa gcagagaaaa agattgcaaa agcggaaaag
     2641 aagattgatg acgcacaaaa agaagtggat gatatcaaag aaccggaatg gacggtcgaa
     2701 gaccgcagtg atctgctgga gtacaccaat tacggagata atgccgaacg aatgaagagc
     2761 atcggggaag tgttccctgt tcttttcttc ctggtggcag ccctgatcag cctgaccacg
     2821 atgacgcgaa tggtcgaaga agagcgtacc cagattggaa cgatgaaagc actcggctac
     2881 agcaaagtgg acattgcggc aaaatatctg ctgtatgcac tgtttgcgac ggtcggcggc
     2941 agtgtccttg gcgttctggc aggggagaaa atattaccgt atattattat tcggtcttat
     3001 ggtatcatgt atttacatat gaatatgata tgtattccat ataatatgaa atttgctctt
     3061 atcgcagccg gagcggcaat cttctgtacg atgatcgcga cgctggaagc atgcatacga
     3121 gagcttcatg catggccggc cgttctgatg cgtccgcctg caccaaaaca gggcagacgt
     3181 gtctggatcg agaaaattcc gtttttgtgg aaacatttaa gttttacctg gaaatcaacc
     3241 gtacgcaatc tgttccgtta taagaaacga ttcttcatga caattatcgg aatcggcggc
     3301 tgtatgggac ttctgctggt tggattcgga ctgcgtgatt ccattatgga tgtggcaatt
     3361 ctgcagtatc aagatattca ggtttacgat ggcatgattg ttctggatgc agatgcagat
     3421 cagagcgggc aggacgaact gaaacaggag cttgccgatg acggaagcgt ggaagcgttt
     3481 gcagaagtat ccatgaaaaa ggtggagttt gcgtccactg acaaaaaact ggatgtctat
     3541 ctgatggtcg tgccggatga tgtgtcgatt gatgagtttg tgaaattccg cagcagaacg
     3601 acaaaagaag cctatgagct tgatgatcag ggtatgatcc tcaccgaaaa agcaggaaaa
     3661 ctgcttggcg tttcggtagg agattctgtg gagatgacag gagaacacag cggtgtaaag
     3721 attccgatca acagaatatg tgaaaactat atgtcacatt ttgcatatat gactgagaat
     3781 atgtatcgga aactttacgg agaaaaacca cagtacaaca gtattctcta ccgtgtgaca
     3841 gaccgggatg agagcatcgc agaaaaagca ggcgaacgtg ccatgaaatg tgatgcggca
     3901 ctgagtgtca cttatacggg caacacaaaa gaacagcttg ataatatgct gactgcactg
     3961 gattccgtga tcggtgtgct gatcctttct gcgggtatgc tggcatttgt ggtattgtac
     4021 aacctcaata acatcaatat caatgaacga aagcgagagc ttgcgaccat caaagtgctt
     4081 ggattttatg acaaagaagt atcagcttac gtttaccggg aaaatattct gctgacgatc
     4141 cttggaagta ttttcggaat attcctcggt atactgctgc accgctttat cattgtgacc
     4201 gtggaagtgg atgtctgtat gttcggacgg aacatcagcg tgttcagtta tgtgatatcc
     4261 gccctgatca cctatggatt ttccattctg gtaaacggtg tgatgtattt caaactgaaa
     4321 aagatcgata tggtagagtc actgaaaagt gtagagtaaa agataaaaac aaaaaagaag
     4381 gagaacagaa ggatgtattt gccggaggca tttcagaaaa aaatgaaagc aattttgggt
     4441 gaggagtatg atgattttct ggctggattt gaaaaaccgc gtcattatgg cctgcgggtg
     4501 aatacagcaa agatcaccgt agaagaattt aaagaactgg tgccttttca tctgacacag
     4561 attccgtggg tggaaaatgg attttattat gaagaggaag atgcaccgac cagacacccg
     4621 tactattatg cagggcttta ttatattcag gagccgagtg cgatgacgac tgcctcaaga
     4681 ctcccggtag gtccggggga gcgtgtactg gatctctgtg cggctccggg cggcaaagcc
     4741 acggaactgg gggcaaaact gtttggggaa ggacttctgg tggcaaatga catcagtgct
     4801 tcgagggtga aagcacttct gaaaaacatt gaagtgatgg ggattccgaa ttcctttctt
     4861 ttaaatgaag tgccggcaaa gatcgccgaa cagtttccgg catattttga taaaatcctg
     4921 gtggatgccc cgtgttccgg ggaaggcatg ttccgcaaaa atccggaggc ggcacgcgtc
     4981 tggagtgaag aaaaaccgcc ggaatgtgca aaaatgcaga gagagatcgt aaaacaggcg
     5041 atctccatgc tgaaaccggg cggcatgatg atctattcga cctgtacctt ttcaccggaa
     5101 gagaatgagc agatcatcgc atgggtgctg cgtgaatttc ccgaaatgca gctgatcccg
     5161 atgaaaggat acgagggatt ttccgaggga agaccggatc ttgcagacgg caatccggaa
     5221 ctgaaaaaat gtgtacgtat ctggccgcat catatggacg gcgagggaca ttttgtggct
     5281 ctgatgcaga agaggcggac gccggaaatg gacgatattt caccggaaaa agcatccgca
     5341 tatatttcgg aagcagacga agagccatcg gatcatgatg atgtaaaaaa gaaaaagaaa
     5401 aaagcaaaaa aaggacgaaa agatcagaag gaaagaaatg aagcggcagg atgcaccaga
     5461 caggaaaggg ccgttctgga gagctttttt gccgatgtaa aggcagaagt tgactggaaa
     5521 cgcatcgagg tgcgaaaagg gtttgcctat tacctcccgg aaggcgtgga aggtaaaaaa
     5581 aatctggttt ttgtgcgaaa cggactctat cttggggaga tacgaaaaga ccgcttcgag
     5641 ccgtcacagg catttgcgat ggtattacag aaaaaagaat ttgcctccag tattgatttt
     5701 ccggcaaagg atgagcgagt gatccgctat ctgaaggggg aaacggtgga tgtctcggat
     5761 ctggagtgtg gaaaagagaa aggctggcag ctggtctgtg tggatggtta cccgctcggc
     5821 tggggaaaac tggtgaatgg gacactgaaa aataaatatc tttcaggctg gagaatgaaa
     5881 gtgaatggat agctaaaagg agaggtggaa gaaagtgaat tacagggttg tgctggcaga
     5941 tgatgaaccg gaggttttgc ggagcataca gagaacactg gactgggaaa agtatggctt
     6001 ctctgtagtc ggaggatttc aaaacagact ggatgtactg gaatttctgg aaaatcacga
     6061 tgcagatatc gtatttaccg atatccggat gccgtttatg gacggaatcg aactgatgca
     6121 taaaatcaaa gaaaagtatc catacataaa gctggtgatc atttccgggt atgatgattt
     6181 ccagtatgca aaagaagcac tgatccatgg cgttttggat tatattctaa agccgatcaa
     6241 tgccagggaa atgtcagaag ttcttcagaa agtcaaagac aaaatggata ccgagctgga
     6301 agaaaagcag agcatccaga agcttcgaaa actgtatctg gaaaatcagc cgatcataag
     6361 ggaaaatttc ctgaaccggc tggtacttgg aaatatcaag gcaaaagcac ttcaggaaga
     6421 aaaaaagaca gggctgatcc gcatgggaga atataaatac tgggcaacgg cactggtaca
     6481 gatttatcag atcgaacaga aagaccatgg agaaaccatg gacagccagc tggcattcat
     6541 ctacatccgc aatctgatcc gaaattccat atcggatgat atttattatg aagcttttta
     6601 caatccgctc ggggaatgta ttctgtttgg tatgaatgaa ccggaagaaa tggaaaagat
     6661 tcttttaaag ctcaatgaca ttgcaaaaga gagcaaacgt gtgatgaaga tcaaggcagc
     6721 gatcggtgtc ggaaagatca aagatgagct gatcaaagtg aaagaatctt ttgaagaagc
     6781 caaagaagca ctttcctacc gcaaaatgtc gcaggacgga gatgtcattt atatggaaga
     6841 tattgacaaa tccgagcagg tgatcctgtt gtttgatgag aaagcacagg acaagctgtt
     6901 cacggcagta aaattcggaa aagaagaaga tattcatgcc gtgatcagaa aaatgaaaga
     6961 atatctgatc aaagccgata tgagcagcag cagttatcag gcgtacagca tgagcgtcat
     7021 caatgcactt cttgtttttg caagacagca ggatcttgag attgaagaag tctttgacgg
     7081 gattccgaat tatgtggaga tcatgcagaa atatgcggat gccgatactt ttatggaatg
     7141 gctggaagag cagtgcatcc gtttgaatgc cagccagggg cgggaacgtg agaacaaggc
     7201 gaaaggtatc attgaagagg caaaacagaa aatccaggaa gagttcggag atccggatat
     7261 aggactggaa aagattgctg ctgaagtcgg actgacacag acctattttt ccagcctgtt
     7321 caaaaaagag accggcatga gtttcgtaga gtatctgacg gacaccagaa tgaaagaagc
     7381 catgcggctt ttaaaagaga caagtgaaaa aatctatgta gttgcccaga aagtgggcta
     7441 tctggaatcc ggatatttca gccatgtatt taagaaaaaa tatggaatgt ccccgatcca
     7501 gtgccgcaga caggggcaat agatgggagg ttgtatgtgg gggaaaatca gacaggactt
     7561 tcggaagttt ctgccgaaac agagtatgca gaatattctg tatgtgatca ttctgacgct
     7621 gacgctgctt gtcgctgtct ttgtaggatt ttttgtctca aagtctcagc aggaaaagca
     7681 ggcacagatc atcgttcagg ataatcagga gctggcagaa cagatcaacg tatctatgtc
     7741 acagtatctg cacagtatga tgcgtctgtc agatacgctt tattataata ttatcaaggg
     7801 aaacgacagc ggacagatgg aacagatgtt tcaggcgatg tatgacggct acaaagatta
     7861 tgtggagagc attgccctgt ttcaggagga tgggacactt ttgcaggtca tgccggcact
     7921 gtcatctgcc tcttcctcag atgttatgca ggaagaatgg ttttccagtg cacttgagcg
     7981 aagtgagaac attcattttt tcagaccgca gatacaggac tgctttgaac ataacagtag
     8041 ttttccgtgg gtgatcccga tgagccgttt tgtgcagatc acgcacggaa atcaggtgct
     8101 gagcggagtg ctgctcatca atataaaata cagtgcatta tctgaagttt tcaggaactc
     8161 aatagatgat atgaaccagt attcttttct gatggattca aacggagaac tgatctacca
     8221 tcccaggcac gcccttgtaa acagtgggat tatagaaaaa ccgccagaaa cactggctga
     8281 atatcaggat ggatcctatg aaacagaaat cgggaaacag caggtgttct gcagtgtcaa
     8341 gacggtggga tacaccggat ggaagatcat tcgtgtgatc gggaaagaag agtttagaaa
     8401 ttatggaatc aaaagtactt tttttgtgct ggcggtaatc tgccaggcag ttctgttttt
     8461 ccagattgtc ggatcgtatc tttccatggt gctgacaaga ccgatctgca acctggagga
     8521 agatgtcaaa cgaatttcag ccggagagct ggatatcaaa gtccatactg ccggaagttt
     8581 tgaagtgtat catctgggac ggtctattca gaagatggca gtcaggatca aaaaactgat
     8641 gcgggaagtg atccaggaac aggaagccaa aagaaagagt gaactggaca gccttcaggc
     8701 acagatcacg ccgcattttc tttataatac gctggacatt gtagtgtgga tgatcgagga
     8761 aaaccgaaaa gaagatgccg caggcatggt tacggcactg gcaagatttc tgcgtatcag
     8821 cctcagcaaa ggaaaaaata tcatcacggt agcagatgaa ctggaacatg caaggaatta
     8881 tctgatgatc cagtcacttc gtgcaaaaga tcagtttacg taccggattg aaaaagatcc
     8941 cggcacagaa tgttttcagg ttatcaaact gattgttcag ccaattgtgg aaaatgcact
     9001 ttatcacgga ctggaaggga tgtacggcga tggtgagatc gtgattcatg cctatacaaa
     9061 agacggggat ctctacattt ctgtgaaaga caacggtatg gggatgtcaa aagaacaggc
     9121 agaggcactt ctggattaca cgaaagaatt aaagacggca aaaggaaacg gcattggtgt
     9181 acgaaatgta cacgagagga ttcagctgca ctttggaaaa gaatacgggg tacagatcat
     9241 ttcagaggaa gatgagggaa cagaaatcct gctgcatctt ccggcaatta tgtatggaga
     9301 aaagaaagaa tgagaagggc aaagaaaatc ggtattgtgt gcgtggggat tctgatggtt
     9361 gtttttatgt ttctctcgta ttcaaatttg tttggaggag agacggaaag agatatcaca
     9421 aaaatttctg tgatcgggat caagagaaaa aacagtcagg tagaagattt taaaagaggt
     9481 attgaggaag cagcaaatgc aaagcgtgtg gatgtcgagt atctggagat agaggaagat
     9541 tatgacacga tcaaagagta catagaccgt gagatcaaaa acggctctca ggcattaatc
     9601 gtgttttcag aaaaagaaga acagattctc tcttatctgc agtcagaaaa gaagaaaatc
     9661 ccgctgatct tggtgaatcc gggcggaaca gaagaaaaaa cgatccgtgt ctggtttgat
     9721 gtgcaggata tggcagaatg tctggcagaa aaaatccatc agaagcgtca ggaagaagag
     9781 ccggtggtat atttaacgga tgagaaagaa atgtcggagc gaatgtggac aatctttcag
     9841 gaaacatttc agaagcaggg gattgcaatc gagaaaaaga aaatgacact tccaaatctt
     9901 gcaaaatatc gggatcaaaa gcagcaggct gtgtttatct gcagttctcc aaagatcaca
     9961 gaagaggcat cagactatct gaaaagcaaa aaccaggtca tttatgggat cggttattca
    10021 gacgaagtgc tgcaggggat ccgggaaggg aaagtggaag gtgttatggc atacagcatg
    10081 tattctttcg ggatttatgc agtgaagtct gctatttact ctatagaagg aaagaaagtg
    10141 gatcatctca caaagacaaa atgcgagtgg atcacgcagg acaatataaa agaaaagtat
    10201 gattttctgt ttccaattta ttgataaaat cgcaggagtt aatgtccgct ttacaaggtg
    10261 gaagaaggag gcaaaaacaa atggggtcaa aaaaggaact gcaggcagcc atcgcagtac
    10321 ttggtctgtg catgctggtc tttggatgta caaagacagc ggagagtgag aacagcaaaa
    10381 agcaggtcaa gatcggggtg gctgtataca acgaagaaga tgtttatgtc agcaagatct
    10441 gcggttatct cgaggatgaa atnnnnnnnn nnactatgat cttctgctgg tcaatatcgt
    10501 ggatcgcacc aatgcggctg tgatcatcga caaagcgacg caggcggaca ttccggttgt
    10561 gtttttcaac agagagccgg tgcgggaaga cattttccgg agtgatcaga tttattatga
    10621 aggatcggat gcaaaacagt ctgctatttt gcaggcggat gtaattgcag atgcactgga
    10681 aaaagaccgc agtaaaatcg accggaatgg tgacagcgtc atacagttcg ccatgctgga
    10741 aggagagtcc ggtcatcagg acacgctgat ccgttcagag tgggtgctga aagagctgga
    10801 aaacaaaaaa atcccgacgc agcagatcgc aggcagcacc ggaaactggg aaaaaaatca
    10861 ggcgaacgtg atcgtacggc agtggatgaa agagtatccg aaccagattg aagtgatcat
    10921 cagcaataat gatgacatgg cactgggagc ctgggaagca ctcgaagaaa aaggatgcac
    10981 cgaagtgcag gtggtcggaa tcgacggaat cgaagaagta cgcgaactgg tggatgaaga
    11041 taagatcctc ggaagtgttc tgtgtgatac aaaactgcat gcaaaagcac tgatgcagtt
    11101 catagatgtg ctggcttttc ataacggcag tgtagagaaa ctgaaccttg aaaatgaaag
    11161 atattatatg attcctctta caaaagtgga aaaatagaaa aaagttagaa aaaatctaaa
    11221 aaaatttcaa ccaaatctaa taaaaatcta tggaaattta ccgagaaatc agataaaata
    11281 gattcactaa ggcacagaaa nnnnnnnnnn gtaaaaagaa tgaagaaaag taagattttt
    11341 tctgtagtat tggcgatgtt tttgattctg acactgaacg ttggagctgt gagggcagca
    11401 gataccgcca gaatttatct ggatgccgtt caggcaaaga gtggtatcgt gaccgtttcc
    11461 tgtaaagcag agaacgtggg caagatcagc aacggaaaat taaggattac atacgcagcc
    11521 gacaaactgc atctggtaaa gacagaggca ggagatggac tgaaaaaagg taatttccag
    11581 acggttatca acgatccgat cgacggaaac aaagcagaag gtgagatcgt gtttgtattt
    11641 gcttcttcca gagagcagga agtcagcggc aatctggtga ccatgacatt ctccgcaagt
    11701 gagaaacttg atctcaaaga tacaggtctg aacctgaaag cagaagagtg gaacaaaaca
    11761 agcggatcgg atattaatgt cagcatcaaa gatcttcagt acagaagcga agtgatcgac
    11821 aagattgagc cgacaccaac accggaaaag aagatcagcc tgaatgatac acagatcgcc
    11881 gaaatcgaag atcagactta cacaggaaga gccgttcgtc cgggtgtgac gatccagtat
    11941 cagggaaaaa cactgacaga agccaaagat tatgcactga cttacaaaaa gaataaaaag
    12001 atcggaaaag caagcgttac gattaaaggt atcggagaat acgaaggaag caagacgatg
    12061 accttctaca tagcaccgag accgccaagg atcaaaaaag cagtttcaga ttccagaaag
    12121 aaagtatccc ttagctggtc caaaaagaaa caggcagacg gctaccagat caagatcgca
    12181 cgcgacaaac agtttaccag gaaagtaaag accgtcaaca tcaagaaaaa cacaaaagtg
    12241 aaaaagacag tcaaggtaaa aggaaagggc agagcaaaat attatgtaag aatccgttcc
    12301 tacaagatca ttgacggtaa gaaacattac ggaaaattcg gctataaaaa agctgtccgt
    12361 ataaaataat gcagtccaga ggaggcaaaa caaaccatga aaaaagtatt atgtgcgatg
    12421 atgctgtttg cagcagcaat cctgtgcctg catcagaatg tattcgcagc tgagggggca
    12481 accgtaacgg taaacggtga cacacttcag gcaggaagtg atgcgtggaa agttaccctt
    12541 gatatcagcg gcgatacgaa aattacaaac ggcaagattc gtgtgacgta tgacagctca
    12601 cagctgaagc tggtatcaac ggaagcaggc tccatgatga atggcgttct gaccgacatc
    12661 aacgatccgg tcagcggcaa taaatccgaa ggagaagcag tgtttgtatt tgcatcctct
    12721 acagaactgg atacaaacgg aacacttttt gaaatgactt tccaggtaca ggattcgctt
    12781 aaagacggtg acagcgtaac ggtttccgca aaaacggaag aactgcagaa taacaatgca
    12841 gccgttgccg tgaccgatgt accgctgaac gcagcggtgg gaagccagag taacaataac
    12901 ggaaacaaca atggcggaaa tgattcctcc acagaaccac agaccgagac agcatccaat
    12961 aatggaaaca acggaaacgg aagtggatcg gataaccagg gcggctccgg tatccagacc
    13021 gaggcacctt ctggaaacag caatacctcc ggtggcagca gcaccttcgg cggaacatct
    13081 tccggaagca gcagtactgc gggaacccag agcggaagca gcacggttac atccgcaaaa
    13141 acaggagata acaccaatgt tttaagacca cttatcatcg cagtgatcgc actgatcgtg
    13201 ctgattggcg gatatgtatg gtccagaaag aaaaagaaat aaaagccaat acgaaatttt
    13261 taagacagga agtgcacttg catcgagaga cagatgtgag tgcatttttt atcttagtcg
    13321 gagaagactc ccacttttat aagtggcgag caacaaattt gtcttagtgg gagtgcaata
    13381 tacctgcgat gtaccggaag tgcgaacaga tagtgtatct gttgtatcaa aaaatgtgga
    13441 aaatgtataa aaaatatata aaacaagaaa atacatacca tattttattc aaaataacga
    13501 ttaaaaacag aggactaaca ggaaacataa aaatttacaa gaaaaacgta taatattaca
    13561 tggaaaatca taaaaaagtt atataaaata aaatcacacg agaaaatgtt gatggaatat
    13621 caacatcgga aagcgaaaaa cataacaaca aagcagtaaa gcagtaaagg aggaaaagga
    13681 atgaagaaaa cttcaaaaaa agtgatagca gtcacattgt ccgcttcgat gctcttagga
    13741 ggaagcatga cagcgatggc agctacaaca aacccggaca tcagccagcg tgagattgac
    13801 cataagaccg cggctaaaaa catcgcagca cagggcatgg tcctgatgga aaacaagaac
    13861 aactcactgn nnnnnnnnng atttcagcaa agaagggaac aaaagtagca ctgttcggac
    13921 agggtgttta caacaccatc aaaggcggta ccggttccgg tgctgtaaac cagcgtgaca
    13981 atgttaccgt acagcagggt tttgaaaatg ccggatatga tatcgtaaat gcagattttg
    14041 taaaccagat gcatgagctc tggacagcaa acggcggcgg cacaagtcag ggctggggat
    14101 ttaaatgggt gaatgaaccg gtttatgaac agacagaagg tgcggcagac cagatcaaag
    14161 cagcggcaga tcagacagat actgcaattt atgttattgc acgtaactca ggtgaaggat
    14221 cagatcgttc ttccggaaaa ggtgattatc tcttatcgga tgatgagcgg gccaatctgg
    14281 aactgcttgg aaaaacattt gataacgtag ttgttgtcct gaacgtaggc ggaatcattg
    14341 atacaaagtt cttcagtgag atcaatgggc tggattccat gctcctgatg tctcaggcag
    14401 gtatgaccgg cggtgatgca ctggtagaag tattaaacgg tacagtaaat ccatccggta
    14461 aattaacaga tacctggccg gttaattttg atgacaaccc atccagtgca ggatttgcaa
    14521 acgaggataa aaataatgca caggaattgt acaacgatga tattttcgta ggatatcgtt
    14581 attatgatac gttcggcaaa gatgtttcct atgaatttgg ctatggcgga tcttatacta
    14641 cattcgcaat gcagacacag tctgtaacag cagacaaaga caaagtaacc gttaaggtta
    14701 aagtgaaaaa tacaggttcc gtatccggta aagaagtagc agaagtttac ttctcagccc
    14761 cggaaggcaa tctggacaaa ccatatcagg aactggcagg atatgcaaag acagacgagc
    14821 tggcacctgg tgaatctcag gtactgacga tcacatacga taccacagag atgggatctt
    14881 acgatacaga caaggcagca tacgtgatgg aggacggcga ttacatcatc cgtgtcggta
    14941 attcttccag aaacacacat gtagcaggca agatcagcgt agcggaagat acgatcacag
    15001 aacagtacag caatctgatg gttccggtaa aagataatcc gaacgttgca gatgatgaga
    15061 aatatccgac actcgagccg atgacaaccg aaggtgcaac accaatcaca tacgacggtg
    15121 aagcagatga gatcgcagca gcatccacga tcgcagttga tttcacaggt tacaaagcac
    15181 cggaaatcat caaagaaaac gaagatgtcg atgtatacgt atcggataca acgcagacag
    15241 aatatctgat cgcagaaaac aaagacggag caacaggcga gatcagccgt gttgaaaaag
    15301 gaaatgcaaa cagctccgac tatacagtaa catacaacga gcacgttaaa aaatttgacg
    15361 gtgatttcag caaagcgacg ctgatggacg ttaaacttaa gaagctcagt attgaacagt
    15421 ttgtatctgc actgtcactg gaagaactgg cagaactggt gaacggtcat tcaggtgatg
    15481 caaccgttaa aggtgtggca ggatctacct ggagaaacga tacaaaaggc atcacccctg
    15541 taaacctgtc tgacggacct gcaggtcttc gtattacaca gaaatacaaa gatggtgatc
    15601 agacctatta ccagtatgca accgcatggc cgatcggtac attgcttgca cagacctggg
    15661 ataccgatca gatctacgct tacggcgaag gcgttggcga agagatggaa gagttcggta
    15721 tcggatgctg gctggcacct ggtatgaaca tccacagaaa cgcactgtgc ggacgtaact
    15781 ttgagtacta ttcagaggat ccggtagttg taggtattac aggtacagct gcaacccttg
    15841 gcgtacagtc caataaaggt gttggtgtta ctatcaaaca ttatgcactg aactctcagg
    15901 aaacaaacag aaatgcagag aacaatacag tttctgaaag agcaatccgt gaaatctacc
    15961 tcaaaggttt tgaagcagtt gtaaaacagg cacagccgat ggcgatcatg acctcttaca
    16021 accagaacaa cggcagaccg gcagccgatg attatgatct gtgtaccgca tttgcaagag
    16081 acgaatgggg attccaggga atgatcatga cagactgggg cggcggacag tccgtaccga
    16141 tgtatgaaat gcatgcaggc aacgaccttg tttgccctgg caaaggtgca gctcagatca
    16201 tcaaaggctt caaatctgat ccagactgga acggaaaagg ctatgtaaat atgacaacca
    16261 tcagctatca ggatcagaca gatccggatc tcccggttat tacaaaagac gttccgaact
    16321 tcggcggata tgaactgaat aaagaaggta cagaagtaca gtctacaacc gtgaacggtg
    16381 atgcagatgc agatcacagc cttctgtgtg atgaagcatg gaaagcaatc gaagatggat
    16441 atgcaacata taaagtaaaa gatgtacgta actggtggac aggtacaaca acaaaacaga
    16501 caacggtcag ctacaaggta aacagcaaca aagatggtca gacaaataaa cagctgatct
    16561 cccttggtga ccttcagaaa gcagccatca acatctgcaa ccttgtaatg gattctacag
    16621 agtttgcaaa agaacatggt ttcacagcac agtcactgaa tgaaaaatat gcagatatcc
    16681 tgaaagatat cacttcccat tcaaaagacg ctgtacagag cacaatcgca gacaaaggcc
    16741 tgcagaaact gatcaacacc gtaaaagacc ttgattcttc cgagtataca gcagacagct
    16801 ggaaagcact ggaagatgca gtagcagcag caaaagacgt agtagcaaaa gcagacgcaa
    16861 gtcagtctga aatcgatgcg gcagtaaacg gcatcatcga tgcacttgga aacctggaat
    16921 acggcgtaca gaccctgcac cttgagacag cgatcgaagc agcagaagca atccttgcag
    16981 atgcagataa ctatgaatcc gtagatgcac tgaaagcagc agcagacgca ggaaaagcag
    17041 ttctggcaaa agcagatgca actcaggcgg aaatcgatgc agcagcagac gcagttctgg
    17101 ctgaaatgtc caaactggca gaaaacgcag acagagcttc cctgaagaaa ctcgcagacg
    17161 cagcagaaaa actgacagac ggcaactaca caagtgattc catcgctaaa ctgaacgatg
    17221 cgatcagcaa agcaaaagat gccctggaaa acggtgacga agcagcaatc gcaggtgcat
    17281 acgaagaact gatcgatgcg atcacaaacc ttgaaatgaa aggcaacaaa gcagccctga
    17341 aagctatgct ggaaaaagca gctgcaatcc ttgcagatac agatgcgtac gtagcatcca
    17401 cgatcgacgg actggcaaca gtaaaagcag atgcacagaa tgtatatgac aatgcagatg
    17461 cagtacagag tgagatcaat gcagcagtta agagcctgac actgaagatc gcagcagccc
    17521 gtctcctcgg tgacgtaaac ggcgacggtg cagtcacaac aagcgacagc gtatctgtcc
    17581 tggcagcaag tgcagaactt acaagcctgg atgcagacgc aacagcaagt gcagatgtaa
    17641 acggcgatgg cgtggcagat accctggacg cagcagtgat cctgcagaca gcagcagaaa
    17701 aataaaaaca aaagtaagat gctactgtaa aaagtatgat ctgaaagcat aagaaagcag
    17761 cacgcaaaag aatttgcgtg ccgcttttgt gtaaatcaaa atctgcggag gttttagaat
    17821 gtgcaaatga acgccataga aatgatttaa aaaaaatatg acgccatgat gattcgaaaa
    17881 caatctcctg acacgctgcg tttcctgttc ggttcgctcg cctgttagcc gcccttcgcc
    17941 taaactcgcc tcttcgaggc tcagacagcg gctctttggg gcggcagcat cgcaaacctc
    18001 acgacggaag ccttgctac
//
LOCUS       DAWDWH010000016        17676 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_1866_length_17676_cov_13.809659, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000016 DAWDWH010000000
VERSION     DAWDWH010000016.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 17676)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17676)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17676
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1866_length_17676_cov_13.809659"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    14378..14387
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4299 a         4632 c         3552 g         5183 t
ORIGIN      
        1 atcgcaccgg aaacgatcgg cagaccactt tcattgctgt ccggatgttc cttcaaataa
       61 gtgcgaagat aattctcaaa agtcattttc tgcttttcgt cattcacaca aaagctatct
      121 ccgtcctgta ccagcttctg gtatggcaga cgcccgatga tggagtagcg tcccagcctg
      181 ttttccagag aagaatccaa aaaaacgatg tccttttcct ctttccatct ggaaaataaa
      241 acatgaattg gcggatattc tctgatttcc cggatcttat gcataacaca cctccgttct
      301 ttcctgctgc cacagctgac agatgcagtg gaaattctcc agaagttcgt gaccgtactg
      361 tgtcagcacc gcctccggat gaaactgcac gccgtagatc ggatacgtgc ggtgagaaat
      421 cgccatcaca gcctgatctt ctgtccttgc atccacctgg tactcctgcg gcagcgtcgc
      481 ctcatcgatc accagcgaat gatatcttgt caccaggaaa tcctgcggca gtccccgaaa
      541 aagcccctcc cctgagtgtg tcagagggct taatttgccg tgcatcgggc gtgtcccctt
      601 tttcacagtc gcacctgcca cgtagccgat cgtctgatga ccgagacaga cgccaaggat
      661 cgggatctca cctgcaaact ttcggatcac ctctgctgaa atgcccgcat ctttcggcgt
      721 tttcggtccc ggggagatga tgattccctc cggtttcatc tgacggatct gaccggtcgt
      781 aatgccgtca ttttccacaa caagaatctc atgtccagcc tcctcaaaat aggctctgag
      841 attgtacaca aaagaatcat aattatcaat cattaagtac atagacctta cctgctttcc
      901 gcgcagtcgt tatccccgcc gaaaaggaga ggtccgtaac tcgctgttaa tttttatttt
      961 ctatatttct gatgtccgtc cagtatatca tatctccccc taaaagaaaa gaatcctttg
     1021 tgcattttca caaattctac cttgcacttc tctataccat acggtatact ggatacatag
     1081 gtttccaagc acaggcatcg ttcctgctcg caggataaga tgactgaatt tggaaaccgt
     1141 aaaaacatga ggaagtaacg atttacgcag taaatccgtg gattccgaat gtttgcagga
     1201 ttgcgaaagc tgcatggcat ctttttataa aaaactatta tatcttaaag agagggcata
     1261 ccaatgaata cactgcacac aagactttcg ggtgtacttg ttcacccgac ttccttccct
     1321 tccccatatg gtatcggaga tctcggccag ggggcttatg actttatcga ctttctgaaa
     1381 gcttccggtc agagtttatg gcaggttctg ccgcttggac cgaccggttt cggcgattct
     1441 ccttatcaga gcttttctgc ctttgccgga cagtctctgc tgatcagccc ggatgacctg
     1501 gttgatctgg aactgatcac aaaggacgat ctctatccga tcccggagtt tgatgcagcg
     1561 aaagtcgatt acggtgctgt actgaactac aagaacggtc tgtttaaaaa ggctttccag
     1621 accttccatc acacaccgaa caaattcctg ctggaagagt tcgatgattt ctgtctggaa
     1681 aacaaaaaat ggctgagcga ttatgccgta tttatggcat gtaaagatgc tcatgaggga
     1741 agatcatggc aggaatggga tgacgagatg accactccga ccgcaaaggt gcgtcttgct
     1801 tttgagacag aatacaaaga acagatcaaa tactataaat tcatccagtt cctgttcttc
     1861 cgtgaatgga tgaaactgaa aaaatatgca aatgaagctg acatcgagat catcggcgat
     1921 attccgtttt tcgtatcttt cgacagtgcc gatgtctggg caaacaaaga gctctatcag
     1981 cttgatacga aaggtcatcc gcttcaggtt gccggagttc cgccagacta tttcagtgca
     2041 accggacagc tctggggcaa tccgctctac gactggaaac accatgaagc tgacggctat
     2101 tcctggtgga tcgaccgtat caaaatgcag atgaagctga ctgactacct gcgtatcgac
     2161 catttccgcg gcttcgaagc ttactggtcc gtaccggctg atgaagagac tgctgtaaac
     2221 ggtaaatgga aaaaaggccc gggcaaaaaa ctgttcgagg ctatccagaa agagctcgga
     2281 gaggatcttc cgatctgggc agaagatctt ggtgtgatca ctccggaagt ggaagaactg
     2341 cgtgatgagt ttgctttccc tggtatgaag atccttcagt ttgcttttga gggactggat
     2401 gagagtgatt atcatccgca caatcttaca acaaccaact gtatctgcta caccggaaca
     2461 catgacaatg acacgacaac cggctggtac cagaatcttc cagaaccgca gaaagacaaa
     2521 atccgccgct actgcaactg cgacggcggc atcatcagtc tggatttcat ccggatgtgc
     2581 atgggcagca ttgcaaaata tgcgatcttc ccaatccagg atctgtttat gcaggattct
     2641 tcctgccgca tgaacacacc gggttctgct ttcgcaaact gggcttatcg ctatgaagca
     2701 tccgctctta cgcaggatcg tgtggactgg ctgaaaagta ctactgaatt attcggtcgc
     2761 ggaaggagca aaagtttatg atccgttctc tgctcattgt aattctgatc gttgcctttc
     2821 tgatcctttc catcccgatt ctgatcgttg agtggatcat cggaaagttc aacccttacg
     2881 cccgtgatat ttcttcactg cgtattgttc agtttatgtt taaactgatc ttatttattg
     2941 ccggtacgaa agtaactgtg atcggcgaag atcgtattcc aaaagataca cctgttctgt
     3001 acgttggcaa ccacagaagc tattttgacg tggttctgac ttacgcaaga tgcccgggac
     3061 tgaccggtta cgttgcaaaa gacagtatga aaaaaatccc gcttctttcc agctggatga
     3121 aacggctgca ttgtttattc ctgaacagat ctgatatcaa agaagggctg aagacgatct
     3181 tacaaggaat cgatcagatg aaaaatggtg tttccatgtg tatcttcccg gaaggcacac
     3241 gcaacgaagc atcggatgat acactgcttc ttccatttaa agaaggaagt ctgaaaatgg
     3301 cagaaaagac cggatatccg atcattccaa tgtcaatcaa caatacgaga aatattttcg
     3361 aagcccatag accgtttatc cgttctgcac atgtggttct ggaatacggc gagccgatct
     3421 atccgaagga tctgccaaag gaacagaaaa aattcctcgg tgcccactgt caagagatca
     3481 ttcagaatac catatccaaa aatcagtcac tggtataaaa atatgatgaa aaaagtttct
     3541 cagatttctt tttacaccgg gagcagagaa gaactgctcc cgggatttac ttcaaaattc
     3601 ccttatattt cttcctgttc cgatatcaca aaacatccgg gacacttctg cccatggcac
     3661 tggcatccgg ccatggagct tttttatctt aaacagggat ctcttgaata caacacacct
     3721 ggcagcagaa tccttttggc ggagggctgt ggcggtctga tcaattccaa tgtcccgcac
     3781 atgacaagac caggtccgga ttttgcttcc tcgacacagt tgctgcatct tttcgaccct
     3841 tcttttattt ccggcgtcca tggcagcctg atcgatgaga aatatgtcac accgctgatc
     3901 accgcatccc agatcgagat catccctctt tatccggatc atccggaaca taaacaagtg
     3961 ctctctgcta tccgcgattc ctttttgatt tcagaaaaca cccctggcta cgaactaaaa
     4021 ctgcgttctg ccctctcaga tatctggttt cagatttttg aacttgcact gccgctgatg
     4081 cagaccaaag accggtttga caaacgaaac aaacgcctga gactgatgat ggtctacatt
     4141 catgaacatt acgccgaaaa gatttccgta aaagatattg ccggtaccgc ttttgccagt
     4201 gaacgcgaat gtttccgact ctttcacgac tgtctgcata tgagccctgc cgaatatatc
     4261 aaaaactacc gtcttcaggc agcctgcgat atgctcgcaa atacaagaga acatatcact
     4321 tttatcagcc agagctgcgg ctttggaagc agcagttata tggggaaaat tttccgtgaa
     4381 tcgatgggat gtacaccgaa cgaataccgc acaaagctgc gtggctgata taaaatgata
     4441 gtcaggcgga tattcgcaat ccgctttgct atttggttaa aatgctgcat ttcatgcatc
     4501 atcggcagca tatcttcgat tccgcacata agattggcag tattctgcta taaaaaggca
     4561 gaaataagat atttttttgc ctttttatgc actataatca taatcgaaat gaatcatatc
     4621 cctcaaaatc ctggtcgcac acacgattgc ttaccacttt gcagaagtgg cggccttctc
     4681 cgactgaatg attcattgca gctggaaatg cccacataca tttaccttgc tgtattcatt
     4741 tcatcacact actttcggat cagggcgtga ttcggccatc atgcccgagt tcccttttcg
     4801 ttttttcgat aaatttgaga ggagattcta tgagtgcaat taaaattgtt ttttttgaca
     4861 ttgacggaac tttaattgat atgaataaga aacgtatttc tgaaaaaatg ctggagacac
     4921 tccgcaggct gaaagaaaac ggcacgatta tttgcattgc gaccggcaga agtccgatag
     4981 cacttccgca ttttgaaggt gttgaatttg atgcttttct gacttttaac ggttcttact
     5041 gtttcacaga agatgcggat atcttttcca atccaatccc gactgaagat gtacatatga
     5101 tcatccaaaa cgccacggct cttggtcgtc ctctgacggt tgccacaaaa aacagactgg
     5161 catcaaacgg caaggatgag gatctgatcg aatattattc ttttgccaaa caggagattg
     5221 atgtggctga tgattttgag caggttgcta atgaaaaagt ctatcaactg atggccggaa
     5281 gctcacttgc agaacatccg gcactgatga aggatgtttc ccatgcacgt atcaccttct
     5341 ggtggaaccg tgccgttgac attatcccga cagacggcgg aaaaggccgc ggcatcgaaa
     5401 aaatgttaga atattatcac ttcgataaat cgcaggcaat ggcgttcgga gacggaaaca
     5461 atgacatcga aatgttgaag agtgtcggcc acggcgttgc catggcaaat gcttctgatg
     5521 aattaaaagc gattgccgac gatgtgtgcg gtgatgtggc agaggatggt atttactact
     5581 actgtctgga acatggtatg atctgaactc tgcacaccgc ctgcaaaggt tgaagtccct
     5641 cacatctgaa gtataacaaa aagctccggc aggaatacag ttacattccg ccggagcctt
     5701 tttgtacacc tccagaatcc gccagaggtt gttatatccg agacgaatca tcttacagct
     5761 gacataattt ttccactact ttttcaaaat gcatgaaatg cgatgccttt accagcacgg
     5821 tatccccgct ctggatcaga gacggcagac aggtcagcag tgcctccatc gtcggaacct
     5881 gcagcacttc gccacagccg ccgcttgcca tggctgcttc tgccatatgg ctgctgattt
     5941 ttccggtgca gatcagaaga tcgatcttgt tctttgccgc atggtatccg acttctgcat
     6001 gcatctgttc ttcgttttct ccaagctccc ccatgtcacc aaggatcgcc actttacggc
     6061 cttttgcatc tcgcagcacg tccagagatg ctttcatgga aaccggattt gcattgtagc
     6121 agtcatcgat gatctggata tctccatggt taatgatatg gaatcttccg ccaaccggct
     6181 ccagtgcctc gatgccggct ttgatctcct gcatggtcag tcccagtgcc ctgccgacag
     6241 ccgtcgctgc cagtgcattg agtaccatgt gccatcccgg gatcggaacg gtaacacgga
     6301 tgctctctcc gtctgcatgg atcgtgcagg caataccgga aagccccagg ctctcgatct
     6361 catcggcata gaccggcttc tgtccttcca caccgaaaaa tatcggtttg atgcctttta
     6421 cttcctcgat cgtatccagc ttgtcatcgt ccccatttaa aatgatgcag ccgtcttctt
     6481 tcagaaaatc aaagatttcc gtctttgctc ttaaaatacc gtcgcggtct tttaaaaact
     6541 ccagatgaca ctgtccgata ttggtgatca cgcagatatc cggtctggct actttggcaa
     6601 gacgatgcat ctctccaaaa tggctgatgc ccatctcaag gactgcgatc tgatgttcat
     6661 ctcgcagacg gaaaacggta agcggcagcc ccagctcgtt attgaaattg ccaagggtct
     6721 tcagcacatt gtatttctga gctaaaaccg atgcgatcac ttcttttgtg ctggtctttc
     6781 cgacacttcc ggtgataccg actactttga tatcgagctg ctgccggtag tactcggcga
     6841 tccctttgac tgcctccaga gaagatgcaa cctgaatgta cggtcctgcc gggttttcca
     6901 gctctttttc acttaaaaca gccagtgcac ctttctcaaa aacagatggg ataaagtcgt
     6961 gtccgtctac acgctctccc ggaattgcca caaacaggca accctttgct gcctgcctgc
     7021 tgtctgtggt aatactctct acaatactct gtttcatctc ttcgcttccg tggtaaacac
     7081 ctccgcaagc ctgagctatt ttttcaaggg acatattttt catgttaaaa atacctctct
     7141 gttcttttat tttgctgata cagcgatcag tttctcgcag aggtctgcaa aatccatgcc
     7201 aagtgcctgt gcttcctgcg gcagaaggct ggtcggtgtc atgcccggaa gtgtattggc
     7261 ttccagacag tacattttgc cgtccttgga catcataaag tccagtcttc cgtaattgtc
     7321 aagctgcagt gccttaaacc ctttcaccgc atatgcctgc atctgctctg tctgttcttt
     7381 cgtcagaacg gccggacagg tctcaacggt gcttccggct ttgtatttgt tcgtgtaatc
     7441 ataaaagcct tcaatcggtg cgatctcaat gatcggataa gctattccgt ccacgacacc
     7501 gactgaaaat tcacgacctt tgatatattc ttccacaacg atcacatctt cataagaaaa
     7561 tgcatctgca actgctgctt cgtattcttc ctgtgttttc ggaatcgtaa cgccgacgct
     7621 ggaacctccg caacacggtt tcacaacaca cggaagtgtc ataccatgtg cctcaagcga
     7681 aatatcctcg ccttttctca ggataaatcc tgccggtgtc ggaacttcat tctgccagaa
     7741 aagctgtttg gaaagtccct tatccatggc aatcgcactt cccagataac cacttcccgt
     7801 ataacggata ccgagcaggt caaaagctgc ctgtactttt ccgttctcac cgttctcgcc
     7861 gtgcagtgcc aggaacacaa catctgctgc ctgacacagt gcgatcacat tggtgccaaa
     7921 gaatgctttt ccttcctgaa tgctcttctc tacctgaccg tcaaaacttc tcgcataagc
     7981 ggctgcggca tcgacatcgt actcatcggt aaatgcctgc atcggatctg catcttctct
     8041 tccgaaatag acatccacta ggatagcctg atgacctctg cttcgaagtg ccttgcatac
     8101 ctgtgtaccg gatacgatcg atatctcacg ctcggtactg atacctcctg ctaaaacaac
     8161 aattttcata attttcctcc tcatgccgca gttctgcggt ctttttcttt ctatatagct
     8221 catcactgcc catctatggc tgtatacgac gtcggatcac tcagggtatc ggttgtggtg
     8281 cctgtcgttg tatcagccgc actgctctcc ggggtgactt ccactccctg ctcgttgatc
     8341 acacgggtct cagccggctg tgagattgcc acagagccgg tgccctgatt gatcgaagac
     8401 ttataaacaa cgatcggtgt tccgatctcg atattgtcaa aaataatacc tgcctgatcc
     8461 cacggtgtat tgacacatcc atgcgaacca tttccctgat aaagcgttcc gccaaagctt
     8521 gcacgccatt ttgcatcgtg gataccgaca ccgccgttga acggcatcca gtaatcaacc
     8581 ggtgtattgt agctcttgcc ggtcaggttg tcggctcccc gaaggatcgc ctttgcttcc
     8641 ttgtcaaaca ggcaataaag ccctagcgga gtctcgtaac cgtcttttgt cgcatcgccg
     8701 gtaacgaccg gagtatcaac cagacactgt ccgtctttgt agaaccacat acgctggttg
     8761 gtgatatcaa tctccacata ggtatcgccg atatcgtttt cttcccctct tgccatggca
     8821 ctttccagcc actgtggctg tcttgtcgca gattcgcctg atttaagtgt ctcatccagc
     8881 catgccgcct cggtctcctc atccatcttc cagccgtaat tcatgctggt tacggtcacg
     8941 gtctctccaa gtgaggtatg gaatgtctca tcggtaccga atgtatcgta cttgtctgcc
     9001 agctgtccga tccagtctgc gatatgattc cagtcaaaag aaacatttcc gctggaatct
     9061 actgtcatcc agtctttgaa tgtctggtaa tccagcacat cggaagcatc tccgatctca
     9121 tatgtaaccg tcatatccca gtatttctgc aaagatgcca gtttcttttt cagtttttta
     9181 ttgtcactgg tcttctttgg tttcagatag cagtcgctct cttccagatt tacttcggtc
     9241 ttgccgtccg tcactgcctg acagagcagc tcatagactt tatcttcatc cagctgattg
     9301 ccttccactt cctccacaat ctcaaatccg gaagacgttt catcaatata ggcatctttc
     9361 ggttcggtaa ctttatcgct gtcaaaagca tccagttttt ccatcgcttc tttgagcttt
     9421 gccttattgt aaaccgtcgg agtcgtcatc gtatactcct gcggatggaa gtatgcattg
     9481 atccataaaa aagagttctg tgaatatttc agctgctgtg ccgcaccgtc tgatacatac
     9541 tcaaactgaa tatccgcacc gtcaatctgt tcggtcttgt tgtctttttc atgtacccgc
     9601 agtacataat cctgcacctg gtttgcaacc agattttcta cttcttttac gttcttgccg
     9661 gacacatcaa tgccgtttac cgtcgtcccc ggaaagaaac ggtccatata gtaaacgcca
     9721 aatcccagat aaacagcaac catgatcagg acaacaatgc cggccacgat aagaggtgtt
     9781 ctggaatgtt tctttttcgg cagttccggt tttaccggct gctgctcata tcttcctggt
     9841 cttccctggc ttccttctct ccggcttctt tctgatcccg gtgttctctg cggctgttcc
     9901 agtccttcgg ctcttctgtg ccgttcttct cttgagcctt cccgtcttga accttctgtt
     9961 cttgaacggt ctcttcttct ttttcttttt ggtccatcgt ttaatgtgat ttccttcata
    10021 tacttttcct ttgcctctta aaaatactgt tatcatcccc ggtattttgc agcagagcag
    10081 acatttgcgt tccgctttca aaatacattc ctattttagc acgtatccaa aaaaagtaaa
    10141 cacctacctg tcaagaaaaa cagaccgaaa ttacaacatt ttaaatgcga tcagtacaat
    10201 ccccagaatc acaagtacca ctccggttgc acggtttaac gtttccggtt ttgccctgtt
    10261 tgcaaaacgt gccgcaatcc ttgcccacag gaacgtgaac aatacgcaca gcaccagaca
    10321 ggtgatatcc ggcatgccgc cgatcgcaaa atgagaagcc gcaccggtaa atgccgtaaa
    10381 cgccatgata aacacgctcg ttccgactgc ggtcttcaat tcatagccaa gcacacttgt
    10441 caggatcaga agcatcatca gtccaccgcc cgctccgaca aagccgcaga taaatccgat
    10501 cagcatgccg cagatcacag actggataaa acgcttcttt tttgagactg cctgcatggc
    10561 ttctttggtc gtcataaccg gttttacgat aaatttgaca ccaagcagca gtgtcatgac
    10621 catcgaaaat ccgcccatgg tcgtactcgg gagcagactt gcgatatagc tgcccagcag
    10681 cgtaaataaa agtaccgtga ccatcatcac gattccgttt gccacatcca ggtttttgtt
    10741 tttgccgtaa gtataagctg agaccgcact ggcgagcaca tcgctggcaa gtgcgatacc
    10801 gactgcctga tacggatcca tgccaagaaa agtgatcagc atcggactga tcacagctgc
    10861 cgcactcatg ccggcaaaac cggttccgat gccggctccc attccggcaa agaaacagac
    10921 taaaatcgta aaaatcatat tttgccctct tttctttttt tgtgttaaag cccattataa
    10981 aacatcttct ccacaaaaat caataccgca ggaaacgctt ctttcgcttc ctgcggtatt
    11041 ttatcttttc tttcaatatc ttttatggca gtccggaaaa aaacagatca gactgatccg
    11101 ttttgccgtg tcgttacggt aactgtgctg ccggtccatc tccaggcaaa acacatcctc
    11161 ttttccgacc aggtatgctt cctcgccgca ccggatcttt acttcgcctt ccagcacaga
    11221 aatgtatgct cttgtctttt tcccctgact gctgccggta tattctgccc ccggttccat
    11281 atcaatccgg taaatctttg ccacacagtt attttcataa ggaaaacagt tccatatttt
    11341 atattcgccc cttacttcct ttgtcggctc catttctttt atatgcacca gtctggaccc
    11401 tggagacaca tcctctatca actcctgaaa ttcaatgtga agtccgccga taatctttcc
    11461 gatcacacca agagaagggt ttgcaatccc tttttcgatc tgagccagca tacttttgct
    11521 caccccggtc tgttcagcca ttccctcaag actcatccct ttttccctgc ggatccgctt
    11581 taaattaact gcaacgttat aattcagatc gtccataact gctccctagt cctccgatat
    11641 actgatcaca ctcagttctt tccggtactt ttttttatgt tcttttttct ggaggtacat
    11701 ttcatgatct gcctgtttta taatatccct gacctgcagc tcctcatttc caccctctac
    11761 ttctacgata ccgcagctga aactcttcgg atattccttt gtctgcccgg atgaaaattc
    11821 tttctgtatt ttccttattt tccgctctgc catctcgtag ggacagttat acagaatcac
    11881 gcagaactca tctcctccaa tccttgcaaa cacatcttct tttcggatgt gtttttggat
    11941 cgtctcaaca aaaaacgtga tataccagtc cccttctgtg tgtccgtatt gatcattgat
    12001 atatttcaga tgatccagat cgcagtaaca gaaggtcaga ctgctctctt ctttcagcat
    12061 atcccctgcc ttctccagga aatagtgacg gtttccgatc tgtgtcagcc catctcggtt
    12121 cgcttccatc ctcagacgtt cttcccgcag cttctctttc gtcacatccc ggataatata
    12181 aagaaaggcc tgttccccct gccactgcat atatccggta atgatcctat aaaaatgatg
    12241 tcttgaatcc tctgcttccc atatccattc aaaagaatct ctgctctctt ccgaccggtg
    12301 tttctgtgcg aaccgaatac acatctggta aacttcattc cggtcaatac ttctgtcatt
    12361 cctcttttta cagtacagaa tcctcctgcc gtcggcactg gtcacgaaaa tctcctcttc
    12421 gctgcggtcg atcatctcta ccagaagctg attgtaagtt tctaccatcc cggcatgtat
    12481 tttttcccgc tctgcttcct gtttcagttt taattcccgc tccttcagct gctgcgtcat
    12541 cgtattgaat gcctctgaaa attccccgag ataagatact gtctgagaat agtcaccctt
    12601 tgccacctgt tttgcctgcc aggtcagatg atttagattt gcatggatat tttttaaatt
    12661 ttcgcacaaa aaattctctc gcggaggcgg ctgcaccgac agatttcctc tcgaaagggc
    12721 tgctgcgtac tgttttgttt ctgtaaacgt ctttgcaaaa tactgcagtc ccctgccaag
    12781 cttccgaaaa ggctcatcga gttgttccac atcaagcggc tgaatttttt tatcatacag
    12841 gatgcttctg aggtattcaa acaataattc gctgttttta tcctccatga ctatgctcct
    12901 atttttcgtc ttctatattt gcatgaaatc tgcacacgcg gtcaccggtt gcccagcagt
    12961 ctacctctac tgccgtatag aactttccag aatacatgct taaaataccg ccgataaatc
    13021 cttcgtcgta attgcatacc gtctcgccaa ggatcggaag tccggaacag tccgcatcct
    13081 ctgacacatt gagaatgatc tttccgctct cctcatctac cttttcgata cgcagcacac
    13141 cgatctttaa ttcctgcatt ttactctgaa gctcacttac aaactgatcc agcggctgat
    13201 ccagattcag cattttcttt gcaaaatatt ctcccgcctt gcgtccggca ttgcggaaaa
    13261 tctcaatctg cttttccttt ccaaactgtc cgatcagctc ctctttcagg gaatattcca
    13321 gcagacggta aaccatcacc ggaagctctt ctccgagatt gcctctgctg cccgcatcat
    13381 actgcagata atcctccagt gtaagaactt tccctcccgt cataaaaaca ttttcttcca
    13441 ttgtattctc ctctgcccgt gtccatttat tccttgactc atcattctat gtatttcagt
    13501 ataacatctc ttttttctca gttcaaccaa ttttccgata aaaagagcca taaccaactt
    13561 catttaaagt cagccatggc tctgttttct caaattttta tttatcttcc agaatcttat
    13621 cgatacttac cagttttacg ctcagtacaa agatttctgt tgccgttttc agctcttccg
    13681 gtgtcgggta agacggtgcg atacgaatat tggaatcctg cggatctttt ccatacggga
    13741 atgttgcacc ggcatcggtc ataacaacac ccgcttcttt tgctttggct acgattgctt
    13801 ttgcacagcc aggcagtgaa tcaaaggaaa tgaagtatcc tccgcgcggt tcaagccagc
    13861 ttccgatctc cagtccgccc agctcatttt tcagtccgct caatacagtt tcaaatttcg
    13921 gacgcaggat gtctgcatgc tttctcatat gcagcaccat gccgtggata tctttgaaga
    13981 atcttacgtg acgcagctgg ttcagcttgt catgaccgat ggtctggatg gtcatctgct
    14041 ttttaatatc tgcaatgttt gctttggaag taccgattgc agctacaccg gaacccggga
    14101 agcttacttt ggatgtagag cagaatttgt aaaccatgtc cggattacct tcttttttac
    14161 attccatcag gatctcaacc aggttgtcct gtttatcctc atataaatga tggataccat
    14221 atgcattgtc ccagtaaata cggaagtctt ctgctgccgg tttcagtctt gcgaaacgac
    14281 gtactgtctc atcagaatag gtgataccct gtgggttgga atatttcggc acacaccaga
    14341 tacctttgac tgccggatcg ttgttgacat attcttcnnn nnnnnnntat tcttcaacca
    14401 tatccatatc cggtccatct tcatgcatcg gaatgttgat catctcaata ccgaagtgct
    14461 ctgtaatctt gaagtgacgg tcatatccag gaaccgggca caggaatttt actttatcca
    14521 gtttgcacca cggagtgctg cccattacac catgagtcat ggaacgtgcc accgtatcgt
    14581 acataacgtt cagacttgag tttccataaa taataatatt gtccggagat acctctgtca
    14641 tatcagccag aagctctttt gcttctttaa taccatcaag tacgccatag tttctgcagt
    14701 caacgccttc ttcacatacc agatctgcat tgctgtccag tacatccatc attcccattg
    14761 acagattcag ctgtgcggta gaaggttttc ctctggacat atccagcttc aatcctttgg
    14821 cctttatctc tgcgaactgc tgttccaggt tctctttttc cttaagcagc tcttctctgc
    14881 tcaaatcctt gtacggtgtc atagtaatcc tcctcatctt tcatgtccat acgcaaaata
    14941 cacttgtttg ccctgcacgt atcctgctct atcagctatt ttattacgca ccttgggttt
    15001 cgtcaagctt ttttcgattt tagtgatgga acagacggat tcctgtaaat gccattgcca
    15061 tattatattt atcgcatgtc tcgatcacca ggtcatctct tacggatccg cccggctctg
    15121 caatatattt tacaccgctc ttatgagcac gctcgatgtt gtcgctgaac gggaagaatg
    15181 catcagaacc aagtgttacg tcctgcatct gatccagcca tgcacgtttc tcttcttttg
    15241 tgaatacttc cggtttttcg gtaaatactt tctgccatgc tccgtcagcc agtacatcca
    15301 tatactcgtc tccgatgtaa acgtcgatcg cattgtcacg gtctgcacgg cggatgcctt
    15361 cgatgaacgg caggttcaga actttcgggc actgacgcag ccaccagttg tcagccttct
    15421 gtccggcaag gcgtgtgcag tgtacacggg actgctgtcc ggcaccgata ccgatcgcct
    15481 gtccgtcttt tacatagcat acggagttgg actgtgtgta tttcagtgtg atcagagaaa
    15541 tcttcaggtc atttaatgca ttctgcggaa tgtctttgtt ttttgtcacg atgttggaga
    15601 gcagctcatc gtcaatcttc agatcctgac gtccctgctc aaaggtaaca ccaaatacct
    15661 gtttgtgctc cagttttgcc ggcacataat tttcgtcgat ctggattacg ttgtaatttc
    15721 cttttttctt ctgtgccagg atctccagtg cttcttcgct ataacccgga gcgatcacgc
    15781 cgtcggatac ttcacgtttg atcagcatgg cagtgtcttt gtcgcagaca tcggacagtg
    15841 caataaaatc tccgaaagaa gacatacggt ctgcacctct tgctcttgca taagcacatg
    15901 ccagcggtga aagatcaccc atgtcatcta cccagtagat ttttttcagg acatcagaca
    15961 tcggcagacc gattgctgca cctgccggtg aaacatgttt aaaagaagtc gctgccggca
    16021 gtccggttgc ctctttcaat tctcttacca gctgccagcc gttaaatgca tccaggaaat
    16081 tgatgtatcc aggtcttccc atcagcactt tgatcggaag gtcactgccg tctgccatat
    16141 agatcttaga tggtttctga ttcgggttac agccatattt cagttctaat tctttcattc
    16201 tgatttcctc ctgacgtttc ataatatacg ttaattcata agagttaatt cataagatgc
    16261 catacgaatt gttccgtact tcgtactctc acaattctta tctgttttta tttacgatct
    16321 ttgtctcata ttttccggtt gcgatgtcga tgtaacgtac aaacagggag actttgttat
    16381 cctcattcag actgttccac agggtttcgg taaatgcttc catatcatcc ggaattgcga
    16441 tcagtttcgg ttctccttcg aaacttggca gcggattgcc gtcgcactta taagtatgga
    16501 tgaaatgtcc ttcgcctgcc acacagtttt catatgcaaa cgtgtagcgg ttgcaggaat
    16561 ccggatttcc gttattgctc tttaaaatcg acattgcata gctgtatttt ccgttttcga
    16621 tgtgcatgat accggagata cgcggtgtat agttcggtgc atccggctca aactcacggc
    16681 ttcgaagaga ctgttcaaat gtaagctgac gatccatgcc ttcgtagatg gtatcggtct
    16741 gatctccgtt tgtaacgatc gtcttgtttc ccagcacacg taccggtgcg tagatgatca
    16801 ggctcgggtc aaccagcttg gaaggatcaa atgcctgtgt tcggatgcct tctccatcct
    16861 ccacaaagat acggttgcgg ctgttctcgc ttcttcccat gataaaataa gcggtcactg
    16921 cctttgtacc atccttggat cttccaatga tgatacctct tcccggataa gaattttcct
    16981 gaagttcttt ttcaatcgat aacatttcca tgtttgcatt ctccttttct tttcactctc
    17041 aacctgcttt aaagcactgt gccaatccac agaacatttc attttgccaa aaatccgaat
    17101 tcagaccaag gcaaaaaaaa tgtgccgcac actccaagca agatctgccc agacggtcga
    17161 cacttttacc cttatactcc ctgtggtcta ttccacttcc gtcagtcgta taattattta
    17221 aaccttacca tttccctgaa aaaaagtcaa ccttgatttt cggttttcag cctttttcca
    17281 tccagcactg cctcggtcag cacatcgccc cggtatttca gtttcagaga aaaaaacgga
    17341 tgctcttctt ttaaaagttt aaaaaagatt ttatcgcctt cccagaggtt cagcctttca
    17401 agctcacttt tctttaccca ctccagcgtt ccttcatcgc aggtgatcat atccccttca
    17461 aagccatccg ccgtgtaaag acacatatat tccgtttccc atgcatctga aagaaacgtg
    17521 accagtcccc ggaaacgcca ggaagtgagc gtaagccctg tttcttcctt tacttcacgg
    17581 agcagacatt cttccggcgt ctcgtccttc tcaaaatgcc cgccgacccc gatccacttg
    17641 tctttattga catccacctc ttttttgatg cggtgc
//
LOCUS       DAWDWH010000017        16989 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_1956_length_16989_cov_14.353490, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000017 DAWDWH010000000
VERSION     DAWDWH010000017.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 16989)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16989)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16989
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1956_length_16989_cov_14.353490"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    9850..9859
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12490..12499
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5189 a         3282 c         4536 g         3962 t
ORIGIN      
        1 gtcgtgaagc agcaatccgt gctcttcagg catgtggtct ggaagtaaca agcatccgcg
       61 acgtaacacc tgttccacac aatggttgtc gtccaccgaa acgtagaaga gtctaattag
      121 gaggttagcg aaatggcagt aaatagagtt cctgttctta aaagatgcag atccctgggt
      181 atggatccga tttacttagg atatgataaa aaatccaata gagagctgaa gagagcaaac
      241 cgtaaaatga gtgagtacgg tctgcagctt cgcgaaaaac agaaagcaaa attcatctat
      301 ggtgttctgg aaaaaccttt ccgtaactac tacaaaaaag ctgaaaaaat gccgggtatg
      361 acaggtgaaa acctgatggt tatgctggaa tccagactgg acagcgtagt attccgtctt
      421 ggttttgcaa gaacaagaag agaagcaaga cagatcgtag atcataaaca cgtaacagta
      481 aacggtaagc aggtaaacat tccatcttat ctggtaaaag ctggcgacgt tatcgaaatc
      541 aaagaaaaat gcaaatctat gcagagatat aaagatatcg tagaagtaac aggcggcaga
      601 ctggtaccgg aatggctgga ttgcgatatg gaagcactca aaggtacagt aaaagagctt
      661 ccagcaagag aagtgattga tgttcctgta aacgagatgc ttatcgtcga gctgtattct
      721 aagtaataaa tagcaccgta ctacggtgcg tagaacccct catacaccaa ataacccaac
      781 aaggagggac tatatgttcg attttaataa acctaaaatt gaaatagctg agatttcaga
      841 agataagaaa tacggaagat ttgttgttga gcctctggag agaggttatg gtacaacact
      901 cggcaattca cttagaagaa tcatgctctc atctttacct ggagcagcag taagccaggt
      961 aaagatcgaa ggtgtactcc atgaattcag ctcaattccg ggagtaaaag aagacgttac
     1021 agagatcatc atgaatatca agtcccttgc tattcagaac aacagcgaaa cagatgagcc
     1081 gaaagttgca tacatcgagt atgaaggcga aggcgttgtc accgctgctg atatcaaggc
     1141 agactccgat atcgtaatca tgaatccgga tcaggttatc gcaaccctga acggcggtac
     1201 aaactgcaaa ctgtatatgg aactgaccat caccaagggc agaggctata tcagtgcgga
     1261 taaaggtaaa acagatgata tgccgatcgg tgtgatcgca gtagattcta tctacacacc
     1321 ggttgaccgt gtaaactata aagttgagaa tacccgtgta ggtcaggtaa ccgattacga
     1381 taaactgacc atggatatct ggacaaacgg aacactggct ccggatgagg tagtcagcct
     1441 tgcagcaaag gttctgagtg ctcatctgaa tctgttcatc gatctgtctg agaatgcgaa
     1501 atcagcagag atcatgattg aaaaagaaag caatgagaaa gaaaaggttc tggagatgaa
     1561 catcgacgaa ctggaacttt ccgtacgttc ttacaactgc ctgaagagag caggtatcaa
     1621 tacagtagaa gagctttgca gcaagactcc ggaagatatg atgaaagttc gtaacctcgg
     1681 acgtaaatca cttgaagaag tacttggcaa gctgaaagag ctgggattac agctcaaccc
     1741 gagcgaagaa taaaattcaa cccgagttat atacagtgca ggcggatagc agccttggca
     1801 atcccggcac tggacattat ggacaagaca gcatctgtca agtaagcccg aagagcattg
     1861 gcagatgttc cacaaatgga ggaaaataaa atggcaggtt atagaaaact tagcagaaca
     1921 gcagaccaga gaaaagccct gatcagagga caggttacat ctctgctgta tcatggaaaa
     1981 atcgttacta cagaacagaa agctaaagaa atccgcaaag ttgcagaagg tctgattgct
     2041 ctggctgtta aagaaaaaga caacttcgaa actgttaccg ttactgctaa agtagcacgt
     2101 aaagacaaag acggtaagag agtaaaagaa gtcgtagacg gcaagaaagt aactgtatac
     2161 gatgaagtac agaaagaaat caaaaaagac aatccttcca gactgcacgc tcgtcgtcag
     2221 atgctgaaag ttctgtaccc ggtaacagaa gttcctgcag aagcagcagg aaagaagaaa
     2281 aacacaaaac aggtagacat ggtagacaaa ctgtttacag agatcgctcc gaaatacgaa
     2341 ggacgtaacg gtggttatac aagaatcgtt aagattggtc ttcgtaaagg cgataacgct
     2401 atgaaagttc tgcttgagct ggtataatcc ggacatcaat ttataaaacg ataacagagc
     2461 cgtttcgaat tcacaggatt cgaggcggct tttgtgctat caggatataa agctgccagg
     2521 cattttgccc ttaacatcat ttagaacata aaatggaaac gaatcgtatt tatcggaaag
     2581 gagacagaaa atcatgattt atataggaag ccatatttca tcatccaaag gctatgcggc
     2641 catgggaaga caggccttga agcttggggc ggatacgttt gcctttttca cacgcaatcc
     2701 gagaggcgga agtgcgaagg agatcaggca ggaagatgtg gataaatttt tgaaaattgc
     2761 agaagagggt ggatttggga aactggtggc acatgcacct tatactttaa atgcctgtgc
     2821 agccagagaa gaagtacgta cctttgccag agaggcattt gccgatgatc tgaggcgtat
     2881 ggaatacatt ccgggaaatt attataattt tcatccggga agccatgtcg gtcagggaac
     2941 cgaggaagga atccgcctga tttcggaaat gctcaacgaa aatctgaaaa aagaacagac
     3001 aacaaccgta cttctggaga caatggcggg aaaaggaaca gaagtcggaa gaacgtttga
     3061 agagctgcaa agtatcatgg aaaaggttca actttccgac aaactgggcg tgtgtctgga
     3121 tacctgccat gtatgggatg ccggttatga tatcgtaaat gatctcgatg gagtattaga
     3181 agaatttgac cgggtgatcg gtctgaagag attgaaagcg atccatatca atgacagctt
     3241 aaatgagaga ggtagccaca aagaccgcca tgcaaggatc ggagaaggta agatcggact
     3301 tccggcattt gagaggatcg tgcgacatcc acaccttcag gggctgccgt ttatcctgga
     3361 aacaccgaac gatgaggaag gctgggcaaa agaaattgag atgctccgaa gagcatccca
     3421 ggatcagtaa atatccgaca gtgtcagatt aacatttttc cggttccgga taatcaacgc
     3481 cgaatgtatc aacggtgatt gtgcggatta cctgaggatc gagcggagca tcgttccagt
     3541 cggtatctgt ctcagcaatt ttatttacaa catcaagacc atcggtcact ttaccaaagg
     3601 cagcatatgc accgtcgagg tgtggtgctg ctttgtgcat gatgaagaac tgagagccgg
     3661 cagaatccgg atgcatagca cgggccatgg acagaacacc tgcttcatgt ttcagcggat
     3721 tatggaagcc attctgggaa aattcgccgc ggatggtgta gccaggaccg ccggtaccat
     3781 ttcccagagg gcagccgccc tggatcatga aaccttcgat gactctgtgg aaagtcagac
     3841 cgtcataaaa gccttttttc accaggctga tgaagttgtt tacagaaact ggtgcgattt
     3901 ccggataaag ctcagctttg atcacatcgc cgttgtccat ggtaattgta ataactggat
     3961 tttgagccat aattgtatac ttcctttcta aaatgcggta taatgataaa atgctagggt
     4021 aaaagatcaa aatgtcgttc ttgcttgcaa gataagacat ttgagcttgt aacccgctaa
     4081 aacatgagga agcagcgatt tacaacgtaa atctgcgaat tccgaatgtt ttatagaatt
     4141 gtgggagtta cattgtaacg gagcaattcg tgggcatttt gtaggatata ccttcaacag
     4201 atatatccta gaaagtatat catgcaaatt tgcaggagac aaggagattt tcagagggga
     4261 cgctatctat gaaaacagtc gtatttcatt tacaggcgaa aacatgtgaa aaagacattt
     4321 caaagttaaa agaagaaatt ttatcttata atataagaat cgaagaaaaa gaactttccg
     4381 aggtaaaaga agaaaaagac ggtattctgg cgattacgga tcagttttcg gatgttgatt
     4441 atgcgaagga acacaacatc gcagttattt tctatgagac accgggaacg aaccgcagtg
     4501 tgtccggtgt ggacatggtc gtgcagggat ttgaagagct gaatgtagag tatctgcagc
     4561 ttatttatat gcgacatcac ggattgccgt ggatcattgc aaagacggat cggatctgca
     4621 tccgcgagag cgtggagggg gatcttacgg ctttccggaa gctctaccag gaagaaggga
     4681 tgctggatta tctgccgaat ccggagtttg aaggtgagga aggaagagaa aactttcgtc
     4741 attatatccg cagcatgtac cgtttttata attatgggat ctggacagtg ctcgagcgaa
     4801 aaagcggtgc aattatcgga agagtcggca ttgaaaacgg agaatatcag ggacagagcg
     4861 tactggaact tggctatctg atcgggaaag actggcggcg acagggattt gcaagagaag
     4921 ccgttttgat gacggaacgg tttgcagcgg aagttttgca ggcggattca cttcatgctt
     4981 ttattcaccc gcaaaatgaa tcatctattg catttattaa agaaattgga tacgaaaaac
     5041 tggaactgcc aggagaggac ggagttctgg tctggcaaaa aaatctgcaa aagggcagaa
     5101 tttagaaaac gtttagaaaa atttctccgg attgtttaga aagagcgatt aatatgaaat
     5161 agctctcgca gatgaatgag cttaaaaaca aaagaggtgg aaaaatgcaa atcgaatttc
     5221 agattttaga tgcactgcaa aagatacata cgccagttct ggatggcgtg atgtgtctga
     5281 tcacatcact tggaaatgcc gggatcatct ggattttact gaccattgcc ctgctgatta
     5341 atccaaagat cagaagaaca aaagacggaa cagcgtgcag acttttcggg gaatccggca
     5401 gacgaagcgg atgtattttg cttgcagcac tgactcttga cctgattctg tgcaacggta
     5461 ttttaaaaaa tctgtttcac agagtacggc cgtatgacat cagaacatcc atcgaacttc
     5521 tggtaaaaag accggtggat tattcgtttc cgtccggaca tacggcagct tcttttactg
     5581 cggtggtggc actttctttt gccggtgaga aaagggcatg gaaagcggca ctggttcttg
     5641 catgtctgat cgcattttcc agaatgtatc tgtatgtaca ctatccgact gatgtgctcg
     5701 gcggtgtgct tgtgggcatc ctggcaggat atggcggata ccgggtggtg aaagcactgg
     5761 aacagtataa aaaggaaaaa taatacagca ttttacgaaa gccccgttct gtggtatgat
     5821 aacagtcaga ttattacact acagaaaggg gcattttcaa tgggagatac ctcaaaatat
     5881 aaagaagaaa aacagatcgc actcgggatc ctggctcatg tggatgccgg aaagacgacg
     5941 ctctccgaat ccatgctgca caagggcggt attgtggaca atataggcag agtcgataaa
     6001 aagaatgcct ttctcgataa ttatgaactg gaaaaggagc ggggcatcac aatcttttcc
     6061 aagcaggcgg tgtttcggat cggaagtatg agagtaacgc ttctggatac accgggacat
     6121 attgattttt cggcggagat ggagcggacg cttcaggtgc ttgactatgc ggtgcttgtc
     6181 atcaacgggg cggacggtgt gcagggacat accaggacgc tctggagact cctggagcgt
     6241 tatcagatcc cggtgttttt gtttatcaac aaaatggatc agttcaaagg aaatgatgag
     6301 gaaaagcagg aagagcagga gcgtctcctt gccagtcttc agaaagaact gagtacttcc
     6361 tgccttgatt ttggcggaga atgggatgcg gcatttctgg agaacctggc tttgtgtgag
     6421 gaagaggtgc tggagcatta tctggaatcg ggagaagtag aagaggcaga tattgtaaaa
     6481 atggtcgcac agcggaaagt atttccgtgc tattttggtt ctgctctgaa agaggaaggt
     6541 gtggacaaat ttctgaaagg tgtggaaact tatacggcag aaagaaaata cggggataag
     6601 tttgcagcca gagttttcaa gattgcaaga gatgcacagg gaaaccggct gacctacctc
     6661 aaagtgaccg gcggcgtgct gaaggtcaag atgctgctca aaggtgaaaa tcgcaatgca
     6721 gaggagacgg agatctggga agaaaaggtc gaccagatcc gcatttattc cggagcacgt
     6781 tatgagatgg taaaggaggc aaaggcaggg atggtctgtg cggtcaccgg actggatcat
     6841 acgtttgcag gggaaggcct gggcctagaa gaagaaagca cgattccgct gctggagccg
     6901 gtgctttcct ataagatcga actgccgccg gaatgtgatg cccatcagat gcttcgcaaa
     6961 ctgcgtcagc tggaggagga agagccgcag ctgcacatcg tatgggaaga acagagttcg
     7021 gagattcatg caaagctgat gggagatgtg cagatcgaga ttctgcagag tctgatcaga
     7081 gagcggtttg gcgtggatgt ctcatttgga gagggcagca ttgtctataa agagacgatt
     7141 gcaggaccgg tggaaggcat cggacatttt gagccgctgc gtcactatgc ggaggtgcat
     7201 ttgcttctgg agcctctgga gcgtggaagc ggcgttcaat ttgatacgga ttgcagcgaa
     7261 gatctgctgg accggaactg gcagcggctg atccttacgc atctggagga aaaagagcat
     7321 atcggtgtgc tgaccggttc ggcgatcaca gatatcaaga ttacattgat tgcaggaaga
     7381 gcacatcaga aacacaccga gggcggtgat ttccgtcagg cgacgtatcg ggccgtgcgt
     7441 cagggactga agcgtgccag aagtattctg ctggagccgt gttatgagtt ctgtctggag
     7501 attccgatgg agaatgtcgg aagagcgatg acggatcttc agaatatggg ggcagatttt
     7561 gagcagccga tgatcgagga caagatggca ggcatcaaag gccgggctcc ggtttctaca
     7621 atgcgtggtt atcagaaaga agttatttcc tacacaagag gatttggaaa cctggtctgc
     7681 agttttggcg gttatatcga atgtcacaat gcagaagaag tgatcacggc atatgattat
     7741 gattcggagc atgatctgat gaatccgacc ggatcggtat tctgtgcaca cggagccgga
     7801 tttgtcgttc cgtgggatca ggttccagac tatatgcatc tggaagaacg caggatcgca
     7861 gatacgcagt cagcagacgg ggaacaggca tctgatgata aaaagagaag cgaaagtgga
     7921 caggcagacg gcagctggat cagttccgca gaatacaaaa agcgacaggc agattactgg
     7981 aggggcgaaa aagagctgga agagattttt gcccgcacgt acggagaagt taaaagaagg
     8041 cttccgtccg agcaggaata tcgcagagta catgtcacag cttccgagcc ggtctatcgt
     8101 cctgcaaaac cgaaaaaagc ggaaaaagaa taccttctgg tggatggtta caacattatt
     8161 ttcgcctggg aagagttaaa agagctgtca aagatcaaca ttgacagtgc gagagaccgc
     8221 ctgaaagata ttctgtgtaa ttatcagggg tatcgcaagt gtacgctgat cctggtattt
     8281 gatgcttacc gtgtgaaagg acaccgggag gagatcacaa aataccacaa tatccatgtg
     8341 gtctatacaa aagaggcaga aacagccgat atgtttatcg aaaagaccgt ccatgcgatc
     8401 ggcaggaaat atcaggttac agtagcaact tccgacggac tggaacaggt gatcatcatg
     8461 ggacagggcg gaaagaggct gtccgctgcc ggcctcaaag aagaagtcga tatggcggca
     8521 gctcagatga aagaacatct ggaggcggcg aagccaaaaa caaaaaccta tcttggggaa
     8581 tattttccgg aagattctgg aaaagcagag taaaattttt tgccggacag aagcccggaa
     8641 aaacgaatat catataaaac atagtataaa tgtcatatag tgaggtatcg aaaatgaaaa
     8701 tggcagaaga gcttcaaaga gagctccgca gtatcaaccg taaaagttat ccggcgtaca
     8761 aggggctgaa aggggcatat cagttcccgg attaccagct ttttatcgag catgtgcagg
     8821 gtgatccatt cgcagcaccg tccgcactgc ggatttttgt gccgcacagc aaggcaaaat
     8881 ttccggaaag atattattgg gataagtgca gcaaggttgc attgcaggat gctttgctcc
     8941 gacgttttgc ggaaatatcc gcaaaattct gttaccaggc aaaaggctcc ggtaagagcg
     9001 gtgtgatcca ggtcagtcac tgcggacagg aagttctgga gcgaactgcc tgtgagatca
     9061 caaaggaagg catccacatc cgcttttttg tcggttttcc ggcaaatgga cgaacgatca
     9121 attccgtcga actggaaaaa attctgtttg tgtatcttcc gaagtgcgtg gaaatgtctc
     9181 tgtatcacag aaaagttccg gaaagagaga ccgagcaggt gatctgttta aaagaggatc
     9241 agagggtaat aagggaagaa ttaaagaaaa gaggtctgat cgcatttgtg gcaaatggtt
     9301 cgattctgcc aagacagagc ggaaattctg atctgccgat gaaggatgcc gtcccgtttc
     9361 agtccccaaa gagcatggaa atcaccatac agcttcgaca tcgtggttcg atcacaggta
     9421 tggggatcag aaaagggatc acgctgatcg ccggcggcgg ttatcatgga aaatcgacgc
     9481 tgcttgaggc actcgaaaaa ggcgtttacg atcacatcgc cggagatgga agagaatttg
     9541 tgatcacgga tgatactgcc tggaaacttc gggcggagga tggcagaaag atcaaggatg
     9601 tcgatatttc gctgtttatc aatcatcttc caaatggaag aaataccaga agattttcta
     9661 ctctggatgc cagcggcagt acctcacagg cggcaaatat tatagaagcg atcgaggcga
     9721 aaagccaggt tctgctgatt gatgaagaca cttccgcaac gaattttatg gtgcgtgatg
     9781 agctgatgca gcgtgtgatc cagaaggaca aggagccgat cacgccgttt ctggagcggg
     9841 cgagagatcn nnnnnnnnna tgaaaaggca ggcatctcta cgatcttggt agtcggaagc
     9901 tgtggttcgt atttttatat cgcagatcag atcattcaga tggacaatta ctgtccggtg
     9961 gatattacag agaaaaccag aaaactgctg aaagaatata aaaaaccgga ctgtaaggca
    10021 gaaggctttg cactgccgtc tgaaaaaaga agtatatcct ttggaagctc tgtggtacga
    10081 aggaaaaatt accgcggaac cggtatggta gaagaacacg aaaaattaaa agtgatggga
    10141 agagaaagcc tgatgcttgg caaagaacag ctggatcttc gctatctgga gcagattgcg
    10201 gaccgggaac agacccagac actggggtat cttctcaaat atgcaaaaga gcagtacagc
    10261 ggcaaaacaa cagatcttac ggcactgatg gaatccctga tccggaaact ggaaaaagaa
    10321 gggatcggaa gtgtgagcgg gcagaaagag attccggccg ggatggcaat gccacgaaga
    10381 caggagattt atgcgtgctt taatcgcttc tgaccctggc atctttgaat cgttacaaaa
    10441 atattacaaa tatttacaat tttacatatt tgtatcaaaa ttgttatgta ccatgtatgt
    10501 gtcgggggat ctggaagtgc agtacatgaa aagattgccc acgacattct tgaaagcatt
    10561 tgtcaggaga tgacaggcgg agaaaatgtg aaatgctgaa gagaaagcgt gggtgttttc
    10621 tgttcagttc gatgataaag gaggtacaaa acgtatgaaa cgcagaaaat atttacttgc
    10681 ttgcctgctt gcaggtgtta ccattgccat gccgacaggg cagctcagtt ttgcagcagg
    10741 ggcacagaac gatatcagcc tgatggcaga gtccgaaagc cagagcgaga cacagtctga
    10801 aagccagagt gaatcggaaa cacagagcga gtcacagtcc gaaagtcaga gtgagtccga
    10861 aagccagagc gagacacagt ctgaaagcca gagtgaatcg gagacggaga cacaggaccc
    10921 gaatgttccg gaaggataca aaggcattta caaggtcagt gacctgagtc tgttatcttc
    10981 caagccggac ggaaaatata tgctgatggc ggatctggat ctgaatagtg cagaacaggc
    11041 aagtatttct gcatttcgcg gtacactgga tggaaacaac catacgatca aaggactgaa
    11101 gcaggcactg ttccaggtgc ttgaagaggg aacggtgagc aacctgaatt tatcgggtgt
    11161 gcagatcagc ggcagcggcg ataccggtgc acttgcaaat gtcatcggaa ccggcaaaaa
    11221 aagtgcagtt acgatccaga atatcacgat caccggaagt gttacaggta ccgggaatac
    11281 cggagcactg gcgggtgttt tgaaggggac agatgtcgtg atctcgagga tcgctaatag
    11341 tgcgactgta gccggaacgg caaatgccgg cggcctgatc ggacttgtac aggcaacaga
    11401 tgaccaggaa ggggcggcat ctgtgaagct gacagagagc tataacatcg gagaggtcag
    11461 tgcggtaaca gcaggaggac tgatcggtgc agcagagatc ccaaatctgg cagcggcaag
    11521 aaacattgag atcgcaaact gctacaacgc aggcgtattg gaaaaagggg ccaatatcgt
    11581 agcgaagatc acagcaggga acggtcaggt tgtgatcaat aaatgtgtcg gtattcagtt
    11641 ttattattct gtggcatccg gcatggtcgg cacgtccaca atggcagcag gcagcacaca
    11701 gacatccggc tgcagcattt caaacagcta ctattacagc atgaatccgg tctatgcatt
    11761 tgaaacagga aactgcatta ctggaacagc aacggcactc aatgatgtgc agatcaagac
    11821 acagagctat ttcaaagatt ttgatttcga tggcatctgg gctttgaatg cagcagaaaa
    11881 cggcggttat ccgtatctga aacagaccac catgctgccg gtcagctatg tggcaccgac
    11941 tgccggaacg gttctgatag acagcaaaac gggcggcaag tatcttgtaa gcaccagaag
    12001 caaagcagtt tcctacgcag gcgtgctgga tgaggagatc acttccgtga gcatcccatc
    12061 tcaggtggag atcaacggca taacgtataa tgtaacggcg atcgcagaag gggcactgag
    12121 aggaaacaca aagatcacct atgtatccgt tccgtacagt gtgacggaag ttggaaaata
    12181 tgcatttaag ggatgcacat ctcttgtaaa agtaagcacc ctcaaatatg tgaagaatat
    12241 ttatcaggaa gcatttgacg gctgtacaaa tctgaccacg atcggcgtaa ccaattatcg
    12301 gatcacactt ccgtctgttg aaatgatcgg tgtgcgtgca ttccgcagag tgccaaaggt
    12361 gcacagagtt tatatttcct caaagaatct gactacaatc aagaaaggtg cgttccgtga
    12421 gtgtaccggc atgaccaagt ttaacgtagt ttcaacgaaa ctgaacagtc tgcagaaata
    12481 tgcactgtan nnnnnnnnna gcaagctgaa aacgatcatc ttcaaaagcg agcacctgac
    12541 aaagagcaat gtgggaagca agacatttag cggaatcaaa tcaacggcaa cctttacggt
    12601 tccggcaggt tccgttcaga attataaaga tttatttaag agcaagggag cggggagtaa
    12661 atttaaggtt aaaagtttgt aaaaaagatt gagaagcgtt aagggtattt acaaaaaact
    12721 catataaaat aaaaaaactt ctgagatccg gtatggattt cagaagtttt ttaatactta
    12781 gcttataacc ttttcaggct tttgctgtgt cacggaagat cacatggctg taatttttca
    12841 gggcactttg cagaaccagt ccgatcacac cgcaggaaag tacttcaccg atgccgactg
    12901 tcagcatcat aaacgggatc ggcagattga caccgtatcc gtagtaaagg acgaacggta
    12961 cgatcagggt gttggcaatg atcggcggca gcggagctaa gaatctgctc tttttacgca
    13021 gggcataagt tccgagggct ccaagcagtg tggcaatgct gccgaaaata atatcagcag
    13081 ggattgcacc gccgaacagg ttggcaataa tacatccgac aaacagtccc ggaatcgcag
    13141 ccggtgtgaa gaatggcagg acggtcagtg cttcagaaat acgaacctgt acttcaccga
    13201 aactgaacgg tgcaaagatg atcgtcagca cgacatagat cgctgcgatc acagcagcct
    13261 gggttaaaaa ggttaccttg ttagactttt tcatgatatt tttctccttt agttttgttt
    13321 tatgagtgga aggtcacgaa cactcagcac attttacggc ggtgaccgcc ggcaagcccg
    13381 ataagacctt gtttttaagg gctttgcaac ggctcaaagt ataacacagt gccggaaaga
    13441 ttgcaacaga aagctggaaa atccatgctg tgccagaatc tggaagcgta tttcgacact
    13501 tccctgaaag tgtataaaaa tttggcattt ttggaggaat gtataccaga tttggtagac
    13561 aaaaagggaa aagtaagata aactaaaatt tgtggtaaat gctcttttgc atttacaaag
    13621 aggtaaaaaa gaaatcatgt atcagaaaag gcgaagatgg agtatgagga ggaaaaatga
    13681 agtttgaaaa agaagtgatg cagaatcttg ctatggtcac acagcttggg atttctatgc
    13741 tggctccgat cattctctgc gtattcgcag gccactggct ggatgagcat ttcgggtttc
    13801 acagcgtgat cgtgctgctt ctctttggta tcgcagcagg tgtacgcaac tgttatctga
    13861 tcttaaaaca gcttattccg aaaaaaggag aaaagaaaca tggaagataa ggtattgaaa
    13921 agtctgattt ccggtatcct attatgtgga atactgtttc aggcagtctt tgtgtggttt
    13981 tctgatgcaa aggtcttttt ttcactgggg ctgtggatcg gagtcggcgt tgccgttttt
    14041 atggcatggc acatcaggta cagtttagaa gatgcacttg atatgtcaga gggcgatgct
    14101 ccgaaacata tgtaccgtat gtatggcatc cgtatggcag tcgtgctggt tgtatttttt
    14161 attactgcct attttgagat cggcagtatg atcggcgttc tgcttggagt gatgactctg
    14221 aaagtcggag catatatgca gcctttgctt cagaaaataa aaaagaaaag aaggtgaatg
    14281 agtgagagca ggttttttgc tggcggcaag tggtgacgtg gattttatga tccatggatt
    14341 gttcaagtac aggttattcg gacaggaatt ctggatcacg accacgcatg tcagcatact
    14401 cgtggtgatg ctggtactgt ttgtgtttgc acttgcggca aaccggacga tgcgtcacgc
    14461 acaggaagtg ccgggatttt tccagaacat cgtcgagatg atcgtggaaa tgctggatgg
    14521 catggtagag agcagcatgg gaagttatgc gaagaaatat cgcaactaca tcggatcgat
    14581 ctttatcttt atttttgtca gcaatatctc aggtctgttt gggcttcggc ctccgacagc
    14641 ggactatggc gtaacacttc cactgggaat tatcacattt tgtatcatac agtacaacaa
    14701 tgtgaagtat aacaaatttg gtgcatttac cgatctgttt aagccaatcc cgatcctctt
    14761 cccgatcaac ctgatcggag agatcgcagt gccgttttct ctgtcactgc gtctgttcgg
    14821 aaatgtgctt tccggaacgg ttatgatgtc actgatctac tcactgcttt ccaagatcgc
    14881 agtgggctgg ccgggatttc ttcatattta ctttgatctt ttctccggtg caatacagac
    14941 ctatgtgttc tgtatgctga caatggtatt tacttcacag aaaatgccgc aggaaaatta
    15001 aaagatgtca aaaccccgga agcggatccg ttcgcagcag gtgggcggca tcaacgggca
    15061 acatcaagaa atcatatttt aatatttagg aggtacatct catggatgga attacaggta
    15121 aggcactggt tttagcatgc tctgcaattg gtgcaggtct tgcaatgatc gcaggtattg
    15181 gtcctggtgt aggtcagggt atcgcagccg gatatggtgc aagtgcagta ggaagaaacc
    15241 cgggagcaaa aggtgatatt atgtcaacca tgcttttagg tcaggcagtt gccgagacaa
    15301 caggtcttta tggtcttgct atcgcattta tcctgctgtt cgcaaacccg ctgatcggca
    15361 agctgtaaaa agggggcaaa acgttggaac gactgtttaa tctggatcct cagctgattc
    15421 acgacgccgt tctgctggca cttgcagtat ttgtcatgtt tacgtttctg tcctatctgc
    15481 tgtttaatcc ggcaagagat ttcctgaaaa agagacagga taagatcaaa aatgatctgg
    15541 ataccgccgc agcagataaa gcagatgcac gtaaactcaa agaagattac aatgcgaaaa
    15601 ttcagaatat caatgcagag gcagaagaaa ttctggcaca ggcaagacag aaagctctgg
    15661 ataacgaagc gaagatcatt gccgaagcaa aagaagaagc tgcaagaatt ctttcaagag
    15721 caaatgccca ggttgaactg gagcgtaaag ctgctgcaga tgatatgaag aaagaaatga
    15781 tcgcacttgc atctttgatg gcagaaaagg cagtagccgg tgcggttgat gcaaaagttc
    15841 aggatacgat ggtagagagt acactgaaag aaatagggga ggacacatgg cgaaagtagc
    15901 ggcgaaggta tacggtgacg cgctgttcga gctggcggtg gaagagaacc gctgtgacca
    15961 gtttctggaa gagatttgtg ccgtagagaa cgcacttgac cagaatcccc aggcagagac
    16021 gctgctctgc catccgggta tcggcagcga ggaaaagcag gattttattg aaaaatgttt
    16081 caagggaaac gtctcagacg atatgacagg atttttacag attcttgtga agaagggacg
    16141 cttttccgag agaaatgcca tatttgcata ttttacagct aaagtgaaag aatacaaaaa
    16201 aattggtgtg gctctggtga catctccatt tgagcttacc gcagaacaga agactaagat
    16261 cgaaaagaga cttctggata ctacggacta caaaaagatg gagatcacat accgcgagga
    16321 taagacgctg atcggcggtc tggtgatccg gatcggagac cgtgtcattg acaccagtat
    16381 aaagagcagg ctggaagagc tgaggctgca gctcatggga atttccctcg aagcctgaga
    16441 gaagaaaaca gcatgaaaga aggtgcaaag accttatgaa tttgaaacct gaggaaataa
    16501 gctcagtgat aaaagaagag atcaaaagat attcttccca tatgtccgtt gcagaagtgg
    16561 gcacggttat tcaggttgca gatggtatcg cacgtatcca cggccttgag aaggcgatgc
    16621 agggtgagct tctggagttt ccgcatggtg tgcggggcat ggtgctcaac ctggaggagg
    16681 ataacgtagg tgccgtactg ctcggtgatg caacgaatat cgaagagggc gatatcgtaa
    16741 agactaccgg caccgttgtg gaagttccgg taggcgatga gatgatcggc agggttgtca
    16801 atgcccttgg acagccgatc gatggcaaag gtccgatctc aagcaggaaa ttccgtaaga
    16861 tcgaaagagt agcttccgga gttatcgacc gaaagagcgt agataccccg cttcagacag
    16921 gtatcaaggc aattgactcc atggtgccga tcggaagagg acagcgtgag ctgatcatcg
    16981 gagaccgtc
//
LOCUS       DAWDWH010000018        16798 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_1984_length_16798_cov_16.971033, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000018 DAWDWH010000000
VERSION     DAWDWH010000018.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 16798)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16798)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16798
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1984_length_16798_cov_16.971033"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    12440..12449
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5388 a         2700 c         4332 g         4368 t
ORIGIN      
        1 agcaggatta ttgtcggaga ggtgcggggc gatgaagtcg ttgatatgct tcaggcgatg
       61 aatacgggta tggacggatc actcagtacc ggtcacggga acagtccgtc ggatatgctg
      121 gtccggctgg agacaatggt tctgatgggg cttgagatgc cattgtccgc ggtacgcaga
      181 cagattgcat ccggaatcga tatactgatc catctgggga gactgcggga taagagcagg
      241 cgtgtcctgg agattcgtga ggtcaccggt tatgattatg aatctggaga aatagtaact
      301 tcggtgctgt atgaatttct ggaagatgga aagcaaaaag aagggcgtgt cgtgggaaaa
      361 ttacataaaa aagaggagct gttccatgtg cagaaactga tacgtgcagg aattgtgtgc
      421 taggttcacg gtcgtttgac ctgaacgtaa ataagcagag aaagcagatt gaaaaggggt
      481 ttgtatggat tataaagttt atcagataac aaaaaaggaa atgtttaggt atgcggcggt
      541 gtatattctg gcagatgttc tgatcagtct tttattttac cgttctgtgc ttgcagccgt
      601 ggtttttctg ccgggcatct gtttcttttt cagagaagtc agaagagaac tggcaaagaa
      661 aagaagaaaa cagctggagc ggggatttct ggtcgggatg cggtgtgttt ctaccgccct
      721 gacggcggga tattcggttg aaaatgcatt tacgcaggca tatgaagaac ttcagaaatt
      781 gtacagtgaa aaagaaccgg ttttacagga atttcgcagg attcgtgtgg gaattttgct
      841 gaaccggacg atggaggagc tgctttctga tctggcacaa cgcagcggag tggaagatat
      901 tgagattttt tctgaggtat ttgagacagc gagaagaacc ggaggcgatc tgatcgccat
      961 catacgcagc acgactgcct ctgtatcgca gaaggaagaa accagacagg aaattgagat
     1021 ctgtctttca gcaaagaaaa tggagcagaa cattatgagt ctgattccat gtctgctgat
     1081 tgcctatgtg ggactggctt caccgggatt tttagatgtg atgtatcaga atccggcggg
     1141 aattgcagtg atgagtatct gccttggtgt ttatgtgttt gcttttttga tgggaaggaa
     1201 gattgtagcg attgagatat gaagaaaaga tgatgcagcc gttttataaa atcggaaaat
     1261 ggatcgcatc aaaaatggta aagaaagaag atgtaaaata tcagaaactg gaacagacac
     1321 tgcagtttgt cgaaaattcg gcaaaggaaa aagacgcttt tgaatttacc gcaagaagaa
     1381 ttgcaaaagt aatgatgatt ctattctggg gcacaggact ttgttttctg gcagccgttt
     1441 ttacgggaga agcaaaagaa attacatttc ttgcaagacc ttcttatggg cagggaagca
     1501 tggaaacgga tctggaagta caggtagaag gggaaggaga agcacagaaa atcccgattt
     1561 tgctgcagga aagacagtac agtgaccagc agatcgatga gttttttcag caggcgatcg
     1621 aagatctgga aagcgtgatt ctcggggaaa atatatcgat gcaggaagta agaagttccc
     1681 tggaatttcc ttcttctgtt ttagatggca aagtgaaagc agactggttt acagatgatc
     1741 tgggtcttat tgatgaaaaa ggaaacctgg tcggggaacc ggcagagggt ggtacgcagg
     1801 tacagattac agtaaatttt tcctgtcagg acagagagat ggactaccgt ttttctgtgt
     1861 gtgtttttcc gccggtgcgt tcggaggaag agaaacagca aaaacagatt caggaatttt
     1921 tacagagtga agaacagaaa agggtgcagg aagaacagct gaggcttccg ggtgaaatag
     1981 agggcaaaaa gctggaatgg aaaaaagaaa aagaatcgcc tgtgcagatg attgcggttc
     2041 tggtggttat cacagcattg tgtatctgga tgctggaaga agaaagatta caggcagcag
     2101 tcaagaaaaa gcaaacggct cttaccatgg aatatgcttc ctttctctat aaattaatgg
     2161 cattgctgcg ggcagggctt acgatccggg gagcttttga aaggctggca cagagcagac
     2221 aggtccgtga gcactatata gacgaagaaa tacggcgttg ctgcaacgaa atgaaaagtg
     2281 gggttccgga agcacaggca tatgaaaatt ttggcagaag atgtcagctt ccggagtaca
     2341 tcaaaatggg aacgatactg gcacagaatc tgagaaaagg ttctgatgga ctggcggatc
     2401 ttctggaggc agaagctttg cggggaatgg aagagcggca tcagcttgca cgaaaacttg
     2461 gagaacaggc gggaacgaaa ctcttatttc caatgatgct gatgtttggt gtggtgttga
     2521 ttattctgat ggcaccggca ctgatgtcat tttcatttta gaattactgt gcatcttctg
     2581 ggaaaggagg tgaaaatata tgacacatgt gcaggcattt ctgaaagatg agcagggaat
     2641 tacaacggta gagattatcc tgattctcgt tgtactgatc agtctggtca tcatcttcaa
     2701 aaaacagctt accaatctgg taaacaaaat cttcagcaaa atcacaagtc agagcaacag
     2761 tatctgatcc attcagtatc tgaaaaatcc aacatgaaaa catttagaga acatttaaaa
     2821 acgcaagaat gcacagaaat ttcgccagat gaatatcagc aaaacaatta ctcaccactt
     2881 gcagaagtgg gagtcttctc gactgagata aaaatcatgt gtctggtctc atgtgcagca
     2941 taaacttggg aggggagtaa atgtatagaa ataaagggac aattacggtg ttcctcagtt
     3001 tgatcagtat gctgtttttg tcccttttct gtacgatggc agaatcggta agggtgcagg
     3061 cagcaagatt tcaggcagcg gcagcttttg atatgggatt gttttccatt tttggagaat
     3121 atgacagagt acttctggaa gagtatgatc tgtggttctt ggatgggagc agtgaagagc
     3181 aaaaaatcag agaagtgctg gagacaaagc tggaaaaata tattcagcca aatatcaatc
     3241 ccagatataa tctggcagca ggaagaagct gggatgtatt tcctacagag cttgatagat
     3301 gtacggtgga taagtatgct cttgcgacgg atcacggtgg acaagtgttt tacagtcagg
     3361 ctgtggaaaa tcagaaagaa ctgtttgtcg gggaagcggc atcagcttta aagaaaaata
     3421 taaaacaaat ggaagagggg catcaaaaag gagaagaata tcaaaatgaa gaacagaaag
     3481 cagatgagga gtatcaaagg gcacaggctg ctcagatgga agaaaaaaaa cgacaggaat
     3541 ccgaaaaaga aaatgccgaa aagcaggaag agtcggatac ggatataaag gaaacagagg
     3601 ctgttcaggc aaaagtggag aatccgcttg atcagataaa gaaaatcaaa agtatgggga
     3661 ttttaggtct tgtcctgaaa gatggtacag tggtttccga taagacaata gaaagaaaag
     3721 atctgccgtc tgagcgtaac ctgcaaaaag gttcacttct tgtcgatgaa atttctgcgg
     3781 atccagtttc agaagccatt tttttgaatt atcttcagaa acatttccag tgtgcagttg
     3841 atgaaggagg aaagcatcaa aagggaagca aagaacacag tctttcttat gaactggaat
     3901 atatcatcgg cggacagaaa agtgacagag aaaatttaaa gaaaacagtc aatcgcattc
     3961 tgctgatccg tgaaggtatg aactatgcta cgctggcaaa aagtgccaag atgcgagggg
     4021 aagcgatggc actggcagct gcgatcacag gggcagcagc actgccggaa tttgcaggag
     4081 cattgcagaa actgctgatg cttgcctggg catatgggga aagtctgctt gatgtgagaa
     4141 cgttgcttgc aaaaggaaag gttccgatgc ttaaagcaga aacagagtgg aaacttcctt
     4201 tagaaaagct tgggatgctg gcggaggttt tgaaagaatg tgatgccggc ggcggaaacg
     4261 ggcagtctta tgaagattat ctttctggtt tattgatact tggaaaaaag caacagcgta
     4321 atttgagagt gcttgatctg atcgagtgta accggagaat ggaaaagggc ggagaaacct
     4381 ttcgggtgga tgcacttgtg gcacaggcag aggcaagtgc agactttgaa ctggctcctg
     4441 tttttctgag ggttccggca gtctggatgc atgtcagaaa tcaggggact gcttatacaa
     4501 taaaaggaca atatggctat gggcagccgt agaaaagaag gtatttaagg atgttctttc
     4561 ggagaatttc ccgtatgaaa cgcagagaga aagaacataa aaatttgaaa atgaaaggag
     4621 gcgtgctctc tgtctggttc aaaattaaaa atattaaatc atttaacaga catccccctg
     4681 gctgttatca aaaaatccgg gagaacatcc ttaagtgcca gtcttacagt ggaaactgca
     4741 cttgtgcttc cgatttttct gtatctgatg cttggtgtac tcttttttgt tcagtttatg
     4801 gaaacagccg gagctacgga acagaaaatc catgaccagg caaaacagat ggcagtctat
     4861 gcttatgatt cacggaagat aacagatcag gactttcatc ttggatattc tactttttca
     4921 ggaaaagaag atgaaattga tctggtggcg gcgtatcgaa tcagcagtca gatgccggta
     4981 ttttcttttg gaaaaccaca gattatccag agaggatttg taagggcatg gagcggaagg
     5041 gattttcgca aagaactgca gaaagaagga aataaagaaa gcaaagagaa ggtttttgtg
     5101 accgcacatg gtaccgttta ccataaaagt ctgaagtgta ctcacttgcg gctgtctgtg
     5161 gagcagacaa cggttacaca ggcagaagcc agaagaaatc gctatggagc gaaatattat
     5221 gcctgttcat gctgtaaatc cggagttggt gcagcagttt atattacaga taccggcaat
     5281 cgttatcatg ccagactgtc ttgcagcgga ttaaagagaa cgatccggga gataccgctg
     5341 tcagaggcag ggaaacgtaa agcctgttca aaatgtggag ggtgatcata aaaatgaaga
     5401 atcagattgt ttatattctg ccgggaatat ttctgctgat aaacgcctgg tatgatataa
     5461 gaaaacgaaa aatattattg cacagtatat ggatatttac agttttgtgg attctttttc
     5521 tgatgtttgt ggataaagag aaaatctggc aggcatttgg tggaatactt ccgggaatgt
     5581 ttctgcttgc cagtgcttat gcgacaaggc aggccattgg ctatggagat ggactggcag
     5641 tttgtgtgtg cggtctgtat cttggaatgg gacagacctt tgcgatgttg ttttatgcat
     5701 tgctgttctg ttccgtggtt tcagctgtta tgctggtgtg taaaaagagg acttacaaga
     5761 caggcgtgcc atttgtgccg tatctgatag cgggatattt atgcacgttt ttgtaatgac
     5821 tgggacggag atgaaaatta tgcaaaaaat caaaagagac ttgaatggaa gttttacggt
     5881 ggaggctgca ctgcttatga cagtgctgat tccgttgctt accgccctta tttatcttgg
     5941 attttatcag catgataagg tctggatcgc aaataagacg agaacagagg cgatggaagc
     6001 agccctggac caagacagaa aaaaccagac ggattggagc ggagtgcttg cctgcggcaa
     6061 cgtttccggt ggtgtaaaac gtggaaaaaa taaagtggca gcgacaggaa aggcaacgtt
     6121 cagaattccg ggatttgtgg cacgcttctt tatgggcggg caaatggaaa taaacagtaa
     6181 agcggaagta tccgttaaaa atgcaaaaaa acagattcaa aaatatcgaa atttgcagcg
     6241 ggcaggggag ggataacttt atggatgttt cttatcgcag agataaagat cataattata
     6301 tgatactgga tgctccgggg gaactgaccg gcgaggaata tcaggtacgg atgctggttg
     6361 gaaatcatat tcctcattta ttaaaatgca atcgccggat gatggacgga aaagcggtct
     6421 ttttttatga ggttaccgga atgcagccga tgttccgtgt gtttgaaaaa aggcagatca
     6481 atcaggaaga aatggttgaa cttttgcagg atatcaaaga ggcactggaa aacacccaga
     6541 gatatctttt agatggaaat caactgctct ttgaaccgga atttctcttt tttcatacgc
     6601 agacacggga atggaatctg tgctatcttc cgacgtatga tggaaatatg acagagagtt
     6661 ttcgaaagct ctcagaatac ctgttaaaaa gactggatca cagtgacgaa caggcagtga
     6721 tgcttggata cgaagtatac agcaaagcat ccgaagaaaa ttatcggata tcggaagtgc
     6781 tgcagatgat ttatcaggtt ggacggaaaa attgtgctca ggaggaagga ttgccggaag
     6841 acccggaaca gtcagagaaa atgaaaattt caaaagagtc ggaagtttct gaacaggaaa
     6901 actttgaaga tctttgttcc gaggattcgt ttttcgagca ggaagaatca gaatacacga
     6961 ttccgaaaaa tattgagaac atcaagactg cgaagcagag agaaaaaggc atttctaaaa
     7021 atgagaatga tatgcagaga agaacggata aatatagagg tcaggcggaa caaaaaaaac
     7081 aaattcagaa taaaaaaaca accggcttaa aaaaatcaat gaaacagaaa aaaacagccg
     7141 ggcgtaaaaa agcagccaaa tataaaaaat atgcaggcta tgtagtaatt tttattctat
     7201 tggcagcagt ggcagccgta ctggtgaaat ttgaggtatt aagtaccgtt caggtcggag
     7261 ggatgctgtt tctggcggct ggcattttta tttatgcggc agggattcaa aaggaaaatt
     7321 tccaaaaagg tgaacggaag aaatcacagg tatcggatga ttttgatgag gagatgggaa
     7381 cctgtatttt gcagcaggga aaaaatatta agatagcagt tctggtcagt ctgaatccgg
     7441 aggagtatgt taatattctg ctgtatcgtg aagaaacccg gataggaaaa gaaggacggc
     7501 aggcggatgt ctgtatcgat agggatgtga tcagccgtgt tcatgcaaaa attctgagaa
     7561 gagaggaaga atattttctg atggatctgg attcgacaaa tggaacgtat ataaacggaa
     7621 aaagacttgg tggggatggc atggaaaaac tacagcaggg tgaccgcatt tcttttgcag
     7681 atctggaata catttttcaa ttacaataaa gatagcacat gatatgtcag aaatggcgga
     7741 tgagaattgt aaaactaaaa aaaacataaa aaatacacct ttgtctgtta cagaaagact
     7801 tcctgtagta tactgaaatc ttagaatgtt ggggaaaact taaggcataa aatcacgctt
     7861 gtgcaaaaat atcgcagtca ttcttaagtg tggtttcagg cgttaaaact ggcatcatat
     7921 tatacagaag gaggtgaaaa agtagcgaat ggatgaaatc agggattttt tgagatctcg
     7981 ttcggttgca aatcttacga ttgtcatcat taatgttgca gtatttctga tcctgtcctg
     8041 ttttggtgat acagaaaatg cagattttat ggcggcacat ggagcaagtt acactcctta
     8101 tatcgtgcag gatggaaaat attatcttct gattaccagc atgtttttac attttggatt
     8161 gagccatctg tttaacaata tggtagtgct gatcttcatg ggagatatac tggaaaagaa
     8221 acttggaaaa ataagatatc ttctgattta ttttggagga ggtattgcag gaaactgcct
     8281 gtccgtttat atggacataa aaagagcgga atttccggtt tcagcgggtg catccggtgc
     8341 aatttttgct gtgatgggtg caatcctgtg gctggtaata aaaaacaaag gaaagctgga
     8401 tgatatttcc ggccgaaaat ttgtgcttat gatcgtgctt tctgttttcc agggttatac
     8461 aagcatggga gttgacaaca gtgcacatat cggtggtctg atcatgggat ttctgttatg
     8521 tatgattctc agcatagggg taaaacaatc cgaagatgac tacctgacat atgattaaac
     8581 agtgtgtctg atttatccag attatcggaa gtgtttttac tgccggaagt atcttccgga
     8641 aaaagaaaag caatactgcc gtggtgtgcc tcgataatac ctctggcgat cggaagtcca
     8701 agaccggtac cgtttggttt tgtagtgaca aacggtttga aaaattcagt ctgctgttct
     8761 ttggagatgg aagctccgtc atttatgata tcgatctgaa ggcagtcctg tttttcaaca
     8821 acagaaaaag atacagattg tgcggcagat tcaaaggagt tgcggagcag attggtaaga
     8881 gcctgattta atttcagggg atcaaccgaa agtgtcggca aattttccgg aacagaaatc
     8941 tgataggaaa tcggggaatc cggaaaaagc agatatacag actcggataa ttttaagagg
     9001 aacggagaca tttccgtaac actgaactga cagcaaaagg tgttgttgta gcaggaaaga
     9061 tcatcgagaa gtctgcgaag aaaaagcatt tcttcggtga tatcactcca gagttcgttc
     9121 tgctttaatt ctggatgatc tttttcaagt aactggagat agctgttgat cagagtgacc
     9181 ggattgcgta cttcatgtga tacttttgaa agagcaaagc ggtagtcttc aagttgtttt
     9241 tcgagagaag atttatcagc agccatagtg tattcctccg atttctgatt ttttattgat
     9301 ttcaggattt tgaccctggt atattaaaaa gtgtatcatt tgggcaacaa aaaagcaaac
     9361 agaaataaac gacataattc gacaataatt gtataaaaac gaataaaagt aaagtttttg
     9421 gcaggaataa acaaaaaagt gcaaaaaatt aggaaaacat attgaaatta aaaacgaaaa
     9481 gcattaatat atagcataac agataaagtg gataaatttg ggtagcactt tattggaaca
     9541 acaaaaaagg aaggggaaat gacttatgag agatgataag tgtatttttt gtaaaattgc
     9601 aaatggtgaa attccatcag ctacgttgta tgaagatgaa gattttcgtg tgattctgga
     9661 tttgggaccg gcgacgaaag gacacgcttt gattctgccg aagaatcatt tcgccaatct
     9721 ttttgaaata ccggaagata tggatgccaa ggcatttatt cttgccaaaa agattgcaaa
     9781 aaaaatgaaa gatgtttttg gatgtgacgg cgtaaacatt gtgcagaata acggtgttgc
     9841 ggcaggtcag acggtattcc attttcatat tcatctgatc ccgagatatg aaggagatca
     9901 tgcaggcgta acttggaaac caggaactct cacagatgag caaagagaag agattctgca
     9961 gaaatggaag taacagggga tgtcgaaata tcggtaggga gaagagtagg atgaacgaaa
    10021 agaaattcac agaagtcttt aataaatata acaggcttgt gcgaaaaatg gtgatcagca
    10081 gatctggtaa cgatatgctg gcagaggaga tttgtcaaca ggtattctta caatattttg
    10141 agcagatgaa caacatctca gaagagttga ttcagccatg gcttttgctg acaacaagaa
    10201 atatggtcta tgattatctg agaaagatgc aagtccgcaa agatacacac agcatcaatg
    10261 gaatcgaaga tttcatggtg atccatgagg ataatacaga gcgtgtcata accagattat
    10321 ctcacaatat gctgacggaa cgtattcttg aagagttata tgacaagaaa aaagaatggt
    10381 attatgtgat catggacatc tgtattttgc agatgagtta tgaagaagca gcgaagcacc
    10441 taaacattga agtacaggtg cttcgtgcaa gactctacag agccagaaga tatattcgaa
    10501 agaaatatgg agaagaatat caggaacttt aattttttcc ggaagtaact tcttcaatca
    10561 gagaggtgat ttttgaaata gcatcgctct gaccgctttt ttccatagca gctacatagt
    10621 cagcacgttt ttcgtatagt ttatctatga ttggcagtat aatagttggt gccagatttt
    10681 cttcggtaag aacttcactg aatccctgat tttcaaagga ttctgcgttg aggatctggt
    10741 cgccgcggct ggcatttgcg gaaagcggaa tcagcagatt tggttttctg agtgccagca
    10801 actcacaaat agcgtttgca cctgctctgg agatgcagac atcggcaagg gcaaacagat
    10861 cagcaagttc ttttttgacg tattcgaact gaatataacc ttccatatct gtttttgtcg
    10921 aatctaattt tcctttaccg caaagatgga tgacctgata ttttttcaaa agttcaggca
    10981 gaataccgcg gacggcatca ttgacggcaa cagatccctg gcttccgccg ataacgagga
    11041 taaccggttt gtttgcggca aatccgcaga ggttcagtgc ggcaagttta ttgccatgca
    11101 gaagctcttc acgaatcggt gagccggtca ggactgcttt tccttccgga atgtaaccca
    11161 tggtttcagg gaagttgcag catacttttg ttgcagcaga aagagaaatc ttgtttgcaa
    11221 gtcccggtgt catatcggat tcatggataa taaccgggat atggagatgt ttggctgcaa
    11281 gaacaacagg aacagctaca aaaccgccct tggaaaaaac aacatccggt ttcagctttt
    11341 tcaggatttt acgtgcctca ccatagcctt ttaatacacg gaacggatca gagaagtttt
    11401 ttggatcaaa atagcgtctc agctttccgg aagcaattcc atagtaaggt acgtttacat
    11461 cttcaatcag ctttttttcg attccttcat aggagccgat atagtgaata tcataaccaa
    11521 gttcccgcag ttttgggagc agagcaatgt taggtgttac atgaccggcg gtaccaccgc
    11581 cagttaagac gatacgtttc atgtttatcc tccctgtttt ggtattttgc ctttaaagca
    11641 gacagaaggg gctgttttaa agggaatata ctaagtttaa atattatgat gtgattgtca
    11701 aaaaatactg taaaaccaca cttgtgcagg cactttgtgg tttcaggatt ttgactctgg
    11761 tatcatctta tactgttatt aaaaaaagtc aagaagggac tagaggattt atgaaaaaga
    11821 atgaggggaa aatcaggaat attcattcac gtcagaaccg ggagaacctg aaggaagctg
    11881 aatttgattc aaaactttat tcaacgatgc gaagcaattt tgcaaaagag ggaaagaaaa
    11941 ttgcaggcga gcttgaaaat gatgaaaaac tggattatgt aggtgatgct ccggatctgt
    12001 taaataaaat tgtcggacag cttagagaag aaggcaaatg ggatgataac gcctatagac
    12061 aggaatgtaa aaaagagctt tacgatctgt tgccgcagga agatcagatt gcactgagaa
    12121 gaggacggaa actggcaaag atcgaagaaa aatggaagaa gaacagaaaa aaaattatgc
    12181 agcgggcagc aatcattgtg ctggtcggag gagtttttgc ggctggatta ggagcggatg
    12241 cgagcagaga gcgtattatg aatgtatggg gaagagttac aagtacaggg cagaggttag
    12301 ttatagataa aacagatgat acgattttga agggcaagga agatgaagat agacacaaga
    12361 taaaagaaga attaggaatt aacataccca aactcatata taagccagaa ggtatgaaat
    12421 atgtaagtta tgatcttgan nnnnnnnnnc ttcaaacagc aaatatgaat tatttaactg
    12481 agaataaaaa gtattactat atgtatgtaa gtaaaaaagc aactgattca caggaagtga
    12541 ttgagattga tggaaaaatt atgaatcagc atttgctgag tgtggaatat ttagataaag
    12601 gtgtaaaggt ggaacagatt caagaagata gtggaagaag tttatataaa gcagattttt
    12661 actataacga tgtctattat ttgttcatgg gtgaaatgga aaaagagcag tttgatgaaa
    12721 taattgaaaa aattatgttt taacgcgtca cttttttcct tctgaaacgt ttattatagt
    12781 gtaatgtaaa aaacagagat aaaggaggag aaaaacatga ggatgaagaa agtgttcaaa
    12841 gcgatgctgg cagcgacggc ctgtgcgtgt atgctgacta tgccgcttaa tatccatgcg
    12901 gcggagccga cagatggtct ggtggtcgat ggatcgctgc ttactcataa tgacagtaca
    12961 gagacaacgg aactgtttga atggcgattt gatagtccag agtcagatgt agcaccatta
    13021 ggtacttatt atgctaaggg tcatgctgga atttcaaaag taagttcaaa atctgcttac
    13081 ataacagcga gaacagattg ctatgaaaat tgtgatactg tggtagcaga agtgaattta
    13141 cagcgtctgg aaggaaatac ttgggcatat gtaacaacaa gaagtaaaac cggaaataat
    13201 acagggagtg tcaaggtaag cgatacggtt cgtgtaaaat caggctatta ttatcgtgtg
    13261 gtatctttcc acagtgctac caaaaacgga aaaagagagg ttggttcagc taccagtgag
    13321 agtctttata tcggctagcc tgctttgaag catgagctgt actgagattc attgtaaaac
    13381 gtataaattc acatcgtatg taaatgacaa tgacataaat cttaatattc ctatcgcgat
    13441 tgtgaatgtc tccatctaca gtgaatcaac gtacaggctg acgtgtgaat ggcgtgaaca
    13501 gttacgtgaa ttttattttt cattgcgaag agagtaaatg tatttatgat gtgtgagtgt
    13561 aacgattcgg tgcaggtcga agcatcatcc tactgctgaa actgtgaaaa gcacggaatg
    13621 gttatgtgtg agtttaagca aaaatatatt agagcgtgaa aaattgttca ggtatgaaca
    13681 aaactctgaa aaaacaagcc gccccgggat gcgtgaatgt ccattacatt acctttcatg
    13741 gcagaacggt tttcgccaaa gatatggcga gtgatttaaa acagaaacta caaaaaacaa
    13801 aaacgaaaga attacaaata tgcaaaaaca aaaactgtgc ataccatgcg tggggcttcc
    13861 ctcctttgca gttgtgccgg gcttgttctg caaaggaagt atggttgcat catgcttttg
    13921 attgcaaggc aaggggagag agcgtatgca aacgaaatat tttaaaaata tgttccagaa
    13981 tatttcacag ggagttatgt attccacagg agttgcagat tcagagacaa agaacagctg
    14041 ggtagcctgt tatatctgtg caggagaaaa ggaggagtct ctggagttta aggcagagaa
    14101 agcttttgcc ggatgtaagg atgaggtaag gcttgtggct gcgggagatg ggagcgaggc
    14161 aaccattcat cagcttatgg aatttgtgaa aaagaatcgt gtggagcagg tgattcttcc
    14221 tggaaaccat gaaaaggtgg caaaagaact gcaaagtgcc ggtgtaaaac gtgtggtgga
    14281 gatgaagccg ggcagctctt tatatgatga aaaggacttc tggcagttta agatacattg
    14341 ttatggaaca gatgagggtt gtttccttgt aatgtttgcc ggagataaag gaatagactg
    14401 gaaaacaatg gactgtctga tgagtgtcag aatttttgat aaagctaaat gcggcaatcc
    14461 tcaaattgat atggaaaatc ttgtgtgttg tgccagatgt gggctgtata atgattttga
    14521 tgtgtgcaaa ggccataacc agaacacagg aaaaggatat acagcaggtg cgatgcttct
    14581 tggtaatata tcacttaaaa agtattcaga agcaataaaa agggatttct cagaggtgag
    14641 agatcagatt cgttacattt ccataacagg aaatatgaaa caggcagatg gggaaatgag
    14701 tacgtggatt ggggaatcac gtacggaatt aaatcactat tatattttac catccggatg
    14761 tgcagacaca ggagatttta taacaaaaat cctttccgag tcgggacgca atcgcgtata
    14821 cctgacagga gagaaatgtg gtgtttgcgg atcgggattt tttatttccc gaaagctgga
    14881 ttaaaaatta cagtactaaa aaatcaggat atgtgataaa ataaaaacag gaaaacagaa
    14941 cggaacagat tatcattaaa acagggagaa ggtgttttta tgaggattac atttttggga
    15001 gccacgcatg aagtgactgg aagctgtact tatctggaag cctgcggaaa acatattttg
    15061 attgactgtg gaatggagca gggaccggat gaatttgaaa atcaggagat accggttgcg
    15121 gcaagtgaca ttgacatggt acttttgacg catgctcata tcgaccattc cggtaaactt
    15181 ccgcttttgt accagagggg atttcgggga cagatttttg cgacgagagc aactgcggat
    15241 ctgtgtgata ttatgctgcg agacagtgca cacatacaga tgtttgaggc ggaatggaag
    15301 aaccgaaaag gacagcgtgc cggcagggca gaagttgtgc cgctgtatga tatcaacgat
    15361 gcacagggag cgatcaactg tcttgttgga tgcgattatg acaaaaaaat caaccttgca
    15421 gatggaatct gcattcggtt tgtggatgca gggcatctgc ttggctcttc ttccatcgag
    15481 atctggatca aagagaacaa tgtggagaaa aagattgtat tttccgggga tatcggcaat
    15541 ctgaatcagc cattgatccg tgatccgcag tatgtcaaag atgcggatta tgttgtgatg
    15601 gagtctactt acggtgaccg cagccatggt gagtttccag attatgtgac ggagctggca
    15661 aggatcatcc agaagacgtt tgatcgggga ggaaatgtag tcataccatc ttttgcagtc
    15721 ggacgtacgc aggaactgct gtatttcatc cgcaaaataa aagaagataa gatgatccac
    15781 ggccatgatc attttgatgt ttacgtggac agtccgctgg cgattgaagc gacccgcatt
    15841 tttatgaagc acatgtatga ggatttcgat gaagaggcaa caaagcttgt tcaggcgggg
    15901 atcaatccga tcggttttga aggactgaaa acagcgatca caagtgacga ttccaggatg
    15961 atcaatttcg atgagagacc aaaagttatt atttctgcga gcggtatgtg tgaggcaggc
    16021 agaatccgac atcatctgaa acataatctc tggagaaaag aaagcacgat cctttttgtc
    16081 ggttatcagg caaagggaac actggggcgt attatattag aaggggcaaa atccgtaaat
    16141 ctgtttggag aggaaataga gatacaggcg gagatccgaa ggcttgccgg tatcagcgga
    16201 cacgctgaca ataatgggct gcttaaatgg atcggaagct ttgaaaagaa gccggagaag
    16261 gtttttgtga ttcatggaga ggacagcgtg tgccaggttt tcacagatcg tctgagaaac
    16321 gaaatgcatc tggatgcagg agcaccttac agcggtacgg tctttaatct tgcgacgggt
    16381 gcgtatgaga aagaatgtga accggttcat gtggttaaga ccgagaagag tctgaataag
    16441 gcaatgaagg caaatactcc gtttgcaaga cttctggcag caggtcagag actgcttacg
    16501 gttatccgcc ataacgaagg cggagcaaat aaggatctgg cgagatttac aagtcagatc
    16561 aattccctct gcgataagtg ggacagataa tgagatgatg ttttcataca ggtcaacgca
    16621 tttgtcactg aaaccaaaag aacatgattt gaacgcagag aagcggattg cgaatgtccg
    16681 ctttactgta taaaaatcaa aaagctcagg ctgcggatga taccgtgtgc ctgggctttt
    16741 gtaaggtgcg cctagcggca cacgtttcta acgggttaaa gtcccgaatc cgcccggt
//
LOCUS       DAWDWH010000019        16609 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_2012_length_16609_cov_17.157907, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000019 DAWDWH010000000
VERSION     DAWDWH010000019.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 16609)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16609)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16609
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2012_length_16609_cov_17.157907"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    16218..16227
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4012 a         4213 c         3242 g         5132 t
ORIGIN      
        1 agctcccctt gtttttctat tttcttttta tttaaaaatc cgaaaccatt ttttatggct
       61 ctccttctct cttgtcgctg ccaccatctg ccggttatgc tgaaaggagc gaatggtttc
      121 atccagattt cggtaacgtt cttcctctct tttttcctgg atcttcatca ggatctccat
      181 ttcttttaaa acactgtctg caatccgatt ttccagctcc acattatttt cctgaagagc
      241 acgtttcatg atatcaccga caatgtcctg aaatttttcc aggttgtcca gtgaatctac
      301 aaacggaagt atggtctgtg gtttcgggac ttcctgtgtg ctgtctttta aattcgcatc
      361 cgaatctgtt ttttctgtat gttctgcttt ttcagttttt gttgctgtgt ctttcattac
      421 tgcatttttc tcttctgtga tttccgttat ttcttctgct tttttctgta tttccggaat
      481 cagcaccttg atcgctttga gctgaaatcc tttgtcccgc agttctttga ctttcttcat
      541 gatatagatc gttttatcgg tatagtagcg atgtccaagt tccgttctgc ccactttgat
      601 gtccagctct tcttcccagc ttcgaagtac atgtgcctcc acccctgcct gtctggcggc
      661 atcggaaatc atgtatctga tctcgctcat cctctacctc ccaatattgt aaccgttcaa
      721 tactttcgca attacaatgc aaaaccagtt aaaacaactg cttccatttg tattgaatca
      781 ttacttatgt ttttggacat tgtccctttt tgatgcctgc gagtttgacc ctggcatatt
      841 tacatgagaa tgccaacgaa tttgtaccct ggcatattta catgagaatg ccaacgaatt
      901 gctccgttac aaagtaactc tcgcaattct ataaacattc ggaatccgca tatttactgc
      961 gtaaatagct acttcctcat gtttagcggg tcccaagttc aaatgtctta tcttgcaagc
     1021 aagaacgaca ctttgacctt tgtaccctgg cattctcata tgattgtaac atatttcttt
     1081 ctcaaaaacg caaaaaagat atcgcaaaaa gcgacacctt tttcatcccg gaaactccag
     1141 gcgttatttt tttaagtttg caaatttggt gaggtttggc agccatgcaa gctctaccgt
     1201 accgatcgga ccattacgct gtttggctat gatgacttct gcaatgttct ttttctcgga
     1261 atctttattg taataatcat ctcgataaag gaacattaca acgtcggcat cctgctcgat
     1321 cgcaccggac tctcgcaggt cggaaagcat cggtctgtga tccggtctct gctctaccgc
     1381 acggctcagc tgagaaagtg caagcaccgg aacctgaagc tctctggcaa tctcttttaa
     1441 ggatctcgaa atatcggaaa tctcctgctg tctggaatct gcacgtttgc ttcctgtcat
     1501 cagctgcagg tagtcgatca tgatgacatc cagacctttt tctgctttgt atttgcggca
     1561 tttggagcga agctccgcaa cggaaatacc aggcgtgtca tcgatgatca ggttggaacg
     1621 tccaacgatc ccggctcctt cgatcagttt cgcccagtca tcatcgctca gatttccggt
     1681 acgaagctgc tgtgaatcta cctgtgattc gagtgcgaac agtcggttaa ccagctgttc
     1741 ttttgacatt tccaaactga agatcgctgc cgtataattt ttcttaaatg ccatatactg
     1801 ggctatgttc agtacaaatg cggtctttcc catggaagga cgagctgcga tcagtactag
     1861 gtcggaaggc tgcataccgg ttgtcttgta atccagatca ataaatccgg tcgggattcc
     1921 ggtcacgctt cctttgtttc tcgatgccgc ttcaatccgg tcgagtgccc gcaacacgac
     1981 atctttgatc ggcacaaagt ctccagaatt tcgtttttga acaatatcaa aaatccgctt
     2041 ttctgctgat tccagaatat cttccagttt ttctttttcg ctgtagcagt catttgcgat
     2101 ctgttcattg actttgatca gttttcgcag aactgccttt tcatgtacaa tctgtgcata
     2161 ggcatctgca tttgtagagg tcagtgcaga agtcagcaga tctttgacaa actccagact
     2221 gctgatctca ggcggtacgt ctttttcacg cagaagattc tgcagcgtga tcagatctac
     2281 cggtttgccg tcattataaa gctctacgat cgcatcaaaa acaatgccgt actgatgctg
     2341 atagaaatcg tctccgctta tgatctcgga ggctttgatg atggcttcct gatccatgag
     2401 catagcaccg attacagact gctctgcctc aacactgtgg ggcaataccc gcttgatcag
     2461 tgcttcttcc atttttatgc cccctgttaa ataccttcta ctacgacttt cagctctgct
     2521 gttaccttcg gatgcagctt aatcgcaacg cttgttgtgc cgactgcctt gatcggatct
     2581 ttcatctgga ttttcttctt atcgatatca tatccaagct gtgcttttgc agcttctgca
     2641 atttctttgc tggaaacaga accgaatact tttccgcctt cgcctacttt tacagaaact
     2701 tttacctgtg cctttgccag ttcttctgca aatgctcttg cgtctgccag ctgttctttt
     2761 gcaacttttt catcgtgtgc ttttttcagt ttcagatcat tgatgttctt tgcacttgct
     2821 tccagtgcaa gttttttcgg gaacaacata tttcttgcat atccatcgct tacatttacc
     2881 atttcacctt tttttccaag tgcttttaca tcttctacaa gaataacttt catttaaatc
     2941 gctccttctt ctatcatcgt tcttatcgta cttttcagct tatcttttgc ttcttcaagt
     3001 gtcactccgt ccagctgtgc cccggcgata ttcagatgac cgcctccgcc gagacgctcc
     3061 ataatgatct gcacattgac ctcatcgatg gctcgtgcac tgatataaat acgctctttg
     3121 tattctgtca atacaaaaga agccttgata ccgacaatgt tcagcagttc atttgcggcc
     3181 tgagcaccga ctacagtcgg gctttccaga tcctcgctcg gacagcagct gatcgcataa
     3241 tactgctcaa atacttctgc gtggcgaacc gcttctgctc tcgccttata ggaactcatg
     3301 tcattccgga acatcttgcg gacacgcgtc acatctgctc cacatctgcg taagaatgct
     3361 gccgcttcaa aggtgcggac ccctgttttc gccataaaat tgttggtatc aataataata
     3421 cctgcataaa tacagtctgc ttcttttcct ttcagacgga tatcatctgc aaagtactgc
     3481 aaaatctctg caatcatctc acatgcggat gatgcatacg gctcgatata ggacagaacc
     3541 gcatttttga tcacttcgct gctctggcgg tgatggtcaa gcacaacgat cgtcctggtc
     3601 tgattcagga tctcagaaca ctctgtcatg cttggacggt tcgtgtcaac aaccaccaca
     3661 acggtgtcgt gatccacgat ctttctcgcc tgctcatgtg tcacaaacat atcttccgga
     3721 tattctttgt catttaggaa cagattcacc cactgacgga tcgatccatt gatatctccg
     3781 agcacaatat gggctttttt gtcaagaatc cttgccgcac gccatacgcc gacggctgct
     3841 ccgaaagaat cgatatccgt gatcttgtgt cccatgatga ccacatcttc gtggcttccg
     3901 ataaattcgc gaagtgcatg tgcctttact cttgctttta cacgcgtgct cttttccatc
     3961 tgctgggatt tgccgccgta gtaagaaatt ttatcgttat cttttacgac tgcctgatca
     4021 ccgccgcgtc caagagcaag ctcaatggca attcttgaaa actcataatt ctgtgcataa
     4081 gtactgccgt tcactccgat accgatactt aaagtgacct tcatttcgtt tccgatgttg
     4141 accgtcttta cctcttccag aagggaaaaa cggtttgctt ccagttcttc cagtgactta
     4201 tatttcagaa ctacaaaata cttgtccttc tcaagttttt ttgtgatacc gtccagattc
     4261 gccacatatt tgctgatctt acggtcgatc agggcaagca gaagggaacg tcttacctcc
     4321 tccacgctct caagcacttc atcgtaattg tccaagtaga tcagtccggc aaccattttc
     4381 tgttcctgat tctgacggat gtaataattc agctccgtat tgtcaaacag atacagtgcg
     4441 attagataat tcccttcctg cggaatatcc acaatcgata cttcctcgtt gagatctccc
     4501 attgaaagct tctgcagatg tgcctgataa tcgttttcat tataaaaaat ctgcacatct
     4561 gtttcttcct ttttgcacgg aagtgcagac gcatcaatct ccggaaacag gtttgaaata
     4621 tttttccgat acctggaatc ctttcctgtc acatcacaga atgcatcatt catccagagt
     4681 actcttccat ccgtatcgag taccgcatac ggaatggaaa aatttttcag catccctttc
     4741 tggatctggg aatactggga tgcaaacgca atcatctcac ggagaacaaa cggacggtaa
     4801 cgcagcatca gaacagacat cagaacccaa taagcggcag taaaacaaaa taccagaaat
     4861 cctgctttca catcgatcgc aaaaaccagg atatccagca tcacaagtaa gaccgccacg
     4921 actgccggcc actgcatata ggattttaat tttccgctgt gccttacctt ctttttctta
     4981 ttcattgcat cttctccaaa ccctcagtta ctatcaaagc ctatcatacc attttttccg
     5041 gaaatagaaa aggttttttt acgcttccag ccattccctg accacctctc tgataacaag
     5101 cagcatcagc ggaccaaaca aaactcccgt cggtccgaag atgcagatcc ccacatatac
     5161 tacaactgcg ataacaatgg gatatatgcc cagcttttcc cctaaaagcc gtggctccag
     5221 gaaatcccgc gtgctgttgg taatgagaaa gaaagtcagg taagctgcgg catgaaagaa
     5281 gtccccgcga aacagttcca gtgccgccca cggaagcagg atcgtccctg tcccgataaa
     5341 cggaagtgca tccagaaaac caatcaggat ccctaccagc agggcatagg aattctgcaa
     5401 aacccacaga cctgccacgc actctgccat cactgccgcc atgatcagaa gctgtgcccg
     5461 aagccacaga cccgccatct gccacagacg ttccccaatc cgtaccagat gacgaaacgt
     5521 gctgtatttc tgcaatttct gacagatttc atcataatct ttgatgatca gcaggacagc
     5581 cacaaaaatc acaaagagca cactgaacca ttctgcggca acgactgcat accgcacgga
     5641 atatcgaaaa acctgcgtga tattgactgc ctgtattttt tccgacattt tttcaatgtt
     5701 ctggtagacg atctctctga tcgcctcact tttcatcccg aatttctgat tgagcagcac
     5761 acagcatccg tcgatcatcg tttccatctt tgcctcgtac tgatcaatat gcagaaaaat
     5821 gctgcggatc tgggcaaaca gccgcacacc gaggaaccac atgcatacgc ctgttattcc
     5881 aaggatcagg ctcatcatgc agaacaaaag actccctttt cctgctttcc gaaaacacgg
     5941 atatttttgc agacggatgg cagccggatt taaaatcctt acaatcaaaa aagccaccag
     6001 aaatggggcc acatagacga atatatattt aaaaatgata tataccgcca ttatgattcc
     6061 cacaattccg attgcttttc tgatttttgt attcatcttc gccgcacctt ccttatcctc
     6121 ttttatcgga tttagtatgt acgacataat ctttttcatt ctgttatcct gtttttaccg
     6181 gatattgcta ttctttgcgg aatttttcaa acgctcctcc ttccggaaga acggtgaaat
     6241 ccatctgttt tccggttttc ggatgctgga aacttaagct gtaagcacag agtgccggag
     6301 tttctttttc gcgtgttcct gtcgtttttg cggatttttc gtcgtatttt ttatcacccg
     6361 caatcggcat gcccgctccg gaaagctgta cacggatctg atgatgccgg ccggtcagaa
     6421 gttcgatatc taccaggctg agatcctctg tttcttccag gatcgcataa cgcagtttgc
     6481 tttcttttgc acctttcgtt ccctttggca caatataaga agtgtttgtc ctgcggtctc
     6541 tctgaagata atccgtcagt tcctgatatt ctgctgctgt ttctgtttct ttgtatacat
     6601 cggcatttct acgcacaact gcacaatatc gtttcacgac ctgaccgctg cccttccggt
     6661 ctgcaatctg actgcttaaa ttcgctgctg cctgttttgt cttggcaaat acaatcacgc
     6721 cctggaccgg ctgatctaag cgatgcacaa ctccaagata tggctccccc ggtttcccgg
     6781 actcttccag aagatacagt ttcagaatgc tgaccatatc tttgactctg gaatttgcac
     6841 tctgaaccgg aactcctgcc ggtttgcgac atacgagaat atagggatcc tcatacagaa
     6901 tcccgatatt ccctaccatt ttgatacatt gtttctgatt gctgttttcc gttttcattt
     6961 ctccacacat aattctgtct gcctcagtca gattacagat attttttcag tgcatccacc
     7021 ttgtccagtt tttcccatgg cagatccagg tcgttgcgtc caaagtgtcc gtaagctgct
     7081 gtctgtttgt agatcggtct tctcagatcc agcattttga tgatgccggc cggtcttaaa
     7141 tcaaagttct cacgaacgat ttctgtgatc ttttcgtcag aaagtttgcc tgttccgaag
     7201 gtatctacca taacagaagt cggctgtgcc acaccgatcg cataagagag ctggatctcg
     7261 catttgtctg ccagtcctgc tgctacgata ttctttgctg cgtaacgtgc tgcataagct
     7321 gcagaacggt ctacttttgt gcagtctttt ccggagaatg ctccgccgcc gtgacgtgca
     7381 tatccgccgt aggtgtctac gataatctta cgtccggtca gtccgctgtc tccgtgaggt
     7441 ccgccgatca cgaaacgtcc ggtcggattt acaaagaatt tggtgttctc atctaccagt
     7501 tcctgtggca ggatcgcatc aaatacatgt tttttgatgt cttcgtggat ctgttcctgt
     7561 gttacgtccg gatcatgctg cgtagacaga acaactgcat ccagtcttgc cggttttccg
     7621 ttttcatcat attctacggt aacctgagtt tttccgtccg gtcttaagta aggcagtgtt
     7681 ccatctttgc ggatcttggt cagctggcgt gccagtttgt gtgccagtgc gatcggataa
     7741 ggcatgtatt cttcggtttc gtttgttgca aaaccgaaca tcataccctg gtcacctgca
     7801 ccgattgcct caatctgatc atcggtcatc ttgtgttctt ttgcttccag tgctttgtct
     7861 acgcccatag cgatatcgcc ggactgctca tcgattgctg tgatcacacc gcaggtatca
     7921 gcatcaaagc catattttcc tctggtgtat ccgatctcac ggatcgtatc tcttacgatt
     7981 ttctggatat caacgtatgc ttttgtggtg atctcaccca taaccagtac caaaccggtt
     8041 gttgttgctg tctcgcatgc tacacggctc atcggatcct gctcaagcag tgcgtccaga
     8101 atcgcatcag aaatcgcgtc gcacattttg tccggatgtc cttctgttac agactcggat
     8161 gtaaataata atttctccat ttgtctctcc ttttttaata aatgaatgta tgatgccttt
     8221 gaactgtatg gaatttttac aattctgaca tcggtaattt tatagttcct gataatatga
     8281 aactacatgg tttcttttta taatcccaca gaatgctttt ctgtggaacg aaaaaaggtc
     8341 cacaaaaagc ggacctctta ttccaatgat aagttcaatc ctcttattgt tcgaatactc
     8401 gctcaggttg gcaccttcct ttaaggggtt gccgtgactt cacagatcct atgtatctcc
     8461 atcactctga ataagagaag ctatttaatt ttcaaccaac tctcagtttg aatttctgaa
     8521 tttccttttc ggtgctaact cgttcaatct cagcaccaag gctgcggagc ttgtcttcaa
     8581 aacactcata tccacgctga atatagacaa tatcatcgac aacggtgaca ccttctgctg
     8641 ccagtccggc gatcaccagt gctgctccgg ctctcagatc cggtgcactg actcttgctc
     8701 ctgtaaaccc atcggttccg tagataatag cagaattgcc ttctacttta atcgtcgcac
     8761 ccattctggt aagctcgtcc acatatttga aacgattctc aaaaatgctc tctgtcacga
     8821 tactggtacc gtgtgccaga gccagggcaa ctacgatctg cggctgcatg tcggtcggat
     8881 accccggata cggcagtgtc ttgacgtttg tgctatttaa acgtttggat gctaccacac
     8941 gcacagcatc gtcaaattct tctacctcac agccgatctc gacaagcttt gctgtcgttg
     9001 cttccagatg tttcgggatg acatttttaa ccatgacatc gcctctggtc gctgctgctg
     9061 caaacataaa ggttccggct tcgatctggt caggaataat ggaatactct gccctgtgaa
     9121 gcttctgaac accgcggata cggatcacat cggtaccggc acctttgata ttggcaccca
     9181 tgctgttcag gaagttcgcc acatcgacaa cgtgcggctc acgtgctgaa ttctcgatga
     9241 tcgtgtatcc ctctgccata gatgctgcca tcatgatgtt gatcgtcgca cctacggaaa
     9301 ccatatctaa ataaatatgt gttcctttca gcttttctgc ctctgcaacg atggaaccgt
     9361 ttcggatact cgttgtcgct cccagtgctt tgaatccttt cagatgcagg tcgatcggtc
     9421 tgctgccgat gttgcatccg cccggaagcg gtacttctgc tcttttatat tttccaagaa
     9481 gtgctcccaa cagatagtag gaagctctga ttttcttaat aaactcgtaa tctacactta
     9541 cctgaccgat catagaacca ttgatagcca ctttatgacg gctgatcctg tcaactctgg
     9601 ctccgatcac actgatcgct tcgagcagga cattgatatc ctggacatcc ggaagattat
     9661 cgattaccac ggtctcatct gtcataatcg ccgctgccaa aataccaagt gccgcatttt
     9721 tcgctccgcc gatttcaact tcgcccacca ggggattgcc gcctttaatt acatactgtt
     9781 ccatagacac ctctattatt aaaatatact ctctatctct gatgcatccg ttaccgctct
     9841 gcatcattat catcattttt caaattgctt tttttacttt ttaacgaaat tctgtaaacg
     9901 tccggaaatg acgtaaacac ctgcgaatat gtgccgagga caaaaagcga ttcccctaac
     9961 atcataattt ttattatacc acaaacttgg tgtttgtaaa caaaaattaa gatttcagta
    10021 aaaagcaacg aagatacatt tcaggaaccg tggattttag ctatattctc cgtaacattc
    10081 ttgtaacaac cgctattttc tgctccgata tcgcaaaacc acctgttcca tcgtctcttc
    10141 cacgctcatg ttcgtttcat cgacggtgat atcagcatat ttttcataaa gtggaatgcg
    10201 ttcttcataa aggctctgca aggtctggcc ttcctgaagt gcgacacctc tgcaccgcag
    10261 gtcaccgagt cgctcctcca gctcctcgca ggtcagtttc agataaacga ccgttccgat
    10321 ctcttttaaa tgctgcatcg cagaatctct gtatacaaca cttccgccgg ttgcgatcac
    10381 gcatctttct gcctcaatgg aagcattgac gcgtgcttca atatcaagaa atccttccgt
    10441 accaaattct tcgatcagtt ccggaagcct ctttccttcc tgactctgga tctccaggtc
    10501 ggaatcaata aaacgatatc ccagtacttt tgcaagcaca acaccaacgg tgctttttcc
    10561 tgaacccggc atgccgatca ggatgacatt ttttgaactc ttcatttctt accacctgcc
    10621 tgttttttca taactgtatc acagcagata tggtttgtaa agattgcttc ttaatggcac
    10681 atattttttc ccttctgcac gtacactgta aaaaacagtt caggtatctg cctatgtcct
    10741 cctttctttc cagacttcgc aggcacaaac ttctgcttgc ttttcttcct gctcttatct
    10801 ttctcttttg ttctgttttt tattatgaaa tcaatcattc ttttgtcgct ttctgtgata
    10861 cggtctttcg aaatgaaatt acagaaaata ctttaaatct gcactataca ctggaaaacc
    10921 cggaaatttt aaatctttct gatccggatg tttcgtttgg ttccatgaac ccggaagttc
    10981 tcgcagattc tttttcacag cagaatattt atctggaaaa acttttcaag tttcccaaag
    11041 actggctttc cacaaaaaac cgtctcactt atgatatttt aacaagtgct ttttcgttac
    11101 aggcagaggg tgaaaaatac ctgctttacg atgagccgct cggtccgtct ctcggcacac
    11161 aggcacagct gccggttctg cttgcagaat acagttttca taataaaaaa gatattgaaa
    11221 cgtatctggc tcttctttcc gggcttccgg aatactatga cagcattctg cagtttgaac
    11281 gtgccaaatc agctgccgga cttttcatgg atgcttctgt cgctcagggc gtgatcgacc
    11341 agtgtgatgc ttttcttaag accggcgatc agaatattct gtttaccact tttgacagcc
    11401 gcatcgcctc tcttgatttt ttaaatgaaa ccgaaaaaca gaaatactgc tctttgaatc
    11461 ggaaagccct cgcctcttat gttatcccga cttaccggaa actttccaaa gggatttctg
    11521 cgttaaaaga cagcagcaaa aacgctctcg gtctttgtta tcttccggat ggcaaaaatt
    11581 attatgctta tcttgtcaaa gacacgaccg gctgttatga cagcgtggaa acgattttta
    11641 aaagaatcca gtcacagctc gtaaaagata tccacacgct gcgtcagatc gcacagaaaa
    11701 atccacaatt attttccgat tccggggatg aaacgctgaa aacggtgaat gatcaggttt
    11761 cttctgatcc aaaggagatt ctgaatgatc tgaaacggaa aatggcagag gattttccgg
    11821 aaatcgcgga cgtgacatac gaagtcaaat acgttgaaaa agctctggaa gattatttaa
    11881 gcccggcttt ttatctgacc gcaccgctcg acaaccgcaa ccagaatgtc atctacataa
    11941 atccgaaaat cggttaccgt ggactggatc tctattcgac acttgcacac gaaggttatc
    12001 cgggtcatct gtaccagacg attttctcga acgactccgg tatccgccct gtccgcagtc
    12061 tgacaaattt cggtggttat gtggaaggct gggcaaccta cgtggaaatg cagtcatttt
    12121 cttacggatg ttctgataat atcattgccg aaatctatcg cctgaaccgt tctttaaacc
    12181 tcggcatctc ttcttttctt gatattctga tccattatta tggatatgac cgctcgcaga
    12241 cggcaaaggt tctttcgcag tttggcttaa atggagaagg gactgccgat gcctttttca
    12301 acaccatttt ggaggctcct gcaaactatc tgaaatacta tgttggctat ctttgttttg
    12361 aagatttaaa acaggcagcc ctgaaaaaag aaggtgcttc tttttctttg aaaaatttcc
    12421 ataaaaaaat actggaaaca ggtccgtgcc catttccagt attgtataaa tacgttctgc
    12481 aaaaatcata ataaattttg cggttatttc catgatgcca gggtctaaag gtcaaaatgt
    12541 cgttcttgct tgcaagataa gacatttgaa cttgagaccc gctaaacatg aggaagtagc
    12601 tatttacgca gtaaatacgc ggattccgaa tgtttatagg cactgagtgc cagtggcact
    12661 cgtttagtgc cgaccgaagc ggagcgtaga ttgcgggagt tactttgtaa cggagcaatt
    12721 cgtaggcatc aatcagggtc taaaggtcaa aatgtcgttt attgctgata ctgctcaaaa
    12781 aagccgatca gattggtgag tgcctgtgcc agcacttctt cttcctgttc actcatattt
    12841 gcaatcgtcg ccttgatcat tttgtcgtga aagaggcgat gatgctcata tgctctccgg
    12901 cctttttcgg taagagaaat cagcacgacc cttcggtctt tttcgctgcg tgtacgattt
    12961 acataacctt tttttacaag attgttaacc gcaatcgtca gcgttccgac cgtaacagaa
    13021 agttttttgg ctacgaaaga catatttcgc ggttcatcaa tgccgattgc ctcgatgata
    13081 tgcatatcgt tattggagat ctcgctgaat tcccccgtcc ggatcgcttt ttcttccagt
    13141 gtcataatat catggaacaa tcgaaccaga acattattta acgtgtcgta tgtcgtactc
    13201 acagctttcc tccttctgct tttcaggctt tgtctctgtc aactaattcc cggtagacat
    13261 ctctttttcc gactccgcgg tcttttgcta cctgtttcat cgcttctttt ttatccatgc
    13321 cctgatccag gtatttctgc atatgttctt cgatgtccat ctctttccag gctgcctgac
    13381 tctcctcttt catctgctgc cggcttctgc cctcgagcac cagtacacat tcgcctcgcg
    13441 gtgcttccgc ttcatagtac gccactgcct ctgaaagtgt cgtggtgaat gctgtctcat
    13501 gctttttcgt cagttcccgc agaaccgtca ttctgcggtc gccaagtgtt tccagaagtt
    13561 cttttaaggt acgcaccagc ctgtgcggtg cttcgtaaat gatgatcgtc ctggtctcgt
    13621 cttttaattc ttccagaact tcctgttttt cttttttatc cgatggaaga aatgcctcaa
    13681 aacagaaacg tctggtggat aatccggaaa gtgtcagtgc cgtcacgcag gcacaggctc
    13741 ccggaagaga agtcacctca acaccgtttt catagcacag acgcaccaga tcttcacccg
    13801 gatcggaaat gcccggcgta ccggcatcgg tgatcagggc gatgtctttc ccttttttca
    13861 tttcttctat taaaacatag gctttttcga ttttattgta ttcgtgataa ctcgtcagcg
    13921 gagtatggat atcaaaatga tttaaaagcc ggatgctgtt tctggtatcc tccgccgcga
    13981 tcagatcgac ctcctgaagc actcgcagaa cacgcatcgt aatgtcttcc agatttccga
    14041 tcggagttgc acacagatac agttttccct gattttcgct catatatttc tcacctttcc
    14101 gaatgctctt tgaccctagc atcattgtat ccgtaaatat cataaatctg ctttgtatac
    14161 acgcccggtt tctcatacac gatcagcggc ggctcacagg tgatccttga ttttgcacct
    14221 ttcatggctt cgatcaggac catgttcggt tctttatcga tatatggatg caccatctgc
    14281 attctctttg gctcaagtcc gtacttcaca agcagataaa aaatctccgc cagccggaac
    14341 ggtcgatgta ccagataaaa ctttccgctg tttttcagaa gttttgctgt ctggacgatg
    14401 atgtcctcca gcgtgcagta gacttcatgc cttgcgatca tttttgcatt atccggattg
    14461 accagaccgt gatgctcgat catatacggc ggattacttg tgactacatg aaaagaagcc
    14521 gaagcaaaca atgtcccggc ctccttgata tcgccttcta caatagagat tcgctcttcc
    14581 agatgattat accgaatgct tcgcatcgcc atatccgcac tctcatgctg tatctcaaga
    14641 ccggtaaaat gtccggcttt tgtctttgcc gccatcagga tcgggataac tccagtcccg
    14701 gtaccaatat ccagaacgtt atctcccggc tttgcctttg cgaatccgga aagcaaaacc
    14761 gcatccattc caaaacagaa ttttttcgga ttctggatga tatggtaccc gcttctctgc
    14821 agatcatcca gtctctcatc cggatacagt ttaattgtca tttagttttg attttccttc
    14881 ctttttctcc attgcttcga gtttcttcag ctctttttct tcctgtttcc ctgccccgcc
    14941 gtttttttct tttcttctgc gaggtttgaa cttgagctgt ttgacatcat attcacggat
    15001 ctctttttcg tccccgactt ccacaaccac ttttacggtc tgacgaagca cgttgacact
    15061 gtgtacttct ccttttaaac cgtcctcagt cgtcaccgta tcattgaccg ccggcagacg
    15121 gctgttcaga tactcgtaag tctcctcttc atttttcagg cagcacatca gtcttccgca
    15181 gacaccggag atcttggtcg gatttagtga aagattctgc tcttttgcca tcttgattga
    15241 aaccggtgcg aactcagata agaatgtatg gcagcaaagc ggtcttccgc agatgccgat
    15301 acctccaagc agttttgtct catcccgcac accgatctga cgaagctcga tacgtgtctt
    15361 aaatacggat gccagatctt ttaccagatc acggaaatcg attctgccgt ccgccgtgaa
    15421 ataaaacagg actttgttgt tatcaaacgt atattctgca tcgatcagct tcatatccag
    15481 cttatgtttg cggatcttct cggcacagat acgcatagca tccttttcct ttgccaaatt
    15541 gcgttctact cttgcatcgt catccggtgt tgcgatacgc ataacggctt tcagcggctg
    15601 aactacttcg tcatccggaa tctcctttgg attggctacg acgctgccgt attcgattcc
    15661 tcttgctgtc tcaacgatca catggtctcc ctttttgatc ggcagttttt tcggatcaaa
    15721 gtaataaatc ttcccggccg tacgaaaacg gacaccaaca atttttatca ttataccaaa
    15781 ttctccttta taagaagaaa cagcagttcc atggtcagat caaaattaac gtttgcattc
    15841 agacgcgttt tcgctgtctg gagtccccgc agaatatttt cgataccttc gtaggaactt
    15901 ttattgatct gatcggtgat cacattcagc tcatctttaa aaatgagccc attgctgtct
    15961 cttgtcgctt taaaatacag gatgtcacga taccacaccg ccagaaaatc cagataatcc
    16021 tgaatatcaa tcttaaattc ggatacggat ttcaccgttg cgataatatc gttgatctcc
    16081 atcttagata catttttcag cagatgaagt gctgaatttt tgatttcatt aaagctgtcc
    16141 gatgttgcca gctgtacggc ttttccgata ttgccctgtg caaatgccac gcagacatcc
    16201 gcctcataat ccggcacnnn nnnnnnngcg atgaaacttg ggaaggtgtc ccatgcgtat
    16261 atattaagtg gagaaaaggg atgcggcaaa aagctgctgg cagatgtttt tgcggaaaca
    16321 ctgcagtgtg aaaatggagg cacagagcct tgtggcagct gccattcttg tgtacaggca
    16381 cagagcggca accatcctga catcatacat ctgatgcacg aaaagccaaa cagcatcagc
    16441 gtggatgatg tgagaacaca gattgtgaac gatgtattga tcaagccata cagtggaaaa
    16501 tataaaattt atattgtgcc ggatgcggag aaaatgacag cggctgcaca gaatgcactt
    16561 ctgaagacaa tcgaggagcc gcctgcatat gcagtgattc tgctgcttg
//
LOCUS       DAWDWH010000020        15618 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_2167_length_15618_cov_17.171754, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000020 DAWDWH010000000
VERSION     DAWDWH010000020.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 15618)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15618)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15618
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2167_length_15618_cov_17.171754"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4736..4745
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3639 a         3852 c         3125 g         4992 t
ORIGIN      
        1 gtgcggcatt tcagttcttt acacctgccg ggaaaccaac ggtcaatttt tatttgtcaa
       61 atgtaatttc tttatgatac actgcatgtt ctgcaatata ttcttcatag gttttcgtct
      121 ttttcttttc ttcgtaatct tcggagaagt aatatccttt cagttcgttc atatattcat
      181 cttcatcgca gaggtaaata ttgactttcg aggtatcata cccgtaggtc tggcggcagt
      241 cggtaatatc tccggcgtag tccatgcgtt ctccgtttgc atcgaagact tctgcgaaat
      301 agtatttgcc gtctgtctcg cttttgtctt tcggatatac tcgcagttca tatctggttt
      361 ttacgacttt ttccagtccg actccgtcat cgttgacttc gtttacttcc acggtctgtg
      421 tatcttttgt atccagtttg acattaagat caaagtccca gtttccttcg taaatttttt
      481 catccgcaac cgtgaagttt acattgtcaa agcgaatgga atccagtgcc atatggcagg
      541 taaaggtatc ggtcggttct gtctccagag aacttaaatc tactcgcagc ataccggcaa
      601 aggtgtgatc gtccacaaat tttccttcca tatagttaaa taagaatgtt ttttcatccg
      661 gcatgaagct aaaggtattc tctccgctga tggctgcctg gtcaaagtct aaaatatagt
      721 ctttgacatg atctttgatt tttatatctt ctggaaatgg ctcttcactg taaaggctca
      781 tggcaatgta catggcattt ttattatagt aaacttcgga taatgtcaca gtaatgccat
      841 tggatgtctt tgtataggct ccatctgccg ggacttccga atcatcatca attcctgtat
      901 tgccattttt gctgtctgca ttttttactg ttttgtccgc tttctgatct gctgtgtttt
      961 ctgtggactg tgtcagagtt tctgcaacct gactgaaatc tccttttacg ctggtgttgt
     1021 tttccacctg ttcaaaaatg tgaccgatca gtggcatttt tgctgcaaaa gatggattgg
     1081 aaatacataa caccgatgcc agtgccaccg cagctgccat tcccattcca acttttgccg
     1141 tttttcgaaa cgctattttc ttctgtctct ttttcccctc ttcaatgcct gcccggaccc
     1201 gctgatccag atcttccggc accggaatct tatcaaagcc ttcgatctgc tccggatttt
     1261 cgatatattc aaatgttttc tgttcttttt tctgtttctc tctattcatc attacgaacc
     1321 cctacccttc ttctaagatc agttttaatt ttctttttgc tctggaaaga tctgatttaa
     1381 cagtcccttc tgctcttccc gtataagctg caatttcgga aattttctta tcttcaaaat
     1441 atttctgttt tatgatagtt cgatacggct cttccagaga ctcaactgca ttgtgcagat
     1501 cgatgatctc ttcacggctt gccccaccat tctcttctgc tctgtcccgt atctccgcat
     1561 ccaggcttcc gatttctttc tccgcttttc ggaacatttc gtttgcacag ttataaataa
     1621 tgcgtgtcat ccatgtgcgg aaatagttcg gatttttcag atctttgatg gaaagcatcc
     1681 cgatggtaac ggcttcctgg aaaacatctc ccgccagatc tacatttttt acatagcaga
     1741 atgcattccg gtaaaaatag tctttatgaa gctgtatcaa ttctccgtaa gcttttgtat
     1801 cgcctttttt ggctttccgt atcagccacg cttcgctgct catgttttat cacctcccag
     1861 gttctatgcc ggattttttt gtatcctgca ttttctgtag gttagatgct ctccaccggt
     1921 aaaaggttgc aatgatgcaa aattttttgc ccccaacatc attttcataa aaaggacggc
     1981 aaatttaaaa tctgccgtcc tttttatttt agatgccagg gtaagcgtac gaagtatgaa
     2041 ttcgtatgac acctttttaa tcttaacctc tttatttttt attcttctga agaatctctg
     2101 tagtagttga tcagacatcc tttgaggata atcgttcttt catcatccgt cagctcatcc
     2161 attttcaggg tgatttcttt taatccatct ccgacaacat aagctttcat ttcttttgct
     2221 ttgtctttga ctgcctgttt tacgttcggg ataaacaggt aatcgccgtt tgtaaacgga
     2281 agctcccctt tgtcaatgat aaatggaagc ataccccagt tgatcaggtt ggagcggtat
     2341 ctctttgtcg catattcgtt tgcgatgttt gcccagccgc ccagtacttt ctgacaggat
     2401 gcagcctgct cacgtgcgga accatctccc ggttttactg cgaagatcgt gcttccgata
     2461 caggtatttt cactgttcat atccggatat gcttttttga tctcatccca gactggtttg
     2521 agttcatcaa aagcttcaca tggacagttt ccggcttcgc gggctttttc tgctttctgt
     2581 acttcttttg cacgtcctac atatgccgga tcttttctgg agagtgcaaa ttctgccagt
     2641 ccaagcgggt tggaacggta ggaagatgtc tctccggatg ggatcagctc gtcggttgtg
     2701 gtaaccggat cgtggatctc ggatacgact ttcaatacca tattttctgc cagcggtgcc
     2761 atttccggcc agtctttgat gttcggacca aactggatct cctgatccgg atctgccacg
     2821 cccttgctgt caaatacacg gtttgcgtaa atagaactgt cgaagtaata ttttggattg
     2881 ctgaaatcta cgtccacatc ggtagccgca gtcaggtatc ctttgtttgc tgcagtagct
     2941 gcgatggaac gtgcatccat cagtgctacg gaagaaatct ggccattctg cagtttgctt
     3001 ccttcacggt tcgggaagtt acgtgtagag tgacggatac tcagggcatt gtttgccggt
     3061 gtatcacctg ctccgaaaca tggtccgcag aatgctgtct tcacgatcgc acctgctgcc
     3121 atcagatctg ccagtctgcc gtttcttgcc aattctacat agatcggagt acttgccggg
     3181 tatacgctta acgtaaaggc atctgctccg atgttggaac ctttcaggat atcggctgcc
     3241 gcacagatgt tttcaaatcc gccgcctgca catcctgcga tgattccctg ctctacatag
     3301 agtttgccgt tatgtacttt atctttcaga gaatattcta ccgctccgcc aaggctgact
     3361 tttgcttttt tctctacatc gtccagaata tccatcaggt ttgcattcag ctcgtcgatg
     3421 gtgtaggtgt tgcttggatg gaacggcatc gcaatcatcg gacggatttc gcttaagttt
     3481 acataaacca tgccgtcata gtatgtaact gcacccggat tcagtgcttt ataatcttca
     3541 cttctgccat ggatatcata gaaatctctg atcttgtcat catcggttct ccagatggag
     3601 gacaggcagg tcgtttcggt tgtcataaca tccacaccga tacggaaatc tgcactcagt
     3661 ttctctacgc ccggtccgac aaattccatg actttgttct ttacatagcc attttcaaag
     3721 acagctccga tgatcgccag agcaacgtcc tgaggaccga cacctttcat cggctcaccg
     3781 tccagataaa tcgctaccac acccggcata ttgatatcgt aagttttgga gagcagctgt
     3841 tttacaagct ccggtccgcc ttcgcccatc gccatggttc ccagtgcacc gtaacgtgtg
     3901 tgggagtctg atccgaggat catctttccg ccgcctgcca gcatttctct tgcgaactga
     3961 tggataactg cctgatgagg cggtacatag attccaccat attttttcgc acaggtaagt
     4021 ccaaacatat ggtcgtcctc gttgatcgtt ccgcctactg cacagaggct gttatggcag
     4081 tttgtcagta cataagggat cgggaatttc tccagtcctg acgctcttgc tgtctgaatg
     4141 attcctacaa acgtgatatc gtgagatgtc agtttgtcga acttaatctt taatttatcc
     4201 atattaccgg atgtgttgtg ttcctgtaat atattataag cgattgtatt cttcgcagcc
     4261 tctgcttttg aaggaacacc atcgcccatt ttttgtttca gtattgcttc tgcatcatgg
     4321 ttatcttcca cgatctcggt gccgtttacc agataaactc cgccgtcata taacttgacc
     4381 atgttctacc tcctgaaaat tgaaatctta ttcacgaaac gggcagaatt ctgtcttttg
     4441 cccgcatagg taaagtataa tcgcatccgg ctaaaaaagc aagatgcgat tgttttcggc
     4501 atcaaacttc tttgcttcat ctttatcatg tgcagcataa agtatggttg tgccgtatct
     4561 ttcatgtaat ttttctattt ctgtccggat ctgaacctga actttttcgt ccagaccatt
     4621 taaaggctca tcaaacagaa aaagtttggg tttgcggatg atcgccctgc caagagccac
     4681 cctctttttc tgtccttctg agagaatctt cagcctgcgg tttaacagtt cttccnnnnn
     4741 nnnnncagca tatctgctgc ttcttttaca gccgcttcga tctgctccgt ggaacatttt
     4801 ttcagtttca ttccaaacga aagattatcg taaacggtca tatttttata cagactgtaa
     4861 tactgaaaaa ccatggcaat ctcccggtct tttggttcga cgtcattaat cggttttccg
     4921 tccagttcca ggatgccttc cgtcacatct tctgctccgg cgatcatacg aagaacggtg
     4981 gattttccgc atttcggaga accggtaagt atcacaaact ctccgtcttc catttcgaga
     5041 ttaaaatctt ttaccgccca aaatccattt ggataccgtt tgctgacatg cttaagggag
     5101 agctttgcca tattttttct cctgttttta ttttgtgttc ttgaagttat ccatcagctc
     5161 ttccagctca gcgagcagac agtctactct gccgtagaaa gcgtcgttat tggtcggatt
     5221 atccagctct ctttcgatat gtatcagctc ttctgtcagt gcatggatac ggttctttcc
     5281 tgcattgtct ttgtagagca tgtaacggct tctctcattt tcttccgggg tcatcttttt
     5341 cagaacaact tctacgtttg gtttttctcc gatataacgg taagtgatcg atggtgatgc
     5401 atggttcaga agattctgga tatagtaaat atcgtttgtc tctttgtaat atctccatgc
     5461 aaaggtttta cgcattgtct gtgcaccgat cgcattcagt cccatgctct ttccgacgtt
     5521 tttaaatacg cggtaagcct gttctctgga aagcggtgcc ggtgaaccgg aatgtccgag
     5581 gatcagataa gcctccggat ctttgccttc tgtatactgg ctgatgatct ctttgagttc
     5641 ctgcggaatc tgaaaagtac gtttgatatt ttttgttccg atacttgctt caatgctgtc
     5701 tttatccctg atgtctttgt tgcggaattt caggatctcc tgaagctgca gaccggtccc
     5761 cactcctatc tcaaataaaa tatagtactt gtcatctacc tctcttaatt tctgtttaaa
     5821 tttttcaagt gttgtatcat ccttgatcgg tgctacccag ttcatactct ttttaatcct
     5881 cctcttcttt tttcagatcc tctgtttttg gctgttctgc cggcaggatc tcttcttcgg
     5941 gtttttctgt tggttttatt tcttttgttt cttttgtttc ctgtgcttct tttgctttct
     6001 gccggtcgat cagctcttcc atttcccgta atcgcttctc ttcttcttcg gcttctcgct
     6061 ttctgcgttc ctctttctca agttctgaac gaatctctga ctcgatctct tggaaagatt
     6121 tgctgtctat attttcttct atctttcttc gctgttcttt gtcctgctcg cgttctttcc
     6181 ttgccagctt atcgtttttg tcacgcatgt aagcgatgac gataagcggg atcataagca
     6241 ggatcaggat catccctacc gtcgcagaca gcatttcgtg ctgatctacc caaccgttga
     6301 aggatgtcca tatattcagg aatccaaatg ttatattatc cataaattca tcaactttct
     6361 cttcgaaccc tttgctgccg gatgcatctt tttccggtgc ttctgtactt cccgctgtct
     6421 ctgtttcact gccatttgtg tctgtctgac cctgcagtgt cgcatctgaa atttctgtct
     6481 ttgtcacctc aaatgccgga ttgttaaact ttaaggtttc ctctccgact tcccagccct
     6541 gataactgta aacaatcttg tttccgtcac agaaacctcg cttcagcttg tccgttttta
     6601 gttttttcgg gatcgtaacg cttacggtac ttttcccttc gaccgcccag ataccggctt
     6661 ctttctgatc cagaacggat gctgtgccga aatgacagat acctgccagc tgtccgagtg
     6721 ttgtcgtatc aagagccgca tcgatctctt cgtggcggaa tttgtcgaac ccgtaatcta
     6781 aaagttccgc agattcatta aaggttttcc aggaaccatt gacgcgaagc tcaacacaca
     6841 ccagcgtctt gccgtctttc tttgcataag taacgagcgt gttccacgct tcgtccgtaa
     6901 atcccgtctt gccgccctcg cagtctttgt aagtaaactc accgtcttca tagagcattt
     6961 tctggtgatt gaccagccag cggtcttctt tcatatattt tgttttcggg attttgtatt
     7021 cgactgtttt tgttattttc cggaacagcg gaatctgaaa ggctgcctga gcaatcagtg
     7081 ccatatcgtg ggcacaggta taatgttttt tgttgtgcag accatgcgga ttgacaaagt
     7141 gggtgttgac gcagccaagc tcttttgcct tgtcattcat catcttcaca aaattgtcca
     7201 tcgatccgcc gacgtactct gcgatcacat ttgccgcatc gtttgcagat gccagcatga
     7261 tcgcatacag ggcagaacgg actgtgatct tctccccgac ggacagccag atctggctgc
     7321 tgtccgaatc gatcggagca agtgcactct cagatgcttt cacttttgta tccaggtcac
     7381 tgtcactgat gtgctccaag gcgaccagca tcgtcatgat cttggtgatg ctcgccggat
     7441 acagttttgc ttctgcgttt ttgctgtaaa gaaatgcccc tgtatccaga tccatgacag
     7501 ttgcaccttc tgcttctacc cggggaccct ggggccattt tttcaattca tttgtctcta
     7561 tcgtccaatc atatgcctct ggagtttctt ctacatattc ctccgtctct tctgtgtacg
     7621 tctcatcata cacctgttca tcctgggcaa tatcttctgc cgcaaacaga acagtcggag
     7681 aaatggttgc aagtactatg gcgatgataa gaagacatga gagaaaattt cttttcttca
     7741 tatgctatct ccgttatttt ctgaagtgca gttatctgct ttccttttaa cccttggttc
     7801 taagtatatt ataatcatcc tgttttttca agattgttat tacaggataa cactgtcaaa
     7861 aagtatcttt tgtcattttt tcttttattt tgcagaaaat aaccgccgtt gccgtgctta
     7921 ccagtgttgc tgcgatagcc gctccggtga tggcagaggg attgctgctt ccgtactgac
     7981 tgcgataggc gatcacgctg accggtatca gctgaaggga agaaatattt aagatcagga
     8041 aggtacacat ctcattgctt gcgacttctt cgggcagacc tttttctttt tttatttctg
     8101 ccagttcttc catcgctttt aatcccgccg gcgttgccgc tgatccaagc ccgagaatat
     8161 ttgcgatcat atttgccgcg atatattcct gtgccttatg tccttttgga atccccggga
     8221 aaagaaaatg gataaacggc tttaattttt cggaccattt tctgatcagt ccggctcctc
     8281 tggctatctc catcacgcca acccagagtg ccatcattcc ggtcatggtg atgcacagcg
     8341 tcacggcatc ttttgccgat gcaaatgccg catctgtcac tgcctgcata tttcccgtga
     8401 ctgccccata gacgatccca agcagtatca ttcctatcca gagattattc atattatagt
     8461 tttcaccatt gtgcttttat ttatcacagt ttatgcactt ttttcataca aaaatacatt
     8521 ctctgttctt tttccggctt gactattttt tccgctgcat ttataatgaa atgcgtattc
     8581 gcatggagac gtagcgaagt ggccgtaacg cgcctgactc gaaatcaggt tgtcctccgg
     8641 ggcacgaggg ttcaaatccc tccgtctccg ttaactattc aaattaatat aagtttatac
     8701 aaaaaagtac tgtacggctc attctattca cagtgctttt tcttgtataa tggaggaaaa
     8761 tctatgcaga aacaggattt taagaaactt tttgaacatg gacctgtaat tctggacggt
     8821 gcgaccggct ctaatctcat gaaagccggt atgccaaaag gtgtatgtac tgaaaaatgg
     8881 gttctggaac atccggatac gatcatccag cttcagagag cctatgcaga agcaggatct
     8941 cagatcatct atgctcctac tttcgctgca aaccgcatct ctctggcaaa tcacggtctt
     9001 tctggtcagg tagatcatta taaccacgcc cttgttgctc tctcaaaaga agccgtacag
     9061 ggaaaggctc tgatcgccgg agatatcacc acgaccagca aaatggatgc agaatacgat
     9121 gagcttcttt ctgcttatga agaacagatc acagctctgg tggatgcagg cgtggatctg
     9181 ctgatcgctg agacgatgat cggtgcagac gaaaccatgg cagtgatcga tgccgcacat
     9241 gctgtctgcg accttccgat tctctgttct ctgaccatgc aggctgacgg cagtcttttc
     9301 tttggcggaa atattttcga gacagcaccg atgctcgaag agatgggtgc agatgcggtc
     9361 ggtatcaatt gttctacagg ccctgatcag ctcgaaaata ttatccagaa tcttgccggc
     9421 agtctctctg ttccggtcat cgccaaacca aatgccggta tgccgacgat caacgatcag
     9481 ggaattgctg tttatgatat gacaccggag tatttcggca aaagcatgaa acgtcttgtt
     9541 gaccttggtg cctccatggt cggcggatgc tgcggaacag atccttctta tattaaggca
     9601 cttgtaaacg caatccgcta aattttctct tacccggatc gtatccagac cgaacgtatg
     9661 tgaggctttg caattttatc agccgatcag gtattggcat aataaaaaga catacatcgc
     9721 aggatttttc tgttttgtat gtctttttta tataatcatg aattttttct tattttattt
     9781 gcttgcattt ttttaccacg caccgcactt tgaacttccg ccacaaacgt tactcctaca
     9841 gttagctcaa tccaggcacc ctcgcgtcac acaaaaggtt ctgcttagtg ctgctgcatt
     9901 cctgccctga cacggttcac agatttctgt tgcacgagac ccaaacgtca tcaccactta
     9961 tagaaggcag ctttgcagct aaaaaccctc ggattggtat cacccctgct gtagcggatt
    10021 gcaggtacag ggcaccgcta tctccccggc tgcgtggtct aagttactat atcgtgaatc
    10081 cgctgttttg tcaagtttta cttcggattt taccccatca gccaccaagc tctaccatgc
    10141 ggttgataca tcttcctgct ccttcgagca cgttttgcgg aaggtggatc tcttcgccgc
    10201 ctcttccttt caggcagtta tagacatcca taagtgttgt gatcttcatg ttcatgcagg
    10261 tgagcggaac tgccagcgga tagaattttt tatccggaca ggcaaactga aggtgttcta
    10321 caatgctgtt ctctgttccg atgataaatt cttctttatc ggatttcttt gcaaaatcca
    10381 tgatccctgt ggtagatccg acataatctg ctttttcgac aacttcctga cggcattccg
    10441 gatggaccag aagcagtgca tccgggtgat ctgcccttgc tttttcgaca tcttttgcac
    10501 tgactacgat atgacgcggg cagccacctt tataaaaagc aaaggttttt tccggaagct
    10561 gttccgctac atagcgtccc aggttcggat ctggaataaa caggattttg tcgttttcca
    10621 gatgactgca gatttccact gctgaagaag aagtcacgca tacatcacat tctgtcttga
    10681 gttctgatgt tgtattgata tacgccacaa ccgtatagtc cggatattct gcttttaagg
    10741 tgcgaagacc ttccagatca agctgttctg ccatcgggca tccggctgca gggtttggca
    10801 gccatacttt tttatccggg cttaaaattt tacaggtctc ggccataaaa cgcacaccgc
    10861 acatgatcac gccgctgcat ttgcttttgg atgcctgtac gctcagtccg taagaatctc
    10921 ccatataatc tgctacttcc agaatttcct gaccttgata agcatgagcc agaatacata
    10981 catcctgttc ttttttcata cgcaggatct cctgctgaat ttctgctatt gtcattcttc
    11041 gtttccttcc tgttttgtat tctgctccca tggctgaagc ggtgccagga gtcctgcttt
    11101 tttcagttgt tcttcgatca gatccagtct ctcttcgctc gctgcttcaa ccagatgata
    11161 gtgataaccg gatgttgcat tactcagtgc actggaatta ctgttgttca atgcggtaat
    11221 aaattcattt acatcctgtc ttgacctgat atccatttct gcactgactc ttccatagac
    11281 tctgtggctg atactgatat tgcagatcct gccgccacag tccacgatca gattcatttc
    11341 ttctgctgtc tgatccgatt catgacgaac tttaaattca cgaacgcagc tgctgtcctg
    11401 ctgtatcaca tatcctcttg ttgttgataa aattcccgga gtcgctgcac gaatcactgc
    11461 catatcctgc acgatcacct ggcggctgac tccaaactgg ctcgccagtt tccttgcagc
    11521 aaccggttca tctgcattct gaagaaagtt taaaatttca tttcgtcttt ccgcagtatt
    11581 catagtccca gctcctttca gactgcatga aggttcttca tggaaatgtc cagaatcgga
    11641 gcagagtgtg tcaacgctcc gctggatacg aaatcaactc cgatttcacg gatgcgtgca
    11701 atattttctt ttgtaatgtt tccggagcat tccgtctgtg ctcttccatc gatcagttcc
    11761 acagcctgtt tcatcacttc cggtgtcatg ttgtcgagca tgataatatc tgctccggcc
    11821 tccaccgctt cttttacctg ctccagggtt tctacttcaa tctcgatttt gcgtacaaac
    11881 ggagcatatt cttttgccat tgtgattgct tttgtgatgc ttccggctgc accgatgtga
    11941 ttatctttca gaagtacacc gtctgataag ttgtatctgt ggttgcatcc tccgcccaca
    12001 cgcactgcat atttttcaaa tacacggcag tttggcgtcg tttttctggt atccagaagt
    12061 gttactttgc ttccttccag aagttttgcg acctgtcttg tataagtggc aattccgctc
    12121 attctctgca gataattcag tgccacacgt tctccggata acagtacacg gatgtctccg
    12181 gtaagcgttg ccatcagctc accttttttt acctcatcac cgtctttgca tttaaaatca
    12241 atttccgttt tctcatccaa catctgaaaa actcttgcat agacatccag tccggcgata
    12301 attccgtctt cttttgcgat cagatctacc gttcctttta cctcggtcgg cataactgca
    12361 tttgtagaaa cgtcttcact tgtgatatct tcctgcagtg ccatacggat cagctgatcc
    12421 gcctgcattt tcatagttat actattcatt tgcttttttc atcctctcta tctcattcag
    12481 cacttgttta tgataccctt ccaaaattac attctggtct tcatattgat tcagatccag
    12541 tcggtccggt ctgcacatct ccggtgcttc tccaaatatc atatcatctg ctgcccgtct
    12601 tgcaaagacc agagattcca gaagcgaatt gcttgccaga cggttctttc catggacacc
    12661 attgcagctt gtctcaccgc aggcatagag tccacgcatg gaagtccggc tgctcaaatc
    12721 tgcctggata cctcccataa aatagtgctg tgccggaaca accggaatcg gctcttttct
    12781 cggatcaaat ccttcctcaa tacagtgctg ataaatgttc gggaaatgct gcatgatcgt
    12841 tttttcaccc agcggacgca tatcttccca gacaaaatca gtaccgtcct tttccatctg
    12901 ctcaaaaatc gcctgactga gcagatccct tggctgaagt tcattcgtaa aacgctgacc
    12961 gtttttgtca taaagaagtg ccccttcgcc acggacagat tctgaaatca ggaaacgtct
    13021 tcccggttta tgtgaaaata aagttgtcgg atggatctgt atgtaatcca gatgctctaa
    13081 tttcacctga tgtttcattg caataccaag tccgtctccg gcaatatgcg gatagttggt
    13141 agaattctgg aaaagacccc cgataccacc gcatgccagc acaacaaact gggaacggat
    13201 cggataaaca ttattttttg catccattgc aatcacaccg ccgcagatat tgtctttcga
    13261 tatgatatct accatcgtca tatactcaca gatttcaata ttttcttttg tctttacctg
    13321 atccagaagc gtctgcgtaa tctcttttcc ggtaatgtcc tcatggaaga ggattcttgg
    13381 tctggagtgt gcaccttctc ttgtaaatgc aagctcgcca tcctctgcac gcacaaaatc
    13441 tacatttctc cacagcagat cacgaatgat atcgttggag cttcggatca tcagattgac
    13501 cgctctttta ttattttcat aatgccctgc acgcatggtg tcttcaaaat aatcctcata
    13561 atcatcttca ccacgcagca tgcagatacc gccctgtgcc agaaacgaat ctgacagatc
    13621 cgccccctgc tttgtaagca tcagaatttt ttttcttttt ggaagattca gggcacagta
    13681 aagacctgcc gctccggtac ctacaataat ggcatcataa tattcattca ttttcttata
    13741 cctctttcct gtcacggatt tgttttgcca tgaacgacat tatactactg gtctttgcac
    13801 gtgtcaagac acctgtatat tttctactgc cttcctgttt taactcccat tacttttttc
    13861 atggcatcaa agaagaatct ctgctctttt gtaaaaagaa ctgctttcac tcttttttca
    13921 cggtttactt tttcttgttt ttcctctata atcaccatag agcaaccagc catgggagtt
    13981 actaagtttt cagaaggggc aaagtcatgg ataaaaacaa attaaaccac aatgctgaca
    14041 ggggcagtaa caaacagcag cagctgtacc taaacatcgc ttttttcggt gttttcgcct
    14101 gtgccgccct gattttttta ctgctggctt ttgataaaat accgccagtc agtgaacaca
    14161 aaaaagcaga aaaagaaacg ttatcaaccg aagctcagtc cgagaccgga accgagtctg
    14221 ccacaccgaa agaaacactt gctgctgaga caagcatcac gatcagcctg atgggtgact
    14281 gcactcttgg ctctgctccg gattctgacc agtacaacaa ttttgatgct tatcagtccg
    14341 tatatggtat tgattatttc ttccagaatg tgaaatctta cctggaaggg gatgatcttt
    14401 ccattatcaa cctggaaggt gcactgacaa ccgctgagaa ttcagccagc aagagcgatg
    14461 tttataaagg cgatccttcc tatgtttccg cactctctaa cagttcgatc gaggctgtca
    14521 accttgccaa cgatcacacc gaagattacg gaaccgccgg tctgaccgaa acacagacca
    14581 ctctgaaaga cgcagggatc acgacttttg gcaacgacga tacctctgtt cttgagatca
    14641 aaggcatcaa ggtcggactt gtcggtatat ctgccattga caaacaggta agtgagtgca
    14701 ggacacagct gacaaatgat atcaatgcag taaaagaaca gggtgcaaac ctgatcatcg
    14761 taaatttcca ctggggagcg aacggcgaga caacaccaga cagcgaccag acaacactgg
    14821 cacacgcagc gattgatgca ggggcagatc tggtagtcgg acatcatcct cacgttctgc
    14881 agggtatgga agtttataat ggaagaacga tcttctacag cctgggaagc ttctgcgatg
    14941 gtgtaaatat gtacccggac gacatggata ccgttatttt ccagccgacc tttactttca
    15001 gtgcaggcaa agagctgacc caaacaacga attccatcat tccttgcacg atttcttccg
    15061 acagcacatt caacaactat cagccgacac cggccgaaga cagtgagaag accagaatcg
    15121 aagaaaaagt caaagaactg agcaatcaga tcggaaacag catttccgga gataattccg
    15181 atgcaaacag tacatctgac tccgcaaata ccacggattc aaacagcaca tccggctcag
    15241 atggaaatac aaccgcttcc tctgaaagtg agacttcttc cgagagcgaa acttcctctg
    15301 agacaagttc agatggaagc agcagtgatg gcagcgataa ttccgcttct gacggaaacg
    15361 actccacaaa actgttttta ccggaataaa aatttaccgt tggtttcctg gcagatgtaa
    15421 ggaatggaaa tgccgcacat tttaaaatac tgtttgcaaa tgaacagtat tgcatgtgca
    15481 aatgaacgaa ttccccgaag ggcacgtaaa agtgccttct tgggaattcg ttcattttgc
    15541 acattgccat acgtttttga acagccatag tttttaaaaa cacttcatat catattttac
    15601 acgctggcag gaaacagc
//
LOCUS       DAWDWH010000021        15480 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_2183_length_15480_cov_18.225219, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000021 DAWDWH010000000
VERSION     DAWDWH010000021.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 15480)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15480)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15480
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2183_length_15480_cov_18.225219"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         4917 a         2838 c         3888 g         3837 t
ORIGIN      
        1 tagagcactt gacttttaat caagttgtcc ggggttcgaa tccccgatgc ctcatgattc
       61 gagaaaagca ttactcatag tgagtagtgc ttttttgttg cttaaaacgg atatacgact
      121 gcgttcatga gcaagtagcg aagcggattg tgaatgtccg ctttacggat attgaaaaag
      181 gaggatggaa atttgaagaa aaatgattta tatatgattt ccggaacgga atataaagag
      241 atgacaaagg agatccttgc acagtcagat ctgtgggcag aaattaaaaa aaaggcagat
      301 cagaagcaaa aagatgcaga tcagatgctg atcggaataa agccgaacct ggtatcgccg
      361 acggaggctt cttatggtgc aacaacacat ccggagattg ttgcggggat tattgaatat
      421 ttacaggaaa gaagttgtca acatatcgtg atgctggaga gctcctgggt tggggataaa
      481 acttcggaat ccatggaagt ctgtggatat gacaggctgg ttgaaaaata taacgtacca
      541 ttttgggata tgcagctcga tgaaagcagt gaagtggatt gtgccggaat gagacttcaa
      601 atctgcagca gtgtgagaaa gctggatttt ttgatcaatg ttccagttct gaaaggacat
      661 tgccagacaa agatcacctg tgcattgaaa aatatgaaag gtctgattcc aaataaggag
      721 aagaggcact ttcataagct gggtctgcat aaaccgattg cacatctgaa tgtggggatt
      781 catcaggatt ttattgtggt ggataatatc tgtggtgatc tggattttga agatggagga
      841 aatccggttg ttatgaaccg gattctgaca gcaaaagatc cggttttatg tgatacgttt
      901 gtatgtcaga tgctttatta taggagagaa gacgttccat atctggttat ggcggaggag
      961 cttggtgtgg gttctgctga tctggaacat gcgaatctga tacagatcag attcgaaaag
     1021 ggaacaaaag aagaagaaca aggatcagaa gaaacggcaa gtggaaaaga tatcaacaga
     1081 aaagcaaaag aagtgaaaga tatccacaaa acagtatgga agggctggga ggatgtggat
     1141 attccaaggg agcgaaagat tgttgaactg caggatgctg tcgaggaagt ggagtcctgc
     1201 agtgcctgtt atggctatct gatcccggca ctggatatgc tgaaacagga aggtttgttt
     1261 gagaaactgg actgtaaaat tgctgttggg cagggatacc gcggtaaaag cgggaaactg
     1321 ggggtcggaa actgtacttg caggtttgag cattttgtaa agggatgtcc gccgacggag
     1381 atagaaattt atgaatattt gaagaaatat ctgatacaat attagaaatt agaaaaatta
     1441 tccaattcat cgaaaaaacc tttgctttag atatggggat ataagatgcg tccattgaat
     1501 ttacgcgtat atcaaatttt gataaaaact tttcattttt tacttgtgaa agtgttgctg
     1561 acatggtatg atattttaag acgttgcatg gagggagcta tgatttaagc ggataaaatt
     1621 gcagctcttc ggtcttgcag tcgcagataa catccatgtg atgtgcgtgg aagtatattg
     1681 tcggattttg gaacttaatg ggaggtatac aacttaattt gctaatttaa gtttgcggcc
     1741 tccctgtttt aaacgagagt ttgttaaaaa atagacaata tcagtaaact tattcaggaa
     1801 aggagtaatt tatgagcagc aaaattacta tcattggagc aggaagtgtt gggtctacaa
     1861 ttgcttatac gttatctaat caggatatcg catctgagat cgttctgatt gatatcaata
     1921 aagaaaaagt agcaggagaa gttatggata ttatccaggg aacctgcttc cgcgacccga
     1981 tcaatatcaa agcaggtgaa tatgaggatg cgaaagattc caatatcgtt atcatcacct
     2041 ccggtattgc aagaaaacca ggtcagacac gtattgagct ggcacagaca aatgtaaata
     2101 ttctcaaaga tatcacaccg aaaattgtta aagaggcacc aaatgctctc tacgtaatcg
     2161 taagcaatcc ggttgatatc atgacttatg tattcacaaa aatttccgga ctgccggaga
     2221 atcaggttgt tggttccggt acgatcctgg atacagcaag acttcgctgt ggactggcag
     2281 agcattttga tattgcacag aagaacatcc acgcttatgt atttggtgag catggtgata
     2341 cttctttcat tccgtggtca cgtgcacata tttcctgtgc aaacattgat gagtacacta
     2401 cactgatgaa caatatcggc aaagaaatcc cgactctgga taaagatgca atgcttgaat
     2461 atgtacagaa atccggtgga aagatcattg caaacaaagg tgctacattc tatgcagtat
     2521 ctgcttctgt atgtcgtctg tgcggacttc tgctgtctgc ttatgattct accgcaacgg
     2581 tatcttctat gatgcacggt gaatatggca ttgatgatgt atgcttaagt acactgagca
     2641 tgatcggtcc gaacggtctg aaaggtaaag ttccggtacc tctgacagac gaagaagttg
     2701 aaaaactgaa gaagagtgca gatgcactga aatctgttat tgcacagatc gaactgtaag
     2761 atttcagcag acaaaacacc atagagaaag aaacttgatg gattttgtac tgcgattgtc
     2821 aaaagactga accgttacaa ataatggtat aaagaatgtt tgaaaaataa cagggaattt
     2881 taaggtatgc agtatgcgat acagaacatt atatccatga aaatcagaat tttcaaaatt
     2941 ctctgttatg tataaagtta ctataaataa aatatcgcaa atatgcggca tcaggaggta
     3001 aaaatggttt acagttattt tcctggatgt actctgaaaa caaaagcgaa agatctggat
     3061 cgttacggca gagaatctgc aaaagccctt ggatttgagc tggaagagat tgaaaactgg
     3121 cagtgctgcg gcggtgtata tccgctggga acagatgaga ttgccagcaa actttcttct
     3181 gttcgtgcat taaatgatgc aaaagaaaaa ggtcaggatc ttgttacgct ttgttcagca
     3241 tgtcatcacg taatcaaacg tgtaaatgat gatatgaaaa atgtggagga catccgcaca
     3301 agagcaaata attatatgca gcttgagcag ccttatgaag gtgagacaaa tgtacttcat
     3361 tatttagagg tactgcgtga cagagtggga tttgatgaat taaagaaaaa agtggtaaaa
     3421 ccgctgacag gaagaaaaat aggtgcctac tatggttgtc tgcttcttcg tccaagctca
     3481 gtactggcat ttgatgaccc ggaaaatcca accattattg aagattttat ccgtgcgatc
     3541 ggagcagaac ctgttattta cggttaccgc aacgagtgct gcggaggata tatttccctg
     3601 aaagaaaaag aaatgagcgg aaaaatggtt gaccgcatca tggattctgc agccggaatg
     3661 ggagcagaag aactgatcac agcctgtccg ctttgcctgt ataatctgaa caccagcaca
     3721 agtgcaaaca agcttccggt tcattacttt acagaactgc ttgcagaggc actgggtctg
     3781 aaagaggagg tgcaggaata atgaaaacag caagagataa tgcagaaaag atcaaagaga
     3841 tcagcggtgc aaatcctcag aaatgtatga aatgcggcaa atgttccgca acctgccctg
     3901 cttttaatga aatggatatc aagccacatc agtttgtatc ctatgtagca gaagaaaata
     3961 ttgatgcatt gatgcagtcc gaaactctgt ggaaatgtct ttcctgcttc gcatgtgtag
     4021 aaagatgtcc aagagatgta aaacctggaa aactgattga tgcagcaaga cagatggttg
     4081 tgcgtcagag aggtgcagat tatctgaaag cagaagaaat accagaactt ctggatccgg
     4141 aactgccaca gcagcttctg gtaagtgcgt tcagaagata taagagataa atgagagaaa
     4201 gttggaggtg aactcattgc agagaatagg agtatttgtc tgtcattgcg gaagtaacat
     4261 tgcagctact gtagatgtga aaaaagtagt ggaaatggca aaactggagc ctggagttgt
     4321 ccatgcagaa gattatcagt atatgtgttc tgaagcagga caggcaaaaa tagatgctgc
     4381 aattaaagaa aaaggtctga caggaatggt agtatgttcc tgttcaccac gtatgcacga
     4441 aacaacattc agaaaggctg ctgaaagagc cggactgaac ccatacatgg tagaaattgc
     4501 aaacatccgt gagcattgtt cctggatcca taaggatatg gaagaagcta caaaaaaagc
     4561 ggttatcctt atgagagcag ccgttgcaaa agtaaactta aatgcaccgc ttcagccggg
     4621 tgagagccgt gtaacaaaac gtgcactggt tatcggcggc ggtatcgcag gtattcagac
     4681 agcgattgat atcgcagatg ccggatatga agtagatatc gttgagaaag agccgagtat
     4741 cggaggaaga atgtcccaga ttgataagac attcccgacg ctggactgtt ctgcatgtat
     4801 cctgacaccg aaaatggtgg aagcttccgc acatgaaaag atcacgctgt atacatatag
     4861 tgaagtagaa aaagtaagcg gattcgtagg tgattttacg gttgatatcc gtaaaaaagc
     4921 aagaagcgta gatatggata aatgtacagg ctgtggtgtc tgccaggaaa aatgtccttc
     4981 caaaaagact ccaagtgaat ttaaccgtgg actgagcaac agaagtgcga tctacacacc
     5041 gtttgcacag gcaatcccga atgtaccggt tattgacaga gaacattgta tcaaattcaa
     5101 gaccggaaaa tgtggtgtct gctcaaaggt atgtcaggca ggtgcgattg attacgatca
     5161 gaaagacgag atcatcacac agaaatatgg tgcaattgtt gtagcaacag gttttgatat
     5221 gatcaaactg gataaatatg atgaatatgc atacaatcag agcccggatg ttattacatc
     5281 tctggaactg gagcgtatca tgaatgcagc aggtccgaca aagggacatc tggagcgtat
     5341 gtcagaccat aaagcaccaa agaaaatggt atttgtacag tgtgtcggat caagatgttc
     5401 tgatgaaaga ggaaagagct actgctctaa aatctgctgt atgtatactg caaaacatgc
     5461 aatgctgatc cgtgataaat atccggatgt agatgttacc gtattctata ttgatgttcg
     5521 tacaccaggt aagaactttg acgagttcta tcgccgtgcg gtagaagaat atggcgttca
     5581 ctacatcaaa ggccaggtag gaaaggttgt tccgcaggaa aacggacagc tgcttgtaca
     5641 ggcttctgac cttctggaca acaaacagat cctgatggaa gctgacatgg ttgttctggc
     5701 agcagcaatc gagccgaacc cggatgtaag aaagatcgct accatgctga cagcaagtat
     5761 cgatacaaac aacttcctga cagaagcaca cgcaaaactg cgtccggtag aatctccgac
     5821 agcaggtatc ttcctgtccg gtgtatgtca gggaccaaaa gatattccgg agacagtatc
     5881 tcaggctggt gccgcagcag taaaagcaat tggtctgctt gcaaaagaca aactgatgac
     5941 caatccatgt actgcaaaac cagatgaact gctgtgtaat ggatgttcta catgtgcaaa
     6001 tgtttgccct tacggtgcaa tcagctatat tgacaaagaa gtaaacgacc acggtatccg
     6061 tgaagtacgc cgtattgccc aggtaaacag tgctctgtgt cagggctgtg gtgcttgtac
     6121 cgttgcctgc ccatccggtt ctatggatct gcagggcttc tctaacagac agatcttagc
     6181 ggaggtagat gcaatatgtc gatagaaaat actgaattca gaccgaaaat tgtagcattc
     6241 tgctgtaact ggtgtagtta tgcaggtgct gaccttgccg gcagcagccg tctggtttat
     6301 ccggccgatg taaagatcat ccgcgtacct tgctcctgca gagtaaaccc gatgtttatc
     6361 ttaagagcat ttgaaaaagg tgcggacggc gttatcatgt gtggatgcca tccgggagac
     6421 tgccattaca gtacaggtaa ttattatgca agaagacgta tgacactgct gttctccatg
     6481 ctggacttta tcggtgtgga aaacggacgt acacgagtag agtgggtgtc tgcggctgaa
     6541 ggtgtgaaat tctctcagac tatgaacgag tttgcagaga agattcattc tcttggtaaa
     6601 aacgtaagat tggaggattt gagatgcagg aagaattgat caaacgtgcg aaagaactgt
     6661 tagcagacgg taccgtggca cgcgtgcttg gctggaaagc cggcgatctg ccttataatc
     6721 cggaaccatc ttattttgaa aacgaagacc agctgaagaa ttttgtatac aatggatttt
     6781 gcggtgcaaa cctgagcaaa tatatgatcg aagcttccaa gctggaagga aaaacactgg
     6841 tattcttaaa accatgtgat acatacagct ttaaccagct gatcaaagaa catcgtgtag
     6901 acagagaaaa agcttttatc atcggtgttg gatgtaaagg taaactgtcc atcgaaaaga
     6961 tcagagagca gggcatcaaa ggtatcgaaa gcattacagg tgccgagatg acagatgatg
     7021 cagaaactct gacgatccag acaatttatg gcgaaaagac ctgcacttat gcatctgcaa
     7081 tgctgggacg ctgccatgtc tgcaaaggaa aagagcatca ggtatacgat gagctgatcg
     7141 gcgaatcaaa agataccaaa gatgcagatc gttttgcaga agtagagaag attgaagcaa
     7201 tgtctccgga agagagattt gcattcttcc agaacgaatt atcaaaatgt atccgctgta
     7261 atgcctgccg taacgtatgt ccggcatgca gctgccgtaa atgtgtgttt gacagcacaa
     7321 aattcgacag tgcacagaag gcaaatgtgg atgatttcga agagaagatg ttccacatta
     7381 tccgtgcatt ccacgtagca ggacgttgta cggactgtgg cgagtgcagc cgtgtatgtc
     7441 cgcagggtat tccgctgcat ctgttcaacc gtaaatttat caaagatatt gacaaattct
     7501 acggagaata tcaggcagga gaggatacag attcgaaagc accactgaca aactttacat
     7561 ttgacgatgt agagccaagc attgttggag aaaggggata atgtatgtac aagatagcga
     7621 aaagtaattt gccggcatta ttccgtgcaa tcgcagaaac aaaagaactg tatctgccga
     7681 tcagaagagc aggacaggta aatttcggac cttggagcga agacgcagaa gttgacgttg
     7741 aaactttaaa gaccgtaaaa tctccaaaag atgtattctt tccacagagc gaaaatctgt
     7801 atagctgtga gagagaaggc aaagatatca agatcgagcc tcaggcatta caggatcagg
     7861 agtttgttgt atttggcatg aaagcctgcg acatcaaagg tgtagaagta ctggataaag
     7921 tatttttagc tgatccgatc gatacatttt atgctgcaag aagagatcac ggaatcatcg
     7981 ttgccatggc atgtcatgaa ccggaagaaa gctgtttctg taaggtattt ggcgtagact
     8041 gtgcagatcc ggaaaaatca gcggcagacg ttgctgtatg gatgctgggc gatgacctga
     8101 tctggaaagc aatcacagaa aaaggtgagg cactgacaaa agcagtggaa agcctgcttg
     8161 aaaatgcaga tgccgacagc gacaagctgg aagaagaaaa gaacaacatc cgtgcgatcg
     8221 tagaaaaact tccgtatagc aatctttccc tggaaggatg gggcggagac aaactggaag
     8281 agaaattcaa ttctccgaaa tgggaagagc tgtacaaacc atgtcttgca tgtggaacct
     8341 gtacttttgt atgcccgacc tgccagtgct acgatatcaa ggattatgat acaggacacg
     8401 gtgttcagag ataccgttgc tgggattcct gcatgtactc tgatttcaca atgatggctc
     8461 acggaaacaa ccgtacaagc cagatgcagc gtttccgtca gagattcatg cacaagctgg
     8521 tttatttccc ggcaaacaat gatggcatgt attcctgcgt tggatgtgga cgttgtgtgg
     8581 agaaatgccc gtcctcttta aatatcgtta aagttatcaa aagcatggga ggtgaacagt
     8641 aatgagcgaa tgtacctgtc ataaaaatta tacactggat acactgattc cgaaggttgg
     8701 tgtgatcaca aagatcacgc aggaaacacc ggatgtaaaa acattctgtg taactgctcc
     8761 gggcggcgga aaactgttcg aacatatgcc gggacagtgt gcaatggtat gtgtaccggg
     8821 tgtcggagag gcaatgttct ccattacttc atcccctaca aataaagaat atcaggaatt
     8881 cagtattaaa aaatgtggtg tactgacaga ctgtctgcac agcctggaag ttggcgatga
     8941 gattactgtc cgtggacctt acggaaataa ttttccggta gatacagagc tgaaaggcaa
     9001 aaatctgctg tttatcgccg gtggtatcgg acttgcacca ctgcgttccg ttatcaacta
     9061 tgtactggat aatcgtgatg actacggaac ggttgacatc ctgtacggat cacgttccgc
     9121 agatgacctc gtaaaactga aagagatcca ggaagtatgg gcaaagactc cgggtgtcaa
     9181 tgtacatctg acgatcgacc gtccgcagga aggctgggac ggccatgtgg gatttgttcc
     9241 gacttacttg aaagaaatcg gtttttcaac agacaagacc gctctggtct gcggaccgcc
     9301 aatcatgatc aagtttgtac ttgcaggact ggaagagctg ggcttcaacc gttctcagtg
     9361 ctatacaaca ctggaacttc gtatgaaatg tggtatcggt aaatgcggac gctgcaacat
     9421 cggttccaaa tatgtctgca aagacggtcc ggtattccgc tgcgatgaag tagacgagtt
     9481 gccgaacgag tactaagaaa aggatcacta gacaaagtac agaaaggaat ggtacgacaa
     9541 aatgcaggaa atggtaaatg tatatttttt cggtaaaaaa tacagtgttc cggcagattt
     9601 aacaatcatg acagctatgg aatatgcagg ctacaccctg aaaagaggat gtggctgccg
     9661 tcatggattc tgtggagcat gtgcaactat ttatagaatc aaaggacaga acgaactgaa
     9721 aacatgtctt gcctgtcaga cacaggttca ggaaggtatg tatgtagcaa gcattccatt
     9781 cttcccgaca gacaagagaa cttacgacat cgaagagatc agaccgaccg agcagatcat
     9841 gatggaactg tatccggaaa tctacagctg tatcggatgt aatgcatgta caaaagcatg
     9901 tacacagaac ctgaatgtta tgcagtatat tgcatacgct cagagaggcg aatttgaaaa
     9961 atgtgcagaa gaatccttcg actgtgtaag ctgcggatgt tgttctgtaa gatgcccggc
    10021 tggtatttct catccgatgg taggtcttct ggcaagacgt ctgacaggaa aatatattgc
    10081 accggaagca aaacatctta ccgagagagt ggaagagatc cacaaaggtg cttatgatga
    10141 actgatcgaa cagatcatgc agaaaccgat cacagagatg caggaacttt acaacaacag
    10201 agaaattgag aaataggagg gcgtaaaata tgtatgcacc atatccggaa aatatgatgg
    10261 agtctatcaa aaaggtagag gctacaagag cggagcgtat ggctacagag ccgagacgtc
    10321 tgacagcaga cgaaaaagac gctcttttga aaaaattcca tccagattat aacccagatg
    10381 cgtttgctga aatcaaggta ggagcaaaca aaggccagaa agctcctctg cagctggcag
    10441 aaatgcttca ttctacaagc cgtctgataa aagataaagt ggatattaca aaagtagatt
    10501 atgacgtaga tgtactggtt atcggcggag gcggtgcagg ttcttcctgt gctatcgaag
    10561 caaacaaagc aggtgccaac gtaatgatcg ttaccaaact gcgtatcggt gatgcaaaca
    10621 ccatgatggc tgagggtggt attcaggcag cagataaaga aaatgactca ccggtacagc
    10681 attatctgga ctgctttggc ggcggacatt ttgctgcaaa accagaactt gtaaagagac
    10741 tggtaatgga agctccggat gcaattaaat ggctgaacga gctgggtgtt gaattcgata
    10801 aagatgcaga cggaagaatg gtaactacac acggcggcgg aacttccaga aagagaatgc
    10861 atgctgcaaa ggactattcc ggagcagaga tcatgcgtac gctgcgtgac gaagtgatca
    10921 accgtggaat tccggttgta gaatttactt ctgctgtaga actgatcaaa gatgacaaag
    10981 gacaggttgc aggtgctgtt cttctgaaca tggagacagg agattactct gttgcaagag
    11041 caaaaaccgt tgttatcgca acaggtggtg caggaagaat gcattatcag ggattcccga
    11101 catccaacca ttatggtgca acagccgatg gtctgatcct gggataccgt gcaggtgctt
    11161 ccctgttata tcaggattct attcagtatc atccgacagg agctatttat ccgtcacaga
    11221 tcctgggtgc tctggtaaca gaaaaagttc gttccgtagg tgcacagctg gttaatgcaa
    11281 acggagaagc ttacatccat ccgctggaaa ctcgtgatgt caacgcttcc ggtgttatcc
    11341 gtgagtgcag cgaaggacgt ggcgtagaag ttccgggcgg agcaaaaggt gtatggctgg
    11401 atactccgat gatcgagatc ctcggcggtg agggaaccat cgaaaagaga atcccggcta
    11461 tgttccgtat gtacatgaac tatggcattg atatgagaaa agtaccgatc gtgatctacc
    11521 cgactcttca ttatcagaac ggtggactgg agatcacagg agacggattt acaaaagaaa
    11581 tcccgaacct tctggttgca ggagaggcag caggcggaat ccacggaacc aaccgactga
    11641 tgggtaactc tctgctcgat gtcatcgtat tcggcagaaa tgcaggtaaa aaagcagctg
    11701 caaaatacaa agacgtagaa cttggtgaga tgaacctgga tcatgtaaaa gcatacgatg
    11761 aagaactgaa agaagcagga gtagaaacaa acgaagtatc tccgatgctg cttcctcatt
    11821 atgcaagaca tgaaagaaaa atcacaggtg aagtggtaag tgcataatta agtaaaaaga
    11881 tgctataaaa ccactcttgt gcaagcacat cgtggtttca ggcttttgat cctggcatca
    11941 ttatattttc atttacggga gctgtcatga gcagctcctg tagataatga gaggagaaat
    12001 aacatgcgtg aaattcaggc aagccagatt acagatgtag tcgaaaagct ctgtatcgaa
    12061 gcaaatgagc atcttccgga agatgtgaaa tgtgcgatca aaaactgcag agcgtgcgaa
    12121 gactgggaaa ttgcccaggg agtactggac aagatcattg agaattatga aatcgctgat
    12181 acacagtgcg taccgatctg tcaggatacc ggtatggcat gtgtattcct ggagattggt
    12241 caggatgtac atatcacagg cggagatctt gcagatgcga tcaacgaagg tgtacgccgc
    12301 ggatatgaca aaggttatct gagaaaatcc gttgtaaaag atcctgtccg cagaggaaat
    12361 acaggtgata atacaccggc tatgatctac acagaaatcg ttccgggcga gcagatcaag
    12421 atcacagtag gaccaaaagg tttcggaagt gaaaacatga gtgcaattcg catgttcaaa
    12481 ccatctgccg gacttcaggg aatcaaagat tttatccttg agacggtaga aacagcaggt
    12541 ccgaatccat gccctcctat ggttgtaggt gttggtatcg gaggaacttt tgataaagca
    12601 gcactgctgg ctaaaaaagc actgatgcgt ccgatcgatt ctgaaaatcc agatccgttc
    12661 tatgcagacc ttgaaaaaga aatgctggaa aaagtaaaca aactgggtat tggaccgcag
    12721 ggatttggcg gaaagacaac agcaattggt ctgaacattg aaaccatggc tacacatatc
    12781 gcaggtatgc catgtgcgat caatattaac tgccatgtaa cacgccataa aacggaggtg
    12841 atttaaatgg aaaaacatat tacactgcct cttacagaag aactggcaaa aacactccat
    12901 gcaggtgata cagtttacct gacaggaacc atttatacat cacgagatgc aggacataaa
    12961 cgtatgtgtg aagcactggc aagaggagaa aagattccat ttgatccgac agatgctacg
    13021 atctactatg taggtcctac accggcaaaa ccaggtcagg ttatcgggtc tgcaggtccg
    13081 acaaccagcg gacgtatgga tgcttatgct ccgactatga tgtctgttgg agcaagaggt
    13141 atgatcggta aaggtgcaag acttccggaa gttgtagaag ctatgaagaa ataccacggt
    13201 gtttactttg gtgcaatcgg aggagcaggt gcacttctgg caaaatgtat caagaaagca
    13261 gagctgatcg catacgaaga tctgggtgca gaagcactga gaaaactgta tgtagaagat
    13321 atgcctttat ttgttatcat cgactgcgaa ggaaataacc tgtacgatga aggacgtaaa
    13381 gcttatctgg atacacagga taaataaaag ttacggcgga ggtttgtgaa tgtgcaaatg
    13441 aacgctgtga gaataggtgt atgaaatagg acgccataat gattcgaaaa caatctcctg
    13501 ctaccgtcag tacgatttgt tttcgaattc attatggctg tattgtttgc aagctatttt
    13561 tacaataccg ttagttgcac attacccaaa gctcctggcc tgccaggggt cgctgaagcg
    13621 gaaggtttta aaaaaatata agattatata atgccagagg tactatgacg gagacttgct
    13681 gttgtcactg tgcctctggt ttttgttttt cagcacaata tacattaaaa agaattgctt
    13741 gattttgtgg aaaaatgtat taagatagag ataactattc ggcagcttta cagttgcgat
    13801 aaggtttgag ctgtatgaag ttattacaga cagagataca agaactgaaa caaaaagacg
    13861 aaagaaaagg agggcagacg agatgggaga gttatttgac aaactggcaa actatggaaa
    13921 ttccggtatc tatccgtttc atatgccggg gcataaaaga cagaaaacgg ttgattttaa
    13981 tccatataaa attgacatca cagagataga agggtttgac aatctgcatc atgccgaggg
    14041 tgttctcttg gaggcacaga aaaaagcgga aaaactttat ggttcggaag aatcgcattt
    14101 tctgatcaac ggcagtacgg ctggcatctt aagtgctgtt tctgcatgtg cgaaaaagac
    14161 cgttctgatt gcacgtaact gccacaaagc agtttaccat gcagcactga tcagaaatct
    14221 ggatgtatat tatgtttacc cggaaattca ggaagaattt atgttaaatg gcggtatcaa
    14281 tccagcagat gtggagcgtt ctcttgagga gcatccggaa atcgaggcgg tcgtgattac
    14341 ttcgccaact tatgatggca ttgtttcgga tgtgaagaga attgcagaaa ttgcacatgc
    14401 acatgaaaaa ccattgatcg tggatgaagc tcatggagca catttcggat tttccaaata
    14461 tttcccggaa aattccgtgc atcttggggc agatatcgtg atccacagtc ttcataaaac
    14521 gctgccttct tatacacaga cagcactgat ccatatcaac ggaaaactcg ttgaccgtca
    14581 gaaagtccgg atgtttctgc agatgtacca gaccagcagc ccgtcctata ttctgatggc
    14641 tggaatagat gaatgcattc gttatgtgga agagcaggga acagatgctt ttgataaatt
    14701 tgctgagagg ctggatcgca tttataaaag ggcaaaagaa tttcgttcca ttcatttggt
    14761 ggatgagagc attgtcggaa aaaacagtat ttatgatttc gatcgttcga agctgatttt
    14821 ttctgtaaaa aaatgcggaa tcgccggaaa tgaacttcag aaaatgctgc ttcgtactta
    14881 caatatcgaa atggaaatgg cagcgggaaa ttatgtactg gcactgacct cgctgtgtga
    14941 cacggatcag ggctttcaca gactgtttta tgccgtcaaa ggtattgatg aacagatcaa
    15001 ccgtttccag actacagaaa gcaaaaaaga aggtaactgg attgaagatg gagttacaag
    15061 aaatactatc atttgcacga tgaataaagc atttgaatcc cagaaagaaa gcgtggcttt
    15121 gcaggaaagt gagggacgga tttccgggga atttctgtat ctttatccac ctggaatccc
    15181 gatgatcgtg ccgggagaga agatagatgg cacacttctg aaaaaactgg aagggtataa
    15241 gaagaaaggc tttcagttcc aggggttgag ggattatgga ggagagaaaa ttgaagtggt
    15301 gaagtaaata aaaattgacc gagctcttta gagcgaggga tgcttcttgt ccgaagggca
    15361 agaagatggg tattaagaaa acttatacgt aaaatataac gccatgatga ttcgaaaaca
    15421 atcttctgac acgctgcgtt tcctgttcgg ttcgctcgcc tgttagccgc ccttcgccta
//
LOCUS       DAWDWH010000022        15149 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_2239_length_15149_cov_10.804691, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000022 DAWDWH010000000
VERSION     DAWDWH010000022.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 15149)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15149)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15149
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2239_length_15149_cov_10.804691"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    450..459
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2215..2246
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3772..3781
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10790..10799
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4424 a         2851 c         3873 g         3939 t
ORIGIN      
        1 ctggaatgta ccagcagtgc actcaccaag gcaaactggg agtgtgaacc ggcatccctg
       61 aagatcaaga ttgaaaatgt tgtgcaggaa tcgttcggtg tggaagtgga tacgaccggg
      121 gagccggtcg atggatatga catcgggact tctacgatca aagagggcga tacgatcaat
      181 attgcggggg cggagtcgct gatgaatatc attcacagag ttagtatgtc ggtgagcgtg
      241 aacggacttt cttcggataa aacagtaaaa ggaaatattg ttgtcaccga taaaaatggc
      301 accgatttta cacagagcca gttggataag ctggtcttta caacgagcag cggcgacctg
      361 atcaaagatg gcgtgttaag tgcaaagatc cagctctgga aagtagaaca gaatgtaaaa
      421 gtaaatatcg gaacttacgg tgacccggcn nnnnnnnnnt gttattatgg agaagatggg
      481 aaccaagaaa attgatattc ctctctctgc gattacgata aaaggaagac cgaccgatct
      541 ggatatggta ttgacacctg cagataagat cacggtagaa gttgcagcag tagacggatc
      601 gttggataat attgatcaga atgatgttaa ggcagtgttg gatctgactt cttatcagaa
      661 agaagggaga catgaagttc cggttcagat taccctgccg aatggctacg aactgacttc
      721 ggaagtgaag attgtcgtaa acctgaccaa aaagcagaag acaagcggca gtaatacggt
      781 gatttcagct gcggaggagt agaagtgagt aaaatttatt acagggaaaa ccgcagaatg
      841 cggtaacagg agggtaaact tatggtaagt cagaaggtaa gagtgaaaaa tccttccggt
      901 cttcatttac gtccggcagg agtactttgc agtacagcgt tgcagtttaa gtgccgcgtc
      961 agatttacat ttcgtgaagc gactgtgaat gcaaagagcg tactcagtgt gctgggagcc
     1021 tgcgtaaagg aaggtgatga gatcacgttc gaatgtgaag gggaagacga ggatcaggca
     1081 cttgccacga tggtaaaggc tgtggaagaa ggacttggag aagaacagaa aaattaagat
     1141 cttcaggaaa cgtagatgaa aatctgcccg gttgttttga atgaccgggc gggtttttgc
     1201 ttttctgtat tctcttgttt acgtcaaaga tctattttcc ggtgttaaag tacagtggat
     1261 cagtagggtc atagctctga gagagtggga ttttaatgcc ttttggttta ccgagcggtc
     1321 ccgggtttgg agaatcgtat actacaacgg tcgtaccgat cggaacggtg ttgaacagcc
     1381 agtaggcatc tcccatactc aggcgaatgc agccttcaga agcctgagtg ccgagtaggt
     1441 tgtatttata ggccggcaga ttttttctgc caagctgtgg ctgtgggatc gaatgaaaca
     1501 ggatttcact tgtgatgtga gaacagtaat atccaaacga aggtccataa agtgcatggg
     1561 tatacagttt atcctggatc gtgaatgtac cgagcggagt tccgttgttc agaccggttg
     1621 agcatatcat ggcacgcacc ggagtattgc ctttgtagac agtaactaca ttttgctgtc
     1681 ggtttacttt tacggtccag ttcccttcca gagcacggct tgagattccg gaatctgaga
     1741 atgtatagga ggtgccgccg atatttttag tgccggtcac cataacaccg tcggacggat
     1801 tcagataata gtacttacca ttatagtaca gccatccggt atgcatggag ccgtaatcat
     1861 tgaaaaagta cgtttttccg tcaatcgttt taaagcctct ggtgatttcg ccctttgcgt
     1921 ttgcataata tttgcggctg ccatagacgg tccagccttt tccgagaaca gcgcttgaat
     1981 caaaaacata gattttcttt ttgatctgct gtacgccgat gtaaggtacg cccatattat
     2041 ttcccgaagc atcggcataa tattttttgc ctttagcttt gaaccatccg gtctgaaggg
     2101 tgccgtcttt tccgacgcga cactttttcc catctttttt tacccatttg tttttataca
     2161 tggctcctgt ttttttgttc aggcagtacc atttgttttt ataaaggaac catcnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnngtac ggtttgtaaa cagctgataa ttggctccgg
     2281 cataatacca ggagatacca ctttctgatt ttatggagaa aagacctgtt tttacagatc
     2341 cgtccgcaag gaaataataa ggtttttggg cgaggtacat gacaccttcc tgggatggcg
     2401 taaaaagata gccgtccggg tcaaaataat atactttctg atcaatgctg caaaatcctg
     2461 tggcacggga accgttctct gtcagaaaga aacgccccag ggcatctgaa ttccagccat
     2521 atgtgacagg tgtttccgtt ttcgcatctg gtgcagcata aatagcctgc gggatacaga
     2581 aaatcagggc agatgccaga atgctggcag acaatatttt cttaaaatgc ataaactcct
     2641 ccattctctg gaaatttgca gtcaaataaa acatggatta taaaagataa ttctgttacc
     2701 attactttac tataaaatga tgcaagtgtc aaaagtctaa attgcgatca ggcttgtaca
     2761 aatgagcaat ctagacttat tgacacaccg aacacgtaat aaaatgtcgt gtatgcggca
     2821 ttttatggga gtttgaggtg gttccggtac aatccgaaac aaaaattaaa taaaaattaa
     2881 gaaaaattaa cagaggttta agagattgac aggcagacct ttttagggta ctatgtaaag
     2941 taatgtgtat cacaaaaatg aacgaaaata agaagtgatt tttacattgt ggttgtaaaa
     3001 ataagaagaa attacgagag tatttcgaaa tcaggaggaa aagatgaaga aaatattaaa
     3061 aaatctggca gcagctgcga tgctgggact tgtgttctgc acgggggtca catcacatgc
     3121 ttcgaatgga aatgttgttc tggtactgga tcccggacat gatgtacacg atgcaggtgc
     3181 gagacgtacc tggaacggtg tgacttatgc ggaggagacc attactttaa aaatggcaca
     3241 gtactgtaaa gaagagctgg aaaaatactc cggtatcgtt gtttatatga caaggttcag
     3301 caataatgtg gacatggatc gctatgaccg tgtaaaagtg gcaaaagatc tgggagcaga
     3361 tgcactggta agcctgcaca tcaattcaac cggaaatcag caggataccg tttccggagc
     3421 acttgcctat gtgccaatct caaccaacaa agcagattat gctgtggaag caagagcact
     3481 tgctgcggat attgtgagta atctttcaac ggtcggaatg aagaatctcg gctatctgaa
     3541 gggggaagga cttggcatta tctattatgg aaggcagtgg aagatcccga ccatgatcat
     3601 agagcatgga tttgtgaaca atccttctga ctgtctgaaa tattatggaa gcgatgccaa
     3661 gatcaaagcg gtggctgttg cagatgcaac tgcgatcgca cgccattacg gtatcacgaa
     3721 aatgcgtggc tggaatcaga tcggagatga atggatttat ctggataaga annnnnnnnn
     3781 nattaccgat gcaggcaaag aatattatct ggacgaagaa gggcataaag tttccggatt
     3841 tgtaaaaatt aatgataaaa aatattattt taatgaagac ggaagtgcct acagcggatt
     3901 ccttcaggca aacggtgccc tgtactatgt gaaaaacggc ggtcaggtga tgacgggccg
     3961 gtttaaaata ggcgagaaac agtactatgc ggcaaagggc ggcaaactgt atcgtggatt
     4021 taaggtacgc aaaggttaca aatactattt tgatccggaa accggagcag cacttagcgg
     4081 attgcagaag atcggagcaa actattatta ttttgatgtg gcaggacgta tgcagaccgg
     4141 ctgggtaaaa gtcggttcca gaacctgtta ttttaaaaag tctaccggtg tgagaagaag
     4201 cggatggttc aaatacagag gcaaatatta ttatctgaat ccgaagaccg gagccaaaat
     4261 gaaaaaacgc tgggtagtat ataatggaaa ctattattat ctggacagga aaggtgtccg
     4321 ccttaccggc actaaggcag tgatcgacgg caaaaagtat cgttttgatg caaatggcat
     4381 ctgcaaaaag aagacaagag ccgaggcagc cagacagaag gcagaacagg tgacaccgga
     4441 aacaggtaat gtgacatcga caacagaaca ggtgacaccg gaaacgaaag cactttcgga
     4501 ataaaggaga aaaagtcgga tggcgatcaa aggattcgag attctgctgt ggtttcttat
     4561 cattccgctt gcagcaggaa atcttcctgt ttttgagaca gggaaggaga aagactggtt
     4621 tgtgcgaatg gcagatgctc tgatctgcgg ttatgttctg ctgtttgcag tatttgagct
     4681 gctggcgttg ccgctcattt ttaccaggca gtcatttgca gttctgaaat acagctatga
     4741 gatacttgcc tgcgtgctgg ctctggcagg agtcatcttt gcctggaaaa ataaaaagaa
     4801 cagggcagat ggagcagaac ggaaaaagag cctttcacga aaaaaaattc cagcggccat
     4861 gtggcttgca tttctgctgg tcgccataca gatgggagct tatgtttttg gcatggcaac
     4921 ggatctggat gacgcctttt atgtggcaac ggcaaccacg actctggaga cgaacgggat
     4981 gttcacgtac gatgcctata ccggaatgtt ggcatcgtat cttccggcga gatatgtgtt
     5041 tgcaccgttt ccgatcctgc ttgcattcta cagtgatatg gtgcatatgc atgcggcggt
     5101 tgtggcacat acggtcgaac cggtcttctt tcttctgatt tcctatcttg tttactggaa
     5161 gatcggaaga aaactgtttg ataaagatga tcggaaagtg ggactgtttc tgctgtttct
     5221 ggtgctgatt cagatgtttt cttattattc agtctacacg cagggaacat ttctgtcgat
     5281 ccgtatctgg cagggaaagg ctcttctggc atcgtttgtg cttccggcga tttttttgca
     5341 ggcaaaggaa tgcatggaaa cgaaccggat gtgcggtgca tgggtgacat tgtttttgat
     5401 gatgacatcg gcatgtctag tgtccggtat gggtattatg ctggcaccga tcatgctggg
     5461 gcttatgaca cttttgtatg cggtgaaaga cagaaattgg ggaaacataa aaagagcagt
     5521 gatatgctgc ctgccgaatg ttatctgtgc ggcggcatat gtgatcatac gctagaagaa
     5581 agggtagaat catgataaca gcatttcaga ttgccataga aagggcaggc tggtacatcg
     5641 gaagtggatg gcattatctg ttgtttgcag cggcactcct ctttctgatc ctgaaacgtg
     5701 atgacaaaga aaaccgtaaa tggctggtgg ggtataccct gttatttgca gccgtttata
     5761 tctgtccgct tacggcaagg atcattatga aatactgcat tggtggattt gtttactgga
     5821 gaatgttctg gattctgcca acgtccgtga ttttagccta tgtggcagtg agcgtatgta
     5881 cagcaggaaa gaaaaaaacg attcaggctg tatgtgcatc tctgctgatg gcattgatca
     5941 tcgtgaccgg aaagaatcct tatgtgggcg gtcaggccat ctatcagaaa gcagtcaata
     6001 tgcagaaact tccggcggat gcgtgtcaga tcagtgaact gatagccgcc acaagagcag
     6061 aaggcgagac ggctcttgca gtgatgccgg aagatctggt cggttatgtg cggcagtatg
     6121 atgcatcgat ccggctgctt tacggaaggc gttccaaatc agagaaacca gtgcggaaaa
     6181 tccgccgtca gatgcgaaaa gaacagccga agatcaaaaa gctgatccgc aggatccggc
     6241 agcagggcgt gaattatctg gtttttcttg cagatgatca gcaggatact aaaattcaga
     6301 gaaatggatt tgaaaagata ggaaaagtag ggggttactc ggtctataaa gataaatggt
     6361 aagcataatc ccatgcattg ccacagactt tgctattgta attttggaga aagtgttgta
     6421 aacttatcgg cgatgtaaaa gtatagaatt ttatatgaag aggataagta tatggaaaac
     6481 agagaacgtt ataaaagact gatcatgttt ctggcatctg catttattct gacactccag
     6541 acaggcgtgt tcgcttatgt atggtttaag gattatgcat tttccggagc gatcggagaa
     6601 gctttctgga aacgcggaaa ctatgtagtc attgcacaat atgctttgat gctgtacttc
     6661 ttctaccgta tttacggcgg cttcaaagta ggatatttgc gggtttttga agtactgtat
     6721 tcccaggttc tttccgtgct ctgtgtaaat gcgatcacgt atctgcagct ggcactgatc
     6781 ggacgctgga agtttacgaa attcatagag ccgattctga aaatgaccgg cgttgacctg
     6841 gtgatcgttg tagtctgggt cgtatttatg cggtggatct atacaaaaat ctatccgccg
     6901 agacagatgc tgctggtata tggagattac agcccgcagg atctgatcca gaagatttcc
     6961 agccgtgagg ataaatacaa catctgcgag aagatccata tcagcaaagg gctggatgcg
     7021 atcaaagggc ggatcgatga gtatcatgca gtggtacttg gagatattcc ggcatccgag
     7081 cgaaatatcc tgctgaaata ttgctttgaa aaagatatcc gctgctacag cattccgaag
     7141 atatcggata ttatgatcat gagtgcggaa aatattcacc tgtttgatac ctctctgctt
     7201 ttgttccgcc atcgcggact gacagccgag cagcagttct tcaaacgggc gtttgatatt
     7261 gtaacttctt tgcttggaat tattgtggca tcgccgttta tgatcgtcat tgccattctg
     7321 gtgaaactgt acgacaaagg tccggtattt tataaacagg cgagactgac aaaaggaggc
     7381 aaagtctttt atgtaatgaa attccgcagc atgcgtgtgg attcggaaaa acagggagca
     7441 aggctggcga tgaagcatga tgatcgtatc acaccggttg gacgtgtgat ccgaaatctt
     7501 cacctggatg aattaccgca gcttttcaat atcctcaaag gcgatatgtc tatggtcggt
     7561 ccgagaccgg agcgtccgca gatcgcagaa gagtacaaaa aagcaatacc ggagtttgat
     7621 taccgcctaa aggtgaaggc gggactgacc ggatttgcac aggtttatgg aaaatacaac
     7681 acaacaccgt atgataaatt aaaactggat ctgacctaca ttgagaatta cagttttgtg
     7741 ctggatataa aactgatgtt tctgaccttt aagatcctgt tccagaaaga aaataccgaa
     7801 ggcgtggaaa aatggcaggt gacggctgct accaaggaaa atctggagca gatcaaagaa
     7861 gaggaagaaa aggaaatgaa agaaaccgga aaacagcaat aggagaaccg tatttatgga
     7921 aaaaagaccg ttagtatccg tgatcatacc ggcctataat tgtgcagggt tgatatcaag
     7981 agcgatcgag tccgtgttga tccaggaggt atcgctggag atcatcgtta tagacgatgg
     8041 ctccggtgac aacctggagc aggtgctgga gacgtatcgg gataaccctg cgttttcttt
     8101 tattaaaaat aagacaaatc tgggtgcggc cggaagccgg aacaaaggtg tggccatggc
     8161 gaaaggcaga tatgtcgcgt ttctggatgc cgatgattac tgggcagccg ggaaattaaa
     8221 aaagcagaca gatgctatgg aaaggacagg ctgtgtttta agctgtacgg caagagagct
     8281 gatgactcct cagggcgaac tgaccggacg gatcattccg gtaaaagaag aaatcaccta
     8341 ccgtgacctt ttgcatcata acagcatcaa ctgttcttcc gttgttttga aaacggaagt
     8401 tgcgaaaaac tatccgatgg agcacgatga cagccacgaa gattacatta cctggctgaa
     8461 aatcctgcaa aaatataaaa aagcttgtgg tatccgggaa cctctgttaa aatataggct
     8521 aagtaatcag ggaaaatcag gaaataaatt gcagtcggcg agaatgacct ttatggtata
     8581 tcgttacctg ggttttggca tcttgaaatc ctgttattat tttgcttcct atgctctcca
     8641 tggtgtgtgg aaatattatg gattttccgg gaaagagcat aaaaagacaa cggaaaacca
     8701 gaggagatag ggtatgaaaa gttttatcaa atatctgtac agcaaatgtc tgcagatatt
     8761 gctgtttcca ttttgcctct tcccgatcca gaagaaccgg ctggctttta ccgggcttac
     8821 aggcggcaaa ggatatgatt attcctgcaa tccgcgatat ctttcggact atatcagaga
     8881 gcaggagaag gacacgtttg agatatactg gatggttaca gatccaaagc agtatcgtga
     8941 caaagaagag aaggatctgc attttgtgaa acattttacg ctgagaagtt tttattatct
     9001 tctgacggca aaagtgatca tcacgaacgg ctcttatgct ccgtggtttc cgtttcgcaa
     9061 aaagcagtat ctgatcaaca cctggcatgg cggcggagct tataagaaaa ttgaaaatga
     9121 caaaccggat gccaactggg cgaccagaaa gcgtgcagaa ttctgtgcga aaaatataca
     9181 gctgatcgta tcaagctgtc aggcggcaag tgacaggctg tttcgccgtg catttcttta
     9241 ccatggagaa atcttaaaat gcggtatgcc gcgaaatgat tttctggtct gtgggaacac
     9301 agagcaggcg gatcagaaag taaggcagac ctatcagata gaccgtaagt atcggatctt
     9361 gctgtatgca cctacctatc gttcaaatgg gaaaaatcag atgccggatt ttacaggact
     9421 tcgaaaagta cttgaagaaa atggcgaaaa gtggtatgta ttataccggg cacatcgcta
     9481 cagtgaggac gattcggcag gattggatcc ggaatgctca gcggatgtga cggactatcc
     9541 tgatatgcag gagcttttag cggcagcaga tatgctgatc acggactatt cctcctgtat
     9601 atgggattat tcttttttat accgaccatg ctttctgtat acaccggatc tgcgggaata
     9661 tcttgcaaag accggttttt atgtagatat tcatgagtgg ccgtttccaa tctgtgaaaa
     9721 tatggaagat ctggaaaaac agatccgctc tttcgatgaa gaaaagaaca gagcgaggat
     9781 catggagcat catcagcaaa tgggaagcta tgagagcgga catgcctgcg aggcggtctg
     9841 cagttggata aagcaaaaaa gtatttgtga ggagaacgca taaaatgagt cgatttctaa
     9901 aagacacgaa aaagtactac aagtacgcga tctattcggc aaaatcgaaa ttgcagtctg
     9961 aggtggcaag ttcctacctg aactggctgt ggtgggtatt ggacccgatc tgtttcatgc
    10021 tgatctacac cctgatcttt ggtgtggtat ttaatgcaaa agagcagtat tttacagtct
    10081 tcatttttat cggtctgacg atgtgggatt tctttaataa gacccttcag cagagcgtaa
    10141 aaatcgtcaa aaacaacaag tccattgtgg caaaagtata tttgccgaaa tacattctgg
    10201 ttatggtccg catgtttgta aacggcttta aaatgctgat ctcttttggc atcgtgatca
    10261 ttatgatggt catttaccgc gtgccggtca gctttaatat tctgtacatg atcccgatca
    10321 tgctgaccct gtttctgttc acgttcggat gcagtacgat cgtggcacat tttggcgtat
    10381 ttgtacagga cttgtccaac gtgatcaata tcgtactgcg tctgctgttt tatattaccg
    10441 gtatttttta taatgtagaa acaaggcttg caaaatttgc accgtggaat aaagtgatcc
    10501 ttcattgcaa tccactggca ctgcttctga cttctgcaag aagatgtatg atctatgacc
    10561 agacaccggc aagaaaattc ctgcttctgt ggatggtgct gagtttcata ctgtcagcga
    10621 tcggagtaaa gattatttat aaatacgaaa acagttatgt gaaggtgata taatggaacg
    10681 cgaaattgca atcgaagtaa aaaatttata tatcagttat cgaaacctgc agaagttttc
    10741 catcaagcag agtttattac atttcaaaaa agtacaggcc gatgtattcn nnnnnnnnng
    10801 gatgtatcct ttgaagtaga aaaaggagag atcctcggaa tcatcggaaa gaacggaagc
    10861 ggaaaatcca ccatgctcaa agcactggcc ggcatttttt ctccggatac gggaagcatt
    10921 gacctgaaaa accattccgt ttcccttctg tcgatcggtg tcggatttga aaaagagatg
    10981 agcggaagag aaaacatcat tctttccggt atgcttcttg gtttttcgga agagtttatc
    11041 cgttccaaga tggatgagat cattgaattc gccggacttg gcaaatttat tgatatgccg
    11101 gtgcgtactt actcaagcgg tatgtattcc aagctggcat tttccatcac agccgttctg
    11161 gagaccgaca tcatgctgat cgatgaagta ctcagcgttg gtgatgaaaa gtttaaaaag
    11221 aaaagttatg aaaagatgaa gagcctgatc tcaaacagag agcgtacggt tgtgatcgtt
    11281 tcccacagcc tggatacgat cgcagatctc tgcgacaaag tcatgtggat gcatgaagga
    11341 aaactgatcg agatcggaac accggatgag gttcttccgc gttatctgga atttatgaaa
    11401 taggaggcag catcgtggaa aaaacaaata tttacggcgt tgtacttgcg gggggcaagg
    11461 gaacacgtat ggggaatgtt gagaaaccga aacagtttat ggaagttggc ggcaagccga
    11521 tcattatcca tacactggag aaatttgtgg tcaatccgca gtttgagcgt gtgatcgttc
    11581 tgtcaccgaa gcagtggatc acctacaccc aggatatcat taaaaaatat attctgcttt
    11641 cagataagat cgatgtgatc gaaggcggtg cgacccgcaa tgatacgatc atgaatgcga
    11701 tcagtcatat cgaaaaattt tacggactgg atgaagaaac cattattgtc acccacgatt
    11761 ccgttcgtcc gtttgtgaca caccgtattc tggaagataa catccactat gcgaaagagt
    11821 acggagcctg cgataccgtt atcccggcga ccgataccat tgtggagagc atggataatc
    11881 agaagatcag caatattccg gatcgttcca aaatgtatca gggacagaca ccgcagagtt
    11941 ttcatgcaaa gcgtctgcgt gaagttttcg atgaactgag tgaggaagag aagacgatcc
    12001 tgaccgatgc ctgcaagatc ctcgttctca aaggggatgc gatccatctg gtagaaggtg
    12061 aagttttcaa tattaagatt acgtatccgt acgatttgcg tgttgcagag tctattttag
    12121 gaggaaatgg cgtatgctaa atacggtgta tcagctgaaa cgtccaagac agtttgaagt
    12181 cgcatttaag gaaatggaga tcgatgacag acaggtgatc gtgcgtccga ccagactgtc
    12241 tatctgccat gcggatcagc gttactatca aggtgcaagg gcggaagaga ttctgcgtca
    12301 gaaactgcct atggcattga tccacgaggg gatcggcaaa gtaatctatg atccgaccgg
    12361 aacctttgaa atcggaacag aggttgtgat gatcccgaac cgaccggtag aaaaagacga
    12421 tatcattgca gaaaattatc tgcgatccag caaattctgt gcgagcagca tggatggatt
    12481 tctgcaggaa tatgttcagg ctgtgccgga tcgttttgta cgtctgccgc aggggatcaa
    12541 tccgacggta gctgcctata cagagcttgt cagcgtgagc tttcatgcga tcaaccgttt
    12601 cttacgtttt tcacatgaga gacgtgatgt gatcggtgtg tggggcgatg gcaatttggg
    12661 ttacattacc tcactgcttc tgaaaaagat gcttccggat tccaaaatct atattttcgg
    12721 tgtcacggaa aacaaactgt cggattttac ttttgcagac ggtacctact gtgtcaatga
    12781 aattccggca gatatgcaga tcgatcatgc gtttgaatgc gttggaggcg gagcggcatc
    12841 caaagcgatc aatcagatca ttgattatat ccatccggag ggtacgatca gtattttagg
    12901 tgtcagcgaa gatccggcac cgatcaatac ccgcatggtt ctggaaaagg gactgcgtat
    12961 tttcggcagc agccgcagcg gcagaagtga ctacgaaggt ctgctgcagt tatatgaaga
    13021 gcatccggac gtagtatctt atctggcgaa tatcctgggt gcgggagtag aagtaagaga
    13081 tgtgaaggat ataaccagag catttgaaat ggatatacac aaattgatgg gaaaaacggt
    13141 gatgatctgg aatgagtaaa aagataaaga gactctatct ttttctgacc ttaaagcttc
    13201 tttttccctg gcaatacagg agatttcgta agctgccttt aaaaaagtgg aaagcggtgt
    13261 tcattgaagc caatctgaca cagctttccg gcagcttacg ttttttgtat caggtattat
    13321 gcagcgaaga agagatggaa gtgagcgtcc actatctgat ggaatctgga aacaaatgga
    13381 aatttattca gaatgcgatg gcatgtctga aggactgtgc cgatgcacat tatatttttc
    13441 tctgtgaggg ttccagggtg atcagctgca ttgagccaag agaagagacg gtgatcacgc
    13501 agctctggca tggatgcggt gcgttcaaaa aatttggatt cagcacctgt gaggagcttt
    13561 tcggcggaag cagagaagaa tatctgaaat ataattatta taagaactat gactatataa
    13621 ctgtatcaag ctcggaggtt atctgggctt atcaggaggc catgaatctt ccgaaagagg
    13681 acaaaagtgt tgtaccgatc ggtgtcagct gcacagatct tctgttcctg gaaaaatttc
    13741 agaaggcagc acagaaaaaa ctgtatgaca tctgtccggc agcaaaagga aagaaagtca
    13801 tgctctatgc cccgacgttc cgcggaagtg tgggaggagc gaaggcaccg gatgccatgg
    13861 atctctgtct gatgcaggag aagcttggaa aagactggat tttgctgatt aaacagcatc
    13921 cggttgtgaa acagagaccg gaggttccaa aggagtgtca aagctttgcg tatgatgttt
    13981 ctgatgcatt tgtcatcgaa gaactgctct gtgtcagtga tctgtgtatt tcggattatt
    14041 cgtcgctggt atttgaatat tcattatttg agcgtccgat gctgttcttt gcctacgatt
    14101 ttgaagaata tggagagtgg cgtggttttt actatgatta ccatgaactg acaccgggac
    14161 cggttgtgca caccacgcag gagattgttg attatgtact ccatcttaag gagcgttttg
    14221 acagaaaaca ggtcgcggat ttcaaggaga aatttatgag tgcctgcgac ggacatgcaa
    14281 cagagcgact gctggaaatg atcaagaagg agaaaacgga tgagtaaggt aataagagca
    14341 atgaaaaagg tttactggaa aggaagaaag aagattaaga aacttccgcc ggtaaaagca
    14401 gcaaagaaat atatgggcag aaagagaaaa gacaagcttc tctatgagac actgccggct
    14461 gcatacaatg cacataagaa tgagccggtc gatgaacgaa aagtcatttt tattgagctt
    14521 cgtctgccgg acgtaagcaa cagtttccgc attctgtatg atgagctgca aagagatttt
    14581 gacctggata tccatgtaca tctgctgcga aattcttttg tcccaagaga agaatacatc
    14641 agacgctgta aagagatgct ggccgatgcg gcaactgcaa aatacatctt tgtaaatgaa
    14701 gcttccgacg tactcagctg cgtggatatg cggccggaaa cgatcgttac ccagctgtgg
    14761 catgcatgcg gagccttcaa aaagtttggt atgagcactg cggagctgat cttcggaccg
    14821 aatgcagaag agctgaaacg ccatccgaac aacaagaatt atacttatgt taccgtatcc
    14881 agtccggaga tcgtgtgggc atatgcggaa gccatggata tgaaggaccg tcaagatgca
    14941 atccgcccgg tcggaacgag ccgtacggat attttctatc gcagtgagac gatcgctcag
    15001 gcattccaga acctgtacaa agaatttcca aaagccaaag gaacaaagat catcttatat
    15061 gcaccgacat tccgcggtcg tgtggcgagg gcaaaaacgc cggactgctt tgatctggag
    15121 aagtttgccg agcatttcaa agatgaata
//
LOCUS       DAWDWH010000023        15094 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_2246_length_15094_cov_10.596715, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000023 DAWDWH010000000
VERSION     DAWDWH010000023.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 15094)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15094)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15094
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2246_length_15094_cov_10.596715"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2020..2029
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3682..3691
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7063..7072
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9434..9443
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10114..10123
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12202..12211
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13817..13826
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3531 a         4074 c         2933 g         4486 t
ORIGIN      
        1 gcacacccat cactttttat cgaaaatcca ccggtaccgg ccgtaccgaa tgtaatgcag
       61 agggtatcaa acagcggcat tttgctgatc aacagcaaaa tgatctgaat cagcgtcata
      121 ccaaaataaa tagcataaag gattgctgcc gtatagcgga tcttcggcac gagtttgctc
      181 acgctcggtc ccggactttc tgctttcatc aggtgtacga ttgttccatt tcctttattt
      241 ggaataaaag caaggataaa gaccagaatt cccatacctc ctacccagtg ggtaaaactt
      301 ctccagaaca gactggcacg tgaaagtgcc tctacattgg tcagaatact ggctcctgta
      361 gttgtaaaac cggagctcac ctcaaaaaat gcatcgaaaa aattcgggat ttctcctgtc
      421 agcataaacg ggactgcccc aaatacagaa aaccagatcc atacaagtgc aactgctacc
      481 agaccttctt tggtatacag agtaatcttt ttcggcttgc gaagtgtgct gaccccgcag
      541 aataatgcac agatcccgca ggagatcaga taagaagaga gaacactttc cccgtagatc
      601 agcgaaacca ggatcggaag cagcagaaag agtgcttcga acccgctcgc catgccaagt
      661 atgtaaaaaa tcattccata attcatgttt tcacccttac cgttctttca caatatcgga
      721 tatatcatcc agtccctgat tggtcgtgac aacgatcacc ttatccccga tgctcatcac
      781 gtcctgaccg ttcggcatga tcagcacacc gtcacggtaa atcgatgcga tcagcagatt
      841 ttttttcaga gacagtttct caagcggaat gtcaaggatc ggagacgact gccggatcac
      901 aaattccagt gcttccacac ggttgtttac caggcggtaa agtgtctcta cattactgcc
      961 gatggtattt ttcatggcac gtacataacg cacgatgtat tctgccgtca catacttcgg
     1021 ataaatgata ctgtcaagat ccagtttgtt gatgacttca tcgaacgtgg tattgtttac
     1081 ttttgtgata ctcttgatct tcggtgcatg ctcttttgca aagagtgaga gcaggacatt
     1141 ttcttcgtcc acatccgtca gcgtcacaaa ggagtccatc cagttcacgc cttcttccat
     1201 gaggacatcc tgatccgttc cctcaccgca gatgatcgtc gccttcggca gaaggtcgct
     1261 taattcttcg catcgctcac ggtttttgtc gatgatcttg acatcaatgc ccatggtcag
     1321 aagctgtttt gccagataat aggctgttgt actgccgcct atgatcaggg tatcacgaac
     1381 ctgatccgtc tggactttga tctttttaaa gaactggctc gccattttcg gcggtgccac
     1441 gatcgacacg atatcacgct cctgcagcac gacatttcca tccggaatga taacctgatc
     1501 tccgcggaca acggtgcata ccagcacttc gcagtgaagc tttgccccca tatctgcgat
     1561 ccgcatgtta ttcaaaacag agtttgcccc gatacggaac tgcaaaagct caatgtgtcc
     1621 gtttgcaaaa gtctcgatct tgatggcaga tgggaaacgt aagatccttg ccatctcgga
     1681 agctgccgcc tgttccggat tgatgaccat cgccatacca agctcttccc ggataaagcg
     1741 gacttcctga ctgtactccg gattccgcac gcgggcgatc gtctgacagc cgtttccggc
     1801 ttttttggcg atcagacagc acagcaggtt cagctcatct gaaccagtca cggcgatcag
     1861 cagatttgcc tcccggatgc ctgcctccag ctggacatta tggctgcttc cgcttcctac
     1921 cacgcccata atatcacacg cgtttgatat atcctgcact ttttcggcat ctttatctac
     1981 aataacaata tcgtttcctt cattactcaa ctgttctgcn nnnnnnnnna tgatcttcat
     2041 ttctttttcc tcaccttgaa ttttctgttt ttcgatttcc tttctctgtc caaatacagg
     2101 taagccgaaa acggccactg agctttgtat ctgctctatg gccgcatctt ctgtcggaaa
     2161 ttccctttat tcttctgttg tttttatatc cataacctgt tccaagatct gtgctgcgtc
     2221 tttgatacat ccgtcacagg accgcagaac cttgcctgtc ccgatccctt tcagatcctt
     2281 gcagaccacg gaaccgttct tctctccaaa tttctgaacg atctctctgc tgacacgata
     2341 tgtcgcacct tttgtgaccg gttcttttgt attgcggctg ctctttaaaa gtcctgtcac
     2401 catacaggct cccgaaacgg ctccgcaggt acactgcata ccgcccatac cggctccgaa
     2461 tccttctcct gccgcaaaaa taagattttc atccactccc accagatctg cataggcaca
     2521 tgccactgcc tgacagcagt tgtaaccctt cccatgattc tccagcgctt tttctactct
     2581 gctgctcatt ttgattcctc cattgtaaaa ccacgtttct cacgccgaat aatattgcaa
     2641 ttttacacta ctttcgctca aattacaaga ggtatcagga ttttcacata gaaattctcc
     2701 cctcttgagc cgtattgaaa ctgaccgcca cggtttttga ctgtttttct tacattgtga
     2761 atcccatatc catgcattct gtcgtctttt ttggtcgtcc ggaagattcc ctttcttacc
     2821 tgtgcatgtg cttcgcagct gttctccacg atcagcataa acatcgaatg atgttttcgt
     2881 acttcaatct ttaccatgcg tttttcttct tccagctttt cacatgcctc tattgcattg
     2941 tccagaagat ttccaagcag aatgcacagt tctttatctg caagagcgat ctctccgatc
     3001 atatctgtct taatttcata taaaattttc tttttttcca tggcaagcag cttatattcc
     3061 aggatcatgt caaggatcaa attctgcgtc cactgcctgt gccggtcttt ttccaacggc
     3121 cgcagttttc cgatatattt atggagacgc tcatactctc ctttttctgc cagcttccac
     3181 atcaccagat tgtgattgtt catatcatga tataaacggc ggtttttttc gtattccatc
     3241 gccagctctt catactgatg ctctgccata tccacccgca tctgcaaaat ctcttctttt
     3301 tgtttcatat cgtggtagat cagataaatc atgcacagaa tccccagaaa tacaaccatt
     3361 gcccagatac tgctcttgaa cacgatctta ctgctgctcc cgagcagaac attctggaaa
     3421 tagaatacgg cagaagatcc gatcagaaac agcagcgaaa cgagcatcca gttgacttcg
     3481 atctcatggc ttttctgcca ttcacagatc ctcaatacca gaatacagac catgatcctg
     3541 ctgatattca atgccagtat gctgaccatc ccaacgtctg catactctgc tccgatgcag
     3601 aacagcagta caatccaggt gcgactcagt tcctccagta cctgaaaaca gccaaacacc
     3661 agaaatgccc gccagacgga annnnnnnnn nttactgccc acagaagcag tgccagaatg
     3721 ccgcttttga atataaaaat ctcagctgcc gaaacacctg ctttacccag gcatctgaaa
     3781 aagacattca tccccaccga agcacacaga tagatcaggc tttcttttcg ctctaccatt
     3841 tccagcttca gacctctggc agacacatca catatcagaa acacttccaa aaccacgatc
     3901 agatactctg caattgcaaa accggtcatc tccatcctct ttccatatat tcactcattt
     3961 tctgcatgat ctctctgtta tgtcctcttc ctaccttgat caaatcccca ttgtccatcg
     4021 taagcattcc cttttccagg cgttccacat gaagcagatt cacaatatat cccctctctg
     4081 ccatgacaaa ctgctctttc gggagttttc cggcaacctc ttttaaagat tccctgtttg
     4141 ccttttttcc ctgaagtgtc acaaaaatgg aattcttctg ttctttataa atataacaga
     4201 tctcacgcag tgctactttt tcgaacacgc ctgctctttt taccagatac tgtcctttga
     4261 catgtatgcc gatctcccgg cagatctgcc gcatcgtctg taccagtttc ttttccatat
     4321 tttctttgat aatataatga aatgcccgga cttcgtaccc ggaaagtgca tatttttcgt
     4381 atgcagtcag gaacacgatg tatgccctct cctgtatttt tctgatctct tttgcaagct
     4441 ccagtccatt cccgtccgga agttcaatat ccagaatata aaccgtgaac aattcctgcg
     4501 acagctcact ttgcagctga ctgaatgtag aaaaacactc aacttgaaaa ttttcctctt
     4561 tgaaattccg ttctatcact gccttcgtgc tttctctgac catatagtca tcatctacaa
     4621 tcgctattcg gtacataacc aattctcctg tatttttaag tacattctcc ttatagaata
     4681 tataggaatt atcagaaaaa atcaattact tttgtgcctc agtcgaaaac agggagctct
     4741 tcttcagagc tccctgtgca taagctaggc ggatacaact cacactcgtt tactgactca
     4801 cacttcaatc ccatattcta aaagcccacc tacctgagat gattatatgg ttttttgtct
     4861 attttgtacg cttttgtccg taaattgtct ttttttgtcc gtaaattgca aagatgccag
     4921 ggtcataagc ctgaaaccac gatgtgcttg cacaggagtg gtttacgact taatgacccg
     4981 ccccgacatg agcataaaag tggagcgtaa gcttcacgat atgcgaatgt cggacagaat
     5041 tgtgagagta cgaagtacgg aacaattcgt atggcatctt ttgtctataa tatcttgcaa
     5101 agatatgtta ttcctgatcc agaatttctt tcaaaatttt gttaacactc gccggatctg
     5161 cttttccttt catggctttc atggtctgtc ccaccagaaa tccgatcgct tttttcttgc
     5221 cgcctttgta atcggctacc gactgtggat tctcagaaac aatctgctcg atagtggcct
     5281 taagtgcacc ttcatcgttc accgttttca gtccatgttc ttcgatgtaa acttccggat
     5341 caacattctc tttgaaaatc tgctcaaaga cttctttcgc caccgaactg ttgaccgttc
     5401 cggcatctgc aagatctacc agttttgcca gattttccgg tgcaaaagaa agatcttccg
     5461 gatccatgtt gttttctttt aacagtcgca gggtctcacc catcagccag ttggatactt
     5521 ttttcggctt tttgcagatt gcagttgccg cctcaaacag atccgccata tgtttggagt
     5581 ttgtgatgat ctgtgcatca taatccggaa tatcaaattc ttttttataa cgttccagtt
     5641 tctcgctgcg aagttccggc tggcgtgcct tcacctctgc gatccattcg tcactgatca
     5701 caaccggtgt cagatccgga tccgggaaat aacggtaatc ctgggcatct tccttggaac
     5761 gcatggtaaa ggaactttct ttgttgtcat cccatcttct ggtctcctga atgacctttc
     5821 tgcccgcctc gatcagctcg atctgacgct ctctttcgcc ttcgatcgca tgtgcaatgg
     5881 ctttgaaaga gttcaggttt ttcatctcgg ttctggtgcc aaacttttct gctccgactt
     5941 cgcgcacgga taaattgaca tcggcacgca tcgagccttc ctgcagtttg cagtctgatg
     6001 ctccgagata ctggatgatc tgacgaagtt tttccagata ggcaatgact tcttctgcac
     6061 ttcgcatatc cggctcggac acgatctcga tcagcggcac accgcttcgg ttgtagtcta
     6121 ccagggagca gtcttcccac tcatcgtgca ccagttttcc ggcatcctct tccatatgga
     6181 tttcgtgaat cccgatgatc tttttgccgc cggctgtctc gatctccaca ccgccgtcat
     6241 gacagattgg cagatacagc tgtgagatct gatagttctg cgggttatcc ggatagaaat
     6301 aatttttacg gtcgaactta cagtactgat ggatctggca gtttgttgca agtcccaccg
     6361 caagggcata ctcaaccacc tgtttgttca gtaccggaag ggaacccggc ataccggtgc
     6421 agaccgggca ggtatgggta tttggtgctc ctccgaaagc agtggagcag gcacagaata
     6481 tttttgtttt ggtcgcaagc tctacatgga cttccagacc gatcacggtt tcatactgtt
     6541 tactcatctt attttgcctc cccttctgct gtttttgcaa gctttggtgc ttcgtatttc
     6601 ctcgtctgtt cataagcata cgccgcacgg atgatattct tttctttgaa gcagtccccg
     6661 atcagctgca gaccgatcgg cagccctttt gcatctcttc cgcacggaac gctcatgccc
     6721 ggaagtcctg ccagattaac agaaatcgta taaatatcac cgagatacat tttcagcgga
     6781 tcacttaaag aatctccgat ctttggtgcg gtattcggag ccgccggtcc taagatcaca
     6841 tcatattttt caaatgcttt gtcaaatgct tttttgatca aagcttttgt gcgaagtgcc
     6901 ttcagataat atgcatcgta atatccggaa ctcagtacaa aggagccaag catgatacga
     6961 cgttttactt ccgctccgaa gccctcagaa cgtgtctttt tgtacatgtt gtgcagtcct
     7021 tcgtaatctt ttgcacgata gccatatttc acgccgtcaa atnnnnnnnn nngatgacat
     7081 agtaagccgg gattgcatat tctacaaggc ttaaatcaaa ctcttccaca atagctccct
     7141 tttcttccag cacttttgcc gcagccagaa ttgcctcttt gacttccgga tccagtcctt
     7201 ctccgaaata atctctcgga atgccgatcc gcatgccctt cacatcatcc acaagagcag
     7261 aagtgaaatc ataatcttcc cgttttacgg aagtgctgtc ctttttatcg taagatgcga
     7321 tcgcctccag gattgtcgca cagtctgtca catcttttgc caccggtccg atctgatcta
     7381 aggaagaacc ataagcgatc aatccgtaac gggaaacggt tccgtaagtc ggttttaatc
     7441 ctgtcacacc gcagaaagaa ctcggctgac ggatcgatcc tccggtatcg cttccaagtg
     7501 caaagagaca ttcacctgct gccacagctg cacaggaacc gccggaagag ccgcccggta
     7561 cgtgttccag atcccatgga tttctggtca caccataggc tgatgtctca gtcgtacttc
     7621 ccatcgcaaa ttcatccatg ttggtctttc cgatgattac cgctccggct ttttccagat
     7681 ttttgactgc ctccgctgtg taagtcggtt tgaagtttga aaggattttc gaactgcagg
     7741 tagttaaaac tccctctgta cacatattgt ctttgacagc taccggaaca cctgccagcg
     7801 gaccgtcaag ctctcctgca tcgatctttg cctggatctc ttccgctttt ttcaggactt
     7861 tttctttgtc tttcagtgtc acataggcgt gaacgtctcc ttccactgcc tcaatctgtt
     7921 cgagtacggc acttacagct tctgcaactg taatctcttt cgctttgatc tttttgccaa
     7981 gcgtcactgc tgtcaattct gttatcttca ctgtgtgcct cctattctac cgttcgcggt
     8041 actttatatg ttccgtcttt ttctgccggt gcatttttca gcatattgtc tctgtcatcg
     8101 ccgtttgtga ccacatcctc acgaaataca ttgtacaccg ggaaaatatg ggacatcggc
     8161 tctacacctg ctgtatccag ttcatttaat ttatcaatat aatccagcat gcgttccata
     8221 tctttcctgg ctgctttctg ctcttctttt gaaagttcca gctttgccag aatgcccaca
     8281 tactccattg tctcatctga gatcacattt gccatgttct ttctcctctc tttgccgcca
     8341 tataaaatat cttttctttg caattcttat tctattatct gtcttatttt tgtttttcta
     8401 tggctccagt ctgctcaagt ctctcgggaa gagggttgtc tctcttacgt tgtcctcgct
     8461 gatcagctgc atggtcagtc gctccagccc gataccaagt cctccgtgag gcggcatgcc
     8521 atgacggaac gtatcaagat actgctccag tccttccgtt gtcattttct tcgcttcgat
     8581 cttgtcaagc agctgctgat aactgtggat acgctgtcct ccggttgtga tctccagtcc
     8641 gcgaaacaga aggtcaaaac ttaaggtaaa tcttgcatcc tgcggatcat ccatcgcata
     8701 gaacggacgc tttttgctcg gataatgtgt gataaacaca aaatctgcac cataatcttc
     8761 tttgaacagc tccccgatca gatgctcttc ttccggctcc aggtcgtatg gattgcggat
     8821 cgtacggttg tatttctctg caaccatctc ttttgctttg tcaaaacgga ccgccggaat
     8881 tttcgataca tccggaagtg tgatgccaag catatccagc tctttctgat aatcctttgc
     8941 cagaagtttc atcgtgtact gtaaaaatcc ggtctccatc gccatgatat cttcaaatcc
     9001 gtcgatgtat cccatctcaa aatcaagaga tgtatactcg ttcaggtgac gttttgtgtt
     9061 gtgtttctct gcacggaaca ccggtcctgt ctcaaatacc cggtcaaaca caccaaccat
     9121 catctgttta taaagctgcg ggctctgctg caggatcgcc ggtctgtgga aatactccag
     9181 tttgaagagg tttgcaccgc cctctgcact tttggcccca agcttcggtg tgtggatctc
     9241 cgtaaatccc tgctcataga ggaaatcgcg gaaactgcgg accagacctt cctggatctt
     9301 gaatttcgca cgttctctga catttcgaag agagatcgga cgcatgttca gtttcgcttc
     9361 cagtgaagta ttcagcttcc atttggaaat cggcagcggc agcggttcat ccggctcgct
     9421 gagcactttg atcnnnnnnn nnntgtgcag cacgttcttc tttgcggatc gtaccgagta
     9481 cttccaccgc agatgcttct ttgagttctt ttaagtcaaa ggctgtcaca ccttcttcaa
     9541 acacgcactg caccagaccg tcacgcttac gcaggatcac aaatgccaca tttcccatat
     9601 cacggatcgt atgcacggct ccgtttactt ttacctctgt gccttctgcc tgcaccagta
     9661 actcaccaat ctccaatgtt tttgcttttt ttacgccttc taaatactcc atcatagtct
     9721 ttcgtctcct ttcaactttt cgctgtccgg gaaaagaatg gaataaaaaa gtcccgaaac
     9781 agcactgaac tgtttcggga cggataaact ttatccgcgg taccacccgc cttgcagata
     9841 aatcatctgc ctctcttgac gtttaacgca cgctcacgta ccgccctacc tgtatctcct
     9901 tcgaacggtc tgctccgaag tgttcccatc ttgccgcttc cgccaaaaag tgctctcagt
     9961 cggtgacact ttattcctgt cggctttcac gatcatgagt cttcatctca gcatttgcat
    10021 gatgccaggg gcaaaagcct gaaaccacga tatgctcgca caagagtggt tttatggcat
    10081 ctcttaatcg ttttatcatt tttatgattc aaannnnnnn nnngacggaa ataaccttct
    10141 ccgcttggtc cgaatccgga acccggtgtt ccgaccacgt ttgctttttc cagcagataa
    10201 tcaaagaatt cccaggaatt catctgctcc ggcgtcttca gccatacata cggggagttc
    10261 acgccgcccg caacggtata gccggcttct ttgagtccgt tgaagatata agatgcattc
    10321 ttgtgataat atgcgatctg ttctgcgatc tgagcctttc cagcctcgga atataccgct
    10381 tctccggctc tctggatgat gtaaggtgca ccgttgtatt ttgtgccgtg tcttcttgcc
    10441 cagagtgcat gcagctgtac atcgccgcat ttgagctctt tcgggataac cgtgtatgca
    10501 agacgcagtc cggtaaatcc ggctgtcttg gaaaagctgc aaagctcaat ggcacaggtt
    10561 tttgcacctt cacactgata aatagacact gggatatctt tttctgtgat ataagctttg
    10621 taagcagcat cgtagatgat gactgcacct gctttattgg cataatctac ccatacctga
    10681 agctgttctt ttgtgatcgc ctcgccggtc gggttgttcg ggaagcacag atagataata
    10741 tccggagtct ctttcggcag ttccgggcag aaattatttt ctgcggtaca aggcatgtag
    10801 atcacatcac tccaggtctc acttttcgga tcgtaagtgc cggttctgcc tgccataacg
    10861 ttggaatcca cataaaccgg gtaaaccgga tcacataccg caattttatt gtccagagag
    10921 aaaatctcct gaatatttgc agaatcgctc tttgcaccgt cggatacaaa aatctcatct
    10981 gctgcgatgt cacatccgta agcttcataa tcattctttg cgatcgtctc acgcagaaat
    11041 gcatagccaa gatccggtgc ataacctttg aatccctctg ttgtggtctg ctcatcaact
    11101 gctttgtgca gtgcttcgat cacggccggt gcgatcggct gtgtcacatc accgataccc
    11161 agacggatga tctctgcctg tggattagcc gctgtaaatg cattgacttt tttcgcgatt
    11221 gtagaaaaaa gatagcttcc cggtaacttc agataattat catttacttt aaacatatgt
    11281 gcctcctgtg attctcattt ctgccggcag ttccggcatt tttataaagt ggatcttcat
    11341 cgcccgcttt tgcttttact catggcagtc tttttcatct gccttgtatc gttacaattt
    11401 aatttcgccg tcgaagacga cttctgcagg gcccgtcata aagacagtat tcttctgctg
    11461 atcccagaaa atctccagat ctccgccaag cagttttatg gtcaccggct gatcttttac
    11521 cacataccca ttcagaatgg atgccactgc caccgcacat gcaccggttc cgcaggcaag
    11581 tgtctcaccg gctccgcgtt cccatacacg catctcaaca gtatgatcgt ccagtactct
    11641 ggaaaattct gtgttgacac gatccggaaa acacggatgc gtctcaaaaa gcggtccgat
    11701 tttttcaata tccagacctt ttacatcgtc tatataaaca actgcatgcg gatttcccat
    11761 agaaacaccg gtcatgcgat attccctgcc gcctgccgtg atcgcttcat tgatgacctg
    11821 cggattctcc gatacgatcg ggatcaggcc tgcatttaag atcggtgccc ccatatccac
    11881 acggaccgtc ttcacttttc cgttttccac ctgcagtttc acgtatttga tgccgctctt
    11941 tgtcgcaatg ctgatttcat ctttatctgt aagtccatag tcataaacat actttgccac
    12001 gcagcggatc ccatttccgc acattgctcc ctgagaaccg tcggaattat acatatccat
    12061 ctcaaagtct gcttccagcg aaggtttgat caggatcagt ccgtccgagc cgatcccaaa
    12121 gtggcggtcg ctgacctttc tggcaacttc tcccggattt tcaaccgtct cttccagaca
    12181 gttgacatag acatagtcat tnnnnnnnnn ncaagatctc cgatacgcag cggcagcggc
    12241 agagatacaa tatccctgtg gtcgcaggac tccaaaatat gcatagatgc taccaaaagc
    12301 atctggaacg aagatgccag actttccctc aaatccgcat acatcatatc gttaaaacgg
    12361 tatccgcaga tcacaatgcc gtcttccaga ttgtctataa tctgcaatac ctgtgcaccg
    12421 gtacagcata caccgatcac gctgtatcca tttctcgcca gaaggttacg gatatttttc
    12481 gcatcttcca gctttggaaa tacaactatt atgtttacca ctataaccct cctcctcttg
    12541 tacaatctta aaaatcaaga tccatctcgg gaggaaaatt tatatgcggt aaagatactg
    12601 atccagttcc catccggaaa cctggcggga atattctgcc cattctttct ttttcgcctt
    12661 gatataacgc cggctggtgt gtgttccaag cacctctttt accagctcgt ccttttccat
    12721 ttcatagact gcttccagaa gattgttcgg caggacatca ataccctctt cctggatctc
    12781 ttcttctgtc atattgaaaa tatttctatc aacagctgcc ggaggcatga tctgcttctg
    12841 gatgccgtcc agtcctgctg ccaggcaaac tgccagcacc aggtatggat ttgccgacgg
    12901 atccggacaa cgcagttcta ttctcgtgcc ctccccacgt tctgccggaa tgcggatcag
    12961 cgggctgcgg tttgtgcctg accaggcaat gtaaaccggt gcctcaaatc ccggaaccaa
    13021 acgtttgtaa gaattgacca gcggattggt gatcgcacac attcccttga catgccgcat
    13081 cagaccgcca ataaaatagt atgcttcctg actcaggcca tttggatcac agtcatccgc
    13141 aaaaatattt tttccatcct ttgacaggga catattgatg tgcatgccgg agccattgat
    13201 accggtcttt ggctttggca taaagcttgc atgcagtcca tggcgtttgg cgatcgtctt
    13261 gactgccagc ttgaatgtca tgatattgtc tgccgtcgtc agtgcctcgt cataacgaaa
    13321 atcgatctcg tgctgtgccg gtgccatctc gtgatgagat gcctcgatct caaatcccat
    13381 atcttccagc gtcagaacca tatcacgcct tgcattctca ccaagatcca tcggacctaa
    13441 gtcaaaatag cctgcacgtt catgtgtgat cgtcgtcggc tgtccattgt catccgtatg
    13501 atacaagaaa aactcacatt ccggtccgac atcaaactga taaccaagct ctgccgcctg
    13561 ctttaccact tttttcagaa tatatctcgg atccccctca aacggctccc gattcggacg
    13621 gtacacatca cagatcagtc gtgccacctt tccctgctgc ggccgccacg gaaaaacatt
    13681 taacgtatcc agatccggat aaagatacat gtctgactcc tcgatccgca caaaaccttc
    13741 aatcgaagat ccgtcaaaca tacagttgtt atccagtgcc ttttccagct ggctggaagt
    13801 gatcgccaca ttcttcnnnn nnnnnncttc atcgttccga aaatatccgt aaactgaagg
    13861 cgtataaatt ctacatcttc ctcctgcaca agacgcagga catcctgttt tgtatacttc
    13921 atatattctc cttttatgat tcccattaga aaaaacctga ttccctgtaa atagaaaaga
    13981 agggtgcact gaataaatca gaacaccctt gttccagtca aattagtttg cattttcagc
    14041 atccgctcat gacgaagtca caagtgctct gctttttcat aaaagccttt cttttatatg
    14101 atagcagtgc cgcctttatt tgtcaaccgc acggttttca ttttttatca ttccagcccc
    14161 agcggacttc tcacatactg gaatccggct gaccatgctt ctcctgtcat aactccgttc
    14221 tggcttgagg agcagtgtac gaccagcagg cttccgttgt cattgactcc gactacgatg
    14281 ccgacgtggt tatcgtctcc tgcacttgcc ggatgataaa agacaagatc accaggtctg
    14341 gcactctggc tgtccaccat ctcgcttgca ttccactgtg ttgtggtatg attgccgata
    14401 ccggcatcct tgccgccaag tccgttatag taagaccagg caacaaatcc actgcagtcc
    14461 agaccatagg aacgcaccgt accactgctc ttgctgccct ctgcggacac ggtcatcgga
    14521 cttccccagc tgtcatccca gccgattgca taggacttgc cgccccagaa atagccgact
    14581 tttccgatca ggctgcacgc tgctgccacg atagaaacac gttcttcact gacatcgctg
    14641 cctgcctcag aacgcacaaa tcctttggat gccgtaacga ttgcacagag ctgtctacat
    14701 tttgtggacg tgtatttctc cagtatagct gtctgctctt catctgcacc gtaaagtgct
    14761 atgtaatcct gaaccgacag tgcacggtat tcttttgcat ctgcttctgc cacttttttt
    14821 gtttctgttg attcttccgc atcagcttcc ttttctgttt ctgttgattc ttccgcttct
    14881 gccgcctttt ctgtttctgt tgattcttct acttctgccg ccttttctgt ttcggctgat
    14941 ctgccttcgg tttcagagac ttcctctttc tgaattttct ccagtttgcg taaaattgca
    15001 gaattgcttc tgacattcat caggaaaaaa atctgctcca gatcatccct gcaggacttg
    15061 tccatcttta tgccggcgtc ctgtccgtac ttca
//
LOCUS       DAWDWH010000024        15071 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_2250_length_15071_cov_10.962706, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000024 DAWDWH010000000
VERSION     DAWDWH010000024.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 15071)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15071)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15071
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2250_length_15071_cov_10.962706"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6812..6821
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10826..10835
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11555..11564
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14125..14134
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14649..14658
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4717 a         2948 c         3926 g         3430 t
ORIGIN      
        1 aacttaagaa aaatgagtat tttatcgcag atctgatcgg acttaaggtc tatactgagg
       61 atgatgcatt tcttggcaca ctggatgatg tgatccagac cggagcaaac gatgtctatc
      121 aggtagtgac ggaagaagaa aaagagatcc tgatcccggc gatccgtcag tgtattctgg
      181 aagtagatgt ggaagaaggc cgcatgaaag tgcatcttct ggaagggctg gtgtagcatg
      241 cattttcacg tactgacact gtttccggaa atgatcgagc agggaatgaa taccagtatc
      301 atgggcaggg cgatcgaacg gggatatatt tctctgaaag caacggacat ccgcgactat
      361 gctgcgaata agcataataa ggtggatgat acaccgtacg gaggcggagc cggcatggtc
      421 atgcaggcag caccggtcta tggagcctat gaggcagtaa aagaacaaat cggttaccgg
      481 ccgagagtga tctatctgac accgcaggga agtacttttt cacagcctat ggcggaagaa
      541 tttgcaaaag agcaggatct ggtctttctg tgcggacatt atgaagggat cgatgagcgt
      601 gtcctggaag agatcgtgac agactacgtc tccatcgggg actatgtcct gaccggaggg
      661 gaacttccgg caatggtgat gatggatgcg atctccagac tggttccggg tgtgcttcac
      721 aatgaggcat ctgccgaatt cgaatctttc cagggaaatc tcctggagca tccgcattac
      781 agcagaccgg aagtctggca tgaaaagcgg gttccggaag tgctgttatc cggacatcat
      841 gccaacattg aaaaatggag aagagaacag tccgtcatcc ggacggcaga aagaagacca
      901 gatctgctgg aaaaagcgga tctgtcagca aaagaaaaag ctctggcaga tcgctgtctg
      961 gctgaaaaaa agcttgcttt tacgcaagag gtatgataaa attattcacg ttgtgagaag
     1021 agatggtcct ctgctacctg ggcggtatta agaacatctg aaaatataag gaggaacaac
     1081 aagatgaacg aaatcattaa gaacattgaa gcagctgaac tgaaagcgga agtacctgta
     1141 tttaacgtag gtgatactgt aaaagtttac gcaaagatca aagagggtaa ccgcgaacgt
     1201 atccagatct tcgaaggaac tgttctgaag aaacagggtg gaagcaacag agcaactttc
     1261 acagtaagaa agaactccaa cggaatcggc gttgaaaaga catggccgct gcactccccg
     1321 aacgtagaaa agatcgaggt tatcagaaga ggtaaagtaa gaagagcgaa actgaattac
     1381 ctgagagacc gtgttggtaa aaaagcaaaa gtaaaagagt tggttaaata ataggacgca
     1441 gcagcgtacc gaatgattcc gcaacaagca ctgcttgttc gggtcagcca gacagaccag
     1501 ggacaagtac ttaggtaatt gtccctgttt ctgtataacc gactgtgaat ttcaaaaggc
     1561 aaaaggggag gatacatgag ccgcacagga agaaatcgca gagaaaggga tcgaagaaac
     1621 aaagatcgga tttcacagga acaggcaata caggaaaaac acagcagaag ggaaatgagg
     1681 aaacagaaaa aagaacggga aaaggtcaga aagaatccga tcaaagccgg tcttggatgg
     1741 ctgtttcaga tcattgtggt gatcctgttt gcatatattg ctgttttctt tttcggtcag
     1801 tccagaacga acatcggaca ggcgatgaac agtaccttgt ccggcggaga tgttgttctg
     1861 ttaaatgagc tgtcctatcg cttttcagga ccatccaggg aggatgtaat ctcgtttaaa
     1921 ctgaatggaa gcgaggacac gcattcttat ataagacgtg tgatcggact gccgggtgag
     1981 acgatccaga tcatcgatgg tatgatctat attaatggaa gtgtctacct ggagacgcag
     2041 ggatttccac agatggaaaa tgccggaatg gcagcacagg agatcacact tggtgaagat
     2101 gaatattttg tgcttggtga taaccgcaat cagagtgaag acagccgttt tgcagatatg
     2161 ggaaatgtaa agctggaaga tattgaaggg aaagtctggg ctgttttatc tccttcggat
     2221 caccggggac ttgttaagta gaaaggtaaa aaatatgaat tatcagtggt atcctggtca
     2281 tatgaccaag gcaaggcgta tgatgcagga agacatcaaa ctgatcgatc ttgtcatcga
     2341 actgctggat gcacgtgtac cgttttccag ccgcaatccg gatatcgata agatgggcag
     2401 ggacaaagcc cgtctgattc ttctgaacaa atcggatctt gcggatgaaa agagcaatga
     2461 agcgtgggtt gcttttttcg aagcgaaagg gtattgcgtc agaaagatca attccagaaa
     2521 tggagcagga cttaaatcca tcaatgcagc cgttctggaa gcatgccatg aaaagatcga
     2581 gcgagacaaa cgcaaaggaa tcaagaaccg tccggtgcgg gcaatggttg tcgggattcc
     2641 gaatgtcgga aaatctacgt ttatcaatgc ctatgccgga aaagcatgtg caaagaccgg
     2701 aaataagccg ggtgttacga agggaaaaca gtggatccgc ttaaataagg gtctggaact
     2761 gctggataca ccgggtattc tctggccgaa atttgaagac cagagtgtcg gtatgcgact
     2821 ggcaatgatc ggttccatca aagacgagat cctcaatacg gatgagcttg cactcaatct
     2881 gatccgtttc ctgatggaac attatccggg gagattaacg gaacgttatg gctgcgaaga
     2941 aaccgctcag gaagcactca cagtttacga ggagatcgca agacggcgtg gctgcatcaa
     3001 aaaaggtcag gaactggatt ataccaagac aggtatgctt ctgctggatg atttccgaag
     3061 cggaaaactt ggcaggatca cgctggaact tccgaaggga gagacggaag agcagtagac
     3121 agtattttgt gggaggagag tatgaaaaag ggacttatcg aggtgctgtt ttatatcgta
     3181 ctggtgtttg gtgtcagctt tttgctgaca acttatgtcg gtcagcgtac gcgggtaaat
     3241 ggagagtcga tgtatccgac gctgcatgac gatgacaatc tgatcgttga taaggtcagt
     3301 tatcgtttct ctgatcccag acgttatgat atcattgttt ttccgtatcg ctataaggat
     3361 atgtatttta ttaaacggat cattggtctg ccgggtgaga cggtgcagat cttggacggg
     3421 tatgtgtaca tagacggtaa aaaactggac gaacatttct gtgatgaaaa aatccagaac
     3481 gcagcacttg catccgaccc gatcacactt ggagacgatg aatattttgt gctcggagac
     3541 aatcgcaatg ccagtgaaga cagtcgtttt ccggatgtcg ggaatgtcag acgcaaagag
     3601 atcatcgggc gtgcctgggt acgcatctgg ccatttgaac gggcgggatc tttgtagaaa
     3661 aggagttttt gatgaaagca aaagagatta ttaactgggt tgtatatttt gtatgcctgt
     3721 tagcagttgt attcgtgatc aatacatttg ttgtacagag gaccatggtc agcggagatt
     3781 ctatgtatcc gtatctgcac aacaaggatc agctgatgat ggataaatta tcttatcgtt
     3841 tccatgatcc ggagaggttt gatattgtag tctttccggt tgtgcgtgac ggaaaagaag
     3901 agtactatat caaacgtgtg atcggtctgc cgggagaaac cgtacagatc ctagatggct
     3961 atgtttacat taacggtgaa aagctgaatg aaaattacgg tgcagaagtg atgaatgatg
     4021 ccggacgtgc ggcagagccg atcacgcttg gcgatgatga gtacttcgtg ctgggagata
     4081 accgcaaccg aagcgatgac agccgctatg agaatgtcga aaatctcaaa agagaaaaga
     4141 ttgtcggtcg tgcctgggtg cgtatctggc cgcttgatcg tatctgcgta gtcagacacc
     4201 agtagacgaa aaaagagaaa tgcgttagaa taagaagtgc cactttgtgt ggcacttttt
     4261 taaatacgaa cgaaatgatc agtcggactt tttagacagg atatgcgttc atgagcaagt
     4321 agcgaagcgg attgtgaatg tccgctttac cgcagagaag cattcccccg ctaaaatatg
     4381 attaccgact gtgggttgga ataaaggaga aaatatatga gcacagacag aaaaagcatc
     4441 gcacagatca aaaaagaatt tgcagaggca aaagaaggtt ccctggaaga actgtttgca
     4501 cagtatgcta tggatgagag aagcggcgtg aaggcagttt taaacagtta ccgaaaaaaa
     4561 caggaaaagc tggcaaaaga gcgggttcgc ctggaagaaa tgagaagtta cgaacatcag
     4621 tacgaaagat atggctatgt gtgtggaatt gatgaggcag gacgcggtcc gctggcagga
     4681 ccggttgtcg ccggtgccgt gatcttgcca gaagactgtg agatcctgtg gttgaatgat
     4741 tccaaacagc tttcagaaaa gaagagggaa gctctgtacg atgagatcat ggaaaaagca
     4801 ctggcagtgt ccgtcggcat ggcatcaccg gcaaggatcg atgaaatcaa tatcctgcag
     4861 gcaacttatg aggcgatgcg tgaggcaatc gccggactga agatccggcc gcaggttctc
     4921 ttaaatgatg ctgtgaagat cccacaggtg gagatttcgc aggtaccgat cattaaagga
     4981 gatgcaaaaa gcctgtcgat cgcagcggcg agtgtgatcg caaaagtgac cagggaccgc
     5041 ctgatgaaag attatgacaa actgatgcca gaatatggat ttgcgaaaca caaaggctat
     5101 ggaagtgccg cacatatgga ggcaatacgc aaattcggat catccccgat ccaccgacag
     5161 agctttatca aaaacatcat atgagtcggg gggacaaaca ggtcaaaact gtttgaaagt
     5221 gcgaaacatg aaaaatcgca tataactcat aatacaaaag aaaatggggg tatcaatgac
     5281 aagaggggga aacacacgca aaactggggc aagatatgaa ttactggcag ccgtccatct
     5341 ggaaaaatgt ggttatcata tcatagagaa aaattatcgc tgccggatcg gggagatcga
     5401 tctggtcgcc tgcgacgggg cgtatctggt atttgtggag gtcaaatacc gcaaaaacaa
     5461 caagaagggc ggtgcggcag cggccgtatc cacagcaaag cagaaaacga tcagccgcgt
     5521 ggcagattat tatatgaaag ttcacgggat ctgtgcagac cagagtgtcc ggtttgatgt
     5581 cgtggcattt gacggagaag agatggaact gatccgggat gcgtttgcct atgccggaaa
     5641 tgtccggttt tgatcggtat ttagcagagc aatttgcagg catctattgg ggtcaaaaga
     5701 acttttgaca ctggcatttc aggagtttac aggaggaaga tagatgggta cttttgaaaa
     5761 aagagaaaaa aacggtgtga catggctttc cgctacgccg tttgaaaaga tggaagggat
     5821 cgtgcaggga ttttcaacgc ggcttggcgg agtgagcaca gagcatctgt cgagcatgaa
     5881 cctgagtttt tcacgtggcg atcaggaaga aaatgtacgt gaaaatttca gaagaattgc
     5941 agatgcgatc ggtttcacac cggaagatct ggtcttttca gatcagacgc atacgaccaa
     6001 catccgtgtg gtgacagaag cagaccgcgg caaaggattt acaaaaccgc tggattatac
     6061 ggatgtggac ggactgatca cagacgttcc gggactggtg ctggcaacgt tttatgcaga
     6121 ctgtgtgccg ctgtattttg tagatcccat cagaaaagca gtcgggcttt cacattccgg
     6181 ctggcgtgga acggttcata agatcggcaa gataacggtt caggcgatgg cggatcaata
     6241 tggaagcaga ccggaagata tcgtagcaat aatcggacca tcgatctgtc aggactgtta
     6301 tgaagtcagc gaggatgtca ttctggaatt tcagaaatat tatcgtgaag actgtcagag
     6361 cgaattgtat taccggaaag aaaacggaaa gtatcagctg aatttatgga gggcaaatga
     6421 gattgtgatg gaggaggccg gaatcttgcc ggaaaacatt catacgaccc agttgtgtac
     6481 ctgctgcaat ccggaacttt tgtattcaca ccgtgcctca aaaggcaaac gtggcaatct
     6541 ggcagcattt cttggactga agaggagaga atgattttat gaaacagatg atcaacacaa
     6601 cggattatgc gtttgatgtg gaacgctatc gggatgaggc agatgccatt tcgtttgcag
     6661 gaaatcttgg tatggatgga tttgaactgt tgccgtgtga gggaggaaga gatgatttct
     6721 ttacagaacg atccgtgatt ggcgtacatc tgcgatattt tgattcctgg ctggatttct
     6781 ggaatggaaa actggagatg ttgagcaaag annnnnnnnn nacggatcct ttgaaaaagt
     6841 gaaagagatt ttcggagggc tcgatcgcag ctgtatcctg aaacgttttc gggaagacct
     6901 ggagcgtgcc agaaagctga aagcccgcta tgtggtcttt catgtaagcg atgtcagtgc
     6961 cagggagctg tttacctatc agtgccggca cacagacgaa gaagtgatcg atgcggcggc
     7021 agaaatgatc aatcttttgc tggatggaga agattacacg tttgatttcc tcatggaaaa
     7081 tctctggtgg ccgggtctta ccatgacaag gccggagatg acgaagcgtc tgctctcgca
     7141 ggtccattat caaaagaaag gcattatgct ggataccggg catctgatgc acatgaatct
     7201 ggaactgaaa acgcaggatg aggcggtcga ttacatcctg gaaaaagttg cagagcatgg
     7261 aaatctggct tcttatatca aaggcatgca tttaaatcag agcatcacgg gtgcgtatgt
     7321 gaaaacgctg atcaccaaaa aggatgccat gccatcggat tacgaagcat gttccaaagc
     7381 ctgctatgaa cacgtcttcc agatcgacca gcatctgcct tttaccacac cgaaagttca
     7441 gaaactggta gaaaccataa aaccggaata tctgacacat gagctgatgt cctacggaag
     7501 aagcgagcat gagatcaagc tggtgatgca gcgtgcagct ttgaagggga aaaatctata
     7561 ataaccattc aggctttgcc cgtgacagct ttttaaaacg agaaaaatta tcaaatacaa
     7621 atcagaaaaa agcgagacaa ctgtgctata atgaccataa gttagttgat tctaacatca
     7681 aatataaagt gactaccgcc tttgcagaca gtgagcgaca agttacagac tattttttgg
     7741 gagaggaaaa atatgttttt agagatcaaa gacttgaaaa aatcatttgg cacagcagaa
     7801 aacaggacgg aagtgctgaa aggaattgac ttttctgttg aaaaaggaca gatctgtgtg
     7861 ctgcttggac catccggttc cggaaaatct acgcttctga atattatcgg cgggattgac
     7921 caggcggacg aagggtatgt ctccatcaat ggtgagaaaa cggcggatat gaaagaaaaa
     7981 agactgacgc aataccgccg ccgacatctg ggatatgtat ttcagatgta caatctgata
     8041 ccaaatctca gtgtcagaga aaatattgaa gtgggggctt atttaagcga cagaccgctg
     8101 gaactggatg agcttctgaa aacacttggg ctgtatgaac atcgcaacaa gctgccgaac
     8161 cagctttccg gcggacagca gcagaggacg gcaatcgggc gtgcaatcat caaaaatccg
     8221 gacattcttc tgtgtgatga gccgacgggt gcactggatt acaagacttc caaagaaatc
     8281 ctctgcctga tcgagcagat caaccagaaa tacggcaaca cggtgatcat ggtaacgcac
     8341 aatgatgcca tcaaaaatat ggcagaccgt gtcgtaaagc tcagggacgg acagatccgg
     8401 aaaaattatg tcaatgaaac caagatcgca gcagctgatc tggaatggta aggcggtgag
     8461 aatatgaaaa atccattgct caaacgtctt ccaagagaac tgatcggtga cattggaaaa
     8521 taccttgcag tttttctttt tatgacagcg acgatcggat ttgtctccgg tttcctggtg
     8581 gcagatgaca gtatgctggc agcttatcag gaaagctttg agaaatatca catcgaagat
     8641 ggaaacttta cattggagaa aaaggcaacg gacagtcaga tcaaaagact ggaaaaagaa
     8701 ggcgttcaga tttatgaaaa ttactatgtc gaagaagatg cagatactga tctcgacggg
     8761 gaaaaggata gtacagtccg cgtttacaaa aaccgcagcc agatcgatct cgtatgcctg
     8821 atgaaaggag agcttcccaa gaaggaagat gagattgcca tcgaccgtat gtatgcagaa
     8881 aataacaaga tacagatcag tgacatcata aaagtcggaa aagaagaaaa aacggtgaca
     8941 ggctatgtgg cactgtctga ttacagtgca ctgttttcca ataattcgga tatgatgttt
     9001 gatgcagtta agttcggtgt tgccattgtt acagatgaag cctttgacaa tctggaagaa
     9061 acgcacctta aatatcgtta ttcctggaca tatgatgatc cgccacaggg agaaaaagca
     9121 gagaaagaac gttccgatga ctttcttgag atactggcag attacacatc ggtgacaggc
     9181 tatatcccac gctatgcaaa tcaggcgatc catttcaccg gagacgatat gggcagtgac
     9241 cgttcgatga tgatcgtgct tttgtatatc ctgattgcaa tcatggcctt tgtttttgcg
     9301 gtgacaacaa acaatacgat tgtcaaagag gctgcggtga tcggaaccct gcgggcttcc
     9361 ggttatacaa gaaaagaact tctggtacat tacatgacac tgccgcttct ggtcacggtt
     9421 atagcagcgg tgatcggaaa tgttctgggt tataccgtct ttaaaaatat ctgtgccggt
     9481 atgtattatg gaagttacag tcttccgacc tacgaaacga gatggaatat ggatgcattc
     9541 gtcctgacga ccattgtgcc gtttgtgatc atgctgctgg tcaatctgat cctgatctcg
     9601 aaaagattaa aactcactcc gctgaagttt ctaagaagag acttaagcac atcaaaaaaa
     9661 caaaaagcag tgaaactgcc ggatattcgc ttttttgacc ggttccgtct gcgtatcatc
     9721 ctgcagaaca aatccagtta catcacgttg tttgtgggaa tcgtatttgc caatattctg
     9781 ctgctgttcg gcatgatgat ggcaccgctt ctggatcatt accaggaaca gaccgttgac
     9841 aatatgatcg cagattatca gtacattctg aacgtaccgg atgagattga tgaggatgac
     9901 acttacaccc tgtatggggc actgagccgc ttcctgactc cgtcactgga aacagaaaac
     9961 aaaaaggcag aaaaattttg catggaatca ctggaaaccg ttccgggcaa acgggacagt
    10021 gagagcgtca ccgtctacgg aacacagcag ggaagtcatt atatgaaggc ggattttccg
    10081 gatgagggtg tgtatatttc agttggatat gcggaaaaat acaatataaa aacaggagat
    10141 accatttggc tgaaagaaac ttacgaggat aaaacatact cttttgcggt aaaaggaatt
    10201 tatgtctatc cgtccggact ttccgtattt atgtcaatgc cgcagttctg ccgtgtgttt
    10261 gagaaatccg acacgtattt taatggctat ttctcaaacg aaaagatcac cgatatcgaa
    10321 gaagcgtaca ttgtttccac gatcacacag gacgacctga ccaagatttc cagacagctg
    10381 gatgtctcta tgggaaatat gtttcagctg atcaatgtat ttgcggttgt tctgtttgcc
    10441 cttctgatct atcttctgac aaaactgatc attgaaaaga atgaacattc tatttccatg
    10501 gttaagattc ttggctatga aaatgatgag atcgtccgcc tgtatctggt ttccacaagc
    10561 tgggtggttg ccgtttccgt attgctgagt ctgtggatcg ccacctggac gatcaaggtg
    10621 atctatgtct atatgatggc agagttttca ggctggcttt ctctgtacat tgagcctgca
    10681 atatactgga agatgtttct gatgggcatg gcagtttatg cattggttgc tttgctacag
    10741 ttttttagaa tcagcaaaat accgatggaa acggcactga aaaatatcga atagagaaaa
    10801 gtaaataaaa tctgctaaaa aatacnnnnn nnnnnatgtt tcagcagatg ggaaaagaat
    10861 gcacgtcatg cagacatggc gtgcattttt tgatgaaact tcttctgatt cagcaaatgt
    10921 taaaaaagag agtaacaata agagaggaga aaagcaaaaa gtatgaaaaa gagaaaactt
    10981 tggaaagcaa tatctgcggc tttgtcagct gttctgctgg caacttccgt gccttctgca
    11041 gcatttgcgg cagtaccggc aaaaacagca gatgtgcaga caaaagcagc tgatgaaaat
    11101 gagctgaagc tgtggtatga cgagaaagct ccggacagct atgacggatg ggaaaaatgg
    11161 tcccttccgc ttggaaacag cggcatcggt gccagcgtgt tcggtggcat tacccaggag
    11221 cgtatccagt taaatgaaaa atcactctgg tccggcggtc cgtccgcttc cagaacgaac
    11281 tacaatggcg gaaatatcga gacaacaaat gtaaacggtg tacagaccaa aatgagtgag
    11341 gtagtaaagc agatccagga ggcatttgca tccggcaaca ccagcacggc agaatctctc
    11401 tgtgcaaagc tggtcggcgt cagtgatgat gcaggtacga atggttatgg ttattacctg
    11461 agctatggaa atatgtacct ggatttcaaa ggtatcacag aaagttctgt cagcaactac
    11521 aaaagagcac tggatcttca cacagctgtt gcaannnnnn nnnnataatg gcacacacta
    11581 tgtaagagaa aacttcgtca gctatccgga caatgtgctg gtgacaaaac tgacagcaac
    11641 cggcggaagc ggcaaactga acttcgatgt caaggtcaat ccggacaatg aaaagggcgg
    11701 cggaagcaac aaccctggag caaattctta tcagagagac tggaatacaa aagtaaacgg
    11761 cggtgagatc acggttgccg gaactctgaa agataaccag atgaaattca attcccagac
    11821 aaaagtgatc gcagacggaa ccaccacaga cggaaccgat aaagtaacgg ttgcagatgc
    11881 aacaagcgtt acgatcatca cttctattgc aaccgattat aaagataaat atccaaaata
    11941 ccgtaccggt gaaagtgcag aagcactgga taccagagtc ggcgggtatg tagacaaggc
    12001 agcagcgaaa gattacgata cactgaaagc ggatcatgtt aaagattatc agaacatctt
    12061 cgaacgtgta gatctggatc ttggtcagat tccttctgac aagacaacgg atgcactgct
    12121 ttcagcatac aaggcaggta ctgcaacgga agcagaacgc cgctatctgg aagtcatgct
    12181 gttccagtat ggccgttatc tgacgatcga atcttcccgt gagacaccgg aagatgatcc
    12241 ataccgtgag acacttccgt ccaaccttca gggtatctgg gttggtgcga acaactccgc
    12301 atggcatgct gactaccaca tgaacgtaaa tcttcagatg aactactggc cgacctatgt
    12361 caccaatatg gcagaatgtg cagagccatt gatcaactac attgatgcac tgcgtacacc
    12421 tggccgtgtg acagcgaaga tctatgcagg tgttgagagc aaagacggcg aagagaacgg
    12481 tttcatggca catacacaga acaacccgtt tggatggacc tgtccgggat ggaattttga
    12541 ctggggatgg tcaccggcag cggttccgtg gatcctgcag aactgctggg aatactatga
    12601 ctttaccaga gatgccgatt atctgaaaga gaaaatctat ccgatgatga aagaggaagc
    12661 tactctgtat gatcagatcc tgatcaagga tgcagacggc aaactggtat cttccccaag
    12721 ctattcaccg gagcatggac caaagacaag cggtaataca tacgagcaga cactggtatg
    12781 gcagctttat caggacacca tcgaagcagc aggtatcgtt ggtgagacag atacagcaaa
    12841 agtaacacag tggaagaaaa atcagagcga tctgaaagga cctattgaag taggtgacag
    12901 cggtcagatc aaagaatggt ataccgagac aaccgtaaac agcctcggac agggctacaa
    12961 ccacagacat ctttctcata tgctgggact gttcccgggc gacctgattt ctgtagatac
    13021 accggaatgg ctggcagcag caagagtttc catggaaaac cgtgtagata aatcaaccgg
    13081 ctggggcatg ggacagcgaa tcaatacctg ggcaagactt ggcgatggaa accatgcgta
    13141 caaactgatc acagacctgt ttgacggcgg tattcttacc aacctgtggg atacgcattc
    13201 accataccag atcgacggta acttcggaat gacttccggt gtggctgaga tgctgcttca
    13261 gagtaatatg ggttacatca atctgcttcc ggcacttccg gatgcatgga gcgatggttc
    13321 cgtagaaggt ctggtagcaa gaggaaactt cgaactttcc tatgactgga cagacggaaa
    13381 actgaaccag gcacagatcg tttccaacaa gggcggagaa tgtcaggtac agtactccga
    13441 tatcgcaaac gcaaccgtta ccgattctga cggtaaggtt gtagagacaa caaaagtaag
    13501 cggaaaaaca aaccgtgttt cctttaatac agaagaagga aaaacttata cgatcaaagt
    13561 tccggaatct ccgaagaatg taaaagcaaa ctactacgga gagagcggca caaaagtaag
    13621 ctgggctgca gcagaaggtg caacttctta caatgtatac cgcagcgaag acggaacaac
    13681 gtttgaaaag gttgccgaaa agacagaaag aacttctttt gtagataaaa aagcatttac
    13741 agatgtttca gcagtcagct acaaagtgac agcttgttac gaagacaaag aaagtggcga
    13801 ctccaaagtg gcaacagtca aagacctgac acagtcaagt gaaaaactgg cacatgatgg
    13861 ctggaaggca accgcaggaa gtgaagaagg atccggaaat gatggccctg cttcctgggc
    13921 gattgacgga aataccggta caatctggca ctcaagatgg tccagtggcg gaacacatcc
    13981 ggatatcgca aatgatcaga ataatgagtt tacgattgat tttggtcaga atgtaacaat
    14041 caacaaattt gagtatgtac ctcgttcatc cggaacttct gtaaatggta ttattacaaa
    14101 atacaaactg ttatacagta ctacnnnnnn nnnnatccgg aaatgatttc aaagaactga
    14161 caagcggtga gtgggacgca gacaagacgg ttaagacggc aacgtttgca ccgaccgaaa
    14221 tgcgaagaat ccagatccgt gcactggcaa cactggatga taccgcaacc aagaatcagc
    14281 acgtgacagc agcagaattt aatgcataca aatacgttgc aaatacaccg gtcatgacgg
    14341 atacggatgc actctgggat gcagttctgg ctgcacagaa aaaagactta agtgtatata
    14401 ccgatgccag tgtacaggct taccaggcag caatcacagc agcaaaggca aaacttgccc
    14461 tggaagatga tgcagcccag gcggatgtaa acaaagcact tgctgaactg aaagatgcag
    14521 agaacaagct ggaggcaaaa ccagacaaag gtaatctgaa tacggcggtg gatgcagccg
    14581 ctaaaacaga cctgaacggt tatacaaaag agagtgtaga tgcatttaag aaagcactgg
    14641 atgcagcgnn nnnnnnnngc tgaaagatga aaatgcaaca aaagagcaga ttgaaaatgt
    14701 tctggcaaaa ctggatgcgg caaagaaagc tctgaaaaaa gatactacac cggatacgga
    14761 aaaaccgaca ccgacaccgg aaacaaaagt accggctgtt ggaacaaccg ttaccgtaaa
    14821 aggtgtaaaa tatgcagtaa ccaagtccgc agcaaagggc ggaactgcag aggcagtcaa
    14881 agtgaccgga aaaggcaaga agattacgat cgcagcaact gtgaagattg acggtgtgac
    14941 ctttaaggta acttccatca aaaagaatgc agctaagaaa aataagaata tgacaagcgt
    15001 tgtgatcggt aagaatgtaa cttccatcgg agcaaacagc tttgcaaaca gcaaaaaact
    15061 ggcaaatgtg a
//
LOCUS       DAWDWH010000025        14748 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_2299_length_14748_cov_12.136460, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000025 DAWDWH010000000
VERSION     DAWDWH010000025.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 14748)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14748)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14748
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2299_length_14748_cov_12.136460"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1785..1794
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5165..5174
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6605..6614
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11923..11932
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4392 a         2818 c         3759 g         3739 t
ORIGIN      
        1 cctccgtaaa ttttttattt gtctcgaacc atcgctgcaa acttctcatg aatcctggta
       61 tcgttatcca gttccgggtg aaatgcaaat gccatttgat ttttatactg cacaccgaca
      121 attttcccat cgaccgtggc aaggacttcc acgccatctc cggtctctgc gacatatggt
      181 gcacggatga aggtcatcgg gacttctcca acgcctttta cttcagcgac ggtatggaag
      241 cttccaagct gtctgccata ggcattgcgg cgaacgcaca tcgggattgt tccaaagtga
      301 catccttcct ggccttccac ctctgaggca agcaaaatca gcccggcaca ggttgcaagg
      361 accggcatgc cgttttctat ctgttctttt atcgtatcaa acatgcccag ttcttttaaa
      421 agctttccct ggacggtgct ctcgccgccg ggaagaatga tgccgtcata aggtgtttcc
      481 aggtcttttg cctggcgaag ctcgatacag tcgacaccga gacgtttcaa tacattttca
      541 tgttctgcga aagcaccctg gagtgccaaa actgcaacgg tcattatttt cctctttctg
      601 ccatcaataa ggcgatttcc tgttcattga tgccgaccat agcttccccg aggtcttcgg
      661 aaagtttggc gatcagtttt gcatcagtat aattagtaac agcttttacg atcgcagctg
      721 ctctcttttc tggatttccg gatttgaaga taccggaacc tacaaagaca ccttccgcac
      781 cgagctgcat catcagagca gcatcagcag gagtagcgac accgccggct gcgaagttta
      841 caaccggaag tctgccgttg tcatgtacat aggcaaccag ttcataagga acctgcagtg
      901 tttttgcagc ttcaaacagt tcatcttcac gcatactggt cagctttgcg atttcactgt
      961 tcatttttct catgtggcgt acggcctgca cgatatctcc ggttcccggt tcccctttgg
     1021 tacggatcat ggaagcacct tcgttgatac gtcttaaagc ttctccgagg tcttttgcac
     1081 cacagacaaa cggaacacgg aagttgcgtt tgttgatatg ataaacatca tcggcagggg
     1141 agagtacttc actctcatca atgtagtcga tttcgatcgc ttcgagaacc tgagcctcca
     1201 cgaaatgtcc gatacggcat tttgccataa ccgggataga aacagcattc tggatgccct
     1261 ggatcatttt cgggtcgctc atacgggaga cgccgcctgc ggcacggata tcagccggga
     1321 tacgctccag agccatgact gcacatgcac ctgcagcttc agcgatcttt gcctgttccg
     1381 gtgtggtgac gtccatgata acgccgcctt ttaacatctg tgccagttct ttgttcaatt
     1441 tatatctttc atttgttgta ttcatgttta aacctcctgc tttgtttttt gcaatcttta
     1501 ctttttactg gtttcgaatt ataatggaaa agtgactatt ataaaattgc caaaatagat
     1561 atattcagta aggtcagatt ggagaaaata atcagatgct gacatacagt tttacaaata
     1621 cagggtcaga ttcgatgtat gaatatttat ataagtgcat tcgaaacgac ataacacagg
     1681 ggaagattaa ggcaggggag aagctgccgt caaagcgtac ttttgcaaaa aatctgggca
     1741 taagcgtgat cacagtggaa aatgcctatg gacaattatt agcannnnnn nnnnctgagc
     1801 agtgcgacca tggaaaagaa gaaggtcaga aaggaacagc tgacgctgac agggggagcg
     1861 acttcttatt ttgcagattt ttccagcaat cagacggaca gcgagatctt tccgtttacc
     1921 atctggtcaa agacggtgcg ggaagtttta aatgacaatc agaagcagct gatgatcaat
     1981 ccgccctgtg gggggatcct ggagctgcga caggcgattg cccggtattt aaaggagttc
     2041 cgtgccatgc aggtcgagcc ggaacagatc atcatcggag cggggacaga atatctgcat
     2101 ggacttctga tacagcttct tggaaatcac cggatctatg gagtagagaa tccgggctat
     2161 cataaaattg ccaggattta tgaaaatatg aaggtagaat atgccaaagt tacgctggat
     2221 caggatggtg tatccatccg ggaactggaa gaaaaatgcg tggatgtcat tcatacctcg
     2281 ccctcccatc attttccgac cgggatcgtg atgccggtca gcaggcgtta cgagttgctt
     2341 ggctgggcag ccaaatcgcc ggatcactat atcattgaag atgattatga cagcgaactg
     2401 cggctgaccg ggaaaccatt tccgacgctg cagagtattg atgtgtcaga tcgtgttatc
     2461 tatatgaata cgtttacgaa aacgctggca tcaaccgtgc gtatcagcta tatggtgctg
     2521 ccgctgacgc tgacggagat cttttatcag aaactttcgt tctattcctg tactgtttcc
     2581 aattttgaac agtatacatt ggcaaaattc atggaaaacg gcagttttga aaagcatatc
     2641 aaccggctca ggaactacta tcagacaaag agagatgcga ttctgcaagt atttcagaaa
     2701 gagccgcttg gaaaatatgt caccatccgg gaagaagggg ctggggtaca ttttctaatg
     2761 cagatccgta cagaaaaaag cgaggaagag atcgtaacgg aagcaaaggg aaaaggcatc
     2821 aaactggcac cgctgtccca ctattttgat ggtgaaaaag atgggaattt tgaaaacacg
     2881 tacgtgatca attattcttc ggtggatctg acaaatattg aaaaagcagc acagatactg
     2941 ggcaagattg cgggggcata ggagaaaaat ggataaaaat aaagacaaaa agcagactta
     3001 tgtgctgcga gtactgatga tgatatccac tgttgtcctg atcattctgt tttttgccac
     3061 gatgtccatg gtagggaaaa ttcagggaac agcacgcgtg gtcaactatg cagggctggt
     3121 acgtggaaag acgcagcgta tcattaaaag ggaagatgca ggtcaggaag aagatgcgat
     3181 gatagatgat gtggcggcgt gttgtgacgg gctgagaaac ggaagtgatt cgctgaatct
     3241 ggttcgtctc gatgactatg cttttcagag caaaatggag gaactggatg actattttca
     3301 gtctcttgag caggagattt atctggtacg tgaaaaaggc tatgaaaata gcgaaatcat
     3361 cgccaaaagt gaacagtttt tccagatctg tgacgaagcg accggactgg cggaggctta
     3421 ttcgcagaga aaagcatctg ccctgaacat tctggaaaag attgttatgg cggatatcgc
     3481 gattctgatc gggattcttg gcatcgaact tttcaaggct ctgaggtatg ccgcccagaa
     3541 ccggatttta cagaaaaagg tttatctgga tgaagcaacc ggactgccaa acaaaaacaa
     3601 atgcgaagag attttaaatg acgaaacggt tttggatgag aaatatgtga cggcggtctg
     3661 tgtgtttgat ctgaacaatt taaggacgat caacaacaat ctcggacatg ataagggaga
     3721 tgaatatatc cgtagttttg cacagcagat gcgtcttgca gtcccggagc agtattttgc
     3781 cggacgtaac ggtggggatg aatttcttgc agtgctgacg gatgtcaatc atacagaagt
     3841 ggaagaatgc ctggaggcga tacgcaggca gacggaaaaa tattccagac ttcatccggc
     3901 aatgccgatc agctatgcgg cgggatatgc actgtcgagt gattttaagg atgccacgat
     3961 gcggagtttg ttccggcatg cagataaaaa catgtatatt gataaaaacc gtgccaagat
     4021 gcaggaggct gcagaaaagc agaaaatgaa gcgaaagctt ctggaagagg tccgaaagaa
     4081 ggggtaccat ttttcggact gtctttactg cgatgccctg ctggatcagt acgaagtgct
     4141 ccgctccggc gatggatttt ttctggcaca ggacggcaat tattccggtg cggtggagca
     4201 gatcgtgaaa gaattttcaa cagaagagac aagacgccag atctggtcag gacttcagct
     4261 tgcacatatc agtgaaacac tgacaggcga aaaccggaaa acggaaattc tgtactgcaa
     4321 agagctggaa aatggcaagg tgaaaagagg cagactgacc ttattgtttg gcaatgacac
     4381 gggtgaggga aaactgcatc attttctgct ggtatttgag acgttcagcg acagcagaac
     4441 ggaagaaacc aatgaaaagt cacagctgat gcgttattat gagcagatga aacagtccat
     4501 tttggaaaac ggcaattatg tggatgccct gatggagaca gcacaggccg tttatatggt
     4561 tgacctgaca catgaccggc tggaaaaatt ttttgaacat tcaaaagaaa aagaatttaa
     4621 gctggatcag gaaacgccgt gttcttacgg ggaatactgc aaaaagagaa gtgcctttgt
     4681 tacagaagag acactggaaa attaccggat cgtagattca tcggaaaagc tgctgaagcg
     4741 ttttggagaa ggaaccaaac aggtgaccgt agaataccgg gaaaaaggtg tggcagaggt
     4801 tctggtgtgg ctacagaaaa cggttctgat gtcacaggaa ctggtatttg atgagaaaac
     4861 aggacaggaa agtgtggtga tccgcggtat tattctgttc aaaaacacct ctgctttcca
     4921 tgaaaaagaa gaggaagaaa aagaacgttt gcagtccgct ttcgaggcgg ctgattcgga
     4981 gagcaaggcg aagaccgaat ttatgaaccg catgagtcat gatatccgca caccgatcaa
     5041 cggtattatg ggaatgctgc atatcatccg caaaagtgag aaagagccgg aaaaagtaga
     5101 agaatgtctg ggaaaaattg aactttccac cagtcatctt ttggcacttg tcaatgatgt
     5161 gcttnnnnnn nnnntttgat cttgttgagt tgatggatga agtggcaggc gtgatggatg
     5221 cccagatcac agaaaccggg attttacata agaaacatcg taaaaatatc cgccatacgc
     5281 atctgatcgg aaatccgctg cacctgcgtc agatcatgct gaatctgttc agtaatgccg
     5341 tgaaatacaa caagacaggc gggtgtgtag acacttatgc ggaagaaatt tcagacgatg
     5401 gaaccacagc catgtatgaa tttaagattg tggataccgg gattggtatg agtccggcct
     5461 ttgtgaaaga agagcttttc aagccattta cgcaggaaaa atcagcagat gccagaacac
     5521 agtacaaagg caccgggctt ggcatgtcga tcgtgaaagg gctggttgaa aaaatgaacg
     5581 gaacgatccg ggttgagagc gagccagatc agggaacgac gtttgttttc cggatgccgt
     5641 ttcagattga tacgagtgta tccggtcaga tgggtccgga gaaaaaagag gaaaatgtag
     5701 atttttccgg tatgaacatt ctgcttgtag aagataatga gatcaatatg gaaatcgcac
     5761 agttttatct ggaagaggcc gggatagagg ttgatccggc gtgggacgga caggaggcag
     5821 tggagaaatt tgcacagtcg gagccgtatg cttatgatat gattctgatg gatatcatga
     5881 tgccggtgat gggcggactt caggcgacag aaaaaatccg cagcatggag cgtgcggatg
     5941 caaaaaccgt ttccattctg gcaatgactg cacaggctaa tgcagaaagc agcagtgaat
     6001 gtcggaaggc aggaatgaat gattatatcg aaaaaccgat agaggccgaa aaactgtggg
     6061 agattatttc aaaaaatctg accgaaaaaa cgttatgatg ggcagaaaag tgactgcacg
     6121 gagtacagat ccatttgcat ggcatctttt gggcatgata tcatatttat agtagaaata
     6181 tcgtttttcg gggaccgcag aatgttatat cgtatggccg gatattgccg tctgtctaaa
     6241 gaagacgggg aaaacagaat aagtgaaagt atcgaaaatc agaaaaaact gattttggaa
     6301 tatgtggata aagccgggga tatgaagctc gtcaggatgt atattgacga cggatattcc
     6361 gggctttatt tttcgaaccg cccacagttt cagcagatga tggcggatat ttacaaagga
     6421 gagattcagg gagtgatcac aaaagatatt tcaagacttg ggcgtgagca tattgaaaca
     6481 gggaattata ttgagcgtgt gtttccgtca cttggcatcc gttatgttgc gattttagat
     6541 ggtgtggatt cgctctgtca cagcaatgaa gagctggctc agttcaaggc actgctaaat
     6601 gatannnnnn nnnngatatt tcaaagaaga tacggggaac tttccaggta cagaaaaagc
     6661 aggggaaata tatgtccggt tttgcacctt atggctatcg aaaagacccg gctgaccgtc
     6721 atcgctttct gattgatgag gcagcagcgg aaacagtgcg taacatctat actctttatc
     6781 tggaaggcgt ttccgtacaa aagatagcag aacggttgaa cagacagaag attttgccgc
     6841 cgaccgagta taaaagaaaa atccagaaac taagttatgt aaaccgtcag ggaaaaaatc
     6901 aggtgcagaa atggaatgca ccaacgattt ataacatttt gaaaaatcag gtatatacag
     6961 gggcgatggt tcagcacaag acagagaaaa tatcctataa aatcgaaaaa tacagaaaca
     7021 ttccgatgga agaacgttat gtggtggaag gaatgcacga ggcgatcatt tcaaaagaat
     7081 tatttgcaat ggcacaggaa aaacggcaga gatttaaaaa cagaaattcc tgaaaacagt
     7141 tattgacaaa tgggaaaagt atggtaacat atatataaaa taattatata taaaaatttt
     7201 atatatacaa aggggtgagt gtatgtacta ttatccggtg tcagctgttc tgacggaatg
     7261 tctgatcctt tcggtcgtgg aacagcagga ttcctacggt tatgagatca gccagacggt
     7321 gaagctggtt gcggcgatca aggaatcgac gctgtatcct attttgagaa agctggagac
     7381 gggcggatat ctgactactt attccgagga attccagggg cggaaacgaa agtattattc
     7441 catcacggaa gagggacgca ggcagctggc atatctgagg aaagaatggc gggaatatcg
     7501 caatactgta gatgatattg tggaagggag agtgaaaaaa tgacgagaca agaatatatg
     7561 gaagagctga agaaatatct gaagcggctt ccgcaagaag attttgacaa tgccatagag
     7621 tacttttcgg aatattttga agaggcaggt cctgagaagg aacaacaggt tatgagagaa
     7681 cttggtgaac caaaagaggc agcaagagaa ctgtttctca atcttttgca ggagagcgta
     7741 gaaaaggatc aggatattgt ggaagagaaa gcaccggtct taaaaggaaa gagacgttct
     7801 ccgggcaaga tcattcttct tgtattcctg gttctatgtg catcaccggt cagcctggca
     7861 cttctggcat ttgtttttgc agtgctgtgt gcggtgatcc tcactgttgc ggcagtgatt
     7921 ttttcgattg gtattaccgg cattgcggcg atagccggag gtattgtgac catcggggtt
     7981 gggggctctt tgatcctgaa atctgctgca gcagcctgta tgatggtcgg aggtggattc
     8041 ctgatgacgg gaattggaat cctcgcaggt cttttgactg tatatatctg caaatggtgt
     8101 gcaaatggga tcggacgtct ggtcaacaga cttgtaagaa agaaggtgca taagaatgaa
     8161 taagactgca aaaacagcaa tgataacagc tctaacgctc tgtgcggtgg gaatggttct
     8221 ttcaacagca ggatattttg cgggcggaaa ggatttcaac gatagttccg gacatgtata
     8281 tatatccggt ggaacggatt ctgcaaggaa aaatcttgag gttatggaaa agcagcagat
     8341 gaatgctttt acaaaactga atgtagattt tcaaaatttt gacctggata tcagaaaatc
     8401 aggtgatgac tgctattaca tggaataccg actggaaaaa ggcggtaatg aaaatccact
     8461 gacatgggaa aacaaaggtg gtgagcttac gcttcaggaa acttccggcg gaaaaggaag
     8521 ttattatata agttatgatc ttggaaatct gctgcatcat accgagacgc agaaacagga
     8581 agaaatcaat acagtgattc tctatgtacc gcaggatgca cggttttccg aagccaggat
     8641 tcagcttgcc gatggagatc tgactgcaga acagttctca tgcaaaaata tgacagcaaa
     8701 ctttttggat ggagatatga tactggatca gggaaaattt gcagatggag tcattacact
     8761 tgccgatggc gatctgaaga caaatcacag cagcttcagc ggtaatctgg aaataaacaa
     8821 ttcagacgga gatgttttca tacaaatgga aaacggcaat gcagcaaaga tgaatattca
     8881 cctgaaaacc acagatggag atgtgatcac agacaatctg ccgcagggca aatcggaacg
     8941 caaagaagac agctctcttt acgaaaacaa tgtcgatggc accacatcaa cgctgaatgt
     9001 aatatgcagg gacggagata ttactttgat cgaatctgca aaacaataaa agttatctct
     9061 atatggctca catgacggga cttcacttct ggaaacatac atcgacatga tttccggtat
     9121 gtggaagaat gtgatctgtg tgggcaccgg aaatgaaggg aacacaggtg ggcatatttc
     9181 cggaaagctt ggtcagcagg aagagagaat cctggaattc gggatttcgc agcaggagcc
     9241 ggtgatgaac attcagctct ggaaggccta tttcgatcag tttcagatta tattgattca
     9301 tccggatggt gaaagcttcg gaccgcttca ggagcgtctt ggagtgcaga gaatcctggc
     9361 cgggaataca gagattctga tctattacgg agagccgaag ccctacacta ccgctcagga
     9421 gatttatttt gattttatcc caaaaggttc ttatgtcgat gacggcgtct ggaagatccg
     9481 tctgatcccg cagaagatcg tggaaggaaa ttatcatctg tggatgccgt ctgccgctct
     9541 tttaaatcca ttgacgcact ttttttcgcc aacggttgac acgactctga ccattccttc
     9601 aacggcaagg aatgtggttg cagtcggagc ttacaatgca cgccttatga catatgctcc
     9661 gttttccggc cgtggttaca caagaggaaa cacacaagta aagccggata tcgtggcacc
     9721 tggcgtagat atcgtgacaa ccgctgtggg aggttcctac acaggtgtga ccggaacctc
     9781 ttttgccacg ccgtttgtga cagggtcagc ggcgatcatg atgcagtatg gcatcgtaca
     9841 gggaaatgat ccgtttcttt atggagagaa gatccgggca tatctgcaaa gaggtgcaca
     9901 gagattaaca gcacttgtgg gatatccgaa tgagattgtg gggtatgggg cactgtgcgt
     9961 agcggagagt attttatgat atcgttatca gtctgaaaga agaaacgctc gtaaggattt
    10021 gttgaagcta tgggatgggt tggagatgtt tggagtatgg aacaggtgga agaagcaaaa
    10081 gaacatcata ctcttgttta catcacaaga atgtgctaga ataaaccaga attatagaag
    10141 atatgcgaaa cagaaactgc ttcagtattt ttaccggtac tgcttataca gcacgcatcc
    10201 tggaatgtgg cattgttccg ggatacggag attgcagcca atttcctgat tcttggattt
    10261 tcaggtaagt taaaaacaga attacagtaa atgatcaaaa gaaaagatgg catgaaaaat
    10321 atgctgtctt ttctttttta ggatagcagg ggaaaaagcc tgaaaccaaa ttgtgggagt
    10381 aagaaacaat ttgcatttta tcttttcgtt ttcatattga aaaaaccaga aaactagtgt
    10441 actatatagc tatgacagtg atacaagaaa gaaggaagag cttatggaat acaattcatc
    10501 gctgccaatc tatctgcagg tagctaattt gatcaaacgt gatattgtca cagggaaacg
    10561 ggaacttggg gaaaagcttc cctccgtacg ggaactggcg gtgtcttaca ccatcaatcc
    10621 gaatacggtc agccgtgtat acaaagagct tgagacggaa ggtgtctgtt ttaccagacg
    10681 tggcatggga acttttgtga cagaagattc agagaaggtg aaaaatatga aagaagagat
    10741 ggcggggacg ctgatccatg aatttttaga aggaatgcag cagcttggat ttaccaaaac
    10801 ggatgtgatc cggattctga aaaaaaagga agaaagtggt gaatgatatg gaaaatgtac
    10861 tggaaagtat tggagtaacc aaaaactatg ccggtaaggc agcggttgac aatgtatcgt
    10921 taaaactgga gacagggctt gtctatgcga tgatcgggcc gaacggaagc ggcaagacta
    10981 cctggatgaa gatggtggca ggccttgtca aacctggaaa aggggagatt ttatataaag
    11041 gcgaagccat cggaaaagag agcaagaaag agattgccta tatgtccacg gagccatatt
    11101 tttatagctg gatgacggtt gcagatgtcg gcagatatta tcaggacttt tttgaggatt
    11161 tttcaatgga aaaatatcat cagatgctca aaagaatgga gctgacagaa gagctgaaaa
    11221 ccaaaaaact ttccaccggt atgatggcaa agttaaagat cgcagtcaca atggcaagaa
    11281 atgcaaaact gtatctgctg gatgagccat taaacggcat tgatcttctg gcaagggatg
    11341 agatcatgaa aagtattctg gaagtcattg atccggatgt cacacttgtg atctccagcc
    11401 atctggtaga tgaactggag cgagtggcag atgcggccat ctttatgaaa gaaggaaaaa
    11461 tggtcagaca gtgtatggtt gaggaactga gaagcagcga aaaaaaatct gttgttgatc
    11521 tgtatcgtga aatttacgga catgggggag aggaactatg ttaaaattaa tgaagtatga
    11581 attccggaag agtgcatctt ccaagcttat tctgctggtg cttacagcag tttttgaatt
    11641 ggcatttctg gggggcgtat tttttaaagg atataatctg atggggacag gtatgatgtt
    11701 cctgatcttg tgtgcatttt tcggtgtgtt ttacatcgga ctggaaggta tcatgacact
    11761 gtatcgggat ctgaacagca aacaaagtta tatgctgttt ctgacaccaa agagcagtta
    11821 tgagatttta ggtgccaaaa tcctggaaaa tggcatttca atccttatgg caggagcatt
    11881 ttttatcgta cttgccgtgt ttgacctgac tgtggcaacg ctnnnnnnnn nnactgaaca
    11941 tcaagatagt atcagatgca gtgatcctgt ccgtctttgc atccctggtc agctggatca
    12001 gtattgttgt gatcgccgat atggcagtga ttctttcggc gagcgtgctt gcaggcaaaa
    12061 agggaagcgg gatcgcggca tttgcactgt ttctgatcct ttcctggctg ttcagtcaga
    12121 tgctgaacct ggtgcctgca tttgacaata tgaacctgac catgattctg gaaatcgcag
    12181 tatctctggt actgagtgtg attttctact ttatcagcgg atggatcatg gaaaagaaac
    12241 tgagcgtctg aaacagaaaa atgataggtt gacctttatc tcctggcatc gtagaatagt
    12301 aacagagcaa aaagctttct attgatattg agaatctgac aggagaaaaa gatatgttat
    12361 catttgaaaa tgattattct gaaggagcac atgaaaagat ccttcagaga tttatggaaa
    12421 caaatctgga gaaagtatcc ggatacggat ctgatccata ctgtgaaagt gcaaaacaga
    12481 aaatccggga ggcgtgcgaa tgtccgcagg cagatgttta tttcctgacg ggcggtacgc
    12541 agacaaatgc cgttgtgatc agctcaatgc tggaatctta tgagggcgtg gtcgcagcac
    12601 agaccggcca tgtagcctgc catgaggcgg gagccattga gtacagcggc cataaagtga
    12661 tcacagttcc gcagtacaaa ggcaaaatga aagcagaaga tctgaagaac tgcctgaaaa
    12721 cattctggga tgacgaaagc cacgaacata tggtatttcc ggggatggtt tatatttccc
    12781 atcctacgga gtacggtact ttgtatacaa aggcggagct ggaagagatc agcagcgtat
    12841 gccgcagtta taatatccca ctgtttctcg acggagcaag actgggctat ggtctgatga
    12901 gtgaagaaac agatgtaact cttccgatga ttgcaaaata ctgcgatgtt ttctatatcg
    12961 gcgggacaaa agtcggagca ctgtgtggag aagccgtcgt attttcgggc aacagaacac
    13021 cgaaacattt cctgacgctg atcaaacagc ggggagcact gcttgcaaaa ggcagattgc
    13081 tcggaatcca gtttgacaca ctgttcacag ataacctgta ctttgaaatc agcagacatg
    13141 cgatagcaat ggcagattat ttaaagaaag gactggcaga aaaaggctgc cgtttctatc
    13201 tggattcacc gaccaatcag cagtttgtca ttctcgaaaa ccggaaaatg acagaactaa
    13261 aaaaagaagt aaagttcagt ttctgggaaa aatacgatga aaaccacacc gtagtacgct
    13321 ttgcgacaag ctgggcaacg aaaaaagaag agatcgatca gctgctggat ctgttctgag
    13381 attttagcag cacacaaagg cagggtaagc gattaccctg ctttttcaca actctttcac
    13441 aaaatcggct gttttctttc agcttgtttt aaactttacg ggatatactt ctcttaaatc
    13501 aaagagaaaa ggaagtgcat gccaagtctc acgtgcgttc tatctatatc tcacccggta
    13561 tatccacgac actgtctctg acagccagtg tcggcggcag tacgatctta taaggcagat
    13621 gctgcggatc atccaggcgg tcaagcaaca ttttcactgc catttctccc atgacttcca
    13681 gatgtgcggt gacactggtc agcggtggct cggtcaggat agacagcggt gtgtcattga
    13741 aactgatgac agatacatcc tccggaatgc gaagtccgac ttcacgcagg ttgctgacag
    13801 cggtgatcgc agattcctca ttataggcaa ggagagctgt cgggatatga tctttttgtt
    13861 ccagccattt gcggatctga gcagaaccgt ctttggcaga aaggccggta tcaatgatca
    13921 gatgctcgtc gtaaagaccg gcatttttca tataatcaat aaaatactga cgtcttgcct
    13981 ccagggcagg gcgttttttc tggtctaatt tataggcagg acctaagaaa ccaatcttgc
    14041 ggtgtccctt gtaaagcaga taatccagtg cctgctctac gccgagacgg aagtttaaga
    14101 caaccgaatc aaagcggcgt tcatccgggg aagaatccac aaatacgatg ttcgggctga
    14161 tctccagcaa ctgtgcaatc tgctggtctg aaaaaatgcc gatcgccagg ataccgtcaa
    14221 gcggggtctt ttccagtgtc tgatatttgc cagactgctc aaaaagataa gatacattac
    14281 agtcatgctc catacagttg cgtaccacat aattcttcag ataaagatag tacggatcag
    14341 ccagctgctc ttttaaggac atcatctcaa cgatggcgat atggacgagg cggtttttat
    14401 gggaacgtct tgttttcgga agcgtgtagt gaagttcatc tgccgcttca agaattgaga
    14461 ctctggtgtt gtcggttacg ttcaaagtcg gatcattgtt cagtacacgg gaaattgtgg
    14521 tcactgagaa tccggtgtgt tttgctaaat ctcggatagt tgccatgcta ttttctccaa
    14581 tacatttttt atttgcacat aactgccgaa aaacggacca aaatcgcaca tcaggcctta
    14641 cgtgtgtctg gcttgaatgt tatagttatg ttcactcatt tgtttacttt aatatctact
    14701 acaatatatt tatggttaat atcccttaca gccagtaagg aattatcc
//
LOCUS       DAWDWH010000026        14402 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_2357_length_14402_cov_11.037220, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000026 DAWDWH010000000
VERSION     DAWDWH010000026.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 14402)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14402)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14402
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2357_length_14402_cov_11.037220"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1875..1884
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5296..5305
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5964..5973
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10619..10628
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4198 a         2988 c         3830 g         3346 t
ORIGIN      
        1 ggggggaatt atggcaaagt ttaatctgga ttattacagc ggagagaatt tttactccga
       61 cggagatatt gaaaaggaaa tactgaagat cgtcaaaaca cagggcagtt acgagagtat
      121 ggagaatgtt ccgtttccgg tactttacca tctttccaga gtccgcgaga acatcctgaa
      181 ctggtatccg tttcaggagg atgcgacctg tctggagatt ggctccggct gcggggcgat
      241 cagcggcctg ctctgtgagc ggatgaaaaa ggtcgtgtcg gtggagcttt ccaagcagag
      301 agcggacatc aacatggcaa ggcatgagcg tgtgccgaat ctggagatct gggtcggcaa
      361 tttaaatgat atggtgttcg gtgagcagtt tgattacatc gtgctcaacg gcgtgtttga
      421 gtatgcaccg ggctttaccg agggggatca gccgtgcgag acgtttctta agaatatcaa
      481 acggctgctc aagccggagg ggatcctgct gattgcaatt gagaaccgct ttggactcaa
      541 gtattttgcg ggtgcaccgg aagatcatac caacggctat tttgacggca ttgccgggta
      601 tccggacaat cattccgtgc ggacattctc caaaggagag tgggaacgtc tgatggaagc
      661 ctgtggtttt tcttatcacc gtttttatta tccgtatccg gattacaagt ttccgcggga
      721 agtctttacg gatgagtctt taaaagagca gaaatatggt ctgccgacct ggaactttac
      781 caagtaccgc atggcactgt tccgggaggg acaggtggcg gagacgattc agcaggatgg
      841 cagaatggat tattttgcca attcttttct gattgagatg agcagaaatc agatccgtgc
      901 tgataaaaaa gtgctttatg cgaagatgag caccgaccgc gaccgtcatt tctccattgc
      961 taccacgatt gaagagcaaa acggagaaaa agtggttgtc aagcagccga tgacaaacga
     1021 ggcaaaacgt cacctgcaga atatgcagaa caaacagaaa gattacggaa gctggagcag
     1081 ccttggcgtg aaagcaaaag gcgatgctgt cgtgacaccg tttcttcagg aaaaaagcct
     1141 tggacaacag gcaaagcagg ccatttatga acataacgtg gaaaaagtga aaaatttgat
     1201 ttccacggtc agtatgctgt gtgaaaaaga atccgcagca actgggaacc gtcatatcgt
     1261 aagccgggag atgtccggaa gagagcggac ggagtttgcc caggtttttg gaacttcaca
     1321 gatctgcccg gaactgccgt gcattgcccc ggcgaacatc gatctgatcc tcgacaatat
     1381 tttcgaaaaa gacggaaaat actttgtcat tgactgcgaa tggatctttg attttccggt
     1441 tccggtggca tttatcatct ggcgtgccat caatgagctg tacagcagct atccacagct
     1501 ggaacaagac tgcaagatgc aggagctttt agaagaatat cagattacgc aggagatgtc
     1561 cgagactttc cacaaatggg gcacctattt tgcagaacac tacgtcggag cgaaccgcgt
     1621 attgcactac agcatcccgg agatcggtat ttctctggaa gagttccgca aacgccacca
     1681 ggagaaggat ctgctgaact gccagctctt tgtggacacc ggaaacgggt tccgggaaga
     1741 ggagaaaatc caggcggaaa cggttctgca ggacggagcg ttccgcgtga cttttgatct
     1801 gaaaaatttc aaggactgga aggcactgcg ttttgatccg ctggaaggaa aaccatgtat
     1861 ctgccggatc gaccnnnnnn nnnngatcga ccatgccgga accaatgcga agttaaaagc
     1921 ggtcaacgct tccggaaaag tggagcatgg cgatctgttt ttaacgaccg atccggttta
     1981 tctggtcaaa atggaagaaa acaaggatca ggtaaagatc agcggcatga ttgcggtact
     2041 ctccatggaa gaggcactgg agcgtgcaaa ctggctgctt ggtaaaaaaa atggtcttgc
     2101 attttggagg aaataaatga aaaaaaggac agaggcagta gatattatta taccggtgta
     2161 caatggttac gatgatttgc agctttgtat gccgagtgtg ttaaagcata cagatcttac
     2221 gaagcaccgg ctgatcctga tcaacgactg cagtccggat gaacggatcg caccttatct
     2281 ggatagcttt gccggggaac atattctggt gctgcacaac gaaaagaata tggggttttc
     2341 tgcgaacgtc aataagggaa tggcacagtc agaggatcgt gatgtccttc ttttaaattc
     2401 cgatacgatc gttaccgcag ggtggctgga caagatcatc gcctgtgcat accgggagga
     2461 gagcatcgga acggtgacac cgctctcaaa cagtgctact ttatgttcgg ttccggtcat
     2521 gtgccaggac aatccgatgc cggaaaatgt cacggcagat gaatatgccg gactgattga
     2581 aacgtgcagc ctcagacgtt atccaaggat caccgttgcg gttggtttct gtatgtatat
     2641 caaacgcagt gtgatcgatg acatcggtgt ctttgacgct gaaacgttcg gcagaggcta
     2701 cggggaggaa aatgatttct gcaaccgtgc agagcaggca ggttatcatc atgtaatgtg
     2761 cgatgacacc tttgtatacc ataaagggac cgcttcgttt gacaccgaag aaaagaaaaa
     2821 gcttcttgaa gaacatgagg caatcttaaa tgaccgttac gctgcccaga tgcggatgaa
     2881 ccatctgtac tgtatggaaa atccggatca ggagatccgc gacaatatca atatgtatac
     2941 aaagctgcac aacggaaagc agaacattct ctatctgctg catctggatt tccaggaagg
     3001 ggcattcaac aacatcggcg gcacacagat tcatgtcaaa gagctgacga tggcactgcg
     3061 ggatgaatac aatgtcttcg tggcggcaag agatgaagaa tatttgagac tgacgatcta
     3121 cacggacaaa gatgtggtct ccctgaaatt tgaaattggc gaagcaccga agtttccggt
     3181 tttccgtgat gaaaagatgc gaaagatcta tgaacagatc ctgctggcat ttgacattga
     3241 tatggtgcat atccagcatt cgcaggatct ttctctggag ctttattttg cggcagagcg
     3301 tttcggaatc ccggtgatcg caaccattca tgatttctat tatgcctgcc cgacgatcat
     3361 tcttctggat aacgaaaatc agttctgtca gaaagaggat gcaaagcttt cctttgaaga
     3421 gaggcagaaa cgctgccggg aatgtctgcg tcagaaaaaa gacattgcaa gccaggttga
     3481 ttatcttcgc atctggagaa gggaaaataa aaaggcactt tccatttgcc ggaaactgat
     3541 cttcccatcc gaaagtgcga gggatattct gcttgaatat ttcccttctc tgaaagaaaa
     3601 atgcatggtc atcgaacacg gagaagacaa actggagcgt gatatcatcc atgtgccgtc
     3661 accggagaag catcagaaga gcgagcacct gcacgtgaag ctggatcgtg tgccgggagc
     3721 cgatcagggg ctcaatgaca tcaaaggctg ggcttatctg gaaggcgtgc caaacgaaga
     3781 gacgaagatt tatgtggaat ttacggacag caaaggcaaa aaggactgtt ttctggcaaa
     3841 caaagtacca agaccggata ttgtcgatgc atttggtgca acagaagcac tgatgtgcgg
     3901 tctggatctt cgtatcagta ccagaaaact tgccgacgga ccggtgaaga tccgtgtcta
     3961 tgtcaaacat gacggcgtgt tctacacgga taatcaaagt accaaaggag aatatcacca
     4021 tcgcttcgga aaacgaggaa ggctcaatgt ggcattcctt ggaggaatgg ttccgcagaa
     4081 aggaagtctg atggcaagag agctgattcc gatggacaaa ggcgggatca actggtttgt
     4141 actgggtgcg atcggtgata agaatattct ggaaatttca caggagaact gtttcttcag
     4201 ctccacttac aaaaaagagg aacttccgca gcttcttgaa gataatgaga ttgatattat
     4261 ctgcattctg ccgatctggc cggagacatt cagttatacc gtctcagaag catggcttaa
     4321 tgggattccg atcgttgcaa ccgacatcgg agcagtcggg gagcgtatca gaaagacagg
     4381 cggcggctgg ctggtgcagc cggatgccag cccggatgag gtgatgcagc tgttgcatca
     4441 tatcatcgat catccggaag aatatcaggc aaagaaagag atcgtggatg atatggagat
     4501 gaaaacggtg gagcagatgt gcgaagagta ccgcagcttc taccgggaac ttctggagtt
     4561 tacggaaaaa gaactgccgg aagacaagat cgaccgggat ttcattttcc agggactggc
     4621 tcttggcgat ccatccattg gcggcagcgg cagcatcgcg gcgatgaacc gactgaaaaa
     4681 cgagaatgcg gcactgaaag cgtcgatcga agtaacaaaa ggaacgattt cttataaaat
     4741 ggcaaggaaa atttcggatg cgaaaatacc atttaaagag cagatgaaac gattctttca
     4801 tcgaaaatag agcagcagaa aatgagcagt cggagacccg gtggtttccg gctgcttttt
     4861 atagaaaatg ctcacttccg gtaaaatgca aaggtgtgtt ctcctgagcc gaccggaaca
     4921 cagacgaagc aggcatcggc acgtaaaaca tcggtcttga tgccgtctac ataagctttt
     4981 acattcttgc tgtatgggat tggcagaaag agaaaaccgg cattttctgt cgtcacggta
     5041 ccggagatct gctccttttc ggtattttca tcgaaagcaa tctgagcacc ggtgtaggcg
     5101 gatatggttt tgaaggagcc aagcagtgca aggtctgtga tccgacagct ttccttttca
     5161 caggaaaagt ttaccgagtc ggacgagatg gaaatggcga tctgcacgtt tttcttctcg
     5221 ccggctgtaa acgggatctg atataccatg tccgaattct gtgagacggt cagcgtgccg
     5281 ccagaatcag ctgtcnnnnn nnnnnagtaa gataaactcg ctggtaaagt cttgatgtgg
     5341 cggtatcaag ctggatagct gccggagagg aaggatttac cgtaagatcc gtaaagtcgc
     5401 gggaaatgtc gtccaaggtg cagctttcct gaagttttga tgccggacgt atcttctttc
     5461 cggtgatctc ttttgcaagg gaagcggtcg cactttccga atccgcagcg tcggaatcag
     5521 cagtatcggc atcctccgat ccttcccctt ccagcagaac attctcctga aggatccgct
     5581 ccagatcgag ggagacatcg ctgtttaaga tggaggtatc tgccgcacgc gtataaaaac
     5641 gtgcaaaaga gggcgggtcc tgactctggg cggaagcggc atcgccgaac tgctcactgt
     5701 aataggaaac ggcggtatcg taaaaagaat ctgcttctcc aaaagagatc agacccatgt
     5761 ggcgtgcggc cacaaccaga agcaggcaga gtaggccgcc aagcagtatg ccgaaaaaaa
     5821 gcgagagcaa atgtccgagg actttgttct ttgctttttt tgtgttttta tccgaactgg
     5881 aatctgtttt atctgaaatc atcgttttca ctttgtcctt tctatatgat aagcgtctca
     5941 ataactggaa atctttatga gtcnnnnnnn nnnatcacgc aaacattcga aattcgctca
     6001 tttttttaca aaaaatggct gctttctcat gttttagcgg tcctcataga cacaaatacg
     6061 tatgcaagca tccgttttgt gtccatttgt cccttgactc atcatatcac atctgccgga
     6121 aatcctgcaa tcaaaaatgc gggagaaaat ccgacacttg acagaaaagc ttcctctata
     6181 ctatagtgta caaagagtct tttcaggtga aacagagaaa aagttaagat aaatgctctg
     6241 gatggaaatg ttttccggaa gagcagaaaa gtaagagagg aaacgtatgg atttttcgaa
     6301 actggcaaaa agcctgaatc cctacacgat caaaaaggga ttttcttatt tgaagcagta
     6361 tggttttcag gaatttaaag tgcgattatc agagcgattt gaaaaagaag aagtcgatta
     6421 taatgagtgg tgtaagaatg atattctgac ggatgaggag cgaaaagcac agagaggacg
     6481 gatctgggag tatccgccgc tgatcagtgt tgtggttccg atttacaaaa caccggaagt
     6541 ttttctgcgg cagatgatcg aatcggtgac ggcacagacc tatccgcact ggcagctgtg
     6601 cattgcagac ggaagtggag ataccgcaga gacaaaacgg atcgttgaca gttatctttc
     6661 tgataaacgt atcaaatata agatccttgc ggaaaatctg ggcatcgcag acaacacgaa
     6721 tgcagcgatg gaactggcgg acggcgatta tattgctctg tttgaccatg atgatctgct
     6781 ttctgaaaat gccctctatg aagtggcgga tcggatctgc aaaacagggg cggagattgt
     6841 ttacacagat gaggacaagg tgacaagtga tctttccgag cgttatcagc caaatttcaa
     6901 gccggatttc aatcttgatc tgctgcgttc caacaattat atctgccatc tgctggttgt
     6961 gaaaaagaca ctggtgcagg aagtgggcgg ccagaaaaaa gaatttgacg gagcacagga
     7021 tcacgagttt ttgttccgtt gtgttgaaaa agcaaaagga atcgagcata ttccgaaggt
     7081 gctgtaccac tggcgggtgc acaaggcttc cacagcggac aatccgctca gcaaaaagta
     7141 tgcctacgat gccggaaaac gggcagtgac agagcacatc cgccgctgcg gggaagaggc
     7201 agaagtgact gatacgctgt tccctggatt ttaccgggcg aaatatcagg tgacgggcga
     7261 accgctcgtc tcgatcatca ttccgaacaa ggatgaaaaa gagacactca aaaagtgcct
     7321 ggattccatc aaagagaaat caacctatcg taattatgag atcattattg tggagaataa
     7381 cagcaccggg caggagattt ttgattatta caaagagatt gacggcaggg acggcatccg
     7441 ggtcgtttac tggaagtcgg gctttaatta ttctgctctg aacaatttcg ggtttacatt
     7501 tgcaaaaggc gattatattc tctgcctgaa taacgatgtg acggtgatca caccggactg
     7561 gctggagcgg atgatcggac agtgtcagcg aaaagaggtc ggcattgtgg gagtgaaact
     7621 gtattatccg gatgatacga tccagcatgc gggcgtgatc atcggtatcg gcggtgttgc
     7681 cggggcgatg tttgtcggta tggcaagaga acgcagcggt tatcttagaa aagcaatcct
     7741 tcagcaggat ttaagtgccg tgacggctgc ctgcatgatg gtggaccgca aagcatggga
     7801 gggggcaggc ggattcaacg aagaccttgc cgttgcattc aatgacattg atttctgcct
     7861 gaaggtaaga agagaaggct atcttgtagt ttatgagccg aatgtcgagc tgtatcacta
     7921 tgaatccaag agccgcggct acgaagacac accggaaaaa cagaaacgtt tcttaagtga
     7981 aattaactat atgaaagcac actggagtga gatccttacg aagggcgatc cttattacaa
     8041 cgagaatttc tcactcaaca cctgcaatta tacactgaga aagcgttagg aaggaatgca
     8101 tatggcagaa gttacggtag tgataccgaa ttataatgga atcggatatt taaaggactg
     8161 tctggataca ttaagagagc agacctgtaa aaattatgag acagtccttg tggataatgg
     8221 ttcgacggac ggaagccttg cgtttgtaaa agaacattat ccgtgggtaa agatcctggc
     8281 acttgaccgg aattatggtt tttgccatgc ggtcaatgtg ggaatccggg cagcagagtc
     8341 ttcttatgta tttcttctga acaatgacac aaagctgcat gaagactgca ttgaggagct
     8401 gttgatcatg atgcggcagc atccgcactg cttttcctgc tcgtcaagaa tgatcaaaat
     8461 gcacgagccg gacaaactgg atgatgccgg ggattattac tgtgcccttg gatgggcata
     8521 tgcacttgga aaagacaggc cgtggaaaga ttatctcaag ccgagaaaag tgtttgcagc
     8581 ctgtggcgga gcttccatgt accgcagaag cgtatttgag aagatcggac ggtttgatga
     8641 ggcacatttt gcctatcttg aggatattga tgtcggctac cgtgcccaga tctatggcta
     8701 tgagaactgg tactgtccgc gggcagtcgt atggcatgtc ggaagcggga cgagcggatc
     8761 aagatacaat gagtttaaag tacgccattc ttcgagaaac aatgtttata tgatctataa
     8821 gaatatgccg tttctgcaga tcatactgaa tctgccgctt ctgattcccg ggtttgtggt
     8881 caagtggctg ttctttgtcc ggaaaggctt tgggaaagaa tatgcaaacg gtatttttaa
     8941 cggcattgcc atgtcagaca aagagaaaaa agttccgttt cagaagcggc atctgtggaa
     9001 ttatgtgaga attcaggtgc agctctgggt gaatgtcgtt cgaagatttg tgatgtaata
     9061 aagataaaaa atagaaatac aaaacgtaaa ttatttgtaa aacgctgcca aaacgggcaa
     9121 aaaagaattg ccctttttcg gcgtatgcgt tatgataagg caagcaaaca gggagaggat
     9181 aaccatgatc aaagacaatc aaacgcttct gaaccgcatc catgtggtgg tagacgcact
     9241 ggtgatcgca tgtacctata tcgcagcctg gtttattcag tttgagtttt tcgcaccgga
     9301 tgttgccgga aaacttccgc ttgagacgta tatgtcagcc ctggtcattc tgataccggg
     9361 gctgctgttg ctctatcatg cattcagcct gtacacgcca aaacgggtgc agggacgaag
     9421 acttgagttt ggcaatatcg taaaggcaaa caccatagca ctgctgctgt tttttacgat
     9481 tgcatatatg cagcatgaaa tcgacttttc cagaaagatg ctctgcattt tctatgcact
     9541 gaacatcgtt gcggaaacgc tggtgcggaa tctgatccgc atggtgttga tgagccttcg
     9601 cagacgcggg ctgaaccaga aacagatcct gcttgtcggc tacagccggg cggcagagca
     9661 gtatatcgac cgtatcattg cgaatccgca gtggggttac cgtgtccggg gcatcttaga
     9721 tgacaatgtg gcacgcggga cgatgtacaa aggcatcaaa gtgatcggaa ggatcgagaa
     9781 tctggtcgtg atcctgccgg aaaactgtct ggatgagatt gcgatcacgt tgggacttgg
     9841 ggaatacgag aaactgcagc acatcgttgc actgtgcgaa aaatccggag tgcacaccaa
     9901 atttatcccg gactacaaca acatcattcc gaccagacct tacaccgagg atttgctcgg
     9961 acttccggtg atcaacatcc gtcatgtgcc gctgaccaat actttcaaca gcatggtcaa
    10021 acgcagcatc gatattttcg gggcactggt ggccattatc ctgttttcac cgatcatgct
    10081 gatttctgca atcctggtga aaacaacttc gaaaggaccg ctgatcttca ggcaggaacg
    10141 tgtcggactg cacaacaagc cgttctatat gtataaattc cgctcgatgg cggtgcagga
    10201 gataaaggat gaaaaggcag cctggacgac aaagaacgat ccgagagtga caggcatcgg
    10261 caagatcatg cgaaagacga gtattgatga actgccgcag ctgttcaacg tcctgaaagg
    10321 agatatgagt ctggtcggtc cgagaccgga gcggcctcag tttgtggaga agttccggga
    10381 ggagatccca cgttatatgg taaaacatca ggtgcgtccg gggatgaccg gatgggcaca
    10441 gataaacgga taccggggag atacatccat caaacgcagg atcgagtttg acctgtacta
    10501 tatagagaac tggaccgtgg gactggatat caagattttg attcttacaa tttttaaggg
    10561 ctttatcaac aagaatgcat actagaaggg ggatttgaga tggcaagaaa aagcaaaann
    10621 nnnnnnnngt atgaccgacg ttacgagcag ggataccgtc aggacggata cggaagagac
    10681 ggcggctacc gtcagggtta tccgggcggc ggctatgacg accggtacta tgaacagaat
    10741 gacagaagaa aacgtgcagc aaagaaaaag aagagaaaac ggatcacgtt tatcattgaa
    10801 atcctgatcc ttgtacttct ggcagccgga ctgtttgttg ctgcaaaggt cggcaagatc
    10861 aaacgtgccg atgtatcaaa aggtgacatt gtgatcaatg acaatgtcaa cctgaaaggc
    10921 tatacaaaca ttgcactgtt cggtgtggac tccagagagg gaagccttga caaagaggct
    10981 cacagtgata ccattctgat cgcaagcatc aacaacaaga caaaagatgt caaactggtg
    11041 tctgtttacc gcgatactta cctggacaat acaaacggag agtaccgcaa ggcgaccgaa
    11101 tgttattatt tcggcggacc ttcccgtgcg atcagcatgc tgaacaaaaa cctggatctg
    11161 gatatcacag attttgtcac cgtagatttc acggcagttg ccgatgtggt cgatgcactt
    11221 ggcggcattg acatcgatgt gcaggaggac gagattgtac atctgaacaa ttatcaggta
    11281 gagggttccc aggtgaccgg caaagagatc gttccggtcg aatatgcagg acttcagacc
    11341 ttaaacggac ttcaggcact gtcttactgc cgtatccgtt atacggaagg aagcgatttc
    11401 aaacgtaccg aaagacagcg taccgttctt cagaagatcc tggagaaggc aaaaagtgca
    11461 gaccttctga cactgaacaa cctgatcgac aacgtggccg gtaatatgat gacaagcctt
    11521 ggcaatacag agctgctttc cattgcaaaa gatgttgctt cctataatct gacagaccag
    11581 acgggatttc cgtttaattt aacggcagca aatcttcagg caggagactg cgtggtggca
    11641 gtcgatctgg cagacaatgt acgtcagctt caccagtggc tgttcgatga cagcagttat
    11701 gaaccatccg attccgtgca ggagatcagc tggcagatca tcaacgatac aggaatacag
    11761 taaacaagaa agtagggaga atgtgaaaaa aacggtagat gttgtgattc cgagctataa
    11821 accggatcat aaatttgacc ggctgatgca tatgcttcag aaacagactt atccgatcgg
    11881 gacgatcctg atcgtaaata cggaagaaaa gttttttccg gaaaaaggat atgaaacgtg
    11941 gcagaatgta cagatccggc atatagagac agaagatttt gaccacggcg gtaccagaga
    12001 tggtgccgcc tccctgcttg acggggatct gatcttattt atgacgcagg atgccgtccc
    12061 ggcagatgtt tatctggtgg aaaaactggt atccgctttt gaaaaggaaa aggtggcggc
    12121 agcctatgca agacagctgc cggacaaaga ctgcgatatc atcgaacgtt aaaacaaaag
    12181 cagacttaga acgtctgggg atcaaaacgt ttttctgttc gaatgtctgt gcgatgtacc
    12241 gcagaagcat ttacgaaaag ctgggaggtt tcgtaaagta caccattttc aatgaagata
    12301 tgatatttgc gggaaaactg atccaggaag gttatgccat tgcctatgtt gcagaagcaa
    12361 aagtggttca ttcccacaac tatacaaatc gccagcagct gcaccggaac tttgatctgg
    12421 cagtgtccca ggcggatcac ccggagattt ttgccatggc aaaatcagaa agtgaaggga
    12481 ttcgcatggt gaaggcgacc gcagcgtatc tgatctcaca gagaaagccg tatctcattc
    12541 cggttctggg gatgcgaagt gcaagcaaac tgctcggtta taggcttggc aggtcctatc
    12601 agaaactgcc ggaaaaaata gttttacacc ttacaggaaa taagagttat tgggagaaga
    12661 aaaagaaact tcagtcaaga tgtaaaaatt aagcgatttt gtcacacttt gaacacaaac
    12721 gactgctata ctttcatacc atacagaaaa aatgtggcct gatagaaaag tcagacgcaa
    12781 aatagcgata agaggaggat ttggtcatgt atgatgaatt tgataataaa gaaaatgaag
    12841 accgctacga tccaaagcag ccatccgcag aggatgaagt gaaaaccggg cagatgtacg
    12901 gggaagaaga tcagctgtca gaaaacgaag aggtacaggc atcgacagcg gaagaaaccg
    12961 aagcatccgc agaatccggg gagcagtcat ctgtttacca ttacagctac gaaagaggcg
    13021 aacaggcaga gccgacaggc agcacacaga actcacagca gtatggttca tcagagcatc
    13081 atgcagaaga gcatattaac gaagaaaaga aaggataccg tcagtactat cagaatggcg
    13141 agtatcacca gagagaagac ggatggcatg acggcgttta ccaaaatcat ccgcagcaga
    13201 aacattcgct ggaaagcggc gggaaaaagc cgtttggcac aggcaagaag atcgcagttg
    13261 ctttaggatg tgcagccgta ttcggtctgg tgcttggtgt gacgttcgga acgatcgatt
    13321 ccaaaaaatc cgtgagcagt cttgtggcag aaagcgagac tatctcatcc ggtgagactg
    13381 agcagagagc agagctgcag acacaggcag ccggtgatgc agataacagc cagagtgtca
    13441 cacagagcgt ctcagcagcc ggcgatgttt ctgagatcgc agagaatgcg atgccatcca
    13501 tcgtagcaat cacaaacaaa ggcgtagaag aagtacagag catgtttttc ggaacaacac
    13561 agtatcagga aacagaaagt gccggttccg gtgtgatcat tgccaagaca gataaagagc
    13621 ttctgatcgc taccaacaac catgtggtgg acggagcaga agaactgagc gtctgcttta
    13681 ccgtggatac ggaaaacacc gatgatgccg tcgtaaaagc acaggtaaaa ggtacggatt
    13741 cggaccatga cctggccgtt gtggcagtca gcctttctga tatttccgaa gatgtacaga
    13801 gcaaactgaa agttgctgtt atgggtgact ccgataaact gaaaatcggc aatcaagtga
    13861 tcgcgatcgg taatgcactc ggatacggac agtctctgac ggtaggttac atcagtgccc
    13921 tgaaccggga agtgacgatc gataatgtca gcaacaatat gatccagacg gatgcagcaa
    13981 tcaactttgg taacagcggc ggtgcactgc tcgatgcgaa cggcaatctg atcggtatca
    14041 attccgcaaa ggcagcagat accggcgtgg agggtatggg atatgcgatc ccgatcaata
    14101 cagcttctcc gatcctggat gagctgatga accgtacgac cagagcaaaa gtaacggaca
    14161 gcgaagaact tggtttcctc ggtgttgatg caagagatgt atccgaagaa gcacgtcaga
    14221 tctacaatct gccggcaggt gcatttgtat acagcgtagt agaaggttct ccggcagaaa
    14281 aagcaggcat caagaaaggt gacatcatca caaaactgga tggcatcacg atttcttcca
    14341 gaaatgaact gttttcacgc atggagtatt acaaagcagg tgagacgatc gatgtggttg
    14401 tc
//
LOCUS       DAWDWH010000027        14371 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_2360_length_14371_cov_13.300154, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000027 DAWDWH010000000
VERSION     DAWDWH010000027.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 14371)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14371)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14371
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2360_length_14371_cov_13.300154"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    488..497
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2392..2401
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4817..4826
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3165 a         3572 c         3180 g         4424 t
ORIGIN      
        1 ctgtcaatct ttttgtttcc acttatttta aaaaaagaaa aaaatgccgg atacgcaaag
       61 tctccataac gattttatgc ttcatcatgc atttcatctg ttcttggact tttgtatccg
      121 gcattttgca atgctcaggg aattatttct gtacagagta taaaatgatc cctgtaatca
      181 tctttttgtt tagatatttt tcacggttgc gatatcgatc agggtcagtt tcttgatatc
      241 ggtgttctcc aggctggtca cgagtgccct ggcggtatcc atagcggtca ggacgttaac
      301 gcctgtctcg atggctgtcc tgcggatgag gaatccgtct ctggagtgct ctacgccctg
      361 ggacggggta tcgatgacaa ggtcgatctt gtggccgagg atcaggtcga ggaggttcgg
      421 tgactcctgc tcgatcttgt tggtgcggat cgcatttacg cctgcttcct gcaggacttt
      481 tgcggtgnnn nnnnnnnccg aggcattctc cggtggactt catttccggg ccgaggctga
      541 tgtcagcacc gcggatcttc tcaaaggaga ataccggcat tttgatcgcg atatagtctg
      601 cttctggtgc aagtccggtc ggatagccga gttcttttaa ttttttgccg aggatcactc
      661 tggttgccag cggtacgatc gggattccgg tcactttgct gatgtacggt acggtacggc
      721 tggaacgtgg gtttacttcg atgacatata cgtcttcgcc gcagacgatg aactggatgt
      781 tgatcagtcc gatgacatgc agggactgtg ccagttttct ggtgtagtct acgattgttg
      841 cttttacgtg gtcatcaagg ctctgtgccg ggtacacgga aatactgtct ccggaatgga
      901 ttccggcacg ttcgatatgc tccatgatac cagggatcag gatgtcttcg ccgtcgcata
      961 cggcatcgac ttcgatctct tttccgacca gatatttatc taccaggatc gggtgttcct
     1021 gtgcgatccg gttgatgatg ccgatgaatt catctacatc ttcgtcgttg atcgcaatct
     1081 gcataccctg tccgccaagt acataggacg ggcggacgag taccggatag ccgagttcgt
     1141 ttgctgcttt tttggcttct tctgcggtaa atacggtgtg gcctgacggt cttgggatgc
     1201 cgcatttctc cagaatctca tcgaacagtt cacggtcttc ggctgcatcg acgttctctg
     1261 cggatgttcc gaggatcggc acgcccatct tcatcagggc ttctgtcagt ttgatcgcgg
     1321 tctgtccgcc gaactgcact acggctccgt ccggtttttc gaggtttacg atgctctcta
     1381 cgtcttccgg tgtcagtggc tcgaaataca gtttgtctgc gatgtcgaag tcggtactta
     1441 cggtttccgg gttgttgttg atgatgatgg tctcgtatcc ttcttttgca aatgcccagg
     1501 tacagtgtac ggagcagaag tcaaactcga taccctggcc gatacggatc ggaccggaac
     1561 caaggacgag gacttttttg cggtcgtttg tctcgatggc ttcgttttcg ctgccgaata
     1621 cagaatagta gtacggtgtc tctgcggcaa attctgcggc acaggtatct accattttgt
     1681 aggctgctgt gatgccgttt gcataacgca tgtctttgat ctcttcttcg gtctttccgg
     1741 tcaggcgtgc gatcacgttg tccgggaatt cgatgcgttt tgcttctttt aataattctg
     1801 ccgtcagtgg ctgtgtcttc agggctgttt ccatctctgt taagatgcac agtttgtcaa
     1861 tgaaccattt gtcgatcttg gtgatctcat gaatcttatc ataagaaaag cctcttctga
     1921 gtgcttctgc gatcacccag atacggcggt catcgactac agcaagctgt ccggtcagtt
     1981 cctcatcatc caggtgtgtg aaatcgtagg acatcagaca gtctacgtgc tgctccaggg
     2041 agcggatggc tttcatcagg gctccttcga agttgtcgca gatgctcatg acctcgccgg
     2101 ttgctttcat ctgcgtggtc agtgttctct tggcactgat aaatttatcg aacggcagac
     2161 gcgggatctt gacaacacag tagtccagca tcggctcaaa acttgcatac gttttgcctg
     2221 tgatggcatt tttgatctca tccagtgtgt agcccagtgc gatctttgcg gcaacttttg
     2281 cgatcgggta gccggttgct ttggatgcca gtgcggagga acgggataca cgagggttta
     2341 cttcgatcac gcagtattcg aagctttccg ggttcagggc gtactgtacg tnnnnnnnnn
     2401 ngctcgctga tgatgttcag ggcggaggta cgcagcatct gatattcttt atcgccaagg
     2461 gtctgggaag gtgctacgac gatactgtct ccggtgtgca cgccgaccgg gtcgatgttt
     2521 tccatgttgc agacggtgat gcagttgccg gcactatccc gcatgacttc gtactcgatc
     2581 tctttccatc cggcgatgca gcgttctacc agaacctgtc ctactcgtga cagacgcaga
     2641 ccattttcga ggatttctac gagttcttca tagttatggg cgattccgcc gccgcttccg
     2701 ccgagggtgt atgccggacg cagtactacc gggtatccga tgatcttggt aaattctata
     2761 ccgtcttcta cgttttctac gacacgggat gctgcgatcg gttcaccgat cttttccatg
     2821 gtctgtttga attccaggcg gtcttctgct tttttgatgg tctgggaagt ggttccgatc
     2881 agacgtacgt tattctcacg taagaatccg gcttcttcga gctccatggc aaggttcagt
     2941 cctgcctgtc cgccaagggt cggcagtacg ctgtccggtt tttctttcag gatcagctgc
     3001 tctacgactt ctacggtcag aggctcgatg tatacacggt ctgcgatatc tttgtcggtc
     3061 atgatcgttg ccgggtttga gttcagaagt acgacttcga tgccttcttc tttcagggaa
     3121 cggcacgcct gtgttcctgc atagtcaaat tctgctgcct gtccgatgac aataggaccg
     3181 gagccgatca caagtacttt tttaatatct ggattctttg gcattattct gacacctcca
     3241 tcattttcat aaagcggtca aacaggtaac cggaatctaa tggtcccggg catgcttccg
     3301 ggtgaaactg taccgtgaaa atatttttcc cggtgtagga cagtccttcg tttgtaccgt
     3361 cgtttacatt gacaaatgcc ggtactgcta cggacggatc cagtttatcg gtatctacca
     3421 catagccatg gttctgggag gaaatgtata cacggccggt ctgcaaatct ttgaccgggt
     3481 ggttgccgcc gcggtgtccg tatttcattt tgtgcgtatc cgcaccggtt gccagtgcca
     3541 tcagctgatg tccgaggcag attgcaaaga tcgggatatc ggtattgtac agtttgcgga
     3601 tctcttcaat aatggaagta cattctttcg gatctccagg tccgttggaa agcatgatgc
     3661 cgtccggatt ggatgctatg atctcttctg ctgtggtcag tgccggatat acggtcactt
     3721 cgcagccgcg tctgttcaga ttgcgggcga tgtttctctt tgcaccgaag tcaagaagtg
     3781 ctactttcgg tccgtttcct tccaggacgt atttctctgt gcaggtcact ttttctacca
     3841 ctttgcctgt attatatgct gcgatcttcg gaagaacctc acttaaatct tcatattcgc
     3901 aggtggtgat catgccattc atggttccct tttctctcag gatcttggtc agtgctcttg
     3961 tatcgatccc ggcaatacca ggaatgtcgt gtttttccag aaattcctga atcgttcctt
     4021 cacagcggaa attgctcggc attctggaaa gttcgcgaac aatgtatccg tccggccatg
     4081 gctttgcgga ttccatatct tcgtaacaga tgccatagtt tccaatcagc ggataagtca
     4141 tcacaacggc ctgtcctgca taagatggat cagtcagcac ctccagatat ccggtcatgg
     4201 atgtgttaaa tacgatctcg ctgatgattt cttttgtcga tccgatactt tttccagtga
     4261 aaacagtacc gtcctccaga atcaaatatg ctttcattcg tcttcccctt tcgcgtttaa
     4321 tccaatggtt attgtatctt tgtctgtacc ccggactcta aaaaagggta cgtaatagta
     4381 agtccctact acgcccccct cagccctgga tacataaatg ctcaaattgt taagattcta
     4441 ccacaagata atcaagattg caagtacttg tttgcgaatt tttatgcatt tttatgatta
     4501 gatatacgaa taatgtcagg ttcaaaagcc tgaaagcacg atgtgtcggc acaggagttg
     4561 ttttctggca tcttttctgt tcttctgcat atactttttc aagggaattt ttgaggtgtt
     4621 ctatggctac cggaaaatta cagatacagg ttacttccca gctgcgtgct gctcccgtgg
     4681 aggatgccgt gatcgatatt tcaagcaccg gagaaccgga tcagatcctg gaagaagtca
     4741 gaactgacag tgtcggacag acggatacgt tggagctgga ggctccgccg gaggaataca
     4801 gtttaagtcc cggcgannnn nnnnnnactc ggagtacaat ttccgcatta cggctccggg
     4861 ctatgagccg cttacgatct ccggtgcgga aatcctcgca aatcagctgg ctttgcagga
     4921 tatccggttg aaaccgctcg ccggcccgga agcatatgac aatgtcgtga tcccggctca
     4981 cacgctctat ggcgattatc cgccaaaaat cgcagaggcg gagatcaaac ctctctcgca
     5041 gagcggcgag atcgtcctaa gccgcgtagt gatcccggaa tttatcgtgg tgcatgacgg
     5101 tgccccgacg gacagcactg cgggcgatta ttatgtgcgt taccgcgatt atatcaaaaa
     5161 tgtggcttcc agcgaaatct atgccacctg gccgaaggcc gccatccgtg ccaatgtgct
     5221 ggcgatcatg tcgtttacct taaaccgtgt atacacggaa tggtaccgca ataaaggatt
     5281 tgattttacg atcacgtcct cgacagcatt tgaccacaaa tggatctaca gccgaaatat
     5341 ttttgagagt atctcggagg ttgtggatga gctttttgcc gattatttat cccgcccgaa
     5401 cgtcaaacag ccgatcctga cgcagtactg cgacggcaaa cgggtcagct gtccgaactg
     5461 gatgtcacag tggggttcaa agtctctggc agatcaggga tacacagcca cagaaatcct
     5521 ccgctactac tatggtgaca acatctacat caattcagcg gaggagattt ccggtattcc
     5581 ttcttcctgg cctggatacg atctcgacat cggggcaacc ggaccgaaag tggagcagat
     5641 gcaggaacag ttaaatgcga tttcaaataa ttatcctctg atcccgcgga tcgccgtaga
     5701 tggtatttac ggtgaagata cacagaaggc ggtggaagtt tttcaggata ttttcaatct
     5761 tccggtgacc ggtattgtgg actatcgcac ctggtataaa atccaggaga tctatgtcgg
     5821 cgtgacccgc atcgcagagc ttcgttaaat atctaaaaaa gcaggtcaga ctgtgcattc
     5881 tcatcctgat gagtgcacat ctgaccttgc tttataatct actgatccgc cggaagataa
     5941 ccgaaatggc gaagtgcaaa gctcacgcct tcttcttcga tcggcctggt cacgtaatct
     6001 gccacttctt tgagcttatc cacagcattt cccatggcga tgccgtttcc tgccttctct
     6061 atcatttcgt aatcgttcat gctgtctcca aatgccacga tgtcagacca gtctttctgg
     6121 taatagtcca gaagtgcctg gatgccgttt gccttggaaa cttcttttgt gatgacatct
     6181 gcccctctgc ggtcggcaaa aagcggcgtc ttgatttccg gaaatgcttc ccggagcaga
     6241 tcggtacctt ttggatcgcc aaagtatgcc agtccgtaaa acgggtgtgt cagaaggttg
     6301 gactcgtcta tgtactcgcg gtctccgtct ccgaaaatcc gcttttcatg ctcgatcagc
     6361 acttctttgt tgacatagca gccgtatttg ccgatgttca tgccgatgca gagatgatgc
     6421 tcacggcaga actccatcat gcgggcgatc agcttcggat ccatgaagac ttcccggatc
     6481 tttttttcgc ctacggttac tttttgtccg ttgctgtgga cgatcgcatc cggattcagc
     6541 tgctttttat aagctttgct gaactcgctg tccatatcgc gaccggttgc gacagctacc
     6601 acatgacctt cttttttcag tgcttccaga tccttaagca ctttttcgga aatgtgaaaa
     6661 gtcttgtgat ccagaagtgt catatctaaa tcaaaacaaa atattttaga gcacattttt
     6721 taagatttct tcctgtcttt ctttgactga agtctcgcgg atcttcttac gcagcggcag
     6781 aacacatgcc ggtgatacag acagttcgtc gatgcccatt gccaggaatg cttctgtcat
     6841 ctccatatct gccccgagtt ctccgcagat accaatccag atgccgttct tatgtgcatt
     6901 gtctgctgcc attttgatca gacgcagcag acctttgtga tgcggataat agaatccctc
     6961 caggtttgca ttctgtctgt ctacagcggt tgtatactgt gtcaggtcgt ttgttccgac
     7021 gctgaagaaa tcgacttctt ttgccagatc atcgctgatc atggctgctg ccggtgtctc
     7081 gatcatgata ccaagctgga tctctttgtt gtacgggatg ccacttgcat ctaattctgc
     7141 cataacactc tttacgatct ctttgatctg cagtacttct tccagtgcca cgatcatcgg
     7201 gaacatgatg gcaatgtttc caaatgcaga tgcacgcagc agagcacgca gctgtgtacg
     7261 gaaaatatcc tgctgtttca ggcagatacg gatcgcacgg taacccattg ccggattttc
     7321 ttcgtgaggc aggttgaagt aatcaatctg cttgtctgct ccgatatcca gtgtacggat
     7381 gatgacctgt ttctgtccca tcgtctcagc cacatattta tatactttaa actgttcttc
     7441 ttccgtcggg aaattgtcat tttccagata cagaaactcg cttcggaaca gtccgatgcc
     7501 tgctgcatca tttttgatca cagctccgat atcagatgca tttccgatat tggcatacag
     7561 ttttactttc tgtccgtcca atgatacggt ctctttgccg cggaactgct gcagaagctc
     7621 ctgctgtgcc agatactcct gtttctttgc ctgatattct ttcatggtct cttcatccgg
     7681 ctcgataatc aggcatccgt ttgctccatc cacgatggct tcttttcctt cgtatttctc
     7741 atccagtgca tcaccaatac cgatgattgc cgggatgttc atcgtctttg caagaattgc
     7801 ggtatgggag ttcgtagaac cgccctgtgt cacaaaagac aataccatgt ccttattaag
     7861 ctgtacagtc tcgcttggtg ccagatcgtc tgatgcaaga atgaccggct tgtccgttac
     7921 cagtccgctc tcacctgctc cggtaaggat tttcactaca cgggctgaaa tatcttttac
     7981 gtcagctgca cgctctctca tatagtcatc atccatggaa gcaaacatct gtgagaaatt
     8041 gtctccggta acgctgactg catattctgc atttacagac tgtgtctgga tcatgttttc
     8101 gattgcttct acatagtcca gatcttccag catgaactga tgtgtctgga aaagcatggc
     8161 attgctctcg ccaacttcag aaacggcttt gtcatacagt tcaccgagtt cttcaatggc
     8221 ttttgctttt gcattttcga aacgctggat ctcagcctgc ggatcttcta ctttttttcg
     8281 ctgtactaca ccggaatctc tcttaaagaa agcgatctct ccaattgcga tcccaccaaa
     8341 aacacctttt ccctgtaacg taatcattaa gtttcctcct cacttatttt acttatgtaa
     8401 tgctcagtaa cttcgcaatt acaatttttc ttatgaaaca cctgcaatgg cccggcagca
     8461 agtgcttcgg cctgcaccgg atcattacag acattttttt atttagatgc tgtttctgtc
     8521 tcggtttctg ctgttgtctc tgccagtgta ccgtctgcat tttttacaac ggcatttttc
     8581 tcaaggatct tcactgcttc atcctgcaga atacttgcca tgatcgtgct cttaccatac
     8641 tggctgataa atgcatccac atctgtagat cccatctgtg ctgcgtattc gcttgctttt
     8701 tcctggaatt tttcatcgga aatctcaacg ccttctgtct ctgcgatcgc acgcagtacc
     8761 atttcctggc caaggctctg ttttacggtt tctgtcattt gctttacaaa ttcttcttcc
     8821 gtcatgccta cctgctgttt caggaaatct gcatattcca tgccatactg atctgcgtag
     8881 gatttataat aggaggtaac ctgctccagc tgataatcga tcatttcctg cggataatcg
     8941 tttacggttg cattctggta tgcaagtgct accagatcat tgagttcctg gttgtgctgg
     9001 ttttcttttt tcgtcttctc caggtcttct tttatatagt tgttgtatgc ttctgctgtt
     9061 ttgtattcat tattggaaat ttccgctgca agctcatctg tcagctttgg tgcacgtttt
     9121 acggaattga tcttaacggt aaatacaact tctttgcctg ccaggtcggt ggaactgtaa
     9181 tctttcggga aagtcaggtt cagatctacg gtatctccaa tctttttgcc gatcagtccg
     9241 tcttcaaagc cgtcgatgaa gctgccggag ccgatcgtca ggtcgtagcc ttctgctgtg
     9301 ccgccgtcaa atgcttttcc gtttaatttt ccttcgtagt cgatgtttgc cacatcgccg
     9361 ttttctacag taccttcttt gatctcttcg gatttgtcgt tgttgtttaa gtttgtctca
     9421 acctgctgct cgacttcttc gtctgtgaca tcggtggatg ccagtgttac ttccagtcct
     9481 ttgtagtctc ccagtttgac ataatccagt gctttgtagt ccgggcgttc tgctgcttct
     9541 gttgctgctt cactctcaga tgagctgtct gcagttgtct cagaagacgc atccgatgct
     9601 gcttcggttg tggtgtctgt atcttttgtc gtgtttcctg aacatcctgt cacggtgatc
     9661 gccatgccaa gaagcagggc tgcgattact tttcttttca ttgtagttgt ttctcctttc
     9721 agatcttgcg tcgctttaca tttccggtac cggtgtcggt cttgatttat caaagacttc
     9781 tccggcatca aaaagatctt ccagcatttc cggattataa tacatacggt agccgtccag
     9841 atttcttctt ccctgacgca tataattttc tccaaactgt acccagtact gcgtggaatc
     9901 tacattacag taaatatcaa atcccatgct cttaagataa gcgaattttt cgttatctga
     9961 tgtgtatggc tgccagtcgc caaggtcgga accaaaggca aaaatgatca cattggtgtc
    10021 tccgacaagc ggttttacat agtccagcca tttctgtgta tcggtctgca gtctttcaag
    10081 acttgcttcg ccaacattga tatgtcccca tgtatgactt gcaaactccc atccttcttc
    10141 tttcatggca tctgccactt ttttcgcctg ctgtctttcc tttttccagg ctgctttgtc
    10201 gaaatccggg ttctcttcga agaatttctc ctggtactca tccaggtctt ttcttgtctt
    10261 gtatgcgata tcggtgcgat aaccgagtat accgttgtat ccggtcagag caatgattcc
    10321 cttatgtccg tgataggaga aatccggatg ctcttccaca aagcgatcga tcagaggcac
    10381 catatcgtaa tcgcctactg atatacttcc gtcatcttct ttatattcat ttttaatatc
    10441 gccattttca tccacgatca gcttcgttgc catgccatca ccatccatat aatgatagta
    10501 actgacatcg tcctgtgaaa ggacaaatgc ctttttgccc ggcggaagca tgatcttgcc
    10561 cggtgatacg gtaccatctt cattgaccgt acacatatca tgcagactta ccatcacata
    10621 tcctttgtca tacatagact gtgtgatctt gttgaactca tcgatggtag tcataacctg
    10681 attatatccg gcttctttgg aatcaccatc aaatgctttc gaggtgtcat atattaatgt
    10741 atgataaaat acatgcgtca cctggtcaag tggatattct acaagctccg attttgcctg
    10801 ctcgtactct gccactttat cctgcatatc ctgactgtct gcgaaatcag aagatgcctt
    10861 taaaagctca attgcaccgt cataatcata ctgtgctgcc tttaactctg cctgagacat
    10921 gatatcgtct tcttttttct gtgcctgtgt ttctgtctca gctgcactct ctgtgcctgc
    10981 ggtatctgcc gtactctcac tttcagcact ggtcgctgcc tgctcccggt tgtcccggag
    11041 ctttttcttt tcaagcatat cttttccaag aaatgcacac aggccaagga tgatgaacag
    11101 aagaacgact atgatcatcc tccctataaa catctgtcgc ctttttcttt ttttgcgggc
    11161 atccgctcgc ttcttttttt cactttcgtt catattcccc tccacactgg tgataaattc
    11221 gcccttcgga ctactttctc atgtttttac gggctccata gacatactgt cctgcccaca
    11281 tattatgatg ctacggattg ctccgcattt catgctcccg caatcctgaa aacattcgaa
    11341 attcgccctt ctggctgctt tctcatgttt ttacgggttc caaagtcatg cgttttcatg
    11401 taaacatgaa acgcatgact ttttaaccct tgcatcataa tatcacatct ataatagaaa
    11461 ttaccaccct aaattgatga tattcataaa attgtaatta ttcggtaccg cttcttgcac
    11521 tttctgcaaa taaatggtac aatattgaaa tatatgaact tatttaggag ggtgtactat
    11581 gtctacattt tggaatgtcc tcattgtaat tctgctcgtg ctggtaatcg cacttgcact
    11641 tctttattac tttggacaga aagcacagaa aaaacaggcc gcacaacagg aacagctcga
    11701 agcgactcgt caggtcgtct ctatgcttat tattgataag aaacgcctca aactaaagga
    11761 atccggtctt cctcagatcg tcatcgatca gacaccgtgg tatatgcgtc gtacgaagat
    11821 cccggttgtc aaagcaaaga tcggtcctaa gatccagacg ctgatcagcg aaacaactat
    11881 ttttgattct attccggtaa agaaagagat caaggctgtt gtcagcggta tctacataac
    11941 tgagatcaag agtgtccgcg gcggacgagt tgaagcaccg cagcagcaga aaaagagcct
    12001 gcgtgtccgt ctttccgaaa agcttcttcg caagtcaaaa gagctcaatg catctgctgc
    12061 cgaagattcc aagggaaaga aaaagaagaa ataaaaactt tcaagtgcat gcgtatcgag
    12121 agatatggat gcacttttta tttggaattc gcacaaatgc tgatactgca gtctgcaata
    12181 tactcactgt tgatctccag tgcgtatttt acctgcacat caatgctgtc ttctttttcg
    12241 ctgacatcca gatgtgttgc ggagatcccg accaggaatc ttccaaatgg ggtgtcgtaa
    12301 taagattcgc tttttttgtc tttttcgaac accatatgca cgtttgtcat cccgcgtctg
    12361 gtcagttcca gactgccgtt ttcgattttc agaagattgc ggatcacttc attggaatct
    12421 tcctgtactt catcgtactt gatataatgg cggccgtctt tttcaaagta gctgccggaa
    12481 gtgatcactt ccatctcttc actgttttct tctccatccg gaaattgtac accgctgatg
    12541 cggagcatta tatcttttgt catttagtac tcctctatgc tgatcttctt tgtggtattt
    12601 acatcaaatg gcagaatcga atctgcaaat ctcataaatt tatctgcctg gtcgctgaca
    12661 taaaactgat atttacgtgc ttcttcctga gactggatgt ttgtcagtcc tttttctgca
    12721 agaagctgtt tcagctcacg tgcagtctca taagccggat tgaccagtgt caccttttct
    12781 ccgacaattt cacgcagtgt ggaacgcaga agcggatagt gggtgcatcc aaggatcagg
    12841 gtatcgatgt cctgctccat cagtttccga agatatctgc ccgcaacttc aaatgtcacc
    12901 gagtctttca gccatccttc ttctacgagc ggcacaaaca gcggacatgc cttgccaatc
    12961 acctggatct gcgggtcctg tctggtgata taagaagaat aaattcccga atttattgtt
    13021 gcttctgtgg cgatgacacc gatctttttg ttgtgggttg cctcgcatgc tactttcgct
    13081 cccggcttta ctacaccaat gatcggaata tccagctctt tttccacatc ttccagtgca
    13141 taggcacttg ccgtattgca ggcgatcacg atcgctttga catcctgtgt ttttaaaaag
    13201 cggatgatct gtctggaata acgcaacacc gtatcttttg attttgttcc ataaggaaca
    13261 cgggctgtgt ccccgaaata tacgatattc tcttctggca ggtttcgcat gatctcacga
    13321 gccaccgtaa gaccgccaac gccggaatca aaaactccta ccggagcatt acatttgtca
    13381 ctcataaatt attttcctcc gaattaatcc cttatatttt acgtcgaacc cctgtaaatt
    13441 gcaagcagtc ctttccctgc ttagaaaaag accggtgttt tcccgtcaaa tgcataggca
    13501 accggttctt cttttgcaaa gaccgtcttg ccgttcgtcc cttctgtgat ctcttctttg
    13561 acagagacaa gctcttcctg cggaaccagc gtctcgacgg tcactttgtc cgtatactga
    13621 gaatctgtga tcagaagccc tctctgtccg agcagatact ggatcttacc gatcccggaa
    13681 taatcggttt ccatcgccag cagaaagcct tttttctttt cgagtacggt gctggccgca
    13741 agccctgcct gtacagattt tgaatacgca cggacaagcc cgcctgttcc aagcagtgtt
    13801 ccgccgaaat aacgtgtcac cacaacggct gtgttgtgga tgccctctcc gagcagcacg
    13861 tccagcatcg gatgacctgc tgttcctgcc ggttccccgt cgtcactgct tctctgtatt
    13921 tcattatgtt ccccgatcac aaatgcagaa cagttatgtc tggcatccca gtatttcttt
    13981 ttcatctctg caataaatgc agatgcttcc tcctccgagt ccaccggctt tacggtcgcg
    14041 ataaaacgtg attttttctc aacgatctcg ccctctccgc cgcaatagac aattttatat
    14101 tctttcatat gccaaaaccc tccgattaaa attccagaat tcggtaatac ttatgctatc
    14161 ttaccagagt cccccaaaag aagcaacagc agaaatcttc ctttgctctt tctttcctca
    14221 aagttgtgtg ataaaatact aagatgctag ggtcaaaagc ctgaaaccac gatgtgcttg
    14281 cacaggagtg gttttatggc ttaatgcccc gccccaatat ggagcagaac atccagtgga
    14341 tgttcgtttt gctccgaccg aagcggagcg t
//
LOCUS       DAWDWH010000028        14290 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_2370_length_14290_cov_14.329118, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000028 DAWDWH010000000
VERSION     DAWDWH010000028.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 14290)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14290)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14290
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2370_length_14290_cov_14.329118"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3232 a         3699 c         2863 g         4496 t
ORIGIN      
        1 gtttttccgt ttcagtcggc ttttcggtct ggttcggttt ttctgtctca gtcggttttt
       61 ccgtctcagt cggtttttct gtttcagtcg gcttgtcttc tcctgttttt tccggaacgg
      121 tcagtctgat ctgtgccgca ctgctgaaac attttcctgc ctgttcggaa gttgcatcct
      181 ttacgacaaa tttcacatat tttgcggtaa ctggtttgtc aaaagaagcc agtttccagg
      241 aagtgtctgc cgtccagttt ccttctgccg cttttgtata attctcgccg tccatactgg
      301 tgtagatctc gtaagctttg atgataacga agtccgccaa gtttgactgc ctcttccatt
      361 gtaaaaatca gataatgttt ctcgtaggaa gccatgtcgg ctgcatttgt gtagttggta
      421 tgccagattg tatcggaact tccatccagg acattggctg ctgcaccgtt ctcctggctt
      481 gtctcttccg aacctgctgt tacagtcact tttcctgtgt aatcgtttgc accatttgct
      541 gccggatctt tggaactgta atttttcagg atatcttccc cgagttcttt taagtttttg
      601 ccggatgcct ctgtcgtgta ttctttgtga cttcttgcaa atgcccactc aaaagcaaac
      661 cagttgatgt tctgtgtgct ttcgcctttc atttctgcct ttttctgatt gatccacatc
      721 gtccaacgct gtttgtagaa gtcctgtgta agacctgccc actgacggtt ggagtagtct
      781 ttcagtccac cactctctgc ctgcggataa gaagcccagg tagttaccag cgatttcgca
      841 ttcagttcgt agatatcttt tgtgaagtca tcgtcaccct ctgccagttt cttcgcctgt
      901 tctgtccagg ttccgaacag gaattctttt ctggttccga gcacttcttc cactttgtcg
      961 atcaggctta aaaactgatc ggatgcctcg ttaaatttcg caatatcagc ggaccgataa
     1021 gcacttacca tctctctgtg atatttctgg gaagagtttg acagtacctg tttgagaaca
     1081 tctgccagat catagagata accgtcgctc tttttcaggg tatcgtaatc ttcaagcagc
     1141 agctctgctg ctttttccag ctcttccata tcgtagccga tcactgcatt tccccaggtt
     1201 gatgccgcac cgatactctc tgccgggcgt gcattgatgt aggactccgg tgcaccctga
     1261 ccacgcatgt tcagagatgc cttgtaaacg gtgttttcga ggatcttcat tgcctcatac
     1321 gcagattcac tcttggctcc gtaacgtctt gtcacataat ctttgagcca ctggtcggtg
     1381 tcgatctctg tcagcgtttt tgttgcatcg tcacaccata cagtttcaaa taataaatca
     1441 taaaggaccg ggttgttctg agaaccttcc ggtgtaatac cgataccggt cagcatttca
     1501 ctgttgtttg ccgcatcgac aacgccgctg acaatattgt ccatatgtcc atgcagtccc
     1561 atacgtccgc cgaagttgtt cagcatacag taaacccacg gtgtcttctg aaactctttt
     1621 ccgccggagt aactactgtc gttccagtgt gtgtccttct ctgcataaag atccagcaca
     1681 agtgcgtgct cttttcttcc gttcagtccg tttatcagtc cggcactcgg gttgccctgc
     1741 catgcctgga tcacccagac tgcatccttg tcaaactcca gcatggaatt taacacttct
     1801 gaacttacat cacttgtgga catgtcaccg gtatctccgc cctcatggaa tggatccgtc
     1861 gcataataat gtgttacatc tccatagaca tttttctgac actcataaaa taattttgca
     1921 tatttatcgt atgccgctgt ggtggtacgc agcatgtacg gacgctggaa ggagcaccag
     1981 gttccctgtg ggatcacatc gttgcctgtc agtgcatacg ctccttttgc cttgctctgc
     2041 acatcaaccg gaatcatgcc gctgtatccc tgaaggatcg gctgcatgcc gagtttccgc
     2101 atgatcagct ggttctttct tgcaagctcc gttctcttgg taaaccagga gtcatggacc
     2161 ggtccgccgt atccggaaag gtttgccata tatgcccatg catagtaggc aggtcctgcg
     2221 atataatctt tgatctcttc atgcgtatag ccaagtgcac ttaaaaattc tctccagact
     2281 tcttcctgtc cggtgatatc cagaacgatg ttgacaccat tgagtgccag ccagtcaagt
     2341 tcttttctcc actcatcctc gccccagaat gccatcgagt aggacatcgt acagtagttg
     2401 tacgcataac gcagtgccac tttgcactct ttatgtactt ttgatccgac ttttacaata
     2461 ctttttggca tcgttacctg atcaccgacc tgggtgatgc tcaccttgca gtaatatttc
     2521 agataatagt tcaggccgtt tgcgatcgtc acaccgctgt tgccggtgat cttcacttta
     2581 ccgttcacat cttcgatctc ataataatcg gctgcacctt ctgccttgtc ttttacttca
     2641 aaggtaaacc agtttttgta ctgcttacca aggttacggc tgacaatacc ataaacttca
     2701 tcgatcgtat cctgcgtggt aacttctgta ttgtatgcgg attctacata ggaaaccggt
     2761 gcctgaaatg cggctttttt tgcttccccg ctctttgttc cgaggatacg aacttcattc
     2821 agcacggctt tggaacttgc agaattgtag gaaagcagga tacgcacact gcttgctttc
     2881 tttccttctg cctgataaac ctctcctcca tccggacagg catcggtgcc ggttttttcc
     2941 gcaagtttgc tgtaattctg accatcgttg ctgtagtaca gagaatactg ggaatagccg
     3001 tttttcgggg tgtaaacttc aatccttgac agatcatact ctgctccgag atcaaaatcc
     3061 acatatgccg gatactggtc tgcactccag gtattgctta aatttccgtc aacaatgcgg
     3121 ttcagggtgt cctgactgta gttgctgtgc agtgcggtgc ttttccatac gatattttca
     3181 gtatccgcct ctgcgatcgt tgctttgttt gcaaagatct ggaactctgc cagagacagg
     3241 atgttatttt ttccggcatc gtttgctgcc tcaaaaacag cctttacgga ttcgtattct
     3301 agatctttga cagcagaatc cacatcggct gcgttgatct ttactttgtt tgcatcaccg
     3361 gtattgatgt ttccggtcgc aaacggctgt gtttcataag agccgttttt tgttgctttt
     3421 ccgtaaagct tataggtata ggatgcattt tgattgtcct ttggcagtgc cagaataatc
     3481 tgacgcatct tctgctctac gccaaaacca acttctgcaa aggtctcact gctgccggct
     3541 gcaaaggttt tctcctgaag aggagatgcc gttgtctcat cgttgtcggt gatttttgct
     3601 ttgtctgcgg atgcattgac ttcgcttttc agtgtcagac tctgctttct ggtagcagcg
     3661 atgttttcca gttcaactgt ttcttccgca ccgttatcaa tataaacttc tgcctctgcg
     3721 atcgccggcc agaaagcacc ggttgcaccg ttgttcttcg gattggaaag ttccacatac
     3781 agatgagtca tcgcctgggc attttcaaat tcaaacgtat agccatcgtc aaattttgca
     3841 gtctttgcag atccaggtac actgaccaga tcgctcgtta cgttgttcag tgcatacttc
     3901 agtgatacat ccacgctcca cggtgtatgg ccggattcga atttcagcac tatttttttc
     3961 acacatttcg tgtttgcctt tggcagtgca aatgttactg tacacggcca gttcgcccca
     4021 ttgttcaccc acaaactaga cggatttcca tccaccatat tttccttgac gttggctcct
     4081 tcctcgcttg gaacgctgat ttcacagtcg gctgcgatat tggaaagacc actctctgcc
     4141 tgtgtcttca ctttctgctc ctgcacctgt gcattctgga ctggtgcagc cattgcccca
     4201 atgcttccta ccggaactgc cgtgaacatc attacagaag caagcagaac acttaaaact
     4261 ttttttcctt tcataaagtc cctccatctt ttgttttatt ttcagttacc ctcttttatt
     4321 ttccaaacag tccctggaag aaatttacga tcgcatcaat gatccgtgta aagaaattcc
     4381 caacgctctc tttatccaca tggaaatcca aatcttccag tttctgatac aaatcttttg
     4441 cctgatcctt cagtttatcc acatccagat ccagtccctc tatctttttc atcagttctg
     4501 cgatctgctt ctgattttct tcggaaagct gaatatccag ctgatcgcat gcttgatcca
     4561 ccacattttg aatatcctct gcactggtca gtttgttctc tgccacttcc tgttttacca
     4621 gtgccatcag atcttctgct ttttcagaat ctccgatatc ctccaccagt ttgccggtca
     4681 gcaccagttc atcgtttgcc gcatcgagat tctcatcgtc aatctcttcc ccggtcatgg
     4741 tctcatacgc cttgatcgca ccgaccagtg ccgccgttcc ggaaatcgaa aacggtcctg
     4801 ccaccgtgat atccgcatct tcgatgccgg ccgtcaaaag tgcattgcga tacatacctt
     4861 cggtacagta ggaaatattt ttcgttgtta catggatgcc gtttccggct tcttttccaa
     4921 cgaccagaac cgatgacaac gccctggtgc cgatgatcga agcatccaga tacttatcca
     4981 gatattcatg ttcatccgca ttggtgatgc tgatgatatc ataatcatcc acgttgatgt
     5041 cactaagtcc gagcaatgat aaaactgtca tctgttcctg tgggcttaaa tccgctccca
     5101 gtgcaagata tggcttgtcg atgactgcat ccacgcggtc tgcaaagacc gtgacaccgg
     5161 aagtaagcag cattgtcgct gccagaagta ctgctgtaag tttttttctc atatattctc
     5221 ccctttctgt ctgattcaag tcgaacgcac gcgagaatta ctccgttaca aagtaattct
     5281 taccattcgt caagcggata ttcgcaatct gctttactgc ttgatttagt aaaaaagcga
     5341 acgccgtaaa acgtccgctc tcttattcta aaattcggca aactcatctg caagtgccgc
     5401 aaattctttt aaggtcaatg tctccccacg tacatttgcc ggaaaaccaa gcttttcaaa
     5461 agctgtctgg atctgttctt tcgttgctgc aatctccggt gaattattca ggccgttgag
     5521 cagcgttttt cttctctggt taaaagaagc tcggatgatc cggaacatca gactttcatc
     5581 ctttaccgtg gtaggcggat tttcatgcag ggtcagtcgg atgaccgcac ttccgacatt
     5641 cggacgcggc ataaagcagt ttggcggtac atttgccacg atatatggct ctgcataaaa
     5701 ctgcacagcc agagacagtg ccccgtaatc tttggtgccc ggtcctgcct gcatacgcag
     5761 ggcaacttct ttctgaacca taacagtaat gctctttacc ggcacatggc tctcgaaaag
     5821 tcccatgatg atcggtgtcg taatataata cggaagattt gccactactt tgattggtct
     5881 gccctgattg tattcatccg caagtttcgc aatgtccagt ttcaggacat cttcattgat
     5941 cacggttaca ttttcgtaat ctgccagcgt ctcagagagg atcggaatca agttgcggtc
     6001 gatctccact gccacaactt ttccggctgc ctctgccaga tactgcgtca tcgttccgat
     6061 accaggtccg atttccagta cacagtcatc ctttgtgata tctgctgaag caatgatctt
     6121 gttcagcaca tgggcatcga tcagaaaatt ctgcccgaat ttcttctgaa aggcaaaatc
     6181 atatttttta attacttcta tggttttctg tggatttgat aatttctcca ttttttcctg
     6241 cctttcagtc tgtgatgcgg tacatatttc ttgcattttc gtaagtaacc tgctctgcct
     6301 cttctctgga aatgcccttt aactctgcga tcatgtcgag cacataggaa atataggcag
     6361 aacagttgcg ttttccgcga aacggcaccg gtgccagata tgggcagtct gtttccgtca
     6421 gaatccgctc aagtggaata ctctccacca cttcttttaa aactcttgca tttttaaacg
     6481 tgacaacacc gccgaccccg atgtaatagc ccatcttgat atactctttt gccatctctt
     6541 tcgagccgga aaaacaatgt atcacacctc cggtcgttcc ggcgtgctca cttttgatca
     6601 aatcaaacgt atcctgtgcg gcctcgcggc tgtgcacaat gaccggaaga tctacctctt
     6661 ttgcaagctc catctgcttt ttgaaccatt ccttctgaag ctcgtgggac tctttatccc
     6721 agtaataatc aagaccgatc tcgccgactg cgactgtttt ttccatggtg cacatttctt
     6781 tgagatgctg catcttcacc tcatccagtt caccgacatg atccggatgc acgccgactg
     6841 ccgcatagac atgcggataa cgcatagaaa gctccacact gtcctttgcc ccccgaaaac
     6901 ttgcacctac gtttacaata cagccgacac cctgctcttc cagcgtggag agcaggcggt
     6961 ctctgtcttt gtcaaatgcc tcatcatcat aatgagcgtg actgtcaaaa atcatacatt
     7021 cttttcctct tagcagatct ctgctccgga cggcattttc ttttccggta ccatcaggga
     7081 aagttctccg ttctcatcct ctgcacacag cagcatacct tccgaaagaa ctccggcaag
     7141 ttttgcaggt tttaagttta ccagtaccat gacttttttg ccgaccatct cttccggtgt
     7201 gtaatgtgcc ttaattccgg atacgatctg ttttacctgg ctgccgatct taacctgaga
     7261 acagagcagt tttttggatt ttttcactgc ctcgcaggaa atgatctcgc ctacctggaa
     7321 ctgcattttg ccgaattctt caaatgtgat ctcatctttc ggctcgatat caatgccttt
     7381 ttcctcttct tttacttctt cttttaccgg atgaagctct gcaacttttg ccatcacttc
     7441 attgatatcc atacgtgcaa agaggatttc cggtttctcg gttactttat tgcctgacgg
     7501 atacagacca aaggtatcca tctgggaaag cttgcgtttc tgtgctccga gctgtgccag
     7561 tactttatca gaagtctccg gcatgaagga ttccagaagt gttgctccga cgctgatgga
     7621 ctctaccaga ttgtagagaa ccgttgccag acgctctttt tttgcctcgt cttttgccag
     7681 tgcccacggc atcgtctcgt cgatgtattt gttgcagcgt ttgaacagtg taaagatctc
     7741 ggtgatcgca tctgccacac gcagctcttc catttttgca atgactttgt tcggtgtttc
     7801 cagaacgact ttctttaatt cttcatccac tggctcgttt acgttcttat tttctaccac
     7861 gccgccgaaa tatttattgg acatggaaac cgtacggttt accaggtttc cgagggtatt
     7921 tgccaggtca gaattcatac gctctaccat cagctcccag gagatcacac cgtcgttttc
     7981 aaacggcatc tcatgcagaa cgaaataacg cacggcatcc acgccaaaga aatcaaccag
     8041 ctcatcggca tacagtacat ttcctttgga tttgctcatc ttgccgtcgc cctgcagcag
     8101 ccacggatgt ccgaatacct gtttcggaag cggcagatcc agtgccatca gaaagatcgg
     8161 ccagtaaatg gtatggaaac ggatgatatc cttaccgatc agatgcagat ctgccggcca
     8221 gtctttcttg aactgctcgc tgctgtttcc gtcacagtca taaccgatac cggtgatgta
     8281 gttggtcagt gcatccagcc atacataaac gacatgtttc ggatcaaaat ctaccggaat
     8341 gccccattta aaggaagtac gggatacgca cagatcctgc agtcccggaa gaaggaagtt
     8401 gttcatcatc tcattttttc tggaaaccgg ctggataaat tccggatggg tgttgatatg
     8461 ctcgatcaga cggtctgcat atttgctcat acggaagaaa taagcttctt cttttgcagg
     8521 ctgcacttca cgtccgcagt ccggacattt gccgtccacc agctgagact cggtaaaaaa
     8581 agactcgcaa ggtgtacagt acataccttc ataatgtccc ttgtaaatat ctcccttatc
     8641 gtacagtttt ttgaagatct tctgtacctg tttttcgtgg tctgcatccg ttgtacggat
     8701 aaatttatcg taagatgtgt tcatcagatc ccagattctc ttgatttctg tggaaacttc
     8761 atccacaaaa gccttcggtg tcacaccttt ttcttctgct ttcagctcaa tcttctgacc
     8821 gtgttcatcg gttccggtct ggaagaacac atcatatccc tgctgtctct taaaacgggc
     8881 aatgctgtct gccagcacga tctcgtaagt gtttccgata tgcggtttgc cggatgtata
     8941 ggcaatcgca gttgtcatat aaaatttctt tttatccatt tctgcctcct gtatattgtc
     9001 agatcaaacg ccggtgaagg cattttcttt gactaaatta acacaggaaa actgcgtctg
     9061 gcagctttcc tgtacttttt gcttattcag cttttaacgc ttatttctac cgtaacatac
     9121 gacggttttt ctgtcaagat ttcctgactg tgaagttatg gaacggttat acgatttctt
     9181 agaaataacc aaagcttttg ctctttgtat tccagtagta gagtcttccg gatttattga
     9241 ccttggagcc gtttttcttt ttggtttcta catacatata gtaagtcttc ttcgggttga
     9301 tctttttgcc tttaaattta cggatggtca gctttgtggt gttcttgccg accgatttta
     9361 cttttttata accttttttc ggctttgtgg agatatacac atcatatccg cttgctccgc
     9421 cgacttttcc ccactggaag ctcaggctgc cttttttcac cttgatgctt gtctctttga
     9481 tacgtgcctg gttgaaacac tggatctttg ccttcggtgt agcataagtc tttccattta
     9541 ccgtcttgaa agcctgaatg gttaccgtgt aaacttcatc tttcatctta tcgaaagatg
     9601 cactgtaagc atagctgttc agtttctgtg tcttgccgac acgtctgcct ttgctgttat
     9661 acagggtcaa ctttatccca tgttacttcc agcttgttga taaagtacca ccaacgctcc
     9721 tgtctgaaat tatggacggc tgccgggatt gtctcaacct gtccgcttac cggtgaactt
     9781 gtttttgttc catttgatgt gtaagcttct acacgcacat tgtaacgctt accatctttc
     9841 agtccggtca gtgtatagga tggtgtcttg gattttccaa ccagcttcat atcactgtaa
     9901 ctttctccaa gataaatatt atatccttct gttgtaagtg tattcataga gcttgcatac
     9961 gtctgttccc aagtgatact tgcactgttt ttagtcatgg ctgtctgctg aagattcccc
    10021 agtgacacat aataatatgc attctctgct gcctgtacct gtttcgctcc ggttcctgct
    10081 acagttccga cacttcctgc tgccagaatc attgccgtaa gaagcatcat actaagtttt
    10141 tttccttttt ttatcatgtt tttcctcctt tgccttgtgt tgtggttttg ggttctgaac
    10201 tttttctaaa attaactcaa tgagtcatac gtgatttttc cgtgccttgc actctcaaaa
    10261 tcacgaaaac attcgaaatc cgctcatttt ttacaaaaat ggctgctttc tcatgttttt
    10321 gcggtcccca tagacacaaa cacgtatgca agcatccgtt ttgtgtccat ttgtcccttt
    10381 gactcattat atcatttttc tgcttccacc gctactcttt tcggcaatgg caggaaaatt
    10441 ttttccacaa tcaagaagga acacatcatc agcattgcac cgatcacaaa ccgcacaccc
    10501 ggatgttctc caagaatcag gattgagaga atacatccaa atacgctctc aaaagaaagg
    10561 atcacgccgc ttgtggaagt tttcacatat ttctgtccaa acacactgag caaatacggc
    10621 acaaccgtgc agaagactgc cagataagcg atgcttccga tgttcatgcc ggacatggct
    10681 gccggatgtg gctccaggat catcgctgcc acaagtccca aaatgccgcc cgttccaatc
    10741 tgaagcatat ttaacagaaa gccgtcctct tcccttgcaa aaatcccggt cacaacaata
    10801 tggatcgcat aacacactgc cgagagcagc gagattagat ctcctttgtt gaccccgtca
    10861 aaacttccaa gcaaaataaa gccaagtccc acaatacaga caagtgccgc cagaatatcc
    10921 gccctctgcg gcttttttcc gcggatcacc cagtaaataa gcggcaggat cgccacataa
    10981 gatcctataa taaaggaaga tttggaagag gtcgtgtact gcagtcctac cgtaaagaac
    11041 agaaattcaa aatacaggac aatgcccatg atgatgccac gctttaacgt gcggatgctc
    11101 atttttttca ttctcgggat acagaccaga gtcataatga tacccgccag cagaaaacga
    11161 aaggccagaa gccagatcgg agagacattc tccagcgtgt cttttaacac gcaaaaggca
    11221 ctgccccagg tgaatgccgc caggaacagt gcgaaaattg caattgtttt tttcatttat
    11281 ttttcctttg taatactaaa gagatgccca cgaattgctc cgtcacaaag taactctcgc
    11341 aattttcaga tgaactggtt tgttttttca tcgtagtgat aatctatgct gtccattttc
    11401 tccagaaggt tctgccggtt cagtccgcgg caggtacaca gtgcctccag tgattcataa
    11461 taatcacgca gctgtgtgtt tacatagctc aacaatatca tcggatcttt tggaatcata
    11521 ctttactcct ttttgcatac ttatggaatt ccggagcgtc acttccaatc tctgtgccag
    11581 cctgacatct ctgtcccgtt atcacatcat tcgactttca ttacttccgc tgccagtttt
    11641 ccgtctttga gaccgatcac gatggtgtct tctgtctgta catttccaga aaggatcatc
    11701 cttgctgcca gtgtctccac atttttctgg aggaaacgtt tcagaggtct cgcaccatag
    11761 accggatcat aaccaccgtc tatgatatag ctttttgcct catccgtcaa ctccagcctg
    11821 atctcctggt ctgcgagtct gcggttcaga tctgccatca tcagatcaac gatattgccg
    11881 atgttttctt ttgtcagcgg cttgaacatg atgatttcat ccagacggtt taaaaactcc
    11941 ggtctgaaat gtgcccgcag gtcgttcatg accagatcct ggctctcctg acggatgctt
    12001 ccgtccggct caatgccatc cagcagataa gacgaaccaa tgttagaagt catgatcaga
    12061 atcgtattct taaagtctac ggttctgccc tgggaatcgg tgatacgccc gtcatccagt
    12121 acctgaagca gtacgttaaa gacatccgga tgtgctttct cgatctcatc aaacagtaca
    12181 actgagtacg gttttcttct gactgcttct gtcagctgtc caccttcttc atatcctaca
    12241 tatcctggag gtgctcctat cagacgggac acagaatatt tctccatgta ttcactcata
    12301 tcaatacgca ccatattctg ctcatcatca aacatacttg ctgccagagc ttttgcaagc
    12361 tctgtcttgc cgacaccggt aggaccaagg aaaaggaacg aaccgatcgg cttggtcgga
    12421 tctttgatac cagctttgga acggatgatc gcatcggtca cttttctgac accatcttcc
    12481 tgtccgatga cacgtttgtg cagttcatca tccagatgca ggatcttggt acgctcacct
    12541 tcggtcagtt ttgcaaccgg gatgccggtc catctggaaa tgatccttgc gatctcttcg
    12601 tctgttacac ggtcatgtac cagcgacaga tctttgcttt ttaccttttc ttcttcaatt
    12661 tccagctgtt tctgaagttt cggcagttcg ccgtactgca gctctgctgc tttgttcaga
    12721 tcatagcttc tctgtgcttt ttcgatctct ttgttcatat tttcaatctt ttcacgcagc
    12781 acggacagat tttcaacaga atgtttctcg ttatcccact gagctttttt gcctgcaaaa
    12841 gattcacgct gttctgccag ctccttctgc aggttttcca gacgctcggc acttaattta
    12901 tcggtctctt ttttcagggc agcttcctcg atctccatct gcatgatttt tcttcgctgt
    12961 tcatccagct ccgttggcat ggaatccagc tcggtcttga tcagggcaca ggcttcatcg
    13021 accaggtcaa tggctttatc cggcaggaaa cgatcggaaa tataacgatg agaaagcgtt
    13081 gcagccgcta ccagtgcact gtctgtgatt tttacgccat gataaacttc atagcggtct
    13141 ttcagtccac gcagaatgga aatcgtgtct tctacgctcg gctcgtctac cataaccggc
    13201 tggaatcttc tctccagtgc agcatccttt tcaatgtact gacggtactc atccagcgtt
    13261 gtcgcaccga tgcagtgcag ctcacctcgt gccagcattg gctttaacat attgccggca
    13321 tccatcgctc cgtctgtctt tccggctccg actatcaggt gcagctcatc gataaagaga
    13381 atgatctgac cttcgctttt tttgacttct tccagaacgg ctttcagtcg ttcttcaaac
    13441 tcgccgcggt atttggcacc tgctaccagt gctcccatat ccagtgagaa aatcttttta
    13501 tctttcagtc cttccggaac atcaccgcgg acgatacgct gtgccagacc ctctacagct
    13561 gccgttttac caacaccagg ttcaccgatc agaaccgggt tatttttcgt ttttctggaa
    13621 agaatacgga tcacattacg gatctccgca tctcgtccga tcaccggatc cagcttctga
    13681 tttcttgctt tttccaccag atcctgacca tatttattta acgtatcata agttgcctcc
    13741 ggattgtccg tcgtcacacg ctgattgccg cgtaccgtgg aaagtgcctg cagaaaacgt
    13801 tctcttgtaa tgccgaactc ctgaaacagt ttcttgatct ccggatttgg attcttgatc
    13861 atggacagga acaaatgctc tacagaaaca tattcatctc ccatagcctt tgcctcgtcc
    13921 tctgcctgaa caagtacttt atttaaatac tgaccgatat aaacctgtcc gccggaaacc
    13981 ttggtacgtt tctcaagtgc agattccacc gcctcggtaa aatacgtttt atcaatctcc
    14041 atcttctcaa tcatcttgag gatgagactg tcttcctgtt ttaacagggc ataaagcaga
    14101 tgctcctgct cgatctcctg attgccatat tcatatgcca gtttctcaag gctgttcact
    14161 gcctcgatgg acttctgtgt aaatttactg atattcataa aaacacctcc tgtttgccag
    14221 atgttatatg tttctttgtt atattgagaa tataacacca tttgttagca ctgtcaagag
    14281 gtgagtgcta
//
LOCUS       DAWDWH010000029        13463 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_2528_length_13463_cov_15.263201, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000029 DAWDWH010000000
VERSION     DAWDWH010000029.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 13463)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13463)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13463
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2528_length_13463_cov_15.263201"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8201..8210
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9201..9210
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11308..11317
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3598 a         3360 c         2603 g         3872 t
ORIGIN      
        1 gaatcttttt cgagtcgacc tcacccttgg aaagcaagac aaccgtctca acgtgatcac
       61 aaaacggaaa catatcaacc ggccaggcct cctgcatctg atagcctttt ttctttaaaa
      121 tgcgaagatc tcttgccagc gtttccggat tacaggaaac ataaaccacc tttttcggtg
      181 ccagttttgc aaggcaatcc agaaatactt cactgctgcc actgcgcggc ggatccatga
      241 aaacaacatc cagctgctga tcttctgcag ccattttcag cataaattct ccggcatctt
      301 cgcagtagaa agtgatattt tttgtctgat ttcgctttgc atttttaatg gcatcacgga
      361 cagcttcttt attaagttct acgccggtca cctgttttgc ctttgatgct gcgatcagac
      421 cgatcgtgcc gatcccacag tacgcatcga gcacggtttc ttttccggtc agccccgccg
      481 cttcgattgc tttctgatag aggatctccg tctgtaccgg attgacctga tagaacgact
      541 gtgcagaaat gcggaacgtg cagccacaga gtttatcttc gataaatcct tttccataga
      601 gaacgacgtc tcgtgttcca agtaccatgc tggttccccg gtcgttgata ttctgaacga
      661 ccgtcgtaat ctccggatga acttttcgca gtgctttgac aaaattgttc ttggatggaa
      721 ataccggcga tgccgtgacg agcacgacca tgatctctcc ggtagaaaat ccgtgccgga
      781 tcagcacatg acgcaaaagc ccatactgtg tatcttcatc gtacgttttg attttaaaag
      841 atttcagaag tcctcggatc gtgacgatga tctcatctgc tttctgatcc tcgatcatgc
      901 aggagtccac cggaacaacc ttgtgtgtgc ctttctggta aacaccggaa atcgcattgc
      961 ctctgcggtc acggtcaaac acagcatgca ctttattgcg gtaatgatac ggatctttca
     1021 ttccgatgat cggatgtact tttccaaaag aatccagaag gatccgcaca cgtttttctt
     1081 tgtgcctgag ctgttctgca taagacattc cctgaaattc acagccgccg cattttttcg
     1141 ccaccggaca gactgctgct ttttttgttt tgtttttttc catgaatgat tatttgcaga
     1201 gttctgcaac cacctggttg atgacttttc cgtctgcttt tcctttcata tctgccatta
     1261 catttttcat gatcatgcct ttattttttg tagcaacgac ctcggcaaat ttttcctgaa
     1321 tataagcttt aacctcttct gcactcatct gagccggtgc atattctttg attacatcgt
     1381 aacggaactg atattctgct ttaagatcct gccgggagtc cggacaggta tcaatctgtt
     1441 cttttactgt tttcagctct ttcagaatta cctgatcaac catgctttcc ggaatatcat
     1501 ctctgcatcc ggcatcgatc gctgcttttt ttactgcaga aaccagagaa gaaattgctt
     1561 ccttgcgttc tttatctttt gccttcatag ctgctaccat gtcttttctt aatgcttcta
     1621 actgcatttt taaatcctcc catcattcct ggataaaact ttttatccca ttgtcatttt
     1681 tgctcattat agcacttctc tttctttcct gtaaagtttt cactgttttt tttctgattt
     1741 tgtgctataa taattccact gatgacaggg tcaaaagacg atcaggtact ttatctctgg
     1801 cattaccatt ctcaaaccga aaagaaaggt ggaatttata tgatcattgc tattatctct
     1861 atcgccgtcc ttgcggctgt gatcgtttcc tgcgtagttg ctgtggttgc cggtgtaatc
     1921 ggcagcaaat ctcacgatga agacgatgcc tagaacacaa cgaccagtca cattttaaac
     1981 tgctcttttc ataaaacttc tccctctacc gacactgtaa gcacctgcca tggtataaac
     2041 ttaccacttt ttttcattgc tgtcacagct gcattttgaa ttccgccgca gcatggcact
     2101 tccatcctta tgattgtcac acttttcaca tcattctgtt ccaggattgc cgtaagtttc
     2161 tcactgtaat caaccggatc cagtttcgga catccgatca gcgtgatttt tcccttctgg
     2221 atctcttttg caaaatttgc gtatgcaaac gcagtacagt ctgctgcgat taaaagatct
     2281 gccccgtcaa aataaggggc attgaccggc gtaagctgaa tctggatcgg ccactgcctg
     2341 ttcttttttc tcttatttgc ttccacagct gcttcgtcat atgccgctgc ttcccgctca
     2401 acaaacgtga tcgctccggt tgggcaggtc ggcagacagt ctcccagtcc gtcacaataa
     2461 tcatcccgca ataattttgc ctttccattt accataccaa tcgcattttc atgacaggct
     2521 ttcgcacata aaccacatcc attgcatttt tcttcgttta tctcaataat ttttcttatc
     2581 atttatctat attcctccta ataatcacac ctgaaatctt tttttcactg ccgggcagac
     2641 agcaattttt ttcaatcact gtccatacct tcaccgtgcc ccttgatttt ctgttttccg
     2701 tgtagtatga tgctgaacaa agccagcaga ttttctgtca aaccattttt tccggtctaa
     2761 aatctctgcc tgactttgac ctgatcctaa cataacatct gagaaaaatc ggtggttata
     2821 accactgaat cacaatcaaa ataccgttaa atatcaacag tactattttt ccgcatactg
     2881 ttgtttttac ctgacaggag actttatgaa ctttttagaa ctgcctctgt ttcagagcct
     2941 ttccgatcag gaactggaac aaatgaagac ttccggctgt ctgcggacgg cttcttacaa
     3001 aaagaatgat attatatttt ctaccggttc cactgtttct gaaatcggca ttgttttatc
     3061 cggaagcgta aatgtagaaa acattgattt ctggggaaat aagagcattc tgggaaatat
     3121 gaatgaagga catatttttg cagaaaccta tgccctgtgc cgggaaccga tgatggtaga
     3181 tgccgttgcc gccagcaact gcgaaattct ttttttgaat ctgaaagttt tacaggaaaa
     3241 aaactgcact tcttcctggt attcgaaaat acaacgcagc cttcttgcaa tgtgcagccg
     3301 caaaaatttg atgctctcag aacggatttt ctgcacttct tcaaaaacag ttcgcagccg
     3361 actgctgacc tatctgtccg cacagtcccg gaaacacggc aaccatcgtt ttcagattcc
     3421 gttcgaccgg cagcaaatgg cagactacct gaatctggat cgcagtgccc tgtcaaaaga
     3481 actttcccgt atgaaaaaag aagggatctt agattttcac aaaaacgaat ttgttttaaa
     3541 aaatgagatg ctctgaattt ttcacacatc aaaatgtatc atattggggc aaaagtcccc
     3601 aactatattg gaggaattga atatgtgtac cgcagcaact tataaaacaa aagattttta
     3661 tttcggacgt actttagact atgaattttc ctatggtgat gagatcatca ttacaccacg
     3721 aaactatcct ttcgttttcc gccacacaaa aacactggaa aatcactatg cactgattgg
     3781 catggcgtgc tgtgccgaca attatccgct ctattacgat gccatcaacg aaaaaggact
     3841 tggcatggca ggactgaatt ttgtcggaaa tgctcattac cagccgcttt ctgattcaga
     3901 agaaaatatc gcatcttttg aatttattcc atggatttta ggacagtgtg ccaccgtaaa
     3961 agaagtaagg agctttcttg gccacatgaa tttatgtgac acaccgttca gcgaccagtt
     4021 tccatcatcc agccttcact ggctgatcgc tgaccgcagc gaaagcatca ccgtagaatg
     4081 tgttgcagat ggcatgaaaa tttatgacaa tccggtcggt gtactgacca acaatccacc
     4141 gtttgacatg cagctgtttc agctcaacaa ttatatgcag ctttccgcca aatcaccgga
     4201 gaatcatttc tccgataaat taaacctgaa cacttacagc cgcggcatgg gggcactcgg
     4261 acttccggga gacctctcct ctcagtcacg tttcatccgc gtggcattca ccaaaatgca
     4321 cgctatttct gatgaaagcg aagcttccag tgtaagccag tttttccata ttttaggttc
     4381 cgtggaacag agcagaggct gctgcgaagt tgccgaagga aaatatgaaa tcacgatcta
     4441 cacctcctgc tgcaatgccg ataaagggat ttattattac acgacgtatg ataatcatca
     4501 gatcagtgcc gttgatatgc ataaagaaaa tctggaagca aaagaactga ttcgctattc
     4561 cccgataaaa gaagagcaga tatattttca gaacggcaga taagaagttg tcggtggttt
     4621 cctggcgggt gtaaggaatt gaaatgccga gagtgttatt aggatactgt ctaagaaaga
     4681 acaataatgc atgtgcataa aataatacag ccatgatgaa ttcgaaaaca aatcgctctg
     4741 acggtagcaa ggcttccgtc gtgaggtttg cgatgttgcc gccccagaga gccgctgtct
     4801 gagcctcgaa gaggcgagtt taggcgaagg gcggctaaca cacgagcgaa ccgaacagga
     4861 aacgaagcgt gtcaggagat tgttttcgaa tcatcatggc gtggctattt tacagactat
     4921 tttttactac attcattttg cacattgcaa aaagctcagc tcagctcaaa cattccatga
     4981 taaagctgat aatatttccc tttctgttcc aacagttctg catgatcccc tctctcgacg
     5041 atcttcccct gctccagtac catgatcgca ttggcatttc gcacggtact taatcgatgt
     5101 gcgataacaa acacggtcct tccatcagct gatccatgcc tttttcgatc agttcttcgg
     5161 ttcgtgtatc aacagaggat gtcgcctcat caagaatcag aaccggcgga tcggctactg
     5221 ctgcccttgc gatcgccaga agctgccgct gtccctggga aagatttgcc ccatctgccg
     5281 tcaccatggt atcgtaacca tctggcagtc gtctgataaa agagtgtgca tttgcaatgc
     5341 gtgccgccct ctcgatctct tcctgcgttg catccagttt tccaaagcgg atgttatccg
     5401 ctacggttcc ggtaaacaga tgcgtatcct gaagcacgat gccaagagag tgtcgcagtg
     5461 catctttctt tatctttcgc acatcaattc catcgtaaac caccgttcct ccctgcacat
     5521 cataaaagcg gttgatcagg ttggtgatcg tggtttttcc ggctccggtc gatccgacaa
     5581 aagcaatttt ctgtcccggt tttgcataca ggctgatatt cttcagaatc atatgctctt
     5641 tcgtataacc aaaatcaaca ttttcaaaac ggacatcacc gcgaagcatc gtcagttctc
     5701 catcttttga acgccatgcc cattttccag tcttttcctg acattctttc aggatgccgt
     5761 tttcttttct gacattgaca agcgtcacat ccccttcgtc cacttccggc tccagattca
     5821 tcacatcaaa cacccgctct gctccggcaa gtgcggagag caggaaattg ctctgctgtg
     5881 taaactgatt gatcgggagt gccgcctgac gcacaaacac cagataactt gcaagacttc
     5941 cgacatccgc catcccacgc atcgccagaa ttcctccgag aactgccacg attgcataat
     6001 taatataaga tatacttacg actaccggaa ccatcgttgc cgcataagac tgtgcaccgg
     6061 ttccttcttt tcgcagtttt tcatttttct ccagaaattc ctgaagactt gcctgttcat
     6121 gattaaagac tttgactact ttctgaccgc tgaccatttc ttcaatatag ccgtccagct
     6181 caccaaggct ctgctgctgt ttcgcataaa aaaccttact ttttgctccg ctgaatttca
     6241 tataccagaa catgatacca tagcagaccg tcacgatcag cgacagtttc cagttcagga
     6301 tatacaggat cacaagggtt ccgatcatct ggataaaact ctggattgcc atcgcaaaac
     6361 tgttattgag agcatctgcg atcgtatcaa tatcgtttgt gaacagactc atgatatcgc
     6421 cgtgtcgctt ctggtcaaaa aagcgaagcg gcagtgtctg catatgtgca aacaaatctc
     6481 ttcgaatatc aaaaacaacc tgctgggcag cttttaccat cgtctgagaa tagccccagg
     6541 ctgccagtgc tccgatggca aaaatgccgg ctgccacaag cactccgatg gcaagtgaat
     6601 gcagatcccc gtttgcaaga ttattgacaa ccggacgtat catgtaagtt ccaagaaggt
     6661 ttgcaagggc acttaccgtt accagaatac tgacgatcag aagcataaat ttatgccgtc
     6721 caagataaga aacaaaaatc cgcagtgtgt gctgcatatt ttccggccgt ttaacattct
     6781 tcgttttcga tgccatcgct gtttcctcct ttcatctgag aagcataaat ttcctgataa
     6841 attgcatcct ccttaagaag ctgttcatgc gttccgatat tatggatctt accgtcatcc
     6901 aggatcacga tctgatccgt atggatcaca gaagaaattc tctgtgcaat gatgatcttt
     6961 gtcacatcgc caagtttttc cagctcttcg cggatcttct tttccgtcgc cgtatcgact
     7021 gcactggtag aatcatcaaa gatcaggatc ttcgcattgg caagaagtgc tcttgcgata
     7081 cagagtctct gtttctgccc gccggacagg ttatttcctc cctgcacaag catggtgtcc
     7141 agtccgtccg gaaaacgctc caggaactca tccgcacatg ccacacggca tgcatgccag
     7201 attttttcgt catctgcatc cggatcgccc catttcaggt tatcacgtat cgttccggaa
     7261 aaaagctcat ttttctgcag aataattccg actgccgccc gcagtgcatg aagatcatat
     7321 gcttttacat ccacaccgcc aactctcacc gtgccgcagg tcgcatcata aagcctcggg
     7381 atcagctgca cgagcgtcgt tttggcggct ccggttccac cgaccacacc gatcgtctgc
     7441 ccggccttga tgtgcagatc aacatctgac agtgcatacg ctttcgcatc tttgtgatat
     7501 ttaaaagata cctgttcaaa atccacgctt ccatctttga ccttccgtac cccgttttcc
     7561 ggagacttca gaacaatcct ctcatccagc acttctgcga tacgatgtgc actggcaaga
     7621 gatctcgtca acagcagaaa aacatttgcg atcatcatca gagcgttgac aacctgcatg
     7681 acataactta aaaatccggt cagatcacca accttaagac taccgcttaa gatcatattt
     7741 ccgccaaacc acatgatcag tacgatcgca ctgtacatga caacctgaaa tgccggaaga
     7801 ttcagcaccg catagtgaaa agtctgttca cttgaaacac tcagatctgt atttactgca
     7861 tcaaattttt cttcttcgta ctctccgcgg acaaacgcct tgaccgcacg gatcgctgtc
     7921 acaccctcct gcacaacatg gttcagacgg tcaacgattg tctgaagctt tgtatacata
     7981 ggagccactt tccggacgat cagaaataaa acaatgccaa gaaccggcgt acacaccaga
     8041 aaaacaatgg caagtttcgg attcatccag aaagaaagac cgatgccgag gatcagaagc
     8101 gacggacctc tcgtcaccgg acggaagccg gaattgatca gattctgcag aaccgtgaca
     8161 tccgtggtca ttcgcgtgat cagagacgag gtatcaaaat nnnnnnnnnn atttgagaaa
     8221 gaatactgca tcacttttgc atattctgcc tccctgatct tcgctcccca tccataagaa
     8281 gccctcgctg caaaccgtgc atacagaagc cccgtcacca gagccagaag tgcacagatc
     8341 cccatttgaa agcccttgga ataaatgtag gcaagatcgt ggtttgccac cccgacatcg
     8401 atcagatccg cgatcatgat cggaatgaca agctcaaacg ctgtctcgat cattaccatc
     8461 aaagcaccaa gcatcatatc ttttttatat gacccgatat atcctgccag ccgctttata
     8521 tcattcatat tttattttca ctcctttttc agaaaccctg agcaatctca gtctttgatg
     8581 caaagcggac attcacaatc ctctttcgca ctctgtcagt tggtcagttc cttcaaaagc
     8641 tccagatcct ctgcctttat tctcagattc gtctcaatct tttcgccatc cggtgtggta
     8701 accgtgatcg ccatgatgct gccctccttt aaagcccgtt tggaaactgc attgcagaat
     8761 gccgggaatt tcgggtggtt tcttttaaat atattccatg cattcataat cttcatcata
     8821 tttcctatat tattcagtgc catttgtttt acctcctaat attcattttt acatctgcta
     8881 agtttaaagt actttctcaa actgtgcaag acacaattac cacaaattga gccacataat
     8941 cactcccgta caggcaggaa tgacatttag accttttgac cctggcatca ttatataatt
     9001 ttcacatgaa aaaaatattc tatttatatg ttcgcctgac taaacgttca aaactgagca
     9061 actctcgtgc atgatctggc gttttttccc cgcttttttt gctgttattc gttcaaactc
     9121 attcataaac agcagatttc atacattttc agcaaaaagt tcagaaagtt tttattgatt
     9181 ttgccgggta aatatgtata nnnnnnnnnn ataatgatga tagagaagtg ggcatcattt
     9241 ctttaacttt aagtataaaa gaaaagaggt taatcagatg gcaaaaattg atttaagcaa
     9301 gtatggcatc actggaacta ctgagatcgt gtacaatcca tcttatgaga tgttgttcga
     9361 agaagagaca aaacctactc tggaaggctt tgaaaaaggc caggaaagtg aactgggtgc
     9421 tgtcaacgtt atgactggta tctacacagg tcgttctccg aaagacaaat ttatcgtaaa
     9481 agatgagaat tccaaagaca ctgtatggtg gacaacagat gaatataaaa acgacaacca
     9541 tccggctact caggaagcat gggatgctgt aaaagcactg gctatcaaag aactgtctaa
     9601 caagagactg ttcgtagtag atgctttctg cggtgccaac aaagacaccc gtatggcaat
     9661 ccgtttcatc gttgaggttg cttggcaggc tcactttgta acaaacatgt tcatcaaacc
     9721 gactgctgaa gaactggaaa acttcgagcc ggatttcgtt gtatacaacg cttccaaagc
     9781 aaaagttgaa aactacaaag agctgggtct gaactctgaa acagctgtta tgttcaacat
     9841 cacaagcaaa gaacaggtta tcgtaaatac atggtacggc ggagaaatga aaaaaggtat
     9901 gttctctatg atgaactact tcctgccgct gaaaggcatg gcttccatgc actgctccgc
     9961 aaacactgac atgaacggtg aaaacacagc aatcttcttc ggtctgtccg gaacaggtaa
    10021 aacaactctt tctacagacc cgaaacgtct gctgatcggt gatgacgagc acggctggga
    10081 tgacaacggc gtattcaact tcgaaggcgg atgttacgca aaagttatca acctggacaa
    10141 agaatccgaa ccggatatct acaatgcaat caaacgtaac gctctgcttg agaacgttac
    10201 tctggacgaa aacggcaaga tcgacttcgc tgacaagagc gtaacagaaa atactcgtgt
    10261 atcttacccg atcgaccata tccagaacat cgttcgcccg atctcttctg caccggctgc
    10321 gaagaacgta atcttcctgt ccgcagatgc attcggcgta ctgcctccag tatctatcct
    10381 gacagaagct cagacacagt actacttcct gtctggtttc acagctaaac tggctggtac
    10441 agaacgtggt atcacagaac cgactccgac attctcagct tgcttcggcc aggcattcct
    10501 ggagctgcat cctacaaaat atgcagaaga actggttaag aaaatggaga agagcggtgc
    10561 aaaagcatac ctggtaaata caggatggaa cggaacaggc aaacgtatct ccatcaaaga
    10621 tactcgtggt atcatcgatg ctatcctgaa cggagatatc aagaatgctc ctacaaagac
    10681 cattccatac ttcaacttcg aagttcctac agaactgcca ggcgttgatc cagctatcct
    10741 ggacccacgt gacacatacg ctgatgcttc cgaatgggaa acaaaagcaa aagatctggc
    10801 tgacagattc atcaagaact ttgctaaata cgaaggaaat gctgccggta aagctctggt
    10861 ttccgctggt cctcagaaat aagaataaga ctgccgaaat caaacttttc aaaagcttat
    10921 acaaaaggac accgcaaaat tgcggtgtcc tttttttatg ccgaaatgcc tccgcatggc
    10981 agtccagatg gagcacattg cgcccattgc cctgcggcgg ggaatttgtc gtgagcagat
    11041 acattttaca tttatcatac ctgaaaataa gactattata aacacaaaac agtcgccgtc
    11101 acacgttttt tgacgctgct gcttcatacg cctgctgata gaaggtttcc tgagcatcca
    11161 ccggacgctc gatcgtcatc gggattctgt aatttccttc tgaatcctca tggcgggcct
    11221 tctgattatc atttaacatg atccggaaca tctcaagcag acgttctctg attgccggat
    11281 cgcagacagg agctgcgact tccacacnnn nnnnnnncgt catataatcg gctgacgcaa
    11341 tataaacttt ctctcttccc ttacttccga aaatataaat tctcgaatgt tccaggaatc
    11401 ttcctacaat gctgatcaca cgaatatttt ccgttgcatc ttttacgccc gggatcagac
    11461 agcagatgcc gcggatgatc agatcaatct ttactccggc acaggaagct tcgatcagcc
    11521 tgtctatgat ctttttgtca gtaagtgcgt tcattttcag cccgatataa gcatcttttc
    11581 cggcaagtgc agctgcaatc tcatcatcga tcatcgccag cactttattt tgcagacaga
    11641 gcggtgctac cagcaatttg tctgtgtgtt ctacgatttc tcccatcgcc agtgcctgaa
    11701 aaactcttct cgcctctttt gcaatctctg tatcggctgt catcagggaa aggtctgtgt
    11761 aaaggcacgc tgttttttcg ttataatttc ctgttccgat ctgcgtgata tactgcactt
    11821 catcgccggc ttttctggtg atctggcaca gtttggaatg tactttaaat ccatccagcc
    11881 catagatcac atgacatccg gcacgctcca gccttcttga ccactcgatg ttattttcct
    11941 cgtcaaatct tgcctttaat tctacaagtg ccagcacttc cttgccattt tccgctgcct
    12001 cgatcagagc ttccaccact ttactctgtt ttgccacacg gtagagcgtc atttttacgg
    12061 aaaggacatc tttgtcgttt gcagcttcat gcagcatcgt aagaaacggt ttcatgctgt
    12121 catacggata agataaaaat ttgtccccct ccagaatctg atccagaatc ggtctgcctt
    12181 tttcaaactg cacagatttc tgcggaatcc gtttttgata gaacagttcc ggcacgtaac
    12241 gcagtttatc ctggatttca tagacaaatg acagatccag cggtccctca ttatagaaga
    12301 catagtcacg gtcaagatcc agataatgac acagagtact gatcacttca ccatccattt
    12361 cccgtgacaa ctccaaacgg atcggagcca gttttttcct ctgtttgatc acttccacca
    12421 taaaatcacg ataatccaga tcttcgtcat acagtgcgtc tgcgtcaata tctgcatttc
    12481 tggtgatgcg gatcagtgat tttgctttta cacgataacc cttaaagatt tccggcatat
    12541 aatggagaat cacttcttct gccagaatat aagtgccgcg tttgccggga agttctatca
    12601 gacgtttgaa cataccactg ctgcatggaa tgatacccag cttctctttt ccgctctttg
    12661 tctccagcac tacgactgca tagatttctt tatttcgcag aaatggaaac ggctgtcttt
    12721 ttgccacgat cgtcgggaaa atgatcggtg cgatttcacc ctgaaaatag cgtttaacgt
    12781 attcgctttc tttttcattc atcttcttga aatccgtgat gcagatatca tattcttcca
    12841 gttcttccat gatttcatcg taaacgacat ttctgcgacg gttcagtttt ccgacttccg
    12901 caaggatctt tttgatctgc tcctccggtg taagatttgt tttattatca cggatatctt
    12961 tcttcaaaag catctgatct acgagcgatc ccacacgcac cataaaaaat tcatcaagat
    13021 tgctctgata gattgctgca aaattcagtc tttcacagag cggcactttt ttattttccg
    13081 cctcttccaa aacacgttca ttaaatttca gccacgacag ttcccggttc atatatactt
    13141 ttctcattta ttattttgtc ctcctcaata aaatgtgatg cctttaacag ctttttcgtt
    13201 gcgtgcattt aagccagaca cacgtgagac ctgggctaaa tcccataaaa atggcattct
    13261 acctttaacc ataaatttat tttactgcca caagatcaaa tcctctgcaa tgccatgtaa
    13321 aatccatgta aagaatctta ctattcgaaa atgaaacccc actcagacaa atctgttgcc
    13381 cgccacttat aaaagtttga atcttttcca aatcgagtag gaggcggtga ctaaccgccg
    13441 tcctctcaca ccaccgtgcg tac
//
LOCUS       DAWDWH010000030        13420 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_2536_length_13420_cov_15.272877, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000030 DAWDWH010000000
VERSION     DAWDWH010000030.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 13420)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13420)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13420
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2536_length_13420_cov_15.272877"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    964..973
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3992..4001
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6247..6256
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3689 a         2804 c         3544 g         3353 t
ORIGIN      
        1 ttgatccttc ttcttcaatt aagtcttata actttacaaa tacagcgatc aggagatttt
       61 atgatacgat tgattcggaa cagtttaaag atgaaaaaaa agagaagatc tttgaatatc
      121 tgaccggtga aatggagatc gttccattta acgatcagtt gaaacgatat ctttatgaaa
      181 aaaatgaaat gcaggaagca tttcgcagtg tgaccaatga gcagtatgtc gctctgatcc
      241 tggatggatt tgaaaaaaat gactgtcttg cttccgttgg ggcgaaaaca aagcaggaga
      301 tgaagcgtaa ggcgaaccgc tggatcgcag cagaaagtgt aaaacgtgag agtatttttc
      361 agatgggatt cgggctggat atggacgatc aaacaatttc caaatttctg acgctcgtgc
      421 tcaaagaggg agattttgat ttttacgatc cgaaagaaat tgtttactgg cattgcagac
      481 gtaccgggaa atcttatgcc gcggcagaaa aactgctgga agaatatgca gctgagcctt
      541 ccgatacatc tgtacggaaa gatcatatgt gggaggccat gcagaataca ccgaaactgt
      601 atgtaagcac ggaagacgga ctgaaaaaat atctgcatta tattaaaaat ttaaatattg
      661 cagataaacg tgaaaacaga gcaagacagg tatttgagcg tctgtatgag agagcaaaag
      721 agaccattgc aaaacatatg aaccgttatc cggatgagaa agaagttgag ccaagaagat
      781 ttacagcagg agatatcagt gccggaaaga tagaaagtgt gctgtacact gaagtcccgg
      841 taacaaaaaa cggcaacatg aaatcatttg cctgcctgaa aaaacagttt tccggaaaac
      901 gtctgagtcg cgaacggatc agcaaagttc tagccggaaa accggtggag cgttttgatc
      961 tgannnnnnn nnnatcacgc tgatctttct gatctatgca ttagatgatg aaaaacttga
     1021 atcaggagcc ggaccgcgtt tttcagcatt tgtggaagaa acaaacgaac tgcttatgga
     1081 ggcggatatg atggaactct atccggtcaa tccatatgaa gcatttattc tgatgtgtgt
     1141 cgtttcggag gatcttatcg atacttttgc attggtatgg aagaaatcat atgaagaata
     1201 ataatagaat ttccgtaaaa gcagtgtgtg cgagtattac aggagcactt atggaacgga
     1261 atgaagataa tttctgcttt aatggaatag gtatgccgga agtacatgat gatctggaag
     1321 aaaatttaac agcagaagtc ggcggtcagt gggtcgcagt attcgacggg atcggcggtc
     1381 tgccacgtgg agaagaggct tcttatctgg ctgccaaaac gctgctggac gaagaacggc
     1441 catggacgga gcaggacgag cagaattacg aaaccattct gctgcaaatg ctgaaaaaaa
     1501 taaatgataa tatcacaagc tggagaaaag accggaagat cacaaaaatg ggaacaacta
     1561 tgtctgcatt gaagtttggg caggaagcaa tctacggact gtcgatcgga gattcccgta
     1621 tttaccgcat gcatgatgga aaactggaac agctgtcaac agatcatacc tttcgccggc
     1681 caggccatat gaaaagtgct ctgactgagt atctcggaaa agaacatcag gcagaatttc
     1741 cggagaacag ttttttctgt gtgccatata aaaagggcga cagatatctg atctgctcag
     1801 acggactgac agacatgctg gaagaatcca agatcaaaag ccggatggaa cagccggttg
     1861 aagaggccgt gacatctttg atcgaggaga caatccgtat gggagcagat gacaacacaa
     1921 ccattatttt ggcagaggta gagagcgaaa cggatgaggt gcagaatggc tctgatgcga
     1981 tacagagtaa gtcagatgcg gtcatgggca aacagatcga gtatatacag gacggagcag
     2041 agatcacaag cgaagagtgg atcatgcagc tgcagcagga gaaataccgc tcctttggct
     2101 ttgaatacaa aaactggaag aatgaaaatc tgattttcta ccggacggat caggatacaa
     2161 acgaaaaaca ctattacagc aattttgtcg gcattgtaag atgggcagat aaaatgctgc
     2221 tgtcatttcc aaaatctgtg aaactggatc agagcgaagg ctatctgaaa aaactggaaa
     2281 tgctgaaggt ttatgcaaag cttctggatc tttatttggc ggatgtatgg gaagcggcag
     2341 aacgaaatca gaaatgcaaa ccgaaaaaag ctccatggaa tatggtcagt cagagtgtag
     2401 aacagtggtg tgcacagaca gaaaaagagc aggcagatac cgcagaagtg cagatcatcg
     2461 ctgcggtgaa atttgaaaaa gtatacgagt ggcttcttgg ttggctgtat ggcaaccaga
     2521 tcagcctttt cggcgagaaa gtattttttg aaagcaaaat cctggatcaa aaagagatca
     2581 acatcaatga atgtcaaaat aatgtctatt cctggaaggt gtatggaaaa agcggaaacg
     2641 gcattcagga caggacgccg gtctttaaaa atcaggaaaa aaagaatatc ccggatatcg
     2701 tgatcgagat ggataaagaa gacccagaac tgaaaaatat ctgctgtatt ctggatgcaa
     2761 aatattgcgg ctgggatgga gaatcttata ttttgccggg aaacgcagat atatataaac
     2821 agttctttta tcaggaacag tttgtgaagc tctattcgaa gcaagcaccg gaaaaagaag
     2881 tgaagattta taacgccctg attttcccgg attatatggg ggatactgtt cggacaaaca
     2941 aaaacaaaga agggatctta agactctgtg cagtagcagc ctttgatctt cacaaagacc
     3001 gtaccattgg tatctggcag gtaaacctgc agaaactgat tgaaggacgt atcagttccg
     3061 atgaagaggt gcaaaaacgg atgcagaaaa actgccgggg cattacggaa tccttgataa
     3121 aaaccggcaa agacttcctg ccgatgagaa caaaataaaa tccgtaccat tgaaattaca
     3181 gccttcgtgc gtctgtgcgg tatgatagga ccagatcaag acaagaaaag aaaaaacaac
     3241 agatggcata cttgagaagg aggatgcacg atgagagaac aacaggcaaa agacttctgg
     3301 tccgcttatt taaaacttat tttaagaaaa ggtcaggaaa cgaccattgt caatccgttt
     3361 gaagaatttc gcgattcggt cgctaaaggc gggtacgaaa gcgtcagcga tgaagaatat
     3421 gcgaatatct atgatcaggt ggaagaactt tccgtattct ggcagcagcg tccaaaagac
     3481 agtgtcgcac atattgttat cgaggcactc tcaaggacac atctgtacga ggatgaaatc
     3541 caggatatcg ccggtattct cgcagaggca tcggaaagaa ccaactacac ccagcagatg
     3601 ctgcaggaaa tgggagcaga ggaagccgaa aagaggatgg cagatcgaaa aaaagcaaaa
     3661 cttccgccgg tgccgccgga ttatccggaa aaagaatgga tcgaagcgga atacggtttc
     3721 gaaggtgtct acgaagaaac accattcgat gacacagact acagctggac accaaagctg
     3781 atctatcagt atcttggcga acacgtttac ggtcaggaag aggcaaaacg cggagcagcg
     3841 atgctggttt ataatcatct gcaggggcat cgccgcaata tgcttgtggc cggaccgacc
     3901 ggatgcggca aaaccgagat ctggcgtacc ctgcagaaaa agttctcttt catcaagatc
     3961 atcaacggac cgcagctgtc gtgtgacggc tnnnnnnnnn ngatattttc ctcgaagaga
     4021 aaccgcagat gcgggagcat atgatcgttg tggttgatga agcggacaaa ctgtttgagc
     4081 cgatggtcgg ctccggcgga acggactttt ccagaagcat tcagaatgag tttctgaaac
     4141 tgatcgacgg agatcaggta acgttcgtga acgaggacaa ccgaaaagat ccacagacgg
     4201 caaagatcga ctgccgtaat atttcctttg tcttctgcgg aagtttcgaa atgcttcgaa
     4261 acaataaaga agacagatct tctgcgatcg gattttccag cagcacagaa acggcagatc
     4321 tgacttctga agtcaccgaa gaagatctgg tgctgtacgg aaacatccgc cgtgagattg
     4381 ccggccgcat cgataagatc gtgatgctgc agcctcttgg tgcagatgac ttcgatcaga
     4441 tcatgcagag caaaaacaag atttctccgg tcagtcagat tgccggacag tacggcatcg
     4501 aaatttctct ggatgagaaa acaaggcgga tgctggcgga agaagccagt gaaagccagc
     4561 tcggctgccg ctacatccgc tccaaaatcc agggaatgct ggatgatcag atctttgaag
     4621 atccgtatca gaaaaagttc cggctgtcga tggcataaaa tggtagattt ttatttacca
     4681 agatgccaga tatcgtggtc atactcagca atggtgcggt cggaggagaa cagaccggct
     4741 ttggcaatgt tcaccagaca tttgcgtctc catcccatcg gatcgcaggt ataatcactg
     4801 agagcctgtc ctttgcgtac aacgtatgcg ttgaagtccg gcagggtctg gaaccagtcc
     4861 ttgtggatca gctcatgatg cagacggttc aggctttcag aatgaccggc tgccagcatt
     4921 tccggaccgt tgaggaaatc aatggcacgg cggatgttgg catcgttttc ataccactga
     4981 gcggcaacgt aatcattggc agcatagcga tggatgacct gatcggagct ctggccgaag
     5041 atgtaaatgt tctcttctcc gacctgctgg caaatttcta cgttggcacc gtccatagta
     5101 cccagtgtca gtgcaccgtt cagcatgaat ttcatattgc cggtaccgga tgcttctttg
     5161 gaagcgagac tgatctgttc ggacagatcg caggccggga tcagtttttc ggcagcggta
     5221 acgttgtagt tctcaatgaa tacgatctgc agccatttgg atacttccgg atctgctgca
     5281 atcacttttg agagagtcag cagggcatgg ataatgtcct ttgcgatggt gtaggccgga
     5341 gcggctttcg caccgaagat gcttaccagt ggaacggcag gctgatgacc ggctttgatt
     5401 tcatgatact gatggatgag gtacagcaga ttcagctgct gacgtttgta ttcatgcaga
     5461 cgcttggact ggatatcaaa cacagcattg gtgttgactt tcacattctg agttttcaga
     5521 agccagtcgg ccagatcttc tttgttagct ttttttacgg cggtcagttc attcagaacc
     5581 gtttcgtcgt ccgcaaaggt caacagtttt tccagttcgg acgcatcctt gcggaaaccg
     5641 gagccgatgt actgttccag tctggcagtc agtcgcggat cacattccag cagccagcgg
     5701 cggaacgtaa taccgttggt cttgttgttg aatttctccg ggtacagttc ataaaagccg
     5761 tgcagttctg aatttttcag gatttccgta tgcagggctg ccacaccgtt ggttgagtga
     5821 gtgaagtgga tgtccatgtt agccatgtgt accagactgt cctggtcgat gatggcaaga
     5881 ctttcgtctg cagtgcgggt acgggccagt gcatccagct tttcgataac aggcatcagc
     5941 tgtggcacaa cggcatccag ataggcacga ggccattttt ccagtgcttc tgccagaatg
     6001 gtatggttgg tgtaagcaca ggttctggtg acaatttcaa cagcttcttc aaacgcaatg
     6061 ccctttttac caagcaggcg gatgagttcc ggaatgacca tactcgggtg tgtatcattg
     6121 atctggatgg cagcataatc ggcaagatca tgataattgc agccacgggc agcacattca
     6181 gcaaggatca gctgtgcacc ggcagaaacc atcaggtatt gctgatatac acgcaggcgg
     6241 cggccgnnnn nnnnnntcat gaacgatgct ttcgtcgatg gtgtcgaggt cgaacaggtt
     6301 cagggtgttg gtgcggcctt cgtagccggt aactgccagt ttgtacagtc tggcagtgta
     6361 ttctttgcct gccagttcta ccggataggt tatatcggtt ttttctgccc agctgtgtgc
     6421 agtcagccat ggatcgggtt tttcgttctg gatatcattt tcaaaggact gatggaaaag
     6481 tccgaagtga tatcgcagac caacgccgtc acctggcagg ttcagtgttg ccagcgagtc
     6541 cagaaagcag gcagccagac gtcccagacc gccattgcca agagaaggtt ccggttctac
     6601 ttcctcaatg tctgagagat tctttccggc agcagccaga gcctcgcggg cctcctcgta
     6661 cagaccgaga ttgatcaggt tattggataa cagtttgccg ataagaaatt cggcactgat
     6721 gtagtaaagc ttgcggccgg tgacgctctg tacacggtcg gcacttttct gtcgtacgag
     6781 ttccagcaga gccaggtaca gctcctgatc tgtacagtca gccagcggct tttttgtgag
     6841 attctgtaat atttcagtga aattcataaa caaatcctcc ttttgatgtc gttgatatgg
     6901 aaaacgagtt ttgatgtctg tgtttcaaga tataccacat aaaacaatca aaatcttgcc
     6961 tgaaaagtgc aaaatatggt acaatcctat cattaggagg tatagcgatg caaaaacagg
     7021 tttccttgcg ggaaacaaaa cagcatgggg ctgtgcgttt tccgttcaat atttatccct
     7081 gcaccattcc gggagatttc ccgcaggtgg cactccactg gcaggaaagt atggagctga
     7141 tctttgtaaa gcggggagcc gggctggttc aggcaggtgc tgtgacgcat ccggcttata
     7201 tgggagatat tttcattttt acaccgggta cgctccatgc actcaggcag acagagggtc
     7261 agtatatgga gtatgagaat attatttttg atccggaact tctgggtggt gcaggagatc
     7321 tgtgtgccga aaagtatatt cttccactgc aaagcgggcg tctggcactg aacgtacatc
     7381 tgacgccgga tgatctgtat tatcctcagg cggcgaactg tctgaaagaa atcgaagatg
     7441 ccaacagctc caaacttcca ggctatgaac tggtcatcaa gagcggactg ctgcgttttc
     7501 tggctctgct cattctgcat ggaaaacagc agcttccggc ggaaacggcg gataccagac
     7561 ggctgaaaac cgtcctgcaa tggatttcgg aacattatgc ccaaaagatt tgcgttgccg
     7621 atgcagcaga agtatgttct ttcagctcca gccatttcat gcggtggttc cggcagatga
     7681 cggggcagag ctttatcgct tttctgaatg aataccgttt gaatacggct gctgaggcat
     7741 tacagaccac ggatgaaacg gtcctgacga tcgcttcacg gtgcgggttt gagaatctgt
     7801 cctattttaa tcgaatattt aaagcacatt ttggaataac accacgggat taccggaaaa
     7861 gataaacaga cacgaccgtt tactgtatgg agaggaacgg tcgtgtctgt ttctgtcaga
     7921 tcttcggagg agggcagcag ctttcgcggg ggatcagctg gtgtggcaca atgatttttg
     7981 ttgccagaag attcgggttt tcgatatggt taatggtctg gctggcggct tccatgccaa
     8041 gctggtaggg attgacatcg atcgttgtca gccttggact ggtgatgcgg gaaaacagtg
     8101 agttgttgaa cgaaatgatc gaaagatctt tcgggatgct gaatccaagc ttaccgcaga
     8161 actgttccaa taccacagcc agaatgtcat cgctgattag cacggctgta ggacggtccg
     8221 cagatgtgag gagcgtttcc agtgcatcct cataggaatc gttcagagag ttgatctcca
     8281 cacagtcttc ttcacgcggt gtgatcccat gtttcagcaa agacatctgg tagccgcgtt
     8341 tgcgttccgc agaaaacagc atggcgttgc tgacaccgaa ataagcaatg cgacggtgtc
     8401 ccagttcata aagataatcg gtggcttctt ctccggcaag ggcattgtcg ttgtcaatgt
     8461 agatggtctg gttggcggat tgcactgctt tgccgacaat gacatgaagc agaccctcat
     8521 ttcgcagata ggatgctact ggacagtttt ttttagaata gagcaggata aagccatctt
     8581 cctgggaatt ggataccatg ctctgtatcg catccagcac ttctgcatca tcctgcccgg
     8641 tgatgaccgt attcacatag cgtcgctggt tgcagaactg cccgatgccg cggatgatct
     8701 ccagatggaa tgcgttttca tagacatcac gctgagaaga gggcaggata atgccgatcg
     8761 tcttggaaaa tgcctctacc ggttctgtct caaaattggg tacatagccc agctgtgcca
     8821 taatgcggcg tacccgttcc ttggtttccc ggctgatgga agggctgtcc ttgcaggtgc
     8881 gtgacaccgt ggaaggtgaa acaccggctg ctgctgcgac atctttaatg gtaactgcca
     8941 tagtctgttc ctccttggtt ttcattgtgt ctattgtaaa gctattttta tgcaatgtca
     9001 acgctggtat aaggggcggt ttgcatgctc attgcatgca acaaaccgat tcatatattc
     9061 aatttccaca aaaaacctta taaataattg tgaaaaaagt agaaagtttg tttttagatt
     9121 agaaaactct tgtaaaagtt gcgtaatggg attattctag ttgcataaag ttacgcaaat
     9181 acagtgcaat acttgcgtga aataatgcaa aaagataagg aggaatatca tgacagcaat
     9241 gaaacattca accgtcgtga cagcaccctt cagcggaaac attgttccgc tgaacaaagt
     9301 cccggatgaa acctttgcaa gcggtgtttt gggtgaaggc atcgccattg aaccgtctga
     9361 cgggttgttt tgcagtcctg taaacggtac ggtggaaacg attgccggaa caaaacatgc
     9421 cattggcttt gtggcagatg gcgggctgga aattctggtt catgtgggtc tggaaacagt
     9481 cagtcttaat ggcgagggct ttgagattct ggttaaagag ggtgacaaag tcagaaaagg
     9541 tcagcctgta gcaaaagtag atcttgacct tattcgtgag cgggggctga aaacgattac
     9601 gtctgttgtt ctgacaggcg gcacagaaga tatggagctg cattgtgcaa gcggaactgc
     9661 tgaagcaggt aagacatcga ttctgacact gaatacaaaa gagcagccgg ccggtgcagc
     9721 cagaacagat gctcagccga ccgggaaaac caaagcagag aagaaagtga agaaaaaagg
     9781 catgattaac tttgatttcc tgcagaagct gggcaaagta ctgatgcagg tcatcgctgt
     9841 tatgccggca gccggtctga tgatcagcct tggtaagctg gtgcagatgg caggtgcaga
     9901 tatggcagtt gttatgacca tcggtactac catggagaac attggctggg ctgtgatcaa
     9961 taacctgcac atcctgtttg cagttgccat tggcggcggc tgggcaaaag aacgtgccgg
    10021 cggtgcattt gcagcagtgc tggcgtttgc tcttatcaac gtcatcaccg gtaatatttt
    10081 tggtgttacc agtgccatgc tggcagattc cagtgctgtg acccatacgc tgttcggaca
    10141 ggagattgct gtcaatggtt acttcacttc cgtactcggt gccccggctc tgaatatggg
    10201 cgtattcgtg ggcatcatcg ccggttttgt gggcggtgtg gcatacaata agtactataa
    10261 cttccgtaag ctgccggatg cactggcatt ctttaacgga aaacgtttcg tacctatgat
    10321 ggttattgtt tactccgtgg ttatttctct ggtgctggca ttgttctggc ctttagtcca
    10381 gaccggtatc aacagttttg gtatctggat cgccaattcc tctgcaactt ctccggtact
    10441 ggctccattc gtctatggta cactcgagcg actgctgttg cctttcggtc tgcaccatat
    10501 gttgacgatt cctatgaact acacctcttt cggaggtact tacaccatcg ctaccggtgt
    10561 caacgcaggc agccaggtgt ttggtcagga tccactgtgg ttggcatggg caaatgacct
    10621 tatcaacttt aaaaaatcag gcgatatggc tgcttacaat aatctgctga ctacggttac
    10681 tccagccaga ttcaaggtag gtcagatgat cggtgctacc ggtttgctgc tgggtattgc
    10741 actggctatg taccgccgtg tcgatgctga caagcgtgca aagtataaat ccatgttcat
    10801 ttccacagta ctggctgtgt tccttaccgg tgttaccgag cctctggaat tcatgtttat
    10861 gttctgtgca atgccgctgt acgtagttta tgcgattctt cagggatgtg cttttgcaat
    10921 ggcaggaatc atacacctgc gtttacattc cttcggcaat ctggaattca tcacccgaat
    10981 tccgatgtcg cttcaggccg gtctgggcgg cgatattatc aactttgtga tctgtgtgat
    11041 agtatttttt gcaatcggtt actttgtagc atattttatg atcggtaagt tcaaactggc
    11101 aactccgggt cgtctgggca actataccga tgcccatccc tctggcagat tattgtccat
    11161 gttccggcaa cgggatattg ctgaaagagg acaaccatgc ggcagctgtt gcacagatgc
    11221 taaaggaaga gggcgtacat tattactgga gctggcttcc ggcaaaaatc ggctacgaca
    11281 agtatgatga aggtatggca attttcagtc gtatgccaat cactgcaaca gaaaatctgc
    11341 tgctgagcag gacagacgat tatcattact ggaaaacgcg tcgtgcactt ggtatctgtg
    11401 caggaaatgt ctggtactat gcggtacaca tgggctggtg gaaggatgaa gaagagcctt
    11461 ttctcgatca gtgggaaaag atgtcccgtg cagcagccgg aaaacagatg gcatttctgc
    11521 tgggtgattt caacagtgaa gcagcggttc ggggagaagg ttatgatctg atcgcccggg
    11581 acggctggca ggatacctac tgtctggcac agcataagga tgatggttat acggtggtgc
    11641 aggccattga cggctggaga gatactccgg atgcagcaga gaaaaaacgt atcgatcaga
    11701 tctggtgctc aaaagttatt ccggtaaaga gcagccgagt tgtatttcgc ggaacacagg
    11761 aaccacaggt gtctgaccat gcaggtattc tgatcgaatt ataaaggaag gtaaaaaaga
    11821 tgaagagaag tgcaggtatt ttgctgccaa tcagcagtct gccgtccccc tacggtatcg
    11881 gctgtttttc acaggaagcg tatgattttg tggactggct caaagaagcc gggcaaacat
    11941 actggcagat tctgccgctg ggtgtgacca gttacggcga cagtccttat cagagctttt
    12001 ctgcttttgc aggaaatccg tattttatca gtctggatga actggtaaaa gagggtgtgc
    12061 tgacgactgc cgagtgcaaa aaggcaaatt ttggacgtaa ggcagatgac attgattaca
    12121 gttgtcttta tatagaaaga aaccgtctgc tgcggctggc atattcccgc agtgatatcg
    12181 gccacaacga agcgtttctg agattctgtg agaaaaatca gtggtggctg gatgattttg
    12241 cactgttcat ggcagtgaaa gatcgttttg aaggcagacc atggatcgag tgggcggaag
    12301 atatccgtct gcggtggcag aatgcgatgg attactatcg ccgggaactg tattatgaag
    12361 tggagtatta taaatatctc cagttcaagt ttgatgaaca atggagcaaa ctgaaaactt
    12421 atgcaaacag caaaggaata cagatcatcg gcgatattcc catctatgta gcgctggatt
    12481 ctgcggacgc atgggcaaat cctggtctgt ttcagctgga tgaggagaac attccgactg
    12541 cagtggcagg tgttccgcca gatggatttt cagcaaccgg tcagctctgg ggtaatccgc
    12601 tctatcgctg ggaagtgcac cgcgataccg gttatcagtg gtggatcact cgcctgtggt
    12661 actgcttcga actgtatgat gtggtacgta tcgatcactt ccgtgggttt gatgagtatt
    12721 tttcaatccc ttatggcagt gaaacagctg catcaggaca ctgggaaaaa ggaccgggca
    12781 ttgaactgtt ccgtgcggtg gagcaggcat tgggcaaacg ggaaatcatt gcggaagacc
    12841 ttggctatat gagcgacacc gtgcgtaagc tggtgcgtga cagtggattc ccgggaatga
    12901 aggtactgga atttgctttt gattcccgtg ataccggcag tgccagcgat tatctgccgc
    12961 ataactattc cgtgaacagt gtggcctaca ccggaacgca tgacaatgaa acactggtgt
    13021 catggtatca gaccatcacg gctgccgaac gtgctatggt gcgtgattat ctgtacgact
    13081 atgccacccc ggaagaacag ctctataaga gcatgattgc actggttctg cgaagtgcgg
    13141 ctgccacctg catcattccg atgcaggact ggctgggact ggacaattcc gcccgcatca
    13201 acaaaccttc tacggttggt gagaactgga gatggcgtct gaaaaagacg cagctgacca
    13261 aaaagctcca aaaagaaatt tgccgtatga ctacccgata cggacgtatg aactgggaat
    13321 agcacttccg catcagatgg aacagcatat cgtgtgcagg atctgattta tctcagtcga
    13381 gaagactccc acttctgcaa gtggtgagta ataacaagtc
//
LOCUS       DAWDWH010000031        13380 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_2542_length_13380_cov_12.632195, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000031 DAWDWH010000000
VERSION     DAWDWH010000031.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 13380)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13380)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13380
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2542_length_13380_cov_12.632195"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3674..3683
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10442..10451
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11348..11357
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3025 a         3401 c         2568 g         4356 t
ORIGIN      
        1 caacgcctgc tgctgcggat acaccgaact cttcctcgca agcttttact aaatcattta
       61 actctaatac agataactct ttgatagctt cgataaattc agctgttgtc aattttgcca
      121 ttttattttc ctccattatt tttatttttt caggcggcat tttctatgcg gcctctgtga
      181 tgattcttat tcttatgctt cttcctgttt ctctgcaatc tggctgataa cgcgtgcaaa
      241 gtttgcgatc ggagactgga tgcttccaag cagtttgccc agcagttctt ctcttgaagg
      301 gatgcttgac agtgctgtga tacctgcctc atcgtagtaa gcgtcttcca caacacctgc
      361 aacgagtttc agtgctttca catcttttgc atatttagcc aggattcttg caggagcagt
      421 tgcatcatct ttgctgatag cgattgcgtt cggtccttcc agatgtttgc aaagttcttc
      481 acaaggtgtg cctttgaatg caaacttcat cattgtgttt ttgtacactt tgtaagaaac
      541 accggcttct ctcagctctt tacgcattgc agtatcctgc tctacggtga ttccgctgta
      601 agtaaccagt acgatggaag ccgcatcttt gatcgcctca ctgatctctg ccacgatcgg
      661 ctgtttctgt tctacttttg ccattaactt ttacctcctt atagattctg cccggtgtgc
      721 cataaaagcc caccactgaa tattctggga ggaattcata acaatgaaaa acctctctgt
      781 caaactaaca gagaggttct gtaatcattc ttttaagaat tcttcactcc tcggcaggcg
      841 ttttaacctt atgcctaacg gcacctgctg tctctggcag ttttctgtaa aactatagca
      901 catctgccac tgttatgtca aggagttttt acatttttct gattaagaat ttttgtattt
      961 ttttctgacc acttttgacc agttgctctg cattttctta ccgcccgctt ttttataagt
     1021 ccggatcttg taatagtaag tttttccttt gcgtgccttc ttgtcaagat aactctgggt
     1081 ctttcccttt ttcacaacat ggatcttttt atatccgctc tttttctttg tggagcgata
     1141 aatataatat ccatttgcac cggatacttt tttccatgtg atccggatcc cttttttcgt
     1201 tgcttttgct tttacatttc tgacagcttt cggtctggtc acagtcggtt ttgccggtgc
     1261 tgccggcgtt tgagtttccg tctgtttttt atcatccgac ggtttgttta ctgtttcact
     1321 ttccgattct ttttctgatt ctttttccga ttccgttgtt gtctctttct gcgtttcact
     1381 ctctgtttcg ctttctttct gtgcatctgc cgggattttg aatgcagtaa cagaatgcgg
     1441 agccaggctg tattttgctg ttttctctgt tacggaaatc tttgttttct gtacatctac
     1501 tttatttgga tttgcctgtg tgttttcatc cagcacatca tccgatgtca gataccatat
     1561 ctgaagatct tttcctgtca ggtcttttcc gtctatgatc agatttacat ctgttctttc
     1621 atctctgctg ttattcacaa ccgtaagata aacattcccc tgttcatctt ttgaggaaat
     1681 tgcttttaca agcggaacat tgtacccctg ataggtataa caggtcacat ttccttcaat
     1741 tttctggcca atctgcgttg ttcctgtcat attattgaaa atggaaaaca tttttgcaga
     1801 aggggtcgac acaaaatcgg tcgttccatc gttttgtttg attgcctgga tcacacactg
     1861 agagcccgat ccaaggttat ctgctccata tggataatca accagacaat gtttgttgat
     1921 ataaggtgtt ccaaagccaa tgtaatcaat catttcattt gcaatgtaaa cagcatgtcc
     1981 gattccacgg acaaactggg tattatggtt atatacgcca aactcagaca gaaccggcac
     2041 tttccccatt ccaggtgcat actgattcat cgcatccacc agtgctttta cacggggcat
     2101 attgtactgc aatgaccgtc cgagcacttt ttcatagaac tccggatcat tgtctgcaat
     2161 gactccaccg cttgtatcac tgtacggatg gataacaaca ccatcgtaat aattattata
     2221 gcctttctgt gccattttct gcacaaaaga aacctgattg ccgcttccgc agtatacctg
     2281 gatctccatc cccagttcat cggcaatctt gttcatcgcc tgataatacg acacaaatcc
     2341 atcacggtca ctctggtaag atgcggtgat tgcctttcct gactgtggaa tatttccatt
     2401 tattccatca ccaaatgtaa ttttacctgt tttgctgtct acagtgcaga cattctgtgc
     2461 cccctttcct tccagggaat ccacgatctg ccactgtgtt ccgcctacat agacagccac
     2521 agaattttct actgccggtg tataacgtac atagcgaacc tgatttgctg ttccatcact
     2581 gtttgtggcc cgatctctcc agtcctcttc cttaaccgtc ctggtattct gaccgaaagt
     2641 cattctgccg ccgtcaatat aagcatccat ccagtcagtg tttcctctgc catccatcca
     2701 gtaggtctgt ctgtaataac cgatttcatt tccgatttca aaacgtgtta caccataagg
     2761 tttcggatgc ccattggctg cacgcacttc tgcccagtct acgccaccgt ttgggttcgt
     2821 cacatcaccg tctgccggtg catttaagta ttcgatcaaa tctgccgcat ctgctgcact
     2881 tccctgaccc ataccataaa cccagaccgc ctctgctttg atatcatcgt aaatccagct
     2941 cattgcctca tccagcccga aattcggtgt gatcggatca gatgtttccg gaagtccatg
     3001 tactgttttc tttcgctgct ctgcaggtcc gatgctgcgt ttccagtcaa acagattgga
     3061 taccgttcca cctggataac gtacagaacc aaaatgtgct tcttttacat attcattaaa
     3121 tttagcttcc actttctttt ctgatgcgtt ccatgagctg taagcattat tatgataacg
     3181 atgattgatg ccaaacattg cttttgagat ttcacgcact tctttggaag tatccaccgt
     3241 aatgtctatg ttcgtgacag cattttcaga ataaaccttg taaatactct ggatctgtgc
     3301 cggtgtcatc acttttttat aataacgcac ttcatccata tatcctttga aacctgtttt
     3361 ttcagtagca ttcttatgat ttccgatcag gagatccgtg agtgcatcta cataactacc
     3421 gtccaacgtg ttttcgttta ccagttttcc attgatataa aactgtgctt ttttcgccgt
     3481 atggctgctt gtaaatgcca catgcaccca tttgcctccg gctaccgggc tcttgctcag
     3541 cacatcgctg gctgtcacat aagtcccaag tttcatatta gaatccagat agagaatgct
     3601 ccgtcccgcc cctgtctgct ggaacagata ttttttgtcc gtgctgccgc caaacatatc
     3661 gtcatcgcat ttgnnnnnnn nnntcgcatt tgatccatgc catcagcgtg acatcatccc
     3721 aagctgcatt gactgcatct ggcagtttca gcgaagatgc agatgttcca tcaaaatatg
     3781 ccgcacttcc cgaaatacct tcgctctctt ttctggtaac tccgctgccc tgcacttcca
     3841 ctttctgggc agactgtgat gcactgttcg tggtcagggc atcctcaaaa ttcagataaa
     3901 cagacagacc ttctctcagg tcatccgctt cttccgccgc aaacacttcg ctataattat
     3961 aaccacccgt gcccggcact gctgctgcag taacacataa cgccgtcaga atgcatgctg
     4021 ttcgtttcgc catgctgctc catgttcttt tctttttcat agtaacctcc tgtttttaat
     4081 tttaagaatc cccgtcttcc tgctgcaagc tgcacgccat acatcgcatg gtctgttttc
     4141 ccatatgctt ccgcagtctg acagaggctg tatgcagatt tactgaatca aatctgcttt
     4201 tttgtaagat cagatgaaaa atccctctca ttctttcgca tctgtttaca tataatatca
     4261 tattaccata aagaatcaga aaaaaacagg tgtattttag ttattttata taatgatgtc
     4321 aaaaaaagac cgccataccg atttctcgat acagcggcct ctgctcgcct tacaaacttg
     4381 aattaaacca gttttaccgg attcagtttt acgcccggtc ccatagtagg agcgattgta
     4441 acacttctca ggtactgacc tttcagtgtg cttggttttg ctttattaat tgcatcaata
     4501 agcgtctgga agttttcgcg cagtgcatcc tcttcaaagg aagcttttcc aagtggcaca
     4561 tggataatgt tattcttgtc caatctgtac tcaatcttac cagctttgat atcagcgata
     4621 gcttttgtta cgtccatagt aacggtacca gccttcgggt ttggcatcag tcctttagga
     4681 ccaagtacac gacccagacg acctacaaca cccatcatgt ccggagttgc tactactacg
     4741 tcgaaatcca gccatccttc attctggatc ttcgggatca gttcttctcc gcctacgaaa
     4801 tcagcaccag ctgcctgagc ttcgtctgct tttgcatttt tagcgaatac cagaacacga
     4861 actgttttac cagttccatg aggcagaact actgcaccac ggatctgctg atctgcgtga
     4921 cgtccgtcac atcctgtttt gatatgagct tcgattgttt cgtcaaattt tgcaacagca
     4981 actttcttta ctaaagaaat tgcttctgga atatcataga gagttgcacg atcaactgct
     5041 ttcgcagcct ctacgtattt ctttcctcgt ttcatattaa ataacctcct ggtggtgttt
     5101 gcgggaatat ccctcccacg ttttttcgtt ttcgtttacg gaactgcaag cttattgtga
     5161 agcctgtatg aaatgaatta gtcttctaca gtgataccca tagaacgagc tgtaccagcg
     5221 atcatgctca tagctgcttc tacagatgct gcgttcagat ccggcatttt catttcagcg
     5281 atctctctta ctttatcttt agagattgta gcaactttgg tcttgttcgg aacaccggaa
     5341 ccagatttga tgttgcatgc ttttttcagc agtactgctg ccggcggagt ctttgttacg
     5401 aagctgaagc ttctgtctgc gtatactgtg ataacaacag ggatgatcag atcaccttta
     5461 tctgctgttc ttgcattgaa ctcctttgta aactgaacga tgttaacacc atgctgacca
     5521 agtgccggac caactggtgg agccggtgtt gcctttccag caggaatctg taatttaata
     5581 taacctgtta ctttttttgc catcttaggc acctcctatg tggtattgtc gggggtttcc
     5641 ctcccacttg cttcggtttc cgattgttcc gaagcggttc tctgttactt acaacattgc
     5701 ggcatcggcc acagccggtg acgcatacca aaaatattac atttttttga tttctgtgaa
     5761 accgatctct accggagtct cacgtccgaa cagttcgaca ttgattgtca cgatctgttt
     5821 acttggatta atggactgaa taacgcctac ggtatctttc catacacctg cggtgatggt
     5881 aaccgtatcg ccttcttcga aatcaatctc aagttctgca ggtttgattc cgagcggtgc
     5941 catttcctcc tctgtaagcg gaactggctt ggatccaggt ccaacaaagc cggtaacacc
     6001 tctggtattt cttacgacat accaggtatc atcattcatg atcatgttca gcagaacata
     6061 gcccggaaac agctttttca gaacctgctt cttggcaccg ttcttcactt ccacaacttc
     6121 ctgcatcgga actcgaacct caaggatctg atcctccagg tgacggtttt caattgtttt
     6181 ttcaatgttg gcctttacct tgttctcata ccctgaataa gtatgaacta cataccagtt
     6241 tgcatctgac atattatcac cctgtcctta ttttactaag aaatcaacac cgtactgtac
     6301 gatcatatca atgatggtga tcagaacgcc cagtactacc gaaacgctaa ggactgctgc
     6361 cgtctgcttg ccgatggatt tctgatccgg ccagataatt ttgcggaact cagctttcag
     6421 accgtcgaac cagctttttt taggagcttt ttctttcttt tccttttcgc tcatataatc
     6481 acttatcctt tcgcacccgg cttatttggt ttccttgtga ttcgtgtgtt tcttgcagaa
     6541 tctgcaatac ttcttggttt ccattctctc aggatgagtt ttcttatcct ttgtcatgtt
     6601 gtaattacgc tgtttgcatt ctgtacatgc caatgtaatt cttgtgcgca aaacttccac
     6661 ctcgctctct ttgttttttg ctcacagcct ttatgggctg atgaatttta ggcataaaaa
     6721 aaagacctac ttccattcgc cagaccagtc tatcatgaca ttgaaaagaa gtcaagtctt
     6781 tcttgtgttt ctgacacttt tttcttgtgt ttctgacact ttttttgcca aatacctgac
     6841 atacggcaag tatcagaaaa gaacgggaaa tacttccgca ttcccacagc ctgacttcca
     6901 tttttcaagc acagcctgat aaacttctgc ctctgccgca tgcatccggc agagatattc
     6961 cggacatcct ttttcttctc cacattcggc agcgatcatc gcaatgcagg gatgacagat
     7021 ttttttgttt gcacaatgta tacacgccgg agaacagcat actttttctg tctgtgcaac
     7081 cagttctttc caggcttctg caaaagaacg ctcttttaaa gatgtttttg ccgaagcgta
     7141 catgccgcag ttgaccagat taccctgcca gtccacccag aaagaacagt gtccggcccg
     7201 gcagtgcatg gtcatcacat tttcatccgt catctttttg tcttcacatc ttttttcttc
     7261 gtcagaattt tcccgaaacc gttcctcgcc tttttggaat ctgccaaacc gctcagcctg
     7321 ccgccaaaac caatcccctt ctccggtcag aaaatcggtc tttactctgg caagtgcggc
     7381 ctcttccggg gagaggcgtt cattttgtcc gaccattact gcgtttcggc ggatcggcgg
     7441 aaacatatag gttgccgcct ggatcggtat ctgacactct tttgcataag aaatcatact
     7501 ctccagatcc tgtacattct gcggtgtaat gctggcgttc aacttgatcg gcaccccata
     7561 ctgtttcagc catcggacag catttttcac tttcagaaag gattcgccgt ttccgcagag
     7621 cttctgataa gtctcttcac tcgccccgta aagtgtgatg ttgatcctgg tcggtgtgtg
     7681 cctgccaagc cacctggcca tattctgatc gatccgggat gcattggaat tgatgctgac
     7741 ctgcattcca agatcctgca ttccggcaaa gacttcctga aaatcttcgt acagaaacgg
     7801 ctccccgccc gtcagaagcg gaaacagcaa accacatttt gcggcatctt ctgcaatctc
     7861 aagccacctc tttccatcca tcagtccgcc tgctttctcc acttctgccc tgcttttacg
     7921 gacataacac attttgcagg caaaattaca gatcgggagc agttcaaatg ccccagtcag
     7981 tgggattctc tccgctcccg cttttgccca taaacgcctg ctgatcgttc cctgcatatt
     8041 ttctcttctc cttcctgttt aagcatacgt tatgtatttc ttgtgacact cttccgtata
     8101 ccttacttct gtactcgctt cacattttgc atgtctgaat aaatttcatt ttatcttttc
     8161 ctacagatat cgtccatctt caggattttt tccaggcacg caactgcctg ctcatccatc
     8221 gttgcagtca gctggtagac tggcatttct ttcagcaaca aaaacagatt ctctgccgcc
     8281 tggtttctgg cattttcact ccaccagtcg atcacaagct gcggcatcag ctgtgccatc
     8341 gctttccctg cccgcagtct ttcgatgctg ctctttccat tctgcgactg ttttaccaga
     8401 acgatgcatc ccgccacatg cacttccccg cttcgcaccg gctgtgatcc atccatcgga
     8461 tagccgtagc tgtgccagtt tccgtcctgt tttcgcagaa gtgtccggtc attgcagatg
     8521 atcctggcat ttctgaacct ctcccagagt tttgcctgcg tggtctttcc cgccccggat
     8581 ggtccggcaa ataaaatccc ggtttcctga tatgtaatct gtgccgcgtg aaaaaacagt
     8641 acaagaaact gttgaaggat ggcccgcatc ggaaaataac gcaggatttc accaagactt
     8701 ttcggtgcaa ccggaagcag ccgttcctgt atcctgcacg aaacatgtga aaaagtatcg
     8761 tcgtattcca cgcaggaaat tgctcccttt tctccctccc acacaataca gtaatttttc
     8821 tcttccctgc gatagtaagt ctggatcaga tcttctccaa gctttaccgc agaacgaagc
     8881 ggcggcttta cccacttcca ggaaaccgcc gccagaatat ctgtctcttt ccatttttca
     8941 tcctgcccgg atataccgga tctttcccgg attataaatg gaaggaacac atcttcgatc
     9001 tgaatttctt tttcacagtc cagacagatt ttcacgccgc caaggcataa catgatgtgc
     9061 atccccggta cctcgctttt tctgtttctc tttgtgttca ggatgtccag taaccgccgc
     9121 ctgctgtcgt gtaacagtcg cacgtatctt ccgaataaaa ctgtacattg tgcacacttc
     9181 ttgcagcact gtatgtctca aacggatcga tgtaatcgct gcactggctg tatccgttca
     9241 tttgcggacc gttgtggacg accgtttcac agttgctggc gatcgaagca tccagctgat
     9301 agcgttccac ttctgctttt ggagtttcat acgttttctt catatccatt ccccttcccg
     9361 tatgtctgtt tctttatgat cgtaaactcg gcatttccgg ttactttgat attggttgcg
     9421 gaaaccagtc cctcttcggc tttgatctca aatgtagcta tcgtgcttcc gtctttttca
     9481 ctgatatccg cacattttga aatttcataa taacagtctg ctgcattttt aagttccagg
     9541 atctgtccgt tgactgagac ttttgcattt ccgaacggag cttttactcc aagataaagt
     9601 ttgttcgtat ttggatccca gtcttttaaa gagaaggtta cgctctgatc ttcatcaaga
     9661 taaacctctt ctttcggccc attgctctta tattcggaag cttttttaat cttaccgttt
     9721 gaatcggtaa ataccacaaa tgtttttcca tcccactgga tctcgccctt ctcatcaacc
     9781 tccgtataat cggagagaag tttctgacgc agtgtaactg ccgtcatatt tgcttcacca
     9841 tcctggttgt aggcctgtgc tgcaatatct gcatgttcat ccgtctcatc aagcggctgc
     9901 atcacacgga tcccatccag ccagaaatct gagccaaaac gatttcccgc ctttgcaaca
     9961 gacacttcta cattatatgt gccatattcg gaagtattcc aggtaaatac cggaatattg
    10021 tataaagtga tcttgttttc actctgataa atggtgttgc ggtatccatg atagatttct
    10081 ctgccttttg catctctgat cacgatccgc agatagccgc tgttattgga tgtccttgca
    10141 aagaaagaag ttccctttcc ggtaaaggtg taagtaaatt ttgctgcacc tttttctgtg
    10201 cttacataac agctagaacc gttgctgtct tcgctgtcac ttaaataagc aacatctgaa
    10261 ccatacggac tgtcaccggc atccccgact acgcccggtt cctggtaatc ctcctgaggt
    10321 gttccctcct gttcccagtt tccgtttgga tattctgcca ttccgaaatc ttcttcataa
    10381 tacatgtcgg tcgccggaat gatataaacc tttgcctgtg cctcctgatc atctacaccg
    10441 tnnnnnnnnn ngtttgtact ctttactttt gcagtataat ttaaaacttc tacgccggtc
    10501 agctgttttg tcagcgtgta atggattttt ccgtctttta cttcggccgt tccatatttc
    10561 agagaattgc tgtcgatttc aaccagttct ttgctgcctc cgactgccag atcatttacc
    10621 agaggatcaa tttcaaccgg aagtccgtag tccagaacaa tataatcgtc atttgcctca
    10681 aaagatgccg tttctttgtg cagcatttca ttttcacagg tgaacgatac ctgatctgct
    10741 ttgtctgctt caccttttgc agtcacgctc atactcgttc cggcactgtc cgaatccacg
    10801 acatggaaac caccggctgc ttccgcctga taactccatt tcggagcgat ccagctcgct
    10861 ccgttgtccg cctcggtaat ataataggtt ttgccgtcat cttcatccat cgttccgatg
    10921 aatttcgcag tctgaccatt tttcagtgca aaccttccgt ccgttccggt cattctcgta
    10981 tctacatact gattgttgat gtcatagaga ttgtaagttg catctgacac tgcttcgccg
    11041 tcacggtaga gcgtaaaata aaaatctcgc tggctgatct gtgctaaaat actgtcatcc
    11101 agttttattc ctttactgtc tgttgttttg actgttttat gcaccgaaac ggtatctttc
    11161 attggcagat tgaatttgat tttacagttg gaagctcctt ctccacgttc cagataatag
    11221 acggtcagcg tatgctgatc ggtcgctgca aatgttttta acgtggtatc aaaaataccc
    11281 gcaccttcag aatcccggaa cacttttttt gagatctttc cggctgttga tcttcctcct
    11341 ttaataannn nnnnnnntgt cttattcgtc tgtgccgtgt cattccaggt cagattcgtt
    11401 tcacttgctg ctgttccaga aaaatacgct gaatcctgac ttgcatcaaa tgtataatat
    11461 ccattttcat atacaaacgg cagtccgaca ttcgtgtaaa cttcttttgt ttttgtgtca
    11521 ccggaatcaa aaatgccgcc gtccggaacc gtaaaatgaa tgtttccatt ttgcatgtca
    11581 gaacccacca ggccgctgta aacataaccc ttctgactgg tctgcgtgga tgagatcgta
    11641 ttatttgtcg tcagcagacc ggtccaggta ttccaggtgg catagctgct gccgggagca
    11701 ccattcgaaa aatagatacc ccgaagaact ctgctgttcg gattttctgc cttgtcttcc
    11761 tgataaaccg cctgattgat cgtgtcctga tcataatcat aaagcgttac cggaaaataa
    11821 cggatctctt tttgtgatgc cgcctgcgaa attgcttggg ataccgcttt tggcacactg
    11881 tcaaaacttc gaaatgctgc ctccgcctca gatggcatgg cggtgaccgt aactacggtt
    11941 gccagcgtta ccgccagaat acgtgatact ctttttctca taattttcct cctttttctc
    12001 aaaacatttt tattttccat aaaaaagagc agagatctgt gcctctttcg cacatttctc
    12061 tgctctcgtt ttcacgatcg ttgctttatt tttatatttc tcacctgtat tttataaaga
    12121 tgccatacgg attgttccgc acttcacacg cttttattct tactgtactg ctttgaatgc
    12181 ttcgtccaga tcctggatga tgtcatcaat gttctctgta ccgatggaca gacggattgt
    12241 gtttggtttg atcccctgat ccagaagttc ttcctctgtg cactggctgt gtgtagtggt
    12301 tgccggatgg ataaccagag attttacgtc tgctacgttt gccagcaggg agaacagctc
    12361 cagattgtcg ataaaatctt ttgctttctg tgcgtcacct ttgatctcaa aggtaaagat
    12421 agagccgccg ccgttcggga aatattttgc atacagctct ttctgtttcg gatcatccga
    12481 aacagacgga tgatgtactt cttctacctg cggatgattc ttcagatact gtacgacttt
    12541 cagtgcgttc tctacatgac gctctactct caaagacagt gtttccagtc cctgcaggaa
    12601 aatgaatgag tggaatggag agatggttgc accggtatct ctcagcagga ttgcacggat
    12661 ttttgtaaca aatgctgcag gtcctactgc ttttgtaaag ctgatgccat ggtagcttgg
    12721 gttcggttct gtcagagaag cgaatttacc ggatgcctcc cagtcgaatt taccgctgtc
    12781 tacgataaca ccgccgatgg ttgttccatg gccgccgatg aattttgttg cagaatgtac
    12841 aacgatatct gcaccgtact caatcggtct taccaggtat ggtgttgcaa acgtattgtc
    12901 aacaaccagc gggattttat gtgcatgagc gatctttgcg atagcttcaa tatctactac
    12961 gtctgaattc gggttgccca gagtttcgat ataaacagct tttgtattat cctggatagc
    13021 tgcttctact gcatccagat caaagatatc tacaaaggtt gttgtgatac cgtactgtgg
    13081 cagtgtgtgc tccagcaggt tgaaggaacc gccgtaaata ttttttgcag atacgatgtg
    13141 gtcaccgttc tgtgccaggt tttcaatggt atatgaaata gctgctgctc cggatgctac
    13201 tgccagtgct gcaacaccgc cttccagagc tgcgattctc ttttcgaata catcctctgt
    13261 cgggtttgtt agtcttccgt agatgttacc ggcatctgcc agaccaaatc ttgcagctgc
    13321 atgatcgcag ttacggaata cataagaaga agtctgataa atcggaactg cccttgcatc
//
LOCUS       DAWDWH010000032        13153 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_2582_length_13153_cov_12.662086, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000032 DAWDWH010000000
VERSION     DAWDWH010000032.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 13153)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13153)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13153
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2582_length_13153_cov_12.662086"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1633..1744
                     /estimated_length=112
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10525..10534
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4052 a         2715 c         3029 g         3235 t
ORIGIN      
        1 tgcaaacact attttaaaat gtgcggcatt tcagttcttt acacctgccg ggaaagcaac
       61 ggtcaatttt tatttgagat tcccaagccg gatatgtttc tgcaaaattt catcgatcac
      121 ttcaaaaagt tccagggatc cgtcttttgt tttcttctgt cttgcataaa cctgtttggc
      181 actgacaccg tgttctctcc agtcgccacc gccattgctg ttattgagca tcagaggctg
      241 cagattgtcc atcgcatgtg caaatcttga ttccggcgtt tcatttgcct caaattcatc
      301 aaacagtgcg gaaagctctt ttttctgatc atcaggaaga agagaaaaga tccgttcttt
      361 tgcggcttcc tcccgggctt tctgtgtttt gagcccttcg gcatcatacg catacgtgtc
      421 tcctgcatca atttccacaa tatcgtggat caggcacatg atcattaccc gttcaatatt
      481 taccctttca tttgcatatt cccgtagcag atatgccatg atcgccatat gccatgcatg
      541 ttccgcatcg ttctcattgc gtccgtgacc ggacagatgt gtctgtcgga aaatattttt
      601 ctccttatca atttccagtg cgaaatccag ttgtttctgt aatctttcgt ccatgtttta
      661 cctcctcaga tatatgaaat atttttggcg tattcatacc atttttgacg tttgttccat
      721 gctgcctgtt ccgtttttct tataaaccca tcgttatttc aaatgtcgtt ccgccctgct
      781 catttccatg aacttcgatc gttccgtcat gcacccggac aatctcacgt accagtgcaa
      841 gcccgagacc tacaccgcca agttcccggc ttcttgattt gtctatccgg aagaagggtt
      901 caaagatctg ttcccggaac gattcctcaa ttccgtttcc cgtatctgcg acagtaagaa
      961 caacgctatt cttcatgcgg actgctgata cggtaacgtt tccattggtg tgattatatt
     1021 tgatcgcatt ctccacaaga ttataaagca tccggtagat caggatatca cttcctgtca
     1081 ggatcagatc agcagttttg ttttctgctt tttcatccct caggttctgg tttttttgga
     1141 tcagttcaac gtttttttcc tgtgccagag gttccagatc agtcagtact tcctcaatca
     1201 gactgtgaag ttcgatcttg tcattacgtg ccacgctctg cagctcgctc atgtcaagaa
     1261 gtgtccggac cagcctggta agacgctcgt tctgttctgt taccatctgg atcgtctctt
     1321 gtgctgcagc gtcacactca ggatggcttg ttgtgtgata gagatctagc tgtgcctgga
     1381 ttaatgccag aggagtccgc agctcatgtg ctgcatttcc ggtgaactga cgctgcgtct
     1441 cgaatgattc cgaaagccgc agaagcattt tattataaga actgcggagt ctgtccagtt
     1501 ctgtaatctt attttctccg atcgtacaat ccgtcagatt ctgtgcctga actttttcta
     1561 cggtttctga gaattctctg agcggcttca gtgcccggcc gctgacaaaa tacgtcacgg
     1621 ctccgccaaa cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnngctttg atttctgtaa gcggattttg aatgatagac tttcatggaa aactgtgctg
     1801 caaaatcatc ccattcatca tccggaatat caatgtagat cgcatcgggt gtcccgttct
     1861 gatccgtcac ggtttcctga agcgtgttaa aataataaac tccgttctta tacaggaaaa
     1921 acgtaagaca tccgcacaat accgtgatca gcactgtagt aaggatcgtc agtctccact
     1981 ggagtgatat ttttctcatg ttttgtcatt tcctcctatt ttataaccct ctccgatctt
     2041 atttacgacc ggatcgtaac caagttctgc cttcagtttt ttccggagag aagacatatg
     2101 gacccggatc gaaccactga aactgtccac agaagaatcc cagacatgtt cgatcagttc
     2161 ttcctgactg accggccgcc cctggttcag aagaagatat tccaggatcc cattctcttt
     2221 tcttgtcagt gcaacagggg tctcttttgc atacgctgtt cgttctcttg tgttgaaacg
     2281 aagagcaccg cattccagac agacattttt ctgtacaaat tttcttctgg tcagacttct
     2341 gacacgagct tccagttcca gcagatgaaa tggtttttcc atatagtcat ttgctccggc
     2401 atccagccct tcaactttat ctgcgatctg acttcttgca gaaaggatca gtacctttgt
     2461 ctcttcattt tctctgcgaa gttctctgag gatatccatt ccgtccattc ccggaaggtt
     2521 aaggtcaagt acgatcagat cgtacatttc cgcacagatc atctcaagtg cctcttctcc
     2581 gtcattgcac gtatccactt cataaccggc ctgatgcagg ctttttgcaa tcatatcaca
     2641 taattttttt tcatcttcaa caactaataa tctcaatttt ttctctcttt tcttaacagg
     2701 aattttacac ctgttattgt ataatgcaat catcgaaagg tcaataaaag gactgtaaat
     2761 aaggaaagga gtgtcttgat gaaatcagaa aaagcaacac agattctttt gcttgcagca
     2821 ggtatgctgc tttttcttgc cggaattttc cgcggggaat ctgcagttgt acttagtaag
     2881 gcaataaaaa tatgtatgga gtgtgtcgga attggataaa aaaataaaat cagccaaaaa
     2941 cattccagcc cgcttccgcg gatggatcca ggcagctgca gtgctgctta caaacattca
     3001 catcccgaat ctgtttaaag ggaaaattta tcagggaaaa gcaaagacaa tgtgtgtgcc
     3061 ggggctgaac tgttactctt gtccggccgc cacaggtgcc tgtccgatcg gagcttttca
     3121 ggctgtagtc ggttcttcaa agtttaagtt cacctattac atcacaggct tttttatctt
     3181 gctgggagtt ctgctgggac ggtttatctg tggttttctc tgcccgttcg gatggtttca
     3241 ggatctgctg cataagattc cgggcaaaaa gttttccact gcaaagatga aacctctgcg
     3301 gtatctgaaa tacgtgatcc tggttgtctt tgttatactg cttccggcat ttgtaacaaa
     3361 ttcacttggc atgggagacc catttttctg taagtacata tgcccgcagg gcgtccttga
     3421 aggtgcactt ccgctcgccc ttgcaaattc aggaatccgt tctgcacttg gacatctttt
     3481 tacattcaag tttacgatcc ttgtgctttt cattgtcctg agtattctgt tttaccgtcc
     3541 gttctgtaaa tggatctgtc cgcttggtgc catctactca ctgttcaaca gagtatcctt
     3601 tcttaaaatc caggtggatc acaaaaaatg cataggctgc cggaaatgca tccgggcctg
     3661 caaaatggac gttaaagtaa cagaaacacc aaaccatccg gagtgtatcc gctgcggaga
     3721 atgtatgaaa gcctgtccgg tggatgcagt atgctatcat tacggatttt caaagaaaaa
     3781 acaggctgac gatcaacaag aaaaatcaaa tggaaaagga gatttaaaat catgaaaaac
     3841 aaattcaaaa aagtaatcgc tgcatcactt gcagtatccg tacttcttgc ttttacaggc
     3901 tgtggcagca atcaggctcc gtcagcagac aataaaaaac aggaatctgc ttccacggca
     3961 gataccgcag acatggatga acaaagcaaa aaaagagatg acctttatca gcaggaaaat
     4021 cagatttttg ctgaccacaa agatgcctgg gacaaggtat ttggtcttat gaacaagaac
     4081 agtgccggtg agagcctgag tgaaaactat gcagatgtcc ttgcaggcat cgttgaatct
     4141 aataaagatt ctttcacgga agaagaatat gatacgctgt gcaaagacat tgagacgatc
     4201 cgcggcattg aagaagagat cgcagcgatc gaaaaagaga ttgcagaagc ggattctgcc
     4261 aacggtggat cctcagaatc agatgaatct attagtttat tccaggattt cacgggaaaa
     4321 gacctggatg gaaatgatgt cgatgaaagc tttttcaaaa ataacaaagt taccgttgta
     4381 aacttctggt tcagcggttg taagccatgc gttgaagaac tttccaaact gaatgaatta
     4441 aatgaaacac ttaagacaat gggcggagaa gttgtcggta tcaatacaga cacgttagat
     4501 aacaatcagg acgggatcaa agaagcacag gacattttaa aagcacaggg tgcttcttac
     4561 aaaaacctga cggttgcttc agattcagca attggaaaat atgccagcag cattatggca
     4621 tttcctacaa cagttcttgt agataaagac ggaaatatcg taggcgagcc tctgatggga
     4681 ggaatcgatg atcaggcaaa ttacgatcag ctgatggaac agatccaggc tgtcatcaat
     4741 cagtaaatgt caaggggggg ggagaccccc cttttttttt gcgttcgcat acgttttgaa
     4801 acatgaaaaa cagaaaacaa ttttgtgcaa aatatataag aatttaaaac aaaaaataaa
     4861 aaagtggtgg taaatcgtat aaaattacat agaaaaaata aatcaaacat ggtaaaatgt
     4921 ataaacattg agaacgaaaa tcggacagaa aggcactgtc gagtacaaaa gtaagaagga
     4981 gggcagagaa aagaattgcc tatggaaagc atgaatctga ttgagtccag aaggagcgaa
     5041 attctggcag aagggagaga aatcatatga agatgagatt cagaaagatt atggcagcca
     5101 ttctttcgac agcaatggtt gctgctgcga taccggcatc cggtattctg aatgtacagg
     5161 cgaaatcgcc gcaggagcat attgatctgt ccagacaggt ggcaggtgaa ggtatggttc
     5221 tgatggaaaa tgatggtgca ctgccattgg cacagggcga aaaagttgcc atgtttgggc
     5281 gtgcaatgat tgattatgta cgaggcggcg gaggaagcgg tgcaacgaac gtggattata
     5341 cgcacaacat tctccagggg atgcagatca aggaagaaga aggaaaggtc accctctatc
     5401 agccgctggt agatttttat acgaaacagg tcagtgaaaa aggcattaaa aacgatgcaa
     5461 atattacggt taccgatgat atgttaaatg ctgcggcaga aaatgcagac accgcaattg
     5521 tgacgatcgg acgttattct tcggagggaa gcgaccgcag tgcatcaaaa ggtgactact
     5581 atctgtcaga tgcagagaca gagcttctga cacgagtggc agcgaaattt aaaaagaccg
     5641 ttgttgtatt gaatgtcggt gccgttctgg atacggaatg gatcaaagac attccgggta
     5701 tcgattctgt gctgatggca tggcaggcag gtatggaagg tggacttgcc acagcagacg
     5761 tgctggttgg agatgtaaac ccatccggta aatttgtgga tacatttgcg aaagattata
     5821 aggattatcc aagttccgat acattttatg aatcaaattc ttatgtaaat tatgaagaag
     5881 atgtgtttgt cggatatcgt ttttttgaaa cttttgatcc agagtatacg aaagtgaatt
     5941 acgattttgg atatggatta tcctacacaa cttttaaaac agacaatgta aacgtcaaag
     6001 tagacggaga aaatatggtt acgacagcga ctgttacgaa taccggaaag gtagccggca
     6061 aacaggtcgt gcaggtttat ttctcagccc cgcagggaga gctgggcaaa cctggaaaag
     6121 agctggcagc gtttgcaaag acaaaactgc tgcagccggg cgaatcgcag gagctgacaa
     6181 tgacgtatgc gatcagtgat atgtcaagtt acgatgacac cggaaaagtt cagaaatccg
     6241 catacattat ggaagccgga gattacaaga tctacgtcgg caattctatc aaagatgccg
     6301 gagaacatgg cgtacgttat acctataaag tagaagatac tgaagttacc gagcagcttt
     6361 cacagcagtg tgcaccggtt cagttaaaga agagacttct ggcagacggc acatacgaag
     6421 atctcaagac gaattcagat gaaatgaatc cgcattacac ggttccggca gaaggaacga
     6481 caaaagtaga agcagagaat ttcctgaatt cttccggaaa cctgagtgtg gaaacatttt
     6541 atgatgataa tctgcagatg aaaaaatgtc tggcattttt aaatgaagca ggcagataca
     6601 tggaatatga gctgaccgca gaagaggccg gcaaatatga agtgatcatg tgtatggcaa
     6661 atggctatcc agagatccaa aactgtttta ccgttgcagt aaacggtcag cagcagccgg
     6721 gtatttcatt taatgcacag cagaccggtg atggaaatgg tgcttccgag tggtataatt
     6781 ttgaagaatg tgaaccattt tacatcaatc ttgacaaggg cgtgaacctg gttcgtttca
     6841 cggcaaataa aaataatcca aactatgatt atatactgtt cagacgtgtt ggcgatcagg
     6901 aggaactgta caataaagtc atcaaagcag aaggtgcgaa caagattgag gcagaaaaat
     6961 acgatgcagc cggtacgaca ggcaacaacg cagtacgcac cgaaaacttt acaaaagatg
     7021 attacacagg gacttgcctg gcttatatga attatgccgg aaactatgtg agctactatc
     7081 tgaatgttga agaagccgga gattataatg tgatattaaa tgtggcaaat ggccgtgcgg
     7141 cattcgattt tgatccaggt atggaagtag acggaacgaa attcccggca acgatcaatg
     7201 caaaacagac aggtgacggc gaaaagaatg aatggtacaa tttcgaaaat ctggaaccgg
     7261 ttaccgtaac ccttccgaaa ggacacagca tcctcacact gagggcatcc gaaaaggata
     7321 aatacccgaa tatcgattat attcttgtag aaaagaaaca gacagcaaca cagagcctga
     7381 agagtgcggc agcaaaaaaa gcccgtacag ccgaagatac accagcttct gaaccggcag
     7441 acagcggcaa gatcatgctg gtggatgttt ataataatcc ggaacttatg gatgatttcc
     7501 tggcacagtt aagtgatgac cagctctgcg gactgatggg tggtcagaca aataccggtg
     7561 ttgcaaatac cggcggtatg ggaaatctga tggaatacgg tattccaaat gcgatgacag
     7621 cagatggtcc gcagggtatc cgtatcggta caacctgtac tgcatggccg atctccacac
     7681 ttcttgcaag tacctgggat gtagatctgg tgaagcaggt aggaaaagcg gcagcagtgg
     7741 aagcacacaa caatggtatt gatatctggc ttgcacctgg tatgaatatc cacagagatc
     7801 cgctttgcgg acgtaacttt gagtactatt ccgaagatcc tctgattacc ggaaaaatgg
     7861 ctgctgcaat cacagaaggc tgccagtcaa aaggcgtttc cattacactg aagcatttta
     7921 caacaaacaa caaagagaca aaccgaaaca gcagcgattc cagagtcagt gaacgtgcac
     7981 tgcgtgaaat ctacctgaaa ggttttgaaa ttgcagtcaa agaagcacag ccttggagca
     8041 ttatgacttc ttataacttc ttaaacggaa tcgaaacatc tgaaaataaa gatctcctga
     8101 caaacatcac ccgcggtgaa tggggatatg aaggaatctt tatgacagac tggggcaaca
     8161 actccaatca tgcaagagaa gtcctggcgg gaaatgatgt caaaatgcca agcggttctc
     8221 cggcaacttt aaaagcggcc ctgaagaaag gcatcctgaa acgttctgat ctggaagact
     8281 gtgcagaacg tcttgtgaag atgatcatga aagtgaacat ctttaaagaa aagatcctca
     8341 atccggtgac agtagacatc ggtgatgaca catactttaa agcagcagaa aatattctct
     8401 ggtcacagac ggcaaggggc gaaaatacat ctgacgaaga tggcggaaaa gacctcggtt
     8461 actgtgatgc aggagcatgg acacagtatc agatcaacgt tgccaagagc ggaacttaca
     8521 gcctgtctgc acgttctgca tccaatgcag gcggcggagc atttgatatt ctggcagacg
     8581 gcacaaagat tgcaagtttc aaggcagtca acaccggcgg atggcagaaa tggaccacac
     8641 tggaagcaca gcagatcaaa ctggaagcag gagttcatac actccgtatc gagttcacag
     8701 agagcggttc caacctgaac tggctgcatt ttgtaagaca gtctgaatac attgaaaatc
     8761 tggaaaaact gtaccaggca tgggcaagtc aggatctttc tgaatacaca gcagaaagtg
     8821 cagaaaccat gagaacagcc cttgcagcag caaaagcagt tctggataaa gaaaatgcaa
     8881 cggattctga gatccagaca gccatcattc agatgacaca ggcgatcaat gctatggaat
     8941 acggcacaaa caaggtacat ctggaagcga tcattgaagt agcagaaaag actctggcaa
     9001 atccgggaga ttatgcagat gtttccgcac tggaagcagc agtaaaagcc ggaaaagaaa
     9061 ttctggcaaa agcagatgca actcaggaag aagcaaatgc agcagcaaat gcgatcgcag
     9121 acagcatgca ggcactgatg gaaagagcag atatccgttc cttaagagaa ctgacagatg
     9181 cagcaaacga gcttctggaa aagaacagcg gcaagtatac cgatgacact gcaaaagcat
     9241 tgcaggatgc agttgcccat gcagaagaaa tcctgggtaa agacggaaaa tccgatgtag
     9301 agatcaatga agcttacaat cagatcatgg atgctattgt cggacttaag atgaaaggca
     9361 acaaggcagc tctggaagca atgatcaaaa aagcagaaag cattctggcg gaaaacagca
     9421 gatatgtagc ggctacgatc gaaggtctgg atgaagaact tgcagcagca aaagcagttt
     9481 atgaaaacaa tcaggcactg cagagtgaag tcaatgaagc tgttaagagc ctcactttca
     9541 agattgcaga agcaagactt ctgggtgatg tagacggtga cggtcagatc acaacaaatg
     9601 ataccgtgga aatcctgcgt gcggcagctg agattgccac actttctgca gaagatgcag
     9661 caagtgcaga tgtaaacggt gatggtgcgg cagataccgc tgatgcagtg ctggttcttc
     9721 agtacgcatc cgaagcagtc agtggattct gattttttga acgcagagaa gcattccccc
     9781 gcttcaagtg tgcagagcat taagcggtgg gtgaggggga tgcttatgtc agtgggagtt
     9841 gaaagctccc actgacaggt cgcgaagcga ctgcgttcat gagcaagtag cgaagcggat
     9901 tgcgaatgtc cgctttacgc aatgagcgat tctgaaaata aaaaagcagc gggagtctga
     9961 taaactcccg ctgtttttgt ttgactgcca aaaaatcaga gcgtataatg aaaaagtaac
    10021 caggtcgatg aatacaatat tctaaaggag aagaacaatt atgaaaagtg cagcaaaaac
    10081 cccgggcaag ttgcttgcag gtttgtgcct gatactgttt ttctgtctga tgccggtccg
    10141 gtcttatgca gcagagcagc agctttctga gatcacaaaa ctgtatgtaa cgggaattta
    10201 tggcagcgaa acactgccgc agcagaaagt caactggcag tatgcagata aggggtatta
    10261 tctgtgtatg ccttccggag cagatttaag caatgtacag cttcatttca cagaaaacag
    10321 tgacggaaat gaaaatgccg acagttatgt tatgatcggc ggacaaaaaa taaactgcgg
    10381 agattctaca gatctctcag gaacaagcaa attgcagatt tcactcgccg gaggcaaaag
    10441 tgttagcgta aatattgtaa aatcggcaga gattccggca atgtttatcc agacagcatc
    10501 cggtacgctc gataaggttc atgcnnnnnn nnnntggcag aagagaccgg gattccattc
    10561 accatggaca gccgaaacat cgatctttat atcaatggcg attacatggg atcttatctt
    10621 atcacagaaa aaatcgaaat cggaaaaacg agagtcaaca ttacggatct ggaagaggcc
    10681 acaagtaagg caaatgataa tgcagatctg gaaacatatg agcagaaagg aacaaatgat
    10741 tataaagcag gcacacagaa atgggtagat attccaaacg atccggaaga tatcacgggc
    10801 ggatatctgc ttgaacttga gcttggagag cgttacaaag atgaaacaag tggttttgtg
    10861 acaacgggcg gacaggcagt cactatgaaa tgcccggaat gtgtatcgga aaatcagatc
    10921 aaatacatca gtgaatttta tcagaatatg gaaaatgctc tttattccaa agatggctat
    10981 acaacggaca gcaaagggga gcgtcatgct ctttctgatt atattgatat cgaatcactg
    11041 gcacgtatgt acctgctcca ggaattttcc atgaacctgg attccggtat cacaagcttt
    11101 tatctctata aagactctga tctgaccggt gatggaaagc ttcatgccgc accggtatgg
    11161 gattttgatg tggcacttgg aaattacacc tcccgaaatg gcacagactt tactgacccg
    11221 acacagtggt gggcaaaaat ttccagaatg tacgacaaca gcagtaaata taatgtcatg
    11281 gcacaggctg tgcagcacga agaagtgtgg aataaagtaa aagaattatg gcagagtgaa
    11341 ttcatgccgg cgatcaagta catactggga gagtccaccg cctacacagc aacaaagata
    11401 aaaacactgg atgcatacaa agcagaagta agtgcatccg cagcgatgaa ctttatccag
    11461 tggcgtgacc tgatggagaa tccatggaat cacagcagtg aaagttttgt aaataccggt
    11521 cttacttatg atgataatat tgaatatctg agaaatttta tgacgaaaag atgcgatttc
    11581 ctgaaccgta acctgggcgg ggaaagtaca ggaacagatc cggatacatc gcagaaagta
    11641 accgtaatag aagatgatac cagattttat gcagcaaaaa atattttaaa ttcctccgga
    11701 gatgtcaggg cagaagatac atcagatgaa ggcggaggag agaaccttgg ctattgcaac
    11761 cagggcagtt taacagaata taaaatcaat gtaacgacag ccggtactta taacgttacg
    11821 gcgcgtgttg cttcgaatga cggaacagga gcattcagca tttatgtcga taatcagaaa
    11881 attgcaacat accgtgcagt caatacaggc ggatggcagg tttggacaac tacagatgcc
    11941 caggagatca cactggaagc cggagaacat tcatttgcca tctactttga agaaagcggc
    12001 atgaatctga actggctgca gctcacacgg gaaacgggta ccgatccgaa accagacccg
    12061 aaaccagatc cagacccgac tccagatcca aatccgactc cagatccaaa tccgactcca
    12121 acgccggatc cagacccgaa accagatcca aatccgactc caacgccgac tccagatccg
    12181 accccaacac cggatccgac cccgactcca acaccaagtc cgaaaccgga ttcaagcaaa
    12241 gatcaaaata cggtaacgct gacaaagggc agtatctgtc aggacgcaaa aggtatcctg
    12301 aaatatcgga tcacaaaaat ggcagccaaa aacagcaccg cagaagtgat cggaattcag
    12361 aaaaaatcag gaaaagtaac aattccgtcc acaattacgg ttcagggcat tacctttaaa
    12421 gtaacagcga tcgcagagaa agcttttcga aatgacaaaa atctgaaaag tgttgtcatc
    12481 ggatccaatg tgaaaaaaat cggaaaacag gcatttgaaa aatgtaaaaa actttcttct
    12541 gttacattca aaggaaagaa agcaccgacc attggaaaag cagcctttaa agggatcaag
    12601 aaaaaagcaa atgttcaggt tgcgggcagt atgaaaaaaa atcaggtgaa aaaattacag
    12661 aatcgcatga aaacagcagc accttctgta aagatcactt acaagaaaaa aataaccgtt
    12721 cgtttttagc ggaacacagt aaaaagcatg ccatagcata cagaaaaaca gtttctgaat
    12781 gttatggctg tttttctttt gtgaaaagta tgatagtata gtgatggaac aggcagataa
    12841 attacaggag gaaccgacaa tgaaattatg gacaatccag ctctgacatc tgttaatcca
    12901 ttgttgatcg atctcaacga ttttcagttc cttatgccgc cgattcagta ttttccgccg
    12961 tatacagaaa aatcggtaga ccgcatatgt acggatctgg aaaaaggaca gatccgccag
    13021 tctgaatttc caagctatgt aattcaggca catattccat atattgcacc gggagatctg
    13081 gtaaacgtgt atccgttgtt tgaaataagg ctaccgcaat cccactggct ttagacggtg
    13141 ggttaaggta gcc
//
LOCUS       DAWDWH010000033        12981 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_2622_length_12981_cov_13.429212, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000033 DAWDWH010000000
VERSION     DAWDWH010000033.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 12981)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12981)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12981
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2622_length_12981_cov_13.429212"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    772..781
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3135 a         3338 c         3000 g         3498 t
ORIGIN      
        1 cgttgcccac aagaagagca ttccaacggg aaccaggatc ttcaggcgta tttcgcttac
       61 ggctctgaag atttttctgg tacttctgct gttttgcgtc atccttggca cggcggccgg
      121 tgtcggcatg atcaaagggc tgatcgccag tgcccctaac atcgattctc tctcggttgc
      181 accggcagaa tcggccacct acatctataa caccggcaat aaaccggtcc agaagcttgc
      241 cgaatcaact tccaaccgta ttcttgtgaa gctggatcag atcccggagg atctgcagca
      301 tgcgatcgtt gccgtcgagg atgagcgttt ttacaagcat cacggcatcg atatccaggg
      361 tatcgcccgt gccgcagtga tcggcatcac cagtggggat ttctcagagg gtgccagtac
      421 gatcacgcag cagctgatca agaataatgt ttttacggac tgggtgacgc agacggctct
      481 ttctcagaag ctgcgtcgta agttccagga gcagtatctg gctctgcagc tggagaaaaa
      541 gatgtcgaag gaccagattt tgcaggatta tctgaacacg atcaacttag gtgccagctc
      601 gtacggcgta caggctgctg ccaaacgcta ttttaataaa gatgtatctc agctgaatct
      661 ctcggaatct acggtcattg ccggcattac acagaatccg acgctgtaca atccgatcat
      721 caatccggat gagaatgcaa agcgacgcaa gatcattctt cagaaaatgg tnnnnnnnnn
      781 nagcgaacag cagcagcagg atgcccttgc ggacgatgtg tattcccgta tccagaaaac
      841 ggctcaggag acggacgagg tttctattta cagttactat acggacgctc tgatcgaaca
      901 ggtcatggat gaccttgtgg aagtcaaggg atacaccaga cagcaggctt acaaagctgt
      961 ttactccggc ggtctgaaga tttattccaa tcaggatgaa gagattcaaa aaatctgcga
     1021 tgaggagttt gccaacccgg agaattatcc ttcggtgacg ctgattggtc tcgattatgc
     1081 tctgagtatc cagaaatccg acggcgagat tatcaattac agcagtgaaa tgctccgcag
     1141 ctggttccag aaacaggaca gctcttttaa catgatgttt gacgatgagg aatctgcaaa
     1201 agctgccgca gagacttaca aaaacgccat ggttaaaaaa ggcgatactg tgcttggcga
     1261 gcgtgtttct cttgttccgc agccacaggc ctctgtcgtg atcatcgaca atgagaccgg
     1321 atatgtgaag gcgatcgtcg gcggacgtgg cgaaaaggaa gccagcctga cgctgaaccg
     1381 tgccacggaa acccgcagac agcctggttc gacttttaag atcgtcagca catacgctcc
     1441 ggctcttgat gccgagaata tgacgcttgc tacggtattc gacaatgcac cttacaatta
     1501 cacaaacggt gtcccggtca ataactgggc aggtaaaaat gcctacaccg gactgactac
     1561 gatccgtcag gcgatcgcag gttcaattaa cgtagtagca gtcaaatgtc tgaccgagat
     1621 cacaccgcgg cttggttttg agtatgccga agctctcggc atcagcacac tttacgatga
     1681 tgaaaatctg gacgtacgcc agccgctggc tcttggcggt atcacggacg gtgtcgtaaa
     1741 catcgagctg tgcgatgcct atgcaacgat tgctaacggc ggcaaataca acgaagcgaa
     1801 gttttattcc cgtatcgagg attccgaagg aaaagtgatc attgacaaca ctccggaaga
     1861 aaagacggtt ttaaaggaca gcaccgcttt cctgctgaca caggcgatga tggatgttgt
     1921 aaaagccggc ggaacagcct ccgatgtcag cttcggcgag atgccgatcg ccggcaagac
     1981 cggtacgact tcggacgacc gtgatatctg gtttgccgca tacagcccgt attatacgtg
     2041 tacggtctgg ggcggctatg acaaccacga tacgctcccg tccggcgatc tttaccacac
     2101 gtatcacaaa aaactgtgga ctgcgatcat gagccgcatc cacgaaaatc ttccggtcag
     2161 acagtttgaa cagccggaca gtgtggaaac tgcctatgta tgtaagaaat ccggactgct
     2221 cgccgtggat ggtgtctgca ccggagatcc gcgaggctcc atggcttaca cggaatattt
     2281 tgcaaaaggc acgacaccga ccaagagctg cgatgtgcac acggctgtaa aggtctgctc
     2341 ttccaccgga ctgctcccga ctgcgacctg taccacgaca acgaaaatct ttgtcaaacg
     2401 accggccgga agcgagggca caacggaaga ttccgcttat gctccgccaa gtgcgacctg
     2461 caaaggtcac aacatcctgg ataagatcac agatatcctg aatccgaaaa aagatgcggc
     2521 agatatggat gccgatgatt cgaagaagga tggtgcgacc acgccgggtt ctgatgcaaa
     2581 ttccggttca acaactacac ctgacagcaa ttccggctcc ggcgattctt ccggaagcgg
     2641 aggtaccggc tctggcacag gttccggttc cggcggcagc acttccggcg gtggctctgg
     2701 cggagactcc ggaggcacca cggctccggg cggagatgag atcctggaga tcattccata
     2761 aaagcatctg ctctgttgtt caaagcattt gttgtctgaa tcttaaaatt tcatttggat
     2821 acaaaaaatt ccatgaggat attacttaaa tcaagtaaat tctcatggaa tttttattta
     2881 tctcagtcgg agaagactct cacttctgca agtactgcgg tgctgactgc cgttgtttta
     2941 ttacagcatc agttttgctg caccgtagat accggcatca ttgcccagtg ttgccagtgc
     3001 aaattttgca tctttgcagg atggaaatgc atattttttg taatatttct cgatataatc
     3061 aaagagtatc tttccggctt tggagacgcc gccgccgatt acaaatacct gcggatctac
     3121 cacacctgcg atgtttgcac atgcaagacc gaggtatctg ccgaattctt ctgcgatttc
     3181 ctgtgcaact gcatcgcctg cttttacggc atcaaagacg ctttttgcac tgacttctcc
     3241 gtcacgcaga acggaagggg tatcgcagga tgccagcttt ttgtttgcca gatatacgac
     3301 acctgttgca gatgcaacct gttccagaca tccggtgttg ccacagttgc aaggtttgtc
     3361 aacgctgtcg gttacatgga tatgtccgat ctcaccgccg gctccgttgc ttccggttac
     3421 gatcttgcca ttgatgatga tgccgccgcc gacaccggtt ccaagtgtca ccataacaac
     3481 attgtgatag ccgactgctc cgcctttcca catttcgccg agtgctgcca cgtttgcatc
     3541 gtttccgacg cgtaccgcaa ggcctgtctt ttcggaaagc tcttttgaaa gtggtacaaa
     3601 tccccagtgc agattgacgg ctaccggaac gttgccttct tcatctgccg gtcccggtac
     3661 accaacgcct gctccgacga tctcttcttt gtccagacct ttttctgcga tcttggcatt
     3721 catcgtctct gcgatgtctg cgatgatatt ttcgccgttg ttttcggttc ttgtcttaat
     3781 ttcccattta tccaggactt ctccgtctac attaaaaagt ccgcatttta cggttgttcc
     3841 gccgatatcg attccaaaac aatatttttt catttcttct gctcctcttt tttctttgca
     3901 agtgacttct gaacacggtt ataaagctcc tgagctgcat tgtatcccat ctttttctgt
     3961 ctgtggttaa cagcagcaga ctctacgatc acagccagac ttcgtcccgg acggatcgga
     4021 atggaatggc atacaacgcg gttgccgagg aactcggtgt attcttcctg aagtccaagt
     4081 ctgtcgtatt ctttgtcctt gtcccactct tctaatttga taaccaggtc aatggtctgt
     4141 gtatttttca cgctctccac accaaataag gtcttggcat caatgatacc gataccgcga
     4201 agctcgataa agtggcgggt gatatccgga gcacttccga ccaagctcgc atcgctgact
     4261 ttgcggatct ctacgacgtc atcggatacc agacgatggc ctctcttgat cagctccaga
     4321 gctgcctcac tcttaccgat gccgctctcg cccatgatca ggacaccttc gccgtataca
     4381 tctaccagca cgccgtggat agagatacaa ggagccagct ctacattcag ccagcggatg
     4441 atctccgcca taaacggaga tgtatttttc tctgtggtaa agaccggaat accgttctct
     4501 gtggcaatac gcaggatatc ttcatccgga tccgttttcg atgtaaagat aatgcacgga
     4561 accttatggg cacagaactg ctcataggca tgtactctct gatttctcgt catatgctcc
     4621 agataagtaa attctacata accgacaatc tgtacacgct cggatgcgaa atgttcgaaa
     4681 tatccggtca gctgcagtgc cggtctgttg atatccggtg tggtcaccac gatcgatgaa
     4741 atatcaacat ccggtgtcag attcttcagt tccatgcttt ttgcaatttt tgtcaattct
     4801 acactactgt tactgttcat ctgtttttgt cctttcctgt cagtaaagcg gacattcaca
     4861 atccgcttcg ctacttgctc atgaacgcac acaacatcgt caaatccatg cacgttcctt
     4921 cgatttcatg ctctcattct atcacatccg tacacatcat acaagaaaca aaacagattc
     4981 tgtaaaaaat tgtatactgt tcacatgaaa attcaagaat gccagaggca ttcttgctgc
     5041 atacaatcat cctttttctt cttttttctt ttcatggaag aaagcgtaga cttttcctgc
     5101 acttaaggcg gtcattgaag gcactgctgc cagttcctct tcgctcgcct cgcggatcgc
     5161 ttccagggag aggaaatgac gcatcagtgc ctttcttctt gccggaccga taccgtcaat
     5221 gtcatccagg atcgaatgta cctgatcttt gcttcgcagg gaacggtgat actcgatggc
     5281 aaaacggtgg gcttcatcct gcacgcgggt gatcagttta aatccctcgc tgtcgcggtc
     5341 gatcgggatc tctacgttat tataatacag gcctctggtg cggtgccggt catcttttac
     5401 cataccacag accgggatgt taagatccag cttttccaga acgccaagtg cgatattgac
     5461 ctgaccgcgg ccgccgtcca tcatgatcag atccggcagt tttccgaaac tgtcatactc
     5521 ttttttctca gggtcactct cgtgtgtaaa acgtcttgtc aggacttctt ccatgcttgc
     5581 gtagtcgttt ggcccctgca cggttttgat acggaatttg cggtaatcac ttcgttttgg
     5641 tttgccgcgt tcaaagactg ccatggatcc gaccgactcg aaaccgctga tattggaaat
     5701 atcataggct tccactcgta ccagatctgg cagatccagc caaccggcaa tttctttcat
     5761 cgccccgatt gtgcgtcctt cttctcgttt gatacgatct ttgtcctgtc gcaggacaat
     5821 ctctgcattc tgcgtggcaa gctcaaccaa tttttccctg gtaccttttt tcggtacttt
     5881 gatgtgcacc ttctgccctt ttcgctttcc gagccactct tctaagacgg actgatcttc
     5941 gatctcacac gggatcatga tctcccttgg aataaacggc gtgccggaat agaactgctt
     6001 cataaaactg gaaatcacga tattctgcga atcgtgcggt gcgatccgca ggaagaaatg
     6061 gtctctgccg atcagcctgc cctgtctgat aaaaaatacc tgggctactg cgtcgctgcc
     6121 gtccattttc acggcgatca catctttgtc ctcgccgtcg ctgcttgtga tcttctgctt
     6181 ctgtgcgatc tggctgacgc tgtttttgag gtcacgccaa gtcgctgcct cctcgtactc
     6241 catacgctcc gaggcatcca gcatccgctt ttcgagcatt ttcaggattg gtccgtagtt
     6301 tccattcaga aaatccatcg ctccgtttaa ggatctgcgg tactcttcct gactgatata
     6361 gccctgacat ggtgcatcac actgtttgat gtgataattc agacaggggc gttcttttcc
     6421 gatatcctgc ggcagtctgc gattacaggt acggactttg tagatcttac ggatcagctc
     6481 gatcgtatct ttgactgccc cggcactggt gtacggtccg taatatttat ttttatcctt
     6541 tttcatgctc cttgaaaaca aaatccgagg atatgcctcc gatgtcgtta cttttataaa
     6601 tggataagtc ttatcatcct tgagcatcgt attgtacttc gggcggtgtt ccttgatcag
     6661 attgcactcc agaaccagtg cctccagctc ggaatccgtc acgatatact caaatctggc
     6721 gatccttgtg accatctgct cgattttggc acccttattg cggctggtct gaaaatactg
     6781 ccgcaccctg ttcttcaggc tgatcgcctt gcccacataa ataatggcat ctctgtcatc
     6841 atgcataata taaactcccg gcttagccgg gagctttttt aattcttcct gaatatcaaa
     6901 catctgcttt tacatcctca cttactcggt tgcggtgtct gctgtgttct cttttgaaag
     6961 atttactttt gtgctgtttt cttcggtctg tatttcgctg tttttctgtg cttcttctgc
     7021 ctgtacttta cgcaggatct ccataaattc ctttccggtg atcgtctctt tctcgatcag
     7081 gaaagcggcg atgtgatcca gaatgctgcg gtttgccgta agcatgtctt ttgcttcctt
     7141 atatgccttt ttgagcatgg cagtgatctc tttatcgacc tcggcctgtg tcacctcact
     7201 gcagtccaga accgtctgat tatcgaggta ctggtttgcc gcacgctcca gaccggtcag
     7261 tccgaatttc tcagacatac cgtagcgtgt gatcattgag cgggcaatgg aagttgcttt
     7321 ctcgatatca ttggatgctc cggttgtcac tgaaccaaat acgatttcct ctgctgcacg
     7381 gccgccggta aattctacca gtctcgccca cagttcttct tttgtataga ggcatttctc
     7441 ctcttccgga atgttcatga catatccgag tgctccggat gtacgcggca cgatcgtgat
     7501 cttctgtact ggctctgcat ctttttgcag tgcgctgacc agtgcgtgtc cgacttcatg
     7561 ataggaaacg atctcccgct cttttttgct caggatcttg tttttctttt cttttcccat
     7621 ctcaaccagc tcgactgctt ccgataaatc tgcctgagat acggcacttc ttcctgcttt
     7681 tacagcaagg atggctgctt cattgaccat atttgctagg tctgatccga cagctccggc
     7741 tgttgccagg gcgatttctt tgaaattgac cgtctcatca agattaacgt tctttgcgtg
     7801 tacacgcaga atatcttctc ttcctttgag atccgggcgg tctacgatca ctcggcggtc
     7861 aaaacgtccc ggacgcagaa gtgcagagtc cagaacttcc ggacggttcg ttgctgcaag
     7921 gatcagcaca cctttggagg aatcaaaacc atccatctct gcaagcagct ggttcagtgt
     7981 ctgctcacgc tcgtcgtttc cgccgccata acggctgtca cggctcttac cgatcgcatc
     8041 gatctcatcg ataaaaatga tacacggtgc actctttttg gcctcttcaa acaggtctcg
     8101 cacacgcgat gcaccgacac cgacaaacat ttctacgaaa tcggaaccgg aaatggagaa
     8161 gaacggcacg tgtgcctctc ctgcgactgc ctttgccagc agtgtcttac cggtacccgg
     8221 agggccgaca agcagtgctc ctttcggcag cttcgctccg atcttcacgt atttctgcgg
     8281 atcttcgaga aatcctacga cttcctgaag cgattctttt gcttcatcct gtccggcaac
     8341 atcctggaag gtcacaccgg tttccttctc gccgtaaact tttgccttgc tcttgccgac
     8401 acccatgact ccgccgcctg atcccatacg acgcatgaaa aagtagaaaa taaatccgat
     8461 cataaccagc ggcagaacat ttgccacgat atttaagatc aggtaagaag aattgtccgg
     8521 tacggttttg gtacactgta cgcctgcctt gagcagtcgg tctgtcagtg tcggatcgtc
     8581 catgatacct gtataacagg tgatcttcac acccggtatt ggctgatttt tcaggttgat
     8641 gtcgattcga ttggaaccga tctctacaga ttcgacctct ccgcggtcaa ccatgttaat
     8701 aaactgatta taagtgatct cacgctcggt tgtatctttt accatgctgg taacgatatt
     8761 cgtggcaagc atcacaatca aagtaatcac cagaaaaacc agaatgcttt tgccgccgcc
     8821 ttgctttttc ccattgttct tattgcgacc attgtccggc tgtttttgtc cacccggtcc
     8881 ctgtggtctg ccaggaccgc cgccctgcat ctgattgttg tccatttttg tcctcctcat
     8941 atgcaaactc caacttatcc cattataagt acagtttcag aagaaatcaa tatatagatt
     9001 gtgaaaaaaa gctcacaatt atgaatcttt tctaaaaaaa cggtagccga acgggtatga
     9061 tttattgtat actagtgcaa gagttacttt gtaacaagga aatgcacgcc ggttcttgcg
     9121 tgcatccgac ttgaattttt aggaaaggtt cttaatcata tgaaaaaagg atttgacaac
     9181 gaaaaatatc ttgctacaca gtctcgctgt atccgcgagc gtattgacaa attcggagac
     9241 aaactttatc tggaatttgg cggaaagtta tttgatgact accacgcttc ccgtgtactg
     9301 ccgggatttg agccggattc caagcttcag cttctgatgc agctgtccga gcacgcagag
     9361 atcgtcatcg tcatcagtgc ggtggatatt gaaaaaaata aaatccgcgg cgacctcggc
     9421 atcacttacg ataccgatgt actgcgtctg atcgactctt tcgaagaacg tggacttttt
     9481 gtcggaagtg tcgttatcac aaaatactcc ggacagacca gtgcagacct gtttaaacaa
     9541 cgtctgatca aactcggcat caaggtttac accctctata tgattccggg ttatccgaat
     9601 aatgtctctc atatcgtaag tgatgaggga ttcggtaaaa atgactacat cgaaacttcc
     9661 cgccctctgg tcgtgatcac tgcccctggt cctggaagcg gaaagatggc tacctgctta
     9721 tctcagcttt atcaggagca tcgcagaggt atccgtgccg gatatgcgaa attcgagact
     9781 ttcccaatct ggaacattcc gctgaaacat ccgatcaacc tggcatatga ggcagcaacg
     9841 gctgacctga acgatgtcaa catgatcgat ccgttccatc tggaagctta tggatttacc
     9901 acggtaaact acaaccgcga tgtggaaatc ttcccggttt taaaagcaat gtttgaaaag
     9961 atctacggcg aaagccctta tcagtctccg actgacatgg gcgtcaacat ggccgggaac
    10021 tgcatctgcg atgatgcact ctgccaggaa gcttcccgcc aggagatcat ccgccgctac
    10081 ttcaacgcac tgaacggact tctgaccgga cgcaatcagg aagaggaagt ttataaaatc
    10141 gagctaatca tgaaccaggc cggcgttact gcaaccgacc gcaaggttgt tccggctgca
    10201 agacgtgtcg cagaactgac cggtgctcca gctgctgcta tagaactgcc ggacggcagg
    10261 atcatcaccg gaaagacttc tgacctgctc ggtgccgctt ctgcggttct gttaaatgcc
    10321 ttaaaagagc ttgccggcat tgaccacgag aaaaatatca ttgcaccaga atctctggag
    10381 ccgatccagc gtctgaaaac tgcttacctc ggaagccgca atccgagact gcataccgat
    10441 gaggtcctga tcgccctgtc catcagtgcc gccgctcatg aagatgctgc tctggctatg
    10501 aaacagctgc cgaaacttgc cgaatgccag gcacatactt ctgttatgct ctccccggtg
    10561 gatatcaaaa agttgaaaca gctcggtatc cagttcacga tggaagcaaa atatgagaac
    10621 aacaaaaact atcactaaaa tttctttgca cttttttgaa aaatccagta tgatttactg
    10681 gatttttttc tttgcatagt gtataatctt ttccgtattt tttttaggaa cttaatatct
    10741 ggtcaaaaaa aatgcgtgtt ttgtgcacag aacggaggaa attatgtcag taaaatatgt
    10801 atttgtaaca ggaggtgttg tttccggact gggaaagggt atcacagccg catccctcgg
    10861 acgacttctg aaagcacgtg gctacaaagt aacgatgcag aaattcgatc cttatatcaa
    10921 tatcgacccg ggaacgatga atccgatcca gcatggtgag gtatttgtaa ccgacgacgg
    10981 tgctgagaca gacctggacc ttggacatta tgagcgtttt atcgacgaaa gtctgacgaa
    11041 aaattccaat gtcacaaccg gtaagattta ctggtccgtt ctgcagaaag aacgccgcgg
    11101 agatttcggc ggtggaacgg tacaggtcat ccctcatatc accaatgaga tcaagagccg
    11161 tttccatcgc gattttacat cagacgaaac cagcattgcg atcattgaag ttggcggtac
    11221 ggtcggcgat atcgaaagtc agccgttcct ggaatccatc cgtcagttcc agcacgatgt
    11281 cggacatgaa aatgcgatcc tgatccacgt tactctgatc ccttacctgc gtgcctcagg
    11341 tgaaatgaaa accaagccga cacaggccag cgtaaaggag ctccagggta tgggaattca
    11401 gccggatgtg atcgtgtgcc gttccgagct gccgcttgac aatggcatca aggacaagat
    11461 tgcactgttc tgtaacgtgc caagtgacca tgttctgcag aatcttgatg tggaatatct
    11521 ctacgaagca ccgcttgcca tggaaaaaga acatctggca caggttgtct gcgactgcct
    11581 gaagcttgac tgcccggatc cggatctgga agactggaaa gctatggtaa atgctctgcg
    11641 tcatccaaca aaagaagtaa atatcgctct ggtaggcaaa tatatccagc tgcacgatgc
    11701 ttacatcagt gttgtggagg ccctgaaaca cggcggtatc gcacagcatg cgactgtaaa
    11761 tatcaagtgg gtggattccg aactgctcaa caacagtaac gtcgaagaag tgctcggtga
    11821 tatggatggc atcctggtcc ctggcggttt cggtgaccgt ggcatcgaag gcaagatcac
    11881 ggcaatcgaa tttgcccgta tccgcaagat cccattcctt gggctctgcc ttggcatgca
    11941 gttatcggtt gttgaattcg cccgccatgt ggttggctac aatgacgcac acagcatcga
    12001 actggatcct aatactaccc atccggtcat tgcacttctg ccggagcagg acggcatcga
    12061 ggatatcggc ggtaccttaa gacttggttc ttatccttgt gtactggata aatcttccct
    12121 ggcttataag ctttacggtg aagaaacgat tcatgaacgt catcgtcatc gttatgaatt
    12181 taacaacgat taccgcaaag tgctcacaga aaacggcatg atgctctccg gtatttctcc
    12241 ggatggacgt atcgtggaaa tggtagaact tccggatcac ccgttcttca tcggaacaca
    12301 ggctcatccg gaactgaagt cccgcccgaa ccgtccgcat ccgctgttcc gtggattcgt
    12361 agaagccgct gtcgcaaaac acgaagcaga atagatatga tgccaaggat tgtgggagtg
    12421 cgacgcacgg aacaatccgc aggcatcata aatctcacat cacaaagtaa cgaaaagctg
    12481 gggtatatct ttcgattata ctccagcttt atttcatatc aagcggatat tcgcaatccg
    12541 ctttgctact tggatccgta aaatccactt agcggtaaaa ggcattgtac acgcctccgc
    12601 tgtacatagc cacactgcgg tattttgcca gttcactgac cgtaactaac tgataaccgg
    12661 atgcgatcag tttcgggatc agcacttctg ctgcatccac agacgggctg tggatatcgt
    12721 gcatcaggat gatcgaaccg tctttcactt cattcataac cacatttatc gtattcttgg
    12781 cgtttcttgt cttccagtcc agggtatcaa tgctccacag gatcagcggt gctcccgcat
    12841 tgatctgcac attgctgttg taggctccat acggcggacg caccagcgtc gcattgtggc
    12901 cggtgagtgc acggatctga gcatttgtac tgctgatctc actctgtatg gaactgccgt
    12961 ttaaagatga aaggcttgca t
//
LOCUS       DAWDWH010000034        12650 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_2703_length_12650_cov_12.115125, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000034 DAWDWH010000000
VERSION     DAWDWH010000034.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 12650)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12650)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12650
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2703_length_12650_cov_12.115125"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    777..786
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10287..10296
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10776..10785
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3936 a         2448 c         2891 g         3345 t
ORIGIN      
        1 gaaccgaaca ggaaacgatg cgtgtcagga gattgttttc gaatcatcat ggcgtactat
       61 ttttacaaaa ctatttttat ggcgttcatt tgcacattaa gaaaatctcc gccacaactt
      121 tttattgttg acatctctca cagtacacac tgcttctgcc gccgatcgtg gtgcggcaaa
      181 gggtgctgcc gcaaaccggg catggttctc cgctgtgtcc gtaaattcta agaaacggtg
      241 tatttcgata atctttccct tttgtctcca gataatcttc cgcagaaatc gcatttttct
      301 caataaaata ttccaggcgt tccgtgatcg taacagcaag gcatttccat tctttgtctg
      361 ttaagctgtt tgccgggcgg gacggaagaa ttttcgcggc aaataaaatt tcatccgaat
      421 agatatttcc aattccggcg atgatgttct gatcgagcag gcaggtcttg atcgcttttt
      481 ttcgtgtgcc cagatatgtc tttaaatagt ctgcggaaag tttgggatca aatggttcga
      541 ttccaagctt ggcaattccg ctgaacgtat ctttttcctc ctttttcaga aaccagaaac
      601 gaccaaatcg ccgcatgtca gaaaagagca gtttctgtcc ctgatcaaag tgaaagatca
      661 ggtgtgtgta cttttcttcc gggtagtttt ttggtgtgaa cagaaggcct ccggtcatac
      721 gaagatgcag gatcacacgg tcaccgcttt tcatacagat gatcagaaat tttccgnnnn
      781 nnnnnntttc cgcggcgttg tatatgtgaa accatctgtc cggtcagtcc gaggcaaaaa
      841 tcatccacgg aagggtaagc gactgcatct ttgtgattta cagtaacttt ttcaatggtg
      901 cggttttgaa gctgtggctc cagaacacgg cgtatcgttt ctacttctgg taattccggc
      961 atgtcagatg tcctctctat cttctgattt tttatttaaa ttattatact gcttttcctt
     1021 tgaacttacc attataaaaa taaagagaat gacatcgaca taccaaatac gaataacaca
     1081 ctcacttgca atattgccca gcatttgatt gttccgcaaa ttgacaggaa tttacttaag
     1141 gtatagaatg aaaatgattc atgcaaaaga catgaaaaat ttcaaaggag agaaaaaagg
     1201 tggaagaaaa acaggaaaat ctcctgcaaa ataccatgca ggaaaaagca aagtattacg
     1261 gaaaccggat ctggcgtgat atgtcaagcc ttggaaaatg gctgatcctt gcagttgtga
     1321 ccggctgtat tgtcggtgct gcgagcagtt tgttttccta tgttctgaaa accgtgacca
     1381 atgtccggga agagcactta tggatctttt atctgcttcc ggtgtccggt ctgatcatcg
     1441 tatttttgta tcagaaattc ggaaaagatg acggaggtcc gaatcaggtg tttatgacga
     1501 tccgttccaa agatgatgta ccgctcaaat cggcaccgct tatttttata tcaactgccc
     1561 ttacacatct gactggcgga tcagccggac gagaaggtgc agtgatccag cttggcggca
     1621 gtatcggaaa tcagcttgga cgtatgctgc cactggatga agaagaccgt cacgttatgg
     1681 taatgtgcgg tatgagtgcc gcttttgcag cattgtttgg aacaccgatg gcagcagccg
     1741 tctttgcgat ggaggtagtc agtgttggtg ttatgtatta taccgcactg gttccttgta
     1801 ttattgcatc tctgatcgcg tctggatttg cagcatccat gggcatcaac ccggaggcct
     1861 ttcatgtgac gagtattccg gcacttacga tcgaaagcgg aatcaaaatg ggtatcgtgg
     1921 cactttgctg tgccggactc agtattttgt tctgcatggc actgaacggg atttccgctt
     1981 tttatgcgaa atatctgaaa aacgtctatg tccgcgttgt tgttgccagc gttgcaatta
     2041 tcgtgatcac aaagcttctc ggaaccaccg actacatggg agccggtacc ggactgatcg
     2101 cacaggcgat tgaacacgga aatgtcagaa cactggattt cttctggaaa ctggtactta
     2161 ccgtactgac catgcgtgcc ggattccgtg gcggagaaat cgtgccgtcg ttctgtatcg
     2221 gagccacttt tggctgtgtc cttggacata tcctgggact ttccccatcc atctgtgcgg
     2281 caagcggaat ggtagcactg ttctgtggcg tgacaaactg cccgctgaca tccattctga
     2341 ttgcattcga gctgtttgga tttgaagggg tttcatacta tctgattgca gtatcgatca
     2401 gctatgcagc atccggatat tatggactgt ataaagatca gacgattgtg tattcaaaat
     2461 acaaagcaaa atatgtgaac catcatacga agatgtaaga ggtataaaaa agctggcact
     2521 ttaacgaagc attattattg taaagtacca gctttttata aaaataagat tattctgtaa
     2581 aagtatagcg aacaatatat cccttggaat cgccggttgt tataataaca tccccggaag
     2641 tatttttgga tacatccatt accgggaaaa ttccatgctc tttgacataa gcttccgggc
     2701 tttctgcctc tgcttcgata atttcgtttt gagcatgttt ggttccgccg caaggattga
     2761 tcgtttcgat gataatttca tattctttca tgttgaatcc cccatttctg tactctgaaa
     2821 attataatgt ttcattctcc agacagttct gatacataga acggagcaaa aattttccgg
     2881 tttctgtttt aaatatctta tcgtacatat ttttgatatt atgtccggaa aaatggcttt
     2941 cgtccgcatt gaccagatgg tctgcttcaa tgatgatctg atagtcgatg ccatcaatct
     3001 gatcgagcgt atgatgatga cttacgagaa aagagattcg atctgtaaaa tcctgcggca
     3061 gatttgaatc tttaaaaaat tctgcaatca aagcagcact ttccttttcc tgatttttgc
     3121 cgttcgtatt tccatatttt acccgacaga gcgggcaggc aatatcgtct accagtttct
     3181 gagtgtccgt atcgagctgt tctccgtctg caatcatctt tgcataagcg taaactttca
     3241 tgaagtgatt gatgtcatga agatttccgt gagaatagtc gatcatcttt tttgcagttt
     3301 ccagtaccgt cacagtgctc acctccatat gaaaagatgt tgtggtcaaa aggtcttttg
     3361 aacctggcat cataaaattg ttatagcaca aaaatcacag attctcaata aagcagatga
     3421 aacaaatcaa aaaatgaaac aacaaaattt gacatttgat tctcaatttg ctatagttat
     3481 atcggtcaga aaacagaata taacataaat gagagtgaac gggagttcat gaaggggtac
     3541 accaccgcgg gtgtatttct ttgtgaactc ttttttgtta tagcgatgag aaaaggcagc
     3601 atgcttgaac gagtgtattt aatgattgct aatcatctga taaaaatcgt aaatcatgtt
     3661 ttagcgttgt attctggcaa tttagaaaac tttggcaatg tgcaaaatga acaattccca
     3721 agaaggcact tttacgtgcc cttcggggaa tttgttcatt tgtacatttc aaaaatattc
     3781 aaaaatatgc acaaatcttg aaaggaggca ccttatggtt acaataaacg gaaaccagac
     3841 aactctttcc acagacctca cagttgcaga atatctggag cagaataact accagacaaa
     3901 ccgcattgtt gtggaattaa atgaagaaat tcttccgaaa tatctttatt cggaaacgag
     3961 attaaaagac ggcgacagta tggagattgt tacctttgtt ggagggggct gaaaatgaac
     4021 gagaaactga caaaagaaga aatcatggcg gcactaaacg aacgccattc tccggaaaaa
     4081 caggcattgc tgtcagaggg gcgtgtggcg attgcaggac ttggcgggct tggttccaat
     4141 gtagcatatg cacttgcaag aatcggtgtc ggccatctgc atctgattga ttttgatgtc
     4201 gtggatatca caaacctgaa ccgtcagcaa tattttatgg agcatctggg aatgtacaaa
     4261 acggatgccc tgaaatcgct gcttctgaag attaatccgt atctggatat ccagacggac
     4321 tgtgtaaaag tgacagaaga aaatattgtg gatctgctga gggacgattc gatcatctgc
     4381 gaagcgttcg atcggccgga agcaaaagcg atgctggtca atggtgtgct ggaacatttc
     4441 ccggaaaaaa agctggtatc ggcaaccgga atggcaggtt atggcagcag caacacgatc
     4501 cgtacgaaac gtctgatgaa aaacttttat ctgtgcggag acggtgtgac agagccggag
     4561 tacggaaatg gtctgatggc accgcgtgtg gcaatctgtg cggcacacga agcaaatatg
     4621 ataacacgtc tgatattagg cgaagaagaa atctgacaat gttaaaagaa aaagatgcca
     4681 tacgagggcg ttaatgttat gtaaagaaag gaaaaatcat gaacacaaaa gacgataaat
     4741 ttattctggg aggacacgag tttacttccc gttttatttt aggatccgga aaattttcac
     4801 tggatcttgt aaaagcctgt attgaaaaag cagatgccca gatcatcaca ctggctctgc
     4861 gccgtgcaaa cgaaggcggt ctggctaata ttctggatta tattccggat gatgtcacac
     4921 tgcttccgaa tacttccggg gcaagaaatg caaaggaagc ggtccacatc gcaagactgt
     4981 caagagaact cggatgcggc gatttcgtga aagttgaagt agtacatgat tcaaaatatc
     5041 tgcttccgga caattatgag acgatcaaag cgaccgagat tctggcaaaa gaaggatttg
     5101 ttgtcatgcc gtacatgtat ccggatctga acgcagcaag agcacttgca gatgccggtg
     5161 cagcctgcat tatgccgctt ggtgcaccga tcggttccaa caaaggactt tgcacaaaag
     5221 aatttatcca gattctgatt gacgagatga agctcccgat cattgtagat gccggtatcg
     5281 gaagaccgtc ccaggcatgt gaagcgatgg agatgggagc agctgcggta atggcaaaca
     5341 cggcgattgc aaccgcaggc gatgttccgg cgatggcaga agcgtttaaa aaggcaatcg
     5401 aagccggaag aagtgcatac ctttccgggc ttggcagaac gatcgaacgt ggtgcaagtg
     5461 catcttctcc gctgaccgga tttctgcagg attaggaggc agccaccatg gaaaacacgc
     5521 atgtaaatga aagtatttta agtgctgaaa ttctggaaga ccagaaaaaa aaccgcatcg
     5581 accatatgaa atacctgaaa ggcatggaag atatcggttc cgaagtcctg gataaagtag
     5641 tcagcgaaat ggaagcttat gattatgata aatataacga aacagatgta agaagagcac
     5701 tggcacacag tgagcgtact ccggaggatt tcagggcact tttatcaccg gcagctttgc
     5761 cgcttcttga ggagattgcc caggcagccc agatggagac gagaaagcat ttcggaaaca
     5821 gcgtctatat gttcactcca atctacattg caaactactg tgaaaattat tgtatttatt
     5881 gtgggttcaa ctgtcacaat aagatcaacc gtgcacagtt aaatgcagaa gaaatcgaaa
     5941 aagaaatggc agcgatcgca aaaaccggtc tgcaggaaat tctgatcctg accggcgaga
     6001 gccgcagcaa atctaccgtg gaatatatcg gagaagcctg caagatcgca cagaaatatt
     6061 tcaaggtgat cggtcttgag atttatccgg tcaattctga tgaatacgct tatcttcacg
     6121 aatgtggagc agattatgta acagtatttc aggaaacgta caattccgat aaatacgaga
     6181 cgctgcatct ggcaggacac aaacgcattt atccgtaccg tgtcaatgct caggagcgtg
     6241 cattgatggg cggcatgcga ggcgttggat tcggagcact gttaggtctg gatgatttcc
     6301 gcaaagatgc atttgcgacc ggttatcatg catggctttt acagagaaaa tatccgcatg
     6361 cagaaattgc attttcctgt ccgcggcttc gcccgatcat caacaacgac cgcatcaatc
     6421 cgatggatgt ccatgaacgg cagcttctgc aggtagtctg tgcctatcgt ctgtttatgc
     6481 cgtttgcgag catcaccgta tcgacaagag aatgtgcgag agtccgtgac aatctggtcg
     6541 gaatcgcagc aaccaagatt tcagcgggcg tcagtaccgg aatcggcagc cacgtggaag
     6601 atatcgagga caaaggggac gaccagttcg aaatttccga cggcagatcg gtcgaagaag
     6661 tttacgatgc attattagca catgatcttc agccggtgat gtcggattat atctatgtat
     6721 agggaatatg gtcaggttgt ggcagttaca aaccgcagcc tgacagagcg tccttttctg
     6781 gaacagatcc gcagagtctg tgaaatgcat ccggatgcgt tgatcctgcg ggaaaaagat
     6841 ctctcagaaa aagaatatga agaactggcg aaggaagtga tggcaatctg tgcagaatat
     6901 aaggttccat gtattttgca tacattctgg agagtcgcag aaaaactgaa atgtgatacc
     6961 gtacatctgc cgcttccgat tctgcgtcag tttgttctgg aaaaacaaaa tcagaaaaat
     7021 caaaaatacc gggagtctca gaatttgagt ataccggaga gccagagaga gattatccag
     7081 aaaatcggca catctgtcca ttccacggag caggcacagg aagcacagga acttggtgcg
     7141 tcctacgtga cggcagggca tatttatgcg acggactgca aaaaaggact gcagccacgg
     7201 ggacttgatt ttttacgtca ggtctgtgct gcagtcgatc ttccggtgta tgccatcggt
     7261 gggattaaat ttgatgagac tcagtggatg agcctgaaag agtgtggtgc agccggaggc
     7321 tgcattatgt cagggatgat gaaagcataa taatctgcaa tttttcattg caggttgaag
     7381 catttgctgt attcttcatc gctgaccgga agatgcaagg tgtcattttt ttgtgtacgg
     7441 cagaaactta tataagacca agattatcac aaacgtcagt atttcagatg caagaaaaga
     7501 tatccacacg ccgtcgatgc cgaaccacct ggaaagaagg atcgcacaga tggtgatcgc
     7561 aacggctcct cgcataacag atccgacgat tgccggcagg ctgtcatcaa ccgctgaaaa
     7621 ataagacacc agcaaaatgt tgatcccggc gagcagaaat ccgagaaaat acagtctcat
     7681 tccactctga gcatacgtga taagctgaag attctgttca ctgttaaaaa tggcgatcag
     7741 cgtatcagtc atgccccatg cacagatcac gaccagtgcc tctacaatca ggcaggtcag
     7801 gatgccgtag cgtaggattt tatggacatc tttgctgttt ccggcaccgt aactttcact
     7861 gaaaagcggt tgtgttccct gggcgagccc attaaaaaca gataccgcaa cgattgaata
     7921 attagctacc acaccgtagg ctgccacgcc gatatttccg gcaaggttta acagcagcat
     7981 attgaaaaca gttgtgatca cggctgaaga aatttctccg acaaacgcag aaataccaag
     8041 ctggcagcag gagatcagat gccggaaaga cggaacagaa agatgcattg ctacctggct
     8101 ttttttaccg aaaaggtgtg tgctgcatac actcatggta actaccggag agagggcagt
     8161 ggcaagagcg gcacctgcca gccccatatt catcggaaac atgaaaatat agtcgaacac
     8221 aatgttaaac aggcttccga taatcgatgc cagcatggcc cttgacggtg cgtcatcgtt
     8281 tctggtaaat gccgtgaacg tatggtttga cataaaaaac ggcgacccga gcagaacgat
     8341 tcgaagataa accgttccaa gtttcacaag acttgcatct gcaccgagta ttttcaggat
     8401 atttgccgga aataaaattc cacacagtac aaacggaatg ctgaacagaa tactccacat
     8461 cagagactgt ccgaaatagc agtccgcatt tttacctttt gattttatga tgcagtatct
     8521 tgtcgcagaa ccgattccga tcattgcccc gattgcatac agcacgccgt aaaccggcag
     8581 gatcagattc agaaccgtca gaccattcgc accggaatgc agcgaaataa aaaaagtatc
     8641 tgccagaata tagatcgata taccgatcat tccggccata ctctgagtca catatttaaa
     8701 aaattttctg aacataattt tagattcctt tttcatagtt ttgtatatga ttgatttcta
     8761 tactgcacta aggatctaaa ataggaatat atcttctgtt atgaagaaac atcatgctat
     8821 tgcctttctt ttttagaata ttctaacggt tcagttctgc aaagtcaatc tattgtgtaa
     8881 ggcttttggc aaagtaaagc cagaggatag caaagccatt ttaggacaat aaacgggaat
     8941 cctcggcaat tcaattaccg agatgaaagt gcaggagtgt gcatttctgc gtattatcct
     9001 cttgatattg tgctctgatc atagtataat atattcagtc gaatcataaa gaaaggctga
     9061 aatacaatgg atttagataa taatgcacat tcagtattcc tgctgcacta tcatcttgtg
     9121 cttgtggtca agtatcgcag acagattttt gatgatacta tgtctggcag ggcaaaagag
     9181 atctttgagt atattgctcc aaattacaat atcactcttg aagaatggaa tcacgacaaa
     9241 gaccatgtac atatactttt cagggcacac ccaaatacgg aaataagcaa gttcataaat
     9301 gcatataaaa gtgcaagcag caggcttctt aagaaggaat ttccgcagat acggcaaaag
     9361 ttgtggaaag aacgtttctg gagccagagt ttttgtctgc ttacaaccgg tggtacaccc
     9421 attagagtgc tcaaaaaata catagaaagc cagggggaaa aggaacgaaa aaggaagtga
     9481 ccgcgtggca aacagggcct ataaattcag aatatatcct aatgatgcac agaaaatttt
     9541 gttctccagg acgttcggct gtgtgaggat ggtgtacaac cactggcttg accgaaagat
     9601 caggcagtat gaagaaaaca aagccaatgt aacctacact gtttgtgcaa aagaaatggc
     9661 tgagatgaag aaaacggaag catatgcatt tttgcgggaa gtagacagta tcgcattaca
     9721 acaatcgctc cgacatcttg atacggcgtt ccagaatttt tttaaacagc caaaaacagg
     9781 tttcccaagg ttcaaatcca aaaagcgtaa taaaagcagc tattcaacga tctgcatcaa
     9841 tgggaacata accatatcga atggatattt gaaattgcct aagatcggac aagtcagatt
     9901 aaaacagcac agggctgtcc cgaatggata caggctgaaa tctgtgacag taagccagac
     9961 accaggggga aaatactatg caagtattct ttttcagtac gaagatcagg tacaggaaaa
    10021 ggagatgcag cgttttttgg gtctggattt ttccatgcac gaattatatc gtgacagtaa
    10081 tgggaatgag cctgcatatc cgaggtatta caggaaagcg gagaaaaaat tagcgagaga
    10141 acaacgcagg ctttccaaaa tgcagaaagg ttcgaacaac cgcggcaagc agaggatcaa
    10201 gctggcaaaa ctccacgaaa aggtttccaa ccaacgaaaa gactttttac ataaacaatc
    10261 aagacagata accaatgcct atgattnnnn nnnnnngatc tggatatgaa agcaatgtcg
    10321 cggtcgctga attttggaaa atccgtttcg gataacggat gggggatgtt taccacattc
    10381 ttaaaataca aactggagga acaggggaaa aagcttgtga aggtggataa atttttcgca
    10441 agcagtcaga tctgttcagc ctgtggttac aagaacacaa aaacaaaaaa tcttgctctc
    10501 agaaagtggg attgtccgca gtgcgggatt catcacgaca gagatgtaaa tgcggctatc
    10561 aatatcagaa atgagggaat gcggttagta attgcatgac ggaaactcaa tagaacagaa
    10621 accgtgggac acacgggatt agctcgctta tgcttagcac actggtgcta tcgaacgaga
    10681 accaaatccg ccgaggtggg ggaggatgtc ataaaagaga taatattcga taaaatagaa
    10741 ctacagcaat ttcccggcac aaatcaaagt atttgnnnnn nnnnngctat gacacacagc
    10801 tgctgatcga aagaagagac gaagatctgg atgaggatga aatttcagat tacatcaccg
    10861 aacatttcga aggaaacagc ctgatcgcag ccggagatga agatctggta aaaatccatt
    10921 tccatacaaa tgagccatgg aaaattttag agtactgcaa ctctgtggga gaaatctttg
    10981 acattgtcgt agaagatatg atccgccagg caaacggtca gcagggctga aaaagatatt
    11041 tatgagagcg tttattcatc tcttaaaaat ggtggataga cgctcttttt tggttggtga
    11101 tattgttgaa tgtttctgtt catgttaaaa tgaaaaaagt gttttggagg tgttataatg
    11161 gataatgaca gagcttatct tagtaatgat aaggatatga taaaatttca atatgaagag
    11221 aaaattattc gttttcgtgg accatattca ttagaacgtt ttacatctgt gaaagaatgg
    11281 gataatggct atctggtagt aatggcgaaa tatcaacaca acaaagagtc agaagaagaa
    11341 tatattgatc taattccgat tttaaagaat ttatatattg atgctgttca ttttttgaaa
    11401 cctattaagg aagtgaggtt agcttatgga caaaattaag gaaattgcag ataaagcaga
    11461 catgattgta aatggctatg catttacaag agaaaatgat cagattagaa ttttaaatct
    11521 gaataatcta gacaaggcat tagtaatatc cgaggacgga aaggtactgg aaaccactat
    11581 ggatgatatt gaaattcgta ttgttttgga ttattggaat agtgaccgcg aatttatgga
    11641 ggatgaagat gcctaaatat tatcaggata aaatatgtgg attttatttg tagacagtat
    11701 aagtaactta taataagcat ttatttgtat tttgtatatt aacagatttg ctgaatttca
    11761 cgcataattc cctcatgttt tcacatacta tccatacagt gcaaaatatg gaaaagcatg
    11821 aagaatacag gaggaaacag ataaatgaaa gcgacaggaa ttgttcggcg tattgatgat
    11881 ctgggaagag ttgtcattcc aaaagaaatt cgcagaacat tgagaatcaa agaaggagac
    11941 ccccatcata aaacgttgac accgtttgaa aagtactgta ttgcaatgct tcgcttcgcc
    12001 gaacgctgca tttacaaata aatcaaacgg aggaaaagac aatgacaatt tacgaaaaac
    12061 aaggcggtac atatcgcaag gtgggagatt atcgcttgcc aaatctcaaa ctcaacacag
    12121 agcaaaacgc ccaaataggc gtgtggggaa tgaggcacaa gcgatacttg aaacaacgtc
    12181 acaaagtgat ttattacaat cttttgacgg caggcatact taattcatat cttgccgaaa
    12241 tcgaccaaag agcaaaattg ctattcgacg aaacagtaaa atcacttgcc aaaaaggaaa
    12301 atgttaccga aaaactcaaa gaaaccgata tgatgttgtg ggtgcaaaag atgaataata
    12361 tccgcaaccg agcagcggaa attgtgaatg gtgaagtgat ataccacatc taaattactt
    12421 aaagaagagt atcgttttga tattcttctt ttttcatttg ctgttaaatc taaaatgtag
    12481 tataataaaa atattgattc aacatctaaa gggtgatagt ttgaatacaa agagtaatat
    12541 gataattgca aacggaagaa tagttactcc tgatataaag ttttgtaatt ataacaacac
    12601 aactcataag tacgatataa tttttaataa cggtaaagtg tacagttaca
//
LOCUS       DAWDWH010000035        12552 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_2727_length_12552_cov_10.272145, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000035 DAWDWH010000000
VERSION     DAWDWH010000035.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 12552)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12552)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12552
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2727_length_12552_cov_10.272145"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1603..1612
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5853..5862
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7925..7938
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11353..11362
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3121 a         3263 c         2377 g         3747 t
ORIGIN      
        1 ataacctccc ataccgcatt tgaaacggct taatcgaact ttttaaatta tagcatactt
       61 tctataaact tacaaatact tattttcaaa ttccacaata cgacaaatcc tgtcctcaaa
      121 accgaaaaaa cttcttattt tttatagaca tttgtcagaa gaagagatac actgttacca
      181 acatagcata agtcagcgga cagatgggat aacttgtatc tttctctgtt tatccgtctg
      241 cttatagtat acgtggaagg aggttttatt tatggatact ttacatattg ctgtttgtga
      301 taatcatgtt tcttccctga cttttatcac cagtgagatc accaagcaga ttatgaacat
      361 gaatgtccag gcagatgttg cttccttttc ttccggcgaa gatgtcatgt gccgtgtatc
      421 ggaaggaatg ttttttgacc tgtacatttt aaatattgaa atgccaaagc tcgatggcat
      481 cacacttgct ctgcagatcc gcaggcatca gaagaatccg gtcatcatct ttgtatctgc
      541 aagagaagaa tatgtttttg attctttcaa agcacatcct tacagtttcg tccgcaaaga
      601 tcattttcat gaagatttga gttccacact gaaagcattc atgctggaat accataaacg
      661 ggaagactcg tttctttata ttctccgcac accggatgcc ctgcacaagt taaatctcag
      721 acagatcctc tacatccggg atatccgaga tggttttatt gagcttttga aaaacgacgg
      781 cactgccaag cggatccgct ataacataaa tgacctggaa aaagagctgg agccttgtga
      841 ttttgtccgc atccatcctc tggagctggt caactgcgac tatgttttcc ggatcaaaaa
      901 cggaaaaatt atgcttgata acggaacaga acttgcgatt gccgatcctt accgggaaaa
      961 tatcctggaa aaattcggtg aaatatagcc gctctttttc tcagcagcag aacataaaat
     1021 agccaagaag cagcagcccg aagataaaga gaaccgacac aaagacattc ggcataaaaa
     1081 atgcgatcgt cataccaaat gcgatccaga acacggtata gcctatgatc cttttcattg
     1141 gatttctccg aatctcttta ttaccagaat atgcatctgc aaaggcatcg tagcacaaaa
     1201 ggtcatggat tttacggaac tgtccgatcc ccatgacctt ttgttatctt tttctttatt
     1261 ctttttcttc ttttgcctct gaaatgatct cgtcgtcatc ttttgatgag aaaaattttc
     1321 catcggtaaa tttattaaca aaatccggaa ccatatccag gatcttggtc agaccgtcct
     1381 gatttttgac attgaccagt ttggtcgtcc cgttctggat cacaagcacg gcactcggac
     1441 tcatttttcc gcccatgcca ccgccgccgt tctgtttctt gtcttcattt tttgctgtcg
     1501 cagcaacacc aaacgtgaca tctacaagtg gcaggatgat cgtgtcaccc actgtgatcg
     1561 catctcccac aacggttttg gtcgtgataa aactgtccat gcnnnnnnnn nnattaagcc
     1621 ctccttaatc gtctgaatcg tttccagtat atttttaatt ttttatcaaa aaatatcttc
     1681 gctcctgtca gtgcaagatg gcagatgcgg atatgtccca aagccaaaac ttctccttcg
     1741 cagacctgat cttcaaaatc aggctcaaga tccagttcct cgcacttcgc cggaaggatc
     1801 agatagagaa tcccggtaaa ctctcctgtg agtgccggat ctccgaaacc ataatgtaca
     1861 tagcctttga tcttatgcgg gcggatatgt ctcagaagat aaaagagatg attcttcaag
     1921 atctgataaa cttcccgtga aagatctgaa cgcagaaaat caagatatcg cttcagtgtc
     1981 tcttttgatt ttgtaataga acggatcgtt tttttcgcct gctcttttgc ggtgaggata
     2041 cgatttctga tcttttcgat ctgcatgcgt accttttcga ttatattgaa aaaacgatgt
     2101 cgcttttccg gttcagccgc atcttctgcc tgcaattctg acatttcacc atcctgtgtt
     2161 tctgatttcg aagccttgtt ttcctgcgtt tcggttttcg gagacggttc tgggtccggt
     2221 ttcgttttct cttctaacac aggcagtggc ttcttctctt ccgaaacatc cgctgttttt
     2281 tctgtctcgc agttctctgt ttcattgttt tctgcctctt ccgaagaatc ctccgtctgc
     2341 ccggtatttt ttttagcaaa atcgatcttt tttccaaaaa tctgcggtac aaaggatatc
     2401 tgcccattcc ggtagcctgc ttttaccttc aggatgccaa acagccagct cacactgacg
     2461 tttgcagtac tttttttcag ttccccgtgt gtttctgcac atatgcgata ccgcagaggc
     2521 aaaaacaaaa cggtcagaag gatcagcagg accagtccca gaagaacggc cagaagaatc
     2581 cccactattt tcagtatagc cagtatgata tgtatcatat acaaacaccc gttaatttct
     2641 ttcctgatta tgattctgca acgcctgcca gaatcacctg ccagacctca cataagcctg
     2701 ccctgtcttt cactgcatct gcttttcaga taacggagga tatctccgtt tccggtttct
     2761 ctgtatgtct cttccgccat ctggaccacc agatccacgg cttcctcata gcctttggca
     2821 atgccgtaga tcatcggcag cttttttctc actgctcttt gtttcagcca cgcagaagaa
     2881 aggatatcaa actgatcctc gccgtttgct gcctcggtga tcacccacac atcgatcatt
     2941 cccgcaccga tcttcaattt ccggatgatc ttgtttttct tttttgatgc accttccccc
     3001 acatagagat atggatacca tttcatattt tttcctttct cctgccatcg aattcttgtt
     3061 ctattttacc agacacagaa acataatgca acgactaacg aacttctctt atcatgcagt
     3121 actgtttcag ggagaaattt ttttcaagca caccatatac cttaaatcca aatttctgat
     3181 aaaactccac attattctga ttgtgtgtct ccagcgagat catcatccgg tgctgatctg
     3241 cgtagcgtat ggtttcctcc agaagtcctg ccgcaatccc atgatgctgc atctgcggat
     3301 taactgccag atagatcacg tggagcctgc gattctgatg aagctgatcc gtccagttcg
     3361 agttcagata gtctccgccc ttgataaaat tgtaaaatgt gtgcagagac ggatcttctt
     3421 ttatcagaaa actatccgta tgtaatactg cttttgcttc tgtataaaga aagcggaaaa
     3481 tattgcagga ttttgtttca tcagatacca caagaacgcc gttcagctct tcgctgtccg
     3541 caaaaatctc acatgtctca taaaattcat ccatatcaca gtgaaacaat tccggcatca
     3601 gtttctttcg gatttccgca tttggtatca gtgtctcata aagtggatca tgtgcaaagc
     3661 acaggttcag aagtttttcc agttttggca gatcctctct ctgcacgcgg tataaatttt
     3721 cgctgtccat aaatttctga ttttgtccct ttctgtatgc tcagttcaga tacgtttctt
     3781 tcagaacttc tgttatcttg tcagtatatt tttccagtaa tgtatactgt ttttcacata
     3841 cttttttcag tcggagaatc tcactttcaa aatcagatcc cgcagttttt tctttttcca
     3901 gcaggcttct caccagagta taaagctttt catgctccat gatccttcca tagccccatt
     3961 tcagccgttc ccttggctga tggaaaacat agtccgtgaa atgattgtat acatcttcat
     4021 caaacataaa cgcataagca ttgttgtcaa cctgatagag ccgtccaagt gcctttgata
     4081 cttctgcttt ttcaagatct tctatcgcat cttctaaaag cctgatattt ttcagaagga
     4141 tctcatgtgg ataaagtacg tttccgtacc agtcgatgcg tacaaacgta tcctgttctt
     4201 ttttgaagtt tgcaagctgt ttctgttcca gaggacgcag gctttcaaac agctcttctg
     4261 cttcttcaaa gcgttcctcc ttaagaaggc ggcggtaagc acggttctgt tcctgtactt
     4321 tctgataatc ctgctctgct tttttttctg cttctttcag atattgcttc ataacatcca
     4381 gtttctttga aagtacacct tttgcagctt ccgctccggg aaacttccct gcccggctca
     4441 tgaccggggc aaaacacaac ggaacgaccg ccgtctgatc cagttcacag atcagcagca
     4501 gaaacagttc gtgatgcagg cggtatacct gctcatcgta ataatcatcg ttatcaaact
     4561 gtgaatggta gtgcgtctcc ataaaacttc caccggtaaa atcattgacc atggacggaa
     4621 tgccggcgat cgccatggaa aagtcatccg accaggtctc gatcggtgcc gtaatcttcg
     4681 tctcctccgg ataggcctgt gtcagaagcg gcagattaga aagaaattct tccagataac
     4741 gcacatattc ataacagctg cggatcctgg cccttgttcc atgtgccagt gcaggaagtt
     4801 caaaattaag atctgcgatc actttgccga cccactccgg atgtgctgta aaaatctgct
     4861 cgtaagcacc cgctgaccag tcaaaatcag aatcgatcac gccccattcc tctgccgcca
     4921 ttgcacagaa tacgattgta ttgtttggct gataaccact ctccagcaaa gactttgcga
     4981 tgccgaacat cagggcaacc gcagtattgt catcctgaaa gccgctgaaa taagaatcat
     5041 aatgtgcgga gagcagaatc atacgctccg gatgctttcc aggaatcctt ccgcagatat
     5101 tgtaggtggt gcacgacggc atcacctggc tgtccgcgtc aaaaaagacc tggatctctt
     5161 ctttttcttt caacagctct ttaagcactt ctgcatcttt tctggagatt gaaaaagctg
     5221 ccgcatcgga aggtcctgca atatcctgtg catttaaggc ttcgttgtca atctcaccat
     5281 atccgccgtc ctggacagcg atcagggctt ttgccccttt taaatgtgcc tgataaaccg
     5341 ggtaattgat ccaccattca tctctctggt tgatatcgac aagtaccagt ttcccctgca
     5401 cttcccggcc ctcataatcc ttttccgttc cttttcccac ataagtcagg gaaaaaggtt
     5461 caaagccatc agtcttaaag tctgtctgat atgctccaag ctgaaattcc cggcgtttgc
     5521 cttttgtctc caccgcaagg actgcctttt taaattccca gccatccacg gtgacggcat
     5581 cttttgtaac ctgtgaaagc ccgatgcttt ccatttcttt tttcagcatc tcaccggtct
     5641 taaattctgc ctgcgaacct gccgggcggt atcccagtac ctcgttggag cgaaactgct
     5701 ccatgcgtct tgccagtttg taagaatatt caatatccag atgttctaat aaatgttctc
     5761 ttgtatgcat atccagacct cctatttgat ctacccacac gcatttttat cttagtcgga
     5821 gaagactccc atttctataa gaatgtctgc aannnnnnnn nngtctgcaa agtccatgat
     5881 atgaaaatgg agtctgcggc gaaccgagac tccagctttc actccttctg gtgcagtaat
     5941 ttgtcaaaaa tattttttgt taccccagag attccgcctc tttaattcca gatattcatt
     6001 atacgctttt tccaaaatcg ctttttcatt tgaaaacttc agcagatgat atgcctcatg
     6061 gtacttataa tatccggaat ccacgaaata ttcttctctc tgtggcttgc tgtaataact
     6121 gtccgccatc atcagatacc agtgtacatc cttggaaacg atgtcctccg ggacattgaa
     6181 tgtatactgg ctttttccga catacttgat aatggaagca tcgacacccg tttcctccaa
     6241 cacttccctg gcagcggtat ctttatactc ttcgccagct tctacagttc ctttcggcaa
     6301 gacccagccc tcatacttgt ttttgtagct tttgtacaac agcagaatct tcccgcgaaa
     6361 gattaccaca ccgccacagc tcgttgcctc aatcattgca attcctcctg tatgcgttgt
     6421 gtctaaaact gtttttagta tacccctttt ttcagccgaa cgcaagctat aaaaatattc
     6481 tcttttgcat tactgctgat actgcagttc gtaccaccat ttgtcaaata ccatcttggc
     6541 tgccgggact gcggaagaac ttccggaacc gccgtcttcg atgatcacac tgatcgccag
     6601 ttttggattt tccgcatcca gataacctac aaaccaggag tgagttcctt ctgctcctcc
     6661 ttcgctgccg tattctgcag atccggtctt ccctgccgcc gtatattctt ccccggaaat
     6721 ctttcttgcc gttccgtcgg tgactacttc cttcataaat ccggacagga tatcgacttc
     6781 ttcttctgac atcagttctt tgtatttgac cggtgcatac tgctttacaa tgtctccgtc
     6841 atacgtttcg atgtgatcga tgtagtatgg attcatcata gtgccgccgt ttgccacggt
     6901 tgcagccacg agtgccatct caaacggtga cacgaccgtc tctccctgtc caatcgcagt
     6961 catcatctct tctccgtagg aggcattgga tgacagacgg aaaaggctct ggctggacgg
     7021 aagatcgatc ggcagatctt tgttaaagag gaacgactcg cagagtttcc ggtacgaatc
     7081 attgttcact tccataccga tctgtgcaaa ggcaccgttg caggagtgga caaatgcctg
     7141 ctccagattt tcatatccat ggacttctcc ttcgtagcag gtgatccgca catcttcttt
     7201 ggaaatgctt ccgttacagg tatagttgaa attctggtaa tcatccggat gttcccggat
     7261 atattccagc gttgtgagga tcttaaaggt agatcccggc ggatactgcc cctgcagtgc
     7321 acggttaaaa agggaactgt tgctgtcgtc attaatcagt gcatcccagt cctgctcgat
     7381 cgtattcgga tcaaaatccg gtttggaaac catcgccagt acctttccgg tggacggctc
     7441 cagcaccacg atcgcacctt tgttgtcacc aagtgcatca taacatacct gctgcagctg
     7501 tgtgttcagt gtcagcacaa ggctgtcccc ctgtactttg gtatccagct tttcttcccg
     7561 gatctgatcc aggatggagg catgactgct cagcagatca taattatgga ccgactcgat
     7621 accggactta ccatttgttg cataaccgac aatatgtgca aacagattgc tgtacggata
     7681 gcttctgctc tctgtcccgt cttctgcaat gtctgtctgt gccaggacgt tgccgtccgc
     7741 agaataaata ttgcctcgga tgatctgttc ctggtaagca gactgttttg tattgtactg
     7801 gttattatta atatcatccc gcaacaccac attaaaatag atcagataac ctgccattga
     7861 gagaaacaac gccaccatca gataactgac acgaaccagc cccttgttct tgatacggcg
     7921 gatgnnnnnn nnnnnnnncc tggatcacag agaacatgat cagtgtactg agcatggaac
     7981 ttcctccgga gctgacaaac ggaagcgtga caccggtcat cgggatcatc ttcatggaac
     8041 cgccgatcgt taaaaatacc tggatcgcat acgtgcaggc aagtccaagt gccacataac
     8101 ggtaaaaatt cttcgtcatt ttcattgcga tattgacaaa catgataaag cagctcatac
     8161 agatcagaat cgtgcaaatg cagaaaatac ttcccatctc ttcggcgatc gccgcaaaca
     8221 taaagtcctg atccacataa gggatctcgg tcggtgatcc ctgatacaga ccggtaccga
     8281 accaaccgcc ggccgcgatc gcaaacagtg cctgtgccac ctgatagccg cttccttcat
     8341 aaacactgaa tggatctttc cagatctcca cgcggacacg gacatgggca aacaggaaat
     8401 acgctgccac ggctgccacc acaaacgctc cgaatccaag agccagccat ttccagtcac
     8461 gcgttgccac ataaagcatg atcagatagg tcataaaata gatcagtgca cttccaaggt
     8521 ctgtggagat cacaaggatc agtacatgga cagccgccag aatggtagtt ttgacaatct
     8581 gcgaaaattc ggtcgatttt gataacattc cggcaatgca gaatacataa ataatcttga
     8641 caaactccga tggctgcagg gtaatgccgg caatcgtcag tgacagcttt gcaccatttg
     8701 tcaccgttgc catgaccgca accgctacca gaagtccgat gccaaggata gcatagacca
     8761 cagcaaattt cgtgataaat ttcagcttgc tgatcagcca cggcacgatc agagcgatca
     8821 ccgttccgat cacacagatc tgaaactgtt tgaccgactg atcataagac agtcttgtaa
     8881 ccataataaa accaatcgtg atcagcatac acatgttatt gatcgcaagt ttggaagctt
     8941 tcggatacag atgcctgaaa aagatcagtg tcgctgccag atacaatgcc tgttccccgt
     9001 aaaaaaacag gatctgggac tggttcatct gaaggtacag taccataaaa gccacaaaat
     9061 ggagacagaa catcaccaca ttctgtctga taaagaggaa ttctctcgct tcctcgctcc
     9121 gcttagcaaa gatcagaata ctttgcagcg tatagatcac catcaaagtg atcagaacat
     9181 attttgaaaa ttctatgatc agtttatcca ccgtttcacc tactccactc ctcgtttgta
     9241 gtgtcctctt gtaaattcat atcccggctc tcccagttct tctccccaaa gatattcttt
     9301 cacaaatcgc tttgcgactg ccgccgcttc tttgttgctt tctgtcgtaa acgccagccg
     9361 tacagatgcc gggctgcatt ttcgcacctc ttcgctccat ccgtgcagcg aaaccggaac
     9421 agaattgtaa atcacattat aacagtaact gcagcagttt ttcaccggaa attcttttcc
     9481 cttgcgatca cgtaccttaa aaagatccgg cgttttctga cagtgtccgg cacttttttt
     9541 cagacactgt gtgcttgtca tgaccggaat ccttccgtag accagcattt catcgccatc
     9601 gcagtcacgc tctttcaatt ctttgaacgt aagctctgca gggcagacaa aaaaactgca
     9661 gcctagattt cttaaaaacg ctttcgcttc tttattataa gcataaacat tgtaatctgc
     9721 tgcccaagtt ccgtcatatc cgatctgctc cagatatgcg atctgctccg gatttttgac
     9781 catcattccg tcaaaacagg tcatatactg cttcatctgc tgatcccaca gttcctgatc
     9841 ttttgcccgg aacagagacg gaaaaatgta ccagagctcc tttttctgcc cctggcatct
     9901 ctgtgccatc gacggaatct gttcacacat ccgaaaatcc agaaagcatt tgctgtcaag
     9961 ataaatcctt ttgacgcctt ctatttgcag catttcttca aacaggtctg cccgttcgat
    10021 cgatacagaa agctttgctt tcttttcttg cttcccgtca ttggcagatt tttctgcttc
    10081 cactttttcc atctccgggc tgttttctcc tgttttcccg gatttgtcag attttctctg
    10141 ataagcctgc agctgttttt cctgtaatgc ttccagagcc ccccgtcgaa gttcattgaa
    10201 actctgcatc ggaagaaaac actcctcatc catcacaatc tgaagatgtt caaacagata
    10261 cggcgaattt ccggtacgca tcatctgttt tcttacacgt tcctccgcaa tcggctgttt
    10321 ctgtgccgcc tgtacaatcg ctccgctctg ggaggcagtg atcctgcctt ccggcccatc
    10381 atatgtcacc gtcagtatag caggtttccc tttaaaaatc attaacttgc ccttaatttt
    10441 ttcatagagg atcttcccga tatactgctc tttcaggcct tccatcagtt ttgcattgta
    10501 cgtgcgattc cagatgcctc ccttctgcag tctgcccgca tcgcggactt tcatggaaaa
    10561 gatcctgcct tttttcacat cttccgctac cgttgcggac tccagcatat cacctttttg
    10621 cagattttcc agtgctttaa aagtaagctg tccgcctttt ctctgttcca gaagcatggc
    10681 accttctgtc ccgtagtgat tctgccgctt catggacatc atcgacacac catggtatct
    10741 ctgataatat cctgtggaaa aaccgccccg gttatacaga tccatcaaat cttggatatc
    10801 ttttttctcc acatgatatc cttcttttcc gtttttacgg taaagatcca catatttgcg
    10861 gtacatggct gccacacctg ccgcatactc cgttcgcttc atcctgcctt cgatcttcag
    10921 agaatccaca cccgcttcca gaacctccgg caaaatctgc aaagtacaga tgtctttcgg
    10981 acttagccag aactgctctt ttccttcata tacataagga agtctgcacg gctgtgcaca
    11041 gcgtccacgg ttcccgctgc gtcctccgat catactgctc atcagacact gaccggaata
    11101 acagtaacaa agtgccccgt gcacaaaaca ttcgatttcc agagaagtat tctgccggat
    11161 cgctgcgatc tcctccaggc tgatctctct tgccggaaca actctggatg ctcccagttt
    11221 ttccaataaa gctgcacttt gcacggaatg gaccgacatc tgtgtgctgg catgaagatc
    11281 cagattggga aattctcttt tcaaaaaagc aaatgccccg aaatcctgca cgatcacggc
    11341 atccagaccc tgnnnnnnnn nngctcataa aacggcacca gccagtctgc cagttgataa
    11401 atctccgact gtttcattag cgtattggtt gtcagatata attttttatt gtgcagatga
    11461 cagtaatcga tgcattctga caactcctca tcagacagat tgtttgcata cgccctcgca
    11521 ccaaacattc tgcctcctgt atagactgca tctgcacctg catacaatgc agcttttaat
    11581 ccttcgatcg aacctgccgg tgccagaact tctgtttcca caaaatccct cctgtacttg
    11641 tcatggcaaa agccacctgt tttcatgtaa acttaaaaag gttggcagac gcaatgccga
    11701 actgcctttt ctccggctct ttgtgtcgtt ccaacctctg tctgtattac ttgcccaact
    11761 ctgtctccag ttttacaatc tgcttctggt actctgttgc ctgttctttt aaagcctcca
    11821 gctcttttgc tgcatcctcc agcttgatct gagtggagat cagttcatgt ttcaaatcgt
    11881 acagttcctg atctttctgt gccagctctt cttcgaccat ctccacctga ttcttggctt
    11941 taaaataatc atctgcaaca ttcaactcca gcaggatgct tttcaactcc tgggacagtc
    12001 ttgcgtaacc atccagtttt tttaattctg ccagtttgtt attgatatat gtggcaaccc
    12061 gctgcagata ctcttcactc tcatatccac tcagggtata aatcttccca tcaatgagta
    12121 cttccgtttt gtttttggat gacatcctgt gtacttcctc tctttcggta attacttgac
    12181 gcatgatctt tccctattat aacctcatat cgcaattatt tacaagagaa tattacaaat
    12241 ctttttcatt tcgtttcttc tttttcaatt ccaaggtgct gatacgtaag atctgtgacc
    12301 actcttcccc tcggtgtacg catgataaac ccgtttttga tcagaaacgg ctcataaact
    12361 tcttcgatcg ttccggcatc ctcaccgatt gccgctgcca gagtatccag cccgaccgga
    12421 cggcccataa atttctctgt catcgtatag aggatccggc ggtccgtctg atcaagtcca
    12481 caggaatcca cctccagaag atccagggcg aaggatgcca cttctcctgt gatctttcca
    12541 tcgtaacgca cc
//
LOCUS       DAWDWH010000036        12339 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_2769_length_12339_cov_13.839954, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000036 DAWDWH010000000
VERSION     DAWDWH010000036.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 12339)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12339)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12339
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2769_length_12339_cov_13.839954"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2944 a         3287 c         2475 g         3633 t
ORIGIN      
        1 aaggagctta tcatacatca catgccaggt tttttatcat cttttccgtc aggctgcgta
       61 ccagtgtcag cacatcctca ttatcttttc cgtttttacg cggcttcata taaataaagt
      121 aaggattggt atctatgaca aatttcattt tccctttgct ctcttttacc agaagctcga
      181 tccctgccgg atctgccttt gcccgctcaa acatggtaaa gcggatctgg tcgcctttct
      241 gtgcgatctc ggtcagccat gcccggtgtg ccatcgcttt taaatttgcc acagcaagca
      301 ggttctgcac cgcttttggc ggctctccga aacgatccat cagttcttcg agcatatcat
      361 cgtattcttc ttcgctctcg attgtggcga tgcgtttata gacatccagc ttctggaatt
      421 cattcttaat ataacgttcc gggataaaag catcaatatc cagatcgatg acggtatcaa
      481 attcttcttc cggctgcgtc tcacctttga gttcttttac ggactcgttg agcattttgc
      541 agtaaagatc gtagccgact gcctccatat ggccgtgctg ctctgccccg aggagattgc
      601 cggcaccacg gatctcaaga tcacgcatgg cgatcttgaa gccactgcca agatcggtaa
      661 attcccggat cgcatggaga cgtttttccg ccacttcccg cagcattttg tttctgcggt
      721 acatcagaaa ggcatacgcc gtccggttcg aacgtccgac acgcccgcgg agctgataaa
      781 gctgcgacag gccaagattg tccgcgtcat ggatgatcat cgtgttgaca ttggaaatat
      841 ccagtccggt ctctatgatc gtcgtggata ccagcacatc aatctcacca ttgataaagt
      901 catacatgat cttttccagt tcacgctctt tcatctgccc atgtgcaaat gccactctgg
      961 cctccggcac aagcgatgcg atcttatttg ccgtctctac gatcgtgttg acacgattat
     1021 agacatagta gacctgtccg ccccgtgcca gttcccggct gatcgcttcc cgcaccatct
     1081 cttcgttgta ttccatgaca tatgtctgga tcggcacacg ctccatcggt gcttcctcca
     1141 gaacgctcat atcgcggatc ccaaccaggc tcatatgcag cgtccgtggg atcggcgttg
     1201 ccgtcagggt cagcacatcg atgttttccc gcagcttttt aatcttttct ttgtgcgtga
     1261 caccgaaacg ctgctcctcg tcgatgatca gaagaccgag atctttgaac tgcacatctt
     1321 ttgagagcac ccgatgcgtg ccgatcaaaa tgtcaaccag tccttttttg agatcttcca
     1381 gcgttttttt ctgttctgct gccgtgcgga aacggcagag cagatccaca cgcaccggat
     1441 aatctttcat cctctggacg aaattattat aatgctgctg ggcaaggatg gtcgtcggca
     1501 ccagaaatac cacctgtttg ctctcctgtg ccgccttgaa cgccgcacgg atcgccactt
     1561 ccgtcttgcc atatccgaca tcgccgcaga tcaggcgatc catgatctta ctgctctcca
     1621 tatcgtgttt caccgcttcg atcgcctcca gctgatcgtc cgtctcatca aacggaaacg
     1681 tctcttcgaa ctctttctgc cagatcgtgt cctctccata gcagaatccc tcttcccgct
     1741 gtctcgccgc ataaagctgc accagatctt ttgcaatgtt tttgaccgca ctgcgtactt
     1801 ttgtcttggt acgcccccac tcctgcgtcc ccagtttatt cagtttcggg cgttttgcct
     1861 gcgaatctgc atatttctgg atgacatcga gctgcgttgc cggcacataa aggttgcttc
     1921 cgccggcata ctccaccttc atgtaatctt ttgtgacttt atcgacttct attttttcaa
     1981 tcccgcggta aatgccgagt ccgtggtttt catgcaccac gtaatcgcca acagaaagct
     2041 ctgaaaaact gtggatcgtc ttaccttcgt aatgtctgat ctttttgcgt tttttctttt
     2101 ctttgccgaa aatatcgctc tccgagatca ccaggaactt gatcagcgga tattcatatc
     2161 cgcggtgggc acttccgtaa agcagcatga tctcgcctcg ctgtacgatg cggtcggcat
     2221 cttccgaata aaatgcccgc agttcatgat cacgaaggtc ctctgccaga cgtttcgccc
     2281 tggttctgga tgcacacagc aggatcacgc gttctccctt tttcttccac tgctgcagat
     2341 ccttcaccag aagttcaaag ctgttgttgt aggtgttgac actgcgtaca tcgatgctga
     2401 aatgctccgc aaaatgccac tgtggtcctg ccgcctccag catacacagc cccatgcagc
     2461 gatgtttctg caatttttcg gatgccatct ggctctcaca gagcaggttc atctgtcccg
     2521 gcagcacata ccctttttcc agtcggtttg tcatgctctc ccggaactcc ttttcgatcg
     2581 cctgtgcttc ctcccagagg cggttcggct catctaacac caccagtgcc tctttcggaa
     2641 aatattccgc aaacgaaacc atccggtcat aaaaataagg agccagacca tcgagcacag
     2701 acatctgctt ccactgcgtc agactctgtt ttacttcttc tgcaaaagct ttcaggcgtg
     2761 ccgattcttc tgttttccgt tcctgaaaga actgatctgc aagccttttc gcctcttttt
     2821 ccagcaccgc aagccctttt ttgatccgcc cgtcatcagc aataaattcg cccgccggat
     2881 aaatgcggat tcggtccaga ttttcaatgg aacgctggct ttccacatca aaactgcgga
     2941 tggagtcaat ctcatcgccc cacagctcga tgcggaccgg attttcttcg gtcagcggaa
     3001 aaatgtcgag aataccgcct cgcacggcaa actgcccgct tccttccacc tgtcccactc
     3061 gttcgtagcc catggctgtc agtttctttt tccagagctc catgtcaaat tccgaatcgc
     3121 tctctaccgt ctcgatctgc ttttgaaaaa cttccggcgg cagaagatga tccatgatac
     3181 cgcccatggt cgtgacgatg gtcacttcct cttcttccat cagtttctgc agtacacgga
     3241 tccgctgctg taccagaaga ttgctctgca catcggcact gtaaaagatc agatcttttg
     3301 ccgggtaaaa cagcacattt ttatcaaaca ggcgataatt ttcacacagt tcttttgcac
     3361 gcagttcact gtatgtgacg atcagtttct tttttgccgc ccctgctgca tagataaagt
     3421 gtgctttctg ggcatcgata catcctgtca cctggattga ctgctttttc agtgcctgct
     3481 gtattttctg aaattcttca agctcctgaa gcggttctgt aaaagctctc atccgtctgc
     3541 tacctcccat tcaaacccct gtctatttct ctgtcttctt gttaaactgg ttcatcgcat
     3601 gttccatggt gtcattcatc atgcagacgg ctgcttctcc ggcccgctca aaggcttcct
     3661 ccatcagcac tctttcatcc tttggaaaac gactcagcac atagtctgcc agatcatatc
     3721 cttttggttt ctcaccgact ccgaccttca cccgtttaaa aacctgggtg ccaagatgtg
     3781 cgatgatgtt tttgatgcca ttgtggccgc cggcacttcc ttttggacgg atgcggagct
     3841 gtcccggctc caggctgatg tcatcgtaaa tgacgatcag ttcttcttcc gggtcgatct
     3901 tatagaaatc aaccagttcc cgcacactct cgccgctcaa gttcataaaa gtctgcggtt
     3961 tggcaagcac cactttctga ccttcgatca tgccttttcc gcagagtgct ttgtgttttt
     4021 ttgtatccac gctgatatta tacttgtctg ctaatatatc tatcgtgtca aacccgacat
     4081 tatgtctggt ctgtgcatac tgtttgccag gatttccaag tcctgcgatt atatacatat
     4141 ccttacctcc aacgacaatt ttgcccgcct ttttctgtgg ggggtatccc actttcaaaa
     4201 gccgggcttt ttacctgttt tgtttctttt ctttttacca acacatcata ctctaaatct
     4261 gctctttttt caagtctgtc ttcgaatttc acatttttta cacaagtggg aaggaaaacc
     4321 agaaccttac ccctgttttc tcattctcca caccatattc tccgccctgc atcgtgatca
     4381 cagtctttac aatatgaagt ccgagtccgc tgtgccctgc ctgttcttct ccctggtaat
     4441 atttttccca gatttttctt ttctcttcgt ctgtgagcgg ttcactgtcg ttgtaaacac
     4501 caaaaacagc ttttccgtct ttctttgcca gagaaatttt catgtgcctt ccctcgccgc
     4561 aatgtgcaaa agcattcata atatagtttc ccgctgcctg ctcaatgtat tcacggtttc
     4621 cgaggatcct gcagtccttt tcgatctcgc ttgcgatctt cagattcttt ttgtgaaaca
     4681 gtgcatcgta tttcaacatc atatattcta ctgtttctga taaattcaga cgttgcttct
     4741 ccacattttc cagttcatgc tcaatagaag aaatattcag cagatttccc accatatcac
     4801 tcattttctg cacttcttca cggatcgacg agaaataata atcctgatcc accttatctc
     4861 tcatcgcctg caaaagctcg atctgtccgg aaatgaccgc cagcggtgtc ttcagctcat
     4921 gtgtgacatt tgccgtgaaa tcgttccgca ttttattgaa acgctccagt gcatccttgc
     4981 tgctttccag tttctggata taatcctgca tctcatctgc catcgtattg aaattatgtg
     5041 ccagaacact gatctcctga tacgttccgt aatcttttgc ccgcacggaa aaatcacgct
     5101 cagtcagttt ctgcgaaaca ccggaaagtt cttcgatcgg ctttgcaatc cttcgcgact
     5161 gccagtacat aaatgcacaa ccgctcagaa gggaaaaaac aaccacgaac agcaaaaatt
     5221 tttcggtata gaacagggca ctgtagactt tctgtacatt ttctttcaca cacacaaaat
     5281 aacgctcatc gccctgtttc agccttccaa ataaccataa cgtttctttt cctcttgcat
     5341 tccagaactg tacttttgga tctttttcaa aaaatttgcg gtatacaaca atattgcgat
     5401 gcacctgtct ttcttcctga tataaattat ccagtgaaga tccggttgta taaaccggat
     5461 ccatctcttt atttgctatg gtaaatgtca gattctgccc ttcatagctt ttcaatgttt
     5521 ccatgtcttc atcgccgatc gctgacagat ccatgcgttt tacttcttcg tatgcttttt
     5581 cgatcacccg gctcttctga tgccggtaat aagccggtgc aaacatatta taaagaagca
     5641 cacttcctcc gaccacgatc agaagaaaca tcagctccat caatagcata cttgccctaa
     5701 gcttctgctg tttcattttc ctctccttca aatatataac cgacaccata caccgaacgt
     5761 atataacttc catacggtgc aatcttcctg cgaagctgtt ttaccagagt atccaccgta
     5821 cgcgtatctc cgctgtaatc atatccccat accgattcca ggatgctgtc ccttgtcata
     5881 acgacatttt tatttttgat cagataactt aaaagttcat actctttcgg cgttgtctcg
     5941 atcgcctttt catccacata cactctccag gtatccggct ccaccgcgat gtgtccgact
     6001 tccaccctct ctttctgttt tccggcacgt ttcaggattg cctcaatatg aaccttgacg
     6061 atcgccagtg cataaggttt cgtgatataa tcatccgccc cgtttgtaaa gttcagaagc
     6121 tgatcgtgga tcgagtccct tgctgtcacc atgatcaccg gtgtattgga ttttttgcgg
     6181 atctccttaa ggatctcact gccatccata ccaggcagca tgatatccag caggatcaga
     6241 tccacttcat gcatatggtc ataaaacatc gacagccctt ccagtccgtt cagtgcccgc
     6301 agcacacggt atccgttcaa ctccagatat tctgcaagtg tcctggataa tttttcttca
     6361 tcttcgatca ataatatgac atttttgttc acggatatac tcccctttcc tgatttcaga
     6421 tttttccctg ttccccctgt tttcgtcatt ttgcatcttt tttctttttc gagtttacgc
     6481 aagaattgtg atataattgt gaaaaaaatg cttccatccg gtatcagcta tcggataaaa
     6541 gcacttttcg tccccatttt attatatcgt gtttaaaccg gatatccggt ccccacacct
     6601 gtttttgcta aactaaagaa taccttacag cacatatctc tcgtttttct cgatcacgat
     6661 cttgacgccg tcctcgccga catggtaaga attggcacgg atcgtatcac ggctctctac
     6721 gatacagttc tcgatgtgtg tgttgtctcc aagatagaca tcgttcaaaa taatcgaatt
     6781 cttgatcacg cagttttctc cgacgaaaac tttcttaaac agaacagaat tctccacata
     6841 accattgata atacagccgt ttgaaacaag gctgttcttc acttcacaac ccgggttgta
     6901 ttttgccggc ggattgtcat ctacttttga ataaatattc ggatactgat ggaagaaata
     6961 atcacgtact tccggtttca ggaaatccat atttgtttta taataagagt ctacggatgc
     7021 aatattgctc cagtaatctt tgatcttata tccatagata cgtttcagat ttttgtaacg
     7081 gatcaggatg tcttttacaa aatcgtggcg gtcttccagt gcacaccgct cgatcagctc
     7141 gatcagctgt cttcttcgaa tcacatagat accggtagaa atgcagttgg aattgcttac
     7201 catcggtttc tcttcgaatt cttcgatccg gctgtcttca ttcattttga taacaccgaa
     7261 tcttgttgcg tcttctcctc ttggaaggtc tttacatacc acggtgatat ctgctttttt
     7321 ctcgatatga tattccagca ctttgttgta atccagcttg tatacaccat caccggaagc
     7381 aatgatcaca tacggctcat ggcatttctt caggaaatcc agattctgtg cgatcgcatc
     7441 tgccgtaccg cggtaccaga aactgttgtc tgcagttgtg gtcggtgtaa atacaaacag
     7501 accgccctgt ttacgtccga aatcccacca tttggaagaa ctcagatgct catttaagga
     7561 tcttgcattg tactgtgtca gcactgcaac tttctggatg gaagaattcg tcatgttgct
     7621 cagtgcaaag tcgattgcac ggaaaccacc cgcgatcggc atggcagcaa tcgcacgctt
     7681 ctgggataac tctctcatcc ggtggttatt tccgcctgct aaaataatac ctactgctct
     7741 catgccatat caccttcctt tataatagat tcaccgctct ccaaaatacc atttggataa
     7801 tcttctgctg tggtaattcc tgaaatcgct gtattctttc ctatcttaac accggcaggg
     7861 atggcggatt tctcgccgat aacagccagt ccgaaggcat atacagcagg tttgaacttg
     7921 ttcggtgctt cttctccgat accgatctct acgtcattgc caaccgtaac accttctgca
     7981 ataatgcttc ggtctaccgt tacgttctcg ccgatcgtcg tgcctcgcat aataatagaa
     8041 tcacgcacaa ccgcaccttt tttgatcgta acgccaggtc cgatcacaga attatgtact
     8101 tcaccgtaaa tctcggtacc gtctccgacg atacatttat ccacttttgc atctgctgaa
     8161 atatactgcg gtgcaatgat ctcgctggat gtataaatct tccagaactc ttcgtaaaga
     8221 ttgaactccg gaatgatatc aatcagttcc atatttgctt cccagtaaga accaagtgtt
     8281 ccaacgtctt tccagtaacc gttgaactcg taggcagcca tgcggtctcc acgctctttg
     8341 agccatggga tgatgtgctt gccgaagtca cagttgctct ggtctttcag tgcgatcaaa
     8401 gattctctca atgctttcca tgagaaaata tagataccca tggatgcaag attgctcttt
     8461 ggctctgccg gtttttcctg gaaatctgta atgacgttgt tttcgtctgt gacaacgata
     8521 ccaaaacggc ttgcttcttc cataggaaca ggcataactg caatcgttac atctgcgttg
     8581 gttgctttat ggaaatcaag cattacttcg tagtccatct tataaatatg gtcacctgac
     8641 aggatcagga catactccgg attgtaagtt tccatataat tcaagttctg gtaaatggca
     8701 tttgctgtac ctgtgtacca ttctgtcttt gaactctttt cataaggcgg aagtacggta
     8761 acgccgccga cattgcggtc aagatcccac ggaataccga tcccgatatg agtgttcagt
     8821 ctcagcggct gatactgtgt caggactccg actgtgtcca caccggaatt gatacagtta
     8881 ctcaacggaa aatcaataat acgatatttg ccgccgaatg ctaccgccgg ctttgccact
     8941 tttgaagtca ggacaccgag acggcttccc tgtcctccgg caagcagcat ggctatcatt
     9001 tcttttttaa tcacgctatc acctcttaat attattttca catcaggaac cattttcccg
     9061 ttcccatgat gcctgaaatg catcatctat gttgtatttt ttattatatc acaggcttct
     9121 taaatagtga acatttttta caaatttttg aaaaaatttt cctgtttttt attataattg
     9181 cattatgtcg atttttctgt gaaaactctt tgaattatct gcctaaaaac acggtaaaag
     9241 ttatgccgga tttccaataa gctctttctt ttttccaaaa aaaagtttat actaaaatca
     9301 gactttaaca acaaaggaga ctacaactat gtatcagata gatttcaaga aaccaatgca
     9361 tatacatttc atcggcatcg gcggcatcag catgagcggg cttgcttcta tattattgaa
     9421 gcagcatttc aaagtatccg gctccgatgc ccacgaatcc gagctcacaa agcagctgga
     9481 agccgaagga gccattctct actacggtca gcgtgcttcc aacctggatg acacaccgga
     9541 tctggtggtc tacacagccg ccatccaccc ggacaatccg gaatatgccg ctgcggtagc
     9601 aaaacagatc ccgatgctct cccgtgccga actgctcggc cagatgatgc acaacttcaa
     9661 aacgccggtt gccatttccg gaacgcacgg caaaacgaca accacctcca tggcttccta
     9721 cattttcctg gaagcagata tggacccgac catctcggtc ggcggtatcc tcgatgcgat
     9781 cggcggcaat atccgtgtcg gcggccatga cacgtttctg accgaggcct gtgagtacac
     9841 gaacagtttt cttcatttct tcccgaagat cagcgtgatc ttaaatatcg atgcggatca
     9901 tctggatttc ttcaaggatc ttgatgatat ccgccattct ttccgcaaat ttgcccagct
     9961 gctgccggac gacggcacgt tgatcgtcaa cagtgagatt gaacacctgg atcagctgac
    10021 agaaggactg acctgcaaga tcgtaaccta cggtatggac gcttccagcg attattatgc
    10081 ttccaatatt acgtttgacg aattcgcaca tccgtccttt gactgctata aaggagatac
    10141 cctgctcggt cattttgtgc tgcacgttcc tggtatccac aatgtcagca atgctctggc
    10201 ttccatcgcc ctcgctgatc agatgggcgt ttccatggaa catacccgta tcggtcttga
    10261 gaaattcggc ggcaccaaac gtcgtttcga gaaaaaaggc gagatcggcg gcgtaacgat
    10321 tatcgatgac tatgctcacc atccgactga gatcgaagct acactccgcg ctgcacacaa
    10381 ctatccgcac aaaaagctgt ggtgcgtatt ccagccgcat acttatacaa ggacaaaagc
    10441 tctgatggat gactttgcaa aagctctgac ccttgccgac catgtcgttc tggctgatat
    10501 ctatgctgcc agagagacgg ataccctcgg catcagctca tccatgctgg cagagaaagt
    10561 gaacgctctt ggcggctgtg cggattattt cgactccttc gacaagattg agacctgtct
    10621 gctcgaaaac tgcaagccgg gtgatgtcct gatcaccatg ggtgccggcg atgttgtcaa
    10681 gatcggtgag aaactgctcg gaaaataaaa tctgcacacg gttttccggt cttgattcct
    10741 ttgatccaca tgtttctgca cattcttttt ttgcagaaca tgtggatttt tctgccctta
    10801 tttcccttat tctcaatgca tctcttttat gctatactag tggacagaaa tgtcggggaa
    10861 aagccttttt tcctcaaccg atgcctgcga attgctccac aggaggggag ttaccttatg
    10921 aaaaatatac gtttacatca tccgttttcc aacaacatga ctgccgtatc caactatttt
    10981 atcgattact atatgccaca ggccggcgga gaatacgtca agctctatct ctgtctgctg
    11041 cgtcatgcgg cggactatgg cagtattcct ctggaatcca ttgcggaact ttttgattat
    11101 accgagcggg acatctaccg cggcctgcag tactgggcag accagggact gatcgacctg
    11161 gcatttgacc aggacggcag catcagcgat ctggtctttc tggaagttcg aaagccggag
    11221 agcgtgcagc aggctgctgc ttctgtctct tccggagtgc tccctgcatc tgtaaaggca
    11281 cccgtttcca aaaatacaac aaaaaaagtg gcttccgaac cgaatgattc cacggaagca
    11341 aaggtccttt ctccaaaatt gaagatttcc agagaccgta tggctgtcct tcagggagaa
    11401 caggatatca agcaactcct gctgatcgca gaatcttacc tcggtcacag tctttcgccg
    11461 actgaaatgt cttctatttt atattattat gactcgctgc attttaattt tgagctgatc
    11521 gaatatctga tcgagtactg tatttcacgg ggaacgaaaa gttttcatta catagataaa
    11581 gtagctctgg catgggcaga ggaaggcatc acaaatgccg ggcaggcaaa ggaacgccag
    11641 tctcagtaca gccagaaata ttattctgtg ttaaatgctt tcggcatccg cgaccggggt
    11701 cccggcaaac cggaagtgca gatgatcgac cactggtttg atgacctgca ttttacttcg
    11761 gatatcattc tagaggcgtg cagccgcaca ctgacccaga cgcagaaccc gagtttccag
    11821 tatgccaaca aaatcctgga aagctggcac gcaagcggta tccaccatct gaatgaaatc
    11881 cgggaacttg accagaaaca tcagaagacg ggaaaagcgg catcgaaaca gcgtcctgcc
    11941 gtgacttccg gaagcaaatt caacaatttc tcgcagcgtg actatgattt tgatcatctg
    12001 gaatcgctgc tgaaccagta ggaggactgc catggcactg acgaacacac aatacgatac
    12061 gatcatccgc gaatacaaca aaaaacagct tcagaaccgc cgggatcagg aggcacgcat
    12121 cgcccttgca gaaaaggaac tgccggagct ttcacagatc aatggagaaa tcgcttcttt
    12181 aagcatgaaa tgtgcccgcc gccttctgga aggggaggct gcttcgatcg atgacctgaa
    12241 aacagagatt gccgcatacg gtatccaccg caaagctttg ctgaaaaatg ccggattccc
    12301 ggaagatttt ctggaaatgc attattcctg cccggactg
//
LOCUS       DAWDWH010000037        12112 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_2824_length_12112_cov_18.990296, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000037 DAWDWH010000000
VERSION     DAWDWH010000037.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 12112)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12112)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12112
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2824_length_12112_cov_18.990296"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7403..7412
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3057 a         3189 c         2023 g         3833 t
ORIGIN      
        1 ggtctttcat ccacctatga gcaagctcat gtggatttag accttttgac cctggaatca
       61 ttaaatgatg ttgaaatatt tgaccgaacc ggtctgatta aatgatttta gaaattcaca
      121 tctatgatac atcaaatgtg aactggctcc cgcctcgaca gacggcaagc aacaagtttg
      181 tatcaatggt gggattcgat ctcccaaccg atgaatgtat tatatatctt ttgtatttgg
      241 atgtctatag taagtaacta tattttttaa ctttgtgaga tttttacaaa acctttttac
      301 aaaaactgac acccctcaat tccatacaat aaaagcaccc ccggaacgcc cagaactccg
      361 gttgttgtca cgctaatcac attgcagcca accttaagat ccactcctgc attgtcaaaa
      421 acctgattta agaaaaaaac acatgccatc ccaataatta tccgtaccag aaaacgagaa
      481 atatacttca taaaagcacc ctgtcctttt tctaaattgt atgaactcta ctgacaattc
      541 agtacagatt gcagcaagaa agtcctgaat ataaaaagta ttatacttta ctccgatatt
      601 cttatgatat gaaaagatgc cagacaaatt gctttgtact ttttacttcc acaattctct
      661 ggcaccttct tattttttca gatcagaatt taaagattgc aattccgtat gccggcagat
      721 cgtatgcaaa agagtattct cttccgtcac attcactctt cactgccttg tatacaaccg
      781 gacgctccgt tgtatttccg ccaaattctg ctgcatctcc atccagcacg aggcgatact
      841 gttttctttt tggtacaccg acgcgataat ccggtcttgc taccggtgta aaattgatca
      901 caaagagcag attgctctta ccgtctttgg aatgacgcac aaagctgaaa atgcttcggt
      961 cggcatcatc tgcattgatc cattcaaatc cgcatggatc ctgatctgca tcataaagtg
     1021 ccggtgtctt tttatagaga tgaagcagtg ctttgacgta attctgcatc tgctgatgtt
     1081 tgtcttctgc aagcagatac cagtcaagtt ctcttgcttc gctccactcc tgaagctgtg
     1141 caaactcctg tcccataaac aacagttttt tgcccggatg accaagcatg tatgcatagc
     1201 cggcacgtaa gtttgcaaac ttatcatcgt agagtcccgg catcttattg atcatcgaac
     1261 atttcagatg aactacttcg tcatgtgaga gtaccagtat gtatttttct gcataagcat
     1321 acgtcatgga gaacgtcatt ttattatgat tccatttgcg gaaatacgga tctaatttca
     1381 tatattccag gaaatcgttc atccatccca tgttccattt atgggagaat ccaagtccgt
     1441 cctcttccgg ttttccggta actttcggcc atgctgtgga ttcctctgcg atcatcattg
     1501 ctccatgatt tctgcccagc acaacgctgt tcagatgttt gaaaaactcg atcgcttcca
     1561 gatttttatt tccgccgtaa atattcgggg tccactgacc gttttctctt ccataatcca
     1621 gataaagaat ggatgccacc gcatcaacac gcagtccgtc gatatggaaa tgctcaaccc
     1681 agtaaagtgc attggcgatc aggaaattct tgacttcact cttgccaaaa tcaaagactt
     1741 ttgttcccca ttccggatgc tcgccctttt ttggatccgc atactcgtaa acacatccac
     1801 cgtcaaattc ttccagtccg tgtgcatctc gcggaaaatg tgccggtacc cagtcaagga
     1861 taacaccgat tttctgttta tgcagataat ttaccatata agcaaaatct tccggcgtac
     1921 catatctgga agtcggtgca taatatccgg ttacctgata accccaggag ccatcaaacg
     1981 gatgctccgc aatccccatc agctctacat gcgtataacc catttccttc acatattctg
     2041 caagtgattt tgcaaactct ctgtaattat agaatccatc ctcgtcctct ccatgcggat
     2101 gtttcttcca ggaacccgga tgtacttcat aaatagaaac cggaaggctg tcattatctc
     2161 tctgctttct tgcttccatc cacgccgcat cactccatct gagtttcgtg atatctgtga
     2221 tcctggatgc ggttcccgga cgctgctctg cataatttgc aaatggatcg gctttgtaaa
     2281 gtcctctgcc atcttttgtt ttgatgaaga atttatagag catacccact tttgctccga
     2341 ctacaaaacc ttcatagata ccggacggct caaggcgtgt catcggattt gcctcttctc
     2401 tccagccgtt aaattcaccg atcacgctga cagtagctgc attcggtgcc cagactgcaa
     2461 aataaacacc ttttttgcca ttcatcactg cttcatgtgc acccattttt ttataaatat
     2521 cataatgcgt tcccacactg aacagatact gatccagttc tccgatacgg aaatcttcct
     2581 ttgctgcagc ctttgttctc gttgttcttt tcgctgctgt ttttgctgtt gtttttgttg
     2641 ctgttctctt tgttgtattt gctgccgttt tcccggctgt ctttcttacc tttctttctg
     2701 ccataactac ctccgacttt ttctatacat ttcaaccata aattgatatc agaatcacaa
     2761 atgtcatact aatccttttg aactggcatc atttttttct gaatacttta ctttcacatg
     2821 catccagatg aacctggagt ctgccatcca ccaccggcac tgcttctttt gtgaacgcat
     2881 tttcataagt tccgtccact ggaatcggga tgctcatatc cgccggattc tcatcgttat
     2941 tcgctgccac aacgatggca tcctgatctt tgattcttgc ataagcatac tgtctgtttg
     3001 tcagtaccag ctctctgtat tttccatctg ccaaaaccgg attttctttt ctcatctttc
     3061 caagtgccgc aatatgtgct gtcagctctg gatgcggatt ctctttctgc attttttcaa
     3121 ggtcaagctg cggacgcatg gcatcatctg aggcaccctg ctttcttcct tcaatgcccc
     3181 actcggagcc ataataaata gatggaatgc ccggcagcgt gtagagcagt gtataaacct
     3241 cgcggatatg gtctttgttg tttaacttgc tcatcagacg gtccacatcg tggttatcta
     3301 caaaagaata caaagtactt cctctgtaaa taccgccgtt gtcatcaaac tgacggcgga
     3361 tcgtatgggc gatctcaaaa taattatgat cattgtgtcc cgaatacaga cctttgtgaa
     3421 gctcataatt cgttgtggaa tgaagcattt cgtcatttac ccatctcgca taatcgccgt
     3481 ggatcacttc acccatcagc cagaaatcct ctttccactg accggtgcgg taacgcattt
     3541 ctttcataaa atcaaacgtc agacagtcag cacagtccag acggataccg tcaatgtcaa
     3601 actccgtgat ccagaaatga atcacatcaa acagataatc acggactgcc tgctcgtaaa
     3661 gatttaaatt gaccagctca aaacagtttc tccatgcttc ataaccaaat ccatcgttat
     3721 acggagaatt ccagccaaaa ttcactcctt tataccaact gcagtacgga gaatgttctc
     3781 tgtttttctg gatatcctga aatgcaaaaa actctcttcc ggtatgattg aaaacaccgt
     3841 ccacaaccac gcggatccca ttttcatgtg ccagtctgac aaattctttg aaatcttcat
     3901 tatccccaag tcttctgtct acttttttgt aatcttttgt atcataacca tgtgtagaag
     3961 attcaaacag cggtccgata tagatcgctg taactccgat ctcctttaaa tggtcgatcc
     4021 acggaaacag ctcccgcaag cgatgcacgg tctccggttc tttgttttcc tttggtgccc
     4081 ccgtcatacc cagcggatac atatgataaa aaactgcttc gttataccac tttcccattt
     4141 gcaaatacct ccatcttaac tgtaaatacc gtacataaac tgctttacaa gtgcccgctt
     4201 tttctcctct gaaatctgct gcggtctgcc ttcccggtcg catgccagca gaatataatg
     4261 attgatcaga ccgataaatc caatcgcaaa ctgctcttgc cttccgttca tatttccaag
     4321 ctcatgctcc gccgcatcaa agaaatgtac gatctgcgaa tagaaccttt tcacaagcgg
     4381 ctttaccgcc cggtacgctt cattctcctt cgcagaataa aacatcgcca tcagaagtgc
     4441 atagacttta taatttctca tcgcataatc taaaaatgca gaagcaatat tgtaaagcgt
     4501 cgctccgaca tccccttcat aaaaagatgc ccgctccagt tcttcattca gaacagtaaa
     4561 atgtttcgtc agcaaagtct ccagaagacc atatttgctg ccaaaatagt agtacaacgt
     4621 aggttttgta atccccgctt tctgagcaat ctcctgtacg ccaacggcat cgtaaccctt
     4681 ggcataaaac agctccagtg cacactgcaa aatacgttct ctattgtcca taaatcctgt
     4741 cctttactcc tgtttacggt tgctttcttc attataccgt tcggtatata aagtgtcaag
     4801 gaatatcccg ctaacttcat aataagacag tccggtttcc cggcagattc ttgcgacttc
     4861 ttcgtattcc ggataaatcc tttttccatc cggcagatca catactttta ctttcatttt
     4921 cccaaacggc gtgtcgatct ctgccgcttc tctttttaag atacttcgtt ccatgtacat
     4981 tctgcggatg ccaatcgttg tcgtttcctg aaaaataagt ttctccagct tttccgcatc
     5041 ttcttttttg cagattacat ttacctgata tgccgggcga ttttttttca tataaaccgg
     5101 cgtataatga acatccttcg cccccacctg catcagacgc tccatcagaa atccgagtgc
     5161 ctcgccgctg cagtcatcaa tatttgtctc cagtttacag atctcttctt tttccatcca
     5221 gctcttttca ttctcgcttt ccagaatcat tgcacgcaga ataccggcac aggcatattc
     5281 tcgttttcct gcaccaagac ctgttttttc gatgaaaaat gactccggaa gtttatcaga
     5341 agtacggatc gccgccacga tcgctgcacc agtcggcgta accagctcac cttctatctg
     5401 cgtaagtttc agtttcagat gattggcctg tacgatattt gtcaccgccg gaaccgggat
     5461 cggcagaatg ccgtgctgac agcggaccgt acctcttcct tcgcaaagtt ctgtcacaat
     5521 tacctgatcg atatccagat tatccaggca gaccgccacc gagagaatat ctacgattga
     5581 atctacagca ccgacttcat gaaaatacac ctgatttctt ggcacgccat gtgcttttgc
     5641 ttctgcatcc gccagaaccg aaaaaatatc cgtcgctgtt tttttcgcac gttccgtcag
     5701 gctgctgctc tcaataatct gcagaatatc cacaaaactt ctgtgctcat gtatatactc
     5761 tgcatcatgg ttatgcacat catgcccatg catatgctcc gtagcatgat tatgtgcata
     5821 agtatgacca tgcagatatt ccatatcatg atcatgattt tcatgttcct tatcaagaac
     5881 cacatcaaag tcgcacgcat ccagaccgga tttttttacc ctggaaatgc ggatatcaaa
     5941 tccctgtacc gaaaggcttg caagagcttt tttcagaacc tgctgatctg ctccaagatc
     6001 cagaagtgct gccaccgtca tatctccgct gataccgctt tcacactcca gatataacgt
     6061 tttacccatt cgccttcacc cccagtctgt tgatctgcgt tgccatataa cctgctccgt
     6121 aaccattatc aatatttacc accgcaattc cgtttgcaca ggaatttatc atcgtcagaa
     6181 gtgcagaaag tccgccaaga tttgcaccat aaccgacgga agtcggcaca gcgatcaccg
     6241 gcttgtccac cagcccgcca agtacgcttg ccagtgcacc ttccataccc gcaacggcaa
     6301 caacacagtt tgcactctga attgcctcca gatttctaag cagtctgtgg atgccgctca
     6361 cgcccacgtc ataaaccctc gtcactttcg ttccaaaata ttctgccgtc tgtgcagcct
     6421 cttccgctac ggcaatatct gccgttcccg cagtgcagac tgcgatattt ccgatatgct
     6481 ctttatcctt tttttcaatt tttatgatat gggaaatcgg atcatattct acctgcggat
     6541 atttttcacg caatatttcg tactgatgct gtgttgcccg cgttccgaaa acctgtccat
     6601 ctgccttgta taaatgctca aaaatatgaa gcagatgttc atctgctttt ccggcacagt
     6661 agatcacttc cgcaaaaccg gatcgcacct tgcgatgcat atccagtttc gcatagccca
     6721 tctcttcaaa cggctccctg cggagaaatc cttccgcttc ttccacagac atcgccccgt
     6781 tttttacttt ttccagaatt tcctgtattt ccacaaaaac acaccccgct ttatactgtc
     6841 ttctcaagac acacaagctg cacgcccgga atgccgttaa acacgcaagg cacaatatcc
     6901 gcctcccagt atccaagccg tgcatacaac tttctcgcag cctggttttt cgcattcgta
     6961 tccatacgca gatacggaca gttatgttcc tttgcataat cctcataaaa cttcacaaac
     7021 gcacttccat atccttttcc ggactgcccc ggatctacga ccaacgtatg cagaaccata
     7081 acctgttccg gcaaaatatc ctgatatttc cagtttgcat cagcatactc cgacacctga
     7141 acctggttga tcctcgctgc tgccacaatc cttccggcat ccgtcataac aaacagatcg
     7201 tcatttttca ccgcttcttt tgcggtattt tccgtcggat aaacgccgcg tatccagccg
     7261 atcaccgctc tgccttcctc ttcctccgtt aaaatatgat cataaattgc tgcaacctgt
     7321 gcaatatctt cttttgttcc tctgcgaatt tccaacattt tctgtcctct ctgtctactc
     7381 agaaaccaaa atcttcataa tcnnnnnnnn nngatgataa ataatatggc atccttcatc
     7441 tttaaaacaa taataatatg gatcgtccgg tttatcttca aaacggatca gaatatcaaa
     7501 ttctccattg tccatatttt ctgtaataat attatcagaa tacttatgaa acaaattcag
     7561 aatatcatcc atcttgcatc catgtttctg aagcaactcg atcaggcttt taataaattc
     7621 attttccttc atattattat ggacgtactc gctgccttcc tccggaatgt ggaagcaaaa
     7681 tcgctccgct ttgtgtgtcg cagcaacctc tcccagcttc tctgtgatca ccaccggaag
     7741 gtggttcaga cgctcctgca tctgatctgc atcatcactc gtaccaaaaa aatgattcaa
     7801 agagctgagc ggataataca ggcgaatttt ttcttttcga aatcccagtt tcgcatggtc
     7861 ttccttgatc acatcaatca gattctttaa cagttttttg tatccttcat ttcccggaaa
     7921 ctgatacaga ttcaatccaa caccgtcctt atcataaaaa cgttccactt ttccattgac
     7981 tgtgcggatt gtgatttcag ccgtcgccga aatccgacac tcattgagat gaccgccttt
     8041 gacattcatc ttctcatcgc agagattaat gtgaatatga agataagttt taccatccat
     8101 cgtggaaaca tttcccgtca gatccgtgat ctccatcggt ccgttcagtt ccgttttatg
     8161 gtaaacctgt tccccgacat tatacagacc gatcaccgca tggtctgccg caccgatacc
     8221 ctcgatcgtt gccagtgtga tcttttccct gtcgcaaatc tttgtcagag actctacgat
     8281 ctcctcgcca cggtccagac gaacaacata tgcatcatca aatttcatgt attccatatc
     8341 tatatcagcc tttccttttt ttgtccccat aataccacga aagaagcagg gattgcaaat
     8401 ttttataatc catttcatcc acttacataa aaatgatgtg gatgtcaaaa gtccccaact
     8461 atgatgccta cggattgttc cgtgcgttgc actcccacaa tcctggcatc ataaaaatac
     8521 agaagattat gaaatttcgt tttcataatc tcctgcatct tctctttata cttctattcg
     8581 aatttgatta taatgtgcag cctaccggat cttaatcgtt acagatttct tctttccgct
     8641 tccatccgtt gccattgcgg tgatcttcac tttctttcct tttgcatttt tctttgcctt
     8701 tacttttccg tttttcaccg tagcaaattt ttgattgctg cttttccaaa aaagtctggt
     8761 attggccttt tttgtggctt ttactttcgc tttcaactta atgctctttc ctgctttgac
     8821 ggttttcttt ccggaaatcg taactttttt cacaacacct ttcataccgg tgattgtata
     8881 aactgccttt ttgccgcttc catctgctgc cttcgcagtg atcgttactt tctttccgcc
     8941 gctgcctttt ttcattgtga caataccatt tgcatttacg gtggcagctt tcggattgct
     9001 ggaagtccat accagtgttt tgtttgtcgc atttgccggc gaaatatttg ccttcagtgc
     9061 aattttcttt cctgctgcaa tcttatttga aattccgctg atcttaatgc tgccaatcag
     9121 tatactcttt gaagaatctg tacccggtct tggatttgga ctcggatctg gttcctgatc
     9181 ttggctgccc ggatttacaa tataaaatgt actactcaat aatccttcat aatcaccaat
     9241 tccatgaaca ccggcccagg cttcgccaat ttctacatta tcatccagaa aaacttcgta
     9301 atccacacct tctttcaaaa tttttccatc tgcatcttct actgaaactc tcggaatcac
     9361 cgcatttcca gtataacgaa gatcgccgaa tacatacatt cttccatctt cgatattttt
     9421 ctttgtgttc ttctttttgt agataaatgt ccaggaatct tcgtcacctg ccgttaccgt
     9481 catctgtgct ttttccggtg tataaccgct cacatccggt gcatccagcg tctgggaact
     9541 tccaaacaaa gcacggactg ttgatgcttc tgccagatca tctcccgctt catctttgta
     9601 atatactgta tagtcttttt cttccggtgt gattcctgct tcataggttg tttctgcaga
     9661 gtcatactgt actgcaaccg tcgcactttc agttgttttt tctttaaaat aagcataata
     9721 gccagatgta acttcttcac ttgttccatc atcccaggta atattcagcg taagtccggt
     9781 cgtatcgatt tctttctctg aagagccata ctgctgttta tctgccggtg ctgtcacttc
     9841 cacagaaact gccctgcgga tacgttcctc tgccatgatg ccatttgttt ctgcatactg
     9901 ccatgccgta ccgttttcac tgcaccataa tgtcagattt gcattatctc caaatgcgga
     9961 atctccaatc tctgtaacac tgtccggcac ctctacacct gtcaaagaat cacagcctgc
    10021 aaatgcatct gaaccgattg tttccagccc atttcctgat acaaccatat caagtgaact
    10081 gcagttttca aatgcctgac tgccgatttc cgtcaccgta tccggcagga atacagcgtt
    10141 gattttatcc tcattccgga atgcctgcgg atcgataccc acaacggtac gtccattgat
    10201 cgtctccgga atgcagagca gatcctgtgt atcactcagt gcccttgtca tacgtctgga
    10261 agcatctttt gtcttttttc catttaagct cacgatccat acgtgattgt tttcatcggt
    10321 gcgatactcg aatttcgccg cttctgttcg gttcatattt cttctgcagg ttgcgataaa
    10381 attgtcatac cgcattttct gtgaatacag tctgatacac tgatccagcc attttttctt
    10441 atattttaca gctgcatttt cccctgattt ttccgcaagc tcactgcact tatttgccat
    10501 cagaagaagc gaagcttcat atgcttcata cgcaaactga tattctttgc gaagttcttc
    10561 acctgctgtt tttccggtct tctgataatt ttgtgcgatc tcagaatact tatcattcag
    10621 tccggcactc aatgaattac agagcgacat actgtaagca atctgcacca gctcatcata
    10681 gtctgcaagc tttatttgtt tgctgatacg tgaatagaac agttttccga ctgtaagtgc
    10741 accgccgaca tcaatgccgg aaagcggatc aaacaacttt gccatgtctg taactccctt
    10801 tttcacaagg gcttccgcaa tttcttcaaa gtacttatct acaataaatt cctgaatcgc
    10861 tacttccgat gatccccagt atttctgcaa ttttccctgt aatttcttca atcctttata
    10921 agctgcggaa tccggcatta cgctgttcag aagctgattg accgtttcca tttccagatt
    10981 cgccataata cagattgttg tgccaacctg aactgccgaa gttacaattc cgactttttt
    11041 aaaaataccg tcaatttttg accagtttcc ttttacctgt tcaaaaatag cttttgcctg
    11101 ctcggtggta agtgcgtctc cttttgccag tttggccagt tcccactgta cgctggtatc
    11161 cattggcttc tttgccgctg taacgagctt ataaaattta gaaacatctt ttactttacc
    11221 gcaaacgtta tccactacat tttttaatgt atcatcctgt tcatcaatct gacggatcag
    11281 ttcaatcgcc acttcctctt ctaacttatc ctcttcccat cgttttccgg taattgcttc
    11341 cagaatctgt ttcgtcggac gcacgatcac atcaccggac aatgtcttaa taacagaaaa
    11401 cagctcatca ttatccgtac gtgccgccag aacatccgca gccgcatcat acagcgttct
    11461 gtgcatcgtt ttgtatgcct ggttatctct cagatagtgg tgatattttc caaacagatc
    11521 atccagactg tatggatttt gtgcattatc ctcagatgat tctgaaaaat catacagata
    11581 acagccatcg tcctctttca tcatcacaaa accagactcc acataggtcc attcgaaatc
    11641 gtaattccct gacaacagct cttttccggt ttgatcaata attttacagt tgctgtacca
    11701 gcttcccact tttgcaattc cgttatgcat atttccggca agattaaact gctccgcaat
    11761 ctgcaggttt ccgtctttat cacagtaacc ccaatcgccg gattctcctg ttcgaacccg
    11821 aatcaaccca tccgaaccat aacctgaacc atccacatat tttttcagca aaactccctt
    11881 acgactgtaa accagatact ggtcatagtt cacagaatca ctgatccaga aagcaaaatt
    11941 tccatttttt gtaaccgata cattacttct tctgtaaaac tcattcatct gtgcgatctt
    12001 ttgaaaagaa ccattataga atacaccgtt tatcaaaaat acaatatccg aaccaacaat
    12061 actttctact acaatatatt tatggttaat atcccttaca gccagtaagg aa
//
LOCUS       DAWDWH010000038        11938 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_2866_length_11938_cov_12.650088, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000038 DAWDWH010000000
VERSION     DAWDWH010000038.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 11938)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11938)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11938
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2866_length_11938_cov_12.650088"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2406..2415
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2953 a         3054 c         2205 g         3716 t
ORIGIN      
        1 tctgccatcg gtgtgctgta tgttttcaca cgttctgcac cgttttctcc agacattgcc
       61 tctgcgatat ttacacgctc tgcctttgaa agatttttaa atgcttccag tgtatccagg
      121 ttgttccagg taatcatagt agattcctcc ttaaatgtat aaaatagtat aagcgggtgt
      181 gtgataaagt ttacttctct aaatcactga tgattataat agcaaacctg cataaattca
      241 agcatcaatc gcatctccat ggttttttac accgtaagaa ttgtttttta ccaccattta
      301 atctcctgcc gtttcattaa atttactaaa cagttggaga tacattgcca ttttatccac
      361 tgcatccaca gatacacccg tttcggtatc aatttcgaaa tagcatttct ccccaatata
      421 acccttgagt tctgcttttc cgctgccgat ctgattgccc tcgtaatcgt aaagttcctc
      481 tccgcgttct tctgccagat aatctttgag atttccgtct gcatccataa atacgagata
      541 atattcacgt tccagatttt cgttctcgaa caaccgcttc tgtgcatagc caaggcactg
      601 attgttttca tcataataac cataggttgt cccggcaatc acgccatcga tgttattgat
      661 cggctcatca gtcacaaatt tcatccagaa ccaggttttt ccatcgaccg ataccgtcca
      721 tttcgtattg agaatacctg tttcttttaa ccgcccggct attttcccgt ccgcaaggat
      781 attcccctcc tggttgacct catccagatc tccttctacc agaaatgtcc ggcactgact
      841 cattgcctgc tttgcttcct ctgcagatgc ataggactcg acattagatt ttctgcatcc
      901 caaaagaaca cttgccagaa tacaccacat cactgcgata tacaccgctt ttcttatctt
      961 tgctttttct ttcattcaca cctcctaact gcgatcccgt gcaagttcgc gtgccaccat
     1021 caccgccatt gccgcaagca gcggaagata tttttcttca tatgcaatga tcactgcatt
     1081 gtcaaaaacg atattttcca ttttcagatc caggttgtcc aggattgttc ccagcgtttt
     1141 gccatttaag gttacgcaga aattatggcc gatcgaaccg gcatattcgg tcagttccag
     1201 ttttccttct ttgaccttca tctcaaattt atctttgacc agcacaaatt tcttctccag
     1261 cgtgccgtaa agctcaccca ggtaataaaa ctggtaggtc ggcagtacac ttacgatctt
     1321 cttggtcatt ttaaagatct cgttatgctg atcgtcataa acagaaagtg tgcgcagcgg
     1381 agccgttcct tcgatttcga aaacacgatt tccttcgttg tcacagagat caaaatgctg
     1441 accggtcagt ttaaaatcct ccgtaaaagt atagacttcc gatgggtggg actgcggaat
     1501 ttccaaaacc tgttttgttt tcacaaaatg ctccaggctc tccctcagtt gcttctgcat
     1561 cagaagcaca tccgactgat agcagacata ttttgctgta ttatcgccca aatcctcttc
     1621 tgccggaaat atcggatcat tatattttcc ctttatcata ctggtcatga acgaatagcc
     1681 aagataaccg atattcccgt cttttgaaag ccagaaatag agataatcgc cgtttttgtc
     1741 cggcccgaac atcagagccg gctgtctcat cgtgtaaaac tgaacaagat aaccggagcc
     1801 aagcggcgta cggacatctt tgtaggcctc ataaaattct tcaatctttc ttggacaatc
     1861 catggcgatc atgctgcaaa tatctgttga accattgtaa tcatccggcg atgccagcat
     1921 ccagatcgca acaccgatga tcagtacaac cgttgccaca ctgagcggaa tcttattcca
     1981 gttgcctttt gatgcgatcc agacggcaag tgcacaaact atcacactga tcatacggta
     2041 cttccacata cgtgctctaa gtggagcgga aagtttctgt ttttctgtat tttctgctcc
     2101 ctgcatagtt ttcatccttt ccgagcattt gcgagtgcca aaaccacctt tgacactcac
     2161 tgccccggta ttttttatct tatgcttttt taagttttac tttgattgcc tgcacttttt
     2221 cttttttcgt gactccgcgt gctttcaaaa ttttcttata agcagactgt tttttcttcg
     2281 gaactttgat gactgctttc gcatggatgc cggaaaaagc cttgcttccc acgtttttct
     2341 ttgtgagctt cgtcgttttg atcgtgatat tcttcaggtt cttacagcca taaaatgctg
     2401 tcttgnnnnn nnnnnaatca ctactttttt aatctttttc tggtttttaa atgcatttgc
     2461 ggcgatcgcg gtaactttat aagtaacatg atttacggta acactgcccg gtatttttac
     2521 cgtacctttg gttccctttt tcggcttcag aaaagctact gtaaaagttt ttccatctgc
     2581 acaggttact ttatactgat ttttgtccgg tccggtgaac tttcttcctt ttttcaggta
     2641 ttctgttttt gatggcttct gtatcggctc agaagttccc ggttttgtgc ctggatctga
     2701 cggtttttgt ggtgtctccg gtgtttcctt tgtctccggt gtctcgatcg tcactgcttt
     2761 tgtcagaggg atttctgtga ctggctgtac cagcctgtaa tttgctttgt cttttccggc
     2821 aagcgtcagt tccggtaatg ttaccgaagt atattctcct gcttttttat cttttaacgt
     2881 tccatgcaaa tccgtcagat ctacgctcac ttcatcgcct tccagcatac cacttagcag
     2941 tacttccgtg atggcaacct gatttgtgcc atcatatttc ctgtttctgg cggtcgcctt
     3001 tgttaccgta agttcttttg gatttacttt gatcgccgtg ctgccttcta catttgcata
     3061 cgctacatga tccggtgtaa agatccattc tgcacttgtc atggatgtat ccggcctttg
     3121 tgtggcatct ttccacgcaa gcgttccttc cactgtattt ccttctccgt ccacaaattc
     3181 tgcttccgaa aactccagtg ccgacaaaag accaccgtaa ataagcgtat cttttttcag
     3241 ggttacttct gttccttcct gtaatctgac cggtatccgg tcattgcatt ccagattgaa
     3301 ggtgatcaca aagtcctcat agttctcagt cgtgactttg atctggatct ttcctttcct
     3361 gtccagtgcc ccctgcttca ctgtataaga taatatatca tttgctatac tcggttctgt
     3421 gatatattcc atgtttccgc tcatctccgg tgcagcatac tgtgcattcc cgcagtccac
     3481 cggtaggatt tccctgagac tgatccgctc ttcctgatcc tttaaatata agtaactttt
     3541 attctgttct tcgatctgaa gcccactgct ttttgccaca tttaccgctt ttgtcagagg
     3601 aatttcgtct gtcggctgca caagaatata attctccttg tcttttccgg ttaatctcag
     3661 ctctggcaaa gttaccgaag tataatctcc cacttttaca tctttcaaag tcccgcggat
     3721 cgttccgatg tctgccgaaa cttcatctcc ttcttcaatg ccctcaagaa caatatcacg
     3781 gaagctgaca tacgcggttc cgtcatatac tttatcatct gccgttgccg ctaagacttt
     3841 cagttctttt ggctctacgg taatcgctgt gctgccttct gcctttgcat atgccacatc
     3901 atccggcgtg aagatccatt ctgcacttgt cacggatgta tccggtcttt gtgttccgtc
     3961 tttccatgca agcgttccgg gaatctgcgt atccgttccc tgctcaacaa agactgcttt
     4021 ttctttccct tccagattca gctgcagatt ggataatgtt tctccgtaag taagtttccg
     4081 atcctgtaaa acttttattt cactgcctgc tttcagaaca acctttttct gatctgtcag
     4141 tgtgaccgtt aagtcataag aaacatcctt ataatttttc attgttaccc ttacatgaat
     4201 tgtgcctgtt tttcctatca tgctcctgtc tgtaaaatct ttcactttat aatttaactg
     4261 tccattccgc gtaatctcat aatcatcaaa aatctgctca tcccctgctg cagaatagga
     4321 ataataatct tcctttttgt ccgccgggag ttttgcccgc agatccacaa ataccgtgcc
     4381 agaacccttg gtataaatat aacttgccga ttcatgaaat gcagtctgct gcggagtctt
     4441 tttctcaaca tgaacttcta caccttttac tacataataa ggataatttc catctttcgg
     4501 tgtaaaaaca agatcgcata tcaatctttt tccaagttcc ggtatcactt ccagattttc
     4561 tttctccgta aaactccagg ttccttccag cagcgtactt tctccgtttc cggtatctgc
     4621 cctgatcacg cccggaatta aggtatactc ctgcaaagtc tgttctacat ttccggaaat
     4681 ctgcggaagt gtctcacagc tcatacctgt atttcgaacc tccacatttt tcagatcact
     4741 ctgagtaagt acttcttttc tgctcagaaa gcgtccatct tccgtactgt agaacgcata
     4801 accctgcgga agcatatttt ctgcactgcc tgaatataat acgatgttct gaaaatttcc
     4861 gccggtcatt tctatactgt tttgataact gtcccaatag ccgatctgtc tgaaatcacc
     4921 gctttctatt ctcagtttcc cgctcctctg tgctattaca atatcaaagg ttccgttttt
     4981 taccgtaacc tctgctccgt ctatgtagat ccggtcaaca cttttcgctg tcagagattt
     5041 ttgatttaaa tccagagtga tttttccact tttcagagtc ggaagacttc ccgtattttc
     5101 cattgactgc ataaaggtaa tctctgcttc tttgccattt gcgtattgta acgcttcttt
     5161 cagtgtatct acataagttt ctactccatt tacggtgatc cgaacataac cctggaaatc
     5221 cggatttttc ttatggcacc acacgcagta ctgcacatcc tgctcataat catgtttctt
     5281 acacgcctcg acatgcacat tcgtacagct tgtctcggca tatggctgtt tctcattcgt
     5341 ctcattatcc accagacaaa gctcttctcc aagttctgcc cattcttttg aaacccgatc
     5401 gaattttcca ccgctcacct taattttcgc accgttatac acaaaatagc tgttcgactg
     5461 attttcataa attccgccgg caaactctat atttttactt cttactagca acgtaccgta
     5521 aaatacaccc gtctgcgttt cactgctgtc tgtaatttta agagaagcat cctgattcag
     5581 agtggtcctc tttaaatttt gcagacgata tccattcaga tccagaatca gatttccact
     5641 gtttacctcc acttcccgta ccgattcaat attcttcaac agcttcatct gtgctgtttt
     5701 tccctctgta tcacgaaatg cttctgtcaa agtaacatat ggttcggaat gtccatccat
     5761 agttacctca atttctccgt ccgaaatttt atataacaga taatccgatt ctgatttata
     5821 tttttcacgg aaagtcattt ctacttttac atagtaatcg cctttatcag ccggcgctcc
     5881 gccttcggta agtgcaccgg cattttctgc atttgtcagc acgctgccgc gatctttata
     5941 ataagtggtt tttacggatg tcggtgtttc ctcgcagctc caggtgattt ttcttccttt
     6001 ttcaaaggac agttcccgtg gatttccggt ataagcataa tctatgcttt tgctgcctgc
     6061 gattttttcc atctggattt cggcatcgaa gttgatctga taggaaaaga tttctgaagt
     6121 ttcctgataa aaatcttcca cttccagagc cgtcactttt acataatatt gtcccggcag
     6181 tgccggtgct ccgccttttt tagcagctcc gctgttttcc gccgtggtcg gtatctgtgc
     6241 cttttcatcc agataatagc cgacctctat tgctccgtcc cctttccagt taaaaacctc
     6301 gttttttttc agagacagtg gctttgtttc agacagatta tcgcttgtcc gctcaatctg
     6361 ggtaccggat accagtgaaa tctcattttt tgccatactt cccggatgat tgctgtaagt
     6421 atcgttttcc tccagataat aaacctttcc atgtgtgcca tccattacag gataagcatc
     6481 cggattttct tcaagattct ggaaccactt tgcctgtgaa gccagagaat tatcctttcc
     6541 atttaacacc caggtcgcct ttccggatgc aaactctgcg gttgtcatac cttctgaatc
     6601 tttgacactt ccattttgat ttttattata aacctgtccc ttaaaacgat cggtattaaa
     6661 aacacagttc tgaattgtgt cacccttgtc cagactccct acaattccac ctgctatact
     6721 tcctgtcatg actgcatcgc tccagcagga gatgatatca caggcttctt gattcttata
     6781 gccttgataa gcaactatac cgccggcata atatccatct gctgttccgg tattataaca
     6841 atacttgata taggttctta catattgttt tgagctgttc atatcacaga cctgttcccc
     6901 ggcgatacct ccgctgcgtc cccagcccca cgttgccttt accgatccgc tgtttttgca
     6961 tgattcgatc cagccctgcg aacttccgac aatgccgccg ccgaaatcat gagctgttac
     7021 ttttccgttg ttccagcagt tgatcacttt tccaagattg ctttttgctg caactgctcc
     7081 ggccgtactc gttgacgatg tgaacacgcc atcttccatg ccaagatttt tgacaacgct
     7141 gttctccttc agctgtgcaa acaagcctga tacctgttta tcccctgtcc gctcgcagac
     7201 cagcttgctg atcttatgtc cgtttccgtc aaagattcct gtataaccgt catttgtatc
     7261 tccgatcggt gtccagtctt cacctgtcat atcaatgtct gccgtcagca ctgcattgac
     7321 agcgttctct ccggcattga ccttcgccgc aaacgcctgc agatcttctt tgtctgcaat
     7381 cgcataatat ccggcataat ctccgtttaa atgaaaatta ctctggttct cttcagtaac
     7441 ctgtaccagt cctgctgcat ttgcctgaat acttaccaag ccaccaaaat ccattgcggt
     7501 aaatgcaagt gcaaatgcaa gtaatcctgc tgcaattcgt tttctcatat cttcctcctg
     7561 ttttcctttt ttaatctttc attcctgact gattcctgta aattgctccg ttgaaaacta
     7621 attttcgcaa ttctatagat gccagggtca aaagcctgaa accacgatgt gcttgcacag
     7681 gagtggtttt atggcttaat gacccgcccc gatatgagca taaaagtgga gcgtaagctt
     7741 cacgatatgc gaatattggg tagaattgtg ggcgtacgaa gtacggaaca attcgtatgg
     7801 catcttttga ccccaacatc attctataac cattatataa aattaaaaaa gcattgtaca
     7861 ctagccaaac ggccagtttt acaatgctta atcgagtttt ttcaaagttt tctgttttta
     7921 catcgaaatc catcccagta catttgcaat ggagctgaga aggataatga tcacgcaggc
     7981 ggatgccaga tatttcagga agaaatgata taatttcttg cgtttaaaat cagatgactg
     8041 ttcaatctcc gcatccattc ttttaaatcc gacgactttc agaatcagaa tgcaggtagc
     8101 aagtgctgcg atcggcatca tgaccgagtt ggtcaggaag tcaaagaaat ccaaaaactg
     8161 cataccaagg atcttcacag agtcccagat accatatcca aaagaggatg ccgttccgat
     8221 caccagcatc acgacaacca tcagcagaca gcattttcca cggctccagt gaagctcatc
     8281 gttcagggtg gaaacgctgg tctcaagaag tgagattgca ctggtcagtg ccgcaaacag
     8341 aaacatcagg aagaatgcga tgccgacaat cgtgccaact cccatactcg caaatacttt
     8401 cggcagggtg atgaacgtca gtgccggtcc tgcctggagc gtttccatat ctccgccgga
     8461 gaatgcaaac actgccggaa tgatcatcag accggccaga agtgcgacac cggtgtcaaa
     8521 aatctcaacc tgcgtggtgc ttttttcgat gtctacatct ttcccgatat aagatccata
     8581 cgtatacagg atacccatcg cgatcgacag ggaataaaac atctgcccca tcgccgcaac
     8641 gaccgtcatc caggaaaaat tctccagatt cgggatcagg aaatatttga tgccttccac
     8701 ggctcccggt cttgtgatgg aataaacagc cacacaggct gccagcagga tcagaagcgg
     8761 catcataacc tttgaaacac gttctactcc attttttaca cctgcaagaa tgacaataaa
     8821 taccagtgcc gcaaagatca gaaaccatat ttctacttcc atggaagagg tgataaaact
     8881 gttgaaatag ccatcctgtg caacaaggtt tgcattgccc ctgatatact caaacagata
     8941 tttcagaacc cagccgccga tcacactgta ataaggaacg atcatcatcg ggatgactgc
     9001 gttgatccag cctcccaggt gaaacggcag gctctttccg aatttgtgga aagctccaac
     9061 cgggcttttt ttcgtcatac gtcccagtgc tgtttcggat acgatcataa catatccaaa
     9121 agtgaccatt aaaatcagat agacaagcag aaacatacct ccgccgtatt ttgccgcaag
     9181 atatggaaat cgccagatgt ttccaagtcc aaccgcagag ccggcaactg ccagcacata
     9241 accgatgcga ccggaaaatt tgccccgtgc gtgcaagaga ttttctgcat gttttttatt
     9301 tgtgcacata aaaagaatcc ccctgtggta aaaaatccct tacagcagaa actgcccgcc
     9361 gcaagggatt ttattattta aaacttacaa aatgattgtt ctcaagaatc tgaaaatact
     9421 ttacgattcg tggataaagg ggttctgccg cttcaccaaa ccgctctttt tccagagcag
     9481 ccagatccat gacggttctc tctccatcga taaaaggcca aagataactt ccatgtacat
     9541 ccagatgtat ctttgtgact tccgggcggt taaatacttt ctgtgcgatc ccgttaaaaa
     9601 aaccttcatt ttttacttcc agtatcacgc gatccctgaa gtctttgtgc cacttcaggc
     9661 cttcggcttt taccggaatc agatccagat aatttatctg ttgttcctgt ttcttttttt
     9721 tcatgctttt ttctttttct taagagagaa gtaaaacagg gatgcgatca tcagtaccat
     9781 aagaatgaca ccaccgatgt tgccaaagtt tacactctct ccgatgcttg tgttgatacc
     9841 aaatactgcg aaaattgcca gcaggatacc taccagacct tctccggcga tcatacctgc
     9901 acagtacagt gttccgtctg tggactgctg ttcttttgtc ttttcatcga cattcttacg
     9961 tgcatccatt accatacgta caacgccacc gatcatgatg gttgcattca ggtagatcgg
    10021 cagatacaga ccgattgcga acggcattac cgggatacgc aggatctcaa gacccagtgc
    10081 cagaaatacg ccgacaaata ccaggttcca tggcaggttt ccgcccatga taccttctac
    10141 gatcatcttc atcagagttg cctgaggagc cggtacttct gctccgccgt aaccccatgc
    10201 tgcattcaac aggtagagaa caccgccgat agccagaccg gatgcaagaa caccgatcag
    10261 ctcaccaatc tgctgttttt tcggtgtagc accaagcaga taaccggtct tcagatcctg
    10321 tgaagtatca cctgcgattg ctgcgataat acagataaca gaaccgattg cgattgctgc
    10381 tgtcataccg tcgatacctg ttttgccgct tcctttgatc gccatagttg cgatcagcag
    10441 agtagcgatt gccataccgg ataccgggtt attggaactt cctaccagac ctaccatacg
    10501 ggaggatacc gttgcgaaga agaatccgaa gattacgatc agtgctgctc caagcagagt
    10561 taccgggata gccggtactg cccagatgat aacaaccatg gcaacgattc cgatcaggat
    10621 aaactgcatc ggcagatcct gtgcagtacg tgcagtactt gtattttttc cgccttttac
    10681 gctcttcata gagtcgctga aagtttttac gatcagaggc agagatttga tcagagagat
    10741 gataccgcct gttgcgatgg cacctgcacc gatgtatttt acgtaagtac tccagatagc
    10801 tgatgcaccg ccgcctgcat aaagctgagc gattgttgtg cctgcttctg ccggatacag
    10861 ccaggtatca ggtccgaaca gacagatcag cgggatgata accagccatc cgaatacaga
    10921 accaacaaac atgtaagatg cgatctttgg tcctacaata taaccaacac caagcagtgc
    10981 cgggtatact tccataccga tctcaccttt gaaagattta aaagctgctg atacgtctgc
    11041 cgggatcacc ttcagaccat caacgataaa tttaaatact gctgcaagtc ccataccgga
    11101 gaataccgta gaagcgtttg ctccgccttc ttcgccggcc agaagtacgt ctgcacatgc
    11161 agttccttcc gggtaaagca gtgtcaggtg ctctttcacg atcagggcat tacgaagcgg
    11221 taccatgaaa agtacaccta aaacaccacc gcaaagtgcg atcagtgtga tctcgacaag
    11281 gctcggcata gagcaaagac cctcttctgc ccagaggaac agtgccggca tcgtaaagat
    11341 cgctcctgct gcaagtgact caccggctga accgatggtc tgtaccatgt tactttcaag
    11401 gatggaattc ttacgcatga taatgcgaat aacacccata gatataactg ctgccggaat
    11461 agaagcagaa acagtcatac caactcggag tccaagatat gcattggctg ctccgaaaat
    11521 tactgcgagc aaaacaccca taataatgga agtcacagta agctccggca tgactctgtc
    11581 tgccggaatg tacggtttga attcgtcttt cttattcatt tccttttcct ttcatacttt
    11641 tttcttttaa caatccgaaa accttcgtat atccgccctc ttccatctcc ggatcattat
    11701 ttacctaaaa taatccttat gaattgctcc gcttttccac agcttctgca attccgtatc
    11761 atcatagaac tatattgtac ctaaaatccg actaaattgc aagaatataa tgcatttttc
    11821 tacttttttc ctgtttttcc cactattttt ttgtgcactt tttgagatgc cagggtcaaa
    11881 agatcaaaat gtcgttcttg cttgcaagat aagacatttg aacttgggac ccgctaaa
//
LOCUS       DAWDWH010000039        11916 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_2874_length_11916_cov_17.646657, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000039 DAWDWH010000000
VERSION     DAWDWH010000039.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 11916)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11916)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11916
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2874_length_11916_cov_17.646657"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2717 a         3221 c         2362 g         3616 t
ORIGIN      
        1 ctgcaaaaac aactgtggaa gataatactg taaatgcaaa taccgtcgca ctgccgcaaa
       61 gtaccgtttt cattcttgct ttcatgtaaa aaaacctcct gaactcctat aacttcacgc
      121 ttttttggca tttttgttat atttgttaca aatgtattaa cttttgttac gtttgcatca
      181 tataacagtt acagtatttt gtcaaccttt ttttatcttc gtcaagtgat gagaagcaaa
      241 ggattgcact cccactaaga tagcctccgg cggattgcag aggcattctt gaattttaaa
      301 gtttgaggat ataccgcaaa aaaacattgc tctcataaag aaaggtgaga atcggacttt
      361 cttttatcat cgaaagcagc tggcttccga tctctgtgtt ggagcagatc gccactacca
      421 gaaagaacag ccagacgatg cagatccctt ttacaaatcc gagtgctcct ccgcacatcc
      481 ggttgacgcc acgcaggatc ggcagccttg tcatgatccg cagtgcccgt gcaacggcgt
      541 gcagggcgat acttaccagg ttaaaagaaa taaaataggt cagcatgttc aggatcatat
      601 ccgccagata ggtggaaata tattccgaaa aggtctgaac cgccatattt gcatacgtgt
      661 aactgttgtt attatcaaga agctgcgtct gcaattcttt cgggatcggc agtgatttga
      721 tgatcttgtt ctgatctttt cgcggcacat cgctctgctg tgcaaatttt tgtgtttctt
      781 tatccaattt gtccatgatc gcatcttcca ccagcgtctc gcatttgctc tccacaaatg
      841 agtagacggg tgtctgcgtt tccagacaat cattgacatt gggtgtcgaa taatagacca
      901 gtacacggga aaacccgaaa aaaagcagag aggtcagcgt cctcgcaaaa cctctcatat
      961 agccattgat cgcaaatacc agcagtacca ataagataat gatcgtaagt aaattcataa
     1021 tttcctttct gctatccaag ccggatcaga tctgcactgt cctcggtgat caccatatat
     1081 ttgcggaatc cacggatact cagcacattc tggattggct tgcggtattt gccctggaat
     1141 gttttctgac cgtttaactt ataaatggca aattcttttt cattgaacag cacgattttg
     1201 tctttctgca gggtaatact gttgtactgc agatcaaaat atttctgcat ggtattctta
     1261 ccgccggtat tgtagacctt catcagatat ttatgtccag agtcgcctcc gtcaaagaca
     1321 aagccgatgc ggttgccgtc atggaaagta ctgatgatct cctgatcaaa cttcaccgtt
     1381 ttcttttctt tcggagactg gctgccggaa aataccgtaa acccgtcggt gcggacggca
     1441 actgccgtgc tgttgtcaca gaaaaaaatc tgcggagcca gcacgtcgtc cacatttagc
     1501 tcaccgacct gattgttcag ctccatctga cctgcacttc cgaagttata aaacgcaata
     1561 tgtgtactga tcttgtttcc attgacccgc atataggaca ctgccatctt gatcccgtcc
     1621 ggagaaagtg ccatatccag cggataacca aaatcgtcga tcgtcgtgcg attctcggcg
     1681 atcagtgtcc cggatgcatc gtaaaaattg acccaggtca catcatcatc tgctaggatc
     1741 accgccacaa caccctgcgt tgccactctt gccttctgga tcggcagttt tgtctggaac
     1801 tgtcctttca gtccctcttc atcaaataca tagacctgca tcccctgctg atctgcgatc
     1861 acagcagtcg taccgcagac atccacgaac ggtgcctgca tactgtaggt cgtactccat
     1921 tttgtattcg atttgctgtc cacacaggct accccgtcgg agctgtattt caggaaataa
     1981 tctccaaact ccagatactg cgtactgctc acgtcatttc tctcaatgcg gtctgaggta
     2041 agataggtgc ttccggtgtg gaagaataaa aatgtcccaa gcccgaacag caggataaac
     2101 agaagtccca tggtgatcgc attgcggtta tgtacaaact tctgatgttt tttaatctta
     2161 tcccacagtt cttcttttgc ctgtggatct tccgtcatct catatgtctc gatgcggata
     2221 tccggatcgt tgagctcttc gttcagatcc aaatccagat catcctcttt ttttctgaat
     2281 aattctttta attttatttt catcctcatt tcgtccttac tgtgccaatt ctgaaatttt
     2341 cctgatgctc tgcaaaagtt cttcttaagt accctgcaat gttgttatta tagtttaaaa
     2401 ggcatgggat agtcaaccat gccttttacg ttccttattc tttttacggc agcacgatct
     2461 tctgtcccgg aagaatcgtg tttggatcgg taatgccgtt cagatcacag atttcctgaa
     2521 gcttgtccag atttccgtag tacatccggc agacgatcgc cagcgtatcg ccgtcccgca
     2581 ccgtgtattc tgcctgatac ttcgtcacat ccgaagagga tgccgcacgg ctatttgcag
     2641 aggaactgcc ggatgtggtg ctatcctgcc taccggaagt tccggcacct cctgttccat
     2701 cggttgctgt gctgctttcc gctgttcctg tgctgcctgt tgttcccata ttgtctgtgg
     2761 aatcgcctgt tgtcgtcgta ttgctctctg tactgctctc ttttaagatc caggtctttt
     2821 cagtctcatc ctttgcggca ctctcctttg caatttctcc ttctgcctgt gccgaagctg
     2881 ccaaagcatc cgtttctttc tgtttttcgg ttccttttga caggccactg ctctgcccat
     2941 cacttccaag tgccatctcc tgtagcgtca cttccatatc tttcatgcga ttatagttgt
     3001 tgaccatcgt cgccccgatc accaggatcg tcagcaccag aaacatactt gcaccatata
     3061 aaaaacgcac catcgtcgga gttttgcttt tctgctcctg ctccacgttc tgtgcgtcct
     3121 gttgtttttt tgcgatccgt ctgcggaagt tctggatggc tccatcctga ttctttgatt
     3181 ccgcctccac ggactgccct tcattttctt ttaccatata gctctgcatc tgttcgtttt
     3241 tttcgtagta gatgtaatag ccgctctgct ttcgaatccc ggtgttctcc gtcaggaaaa
     3301 cgctggcatt ttcttcgctg tcatacagaa acagcagcct gtcctcaccc ggaaactgtt
     3361 cccggtgcag atcaaagagc ttctcatccg gttcttcttc cggctcctcc atctttttcg
     3421 cccagccgat cacttctctt cccggaaaat actgtttcat ttcccggtag attttctccc
     3481 acatatcatc gtttggttca ccctctgcga tctctacggc tccgtccgca aacagacact
     3541 tcatgttttc gttctgccag acttcaccga gcagcaccgc acagcctgtc ttttccagaa
     3601 atgtgatcac ataatcttct atataaaccc ttttatctga tcctgcatct ccgatctgcc
     3661 ggatattttt cggcaggcct gtccgttcct ttccctttcc gtcctttgag gttcccctgt
     3721 aaaccatttc gatcatactc ccaatcctcc tttatactac ttccagctga tgcccctatc
     3781 gtagcacaaa gaatctgcaa attttagcaa tccgtgggat acttttcaag aactttccga
     3841 catgtttttc atcggattga tgcgaataaa ccttcctttt ttcggataca ctgtaaaagc
     3901 ctttaacacg gcaaattttt aagagaggga tgacaatcat gttttcttcc gatacgatct
     3961 gctattttaa tgcaggctct tccgataccg cagagcttct cggaaattat ctttctaaaa
     4021 ttctgattaa aaacggtttt tcagatatcg cacctgtcct tctatgcatc ggaagtgacc
     4081 gtgtgaccgg tgacagctta ggtccgatgg tcggcagtgc cctggaagaa cggtacaaaa
     4141 aaagcatccc cgtgtttggt acactgaaga tgcctgttca tgccctcaat cttgaggaaa
     4201 ccatcgatgc gatccatcta cattttccgg atcatccgct gatcgctgtc gatgcttcct
     4261 ttggaacaaa agaacatctt ggctgcatta cggccggcaa aggaagtctc tgtcccggtg
     4321 ccggcgtcga caaaaatctg attgcagtcg gtgacttttt tgtaaccgga atcgtagcat
     4381 ccttttctcc gttttcacat ctggtactgc agtcaacacg gctgtctgcc gtgatgccgc
     4441 tcgcctcgca gatttcccgc gggatcgctc atgccattga cgaaattgct cccggctata
     4501 gcctttcttc gcagatactt tgaaactgca tgactgatct tacatgcatt ttgtcctcat
     4561 tacagctcca cgcggccttc cagggcacgc agcagagtaa cttcatcaat atattccaga
     4621 tctccgccca ccggtacccc gctggcaatc cggctgactt tgataccggt tggcttgatc
     4681 agcttgctga tgtacatcgc cgttgtctca ccttccaggc tggaattggt agcaatgatc
     4741 acttcattga catcgccctg aagtcttgtc atcagctctt ttaattttat gtcccccggt
     4801 ccgatgccga gcatcggtga gattgccccg tgcagaacat ggtagacacc gtcatacttg
     4861 ccggtcttct cgtacgccgc cagatctctt gtattttcca cgaccatgat cgtcttgtgg
     4921 tctcttgcat cgttcttgca gatcgggcag agctcatcgt ctgtcagcgt aaaacattct
     4981 ttacagtatc tgacgttctt ccttgcatcg atgatcgtat ccgagagccg ctgcacttcc
     5041 tcgatcggac ggttaataat atgaaacgcc aggcgttggg ctgatttcgc ccccacaccc
     5101 ggcagtttgg acagttcttc gatcagtttt gtgatatgac tgctataata atccatcaga
     5161 acggaaatcc tccacccata ccagtaagtc cgcctgtcag ttttgacatg actgcgttgg
     5221 tctcttcctc tacttttctc agagcttcgt tggtagctgc catgatcaga tcttcaagca
     5281 tctcgatatc atccggatct acgacttctt ctttgagcga aactttggtc acttctttct
     5341 taccggagat cgtcacttct accacgccgc cgcctgctgc tgccgtaaac tctttctctt
     5401 ccagtgcttt ctggttttcc tccatctgac gctgcatttt ctgtgcctgt ttcatcagat
     5461 tgttcatatt gcccggaata cctccgcccg gaaatcctcc acgttttgcc attttctaat
     5521 cctcctgaat aatctttcta ttagtcatct tcgcaactgc tcaatacttc gcagttacga
     5581 tgcaaaaatc cattaaaaat tgctttgcaa taagattttt gcacacctga atcatattct
     5641 tacggtcttc gcagcaagtg ctccgacctg tgccgaatca ttagtcatct tcgatttcta
     5701 tatccgcatg gaccagattt tcaagtgcct cctcgacctg cactttggaa agtctggtat
     5761 ttaccacatg ctcatctgcc tgcaggatca tcttgacttt gacctgcctg cctgttttcg
     5821 ctgcaatgat ctgctcgagt acttctgctt tcttcggatc attgacataa gtctctccca
     5881 gaaaatcaga aaatacgata taaagcgtac catcttcttt ttctgtatta aatttcggaa
     5941 cggcacttgc cagaaccaca cggaaacgcc ctgaagtctg tgccaggatc gtccgccaca
     6001 taccgacgat cttctgcaga tcttccggtg ctgcctgctg aagctttgcc gggtctgtat
     6061 tgcccgctgt tccatccgga tctatgcctc ccggagccgc cacatttacc ggattttgat
     6121 agtcaaaatt gccctccggt atgtaaccac cggtgtcctg ctgtcttacc accacgcccg
     6181 atgccagctg cttttccagt ctccgcacgc gttccagtac ggagagctga tcctgctcca
     6241 tctgcggacg gcacagtttg atcagtgcca cctccagcat cactcgcttg gatcctgcat
     6301 atttgatttg attctgcaat tctgaaaaaa tgcggatata acgcatcagc gtatcatccc
     6361 gcaccatcgc tgcctcttct ttcagctgct ccagattctc tgcggagaca tccagcacat
     6421 cttccatatt atcggaactt ttaagtagaa gcaggtttcg cagataccag gtaaaatcgt
     6481 ttacaagctg cgtcagatca cgtccctgca tgatcatatt ttccagatgc aggatcacct
     6541 gcgtaacatc cccgtccagc acttcccgca gcagtttgct gaactcatcg gtatccaccg
     6601 caccgagtac atccagaaca tgctcatacg tcagcttttc tcctaaataa aaggcaatac
     6661 actgatccag aagacttaac gcatctcgca tggaaccgtc gccttttttg gcgatataac
     6721 gcagagcctt atcttcgact tcgacctgct cctcatccat cagctctcgc agacggtcca
     6781 cgatcgtcgt gcgggcgatc cgtttgaaat cataacgctg acatctggag agaatcgtga
     6841 tcgggatctt gtgtgcctcc gtcgtcgcca gaataaaaat gacataggaa ggcggttctt
     6901 ccagtgtctt aagcagtgca ttaaacgcac ctatagacag catatggacc tcgtcgatga
     6961 tgtagacctt gtacttgccg ctggtcggtg agtaggcaac ctcttcccgg atctcacgga
     7021 tgttgtccac accgttgttg gaagctgcgt cgatctcaat cacattcatg gaatttccag
     7081 cggcgatcgc cttgcaggtc tcacactcgt tgcagggatt tccatcaatg ggatgctcac
     7141 agtttaccgc ttttgcaagg atctttgcca ccgtcgtttt acctgttcct ctggtaccgc
     7201 agaacagata ggcatgtccg atacgctgtg ccctgatctg attttttaat gtccgcacaa
     7261 ttgcgtcctg gcccttcaca tcttggaagg agtcaggacg gaatttacgg tataaggcag
     7321 tatagctcat gctttccctc tgtttctaaa tacttcaaaa atgtctgtgg cagactctct
     7381 gcccttacat cagatgtgaa gtgactccca tccgataccg tctcggtttt atacaccaaa
     7441 gctgatgccg atgcattttg cttctttgat cagctgatca tccggagata ccatcttcag
     7501 cttgccggct acatcaccaa gcggtacttt cttgactttg ccgttgatga tacctaccat
     7561 gtaaccatat tcctttttca tgatcagagt tgctgcttct gcacccagtc tggttgacag
     7621 tacacggtca tacggacaag ggcttcctcc tcgctgtgta tgtcccggaa cggtaatacg
     7681 cacttcgata ccggtcttct ggaaaatctg ctctgccagt tcgtaggata cggatggatg
     7741 ttttctcttt ttgatctttt cttttaattc ttttttcgga agagctgcat cttctttgga
     7801 gattgcaccc tctgcaactg ccagaatggt aaaaccatgt ccggatgccg ctctcttgtg
     7861 gatcgcttcg atgatcttat cgatatcgta agggatctcc ggaagcagaa tgatatccgc
     7921 accgccggcg attcctgcat gaagcgtcag ccagccgact ttgtgtccca taacttcaac
     7981 taagaataca cggttgtggg atgctgcggt tgtgtggata cggtcgattg catcggtcgc
     8041 aatgtcaacc gcactctgga aaccgaatgt catatccgtt ccataaatat cattgtcgat
     8101 ggtctttggc agatggatga tgttcagtcc ctcttcacgc agcagatttg ctgttttctg
     8161 tgttccgttt cccccgagga tcaccaggca gtccagacgc agtttgtagt aattctgctt
     8221 catggcctct accttgtcca gtccgtgttc atccggtgta cgcatcagct tgaacggctg
     8281 tctggatgtt ccaaggatcg ttccgccctt tgtcaggatt ccagaaaaat cttttgcaga
     8341 aagcatacgg taattgccgt agatcagacc tttgtatcca tcataaaatc cataaacttc
     8401 gagttcctga acatttgcac tgagaccttt gactactcct cgcattgttg cattgagtgc
     8461 ctgacagtca ccgccgcttg tcagcatacc aattcttaac atttgctacc acgctcctct
     8521 ttttttaata ccttcttatg cagatgtcga agcaaaaaaa ctttcggcct tttcacttca
     8581 acggatgcct gcgaattgct tcgttacaaa gtaacgctcg caattctgta aacatttaat
     8641 ataaggcagg tggttcctgc cttcgacacc acctgccttg attatataat atttttcttc
     8701 ccactacaac ggtaaaatca ataactttct gtttccttat ccaggatcac ttccgcaaat
     8761 gttgctttcc tctccatcag ttttgtcagt tttgccattt ttgatacatt tccgatcaag
     8821 atcacaccgc agagctttcc gttcaggaaa tacagtttct catactgctt cttgctcctg
     8881 tcacagatct ccagtgtttt atagagaaga tttttgtttc tgccgttatc tccaatcgca
     8941 tacagggaag tattcatacc gttaaaggag agcggcatct gtactttctg atattccacg
     9001 cactccccgg ctgcattggc tcctgcgact tttccctgct cacttgcctc ggcccagagt
     9061 gctgcatttt ccccttcaaa ttccacgcag tctccgcacg catagatatc tttttcactg
     9121 gtctgcattt tttcatttac agcaactgcc ctgtttacct tcagtcccat ctgttctgca
     9181 agttctgtat ttgcacgcac accgcaggag atcagcacca gattggctgc gatttcttcc
     9241 ccatctgcaa gacgaacggc cgttacacgc tcctgccctt cgatccgatc gatctttact
     9301 ccggtgcgaa ccttgatacc gacactctct gcaatgcttc caagcatcgc tgccgcacct
     9361 tcatccagtt gtcttcccat cagaaccgga gctacctcga gaactgtcac ctgacatcct
     9421 gcctttttca gctcccaggc tgcctcaagt ccaagcacac caccgccgat cacgactgtc
     9481 tgtccgttct ttgggatcat ctgcccgatt ttttctgcat cttccagagt acggatcact
     9541 gccacctgct ctttttcgct tcccgggatc ggagggacga agctttttgc tcccagtgca
     9601 tagatcagtt tcgtgtattt cagacgcatg ccatcctcaa gtgtcacttc tttttccgtt
     9661 ttgtccactg aaataatctt tttgccgagg atctgataaa cctgattttc ctcataccag
     9721 ctctccggtt ccacctgaat ctgactgatc tcaagtccgg catttaaggc ttttgtcagc
     9781 atcggacggt tataggtcgg atatttttcc tccgagatca taagaatcga agatgtctta
     9841 tcccgctccc ggactgcttt cgccgcattc agtcctgctg taccgtttcc aaggatcaca
     9901 taaaattcat cgccgtcttt tcggtaagta gtttcttttt cttccaccgg cacaaaattt
     9961 tcacgtccga caccgcagac cggacatact tccagggaag aatcaaaaat ttctccgcac
    10021 accagacatt tcacaagctt tcgactgcct tttgtcgttt tctccgcctt tttatcctgc
    10081 aaaagagaac cgaaatgata gccgtattcg aaagcctcta caagctccgt atctcccgga
    10141 cggaaacgga tgcgaagtcc gtccactgta ttcatgcgaa gctgtttcag acgctccatg
    10201 atatgcggca cgccttctcc gctccagccg tagctgccga atgcacttgc cagtttcccg
    10261 ccatgtgttc ccgcaaaaat ggatgttgtc agatcccaga tcggctttaa tgcttcccca
    10321 acgatcgtcg gtgttccaaa taaaatacca tcagcaaatc caatctcttc cagtacctgt
    10381 gcctgatctg ctgtcaccat atcgtagctt cgcacatcaa tatcaccgct tgccttgatg
    10441 ccttcttcaa tcttttccgc gatctgcttt gtataaccat aggcacttac ataaggaata
    10501 atgactgttt ttttctgatt tggattgacc acggtacacc atctctcata agtatcctca
    10561 ataaagtcga ttccggaatc cagcaccggt ccatgtcccg ggcagatcat cgaaacatca
    10621 agctctctta cacgttccag agccttcagc ataaatggct tgaacggtcc aatgatgcag
    10681 tcaaaataat attttgttgc ccgcatatag ccttcccggt ctgtcacctt gctgaccagc
    10741 acatcctgaa atccatagtg ggagccgaag gaatcgcagg tcacaagcac ctgttcttct
    10801 tcaataaacg tgtacatcgt atccggccag tgcagattcg gaaccagcag aaaacgcagt
    10861 gttttgtccc cgatcttcat cgtctcgttg tcatgcacga tcagattgca gaattcgctg
    10921 tttacgatct cctttaaaaa tcccgctgca caggcagtgg aaatgatctt cagctgcgga
    10981 cttaatttca acagacgctg cacactgccc gcatgatccg gctccgtatg attgaccacc
    11041 agataatcga tctttgtcac atcgatcact tcgccgagtt tgtccatcca ctcctcaaaa
    11101 aaagcttctt ttaccgtttc aaataatacg gtctttccgc cgcttttgag cacataagaa
    11161 ttgtaggttg tgccaaattc cgtgtacatg ataatatcaa aaaccctgag cgtgtcatcc
    11221 acgattcccg cccagtaaaa atcatctcta agctttaatg ttttcatagc atacctcctg
    11281 tggttttgtg aatgctctat atcttcacag ctgcattttt tattcttgtt ttaaaatgcc
    11341 ctgtgcagct ttaagctttt tattatgttt agatgtgagt gtcaaaagtc tccaactatg
    11401 atgcctacgg attgttccgt gcgttgcact cccacaatcc tttctacgct ccgcttcggt
    11461 cggtgctaaa catgtgccac tggcacatag caccccagta ttcgcatatc gtgggattat
    11521 catcccactt ttatgctcat atcggggcgg gtcccaaggt ctttcatcca cctatgagca
    11581 agctcatgtg gatttagacc ttttgaccct ggcatcatgt ttaaattatc atttgctttt
    11641 ttgttatgtt tatactatac tttgtatcag cagaattttc tgttgcatat gcaacttttt
    11701 ccatcagaac gcagcaagtg ttcgaaaact cgcaagcaac cacggtacgg agtctatatc
    11761 ttctttctgt gcaaacgtac aatatttgaa gtgtataaaa gatgctggtc gacaaaaagg
    11821 aagaagctca gtgacgatat tgagcaatgt gcaaaatgaa taattcccaa gaaggcactt
    11881 ttacgtgccc ttcggggaat tcgttcattt gcacat
//
LOCUS       DAWDWH010000040        11909 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_2876_length_11909_cov_13.802430, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000040 DAWDWH010000000
VERSION     DAWDWH010000040.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 11909)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11909)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11909
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2876_length_11909_cov_13.802430"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5121..5130
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8671..8680
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10058..10067
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2601 a         3025 c         2350 g         3903 t
ORIGIN      
        1 atacgacatc gtccatcttg cttccggtat cgacaagtgc cgttgccagg atcgtcaggc
       61 ttccgccctc acgcatattt ctcgctgctc cgaagaaacg tttcggcata tgcagtgccg
      121 ccgggtcaag accgccggat aaggtacgtc cgcttggcgg aaccgtcagg ttgtaggctc
      181 ttgcaagtct tgtgatgctg tcgagcagga tcgtaacgtc ttttttatgt tcaaccagac
      241 gctttgcacg ctcgatcacc atctcggaaa ctcgtttgtg atgctccggc agctcatcaa
      301 aggtagaata gattacttct acattatctc cgacaatggc ttccttgatg tcggtaactt
      361 cctccggacg ctcgtcgatc agcagaatga tcaagtgcat atccgggtca tttccaagga
      421 tggactttgc catctctttt aacagtgtcg tctttccggc tttcggcggt gacacgatca
      481 ttcctcgctg tcccttgccg accggtgaga tcaggtcaac gatacgcatg gctacatttc
      541 tcgaatcacg ctccatacgc atacgctggt tcggaaagat cggtgtcata tcctcgaaat
      601 tgcatctttt ggcagcttct gccgggtgca tgccgtttgt gctttttaaa tagagcaggg
      661 ctgcaaactt ctcctgctgc gtggtatttg tgcggatacg tgtattgccg ctgatgatat
      721 ctccggtctt cagattgaat cttcggatct gggacggcga aacataaata tcatggtcac
      781 ccggcagata gttatcggat cggataaatc cgaaaccatc cggcattact tccagaatac
      841 cggtcactgt ctgaccgctg tcaagctgtg aaaaatccag tcctgttttc tcttccggac
      901 gcttttcgga tctctctgca tgctctgtca tctctggtct ttccgctttt acgttctgtg
      961 tctgtgcacg ttcttccgtc tcttcggata ttttctgctc acgttctttt tcatctgcct
     1021 caagcattct tgtaactact tcactctttt tcagaccgga aagatttttc aggcctctgg
     1081 cttttgcaat cgcctttaat tcaaccgctg agagcgattc atatttttct ttcatcatta
     1141 tcctgtattt cctccatctt tgatggctgt ttctgaatct aacatctgcc tattttaaca
     1201 taactgtttt taaaaatcta tatgaacttt tacacgaaat gcttttaaaa agcaagcatt
     1261 ccgcctaaca ttccgcctcc catgcacaga caggctgtcg ttatcgtctg tatggaagac
     1321 ggatctatgt tccgtttcag gatcagtgtc agaagaaaaa gcactgcaaa ataacacatt
     1381 cctgcgagca gcccccacag gtattttttg ttctccgtcc ttctgcctgc gatccttccg
     1441 cccaaaaagg cggatcccag ataaatgacg gtcacggccg ccgagatctt tccttcttct
     1501 aactgaaatt tcatcatcgc agctgccagc agcacaagaa gcactgctgt gacgatccag
     1561 gaacccagaa gtgaaatgag cagggtcact ggttttgatc gtttctgcat tttttccatg
     1621 caatcccccc tgctcatact atatgacagc cttccatccg gcagaacatt ttttcaagaa
     1681 tgcctctggc attcttgctg cgaggtgcac gtcatgcatc gcatgacata ttcttctgtg
     1741 cacctctgca atccgccgga tgctatatca gatgggagat tgaatcccac cactgataca
     1801 agcttgttgc tcgccgcctg cagaggcggg aatcatctca catctgattt atctttcatc
     1861 tcatcaccgg ccatacgcag aacatctttg atctcctcaa gcggcatttc cagataggcc
     1921 gacagttcac tcatggaaac tttgtgtcca agatcctctt ccaggctgag gatcgactcg
     1981 ttcaggtgat tgactctgcc tacgatctcg tcatccaggt cttttttgtc ctgtgacatc
     2041 tcaacggcct cttccattgc tttccggata ccgtcaaaca cctgcttacg gtattcttcc
     2101 agatttggct ttgcctccag attttcaagt gcaagcagca gtccgatatt gccttcctgc
     2161 accagatctt cgatcggcag ctcggaagcg acatacgtca tggcaagctc gtagaccgtt
     2221 tccagatact gtcctgccag cgtgcttttt gccatggaat cgccatttac cgcctcgaaa
     2281 aacagacgtt cttcttccgt ctcggaaagt ttttcgatct ctgcaagttc ctccagatac
     2341 ataccaagga aatccggacc ttccccgtcc tgtgtttcac tgttttcttc ctgtttttct
     2401 gtctcaggat tcgaagcaga cgctctctct gcatgctcct tcactgtctt ctgtgcggtt
     2461 tccggtgtct ttttggccgg ttcgtacccg tccactttga tcttctggct gacaaggtat
     2521 tcgcagacca gtccaaactg ttcctcattc agggcatctc ccggaaagaa tttttttatg
     2581 tcttcctttg taagataatt tccctgtaca tctgcaagtg ccttcagctc acttaacatc
     2641 tctctgaaaa caattttact ctgcatcgtt tttttcctcc tgaagtattg aaatggcttt
     2701 ctgcaactga ttatcctctt cttccgtgac cgtcacttta tttttcagac tttcatcaag
     2761 ctcaacggtc acatccggct caatgccttt tttgtggata tccgtgccgt ttggcgtgaa
     2821 atacttggaa accgtcagtt tgactgccgt tccgtcctcc attccgacaa tccgctgcac
     2881 aatccctttt ccatacgttt tcgtaccgac taaagtacca atcccatagt ccttgattgc
     2941 tccgctgaag atttctgccg cactggcact gtacccgttg accagcactg ccagcggctt
     3001 tttgaactca ttgtttccgt cgcaggtata ttctgttctc tgcccgtatt tatcttcggt
     3061 ataaacgatc agtcctttcg gaagcatctg ttttaacatc tcgcagcagg tatccagcac
     3121 accgccaagg ttgtttcgca ggtctatgat cagcttttcc ataccctgtt cttctaattt
     3181 cgccagagca ttcttgaact gctccgccgt caccttgtca aactcactga ctgcgatata
     3241 accgatctca tcatccagca ttttgtaggc caccgtcggg acggcaacat ctgagcgttc
     3301 cacatcgaac tccagataat ccttttcacc ctctctggcg attgtcagat ggacggtgtc
     3361 tccttttgct gtcttgatca ggctgaccac atcggtcaga tctttcccgg tcacttcgat
     3421 gtcattgacc gaatagagaa tgtctcccgg aagcagccct gccttttctg ccggtgtccc
     3481 ctcaaaacag gtcacgacct ggattgcccc cgtctgtgta tcctgtgaaa gctttgcacc
     3541 gatccccttg tactctccgg atgtggattc attcagtttt tcgagttcat cctgtgtata
     3601 atatgcagca taaggatctc caagtccgct gatcatgccc ttataaatgc cggcttcgac
     3661 cgtatcgtta tccgtactgt caagatagag ctgattgatg atctcttcca tcttcttgat
     3721 ctttgtatct acctttttct gatccagact gagctgtgtg cctgcactgt ctgtgctctc
     3781 ctgcgttgcc cctgcttctt tctgatctgc tcctgtgatc cagccgccct gctgaagcac
     3841 cccgcttccg gccagaacga ccacagccga agtcaggatt ccacagaaaa aaccttttcc
     3901 gaacttcttg tcctttgcca tatctttgct ccttatatct tccccggtac agaaacgcat
     3961 cttttctgta acccaagaaa agaccggctt tgcacgcggc ttctccggtc ttcttctgtt
     4021 tgttgctatt ttatgtgtcc atgctgcggt ttatgacgga ggcggtatta ataatccagc
     4081 gtctccatgt acttcatata tttgactacc atcagtgcga tcttgaatgt gattgcatct
     4141 tcaaatacac gcagatccag tccggtactc ttctgcagct tatccaggcg gtataccagt
     4201 gtattcctgt ggatatacag ctgtctggat gtctctgaaa cgttcaggct gttttcaaag
     4261 aatttgttga tcgtaaccag cgtctcttca tcgaaatcat ccggtgattt gttctcgaaa
     4321 atctctttga taaacatctt gcagagcggg atcggaagct ggtaaatcag acggccgatg
     4381 ccaagagaac tgtatgcgat gatatttttc tcctcgaaaa agatccttcc gacatccatt
     4441 gccatacgtg cttctttata agatctggaa acttctttga tctcttctac gatcgttcca
     4501 tatgcaatat gcaatacttc ctgcttttct gccggcagaa catttaaaat ctgctttgcc
     4561 gttttctcca tttcctgata accttcttcg gaagccagct ctttgacaac gataatgctc
     4621 ttttcatcaa ctgctgtaat aaaatcacct gtcttggttc caagcagtga acggatacat
     4681 tccatggaag cattatcctt ctcctgagcc gtatcaacca gaaataccac tcttctcgca
     4741 tttgctgcaa tgtgaagctt cttcgcacgg ttataaatat ctaccagaag caggttgtcc
     4801 agaagctcat cgtatatttt gaaaaactgg tgtccctgta ccacctggct gtctgccggt
     4861 gaatctacaa acggaacgac tgaggaacga tattcttctg actccggaaa cgttgcagcc
     4921 agcacattgc cttccagatc gatcacgcaa aaatcaatcc tcgatatttc ttttaatcct
     4981 tctatggttg actgtaatat ctggtttgat atcatgtctt tctctccctt ccatacattt
     5041 tcataccaga ccaactacgc ttattaatct gacttgacta ttattgtaat ataaatcacc
     5101 aaaaaagaaa agagaaaatt nnnnnnnnnn cagccctttt tcgggtaaaa atccttcgat
     5161 tttatcgact ttttatttca cttttacctt ttttgcagtg gacattgcac ttgcatttcc
     5221 ttttttgacc gtcacgatct tgtagtagta attacctttc ttcctgatct tcttatcctg
     5281 ataagaaacg gtcttattgt tgttgatctt cttgatccgt ttataaccgg agtttttctt
     5341 cgtagaacga tagatcacat aaccggttgc ttttttattt ctcttccagc tcagttttac
     5401 actcttgcct gaagcagctg ctttcttgat ttttacattt gctgtaaact tcacagtctt
     5461 agctgcaccg ttgtcacttg cttttacgtt tgcatcctta gatactgcta ctgcacggta
     5521 agatgcacgc ttgatgcctt tcaaagaagc ggctttttta tctttaaata ccagcttgcc
     5581 ggatgcagtg cttcctgcca gaaccgtctt gccgtttacg gtgcggtaaa tatcataatg
     5641 atctgcacct tccactgcat tcacgatcac ttttacaacc acacctgctt ttgctgcact
     5701 tgctttcaca gacttgatcg tcggtgctgc cagtttcttc tctgccggtg tcggagtcgg
     5761 atttggttct gtatccggtg tctctgctgt tttcagagct gcctgtgcct tttcaagagc
     5821 tgctgtcaga tctttcagag cagctgccgt aagatcagca cctgcttctt ttgctgcctt
     5881 gtatgctttt tcaaatgcat cccaggattc tttggtgtac tgtccctgac ctgctgcata
     5941 aacagcatct gcctttgtaa ccgcatcact cagagctttc tgtgcttctt ctttgtcttt
     6001 gtcaaccgta tcttttttct gcagagcatc gattgcatcc tgtactgctt tctttgctgc
     6061 tgctacttct gtttcgcttg cattttcttt tgcaagtacg gcttctgcat ttgtgatcgc
     6121 tgtttccagt gcttttacag aatcctcggt atacagcgtt ttgtcgattg ctttcgcatt
     6181 gtttacggta ttgccaagtt ctgttctgga agcttccgtt gctttgtcag atgttacggt
     6241 aaccgttatc tctttttctg ctgtttttcc gttcagttct gcaactactt ttacttttgc
     6301 tgttcctgct gcacttactg tcagtacatc accttccaga gctgcctttg ttgtgccggc
     6361 atctaccagt gtataagtgt aagtgatgtt cttttcatgt gctgcacagt ctgctgcggt
     6421 tgcttttgca gccagtttca gtgttttgct tgtatctgct gctgccatct ggatggtctg
     6481 gttatcaaat acaagatctg tgatctcaca ggtacatggt acttcctctg tcttaaagct
     6541 gtaggaaacg gtcatggatc tgtaatcagc tgcaacattt acttccggtt tcacacttgt
     6601 accgtcagag agtttgatgc tctgcggttt gctgctgtct gcgaaacgat aatcgccaac
     6661 tgctgtcagg actgttttta cttcatagtt tgcatttgcc tcaaatgctg cacctgcttc
     6721 tacttctttg ccgtccttat accaggttgt gctggttgta tacggtgtca gtgcaatgtt
     6781 tacagacggt gttgcctgat ccagtgcatg agctgtcact gcataagatt tctgcagatt
     6841 atcagagtaa tcttttccgc tgtagatgcg gatgtcagaa agcagtccgg tatatggagg
     6901 tgttgttgag gaaccaagat ctttgctgat gttataacca gttgtaaagg tacttgtgtt
     6961 gtaagatttc agggttacat cgtaattgtc gcttgcgttt gctcttccgg atgtcggtgt
     7021 tccggctttg ccgtctacat agatctgcat ctttgtaccg gtaaatactg ccagaatatc
     7081 atgccattta ttccagaaac tggaatcgat cgcacagctg ctctcaaccc aggaaccttt
     7141 tgcgtcgcat gcataaacaa gtacgccgct gctcttgagc tgaacaccat actgctcatc
     7201 catcttgcca agcatttgat atgtaccgct tcctgtgatc tcatctgatt tcatacggaa
     7261 acggataaac attgctttgt cttctccgga aacatcaaat tttccgttgt ttgttccatc
     7321 attctctgcc gttacgtatc cggaatatcc ccatccatca ctgtaggata cctgaacgtt
     7381 atcattcagc gtcagtgttg ccggatctgc agcctggtct gcaactgcct caaaatgttt
     7441 gccttctttt gatgaagata cgactgcatc tgcggatgtc tcacctgctg ccggtgcagt
     7501 cacagtgatt tccggtgctt cgagcacatt ttcttctttc ggtgcctcca caaattttgc
     7561 aagaacattc agctctgctg cattgatgaa catattcggt tctgcacctt cactgtgaat
     7621 tgcttcaaat ttaacattgg ttacgcctgc aaccggctcg aactcaacgc tcttctcagt
     7681 cgggatggtt tctccttcat aagaccatgt tacatttgct acttttgtat aagtttctcc
     7741 gttatcagtg gatacataaa cgtttgcttc tgttacggca ccgtttttga taacaccatt
     7801 tttgtctctt tccggtcttg ggagataagt cagtgctttt actgcatatg ccttatccag
     7861 agtgatcgta taattgttgt tgccggtcag tgttccgttg gaatactgtg ccttcggttt
     7921 gctgttgtca ttccagtttg tatgccagta tgtgctgctg tcaccgtcaa gtgctgctgc
     7981 tatgataccg tttccggctc cttcgcctgt caactcttca ctgtctgcgg cacctgtcat
     8041 atttgccgga agcaattttt tataaccgtc atctgccggc tgattctctc tttttacaag
     8101 ctctgcaatg gcatcatcca gtgctttgac tgctgctgct ttttcttcaa cggttgcatt
     8161 ttcttttgct gctactgctt ctgctgcttt gatcgctgct ttcagaactg cataagattc
     8221 tgcggtatac tgatcttcat ccagttcttt tgctgcactg attgcatctg tcagtgcact
     8281 ttctgctgta gaatcagatt cttttgtata catttcatag attgccggag ctgctcctgt
     8341 gccatcccat gtgatcttca cttcgttgat accattgtag aataccagat tcttgaagac
     8401 attcagcgat ttggacagag taccgacttc tacccattta cctgcattgg tatggataga
     8461 aacttttgca ttggaaatct cctctccatc ctgaatgacg taaaggctgt cttttacacc
     8521 tgcatccaga cggtaagtca gagttcctgc accttcgcct gccctgaaca tggttgttaa
     8581 agaattatcg attgcctgtt ttgcatcaaa tccttccacg cctgccggtt ctgcaaagat
     8641 ggtcttgtct gcatcggtgt tggtcaggct nnnnnnnnnn catccatgcc tttttcggta
     8701 tcaaccggat accagttgaa tccattatag ctgtactcaa aggttacctt atctttgttt
     8761 gtgtaatctg ctgcaatcgt gctgatctca cgtacatttc cgaaatcaat accaatgtac
     8821 tcaccatttt tcaggataac atctttgact tccggaattg cagcctcatt tttattgatc
     8881 gttacaattg tctctttgta agtttctgtg ttggtgtatg catactcatc tgccgggaaa
     8941 gtctctgcct ggaattcacg gattgcatac catttgcttg ttgcagccgg tgtacgcaga
     9001 cggatgtagc gagctttcag ggactggttt gtcagatcca cgttgatctc gtttgttccg
     9061 taagtaccat caacctgtgt ccagttttca ttatccatgg aatactccag gataccctgc
     9121 gagaaaatat ctccatctgt ctgagttctt ccctgcagga tgcggacttt gccaagttta
     9181 taagctgctc cgagatccag cccaatgtat ccatttgcag taacacttgc attaaaccat
     9241 gcaaaggtac tgtcatttcc atcaataaca ttgcctgcat ttccctggta gaatccacca
     9301 agtccgctgt acatcagtct cttctcgccg gtctctgtgc caccgccgga agtatttccg
     9361 ccactgaaag tattatccag cactttcagt gctgctttct tcacataatt ttccatctgt
     9421 gctgcgaacg gcatgatctt catagctccg gcttctgcca tgatagtgcc gttcagctga
     9481 ggtgccgcac agttctgtga cagtttgtaa ttgttttctg cacttacata atttgcacat
     9541 acagcatcgt catcgcctgc tttgagtccc attgcagtct tgacaaatcc gatgcttgct
     9601 ttggaaagat atctcagaga atctacccaa ggtttcatct cttcttttag gttttcgttt
     9661 gttccgtttg tctggaaatc atctgcagct gtcacgatct tctgcagctg ttcaatcagt
     9721 gcatttccgg attccgtcag atctgcattg ctgttgtatg cagtcttgaa tgcattgatg
     9781 tacggagtca gttctaccga ctcacccatc gctgtgatac cgcccgggct cgggttagtc
     9841 atatgtttgc acagttcatg cagggactgt ggagctccgc tgtcgatgta cttgaagccg
     9901 tcagcccagc tcttctctgc atcaaagtca gctgtgttcc atgcaaaatc agcgatcgca
     9961 aacagtgctg tcttggaagc ttctgcctgc tccagcgggt tcgttacaat acccatgaag
    10021 tttgtcagac ctttttcaag gattgcactg tctccagnnn nnnnnnnatt taccggccag
    10081 ttcagccaca taaatgcctc tctgccgttt gtataagaat ttttaaaatt attgaaagta
    10141 ctctgtcttg ccggataaca tacagattca ccggtccaca tcagttctac atccggaaga
    10201 ctttcaaaat gtttgaagta gtttgcaacg gtctgagctt cgccttcact ccagctgctc
    10261 cagtttactg cacctgcaca gtatacctgc ggtacgaagc acagattgta aacatcgcct
    10321 ttggatttgc accatgcatc caggtctttg caaagtcttg catgtaggct tactttgccg
    10381 gctgcgtcgt ctgcggaaag cacaaactga cgtacgccaa cgttgtaaag ctgttcaaat
    10441 tttgcaatga taaccttcag gtctgcatca taagtggagg ctgtgatagc actgtgcata
    10501 aacggatgaa ttgcccatgc aaaacggcac ttggaatcct gtcctgcctg caccatctgt
    10561 ctgatctctt ccagtctgtc tgccggatag agctcacgcc actgtgaatt gtggtatggc
    10621 tcgtccttcg gtgcatagat atacatattc atcttgaaat cgccgccgaa cttcatcaga
    10681 ctgatacgat cttccacaga ccacggaata ccatagtagc cttcgataaa tccacggtac
    10741 tgaccggatg cgtaatcttc gatctgaagt ttgcgtacgg ctttttctgt cacctggtta
    10801 aaaatcattt tcagagagct cagtccgtaa aatgctgcat cggtgtcttt accaaggact
    10861 gcgatcacat tgtcctggat agccagaaca tatgcatctc tcttgtcaaa cagatcatct
    10921 gcataagtaa catggtcgtt gaaccatttt tcaactgctc catcggaacc ttttgtacca
    10981 accagtacgt tcattccgtt tgaagctacg gcacctgtca cggttcctgt gatattctga
    11041 atagccagta cttcgttcag acgtttctgt gttgcctcgt cgatcgtgct gtcatagaca
    11101 acgtttgtct tatctgccag ggtaaaactg ctatcctggt aaacaacact tctcggggtt
    11161 ggataaattt catattctgc tgtcgcagaa gtgtcatttg cagcatctgc tgcttttgca
    11221 tcaaatccgg taaaagtcat gcctgttaaa atcatggcaa aaaccagtac aagactgatg
    11281 acttttttca cattctttcc catctaaatc ctccttttca taagttcgac accgggaatc
    11341 cgtatgcagc ttccgtctcc cgcccgtcga tcgttttctt atttttcgaa gaaaatcttt
    11401 tgttgttaaa gtgcaaattt tctcacgaaa taagtacctt tattatacat tttatctgtt
    11461 ttactgtcaa tctcgaatca gtttcttatg caatttaagc aggaatattt gtgaaattct
    11521 tcgctttttt cgtcgaaaat ttcaaaagtc tgtactattc ttttcattca taacaaaaag
    11581 ccccgggaaa tggggctttc tgctgaaaaa tgtatgtaag aaattggaaa acggcgaaca
    11641 aaaaggcgct ccgtataaac ggaacgcctt tgttcttaca agaaacagta tacacatttt
    11701 ccagcatttg tcaaatactt tttcagatat tttttggcat ctcggaatgt gcaaaatgct
    11761 ctgcatttgt ctttcaatgc gttcatattt tattcatgat acattcaaaa aactttcatt
    11821 cgctcatcat gccaaattca tttttccctt ttatacttcc aaatgtatcg gagatgtcac
    11881 agaacatttt cagatatatg ctatatacg
//
LOCUS       DAWDWH010000041        11478 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_2991_length_11478_cov_11.144270, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000041 DAWDWH010000000
VERSION     DAWDWH010000041.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 11478)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11478)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11478
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2991_length_11478_cov_11.144270"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    446..455
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2227..2317
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6007..6016
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7856..7865
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8777..8786
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3427 a         2243 c         3203 g         2474 t
ORIGIN      
        1 cgttgccagc atggcaggca atatcacaaa cgaactggtc agcaaactgg gcaagaagag
       61 cctgtcaaaa gggatccgtg taaaggtaga agacggtatt gtaaatgtaa atgtggcact
      121 caacattgca tatggataca gcgtaccgaa gacctgcaaa aaggttcagg aaaaggtaaa
      181 agcagcgatc gagaatatga ccggtcttga agtagagaaa gttgatatcc agattgcaaa
      241 tgtaagcata tcaaaagaaa aataaaacag cttaaaagca gcgtaaaagt ccgttcccaa
      301 ggcgtactta aactccggat ggtgatcgga ttttttacgt tttcttttaa agacagcgga
      361 ggaaaaagtg actagacgag aggaacgaga acatattttt aaattactct ttctgacaga
      421 gttctataag gaagaggagc ttgagnnnnn nnnnngaagg gatttccgat gcaaaacagg
      481 aagaccttac ttatatcaga aaaaaatatg agaagatcaa agaaaatctg gaacagattg
      541 acgggcagat caatgaagcg gcaaagggct ggagaacaga ccgtatgggg aaagtagagc
      601 ttactctgct gcgtcttgca gtctatgaga tgaagtatga tgacgatatc ccacagggag
      661 ttgcgatcaa cgaagcagtt gagcttgcca aaattttcgg aggcgatacc tcttctgcat
      721 ttattaacgg cgttctggct aaactggtat aaagagccct tgactgccgg atgacaaaag
      781 aggtgggtgc gtgcagaatg tctatactgt gagacaggta aatgcctaca tcaagtccat
      841 gtttacacag gatgtggtgc tgcggagcat ttacatgaaa ggagaagtct caaactgtaa
      901 atatcataca tcgggacaca tctatttttc gctgaaagat gagagcggaa cgatttcctg
      961 tgtcatgttt gccagtgcca gaaagggact ggcttttggc atgaaaaacg gtcagcaggt
     1021 tcttgtgctt ggcaacatca gtgtttatga aagagacggc acatatcagc tctatgcatc
     1081 aaggattctg ttagacggtg ccggacttct ctatgagaag tttgaggcac tgaaagcaga
     1141 attagaagag atggggatgt ttgatcccgt ttataagaaa ccgattccgc cctttgtgaa
     1201 gaaactggga gttgtgacag caccgaccgg tgccgctgtg cgggatatca ttcagatttc
     1261 cagaagacgc aatccgggta ttcagatcat cctctatccg gcactggtgc agggcgaagg
     1321 tgccgcagaa agtgtggcaa aaggcattgc catgctggat gcgtatggcg ttgatgtgat
     1381 tatcgccggg cgtggcggtg gctcgatcga ggatctctgg gcatttaatg aagaagaaac
     1441 ggcaagagcg atcttttcct gcaatacccc ggtgatctca gcggtaggtc atgagacgga
     1501 cactacaatc gcagattatg tggcagattt gcgtgctccg acgccatcgg cggcagcaga
     1561 gcttgcagtt gcggatattt cagccctcca gggcgagtta taccgccaga agagccggct
     1621 ctttgaggct gtaaccagaa aagtgcagga taaaagggat gagctggcaa gaagagagaa
     1681 aatcctctcc cttttaggac cgcaggagaa gcttcaggat cagaaaatgc gtcttgcaag
     1741 ggcacaggaa gatctggcgg aggccatgca gcagaagctt gcgttgtcca gacaggagat
     1801 ggagtatgac aggcagcgtc tgcaggtgct gatgcgggaa aaatacaggg acagcaggca
     1861 ccggatgcag atttatatcg aaaagttaaa gggactgtct ccgcttgaaa agctggggca
     1921 gggctattct tatactgcgg acgtacacgg caggaagatc acacagaccg ggcaggtcaa
     1981 aaagggggac acgatctgtg tgtatgtatc ggacggacgg ctgcaggcga aggtaacgga
     2041 tattcagaaa gacaagcagg atgcaggtga ggtgtaaaga tggcaaaaca gacagaaagt
     2101 acagaaaaga aagaaaaaga acggacactg gaagaagcat ttctgcgtct ggatgagatc
     2161 atccagaaac tggagaagaa agatacaaca ctggaggatt cttttctggg atatcaggaa
     2221 ggcatannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttc ttgaggcgat gaattacagt
     2341 atgcgtgcgg gaggcaaacg cctccggccg cttctgatgc tggagactta ccggatgttt
     2401 ggcggagaag ggcagatcat cgagcctttt atggcagcga tggagatgat acatacccac
     2461 tcactgatcc acgatgatct cccggcactg gataacgacg agtaccgcag aggccgcagg
     2521 acaacgcaca ttgtatatgg ggaagcgatg gcaatcttgg caggcgatgc actgctcaat
     2581 tatgcctatg agacggcagt gcgtgccttt gagatcgaac cgggcaatcc ggcgatcggg
     2641 aaagcactgc agattctgac gaaaaagaca gggatttacg ggatgcttgg cggtcagtgc
     2701 gtggatgtgg aagctgcagg cagagagatt tccagagaga aactggattt tatttaccgt
     2761 ctgaaaacaa gtgctctgat cgaagcgtcc atgatggtcg gtgcggcact ggccggggca
     2821 tcggaggcag aaataaaagt gatcgaagat tccgcaagag atatcgggct ggcatttcag
     2881 atccgggatg acattttaga tgtcacaagc acgacggacg agcttggcaa gccggtattc
     2941 agcgatgaga aaaatgacaa gactacttac gtgacgatcg aagggatcga aaaagcaaag
     3001 agcgatgtgg aagagatttc aaaacgtgca gtttctgcct tacagtcgct tggccgaaaa
     3061 aatgattttc tggaagagct ggtgctgatg ctggtcaacc ggaaaaaata aggagcgatt
     3121 gactatggta ttagagaaga taaatcatcc gaatgatatc aaaaatctga aaaaggaaga
     3181 gctgccactg cttgcagatg agatccggga gttcctgatc cgcacgatca gcgagagcgg
     3241 aggacatctg gcatcgaacc ttggtgtggt ggaacttacc atggcgatgc atctggtgtt
     3301 tgatcttccg aaagataaga tgatatggga tgtcggacac caggcttaca cacataaact
     3361 gctgactggc cgcagagaga attttgcaaa tctgcgtaaa ttcgggggga tgagcggttt
     3421 cccgaaacgc tgtgagagca gctgtgacag ttttgataca ggacacagct ccacctccat
     3481 atcggcaggg cttggttatg tggcggcaag agatctgcag ggagaagatt attccgtgat
     3541 ctcggtgatc ggagatggat ctctgaccgg cggcatggca tacgaggcga tgaacaatgc
     3601 tgccaggctg aaaagcaatt ttatcattgt attaaatgac aacaatatgt cgatttcgga
     3661 gaacgtcggc ggaatgtcgg actatctcgg caaacttcgc acaaacgatg cctatcagga
     3721 attaaaacag ggcgtttcgg atacattaaa aaaaatcccg gtttacggcg accgcgtggt
     3781 ccgcaggatc aaaaagacaa aaagcgggat caaacagctg tttattccgg ggatgtggtt
     3841 tgaagagatg gggctgactt acttagggcc gatcgatgga cacgatatcg gcagactggt
     3901 gcgtgctttc aaggaggcac gcaaggtgaa cggaccggtg cttcttcata tactgacgca
     3961 gaaaggcaga ggatatgtac cggcggaacg tcatccggcg agattccatg gggcagagcc
     4021 gtttgacatt gccaccggac ttccgctcca taagcggaca aaagccaact atacggatgt
     4081 gtttgcgacc gttatgcgaa aaatggggga gaggcaggag aatcttgtgg caatcacggc
     4141 agcaatgccg gacggaaccg gactgaaacg gtttaaaaat gtctttccgg agcgtttctt
     4201 tgatgtgggg atcgcagaac agcatgcagt gacctttgca gccggtctgg cagcgggcgg
     4261 catgatcccg gtcgtggcgg tgtattcatc ctttttacag cgtgcttttg accagatcgt
     4321 gcacgatgtc tgtatccaga acctgcatgt gatctttgca gttgaccgtg caggtctggt
     4381 tggcagtgac ggagagacgc atcaggggat ttttgatctc tcctatttat ctctgatccc
     4441 gaatatgtgc gtgatggcac caaaaaacaa atgggagctg tccgatatgc tgaaatttgc
     4501 aatttcctct gacggtccga tcgccctgcg ttatccaagg ggcgaagcct atgacgggct
     4561 ttctgagcat cgtgctaaga tcgtatacgg caaaagcgaa ttgctctatg atgaagaatg
     4621 cattgccctg atcgcagtcg gcagcatggt aaagacagca catgaagtgc gtcagaattt
     4681 gaaacgcaaa ggttttgagt gtaccctgat caacgcacgg tttgtaaaac cgcttgacga
     4741 ggagcgtctg cttgcactga cgaaagagca taagctgatc gtgacactgg aagagaatgt
     4801 cctggaaggt ggattcggcg agcatgtatc agaattttac gaggagattg gaagcgatgt
     4861 gcaggtgatg aacattgccc tgccggatgc ttatatcgag catggaaacg tagaaatcct
     4921 taaaaaagaa tgcggtattg atgcagaaac gatagaaaag aaaataattg cggcagccat
     4981 gggactttaa aagcggctgc aaaaaaggag cacagatgaa agaaagactg gatgtgctgc
     5041 ttgtccagag agggcatgcg gcttcaagag agaaagcaaa agcggtgatc atggcaggct
     5101 gtgtctatgt caacggacag aaagaagaca aggccggatc gatgtttgac tgtgcggcag
     5161 agattgaagt gcgtggaaat acactgcgtt atgtcagccg tggcggtctg aaactggaaa
     5221 aagccatgaa aaatttcggc gtggaactgg aaggaaaggt ctgcatggat gtcggtgcat
     5281 cgacaggcgg atttacggac tgtatgcttc agaacggagc ggtaaaagtc tactctgtgg
     5341 atgtcggaca tggacagctg gactggaaac ttcgcaacga tgagcgtgtg gtctgcatgg
     5401 aaaagaccaa catccgctat gtgacaccgg aagatatcga agagccggtt gatttttcat
     5461 cgatcgatgt gtcttttatt tctctgacca aggtactgct tccggttcgg aatcttttaa
     5521 gccaggatgg gcagatcgtc tgtctgatca aaccacagtt cgaggcaggc agagaaaaag
     5581 tcggcaaaaa aggcgtggtg cgtgatccgg ccgttcacaa agaagtaatc gaaaaagtca
     5641 ttgcttttgc aaaagagcag tatctgcagc cgctggcact tgatttttca ccgatcaaag
     5701 gaccggaagg aaacattgag tatctgctct atctgcagaa aaagccggaa gggagcgaga
     5761 tgacagacgg tgacatggat gtggatgcag tcgttggaca ggcacacgga acgcttgata
     5821 aataaggctg gataaagaat ggacagattt tatgtaatat caaacagtat gaaagatccg
     5881 gaaggaaaga tcgcaaaaga gatcaaagcc tatctgaatt tcagaggaaa acagtgcatc
     5941 atacaggaac gccagggaaa agaaagcttg cgttcttata aatatacgaa tgcgaatctg
     6001 atcccgnnnn nnnnnnttgg cggggacggc acgcttttac aggcggccag agacatgatc
     6061 gagcggaata tcccgcttct cggagtcaat aaagggacac tcggctatct ggcagaggtg
     6121 gacggtgaca atatggaaga ggcactggac cggctgatcg atgacaacgt ggtgatcgaa
     6181 gaccggatga tgctcgaagg ctgtgcctac agccacaaga aaaagctgct gcaggatttt
     6241 gcactcaatg atatcgtgat cgcaagaagc ggaaggcttc agatcattga ctttaatatt
     6301 tatgtcaatg gcgagttttt acgttcttac agtgcggacg gcatcatcat ctctacgccg
     6361 accggatcaa ccggatacag cctctcggca ggtggaccga tcgtttcccc ggaggcttct
     6421 ctgatcctgc ttacgccaat cgcaccgcac acgcttaaca cacgaagcat cgtgcttccg
     6481 gcagatgcgg agatcacggt ggagctgctg cacggacata ccgcgggctg ggatgaggca
     6541 gccgcatcgt ttgacggaga tacctgtgtc aaactgggaa tcggtgactg tatccgcatt
     6601 taccgttcca gccgtatgac acgactgata aagataaaca acatcagttt tcttgaagtc
     6661 ctgagaaaca agatgaatga ccagtaggag gaaatgaagg gtgaagatca acagacacgc
     6721 aaagatcctg gatctgatca gcagagatga catagagaca caggaagagc tggcagaaaa
     6781 gctcaacgca gagggttttc cggtgacgca ggcaaccgtt tcccgtgata tccgggaaat
     6841 gaagctgaca acagtaccca acgccagagg ccgccaggtt tacaaagtga tgcagggtac
     6901 cgagacgggc atgaatgaaa aatatctccg tatcctgcgg gatggctttg tggcgatgga
     6961 tatggcacag aatatactgg tcgtaaagac ggtggcaggt atggcgatgg cagtggcagc
     7021 ggcacttgat gccatgaaat ggccggagat cgcaggctgt atcgcagggg acgatacaat
     7081 catgtgtgct gtccgcagcg tagatgagac actgatcgtc atggataaga taaaaaagat
     7141 cattgcagcc ggtacgccgc tgcataatta aaagaaatgg gagtgtaata tgctggcaaa
     7201 tattcatgtg aagaacatgg cactgatcaa agaggcagat atcagtctcg gaaagggact
     7261 gaatatctta agcggtgaga caggagcggg aaaatccatt ctgctcggct cgatcgccgt
     7321 tgcccttggt ttgcagaatt ttaaggggtt tgcaagggaa gatgcagatt atgcactttc
     7381 ggagcttgtt tttgtgattg aaaatgaaga acagcgacag gcacttctgg agctggagat
     7441 cccggtggaa gatggtgagg tgatcatcag ccggcggctg tcaaaggggc ggacgaccag
     7501 caagatcaat ggagagactg ttccggtttc catggtcaga aaagccggta cactgctgat
     7561 cgacatccat ggtcagcatg agaatcagtc tcttttacat attcgcaaac atctggaact
     7621 gctcgatgat tttgcaggag aaaagctggc tctggcaaaa gaaaaatata aagcccgcta
     7681 tcaggaatac ggcgaactga aaaaggagct ggatcaggca cagctggaca atgccaccag
     7741 agcaaaagag atggattttt taagttttga gattggtgag atcgaagaag ctgccctgca
     7801 aaaaggggaa gatgaggcac tggaggacga ttataagaga ctggcaaacg gaaaannnnn
     7861 nnnnnctgtg catccgacgg agcatcggaa gcgatcgggc gtgctcttcg aagccttgca
     7921 tccgtgtcag agtacgattc ggatctgaag gcactttata accagctgat ggatgtggac
     7981 agccttttaa atgattttaa ccgggagatc tccggatatg cagatgagct ggatgtcgat
     8041 gaagaaacgc tgaaaaagac agaggagcgg cttgatctga tcaaccatct gaagacgaaa
     8101 tacggtgata cgatcgagaa aatccaggca tactgtgaag aaaaaaagca gcgttttgca
     8161 gtacttgaaa actacgaagg atacctggcg gagcgaaaaa gccgttttgc cgcctgcaaa
     8221 aaacagctgc aaaaagaagc agaaaacctc actgtcatcc gaaaggaagc ggcacagcgt
     8281 tttacagaac aggtgaagca ggctctgatg gatctgaatt ttctggatgt acagtttgaa
     8341 ctcgatttcc gtctgctgga agattatatg gtatccgggc aggatgaagt gtgttttatg
     8401 atctccacca atccgggtca gccgctgcgt ccggtgcagg atacggcatc cggaggtgag
     8461 ctttccagga tcatgctggc ggtcaaatcc gtgatggcag accaggagaa ggttgagacg
     8521 ctgatctttg atgagatcga cagtggtatc agcggacgca ctgcacagat ggtatccgaa
     8581 aagctggcac tgatcgccgg aaaccatcag gtgatctgta tcacgcatct ggcacagatc
     8641 gcagcgatgg cagatgtgca ttttatgatc gaaaagaagg ttatcgaaaa cgagacccag
     8701 acctcgatcc ggcagctgga tgagaaagag agtatcgacg aactggcaag gatcctcggc
     8761 ggagcaaaga tcacgcnnnn nnnnnnttga cagcgcaaaa gagatgcgtc agctggcaaa
     8821 aaatgccaga ggacacggat tccagaatga gccagagtaa aagaatctga gtttcacata
     8881 ctagaaccaa atcaaaaatc agccgcagag gcagggaaag atgaggtgac cgtgtgtgaa
     8941 aaagaaaaaa ataacagaaa gaacaagggc aatctatcgc acgctggcga tgtttttgct
     9001 gttgttcttt tttcttgtga cgggtggatt ctggtatctg aaaagcagca tcccagatca
     9061 gctgaatgtg gcatcttatg aggatgtcga tacgataaag ctcccttttc aaagtatggt
     9121 aaaagatgag gttgtctatg catccgtttc tgcggaaaat acaaaaatcc cggaagaaaa
     9181 gatcaagatc cagtgttctt ttctgggatt cgttccgctc aaaacggttg aaatacagca
     9241 aaaagagctg ccgcaggtac tcccgggcgg caatacgatc ggtattttta tgaaaacaaa
     9301 agggattttg gttgtcggga ccgggagcgt gacagatgca gatggcatgg aaagagaacc
     9361 ttcccttgga atactgaaat cgggggatta tatcgtaaaa gcagatggaa aagaaatttt
     9421 cacaaaagaa gaaattctgg aaatcgtcga aaaaagtcag ggaaaaaaga tcagactcac
     9481 tgttataaga aatggattcg aaaaagaatg tcagattctg ccggttcgca tgcaggatgg
     9541 cagctgcaaa ctgggtgtgt gggtgcggga tgacacacag ggacttggca cgatgaccta
     9601 tgtggacggg cagaaacagt atggagcatt aggacacggg atcagtgatg tggacaccgg
     9661 gcttctgatg cagatccggg acggaaaact gtaccgggca agcgtgggag cggtaaagaa
     9721 aggagaaaaa ggcaatccgg gcgaactttc cgggacgatc tattaccggg atgaacagat
     9781 tctcgggacg atcacctcca attccgagac cgggatcaaa ggaatgttat atgccatgcc
     9841 gcaggaactg tccggccatt cgtttgtaca gacggcgtac cgccaggaag tgcatacggg
     9901 aaatgcttcg atctactgct caatcgacgg cagcgtaaaa gaatatacgg tggagatcga
     9961 aaaaatcaac ctgagccacc gcgatataaa caaaagcatg gtgatccggg tgaaggatca
    10021 ggatcttctt gaaaagaccg gcggcatcgt ccagggcatg agcggaagtc caatccttca
    10081 ggacggaaag ctgatcggag cagtcaccca tgtctttgtg gcagatccat cccgtggcta
    10141 tgcaattttt gcagaaaata tgctggagaa gtaagcgggg aggtatgcat ccggttttcg
    10201 cccatatacg acagaaaggt tgttctttta agcttaatgg aaggcagttt gtcgaaaaaa
    10261 gggagaaaaa tgcgacagga actgcttgcg tccaccaaaa gttctgctta taatacaagt
    10321 agtttttggc atatttgtaa ctaaatggag gtgcttttat ggataagctg aatatagcga
    10381 ttgcagatga caacgcgaag gttttacagc ttctggataa aatcgtgagc agtgatgaag
    10441 aactgaatgt agtcggaaaa gcaggaaatg gcgaagatct gtatgatatt atcaaaaagc
    10501 aggagccgga tgtcgtactt ctggatctgg tcatgcccaa agtcgatggg ctgactgtca
    10561 tggagcgggt gaacaaagat caggaaatcc gcaaacatcc ggcattcatc gtcattacag
    10621 ccgtcggcca ggagaagatc acggaagatg catttgacct cggagcagat tactatattc
    10681 tgaaaccatt tgacaatgag atggttatca accgaatcaa gcacacaaga gtaaagaaag
    10741 accgtaattt cgcaaaagta cagaaggtaa atgcctacga gagcaagaaa gaatacatgg
    10801 aacgcaacct ggagacggat gtgaccaaca tcatccatga aatcggcgta ccggcacata
    10861 tcaaaggtta tcagtatctg cgtgattcga tcatcatgtc ggtgagcgat atggaaatgc
    10921 tcaattccat cacaaagatc ctctatccga cgatcgcaaa aaaacaccag accacgccaa
    10981 gccgtgtgga gcgggcgatc cgtcatgcga tcgaggtagc gtggagcaga ggtaagatgg
    11041 atacgatcga agaattattt ggttatacgg tcagcaacgg caaaggcaag ccgaccaact
    11101 cggagtttat tgctctgatc gcggataaga tccgcctgga atacaagaat cgatgaaaac
    11161 acagatggga caagatggaa gctttatcag agaaaaaata taaaaattgt gcaaaatata
    11221 cagataacac ttttcaatag agaaaaaatg cgttataatg aaaaaaagcg gtaccgatta
    11281 ggaggaaaag tttatgaaaa atgtgttatt tgtagcatca gaatgtgtac cttttattaa
    11341 gacaggtgga ctggcagatg tagtgggatc gctgccgaaa tgttttaata aagacgagtt
    11401 tgacgtacgc gtgatcatac cgacgtatat gtgtatgaaa gaggaatata aaaatcagtt
    11461 agagtacata acgcattt
//
LOCUS       DAWDWH010000042        11355 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3022_length_11355_cov_13.651947, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000042 DAWDWH010000000
VERSION     DAWDWH010000042.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11355)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11355
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3022_length_11355_cov_13.651947"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3088 a         2592 c         2220 g         3455 t
ORIGIN      
        1 tcatacactt tcatcgtagc acagagacga ctgctcttat caatgtaaag tgccgccaga
       61 cactcttccc ccggctgcac acgctttgtc tgctctttat atggaagaaa cagatccttt
      121 tccagtcccc agtccagaaa tgcaccgatt tttcctgtat cagcgacgcg aagcacggct
      181 gtttctccca ggcggacttt cggcgtacgt gtcgttgcaa tcagccgatc ctgtgaatct
      241 ttatataaaa atacttctaa tatatctcct gccttgatgc cttccggaac ttctttcttt
      301 ggaagcagta ctttttcagt cgcattttcc tcttctccga gataaattcc aaatcctact
      361 tcttttacta cataaagttc ctggctttct cctaaatgaa acatattctt tctcctcgtt
      421 ttttgtattc ctgtttattt gttctgaaat tcgtaactgt tcagtacctt cacagttacg
      481 agtcaaaatg catttaaaat caccttcgtt aatggcattt tgacctgcat gtctcgggat
      541 tttggcatat tcatgccaaa acacctcgcg ggatactacc tactgaatag ttactgaaat
      601 tctaccactg catacggttt ctgtgcctca tcacacagca acaccgtaca ttttttctct
      661 tctatatgaa tataaggcac aaccatatca ccgagaagtg ctgccttttt atactctgca
      721 cgcatctggg aaatccggaa atcatccggc agaagctcca tcgccatctg cacatattgt
      781 ccattattga catgatgatt tgtatcaatg tgatgtttca cgatcagaaa gtactctttc
      841 ttttccattt cttccggaag ggcgatcttt cgcggtgcat aatccatatc cagcttctcg
      901 tgcagcacat acgcttcctg ctcctctttc ggaactctgg tcggatgtcc ggtctttaaa
      961 tccacgtaag tccacaatgt attcgcccag gcaagctttt cacctttttc atcccggatg
     1021 cagaaatttc tttcgccgag aaatccctta aaagaatatg gccaggtctg aacggtgatc
     1081 tcctgtccaa acaccggcag ctccccaagc acgatctgcc aggacgacag aacccatgca
     1141 cactgctttt ccttcagata atcaatcccg aagcctatct tttccgaatg aaaactgctg
     1201 cagtcctgaa aataatttaa tatagaaaga agtgtgatcc tgcgattctc atccacttcg
     1261 ctgtaacgaa ctctgctgtc aaactgataa atcatacgtt cctcccgaat cttgtggcat
     1321 cttcaaaacc aaacatcctg ttttttgaac gcagagaagc atttccccgc ttcaagtgtg
     1381 cagagcacta agcggtaggt gagggggatg ctatcaaacg gatgtcctga ttttcatttg
     1441 cctggtaact cattttattt tctgtatatg cagaaaaggt tgtcttagca gacaaccttc
     1501 ttaactgggc tacaaggatt cgaaccttga aatgacggag tcagagtccg ttgccttacc
     1561 atttggcgat agcccatcat tattttactc tgttgcgttt ttgttgctca caacaatgag
     1621 aattatatag taacgttcat aaaatgtcaa gcactttttt taactttttt aaatcttttt
     1681 ttattttccc ttttcttcca gcccgtgatg tgttataaca ggaagaaaag aaccccaatg
     1741 tatgcagaat ggaggaaact atgagtacta tgaatcttac cctgctgacc gatttctatg
     1801 aattaacgat gatgcagggc tattttaaaa cacagaatac cgagaaagta gtttttgatg
     1861 ctttttaccg caaaaatcca tgcgacggtg gctatgcgat cgctgccgga ctggatcagg
     1921 taattgatta tattaaaaat cttcgtttca gcaaggagga tatcgcttat ctgagaagcc
     1981 tgaacacgtt tgatgaagat tttctgaact atctggctga tttccgtttc agcggtgata
     2041 tttatgcgat tccggaaggc accgttgttt tcccacgtga gccgctagtc aaggtggttg
     2101 ccccgatcat ggaggcacag ctggtagaga ctgccattct gacgatcatc aatcaccaga
     2161 gtctgattgc tacaaaagct tcccgtgttg tctgtgctgc aaaaggtgat ggcatcatgg
     2221 agtttggcct gcgtcgtgca cagggtccgg atgccggtgt ttatggtgca agagctgcca
     2281 tgatcggcgg atgcatcggc acttccaatg tacttgccgg acagcttttt gatgttccgg
     2341 taaagggtac gcatgcacac agctggatca tgagtttccc ggatgaatac actgcattta
     2401 aaacatatgc aaatatgtat ccggatgcct gctgtctgct ggttgatact tacgatacgt
     2461 taaaatccgg cgtgccaaat gcaatccgtg tttttgaaga aatgcgaaat gccggtattc
     2521 cgctgaaaaa ttacggaatc cgtctcgaca gcggtgacct cgcttatatg tccaaacagg
     2581 caagaaaact gctggatgcc gccggcttcg atgatgctta tatttctgct tccagtgatc
     2641 tggatgagta tctgatcgag agtttaaaag ctcagggatg taaaatcact tcctggggtg
     2701 tcggaaccaa catgatcact tccagggatt gccctgcttt cggcggtgta tacaaacttg
     2761 ctgccgtaga agacaaagaa accggagaat ttattccgaa gatcaagctt tctgagaaca
     2821 ctgaaaagat caccaatcct ggaaacaaaa cgatctaccg tatttacagc aaaacgaccg
     2881 gaaagatccg tgccgatctg atctgtcttg ccgacgaaac gtttgacgag agtaaagata
     2941 tgatcatttt cgacccgcag gaaacctgga aaaagacaaa agtacgtgca aatacctata
     3001 cacttcgtga gctgcttgtt ccggtgttta aaaacggcga atgtgtttat gattctccaa
     3061 gcgtgatgaa aatccgtgat tacagcaaaa aagagctcga tactatctgg gatgaaacaa
     3121 aacgtttcgc aaatccgcag catgtctatg tagacctttc agacaagctc tacaagatca
     3181 aatcggatct gctcgaaaaa atgagcctgg aagctctgga atactaagat ggcataaaag
     3241 agaaatcccc ttcatagact gttctcagta caggaagggg attttttatg ttttcttatg
     3301 aaatgcttgt caactgtgaa aacagactgc ctgaagattt tgtgccgcca tcactggctg
     3361 caccggatat tgcatttgcc gcagatcctt cggacgagaa gcgttttctg gaagtccggg
     3421 ctgccagaca cgcaaaacgg ctttttgacg ccgccaaagc agaggggatt tttctctatg
     3481 gcatctccgg ataccgcagc tacgaacgtc aggaaatact ctatcagaag gcctgctgca
     3541 aatcttctgc cgcttcatct ctatgcgaaa ctgccatgcc tggatgcagc gaacatcaga
     3601 ccggactggc ccttgatgta tcctgcccgg ccgtctcttt gaatctttcg caggcttttt
     3661 cagaaacagc agaagggcga tggcttgccc gccatgcccc tctttatggt tttatcatac
     3721 gttatcccaa aggcaaagaa catctgacaa agatcccggc ggaaccatgg cacatccggt
     3781 atgtcaccag aagccttgct ctgtgcctga cactcaccgg gcaggttctg gaagaatact
     3841 acgcctgctg tgaacggtaa aatttaaatc cgaccagtgt tgtgagcact tccgtcactg
     3901 gaaaggtcag ccagaaatac tgcagtccaa acctggaaaa cagatatcca agcggaacaa
     3961 acaaaaccat tgtcctcaca acggtaagaa tcgaactttt cacggcactg ccgatcgcct
     4021 gaaaatacac cggaaaaatc agtgaagtga ccatcgggat aaatgcaact ccaatataat
     4081 gaaatccgtt cgttccgatt gcaatcacct gtgcatcttt cgaaaaggtt gaaagcattt
     4141 gaaccggcag aaattcaaaa cacagcgttc caagaaacat cagagccatt cccgcgatca
     4201 gggattcttt cagcacggct ttgcatctct cctgatcttt tgccgcataa ttgaagctga
     4261 tgaccggtac gatgcaggtc tgcaatgctc cgagtggaat aaagaaaaaa ctctgccatt
     4321 tgtaatacag cccaagtgcc gtcaccgcct gatccgaaaa tcctgcgagg atcatattca
     4381 gcccgaaaat ataaaacgtg tacgcagact gcatgagaat gttcggtatc ccaagctgat
     4441 aaatcctttt gatgtgcggc agataacagg atttttccgg tgatctcaca aaagcttttc
     4501 tcatgaccac aagagccgct acgatctgcc cgatcaccgt ggcgatcgct gcaccggcga
     4561 ttccgaattt atgtatgcca aacatcccga aaatcaggat cggatcaaga ataatattcg
     4621 tgattgctcc ggcgatctgt gccaccatcg gcgttttcat atctccgcgt gcctgcagga
     4681 ttttgctcca gactccttct gtaaacagtc cgatactgac cacgcagacg attcttccat
     4741 atacgacaac atctcgtatg atttcttctg aatttgtcga aatcctcgca taaaacggca
     4801 tgatcagata acagactgct gcaaaaacaa tccatagaaa aactgccagc ggcgtgccta
     4861 tgcctgccac ttcttctgct tttttctcct ggcggattcc gaaataatat gccatgtcgg
     4921 tgttgatccc gacgcccgtc cccaccgcaa gggcgatcat taacagctga agcggataaa
     4981 taatggacag tgccgtcaga cccgtctccg aatatttgcc cacaaacagg ctgtccacta
     5041 tgttataaaa tgcctggatt agctgtgcca gcatgacagg cggtgcgatt tttaataaaa
     5101 tgcggctgat tttttctttt ccgtaaaatt ctgatgtgtc catgctccac tatctctctt
     5161 ccatatttct tacataaact gtgcttcatg ccgcaccttt ttttcaatat ctgagaagct
     5221 ctcttcgtta tatcacactt accataccat ttcaagtcga acgcacgtga gacttggcac
     5281 gcgattctgg tcagctttgc tgacatttac ccaacagaat cgctgtgcat tctcgcggag
     5341 cgtttatctt agtcggagat tgcactccac taagacaaat ttgttgctct ccacttataa
     5401 aagtgggagc cttctccgac taaggtaaat agttaaacac agacaaaaag acgcatgaaa
     5461 aaaatttcgt ttttttcaca cgccttcgcc tttactgttc catttgtctt cccataaatc
     5521 ctgccgcaca tttcgcaagt gcgttttcgg tttcgcccat tttgacgctt cggtcttttc
     5581 cggagaggat taccgagccg atcgcatcgc ctccgcagag gattggtgtg atcacctgcg
     5641 gtacatccgg tttgctttcc ccattggtga gttctacatg acgctccggc gttttcccgg
     5701 cgagaatgga ttctctttcg ttaatcatat tttcaagcac atcgctgatt gttttgccga
     5761 tatattcttt tttcatgccg cctgctgccg ctacgatctg atcgcggtcc gtgatgcata
     5821 cgccgtgtcc gctcgtctgt gcaagtgatt ctgcaaattc tgcggcaaat tttccaagtt
     5881 ctccgacctg agaatatttt tttaaaatta cttctccttc gcggtctgtg aaaatctcca
     5941 gtggatcgcc gccacataaa acgttgacac cgaaagcctt gtccctttgg ttacgcacct
     6001 tttggactat tgtggcttct aagctactct cgatgtccca cttaaaattt acgacttttc
     6061 ctctgtaacc ggctttttca agctcgtcct ccgttaatgt tcccgattca agcagcattt
     6121 gaatatcgga ctttaactga atgtcaattt tcgattcgtc gccgtcgtga atgattattt
     6181 tatcaataat aattgacaaa tcgcctttgt caagcttttt cttattgaca atatctctga
     6241 aaatctcaat agcgtttttg gcaattcgat taacctcaat tgtttgattt cggcgttcaa
     6301 ccattaaagc cgtctgcttt tccaagcctt caatttttga tatgagttcg ttttccatat
     6361 cctcataagc ttcctcaatc atatctcgct gctcttcgtc ggctttcata atatctctga
     6421 ttttttgctt ttgcgttgcc ttgtattcct cttttgccct tgccaaatct acctgcaaat
     6481 ccgcaacgct gtctgcattt gatttcaaat cgtccgcttc gtttgcgata ttcttttcga
     6541 gctcggcaat catcttgtcg gaggttttca taattcgctc aacatatttt ttcaaaacgg
     6601 tatcaagcac atcaactctg atatgatgag aggtacaacc tttaagccca cgcttatgat
     6661 aagttccgca ggtatatgcc ggcgccaaat cgggtcggct cattgaaaac atcggacttc
     6721 cgcactcgcc gcaaaacaag aatccggaat agtcgttatc gtattttttc gttccacggt
     6781 aattgttttt tgtccttgtt ttaagctgct gctgtgcttt cataaattct cttgtggaaa
     6841 ctatcggtgt atgatgattt tcaaaaacta tgttttcctc agcgttgagc cttttatcgg
     6901 gtccgttaat tcgcttgcgt gtatattttc gctggcgaag tgtgccgata taaaaatcgt
     6961 tttgcaaaat tccgctgacg gtcggtatag cccaaacagg cgaagcctta agcttaatat
     7021 caaccttttt cttatttaat tccgccatct tacgcttgcg ttcgatttca accattcgag
     7081 gggttggaat tttttcgtct gtcaaggtgt ttgctatttt cttgtatccc attccgccgt
     7141 tatacatttc aaaaatcctg cgaacaacct ctgcacaggc ttcgtcaacc tcgtaggtca
     7201 tatttttgct gtcaatcata tagtagccgt acggaacggc acaaatccaa tcggcgttat
     7261 tctgacgcat ttcaacaacc tttttaacct ttttggaagc gtcggtaaca ggcatttcgt
     7321 ttagtaaaaa tcgcacctga atattattcc aatcgtcata tgtcggataa tctattccgt
     7381 ctccgattga tataatacgc actcctgcat cacgcaaatc ctcaagttca acaagtccct
     7441 tgctgttcct acgggaaaaa cgggacaagt ccttaatcaa aagaatatcg taatcaccac
     7501 tcataagcaa cgggcgcatt ttcatatagg attcacgctg ctcaaaggta taacccgaac
     7561 ggtctcggtc aacataataa tcaacttctg cgtcgggaaa tctttgcttt aagtacgctt
     7621 ttataattct tctttggttt tcaatagagg tattatctct gtctttttcg gtatcaaccg
     7681 aaatacgcat atatgccgct atcttcttac ccaaaacatc accaccaata ttatatttga
     7741 tttatgttct gctttatgaa caacattgta acatacttgt atatagaaat caataaaatt
     7801 tgaagaatgt ttatgaaagt taatattatg tacggcagaa ataattctgc catttgaacg
     7861 agcttatcgt tccaagtctt tacttcgttt gcgagctcgt tctcttgcca taacatctct
     7921 gttatgaagc aataattccg ttgtattttg gataaggtct tccgttccgg acaaaaagtt
     7981 tactacctta tatccttttt ggttccactg cctatattcc ggtctcaaag actctcgaag
     8041 ctccttgtcg tctttttctt cctttgaaaa ccaataatag gctatcttta ccttgtcatc
     8101 tacttccata aacataagtt aaacctccgt ttattccata tttttcctca aatttattgt
     8161 ttctattcag tcattgtttt ttacctgccg gagaagaaag ctatttccac agcaaactgc
     8221 tcttttcggg atacaggtat ttcttttcaa tttcaagctg aaatttgttt aatttatcaa
     8281 ttttgtcttt caacagtctt gtgtcaacaa aaccgagtga atttgctttg attgctattt
     8341 gatttaggtt gttgctcatt gcgtaaaact gacgcattaa gtcgtaaaat ctgtcatcgg
     8401 gtttttctct cggctcataa cccttaatca gtattcttaa cacggcactc tgagacagtc
     8461 cgctaagttc aacctttttc ataaaatcgg cattttcttt ttctgaaagc caaaactgtt
     8521 tttttattct tttcatagaa ctcctttcta atcttagaga cagctatatt taggagatag
     8581 atatattttt tgcggcaggt aaatatcttg ctcctttagt attacttcac tctgttttat
     8641 cgacttttga aaatccgaaa agccgatttc aaaacttttt gagataaata atatttgctt
     8701 ttccctgtcc ctgcctgttt cgctgtatca aatccgcttt ttcaagctcg ccgaacagtc
     8761 ttattatttt gtcgtgaccg aaatgcagca gctcctgacc ttgttttatt gtgaagaatt
     8821 gatacactct gccgtaccta tcaacccagc cgtttcttgc ggataaatac attcggtcaa
     8881 gcagcaatgc gtacagcatt tttgcgtcgg tagaaaccga acagtatttt tggtcggtaa
     8941 acaatgcttt cgggatttat agaaaacata tttttccgct tcgtttttct taaagtattt
     9001 gttcaaatta agcctccttc tttctgtttt gaaaagatgc atttttacgc tgtttttagt
     9061 ttgctttcat ctttcttatg gcgggtaata tatttccctg tcttttgttt ttagccttgt
     9121 tattcgtgcc ataataaaaa ctcctttcac tcatttgtac aaaagggtgt attttggaca
     9181 cccaaaattg ccgatttaac gaataatttt ttgaaaaata ttttttaccc ctctacttgt
     9241 ttgaaccggg agtgcctttt tgcaccccca taagtcggaa attttttcaa aaacccttca
     9301 cttgtttgaa atgggagcag cattttttgg gagtgttttt taaaaagttc ataatctttt
     9361 aacatttagt cccttcactc atttggacaa agggtgccga aatgcacacc caaaatcgtc
     9421 gatttaataa aaaatttgca aaaaaataag aagccttttt ctcaagcaag aaagaagctt
     9481 ctaattatta aagaatttat accctaagag gtatagtttt cttttttaag taaacaatat
     9541 acccaatagg gcttattata acaaatattt tattgacatt gcaccctaaa gggtgtagaa
     9601 taagcataga taataactac aaggagcagt aatatgaacc cgattgctga atttgtaaaa
     9661 gaaaacagaa aagcggcagg acttacgcag gagcaatttg cattgcgttc gggtcttggg
     9721 cttcgctttg tgcgtgagct tgagcagggc aaggaaaccg tgcgtatgga taaggtaaat
     9781 gttgcccttg caatgttcaa cgcacaggct gttgccggaa agaaggaaga taaatgagcg
     9841 tattcagaac tgcctatgtt tatgtgagaa acacctttgc cggcgtcctc aaggaaacgg
     9901 acaacggcta ttctttcaaa tatgatgccg attatttaca gagcgaaaat gcgtctgccg
     9961 tttcactcac cctgcctttg caagcggaag aatacacatc aaaaactttg ttttcgtttt
    10021 ttgacggcct tattcccgaa ggctggcttt tgaaaattgt aaccgataat tggaaaattg
    10081 acagccacga ccgcttcggc gttttgctcg tagcctgcaa ggacggaatc ggcaatgttt
    10141 caataaggga ggaaaaagaa tgaactgttt atgctgcggc aaacctttgc gcacacccga
    10201 tgaaacaggc tggcacaagg cttgcattaa acgctttttc ggcacaacca agctccccga
    10261 aattgaaatt gacgacaaaa cgcttaatct tttggcaaca gaaacgacaa ataaaggctt
    10321 taccgttccg ggagtgcaaa agaaattatc attgcacctt gtcagcgaca gccgcaagcc
    10381 gagattaaca cttgtaaact atccgaccgg ctatattctc aagccgcagg ttgcagagtt
    10441 tgaagcatta cccgaatcag agcaactcat aatgacaatg gcggatatgg cggggatttc
    10501 gaccgttccg cacgcactca ttaaaggcaa tgcagggctt gcatacatca caaagcgagt
    10561 tgaccgcaat ttaacaagcg acaaggtaga aatgctcgct atggaagatt tttgccagct
    10621 tgatttaagg cttaccgagg ataaatacag aggctcatac gagcgttgcg cgaaaataat
    10681 caagcagtat tcatcgaggg tcggcattga tatggcagag ttttacatta ggcttgtatt
    10741 ttgctttatt gtcggcaatt ccgatatgca cctaaagaat ttttcactta ttgaaacagc
    10801 cgagggttcg ggcgaatatg tgctgtcccc tgcttacgat ttactgccgg tcaatgcaaa
    10861 tatgcctgcc gataaagaag aattcgcact tgcaatgaac ggtaaaaaaa tgaatatacg
    10921 caaaggcgat tttcttaaat atgccgacac ctgcggcata tctcgccaaa ctgccgaaaa
    10981 gcttattgaa aatcttgtta ggcttacgcc aaagtggctt aaaatgtgcg ataaatcatt
    11041 gctcccggat gacttaaaag agagattgac aaagattatt actgaaagaa caggctgttt
    11101 gattagtaaa taacagtgaa tatcacaact atcaaacatt attcggcgta aaaaagtgta
    11161 gccgtctaac actttttcgt tgtaattttt gtaaagcggt gtatttagta tcatatatat
    11221 attgaatgct atagagcagt ccgattagtg agcaaacaga aatattaaaa taaatggaga
    11281 aatgattatg gcaagaacaa ctaaaccaaa gaaagaaata tcaatggaag aagcactttg
    11341 gaaaagtgct gataa
//
LOCUS       DAWDWH010000043        11291 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3034_length_11291_cov_11.147383, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000043 DAWDWH010000000
VERSION     DAWDWH010000043.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 11291)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11291)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11291
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3034_length_11291_cov_11.147383"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2356..2365
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4046..4055
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3442 a         2157 c         2931 g         2741 t
ORIGIN      
        1 acccaaggtg ctacttcaca attgacgtga atggcggttt gctttttacg gagagtttat
       61 agcttcgatt acataaatta cttaatcaga aaatatgtat ggaggaactc gcttatgcta
      121 acaatcggtg acttgattgc agtaattagt ctctgcttaa ccagttttgg tcttggctat
      181 acgattggtg cgaatagcaa taagaccgaa aaaaaataac cgccccagtc tgcaaactaa
      241 gcggttattt ttaacctaat aaacatcagg cgaaccgtca tggtagcacc tttttgtatt
      301 taaatatgaa ctgtctgtat catagcacaa aacgactttt gtgtcaaatt atcagcagaa
      361 tcttaagcgg ctattataag cgattcatca gatctttctg attacaacag atgtcccgtt
      421 tgcaatgact ccgattacat tattggaaaa tatattgtag tcaatgtcaa tgccaatgac
      481 tgcatttcca cccaatgcag ctgatttttt gatcagttta tccatggcag cgtgccttgc
      541 tgtctcgagc ttttcctcga atatcccgga ctgcacgcca aagaaatcag caaacgaaga
      601 agtaaattct gaaagaaatc cggtgccaag aactacttcg ccggcgacaa tccccaggta
      661 agatacgatt tcataacctt caaactgaaa tccggttgtt accatgtggt gtttttcgcg
      721 ataatccaga tctttttttt cgttttctgc ccgttgtatt tcttcctttt ttgcacgact
      781 gtaccataat ttttcatcat aatccggatt gccatatcga taacctgcac gcatcgcttc
      841 gatgatctga ggcaattctg ttggatcgat ctggcttagc tcttcatctg ataacagttg
      901 aaccatttca tttccacaat aatcgcagtt ttctgcaaga tgattggttt ctctgcaatt
      961 tccgcagtgt ttgcagcata aaatcataag cgttcctcct ttagcgtatg tattttcaaa
     1021 acggtaagct cttaagttaa ataccattat aataggaatc tgccatctgt acaatgtttt
     1081 tcttttgtga acaggaactg aacctgtcct tttttgtgaa atatgtgttt tcaggctgaa
     1141 tttacgggat ttgtcggatt cctttcataa ctttcttgtg agaaaaacca cttcgtgtta
     1201 aaatatcaga agacatttca cgaaaataca acaagagggg gtggcggcgt gacgaacttt
     1261 gatttcctga aggatattcc gcagtttgca gcgtttgcgg atgttgcggt ggcggcggaa
     1321 aagatcctga tgatcgaccc ggaggcgagt gtgatgaact gccggcgggc gatggaattt
     1381 gccgtcaaat ggatgtattc ggttgacgat atgctggtga tgccatatca ggaccagctg
     1441 gtcagcctgc tgaatacgga agagtttaaa ggaattgtgg atgcagacat tttgcggaga
     1501 atggatttta ttcgcagggt ggcaaatcag gtggcacata cgggaagaaa gctgacgctg
     1561 gatcaggcga agctgtgcct tgagaatctt tatgtatttc tggattttgt agcttattgt
     1621 tatgcggatg attatcagga ggggcagttt gatgcaggtc ttccggagca gaatcaggag
     1681 atcctggcag cggagacggt ctttccggat attgatctgg aggctttgat cgcagaaaat
     1741 aaagcattga aggcagaact gacttccagg cgtgaggagc agaagcagac gtatgttccg
     1801 aagccgctgg agctgtcgga gtatgcaacc cgcaaacaat atatcgatac gatgctgatt
     1861 gatgccggat ggatggaagg aaagaactgg ctgaatgaag tcgaagtcta tggcatgccc
     1921 aataaatcag gcagcggttt tgcggattat gtgctttatg acgatgcaca ccggccgctg
     1981 gcggtgatcg aggcgaaaaa gacctgtgca gatgtggcga gcggacggca gcaggcagag
     2041 ttatatgcgg attccctgga gcggcagtat ggacgcagac cggtcatttt ccttacgaac
     2101 ggatttgaga cgaggattgt ggacggacag tatccggagc ggcaggtttc ctgtatttat
     2161 tccaggaggg atctggaaaa atggtttaat ttaaaaacca tgcggggcga tcttggcagt
     2221 gtccggattg ataaaaaaat cgccggaagg tattatcagg aagaggcgat caaggcagta
     2281 tgcgaaagct ttgataaaaa gaacagaaga aaagcactgc ttgttatggc taccggatcg
     2341 ggaaagacaa ggacannnnn nnnnnttgcg atgtgcttct gcaaaatggc tgggtgaaaa
     2401 atattttgtt tcttgcagac cgcacttccc ttgtcacaca ggcaaaacga agctttgtca
     2461 atatgctgcc ggatctttcg gtggcaaatc tgtgtgagga gaaaagcaat ctgcatgcac
     2521 actgtatttt ttcaacttat cagacgatga tcaactgcat tgatgccaca cgggacgaag
     2581 acggaaaatt atttacgagc ggccattttg accttgtgat ctgtgacgag gcacatcgtt
     2641 cgatctacaa taaatacaaa gacattttca catattttga tgcaccgctt gttggtctta
     2701 ctgcgacgcc gaaagatgag atcgataaaa atacctacag catttttgag ctggagaagg
     2761 gcgtgccgac ttacggttat gagctggcac aggcggtaaa agacggttat ctggtggatt
     2821 tcctttcggt ggaaacaaag ctgaaatttc tggaagaggg cattgcgtac gatgagcttt
     2881 cggacgaaga taaagaagca tacgagaaca cctttgtgga tgaaaacgga caggttgtgg
     2941 agcggatcca gtcctcagca ctgaatacct ggatctttaa tgacgatacg atcagacagg
     3001 tgctggacat tctgatgaca catggaatcc atacagacta cggagaaaag ctcggcaaga
     3061 cgattatttt tgcgaaaaat catgctcatg cggaaaaaat atttgaggtg ttcaataaag
     3121 aatattcaca tctgccgggt tatgcaaaag tgatcgacaa tcgtatcagc cattcccaga
     3181 gcctgatcga tcagttttcg gagccggata aattgccgca gatcgccatt tccgttgata
     3241 tgctggatac gggaatcgat gtgccggaaa ttttaaatct ggtctttttt aaaaaggtaa
     3301 tgagcaaggc aaaattctgg cagatgatcg ggcgggggac aaggctctgt ccgggacttt
     3361 tggatggtga ggataagcag aaattctata tttttgattt ctgtgggaat tttgagtttt
     3421 tccggatgca gaagggaaaa gagacagcag tccggctgcc tttgcagagt gcgattttta
     3481 acctgaaatt tcagatttcg tataaattgc aggatttaca atatcagaca gaaagactga
     3541 ttgcctatcg caaaaacctg gttgagagta tgcagggaaa agtacaggaa ctgagccggg
     3601 ataattttgc ggtgcgtcag catttgaaat atgtggaata ttattccaat ccggagaatt
     3661 atcagaatat ccgttacgag gatacgctga ccgttcagga ggaggttgca ccgctgatca
     3721 tgccggatca ggaagaggcg tccgccgttc gttttgacgc actgctttat gggattgagc
     3781 ttgcttatct gatgggaaat aaatatggaa aagcgagaaa ggatcttctg aaaaaggtgc
     3841 gtgcggtggc aggtgttgcc aatattccgc agatcaaagc acaggaaaaa ctgctgaatc
     3901 agatcctgca cacggattat ctggagacgg ccggtgtgga tgaatttgaa catatccgtg
     3961 aaaatctgcg ggaactgatc aaatatatca aagaaaaaga aggaaagcgg tatgataccg
     4021 attttgcgga tgaacttctg gcgatnnnnn nnnnntcaga gctggaaaat gatgacctga
     4081 aaaattataa agcgaaagca gaatattatg tccgcaggca tcaggataat gaggcgattg
     4141 ccaggctgaa gaataatcag ccgctgacag cggcggatgt acagcttctg gaagagatcc
     4201 tctggggaga actggggaca aaagaagagt atgagacgga gtacgggcag aagccgctcg
     4261 gagaatttgt ccgtgaaatt gtcggcttgg atatgaacgc tgcaaaagaa gcattttcag
     4321 aatatctgga tgaaaacagc ctggacagca ggcagattta tttcgtaaat cagatcgtgg
     4381 aatacattgt gcaaaatggc atgatgaaag atctgtcggt gcttcaggag gcaccattta
     4441 cggatcaggg aagtgtcgtg gacattttta cggatctgaa cgtatggatg ggaatccgga
     4501 aagtgatcga ccagatcaat gcaaatgccg cagcagctta ggagataaat aaactatgaa
     4561 tttttttgat tacataaaga acgagtcgtt ttttaaaccg cttacgttca agtaccgcag
     4621 gatttattat gactgtatcc ggattttgat cgaacgttcg aaggaacttc cggttctcta
     4681 cgagtcggat gcaaaggaca gtatcacgct gtatctgaaa aacgaagaga ttcagcgact
     4741 gatcgaagaa gcggatgatg agaatgaggc ggtgcagctt caggcggctg agattctggc
     4801 cattttccgt gaatgtggat ggatcaagcc acggcagatc ggacgcagcg gagaatatgt
     4861 tgtaaatctt tcgacggatt gccgaagagt gattgatttt ctggataaac tgactgaaaa
     4921 gaaaaatgaa ggtacgatgt caaaccgcat tctctcaatg tatgagatca tgaaatccgc
     4981 atcggaagaa ggctcagtgc gaaaagaacg tccgtactca aacattctgg tgccgatgat
     5041 ggaaaatgaa accgagcttc gcaatgagct ggctgattta aaagacagta tatcggatat
     5101 catgaaaaca gtcatcgaat ttcaggacat gaacagtttc gggcagttca ttatgaaaaa
     5161 tgaaatgttg gaccggttct tcagtgaata tttcttcgta aaaaacaacg gactgatccc
     5221 gacccagctt tctttcatcc gtgaccggat cgaggatctg cgttatgggg atatgtatga
     5281 gaaaatggtc gaagaatgtg gaaaacgtct gcgttgcccg aaagagcagg caatttcccg
     5341 tgtggaccgt tacatggcgg aactccagta tttcctgagt gtggaatacg aagaaaatat
     5401 ggaactgatt gatactcgca tcaacagtta ttataatctg gcaaatacga ggatcatgca
     5461 gatggcgagc agccgtgtca gactggagaa tcttctcggc gatttcctgg accgtgtttc
     5521 caggatttca aaagaagagc aggatgcggc gatggaaaaa gtggaaaaat gcactcgtgt
     5581 tatcaaccag aaatacgttg gttacaaatc attcgagcag aatcggcgta tccgcaacga
     5641 aggtcagaat atcgcattaa acaccaatgc gatgtcggaa gaagagatgc agaaacggac
     5701 ggagaatctg ttccgcagtg caccgaaccg atatgctccg gcccgtgtcg gagcatatct
     5761 ggatgaggca cttggattgg atgtgagcat ggagctgaaa gaaaagggtg tgcagacaaa
     5821 agaagaggca ctgatgatcg cagcggcgat gctttatgcg aaaaatgcag aatttccata
     5881 cgaagtccgc atcaaggacg aaaaagtgag cacgaaaatt gccgatatca gcaatgtggt
     5941 gatctcgaga aaataaagat gtcagaattg tggatcacag ggtacgaaaa tatttacagg
     6001 gcattttttt gaacgcagag aagcggattg tgaatgtccg atttacaaat acaatgaggt
     6061 aaatttatat gcaggattta agttttataa gcaatatgag cgacgaggat gcgtatctgt
     6121 tcaaacgctg tgtgcggcgg cttctggata cgacatttct tgtaaaagac aaagaagaaa
     6181 agctctatca gtttgtggct tccgaatcta accgctacga catgaaccag tatctggcag
     6241 tgatcggcta caaagtggtt gtggaagaac agatgggtgt ggcgatgctg cagccaaatg
     6301 atgatgataa agaaacggtt gggctgaaac gattgaacct ttatcatttt gaccagaggc
     6361 acatgaagct gctgctggtg ctgtggcttt tatttctgga gcggatggga tatgaagagg
     6421 cagtctatgt gacggtcggt gagattatcg acaaatgcag gctttatgat attgagctga
     6481 cgccggtcga gtttaaaaag gcatatgcga ccttccgcag attctgcctg atcgattaca
     6541 gtgacgatat cacaaaagag gacggggctg tgcgtctcta tccttctttg cagttctgtc
     6601 tggatatcgg acagttgaaa caggtgatgg cagaatttgt ggaacaggaa gatttcgagg
     6661 cagcggacat ggcagacctg gaagaggcag aaagtgagga gagcgatgag taaaatagtt
     6721 ttacgcaatg tacagctgat caactggtat ggatttacca acacggtcgt tccggttgca
     6781 gaaaatctca ccctgatctc cggagaaaat gagtgcggaa aatcgacgat cctcgatgcc
     6841 atcaaatatg cctacaccgg tgatacgcag tttaataagg cgacaagcgg atataataca
     6901 ggcgtgggca agcgaaatct gatctcgtat acacgctgtc tggtcgatgc gagtgccggc
     6961 gtgtatgcaa gaccggcgga gaagatccca gttgtgtata cccacatcgc actggaatat
     7021 tatgaccaga tcaacgaaaa atcctttgtg ctcggcgtga tcatcgagac agccgtgacg
     7081 gatatccgcg gcacgtactg gtatgcgatg gaagacaaac atctgtcgga tatttcctat
     7141 gtctatgaag acggttctgc cttaaagccg tacgatgcgt ccggatttca gaaacgttac
     7201 aatgtcaaac tccgcaagaa acaggatgga gtggcattgt ttatgcagat ggtcggactg
     7261 aagcttccgt atcaggaagt gtttcgttat cagagaaaac ttcgaaatat catggcatac
     7321 aatccggcgg caaagatcca ggaatttatc cgggaatcgg ttctggaaga acacgatgtc
     7381 aattttgaaa aattaaaaga tgccaagaaa aatattgagc agatcaacag ccgtttggag
     7441 ctgatcgaag aagagatcgg tgatctggat gcgatcctga aagattatgc agaatacgat
     7501 gagcgtgtca tgcagctgaa agtggatgat attaaattaa aatatcgaaa tatgctttcc
     7561 tggaaagaaa aacgctgcat ggcagaagaa atgatccgca aaaacagcat tgaagtagaa
     7621 gcacagatca aaacaatcaa agaactgagc acggagatcg atgcacttga ccgttattat
     7681 atggaagcaa aagccgcact gcgggaactg aatgtttcca aagcgatcga caattccaga
     7741 cagcagatca ctgctttcga gaaacagctg gagaagcgga agagtgaaag ggaagagctg
     7801 gagcagttcc ggaaacgtct gctgcatgtg gcagaagaac tgaacaaaat ggaatgcgag
     7861 gtttctcatt ttgaaacgct tggcaaactg acggcagtcg atctttccaa ttctgagaaa
     7921 caggaagcca tcgatgcact gagagcggaa atccgtaaaa aacgtgacag cattgtgcga
     7981 aacagggctt atctggaccg tgagctcaaa gaagtgcagc tggaacgcag tgaacagagc
     8041 aagatcgttg aaaactgtga ccgcaacaaa gcggattatt cgcatgtgca ggaacagacg
     8101 cagctgatcc gggagatcaa caaagaactg aaaaaacagg gcatcgatga agaagcccgc
     8161 atggcgtgtg aatatgtatc ggaactgaaa gacgaagcat ggcgtgatgc gatcgaagct
     8221 tttcttggtg tgcaccgcta tgcgatcctg gtctcgccgc aggcctttga tgttgccaat
     8281 caggttatgg accgctccgg acatcgttat gtggagctgg tcaatacaaa gcgtctggct
     8341 gaacgcaaga tcaaatgtga ggaagattcg gtctatcatt atcttcagat ccagaatgag
     8401 acagcagcaa agtatttcca gttctggctg ggaacgatcc atgcagtcga gctggacgag
     8461 gttgcaaaat acgacaatgc catgtcaaaa gaaggaaaac tcggcagaaa tatggcagtt
     8521 acttacatca acacgaaaaa gatcaagagc tactgccttg gcggggcggc gattgagctg
     8581 aaccgcaaaa gagctgcgaa aaaactggat cagctggaaa aacgggaaaa agagattttg
     8641 agcagcagac atacgatgca gaatcaggat gtctttctgc agagcagtct ggattatttc
     8701 aaagattaca atctggaagc caacagagaa tgtgagcatt taaatgcaga gctggaagca
     8761 gaaaaggtgc gttatcgtga acttctggaa gcccagaaaa acaatgcaga atttatggca
     8821 ctttcgcagc gtgtgaccga tctggaaaaa cagcttcgca ataaaacacg ggaaaaagac
     8881 gaagagagca aaaagcgtta ccgactggaa gcggaaatcg aacagaaaaa gaaagacagc
     8941 gtgatgtatc aggcgaaaga agcggaagag cagaaacatc tggaagaaga acaaaagctc
     9001 aaccgttccg cagcagagca ggcgatcaca gaatacgacc gctatcttgc cggagaagat
     9061 aaaaaaggcg gcatcatgca gctttccgag aaagagcaga tggaaaaacg tgtcagcagt
     9121 tttgcggcaa atatcaacgg acagcagcag gcatacaaca accgcaagca ggaagaagaa
     9181 cgtctgccga tcggtcttga gtacgaaaaa agctatcacc agagacgcgg aaaaatctgg
     9241 atcgatgacc ttcagaatct gcagcagaaa ttaaaagagc agaccatgcg ttatgaaagc
     9301 attttcaaaa gagaatttgt tctgaatatt tacgagacgg caagggctgc aagaaaagac
     9361 atcgcacaga tcaacaaaga acttcgcaag ctgaaattcg cgacatgcta tcagtttgat
     9421 gtgaaatttt tgaatgacaa ttccgactat gccaaaattc tgcgttatgc agagtatctt
     9481 cagcagaccg gaaatctgga ggacggtcag atcgccctta caaagctgat gggttatgag
     9541 gatgacgaag tagagagacg ggagcgggag atcaaagata ttattaaccg catcattgaa
     9601 aaaaatgatc tggcggagat ccagaaattt gcagattacc gcaattatat gagctacgag
     9661 atcatcatca acaacgaaga agtaaaagac ggaaaattat ccaaacaggt aggatacaat
     9721 tccggtgccg gaacgcagat cccatatacg ctgattttgt cggcggcact ctccatgctc
     9781 tacaatgtga gagtcaattc cgtaagactg atctttattg atgagccgtt cgaaaaaatg
     9841 agtgaccaca atatcaaact gatgcttgat ttcttcaaaa accaggactt tcaggttatc
     9901 ttctgtgcac cgccaaataa actggaatcg atcggaagtg aatgcggtgt gatcatcccg
     9961 gttttgaaaa atgcaaacga cgatatgttg atcggaaagg tgaaatttca tgacacagtt
    10021 aatggagcaa attaaagaaa aagatgcaca ggcatttacc cacagcggaa agtttcatgc
    10081 ggacgatgta ttttcgtctg cacttctgct gtctgtcaat ccagagatca cgatccaccg
    10141 cggcagtcag gtgccggagg attttaaagg aattgttttt gacatcggaa gagggaaata
    10201 cgaccatcat cagagggata gcagaatccg tgaaaatggt gtgccgtatg cagctttcgg
    10261 acttttatgg gaagagttgg gtgctgagat tttaggagaa gaactggcag aaaaatttga
    10321 cgaggctttt gtgcagccac tggacaacaa tgacaacacc ggcgaaaaaa atgaacttgc
    10381 caccttgatc ggcaatttca atccgccatg ggatgcacag ggcggaaacg atgcggcgtt
    10441 ctttcaggcg gtcggtgtgg ccggcatgat tctcgataac aaactggagc gttatcgggg
    10501 caatgagcgt gccgacaaac gtgtagaaga aattttaaaa cagcatcagg aagagatcgc
    10561 ttccggcaaa aagccggcgg atgaaaagca gattctggtg ttgccggatt atgttccgtg
    10621 ccagaaatat ctttccgaga cagacattgc attcgtgatc tttccatcca atcgcggcgg
    10681 ctactgtatt cagcctcaga agcgagaata ctccctgaat tataaatgca gtttcccgga
    10741 aagctggctt ggtcttgaaa aagaagaatt aaaagaagag agcggtcttt ccggtgcaga
    10801 attctgtcat aagggcggat ttcttatgac gacaagtacg ctcgaagatg ccataaaggc
    10861 atgcaagatc agtcagcagc agttccatga gcaggttgtg atcgtaaatc ttggcacaga
    10921 tgaggcagcc gatgcattgc ttttcaaact tccgcagttt tccggtgcca gaatcgtgca
    10981 catggagttg ccggatcttc cgccgatgga gtgggacggc atttatggtg aatccacact
    11041 tgaaaagcag gaatggaaag cacagatcaa agatcaggtg aaaaaaattc tgaaatccaa
    11101 accggaagca gtctatgtgc agggagatct ttttgcggtg tatccgctga tccatgcact
    11161 tcgtaaacgc catatgccgg tgcttgtgat gacagagatg gaaggggaac agaaaattgt
    11221 gcggattccg tccggatcat agtgtgattt cagatttttg accctggcat taagatgcct
    11281 gcgaattgct c
//
LOCUS       DAWDWH010000044        11163 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3071_length_11163_cov_12.628106, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000044 DAWDWH010000000
VERSION     DAWDWH010000044.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 11163)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11163)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11163
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3071_length_11163_cov_12.628106"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4532..4541
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10231..10240
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2469 a         2833 c         2234 g         3607 t
ORIGIN      
        1 cccgacctcg tcattggaac gcacacgtac acggcggtcc agctgtccct ggctgatcgc
       61 accggctgtc tctcttaagt cacgcagcgg ttttaacatt cttcgtatca tccagaacac
      121 cgcattgatc gccaccgcaa cgattgcacc caaaatgatc aggaacatgc cgatctgcgt
      181 cagcacattg ttataaattt ccgaaatatc ccgcaccagc agaagctgat acgatgtatt
      241 tttactgtct gaaagcggga ttttctgtcc gacgatcatc agatgtctgc cgtttttctg
      301 ctgaaaagct gccaccggct cataagaatc aaactgttca agccggtttc cggtcagctc
      361 gaaattcgtg gtattcgctg caagctgcca gtcctgaagc agcatataat ctctgctgct
      421 gtatctgcgc agcaaaaact ggagataagc tttccttgcg gtgtcgctca tctcctccgt
      481 agctgcattt tcaagactct gtcccagtgc ataagcggtc gcacgcacct gtcgtgacgc
      541 tgcacttgat gtctgcctga tgttataatt tgatgtctca tacactgtga tcccaccgta
      601 aactaccatg gagatcaaaa gaatcgctgc cgtgagcaga gatattttct gccagagctt
      661 catactactc ctccagtcga tatccgatct tcggaatggt cttgatgaca tccgacagat
      721 ccagtttcct gcgaagctgt ccgatatgta catcgatcgt ccttgtctca cccagataat
      781 tgctgcccca tacttcattc agcatctgtt ctctggtaaa tgccacattt ttattctttg
      841 ccagtaatac cagcagatca tattccatcg gcttcagact gactgtctgc ccgtttctgg
      901 tcactttatg ctctgataaa tggatctcca aatctttcac ctgtatgatc tctttttgct
      961 ttccggtacg ctccagcact ttttccacac gcaccagcag ctccagaacc tcaaacggtt
     1021 tgacgatata atcctctgct cccgatctca gtcccttgat cttggaattt acatctgcct
     1081 tagccgtgat ataaatgacc ggtatgccat attttttcat aactccgaga agttcaaagc
     1141 cgtctttgcc cggcagcata atatccagca gtgccaggtc aaaatcctcc tctttctcca
     1201 gtgttccctc aacttcattg ccatccagaa ccggaaccac ttcgtagccg gtcacttcca
     1261 gattcataca gatcagctct gaaatcgccg tatcatcctc taccacaaga atgcggtttg
     1321 ccataattct ctgcccccgt acttctacat tttataactg cccgccatcg gtgcagttac
     1381 cctgctttca ttctttccgg tcatcctccg cttccaggct cttcagttcc cggcagagtc
     1441 tggaaatcgg aagtccgacc acgttataat aatctccttc aattctttct acgaaaattc
     1501 ctgcacgtcc ctgtattcca taactgcctg ccttgtcaaa cggctccccg gtggcaatgt
     1561 acgcttcgat ctcctgatca ctcatcgatg tcataaagac atcggtcctt tcatggaatg
     1621 accggatgat cttttcatcc ccttttttct gtaacagtgt cacgcccgta taaacctgat
     1681 gcttctctcc ctgaagcatg gcaagcatct gatgtgcatc ttcggcatct tttggttttc
     1741 caagtacttt gtttcctttg gcaacgatcg tatctgcccc gatgatcaca gcatctcctg
     1801 tcacggcttc tgcaactccc tttgcttttg caagtgacag ttcttcaacg atctctcccg
     1861 gttcttcttt tgtgatacat tctctggtac tgccgatcag tatctcaaaa tcgatcccgg
     1921 cctgttctaa aagctcacgc cttctcggcg aagcggatgc aagtacaatt ctcatacagt
     1981 cctcctgtcg cggtgctgcc tgcggcaacc caaaagaacc acccctcgct attactataa
     2041 caaagagcgg ttcttaaaat cttctaattt ttgtaaacaa aatgtaattt ccgttagcgg
     2101 attaccttaa tccatccttc cggtgcttcc agacgaccca tctggatacc ggtcagtgtc
     2161 tcatacagtt ttttggtaac cggtcccatc tctgtcatac cgcttggcat gcagatttct
     2221 ttgccgtgat ctacgatctt tccaaccggt gagataacag cggctgttcc gcaaagtcca
     2281 cattctgcga aatcttttac ttcatcgaag taaacttctc tttcttctac ttccagtccc
     2341 agatattctt ttgcaacata aacaagagaa cggcgtgtga tagaaggcag aatactgtct
     2401 gacttcggag ttacgacctt gttgtctttt gtaacgaaca ggaagtttgc tccgcctgtc
     2461 tcttctactt tggttcttgt agccggatcc agatacatgt tctcgtcaaa tccctgacgg
     2521 tgtgcatcca tgatcgcatg caggctcatg gcatagttca gacctgcttt gatatgtccg
     2581 gttcctcttg gtgctgcacg gtcgaaatcg ctgacacgga tcgtgatcgg ctttacgccg
     2641 cccttgaagt acggtccgac cggtgtacag aatgcacgga actggtattc atccgccggt
     2701 tttacaccga taactgcatt tgttccgaac atgtacgggc ggatatataa agtagcacct
     2761 gagccgtatg gcggtacata atctgcattg gcttctacca gttttacgat tgcatctacg
     2821 aaacgatctt tcgggaatac cggcatttcc agtctttttg cggaattctc catacgctct
     2881 gcgttgaggt ccgggcggaa tacaacgatg cgtccgtctt ctgttgtata tgctttcata
     2941 ccttcgaata ccgtctgtgc atactgtaat acgcaggcac actcactgat tgtaaccatg
     3001 tcatcggtac ttaatgtacc ttcatcccag gcaccgtttt taaaatttga tacatagcga
     3061 tagtctgttt ttacatagcc aaatccaaga tttgaccagt caatattttt tcccatgtta
     3121 tcctcctgac gccttacctg ctgctttttc aatccataaa tactggtaca acagtctgac
     3181 aaaatatttt tgatatgttt ctaagtataa tacttttttc tgtgatgcac aagcatattt
     3241 cctgcaattt ttagcaaatt catgcaaaaa ctgcaagaat caggtccgtt tgataaattt
     3301 actgcggcag gaaggacagg tgatctcaat ttttcccttc cctctcggga cacggatttt
     3361 ctgtctgcaa tgcggacagc ggtagatttt gtgtgttttg gaatcgctga tcacactttt
     3421 gtcccctttg cagaaccgga taaccttctc ttttgtgtcc atatacatac gctcttcttc
     3481 gcatcgtttc cggatatttt tggaaaacat gcggtaatac atccagatca gcagaatcca
     3541 gctgacatag ttcagtgccg atgcccgcag cagaccggaa agcaggatgc ataccagtgc
     3601 tcccaccagt aaaaacctgg aaaaatggtc gattccatat ctgccctgca taaatttttc
     3661 gaatttctgt ctcatactat accctttctt ctgacccggc ctttcttttg cccttatctt
     3721 tcttttacac cgctctgatc cgcctgaggc tatatcagaa aacgcgatgg gtcatacgtg
     3781 atttttgtct cttgactcat cttaatactc cgcccgcaga aaatcaataa actggctatt
     3841 aaataattct aaagttgcat ccctttctta acatcgtaaa gccgagtgca tgtgagacgt
     3901 ctcactgctg ctccaatatt tctccaaagt tccgccgaag tctcttgcca gtgtccgtat
     3961 gttttgttat aatgcagtta gttttgtcaa actatgtcgc ccgatcgggc gacggaatgg
     4021 agttttgtat gaaagtaaaa catatgacgt ttctggcaat gtacacgacc attgccctga
     4081 cgatttttgt ggtggaggca atgctgccgc cgctcgcccc gatccccggg atcaagctgg
     4141 gacttgccaa tgtcgttacg ctgtggcttt tgtattatgc tacctggaag gatgctctgt
     4201 gcgtacttct gatgcgtatc ctgatcgcca gcatggtgac cgggcagatg gtatccttct
     4261 cctacagcct gtgcggcggt ctgttctgtt tcgtggccat ggcactgctc tttcggctgc
     4321 tcgggaaaaa gcacatccct tttatcagtg tgatcggtgc ccttttccat aacctcggtc
     4381 agatctgtat cgccatggtg atccttcgct cggcaagtat tcttgtttat ctgccgatgc
     4441 tgaccatcag cggaattctc accggagcat tcaccggact gtgtgcatgg tttgcatcca
     4501 gaaggcttcg aaaagaccag gtgtggttta tnnnnnnnnn ncacggcttt cagagacata
     4561 catgcctgga tatcctgaac tgctgtaaca aaaacagagt cccgaaaacg ggactctgtt
     4621 tttgttttgc agcttttcat cctggatgat gcctgcgaat cgccctgaca caaaggacaa
     4681 ttgcatcctt attttgtttt cttataagat gccttcttta cgcctgcttt tttcagagct
     4741 tttttcagct tgttcagctg tgattttttc atcttcttcg gtactttcac cgttactttt
     4801 gattttccgg acttgaatgc attctttcca acgcccggtg ctttgattcc cttgaaggtg
     4861 atcgttgcca gtttcttgca tttgtagaag ctgctcttgc caatgctctt gacatttgct
     4921 ccgatcacaa cgctcttcag tctggatgca tttttgaata cattgtttgc aactgcggtt
     4981 actttaaagg tgtatccgtc aatggtcacc gttgccggaa tcgttacagt cttgaccatt
     5041 ttcttcagaa gcttgcttac tgttaccgta ccattcttag catctgattt tgttactttg
     5101 aatgccagtt tgccgtcagc actcttatga acagatcctt ttgccggaac tttcttcggt
     5161 acatctggtt tttctgtctc cggagccttt gtctcggtct gcggagcttt tgtttctgtt
     5221 tcgctgtccg gttctttggt ctctagctcg ttatcctttg cctttacgtt gatctcaacc
     5281 gttgctttca catccggtct tgccgcactg cttacggtaa tcactgcttt tcctgctgcc
     5341 tttgcggtta ccacacctgc atcactgact tctgcgactg ctttatcact ggattcatat
     5401 ttcagatctt tgctgtctgt agtatcttcc ggagtgatca ctgcggtcag ttttgcagtt
     5461 tcgccgactt tcagttcgct cttgtctgct tttacagaaa ctcctgtgat tggtttttcc
     5521 acaaccggtt caccgtttac ttttactgcg atttctgcat atacatcgct gtcttttaca
     5581 gaaactttga ttttggtctc accggcttta actgctgtta cattgccttt gtcatctacc
     5641 gttgctatgg tttcatcttc ggatgcaaag gtaagctcgc cttttgcata atatggtgcg
     5701 attgctgcac ttacctgtgc accttctgcc tctttcagtt ccagagcatc tgtcgaaaca
     5761 tgtacacccg ttgcagacat atcgatgtta ttgtatttat actcatcgaa gtcataccac
     5821 agtaccgtct tttcattatc tgctttgtaa gttccgttat ccagatcatc tttgctcagt
     5881 gctgctgagt aaacgcggat gttcttcaga tatccggaag aaagacgtct tgtctccgga
     5941 caatatccga taccaagcgg ataggtcgtt gtagctacgt ttcccacatt ctgtgtggtt
     6001 accatttctt ttccttctac ataaacagaa atattgtttc catcgtaaac tgctgctaca
     6061 tgcagccact ggttcatttc ttctgaagta agaggaacct gtacggtctt ccagcttcca
     6121 ttcgtattct tgataaagaa gtacaccgtc tgctcagaaa cacggaatgc cgcactgctg
     6181 tcaccttttg atgcgatcat attgtagtca gcaccattgt atcctacatt attcggatta
     6241 aatacagctt caattgtaaa ggctttattt cccccgatca gactgttgaa ggtttcatct
     6301 gctgtttctc cggttacatc tgcataccct ttcagactaa tttcgccgtc tttttctgca
     6361 atatttgctg tagctgctgc actgatatcc atatcataat gatttgcact cttgtcttgt
     6421 acaacaaccg ctgcatcttc cgtctctgca aatttctttg ccagtgcgat tgcctcctgc
     6481 agtttctgat aacggagttc acctaccaga gccttacctt cttctgccag tgcattgtaa
     6541 gatacgagca tgttctgaag ttctgttgca tcactggctc tctgtacata aagttcatca
     6601 atttcatcaa tcttagcatt cagttcttct gctgctgctt tgatgatatc atcctcagaa
     6661 acagatgcta cggtgcggta tgcacgtgtt acatccatcg gatcgctgtc ttttgtggta
     6721 tatggtacca tggtgtactc ataagtataa gctttgttat ttggaagcag gtatgcggaa
     6781 agtgtatccg ctccgcagct cttgtttccg gttccctgtg aacgatagtt tactgtcagg
     6841 atcgtggaat ccagtttggt cagttcatat ggatgctgtg cctgatccag atcatctact
     6901 gtgaaatgca gtgcagacgc ttctaccgta tcggttgctg cgattgccat tgcactcttt
     6961 gcagatgggt tggttacgct gatccatttt actccggtta cggtaccggt atcctgtgta
     7021 tccaggtacg gatagaacat tccggatacg gtgttgctat atcttccaac cgttgcgaaa
     7081 tcttctctgt cccacatagc ctctaccggg ccgttaccat accattctac gttttcatat
     7141 ccttccggca gttccataac agtaccgata cggatataac gtccaaggct tgttccggtt
     7201 gcgtctacgg ttgcatctac agtcactgca ccgcttccgt ctaccgtata aaccatggtc
     7261 tgtttcaggt ttgcctggct tgcacttgcc agtgtcacac ggatcgtctt ctgtcctgca
     7321 tcgtttgttc cgaccgcaat atcagaagcc gttacatttt tgttgacatt cttccagttt
     7381 ccgtcgtagt taccgttatc attgttgaga atacctctcc agtaattcgg aaccggtccg
     7441 cttgtcagca gagtctcacc tttgtataca taatttttca gtgttccggt tgctttttct
     7501 acttcaaagc tgaaatcggt accggaaact ttgatcacat cttctgcact gtcatctacg
     7561 gttacgtttg tgttgatcgt cggttctact tttgtaactt ctgccgggat ctggaactgc
     7621 tcgtaagcaa cttcgtgtcc tgcttttgcc cattcggtat cctctttaag ctgtacagac
     7681 aggttcagat aatactctgc tcctgctttc ttttcttccg gcatagatgc acgatacgga
     7741 actttgatgc ttccgctctc tctgcctgcg atgttggtat cttccgcaga aagtttaccg
     7801 gagccaatct cttttccgtc ttccgtcagt gtccatttca cgtcaaaatc attcagattc
     7861 aggaagttat tttcattgta aacatcgatg gtctcgccga gcagcctgct gtcatctgcg
     7921 gtaaaccata cgctctgata ctgatatttt acttcataaa gttccggctg gacatccctg
     7981 tccggagata ccagaccgtt tacacagaag gagttgtcgt tcgggctttc gccggagtct
     8041 ccgccgtatc catagaagct gcctttgatt tcatcggagt acagatttgc atgagcatcg
     8101 gtatctgcat agtaatcata cagtttggaa gattcatcaa cggtcaggcc cgaaaaatct
     8161 gcccacagaa gtacatcatc agatttctct gtgattgccg gtgtcgtgct gttctgagca
     8221 ttgatctcct ccagggacag tgctctgctg taaacgcggc ccatagaaat ttcaccgtca
     8281 aaggttctgc cgttgtcagc actgcatcct actcccagtg ctacgctgct ggatgcaatg
     8341 gttgtatttc cgtttccgct tccaaccagc tctccatcac agtagatctt gatcgcacct
     8401 ttatcgtaag ttgcaacaac ctgatgccag tttcctaccc agttatccgg ttttttcgct
     8461 gtcacagaat tccagttgtt gttatagtaa gcaaagaact ccagctgttt actgctgttt
     8521 gtctttaatg caaactgatg gtcacctttt gcgatcagga ctttatctgc tccgtccgat
     8581 gccggtttgc agataacttc tacggtaaaa ctcttgccgc ttccggagag tgcttcgttg
     8641 tatttatcgc tctcaaacag tgcataacca tttgcactct tgccggtcag tgctccgctg
     8701 tccggatttt catcgacaga agaaatgctt gcagatccta ccacgccgtc tttcttttct
     8761 gttacaacgt aagactgtgg cagattgtca aggcttaaga ttctggactg gtcaacccaa
     8821 tcccagataa atccgccgag catattgtcg gaagaacgga tcgcatccca gtattctttc
     8881 aggttaccaa ccgcattacc catcgcatgg tcatattcac acagaacata tggcatattt
     8941 gctgctgctc tggaatacag ggtatcaaca cttgggtaca tattgcttcg catatcggtt
     9001 ccattgtttc catccgaaga ttctgaatga atcggtcttg tactgtcgtt gttcttgaag
     9061 taccagatca gatcatagaa catgccattt gcataagaag cactgctgct gtaataattt
     9121 tcgtttccgg ttgaccacat aacgatggca gtgcggtttt tcagtctgtt gaatgcggta
     9181 accgtacgat ccattgccag gttcttgaag tttacctgtt tttctccctg attcatcagt
     9241 gcatgagatt ccaggttggt ctcacccatc acgtaaatac cgtatttatc acacagataa
     9301 tacagatact cgtcgttgct gtagtgagag gtacgcagtg catttaagtt gtactgtttc
     9361 atcagtttga tatcttcttc ctgtgtctca tgcggtacat atttgccgta ttccggatcg
     9421 gtatcatgac ggtttgtacc tttgagcaga agctgtttac cgttgatcgt gatcggtttg
     9481 tactcagaat cggttgtgat acgattacca tttgtatcta cttctgattt tgtaaactcg
     9541 atctcacgga atccaagctg ctgggaaacg cttcccatgt aggtgcctgt cttggaatcg
     9601 tacagggaca gaaccatcgt gtacagattc ggcgtctcag cagaccacag ttccggtgaa
     9661 agaaccagtt tgctgccagc tgtcgaaacg ctgccgtcgg tatcaccgtc tgctgccgga
     9721 acggtatcca gatccatcgt catatcgttg acaaacatct ttccgtcctg atcataaaga
     9781 cgtacatcca ctttataacc ttctgctgct gccttggaag catttgctac ggttacattc
     9841 agcttcatcg ttgcattttc gtagttctca tcgagatctg tctctacttt atagtcctgg
     9901 atatgtacca gcggtgcaga atacagatat acatcacgga aaataccgcc atcataatac
     9961 atatcctgat cttccatcca ggtaccgtca cagaatttgt gaacttcaac tgccagaagg
    10021 ttgtcgcttc cgtctgttgt cagataatcg gtaatatcaa agctatgtgg actgtagctg
    10081 tcctcgctgt atcctacttc tttgccattt acatatacat aataggaaga ttctacgccc
    10141 tggaaggaaa gataaactct tccgtttgct gccttcatgt catcggtcac cttaaaagtc
    10201 ttgcggtaaa gacctaccgg gttataattt nnnnnnnnnn ttgactgcca aggcatagtt
    10261 acattggtat agatagaaaa atcaaatccc tgtctcgtcc agctgcaagg aagctgcaga
    10321 tttgttttcc agtcgttcgt cgttgctttg tagtcggtct tatagaaatc tttgtatgca
    10381 tcaccctgtg cgagtgcctg gttctgtaac acaacaagtg accagtctgc ctgatccttc
    10441 cctgttaaga actgtacata ttttgatgct gctttgtcgt aatcttttgc acctttgatt
    10501 gctttctcta cgctgtcata caccacattg gtcgttgcaa aagtgcttgc ctgttctgtg
    10561 ttgatgccat atacatctgc tgctttgacc tgattgccat ccacatcggt ataggtttct
    10621 ccagtccatt ctttgtgcgt gaattttgtt ttgtccacgt cggatgttac ttttgcggcc
    10681 tgatttggga cagctgtttt tgtccacggg tcggtgtagc tggcattggt gttggactta
    10741 ctgtctgcct gcgtctgcag tgcagactcg gatgctgctt cagctgcagg tgctgcactt
    10801 actgcctgtc ctgcgatcat ggaagccgcc agggaaaatg ccataacttt tctccacttc
    10861 atacttcttg ctcctttctt tttgcatctt ttgaagctgc agtctgcagc ttccttctaa
    10921 tacatgctct aacaaaaatt tcagtccgct ttcaccggac ttagtaaaat tttaccatac
    10981 tattcacaat ttatcaatag tatctctata tattttacag tgttttacca gatttttcac
    11041 taaacttttc actattttca gtaatttaca caaaaaaagt gtgacatttt tgtcacactt
    11101 ttttcttgca agatttcgct aaaagttgaa tgtgcaaacg aacggtatcg taaaaaataa
    11161 tac
//
LOCUS       DAWDWH010000045        10868 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3154_length_10868_cov_10.654120, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000045 DAWDWH010000000
VERSION     DAWDWH010000045.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10868)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10868)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10868
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3154_length_10868_cov_10.654120"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1223..1232
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5206..5215
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6895..6904
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9064..9073
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3429 a         2140 c         2911 g         2348 t
ORIGIN      
        1 tgacctggat gtggatgagc tggtctacag cgagattctg gtgaactggc cgcttcgtgt
       61 gctgtgcaaa gaggactgta aaggaatctg cagtatatgc ggtaagaatc tgaatcatgg
      121 gacctgcgac tgtgatcata cagatttaga tcccagaatg gcacagatcc gtgatatctt
      181 caataaattt aaggaggtgt aagccatgtc aatctgtcca aagaataaat cttccaaagg
      241 aagaagagac aagagaagag caaactggaa aatgactgct ccgaatctgg taaaatgcag
      301 caaatgtggt gaactgatga tgtctcacag agtttgcaaa aactgcggaa catataacaa
      361 aaaagaaatc gttgcagttg aagactaatt gcataaatgc tatagaatca gggattgaac
      421 aatttgatgt tgtttggtcc ctgatttttt atgaaaggat ctgcaaaaat tttaggttga
      481 tttttatatc gcactctagt atattaggaa ttgcaacagg aggaaaaact atgcaggata
      541 tggtcagggt ggttgtagat gccatgggcg gagacaacgc acccggagaa atcgtcaaag
      601 gtgccgtaga agcactgaac gccagagaag atatttttat ctacctgacg ggaagaaagc
      661 cggtgatcga agccgagctt tcaaaataca cctttccaaa agaaaggctg gagatcgtgc
      721 ataccgaaga agtgatcgaa acggccgaac cgcctgtcat ggcaattcgc aggaaaaaag
      781 attcttcgat cgtaaaaggt atgaatatgg tcaagcagaa agaagcagat gcgtttgttt
      841 ctgcaggaag cagcggagcg atccttgtag gaggtcaggt gatcgttggc aggatcaaag
      901 gaattgaaag accgccgctg gcaccgctta ttccgacaaa gaacggagtt tctctcctga
      961 tcgactgtgg ggcaaacgtg gatgccagac cttcccatct ggtgcagttt gcaaagatcg
     1021 gttccatcta catggaaaat gttatgggag tcaaaaatcc gagagtcggg ctggtcaaca
     1081 tcggtgcgga agaggaaaag ggcaatgcac ttgtaaaaga gacgtttcca ttgctcaaag
     1141 aatgcaaaga gctcaatttt atcggaagtg tggaggcacg cgacattccg cagggagtgt
     1201 gcgatgtgat cgtctgtgag gcnnnnnnnn nnctgtgagg cgttcgtggg aaacgtgatc
     1261 ctgaaacttt acgaaggtgt cggtgcaact ctgatcagta aagtcaagca gggcatgatg
     1321 acaagcctgc gttccaaaat cggtgcatta ctggtaaaac ctgcattgaa agaaacgctc
     1381 aaatcttttg atgccagcca gtatggcgga gcaccgcttt taggtttgaa cggactggtc
     1441 gtaaagacac acggaagttc aaaggcagtg gaagtgaaaa attccatcct tcagtgtgtc
     1501 cagttcacaa aacaggagat caatcagaaa atcagagaaa atattataaa agaagaccaa
     1561 aaaggataag agaggtattt tattatggaa tttgaaaagt tagcaaaaat catcgcagaa
     1621 gtgttaaacg tagatgcaaa cgaaattacg ctggatacca cctttgtaga tgaacttggt
     1681 gcagattccc tggatgtttt ccagattatc atggggattg aagaagaatt cgatatcgag
     1741 attccaaatg aagaagcaga aaagatcgtt tctgtcggtg atgcagtaga agcaatcaaa
     1801 agtgcattaa attaaaaggg gtctgacgat aacgacagat gggaaggtgc tgcaagtcag
     1861 cactttctgt atatccgact gaaacagaaa gcgaggaaca agatgaaaca gacaaaagat
     1921 ttaaaagagc tggaaggaaa gattggctat accttcaaag ataagacctt gtttaagcag
     1981 gctctcacac acagttctta tgcaaacgaa cacaggcacg aaggactcaa agacaatgaa
     2041 cgcctggagt ttcttggcga tgcggttctg gagatcatca gcagtgagta tttattttac
     2101 aattacccgg atatggctga gggcgaaatg acaaaactcc gtgccagcat tgtatgtgaa
     2161 ccgacgctgg cactgtgtac gcacgagatc agcctcggaa gctatctttt ccttggcaag
     2221 ggagaagagc ggacaggcgg aagaaaccgg gattctatcg tatctgatgc tatggagagt
     2281 gtaataggag ctatctatct ggatggtggt tttgctagtg caaaggagtt tatccataaa
     2341 tttattctaa aggatatcga acataaaaaa ctcttttatg atagcaagac tattctgcag
     2401 gagatcgtgc agagtgacta taaaggcaaa gagatcgagt atgtgctgac aggagagatc
     2461 ggaccggatc atgacaagaa atttgtggta agtctggtag tgggagacaa gacactgggc
     2521 gaaggaacgg gaaggaccaa gaaagccgca gagcaggaag ccgcttaccg tgccattatc
     2581 aaactgaaag gaaatgcctg agacatgtat ttaaagagtc ttgagatcca gggattcaaa
     2641 tcatttgcaa acaagattga ttttaaattt cataatggta ttaccgggat cgtaggacca
     2701 aacggaagcg gaaagagcaa cgtaggagat gcggttcgct gggtacttgg tgagcagagt
     2761 gccagacagc tgcgaggaag caacatgcag gatgtgattt tttccggtac ggaaaatcga
     2821 aaaccgctcg gctttgcgtc ggtttcgatc acactggaca acgccgacca caaactgccg
     2881 gtggattatg aggaagtgac ggtcagccgc cgcgtctacc gctccggcga gagcgaatat
     2941 cgtttaaacg gaacttcctg ccgtctgaaa gatattcagg agattttcta cgacaccggt
     3001 atcggaaaag agggttactc catcatcggc cagggacaga ttgaaaagat cttaagcggc
     3061 agaccggaag atcgaagaga attgtttgat gaggcagccg ggattgtcaa gttcaaacga
     3121 agaaaagcgg cagccgtgaa aaagctggag gatgaaagag cgaaccttgt ccgcgtaaac
     3181 gatattctct cggaacttca gcgtcagctg gttccgcttc agcatcaggc agaacaggca
     3241 aagatctacc tgaagaaaaa agaagaatta aaaaagatcg atctgcatct atttctgctg
     3301 gaaatggagc agcggaaagc agaacttgcg aatctggatg aaaaacgaaa gattgcagaa
     3361 gaacagcttt ctgatataaa gaaagaatac gagacgatcc gcatccatta tgaaacagaa
     3421 gaaaaagcga tcgaggggct ggaagagcgg atcgagcaga tccgggaaca ggagcggcaa
     3481 agtcagttgc tccaccagca gctcaccggt cagatcgaag ttctgaaaga gcagatccgg
     3541 gcacaggagg atcaggaagt ccgcttaaaa gagcgaaaga gcgtgatcga gagcgaactt
     3601 tccagaaaag aaaaacagag aaaagcggaa gaacaggcgt ttttgcttgc aaaggaaaaa
     3661 caggagcagg tttctttgac ggaagcggac tatcgcctgg aagcggatgc gacaacggcg
     3721 atgctctccc atctggaaat gcagatcgag gacgggaaaa atgaaatcat ttcacttttg
     3781 aatcagcgtg cttcggtgaa aggaaatgtt cagcgatacg ataccatgca ggaacagatg
     3841 cagatccgaa aagcagaagt aaccggaaag ctccttcgga tcggcagcga agatgccaga
     3901 tggcaggaga cgcttcagga atatcagact gcctacgaaa cggtgtcgga agagattgaa
     3961 aaacagcttc ggaagagcaa agagcaggaa gataagattg aacagctcca gcagaaactg
     4021 gcagggcaga accgggaact ggaacagaaa cagtctttct atcaccggga gaaatcaagg
     4081 cttgagacac tgagcaattt aacagaacgc tatgatggat acggaaacag tatccggaag
     4141 gtgatggagc agaaagataa aaatccgggg atcctcggcg ttgtagccga tctgatcaaa
     4201 gtagaatctt cttatgagac ggcaatcgag acggcacttg gcggcagtat acagaatatt
     4261 gtgacggaca gggaagagac cgcaaaacgc atgatcgaat ttctgaaacg tggcaaatat
     4321 ggccgggcta ccttccttcc gctgactgcc atcggaagaa aaggaaatgc aggaaacaaa
     4381 gatgccgtga aagagccggg gattttgggc gttgcgagca ctctggtccg gacagaagag
     4441 aaataccgtg cactggtgga ataccttctt ggcaggacac ttgtcgcaga caatatggat
     4501 catgcgatcg cagctgccag gaaatatcac cattctctgc ggatcgttac gctggaagga
     4561 gagtctttaa gtccgggcgg ttccatgaca ggtggtgcct accgcaacag cagcaatctt
     4621 ctgggacgca gaagagaagt tgaagagctg caaaagaaag tacaggcact ggaaaaagaa
     4681 tggaaagaat tgcagctgga agtcgatgaa aaccgaaaaa aaaggaatga acatcgcgaa
     4741 acagttgtaa ttctgaatga aaagcttcag aaagcctatg tttctcagaa tacggcacgc
     4801 atggagatcc gccagatgga agaaaaacgg gaagaatcca aagaagagct gacaaggctt
     4861 caggaagaat ctgcccagtt agacggcgaa ttaaagcaga tcagccggga aaaagaacag
     4921 atcgaagaag ctgtgaaaga gtctgaaaaa agagaagcac agctgcagga acagatcctg
     4981 ggatggcaga aagaacagga agtccagagg gcggaactgg agcagcagaa cgcccgcatt
     5041 gaaaagctgc atctggagct tgcaaatgcc ggacagaaag aagaatttgc agggcagaat
     5101 ctcaaacgta tcgaaaaaga aatccgaaat ttaagggaag aagaaaaaga gatcgggcag
     5161 aaaaagcggg aaagtctgga tgtcattgca gaaaaagaag cacagnnnnn nnnnncagat
     5221 tgaaagccgc attgcaggta cgaaagacca tggacgcgat catggcaggc agctgcagaa
     5281 gcttcaggca caaaaagaag agaaaagtgc ggcacagaaa acctttttta caaggcggga
     5341 ggagctttcc gggcagatcg cagatctgga caaagaaaac ttccggcttg catccatgca
     5401 ggaaaagctt gaaagtcaaa aagaagagcg gatcagtcag atctgggatg aatatcaggt
     5461 gacaccttcg gaagctgctg cgttccggaa cgaaaacggc atggatcaga agcagaccag
     5521 aaaacggatc gaggagatca aagcagcgat ccgccagatg ggcaacatca acgtcaatgc
     5581 gatcgaagat tacaaagaga tgtcacagcg gcatggattt cttcagaatc agcacgatga
     5641 tctcatcaaa gcagagaaag ctttagaaga gatcattctg gagctggatg aagggatgcg
     5701 gaagcagttc cgggagaatt ttgccaggat ccaggtggaa tttgacaaag cttttcgtga
     5761 attgttcgga gggggcaaag gaacgctgga gcttgtggat gatgaagata ttctggaggc
     5821 aggcatccgg atcatctctc agccgccggg caaaaaactg cagaatatga tgcagctctc
     5881 cggaggcgag aaggccttaa cggcgatcgc cctgcttttt gctatccagc acctcaaacc
     5941 gtccccgttc tgtcttttgg atgagattga ggcggcactg gatgaggcga atgtcagccg
     6001 ctatgcaaat tatctgcaca agttgacaaa aaatacacaa tttattatta ttacacacag
     6061 acgcggaacg atggcagcgg ccgaccggct gtatggcatc acgatgcagg aaaaaggaat
     6121 ctctgcactg gtatcggtaa atctgatcga agaggcactg gatcagtgag aaaggaaaga
     6181 agatgggtga gaaaaaagga ttttttaagc gtctggtaga aggactgacc aagacaagaa
     6241 acaatatcgt ttccggtatt gattctattt tcagcggatt ttccgcaata gatgaggatt
     6301 tttatgagga gatcgaagaa atcctcatca tgggagatct cgggatcaat gcgacaaccg
     6361 cgatcataga agatctgaaa cagaaggtga aagagcagca catcaaagag ccttctgcct
     6421 gcaaacagct tctgatcgac agcatcaaag cccagatgaa tgtcggtgag acggcgtacg
     6481 agtttgaaaa ccgtcagtcc gttgttctgg tgatcggagt caacggtgtc ggtaagacaa
     6541 ccagcatcgg aaaactggca ggaaaattaa aagaccaggg aaagaaagta attctggcgg
     6601 cagccgatac gttccgtgcg gcagccggag aacagctgac cgaatgggca caccgtgcag
     6661 gagtggagat catttccgga caggaaggat cggatccggc agccgttgta tacgatgcga
     6721 tccaggcatc caaagcacgt catgcagatg tattgctctg cgatacggca ggacgacttc
     6781 ataataaaaa gaatcttatg gcagagcttg gaaaaatcaa ccgtgtgatc gaccgggaat
     6841 atccggaagc ttaccgcgag acactggttg tgctggacgg tacaaccggt cagannnnnn
     6901 nnnnttgctg atattaccgg aatcattctt accaaaatgg atggaacagc aaagggcggt
     6961 atcgccgttg ccatccattc tgaacttggt atcccggtga aatatattgg cgtgggagag
     7021 agtattgacg atctgcagaa attcgatgca gacgagttcg taaatgcact ttttgatgtg
     7081 aaagattcag aagaagagga gtaaagacat gcttacatta gacaaatttg aagaagcttc
     7141 agaaatcgta aaaaaagcaa cacaggagac aaagctgatc tacagtaaat atttcagtga
     7201 gcagacagga aacaaagttt atctgaaacc ggaaaatatg cagacaaccg gtgcttataa
     7261 agtaagaggt gcttactata aaatcagcac actgagcgaa gaagagagac agaaaggtct
     7321 gatcacagcc tccgcaggaa accatgcaca gggcgttgcc tatgcagcaa aagagtttgg
     7381 tgcaaaggca gtgatcgtta tgccgacgac cacaccgctg atcaaagtaa accgcaccaa
     7441 gggctacggt gcagaagtgg ttctctatgg agatgtctat gatgaggcct gtgcctatgc
     7501 actggaactg gcagaaaaag aaggctatac ctttatccat ccgttcgatg atccggctgt
     7561 tgcaaccggt cagggaacga tcgcaatgga gatcatcaaa gaactgccgc tggttgatta
     7621 tatccttgtt ccgatcggag gaggcggact ggcaaccggt gtctccacac tggctaagat
     7681 gctcaacccg aacatccgcg tgatcggtgt ggaaccggca ggggcagcct gcatgaaagc
     7741 ttcccttcag gcaggagaag ttgtgacact gccaaatgtc aatacgatcg cagacggcac
     7801 ggctgttaag acacctggta aaaatgtgtt tccgtatctg cagaaaaacc tcgacgatat
     7861 catcaccatt gaggacgatg atctgatcgt tgccttcctt gatatggttg aaaaccacaa
     7921 gatgatcgta gaaaactccg gacttctgac cgtggcagcc ctgaaatatc tggatgtaaa
     7981 agacaaaaaa gtggtatcga tcttaagcgg cggaaacatg gatgtgatca ccatgtcttc
     8041 tgttgtacag ttaggtctga tccagagaga ccgtatcttt accgtatctg ttctgcttcc
     8101 ggacaaagca ggagagctgg ttcgtgtggc aaccgttatt gcaaatgaac agggaaatgt
     8161 catcaaactg gatcacaatc agtttgtaag cacgaaccgc aatgcagccg tagagctgaa
     8221 gatcacactg gaagcatttg gaacggatca caaagagcgc atcgtcaatg ccctcaaaga
     8281 gcatggctat aatccgaaag tgattcgccc gaatctttaa aaggagggga gcaatatgaa
     8341 aatagcagta gcgggaacgg gatatgtagg gctttccaat gccctgctgc tctctcagca
     8401 caatgatgtg tgggcagtcg atatcctgca aagtaagatt gatctgatca ataaagggaa
     8461 gtcaccgatc gtagacagag aaatcgaaga atttctggca agagaggatc tgcacctgac
     8521 agcaacgaca gatgcagaaa aagcttataa ggatgctgat tttgttatca ttgcaacccc
     8581 gacgaactac gatccgaaga aaaattattt tgacacatct tcgatcgaaa cggtgatcga
     8641 actgatacag aaggtgaatc cggataccgt gatggtgatt aaatcaacgg ttccggtcgg
     8701 ctatacaaaa ggaatccgtg aaaaatacca tacagaccat attctgttct caccggaatt
     8761 tttaagagaa ggacatgcac tgtatgataa tctgtatcct tcccgtatta ttgtcggatc
     8821 gccggagaat cagcgtgaca aggcacatgc atttgccgca cttcttcagg aaggggcgat
     8881 caagcaggag atcccgacac tctttaccga tcttacggaa gctgaggcag tcaaattgtt
     8941 tgcaaacacc taccttgcca tgcgtgttgc atttttcaat gaactggata cttatgcaga
     9001 ggtaagaggg cttgacagta aacagatcat tgaaggagta ggactggatc ctcgtatcgg
     9061 cacnnnnnnn nnnttggatt tttttcaata atgcgctctg caataaaatc ttttctggta
     9121 cggaagcaaa ccgtaccaga aaagatttta ttgcagagcg cattattgaa aaaaatccaa
     9181 agattgtagg cgtataccgc ctgacgatga aaaccaattc agacaacttc cgccagagct
     9241 cgatccaggg ggttatgaag cggatcaagg cgaaggggat cgaagtggtt gtatatgagc
     9301 cggcaatgaa agaagaaacg ttctttaact cccgagtgat ccgtgatctt aaggagttta
     9361 aagaagtaag cgatgtgatc cttgccaacc gctgcagtga tgagattgcg gatgtcatgg
     9421 acaaagtcta tacaagagat ctgtatttca gggactaagg aaaaatgagg agagataaat
     9481 aatgaaatta attatacagg tgccatgtta taacgaggca cagacgctgg aaattgcact
     9541 gaatcatctg ccaaaacata tcgacgggat cgatgagatc gagtatctga tcatcaacga
     9601 cggaagccgt gacgataccg tcaaggttgc gaaaaactgg ggcgtgaact atgtagtcaa
     9661 tttccggacg aaccgcggac tggcaaaggg gttcatggca gggctggatg cctgcctgcg
     9721 aaacggtgcg gatatcattg tcaatactga tgccgatgat cagtactgcg gagatgacat
     9781 cgaaaaactg gtgcgtccga tcctggagga aaaggcagat attgtgatcg gtgaacgccc
     9841 gatcgatgat actgagcatt tttcaccgct caagaaaaag cttcagcatt tcggaagctg
     9901 gaccgtgcgt gtggcatcca aatccgatat tcctgacgca ccgagcggtt tccgtgctta
     9961 tagccgtgac gcagcgatgc gtctgaatgt aacaaatgaa tatacctaca ccctggagac
    10021 gatcatccag gcaggacgca ccagaatggc aatggagtcg gtgccgatcc gtaccaatcc
    10081 ggagcttcgt aaatccagac tgttttccag tatgtttggt tatgtgaagc gttccatggt
    10141 gacaatcgtc cgttccttta tgatgtacaa accgctgcgg ttctttctcg ggatcggcag
    10201 tgtgctcttt ctttgcgggg cactgcttgg cgtgcgtttt ctggtttact atgcgaccgg
    10261 aaccggaaac ggacatgtgc agtccctgat ccttgcaagt atcctgatgc tgatgggctt
    10321 ccagaccggc gtgatcggac tgctggcaga catcattgcg gcaaatcgct agattttgga
    10381 agatgtacag tatcatgcac gtaaactgga ttatgacaga gagaaagagg aaaaataaaa
    10441 tgacaatgac gaaacaggac aggatttgta tgatctcact gtttgcggta atgctggtgc
    10501 ttatgggagt gccggcatta tgtctgagta cgaacacggc tgtcgatgaa atgggcacgc
    10561 tggcaaacgc agcattgctc tccggaagaa actggggaac cggagttctc agtaatggtg
    10621 gattttatta tcgatatgga atggcacttg tgtggtgtat tccatttttt ctgttaaaaa
    10681 aaggtgtgca gatttataaa gcagtttcgc tgatcaatgc actgcttatg tcggtaacac
    10741 cggtgatttc ctattatatt gccagacgat atctgaaaat tcaaaaagaa agatcagcag
    10801 cacttctggc agcagcttcg acgatgatca gcagtgtgat gttccaggcg atttacctga
    10861 gaggcgat
//
LOCUS       DAWDWH010000046        10713 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3197_length_10713_cov_9.208951, whole genome shotgun sequence.
ACCESSION   DAWDWH010000046 DAWDWH010000000
VERSION     DAWDWH010000046.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10713)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10713)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10713
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3197_length_10713_cov_9.208951"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1376..1385
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2048..2057
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3517 a         1751 c         2844 g         2581 t
ORIGIN      
        1 ataatgtcga agtagcactg gaaggcagtt tatgggcaga agatgacaca atttatatgg
       61 atgtagaaaa tatttacgga gaagcaaggg aagaagaaac ggaaaaggaa acacagaaaa
      121 aaacggaaaa agcaacggaa aagaaaaccg ataaaaagga aagcacttca gaagatctga
      181 cgaaaaatga ttatataatc cctgacagtg acaagaaata tctgacagat gcagatgtgg
      241 aaaatctgac attacgcgag atcaattatg cgaaaaacga gttatatgca agacatggaa
      301 gaaaatttga ttcaaatgaa ctgcgaactt attttgaaag taagtcatgg tacagaggaa
      361 cggtagatcc ggaagatttt tcggtaagct gttttaatca gtatgaaaat gcgaatgcag
      421 cttttctgaa tcagaaagaa aacgagcgtg gggtttatca gttagataaa taagaggaga
      481 aaacttatga aagataaaag gcgaatggta tggatcatca gcgttgtatg tatcattctg
      541 gctgttgctt tttgtgccgt cagtattgtg aaaatgaacc gtctccagaa acaggcaaga
      601 gatctggaaa ccgaagttgc cagattaatg tcagaacaaa atgaaaaaca gacagagaca
      661 ctgactgaaa cggaagaaat aaaagaagta aaaaaaacaa agaagaaaaa gtctaagaaa
      721 gcgaaaaaag aaacaaatac agaaaccgaa acagaggcaa ccgaaacggg gacagatacg
      781 gaaagtgatg tgcagacgga aaaaagtaca gaagcacaga atggacatgt ggttgccatt
      841 gatccgggac atcagggaag ctgggtgaat atgagcgaac aggagccaag tggtcctggt
      901 tcttcggaga tgaaagcaaa agcaacgaca ggaacgcagg gaagatatac aggggttccg
      961 gagtatcagc ttaatcttga catctcattg gcactgcaga aagagctgga aaatcgtggg
     1021 tacagagttg taatggcaag aacggacaac gacacggcga tcagcaattc agaaagagct
     1081 ctgaaggcat atgaagaagg gggagaaatt tatgtgagaa ttcatgcaaa tggaagcgat
     1141 gatagcagtg tgaatggagc acttggaatg acaccatctt atgagaatcc atatgtggca
     1201 aatctggcag aggaaagtta tcgactgaca gagtgtattt tgaatacgta ttgtaatgaa
     1261 acaggattta ataatcttgg aattcaatat cacgatgata tgaccggcat taactggagc
     1321 aaaatcccgg ttatgattct tgaaatggga tttatgacaa atgaacatga cgatannnnn
     1381 nnnnnactca gaaagtaaca gtgaacaagg aagtgattcg gaaacagcag cggagatgtg
     1441 gaatctggat ctggattccc tggtaagtga tgcagaagcg aacggtgaaa aatgggcagt
     1501 cagtgttcaa aatttgagtg atggttccgt ttgtgaaaaa aatgggaaag tttccatgca
     1561 gtcagccagt gtgatcaaag tgtttattat ggcaactgta tatgatcgaa tttgctatcc
     1621 gcagaataaa cagacagcga tttatatttc ggagcagtat gatggggaat tacgagatct
     1681 tttaagcaat atgataacgg tcagtgataa tgatgcagca aatcgtctgg ttgaattgct
     1741 gggaaacggt gattttaatg tcgggaaaga ggttgtcaat gcattttgca gtgcgaacgg
     1801 atacagtaat acgcatctcg gaaggcgatt tatggatacg aatccggcag atgacaatta
     1861 taccagctct tcggattgta gaaaaatttt gagtgatatc tataatggtg tatgtgtaaa
     1921 cagcgaagct tctgaaaaga tgctgcaatt tttaagtgct caaaccagaa aagggaaaat
     1981 cccatctggt attccatctg gaattcaaag tgccaataaa acaggagaga tgccggaagg
     2041 atatggannn nnnnnnntgg ctgtatcgaa aatgatattg ccattgtttt tggtgaacag
     2101 caggattacg tgatctgtat tttgtcaaat gatcttggcg gaaggaatga aagtgccatt
     2161 gagcggatta gaaatatttc atcatatgtg tatgaagttt tgaaatagta tcggcagtgt
     2221 ttatatacgt gaagatgaaa tgtatgaaag atacgagagg agagaagaag gagtgaaatg
     2281 cagaaaatgt ggaagtgaac tggattcgga ttctgttttt tgtccggaat gtgggacgag
     2341 agttgaaaga ctggtggaaa acaattccgg tgatattgtg agggataatg taaacagtgg
     2401 tacaacggct gatattccaa aacagacaaa aagaggaaat actttactca aaattgtgat
     2461 agccatattg atcgtaatta tattaaagag tgttgtgttt aaagaaaata agcagacggc
     2521 aggagaggat aaggattttc agtccaaaat ggcagagact gtgaaagaaa cgaaaaacac
     2581 agaaagtctc atagaaaata ttgcaaaaga aacagctaaa aatattgtga aggagacaga
     2641 aagaaaagca gaaagtgaaa tagaaatcga aacagaaagc gaatatgatg cagaattcta
     2701 taaagatgca ctggattcga tcaatgattc ggtatggctg caaggtactg tggcagagga
     2761 cggagaaacg ttaatgttag agaagagtgt caatatctgt gcagaagatg aaaaaaatga
     2821 gaagaagcgt aaagataata ttaaaagtgt aaagatattt tcagacgagc cggagatcgg
     2881 tgaggcaaaa gcagacccgg aaacaatgaa aggagcaaaa gtcagaatta atggagcggt
     2941 attctttatg ggcgattctg ttgtaatgta tgcgaatgat gctgtagtac tggaagaagc
     3001 tgtttcggaa gagcaaaagg tccatactta cattttgctt cagaaagatt gtacctggga
     3061 tgaagcattt gaagaatgta aaagaatggg tggttatctg gcaagaatta atacagaaga
     3121 agaatggaat cagattaaga agcaaatcaa agaagaagga aaagaaaaaa tccagttttt
     3181 ccttggtgca agaagaaaca gcggtgaaaa agaatactat tgggtagatg aggacaatga
     3241 atttacaggg gatcgactgg ataataatga tgagcagtgg tgtaaagatg tatggctgaa
     3301 aggtgagcca agctattatg attccagtgt caaaaaagat gaagatcgtc tgaatatttt
     3361 ctactattcg caggaagata aatgggtatt caatgatgta ccaaatgata ttttggatgc
     3421 agttccacag tttaaagaga gaatcggtta tatttgcgaa atagaagaat gagttttgag
     3481 gaaataagat gaaaaaaaag ataatgtacg cattgatgtt tacactgatc tgcatatgtg
     3541 caattgtgat tggaaaaata aagttttcgg gaacagaaaa acaacagcca tcttcgttgg
     3601 tttcagaaac agataattta aaggatagcg aatcaaattt tgttgaagaa gaaacggaaa
     3661 gaagtacgca aaagacagca gaagagcagg aaataatatt agaaaaggaa acaacatcag
     3721 aatcgccgca aacggagaca gcagaatcac agcaaacaga tttggtaaat cagatagact
     3781 atgcactgga aaaaatgaca gtagagcaaa aagtggcaca attatttatt attactcctg
     3841 atgcactgac aggtattacc ggtgtatcag cagcgggtga tgccacttat gctgcattac
     3901 agcagtatcc ggtaggaggt ctggtttatt ttgaaaataa tctgcagtca tatgagcaaa
     3961 tttcagaaat gctccataat gtacagcagt acagtatgga aatctcagga ttgccattgt
     4021 ttctggctgt agatgaagaa ggaggaacgg tagcacggat cagtgggcag ggatatggta
     4081 tagaaccgat tgaagatatg gcagcagtgg gagcatcagg agattacgaa agagctcagg
     4141 aaattggtac ttacatagga agttatctta gtgatctggg ctttaatgtt gactttgcac
     4201 catgtgcaga tgtgttgaca aatccggcaa ataccgtagt tgcacgcagg tcttttggca
     4261 gcgatgcagc tcttgtggct gaaatgacgg atttgcaaag aaaagcaatg gagcaggaag
     4321 gaattatagg ggcattaaag cattttccgg gacatggtgc gacaagtgag gattctcatc
     4381 agggatttgc ctatatccag aagacagagg aagaactggt gaataacgaa tggattcctt
     4441 ttaaaaaagg aattgaagaa ggggcgaaga tgattatggt cggccatatt gtttgttctg
     4501 aaataaccgg aacagaagat ccttcttcgt tgtcttatta tatggtaaca gaaattctga
     4561 gacagaaaat gggatttcag ggacttgttg ttacggatgc tctgaatatg ggagcgattg
     4621 ccaattatta tacgtctgcc caggcagctg tcaaagcagt tcaggcaggt gtggatctgc
     4681 ttttgatgcc gtctgatttt tcaggtgctt atcagggggt tctgaatgca gtttataacg
     4741 gagaaatttc agaggaacgt ttaaatgaat ccttgtatcg gattcttcag gtaaaaaatg
     4801 aaatgagagt gcacggaaaa tattaaataa aatgtgaatc tacttcaatt gcatatctga
     4861 gggttgcagg aaatgaatag aaactgcgat gaagcagagt aaagtgggat gtgtctagaa
     4921 agaaaaccgg acgtgttttg agtaccttat tcccgaaagc aaagttacac aatcataata
     4981 caatagatgc cttgaaccaa agggagataa atggtagtat atctacaaag tattttcctg
     5041 atgtttgggg catttttatt tgttttatcc tgagcaaacg ggaaaatcaa caaacaagac
     5101 tgggggacta caagttgata aagatggatc gtcagaagtt tatccggccg aatcgtcaga
     5161 tggttcggat atccagggag aagctgatca taccggagcg tgagtcagcc acacagcgga
     5221 tgctggctgg attgcagagg gaaacaaaaa cggcagatat agcaaagaca gaaaaaacag
     5281 caaatgaaaa tatagcagaa gctgtaaacg cagcaaacaa aaatatagaa aatgcagtaa
     5341 atacagcagc cacagaacat ctgcaaacga agcagcctga aatagcagag gcagtgaata
     5401 aaccagaaaa cagcatcgtc ttagcatgtg agatcgatga ggtgatcaac tatgcactgg
     5461 ttcacaatgg tgcttctgtc gtgcgtgata tctgcatcaa aaacacatcc gaaacggaac
     5521 ggaatgctct gcttttaaaa atcgcttcgg acggtgagct gatggaggat tttgtgtgcg
     5581 ggatcgaggc actgcagaca ggtgaggagc tgcattttcg aaatctggat ctgacattcc
     5641 atgtcggata tctggcatcg atcacggaga agttttccgg tcagctgacg gttacggttc
     5701 tggatgggga aactgtgctg gcatcggaaa agatcaatat cacggttatg gcgtttgatg
     5761 agttcccggg atttcagtat acgccggagc ttctgacatc ttttgcaatg ccgaatcatc
     5821 cggcggtggt cagtctgatc cagctggcat cgaaatatct ggagaaatgg acgggcgatc
     5881 cttctttgga cggttatcag tccggtgatc aggagcgtgt caaaaacatg gcggcagcag
     5941 cttatgcggc gatccagcag aaaaatatta catatgcatc gacagcgagc tttgaggcat
     6001 gcgggcagag agtccgcctg gcggatgcgg tgctggagca gcattttgga aactgcatgg
     6061 atctgacgct gctttatacg gcatgtctgg aagcgatgga tttaaatccg ttcatggtgg
     6121 ttgtagaagg tcatattttt gccggtgtct ggctggtgga cggagcgttt gcagatattc
     6181 tggtggatga tccgtcacag cttgagaaac gtatggcaaa aggcattcag gagcttacgg
     6241 tcgtggaatg tacggcgatg tgttccgggc agaatgtaag ttttgacgaa gcagcagcca
     6301 tggcaaaacg gcgtgtttcc aattatggga agttttattt tgcaatcgat gtgaagcgtg
     6361 ccagaagcag gggaatccgt ccacttccaa tccgggttca gacggcaaac gggtttgagg
     6421 ttctgcacga ggaccgaaaa gaaaaagagg tcacaggtgg atcagacagc aaaatcgaga
     6481 tctttgattt ttcggactgt acggaaaagg cacaggtcac aaaactgacg cagtgggagc
     6541 gcaaacttct ggatctgagc cttcggaata tgctgatcaa tatgcgtgtc acaaagagtg
     6601 ttgtgccggt tttgtccgcg gatatcggaa gtcttgagga tgcactgttt gacggggaag
     6661 cgtttcaggt gatgccgcgg ccggctgatg taggtgtgaa tccggcagca gaaagtcaga
     6721 tcgaacttct caacaaactg ggagaattat cagaatttat ttcttttgag agcaaacata
     6781 aaagactgca ttccatttac agcgagaaag atttaagcac ttctctgacg aaaatgtacc
     6841 gttctgcaaa agcatctctg gaagaaaatg gagcgagcac gttatatctg gcacttggta
     6901 tgcttcgttg gtttgaacag aaaaagaacg cacaggcaag atacgcgccg atcgttctgg
     6961 taccgatcga cattgtgcga aagtctgcgg gcagaggcta tgcgatgcag atgcgggatg
     7021 aggattcaca gatcaacatt acgcttctgg agtttctgaa gcagaattat gagatcacga
     7081 ttcctggcat gaatccgccg ccgcaggatg agcatggaat ggatatgccg aagatttttg
     7141 caatgatccg aaaagcggtg atgagcatgg aaatgtggga tgtgctggaa gttgccgtga
     7201 tcggaaactt ttctttttcg cagtttgtga tgtggaatga tattcataac aaccgtgatt
     7261 tcctggaggg aaacaagatc gtccacagtc tgatcgaagg agctgtggac tgggactgca
     7321 cgatcccgga agaagtggat caggaggagg cttatttacc ggtaacggcg gatgcttctc
     7381 agctgcatgc catcaatatg gctgccgcag gggtgagttt tgtactgcac ggaccgccgg
     7441 gaaccggtaa atcgcagacc attacagcat tgattgcaaa tgcactgaca aaaggaaaga
     7501 cagttctttt tgtggcagaa aaaagagctg cactggaagt ggtacagaaa cgtctggcgg
     7561 cacttggaat cgatgatttc tgcctgaaat tacattcgaa caaagctacg aaaaaggcag
     7621 ttttaaatca gctgcggcgt ggtcttgaga ttgacatgga aggcacaaaa acagattatg
     7681 agcagagaat tgcggacatc catgcgatgc gtaccgaact ggatgcttac gcaaaagcac
     7741 tacataaaaa aagaaatttc ggaaagagtc tgcgtcagct gatcgatctt tacgaaagta
     7801 ttccggaaac aaaagaacag gtaaaatttc cggtatcgga tatcaaaaat ataacagaag
     7861 gtgatctgag cagtcagaaa aaccatctgg aacgtctgat ctcagccgga agatccatcg
     7921 gtcatccgta tcagcatccg cttgcggcag tgaaaagggc acagtacagt cagcatctga
     7981 aaacggaact ggagccggcg atgcaaaact atctgtctgt acttgataac atccaggctc
     8041 agatggcgga ttttgccgcc tcttccggaa tcaaaaaccc ggtcacaaag gcagaatgga
     8101 atcaggtatc cgaactgatc aggtgtatgg aaagtacgga aaaaatgcct gcctttttga
     8161 ggacaacaga ttctgttgaa cgtgaatttt ccgttccgga agaatatctg gaaaaacagg
     8221 agaaatttga acagaaaaag gcagaaatgc tttcaaaatg gaacgaaaat ttcttgagtc
     8281 aggacatgag ttcttatcat gtgaagtacg aagaagcaaa caaaaaggta tttttcttaa
     8341 aaggaaaagc acaggcggca cttgtggcag aaatgcagct gtttgccgta tttccggtgg
     8401 aggtcgagca gattccgctg tatctgacgg agatcactgc ttatcagcag gaagagcaga
     8461 aaatggaagc ggcagaagca gaactgtctt atgactggaa acagatcttg aaagaacatc
     8521 tgacaaatga agaattgcag tcttataaag cacagatcca aaatcagaga aagagtatgg
     8581 cacgttttgc cggagtgctt caggaaatcg aggcgatggg cattttaagt acacttgtcc
     8641 gaaagggaaa agcactcttt tctatctgga atagcatgga tgctgcagag cgaaaggttg
     8701 cagagttgct ggaactgaaa ttttgcgatg agaaagcaga ctggattgag aacagaaagg
     8761 cactttgtag ctgtattctg gaaaatgcag ccgcattaaa agactggatt gtttatcagc
     8821 agaatgtaga tttgtgccgg aaaaacgggc ttgctccgat ctgtgatgcc tatgaaaaag
     8881 gccttccgca tgaagtggta ttgaatgtat atctgcgttc catttaccgg gcgatcattc
     8941 ttggcgtgat cgaatccgaa ccggtgctga acggatttac cggaaccggt tttcaggttc
     9001 agatcgggca gtttaaaaag ctggatcagg aatgtatgca gctgacgaaa gaagaaatgt
     9061 tttaccggct gtcatcgcag cttctgtcgg tcggtgattc ggtggaagtc agcagagaac
     9121 tgaattattt aagaagggcg atcagcagca acggcagagg tatttccatc cgtctactgt
     9181 ttgaacagat cccgcatatt ttgcagagac tctgtccgtg tatgctgatg agtccgattt
     9241 cggcggctca gtatttaaag gcagaaaatg atctgtttga tattgtggta tttgacgaag
     9301 catcgcagct tccgacctgt aaagcagtcg gtacccttgc aagaggcaaa aatgcagtga
     9361 ttgtagggga tccgaatcag atgcctccga ccagcttttt tgcgggaaat tcggtggatg
     9421 aggacaatct ggatatcgaa gatctggaca gtattctcga tgactgtctg gcacttggca
     9481 tgccgtccgc acatctgcac tggcattacc gaagccgcca tgagagcctg atcgcttttt
     9541 gtaaccggga attttatgaa aattctatgc ttacgttccc atcggtaaat gaccggcaaa
     9601 gacgcgtcag catggtaaaa gtggaaggct tttttgatcg cggcaagagc cgtgtgaacg
     9661 aaggagaagc acaggcgatc gttgcggaga tcaagaaaag atatgccgat ccagagcaga
     9721 aaaagcagac gatcggtgtg gttactttta atgttaatca gcagactctg attgaggatc
     9781 tgcttcagga agaatatcag aaagatttaa attttgataa atgggcaaat atgggagaag
     9841 agccgctgtt tgtcaaaaat ttggagaatg tccagggaga tgagcgtgat gtgatcctgt
     9901 tttccgtggc attcggaccg gatgcggaag gaaagctgtc catgaatttc ggaccgctga
     9961 accgggatgg cggatggaaa cgtctgaatg ttgcggtgtc ccgtgcccgg tgtgaaatgg
    10021 tggtgttttc tgttatgaca gccgatcaga tcaatttgag acgaacaaaa gcaaaaggcg
    10081 tagaatctct gaaatatttc atggaatttg ctcaaaacgg aaagttgcgg ggagactaca
    10141 gacagaatga agcagtcaaa aatcagggga tcaaagcaaa aatctgccgg gcacttgcag
    10201 atgctggata cgagtatcag ttaaatgtcg gccattcaaa attcaaagtg gatatcgcag
    10261 tgattaatcc ggatcatccg gaagaatatc tgctcggaat tatgacggac ggagattctt
    10321 actgtcagtc gtcaaatacg aaagaccgtg aggtggcaca gtttgaagtg ctgaaaggac
    10381 ttggatggaa actgtatcgc atctggacga tggactggtg ggatgatccg caaaaggaga
    10441 ttacaaaact tctcggagtg cttgaaatga gaaagaagga agaacagtaa cagaaaggaa
    10501 gcaaaaatgg gaaaagaaaa ggaaaacaga aatggaaaaa caaaaggaaa tgcgatcgct
    10561 ctgttgccga tcggcgtatt tcttgtgctg tatctcggac ttggtatttt atttgaatac
    10621 gtgatgaaaa ttccgatggg attttataat gtgccgattg tggttgcttt tctggcagcg
    10681 atcctggttg cctgtctgca gaaccgggcg gta
//
LOCUS       DAWDWH010000047        10626 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3224_length_10626_cov_12.168953, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000047 DAWDWH010000000
VERSION     DAWDWH010000047.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10626)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10626)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10626
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3224_length_10626_cov_12.168953"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6390..6399
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8900..8909
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2750 a         2502 c         2043 g         3311 t
ORIGIN      
        1 ttttttcaca gcttacttct tttcccttga atttatccag cacaaacttt tccggatcat
       61 ctgtgcagat ctcatcgata aagacatccc gcatctgatt attcgcacat tcattaaaaa
      121 tttcccagac tgtttcatat tctttcattc tgattccctc tttttctatt tgatcgtaat
      181 atttaaatcg aacgcatgtg agacctgaca cgcgattccg gtcagctttg ctgacattta
      241 cctgacagaa ttgctgtgca gtctgcactc catagcatct gccaaaaaag gattctgatt
      301 tttttgctca cagatggcat ccagaataca gtctgctgcc tgtgccttgc tcatcaggtc
      361 aaatgcctgc tcactgtctt ttgtgatgat cgtgatcacg ttggtatctg tgccaaatcc
      421 ggctcctgct gtttttaaat tatttgcaac gatcatatcc agatttttct tttccagttt
      481 tttgcgggaa ttctctataa cgtgttccgt ttccatggaa aatccacata aaaatgtttt
      541 tgcggttctg ttttccccaa gatattttaa aatgtcctgt gtgcgggtaa gggcaatgct
      601 catctcgcca tcagattttt tgactttctg atcataaact tcactcggac gataatctgc
      661 tactgctgca gccataatca caatatctgc atccgcagcc tcttctttga ctgcttcaaa
      721 catctgttgt gcacttttta ccggaaccat ctttacaaaa tgcggctcct ctgcctcggt
      781 gtgccctttt acgaccgtca cggaagctcc acgcagcatc gcagctttcg ccagggcaaa
      841 tcccattttt cctgttgaat gatttgtgat atagcgaacc ggatcgatcg cttccatcgt
      901 agctccggca ctgaccagaa ctttcatccc tttcatatcc ttttcacgtg caatgcttct
      961 cacaatatgt tcaaccagaa gcttttcttc cggaagtttg ccgattccgg aatcaccaca
     1021 ggcaagatgt ccgctcgccg cctccaccat ctcaaaacca tagttctgaa gacgtttcat
     1081 attgtcctgc acgatcggat tgaggtacat attggtgttc atcgccggtg caatgatctt
     1141 ctgacaagtg catgcaagtg ccgtcgttgt cagcatgtca tctgcgatcc cgtttgcaag
     1201 ctttcctatt atattggcac ttgccggtgc gaccatgaga agatctgctc tctttgccag
     1261 tgaaatgtgc tctacctgaa actgaaaatt ccggtcaaac gtatcaatca gacatttatt
     1321 tccggtgagc gtctcaaaag tgatcggatt gataaaatta actgcattct ttgtcatgat
     1381 cacttccaca ttacaatgca gtttctttaa catacttgcc acatttgcca ttttatatgc
     1441 tgcaatactt cctgttatcc caagtattac cgtttttccc tttaacatca caaaacctca
     1501 cgttcttttc taaattattt atgcaatgat aaaacttttt tgtttatcat atcatacttt
     1561 ttatgtattg tcagcctgcc tggaaaaatt acactgtttc ccttgtcatt taaaatctgc
     1621 tatgctatga tactgatgtt tatgagccaa ggagcaaatg gacacaaaac agttcctgtt
     1681 tttcctttgc ccggctactt atatccatat ttttcaggag gatttttact atgaaaaaag
     1741 acactcgata cctggtcagt gttgcactta tggcagcgat cgtcattctt ctggctaaca
     1801 caccgcttgg aatgatccag cttccgatta ttaaggcgac aactgtacac atcccggtta
     1861 tcatcggtgc gattcttttg ggtccatccg ccggtgcaat cctgggaacg attttcggca
     1921 tctgttccct gatcagcaat acaaccgctc caacacttct ttcgtttgct ttttctccgt
     1981 ttctgagtac aaccggtctt gttggcgtgg taaaagcgat ctggatctcg gttggatgcc
     2041 gcattatgat cggcgtgatc tccggatggc tctggatctt atttcgaaaa ctgaaagcga
     2101 attcttatct ttcccttatc atcactggtt ttgtcggttc gatggtaaat acgatatttg
     2161 ttatgggcag tatttatctg ctctttgccg gacagtatgc cgctgccaaa gatgtggcac
     2221 gcaccgcggt gttcggcctg atcatgggta cggtcactgc atccggcatc ccggaagcga
     2281 ttgctgccgc aatccttgtc actgtcatca caaaagcact gctgaaggtt gttcctaatc
     2341 tttcaaacgc ttctgctaac taaatcgcag gcacagcttc cgtgcgttcg atctgaatac
     2401 atacgctctg tgcctttgca gaatggagga tattttgatt cttgcaattg atatcggaaa
     2461 tacaaacatt gtggtcggct gtatccgtgg ggacgaagtt ctcttcaccg aacgcctttc
     2521 caccaataca acacatacaa ctctggaata tgctatcagc tttaaaaatg ttctggaaat
     2581 ttatcagatc gatgcaaagg aaatcgaagg tgccatcatt tcttctgttg tcccgccgat
     2641 caccaatgtg atccgtgatt cgatccataa aattaccgat gctgaagtgc tgatcgtcgg
     2701 tcccggtgta aaaaccggtc tgaatattct gatggataat ccgaaaacac tcggaagtga
     2761 cctggttgtc aatgcggtgg ccggcatcag tgaatatccg gttccgctga tcctgatcga
     2821 ccttggcaca gcgaccacga tctctgtgat cgacaataag aaaaattata tcggcggcat
     2881 gattcttccg ggaatccgtg tttctaccga ctctctgacc agccgcactt cacagctacc
     2941 gcgtatcggt gtggaagaac cccgccgcct gatcggaacc aacaccatcg actgcatgaa
     3001 gagcggtatg atctacggaa atgcttcctg tctggatgga atgatccagc gtatcaaccg
     3061 tgaacttggt gaaaaagcaa cggtagtcgc taccggaggg cttgcacggt tcatcgttcc
     3121 gcactgtctg gaacatgtca ttcttgatga cgagctgctc ttaaaaggtc tgaaatacat
     3181 ttattataag aatcgcactc cacgtcatgc cggataaaag tacttttaga acaaatttct
     3241 atataccgag actgaaatct gcaccgttct gaattgttat acaaaaatat ttactgttct
     3301 ttcaaaaaag tcattgacaa cttcagaaat atttgctaat atataggacg tgtcacggac
     3361 acaatacagg ggattggtca aatggtatga cagaagtctc caaaactttt agcgggagtt
     3421 cgattctctc atcccctgtt atgaaaaccg aaaggtctca aaagactttt cggttttttt
     3481 gcatattctt aactttattt tttgtctgat tgtcggtctg tgcattctta tgagcaaata
     3541 tgcaatggat atcacaagac agaagtacac aaaaaaacga cctcacttcc atgatcgaga
     3601 tctggaatga ggtcgttgaa gatggcgtta tttccaacat ccatgcacgt catctctgtg
     3661 aacgtcttgg atttgtccag cctggtgtcc tggtgtcagc ccttttcttc ggattcccgc
     3721 aaaaacagaa ccggaaatat tgtgccagac actgaagatt gctcccggaa gtgttgccag
     3781 cgggcttgtc gcaaaatttg cagtcgcaag ggaaacggca agtccgctgt tctgcattcc
     3841 gacttcaatt gcgattgctg ttgccttgga atattcaata tgaaatattt ttgctgccag
     3901 aaggccaagt cccataccgc acagattgtg gatcgccaca acaatcagta caagcacacc
     3961 gcagcttaca attttttctg cattgatggc cacaattccg gaaatgatca tcacgatcga
     4021 tacaacggag atcagcggca gcacttctga aattttttca atctgctttc caaagagtgt
     4081 ccggatcaaa attccaagaa gtaccgggat caggattact tttaccactg agatcaccat
     4141 tgcccagaaa gacacttcca cccaggttcc tgccagaata taaaccagaa gcggtgtcat
     4201 cagcggagcg atcaaagtgg aagcgatcgt cataccaaca gaaagtgcca catcgccccc
     4261 tgcaatatac gtgatcacat tgctcgccgt tccgccggga cagcagccga ccaggatcac
     4321 accaagtgcc agttccgtcg gaagttttaa aatcatacag agggtccagg caatcagcgg
     4381 catgatcgta tactgcgaca caaatccgat cactacttct tttggtcttg aaaatacaac
     4441 cttgaagtct tccatccgga tcgtgagccc cattccgaac atcgccactc caagaaatac
     4501 cgaagtgtat ttagtcgtcc acgcaaaacc gtcccgccag aaaaaagcaa gcacggaaaa
     4561 tgcaatgatg atcacaccga tgtatttggt caaaaaggaa ctgatttttc cgattttatg
     4621 catgatattt ttcataggct acacaccata gctgaagttg tcgatcagtc ttgtttttcc
     4681 gatatagact gccatcgcta ccagcacatc acggtctgct ttttctatcg gctgcatcgt
     4741 aagtgcatct accatcataa cataatcaat acgtgccagc ggttctgcct caatgatcgc
     4801 tttcatcgga ttcagcactt cttttgcatc tgctcctgtg taaattactt cttttccggt
     4861 ctttactgct cttgaaaggc aaagtgccgc ttgtctctct ttatcattta agtaagtgtt
     4921 tctggatgat tttgccagtc cgtcttcttc acggacgatc ggacatccga caatctcaat
     4981 atcaaaattc agatctttta ccattttgcg gatgattgca agctgctgtg catctttttc
     5041 tccaaaataa gcacgatccg gtgctacaat gtggaacagt tttgtgacaa ccgtgcacac
     5101 acctttaaaa tgaatcggtc ttgtttttcc gcaaagtgtt tctgataagg tatcaatact
     5161 tacaaaagca tgtggatcat gatacatatc ttccggagac ggtgcaaaga tcagatctgc
     5221 tccaaggctt tcgcaaagct tactgtctct ttcgaaatct ctcggatatg cctctaaatc
     5281 ttcatttggg ccgaactgcg tcggatttac aaaatcagaa accacaacaa tatcgttctg
     5341 ttctctgcat ttacggatca gacttgcatg cccttcatga agaaatccca tagtcggcac
     5401 caggccgatg gtctttcctt cttttttcca ttgttttatc tgctttcttg tctcctcgac
     5461 tgtttttgtt acctgcattt tgtttctctc ctctttttat agattacgag atagtctctg
     5521 aactgaactt caaaaatcat gctatatcat atacaaaaca gaatcctgtc acgaatacag
     5581 gtctggttca gatcaatact gatatcaccc caactgtcca tgtttttcgt aacatgaaat
     5641 tctgctgatc ttgtttttct catctacgaa cacaacggat ggtttatggt tcttcgcctc
     5701 ttccggatca tactgtccat aagccatgat aataatttta tctccagtgc tgacacagcg
     5761 tgctgctgca ccatttaaac agatcatgcc acttccgcgt tccccgcaga ttgtatacgt
     5821 ttcaaaccgg cttccattgt tcacatccac gatctgcact ttttcatatt ctaaaattcc
     5881 tgctgcttcc agaagttctt cgtccacggt aatacttccc acataatcca gctccgcctg
     5941 aataacggtc gcccggtgaa ttttaccttt taacatttct actgtcattt ctaattcctt
     6001 tccatgctca cagaaaaatc aagtcagttg actctttcaa tccaactaaa aaagcacctt
     6061 caaggcataa agtgaccctg aaaatgctgc ttcatttccc tgttttcaaa gtcttacttt
     6121 aatcgtttta aatataagct ctgttttttg attttccctg tccaggttct gagaattttc
     6181 tctgtccccg ggatctgcat tgtaatgttt gatttttttc attcagtacg agtatagctt
     6241 tgcctgcaaa tactatctca tgcggtatta tggcattgtt ttccaccctt gtcaatgctt
     6301 atctcagtcg agaagactcc cacttctgat ataattttct gttgtcgaga aacaattatc
     6361 acttttcttt tgcagataag tgcggtatgn nnnnnnnnng aatatttctc aattttaaga
     6421 atcatatata attacgaatt ttgaacgtag agaagcggat tgtgaatgtc cgctttgcca
     6481 attcaaggag ggcatatgac acttagagaa ctaaagatag gcgaaagtgc tgttgtcaca
     6541 cagcttggcg gcgaaggtgc tcttcgtcag cactttcttg atatgggcat gatccccggt
     6601 gcagaggtca cggtccagaa aatggctccg atgggtgatc cgatggagct gaaaatccat
     6661 ggctatgaac tgacactgcg tcttgcagat gccgagaaga tccaggttga aaaaatttct
     6721 gagaaaagag aacatccact tccgaaaaag aaacatttca agcgggaaca tccggggctt
     6781 ggcgagggcg gcaaatatca tgaaaaaagc agtgagcatc cgcttccgga cggcacggta
     6841 ctgacttatg ctctggtcgg aaaccagaac tgtggaaaaa caacactctt taatcagctg
     6901 accggctcca accagcacgt cggcaatttt ccgggcgtaa cggttgaccg gaaagacggt
     6961 gcgatcaaag ggcatccgga cacactggtc acagatcttc cgggcattta ttccatgtct
     7021 ccatacagca gtgaagaaat tgtttcacgg aattttgttt taaatgaaat gccaaaggcg
     7081 atcattaata tcgtggatgc caccaatatt gaacgaaatc tgtatctgac catgcagcta
     7141 cttgaaatga acatcccgat ggttgtcgct ttgaatatga tggatgaagt gaccggaaat
     7201 tccggttcaa tcgatatcaa tgacatggag gctttacttg gtgtaccggt catcccgatc
     7261 tctgctgcaa aaaatgaagg tgtggatgag cttgtccgcc atgcccttca tattgcaaaa
     7321 tatcaggaac gcccgaccag acaggatttc tgtgatgaga atgactatga tggtgccgtt
     7381 catcgctgca tccacgctgt gatccatctg attgaagatc acgccaggcg tgcaaaactt
     7441 ccggtacgtt ttgcagcaag caaagccatc gaaggagact ctctgatcct ggagcagctt
     7501 gcccttgacc agaacgaaaa agaaatgctg gaacatatcg tgctgcagtt ggaaaaagaa
     7561 cgcggtcttg accgcaatgc tgccattgct gatatgcgtt tctctttcat tgaaaaagta
     7621 tgccgtcaaa ccgttgttaa gccaaaagaa agcaaggaac atatccgcag ccagaagatt
     7681 gaccgtatcc ttactggaaa atatactgcc atcccatgtt ttgtcggaat catggtcgct
     7741 gttttttacc tgactttcaa cgtgatcggt gcatgtcttc aggatcttct tgcactcggc
     7801 atcgatgcac tgacaaaagt aaccgatcat gtgctgactg ccgcaaatgt caatccggcc
     7861 attcacggac ttgtgattga cggaatcttt tccggcgtcg gctctgtttt aagtttcctg
     7921 ccggttatcg taaccctgtt cttctttctt tctttaatgg aagacagcgg atacattgcc
     7981 cgcgttgctt tctttatgga caaactgctc agaaaaatcg gtctgtccgg cagaagcatt
     8041 gtaccgatgc tgattggatt tggctgtacc gttccggctg tcatgtccac cagaacactt
     8101 ccaagtgaac gtgaccggaa aatgactatt cttttgactc cgtttatgag ctgcagtgcc
     8161 aaacttccaa tctatgcatt ttttgtaaat gcattcttcc cgggacgtgg cgggctgatc
     8221 atggcaggac tttatattct tggcattgtg atgggtatcc tgatcgccct gttttataaa
     8281 ggcactttat ttaaaggcga agctgttccg tttgtcatgg aacttccgaa ttatcgactt
     8341 ccgggcttta aaaatgtaag tcagctttta tgggaaaaag ccaaagactt tctgcagaga
     8401 gcattctccg taatcctggt cgctactgtc gttgtctggt tcctgcagag ctttgatctg
     8461 catctgaaca tggtaacgga ttcacagaac agcatccttg ccacaattgc gggaatgatc
     8521 gcaccgctgt ttcaacctct cggacttggc gactggcgga tctgtacctc actgatcagc
     8581 ggatttatgg caaaggaaag tgttgtatcc acactggaaa ttctttttgc aggaaatgta
     8641 aacactgcac tttccaccct gtcggccgca tcacttcttg tattcagcct gctctacacc
     8701 ccttgcgtgg ctgcaattgc atcaatcaaa cgtgaacttg gacaaagatg ggcgatcgga
     8761 gtggttgtat ggcagtgtgt aatcgcgtgg cttgcagcat ttgtggtaca tctgattgga
     8821 agttttctgt gattctgtaa aaatgcacaa aagacagaga aaacatatta tctacgtttt
     8881 ctctgtcttt tatcttagtn nnnnnnnnnt cggttaaacg tttttctata tctgatcact
     8941 cttccagtgc ttttaatatc tgctcattca tcagatcata agaagcccag ttttcttttc
     9001 ttgtattgat tttcacacat tcacaaatgt attttataat ccacatggtt tcatctactg
     9061 tttttccggt tctctctttt acccattcct gtgtaacaga aacaatattg tgagctgcaa
     9121 tatttcttag tttatgctct acttctacca gttcatttac tctttgtgca aggagtttat
     9181 cttcacattt tgcctgaatg atattattta atgcccccga ataaaccgga ccatagcgga
     9241 aataagaata atttccctgt gcaagcctta aaatttctgt tccactcatt ttctgctcat
     9301 cccattctcg aaaagctttt cgtttgtaat attttgtaat gtcaatatca caaaactctg
     9361 ctaaaaccat ttccagcagg tccacgctaa gaggtgtaat cgcccttaaa aaatctgcat
     9421 attctccacg ttttactttc agatcaagaa taagcgtata ctcaaaaagc actttctttt
     9481 tatcttcttc atgaacagct tttaatattc catttgcttc cggcagaaca ctgttcctgt
     9541 cgttcttcgt gctcaatatt gctttttttc tgtggcgtag aaacctgaat tggtaaaaat
     9601 cgatattctg ccagtgttgc cataataaga agtgcacttt tcatggcagg cgttccggat
     9661 gccatattaa ccagaagtct gtcatcttcc cctttttctc tttcgatatc gctgatgatg
     9721 cttctgaact ctttgtaaaa tacatcatat tgctgcacat tcaccatctg atcattttct
     9781 atgatatgta cctcaaatat gtggtttaat ttttttccca acaattcaat acttctgaca
     9841 taacgattat ctgtcctatg atattccatc atttctttcg ataaatacaa ataaacaaca
     9901 tccggcttat aataccggca aatatgtaac atagatccat cataaaaata cttcacagga
     9961 tcggtgtttc caatcggtga aaataaataa gttcggctca tttttcttcc tttcgtgttt
    10021 ttatatcaaa ataacatcct cttccggttc cggtgtatcg ccccaggaga tcactttacc
    10081 ttttccggta atgcggtaaa ttctcacgct gtcttctgta cttcttacac taacatattt
    10141 cggaatttct tccattaatt tattgtaact tttggatgaa agcatcgcct caaaagcaga
    10201 tttctgcacc cgttttccat aaccttccag catttttgaa aatttgattc gtttcttatt
    10261 gtcaacgata tcgtatatga tcagtacaaa ttgtttatcg tcttctaatg cttcgtttat
    10321 ctgaaaataa taatcttcat catcgaataa taaccggctc atacaaatct gcgtcccctt
    10381 cctcaatcgc ctttgtcagg cgttccatct gcagtaaaat tgcccttcga aaactgactg
    10441 cataatctgt gtatttcaaa tagcggattt ctgtctgaaa ttttcgttcc agtttgttca
    10501 gataaatttt cagtccgtct ctggtaatat aacatccggg ttcatccata tcaaaataaa
    10561 actgatcttt tgatatttca tgtccattga tcatgctcat gactgttgca tccacgatca
    10621 ctgcac
//
LOCUS       DAWDWH010000048        10533 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3254_length_10533_cov_14.043902, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000048 DAWDWH010000000
VERSION     DAWDWH010000048.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10533)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10533)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10533
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3254_length_10533_cov_14.043902"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2788..2887
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8767..8776
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9448..9458
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2274 a         2564 c         2134 g         3440 t
ORIGIN      
        1 cgcctttttt cagcagttct tcatgtgttc cgctctctgc gatcccatta tcggcaacaa
       61 caagaatctg atctgccccg cggatcgtgg aaaggcggtg tgcgatcgtg attgtggtgc
      121 ggttctttgc cagttcttcg agacttttct gaatccatcg ttcgctctca ttatccagtg
      181 cactcgttgc ctcatctaaa ataaggatcg gtggattttt caggaaaact ctggcgatgg
      241 aaattctctg tttctgccct cctgagaggc gtgttccgcg ttcaccaacg tagctgtcgt
      301 atccatccgg cagtgacatg ataaaatcgt gaatatttgc ttttttcgct gcatcgacga
      361 tctcttccat ggaggcctct ggttttccgt aagcgatatt atcccggatc gttccgcaga
      421 acagatagac atcctgctgc accagtccga tctgatttcg cagactttca agtgtcagtc
      481 ctctgacatc ttttccgtca atcgtgattt ttccttccgt cacatcgtaa aaacgtggaa
      541 gcaacgaaca gatcgtggtc ttaccacttc cggacggtcc gaccagtgcg atcgatctgc
      601 ctgccggaat ccgaaagctg acatgagaca gtaccggtgt ctcatcatcc tgataatgaa
      661 aggaaacatt ttcataagat acatcaccac atacattggt cagtggtttt gcatcttcgg
      721 catcctgaat atccggttct gtctcgatca catcgagaaa tctgcgaaat ccggacaggc
      781 ctttctgcat catttctgtc agctcaacca gaatctggat tgggctgatg aagattccga
      841 tgtacagagc atacatggca agatctgctg cttccatttt tccgtgtgcg atcagccatc
      901 cgccaaagac cagggtcacc agatacatca gtccctggaa aaacagattg ctgctcataa
      961 aacttcccat acaacgatag ttcgcatctt tggataccag aaatccttcg ttgctcttct
     1021 gaaatttctt ccgctcaatc tcctcatttg caaaagactg tacgacacga atcccggaaa
     1081 gggtatcctg aagtctcgca ttgatatctc cgatcttgcg tcggttatcc atgaacgttg
     1141 cctgcatacg gcgattctga cacagggaaa agatcagcat cagcacaacc agaaacagaa
     1201 gcggcactgc cagtctccag ttgataaaaa ataagaagat aaaggctccg acaatcttaa
     1261 tcagtgaaat gaacagattt tccggtccgt gatgtgccag ctcagaaatg tcaaacagat
     1321 ctgatacgag tttgctcatc atctgaccgg aattatgctg attataataa gagaacgaca
     1381 gtttttcata atgctcaaac aattcctggc gcatatcccg ctccatcctt gctcccatca
     1441 tatgcccctg acacgtcaca taatacttgc acaacgcctg tattgcatac ataagcagca
     1501 atcctgcccc gataggaagc agtgcctgta agatcacagc tgcatctttt aaaaacagtg
     1561 tttttgtcag actgcgaagg atctgcggaa atgccagatc cacaaaactg ataatcgctg
     1621 cacacagcaa atccagaaaa aacacacctt tgtaaggtgc ataatatttc acaaactttt
     1681 tcagtgtctc cacttttgtt accttctttc gttattataa ttgaaccaaa tcaaggaagt
     1741 cccctgcttc aattgcgaat atccgcttga cacgtaagac ttggcacgcg attctgttca
     1801 gctttgctga catttaccca acaaaatcgc cgcatgccta ttgtagcatg cgacgattct
     1861 cgttacaact tgaacttctg ttattcaaga gattctttta tttactttac tttcgctact
     1921 ttattataag ttgccttttt gctgattttt gctttctgaa ggttcttttt cagtgctctc
     1981 agcgttttgg atttcatctt cttaggaact gtaactttca tcttagaaga agtacccttg
     2041 aatgcattcc tgccaacttt cacgatcttt gttcccttga aagttacgct tgacagcttg
     2101 ctcgctttat tgaatgcatt ggagccgatg ctcttgacgt tgtttccaat cacgatgctc
     2161 ttcagtttct tgttgttctt gaaagcattc ttaccgatcg tggttacttt gaagctgtaa
     2221 tttccaactt ttactgtatc ctggatcgta acttttgttt tcttcgttgc tttcagtttc
     2281 actgcagaaa ccgttccgtt tactgcatca gctttcgtta ctttgtaagt aacgcctttt
     2341 actgtaatgg ttgttccaac tgccggaacc tgtggtgccg gattattgtt tccgttgttg
     2401 ttattgttat catctttctt gacaagagct gcttttgcat cgctgactgc tttcagtgct
     2461 gcatttactt ctgcctcagt agcatcctta ttctccagaa ctttcttagc ttctgccagt
     2521 gctgctttca gtttgcctgc actctcagct gtgtaatctg cttctttgaa tgattctgct
     2581 tctttgactg ccttgttcag ctcttcgact ttttctgccg gtgcaggggt cggagtcggt
     2641 atcggatcaa tatcttttga ttttaccgtg atggtaactt tgcctgttac accgcttccg
     2701 tcttttgcag ttgcaatgat atctgcactt ccaacaccaa ttgctttgat cagaccactt
     2761 tcgtttactg ttactgcact ttcatttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnngag cttgtccatg taacttcttt gttgtctgca ttttccggta atacagctgc
     2941 agctgcctga gcagtaccgc cgatctccag ttctttctta tctgctgtta cgctgatgct
     3001 tgctactttg actggctccg gatctggagt aacctgtttt gcttttaccg tgatcacaag
     3061 ttctcctgat actccgcttc catcttttgc tgttgctttg atggttgctg ttccggctgc
     3121 ttttgctgtg atcagacctg cttcgcttac gtttgccact gtttcatctg agcttgtcca
     3181 tgtaacttct ttgttgtctg cattttccgg taatacagct gcagatgcct gcatcgtatc
     3241 accgacggtc agctctgtct tatcagctgt tacgctgatg cttgctactt tgatcggctt
     3301 cggatctgga gtttcactct ggaacgtgaa ttttgcatct gcccacagtg cgtggtcgtt
     3361 gtaatctgca tcgccttctt ctacgatcag acgcagatct tttactttgg atacatcgat
     3421 atctacgagg taggactgtc cgttggagat cacatggtct ttgcggtaca gctctttgcc
     3481 atcacctttg atcacatagt ttacatctgc tttgtttcca agtgtctggt ctgcactgat
     3541 accgatgcgt gcggtaaatc tgcttacgcc cttgccttcc aggttaacgc ccagttctgc
     3601 cggtgcatgc atagccacac ctttgtcaaa gctctgtttt gtgccttcta catagactgc
     3661 catcggattt ccgcatccgc aggcatctct tgtgttttcg aaatatccgc cctggtcaac
     3721 tgtccagtcc atatcggata catacagttc atcctgaact ttgaagttga tcgtataagt
     3781 cttttctaca ccaaatggag aaacggcttt gatggtcgct gtgccaggaa ctgctgttgc
     3841 ctgtgtaaca gttacaatga catcatctga tttttcgatt gcttctacct gaggaacctt
     3901 tccttcttcg tagcttcctc ttaaaagtgt ataagtgtac tcattctgtc cgtcactgaa
     3961 gttgtccagt tctttgccgt ttactttgat tccggtcagc ggcattccgg atttcacgtc
     4021 ttcgttgctc tttgccatct tttcttcatc tgatgtctgt tttgtcagag gaaggatgct
     4081 gaatgtctgt gtataagtct ttcctgcgga aatgatgtat ttgctcagag gagccggacc
     4141 gcagcttgca tttccaagtc cgcgctgcat caggtcaaca gtcagaatgg tattttcctg
     4201 taccggaacc tgatatggat gacggtagga tgccagatct tctgctttat aatgcagggc
     4261 tccggattcc atttcgctgt cagaagatac caggatacct gttccgtctt ctgctgtcag
     4321 tgctgtccat cttacacctg tgtggtttcc gttttcctga ggttttacat attttttttc
     4381 aaactgttcg gttacggtgc tgttgtatat accaagtttt gcaccggtgt tacggtcaac
     4441 atagtttgcc tgcggaccat ttccgtagta tgtcagcttc tcgtaatctt ttgcaactgt
     4501 catcttcata ccgattcttg caatatttcc tgcagaagca ctcggtgtaa ccgtattggt
     4561 tactacgatc tcaccattgc catagatcat gtaatcaatg ctgttcggtg tatttacttc
     4621 tggaatgctt cctgatacat gaatgttgac tactttgtct ttcttatcta cagtgatgcc
     4681 gttctcatcc agcgtgaagt tctgtgctgc attcttcatc gctgtggaga aggatgggtc
     4741 gttgctgacc ggtgcacggt agtagttcgg aaccggtccg ttctccagca ggctcttgcc
     4801 gtctactttg tagctggtga tgtaaccctg tgctttgtcg atcacaactt caaatgcttt
     4861 gccgtttgtt ttggatgtct ttccggtaac agtgatcgca tcatcgctct ctgctacgtt
     4921 tactttatcc atgtcagatg cactgatcgt tggctgtgct ttttccggag tgtaagcaag
     4981 atcaaattct tcaaatgcaa cctcatcacc ttcatgtccg taataatcgc ctgcccacgc
     5041 agcatcttct ttcagtgtta cggagagtgt cagtgtatag tcagaacctg ctgtcgcact
     5101 tactttcggg aagtttgcca gtgttacggt agcttctgcc tgcggtgcga tgttcttctg
     5161 ctcctcgcta agctgaccgc ttccgatcac tttgttatct tcttttaatg tccaggagac
     5221 attgtatttg ttcaggttgg tgttcaggaa ctcgtttaca acacggactt ttccgtttgc
     5281 tgcttcaccg tcatcataga aggaaacttc ctgatgtact ttcttgactt catacagttc
     5341 tgcggaaggt gtgcggtctg catttaagat accgtttgca cagaaatcat tatcatttac
     5401 cgtctcgccc cagtctccgc cgtatgcgaa gtatgtgctg tctttgtagt ttgttccttc
     5461 tgatttaatg gaagcagatc caaagtcaat agaataagct acgttatctt ttactgcgtc
     5521 cagattatcg tttgtattct ggatctgttc ctctgtcata acacctttga tcacggctgc
     5581 acggtcgatt gcaccgttga aggttcttcc ggactggtct gcggatgctc cgatggtgat
     5641 gtcgatcgga ttgctggatg catcacaggt tgtcttgttg gttctttgtc ctgttgcaag
     5701 ctgttttcca tcgtaataca gcttgtactc ggtaccgata taagttgccg tcagacgatg
     5761 ccagtttccg tctgtgaaat cagatggaat atctgcggat aaagtaccac cgctccatcc
     5821 gtctacaaag aactcaaatt taccatagcg gtcaatctga aggttgtagc cgtcgctgtc
     5881 tcctctggag ataaacgcct gtgcggattt tgtaaagttc tccgatggtt ttacccatac
     5941 atccagtgtg ataccggttg tgctgttggt tgccagtttt gcactgtccg tcgctgcata
     6001 agttccctgg atcgcttttg tattgttgcg tccttcggta acttttccgt caaatttcgt
     6061 tgcaacaccg gtgttcggat ctgtgatcgt gtaagttgtt gtattttcta cttttgtaga
     6121 aacagactga tcgatccagt cccagataaa tccaccctgt gcattcgggt agttacggaa
     6181 aatatcccag tactctttga agttaccaag ggcattaccc attgcatggg catactcaga
     6241 ctggatatac ggcagcttat tgccggtgtt tgttacctga ctcttgacag aatccacacc
     6301 ccagtactgg gaggaataga tatctaccat ggattttgtg cggtcaccct ttgtatagcg
     6361 gttgtcacgc tcgtatttac ggatacggga cggatcacgc tgcaggatcc actgtgtgga
     6421 attgtagaag cagtagtttt catccatatt gtaagcctgg taagtagcct cattaccaag
     6481 ggaccagatc actacggatg gatggttttt atcgcgctct accatattct gggtgcggtc
     6541 cataacggaa gtattccaga tcggatatcc ggacgggatg ttgctgcttg tcgcaccgat
     6601 gtgggactct gcattcgtct catcacagat ataaataccc agctcatctg ccagtccgta
     6661 catatatgga ttgttcggat aatggctggt acggatcgca ttgacattga actgtttcat
     6721 catcttcaga tccgtcgtaa tatcttcttt ggagattgca cgtccgtcct ggtcatctgt
     6781 ttcatgacgg tttgtaccac ggaacatgat cttttcaccg ttgatctgta cctgttcctg
     6841 acctgcttcg ttgatatctg ctttgctgat ctcgcggaat ccgactctct gaacagtggt
     6901 ctccacgaca ttgccgtttt catcttttaa ctgaacaagc aacatgtaca ggttcggtgt
     6961 atctgcaaac catttgtccg gattggttac ttttttatcg ccggtaaagt gtgcaccctt
     7021 gtcatctgct ttctctgcca cattttcctt tgctgccgga acagtcgctt cgatcaccat
     7081 cggctcagac cacacttttg tcttgccgtc aatcttataa agatcagcag atacggtata
     7141 tttcttgcct gctgctgtat catcaccgag attacggata tctgcatcca gcgtcagggt
     7201 tgcatcttta tacttgtcat ccaactctgt ttttacaaac aggtcacgca tctctacttt
     7261 tgctttcgag taaaggttta catcacggaa aataccgctc atacggataa agtcctggtt
     7321 ctccaggtaa cttccggtgg accagcggta aacttccaca gcgatagtat tttcaccctt
     7381 tttcagataa cttgtaatgt taaactcatc cgttgtgtag ctgtcttcgc cgtatccgac
     7441 tctctgtccg tttacatata aatagaatgc ggactctaca ccttcaaagg atacgaaaac
     7501 atctcttcca tcccagtctg aaggaacggt aaaggttctc ttgtactggc cgacgctgtt
     7561 gtttacggtc ggtgctgtaa cgtttgctcc aaggctgaca ttttcatagt tctgccacgg
     7621 atatctctgg tttacataga tcggcttttc gtatttaaaa ttgccgtctg catccttgat
     7681 cgtctggatg gaacttggaa ctttgatatc atcccagcct gaggtatccc agttctccac
     7741 ataatttttt gcatctgcac cttttgcctg tttttcgcgg tctgccggtt tctgggcaaa
     7801 cttgaatttc caggtaccat tcagagactg tttgtaagcg gactcggatg catccttatc
     7861 cagaacggat ttctcattgg tcaaagcttt ctctgctgac tcatatggtg taaaatacgc
     7921 atgtgccggc tcccggttct cctctacttt atcaatctgg tcgtaccatt cttcgcctgc
     7981 aaaactgctg tcagcagcat ttaccggaag tgtgaaactt ccaatgccgg tcacaagctc
     8041 tccgccgatc atggcaccgg ctaacactac agacaaaatt ttctttctca aatgcatgta
     8101 cattcctcct tttatgcatt ttttcttttg cagccgttcc gtttcccttt cagctgcaat
     8161 tttttctttt tctttctctc ttttcgcaga ctttttgtca ttttgaaaat ctctgcgaaa
     8221 aatgaagtcc cgctgtgcgg gacttccagc accctctcgg gatacatgtt aagtttatta
     8281 tagtacattt ttatgttttt gtctatattg ttactattta tttttatcaa gcgttcgttt
     8341 tttagtaatt ttctacatat tctctgtccg tttattgtct atttcgccag tttcgttgat
     8401 tccatcggaa acatccacgc ttccaggaac ctgttttatc acctctggaa acttacgaca
     8461 ctatgcaaaa tgaacgaatt cccaagaagg cacttttacg tgcccttctg ggaattcgtt
     8521 catttgcaca tacacttttt tctgtataca gtttgtctat tcctttcaca ggaaagccgc
     8581 cttcattttc tctgccactt cctgcagttt ctgtgctctt tgtccgaata tccggacagc
     8641 gagatcattc tgcaccagtt ttgtcacgcc gccatcgcac tccggttcct gttccaggat
     8701 ctcccagata ttctgctgca gttcttcgct gccgccggtc agaaagatat ttgccatatg
     8761 cgtgtannnn nnnnnnaacc ttcgtacata ccgatgcctt ccatatccat cttttccggc
     8821 tcatagcggg tgttgtcccg gtagatcagg ctgccgcttc gccagacgga aactttcgat
     8881 gcatacttcc ggtagcgaaa gcgttccccg gatgcttttc gaccgcagga aatgatttcc
     8941 atcagaaaga atcttgaact ttcgtcttcg agatggattt tcatatcact ttcaaatgca
     9001 gactgtgcat atgggatcac cggctgcgga taataacaga agactgcatt ttttttcact
     9061 tctgccgtca cactccggcg tgccagtcct tctttcatct tatgcacttt atcaaaagac
     9121 tgagaaagaa attccagatt gcttccttcg gaaatctgaa aggtgaactc ctgaacatcg
     9181 ccttccatga ttccggccga agcacacagc ggcataatgg aaattccgcc atcttctttt
     9241 ggaaatggtg tcataacttt ataaggtgca gtaaaggaaa gatcttccac tatggttttt
     9301 ccatcccggc ttccggtgcg gatcgacagt ctggatattt ttccgaattt gttttctgtc
     9361 aaatgttcgt ccgcctttct tttttatttt tcaagaatgc ttcttatatt cttgctgtga
     9421 agtaaagcgg acattcacaa tccgcttnnn nnnnnnnnag atcttccagc aatacacttt
     9481 ttttgatcca ttctactact ttatccacat tttcatcccc gcggatgttg gtaaactgga
     9541 atggacggtc accacgcatt ttctttgaat cacgttccat gacttccaga cttgctccga
     9601 catatggtgc gaggtcgatt ttgttgatga caagcagatc tgatcttgtg ataccagggc
     9661 cgccttttct cggaatttta tctccttctg cgacatcgat cacgaaaatc gtggcatcta
     9721 ccagttccgg gctgaaggtt gcggaaaggt tatctccgcc gctctcgata aagagcagtt
     9781 caatatccgg gaaacgctcc atcatttcat caactgcttc aaggttcatc gaggcatctt
     9841 cacggattgc cgtatgcggg catccgccgg tttccacacc gatgatccgc tcgaccggaa
     9901 gaacactgtt ttttgccaga aactgtgcgt cttcctttgt ataaatatca ttggtgatga
     9961 caccaatgct gtagtctttt gccatttttc ttgataatgc ttcgataagt gctgtttttc
    10021 cggaacctac cgggccggca actccaattt tcacgtatga catacttttt cctcctgcta
    10081 tgacatatac aatctggaat acagtttttc gtgctggatt ccacgaatat caaatcccgg
    10141 tgctgacagg cacaggtcat cttcgctgta agttcggatc tcttccagaa tttctgcaaa
    10201 ttccggataa catccgctca aaagctgctg tcctgcactc tgactgagcg ggatggtttt
    10261 gacacagttt gttaccattg cggaagtctg tgcatataaa taatgataca acgtctcttc
    10321 ttcctcgatt ccaagagccg cacagaaaac tccgtacaca atgcagtgac tgaccgcttt
    10381 tccctttctt gcgtccagat acgcttcaaa aatttctctg tttttcatcg gcatcgtttc
    10441 tgcatgcaat ttttcgattg tttttgcaaa acgggaaccc atttttctgg acgcttcccg
    10501 ctgttcaaaa ggaatcctgg atgctccgag gat
//
LOCUS       DAWDWH010000049        10417 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3290_length_10417_cov_9.105385, whole genome shotgun sequence.
ACCESSION   DAWDWH010000049 DAWDWH010000000
VERSION     DAWDWH010000049.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10417)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10417)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10417
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3290_length_10417_cov_9.105385"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3763..3772
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4204..4213
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5016..5025
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7978..7987
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8431..8440
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9444..9497
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2349 a         2616 c         1822 g         3526 t
ORIGIN      
        1 ctgtctgttt ctgtgtctca aagattctgt tcttgtgctt ccctttcttc tccggcagca
       61 gctttttccg ttcgagttct ttttccagtt cttctttcag aagatagcgt acttctttat
      121 ttccggctgt atcaaaaatt ttcagctgcc acttttctcc atggtcaaaa gaaatctccc
      181 agctactttt ttgattttta ctttctttat agagcagaat tttcgcaaag tctatgttat
      241 cttcgcagat ggtctgaacc tgaaagcctt tttctgttac ttttattgga tcgatcacac
      301 agattggcgg tgtccggtca atcgcaaagt cccggcataa tgttttttct ttttccgaca
      361 accgatgtcc tgcttcatcc tccagaaaaa gcttcagact gtaccttcct tctttttcaa
      421 aaacagcctc cgatacttca taatcatact ccgtgaatcc ttctgctgtt ggcctgtatt
      481 ttttctgata atcttttcct tcttccaaat ttcttgcttt tccgtcaaat atgcattgca
      541 gctcttcctt ttgcattttg ttgacatccc acacccgaaa ttttaacttc tgtccctgct
      601 gtgctgtttt ctgtttcaga aatttttgca tggaagcatc cgtcgtaagc ttcggaccgg
      661 agcggttcac tgcaaaacag atttcctgct cttcctgatt ccctgcaaga tcttccgcat
      721 agattttcag ccgataccag ccatctttca gatatacatc atcgccgctc tcccctgtat
      781 tactgccgct gtttttctta tctttcaatt ctgcctttcc ctttttccag tttacaggca
      841 tgtccaaaag ctgttcttct tttgcggatc ttccggttct tttatacagc tttatgtaac
      901 tcttcttttg atttaaataa aattcttcca gggaaatctt tactttataa tcagaaagca
      961 agatctgatt ctgcggcatc ccctgtatga caatctccgg tgatgtacgg tcgatcacaa
     1021 cttttccgac cggaagatat ctggcatttc caaaggcatc tgtcagtttt acatacggat
     1081 accagattcc ttcttcctga atctcaatct gctttccttc tctccattct ccctgcttct
     1141 gtgtgatctg tccgtcatcc agtttttccc ttgcaagata ccactgaatc tgaacttctc
     1201 cttctgtgtc atcctgcaca ttttctgcac tgcaaacaca gaaatccgat gtaaaaagct
     1261 gcttctgttt ctttccgttt aatctgactt ctgcactcgg tgcttcctca tccacttcat
     1321 atgaaatttc tgttttcttt gacctcgcct gcagctcccc gtttttttgg cgacgctcca
     1381 tcacaaaact ccatcttcct tctccataaa gaggctgtga tacgtttgtc tgattgtatc
     1441 cgcttccttt tgtcggctgt gcttccagaa tagcaccttt tgagatacga taaacctttc
     1501 tttttgaatc atacacacat tttccatatc ttgccctgat ttttatattt tccggatttg
     1561 gatctgtctc tttcagaata acatctcctt ttgcagtata tcgtttatct tccggagcat
     1621 aacaataatt ggcatttttg ccagcgactt cttcttttaa agtcaaagct ccctgatata
     1681 ccagatactg tccattctga tatatcggac tgtctttctg taaaacgttc tcatcaatcc
     1741 agactttcgg catcacaaac cctcccggcg gctgattgct cgcctctccg tttttcataa
     1801 atccactgat ctgaatcgca tttttttcat ccgaaaatct taaattttct gttttgttgt
     1861 ccgcaaaata ccctttgttt gcatcactga tcgacacttg aagaattttc ggcacaattt
     1921 ccacctttgt ctccagagct gcctttctgc actccaacac gtacgtatcc gcatcggtcc
     1981 ctgcaatttc atactccaca tctactcgcc agatcccgac attttttgaa acagcatagc
     2041 cacacacaac cagatcggcg gcttctgcgt ccggtttacc tccttcccac tgtgcccgca
     2101 cttccagatc tactttacgt gaatcgtcaa agatcttttt tccgtttttc ggatatgcct
     2161 cgtagacgat cacttttttc ctgtttttta ctttttctct atcgtgatct tcttcgctgg
     2221 ttttcgtaac tgcttctctc cttatttctg tggcacctgt ttccgctttc actttcaaaa
     2281 tatgaaagca aatcatacaa caacatattc ccatcaaaaa tatcattttg catattctgc
     2341 ccttttgctt actccattgt ttcttttcga ttgctcacac cccgtttcca ttcctgtact
     2401 tcttcctttg taatctcatt tttctgaaac cattctctgt ataaatccat tatctgctgc
     2461 atttcttcct cttctttttt ctgttgttcc attctttcat ttatcgtcgc tttctgcggc
     2521 accttatctt ttgctacacc atctttttgt gtatcatcct tcgtttttat aaatttttca
     2581 atccagtttt ctgcatattg ttctccgcca ttttccgcat aaaaaaagtg atagatgaca
     2641 gataattgcc ctgcgaaacg atgataagct ctcctgtttt tctgaagatc actttccgta
     2701 tagaagagaa gcgacataag ttcctggtgt tcctgacgat ccatctgtcc atcttccatg
     2761 atactttcta aaaattgctg ataaccttct tcccgaccgg gaaataagaa tattgctgat
     2821 ttgtaatctg atatgtcttt ctgacttaaa taagtttgat ataactgttc tcgtttctgc
     2881 tcatcctgaa tggaaatttc gatgcttcca agtacggata cggtaatgat catcagagct
     2941 gaaaacaagc tcatccttct tgtaatgtgc agataatctc tgaatgcttt tttcacttct
     3001 gcaacatttt gaaaccgttc atccggatcc tgtcttgtgc acttttccag aatccgtcca
     3061 agcggagaat aactcttttt tcttttccgc tgcttttttt ctaaaatcca ctgcaatgtc
     3121 ctgccaagag cgtagatatc cgttctcgca tcaagactcc tctgctgctc ctgttccggt
     3181 gccataaatc ctctggtacc cataatgcaa aaatcgtcgt tctggaaacc cgccccgtcc
     3241 ggatcaatga gttttaatct tccctctttt gtcagaacca cattggaagg ttttaaatcc
     3301 tgataaagaa tcgccggctg tgacatatga aggcagtata gtgcatcgca cagctctatc
     3361 ccccatttcg ctgcttttct cccggaaatt ctttttttct tttctcctgt cgtaaggata
     3421 tgctctttca ggctctctcc ttcaagatac tccataataa aacagcacat ctcctgctcc
     3481 tgtaaaagat cataaatttt cacaagtccg gaatgctcca tacttgccca tgcccgtaga
     3541 agagctactt tctgtgaagc cgcaaagatg gcttttgccg ctcgcaaagc accggttgcc
     3601 tggtgaaaca caagccatac tgctcctgtc ctcccctgtc ctatcctgcg gatcagaaaa
     3661 taaccatggt gattcaacag atgttccatt tgctttttgc actgcacctg ctcctgattt
     3721 tgttctttcg ccctcatatt actctgatat ggcattctca aannnnnnnn nncaagtctg
     3781 agccattttt ttaaagtttt cgcatcttgt tgttttaact tttctgttcc attataaaaa
     3841 gtgtaaaact ccggcattgg aagttttacc atttcttttc tgtaacgttc tcttaccggt
     3901 acaagttgct catatgtcct gacaatatac agaagactcc tgagcggcat atcatgaata
     3961 actacatctg cgtatacaat ctgcttttga ttcttttgtt tttgcataag actgcccctt
     4021 tcttttgttt gtacgaagca gtctgtccga attcagtata cacaaaccca agcagttttt
     4081 tcaagccgct tcgcacaggt tccttatttt cttttctcat tggattttcc tgccgaacgg
     4141 cattgaaaaa cagatttttc tttgatatgt tgctattata atgtaaaagt gatataatgt
     4201 caannnnnnn nnnaacaata agtttttatg tatgcaaatt atactccgtc agctgccagc
     4261 tccagtcgaa actgcagatc ttctgtccat tctttccatt ttggggggac tgtgattttt
     4321 gcctgaaaat gtactttttt tccttcacaa agtttttctg cacagtaaaa atagccatct
     4381 ttttcgcaat agaaccagtt ttcattcatc tgaatgtttt ctaacagatc tctctgctgc
     4441 ggtgccatga tcccgtctgc cagtactttg acccgcacct gcaccttcgc ttcacttttt
     4501 tcaacggtga cccaggcaga tttttctatt tgctctcccg gctttacttc gatattctcc
     4561 gtctgttctt cctcttcttc cagtaaaata tgtaccggcc cgttcattgt gatatgacgg
     4621 ctcctggctc ctgccacgta tccgaaaagc aaaatgcctg cggataacac tccccacaat
     4681 acagccaaaa atctatacat ttttttcttc ttatctgctg ccggttcttt tcgatgatac
     4741 cgccttgttc ttcgttttga atacaagatc ctttctttca aaatcatagc atgtttcctt
     4801 tcccggcatg ttttctttct atgcctgtcc tgttttacat cctatgtttc caaaaagggc
     4861 tgcatacttc ttttttgttt ttttgccgat ctttcgaacg cagaaaactg caaaaaaaga
     4921 gacaccatga cgcttttcgc cacagtgtct gatttttatc ttatctccga ctaagataaa
     4981 ggctccgcga gttccgtgtt ttacacttcc tacatnnnnn nnnnntccta cattctctcc
     5041 ggtgatgtga atccaagtac atggatgcag ctctcaagca catctcttgt cagcatcaga
     5101 agtgccaggt aacctttttt cttttcttcg ttctcttcgc ttaagatctt gacttcatga
     5161 tagaaatgat tgaagtcatt tgccagctca taaatataag cacagatttt atgcggtgcc
     5221 agttcttcaa aagcattttc cataactgca ttgaatttgg acaatgccag catcagagct
     5281 ttctcgctct cactgtttgg tgtctggatg gtgattcctt ccagagaacc gccttctgcc
     5341 tgataacgtg ccaggatgga tttgatacgc acgatcgtat acagaatgta tggaccggta
     5401 tttccttcaa aggaagtgaa gcggtctacg tcaaagacat agtctttgga tgcctggttg
     5461 gaaaggtctc cgtatttgat tgcggaaagt ccgacgatct gtgcagtctt tcttgcttct
     5521 tccggatcgg tctcgtggtt ttccatgatc ttgcgataca tttcttcgtt gatctctttg
     5581 atcaggtttt ccagacgcat aacgccgccg tcacgtgtct tgaacggctt tccgtctttg
     5641 ccgttcatcg tgccgaagcc aaggaaggta agtctggtat catcctctac cagttttgcc
     5701 ttctttgcac agcggaaaca ctggataaag tgcagttcct gacgtttgtc taccacgtag
     5761 aggatctcat ccggatgata cagtttcatt cgctcgatga tcgtcgcaag gtctgtcgta
     5821 ttatagaggg aagccccgtc agatttcagg atcatgcacg gcgggatttc cttggtgtca
     5881 gactcttctt tgacatctac tacaagtgct ccgtcatcga tataggcata accgttatct
     5941 ttgaggtatt ttaccatatc cgggatgtac ggctgtgcat cggactcgcc tttccagaga
     6001 tcaaaagata catccagacg ttcgtaattc ttcttcaagt ctgttacgga tacattcagg
     6061 atgtgtttta agataccctg ataacctctg tgaccgtgct gaagctgata ggttgcctgc
     6121 atggcagcct ctttataagg ttcatcctct ttggaacgct tgcttgcagt cgggtagatt
     6181 tcttccagct cactgatcgt aaatggcggc tcttccggat attctccggt ataactctca
     6241 tcaaaataaa ccagatccgg ttttctctca cgcagctctg tgatgatcag tcccatctgc
     6301 agaccccaat ctccaagatg tacatccccg atcatctcat ggcccataaa acgtccgaga
     6361 cgtttgatgc tctcaccgat gatcgcagaa cgcagatgtc cgacatgcag cggttttgcc
     6421 acgtttggtc cgccgtagtc caggatgatc ttctttggat gctctgcttt ttctacggaa
     6481 agattttcat ctgcctgcat ctgttttaaa tactctgcca gataatctcc ggaaagcttc
     6541 aggttgataa agcccggact gactgcctgt acttcctgga aaataccgtt ttccttcagt
     6601 tttgccacaa catcatttgc gatcatgatc ggagcttttt tgtaggtttt tgctgctgcc
     6661 agtgcaccat tacactgata ttcacataaa tcaggacgat tggaaagcgt cacttttgca
     6721 aaatcggcac tgtagccggc ttcttcaaac gctttgacaa cctcttctga aatcagatcc
     6781 aatatttttt tcatatggtt ttcctttcct ctaaactgtt tattttccgg tatcctgccg
     6841 gaaactctgt tttttattat aatgtttcgc tttctttttg taaaggagaa tataagacct
     6901 tctcaagggt aagtcccttt gccggtgccg taaatcctgc cttttcgcgg tctctgactt
     6961 ccagcagttc tgccacggag gaaagacttc tctttttcat tccaacttcc agaagtgttc
     7021 cggtaagaat ccggatcata tgatgcagaa acccatttcc ggtgtatgtg atcacgattt
     7081 ccccttcttt ttctttcaga tcaatgctgt agatcgtccg caccgttgat tttttcatcc
     7141 tcttataata actgtcaaag caagaaacac tttagacaat ttttcttttt cataactccc
     7201 tcttatttta acatttgcgg aatgcttcgg aaaggcattc cgcttttgtt ttatgataag
     7261 atggaaattc aagtcgaacg cacgtgagac ttggcacgcg attttgatca gctttctgca
     7321 ttttttaata tacggtaata tctttcagct ctttttgcag ttcttctctt aacagttctt
     7381 ccgcttcttc cgaagaaaga tttcgatctc tgttcagggc atttaaaatc atccacaaac
     7441 attcatggat cacaccatcc tgcgaagtaa gtgtacgtgt tttaatgctt ggtatgatat
     7501 gatccatata atagatgccc agatcctgat ttccgaacca gcggctttta tagcttttca
     7561 gggacggatc ttcttcgtat ggcttttttg ccgtagtcat aagtcccatg gagttgagtg
     7621 tccttgcccc ttcggtcgaa agtgtcgcaa atttcagaaa ttcccacgcc agacgtttat
     7681 ctttgcaggt ttttgtgatc cccatacttg tcccacccca gttggcattt ccctccggtg
     7741 cactcatcag cccccagcgt ccgagatttt tctgctgcgg atcgcttgat tcaatgctgt
     7801 atttgacgga ccaggttgca caggcggtaa aaatgtgccg gtcttcccga agtgcctcat
     7861 tccatgccgg tgtccaggtg atcagattat ctgaaatatg ctcatcacgg aaacgggtaa
     7921 ccagcgacag ggaacggccg aaaacatttt ccacattgat ctttccatca tttacccnnn
     7981 nnnnnnngac tgttcctgcg taaactgccg gatatcctca agtgaaaacc acatatagac
     8041 ctcgccgttg cttttttcgt aaacttcttt ccctttctgg ataaaggctt cccaggtcgg
     8101 catcatcttt tccagctctt ccggatcgtc tgttccaaga tatttttttg caagatcttt
     8161 gcggtacgcc agccctgccg gtgagagcga ctgttcaatc ccacagacat ttcctttgct
     8221 gttaatcata tgcgggtgca gatattcata aacttccgaa aggcggaaat tgtatggttc
     8281 ctgctccagt ggctcccaca tatcaaattc aaacacttca cctctggagt cgatgacaga
     8341 ccaggcaatg tccggaagtt ctccgccgac gatcagtgct gtttcatatt tttttggcat
     8401 atctttgttt tcaacggaag tatactcaaa nnnnnnnnnn tcatctttct ttttcctccg
     8461 cccttttgac cggaagtgtc agaatgactg tcgttccttt tccttgtgtg ctttgtatct
     8521 ccattccaaa tccctctccg tacaggtagt ggatacggtc acggacattc tccacaccga
     8581 tgtgttttcg cgtcttgttt tctgccatgg tctctccctg cttaaaccgt ttcagctgtt
     8641 ctttgctgac accaatccct tcatctttta ccataacaca gagtttttct ccctgcttct
     8701 gaatgatgat ccagatcttt ccggacgctt ccgtcggcag gatcccgtgc aggattgcat
     8761 tttccacaag cggctggatc atggttttcg gcaccaggca gtcctttaaa tcttcctcac
     8821 atacaacttc tacctggaaa aaatccggat agcggtatct ttgaatatcc agataacatt
     8881 ttgtcagttc cagttctttt tcaatggtgg tctccacatt tccatccccg acttttaaag
     8941 tctcgtgcag cgtatagatc agggagtgta cgatcttcac tacatcctga tttttcccgg
     9001 ccgccgataa atatactact gtatttaata cattatagag ataatgtgga ttgatctgtg
     9061 agagcgtaat gtcatattcc atgtcacgtt tctgcttttc ataggcaata cgttcctgct
     9121 ttcctttgta cagctccgtc atcatatcct ggaaacattg atataaaatc tcgatctcat
     9181 cgccggtatg gacaatgtgg atcggctgct gtttgccata ctccgtgcgt tccatctgct
     9241 ttgacagttc cgtcaccgga cgcaccatat tctccaggat tctggaaaca acgagcagca
     9301 ccagtcctac acaaagcaaa aaggagagca caaaaaactt cagcacaaaa ctgctgtttt
     9361 tcagaagatc tgcattggta aggaacgtac agagcttcca tccggtcccg tctaccggta
     9421 tgcagatcag ataaccgctg ggannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnncct tcacctgcgt cagaaagtac tccaggctca tctccagata
     9541 gagtgtcgct ttcagatgac cgtaacggcg tttgtcatac attgggatcg cataacaaat
     9601 ttttcttgta tcatttccgg agtcactgtc ataataggga tcggaataga tccattcctt
     9661 ctttgcagtg tattcttcca gttccgtctg ctttaatttt tgcctcagat aggcactgtc
     9721 cctggagctg taactgcttc cgaaaaaatt tccgtctgcc atctgaagaa cagacccttc
     9781 cagataggta cgcagattat tataaaacac cagccgtttt gtcaccttat catactggtt
     9841 ctgcctgcgg aattcattcc ctttgacgtt ctcttccaga aggtactgca gctgttcatc
     9901 caccagaata ctctgtgcaa accggcggtt atcctccgcc tgaaatgcaa tctggctgcc
     9961 aacctgcatc agacggtttt tttcttcttc aagtctctgt tttcgcacca cctgattaaa
    10021 atataaatag ccgatcagcg tactcaaaat catggaacat atcaaaatca gagaaatact
    10081 ccgcaggatt cgccctttta aatgtttttt gtcccttttc tttcgctctg ttttcattct
    10141 ttcctcccta cgttccgcat aaagatctgg ctgaaatact ggcttgtgcg gtagccgacc
    10201 atctgggctg tttctgcgac cgtactgttc tcctctcgca gcagactctt tgcctcggaa
    10261 attcttacat tggtcagata tttcagaaaa gtctgtccgg tttctttttt aaagatctgt
    10321 ccgagatata cccgttcata tgaaatttct caccgagcag ctccaggctt aactcctgcg
    10381 gataattttt ttcgatataa tcaatcatat ccagcac
//
LOCUS       DAWDWH010000050        10404 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3294_length_10404_cov_13.407092, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000050 DAWDWH010000000
VERSION     DAWDWH010000050.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10404)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10404)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10404
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3294_length_10404_cov_13.407092"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    344..353
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3230 a         1960 c         2776 g         2428 t
ORIGIN      
        1 tcctggcgat caccttcaat atttccgggg gattttcgag tctgtttcag gcatttcacc
       61 atgcagcatt tcaggttgga tcgatcatca cgaccaccgg attcagtacg gtggatttca
      121 accagtggcc gggtttttcc agaacgatcc tggtgacact gatgtttgtc ggagcctgtg
      181 cgggaagtac cggaggcggt atcaaagtgt cacgtatcct gcttttgtta aaaggcatcg
      241 gatgtgagat ttcactggca ctgcatccaa gaagcgtcaa gcgtgtgcgt atggaaggaa
      301 gaacggtgga gaccgaagtt atccgttccg taaatgcata tctnnnnnnn nnngaaagac
      361 ctggttacca actttacggc cgtggcagcg actttgaaca acgtcggacc gggacttgga
      421 ttggtcggac cggccggcaa tttctcagga ttttccaatt tgtccaaact ggttttaagc
      481 tttgatatgc tggccggaag actggagctg atcccgatgc tgatgatttt tgtaccgtcc
      541 gtatggagtg tgaagagaaa gaaaaaataa gatgcatgta atgaggtgaa ccgaatgttt
      601 ggagaatgtc atgctcacat ctttctaaat ggcagaaatt ataaagaagc agtggcactg
      661 cataaaaatc atgtcagcga agaggcgatc cggcaggcat ttgcagagta tcggaaacgt
      721 gggattacat ttgtaagaga tggcggagat gccctgcatg tgtccgaata tgcgtcaaag
      781 atcgcaccac agtatgggat cgattaccgc agcccgatgt ttgccattca tcgcaaaggg
      841 cattacggca ggatcgtcgg atgtgcctgg gaagatctgg aagaataccg agatctgata
      901 aagaaagtaa aagatctcgg cggagatttt atcaagatca tgatctccgg gatcatggtt
      961 tatgaacagt tcgggcagat gtcagaagag ccgcttgcac cggaagaaat ccgagaactg
     1021 atccacattg cccacgaaga ggggatggcg gtcatggctc atgtcaatgg ggcggatgcc
     1081 gtacgtgccg cagctcttgc gggggctgac agcatagaac atggcaatta tatcgaccgg
     1141 gactgcatcg atgccatgaa agagaagggg acggtctggg taccgacgct tgtgacgacc
     1201 ggcaacctgc ttggctgtgg aagatatcct caggaagaac tggaaaagat ttttgattct
     1261 gccagggcga atgtaagctg tgcctgggaa aagggcgtgc tgcttgcagc cggaagcgat
     1321 gccggagcct acagggtgct gcacggtcag gggctggtgg atgaatatca gatattttgc
     1381 gatttatttg gtgagacaga ggaactgaaa aaccgtctta aagaaggcga atctgtgatc
     1441 cgccggaaat ttcaggcgga aaagtttgga agttaacttc caaatctacc attattgacg
     1501 cagcaagtgc gtcatgaagg aggaaaatat gatagcacag aaattgatca cagttggaga
     1561 gaatgaaaat accgtgttca gaagctgcaa ggatggaatc catacaaatg tgtttgagac
     1621 aatctgtgca tttctgaata ccagaggagg aacacttctg ctgggagtag atgaaagcgg
     1681 tgaagtatat ggatttaaag aacgggcaat cccggatatc atccaggcac tgaagatggc
     1741 aactcagaat gcagacacct tccagccgcc gtttgagatg gaaccggtgc gtgagatcgt
     1801 ggacggcaaa tatgtcctgg tgttgaatgt tccggaaagt gcgaatgtcc atgctcttcg
     1861 cggaaaggtt tttgtaagaa gaggaacaga aaatatccgt gtggaacgaa aagaagagct
     1921 gacaagactt tatgtgcaga aacaggagat ttacacagaa cgcaaaatct atccgtttat
     1981 gcgggaaaac gatctttcag acagtctgat cgagagggca aaagcactgc tgctgatgaa
     2041 aaacgaagag catccgtttg gcaggctgga aaatcatgcc cttttaaaag aaatgaatct
     2101 ttccggtatc aattatgaga cgggagaacg tggtcttcgc cttctggcag gccttctgtt
     2161 cggaaaagat atgacgcttc gcaatctttt cgccaataca gcagttacct gtgagcggca
     2221 ggtggacgga gaaacacagc gtctgaatat ggagacgaat cttctggatg aggcagaaaa
     2281 actgatccgt tttctgcagc cggcagtcgg agaagagacg gcgaaagcat ttgtcagtga
     2341 atttctcttg tccagagaat atacgagtgc gtatccggca aaaatcacgg catcatcgca
     2401 gaagcttacc gcagagtttg cagtcagcat gggacgtttc ggaaatccga tgctgaagaa
     2461 atatttctgt gagtttggta ttgcatcaga agaggacagc tatggactga caaaagaaga
     2521 acacgacggc atctgccgga tgacctggca gaaagaaaaa tccggcaaaa caagacagga
     2581 actggtaaaa gaagctgtgg taaagacacc tttggcagcg gaaaacagag cattttcaaa
     2641 aacggatgta agtgatccgc aggaaaaaga aagtgctgac ggtgaacgtt tcacgccgca
     2701 ggagcgtcag atgcagatcc tgaaaatgat ggcagagaac gaacgcatca cgatcagtgc
     2761 tatggtgcag gagcttcagg tcacaaagcg taccattctg cgtgatatcg acaaaatgaa
     2821 aaagcagggc tggcttaaaa gagaaggatc agagaagaac ggaagatgga ttcttcttgg
     2881 ggaagcaaaa gaacaggtaa agcaggatta actgacttgg aagatagaaa agccagatca
     2941 gctttccggc gatgtgcgtg cagtaaaaag caagagaatg cggaagatca ccacagaagc
     3001 ggggcagatt gaaaaatccg acattcagat cgcaggtaag aagcatgaca aggcagattc
     3061 gcagcagagt gggcgtattt tgtcgtttca ccgtaaaaga actgcacaga ttccaggtca
     3121 gcagtgcgat atagaaaaag gcggccgccg taaggaggct gctttttata tgccagaaat
     3181 aagagagcaa aagcaggaag aacatgccag aaaagccgcc ggcggtaaaa gagagcaaag
     3241 aaccgttttg cagacggcga tggcagtact gcactgcgat aaaaaacaag attgccgtaa
     3301 gttcaaaggg agtcttcaga aaaagaacat cggctgtgaa ggaaaaaatc agggataata
     3361 aaagaaattc tgttttcacg ggcttgctcc tttcaaaaac aggttatgcg atgcatgatg
     3421 tacacctcgc agcaagaatg ccagagacat ttctgaattc agtatgtaca gaaaaatgac
     3481 agatatactt gcgggatgaa aagttttgag ctatactctc aacttagaaa ccgatgagga
     3541 ggcgaaagaa atgcagaagg cagcattgat ccttgaggga ggagcaaaaa gaggggtttt
     3601 cacatccggc gtgctggatt atctgatgga gcaggagtac tatattccgc atgtgatcgg
     3661 agtttctgcc ggttcctgca atgcagtgga ctatgtttca aggcagcccg ggaggacaaa
     3721 ggcatgtatg atccgttccg gcgaggacgg gaaaataatc aattttcaca actttttcca
     3781 gacgaaaagt ctgctggaca tggatctgat ctttgagcga gatccgaaag aactgcatcc
     3841 ttttgatttt gatacttact ttgcatccga tatgacctgt gagatcgtgg tgacaaactg
     3901 cgagagcggt caggcagaat attacagcga aaaagaagat ggagaccggc tgatgcagct
     3961 ttgcagagcc tcaagcagta tgccgcttgt aacaccgatg gtagagatcg acggggtgcc
     4021 gtacctggat ggaggcattg cagattctat tccgctgatc cacagcatgc gaagcggtta
     4081 tcgcaaaaac gttctgatcc tgaccagaag caaaggatac cgcaagacca tatccaaacg
     4141 ggatattctg atgtatcaga cggtttttaa ggattatccg aatctggcga gggcactttg
     4201 ccgccgggca catcagtaca atgaagtcat ggaactggtg gaacgctggg aggaagaagg
     4261 aaggattttt gtgatccggc cgcagggcaa gaccgtctca agactggaat ccaaaccgga
     4321 agttctggaa gatttctatc agcacggtat agagcagatg aaagaacatt tcggggcaat
     4381 gcaggatttc ctggaaaaat aacagagaaa atggaacgat tcttacaggt tcaggcatgt
     4441 attgcaaaga ctgtaagaac atgggagaat ttatgttagt ggaacgtttt tatccggatg
     4501 aatatctgga ctcaacatac agcattgatt ttgccgaact ggcaaagaaa ggctatcggg
     4561 gagtactctt tgatatcgac aatacactgg ttccgcacgg agcaccggca gatcagaaag
     4621 caaaagccct gtttgcacat ttgagggagc ttggcatgga ttactgtctg atttccaaca
     4681 atcagcttcc gcgtgtgaaa ccgtttgcgg atgccatcgg agggaaatac atcgaagatg
     4741 cccacaaacc atccacgaaa aattatcgga aagcaatgca gatgatgggt tgcaccgaaa
     4801 aagacagcct gtttgtgggg gatcagctct ttaccgatgt ctggggtgcc aaaagagcag
     4861 ggatgcacac cattctggtt aagccgatcc atccaaaaga agagattcag atcgtgctca
     4921 aacgttatct ggagaagatt gttctgtatt tttataagaa agacagggaa aaagaatgac
     4981 agaaaaagtg acagagctgt taaaaaagaa aaatctggat gcgattctga tatccgatgg
     5041 atataatatg cgatatttaa gtggttttgg cggtgcgacc ggttatcttt atatatctgc
     5101 cgatcagaaa gtgctgatga ccgattcgcg ttataccacg caggcaaaac aggaggcacc
     5161 gaagtttgcc gttgtggaag ttagcactga gaagggttac cgggagctga tcacagaatg
     5221 tatccgaaaa gatcaggcaa aaacgatcgg ttttgaagat cagcagatga tctgtgcaga
     5281 ctttaaaaag atcagcagtc cgacaaaaga cgaagcaacg tggatggagc ttggcgaaga
     5341 agtcaatctt cttcgctgca tcaagacaga agaagagctt gaaaaagtcg caaaagcaga
     5401 ggcgatcgga gatcaggcat ttcagaaaat cttaggagac atcaaaccgg gtgtgaccga
     5461 acttacgatt gcggcaaagc tggattatta tatgcgggaa cttggggctg aaggcaacag
     5521 tttcgatacg atcgtggcat ccggacttca ttctgccatg ccgcatgccg taccgaccgc
     5581 aaaaaaactg gaaaagggtg attttatcac catggatttc ggatgccgat atgacggcta
     5641 ttgttcggat atgacacgta ccgtagtgct tggagaagcc tcttcaaaac agaaggaaat
     5701 ctacgggatc gttctgaaag cccagatggc ggcactggag cagatctgtg ccggaaagac
     5761 gggatcggaa gtcgatcagg tggcaagagg gctcattgca gaagcaggtt atggcgagta
     5821 tttcggacat ggacttgggc acagcgtcgg actttttatc catgaagaac cgagactgtc
     5881 accaaaatgc cacagtgtat tgcagaaaaa tatgacgatg acagtagaac cgggcatcta
     5941 tatcccggaa tttggcggtg tcaggatcga agatctggtc gtgatcacag aaaaggggtg
     6001 ccgaaatctt gcacattcac caaaagaact gatagaactg taaaaaacag ttgaaaaagt
     6061 gcaggtttta ttataaaata agatgagact atgcggacag ccgataacag aagtgctata
     6121 agcggattgc gatgtccgtt tcgaaaataa ggaggaagat cagatggtat cagcaggaga
     6181 ttttaaaaat ggtattacac tggaaattga tggaaccgta tatcagattc tggagttcca
     6241 gcatgttaaa ccaggtaagg gagcagcttt tgtccgcaca aagctgaaaa acattgtcaa
     6301 cggaggtgta gtagaaaaaa cattccgtcc gacagaaaaa ttcccacagg cacgtatcga
     6361 cagagtagat atgcagtatc tgtacaatga cggagatctg ttctatttca tgaacgttga
     6421 gacatatgac caggttgcac tgaacgaaga cacgatcggt gatgcactga aatttgttaa
     6481 agacaacgaa atggtaaaag tatgttccat caacggtgat gtatttgcag tagaaccgcc
     6541 actgtttgta gaactggaaa tcacagagac agaacctgga ttcaaaggtg atacagctca
     6601 gggagcaaca aaaccggcaa cggttgagac aggtgctaac gtaaacgtac cgctgttcgt
     6661 agaacgtggc gacaagatca aaattgacac aagaacaggc gaatatctgt ccagagtata
     6721 agaaagatca tggctttatg ccgacttgaa ttcaaagaag atgctttggc ttgaacaaac
     6781 tgctgcagtg tattgaaaga tatgctgctg cagttttttt agtcggagaa gacttccttt
     6841 tctaaagtag cgggcaacag atttgtctca gtgggagtgc agtctccggc taagataaac
     6901 gctccccggg aatgtctccg gcatattctt tattcttgtg caggggtctg gaatatgata
     6961 tgataaaagc ataggaattg ccctgagaca aagtacttcg caggcgagaa aaggaaagaa
     7021 aaacaaaaca ggcggaaagg agaaaaagga atgaaagagc gtaaaaaaag agaacataca
     7081 atggtgcgta agctgtttat gctgatggca gcttttgtac tgatcctgtg tatgaaaaca
     7141 acgacgtatg cagcatcaac aaaaaccgtc aatatggaaa gtgtcggctc cggtatgtat
     7201 atccatatcg gcactatgaa acgtggaaaa ggaactgttt accacagggt aaaagtaaag
     7261 aaaaaaggca tcctgatcgt tgcaggggcg gcgatcgcag aagacggaac accgtatggt
     7321 atggcagttc gtctgtgtga cagtaagaaa cggtctctcg agccatataa aagaagctat
     7381 gtcagcacgg aagagtttat cgtttacgga gtgaatccgg gcacttatta tattgaggta
     7441 aagaatcaga gttattattc tctttcggca ggagtaaaga aagtaaaaga ccttggagga
     7501 gacagtaaaa agacagcgta tcagattgct cataagaaaa gagtatccgg acttgtgaca
     7561 gcaggcgaaa aagcaggaag tgcagactgg ttcaagttca agcttgcgaa aaacagaaaa
     7621 gtcaagatcg agctggcagc aatcggtacc gatgcatttc gattctatct ttacggaccg
     7681 ggttgcagaa atggaatatg catggcttcc ctgaaaaatg acgcaaaaga ttttcagaaa
     7741 aaggttgctt tgaagaaagg gacttactat atcaaagtgg tgcgtgcgtc aaagaaaagc
     7801 aatgcatccg gattttatga tgtgcgatgg agtatgcagt agcagctgcg tagaaaaata
     7861 tgaatgtgca aatgaacgaa ttccccgaag gcacgctctc gctacctcac attggcagcg
     7921 gtacgtaagc gaccaaaata cggtcgcaaa gtaccggcca tgttcgagta ccttcttggg
     7981 aattgttcat tttgcacatt gcccaaagtt cttggctggc caggggcatc tatgcagaaa
     8041 gatatttccg gccggaatcc gatgaacagt gcatgtgaaa cagcacagcc ataaccgtac
     8101 gaaaacaagt cgcactgacg gtagcaaggc ttccgtcgtg aggtttgcga tgctgccgcc
     8161 ccgaagagcc gctgtttgag cagcaaagct gcgagtttag gcggaagggc ggctaatcgg
     8221 cgagcgaacc gaacaggaaa cgcagcgtgt cagcagattg ttttcgtatt gttatggcgt
     8281 tctgttttct atgtactgct ttttctattt tgcacggtat ttttttattt tacatagtgc
     8341 gttctgatta gtcatatttt ttatgtatca aaaaaatatt taaaactaac ttgcaatatg
     8401 ataaaaatta tgctaatatg taacggtaaa tttacaaaga aaaatccatt caaccgggct
     8461 tttctgcatg gcaggaaagg actgtgtatg agttacaagg aatttatcga aaatcttcaa
     8521 aaggaaatag aaagccgcat tggggaagcg acgattctgt ttcataaagt tacgaaaaat
     8581 aatggagtta ccaaagactg ttttctgatc cgggagaacg attatgatgt atccccttct
     8641 gtttatcttg aagattacta tcatgcgttc agagatggtg cgacgattcc ggagcttgcg
     8701 gagaaagttc agcatatcta tcaggagtgc agaaacaata catcgctgga tgcaggacat
     8761 tttctgaaat ttgataatat aaagaacgag atcttctgca aaattatcgg cagagggcga
     8821 aacgaaaaac tgcttgcgga agtaccgtat attccttatc tggatctcgc ggttgtcttt
     8881 tattacgtgc ttgagaatga gatgtttggc agcggaagta tcctgatccg tcaggagcat
     8941 ctggatttct gggaggtcac aaaagaaaaa ctgtgggaat atgccaggga aaacaccaga
     9001 gaaaagcagc cgatgcatgt atataaaatg tcagagctga tcagggagat gcttccggaa
     9061 ggggaaaaac tggattttct gaaggaagag attccgatgt acgtgatgac gaatgagaaa
     9121 cgctgtcttg gggcatgcgg cattttgtat gaccatattc ttcaggaagc agccagactg
     9181 ttagagggat cgttctatgt gctgccaagc agcgttcacg agtgcattct ggttccggaa
     9241 gaagcagcac cggaggccgc agagttgctt tccatggtgc gtgagatcaa tctcacacag
     9301 gtgcctttgg aagaggtttt atcagatcag gtctaccact acgatgccaa aacgcatcat
     9361 ttaacgatgg ctttccccca ctgattactg aaaaatctcc attttgcttt actttttgaa
     9421 aaaaacatgt tatagtaaaa aagaatcttt ccaaaagaaa gattttacat gagtatgtgc
     9481 ggaagcattc atgtataaaa tgtgcacctg tagctcaggg gatagagcaa tggtttccgg
     9541 taccatgtgg cgggggttcg aatccctcca ggtgcgtttt tttatatttg aaaaaaccgc
     9601 aacagcaaat ggaggtagaa atttgggtgg tttaaaagaa tggctgtcag ataacctcag
     9661 atatatcctg gtcggactgg caatcgtact ggtcggtatc gtcattgtgt ttgcagtgaa
     9721 actgaccggt ggttccggaa agaaaacatc agattctgag acaggaagcc agacgattga
     9781 gaccggcaca gagacacagg ctcagacaga gacggaagag gctttgacag aaaatgatct
     9841 gaccgttctt gagactgtgc agaaatatta tacagcaatg ggcaacaaag acctggatac
     9901 gctgtcaggc atgacagagt ccttaagtga cagcgataag cagaaaattg agagttctat
     9961 catacagagc tataataata ttgctgtgta ttccaagaaa ggtccggtgg acaattccta
    10021 tgttgtattt gcttattatg aagcaaaagt aggcgatgtc gaggaactgg ttccgagtgt
    10081 ggtctgtctg tatctaaaac cagatgagaa cggcggattg tgtgttgctg actggaagtc
    10141 tgatcaggac accaacgatt atatcgagaa agtaatccag aattccgatg tacaggctct
    10201 gatcaaagat gtaaatgaca agtatcagga gatcgtttcc ggcaacgatg agcttcagaa
    10261 gattgtccag gagatggatc agccggagac agagattgcg attccggatg cagaagatag
    10321 cggtgtggaa gccaataaag tcatgaaagc aaaagatgtg ctgaatgtcc gtgcagattc
    10381 ttccgaagat gcagagatta tcgg
//
LOCUS       DAWDWH010000051        10331 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3314_length_10331_cov_11.951538, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000051 DAWDWH010000000
VERSION     DAWDWH010000051.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10331)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10331)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10331
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3314_length_10331_cov_11.951538"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2162..2171
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3132..3141
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3992..4001
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7484..7493
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3539 a         2053 c         2611 g         2088 t
ORIGIN      
        1 tctgttatac gaactgaaca aacgtatgta agatacgaaa aattcagtta tatcaaggac
       61 tttcagcaaa aagagaaaag tggatttgat tgacaattat cggaaatagg ggtacactgt
      121 tggcgtaata gagggctaat ttcataggag ttttgtggtg agattgcgtt tatgtgatct
      181 ctttaaagga atccggtgag aatccggaac agttattccc tgctgtatgg gggacgaaaa
      241 agattatttg ccactggtta gtcaaactgg gaaggcatct tgagtaggag gaacccaagt
      301 cagaacacaa acacaaaacc ggtttatcag caacgtccgc aggcatagcg gaggtgtatt
      361 ttttgtcccc ttttgcccgg tgtctgaaaa ttatgaaatt ggagaacaca cagaaaggga
      421 aggaggaaaa cgaaaaagga aaacacagat gtgagcatgt gtaaaataaa aaaagcaaac
      481 agaagcgaat cgccaaaaag cgaatgagga ggaacaaatg gaacgaagag aaaaaagatt
      541 tgcttccaga attctggcat ggtttctgac ctttttgatg atcacaacca tgattccgac
      601 aagtgcattc gcagcactgc cgggacagga cacgggagca gttgtttcag aggatgcgtc
      661 atcggatgaa acaacgcagg atggtattct tttaaagaca actttgacgg acaagcttaa
      721 ggtaaaagga cagcgcaagc tgttcgatgt ctgggcaaga gatgctgagg acaacacgct
      781 caaaccgaca gcaactcttg acggacaggt actttcggca acctggagtg ataatgtaaa
      841 gacaagcttt acgctggatt tccagggcat ggaagaagga gagcataccg ttgttatttc
      901 cgcagaagac ggaaaaggag catcgaaaac acagacttat accgttattt atcagaaagc
      961 caaaaaaggc gaatttatcg gttatgcaac ggtcagcatt gaagccttta caatctcaaa
     1021 aggttatatc gtagaaccgg ttctgatgcc ggtttacgaa ggtgacaatg cagcgaaagc
     1081 atttgtaaac ctgatccgtg aagaaggcta tgatgtagaa tacaccggaa gtctgaccag
     1141 cggtttctat ctgtcccata tcgtaggatc taatgcaaaa aaaccaaaga atgcgacaaa
     1201 gaaactggat cttgccggtg cagcacttga gccgcatatg gcagaaaaac ttccggatct
     1261 gatgtttgac gccgacggcg gaacagaagg ttctcttggt gaatttgact acaactacat
     1321 gtcaggatgg atgtactgtg taaatacggt attcccgaat gtaggatttg cagattatta
     1381 tctgtctgac ggcgatgtca tgcgtgcaca gtttactctg aagtacggcg gtgatatcgg
     1441 aggttccggc ggtatgggcg gcggctatga tgattcttac agcgtcgcaa acaaagatgt
     1501 gctgaccacc actctggcaa agatcaattc tgcaccgaac agtgccgagc tgaaggcaga
     1561 tccggatatc gcagcaaaga tcacagaagc aaaagaagtt ctgcaaaata ttgttgcaga
     1621 acagagtgaa gtagataaag taaacgatga actgaacaaa ctgcttggcg gtgagatcac
     1681 aagtatcaca ctggatcaga gtgagatcac actggaaaac gaaggtacca tgcagttaaa
     1741 tgcaaaagtg gagctgacag gcaaaccgac cgagccaatc gaatggaaca gtgaagaacc
     1801 ttctgttgca acggtgaaaa atggtttggt aacagctgtc ggtgcaggaa cgaccacgat
     1861 cacggcaacc tgcggcgaaa agacagcaac ctgtaaagta accgtaaatg caagtccgct
     1921 tcaggaagtc cggctggata aatcaaaaat ggaaatcctg aaaaatgaca gtgaaacact
     1981 gacagcgaca ctggttccgg caaataccac agatgacaga actgttacct ggagcacaaa
     2041 tgcagaagat gtcgcaacgg taaatcagag cggaagagta accggtaaaa aagaaggaac
     2101 ggctgtcatt acagcaaagg ttggtgacaa agaggccgac tgtacggtaa cagtcaaaga
     2161 annnnnnnnn ncaaagaagt ccatacacag cggatttatt ttgatgacag caaactgacc
     2221 ctgaataaag gaaagaacaa aaccttatct ctgagatttg atccgtccaa tacaacggat
     2281 gcaaaaaaag cgacctggga aagctcagag ccgagcgttg caagtgtttc cacaagcgga
     2341 tacgtacgtg cactgaaaaa aggaacgact acggtcacgg caaaaatcgg tgacatgact
     2401 gcttccattg aaatagaagt tgtagaagtg ccgatgaccg gcgtaaagat cagctatggc
     2461 tctaaatcac tgaaagtaaa acagtccacc tatatgtcag caagctatct tccaagtgat
     2521 accacagata ccaagagcgg aacctggaag agcagcaata caaatgtggc aaccgtttcc
     2581 agttcaggaa aggtaactgc agtcagtgaa ggcgaagcga tcatcacatt cacaagcggt
     2641 gcttttactg cagaatgtta tgtaaaagtt ctggctgcaa atgcagacgt gttcagcacc
     2701 atatccatca ctccgaatca gaaagagatt ggatgtggcg tttctgatac acagaagatt
     2761 atggcagatg taggcaatgg aaccgatccg gtcgtatgga cttcttccaa tccggcagtt
     2821 gtggcagtca gcacagacca gtctttggca gcagcacagt cagcagagaa tacgatgcag
     2881 gttggaaaag aagcagtggt cacagcaacc ggaaagggaa cagcagtgat cacagcaaaa
     2941 tcaggacaga agagcgtaga tattccggta accgttgtag cagccggcat gaaagagttc
     3001 aagatcctgg caggaaccaa tgcagaaact tcttttgcat acgagatcac accggaatat
     3061 aatattacga aaaacaaata tcaggttgtc atcccggatg caattaccaa tgtatatctc
     3121 tactccaaac tnnnnnnnnn nacttgcaga taatgtaact gcaacggtaa cagcacgtta
     3181 cacagcggca aataacggat ctgcgaagaa tattacactg agaagtggtg cgacatcaag
     3241 tgccgcctac ctgaccaaaa aagacatgaa tggcaacaca ctgtatgtag atgtgaagaa
     3301 gggagacgag acaacaactt atgaatttga tgttgttcgt gaatccactc tgaaatcctt
     3361 aagtgtggca tctgcagatg gagaagaagt gcttcatcct ggctttgata aaaatgtaac
     3421 agattacaca gtcagtgtaa ccgacaacaa cgacagcata aaaataaatg cagcagcatt
     3481 tctggaaaat gcagcagttt ctgtcaacgg taccgcagtg acaggagaaa catcagttcc
     3541 tctgagcgga gatgagactg tgatcactgt caaagcagca atggaagatg caccgtctca
     3601 ggcaagagaa tacaagattc atgttctgag aaaaaaatcc gtaaaactga cactgaaagc
     3661 agatccggca gatacagtga tcacactgaa agatgctgac agcaaaaagc tgaaagcaga
     3721 gaacggtgta tacacactga aggcagaaga aacttacagc tatgttgccg taaaagccgg
     3781 atatgttaca aagaaagata cgatttctgt tacagaaaac accgagaaaa ccatcacaat
     3841 ggaaaaagca ccggagagca ccagaaaaga tgtatccgca gcatggaaaa acttccgtaa
     3901 cagtgatgac aacatgggta ttacctctgc aaaaacacca acgagcgagg cgacaaccta
     3961 cgaaaaatgg tttaagaaac tgggaagcgg cnnnnnnnnn ngagtgtgca gatcatcgtg
     4021 gataattccc tgatcgtcat gagtgcaaac catatttaca aactggatct gaatacaggt
     4081 gatatcctgc agacaggcga tatggtagca gcgacaaact ttggatacac accgccaacc
     4141 tatgcagacg gcatgatctt tgcaccgctg gcagacggac gtgtacaggc gttcaatgca
     4201 gagacactgg aatctctgtg ggtttaccag gatacgctga aaggccagtc cttaagcccg
     4261 atcacttaca gtgacggtta tatttatacc ggattctgga atgcagagac aaaagatgca
     4321 aactatgtgt gcatcccggt tacggatgaa gatccgacga acgcacagga agcaaaagaa
     4381 gcagcatggc agtacacaag ccttggcggt ttctactggg caggttctgt ggtacgcggt
     4441 gactatgttg tatttggtac ggatgacggc acaagcggtt cagacggaac cggacatgta
     4501 ttatctctga acaagaagac cggagaagtt atggacagcg tcgatgtaat cggtgaccag
     4561 cgttctacga ttgcttatga caaagaaaca gacagactgt atttcacaac aaaaggcggc
     4621 tacctctaca gtctaaaact gaaatccgac ggaacctttg gtaaggatac gctgaaatcc
     4681 gtacagcctg gaaacatgag caccagcaca ccggttgtat acaacggaag aatctatatc
     4741 ggtgtgacaa gcggaagtaa cttcagcgga acctattcga tcagcgtgat cgatgcggca
     4801 acgatgacag agatctacaa agcaccgctg aaaggatatc cacagtgctc tgtacttctg
     4861 tcaaccgctt acgaaaaaga agacggaagt gtttatctgt atgcaactta taacaacaat
     4921 ccgggtggta tcactctgat ccgtgataaa aaaggacaga caactccgga tgtagaagaa
     4981 attttcatac cggaaaaagc aagacagcag tactgcatca caagtattat ctgtgacgaa
     5041 aacggtacgc tgtattataa gaacgacagt gcatgcgtta tggcggttgc atccagtgag
     5101 gcatatatga ctggcgtaac atcaagtgca gaaggcagca cgatcgataa tggcgtggca
     5161 tttgacggaa aactttccag tcatgacatc gttgtcctga cttcggcaga taagacgact
     5221 ctgaccttta ctgcttccga aggaagcagc attaccatcg atggcgtgga aggaaacagc
     5281 agagagatgg aactgacaga agcaggcaag agctttgatg ttgttgtgaa aaaaggaagc
     5341 gatgaaagaa cttataaatt cacactgaaa cgtgaaaaac cgaataccaa cttaaaagaa
     5401 ctgtatgtca gcgacagtaa tcagtttaac atgggactgg tcagcttaac accggcgttc
     5461 agtaagacaa atcatgttta tcatgcagaa tacagcacaa cacgccgctt cctgaatatc
     5521 tggacagcag cagaagatac aggttcaact tataaactgt atccggtaag tgcagtagga
     5581 aagagcacgg tcatcagcag ttcagaagga aatatcatcc gtactgcaac aaacagtgga
     5641 cacgaccgtt atgcgatcta ttttgcagac aacagtgact gcgcgaccgt ccggatcgaa
     5701 gtaactgcaa aagacggcaa gacgaaagat tcttactatg taacactgaa acagcagaca
     5761 ggaaatgcac cggtgatctc ggcagaagaa ggtgcaggtg tgcgtaagag tgcaacaagt
     5821 gcatcagtaa cattcacagc aaatgaatac ggtacgttgt actataaagt attgccggaa
     5881 acggaaactg caccggaatc cattgataca acaggtgaag gcatccaggt tgaagcaggt
     5941 gagaatacac tggaattaac cgatattacc gatgcagcat acaaagtcta catggtactg
     6001 aaagacagtg acagtacacc aaagacaagt gaaatgctga ccgtcagagt ggcatctgta
     6061 gaagaactgc tggcaccggc aaaagaagca gccatcacag agctgaacgt atacaaaaat
     6121 gcagaggatt accgtgatgc tgaaaaagca aagatcacaa agatccttgc aaatgcagct
     6181 gttttgatca acaatgcaaa atcagcagaa gaaattgccg aacagttaag cgatgcaaaa
     6241 gcagatctgg acaaactgaa aacaagtgca gaacttgcag cggatgaaaa gacacagacc
     6301 gatgcagatg cagtaaaagc actgatcgaa gcacttggag atgtaacact cgagaaaaaa
     6361 gaaagcgtag aagcagcaag aaatgcatac gatgcacttt ccacagatgc gaagaaactg
     6421 gtttccaatt atgatgttct ggttcaggca gaaaagacca ttcagactct ggaagcaaag
     6481 aaacagcagg aagaactgaa aaaccagatg gatgcagcgg cagcagcatc cgtaagcagt
     6541 gcgatcacag caatcaaaga agtaacgctg gataagagag gtgtggtaga agcagcaaga
     6601 gccgcataca acacactgac agaagatcag aaagctctgg taacaaacta cacagatctg
     6661 accgcagcag agaaccgcat tgcagaactt gtacaggagg cagcagacaa agcagaagca
     6721 gacaaggcag agcaggcaaa acaggaagct ctgaaagcca aagcacagcc ggtagtcgat
     6781 gcgatcgcag caatcggaga agtaacactt gacagtgaag aagcgatcac agcagcaaga
     6841 agtgcatatg aagccctgga agcagatgtg aaagaaaaag taacgaacct gtctgacttg
     6901 gttatcgcag aaaaagacct ggctgaactt aaagcagcaa agaaagcagc agaagacctg
     6961 aaagctcagc aggaagaagc aaagagacag caggaagaag caaagaaagc acttgaagag
     7021 gcacagaagg ctcaggatgc aaaactgaaa gagcaggaag aaaaactgaa agcagagaaa
     7081 gacaagtatg gatttaatga gaagacaact ttgaaatctg caaaggtgaa aggcaaaaaa
     7141 gtaactctga aatggaacaa ggttaagggt gcaagtggat acgaaatcta ctgcaagact
     7201 ggaaaaggca agtggaaact tgtgaagacg ctccataagg gcagcaaggc aagctacaaa
     7261 ctgaaaggaa aagcaggtaa gaaatatacc tacaaagtac gtgtattcca gaaagtagaa
     7321 aataaagtga tctacggtca gtactcaaat accaagtctg caaagattaa aaaataacaa
     7381 ttaaatttcc gtatgctgag agcataaaaa tatgaaatat atgcttatca gcaaggaaga
     7441 tggatgatca ggggggcaga aatctgcccc cggatcacca ttannnnnnn nnngagcaga
     7501 aaaacaaaaa aatgtggtta tagaaaacgg attcttgcag gagttctgtc ggcagttgta
     7561 gctctgtcat ctgtccagat gcctgttact gctgtccatg cggcagcgtg ggatcagaat
     7621 aaattttcag acagtctgaa gctgcgtttc tggaacaatg aaaagccggg agtgcgtttt
     7681 tatatcaatg cagcggcaaa atatattctg gaaaaccagc aggagcagtc agaaaaaaca
     7741 ggaaacagct acccgactgt aggaagtacg tttggagaat ggtcagtatg ggatctgctg
     7801 cgcggcatgt atacaggggc agattatatc aacagcatac cggagaatta tttttctgac
     7861 tatttaaaac gtgtggaagc acatgtagaa aataagaaag gaaatctgca tgcggcaaag
     7921 tctaccgagt ggtcaagact gatcttgccg ctgactgcaa tgggatatga tatccgaagc
     7981 gtggcaggct atgattttat tgaaaaacta tctgattcat tcagtttttc gtatcgccag
     8041 ggcattaacg gtccgatctg ggagatcatt tccatgaaca gcggcggtta tgaatttgac
     8101 cagacggatc atccagaaac agcaaatacc tttggaaaaa tgctggatta cattctgaat
     8161 ctggagatca ccgatgcaaa cggaatcaaa ggcggctggg cattgtcagg aaatgtaccg
     8221 gatgccgata tcactggcat gacactgaca gcatttgcac catattatct gagtcaggaa
     8281 aaatatgagc aaacagatgc aacctatagt tatgatgagt ttgcaagtgc ggtagaacgc
     8341 ggtattctcg tgctggcaaa tatgcagaag ccgaacggcg gttttgagag ctggggaacg
     8401 gtcaacagcg aatcgaccgt atgggcgatg atgccgcttc tggaaatggg gatcgatccg
     8461 aaatcagata aggtaacgtt gccgcatatc ggaaaaacat gcagctttgt caaagaagga
     8521 gcgacacggg acggcgttta caccgacaat atggtagatg cacttctgac cttctgggca
     8581 gccggaagcg gaagcagtgc gtcgatcggc ggatttaaac atgtaacggc aggctacgac
     8641 ggaggtggcg gttccggcac aacggtcaat ccgatggcaa ccgaccaggc actttacgga
     8701 ctgatcgcat acgaccgttt tgcacacaaa cagaattccc tttttgatat gcaggatcag
     8761 ctggacggta gttatctgaa tgccacggca gatacttata cgatcaccta tgacggtcag
     8821 aaagagggcg agaccttcac aacagaagct tccccttatg cagaagtact cttatccgaa
     8881 ggaaaagtaa ccgatgaaca ggaagcattt ttgacggagt ggaataccaa accggacgga
     8941 agtgggatca gctattatcc gggagagctc ctttccatgc cggagcagga tatcacgctt
     9001 tacgcacagt acggacagcc aagttatgca ctgaaatttg aattaaacgg tggtactctt
     9061 tccgatgaca tcattcttcc ggacacctac agtccgaaag accagatcac actgccgacc
     9121 gcagatgaga tcacaaaagc aggatgtaaa tttgacggat ggtacaccaa tgcggaattt
     9181 accggaagca aagtgaccga aataccggca cacagttatg gcgataagac tttttatgca
     9241 aaatggacgg taaatactga aaaagcaaat cagttttatg cgatcgtgaa tcgtctgtcc
     9301 ggccatgcga cagccatttc tgataaagaa gacattgaaa aggcgagaga attgtatgat
     9361 tcaatgctgg atatcgagcg ggagcggatc acggcaagta cttaccatac tttcctgaag
     9421 aaggaaaaag aactgaagga actgcttgca agcatggatc aggcagaaca ggtcagtgcg
     9481 atgatcaaag cattggataa agaactgacg cttgcagatg agcagccggt agtgcgtgca
     9541 agaaatgcct acgatgcact gacagaaaca gaaaaagcga tggtagaaaa tctggatatt
     9601 ctgacgaaag cagaagaaaa gatctcacag atgaaggaga acaaagaaaa ggcagatgcc
     9661 gtgatccgtc agatcgaagc gatcggagat gtgaccttag attccagaga ggagatcaca
     9721 gcggcaagag aagcattcca ggcactgaca gagagccagc aggcacttgt tccggagcgt
     9781 gtgagaaaac ttctggagaa tgcagaaaag aagatcacag agcttttgga gaagaaagac
     9841 aggatcgatg cattttcaag ctgcgctaag cggatcccgg aaaaagtgtc acttacagat
     9901 gacagtcttt cacttcttat gaatgcccat gccgcatggc tgaaattgaa cgatgaagaa
     9961 agggcacagg tagatggaaa gctgatcgaa cagatcaaag aaaaagaaaa agaactgcag
    10021 gagcttgcac aggcagaggc aacggatgag gattatacgg cagtcaaaga gatggaagag
    10081 cggattgctg cggcaagcgg tgaggtgaaa cttgaggatg aagagaccct gaaaaagatc
    10141 cgaagtgatt ttgatgcgat gacaaagatc cggcaggcga tggttgtcaa ttattattct
    10201 ctcgttgcac aggaaatgga tctggagaag ctgaaaaaag ataccgccat cgcagaagcc
    10261 atcagcgagc gaattgcagc acttggcgag atcacctttg aaagtgaaga gaggctgacg
    10321 gcactgcgaa a
//
LOCUS       DAWDWH010000052        10219 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3339_length_10219_cov_18.112554, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000052 DAWDWH010000000
VERSION     DAWDWH010000052.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10219)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10219)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10219
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3339_length_10219_cov_18.112554"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3717..3726
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2744 a         2669 c         2002 g         2794 t
ORIGIN      
        1 tagaacatcc ttcgccacct ccggataaag ttccttcatc ttcttcatgt cccgctcatt
       61 ttgcatctca tccagatacg cctgcggata catgtttgcc ggataaacag gataaccacc
      121 tgccatgccc atccacggta ccatcgggct ttccaccggc atcatcgctc ccggcatcgg
      181 ataaccctga tgatatggca taaaattcca cggaaatttt tcttcaaaag aatccacgat
      241 tgtctcactc cactcgcaaa acatttgcta taaaatatgc tttcccctgt gcagacgtga
      301 aaaaagtggt acaataaatg atgccaagga tgaaaaagtc caacgtcgtt cttgcttgca
      361 agataagacg attgaccttt tcatccgctt aaacatgagg aagaagcaat ttacgcagta
      421 aatttgcgta ttccgaatgt ttacaggatt gcgaaagctg attttcagcg gagcaatccg
      481 tggcatcatc tttctcacca tgcagattta acatgaatgg atttttggag cttgctcaca
      541 aaagaccatt ctgataaagc aaaccgaggt gtccccacat gcgtacgatc tttcatattg
      601 atgtaaattc cgcttatcta agctggacgt ctgtcgaaaa tttaaaaaca ggaaacggtc
      661 cggatctgcg agaaatccct gccatcatcg gcggcgatca gaagacacgc cacggcgttg
      721 tcctcgccaa atccattccg gcaaaaaaat tcggcatcca cacaggcgaa cctgtcgtga
      781 atgccttaaa aaaatgccca aatcttgtaa tggcaccgcc caaccacgac ctttatcatg
      841 cgtacagctg ccgcatgatg gactttctgc acagcctgac tcctgatatt gaacaggtca
      901 gcattgatga atgtttcctg gattttaccg ggatttccca tcactatccg tcaccttacg
      961 ccgctgccag atacatcaag gacgaaatcc acagccgctt tggctttacc gtaaatgtcg
     1021 gtatttcttc caataaactt ctggcaaaaa tggcatcgga ttttgaaaag ccggataaaa
     1081 tacacactct gtttccggac gaaatcccgc agaaaatgtg gcctcttccg atcggagaac
     1141 tgttcatggc aggcaaatct tccgtgaaag ctctgaacaa cctcggcatc cgcaccatcg
     1201 gagaacttgc tgcctccgac ccgaaactga tcacgctcaa tctgaaaagc catggaacca
     1261 tgctctggaa ttacgcaaat ggcatcgata acagcccggt ctgttccacc ccggcggagg
     1321 caaaagggat tggaaactct accactctgt cctccgacct gacaaaagaa tgcgaggctg
     1381 taccgatcct gcactccctt gcagaaagtg ttgcatcccg cctgcgaaaa gcacacaaaa
     1441 aagccggcag catctgtgtg gaaatcaaat actacgattt tcagtctgtt tccagacaaa
     1501 cccagattga tattccaaca aattctaccg acctgatcac tcagacctct aaacggctct
     1561 tccacgaact ctggaatcag aagcccgttc gcctgttagg tatccgcact accaaactcg
     1621 ttgatgaaaa cgagcccgtc cagctttcct tgtttgacct ggatttttcc gatatttctg
     1681 caaacaaaac aaatgccaat gccgattttc cgaaaaaacc atccccggaa aaacagaaaa
     1741 aactggatac agcacttgat tctattcgca aaaaatatgg aaaaaaagcg gtgacacgag
     1801 caagtgagat ggagtaaaaa actacctctc cactttcttc tttcttctct tctgtggctt
     1861 tttcgtcttt ttcttcacag aatacccgaa cgtcccaatc tcttttccga gatccagata
     1921 ttctccgtgc gaatatgcaa tcagcccact tttattgagc tgaaattttc cattctcatc
     1981 aagatatttt tcatcgacat gaactgccag tacttctgcg aggaacatat ggtgcgaacc
     2041 gagttctttc ttctcggtga tacggcactc gatattgacc ggtgcttccg cgatcagcgg
     2101 tgctttgacc ttcgttgctt ccaccgcagt cagcccggtc tctttgaatt tatcgacatc
     2161 ccggccggaa cgtacgccgc agtagtcagt tgcccgtgcc agctcttttg ttgtcaggtt
     2221 gatgacaaat tctcctgtct catccagaat atcatacgaa taacgctgcg gtcttaccga
     2281 aatataaacc atcggcgggt tggtacagac ggttcccgtc cacgccaccg tgatgatgtt
     2341 tggtttctcg ccctccctct gacagctgac catcactgcc ggaagcggat agatcatatt
     2401 tcccggtttc cagatttgct ttgccataat tataattgcc tcctattgct gcattgccgt
     2461 cataaagctc gggatcagct gtttctttct ggaaacaaca cccggcagga tcacagactg
     2521 ctcgccttcc tgcggctctt tgtggaacgc ttcgcttacc acatttttgc tgtctccgcc
     2581 gtagaacagc agctctgtgc tttcgttgat aatatctgtc agcataaaga acagcatgga
     2641 ttctccgtgg cttcccagag ccttttccat atatggaagc agttctttct tgatctcggt
     2701 cagctcatcc gcatttacag aagtgatctg tccaacgcca aagctggatt tatttaactc
     2761 aaatttcttg aaatcctgat agaagatttc ttccggtgat ttattcttta agttgcttcc
     2821 cgctgcaaac atctcggatg ccagctcttc gcatttgaca ccggcgatct ccgccagctt
     2881 ttctgccgct gccttgtcca tcgccgtaca ggtcggagaa cggaacatca gcgtatccga
     2941 aagaatcgca gaacagagca gtccggcaat atttggttcg atcgtcagat ttttttcctg
     3001 ataaacctga tacagaatcg ttgccgtaca tccaaccggc tgattgcgga aataaaccgg
     3061 agccatcgtc tgcaaagatc cgatcctgtg gtgatcgatg atctccagaa tctctgcact
     3121 ttcgatgttg tcaaccgcct gcgaaatttc attgtgatcc acaaggcaga gctgtctgcg
     3181 tttgataccc agcaagttac gtctggaaac cattcccaca tattttccgt gtttattcag
     3241 aaccgggaaa tctctgtgac gcagttttgc catgacttct ttgatatcat ccgtataatc
     3301 gtcaatactg aatttcacca gattctcact ggtcataaaa tgtttcaccg gcatactctg
     3361 attgatcagt cgtgcaaccg tataagtatc atgcggagaa ctgatcacaa aacattctct
     3421 ctcctctgcc agtcggcgga tcgtcttcga aatcggaaca cccatacaga caatgataca
     3481 gcttgccccc atctcgatcg cacaaagctg agcttcataa cggtttccca gaatcaccag
     3541 atcattctgc tccacacttt gttccatcac ttccgggctg gatgccgcga tcagcacctt
     3601 tccatccgta aaatgtgcat gctcatttcc aacgatcatc gttccatcta ctgtttccag
     3661 aatgttgcga tacggcgttc ttgcctcaga aagtgctgca ttgttgtaaa catccannnn
     3721 nnnnnntcca tgtacgattc tgtaatgtcg tttgtcgtga tcagaccctc cagctcctga
     3781 ttctgattaa ggatcggaag ggtaaataca ttttcattcc gcatcagcgt ccatgctttt
     3841 ttcagagaaa ttttactgtc cactccaccg actttacgca catccatgtc tttgacacgc
     3901 gtaccgacat tcggcatata gcccggtgta ttcatctgaa aacgtttcag cacatacagc
     3961 gtttcttcgt tgatctgacc ggcacgcttt gcctcataaa cgggattttc ctccgttctg
     4021 tttttgatat ctgcataagc gatcgcagaa cagatcgagt ccgtatccgg attcttgtgt
     4081 ccgatcacat aaattttcct cttattgttt tccataatag cctccatatg attataacgt
     4141 aaaacgctcc ggcagtaact gctccagtgt ataacttttc cagttttccg tatcttttgc
     4201 cagcagaatc tcaaaatctg ctttgcagaa ttctgccatc acctgacggc acacgccgca
     4261 cggcggacag taatctccgg cttcgccccg tttatttccg acgatcgcta tcgccgtaaa
     4321 atcccgcctg ccttctgaca ctgccttaaa aaatgctgtc cgttccgcac agttgcccgg
     4381 cgaatatgcc gcattctcaa tgttgcatcc ctgatagatc ttaccgtctt ctgtgagcag
     4441 tgccgccccg accagaaact ctgaatacgg tgcataagcc tgctgctgtg ccagaaaagc
     4501 ctgtcttacc agctctgttc tgtctctgtc atccatcctg caaatccctc cgttattcag
     4561 gtgcgatttc aagtgccagt tccatcatat catgaaaact cttttcacgt tctgctgagt
     4621 ttgtcgcctc tccggtaaca atgctgtccg agatcgtaca gatcgcaaga gcctgttttc
     4681 cggttctcgc cgcattcata tacaatgccg ccgcttccat ttccacggcc agtacgccca
     4741 ttttccgcca gttcacatcc aggttggaat cgctgtaaaa tgtatccgaa gaaagcagat
     4801 ttccgaccat ataatgcaga ccttcccttt tttctgccgc ttccactgct tttctcaaaa
     4861 gtggaaaaga tgccgttgga gcaaagcttc ccggaagctg atactgtgcc gcaaaattcg
     4921 aatttgtgca ggcaccgatg ccaaatacca gatcccgcag atgcagatct tccctgagtg
     4981 cacctgccgt gccgatccgg ataatatttt ccacatcata accattgaac agctcataag
     5041 aataaatgcc cattgaaggc attcccatgc cgctcgccat cacagaaact ctcttgccct
     5101 tataagttcc cgtataacct gacatctggc gcaccgagtt gaccagaact gcatcttcca
     5161 gataatgttc cgctataaat ctcgcccgca gcggatcacc cggcatcagt actgtttttg
     5221 caaaatcacc cttttttgcc tctatatgag gtgtcggaat attcataagt ccatcctttc
     5281 cgcagacacc cgtctgctcc tgtttttatt attttagcat ttttgaatgt catggtcaaa
     5341 agttatttcc caagttgctt tacaaattca tcgcaaaaag gtatattgga atcatatttt
     5401 acagacccgg cagcaagtct tccggtctgc atctttttgc cttaacacca caaaatcaac
     5461 aggaggtatt acaatgtcag aagaattaaa aacatctgaa gaactcgcag cagaagccga
     5521 aacttcggct gctgaaacac cggaaaccac cgaaactgcc tcaccggaag aaccggcaga
     5581 aaccatggct gattacgctg aagaactgga agcttctttc aaacagatcc atgaaggcga
     5641 tatcctcacc ggaaccgtaa tcggtgtttc cgaatctgag atcacactgg atctgaaata
     5701 ctacaccgaa ggtatcatcc gtctggaaga tttcacaagc gatcctggat ttaatctcaa
     5761 agaagatgtt cacatcggtg atgagatctc cgcaaccgtg atccgcagag atgacggaga
     5821 aggccacatt ctgctctcct ccaaagaagc gaatgacatt ctcgcatggg acaaattcaa
     5881 aaaatacatg gaagaaggca ccaacctgac cgttaaggtc ggtggcatcg taaaatccgg
     5941 tgtggttgca tacctggacg gcatccgcgg tttcatcccg gcatccaagc tttccctgaa
     6001 ctatgtagaa gaagacgctc tgaacgactg gctcggcaaa gaactggaag tccgtgtcat
     6061 caatgctgat gctgaaaaca aacgtctcgt cctttccgca agagagatcc tgcgtgagaa
     6121 agcagatgaa gaacgcaaag caaaagtttc caacgttcag gtcggactcg ttaccgaagg
     6181 aaccgtagag accatcaaac cttacggtgc atttatcaac cttggaaatg gcctgtccgg
     6241 tctgctgcac gtttctcaga tctgcgaaaa acgtatcaaa acaccggcag ctgttctgtc
     6301 cgaaggtgat actgtaaaag tcaagatcac tgcgatcaaa gacggaaaac tgagtctgag
     6361 catgaaagcc ctgaacgatg tagccgcaga agaagtcgaa gaagagactt tcgaacttcc
     6421 ggagacagaa gccgctacca caagtcttgg ctccctgttc aaaaacatta agctttgatg
     6481 ttacgatgaa aagatgcata cgaattgttc cgtactttgt actccaacaa ttccgtcatg
     6541 tattagtaca ttccaacaaa aaacaggtct gttcaagacc tgtttttgtt attttcacac
     6601 acttttcaca gagtgttcat attctattca tacatcattg ttactatgta aacagttcaa
     6661 gagtgaaagc ttttgataca ctttacacaa agcaataaat tttttcataa tagcctccaa
     6721 tgtaccagac attgggggca ctcctcattt tacagatatt tttgtgccgg atttcacaca
     6781 ctctccgatt cgtttctatt tttcaatccc cattctggat gcctgaccct catcaaacgg
     6841 atgcttgatc tttttgatct gcgatacata gtctgccgca gcgatcatcg cctcgcttgg
     6901 tgtatttccg gtcaggatca cttccagctt gtccggtttc tttttcagaa agtcaagtac
     6961 cagcttttca tccagcagtc ccgcactgat cgtatagatc gtctcatcca gaaaaagcac
     7021 atcgaagtct tcctccaccg ctttctttgt caccttctca aactgactgt tatagaaagc
     7081 aagacgttct ttcttttcct gttccgtcat ctgaaagctg aatttctcct gatccagtcc
     7141 gtttacgatt gaaacattct ctatcttttc cagaatgttt cgttcactcg tcttgttgtt
     7201 tttcataaac tgatagatca gtacacggta gccataaccg gctgcccgca cacagagtcc
     7261 cattcctgtt gttgtctttc cttttccgtc cccgaaataa atatggacaa gccctgttcc
     7321 ttttcgattc ataatttctt tttacacctc ctgcatattt gatcaaatca gatgcatagg
     7381 aaatctgata catgattctg tcggaatcac tttgcaggag gcttaagctc actcctacag
     7441 ttccagtttc atatcgccgt ctttgatcca gccttttttt ctcataaatt cggacactgc
     7501 cagtgtgatc actgccggca gcacaatctg gaataccaca atgatgatca ttgtcatggt
     7561 acctccgatt tccggtgcca tcgtctgata ggtcatgatc tgccctacaa atcctgcggt
     7621 tcccataccg gagcctgttg cattgctcgt cattttgacc agcattgtgc tgaccggtcc
     7681 caaaacggca ctggaaacaa tcgccggaat ccatatgatt ggttttctta caatattcgg
     7741 cacctgcagc atagatgttc caatgccctg tgccagaagt ccggcaacct tattttcacg
     7801 atagcttgcc accgcaaatc ctaccatgtt acagcaacat ccaatcgtcg ccgcaccggc
     7861 tgcaagtccg gaaagattta aaataatacc aagagccgca gaactgatcg gcagtgtcag
     7921 gatcattccc atcagcacgg aaaccacgat tcccatgata aatggctgct gttctgttcc
     7981 ccagttgatg attgcaccaa gtccggtcat aaatttggaa atcggcggtc ctacgatcag
     8041 accgactgca catccggtta cgatcgtcac gatcggcgtt accagaatat ctacttttgt
     8101 cttaccggaa accagatgtc ccagttcaat tcctgcaaat gccgctacaa atgcaccgag
     8161 cggttctcca ggtcctgcca gaacaacgct tccgtcaacc agaagtgaac ctgccagaat
     8221 cttacttgca aatcctcctg tcatgccggc cgttgccgca gaaagcacca ccagcggact
     8281 ttcctgataa cggtgtgcca caccgacacc gataccagca ccggtcatcg ccgctgccat
     8341 ctttcccatg ataaaaagca tgtctccaat gtttccgccg atcagatttc caatctgttg
     8401 gattattgta ccaacaatca gtgtagaaaa caagcccaaa gccatgccgc tcaaaccatc
     8461 aataaaaatg tggttcagta attttttcat gcctttcttt cctcctcgat aaagttttcc
     8521 cggtcagact gcctgtatgc acttcccgga gtgagtttac acctttatat tgattcttca
     8581 acaatgtcat ttcaggtgtc ttgtcacctt gagaatgtag catattttcc agcatttatc
     8641 aagaatattt ttctgtctga actctttaca gaacgaaaag tttattgtat gattcagaat
     8701 gatgtcagag cattggcaga cattgttttg tatcttgatc catcaaactg tttttcagga
     8761 gaaaggatgt agcatgacct ctaagaaact gaaaaaaact tttactgctg cctttttgtc
     8821 cggcagtatt cttttacaga attttcctgc tttggcagct gcttctgata caacagactg
     8881 gctgaaggaa tcgccgttcc gccaggatat aacaaccgaa aaaagtactc cgacagaatc
     8941 cgttccatcc gaaaccgaaa cggtatcccc tgaaacatta agcacttttc agaccgaagc
     9001 ggagacagaa gctgaaacgg aaatcccgga agaaagcaag tcttccaata acacagcttc
     9061 ttttcttatg gctgataatt ccgatacctt ttcacagatt ctgcaggaag ccactcttac
     9121 accttcctac acttttgatt ctctgaatca ggatttttcc tggaaagagc tgaaaacaga
     9181 gcttaccaga cgcgtcaaca gctattcttc caagtggtct gtttatctga aagacctgac
     9241 caccggaaat gtgataagca tcaacgaacg tgcccaggag tccgcaagcc tgatcaaact
     9301 ttatgtgatg ggagccgtaa tgcaggaaat ccagaacggc aatctggaaa tgaatgacac
     9361 gaccaaacat cttctcgatg aaatgatcac agtcagcgat aattctgcca caaatgagct
     9421 ggtccgttat ctgaacaaag atcacgacca caaaaaaggc atgaagaaag taaatgcttt
     9481 cataaaagca cagggatttg aatatacgca cgaatacaac ggtctggaag acaccagtct
     9541 gtggtacgac gctgatacca aaaacacaac ttccgccaaa gactgcggtc gccttctgga
     9601 acgtatttac cgcggagaat ttatctctca tctcgcttcc cgccagatgg aagaacttct
     9661 gttaaatcag caggttacct acaaaatccc atccacgatc ccgtcggaat cgcgtgttgc
     9721 aaacaaaacc ggtgagactt ccgactgcga aaacgatgca gcgatcgtct acacaccaaa
     9781 aggcgattat attttatgta tcatgtcctg cgaggtttcc agcaaaagtt ctgccgtaga
     9841 ttctcttcag gaaatgtcct ccctgatcta cagctacttt atgaaccgtg acacctcaaa
     9901 agtcagccgc caggtggaag ctactgcaaa ggaaaccgat taggtttgat atacaaaatg
     9961 taaaattaat aaattttatg ttgtatgttg tcaattcatt ttatatatgc tagactaata
    10021 gtaatcagat tagagctaac gtaggaatag tctggtggtt gttccttccg gaaacggaaa
    10081 ggagatattg tatgaataca cttgaagtat taacacttgt gcttgtaatt tttgcagtac
    10141 ttacatatat tgacaaccac cgtaattcaa gtcgaacgca cgtgagactt ggtgcgtgat
    10201 tctggtcagc gaaagctga
//
LOCUS       DAWDWH010000053        10158 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3357_length_10158_cov_13.895081, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000053 DAWDWH010000000
VERSION     DAWDWH010000053.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10158)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10158
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3357_length_10158_cov_13.895081"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4555..4564
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3047 a         1979 c         2533 g         2589 t
ORIGIN      
        1 agcagttatt tggttatgag caagtagcaa agcggattgc gaatatccgc ttgacgaata
       61 tctctgcaat tcgccggagg ctgtatcaga cagagtcagg tcacttcata tcggatgtaa
      121 agtacgaaaa atcatgcaga ttcaatttca taagtaaatc cggcatcggt gatcgttttg
      181 atggtatcct ggatgccctg accgccggca gtaagatatc cggaagcaaa gatagaatcc
      241 acgacactga gcagtttctt ttcctctcct ttgaagtaga cttctctgcc ggcagcacag
      301 cggatgtcgg acttcggtac gagcagtctt gcaaggcaga ggactttcat gcaatattcc
      361 gttgtcagac cggaaatatc tgcatcccca agcggtgtgc ccttgatcgg cagcagaaaa
      421 ttgattggca gagcctccgg ctggatctca cgcagctcca gaagcatatc cacgacgtcc
      481 tctttgcttt cacccatacc gatgataccg ccgctgcaga tttcaaatcc cagcccctga
      541 agcatgcgga tgtttgccac acgctgctca aacgtatgag ttgtgcagat gttgtgatag
      601 tacgcacggc tgctgttcag attgtggttg atgcggtcaa gaccggcatc cttgagcatt
      661 ttcgcctgct tttccgtcag aaatccgata gaacagcaca ggtgtgttcc ctgttctttc
      721 attttacgga tgcggcgggc gagctcttca atctcggcat cggtaaattt catgccgcta
      781 aggccaatgc agtgtctgga taaatggtgt tcatttacaa aatcattgtc gtgatagagc
      841 ttttcctcat ctacccactt gtatttctca atatccgctt tggaacggca ggactgggca
      901 cagtaggcac aatcctggga acagttgccg ctgcgggcat ttgtcagaat gtgaatgctg
      961 acgcggtttc ctttgtattt ccggcgaagt ccctccgcac gggcgatcag atcatcgagt
     1021 ttctcatccg gtgtatccag gatggcgatg gcatcctcac gggagaggat cggatcatcc
     1081 tgatcataaa aatttgggtt catgatgtta ccttccttgt aaaattctgg taacaatgta
     1141 acatgagaaa aaaagattgt caacctgaaa atcaagaagg ttgacaatta aaaaacaaaa
     1201 cttgaaagat tcttttcata tgccacttgc atgagtgtgg gtaaaatgtt atcttaatcg
     1261 gagaagactt caacgtctgc tgacaagtgc ggagcttcgc tcagaacatc cgctggaaga
     1321 gcagataaaa aacagtgtaa aaaagaaaat ctctgtggaa taaaaatgtt ctgcggagat
     1381 tttttatgtt tgcaaagctt acgatgcaat gctatcataa aagagacagg agaggcattc
     1441 ttgaacatgc aatgcatagc atacatcttg cagcaagaat accggaagaa tgtttcaaca
     1501 aaacagcagg agataaaaca aatggaatta tgggatatat acgataaaaa taaaaaaccg
     1561 accggacgca cgatgaaacg aaacgactgg cacttaaaag acggagaata tcatctgaca
     1621 gtgcttggcg ttgtggcaag agaagacggc acttttctga tcacaaagcg tgtgatgaca
     1681 aaagcatggg cacctggctg gtgggaagta tccggaggtg cagcacaggc cggagaagct
     1741 tcttacgatg ccgttctgcg tgaagtaaaa gaagagaccg ggcttgatgt aagcaatgcc
     1801 cagggcggtt atctgttcac ttataaaaga gaaaacccgg gcgaagggga caattatttt
     1861 gtggatgtct atcgttttgt gatggatatt tcagatgaag atctgaaact tcaaacagaa
     1921 gagacggatg gatatatgtt tgcgacgaaa gagcagatcg aagcatttgc ggcagaagga
     1981 aaattcctgc attatgacag tattaagcag gcttttgaat tttgacagcc gaccttgcag
     2041 aatacagaca ggctgatttg gatccttata atgaatgcag aatgatggat gcagaaagga
     2101 gaaaaaatga aaaagcgaat gaaaaagatt atagtacttt tgatggcagc ggcactggcg
     2161 gttccgggac agagtctggc gggtgcagca aagatggctg tcgtaaatgc acaggagcag
     2221 gagacgaaag cagttgagct tcagagtgaa tatttcaata tggcaagcag ttatgagaag
     2281 gttgtgtaca gcaaagcaga tcatggcttt tatctggtaa aaaaaggtga tcctttttat
     2341 tcaaatcagc agaaatttga agtttctttt tatgatattg acagcaattc ttatgagcag
     2401 aaaacgacca ttttacgggc agaagacagt tttgtgaatg acgaaggaat ttattatata
     2461 aaaacagaaa acaaaaggct ggcaaatgcc attgaacaaa atgggaaaac gtatacgtat
     2521 gactgcaatg ccacgctgta tgaatatgtt tttgaaacag gggaaacaaa agaaatcacg
     2581 ctggacagca tgaaaagcac cgcgaatttc tcagggttta tcaatgcact tggcgtagat
     2641 aaaaagggaa ggatctatgt ggccaatgcc agcgatgatc tttgcctgta cgacagcacc
     2701 ggaaaatctc tggcaacagc accgtatgaa ggaagcattt tacagtttta cggatttgat
     2761 acggtcagcg gcaattttta ttacagagga acttataact ggagatactg gggatatgat
     2821 catgacatgg cgtctttaat ggcaggaaat gtcggtgagg acaatacact tcatctgccg
     2881 gaggcaaatc tgatgatgct gtatcagtct tatttctatg agagaaataa accggcagag
     2941 atgttaaatg ataaatatct ggcagtgctc aataccttcc agagtccgaa cgccattacg
     3001 attctggatt ccaacagcta tgattacaac gatcacacgg agcagagtac ggtgatcaat
     3061 atcattgaca gcagtgtttc ggtttcttct ctgaatattg cagacaaaga tgcgatcaaa
     3121 tttgtggcac ttgcgggaaa atcaacctac aaaaataatt ttgatgtcag cagttacgga
     3181 accagatgtg ctttgagtga agatgaaaag agcatttatg tcaagacaga tacaaacacg
     3241 ctcacgcagt actccatgga agatggaaag aaaaaagtgg acatcgttac cgagcatccg
     3301 gtctacagtt tctgggtgga tggtgatata tgccgtgttg tagaaaaaga agaggatcag
     3361 ttttatcttg aaacattgga tctgaaaatc ccgacaactt ttgaagtgca ggcaccagag
     3421 tccatgaagg tatctgagag cgacaagctg atctgtacaa cagaaggttc ctgcaaaatg
     3481 gattacactt atgaatcctc ggatccgaac atagtaagtg tggatgaaac aggaggtctc
     3541 aatgcctgga aagcaggaac agcgaccatc atgatcacag cagaaacgat aggcgttaca
     3601 aaacaggtca cgatcacggt ggatggttca cagtacgaag atgcagaagt gttttcaaag
     3661 gtaaacctgg agggagcagc atccgacaac attcatctgc cgcattattc ttcttattat
     3721 ggaagtacca cgaagtctta tctggcacag acagcagacg gcggatatca gcgtgcagaa
     3781 tatatcaatg ataagatcgt ggttgagact tatgacagca aactgaatct ggtttcttct
     3841 aaaaatgttg cggcagaact tccgattttt ggtgggatct atattggcga aaattataat
     3901 tatgcagttt ttggacagat gaacaaagaa gaatcggatg agtgtgaagt attccgtttc
     3961 gtgaaatatg ataaaaactg gaatcgcctt gcacagtgca gtgttaaagg agccaacacc
     4021 tacattccat ttgatgctgg cggtctcagc atgacggaaa cagacggcaa actttatatc
     4081 cacacctgcc acgaaatgta tcagtcggac gacggatatc atcatcaggc aaactgcagt
     4141 tttgtggtca atgaagaaga tatgacactt gtggattctt atacaggagt catgaatctg
     4201 ggagaaggtt atgtcagcca ttctttcatg cagctgatca gaacggacgg agaatacatt
     4261 taccgtgtgg atctcggaga tgcttatcca agaggcattg catttaccat gaccaagacc
     4321 ggtgacaaac tgcaagatcc gagtctgtac ggcagtcttt tcacaatcga tggaaataca
     4381 ggctataatt atactggata ttctctgact ggtctggaac tgtcagaaga atattatctg
     4441 gttgcaggcc ttggtgtcga aaaactgaat gatgatcaga aaaatatatt tataagcagt
     4501 tcgagaaaag cggaaccttc cggaaacaga acctggatca ccaactatgc aaatnnnnnn
     4561 nnnntgcaga cgttgatgtt accacgccaa aactggttaa gatcacgaaa aatcagttcc
     4621 tgctgatgtg ggaagaaaaa gcatccggaa aacagaatta caccacaaaa atgatgctga
     4681 tgaatgcaga cggaagcagg acaagtaagg tatattccgc accgcttgcc ctgtcagaat
     4741 gtcagccgat ctgtgataca gacggaaacg tcgtatggta tgtcacagac aacggcaaac
     4801 cggtgatgat caagatcaat ccatactgcc ttgaagaggt aagcaaagaa accggcggaa
     4861 gtctggaaga gaataccggc ggaaacaatg gtggaaatac cggagataat aacggcggaa
     4921 atactggagg caataccggc ggaaacaccg gaggtaatat ttcctggggt gatggtgata
     4981 tcgactggga ttggggattt gatccaaaac cggacaattc agacacagat aaaacagaga
     5041 atactcagac aaagccgcag cagacgataa aagtcggcaa gccaaagatt agcttaaaga
     5101 gatctaaaac gaagattcag gttcagttta aaaaagtaaa aaatgcgaag aaatatcaga
     5161 ttcagtatgc gaccaacaag aaatttaaaa aagcaaaatc caaaacgacc acaaaactga
     5221 aatatacgat caaaggatta aagaaaaata agaaatacta tgttcgtgtg cgtggtgtaa
     5281 acggaacaac aaaaggaaga tggtccgcag taaaaaagaa atgacaaagt aatatgagca
     5341 gtaaagaagg aaggtttttg ttttgaaaaa cagtaaatat gaaattgata tgtgcaatgg
     5401 aagtatcatg gacaaattaa tttcgttttc actcccgctg atgttatccg gtattctgca
     5461 gctgatgttc aatgcagtgg acatcatcgt agtgggaaga ttcagtggga aacaggcatt
     5521 ggcggcggtt ggttcaacca ccgccctgat caatgttttt accaatcttt ttatcggtat
     5581 ttcgcttggg gcaaatgtac tggcggcaag gttttatgcg atgggcaaat caaaagaaat
     5641 gtcggaaaca gtacatactt cgatcacgct ggcgatggtc agcggtgtga tcatggcatt
     5701 tgtcggtgtt ctgtttgcga gaggtgcact ggaattgatg ggaacgccgg atgatgtcat
     5761 tgatcagtct acgctgtata tgcggattta ttttatgggc atgccgtttt ttatgctcta
     5821 taactacggg gcggcgatcc ttcgagcaat cggagatacg aagcggccgc ttctttttct
     5881 gatcatctcc ggactgatca acgcagcatt gaatatgctg ctcgttattt actgtcacat
     5941 ggcagtggaa ggcgttgcga tcgctacggt gatctcacag ctgatctcct gcatcctggt
     6001 attatggtgc ttgtataaat cggaaggcag ctaccagctc cgtttttcaa aactgcagat
     6061 caaaggcgtt tatctgaagc agatttttca ggtcggaatc ccggcaggga tccagagcat
     6121 gatcatcaat ttttctaacg tacttttgca gtcatcggtc aattcctttg gttctaccgc
     6181 catggcaggt tatacggcgg ccaataatat actgggattt ctctatgcgg cagtcaattc
     6241 gatcacgcag gcatgcatga gtttcacaag ccagaattat ggtgtcggca aatacaaacg
     6301 tatggataaa gtgttgatcg actgcctgat cctgacggtt gttgttgccg gagtgcttgg
     6361 atgcggttct tatcttctcg gaccggagat tcttcggatc tacacagaag aagagaaagt
     6421 tatccgctgc ggaatggaaa ttttatcgat cacgactgtg ccgtattttt tgtgcggtat
     6481 catggatctg tttccgggag cactcagagg catgggctat tctgccgtac cgatgattct
     6541 ttcgattatc ggtaccgtag gtatgcgagt ggtatggatt tttggaatct ttccgcatta
     6601 tcgttctttg tatgtgctgt ttatttcgta tccgggttcc tggctgttca cgatcattat
     6661 gcaggtgatc tgcttttatt ttgtaaggaa aaagatacat cagatgtgag aaaactccca
     6721 tctgatatag cctccggcag attgcagagg tgcacagaag aatatatcat gcgatccatg
     6781 acgtgcaccc cgcagcaaga atatcggagc cattcttgat aaaggagacc gcaaaattgt
     6841 cagatatcat agaaattacg gattttgaag atccgaaact ggacgtatat gcaagaataa
     6901 aagaaatcca gcttttaaat cgtgcaaatc cagaagacgg gatttttatt gcagaaagcc
     6961 cgaaagtagt ggagcgggca ctggatgcgg ggtacgagcc gatttctatt ctggtggaaa
     7021 agaaacacgt cgaaggagaa gcaaaacaga tcataaaacg ttgcgggcag atcccggtgt
     7081 atacggcaga atttgatgtg ctcacaaaga tcaccggttt taaactgacg cgtggcatgc
     7141 tctgtgcgat gcatcgccgg aaacttccgt ccatgaggga aatctgtgaa aatgcaaaac
     7201 gcattgcggt gctggaagaa gtggtcaatc cgactaatat cggggcgatt tttcgctcag
     7261 cggcagcaat gcacatggat gcggttctgc tgacaccggc atgcagcaat ccactttacc
     7321 ggcgggcgat ccgcgtcagt atggggacag ttttccagat tccgtggact tttgtggata
     7381 actccgtttc ctggccggaa ggagggattc agcttttaca caaaatggga tttaaaatgg
     7441 cggcacttgc cttaagcggt gattcgatcc gcatcgatga tccgattttg cagaaagaag
     7501 aaaagcttgc tatcctcctt ggaacagaag gagacggtct gacatcccgg acgatcgcag
     7561 actgcgatta caccgtcaaa ataccaatgt cgcatggcgt ggattcttta aatgtggcag
     7621 ctgcaagtgc cgtagcattc tggcagctga gaaaagaaaa gtaagaaatg ggaaaacaga
     7681 ggaaaacaaa aaatatagat ctaacggtcg gaaacatcac ctcgagcctg tggaaatttg
     7741 cggttccgct gatgcttgga aatgtgcttc agcagctcta caacctggtc gatacctggg
     7801 tggtcggcca ttatatcgga gataatgcac tggcagccgt cggttcgtct tacacgttaa
     7861 tgaccttcct gacatcgatc gtcatcgggc tttgtcttgg aagcagtgca tttttatcga
     7921 tggcttatgg caaaaaaaat cagaatctga tccgaaacgg catttttatt tctgcggttg
     7981 tgatcggttc actggcggtg attctgaccg ggatcattta tatctggatg aatccgatga
     8041 tccgtctgct tcaggtgccg caggaaacga cagcggcgat gcgggaatat cttttttatg
     8101 tctttatcgg attttttgcc acttttttat ataattatgt tgcaaacgta ctgcgtggaa
     8161 tcggaaattc cgtcacaccg ctttttttcc ttggaatttc cgtgatctta aatatttttc
     8221 tggatttata ctttgtgctg gttctgcaca tggggatcaa aggtgcggcc gttgcgaccg
     8281 tgatcgcaca gtatgtttcc ggagtcggta ttctgctgta ttttctggtg cgttatccgg
     8341 aataccatat cagcagaaaa gatatgaaat ggaaccgtga aaacttaaaa cagatcatgt
     8401 cactttccgg ttttacctgt ctgcagcagt ccgtgatgaa tttcggtatt ctggtcgtgc
     8461 agggaattgt caacagcttc ggagcgaccg ttatggcagc atttgccgtg gcagtcaaga
     8521 tcgacacgat cgcatatatg ccggtcggtg atttcggaaa tgcattttcc gtctttgtag
     8581 cccagaacta cggagccgga aagaaagaac gtatccgtca ggggatccgc cagtcagtga
     8641 tcagcgtgat cctgttctgc attgtgatca gctgcggtgt ctttgttctg gcacagccgc
     8701 tgatgcagct gtttgtttcc gagacgagca gggagattat tgcagtcggc gtgcagtatt
     8761 taagaatcga aggagcctgt tatatcggaa ttggcatttt gtttatgctc tatggctatt
     8821 accgtgctgt gaaccgcccg aacatgtcgg tggtgctcac ggtcatttcc cttggaacaa
     8881 gagtgctgct gtcttacacc ctctccaaaa tctcgtggct tggtgtgaca ggaatctggg
     8941 tatccattcc gatcggatgg ttccttgcgg atgcggtggg gattctggtg tatctgcgta
     9001 gcaggagatt ggatgagaaa ggatgatatt atggaattag gggcatattt taaaagtatt
     9061 atcgatcagg atcgtgcagc tgttgtgatc tgcagcctgg atcacaaaat catttatatg
     9121 aatccggcgg cagctgccaa ttatcataag tatggcggag cagaactgct tggcaaaagc
     9181 cttctggact gccacggggc acattccaac gaaagaatca aagaagtcgt tgcatggttt
     9241 gccaaaagcg aagaaaacaa tctcatatat acatctcata atgaaaaaca gaacaaagat
     9301 gtatatatgg tggcacttcg tgatgcagat aaaaaactga tcggatatta tgagaaacat
     9361 gaatatcgca ataaagaaaa catggaaaag tatcatatga cttcaaagtc agaagtctga
     9421 aaatgcagga attgcaggca gaatatatca aatgtaagat gacttccgcc tccgtagacg
     9481 gcgagtaaca agtttgtatc agtggcggga ttcaatcgcc tatctgattt gatataagta
     9541 ttggtggatt gcgaatatcc gcttggcaca aatgaaaaga ggaatatatg gcagatcata
     9601 aaaataaaaa acagaacgaa acagtcgatg ccggactgtg tggaaacgaa aaaatagaag
     9661 aagccatcgc ggcacttcag caggaaccga cacaggaaat gctggcacat accctgacgg
     9721 taatccgcag gcgtatgcag gaaaacggac agtttatcgt ggcagtagaa ccgccgaccg
     9781 gagacggtca gatccgggtg caggcgataa agaccgctga cggaaaagcg tggtgggcag
     9841 catttacaag ctttgaagaa gaattaaaag gcggcggaag cgtaaaatcc accttcctgg
     9901 caaatatcga taaattgttt gcatccgctc tgaccgtacc ggaaatcgca ggagtcatca
     9961 tcaatccatg gaaccggacg atccagctgg ataaaaacct gatccagatc atagaaggtg
    10021 cgaaaagata accggatgac aagattaaga gctgcagaat gggtagtgta aaatagtttg
    10081 tattttttat ggcagttgca aaagaacaaa ttcgacagat catctcagaa aacaacatta
    10141 atagcgttgc agatatat
//
LOCUS       DAWDWH010000054        10105 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3382_length_10105_cov_12.191542, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000054 DAWDWH010000000
VERSION     DAWDWH010000054.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10105)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10105)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10105
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3382_length_10105_cov_12.191542"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5568..5582
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7014..7023
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8525..8534
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9272..9281
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2416 a         2504 c         1977 g         3163 t
ORIGIN      
        1 gagattgttt tcgaatcatc atggcgttat atattttata aaatcatttc tatggtgttc
       61 atttgcacat ttacacaaac ctccaccaca attttttatt tatctgcatc ctctgatgca
      121 tctttgcttt cttctatttt atcttccagc tctgccactt cttttgcttc tacggcagct
      181 tcttcacctt cagaagtctc tgcatctttt ttttcttctt ccggtactgc attatagtct
      241 tctttgattg ctttatagct cttggtgact cccatgatca ccatccctgc accgatcaca
      301 gcaaacacta ccataaaaat ggacatcagt attttatcgg ttccgctgct ggtcgggata
      361 tctttataca tatagtaggc ctgtgccagc agatatacgc ctgccatggc atagatcgcc
      421 atacgtcctt tatactttgc tctgtcattc ataatattat gctcctctct ttttgcctgt
      481 atttcacata ttatgatgtc tggtcaaaag atatttgccc tctttgatac tggattgttc
      541 cgtaggcatc atagttggag acttttgaca cccacatcat aatatcacat ttccaacaga
      601 aaagatagat aatacttatc cgtttttcta ttttcagttc aaatacaggc gaacgttttc
      661 tccgatcaag agaaaaaaag agccgaacaa tatgtccggc ccttgagatt ctgtaaaaaa
      721 ttatttacga accaggtatt ttctcatatc gatagagcaa gctaccagga tgatgagacc
      781 tttgatgatg tactgcatac tctggttgat tgagaggaac tgcagtgcaa cgaagataat
      841 tctcaggatg aatacaccaa gaacgatacc actgatctta ccagtaccac ctacgaaaga
      901 tacaccaccg ataacgcagg ctgcgatagc atcacactcg tagttcagac ctgtgttggc
      961 ctggttggaa ccgatacgtg ctgactcgat gaaacctgta ataccataca tgcatccagc
     1021 cagagtatgt actgcgattg ttgtccagaa tacacttaca ccagatacgt ttgctgcttc
     1081 cgggttagaa cctacagcga acatattttt accaaatgtt gttttgttcc acatgaacca
     1141 catgaaagca atcatgataa ttgcatacca aacatagttc gggattggaa cgccaaagaa
     1201 tttaactaaa cttcctgtta cgaagttttt gaaggaattg tccagacctg acagaggctg
     1261 tccattgttt ccgttgatca tgatgtacag aagcagtaca ccgtatgtaa tcagctgagt
     1321 tgccagtgtt acgatgaatg gatgcagttt aaactgtgct acgaaccatc cattgacgaa
     1381 acctacgata ccaccaacaa cgacaacgat caggattact accgggattg gcagtggctg
     1441 aagattcggg aacatctttg ttgcatagtc catagactgc agtaaagatg cggaaataca
     1501 tgctgtcaga cctacaactc gaccagcaga aaggtcggta ccggtcagta cgatacatcc
     1561 tgcgatacca agtgcgatcg gaatatttgc agctgacaga gaaataatat ttacgataga
     1621 agatacactc aggaattttt ggttctgtgt gtatgtatag attgcagcaa tgatgatcaa
     1681 gatatacatc aggttgttga tgaatccctc tttccagaag gttttcttgt ctttgctgtc
     1741 cagtgtttta taatttgcga tacttgcttt taaatttgaa aatgctctca cttacttcag
     1801 cctccttata catattttgc cgcaagggtc atgatctctt cctggttcgt ctcgctgaca
     1861 tttacttcac ctgcaagacg tccaccggac ataacgagaa ttcggtcaca gattcccaga
     1921 agttctggca tttcagaaga aactaccata actactttgt tcttatttgc aagatccaga
     1981 atcaactggt aaatctcgta tttagcacca acgtcgatac cacgtgtcgg ctcatccagc
     2041 agcagtactt ccggattggt cagcagccag cgtccaagga taaccttctg ctggttacca
     2101 cctgacagtg aacggatttt tgtttcctgt gtcggtgttt tggtatgcat cgcatcgatg
     2161 gaccactgag tatcctctct catggacttc tcgctcagat acagaccttt ctttttatgt
     2221 ttcttaaggc tggagataac cgtattttca cgaatactga gaattccgaa aataccggtt
     2281 gcacgacgtt cctctgtcag aagggcgaaa ccgttcttga tcgattcacg tgcgttgcgg
     2341 tttgtgcaaa ccttaccgtc cagtttgatc gttcctgatt ttcttgttgc aataccgaag
     2401 atgttctcca gtgtttcggt acgtccgctt ccgtccagac cggccagacc aacaatctca
     2461 ccacggcgaa ctttgaagga aacatcacgc agcagagagt actgtgcagt caggttttct
     2521 acttccagag caacatcgcc cggtttgttt gtctttggtg ggaactggtt tgtcagttca
     2581 cgacctacca tcagtctgat gatctcatcc attgtcattt cttttgccgg tttggtagct
     2641 acccatgtac cgtctcgcat gactgtgatg tcatcagaaa ttctgaggat ctctgccatt
     2701 ttatgggaga tgtagatgat accacatccc tgatctctca gcatatttat aattttgaac
     2761 aagtgctcta cctcttcttc cgtcagggaa gatgttggct cgtcgaacac gataattttc
     2821 gaattaaagg aaactgcttt tgcaatttct gccatctgac gctgtgatac cggcatagtg
     2881 ctcatgatcc tcttcggatc aacatcaatc tccagtttgt cgaaaagtgc ttttgtatca
     2941 tcatacattt tcttttcgtc taccatcgga ccttttttgg gataacgtcc cagccagata
     3001 ttgtccataa cactacgttt cagggcctgg ttcaactcct gatgaaccat tgctacacca
     3061 ttttccaaag cttccttgga gtttttgaaa ttgatttctt tcccttccag agaaatggat
     3121 ccgccgtctt ttacatacat cccaaacagg cacttcatca atgtagattt cccagcgccg
     3181 ttttctccca tcagtgcgtg aaccgttcct cttcttacgg tcaaagaaac attatccaac
     3241 gccttaacgc ctgggaactc cttgctgata tttttcattt caagcaaaac atcatgctcc
     3301 atatcctgac ctcctgtctt tctactactt acctttatac ttaaggaacg gccgccgacc
     3361 agcggcagcc gttacccttg tatactattt cacgaatgaa tttccatcat tcattttccg
     3421 ttctgtttca gaatcagatc ttattcttct cctgtgtaca tagcgtatgg gatgtagatc
     3481 ttattcttaa cgttttcaga gatgttgtaa gaatctgtgt tagccattac atcctgacct
     3541 gaaccagcgt tctttgtcag atctgcgata caatctgcca taccttcagc atcctgtttg
     3601 attgtacctg tcatcttacc gtctttgatc agctgtttag ctgcatctgt agcgtctaca
     3661 ccgaatacag ggattgtctt gcagtcatct gtacccaggt tgtatccttt gtcattcaga
     3721 gcagagatag caccttcagc cataccatcg ttgttgcaga taacaagttc gatcatgttc
     3781 ttgttttctt cgttgtactg tgtcaggttg gttgtcatgt agttgtttgc agctgttgca
     3841 ctccagttac catcctggtc aacctggtag cagtctgtgt tggaagaatc aaagtattcc
     3901 agagccgctt ttccgttctc ttccagaact ttgttagcat cttctacacc atactgtgta
     3961 cggtagatag cttctacgtt accaagctgt cccatgaaca tagcgtagga gatctttccg
     4021 tcgccgttca ggtctactgc atcgtagttg tcaaccaggt agttaccgat catttcgccc
     4081 tgcatatgtc cggcttccgg agcatctgta ccaacgaatg cacacttgtc atagcttcca
     4141 agtacttttc cttcgtcttc gtcgccttca actgcacggt tgaagaagat tacagggata
     4201 tctgctgctt ttgctttttc aacgatctga gaagaagcgt ctacagaacc agatgttacg
     4261 atgttaacga tcagtacgtt tgcacctgtc tggatagctg tatcgatctg ctcgttctgt
     4321 gttgtctggt tgttgtttgc atcgtagtcc tgatatttaa cacccagttc gtccagttta
     4381 gcatccagtg ctgtacgtac ggaagcgatg tatgtatcgc cgtatgtata gtacataact
     4441 gctacgtctg cgttagacag gtcaacatcg cctgctgcat ctgctacgga ctgtgcttct
     4501 gactcagcat cagctgctgc tgtatcagct gttgtatcag ctgctgcttc tgttgctgct
     4561 gctgtatcag ctgttgtatc tgttgtgttg ttagatgatc cgcatcctgc aagcagagat
     4621 gctaccattg ctgttccaag taaaccagcg attacttttt tcttcatgat aagttcctcc
     4681 caaaaaaaat gatttgtttt tcattactct aacaatatag cacaaagttc tcaattttcc
     4741 aatatgataa tttacgattt tgatataatt ttctattttt ttcttaagat ttaaaccacc
     4801 gtttcatact tttgcacgaa aacgagattt aatttttgtt acttttttcg aaagcaaaga
     4861 gtcttctctg acattcttca tcatacatgt tctctttgtc cactgcgatc gtatccgtat
     4921 aaatggtatc tttgatggaa tatccgttga tcagcttgta ggcggtctcc acgccgagat
     4981 accccatggc ataggaattc tgtacgatca gggtgtccac ttcccccgtc tccagcatgc
     5041 cgaccgaaac cacattgctg tcaaatgcga ccacacggac attgtctttc agcccaagtt
     5101 cctggattgc atagccgacc ccgagtgttg tccattcgtt aaaggtgacg atcacattga
     5161 tcttcggatg ctttttcagc attttctttg ttcgtttttt tgcattttct gtatcggaaa
     5221 gcacattgat cacagcatca attccggctc tttcatcact cagaatgcta tctcgaaatc
     5281 cttcttctct tttctggcca ttgtccgagt ttttatcgta attcacaatg ccgatatgga
     5341 tttcttcgtt ttcatctttc atggctgctt ccgctgcctt acatcccgca tcgtaattat
     5401 cggttccgat ccggcagctg accgcatcgc tgtccacgtc gctgtcgatg accacgacct
     5461 tgaccccttt gcctgccgca tcattgatcg catcggcatt tgcctcataa tctaccgcag
     5521 aaaaaataat gacttccgct ccgtcttcca ctgcctttgc aatcatcnnn nnnnnnnnnn
     5581 nngaccgatt tccagaatcc ggaaacggtt gactttgcaa taaaaacaac tttgtgcttc
     5641 tgatcgctgc tgctttcact ttcttttccg ctgagaatcg gacggtgaaa agccgtcaga
     5701 cagaagatca gcaccagaca gaccagaaat gccatccaca ggatgcggtt tctccaccta
     5761 tttgtcttca tagcttccct ccgttacttt cggcatcata atcgtgacac aggtgccttc
     5821 atccagctca ctctttatgg tcaggccgta ttcttccccg aaatagatct tgatacggtc
     5881 atttacattc ttgattccga ttccggcacg gtcgcttcgc tcctttttca gtacttcctc
     5941 gcactgttct tctgtcatgc caactccgtt gtcctccacc gtaaagatga tcttgtcttc
     6001 gctctgatgg atgctgatcc tgatcatgcc ctcatctacc atacgatcca tgccgtgata
     6061 aattgcattc tcgatgatcg gctgcagtgt gattttatta cagtaataag aaaggcatgc
     6121 ctcatcaaca tcaaattgat accggaactg atttttatag cggaaattct gaatatgcag
     6181 atagctctgt gcatgctgca gctctttctc aatcgggatc agctcatgtc cgcggctgat
     6241 gctgatgcgg aacagccttg ccagggcatt gaccatctcc acggcatctt cattgcgtcc
     6301 ggcttcgcac atccaggcga tcgcatccag tgtattatag aggaaatgtg gattgatctg
     6361 tgcctgaagt gccttcagtt ctgtttttcg aaggctgatc tcttcctggc ggacttcttc
     6421 catcagcttc tggatccgta ctgccatatg ttcaaacgaa tccgacagag cggaaatctc
     6481 tttggttccc gtcaccggat gatagacaaa ctcggcactg tttccctcga attctcccat
     6541 cgcacgcacc aggcttgtaa ccggttttga gaacagatcc gagaaaacgc tgccaaggaa
     6601 caccgttccg gcaagtacga tcgccaggat cacgaccagg ctgctgacta cgcgttccac
     6661 tttgtttgtg atcatttcat ccacatagca tactcccacg atatgccagt cgcagttctg
     6721 aagggagttg acggtgtaaa tcacattaga cttgatgtac gtcccgttct ccatattttt
     6781 ctggttttct tcttttaaag aagcgtaaat gagctgctgc tgcgggtgat agacaatgtt
     6841 tccggcatca tcggcaatat agcagtaacc atgctgcccg atcccgacat catccacata
     6901 atcggcgata ttgtaaaaac tgatatccac cgctacctgg atcgtattcc cgtttgcatc
     6961 ctgcatgttc tgggaaatcg ttaccaccca tggatagtag tccacaaaca gggnnnnnnn
     7021 nnnaacaggg attctacatg cggttttgtg atatttaaaa ttccttcttc ctgcggcaga
     7081 tcacgcacat aagaaagatt tttgatataa ttttctttta atttctgacc atttgtccag
     7141 cattttaaga tcttgccgtt catatcatag gaagtaactg caacaacatc ggatcttatc
     7201 ttcagaagat tggagaaaaa attatttgct tcttcctcct ctttctgaat gttagcacag
     7261 atcatctgca tgatatcgct catgtcttcg gtatagttga aaacggtatt ttttacctgt
     7321 acaacggcct gctcgctcgt tgtgatcgca ttctgctcca tatcattacg gtaaagattg
     7381 acaaatataa gcagtgatac gactgaagtg atcataacga tcacgctcac caggatgaca
     7441 aacagactcg acagtgaaat ctgctgaaac cgcttcaccg cctgtttaat cctgtctgcc
     7501 atcttcatac gttctcctgc agctgttcgg tgaaacaccg gtatattttt tgaagcaata
     7561 gctgaaataa tgctgatcgt tgtagccgca tttttccgat atctcacgga ctttcatgtt
     7621 ggtgtccatc agaagctctt tggctgtctc gatacgcttt ttcgtaagca gctctttgaa
     7681 cgtctcaccg gtatcctttt tcaaaagtgc acttaagtag ttcgggctga cggaaatctc
     7741 ggtgctgacc gagacaaggg acagatccgg atcggcaaat ttctcgttga tgatgcggat
     7801 cgcacggttg cagagcatca cactgctttt ctttcgctgg tctgcgatca tcaggcgtgc
     7861 cgtagtgcag atatctacat agaactgcct ggaatctccg atattgccga acattttcat
     7921 gctgtacatc gggaacttct gttgcatctg ctgaaccact tcttctccgg caaccgaata
     7981 aagtacctga taaaccgtcg aaacgatcag catcaggata aaatccgtct cagactgaga
     8041 gatcttgtgc tgtacgacct ggtcaaaaaa gttctgcaga tagccgctga tctcctccgg
     8101 tctgcctttt cgaagcagat tttcgatatc ttccacggtc tgatgcacca tcaccttgtc
     8161 aaagctctct gtccgttcat cgtctgatat aaaatgaatg tttccttctt cttttccgta
     8221 atttgcagcc gcactcatcg cctccagata gcactcatga catctttcca gcttttcgac
     8281 tactctgctg actccgatca cgctgtccat ccccatgata cgctccacac tctgacagat
     8341 gtcatctacc agaatatgca gatatttatc aaagccacgc ggcgttgcca gaagcaaaga
     8401 tactacgcgt ccctgtaaat agacgctgca gtacttggtg tactttttga ggatggaatc
     8461 gatcgccttg acgctgcctc ttgtcgtgca gttctttccg tctttatcca ggattctggt
     8521 gaccnnnnnn nnnngtgacc atcaccgcat aattcaaggc ttccggttct tccccgtcga
     8581 gcagtccgca ggaaattgcc tctttcatca gcatcctgtg ctcttcttcc ggaagttccc
     8641 ctccaaagcc atccagaagc aacggtgtca gaaactctct tttctccata tgctcctgac
     8701 gcatccccgg ctgggaaaat acctgaaact tctgatcgat cttctctttc atcttcagga
     8761 gctctttttc aagttcggaa gatgagatcg gttttaacag atagctgata atattatatt
     8821 gtatggcctg ctgtgcataa gaaaaatcat caaatccact cagaaatgcg atctgggtag
     8881 ccggccttat ctcccgcacc tggcgtgcaa gctcgatgcc gctgataaat ggcatgcgta
     8941 tatccgtcag aagcagatcc ggctccagct tttccgtcag ttccaaagct tccgcaccgt
     9001 tttccgcctc tccgatcacc tgaaaaccga cgcttcccca gtctactttc cggatcaggg
     9061 aacgacggat ctcttgctcg tcatctgcaa caatcactgt gtacatggtg ttctccttta
     9121 ctaacttttc ggtattctca cagttacctt tttccttcca ttatacccag aattgcagat
     9181 ttgtcaaaaa aaaggaggac ttttcagtcc tcccacgggc gaatgcggta ttttgcccct
     9241 atgcggtcac agatttcctg acactgcttt tnnnnnnnnn ncgaatttgc tgcgttccag
     9301 cctgttgtac tcatctgcat tcgtggtatt gaggctgaca gaaaccgtat ccacaaggcc
     9361 tttcagttcc ggaacgatgt ctcttccatt gaccaggttt ccaagtccgt ttgtgttgat
     9421 acgaatcagc atattatatt tttctttgat cagctttgca ctctgtttca gcacatcgag
     9481 tgcctcggtc ggttcgccga atccgcagaa taccacttct ttatattttg aaaaatctac
     9541 ggctttcatg gcttcctgca cttcttcaaa agatggtgca tgctccagcc acaaaacatg
     9601 ggaattttcc atgtgatctc tggtctgacg caggcagaag gtacaggcac atggacattt
     9661 attggtgaga tttacataga tattatcgtg tacttcatag aaaattgtca tttctttttt
     9721 cataatgcag cattcctttc agtctgtttt taattttcct aaaacttctt caaaacatac
     9781 gccgtctgtc agcggaatat ccagttcctg tgagcggata atacattttt tgataaaatc
     9841 ggaagctttc ttcacggatt tatagaacgg cactccgttg acggcatcgg ctgcgatgat
     9901 ggatgcaaag atatcgccgg ttccggaacg ctgtgttcct tctttgtggg tacgcagcac
     9961 tttcggctgc tgcccttctt cgtaacagaa gtttgcaata aatccgccct ggacgatacc
    10021 ggtgatggcg atttttttcg gtcccatcgc gtgaatcttc tcggccattt cctggatttc
    10081 ttcgatcttc catttttctt tgtac
//
LOCUS       DAWDWH010000055        10033 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3402_length_10033_cov_11.092904, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000055 DAWDWH010000000
VERSION     DAWDWH010000055.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10033)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10033)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10033
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3402_length_10033_cov_11.092904"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3606..3615
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3292 a         1650 c         2237 g         2844 t
ORIGIN      
        1 gggacggagt ttacttgatc catttaggat ttatgcgaat cagacaggta cataccagta
       61 tatgcatgtg aattttccga gtttttggat gtttgtagga aatgattatg aatggctgaa
      121 aacggcagca atcgttatgg cggcaggcat atgtggtatg ggattgtata ttattatgaa
      181 aagaaagtta gattgcagtc gtcctgaaaa ttttgtgagt attgccagtt ggtttatctg
      241 gacttgtgta ttgtttttac cggcaatgca tgaaagatat gcatatccgt tggaaatact
      301 tttggttata cttacttgca tcaataaaga ctatttaaaa tattcttcgg tggcaatatt
      361 atttagttta tttacttatg gaaattatct gttttcaaat gggggacttg acaagtatat
      421 agtaattatt tatgcaggat gttatttaca ttttacatat acaattctaa aaaagaacag
      481 taaggcggct acagtaacag acaaggagat gcaaaatgaa taataaaatt ttacaggaaa
      541 gtgcagacga ttctatttta caggaagacc tggatcgtat ttcagagagt gatattccat
      601 tggaagagct gaagggcaag acagttctgg tgacaggggc gactgggctg atcggttctc
      661 tgctggtaaa agcattgcag tgttgtaatc gaagaaaaga gacaggtatc aaaatcctgg
      721 cacttgtgcg taatcaggaa aaggcagaaa gactttttgg tgatctgtta aagagagagg
      781 atttaaagtt ggtgatcgga gatattacga tgccggttga agtggatgat aagattgatc
      841 atatcatcca ctgtgcgagt attacaactt caaaggttat gatcagcaga ccggtggacg
      901 tgattcggac atcccttaac ggaacggaga atcttctgga acttgcaaag gaaaaacagg
      961 tcagaagcat ggtttatgtt tcttcgatgg agatgtatgg ggcattaaat ttacagaatg
     1021 atcagatggt gacagaggaa atgacgggct atttaaatcc gctggcatta cgcagcaatt
     1081 atccggaaag caagcgtatg tgtgagaaca tgtgtgttgc ctatggaacg cagtatgaag
     1141 ttccggtgaa gatcgcaagg ctggcacaga cgtttggtgc cggagtattg ccgggagaaa
     1201 accgtgtttt tgcacagttt gcaagaagtg ccatgaaggg cagtgatatt gtacttcata
     1261 cagaaggaaa atccgaagga aattattgct atacggcgga tgcagtacgg gcattgctga
     1321 ccattctggt taaaggcgaa aatatgcagg cctataatgt ggtgaatgag aaatcgcata
     1381 ttacgattgc ggatatggca aaatttgttg cagagaagat tgcagaaaag ccgatagaag
     1441 ttgtatttga tattccaaag gaaaataaat ttggatatgc cagtgataca aaaatgcatc
     1501 tggctgccac cagattgatg gcacttggat ggcagccgga aacagatctg gaagaatcct
     1561 atcgaagact gattgcgagt atgaaaaaca gaaatgttta aaagtatttt tatagatttg
     1621 gcagtaaatg attcgatttg tctgaaatag ttacacagtc ttccgtaatc gttcagacat
     1681 caaaatatcg gtcaagctta cttaaggatt tgcgtatgtg aagcaactga aacagtttgc
     1741 ttcgaactgc atctacacag cagccaacaa caaaaatggc aaggataatt ccgaaaaaga
     1801 ataagagcat ttttgaaaca gaaccatttg tgataaaagc aaaacgctct gtcataacat
     1861 aactgcggac aagaggatga tcatgcaaca gatagacacc gaaagaaaga ggtgatatca
     1921 gcatgatcat ttttttgacg gactcggaaa agttcatgct gccgaaaatg aggagcaatg
     1981 atatggccgc cagtacaata aaaggtgatg tatagtgaat caaaaaagaa gaatccagag
     2041 taaatccgct gatcggaaat ttttcaacaa gaattttgct gctccatgta attaaaatgc
     2101 agaaaaaata taagataaag tattttttct ttttagagtg agagaccaga ttcagttttt
     2161 tgatgcacgc accgagaagg tacatcagtg tgagccagaa aacactgtat ccgtcgccca
     2221 tataaaaaac atcctgctgc ggtgtaaaag tctgtgaaat ggtcggaacc acagaaaata
     2281 cgacaagagt aaaacagaat aaagttttta tttgcttttc ggagagattc cgaaacatct
     2341 gattgatgaa tggggagaga aaaaacactc ctgcataagc agtaaaatac cagtattctt
     2401 ttcgggtgac cggaaagagt gcctgtatcc agccggatgt atttctgctt tccggaagca
     2461 gaaaaaagaa aacggtgttg atcaggattc cataaaataa gacctgaagc caaagaatca
     2521 gaatgtttga aatcttaaaa tgagaatcaa cactgacaaa tccggatatc atagcgtaag
     2581 tatttacagc accgaaggca atcacttcca gcagccacgc aatctgataa tgtaccgata
     2641 aaaaagaaac agaacgtaaa ataccgccat gccccagagt gtggagcatg acgatcataa
     2701 tcatagaaat cattcgaaga aaatcaatac cataatttcg tgttttcata agtcttttcc
     2761 ttttaaagaa atcaatcctg aatcttgtca acagtaatat ttggaaaaag tgcatacccg
     2821 acagagagac tgttcggatt gcagtaagga atcttgttat ttccattgaa ggttcctgag
     2881 caggtgatct tcttatccag ataggaaaca tagtctttca ttttcatgga taatgaatag
     2941 atatgtcctg tgccctcttc agataacagc ataaccagat tgtttgactc agcatcagca
     3001 ttttcatata caactttgcc ggttacggaa acttttgagt cagcataata aagaggatat
     3061 ttgcagattt ctctgtaatc tttcgtcagg tgcatcggat cagcggtggt gtcatcgtct
     3121 tcaatctccc cacagatcgt ggtaaattcc ggaagagaat caagagcttc gacatccgta
     3181 acggatacca gatcgctgta ttgtttcatg gcatcttctg aaataatcca gtccttaaag
     3241 gaatcaaatg actttttgta accgagcagg cggtgatata aaatggtaga gctgtccgaa
     3301 atctcggaca aaccattata aacgccgtaa atttttatcc tgtcacctac ggaaagattt
     3361 ggaatacatg gagtagcaaa tttatctgtt tttaatgtta ctgcacgaaa cagataagga
     3421 ttgccggcat catctttgat cttgaaatag gcatattttg tgctttcgta gatttcctga
     3481 acttcacctt caacctcaat tggatgatca acgtgatcgt aaggattttc tgttaattct
     3541 ttataaggta tagaccgggc atttgctttc cattctgaga ttggagtaca aagataaaac
     3601 cagtcnnnnn nnnnntgctg ctttggaatc ggcagaatag gatgtaattt ttttgtatgg
     3661 ttttccggca atggaagaat atttcaaaaa aatgccgtca gggtctgttt cttttatttt
     3721 tcctgaaatc agaccgtctt tgtactgaac ttctgctgat tctccatctt tgtaagaaat
     3781 gacacagtca ccggaaaaag catcgtcatc ccatcttcct tctatctgaa gaaaatcagc
     3841 ggatttaccg gcaggagtga aaatagcttc tccggcatca gcattcttcg taacggttcc
     3901 ataatatact ttattaaaaa catttacatt tacgaactgc tcgtttttac tgtctgctgc
     3961 actggtgtga aacgaacata caccggctaa aaccagcagg caggcaatcg ctttttttaa
     4021 cattatagat cgctcctctc ttctgagtat tttactaata aaaaggaaat cagtcaaata
     4081 ttacttctgt ttttgaagga atcataattc actcacattt atattattaa atcaattatg
     4141 ggagagtaca tattactgct gcgtagtttt tcgggtcttg tttttaacag tattgtttgt
     4201 tataatcaat aaccagagat taaaggagaa agaggatgtt tatgggcaaa aagcgaaaag
     4261 ctgtcatgct tggagtaaat gtatttcttg tactggcact tttatggtct gtagttttta
     4321 ccgttgaccg aaagaatgcg gcctacacca atacggacat agaacgatac aagagtttaa
     4381 ataccatacc aaagcttgga gctgtcacga acagcagaac agctgagatc caattttcat
     4441 tgtacgatga aaaattatat ggcatagctc tcttttttta tgtatcggga gaaaataccg
     4501 atggagagct tgtatgttca ttgaagcacg atggagagac tatttcagaa cagacggttg
     4561 cagtgaagga gctgtttgct cttgtcaata agaatgaaat taaaccaaaa gaactgattc
     4621 ttggaaacgg tgaagtatgc aatggcgatt atacgctgga aataaaggga agtggaattt
     4681 catcgcagac aaggatttct ttatatggta ataaggcaga tgaatatttt ctggattata
     4741 ccaatgcaaa ttatcagaac tataatggaa ttctttatat tgtggaaact ttagacccag
     4801 aacgaccata tgtatgggcc tgtgcatttt tactggctat gagttttgtc tttagcctga
     4861 tcatatgtat gaatgagagg gagaaagaac atgaataata ttaagaaaca taagagactg
     4921 atagctgcga ttgttgtgat tctgctggca gtgctggtag agatttttgc aaaccggtct
     4981 gcatggggac atgcatatga tctggatatt accgatcata tgtacatcag cgagagtgca
     5041 ggtcaggaag aatatcaggt tatattttct gctccaaaag gactttatat tcagatgctc
     5101 catgtgcagg ggcattttaa agatgaagag ttctatgata tagaaacaaa agaaatcaat
     5161 gattttggca aagaaacaga gcaaaaatat catgactgtg taaataaact tctgccggaa
     5221 tattcaacca gcatcaacaa aaaagtcacc tatctgaaga tgaccatgaa gaagaataag
     5281 gatgcagaac tgacaaaagt atctctgtcc aataaagcag acattaatct gtatcgttat
     5341 ttctttatac tgttgatcgg agcgatgatt tactgtactt tctttgaaaa agaatttatc
     5401 aagaagacag aatggtattt cgcagtattt gcactgagtt ttggcatgtt gatcattgca
     5461 gcggcccagc caacctgcaa ttcctgggac gagcagattc atttccggga ttcttatcga
     5521 attgccagcg ggcataatgt agaatggaca gaagccggac ttaacattat gaaccgcatt
     5581 aatccaaaat gtaatacaaa ggaagaatat aaacttctga aagcatatat ggatgaacat
     5641 ggcacggaag ttgttatgaa agagacacag gaaacgtcag gaatttctta tgataaaatt
     5701 gcatatatcc cgatggcagt ttttctgttt atcggaaaga atttgaaact tccattttcg
     5761 gcagtgtttg cacttggaaa attcggcaat cttctgatgt atgtgattgt gatgttttgg
     5821 gcgattcgcc ttgcaaagag cagaaaatta ctgctggcgt ttgtagcgat gcttccgacg
     5881 ccgctgtttc tggcatcgtc ttatacgtat gacagcgttg tgttctcgtt cattacactg
     5941 ggatgtgtcc tgtggtcaag agaagcattt ttccagtcaa cgaagtatta tcatacagct
     6001 tctgtgatcg gagcaatatt tatgatgtcc gtcggatgcc tctcaaaagc agtttatatt
     6061 ccactggtat tgctgatgct tctgctgccg cagttttata aaaagaataa aaaagaaaaa
     6121 atcttattcc tgatcggaat cggagtatta ttcctggtag ttatggcaac gtttgtatta
     6181 ccggttattt ccaatacagt gtcagggaat attgcttatg gcggcgattc acgaggcgga
     6241 gataccagcg ttgtaagaca gctggtatct atggtaaaac atccgctggc aagtatccgg
     6301 cttatgttcg gaagtatttt ccagctggac aatttccgaa atctgggtaa tatagattcc
     6361 gataattatt tcttcggaaa tctgatgttt ttaaattatg catcaagggg aacacttgca
     6421 gataaatgga gtatcctgtt gcttccgatg ctgaccctgc tggtttttca gaaagaaaag
     6481 ggcagcagca gactggcaaa ttgtgaaata tggaaacgaa tcctgatgtg gggcattttt
     6541 ctgatcacgg taatgctgat ctggagtgca atgtatttaa gttttacgcc ggtaggagag
     6601 gcaagtatcg ccggtgtgca ggcaagatat tatctgccgc ttatttatct tggagcatta
     6661 ttgctttcaa attcaaaaat aaaagtagaa atcagtaaat acggactggc aaaactatca
     6721 tttattgttg caaatatact gcaaattgca gcggtatggg aattattgct gcagggaaga
     6781 ttgatatagg ggaatagaat gacaaaaaga aatgatcata cgtttgcaat ttgtgcatac
     6841 aaagaaagtc catatttaga ggaatgcata ctttctctaa aaaatcagac ggtcaaaagc
     6901 aatatcataa tggctacttc aacgccgaat gaatggatac agggactggc agagaaatat
     6961 gagattccgc tgtacatcaa tacaggtgaa ggcgggattg cccaagactg gaattttgca
     7021 tacagacagg caaaaacaga ctatgtcacg atcgcccatc aggatgatat ctatgaaccg
     7081 aattatttga aaatgatttt cggagaactg aaaaaaggta aagatccgat tgttgtgttt
     7141 accgattatg gtgaactcag agatggagaa aaagttcaga aatcaacgct gctcacgatc
     7201 aaacgtattt tgctgctgcc gatgcgagtt cagggattcc agaatgtgcg attttttaaa
     7261 cgcctttttc tgaggttcgg caatccggtc tgctgtccgg cgacaactta catcaggaaa
     7321 aaatttaagg gggatccgtt tgaagtaaga ttctcaagca acgtagactg gatgatgctg
     7381 gagaagcagt ccagagaaga gggatctttc tgttatatat cagacatagg tatgtatcat
     7441 cgtattcatg aaaaatcaac aacgacagaa attattcatg gagatatccg ctggagagaa
     7501 gactacgaaa tgtttcgaaa attctggccg gcatgggttg caaaaccgct tgtcagggtt
     7561 tatcgtttaa gtgagaaatc aaataatata caacagaggg aagagaaatg aaggtattag
     7621 tgatcattcc ggcatataat gaagaattaa atatcaaaaa agtagtgtct aatttgatcg
     7681 atcattatcc acagtataac tatgtagttg taaacgatgg ttccgcagac agcacatcgc
     7741 agatctgtca ccaggaaggc tacaatatca tagacctgcc ggtcaatctt ggacttgcag
     7801 gagctttcca gacaggactg aaatatgcag atcaggaaga ttatgactgt gcgatccagt
     7861 ttgatgcgga cgggcagcac agaccggaat atatcgaaga tcttttaaaa gaaattgaaa
     7921 acggatatga tattgtgatc ggttcccgtt ttgtgacgga gaaaaaacca ttctcgatgc
     7981 gaatgttagg aaatcgtatg atcgaaagag caatcaaatt ttcaaccggg aaggtactga
     8041 ccgatcctac ttccggaatg cgtatgtaca gtaagaagat cattcacgaa tttgcatcca
     8101 atatcaacta cggaccagag ccggatacca tttcattcat gatcaaacag ggagcttccg
     8161 ttaaggatgt gcaggtgaaa atggatgaaa gaactgcagg agaaagttat ctgaatctga
     8221 caagatcgat cgcctatatg ctcagaatga tgctttcgat tctgtttatc cagaatttca
     8281 gaaaacgcag aaaagcagac taagaaggga gtcaatatgt cgatctattt aagaattatt
     8341 ctggttgtca tatccatgct cagtatgatg aacgttatga agagagtgag aagattcaaa
     8401 ctgcagatag aaaactctat tttttggatc attttttcac tgctgctgat actggtggca
     8461 gtcttcccgg atccaatgtt ctggtttgca gagcttttgg gcatccagtc tccggcaaat
     8521 atggtatttc tctttgttat ctttatcctt ttaattaaga catttaatct gacacttgaa
     8581 atttcacagc ttcagtacaa aatacaggag ctggtacaga aaatagcatt agatgaaaat
     8641 aaaaagaaca aagaagcgga caaggaggaa aaatgagcaa agtatctgtg attgttccgg
     8701 tttataatgt agagcaatat ataaaacgat gcctgaaaag tattctggat cagagctggc
     8761 aggattttga gattttatgt gtggatgact gtggtcagga tcagagcatt tctattatag
     8821 agcagatgca gaaagaatat ccgcagaaaa taaaaatatt atacggggaa caaaacatgg
     8881 gcctgggtgc tgcaagagat cgaggaatga aggcggcatc cggggaatac attgccttta
     8941 ttgatagtga tgattatctg aaacgaaatt ttctggaaac ttatatgcat gcagcacaga
     9001 aaaaagatgc agatatcatt gttggcggtt atatccgtga tgtaaacgga aagttatcgg
     9061 aaacaccgat cgatgtcaac gatgatacat ttgtatggat gaatgtcagt gcctgcacaa
     9121 aagtatataa gagaaaattt ctggaagata atcatctgtc tttttatgga gtaagacgct
     9181 acgaagatga aggatttatg tatcgatgtc tggcatgtca tccggttgtt gaacttatgg
     9241 catattccgg ttactattac tatatgaata gcgagtctat taccaggagc agcaagagcg
     9301 accgaaccgg tattgtaaaa gaatattttc agacagtgaa caaactggct gataccgtaa
     9361 agtgtgacag agagactcag ccattgttaa attattgcct ggtatccgga ctgacggcaa
     9421 atatgcttta taatggtcag ggatgcggcg gaaaaaaaat gaaagaatta tatattcagt
     9481 ataataaact gctcaataaa atagacagag atatctgcaa aaacaaatac attgcattga
     9541 agtatttaaa atcagaaccg gcaaaaaaga gatatacaac atggctgatc ttaagactga
     9601 gaaaaatcgg actggacagg ctgctgtttt ttctggtaag caggatctga taaaagtaaa
     9661 gctgaaacaa tgagaggaat tatcagatga aaaataaaaa attaagctct gaagaaatca
     9721 aacaaataga actgaacctg ttattaaaac tggatgagat atgtaaaaaa tacaagctca
     9781 gatattacct gtgcggagga acacttcttg gtgcagtccg tcataaaggt ttcattccgt
     9841 gggatgatga tatcgatgtt ctgatgccgc gtgaagattt tgagaagctt ttgagactgg
     9901 aaaaaaagca gaaacaagat gcggtggaaa agattgtttc ctggaaatct ggtaattcta
     9961 tctatccgtt tatcaaactg atcaatacga atacggtact gaaagaaaag tatctttctg
    10021 aagaatttac aac
//
LOCUS       DAWDWH010000056        10032 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3403_length_10032_cov_14.192743, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000056 DAWDWH010000000
VERSION     DAWDWH010000056.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 10032)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10032)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10032
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3403_length_10032_cov_14.192743"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1823..1832
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2893 a         2000 c         2598 g         2531 t
ORIGIN      
        1 attctggtca gcgaaagctg acatattctt cttagaatca ctgcacattc tacacaagaa
       61 gcaggtttct cgatctgaga tccctgcttc ttgttgtttt ttacctgtaa ataccgtata
      121 cctgtttaag aatgtatctg gcatacttac tgtgaggtgc acgtctatac atcacatgat
      181 atatttttct gtgcacattt gaggtatgat ttagaaacct gcttctttga gcactttctc
      241 agctgctgcg gtgtcgttgg taacacacag aacagcgtag tttccggaag tcttgatgat
      301 cgcattgtcc aggttcttta cttcatctgc gttgtaggaa gagtaaagat cggactgatt
      361 ttttacacgt gtttcaaaaa gtccttttac ttctgaaaca taagagctgt cgctgcattt
      421 gatgacaaca gcttcgcatg cagtagcacc ggtaccgacg taagcagcac aggaatcaat
      481 tttggaagtg tctaccaggt aagtggaagc caggatatct tcggaaatag aagaaagggt
      541 gtctccgctt gctgttttgg aaagatcttc tgcaagtttt gcagggtctg ctgtgacttc
      601 tttgccggaa ccgccgcatc cggtcagcat gacaccgatc atagcgatgg tcacaagaag
      661 tgtgatgagt tttttcatgg tttctaatct cctttgcttt atgttttatt aatgtgagaa
      721 cagactttca tctgcctcaa ataatgttcc gaaaacggac aagctgaaaa aatcataccg
      781 tttcggaaac atgatttctg tatgctctgc caggttacaa agaagcatcg gaagcagttt
      841 cactctcaga tgcagtttcg ctttcagaat cactggaact gctgtcagct gtcacataat
      901 gcgtgtgcag ataatccagc cattttgcac agtattccgg atacatatgg ataccgtcgc
      961 tggtagcatc ggcggcaagt gcaccgtttc cgttggataa cacttcgttt ggattcagat
     1021 aataataacc attggcactg cagatttcaa ggataatctg gttgaccgtg tcaatgttag
     1081 tgttgtttac atagtcgccg gtctgaactt tggcttcctc cacgtatggc acggcactga
     1141 cataaaaaat ggcattcggc atcagtttgc ggagttctgc aagacatttt gtataattct
     1201 tgcggaattc ttcaaaatcc ggataaccaa ggtcgttggt accgatcatg atgtacattt
     1261 ttttgaaaga attgtttttc agtgcctgca ttacggttac atttccactg gcagtagata
     1321 tgtacggtgt ctcaaaaata tccgtgcagt tcatgccgac accggtcagg aacgttccct
     1381 gcgtaatgcc ggactgcata tggaatccct cgactctgga atcaccgata aatacagcat
     1441 cagaaaagta agaatcatcc accggcgttg cctgtttttt tacaatgcat ctgtcgtcca
     1501 cagtaacgga agaagtggag gtagatccgg ctccgtttac aaatggtgtg acgttggcag
     1561 gatatttgga agcggtttcc gatgaagtct cactttctgc cgtagcttcg tcctgattct
     1621 gaaaatcact ggaagagaat attttgttga gttgctcatc tacctgcttg tttatgccgc
     1681 ctttggaagc gatattgatg atcagacgac cttcaaaaac aactaaaaac gcaaagacaa
     1741 gcaggagcat attaagaaag cgggcggttt tacgcggctt tcgtcttttc tgctgtacca
     1801 taatctcccc tcttaatctt aannnnnnnn nnaaatagtt tttttagacg ggagttttta
     1861 attatggttt tcagcagtct gttgtttctg ttccggtttc tgccggcagt actgatcctc
     1921 tactatatag caccgcgaaa gcttcggaat gtcattctgc tgctcttcag cctgtttttt
     1981 tatgcgtggg gcgaaccgaa gtatgtattt ctgatgcttt ttacgatcac catggatttc
     2041 ttcatcggaa aagggatcga caaagcgaaa aaggaaggca atcagaaaaa ggcgaagcgt
     2101 tttcttatga cttcaatcct tgtggatctt tccatccttg gattttttaa atatgcggac
     2161 ttcctcatcg ggacggtcaa tacattaacc ggtgcgggaa taccactgct tgggataccg
     2221 ctgccgatcg gtatttcttt ttttacgttt cagacaatgt catacacgat tgatgtatac
     2281 aagggaaaca cggaggttca gaaaaactgg atcaagtatg gcacttatgt ttccatgttt
     2341 ccgcagctga tcgcaggacc gatcgtgcag tacaaaacga tcgcagagca gatggagcac
     2401 agaaaggaaa atacttccga tttttcagaa ggtattcatc gttttatgat cggacttggc
     2461 aaaaaagtcc tgatcgcgaa caacatcggt gcactctggg atgccattgc agtacttccg
     2521 gtcgatggac tggcagcagg tacggcatgg cttggggcac tggcttatac gttccagatc
     2581 tactttgatt tctccggata ttcggatatg gcgatcgggc ttggaaagat gttcggtttc
     2641 catttccttg agaacttcaa ttatccgtat atttcaaaga gtatcacgga attctggaga
     2701 agatggcata tttccttaag ttcctggttt aaagaatacg tttacattcc gcttggcggc
     2761 aaccgctgta agatcgcaaa gcagatccgc aacattctgg ttgtctggat gctgaccgga
     2821 atctggcacg gggcgaactg gaattatgtt ctctggggct gctactacgg agcacttctg
     2881 ctgatcgaaa agctggtgct gaaaaaggtg ctgaaccgtc ttcctggaat cattcagcgg
     2941 atttacacca tgttttttgt ggtgatcggc tggatcattt tcaaatgcga agatatggca
     3001 tatgggatct cgtatctgaa agccatgttt ggcggattcg gacagggcct gtggaataac
     3061 gagacactct acctgcttgg taattatgcc gtcctgtttg ttctggcgat cctcggttcg
     3121 acgatgttgc caaagaaagc agccggcgtt gtgcttggcg gcagaaagcg gacctgggcg
     3181 gaaaatgtga tctacattgc attttatgcg gtgatcttct ttgtgtcagt cgcttatctg
     3241 gtagatgcaa cgtacaatcc gtttttatat tttagatttt aatgatgcca aggttcaaag
     3301 gtcaaaatgt cgttcttgct tgcaagataa gacatttgaa cttggaacct gctaaacatg
     3361 aggaagtagc tatttacgca gtaaatatgc ggattccgaa tgtttataga attgtgagag
     3421 tacgaagtac ggaacaattc gtaggcatct ttgtacatta acataacatc tttaaacagg
     3481 gaaacagaat ggagagaata tatgactaca aaaaagcaga atatagcagt gatcgctgtg
     3541 tttctgtgca tggtcttcgg aatgacaatc tggacgatgg tcacgccttc cagagaattt
     3601 tcggagaggg agaacagagc actggcacag atgccggaag catcggttga aagctgcttt
     3661 tccggaaaat ttgaaaaaga ttttgaaacg tatctgacgg atcagttccc gatgcgggat
     3721 ggctggatca gtgcaaagac tgcgatcgag aaggcaactt ttaaaacaga gagcaaggat
     3781 atatattttg cagacgatga ttatctgatc gaggcacatt ccaaaagctt tacggcaccg
     3841 agtgcaaatg ccaatatcgg ttacctgaag aattttatgg aaacgtgtgt ggaaacatac
     3901 ggcaaagacc atgcaacagc gatggttgtg ccgaatgcag tggacattct gagagatcat
     3961 ctgccgaaat atgcatctcc gtacgatgaa gaagtctacc ttcagaaagt tgcggatgca
     4021 atgccggaag gcacctggtt tgacagcgga agtgttctgc gtgagcataa gaatattcag
     4081 ctttattacc gcacagacca ccactggacc acggaagcag catattatgt gtatgcggca
     4141 tgggcagaag aaaagggact ttctccgctt gcactgtctg attataaaaa gaagacgctc
     4201 acagatggat tcttcggcac ggttgatgcc aaggtcggcg gacgcaaccg cagcgatacg
     4261 atccatgttt atattccgaa gaaaaaggaa aaatatcagg taaattatgt ggcgaaaaac
     4321 gagacggcag attatctgaa aataagagac aaatacagcg tttattttgg cggaaatcag
     4381 agtctggtgg aagtaaagac agacagcaag agcaaacgga agcttctggt cgtgcaggat
     4441 tcttacgcac actgttttat tccgtttacg ctgcatgatt ttaaagaagt cgattttgtg
     4501 gaccttcgct attacagcga aagtctcaaa gaatatatgg aaaagggcga ttatacggat
     4561 gttctgtttt tgtataatgc ggccggattt gcagaggaca acagtttgat caaacttgga
     4621 aactgaaaac ggggtaaaaa agacaggagg aaatggcatg agctatgcag ataaagtatt
     4681 taagggaatg tgcagagata ttctggataa cggagtgagc acagaaggag aaaaggtgcg
     4741 tccggtctgg gaggacacga atgagtcggc gtacacgatc aagaaattcg gcgtggttaa
     4801 ccgttacgat ctgcgaaaag aatttccggc actgacgctt cgaagaacgg cattgaaaag
     4861 tgcgtttgat gaacttctgt ggatctggca gaaaaagtcc aacaatgtgc atgacctgaa
     4921 ttctcacatc tgggacgcat gggcagatga ggacggctcg atcggaaagg cttacggcta
     4981 tcagctcggc gtgaagcatc agtacaaaga aggcgagatg gatcaggtgg accgtgtaat
     5041 ctacgatctg aagaataatc cgtacagccg ccggatcatg accaatattt atgtgcatca
     5101 ggatctgcat gagatgaatc tttatccttg tgcctacagc atgacgttca atgtgacaca
     5161 gaaaaagggc gaggataagc tgacgctcaa tgccatttta aaccagagat cgaacgatgt
     5221 gctgacggca aacaactgga atgtggcaca gtatgccctg cttgtgcata tgctggcaca
     5281 ggtgtgtgat atgtacgtcg gcgagctggt gcatgtgatc gcagatgccc atatttatga
     5341 ccgacatatt ccgctgatcg aggaactgat cacaagagaa gagcatccgg ctccgaaagt
     5401 atggctgaac ccggagaaaa aagatttcta tgaatttacc gtggatgatc tgcatgtaga
     5461 agattacgta accggaccgc agatcaaaca tataccggtt gcagtgtaaa agctggcatg
     5521 acagaataca tttaccggat aaaaaagtgt aaagaatgag aaaagaggag gataagtaag
     5581 atgaatttga ttgctgctgt ggataagaac tgggcgatcg gcaagaacaa tgaactgctg
     5641 gtcagtattc cgaacgatat gaagatgttc cgtcagatga caaccggaaa agtagttgtg
     5701 atgggcagaa agacactgga gagttttccg aacggactgc cgctcaaaaa cagagtcaac
     5761 attgtcctga caaaaaatca gaattatcag gtcaaaggtg ccatcatcgt gcacagtgtc
     5821 gaggaagtgc tggaagagct gaaaaaatat gacagtgagg atgtctatgt gatcggcgga
     5881 gagcagatct accgtcagat gcttccatac tgcgatacgg ctcatatgac aaagattgac
     5941 catgcatatg aggctgatgc ttattttcca aatctggatg agaaggaaga atggaaaatc
     6001 acggcgacga gcgatgagca gacgtatttt gatctggaat attattttgt gaaatatgaa
     6061 aaacaggtac agtgaagtca agtcgaacat acgtgagact tggtatgcga ttttggtcag
     6121 ctttgctgac attagggagg tctgatttat gtacagagaa catacaaaag tggtaaagat
     6181 cggcaaccgg gtgatcggag gcggaaatcc gatcctgatc cagtccatga caaatacaaa
     6241 aacagaagat gtgcgggcaa ctgtggcaca gatccaggct ttgactgcag caggatgcga
     6301 tattataaga tgtgcagttc cgacacagga agctgcgagt gcattgaaag agatcaaaaa
     6361 gcagattacg atcccactgg tagcggatat tcattttgac tataaactgg cgatcgcggc
     6421 gatcgaaaac ggtgccgaca agatccgcat caatccggga aatatcggca gcagggagcg
     6481 tgtgcaggct gtagttgatg cggccaaaga gcgaaacatt ccgattcgtg tcggtgtaaa
     6541 cagtggttct ctggaaaaag acctggtgaa aaaatacggc ggtgtgacgg cagaagggat
     6601 cgtggagagt gccctggata aagtgcatat gatcgaagat atgggctatg acaatctggt
     6661 catcagcatc aaatcttccg atgtactgat gtgtgcaaaa gcacatgagc tgatcgcacc
     6721 aaagaccggt catccgctcc atgtcggtat cacagagtcc ggaacagttt tatccggaaa
     6781 catcaaatcc gctgtcggac ttggtatcat tctttcgcag ggcatcggtg atacgatccg
     6841 tgtatctctg accggagacc cgaaagaaga gatcaaatct gccaagctga tcctgcgtac
     6901 gctggggctg cgaaaaggag gcattgaggt tgtgtcctgt ccgacctgcg gacgtacaca
     6961 gatcgatctg atcggtctgg caaacaaagt tgaaaatatg gtagaagata ttccactgga
     7021 tatcaaagtt gcggtcatgg gctgcgtggt aaacggacct ggcgaagcga aagaagcgga
     7081 tatcgggatc gccggcggaa aaggcgaagg tcttttgatc aggaaggggg aagtggtgaa
     7141 gaaagtgcct gagagtgaac ttctggatgc tctcagagaa gagcttcttc actggaatga
     7201 gattcatgcc taaagagttt tttgatgtat ttccgacttt aaaggtaaat gacaacttaa
     7261 gaggattact ttctcaggcg acggttacga gagtaaccgt cacttctgca agagacagaa
     7321 tgagggtata tctggaaagc ccgaggcttt tgtactggca gtccgtgcag gatacggagc
     7381 atgagatacg aagacagatt tttggcaatg catcgatgga cgtaaagatc attgtcaaat
     7441 ttcagttatc cagacaatat acgccaagaa cgctgatgca ggagtatgag agcagtattc
     7501 tatctgaaat cagagattat aatatcttcc tttacagcat cctgcgtcag gcagaatgta
     7561 ctttcacagc ggacgatgag atgacgctga ccatcgaaaa gaatgtgata gcggaagagc
     7621 gtcttgagga gcttttgcag atcctggaga aaattttctg cgaacgctgc ggcatgcatt
     7681 ttaaggtgca gactgttttc aaagagccgg tggagagcaa gtcgcataaa aacagcgagc
     7741 tgcgtattca gcaggaagtc gatgcgatcc tgcaaaatgc cgtgcttggt aatccggaag
     7801 agcctcagaa tctgccggaa gaaaacggac aggtggagtc tgcaaaagcg gaaggaaagg
     7861 cagaagcggc gaaaaaagag aaagcaaaac cggaaaagaa aaacgacgga aagagcgaga
     7921 aaaaagtcga gaaacaagca gcagatatgc caacaagcag aggaaatttc cagggaaaag
     7981 gaggattccg ttccggattt tccagaggag acggacctcg cagcgtgcgt cgctcggaca
     8041 atccggatgt gctttacgga cgtgactttg aaagtgaatc tctggcaatc gagcagatcc
     8101 agggagagat gggtgaagtg acgattcgcg gacaggtaca gacgctggag acgcgtgaca
     8161 tccgtaataa taaaacaatt attatgatgg aagtgaccga ctttacagat accattgtga
     8221 tcaagatttt tgcacagaca gagcaggtgc cggagatttt aaaagatatc aaaaccggtg
     8281 cgtttctgaa aatccgcggc gtgacgacaa tcgaccggtt tgacggacag ctcagtatcg
     8341 gttcggtgtt tggtatcaaa aaaatccagg atttccgcaa acagagggtg gacacctggc
     8401 cggagaaacg tgtggaactg cactgtcaca caaaaatgag tgatatggat ggtgtttccg
     8461 atgtaaaaga catcataaac cgggcaaaat cctggggaca taaggcgatc gccatcacag
     8521 atcacggtgt tgtgcaggct ttcccggatg cgaaccacgc ggtttcgccg gatgaggact
     8581 ttaagattat ttacggatgt gaggcttatc tggtggatga cctgcaggac ctcgttgtca
     8641 attccagaaa tcaaagcctg gaggatgcct atgtagtctt cgacctggag acaaccggtt
     8701 tcagtacggt ggcaaacaag atcatcgaga tcggtgccgt caaagtggta aatggtaaga
     8761 tcacggataa attcagcacc tttgtcaacc cggaagtgcc gatcccgtat cggatcgagg
     8821 agctgaccag catcaacgac aacatggtgc tcgacgctcc gacaatcgaa gaaatcctgc
     8881 cgaagttcct ggaattttcg gaaggatgcg tgatggtcgc acacaatgca tcgtttgaca
     8941 tgggatttat cgaagcaaac tgcaaacggc agggcattga aaaagaattt acggttgtcg
     9001 ataccgtagc acttgcaaga gtgttgatgc cgcagctgaa ccgttttaag ctggatacgg
     9061 ttgcaaaggc actgaaagta tcactggaga accatcatcg agccgttgat gatgccggat
     9121 gtacggcaga gatttttgtg aaatttattg aaatgcttca gcagagagat attacaacgc
     9181 tggatggagt caatgagctg gggcgtactt ccgtggataa gatccgcaag agtccgtcgc
     9241 accatgcaat tttgctggcg acaaacgatc ttggacgtat caatctctac cgcctggttt
     9301 cctgttcaca tttgaaatat tataaccgaa gaccgctgat cccgaagagc gaatttgtgc
     9361 gtcacagaga gggactattg cttggttctg cctgtgaagc gggcgaactg tatcaggctg
     9421 tgttaaacca tcgctcggat caggagatcg caaggctggt caatttctat gattatctgg
     9481 agatccagcc gatcggcaac aatgctttta tgcttcgaag cgacaagacc gatgtggaga
     9541 cgatcgaaga tcttcaggag atcaaccgca agatcgtaaa actcggagaa cagttcaaca
     9601 aaatggtcgt ggcgacctgc gatgtgcatt tcttaaatcc ggaggatgaa gtttaccgcc
     9661 gcattattat ggcgggcaaa ggatttaccg atgccgacga acaggcaccg ctttacctgc
     9721 gtacaacaga agagatgctc aaagagtttg aatatcttgg cagtgaaaaa gcagaagagg
     9781 tcgtgatcac aaacccgaac aagatcgccg atatgtgcga aaaaatatcg ccggtaagac
     9841 cagataaatg tccgccggtc atcccggatt cggacaaaag tctgcgtgag atctgctaca
     9901 accgtgccca tgagatttat ggcgaggatc ttccggaagt cgttgtcgag cgactggaga
     9961 gagagttgaa ctccatcatt tccaatgggt ttgccgttat gtacatcatc gcacagaaac
    10021 tggtgtggaa gt
//
LOCUS       DAWDWH010000057         9965 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3430_length_9965_cov_12.927952, whole genome shotgun sequence.
ACCESSION   DAWDWH010000057 DAWDWH010000000
VERSION     DAWDWH010000057.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9965)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9965)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9965
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3430_length_9965_cov_12.927952"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2775 a         2235 c         2098 g         2857 t
ORIGIN      
        1 gttttttttc aggaaatgta gtaaaataga aaggaatgcc aaaaagaagc tgaaaggagc
       61 ttaacttgtg aaaaatttaa ttgtaggaca gtccggcgga ccgaccgccg ttatcaacag
      121 cagtctttac ggtgtgatcc gtgaaggttt taaccgcagc gaagagattg aacatgtcta
      181 cggcatgatc aacggcatcc agggattttt agacggacgc tttatggata tgaaagaact
      241 ggataacgat acactggaag ctctgcgtac aactccaggg gcttaccttg gttcctgccg
      301 tttcaaactt ccggatgatt taaatgatgc gatctatcct ctgctgtttc agaaatttga
      361 agagatgaac atcggttact tcttttatat aggaggaaac gattctatgg atacggtcag
      421 caagctttcc cgctatgctg ctgcccacaa cattgatgta cgtatcatgg gtattccgaa
      481 aacgatcgac aacgacctga tcctcaccga ccacacaccg ggattcggca gtgccgcaaa
      541 atatgtggct gcttccgtct gtgaaatcgg actggacgct tctgtttata atacaaaatc
      601 tgttactatc attgagatca tgggccgcca tgaccgcagc atccgtactt gcccgcaaat
      661 tcgagcagga caacccggtg ctgatttatc tgccggaggc ggattttgac caggaatctt
      721 tccttgcttc tctggaagaa tgttttaaga caaaaaacaa cctgatcgtc tgtgtatccg
      781 aaggcattca tgacaaggat ggcaccttta tctgtgaata tgcagcctct gcaggtgtcg
      841 atgcatttgg acacaaaatg ctgaccggct gcggcaaata tctggaaaat ctggtgcgtg
      901 accgcctcgg tgtaaaagtt cgctcggtcg agctgaatgt caatcagaga tgttctgcct
      961 tcctgcagtc tgaaacggat gcactggaag ctgcacaggc cggagctttc ggcgtgactt
     1021 ctgccatcag cggtgaaacc ggcaagatgg tcagctttgt ccgtaaatct gacaaaccat
     1081 acgtgatgga atgtggcctt gaggatgtca acctgatctg caatcaggaa aagacggttc
     1141 ctgttgaatg gatcacaaaa gacggaactg atattgctca ggcatttatt gattatgcac
     1201 ttccgctgat tcagggatct ccgaaacttc ctatgaaaga cggactggct gtgtacgcat
     1261 accgcaagcc tgagaaataa cctccacctc tccaaaacag tccgtccaag tctatctcac
     1321 atgagacttg gcacacgttt acaaacactt ccggcaatga cagagacgta agtcatctca
     1381 catctgatac aaaaacagcc gtaacctttc gaaaacaatt tttgtttcga aaggttacgg
     1441 ccttttcgtt ttaaagcaaa tatttacagc ctttttcgct gcttattgat ctgcgttccg
     1501 tattttttaa tatgctttgc cccagtatac catttttttc gccggttttc cgcagcatac
     1561 gcatacgtcg gaaatggttt cctgttcaaa cggcatacag cgtgatgtga ttcctgcatc
     1621 ttcctttaat ttatcttcac attcacgttc accgcaccac atagccttga taaatcctgg
     1681 tttgttgtct gcgatgtctt ttaactcttc catgttatga gcaacataag tatgtgcatc
     1741 tctgtgtttg cgtgctctct ccagcatatc tttctgcatg ctatccagga tcttcggcaa
     1801 ttctgctgcc agttcgtcga ttgctacggt gatcttttca cgtgtgtcac ggcgtacgac
     1861 aacagcctga cctgcttcga tatcttttgg tcccatctcc acacgaaccg gaataccacg
     1921 catctcctgc tcggagaatt tccatcccgg acttctgtca gtatcatctg ttttcactct
     1981 taatccagcc gctttcagtg ttgcttccac ttctgctgct ttctccagga tgcctgcctt
     2041 tttctgctgg atcgggatga tatctacctg taccggagcg attctcggcg gcagcaccag
     2101 accgctgtta tctccatgta ccatgatgat cgcaccgatc atacgtgtgg tcatacccca
     2161 ggatgtctgg tgaacatatt tcagtttgtt gtcacggtct gtaaactgga tatcataagc
     2221 ttttgcaaat ccatcaccga aattgtggct tgttccggac tgcagtgctt ttccgtcgtg
     2281 catcagggat tcaatcgtgt aagttgcttc tgctccggca aatttttctt tgtcggtctt
     2341 tcttcctttt acaaccggga tcgccagaac ttcttcacag aaatctgcat aaacattcag
     2401 catctggatc gtacgtgctt ctgcttcttc tgctgttgcg tgtgcggtat gtccttcctg
     2461 ccataagaac tctctggaac gcaaaaacgg tcttgttgtc ttctcccaac gcataacaga
     2521 acaccactga ttatatactt tcggcagatc acggtaagaa tgaatatctc tggagtagaa
     2581 atcgcagaac agtgtctcag aagttggacg tacacacata cgctcctgaa gcggctccag
     2641 tcctccgtgt gttacccatg ctacttccgg tgcaaaacct tctacatgat ctttttcttt
     2701 ctgaagcaga ctttccggga tcagcatcgg catgtataca ttctgtacgc cggtctcttt
     2761 gaatctgcga tccagctcat gctggatatt ctcccagatt gcatagcctg ccggtttgat
     2821 cgccatacat ccttttacgc tggtataatc gatcagctct gcttttttta ccacatcggt
     2881 ataccactgt gcaaaatctt catccataga tgtgattgct gaaaatatta aagccagaag
     2941 atgccataca aatcgtttca tacatggtac tctcacaatt ctcctctttg gcacctaata
     3001 aaaaagcccc ggtctctgcg taaacgcagg aaccaaggac ttgtcatctc ttcggcggta
     3061 ccaccctgat tagcactgtt aaaaacagta ctcactcatt ttccgttatc gccggaacta
     3121 cgctgtattt tcatacagga ctccaaggca ggttccatct cttccgcaca ggaatctcgc
     3181 accagtgatc ccctctctgt agattgtccg aaatgtacta tcccttttct tcgtcttgcc
     3241 cctatgacga gctgtcagct ctgtttcatt catcagttta gggcaaaatc ctgcgtttgt
     3301 caataagaaa cggcaatttt tcatttcttg ctttttgtcc gggtgtatat taaaatactg
     3361 atgattcaag ggacaaatgg acacaaaacg gatgcttgca tacgtatttg tgtctatgag
     3421 gaccgccaaa acatgaggaa gcagctattt acgcagtaaa tatgcgaatt ccgaatgttt
     3481 tcgtgatttt gagaatacaa aatacggaaa aatcacgtat ggctcatgga tatagacaaa
     3541 ccacagggga ggatacctat gaataaaaaa gagatttctg agttaaagaa gcaattcaca
     3601 ccggacaact gtacgatttc ccgaatctgc ggctgttatg tcgattcgga aaaggaaaaa
     3661 cgtctggaga tgcgtgaatc atttctttcg ctttctgatg aagaaatgtt taaatatttt
     3721 gaaattttca agaagacact ttccggaacg atcggcaaaa atctgctgaa tctggaattt
     3781 ccactgaaag cagaggcgga agacggcgag caggaatttc tgctgcgttt aaaaaacagc
     3841 cgtctgacgg atgatgctct tgttgatgaa tgttatgaca agatcatcga acattattac
     3901 aatgcggaaa attattttat cattctcgta catgctgcct acgatgttcc tggaaaggcc
     3961 aaagataata cagagatgtt cgattcttcc gatgaggttt acgaatacat cctgtgctgc
     4021 atctgcccgg tcaggctctc caaagccgga ctgtgctaca acgaagcgga aaaccgcatc
     4081 cgtgaccgta tccgtgactg gatcgtggaa cttccggaac gcggtttcct gttcccggca
     4141 tttaatgatc gcagcaccga cctgcatgcg atgctctact attcaaaaaa gccggaagaa
     4201 cttcaggcag aatttgtgga aaatatgttt ggctgtgaag cttcccttac tgccggggaa
     4261 cagaaggaat cattccaggc tgttatttcc gacactctcg gtgaagaatg taattatgaa
     4321 acagtcaaaa atattcatga caatctgact atgctgatgg aggaacacaa agatgatccg
     4381 gaaccggtca tgctggataa aagggaagta aagcagcttc tggagcgaag cggtgtggaa
     4441 gatgaaaagc tttctgattt tgatgctgcc tacgacaatg cagtcggtga agatacttcc
     4501 cttatggctt ccaaccttgt aaacaccaga cgttttgaga tcaaaacccc tctgatcacg
     4561 attcaggtca gcccagagta tgctgatctt gtggagacac gtattgtaga tggcaaaaaa
     4621 tgtcttgtta tcacggtgga tgataatgta accgtcaacg gaattccggc caaaacaatg
     4681 gatcttgatg aataaatgct ccggatcagg tatcgtttct gtttgattac gattctgaac
     4741 taaaaatgga ttctgcttta tttttataat tttttaacaa tttttactgt tttttcaaag
     4801 caaattacgt ttattttaga tattgcattt ttcatttttc tttcttataa ttttatccaa
     4861 caagagacga aggtgtcaaa gctgaatgga ttttcagttt tgatgccttt tttgtttttc
     4921 gggatgtgca caccgggaaa ataagcaaag gaggttattt ttatgaattt ttcatctgtt
     4981 gatgtcatct ggacattact tggagccgca cttgtttatt ttatgcaggc tggttttgca
     5041 atgtgtgagg ccggacttac gcgtgccaaa aataccggca atattcttat gaagaatatg
     5101 atggatttct gtatcggaac tcccgcattc tggctggtcg gattcggcct gatgttcggc
     5161 ggaagcggtg cttttatcgg aaaactcgat ccgctaatcc gcggaagtta tcagtttgga
     5221 tctctgccga cctggtgttt cgtcattttt cagacagttt tctgtgcaac atctgccacg
     5281 atcgtttccg gttccatggc agagcggaca aatttcaaag cttactgttt atatagtgcc
     5341 atgatctctc tgatcgtcta tccgatctcc ggtcactgga tctggggcgg cggctggctt
     5401 tctcagcttg gcttccatga ttttgccgga agtactgccg tacatatggt cggcggtgtc
     5461 accgcatgca tcggtgcaaa aatccttggt cctcgtatcg gaaaatatga cagggacgga
     5521 aaaccaaaag caatccccgg tcataatctc accgttgccg cactcggtgt tttcattctc
     5581 tggttctgct ggttcggatt taacggagca agcacaactg caatgtcaac tgacgccgat
     5641 cttacaaaag ccagtctggt atacatgaac acaaaccttg ccgctgctat cgcaacctgt
     5701 actgccatga tctttacctg ggttcgttac ggtaagccgg atgtttccat gacattaaac
     5761 ggtgcccttg ccggtctggt tgcgatcaca gccggatgtg atcaggtttc ccctgtaggt
     5821 gcattcttta ttggtttatt tgcaggtatc ctgctgtgtg tatccgttga tttctttgac
     5881 cgcatcgcaa agattgatga tccggtcggt gccatttccg tacacggtgc ctgcggtgcc
     5941 atgggaacac tctgtgttgg cctgtttgca acagacggcg gtctgtttta cggcggaggt
     6001 atccattatt tcctgatcca gctgctcggt gttgcctctg ttatgctctg ggttatcatt
     6061 accatgacca ttattttcaa agtcatcgac aaacttgttg gtctgcgtgt tccggctgat
     6121 atcgaaatcg agggcctgga ctatcacgag catggcctgg caagtgctta tgccggattc
     6181 aacatttctg atattactgc tacgatggat gtgaatgaaa atacagatct tggtgaatct
     6241 gattatacga aagcaagcga ttcacagaaa aatgccgccg tcaaagtagc cggtgaagaa
     6301 ttggttgctg ccaatccaac cggcatgtac aaagtttcca tccttgtaaa actttcccgg
     6361 tatgacaaac tccgcaaagc tctcaatgaa cttggtgtca ccggtatgac cgttacacag
     6421 gttatgggct gcggtatcca gaaaggttcc ggcgaaaaat accgtggtgc cgaagttgac
     6481 gctacactcc tcccaaaagt taaagtagaa gtcatcggcg gcaatatccc ggtggaaaaa
     6541 gtcatcgaaa ctgccaaaca atgtctctat accggtcaca tcggcgatgg caagatcttt
     6601 gtatatgacg tacggcatgt tgtaaaagtc cgtaccggcg aagaagatct ggctgcactg
     6661 aaagatgtag aataaaagat gtaagatatt gatttgagaa aagtcggaaa gttcatttat
     6721 ctgttctttc cggctttttg tctttttgag aagctttttc atcttgctat ttttcgtttg
     6781 acagttttta ttttttatag tattctccag attgtcagat tgatttcgat cacctggcgt
     6841 atattgtaca attaaatttt cgatgcatga aatattcatt ttcggatatt tatgcatgaa
     6901 aacctggcag gggatcctcc aatgacgtat ctatgaaaga tgcattattg agtgatctct
     6961 ttttttgttc aaaaatcagt gcacagattt ttatacattc attttcttaa tgtaagtgtc
     7021 aaaagtttaa attgtgattg cacaaatgaa tactgcagac ttattgacac gccgaacact
     7081 tcaaactgaa aaaggagatt attatcagac atgaacacga aaaatttatc aactaaattc
     7141 ctggctgctt ctctggccgc attgatatct gttccgcaga ttccggtttc cgtatctgct
     7201 gccgatctga aatctgtttc catcgccgga aacacctatt cttattctgc ggaagctttg
     7261 gatggatata cgcagatcgg aaacagtggg gtatattatg ctttaacaga cggaacaaaa
     7321 ctttccggaa atttatacgg aacctccaat ctgacctaca aagaattcta ctccggtgat
     7381 gtaagcagta cagacaactt cgatgttgtc acaactgcca caaccttaaa agccaaagtt
     7441 ctgtcaaatg cctggacaga ttataatgaa gaaactactc cggatgctga cggctaccat
     7501 atcctgggag tctccaacgt aaatgttgca gtagattctg atctgtatgt agaagcttct
     7561 attctgaaaa attcgggcaa agaactgacc ggtgcatatg aagaagcttc tgatattaca
     7621 ctgaatgaaa atcctgcaga ggcacctgca cagtacaaaa ccctgaaaag tgacggaagt
     7681 tatgtttcca acgctaaaat catcgatacc gtaacagatg ccactgcttc tctggcaacc
     7741 gcatctacct ggggcgagta cgaaatcgaa gtaaaagaaa acagcaccaa atatttaaga
     7801 aattcaagag aagacacctt ccctgtcggt tccacgatcc agggaatcat tctggaaacg
     7861 acagacggct ataaagtagg tctggaacat ctctccaata tctgggttca gccttacaaa
     7921 ttagctttta atgtaaacaa cgaagctgca acgatgggaa tcgcaaaatc agataatatt
     7981 gcagaattta aaaaactggt aaacaagacc atccagaaaa tcacttatct gacaccggat
     8041 ggaaattatg tttacgcttt tactgacggt gcctttgtaa aacctgcata cgctgaggaa
     8101 atcagcggta ccttcagcag tgatatgaaa acctttactt taaatcaggt tccaaccgtg
     8161 aaaaacggaa ccttaaacgt cctttacacg gtaggatccg gacgtaagaa aacttcttac
     8221 actttatatt ccgatgctgt tcagaaaacc gtttttcttg attttagcag tattcctgct
     8281 gatgcagaag ccggaacata cagcgtatcc attgcttctg atgattatgc agatatttct
     8341 gttgcaatcc cgatcaccga tcaacagaaa gaacagctgt cctctctgct caaacaggca
     8401 gaagctgctt tagccaaagc tgctgcaaat gatcaggtat tagctgcaca caaggatgaa
     8461 gctgtcgaac tgttaaaatc tgcatccgct accagtgcgg atgccgctga tctgatcaac
     8521 gaacttacgg aactgttaaa accgtaccag aataccgaaa caccggatac aggcgataaa
     8581 acaccggaaa ctcagccttc cgaaagcaat cagaccccag agacacagcc ttccaatacc
     8641 actccttcaa cggacaaaac ctcatcactg gcaaaacaga cggtaaaatt aaaagcattc
     8701 ggcaaaggaa agctgaaagt atcctggaaa aaagataaaa acgcttccgg ttatgaaatc
     8761 acatattcta caaaaaaatc attcaaaaac aagaaaaccg ttgttgtaaa gagcaaaaaa
     8821 acaaccagca aagtgatcaa aaagctgaaa tccaaaaaga catattatgt caaagtccgc
     8881 tcttacaaaa aaagcggaaa aacaaaagtt tacggctcct acagcaaagt gaaatccttg
     8941 cgttcaaaat aaaagcagtt tgagacgaag caaattctga caataaaaaa ggatatagcg
     9001 aacattgaat tgtactttca atattcctgt atccttttcc tggttcatta ttattggtga
     9061 acagcgtccc atggcatcta cgctttcctg cgtaaattca tggaatacgc cttgcccatc
     9121 atactttccg ctttgatata catggaatcc gtcaatatcc atacgcaact ctttataagt
     9181 aagcagagat tgattgtgat tttccgcagt cctgcggcga agaatcgctg caatcttgca
     9241 taacaggatt tttggcgaaa agggctttgg aatatagtca tcaatctgct gttcaaaccc
     9301 ccgtaactgg ttttcttcat ccgaaagtgc agtaagcata atgacaggga cttgtgacct
     9361 tttgcgaatg acctcacata caccatagcc gtcgatttta gggagcataa tgtctaacag
     9421 cacaaggtca atggtatcat caaacattgc aatccccgcc acgccgtcgg aagcgatggc
     9481 aacttcatat cctgcatcca tcagatggtt tttcaatata tcctgaatat caatatcatc
     9541 ttcgacaatt gaaactttct gcatcattcc acctccggtt tatagaataa catagttttt
     9601 tgaatttctg atgaagtgca ttatgattct tatagattgc cataatgctt catcctataa
     9661 atacttttta acagcactct ttgtttctat cgttgcaata atcttctttt ccgggtataa
     9721 taaggaaaaa cattctacac ctcaaaggag agatttcaat gatagaacag ctcattgccg
     9781 aagcaactga atgtgatttc aaagttgctc tcgaaacgaa aaagccaaaa agctggttaa
     9841 aaagcgtcag tgccttttcc aacgtaatcg gcggaactct gttttttggg atttctgatg
     9901 accggaaaac aatcggcttg tccgatgttc aaaaggatgc tgaggcgatc agccgcttaa
     9961 tcaaa
//
LOCUS       DAWDWH010000058         9952 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3439_length_9952_cov_13.413762, whole genome shotgun sequence.
ACCESSION   DAWDWH010000058 DAWDWH010000000
VERSION     DAWDWH010000058.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9952)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9952)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9952
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3439_length_9952_cov_13.413762"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    908..917
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7838..7847
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3032 a         1978 c         2411 g         2511 t
ORIGIN      
        1 acacaagatt ttttattaaa atataataaa gataaaaaat ctgtaaagtg aagttttaga
       61 aggaggaaaa atattatgaa aaaattcaca agattttttg ctaccgttgc agcagtctct
      121 atggtagctg cttctttggc aggatgtgga agtaaatctg acaacacagc aagcacagac
      181 acaactgctg attcagcaac agcaggtgat acactgaaga ttggcggaat cggaccgatc
      241 accggaggag cagccgtata tggactggct gttaaaaatg cagcagagct ggcagtaaaa
      301 gagatcaacg aagcaggcgg tatcaacggc atgcaggttg aatttaaatt tgaagatgat
      361 gagcatgatg cagagaaatc ggtaaacgct tacaactccc tgaaagactg gggtatgcag
      421 atgctgatgg gtactgttac atcaacaccg tgtacagcag ttgttgagaa aacacatgaa
      481 gacaatatgt tccagctgac accgtcaggt tccgctgtag aatctatcaa atatgacaat
      541 gcattccgtg tatgcttttc tgacccgggt cagggtactg catctgcaaa atacatcggt
      601 gagaataagc tggcatcaaa agtagccgtt atctacaaca gctctgatgt atattcttcc
      661 ggtatttatc agaaatttgc agaagaggca aaaaatcagg acttcgaagt tgtttctgct
      721 gaagcattta ccgaagacag caagacagac ttctcagtac agctgcagaa agcaaaggat
      781 gcaggtgctg acatggtatt cctgccgatc tactatacag aagcttctct gatcctgaca
      841 caggcaaact ccatgggtta tgctccgaca ttcttcggat gtgacggtct ggatggtctg
      901 ctggcagnnn nnnnnnnagt taagaacttc gatacaaaac tggctgatgg cgttatgctt
      961 ctgacaccgt ttgcagctga tgcagatgat gaaaagacac aggcatttgt ttctgcttac
     1021 aaagaagctt acggcgatac accgaaccag ttcgcagcgg atacttacga tgcaatctat
     1081 attatgaaag cagcagcaga aaaagcaggt gtgacagctg atatgagcgt aagcgacatc
     1141 tgtgatgcaa tgaaaactgc catgacagag atcagctacg atggtctgac cggtgaaggt
     1201 atgacctgga cagcagacgg tgagccggat aaggcaccaa aagctgtcgt gatcaaagac
     1261 ggtgtttaca cagcaatgta atgatgccgg tgtcgattgt gggagtgcga agcacggaac
     1321 atccacaggc atctcaggtg gggatttttg ccccccaaca tcatcgtaat aaaaaatgaa
     1381 gaggcactta cagttcggta aggaagccca gaggagggct gccatgggca gccctttttc
     1441 ctgttataag cgaatcctat tgaataagta ggcaaacaca gaaaacagcc atacacagat
     1501 atcaaaggta gacttgtaaa gtctgttttt gatatctgtg tggtattctt atttttaaga
     1561 aaacgatggt tatattaaaa atacgacaag gggtgtgcgt atgagtttta tttcttatct
     1621 gatcaacggt atcagccttg gaagtgtcta tgcaatcata gcacttggct atacaatggt
     1681 atacggtatc gcaaagatgc tgaattttgc acatggtgat atcatcatgg taggcggtta
     1741 tgcagctttt atctccatga attctttagg cgtgaatcca attctggcag ttatattatc
     1801 ggttgtcatc tgtacgattc tcggactggt taatgagcgt gttgcttacc gtccgcttcg
     1861 aaatgcatct tcaccgctgg ctgttctgat cacagcgatc ggtgtcagct atctgcttca
     1921 gaacctcgca cttctgatgt tcggtgcaga tacaaaatct tttacatctg ttgtaacagt
     1981 tccggcagtt gtgctggcag gcggaaaact gacgatctcc ggtgagacga tcgttactgt
     2041 tcttgcatgt atgatcatca tgatcggtct gacactgttc atcaacaaga caaaagcagg
     2101 tcaggcaatg ctggcagttt cggaggatcg aggtgctgca cagctgatgg gcatcaatgt
     2161 aaatggaacg attgccctta cctttgcaat cggttctgca ctggcagcaa tcgcagcggt
     2221 tctgctgtgt tctgcttatc cttctctgac accgtacacc ggagccatgc ctggtatcaa
     2281 agcattcgta gccgctgtat ttggcggtat cggatcgatt ccgggagcac tgatcggagg
     2341 tattttgctt ggtattattg aaattctcgg caaagcatat atttcctctc agatggcaga
     2401 tgcgatcgta ttcggagttc tgatcgttgt acttctggta aaaccaagcg gattgcttgg
     2461 aaagcagatt caggagaaag tgtaggtggc agcgatgaaa aaattaaaat taaacaaaac
     2521 gacaaaaaac aatctgatca catacggaat cgtcatcctc gcttatatca tcgtagaagc
     2581 aatgctggca ggaggtcaga tctccagtct gatgaaggga cttttagttc cgctctgtgt
     2641 ttatgtgatt ctggctgtat ctttgaatct tgtggtaggt attttaggtg agctgagtct
     2701 tggacatgca ggatttatgt gtgtgggtgc attctccagt gcattcttct caaaatgcac
     2761 cgcaggaatg atgccggatg gacttcgttt ctttctggca ctgctgatcg gagctgctgt
     2821 tgcggctctg ttcggactga ttatcggaat cccggtactg cgactgaaag gtgactacct
     2881 ggcgatcgtt accctggcat ttggtgagat catcaagaac ctgatcaacg tattctttat
     2941 cggaaaagat gcaaatggtt tccatttttc tatgaaggat gccatgtcgc tgaacatgga
     3001 cggaggagat gttatcatta acggaccaca gggaatcacc ggaacgccaa atgattctac
     3061 gtttaccatc ggcgtgatcc tggtactgat cacactggtt atcgtactgc atctgatcaa
     3121 ttccagagac ggacgtgcga tcatgtctat ccgtgacaac cgtatcgcag ctgagtctgt
     3181 cggtatcaat attacaaaat acaaactgat ggcatttatt atttcagcag cccttgccgg
     3241 agttgccggt gttctgtatg cacataacct ggctaccctg actgcactgc cgaaaaactt
     3301 tggttataac cagtcgatca tgatcctggt atttgttgta cttggcggta tcggaagcac
     3361 aagaggttct atgatcgcag ccgttgtact gacactgctt ccggagcttc tgcgcggact
     3421 gaatgactac cgtatgctga tctatgcaat cgttctgatc gtgatgatgc ttctgaactg
     3481 gagcccgaaa gcactggaaa tgagagaacg ttattcaccg aagcgtctgc ttgcaaagag
     3541 aaaagaagca aaggaggcga aataatatgg agaacatgtt agaggtaaag aatctgagca
     3601 tttcattcgg aggattgcgt gccgtatccg aatttgatat gaatatcgga aaaggtcagc
     3661 tctatggcct gatcggaccg aacggtgccg gaaaaacaac catatttaac ttgctgacag
     3721 gtgtttacaa accggatgaa ggtattgtaa aattagatgg acaggacatt accggaaaga
     3781 agaccattga tatcaacaaa gcaggtatcg cccgtacttt ccagaatatc cgcctgttct
     3841 cacagatgtc cgtactggac aatgtaaaag taggactgca caatcatcat cactacaata
     3901 cgctcgaagg gattctgcgt ctgccgcatt accgtaaggt ggaaaaagag atgaatgaac
     3961 gtgcgatgga gcttttgaaa gtgttcgacc ttgagaagga tgccgagtat ctggcttcta
     4021 atctgccata tggacagcag agaaaactgg agatcgccag agcacttgca actgatccga
     4081 agcttctgct tctggatgag ccggcagccg gcatgaatcc gaacgagaca aaagaactga
     4141 tggaaacgat cagctttgtg cgtgatcatt ttaatatgac aatccttctg atcgaacacg
     4201 acatgaaact ggtttccggt atctgtgaag agctgaccgt actgaatttc ggaaaagtac
     4261 tggcacaggg accgactgcg gaagtactga atgatccgca ggttatcaca gcatatctgg
     4321 gagaatagga ggaaatggac ttatggcaat gcttgaggta aaagatctga atgtatacta
     4381 cggtatgatt caggcgatca aggggatttc ctttgaagta aatcagggag aagtcatcgc
     4441 actgatcggt gccaacggag ccggaaagac tacaacactt catgcagtca cagggctgac
     4501 accggtaaaa tcaggccata ttttcttcga aggaaaagat attacaaaag tgccggctca
     4561 caagatcgtt tctatgggaa tggcacacgt tccggaagga cgacgcgtat ttgcacaact
     4621 cagtgtttat cagaacctga aaatgggtgc ttatacacga aaagacaaag acgagatcag
     4681 gcagacactt gatatggtat atgagcattt cccgcgtctg gaagaacgta agaaccagat
     4741 ggcaggtacg ctttccggtg gtgaacagca gatgcttgca atgggacgtg cactgatgtc
     4801 tcatccgaag atcatcctta tggatgaacc gtctatggga ctttcaccga tttttgtaaa
     4861 tgagattttc gaaattatca agaaagtcag tgcagccggc acgaccgtac ttctggtaga
     4921 gcagaatgca aagaaagcac tctccatcgc agacagagca tatgtcctgg aaacaggcaa
     4981 aattatctta cagggtgatg cagacaagct gttaaatgat gagtctgtca agaaagcata
     5041 ccttggagaa taaaaggggg tttgtgctat gggaaaactg atcgttacga tcgcaagaca
     5101 gtatggaagc ggtggaaaaa ccatcggtca gatgctggca gaaaatctgg gtgtaaactg
     5161 ttataacaga gaaattctgc gtatggcatc cgatgacagc ggaatcaaag aagagctgtt
     5221 caatcaggca gatgaaaaac ttcgcaataa tccgctcttt ggaaattcca caagaatcta
     5281 caaaggcggg ctgatccagc cggatcatgg cggatttgtc tccgcagaca atctctttaa
     5341 ttatcaggca aaagtgatca aagaactggc agagaaagag tcctgtgtat ttatcggacg
     5401 ctgtgcagac tttgttctca aagaccgccc ggatgtgatg agcgtcttta tccatgcacc
     5461 gcaggattac tgcctggcac gtggtatgga acgcaactcc atgacggaaa aagagatgga
     5521 aagatttata gcaaagacag acaagtatcg aggtgatttc tatcattatt ataccggacg
     5581 cacctggaac gatgcgagaa attacgatct gtgtctggac agcagcaagc ttggatttga
     5641 caaatgcgta gaagcaatca aggcatacgc aaaagtaaga tttggcgaag atatttttga
     5701 ataagcgtcc agcaatacaa taagtatcag gagcagatgt gagatacttg cataaataaa
     5761 gcaagaatgc ggcagggaga ggaaactttc tctgtcgttt ttgtctctta gctgaagaaa
     5821 actttttata gcgattctgt caggtaaatg tcagcagagt tgaccagaat tgcatgccag
     5881 atctcatttg cgttcgactt aaaaagaagc aggcaacaaa caggtgtgtt tgagtcaaat
     5941 ttatgtggag aggataatat gacactacga gaagaagcag ataaaatcat aaaagaagcg
     6001 atcacaaaag tactgccgga tgaggcagtg aaacaggcac tgaaaaacag aaaaccagta
     6061 aaaggaaaac tttaccttgt ggcggttgga aaagcagcct ggcagatggc aaatgcagcc
     6121 gcaaacctcc tgaatggaca gattgagaaa gggattgtga tcacgaaata cggacatatt
     6181 cagggagaca ttccggggat cgaatgtttt gaagcaggac atccggttcc ggatacaaat
     6241 acctttgcgg ccacacagaa agtgatcgag ctgacagaag aattgcaggc agacgatcag
     6301 gtactatttc tgctttccgg aggcggctcc gccctttttg aaaaaccatt gataagtaca
     6361 gaagaattat cggatataac aaagcagctg cttgcatgcg gggcagatat tgtagaaatg
     6421 aacaccatac ggaaaagact gtccgcagtc aaaggcggaa aatttgccgg actctgtgaa
     6481 ccggcagcca tatttgcgat tgtactgagc gatatcgtcg gagatccgct tgatatgatc
     6541 gcatccggac cggcatatgc agactcttcg accgcatccg atgcaatcgc aatcgcagaa
     6601 aaatatcact taaaactcag cgagaaggca agaacattac tggagatgaa aacagcaaaa
     6661 gcagtaacaa atgtggaaac caggatcacc ggaagcgtca gaaatctgtg cctggcagca
     6721 aaagaagcct gcgaaaaact tggctacgaa agcatgatcc tcacagatgc cctgcagtgt
     6781 gaggcaaaag aagcaggagc atttttagca gcgatcgcaa aaacacatca aaatacagac
     6841 aaaagccttg cttttatcgc gggaggcgag acgatcgtcc acgtgacagg aaaaggactc
     6901 ggcggacgca accaggagct ggcacttgcg gcggcagaag gactggctgc atgcagaaat
     6961 actgccattt tcagcgtcgg aagcgacgga accgacggac cgaccgatgc agccggtggc
     7021 tattgcgatg aaaatacaaa aagaaaactg cagctggaag gaatggacgt ttatgaagtg
     7081 ctgaaagaaa atgatgccta ccatgcactg aaaaagacag gaggactgat cattaccgga
     7141 gcaaccggga caaatgtgaa cgatgtggca gtggtgctga tacggcggag attcttgtga
     7201 taatcagctt ttcactcggg aaggccccct ttttggtaac cttccgaaaa ctggagttca
     7261 cactttcaat tgggtttgtc ggggtatcgt caaaaatagt gtgtaaaata atacagccat
     7321 gatgaatccg aacaggaaat gcagcgtgtc aggagattgt tttcgaatca tcatagcgtc
     7381 atattttaca tataagtttt cttaatgccc atctttttgc ccttcggata agaagcatcc
     7441 ctcgctctaa agagctcggt caatttttat tttgctactt attgccaatc cattgttttg
     7501 cgatttcgac atttacttgc agtgccgaag caatttgctc aaccggaacg tgaagttctg
     7561 caaaagcaat agcagcatcc ttttttgcag tatcaatgcc gctattaaat ccgttattaa
     7621 atccatcgct gaatccagca gccacaccag cttcatactc ggtttttcgt aatttgcgtt
     7681 cttcctcttc tttgtcgtat tcgaatatac tcatcgagat cacctccgat ttatttgcac
     7741 gaagaaattc ggaaagaatg tcgttacgga tacattcatc aaccgccagc ttaactgctt
     7801 cgttcaaatc caattccttc gcatacttac ggacacannn nnnnnnntgc gcatattctc
     7861 ttaaagtctg gcattgttcc atcagctctt tattaagccc ttcattgatg tttattgtca
     7921 gcacttccag ctccagtttc ggttcgccgg acagattttc gaaagattct gacaaatggt
     7981 ttaaccaatg gtctttcttt ttttcagttc cattataaaa tacaataaac tgtggtgccg
     8041 ggattttcag cagtgtggaa gagtaaagcg atttatgatc gactaacttc tgatattcgc
     8101 tggaaatata gaataaatcc cgcagcggca tatttgggtt ataagtagac tgatgttcat
     8161 atagaaacaa gtcagtacca ataatgaaag ccagatcatt tttcattccc atataaatag
     8221 cattctccaa tgtgacaatc tcaagcattt cagggtcatc gtaatgtgaa ccgctaactg
     8281 cattataaag cgacaataaa ttttgacggt cagaaaacag catacgaaat accgtatcct
     8341 tgtaattacg atttgcagtt gcttgttctt tttgagccat aggcagtacc tccttatgca
     8401 ggagtgtacc aaaatgcgac agccatacgg tttatactct ttttcacagt ttgaaactcc
     8461 tgctttttaa attgatttgt ttgattgaaa agcatgaatt tctgaagtgt gaattagaaa
     8521 atatagatgg caggatgcca agaacgttaa gaaatatgct ttgaaaatat attaccacat
     8581 agctctcatt atttcaatat gttttaaaaa gataattaaa gtcaacataa gcaataagat
     8641 tccaacgact gtatagaaaa gcagatacaa atttttactt tgcaacatag tatagaaaca
     8701 aaatacagaa agcagcattt tatgaatgat cagaagcttg aaaatcttct aaatcttgca
     8761 ctggatgcta cagatgcaga acgggaaaaa tctttaaaca tgaatttcgg gtatgacagg
     8821 cagaatcgga tttgggagct gatcgtgcga tatcacggtg atctttccgg ggttggggag
     8881 cggacaaaag agattatcct gttatccggc ggctatgcga tcattacggc agaagagagc
     8941 gagattgact ggatttcctc gcagccggaa gtcgaataca tcgaaaagcc gaaaagactg
     9001 tttttttcag cagcacaggg cagaagtgcc tcctgtatca atccacttca gaccggggca
     9061 tttgatctga gaggccaggg cgttttggtg gcggtgatcg attccggcgt agactatttt
     9121 catccggatt tccgaaatga ggatggtaca accagaatct tgtcactttg ggatcagaca
     9181 atccaaagac caaaaagaaa tgatgcaatg gagacagata taacaagcaa aggagaaaat
     9241 atatcggaag aagggacaga agacacagga aaaatcaaag aaggaaccgg cgaagaaatc
     9301 cggggtgtgg aatatacaaa agagcagatc aatgaggcac ttgctgccgg aagcagagaa
     9361 gcaggatatc gcatcgttcc atccagagat ctgagcggac acggaacgga agtactcggt
     9421 atcgcagccg gaaatggtca ggcatccggc ggacagtatc gtggtgtggc atcgaagagc
     9481 gatattctgg tcgtgaaaat cggaacagcc ggttcagagt ttgccggaac cgtagaactg
     9541 atgctggcac tggactacgt gatcagaaaa gcgatggaac ttgggaagcc tgttgcgatc
     9601 aacctgagct ttggaaatgt atatggctcg catgtgccat ataccagagg cattcttgga
     9661 ttaaacagaa gttaaaaaac aatcctgcag tttaacaaca attcaatgaa aatggcagaa
     9721 tctgtctgtt acaatacaag taacgaaagc tttcgaatct gaaacggaga aaagactatg
     9781 cagatgaaag aggtcataag atcaaagcga aaagagtgcg gttatacgca agagcaggtt
     9841 acgttctttc gagaaaataa aaattgaccg ttggtttcct gacaggtgta aggaatggaa
     9901 atgccgcaca ttttaaaata gtgtttgcaa aagaatagta ttgcatgtgc aa
//
LOCUS       DAWDWH010000059         9951 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3440_length_9951_cov_12.545675, whole genome shotgun sequence.
ACCESSION   DAWDWH010000059 DAWDWH010000000
VERSION     DAWDWH010000059.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9951)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9951
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3440_length_9951_cov_12.545675"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1561..1570
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3048 a         2017 c         2465 g         2411 t
ORIGIN      
        1 cggtacatcc ctgggatatt ctttcaccga ctttattacc aataccggtc tgatggcagt
       61 cgtatccctg gtatttgtcg ttatctattt ttactttgca tttagaaaag aactgaaaaa
      121 gaacggggca aataaaaaag atcatgcttc ttatccagat ccgaaggaag caatcacaga
      181 caaacgtgga tttatcgcaa gctgtattat tttcctgtgt gctgtcgtgc ttctggtatc
      241 acatgcacag acggggctga ctgtatcgac gatcggtgtt gctattgcaa tcgtaacctt
      301 gatcgtggca ggaaaagatg cactggaact tctgaaaaaa gtagattaca aaacactgct
      361 gttctttgtc ggactgtttg ttgtcgtaag tggtctggag gaaaccggtg ttctggaaat
      421 tctggcaggc tttatcggat ctgtcagtgg cggaaatatt gctgttatga ttgctattat
      481 cattatcgta tctgcagtgg caagtgcttt tatcgataac attccattcg cagcgaccat
      541 gatcccggtt atcacggatc tggcatccga tgtagccggt gtcaacctga ctgttcttgc
      601 atgggcactg gcgatcggta ccgatatcgg aggaagtgca acaccgatcg gtgcatcggc
      661 gaacgttgta ggtatcgcaa ccgcagcaag agaaggtcat attattaaat ggggaaagta
      721 ttgcaaatat atggctcctg ctactataat agtagtagca atatctctgg cgatgatcta
      781 cgtcagatat ttatgatgcc agggtcacgt atgacatcat ttggctttaa cattatacct
      841 ataaaagggg gctgagcaaa atggaaaaac aattaattat ttcggtagga cgtgagttcg
      901 gaagtggcgg tcatgaagtc gcacagaaac ttgctgatga ttatggcatt gcactgtatg
      961 accatgacct gttaaaagag atcgcagcaa agaaaaattt aaaaagtgaa gatctcgaag
     1021 catatgatga actgaaatgg aaccgctttc tgtatcgtac tgttcgcgga ctgaacagtt
     1081 caccggagca gaacgttgca aacctgcagt ttgatttcct gaaagaaaaa gcaaaatcag
     1141 gagagtcttt tgtggtagtc ggacgttgtt ccgagacaat ccttcgtgag tacgaaagca
     1201 tggtatccat ttttatccgt ggtgacaagc cacagaaggc agagcgtatc atgaaatatt
     1261 ataatatgaa cgaaaaagat gctctgggct tcatgaaaga aaaagataaa agaagaaaac
     1321 agtatcacaa ccgttactgc ccgatcgcct ggggagattc cagaaactat gatctggtgg
     1381 taaacagcag ccgtgtcggt gtggacggat gcgtggaaca ggtaaaacat tacatcgact
     1441 tagtaagaaa atatcacaaa taatggattg agccttgcaa cttctctgca atctatgata
     1501 tgatttactc tagtagtgtg aaaattaatg aaacaacagg agggaaatta tgttatcatt
     1561 nnnnnnnnnn ttatcatttt tcaagaaaaa agctgcaaaa tcagaagaag ctgcaaatgg
     1621 caaaactatg aaagcatacc tgaccggaaa agtaattccg attgaggaag taaacgatgg
     1681 tgttttctca gcaaagatca tgggtgacgg tattgcgatc atcccagaaa acgaagttct
     1741 gactgctccg gctgacggtg aaatcaccgt tgttatggac ggatctttcc atgcagtagg
     1801 tatgagactg gacaatggag tagaagtact gcttcacatc ggtgtagata cggtaggcat
     1861 gaacggagaa ggcttcaaac catatgtaac cgtgggtgat aaagtaaaaa caggtgacaa
     1921 cctgatcggt ttcagcaaat ctgcaatcgc agcacatggt ctgcgtgacg tgacaatgct
     1981 gattgttacc aacggcgatg agtgcccacc ggtaaaactg atgtccggta tggatgcagt
     2041 tgcaggcgag accgtagtag cagagatgta atccggacag tattatacaa aaacataata
     2101 tttcttataa aaaacttcag acagagtgaa acctcactct gtctgaagtt tttttagtct
     2161 ccagaacggt gactgtttta ctattagtga tgtcttcgcc agggcacact ttcttcacaa
     2221 aatcacctca gactcatcac atatttacaa catttcaaac acaaatcgca gatatactgt
     2281 tagacataaa gaacaacaaa gaataataaa cattaggttt ccgaaaagga aaaccggatg
     2341 agcaaataca ggaggtagtc attatgtcag atatgtataa cttcgataac aacaattaca
     2401 acaacgagaa tcacgatgga ttcaatcact atgaatttga tgaaaacaac agcaacacac
     2461 cgaaccacaa aaaaccaaat gcaaagatgg taaaatttgc aaagaaagtc ggagccattg
     2521 cattaagtgc cgttctcttc ggaggcgttg cagcaggatc ttatcaggga gtaaatgctc
     2581 tgttcggaac ttccacacag acagaggcac aggcaacaac atcaacaaag agcagtcttc
     2641 tgaaaacaac ttccagcaca acttcaaagg gtacgatgga tgtgaccgac attgtaaaat
     2701 ccgcaatgcc ttctatcgtt tctatcacaa gcaaatcagt tcaggaagtg gaaaattatt
     2761 tcggaatgtt cggcggacgt aattctattc agcagcagga agtcgaaagt gccggttccg
     2821 gtatcatcat cggaaagaat gacagcgaac tgctgatcgc caccaacaat catgttattg
     2881 atggtgcaga gacggtttcg gtatcctttg tagataatga aacttatgag gcaacgatca
     2941 aaggaaccga ttctgataaa gaccttgcag ttgtagccgt tccgctttcc agcatttcaa
     3001 gcgatacgat gtcgaagatt tctgtggcaa cgatcggtga ttcttccaaa ctggaagttg
     3061 gcgaacaagt tgtagccatc ggtaatgcac ttggatatgg tcagtctgtg acaaccggta
     3121 ttgtaagtgc aacaaaccgt acactggatt cggaagacag cagtgaagca agctatgacg
     3181 gtatcagcct gatccagacc gatgcagcca tcaaccctgg caacagcggt ggtgctctgc
     3241 tgaacatgaa cggcgaagtt atcggtatca actccgccaa actggcttcc acagaagtag
     3301 aaggtatggg ttatgcaatt tctatgaacg atgcatatga tacgattgaa gagctgatga
     3361 atgagacgac aagaacaaaa gtctccgatg atgagaaagc aagccttggt atctccggta
     3421 ccggtgtaag tgatgaggca gcagaagctt atggcattcc gtccggtgtc tttgtgtcac
     3481 aggtaaccga aggcggggca gcagagcagg caggtatcac agcaaactcg atcattactg
     3541 catttgacgg caaatccgtc agcgatatca gcgaactgaa aagtcgtctg gaatactata
     3601 aagcaggcga agaagtagaa gtaaccttac aggtaccgga tggcaatggt tatacggaaa
     3661 aaaacgttac cgtcaccctc ggaaaggcgg acagcagcac aagcggaagc agcctgcagt
     3721 ccggcagtga cgaaagtcag gatcagaacc agggacagga tccgtataat tacggcggtt
     3781 ccgatgacaa ttccggaaac tatttccgtt tctcaaacag ccagaaaagc ttttgaacag
     3841 aaaaaatatc ttaaaaattt tcatacagcc aaactacaaa tacaaagttt gcccctgaca
     3901 ttttataacg accctttaca gtacggcttt tcaaatgcta tactgtagag ggttttttga
     3961 cgaaaaggaa catagatgag gagtatactt atgaagaaaa gatttgcgat cattctgaca
     4021 gcggcagctc tttccatgac cggatgcagt ctgcttgggc aggatactta ctatatagat
     4081 cagggaatgg aacagataaa cagcggcaat tatgaggagg cactgaccag tttttcttcc
     4141 gcagagcagg aagggaagca tctcgtggag agttaccgcg gcagcggcat ggcgtatatg
     4201 gcacttggag aatacgacaa ggcggtagag gcgtttgacc gtgcagtgga tctgacaaaa
     4261 gagaagcaga aagatgtgag gaaagatatt ctgatgtaca aggcaacggc attgtatcag
     4321 tccggcgatt acgagggggc ggccaaagtc tgcgatacga tccagacttt cggggacagt
     4381 gcagatgctt attatcttca gggactgtgc tatatggagc tggatgagaa ggataaagca
     4441 ggtgtggact ttacgactgc ggtcaaatta tctccgcagg actacgatct gcttctgaat
     4501 atttatgaat gttatgatga caagaatctt tccgcagagg gggatgctta tcttcagcag
     4561 gctctggcga tcaacagcga cgattctgaa gatatctacc agaaagcgag aatttattat
     4621 tttcttggcg attatgacga tgcaaagaca gagctgaata aaatggcaga cagcatggat
     4681 gagcgttcga ccatgcttct gtgcagaact tatatgaagc aggatgacac cgcacacgca
     4741 aggcagcttt atcagcagta catggacaaa tatggtgaga caccggaggc atacaatggc
     4801 atggtactgt gtgatattgc ggatgaaaat tatgacagtg ccctggaaaa catcacaaaa
     4861 ggcctggcac tttcaaacga caaaggaaaa caggatctgt attttaacga gattgttgtc
     4921 tatgaaaaga agctggattt tgcgaccgca aaagaaaaag cagcagaata tgtgaaaaat
     4981 tatcctgcgg acgaggcggg tatccgggaa aataatttcc tgaagaccag ataattatgc
     5041 agaaggagga tcatttatgc agccatatga catgaaagaa ctggaagaga gggtgatcct
     5101 ggtcggcgtt caggagagtt tttcggaaga tgtggaagaa tctctggatg aacttgcaga
     5161 gctttgtgag acagccgggg cattggtggt cggaagggtg atccagaatc gtgaaaagat
     5221 ccacccgggc acctatgtcg gaaaaggaaa gatcgaggag atcgcccttc tgatggagga
     5281 actgggagcg accggcattg tctgcgatga cgagctttct ccggcacagc ttaataatct
     5341 ggagaaagag ctggactgca aagtcatgga ccgtacgctg atcattctgg atatttttgc
     5401 ggcgagagcc aacacaagcg agggaaatat ccaggttgag cttgcacagc tgcgttaccg
     5461 tgcggcaagg ctggtcggac ttcgcaattc gctctccaga ctcggaggcg gtatcggtac
     5521 gagaggaccg ggtgagaaaa agctggagat ggacagacga ctgatccatg cccgcatttc
     5581 acagctcaag gcagagcttg cacaggtgca gaagcaccgt gagctgataa ggagcaaacg
     5641 aaaaaaagac cagatggtgg ttgcggcgat cgtcggttat accaatgccg gcaagtccac
     5701 gttgctcaac acactgacag atgccggtgt tctggaggaa gataagctct ttgcaaccct
     5761 tgatccgacg acgcgccttc tggaactgcc gggcggtcag aatattcttc tgacggatac
     5821 ggtcgggttt atccgcaaac tgccgcatca tctgatcgag gcctttaaga gcacgctgga
     5881 agaggcaaag tatgcggaca tcatcatcca tgtggtggat gcgtccaatc cgcagaagga
     5941 aaaacagatg catgttgttt atgagacatt aaaagatctc ggtgtgaagg atcagccgat
     6001 cctgacgctg ttcaacaagc aggaccgtat tgaaaacccg gaaattttaa aagacatgaa
     6061 agcggacaaa actttaaaaa tttccgcaaa aacagggcag gggcttgatg atctgaagga
     6121 cgagctggag aagatattgc gtgccagaag catcctttta gagcgtgttt ttccgtatga
     6181 aaaggccggt ctgatccaga ttgtgcgggc aaaaggacag cttctggaag aggagtacag
     6241 gggcgacgga atctacgtaa aagcctttgt tccggcggag atttacggca aagttgcata
     6301 ttaaaaagtc agaaatcaca caaacaaaat actatatctt gtatgtattt ttaaaataca
     6361 atccaagata tagtattttt tgcattgact cgcaaacgat aatctgctag aataagttcc
     6421 tgacagcatc tgccgggagg cggatgcgaa agacgataat catgcggaag gagcagataa
     6481 tatatatgtt taaggtagta aaaagggacg gcgaagtcgc agactttgac cttcagaaaa
     6541 tcgggtgcgc tattgagaaa gcatttgatg cgacacagat gcagtataac aaagacatgg
     6601 tagatctgct gaatttgcga gtgactgctg atttccagaa aaagatcaaa gacgacaaga
     6661 tcgatgtgga gagcattcag gacagcgtgg agaatgtact ggttcaggcc ggctatgcag
     6721 atgtggcaaa ggcgtacatt ctgtaccgta agcagagaga aaaaatccgc aatatgaaat
     6781 ctacgatcct ggactacaaa gagatcgtgg acagttatgt gaaagtagaa gactggcgtg
     6841 taaaagaaaa ttctacggtt acttattccg taggcggtct gatcctgagc aactcgggag
     6901 cggttacggc aaactattgg ctgtctgaga tttacgatga tgaggtcgct catgcacacc
     6961 ggaatgccga tatccatatc catgatctgt cgatgctgac cggatactgt gcaggctggt
     7021 ctttgaaaca gctgatcaca gagggacttg gcggcattga gggcaagatc acatcggcac
     7081 cggccaaaca tctgtccgta ctttgcaatc agatggtcaa tttcctcggc atcatgcaga
     7141 atgaatgggc aggagcacag gcattttctt cttttgacac gtatctggca ccgtttgtca
     7201 aagtggacaa tctggactat ccggaagtca agaaatgcat cgaatctttt gtatacggcg
     7261 taaatacacc gagccgctgg gggacgcagg caccattctc caacatcaca ctggactgga
     7321 cggttccggc tgaccttgcc gagcttccgg cgatcgtggg cggcaagccg caggatttct
     7381 gctataaaga ctgcaaaaaa gagatggata tgatcaaccg tgcctttatt gagacgatga
     7441 tcgagggcga tgcaaacgga cgcggtttcc agtatccgat cccgacttac tcgatcacaa
     7501 aggatttcga ctggtccgat acacagaaca accgcctgtt atttgagatg acatccaaat
     7561 acggtacacc gtatttttca aactatatca acagcgatat ggagccgagc gatgtacgaa
     7621 gtatgtgctg tcgtctgcgt ctggatctgc gtgaactgcg taagaagagc ggcggattct
     7681 tcggttccgg agagagtacc ggaagtgtgg gcgttgtgac gatcaacctt ccgagaatcg
     7741 cttacctgtc cgagacaaaa gaggatttct ataagagact ggatcgtatg atggatattt
     7801 ccgcccgttc cctgaagatc aaacgagatg tgatttccaa actgctggac gaaggactgt
     7861 atccttatac aaaacgttac ctcggaactt ttgcaaatca tttttccacg atcggtatga
     7921 tcggtatgaa cgaagtcgga ctcaatgcaa aatggcttcg caaagacctt actcatgtgg
     7981 agacgcagga ttttgcaaaa gaagtgctga accatatgcg tgacagactg gtgatgtacc
     8041 aggaagagta cggagatctc tacaacctgg aggcaacacc ggcagagtct acgacctacc
     8101 gtctggcaaa acacgacaaa gagcagtatc cggatatcat cacagccggc gagccgggtg
     8161 atacaccgta ctacacaaac agctcccatc ttccggttgg ctatacggcg gacattttcg
     8221 atgcactgga tattcaggac ggacttcaga ctctttatac ctccggaacg gtattccatg
     8281 cattcttagg tgagaagctg ccgaactgga aagccgccgc agcactggta cgcaagatcg
     8341 cagagaatta cagcctgccg tactacacac tatcaccgac ctactctgtc tgtaaagaac
     8401 acggctacct tgcaggcgag catttcacct gcccgatctg cggtaagaaa tccgaagttt
     8461 acagcaggat caccggttac taccgtccgg ttcagaactg gaatgacggc aagacacagg
     8521 agtacaaaaa ccgtaaactt tatgatgtag agcattcctg tatgaaaaaa gtcaagacca
     8581 gccttgtgac tgtacgcaaa gaagatgttg agatcaagcc ggtggagagc gtgaaatacc
     8641 tgttcacgac aaagacctgt ccgaactgca agatcgcaaa agagattctc aaaggagaag
     8701 attatcagat catcgatgca gaggaaaatc aggatcttgt aagaaaatac ggcatcatgc
     8761 aggcaccgac actggtggtg acagacggca caaaagcaaa gaaatacgtg aatgcgtcaa
     8821 acatcaagaa atatgtggca gatgtaaaac agactgtctg attgtgaagt gattcttaat
     8881 ttcaggactc gcagcaagaa tgccagaggc attcttgaaa atcagaaaaa aagggaagcc
     8941 cagattttaa aggacttccc tgatgtctgg cataaaacat aatgaaaaat accgctcctc
     9001 atttgtttat gaatgagaag cggtattttt tggagaatct tcagttcatc cgatcaatta
     9061 gcatttaaca acgttagctg cctgcattcc gcgagcacct tctgttaaat cataagttac
     9121 tgcctgacct tcgtcaagag atttgaagcc ttcaccctgg atagcggaga aatgtacaaa
     9181 tatatcgttt ccgtcttcac ctgtgataaa tccgtaacct ttttcagcgt tgaaccattt
     9241 tactgtacct ttgttcatga tggtacctcc attagataat aaaattttat tgtggacttt
     9301 tgagttaaaa aaatccacca gttattacag attatatagg ggtgagtata atctctaata
     9361 actagtgaat tactttcatc caataaatcc ctcaacggat attatagatt taaccaccgc
     9421 aaatgtcaag agaattgtgt attgttttgc acctgtatat cggttatact gatactgttt
     9481 ccgggcatga atcctgataa aaaaggtagg aaatcctttg tcaggaagcc acaaaacagc
     9541 aaaaagaaag gaagatgaga tgtcagaaat cataaaagcg atcctgttcg gaatcgtgga
     9601 aggaatcaca gaatggcttc cggtcagcag caccggtcat atgatcattc tcgatgaact
     9661 gatcaaactg aacgtgtcaa aagagtttct ggaagtattt ctggtagtga tccagcttgg
     9721 agcgatcctt gcggtcgtaa tgctctactg gaatcagctg tttccgttct cctttaaaaa
     9781 gggcgaagac acgattcgca aagacgtatt ttctatgtgg ttcaaagtaa tcgcagcctg
     9841 cattccggcg gcggtggtcg gtattttgtt tgacgaccag atcaacgagc ttttttacaa
     9901 ttaccagtcg gtagcaacgg cactgatcgt gtttggtatt ttatttattg t
//
LOCUS       DAWDWH010000060         9907 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3458_length_9907_cov_15.037150, whole genome shotgun sequence.
ACCESSION   DAWDWH010000060 DAWDWH010000000
VERSION     DAWDWH010000060.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9907)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9907)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9907
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3458_length_9907_cov_15.037150"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    788..797
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2920 a         1951 c         2655 g         2371 t
ORIGIN      
        1 cggcgttgtc agcggaaaag gcggcgtcgg taaatctatg gtaacggcat ctctggcgaa
       61 cctgatggca gaaaagggct atcgtgtcgg tattctggat gcagatatca caggaccgtc
      121 cattccgaaa atgtatggac tgcacggacc ggctcaggcg gacgataatg gtatttatcc
      181 gccgctggca gaaaatgata tcaaagttat gtctatcaat ctgctgatgc cggatgaaga
      241 agcaccggtt atctggagag gaccggttat ttccggtatg gttaaacagt tctggacaga
      301 tgtgatctgg ggagatctgg actatctgtt cgtggacatg ccaccgggaa ccggagatgt
      361 acctctgacc gtatttcagt cgctgccgct ggatggtatc gtgatcgtat cctcaccgca
      421 ggatcttgtt cagatgatcg taaagaaagc ataccatatg gcaaaaagca tgaatgtgcc
      481 gatccttggt attgtggaaa actacagcta tctggtatgt ccggactgcg gaaagaagat
      541 ttctgtattc ggtgaaagcc atatcgaaga aattgcaggc aaaatggatc ttaaggtact
      601 tggaaagatg ccgatcgatc cgtccctggc agaactttct gataaaggtg attttgcaaa
      661 agcagaaaac aaatatctga cggatgcatt tgaagtactg catcagctgg atcgtgtaaa
      721 aaaagaagag gagtagcgtt ccagggtctt tcacccacct atgagtaagc tcatatggtt
      781 ttagaccnnn nnnnnnncat caagcgggat caaacgaaag ggcagcggca caggaatcgg
      841 gaattgtgtg gaagccggta tctgtcacag cttttcggcg gatggcagat gtatttctct
      901 tttgaaaatt ctgctgacca acgagtgtgt ctatgactgc aagtactgta tcaaccgatg
      961 cagtaacgat gtgccgcgtg ccacatttac gccggaagag gtctgtcagc tgacaatcga
     1021 gttttaccgg cgtaattata tagaaggatt atttttaagt tccggtgtac tgcaaaatcc
     1081 gaactacacg atggagcttc tttatcagac actctgtctg cttcgtacga agtaccattt
     1141 tgaaggatat atccatgtaa aagcgattcc gggggcttcg ccggttctga ttgaaaagac
     1201 cggttatctg gcggacagga tgagtgtcaa tctggagctt ccgacagcgg aaggactgaa
     1261 aaagctggca ccgcacaaga gccgcaggac gatccttgca ccgatgcggc agatccagac
     1321 catgcggcag gaaaatcagt atgatctgac ggtatatcgc cagagttcgc ggtttgtgcc
     1381 ggcaggacag agtacgcaga tgatcatcgg tgcgaccccg gagaatgatc tacagatcat
     1441 ttcggttgcc gagtctctct atcagaagtt tcagattaag agagtctttt attcggcgtt
     1501 tgttcaggta aatgaagatg cagcacttcc gacactgccc ggtggtcctc ctcttttacg
     1561 ggagcacaga ctttatcagg cggactggct gatgcggttt tatggatttg aggcaggtga
     1621 gcttctctca gaaaagcgtc cgaatttcaa tgtattctta gatccgaagt gtgactgggc
     1681 gatcggccat ctggagcagt ttccggtgga agttaccacc gcggattacc atatccttct
     1741 gcgtgtcccg gggatcggag tcaagtccgc aaagcggatc gtccaggcga gaagatacgg
     1801 aagtctttct tttgaaggcc tgaaaaagat gggggttgtt ttaaagagag caatctattt
     1861 tatcacctgc agcggcagga tgtactatcc ggtcaagctg gaagaggatg cgatcgtccg
     1921 taatctgctt gctggagaga agcaggatac ccgtatggaa gtgccgaaat atcatcagat
     1981 gtcgcttttt gatgatacta tgccatttgc ggatctgcca aatatgaggt gacttacact
     2041 tcgtcagaga cgtattacag gagtacaaaa cagagaatta tttacaggac attttttaag
     2101 caaatcaagt aagttccctg cttcaattgc gaaaagcaat aagcagtgtg tggacttgct
     2161 ggtattagga ggatggatag tatgaccgta tttttgtgcg aggacagtgt gtgcgggatc
     2221 ctgaccgggg tttatgatgc gtgggacagt agactgggac atgcaaatat aaaacttgcc
     2281 ctgaaaaaag gatatcagcc cgagcttttt atagactgtc aggaggtggg accggatgca
     2341 gagaaggcag ataaggtact tcgcactctg gatcgaaaaa tgtacctgga agattttgaa
     2401 gctctctatc aggctctcct ctcagcggat gatcaaaaag cagatgacat ctacagaatg
     2461 attgtgctgg cactgcatac cgggaaaaag ctcatccatg cactggagca tccgaccatt
     2521 tttcgcattt tccggatggc gagaaaaacc gggagggagg cagggcactg ttgtgaattt
     2581 ctgagattcc gggagctgga aaaggggatt ctgttttctg tgatcgcacc gcaggcacag
     2641 gttctggcgt tgatcgggga gcattttgca gaccgttttc caatggagca ttttatgatc
     2701 tatgacaaga cgcatcaggc gatgctggtg catgaagcag gaaaggaatg ggtaatctgg
     2761 gaacacatcg aagctttcga tgaaacaaaa ctgaaattgt ccgataacga gaaagaaatg
     2821 agccggggat ggcagatttt ctttgatacg attgcgattc aggaacgaaa aaatgaaaaa
     2881 ttgcagcagc agcttctgcc attaaaatac cgcccatatc agacggagca ccttaaaaaa
     2941 gaagaaaaat gtgcactttt ttccaaatag aagcgttgta tcctgtcaaa atgttgcttt
     3001 ttttgttgtt ttttacataa aaaacgtaaa aaatcacatt gacaaataaa gagcttggtg
     3061 tcataatacg tgcaatgtaa aaacaaagag atttttcttg cagagattgc gggaatgatt
     3121 tcgagacaca acatttcgca gaacaggagg atgcagatta tgttggaaaa gaaaatcaaa
     3181 cttacggaat atgaagaagt caaggaattt gttcatgccg ctgaaaagtg tgattttgac
     3241 attgatgtat tttataacag gataatcatt gatgccaaat ctatgctggg agtgctgagt
     3301 ctggatttgt caagagaatt aaccgtaaaa tatggcggta aaaataacgc ttttgaaaat
     3361 gtgttatgta aatacgcatg tgcataaaga agagcatatg atctgagatc atattccgat
     3421 cataaaaatg ctgcagtacg aagtgagtcc aaaagacaga gctttgtcct gcagcaattt
     3481 ttttgcagaa tcagtatggg agggagtaaa gtggaaaagt ttcggatatc ggtacgttcg
     3541 ctggtggagt ttttgctgca cagcggcgac attgacggcg gaaagggcgg tatggcagat
     3601 aaggaagcca tgcagctcgg aagccggata cacaggaaaa tacagggcag catgggagcc
     3661 ggataccgac cggaagtttc tctgaaggcc gagattgtaa aagaaacgtt tacacttttc
     3721 gtagaaggac gggcagacgg tatcctggaa caggacgggc ggatcacggt ggatgaaata
     3781 aaaggggtgt accgcagtct ggatgctatg gaaaagccgg atctactgca tctggcacag
     3841 gcgaagtgtt atgcggcgat ttacggaagt gagcagaaac tggatgtgat ttcggtgcag
     3901 atgacatact gcaatctgga aacagaagag attcggcgtt ttgtgcagga gttttcagta
     3961 aaagagctga aaagctggtt tgatgaattg acggagcaat actgcaagtg gtgtgaaatg
     4021 caggtgaaat ggaaaacgtg tcggcaggaa agcatccatg cctgtgtttt tccgtttcct
     4081 taccgggaag ggcagaaaca gctggcggca gctgtatacc ggacgatcgc acacaggaag
     4141 aaactcttta tccaggcacc gaccggtgtc ggcaaaacca tatccacggt ctttccaacg
     4201 gtaaaagcag tcggtgaaaa tcttggtgaa aagatttttt atctgacggc gaagacggtc
     4261 acaaggaccg ttgcagaaga agctttttct gttttaaaag gcaaagggct tcgctataaa
     4321 gtactgatgc tgacagcaaa agaaaagatc tgtccactgg aagaggtaaa atgcaatccg
     4381 atcgaatgtc cgtatgcgaa agggcattat gaccgggtga acgaagcagt ttttgaaatg
     4441 ctgaatgaga ccgaggtgtt tgaccgggaa gccatttgga aacagagcga aaaatggaaa
     4501 gtctgcccct atgagatgac gctggatctt tcgatgtggg tggatgccgt gatctgtgac
     4561 tataattatg tatttgatcc gaatgcaagg ctgaagcgtt tttttgggga caatataaaa
     4621 ggagagtatc tgtttctgat cgatgaggca cacaatctgg tggaacgagg acgggaaatg
     4681 tacagtgcaa gtctttgcaa agagcgattt cttgaaattc gccgcctgtt aaaaagcaaa
     4741 agcagaaaaa tcagccgtac gctggaaaaa tgcaaccggg ttcttctgga atggaagcgg
     4801 gaatgcgaag attatcagat acgaaaaaat ataagtgagc tgatcctgcc gctgatgaat
     4861 ctctgtggtg aactggaaga ttttctggaa gaggaaccgg atggagaagt gcatgaaaaa
     4921 ttgctggaat tttattttga agtttctaaa ttcctgaata tttatgatct ggtggatgat
     4981 cattatgtga tctacacgta ctatgcaccg gacggaagtt ttttagtgcg tctctactgt
     5041 gtggatacat cggcaaacat ccagaaatgt ctggacaaag cgaacgcagc tgtctttttc
     5101 tcggcaacgc ttcttccggt gcagtattac atcagtctgc tgagtgaggc gaaggacgat
     5161 tatgcaatct atgccaggtc tccgtttgat ccaaaaaaac gtgctgttct gatcggaacg
     5221 gatgtcagca gccgttatac aagaagaggt caggaagaat atatgaggat cgcagcttat
     5281 atcaaacaga cggtcatgca gaaaaaggga aattatctgg cattttttcc atcctatcgt
     5341 atgcttcagg atgtgatggc gtgtaccggg ccgcagctgg ataccagtat tcgctgtatc
     5401 tgccagacat ccggaatgac tgaggaggaa agagaggcat ttctgcatga atttgagcag
     5461 aagcgaaaag aggacgaggg acttctggga ttctgtgtga tgggcgggat ttttggagaa
     5521 gggatcgacc tgaaagagga cagtctgatc ggagcaatca tcgtcggaac cggtattccg
     5581 caaatctgcc gggaacgtga gattctcaaa cagtattatg atggaagaaa cgaggacggt
     5641 tttgcctatg cctatcgcta tccggggatg aacaaagttc tgcagtccgc gggaagagtg
     5701 atccgcactg cacaggatcg gggcgtagtg ctgctgcttg acgaacgctt tgcgaaaagt
     5761 gaataccgga agatgtttcc gagagaatgg gaggagtatg agtattgcag ccggagtaat
     5821 gtagcagaca aactcgggcg tttctgggag ctttcagaat acgagcacta aaatacgact
     5881 tgtcagaaaa tctgtcaggt gatacaatag cagacaggta actaaccaac caatacagag
     5941 catggaggaa cttatgagca gcgtaagaag agtatatgtg gaaaagaagc cggcttttgc
     6001 tgtacaggca aaggatcttc ggcatgaact ccgtaaatat ctgggagtat ccggactgac
     6061 cggagtcaga gtactgattc gttatgatgt ggaaaatata tcggatgatg tttttgaaaa
     6121 agcatgcaag accgtttttg cagaaccgcc ggtagatacc ctttacaaag aaagtttcga
     6181 gatggcagag aatgcacgcg ccttttctgt agaatttctt cctggacagt ttgaccagag
     6241 agcagattcc gcagtacagt gtgtgaaatt cctgaatgaa gaggaagaac cagtgatccg
     6301 ttctgccacc acgtatgtga tcgaaggcga agtgagcgat gaagagttcg aagctatcaa
     6361 acatcactgc atcaatccgg ttgattcccg ggaaattggc atggaaaaac cggaaacgct
     6421 ggttgtaaaa tttgaagagc ctgcagatgt ggcgatcctg gacggatttc aggatatgcc
     6481 ggaggcagaa ctgaagaaac ggtacgattc tttgaatctt gcaatgacct tcaaagactt
     6541 tttacatatc cagaattact tcaaatccga agaacaccgt gatccgtcca tgaccgagat
     6601 ccgtgttctg gatacctact ggtctgatca ctgccgtcat acaacattct caacagaact
     6661 gaagaatgtt gcatttaccg atggatatta ccgtgagccg atcgaaggca cctatcagaa
     6721 atatctggct gaccgtgcag agatcttcaa aggaagagaa gataaattcg tatgtctgat
     6781 ggatctggca ctgatggcaa tgcgtaaact caaaaaagaa ggaaaactcg atgaccagga
     6841 agaatccgat gagatcaatg cctgcagtat cgtagttccg gttgagattg acggaaagac
     6901 agaagaatgg ctggttaact ttaagaacga aacacacaat catccgaccg agatcgaacc
     6961 tttcggtggt gcggcaacct gtcttggcgg tgcaatccga gatccgcttt ccggacgtac
     7021 ctatgtatat caggcaatgc gtgtgaccgg tgcggcggat ccgaccgttt ctgttaaaga
     7081 aacactcaaa ggtaagctcc cacagaaaaa actggtacgt gaggcagcac acggctatag
     7141 ttcctacggt aaccagatcg gtcttgcaac cggatatgtc aaagaaatct atcatccgga
     7201 ttacgttgca aagagaatgg agatcggagc tgttatgggt gccgctccgc gtaaagatgt
     7261 aaagagaatg acatctgatc cgggcgatat catcattctg cttggcggac gtaccggccg
     7321 tgacggtatc ggcggtgcaa ccggttcttc caaagtccac accgaagctt ccatcgaagt
     7381 gtgcggagca gaagtacaga aaggaaatgc accgaccgaa cgtaagatcc agagaatgtt
     7441 ccgccgtccg gaagtcagca gactgatcaa aaagtgtaac gactttggtg caggcggtgt
     7501 atccgtagcg attggagaat tagcagacgg actgaacatc taccttgaca aagtgccgaa
     7561 aaaatacgca ggtcttgacg gaaccgagat cgccatctcc gaatctcagg aacgtatggc
     7621 tgttgtcgta gatccgaaag atgttgagga attcttgaaa tacgcaaacg aagaaaacct
     7681 ggaagcagta gaagtggcag ttgtcacaga atccccgcgt cttgtcctga tctggagaga
     7741 caaagaaatc gtaaatattt ccagagcatt cctggataca aatggtgcac atcaggagac
     7801 cgatgtaaaa gcagagattc cttcacagga aaataaatat ttcacaaaac cggaagtaac
     7861 agatgtaaaa gaaaaatggc tctccatgct ggctgatctg aactgctgtt cacagaaagg
     7921 tcttgtggaa atgtttgaca gttccatcgg agcaggctct gtgctgatgc cgtacggcgg
     7981 taaatatcag ctgacagaga cacaggcaat ggttgcaaaa cttccggtac tggaaggtaa
     8041 gacagacact gtaacgatga tgagttacgg ttttgatccg tatctgtcaa gctggagccc
     8101 gtatcatgga gcaatctacg ctgtgatcga gtccgtttca cgtatcgttg caggcggcgg
     8161 agatttcagc aagatccgtt tcactttcca ggagtacttc cgtcgtatga ccgaagatcc
     8221 ggcaagatgg agccagccgt tcctggcact gcttggtgca tacgatgcac agctcggatt
     8281 tggactgccg tccatcggag gaaaagacag tatgtccgga accttcaatg agatcgatgt
     8341 tccgccgaca ctggtatctt ttgctgtaga tgtggcaagt gacaagacca tgattacacc
     8401 ggaactgaaa aaagcgggaa acaaactggt tctgctgaaa atcgagcgtg atgcttatga
     8461 tctgccggat tatgagaaga tcatggatca gtatcataaa ttctttgaag atgttaaagc
     8521 aggcaggatc gtatctgcct atgcactgga cggaaatggt ctggcagcag ctgtcagcaa
     8581 gatggcattt ggtaaccaca aaggtgtcaa gattgatgca caggtagcaa aagaagacct
     8641 ctttgcagca gatttcggaa gcatcgtagc agaagtaccg gcagataaag tggcagagct
     8701 tacgatcgca ggcgttgtgg ttggtgaagt agaagacgat gcagtgcttt cctatgggga
     8761 tgtaaaagtt acaatggaag aggctctgac tgcatggaaa ggtacgctgg agaaggtatt
     8821 taaaaccgta tccggagcag agaaaaacga cggaccggca ccggagagca tagaagcaca
     8881 gcaggcagca gatggcggca cgatcgacga gaacggctgc tatcatgcag gaagcgttta
     8941 tgtatgcaaa cataaagtgg caaaacctcg cgtatttatt ccggtattcc caggtacaaa
     9001 ctgcgaatac gacagcacaa aagcatttga acgtgcaggt gcagaagtgg atgtcaaagt
     9061 attcaaaaac ctgacagcgg aagatatccg tgattctgtc aatatttttg caaaagcgat
     9121 cgatcaggca cagatcatca tgttcccggg cggattctca gcaggtgatg aaccggatgg
     9181 ttccgcaaaa ttcttcgcaa cagcattcca gaatgcaaag atgaaagaag ccgttaccag
     9241 acttctggaa gagcgggacg gactggccct tggtatctgt aacggattcc aggcactgat
     9301 caaactggga ctggttcctt acggaaagat caccggtcag acaccggatt ctccgacact
     9361 gacctacaac accatcggtc gtcatatttc caagatggtc tatacaaaag ttgtgacaaa
     9421 caaatcccca tggctggcac aggcagagct cggagcaact tactgcagtc cggcatcgca
     9481 tggcgaggga cgtttcgtgg caagcgaaga atggatcaaa aaactctatg aaaacggcca
     9541 gatcgcaaca cgttacgtgg atgcagacgg caatgttcag cttaacgacg aagaatggaa
     9601 tgtcaatggt tcctactatg caatcgaagg tatcacaagc ccggacggac gtgtgcttgg
     9661 taagatggct catgcagaga gaagaggaga ttccgttgcg atcaatatct ttggcgagca
     9721 ggatatgaag atctttgaat ccggagttgc ttactttaaa taagatttca gatgatataa
     9781 tataagcgtc aaaagtctga actgcgattg tgcctgccac aaatgagcac tttagactta
     9841 ttgaaacgcc taccaccgaa aacgtgataa aaaagttcca gaaagtgata ttgactttct
     9901 ggaactt
//
LOCUS       DAWDWH010000061         9855 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3484_length_9855_cov_15.023673, whole genome shotgun sequence.
ACCESSION   DAWDWH010000061 DAWDWH010000000
VERSION     DAWDWH010000061.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9855)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9855
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3484_length_9855_cov_15.023673"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1078..1087
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7692..7701
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8960..8969
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2387 a         2408 c         1994 g         3036 t
ORIGIN      
        1 ttgatctgtg tctggactgc ttctgcactt ggttctgttt ctgcatatac tttctccgca
       61 cttgcgatcg tatcagaaag tgctttgtaa gatgctgctg tatagtcatc tgctttcagc
      121 gtttttgcca ggtcaatggc tgccttcagt cctgcttttg tatctgtctt cggaatatcc
      181 ggctgctctg ctgctttttt cacgatcacg ataaagctta ccagcacatc acagctgtcg
      241 atgttgctgt ccactttttt cagcggactt aagttcacag tcacattgcc tgcttctgaa
      301 agtgtgatat tttccagtgt cacactgtct ttgctgccgc tgatcttgtg gtcttcgtgt
      361 acagccagtt cggttcctgc ttcatctgcc acagtaatct ttgcatggcg gtcatcgcct
      421 gcccactgtg cccatggatc atagaatcca gttaccactt catacgttcc tgcatccagt
      481 gcaaatttgt aggtcatggt cttctgattg tttcctgctt taaagttgcg gatcgtcgta
      541 taggcatccc cgcttccgtt tgcttccact tcggatgccg gatttgtgaa tccccatccg
      601 cttgtttcac tgtatgcctg atccggtact gtgtttttca gcgtatctgt gtaatccttt
      661 ctataagctt ttccttctgc tgagaaagca tttgcaccac agtccacaaa gtaaacaggt
      721 acgtcttctg ctttggttga tttttcccac tgtgcataga ggattataga atctgaaacc
      781 acagtttctt ctgttacttt ttctccacct tccggctgtg taaaccagcc cttaaagttg
      841 tgtctgtttc tggtcggtgt cggcagttct cctgcttggt cgcctttgtt cagagtacgt
      901 tttgtacttg ttccttccgg aaggcttccg ccgtttgcat ccagactgac aactgctttt
      961 ccttcaggaa ttacttcgcc accggtcagc tgcagtttgt tcatattgaa ctgccatgta
     1021 tttccggtcg gatatgcaat cttgatatag tttgtgtcat ccggcagctg tgttgctnnn
     1081 nnnnnnngtc tgccgaagta tagaaagtaa agttttgatt tgcatttcca tctgttgagc
     1141 cggttcctcg aaggacaaaa cctttgatcg ccggtgcacg cacgatcaga tattccggat
     1201 ttgcactgtt tgttcccgga tataccccgt aatcaaaacc atagttatgg caattctcga
     1261 tcagcatatt gtttgtccat ttataaataa cggacgattt ggctgtgata tcttcaatat
     1321 ctttgatatt ggtctttgtt cctgttgtct caacatagtc aaaggcttct tttacttccc
     1381 attcaccgat cttattcatc agtgccggca ctttaactac attgttaaaa tcaacgatta
     1441 cctttgcttt taccttatga ttttccggat cagcgatccc aagaactgtt ccttctacat
     1501 cgaccacatc tgcttttgat gtatcaatct cagaccatgc aatacggaat gctccggtat
     1561 gggtatcatc tacctgtact tctgctgtct gcggcaggga cggctgtgcc cctttttctg
     1621 catagtagaa atctttttct ttcagttttg cactcttgac ctccacattg gaagaaacaa
     1681 atttcgtgtc acaccagtta ccgatattct tgctgttatc tacactggaa tcttccgttt
     1741 ttgtcaccag ttttacggtc tttgaacctt tgatatccac ataaagtgcc aggttcttgg
     1801 tggcatgtgt catgacaccg gaacggtatc ttggtgtctt ttcatcatcg acataaactt
     1861 cgaaagaaac ggctccttct gcatcctgtc cgttatcaaa actcaagctg acataagcac
     1921 ggaatgcttc atatcctttt ccttctacat tataaacggc ttctgatccg ccaagcatac
     1981 ttaaacctgc tgcatattcg gtcggctttc cgccctgatc gatacgtacg ctcaaacctg
     2041 caccgtttac ggtcttgtcc ttgcttactg ttcctgttga tgtgtattcc agattacctg
     2101 ttgccaccgc atatttcaca tcgacattcg caacagcttc catatcagaa ccaaggatct
     2161 tgctctttac ttttaaggtt cctgctgctg ccacatcttc tgctttgatc tgccagtctg
     2221 tacctacaat ttctttactt tcgtcggtaa aggttacttc cacacctgcc ggcagaaccg
     2281 gaagtgttcc gattacggta gatgcatgta cttcctcgac agacttgatc tcttttacgc
     2341 ttacttctgt ttttacagta acttctggtc cgatctttcc ggtcacttca aattttcctg
     2401 cttttgcata attttcttcc ggaacttcat cccaggttac cggggcagaa ccttttgtgc
     2461 cgtctgcata ctcgatcgct gcaaactgtg gcagggacgg ttctacattt accagtgttg
     2521 ttacattgtc taccgttgcg gatttgatat ctttgactgt tacagtacat tcaaacgtgc
     2581 cttccatgcc ttctgccgtt ccggttactt tgtaagtacc tgcctggttt acattcaggt
     2641 tatccaggtt ccatccggtt acctggtgct catcgaccac accgttgctg tactgtactt
     2701 ttacggtttt tggcagaaca gaatctgcag aaatgccttt gcccatcact acggatgcat
     2761 cctgaagctt cggtgtatcc gttccgtcac ccggaagttc gtttttagaa cctacggtaa
     2821 tggtattgga tttcaggata ccattgtcac tctcagctgt cgctgtgatc acaatgtcat
     2881 cagtgcttcc ttcttctgct tttacgatca gcagtgcttt tccgctgaac gctttgcggt
     2941 tgtctgtgtt tttgtatgga tcatggtcac gcggatcacc gttgtccaca gatacgattt
     3001 cgccgccttc tacgtcgaaa gtgatacggt tgtctgctcc aggaaccatg attcctgtat
     3061 tgtcctcaat cgtagcttct acaaatacca tatcatcgcc atcgttttta atgtacgttc
     3121 tgtctgcgga aagttctacc tgtgctgcat cacctgatgt atttaccaca tcggttgcga
     3181 tcacgtttcc ttttccgtcc ggctggtcat agccgactgc tttgagttcg cctgcttcgt
     3241 aatccagtcc gctggttcct tttgtccagt agatatatac cggggaagca gtctgtttgt
     3301 cataattctt aatgccaagt gattttccgt ttaagaacag ttccacactc tttgcatttg
     3361 tgtaaatgta taccggcacc ttctgtcctg ccttgaaatt ccatttttgc ggcagaagat
     3421 gtacggtcgg tgtatccgtc cacatactct tgtacatata aacaacatct tttgcaaaac
     3481 ctgcggtgtc gatcatacca aagtaggaac tcttggatgg ccagcttctt ggggtcggct
     3541 cacctaagta gtcatgcccg gtccatacga attcaccggc tgtgtaagtt ttgtctctgt
     3601 gtgcttccag agaagcggtt gccgatgaaa atgtccagta agtcggatat tcattacatc
     3661 tgtcgccgct tccttctcct tccaggaagt aaacaccacg gtaataataa gcggatactg
     3721 tctcagatcc aaaaataacc atattcggat atctggagtg ggcaccgtca tactgtccgg
     3781 ttgcatagtt aaatccggac atttctgctt ttgccagatg cttttcctgc tgtgcattat
     3841 ttgcccagta attgtcccag gtcggcgggc atgctgcgac cggtcttgtc gtgtcgattt
     3901 ctttgatcca gccggtcagc atatccagtg tattttcacc atgtttgtaa cgtgcatcgt
     3961 agatttcgtt accggtactc catgcaaaaa tacaaggtgc gttcttgtca cggtctacca
     4021 tcgctttgat atcacgttct gcattggact gcagatcctc acgttttact tctatataag
     4081 aagtgtcatt gttggtatta tcatttccgg ctttatcaaa aacgacagtt tttccatctg
     4141 ctgctttatt gaagtagtta tgataatcgt cactgttttt tgaatacagc cactgatcaa
     4201 aggcttcttc aaataccagc attccaagac ggtcacacgc ctcgataaac tccggtgaca
     4261 ccgggttatg ggaggtacgg atcgcattga caccgtattt tttcagtgta cggattcttc
     4321 tgtcaacagc tgcctgatag gtctccatac caagagatcc caggtcagaa tgttcacaga
     4381 caccattcag ttttgtatgc acaccgttta aataaaatcc actgttcgga ttcgtagcaa
     4441 tataacggat accaaaacgg ctgtcggtac gatccacaac tttgccattc tggatgatct
     4501 ccgttcttac ccagtagagg ttcggtgaat ccacggacca aagtttcggg tttgctacat
     4561 ccacactctg gctaatcgtc tctgttttac cggctgctac ggtaatgtca tcggtctgtg
     4621 tggaaataac atcgctttcc ttgttataaa cggtcgattt tactgaaaca ccagttgctt
     4681 ctgtatcaga atcgtttgtt acgtccgtct tgatgtcaac gcctgctttt gtggcattct
     4741 tgatttcttc gtatttgcca ccttctgctt ccagtttttc aaacggagta gtgatctgca
     4801 cgccattcac cggaacattc agtttctcgg tggaaatcag atatacatta cggtagatac
     4861 cggctcctgc ataccatctg gcactgctgt tgctgctttg tactttgact gcgatcacat
     4921 tctcgctgcc atcgtatttc agatatttgc tgatatcata cgtaaaggtg ttgtagccaa
     4981 ggtactgctt ccagttgtcc tcggtatgtc cgttcaccca tacctggctg atcatctgca
     5041 caccgtcgaa ttcaattgaa acggttttgt ctttaaagtc atcggaaagt gtgaagctct
     5101 tgcggtacca gccgacaccg cctgccagag aaccctgtgc cgctctgaca ccattgctgt
     5161 tctcataagt accatagatt gcccagtcat gcggcagatc aacgttatcc cagctcgaat
     5221 catcgtagtc cacaccatcc gcctgaacgg aagtatcgtt aatgttgtac ttattgacca
     5281 gtttgaattt ccagtcatct ccaaatttca ttttacgctc actgcgttct gttgtcgctc
     5341 ctgttttctg aacggctgcc gcgacatctg ccgcttctgc tttcactgga gccggaagaa
     5401 tcccgattcc actgaaaacc atggcaacac tcaaggctaa cgccgtccat cgtctcgcac
     5461 cttttctttt tttgttcatt ttttcctcct ttttgctgct tttcacacta tccgcatcgt
     5521 gtttagtgaa tttatacact ttgcttactg gtaatataca aaatttttca ccaaaatcat
     5581 atgtactatt ttttgttttt tggtactttt tttaccgttt tccatttctt tcgctttttt
     5641 cctgtgttat actaataatg ttgaatcaaa aattttggga ggttgtatgt atgaaacgta
     5701 caaaaaaact acttctcctc tttttatttt tctgtctttt cctgactggc tgcacagctg
     5761 ccacgcagga aaacatggat tctgctgaaa acaattatac agagcttact cttttttcag
     5821 atgtctcttt ctggaatcct ccagtctgga gttttgaaga aggaagcatc agtgccggta
     5881 tctcaaagaa aaccggagca tacctggatt ttacgattcc gccacaggac gcatccaaaa
     5941 agctgagtct gatgatctta aaagatgaac tccctgacct gatcgtcgct acggacaaga
     6001 atgtgatcaa tcagctgatc cgttccggaa aggtctggtc tttgcaggat ttttttgaaa
     6061 cttactgccc ggattcccat ttgctgaaag atttcccgaa agaccggaaa caggagtata
     6121 tccgacagta cggagactgg tatgcttatc tttcacacct gaataccgat gatgcccgca
     6181 aaacctggaa agaaaagacc tcttattatg gagatctttt cacccacagt tacaatcatg
     6241 gcattatgtt taaccgcaaa cttctggcac gtgctaacct gaccgtttcg gatatacaga
     6301 ctgcctctca ggtcttaaaa gcttttgaaa aagtcaaaaa gctgactgct gaagacggac
     6361 agtccaccat cccattgctg ctggaaggca atcaatatct tgactcttcg atttcctgcc
     6421 tgatcggttc tttcggtgct gaagtcattg acgacaatgg aaattataca gaacgcttcc
     6481 ttcagccgga atgcaaagat gcttttgcct ttctgaacac agccttccgc aaaggttatg
     6541 ctttcagtga agatctgaca cttgataatc tccagatgcg cgatctgata gcagatgacc
     6601 gtgtattctg ctatatcgga aacacttcca atacaagtgt ggatgccacc aggtgggttt
     6661 ccgcaggtcc gatcctctcc gattttggaa aacgtccggt gatgagcatc gacctgagtg
     6721 taccgaccgg ctggatgcag acttttgtat ccaaaagctg caaaaccccg gaacttgtcg
     6781 cccgcttttt tgattatatg acttctgacg aaggattgct ctacagcaac tacggcgtgg
     6841 aaaacgaaga ttatactttc gactcggatg gctatatcca ccggactgcc agaggacagc
     6901 agcgctttca tgattctaat gatatgctcg gactgttctg gaatttttac aatgttgcct
     6961 gggatcacag cctacttccg gtacctgcaa aaggaagcat ggacgattgt ttaaatcaga
     7021 tccagactgc ttttgccaga tatccggaca catatgttta tgattctgcc cttcttcgtc
     7081 tgccggataa ttacatttcc ccgaacagtg aggaaggtca gattgaaagc acgctggaag
     7141 cttaccgcaa agagcagatt ccaaaaatcc tctctgcttc ttcggatacc gagtttgaag
     7201 aacagtatca gctttttgta accacacaaa aagaacttgg agctaaaaaa ctggatcaga
     7261 agatcaaccg gcaaatgcag gagaattttt cctattacgg taaaaaaata gagaaagtca
     7321 acccacaaat gcaagacacc tcaaaatcaa acggagaaac aaaaccatga acttcttaca
     7381 gacttaccgc agacacctga tctttaagct ggccgggatt gctgccttga tccttgctgt
     7441 gctttcgtct gcctatcttt ttatgtcggt ggactatgca agatctgtgc gtaaaaacac
     7501 gctcgccctg aatgaacgtc tgacagccca gactgccaca cgtatcgaag cgtactggga
     7561 ctcgctttac aatatcacaa ctgcattctg ttattctcca accgtgcagc agtatttttc
     7621 ggtaaattct ctgaaccgac ttccggatac acaggagctt gcctcggtct tttccaacac
     7681 aatcctgctg annnnnnnnn nacaatgcag atgcaaagca gattgcttcc atggggaaaa
     7741 atttttcgat tgatacttcc aaactgaaca ccgatgccgt gatgaatatc cgggcatcac
     7801 atctgaagtc aaacgaaaaa cgcccttact atgagctgat cttccctgtt tatgatctga
     7861 acagcatcca gtaccagaag ctgcttggta cctgcgtttt tgtcctggaa ccggaaagtt
     7921 ttgatgatac acttcaaaat gcccagtcaa cggagcatgc cgttgttttt cttctggatt
     7981 cctcggactg cattcttgct tctgccggtt catcgcagtc ctgcggaagt acgctggcat
     8041 ctgagatcct gcacagcact tccgaaaaat attttttcac tcagacactt tcctacaatg
     8101 actggaaaat tgccggtttc cttccggaat cagatcttcg gaaaccggat cagagcctga
     8161 aagaaacaca aatcctggtt tattgcattt ccctgtgtct tttgctcttt ctgcttctgt
     8221 actgcaatta cagcatcata aaaccgatca ccggaatttc tgcttttatc cgtgagatca
     8281 atcagaatcc ggactcccgg ctttccctcg accgtccgga tgagatcggc acagtcgcag
     8341 aaagcctgaa tcagatgctc gatgaaaaac agcagttgca gctggagatc gagcaggtaa
     8401 aatatcttgc ctatgaaacg gaactttcca aaaaacaggc agagatcctt gcctatcgaa
     8461 gccagatcaa tccgcacttt ctctataata cgtttgagtg tatccgggat atggctctgt
     8521 tctacgatgt ggatgacatc gcagaactta cgatggctct ctccaatgtc tttcgttttg
     8581 ccgtcaaggg aactgatatg gttaccgtag aaaatgagct ggatcatatc cgtgaatatg
     8641 caaagatcat aaattaccgt tttatgggca aaatccgcat tgaaatcgat gcagaaaatg
     8701 ctatactgaa ctgcagcgta ctgaagcttc tactccagcc gctcgtggaa aatgccgtct
     8761 ttcacgggct tgaacaaaag attgaaaacg gtaccgtgtg ggtacgtgtc ttcagttccg
     8821 atgaccagaa actctgcttt gtggttgaag atgacggatg cggcatcgaa ccggagcatt
     8881 taaaacagat caccgcctct ttggaaaccg aaaaaaatac aaccaaaatc ggcgtcttta
     8941 atatttatca acgactgaan nnnnnnnnna ctgaaacttc attatggtaa gcagttttct
     9001 tttgacataa aaagtatctc cggcaaagga acctgcatta ccattatgat tccacaaaaa
     9061 atgatgacag aacctgaagg gagtatttta tgataagtgt ctatattgca gatgatgaag
     9121 cctggatcac cattggtcta aaaaaactga tccagaaatc cgctcttcct tttaaagtga
     9181 tcggagaagc acataacggc gtaactacgc ttgaagatat caaagctttg aaaccggatg
     9241 tcctgtttac cgatatccgg atgccgggac tttctggtct ggaagtcatg tcctatcttt
     9301 cagaacatca gattcccacc aaagtcgtgc tgatctccgg ctatgcacag ttcgaatatg
     9361 cacgccaggc aatgctgtgc ggtgcttttg attatctgct aaagccaatc cggcaggaaa
     9421 cactgaacaa aaccctgtca cgcctgcaaa gtgaacttga gcctgccgct tttacaaccc
     9481 ccgcagctgc agctgaaaac acaatgatca ctgacttttc tgccgccgga agttctctgt
     9541 ttaagaaaat ccttgcctat atccaggaac atgataccga agagctctct ttaaccgccc
     9601 tggcagaaat gtacaatatc agctccagcc gcttaagcac cctgatcaaa aaggaactgg
     9661 gactgtcttt ttcggaatat atcacggcaa gacggatgca gaaagccaaa gagctgttaa
     9721 aagatgacag ccgctccatt gaggagattg caaatgcatt cggctaccac gattaatttt
     9781 atgttccaaa agttttcaaa aaaacgcatg gaatgtctcc acgcaaatgc cggaagtatc
     9841 tttaaatttt tcttg
//
LOCUS       DAWDWH010000062         9826 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3490_length_9826_cov_14.980759, whole genome shotgun sequence.
ACCESSION   DAWDWH010000062 DAWDWH010000000
VERSION     DAWDWH010000062.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9826)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9826
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3490_length_9826_cov_14.980759"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8653..8662
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9228..9237
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2492 a         2771 c         1688 g         2855 t
ORIGIN      
        1 cctcatgcga aatccctgaa aaagtgccac gattgccaga atgatcacca aaacgcccgc
       61 ttttgcaaga tatgtaagaa attgaccgat atccagacgt gcctgttctt ctgacacttc
      121 atattcttgc agaacctcct gtaccagctc ttccctcgtc ctgttttttt ccatatgttt
      181 ccacagaaac gccccgctct cattcaaggt caccagaccg ttaaaagtaa gagccgtatt
      241 tcccaccggc accaggatgt tttcccctgc tactttccgc aatacaaatc tcttcgcctc
      301 catatttcac tcactttccc ttgttgtatt catcttctct tttccccgaa gatcccatat
      361 tctcttaaaa tctctttcct ttttcttcca gtcttaacga tttcttttac cgatcctaaa
      421 accggacctt ctctgagata gccacagagc ctctgccccc aggcaaacgg cagaagatat
      481 ggatgctttt tcaggtacgg aaaacgccac agaagattct ttgcaggcgg aaatgccatg
      541 taaaacagac cggggaatat gcctgacagt cctctttttt ccgccgcatg cagcgtaagg
      601 gtgccgctgt gcaggcgact ccttgtccct tttccatgaa tgccggagtg caggatatct
      661 tccatcagtc tttccgtatc cagtttcata ctatgcacac cttccggaaa atagaactgt
      721 ttttcttcaa aaccaagata ctgcacacca aggcgaaaaa tcgcctctgc aaacaggatc
      781 gcatccgtca tccggatgcg atcccagaaa atctcccagt cgatctcctt ctcgtaatac
      841 tcggcaaaca gcagaatatc gcagatctgc cgtatcccga ctccggaagc aagcagatgt
      901 tttctggcat ggcagaactg aaaaaacaga tgatccgtcg gctgcggcat attgattttt
      961 acaccttcta cctctctttc gatcgcctgt ttatggatct catcaaaaaa tccattccat
     1021 ccaatacacc atttttcatt ttccggaaac agcatccggt gcagctcgat caccagaaag
     1081 ccgcctgttt ttttataagc cgtctcatag ttctcggtgc cagtctcagg atttaactgc
     1141 tgcattccat ataaaagcat cgcttcctga caggcctcaa actgctcttt tggtatcagc
     1201 agatcctcat cagcagaaag cctcgcatcc ggattcggat acagactgcg gcagacgatt
     1261 ccttttaccg ccagcggctt tacacccatc tgttgtaaat gagcgtacaa ctccagaaat
     1321 tcttcggtca tcgtcctctg aagtgccatc agccgtagac tcttttcccg cacttttgca
     1381 aacatcacac ggtccgccaa agccgctgcc cggcactccg ccaaagcctc ataaaccagc
     1441 ggcagcacat gatgcatatc cgccagggca aaaacccctg accactcctg cacggaacgc
     1501 tccttccagg ccacccgctg cccgcggatc cccgcacgca gtgcctccag aaaaagctcc
     1561 tggtattccc ttgtttttat gaacatttca gtcctccctg caattcagaa aacatttctg
     1621 atattttcat tgcaaaatcc tgccgaggca tcttttgacc tcaacatcat atgatgttgg
     1681 agtcaaaaaa agaccacata ccgttttggt atatggtctt tttatcttag tgggagaaga
     1741 ctccctcttc ttctattcgt gctcttcctt aaccagatat gcctgtacca ggtagcggta
     1801 attcttaccg gccgcatggc aggttgagag cgtcaagatc ttgctgtcct tgtccacctt
     1861 cgccttcgtg tcaatggatg cattcatatt tcgctgctct aaaatatctt tcagataagt
     1921 ctccctgctt tcctcatctt ttccctgatt ataatacatc agcacatgac ggttgtccgt
     1981 cagataggcc gcaaaaatcc gatatttcaa gattgaatcc ggcgtgtaga tggtcagatc
     2041 cttatgatct ttcaggaatg tcttatcctc gtacttgtgc agacctgcaa acatcgaacc
     2101 gtctttcatg ttatgtccgt aaagcaccgt cagataatca tcaaaatctt tggcgttttc
     2161 cttttccgaa taaatcgctc cgctgtcact taaactggta tccgccgcat ggttcagata
     2221 gtacgtatta tccgtgctgt cctgtacaac cggatagtca atatccgttc ccggtatcgt
     2281 gatccacgca taaatgtccg gattttcctt ctgtaaggtc tcaaaatcca ccggaatgtc
     2341 cagcttcttc tttttttcct ttgtcttgga ctgttccttc gactcctgcg tatcggtctg
     2401 ccgtttctcg gaattctgct gctgcatttc ttcataagtc tgcctggcat gttctctttg
     2461 tgtataaatc cgcaaaacga ctgccgtcag gatcactgcc gcagcaaacg atatcaccgc
     2521 gatcaccttc catgttcttt ttttcacttc gactcacctt ctgactgttc ttcggtatag
     2581 aaggtctgaa tgatcagatc ataaagtgca tcatcatcca cataaacctc atcatagaca
     2641 ctgcccttct tggatttcgc cttcaaagaa acaatgccgt cggcagtaaa atgcatatcc
     2701 atcgcctccg ttgccagata aaccgaacga tccaggctga gatttgtgac catatcatcc
     2761 gtaagactct gataaaggct gactggaaga ctcatatcct ctttcatcgc cttcaccgcc
     2821 tgtcccataa aattcacaaa gtactgtttc tgacgctcca tacgagaatt gttggaaaaa
     2881 tccttttccg tatctcgata acgtgtaaac ttcaacgcct gatctcctgt cagcgtcacc
     2941 gccgccccct ctgcaaagga cggatcaacc tgcgtcatat cttccggtac cgtcaccgtt
     3001 acgccgccaa ctgcatcgtt gatcatggca accgcattca tattcaatgc cacatagcca
     3061 ttgatcggca ttccatagaa caggttggag acagcatcca ccatgtactg acagcttgtc
     3121 acctttccat ccccgaagga atacgcaagt ccgagatgat tctttgattt tccaagataa
     3181 tttcccttat aatcaaacgt cttgatctgt gtcatggtat ctcgcgaaat actgatcatt
     3241 ttgatctcat ctttgctctt atccataacc atcagaaaaa tgctatccgc ctgaccgctc
     3301 tcaccggaaa catcatcctt ttcctcgatc gtatcactct cctgatcaat gcccatgacc
     3361 agcatcgtca ccagatcgtg attatagcgg taagccttgc cattgtacca gatcacatcc
     3421 gggtcggaag aaacctttgc atcacttccg tcagtgtttt ttgaaatcat ctgattttct
     3481 gccacactga tgttctcatc catatgttcc tgtccggtcg tacgcagtac atgataagct
     3541 ccgaacgccg ctgtttccag aagcatcaac acgatcagaa tactcagcat cagacgcatg
     3601 gcataagaga acgcactttt cctggaaaac gcatatttcc aaacgatcac aaacacaata
     3661 ccaataccgg caccgatcgt atgaaagagc agattcccca gatcaaaaac tcccgtcttt
     3721 aagacaagct ggagtatttc cacacaaagt gctaatgcaa acgatgcacc catactcttc
     3781 gcagcctgat actttccttt catcaggatc atgatcaaag ctccaagtgg aacaaaagcg
     3841 accgtacctg ctgccacttt taccagcgtt tccatcaccc agcttcctgt gaagatcccc
     3901 atatccaccg cagaatctcc ggatcttttt ccaagaatgg tcaccgccag gatcagcagc
     3961 acataaatcg caaacatcag ccacacacag atgatcctgc cacgctttct ctttttttct
     4021 ctccaggatg gtgtcacaaa aacgtacaca ataagcagaa gcagaaagct tgcggctgcc
     4081 gccgcatata ccatgtccgg cagtacgcca agtggagtcg aaactgcctc caggcctctg
     4141 ctgatactcg ttaatactcc cataatccta tcctttcact accagttcca gcgtaaccgg
     4201 agcagataca ttgccgtcac tgtccatcgc cgtgtaagtc agcgtataag tgcccggttc
     4261 atccgtgctg tagtcaccat ggacctcaat ctgacggaac agagcagaac gatcatcggt
     4321 atcatcagtg atctcagata cctgcgaaac cgcatcaaac gcatcgccga ccttgatctc
     4381 tacctttttc tgctttaatt tcaggaccgg ctttgacgga tctgcagcgg ctgaagtctg
     4441 ggtttctgtc tcttttgttt ccgtttcttt ttccgtttcc gatgccatct ctgtatcaga
     4501 agtttctgtc tttgcagtac ttgcttctgt ttctgtactt tcgcctgcct gcgtttcttt
     4561 ttcacttact ttcgtgcttt ttttcttatt cttttctgtt tctttcgctg tttctgtctg
     4621 aagctcatac ccggcaacca gcatcgtctg ctgatcctca ccctgcatct ccagtttctt
     4681 cccgatttct ttataagagg tcgcagccat catcacagcc actgctgctg tcacaatcat
     4741 tgcaacggtc agccatcttt ttcttctcat ctgttatgcc tccactataa ggaatcttcc
     4801 gtgtccatat cgtcttcttt cacatctaca tatctctgat actttccata tttgccgtag
     4861 ccatactttc catatccgta ttttccatat ccatattttc cgtaagaagc gaatcccttc
     4921 tgacttctgt tgatcagata gccaagcagt ttatcctcct gaataaaaga ttccattcct
     4981 ttctcaatct gcggaatttc tgccatgtca tgacgtacaa cataaagtac acgatctgca
     5041 tatcccgcca ggatcgcagc atccgaaaac agatatgccg gcggtgtatc gatgattaca
     5101 tagtcaaatt ctgacttaag aaaagtcata agttcttcca ttctgctctc atccatcagg
     5161 ctggatatat tgccatgcac ctttccaccc tgaattaccg tcagatctcc ttttttctgc
     5221 aaaacctctt taaccttggc atttcccttc agatactctg caagtccctt ctgttcttcc
     5281 attccaagca tctctgcaac ggatggcttt ctgaggtctc catcgatcag aactgtcttt
     5341 ttctcatgct gcataaatgc cagtgccaga ttgactgccg tcatactctt tccttcctgt
     5401 ggaagcgtac tggaaacgag cagaacctgt ttatgctcct gttccatctg tttctcgata
     5461 cgaagctgag ccgcctgcac agactgctta aatccccagt ccacacgctt gttggtcagc
     5521 agaagctgct gttttttact cttttcacgt ttcttgactg caatctccgg tatcagactg
     5581 atgcatcctt tggacgtgat cttcttcata tcactttctt tgcggaccgt cctgatcagc
     5641 tgtgcataca atgctgcgat tgccagacac aagatcacgc cagccaacac accctccacc
     5701 agctgcttca ttgctgaata accatttacg ggattttcgc ctgccaaagc ccgatccaca
     5761 acctgtaatt caatcgtacc attggatcct gccacccatt ccggatacat attctccaga
     5821 agatccagca gcatattggc attgttataa ttgtttgccg tcaccgtaac agaaaataag
     5881 ttggagttgt ccgtatatgc agatgatatc acataattcg gattgacatt ttcttcttcc
     5941 acatctgcaa acaggcgatc cttaaaatct cccgttttcg taaccacgct gatactgttg
     6001 ctcagtctct gtgcaatgga agcatctgta tccgaatctc cggtcttatt taccgcataa
     6061 gtcacttttg cctgatattg tggaacatac tgtctgtttc cataaataat aaaaactgca
     6121 gtcacgatca caatcagcaa taaaaattgc agccagtact tctgaaaaca ccgcagtgca
     6181 tcatccatca tgctgaaaag atcaattcct tcctgaccgg caccatattg ctgtcctgtt
     6241 ctcactcttt ctgcacgtac cttatcactt tcctgcatgc tgtccatcct ttccgtaagc
     6301 gtatccgtag taatagcttc catacttata cttgtgatag ccgtttcctg caattttctc
     6361 gccctttgcc atattcagga caccaccgac tacgacagat cctgcatcct ccagaatatc
     6421 gatcacatca ttgaccgatg ctgcatgcac ctcatcctgc ttcatgacca taaaaacaga
     6481 gtccgcatat ccggcaatga tctccgcatc acgtacaatg ccgattggtg ccgtatccag
     6541 gatcacatag tcaaaacttc cggacagttc cttcagcata tgttcaaatc gctccgaatc
     6601 aaacaatttt tccggctcag agactgcttt tttctgcaaa atacaggaaa acttacgctc
     6661 gtcctgacca tattcaatca aaacatcctg aagtgtactc tcttctgtca gataatcata
     6721 gagctgtttc tgatctttca tatcaatatc aaaaagctta cagacggcag atcttctcat
     6781 atcacagtcc accagaacta ctttcttacc acgctgcacc agattcagta caaggttcgc
     6841 cgctgccgta gattttccgt cattctcttt cagactgctc accatcagca ctttataatg
     6901 attttcgtgc atctttctgc gaagctttgt aacgatcttg tccacagctt ccgcatactg
     6961 cgaagaagtc cttggatctg tgatcagaat cgacttctgt tcctgttttt tctttacaaa
     7021 cggtaacgct ccaaagacat ccatatccag caactgctgt gcctgttcga cactatgtac
     7081 cttgtcagtg aaaactgcca ttgctccgac cagaccacat cctgcaatca gcaccagaag
     7141 tgccaccttc agtgcacgga ttggtgcatc tgcatgattg accgtaatcc cattcccttc
     7201 aaaacttcca atcttcttca gcagatatcc cgtctggaaa tatccggaaa gtttcggata
     7261 attatcaata ccagctttca acaggcggca tgcactctcc gccgaactgc ttgtcgcact
     7321 catgataatg atgttggtac ttccggctgc atatgccgaa agatttccct tcagcttatc
     7381 cgcatccttt tcctttttta tctgctcttt catcatatca ctgttcaggg cactgacgca
     7441 tcgagatacc gccgaactga tcccatttga attgaatttt gtgcctgcat tgtctcttgg
     7501 gatcacatag agattgacac ttgccgtgta agtatccttc ttatagtgat ccagaaaaat
     7561 ataagaaaac acgcctgcca gacatgcaaa catcagaata aagagtgcat ttctcatcag
     7621 gcagcgactt atgtatttca catcatacgg ctgtgtattt gcattttgat aatgttcctg
     7681 attcatctgt cccctcctac tcgactacca caatcagatt tgcattgcag tcgtatcctg
     7741 tttcctccga tgtataatga taatttaccg tataaactcc agccttttcc gtatcaacct
     7801 tcgaagtgat ttcaatattc tccatggaaa tctgcttact tgccgtactc tgctgttcgc
     7861 tctctgaagt cccctccaca gatgaagtta ttcctgaaac tgcaaaggtt tctcctccct
     7921 cttcgatatg atccagataa gcacttgcat ccagctgttc gcccttttcc atgtatacaa
     7981 tatactgatt caatatgatc tgcggacgct gatttcgctc atcgtatcca tcctcataga
     8041 tttccacact gatcggcagc gtcgtcgtat aaccgacact gttcgtcaca ctgaccgtac
     8101 attcataaat ccccttcttc ggctctcctt ccagaacttc cttatcacca tccaaagtaa
     8161 taaatggtgt gacatccccg tcaatacagt cgtccgccgt aaaataagac aacagatcaa
     8221 tctcctgatt ttctggaaaa cgcaatgcct gttcaaagga aaaatgtgcc tgacgatagt
     8281 cagaataatg aagtgtcctt gtaagtgatg ccatattgct tgatttatca aaggccacat
     8341 aagtaatcag gaaatcactg tccgtgcctt ccttcttttt gatactgtcg atcagtatgc
     8401 tgtccgatac gtccccgtcc ttctgatcaa atgccgtcac gccggagatc agcttcttct
     8461 catccgcttt aatgcttgcc gaaaacttct ccgcatccgc actgagaacc ggcggttcca
     8521 tatccttttg cttctgtgca tttgaataat aaacacagcc tccaaccagg gaaagaacac
     8581 aaactgcccc tgccaagacc tttcttttgt ccataatcgc tgtactcctt tatgtagaga
     8641 gaatcggcat ccnnnnnnnn nntctgttca tcgatggggt acgatactca aagtcatgtg
     8701 catccgaagc cagcacactg acaatcccat catccagaaa ctgttttgca agatttttgc
     8761 atcttcttcc aaactgccca agaacactcc ctttatttac ctgaagtgca cagccctgtt
     8821 ccgccagaaa gtaggcaaac tcaggatccc tctgcacaaa aagataacgt tccgggtgtg
     8881 ccaaaactat attatacctt ctcgtctgca aatcggcaac atatttgtac attttttcga
     8941 cattttcttc aaaatcaaac tccaccaaga catatcttgt cccatttaac gtcagaagtt
     9001 cccccagatc cagcagatct gcggccttct catccataaa aatctccata cctgaataaa
     9061 gcttcaccgg aatctctctg tcacgcagtt ccgcctgaag cctctcaaaa ttttccctgt
     9121 attcctctaa cgtataatca taataattcc cgtgactggt cgcagccata tgaaacactc
     9181 cgctgcgtgc agcaagatcc gccatccgca ccgaagtctc caaatcannn nnnnnnncaa
     9241 cagcttttca caagcaatgc tttctttttt gaaaagctat accgcctatc caggctccca
     9301 gcgtattgca gatcacatcc gtcacctgtg ccatgcctgt cccggccgca tactgccagc
     9361 tctcgatcac aatcgccaga accagtgcca gccagatccg atgccagcct atcagctccc
     9421 caagcgtaag tcccagtgga aagtataaaa agacattcat cagtaactcc cgaaagaact
     9481 catccgaata agatgccatc accacaaact gatgcacact ggaaggtgtt ctccccagca
     9541 ccgtgaactt gaggatcaga aaaaacgata cccctgctcc aaacaaattt aaaatcttcc
     9601 agatgtcgat tcttatatac cttgctaaaa tccgccagag gatgagcaaa aacaacatga
     9661 tcagaaagat tcggtgataa ttccatgcat aaacatttac catttatcca ataacctcat
     9721 caataatctt ttccttttat ttttctcttt gaaattccga tatcttgata atacccatta
     9781 caattctctg ccagataatc tgccagataa agtcgttcct taaact
//
LOCUS       DAWDWH010000063         9809 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3497_length_9809_cov_18.653373, whole genome shotgun sequence.
ACCESSION   DAWDWH010000063 DAWDWH010000000
VERSION     DAWDWH010000063.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9809)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9809
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3497_length_9809_cov_18.653373"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3077..3086
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2498 a         2505 c         2003 g         2793 t
ORIGIN      
        1 ccgggcggat tcgggacttt aacccgttag aaacgtgcgc cgctaggcgc acttaacgta
       61 aaaaggacaa cagattttct ctgctgtcct ttgtatattc tgcatgatta catgatgtac
      121 gtctctgcac ttttctgttt ttagtatgga tagaagtttc cgtttgcatc ggttacgcca
      181 tcgttaccga atgtatactg cattccgccg ctgtctaccc agtttccgtt tgcatcctgt
      241 gtcacggaaa tataattgcc ctggtcatca taaaaaccgt tggtgctgcc gccggtatta
      301 cctcctgtgt ttccaccggt attgccattg tttccattgt tgccattatt tccgttgttg
      361 ccattatttc cgttgttgcc attattattt ccgctgttgt catatggata ataattgccg
      421 taattatcgg ttacaccata ctgaccaaag gtgtaaacaa tgccgtttcc atctacccag
      481 tttccgttgc tgtttttata aactgtgatc agatttccct gattgtcata gaaactgctg
      541 gtctgagtag ctccgccgtt gttattgttg ttatcaccgc cgttattgtt atttccgccg
      601 ttgttgttat tattgtcgct gtttccaccc tgcggattgt aggtaaagct tgcaccgttg
      661 ctgtttgtga taccgtttgc attaaaataa taaatcgctc cgctgctgtc tacccagttt
      721 ccgtcgctgt cctgatagat ataaactgcg ttgccgttgc tgtcataaac atactgaccg
      781 ttctggttgg aatcagaatt gccatcggta gaagctgtgc ttccggactc ggactgtgtt
      841 gtactgttcg actcggaaga tgttgtatta ttctttttag tctcagatgt tgttgtgctc
      901 gatgctttca gcagtttcag actctccaca gaagtacgga tcgcttccat tgccgttgca
      961 tcatcctgca taacctgacc attgaccgaa taaatatcct catctcctac caggttatac
     1021 gctaccgagt acgcatactt actgtttgta ctcttacatt tgatggttgt gtgatatgcc
     1081 tcgtaagaac ccatggtatt tttcttgtat tctacaactg cataatcggt tttggatttt
     1141 ccggctttct ccagattatt cagtacttct tcttcagagg acggaaaaac aagtccgttc
     1201 gtctcatctc cggaataatg tacgatcgtg atcgttccct ggttcggtga ctcaaaggtc
     1261 cattcttttg tgccttcttt tttcattgtc caggtgtcat ccggaagttt gatagaaata
     1321 ctgccatctt ttgatttaaa tgttgtggat gtatttgtag ctttctttgc tgtttcgctc
     1381 tctgtctgtt tctctgtaac acgaatcgtt tcgctgctcg tctcagtttc ctgtgctttc
     1441 tttttacagc cggtagccag acaggaaatg gacagagcga gtaccagaca agttacgatt
     1501 tttgatctca ttttcatcta tactcctcca ttctggttgt taccttcttt gacttccata
     1561 ttctacgctt tcgcagatat aatgtcaatc aaaaaccggt ttaatatgga tagaaaacgc
     1621 cattctgatc ggttacgcca tcatcgccaa actggtaggt cataccgctt tcatcaaccc
     1681 actgtccctg gctgtttttg tgaacggtga tccaggttcc gccatcatca tagaagccat
     1741 tgctatcgcc ttcaccatta tcggctgccg gattatcgat gttattctcg gtatccggtg
     1801 cctgtgtttc cggctgctgc actacctctg tttccactct tgtctccaga tccggatgtg
     1861 tttccggtgt ctgcgtatcc tggctctgat tctggtcatc cgatgcctga ttctgatcct
     1921 ctgcggctga agtctgggca gaatcctgac ctgctccccg ctgtgtctga tcctgggtct
     1981 gttttgtgct gctgttcgta tccccactgt ggctgccgca tcctgtcatc agaagagccg
     2041 atgagaggac tgctgctgcc ataaaatgta atgccttatt tttcataata atatttacac
     2101 tcctttatat cctgttttac catttgctga aattgccgtc gctgtctgtt gccccttttt
     2161 catcgaaagt gtaggttttt ccatcactgc cgatccagtt tccgtttgca tcctgtgtcg
     2221 ctgtctgata gtttccattg atatcataga aaccaatgac catgccatta tctgaagaat
     2281 tgccggaacc tgtcaaagaa tccgactctg atccggaacc tgttgttccc tgaggcggat
     2341 cataataata ctgtgtgccg ttggaatctt ctacgccgtt ttccaggaaa atataagaag
     2401 ttccgttatc atcacgccag ctgccgtcct cagacggata agcgtagatc gtatttccgt
     2461 ttgcatcaaa gaaatagcgg tattcctcat cggtcgatgc accggaagaa tcgcctgtac
     2521 tttcaccgga agtattgtct gaagcagtat cacctgtatt ttccggatca gaactgttac
     2581 cggcattatc cgaagatgta ctgccgtctg ccgaagatcc ggagtttgca ccagaatctg
     2641 tctttttact gtcatcattt aaaactttga agctggtaac ggcacttttc actgcctgct
     2701 gcaccgtact gtcactggat gtcagttttg ccgtgatcgt ataactctcg gtgtccgtat
     2761 tgatcagata cttgatcaga taaacgtaac caagatctgc gtcctgcgtg gatgcagcat
     2821 aataataaag ctctgtacca ttggtgctgt cttttttata ttctacctga gaaaatgcat
     2881 tctcctggat atcgtttaaa agagcaatcg catcttcctg agactgcggc agattcttct
     2941 ccttgatctt tgactttttg tgcgtgatcc ggatcgtgcc gacgccttct ttcataaatg
     3001 cccatttatt ctcaatgcct tcctttgtcg ccacccatgc agaatccgga agatcaaaag
     3061 aaaatgaatt gctctcnnnn nnnnnngtat atgttgtggc tgcttcggtc tgtgcagtac
     3121 tttcgctctg agctgtcgtc tgtttcgtcg tctccgtctc agatgccttt ttctggcagc
     3181 cgttcatcat aaacattgcg gccattccga ttaccagtaa tgtcgcaatt ttctttttat
     3241 tatacataaa aaatccccct tttctctctg attttcagtg tgtttcaagt ccactccctt
     3301 ttttcattgt acaatgaaga tgcggttaat gtcaactttt cttaacactt cttaacaaaa
     3361 tctacatatt tggcacaccc tgtgcccttc tctgtttcag ctttcggatc acatgacagt
     3421 acgccggaat caggaaacag tctgccatga tccatgccag aggactggca aaacatacgc
     3481 ttgcgaatcc cagatacgga accataatca cgccgataat ggctcttgcg atcatttcca
     3541 tgacacctgc aaggattgcc agaccgctga atcctgctcc ctgaatggta aagcgaaccg
     3601 cattgacaaa aacaagtgca atgtaaaatc cgctgtttgc aagaataaac agcactgcat
     3661 tatgtatgat ctccgtttcg gatgcattta tgaacagaag catccagtat tttgcagaaa
     3721 aaagcatgac caggaaaatc aggatcgagt atgtgatacc gatcagaacg gatgccttta
     3781 atccctgacc gatacgctcg atcttgcctg ccccgacatt ctgtccggtg taagttgcca
     3841 tggccgaacc gatcgcatca aacggacacg ccaggaacat cgaaagcttc gatgccgccg
     3901 aaaccgaggc aacggctgcc gaaccaagtg tattgatacc ggtctgaagc acgacactgc
     3961 cgatcgctgt gatcgaatac tgaagtccca tcggaatacc cattttacag aggataccga
     4021 tgtgtttgcc atgcaatttc cattctcccg gctccatttt caggatcggg aactttgtct
     4081 ttatacacca cagagacagt accacggaaa atgcctgtgc aagcagtgtc gcaaaagccg
     4141 gtccacgaac gccgagtttc aaaaccgtta ctgacaggat atcaaatccg atatttgcca
     4201 ctgctgccat cagcagaaag atcagcggcg atttggaatc cccaagagaa cggatgatcc
     4261 cggtcaggag attgtaggca agtgtgatcg gtatccctgc aaaaatgata aaaatataat
     4321 catatgcaag atccaggata tctgccggtg tcttcatggc gatcagcacg ctgcggcata
     4381 aaatacagac tgtgatgctc atgatcgccg caaaaaaaac agtcagccag atgctgtttg
     4441 ccacaaacct tctcatatct ttataatctt ttgccccgta acgctgtgcg accggaatga
     4501 caaaaccatt ggcaagtccc atacagaatc cgatgatcat aaaattgatc gccccggtag
     4561 aaccaacgcc cgccagtgca tcttttccaa ggaactggcc tacaacgatc gtatctacca
     4621 tgttataaaa ctgctgcagt aaaaacgtaa aaaacaatgg aatcgaaaat cgcatgataa
     4681 tcttccaagg tgtaccttct gtcagatctg ttgccgcgtt tctcatttcc ttaattacct
     4741 ccatatttta aattccattt tttgcacaga tatccgccag gcagcatctg tcacagtaag
     4801 gctttgttct cgctgtacag acctctctgc catgataaac gagtctgtgg cagaaatcac
     4861 taccttcttc cggtggaatg atcttccaga gtgccatttc gacttttttc ggatctttga
     4921 tcccatctac cagaccgata cggtttgtca gacgaatgca gtgcgtatcc gtgacgatcg
     4981 caggcttttt gaaaacatcg cccatgatca ggtttgcact ctttcttccc acgcccggaa
     5041 gctttaaaat cgcatcaaaa tcacacggca ctttgccgtc gtactcatca cgcagcattt
     5101 tcatgcatgc actgatatca cgtgccttgc tttttccaag accgcatggc cttacgatcg
     5161 cctcgatctc ttccggtgtc gcttccgcaa gagcatctac atccggatat ttcgcataga
     5221 gatcctgaac cacaacattt accctggcat ccgtacactg tgccgcaagc cggacactga
     5281 ccagcagttt ccatgccgca tcataatcaa gcgtacagtc tgccagagga tattccttct
     5341 ttaaacgctc aatcacttcc agcgcaagct gtttttttgt catatgctcc tccttatata
     5401 tttccaaaaa tctttttgta cccgttattt tatcacaaga atcgcagtaa ggatattcct
     5461 tttttttcga ttgtgcaacc cctttctttt atggtaagat aataagatgc caggatcaaa
     5521 agatctaaat ccacatgagc ttgctcataa gtggatgaaa gaccttgaaa cccgccccga
     5581 ccgaagcgga gcgtagaaag aattgtgaaa gtacgaagta cgaaacaatt catatggcat
     5641 ctcataactt tcataacttc acaaaatttg acaggagtaa ttatgatctt aaatgtaaaa
     5701 aatctgacgc atggttttgg tgaccgtgcg attttcgaca atgtttcttt tcgtctcctc
     5761 aaaggcgaac atatcggcct gatcggtgca aacggagaag gcaagtccac gtttatgaat
     5821 atcatcaccg gaaaactgac accggatgag ggaacggtcg aatgggcaaa aaatgtccgt
     5881 gtcggctatc tggatcagca ttctgtcctg gaaaagggca tgacgatcgg tgatgtcctg
     5941 cgttctgctt ttcagttttt atttgcaatg gaagcggaaa tgaaccgtat gtatgaacag
     6001 atgggcgatg ccacaccgga agaaatggaa cgtctgctcg ccgagaccgg cacatatcag
     6061 gagcttttag agcagcacga tttctatatg atcgacacaa aagtagacga gatcgcccgt
     6121 gcactcggtc tggaagagct tgggcttgac cgtgatgtaa ccgatctctc cggcggacag
     6181 cgtacccgtg tactgatggc aaagcttctg ctggaaaaac cagacatcct gcttctggac
     6241 gagccgacca actatctgga tgagcagcac attgaatggc tgaaacgcta tctccaggat
     6301 tatgaaaatg cctttattct gatctcccat gatattccgt ttctgaacag cgtcatcaac
     6361 ctgatctatc atatggaaaa ctgcagactt gaccgctatc cgggcgatta cgataaattc
     6421 caggaagtct acgccatgaa gaaagcccag cttgaggctg cctacaaccg ccagcagcag
     6481 gagatcagcg accttaagga tttcgttgcc cgcaacaaag cccgcgtcgc tacaaggaat
     6541 atggcgatgg cacgccagaa aaaactggac aaaatggaca tcatcgaact ggatcgtgaa
     6601 aaaccaaagc cggagtttca ctttaaaacg gcccgtactt ccggaaagat gatcttcgag
     6661 acaaacgatc tggtgatcgg ttacgacgaa ccgctgacaa aaccgttaaa tatccgcatg
     6721 gaacgcggcg aacgcatcgc cctgaccggt gcaaacggta tcggtaagac cacgcttcta
     6781 aaaagcattc tgcagctgat caaaccgtat tccggcgaag tgcatcttgg cgattatcag
     6841 catatcggtt attttgagca ggagtccgat acgcagaaca cttcgacctg tatcgaagag
     6901 atctggagcg aatttccggc aatgacacag tacgagatcc gttccatgct ggcaaaatgc
     6961 ggtctgacca ccaaacacat cgagagtcag gtgcgtgtac tgagcggcgg cgagcaggca
     7021 aaagtacgtc tctgcaaaat tttgaaccgt gagagcaata ttctgctgct ggacgagccg
     7081 accaaccacc tcgatgttga cgcaaaagca gaactgaaaa aagctcttca ggaatataag
     7141 ggaagtattc tgctgatctg ccatgagccg gaattctatc aggatatcgt gacaaccgtc
     7201 tggaactgcc aggaatggtc cttaaagctg tagaaaattt ccaaggaaaa agctggcggc
     7261 aatcccggca aaaacagaaa gcagtgcacc tgccagcgta taccgtgttt ttgtcacttt
     7321 tgctgccata aaataaacgc tcatcgtata aaataccgtt tccgtgcagc tgagcatcag
     7381 tgatgcgatg attccgatct tggaatccgg cccgaattct ttaaaaatat ccagaagaaa
     7441 tccggtcgcc gcactggcgg aaaacagacg caccactgca agcggcacaa gctgcaccgg
     7501 aaatccgatt ccggcaaaaa gcctgccaag gatatccgag agccatttaa ggaaacccga
     7561 tgcccgcagg atccctacgg ctgtcatcag cccgacaagt gtcggcagga tcgcaaatgc
     7621 cgtgtggatt ccttccccgg caccacgcac aaaatcaccg tatgcatctc tctttttcaa
     7681 aaaagcataa ccgacaacgt aaagcagaac aaacggtatc atatacatcg acaaaaagag
     7741 cagaaatgac agaaaaatcc ctccataagt ccgtccgttt actgtatctt atgaagggat
     7801 ttttgtaact attcaggtaa agcggacatt cacaatccgc tccgctactt gctcatctct
     7861 gctgccagaa acacaaaaac atgcttcagg atttttccac agcagcttcc gcttccatgg
     7921 tttcaagcgt ttctttgatt tctctttctt tttcaacttc tgccacttct tcgaccacat
     7981 cggtttcctc tgtgcctgtc tgtgtctcat tttcctgtgc agtctccgct ttttcttcct
     8041 gtggtttcac aacctttttt gtttcgtttt ctttgcgaag ttctactttt gctttttctg
     8101 cggtttcctg tgcagctgca gcttcttcca caaccgtttc tctgaccggc tcctgctccg
     8161 gatgctgcat tctctgcgga tcactccaca cacgctccca tggatttggc atacgctcac
     8221 cgttcatatg gatccccatg ctctgtgcat acggatacgg cattccccga tagaatcttt
     8281 tttcttcatc agaagcactc ttcatcagtt tgcggcctgc ccataaaaca aatacagcaa
     8341 caacagcaac cggaatcttt gcgatcacag cacccggcag actggtcggc ccgtagataa
     8401 tgtccgcaaa atatttgaca acactccaga tcatgcacgc cgccacgata tacagaaccg
     8461 ttcccatggc ctttttacca tcccgtctgc tgatgctgtc ctttaagcct tcgctccagt
     8521 ccatccagtc atcgccccag agataatgat cctccaattt tttaaacgct tctgcgtcca
     8581 tcgaattctt gttggttgca tcgaaaaagc tgtacagcag aatgatcggt gcaaagaaaa
     8641 gcagcggctt gatatccagc cagataccca ctgcacataa cgttgcaaag attgtcatta
     8701 aagacaatcc ctgcttcata aatcccatat acatctgtcc tgctcccggg atcagagaaa
     8761 acaaaaacat caatatttta ttcttttgtc tcgccatttt cttttccttt cataatgttt
     8821 tcgcatcacc acatctgcat gcgaaacggt atttccatcg aaagcatttt taaatctcat
     8881 tgcaaaattc agaaacactg cttcagcagc tgtaacgcct ctgaaaactt ctccaccgga
     8941 actgctgcac aggaaagagc aagacggatc ccttgctcac tttcatccgc agataaaaaa
     9001 gcaaaacctt tttcttctgc tctgtacaga acttcccgca gatttcctgc tccgaaaatt
     9061 tccagaaggc ttacaaatcc ggtctccccg gccgtaactt ttgcctcttc tacaaaaatc
     9121 cttttcgctt cactgcacaa aatttttgtt ttcgccaaat agtgtttcct ggatttgcga
     9181 atctgacttt ccagatgacc atcccggata aactgacaca atgcgatctg ttccgttttg
     9241 gatgccgtct gattataaaa atctctgcgt ctctgatatt tttccagaag atccggcgga
     9301 agcaccataa aacttaagcg gatcgacggc agcaaaagcc tggaaaatgt tccgagataa
     9361 atgactccgt ttccgcccgc cagaccctga atggacggcg atggatgatg ataataacag
     9421 aattcactgt tgtagtcatc ctcgatcagc aaaatatttt tttcagatgc ttcacgcagt
     9481 atcttcatcc gctctccgac cgaaagaaca tcgccccagg cagtcgcctg cgaaggtgac
     9541 acatagcaga catccggctg ctcctgtttt cgatcgcaca gtttaaaacc atgatcctca
     9601 aaaacggcac gcccctgccg aaatcccgga ttataaaacg caattttttt acgtccctca
     9661 atcaacggac acagaatgtg aagcaggctc tgaacacccg ctccgatcac aatatgttcc
     9721 ggcgtacaaa ccacatttcg gttgttttgc aaatatctgc acagaacctc ccgcagatcg
     9781 tattctccct gcggttctcc ataagagag
//
LOCUS       DAWDWH010000064         9749 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3520_length_9749_cov_14.460078, whole genome shotgun sequence.
ACCESSION   DAWDWH010000064 DAWDWH010000000
VERSION     DAWDWH010000064.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9749)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9749)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9749
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3520_length_9749_cov_14.460078"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    938..947
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2178 a         2631 c         2018 g         2912 t
ORIGIN      
        1 ccgtagcttc caagcatacc aaggtcaaag gaaatatatt tctcatatcc atatttgcgg
       61 atgatctcat acagtttacg caggcgggcg atcgccgcca gagcctgtgt attatcagtc
      121 atactctctg cctgatcgag cacttcgatg tttccaaaca tctgcggaag gcgtttgaac
      181 aggttcgtca gcttttcatt catgttccgc tcttcaagca gtgtctcgac accgaaattg
      241 tttttgttgg tgatcagtct gcgaagttcc tgctcggtag ctttgtcaac accggcctct
      301 gcaagcaggc tctggaagaa ctctacatgt ccgatgctca cctgaaattc ggtaagtccg
      361 gctgccagca gcatctctac gatcattgcc gtgatctcgg aatcggcttc cacgctgccg
      421 tccccgatca gctccgcacc gagctgtgtc atttctttta aatgtccctg aaggcttgag
      481 ttgtttacaa acgtatttcc catgtaagaa agtcggacac ccagttcctc attggaaaaa
      541 tattttgcat aggctctggc gatcgccggt gtcatatctg ggcgaagtac aagggtgttt
      601 ccctcgcggt caaaaaattt atacagatct ttggacggag tcgttccgat ctctctgctg
      661 aatacatcaa aatattcaaa ggtcggtgtc tcaatatcac agtaaccgta accctctacg
      721 actctgtgca gtttctgtaa aagtgctttc tttctggcgt actctccgct gtagatatca
      781 cgcacgccct ccggtgtgtg cagcagtctc tgttccatag gttcctccat tctcttctgc
      841 ccttgctctt tgtgagaata tgcaataatc ccgtcatgta aatatcggca ttaccgtata
      901 aaatcatgca ttagatcctt caaattctcc atctgttnnn nnnnnnngca tccaaatctt
      961 tttgaatcgc ttccagatac ggtacaaata cgccgtgtcc ggcttcaatc tgataggaaa
     1021 gatctacttc ttcaaaacgg aaacttttgc gtcctccatt ctgtgcccgt tcccagaacc
     1081 gtatcatatc cttgaacgga agatataaga tcagatttct tccggtataa tagatcagca
     1141 gaaaggcgat cccgccctgt ttttcaaaat cctgcataaa agcgacctga tgcggatgga
     1201 tattctggag agcaaaggta tctgacgcac attcttttgc atcaaagcag accgggatcc
     1261 cctgcactgc cccgatataa tcgaccgtac ttttctgatc aaaatacgcc agcgtgatat
     1321 gacgcttttc tttgtcgatc ttgatcggcg tgatcggagt cgggatcttc tggatcagtg
     1381 ccagcccttt ctcgcgatac tgttcgttcg tgcggttgat cagttcctca agtgcggacc
     1441 ctcttaaacc tctggaattc catgttccca tgttccaagt cctcttttct gtgcaatact
     1501 cacaaactgc ttcggtgccg gagcgtagat gcttttaccg gaaagtcctt caaaatcacc
     1561 ggaaagctct ggaaatacca gatgccaggc atgcagaagc tgcatgtgaa gtccgtaccc
     1621 ggaagtcagt tttttattca acactgcgtc tccgtatttc ggatcaccga agatcggatg
     1681 tcccatatag gacaaatggg cacgaatctg atgcgtacgt cctgtcagaa gctcgatctc
     1741 cagaagcgtc tcctgcccat tgtctgcaag cggacggtat tttgtgtgga tcggtctggc
     1801 atcctttgtc tcgtttttga aaattctgac ctggttggtc ttttcgtttt tcagaagcca
     1861 tcctctgaca tcctgttctt ctttgatcac gccttttacg atacaaaggt aatatttgtg
     1921 cagggtacgt tctttgaaaa gcccggagag catctgcaaa ccctgcagcg tcttgcccgc
     1981 tgctacgatt ccgctcgtat tgcggtccag acggttgcac agggacggcc ggaatgtttt
     2041 taacatttct tcggtcactt cgccggtatc cagaagatgg gaaatcagat gctccaccat
     2101 ggaaatatcg cctttttccg ccttctgcga gagcatccct gccggcttgt tgatcagcat
     2161 gacctgctca tcttcataga gaatatccag ctttgccccg gtatgtacgg ctcttttttt
     2221 cttttcccgg aatccttcga tcgtctcttc ggagagaaac agtttcactg tatctccctt
     2281 ttccagtttt tcatttcctg ctgctttttt accgttcagc gtgatgtttt tctttcgcat
     2341 catcttatag aaaaagcttt tcggtgccag ttccatatat tttgcgagaa atttatcaaa
     2401 acgctgccct gcctcgtact ttgagatcac aagttctctc attgcttttc ctttcccggt
     2461 attttactgt tgtcaggcgg atattcgcaa tcctctttgc tgcctgattt tgttaaagta
     2521 cgatcgcacg cagcacgtcg cagatatatt ctgccttccg gctgcccgtc gtctcatgtt
     2581 cactctgttt ctggttcatc tgctttaact gatccagaaa acgtttttcc tcccggaaca
     2641 gtttcacacc gaccgatttg catttattgt tctttaaaat ctcccggatg tgttcttctc
     2701 caaacgaaag atccgctttt tcactgctca cataaccgat cacctgcgtc ccgctcacgg
     2761 caactgcatc aaccggaact gttttctgat aagcagtaaa ggtcatatca tataagatcc
     2821 gtacattttc gtttacctgc tcgatctttt cttttaatgc ttccgatgcc ggtttttgca
     2881 tcagcatcat gataaacccg gttgctgctc tggctgccgg gatacacccc atgatcgcca
     2941 caaaggtaaa catattcagc cttgtctttg tgataaaaag cccggtcaga aaaatgatga
     3001 tcgggattgc aaacatcacc agcgtgatca gtccgcgttt gattttctgg gatttgatat
     3061 acccaaattc tcctttgtct ctcttcatga tttcctccag ttgtttccga ttcacagtcg
     3121 gtcctttcac agagtctggc tgccggttgc gttatggttt aaaaaatatg gcagtcaggt
     3181 cgatgatcag acggtgcggc aaaatcttgc acgcaagaaa caacgctttc atcgaccatc
     3241 cgtaaatgga caatgcccgc ccgcaggccg catcataaac ggccttttcc acgacaggct
     3301 ttggatctgc catagatttt ttcttaaaca gcggcatttt atggtatttc tcggctcctt
     3361 caaaaaaagc cgtgtccacc ggtcccgggc acacacaggt cacggtgata cctttttttc
     3421 gcacttcgct tcgcagtgct ctggaaaagc tcagcacata agcttttgat gctgcataga
     3481 cggcaaatcc cggctgcggc acaaacgccg atgccgatgc catctgaatg atatggctgc
     3541 ctttctccat ataaggaagg cacagatacg tgacggtcat cagtgcttcg tcgttcagcc
     3601 tcgtcgtctg ctgatgctcc ggaagtgtga tctcttcaaa tgttccgatc cgtcccattc
     3661 cggcggcatt taccaaaatc ttgacccgga catcctccgc aaagagcctg catcttaacc
     3721 gctcctgctg tgcttcctgc aaaagatcca gcggcagaag cacaatcttt gtcccaaaac
     3781 gttcctgcca catctctttt tgcagatgcc ttgcaatcac ccagatttca tcgagtctgc
     3841 ggtacttgtc catgatctgc tgtacaaatg ctcttcccat accggaagag gctcccgtta
     3901 caatggcaat cttttttctt tctgacatat cacttcttct ttctgttatg cgtgttgcgg
     3961 attgtcttct ttttgcctgt cggacgctct tttgcagtat ttcgtccatt ttgattgctg
     4021 ctgtttcctg tatttttcct gcctgcattt ttgccaccca tgtttctgtc atcttgtccc
     4081 cggcgatttt tatccgtgcc ctgataattt tgacgatttt tatccggacg cgggcggatc
     4141 agacatttgg cgtcaaatcc gatcaggtcg ctgcgatgtg cctttaacag tgcctctttg
     4201 accagctcat aattggccgg attgcggtac tggatcagag ctctctgcat cgctttctca
     4261 tgcggatttt tcggaacgta gaccggctcc atcgtccgcg gatcgacacc agtatagtac
     4321 atacaggtcg agatcgtgga cggtgtcgga taaaagtcct gcacctgctc cggcatatag
     4381 ccaagatcac ggagatattc tgccagttcc acagcttctg tcagggaaca gcccgggtgg
     4441 gaggacatca gatacggcac cagaaactgg ttcataccct cctgtctgtt gagctgttca
     4501 tattttttaa caaatttctg ataaacagca tgctccggtt tgcccatctt tgcgagcact
     4561 ttgtctgcca catgttccgg agccactttg agctgtccgc tgatatggta tttgaccagt
     4621 tcccggaaga agctctcatc cttatctgcc atcaaataat caaagcggat accggaacgg
     4681 ataaacactt tcttgacttt cgggatcttc cgcagtttac gcagcaggat cagataatct
     4741 ttgtggtctg cccgcatatt ctggcacggc tttggaaaca gacattgttt gttcgggcac
     4801 acgccctttt tcagctgttt ttcgcatgcc ggatggcgaa agttggcggt cggtccgccg
     4861 acatcgtgga tatagccttt gaaatcctta tcttccgtca tctgtttcgc ttcttcgata
     4921 atggactcat gccctctcgt ctggataatc cgtccctgat ggaacgtcag tgcacagaaa
     4981 ctgcatccgc caaaacagcc gcggttgctg accaggctga atttgacttc cgagattgcc
     5041 ggaacgccgc ccctggcatc gtaggacgga tgccaggttc ttttataagg caatgcatag
     5101 acatcatcca tttccatctg cgtaagtggt tttgctgccg gattctgcac aatataaaga
     5161 tgctctccgt aaggctccgc aagccgcttt ccggcaaatg cgtcggtatt acagtactgt
     5221 gtataaaaac ttctggcata ccagagtttg tcttctttta atttttcata ggacggcagc
     5281 acgatcgtat cgacggcttc atccagcatc cttgttttgt aaacggttcc cgcaataaac
     5341 gtgatatcgc gtacatggat tcccgcatcc agtgcttccg caatctctat gatgctgtgt
     5401 tctcccatcc cataagagat cagatctgcc cccgaatcca gcaaaatgga gcgtttcagg
     5461 ctgtccgacc agtaatcata gtgggcgagt ctgcgaagac tcgcctcgat cccgccaatg
     5521 atgatcgggg tatgcttgta ggtgcggcgg atcaggttgc agtagacgat cgtagcattg
     5581 tccgggcgtt ttcccatcac accgcccggt gtgtaagcat ctttctgtct tcttttcctg
     5641 gaaaccgtat aatggttgac catggaatcc atgtttccgg ccgacaccag aaatccaaga
     5701 cgcggcttcc ctaaaatgga aatactcttt tcatccttcc agtcgggctg tgaaatgatc
     5761 cccacccgga agccatgtgc ctccagaagc cgactgatga tggcatgtcc gaacgacgga
     5821 tgatccacat aggcatctcc gatcacatag acaaaatcca gctgctcgat ccctctctct
     5881 tccatatctt ttctggaaac aggcaaaaaa tctgtgctca ttctttttcc tttcttggcg
     5941 gctgtaaaag tgccgcaatc tcttcttcgg tcagtttgcg gaacgctcct ttttccagag
     6001 ccggatctaa ggtaaggctt cccatggaaa gtcgtttcag atagatcact ctgcagccga
     6061 tcgcctcaaa catccgtttg acctggtggt aacgtccttc gcagatcgtg actttcgctt
     6121 cggaaatctc tcccgacgag aggatctcaa ggtccgccgg aagagtcttc ttttcatctc
     6181 cgatatccaa cccttccttg aatctgtgga tatcttcttc atggatctgc ccggtcactc
     6241 tcgtaaaata gcatttatcc acatgttttt tcggggaaag cagacggtgt gccagctctc
     6301 cgtcgtttgt gacaagaagc agtccttccg tgtctttgtc cagccttccg accgggaaca
     6361 gatccttttt ctgctgcaaa gtaattagat ccatgacggt cttttctttt ttatcttcgg
     6421 ttgcggtgac cacacctgcc ggtttgtgca acatcagata gcaatgctct tcataatata
     6481 ctggttttcc gtttagcgtg atctgctcgt tccccagtac ttttctcttt gcctgctttt
     6541 cggtgatacc gtccacctgg acaagtccct ttttcaggaa ttgtttcacc tgggagcgac
     6601 ttccttcgcc ggcaagtgtc agatactgat ccaatcgata actcatgact gcattctcca
     6661 tcctgcataa tacttgtttt ttaaggtacc gccggccgct ttgccccagc ccagcccgta
     6721 accgtccacg cagaccagta cccagccttt ttcgttttcc gcctctgtgt cggataaatc
     6781 cagggtctct ccttttaagt aacggatcac acgctcatcg tcccctttaa aagagacgct
     6841 ctttttaaaa tcttctgctt ttaagcacat ggcaagagcc tgcgacggct caaaccgctt
     6901 tttcttctga tctccaagat aaagcccggt gcgaagataa cggatacccc gctcgatctt
     6961 cgtcccttcc ggaagcagat agatgcgttc tttctgaact tccgtttttc cggataaagg
     7021 ctttgttatc tgctgcataa atgctgcaaa ttccgggcag tccttgaggt cagacaccgg
     7081 tgttttttta cttttcgccg gttttttcag atatttttta ctctgtcctt cgccgtcggt
     7141 gcgttttcca agcagagccg caaaatggcc ttcgcccggt gccagatgcg ggaaaatgcg
     7201 gatacagttc tcaaatcctt ctttttttcc atgggagaaa tattcatccc attctggcat
     7261 cagaagttcc atatccggac gctcctgcaa aagataagcg atgttttctt cattctcttt
     7321 tacagaaaat gtacaggtcg aatacaggag cattccgccc tttttcagca tatccgccgc
     7381 atcgagcagc agctttctct ggatcggcac atagtaatcc ggtccttttt gcagccagtc
     7441 ttttaccatt gccttatccc ggcggaacat cccttctccg gaacacggtg catcgatcag
     7501 gatcttgtca aaatacgccg gataagcttt tttgagctta tcggactctt cacttgatac
     7561 aaacagattc gaaatgccaa aaagctccag attctttaaa agccctttgg ctctggaatt
     7621 gctgatgtca tttgcaagca gcatgccttt tccttttaat tttgcaccaa gctccgtcgc
     7681 cttgccgccc ggagccgcac agagatccag cacaaaatcg cccggctcta ccggaaggtg
     7741 tgatgccggg atcatcgcac ttggctcctg cacataataa agccccgcat aataatatgg
     7801 atgctttgtc accgtctgct tttcatcaaa agcaaagccg ttctcacacc acggtacaca
     7861 ttcaagagaa atatctgcct tctgcctgaa ggtttccggt gcgatcttcg cggtatttac
     7921 acgaagtccc tgtctggttt tctgctcaaa acttgcaaga tatgcctcat actgatcacc
     7981 gagcagttct ttcatctctt ctaaataatt tgaaggcagt tccatctttt tatcctttct
     8041 cgtttcttat tatttgcttt tcctatacca cgtctgctca aaaatgcctc tgacattctt
     8101 tatgcatcaa gatcactcaa atgcctgcac atgataacct tctgtgatat tgtccagatc
     8161 gctgtgaaaa cgacgcagct gttccaaatc ctttgtctca tagatccggt aaaactcatc
     8221 ctggatcttg acaagttccc gtttattggc aatcgtatac agattctgga tattatttaa
     8281 aatatctttg accagattcg tctggatctg gcaggaattc tgtgcatcca tgatctcgct
     8341 gcgtaccgtt gacatctcat tgtccagcac aatgatcgca tcggcaacgc ggctcagttt
     8401 ctcccggatg tgatccggca tatgctgttt atatttatac tgaagcgaat gttcgatcgt
     8461 agaccagaaa ttcatcgcca gagtacggat ctggatctcc acgcggatgg tctttgttcc
     8521 ggaaaaggtc tccaccgggt aagacacgat catatggtaa ctgcggtaac cgctcgattt
     8581 catatgattg atatagtcct tttccgtaac gacctgcata tcggagcggt ttcttataag
     8641 atccgccacc ttctcaatat cctccacaaa ctggcagatg atgcggatgc ctgcgatatc
     8701 atccagctgt tcttcaatct tgtcaacttc aatgtttttc ttctgtgctt tatctaaaat
     8761 actggagatc gatttgactc tgccgcagac ctgctccatc ggcgaataca taccccgatc
     8821 ctggtactct ttgatcagat gattaaattt taccagcagt tcctccaccg ccagctcata
     8881 tggatttaaa atatccttcc ataactgtat ttccataaaa accactcctc ctacattact
     8941 taccgtcctt cctcagtcag accagtatag cataattttt ccttttttcc aactgtcacc
     9001 tcaaggttct ccctccagac cttttaagat ccagtaagga tcgacactga cttcccgccc
     9061 gtctgccgtc tgtatgtaaa tgccaaaatg cagatgtacg gcaaactgac cgactgtgcc
     9121 ctcttctccg taaccgctgt cccccatata gcccagcatt tcgccggctt ctactgtttt
     9181 cccttcttcc atccccggtg catattccga gagatgggca taataaaagt aaccgccctg
     9241 tttactgcgg attccgatcc gccatccgcc cttttgaagc catcccatat gctcaaccgt
     9301 cccggctgtg gcagaaaata ccggataaaa ccctctctga tttgtcggcg gcatcagatc
     9361 cgtcccctca tgcccccgtt tgccgccgta ggtgcgttct tccatccagg aatcctcata
     9421 agataccctg cattttactg gaaagcagac aagatcatcc cataccgcac agaacgcatt
     9481 ttccagcatc tcatattgtt tcgggcgata acggtacatc cacttcgccg tctgctgcaa
     9541 ctcatcgatc gtcggcattt tctttggaaa agacgcctga gcacccgcaa aagcaagtgc
     9601 cagcagcttt cctttcgaaa gtccgctctg tttctgaagt tctgctgttt cttcctgcaa
     9661 gctgccgcct gcctgcatag aacggaaatg ttccatttca atgactctgt catgcagagc
     9721 atattttctc ttttcatgat ggaatatcg
//
LOCUS       DAWDWH010000065         9357 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3673_length_9357_cov_8.884541, whole genome shotgun sequence.
ACCESSION   DAWDWH010000065 DAWDWH010000000
VERSION     DAWDWH010000065.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9357)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3673_length_9357_cov_8.884541"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2875..2884
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4246..4255
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9134..9143
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2305 a         2438 c         1804 g         2780 t
ORIGIN      
        1 tcatcggctg ctccagattt ttgccgtaag aattgacatt ctgtcccagc agcatcactt
       61 ccacaacgcc atctgcaacc agttttttaa tctcacgaat gatatccttc ggctcacggc
      121 ttctctcacg tcctctgaca tatggaacga tacagtaact gcagaaattg ttgcagccaa
      181 acataatatt gacaccggat ttgaacggat attttcgctc caccggaagc tcctctacaa
      241 tctgcgtggt atctttccag atatcgatca ccatacgttc agagatcagt ctggtgtaga
      301 tcagctctgc aaacttaaaa atattgtgtg ttccaaaaat cagatccacg aaacggtagc
      361 tctttttcag tttttctaca acttccggct cctgcatcat acagccgcac agaccgatca
      421 gcatatgcgg atttttcttt ttcatgctgt ttaaagctcc gagtcgtccg taaactcgct
      481 gatttgcatt ctcacggacc gtacaggtat tgtagatcac aaaatctgca tgttcgtctt
      541 ctgtcattac ataaccgatc ttctccagaa taccgatcag cttttcactg tccctcgcat
      601 tcatctgaca gccaaaggtc accgtgcacg cagtcagcgg acgtcccagt tcttctgctt
      661 tctgtttcag gatggcacgg gcttttttca taaaataata ctgtctctgc ggttcttcct
      721 gtggctccgg accattctcg tcaatgttct ctaatatctt ttcgaattct tcgttgtttg
      781 tcactaattt tcttcccttc tataattgca ataacttttt ctaatataac atacgatatt
      841 gcaaaaagca acggaaaggt ctgctatact cttctgattt gccgtgctcc agtctgccag
      901 cttctgaaag aagtacatcg gcagaaaaat gattgctgct gccagaacca atcttccaat
      961 atcaatatca agatccatat tacattcccc ttccttaata ttttttcaaa tgccagacac
     1021 tgtttcttga aaatattcct atcttcctga ttacaaatca tatttcttaa aaatatctcc
     1081 cgtcaattca actgcttttt ccgttaaatt ttctcagcgt ataacaaacg aacatctgtt
     1141 aaacagatgc ccgtttgtta tatcatgctt tacttttatc tgattactgc tcagtatatt
     1201 ttctgtcttt ccagataaga taccatgcga tttttgccaa tccaatggca atgaaaaata
     1261 tacctaattt tcttaacata tctttctatt cctccgctct tgcttccagg tattggcatt
     1321 atctattccc gttatctttt aacgcacctg accgtttcca tagataatgt acttcgtggt
     1381 cgtcagtgcc gtaagcccca tcggtcctct tgcatggagt ttctgtgtac tgatcccgat
     1441 ctcagcccca aagccaaatt caaagccatc ggtaaatctt gtggaggcat tgacatagac
     1501 agctgctgca tccacttctt caagaaattt ctgagcattt gcataatctc ttgtgatgat
     1561 cgcctcggaa tgtccggtgt tatagcggtt gatatgtgcg attgcttcgt cgatggaaga
     1621 aacggtctta acggagagga tataatcgag atattctttt ccccagtcac tctccacagc
     1681 cggaacgatg ccggaaacgc tttcctgtac ctgttcatcg ccacggatct caactgattt
     1741 ttcatccagt tttgccttta acagcggcat cagtgctttt tccacttttt catgtaccac
     1801 cagtgattca caggcattgc agaccccgat ccgctgtgtc tttgcattga agatgatatt
     1861 gacggccatg tcaagatctg cactttcatc gacataaata tggcagttcc ctgttccggt
     1921 ctcgatcacc ggaacggtac tgttctccac aacagcacgg atcagtcccg caccgcctct
     1981 tgggatcaga acatccagaa aatctttccg cttcatcaat gccgttgtga cggcacggtc
     2041 ggtatcctcg atcagctgga tcgcatcctc atccactttg cacgctctta aagcttcacg
     2101 gatcactttt acaatggcaa tgttggaatg catcgcatcg cttccgcctt tcaacacgac
     2161 cacatttcct gttttaaagc agagtccgaa ggcatctgcc gtcacattcg gtctggattc
     2221 gtaaataatg ccaaccacgc caagcggaac acgtttctga ccgatcagca gtccgttcgg
     2281 gcgttttttc atagaaagca cttccccgat cgggtcttcc agccctgcga tctgtatgag
     2341 tccttctgcc atctgggcaa tgcggtcttt ggtcagcagc agacggtcca gaagtccctg
     2401 atgcatcccg gcttcttttc cggcctgcat atctttttca ttttctgcga tcagataatc
     2461 tgctttctca ttcaatgcat ttgcaactgc ttccagtgcc tgattctttt ttgttgtccc
     2521 aagcaactgc atatgcggct tcgcagccat cgcatacatt ccaatctcgt ccagtgttcg
     2581 cttcattgac aaatcctcct ttttattttc cataaatcat cgttggtgtg atcagcatct
     2641 gcgggcagat atcatacaca tctgacggaa ccgtttctac gatctgaaaa tccagtgcca
     2701 tggcaacggt agcatgctgt gtatgtctgc ttaaatagcg gtcataaaat ccctttccat
     2761 atccgcagcg gtggcgatct ttatcaaacg ccacacccgg caccagaagc agtgccgtct
     2821 catctgccgc ttcttccaga tttctcgtct cgtccggttc cggtatatta aaatnnnnnn
     2881 nnnncatcgc gttcttttgt ctcggcactt gtcagtcctt ctgcttttgc ttttctggca
     2941 agttcattga ttcttgctaa tttttcatcc gttatcatac tatatttctc cttgttttat
     3001 gatgtgggta tcaaaagtcc ccaactatga tgccagagtc aacttgcaca ttcccggcat
     3061 tataacattt tttccagata atccatcaaa taaaactcat cttttttgtg agaaagaaaa
     3121 agggtaccgc agtcctgtcc ttcgatcaca cgatggatat ttcggaaatc ttctccattt
     3181 gcaatgacca tatccgtgcc tgctgcggaa gcgagctttg ccgctgtcaa tttcgatgcc
     3241 ataccgccgg tgccgacatc gcttgtagat gtgctctttc ccattttcag caaatggtca
     3301 tccagttttt ctactacatg gattaattcc gcatttggat tgctgttcgg atcatccgta
     3361 aacagaccgt caatatccga cagcaagagc agcagatctg catcgatcaa tgccgctacg
     3421 atcgcagaaa gcgtatcatt atcaccaaac tgcatctcat acgtggaaac cgtatcgttt
     3481 tcatttacaa tcggaatcgc ccctaatttt aacagttccc gaaaggtatt cagtgcatta
     3541 aaacgactca gattgtccat aacggtacgt ttggtcatca ggatctgtgc tgccagctga
     3601 ttgtattccg aaaaaagctt ctgatagatc atcatcagtc tggcctgtcc gattgcagca
     3661 catgcctgtt tttcttttgt ctcacttggt cggtgtccga gacctgcagc tcttcttccg
     3721 accgcaatcg cccccgatga taccacaatc acatctttgc ccatattatg gagatcactg
     3781 atctcacgca ccagaacttc cagtttcgta aggttcaacg cccctgtctc cgggtgagtc
     3841 agggacgaag aaccaatctt tatcacaatt cgctttttat cttttaatat ttctctgata
     3901 ctgctgttca tgttttcctc ctgaagtatc atccatctgc tgctcgtctg cagatcattt
     3961 tccatgctat cttacaccat tttgtcaggt acgtccagag gtcaattgcc cggataaaac
     4021 aggaaaaagc ggaaatactg aaaaaaagga gatgaaaaag atcatgaaac atcggcctat
     4081 ttgttttgcg gcagcatttc tcttacttct tctgatttgc accgcctaca atttaagcta
     4141 tcactatatc accagaaatc cgccttccgc cgaagaatct gctgatcatg aaagtgaaac
     4201 catacgctct gctccggcaa atgccgggaa tccgcatccg gtatannnnn nnnnnttaca
     4261 gaagaacagg gctgcgtctc aatttatctg gatgataaag aaacgttgta cgaaaacacc
     4321 agcatccgct taagcagcct gccggacgct ctgaaaacag aaattagaaa cggaaaatat
     4381 atccggaccg aaaaagaact ttatgatttt ctggaaaatt acagcagtta gaaaacaggt
     4441 cgtgaacatc aaagctcacg gcctgttttt acacatgact gctattacag tatattcagc
     4501 cgaatcagaa gatctgccag cagatcacct tttggaagtc tgcggatctc acgctccagg
     4561 ataccgccaa gttttacaag acagatacca tagacaacag ctcccacgca taccgccagg
     4621 atcgttgaga tcgtatttcc ggcaaacaga ttgaacactc tgtgtaccag atatgcggca
     4681 atgcccatca caccggccgc gatcaccgga accaggaagg tctttttaat ctcctggcga
     4741 tagtgcagtt ttttgcggat ggaacgtgca ttgagcagac agatgatcag tccgaaaata
     4801 atgtttgcat agactactcc ataaatgttc catttaaact ttacaacaaa cagaactaaa
     4861 aatccaagat ggatcgccac ggctgtcgct gcatgcacaa gcggtgcctg catctcacca
     4921 agtccctgta aaataccggt ggtaagcgta gacagtgcca gaaggacggt cagaagtgct
     4981 ccgctttgca taatgcctgc cgcaagggcc agactgtcgt tgtttccgta gagcatcatc
     5041 atgattggtc ttgccagtgc cgccataccg actgcacacg ggattgtaaa taccatggta
     5101 tagcggatcg tatcgcgggt ccgaagtccg gcttcccgta aatcattgtt cgccatcgcc
     5161 tctgtcagag ccggtacaac cgaaggtgcc agacaggatg cgatcgacag cgggacgttc
     5221 atcaacacgc ggaatttacc gctgtagatg ccccagacgg ttgcatactg tttctgggta
     5281 aatcccatgc ctgccagaat gtggttgaac acgccctggt cgatgatcgt gctgatatta
     5341 tataccagcg tactgagcac gattggaaga atagtgtaag taatcgcacg gtaaatggca
     5401 cggtcagatt ctcttcttct tgttttgtcc tttttcatgc ggcgtttgaa accgttacga
     5461 tagccaagat agatcactat cataaacagc agtgccacac cgacgctggc gaccgttcca
     5521 aatgtacctc ctgctgctgc aagtgcggtt ccaagctccg catcgccttt tttctcacca
     5581 agcttcaggc cgtactgcag catcacataa gcaccggcta ccgaaacgat ggcattgatg
     5641 atctgctcaa tgatctgcga caccgctgtc gggaccatcg taccgtgtcc ctggaagaat
     5701 ccacgcagta caccggtcag tgcaaaaaga aagatcgccg gtgcaaggac acgaagtgca
     5761 tagcttgcaa gctccacccc catcacatac ttggtgatca caccgccaag caggaaagtc
     5821 agaatggaaa gaataccgcc tgtcacggcc gcaaaacgca gggaacagat cagaaccttg
     5881 taggaatttt tcacttctcc tgcatactcc cgctctgcta ccagcctgga tacagcaagc
     5941 ggaaggctga aagaagaaat catcaggata atagtataaa tctcatttgc ggtagaataa
     6001 taaccgtgtg agtgagcgga atacggtaga tcataccgat tgcttttgcg acaaaggagg
     6061 cgattacaag aatcgtcccc tgtgccagaa aatttgtttt gcttgaactc tttttactca
     6121 tattttatca tacctctttg ttcccgcggg cagcgaacca ggcagacgca cctgcgacgg
     6181 ggtatatttt ctgtattttt ttattcgatc acgatctggt cttcttttgt atcataaaca
     6241 acacataccc aggtctttcc ctgattgatg gtaatctcat ttccatcttc atcaaaataa
     6301 cgtgccggac tctcaatatc atccttggtc caggtacatt tttcgtaagt tccctttgtg
     6361 aagacatacg cggtaccacc tttttctgcc tcaatggcaa ggtatccgtg atcatccagc
     6421 ggataacatt cgcaaagctg gaaaatgaca ttatcgtatt tcagctgatc tccggtcagc
     6481 tgatcgatct gtgcgtcgcc atactgataa cgatagtata caccttcatc tgcattatag
     6541 tcaaaccacg gttcatttac ttcatatccc ggttcaatat gggttgccgg atctgtaaat
     6601 gtgacggtct gatcgtcgcc cgcaaactga taatgaccat cgtaatcctt atcatactct
     6661 cggctgtaac acagtttttt tgtcgcttcg atcagacggt cggcactggt ataacagttg
     6721 tgcggagacg gtctgtcatc cgtgcggtaa aacgtctctt ctccggcata tccataaagc
     6781 tgtccaaatc cctcctgtga agaaagtccg ctgatgttgt caatgttgct gttcagaatc
     6841 tcacacgcat aaacagcctg tccgtaatgt acatacaatg catcaaattc ttttgcaaaa
     6901 tgcacataat actcacggca gcttcgaaca gaaccaatct tctccatccc gtcgtagtct
     6961 tcaaaaatac ccatcagtct tgtgatcgca ccttctaccg gagcttcgta taccacatcg
     7021 gcacgttcga taccggcctg cggaatggca ccgtaaatgt tattgagcat gatcgcatac
     7081 ggtcttttcc gaccgatctt tttatcaacg atctttccgg tcaggtagct gcgtactttt
     7141 cctttcggca gtgcctctcc ctgatcaaac gttcccgctt cggttgcaac cgtttcggtt
     7201 tccgtttcag tctgcgtttc cgtctgtgtc tgtgtctcgg tttccggtgc tttctgtgca
     7261 cagcctccaa gtacggcact tgccatcagt acagccgcca gaactgctaa tttctttttc
     7321 attctttctt ccccctatct tgtaattccc atggattcca ggacatctgc ctgtttcttt
     7381 tccatacgtt ttgcttcctg ctcttccata tgatcgtatc cgaccagatg cagaagactg
     7441 tgtacgatca aaaaagcata ctccctaagc ggagaatggc cgtagctttc tgcctgctgc
     7501 aacaccttgt caccggatat cacgatatcg ccaagcaata gttctcccgt ttccggatga
     7561 aaacagtctg ccccttcctc ctccagaaat gcaaaatcac cttctgtgac atagtctgtc
     7621 atcggaaaag acagcacatc agtggcacgg tcaatatcac gctgttcttt gttgatctgg
     7681 tggatggtct catcatcggt cagcagaagg cttacttctg cctcataagg acagccaaga
     7741 taatccatcg taaatgtaaa taccttttcc gccacttccc tggcatcaaa cggcagctct
     7801 ttttcgtact cgctctcaaa attcagtgtc atgattttct ccttgttgta cccgtcgaag
     7861 tcttcgtttt gctctttacc gttctgtctg tctttttctg tacagctttc ttttcgtatt
     7921 cctcataggc ctgcacaatc ttctgcacca gcggatgacg tacaacgtct ttgctggtca
     7981 gttcacagaa cgaaatccca tctacttttt tcaggacttt catggctaca tccagtccgg
     8041 aagcggcact ggatggaagg tctttctgcg ttttgtcacc agtgatgata acttttgagc
     8101 caaaaccgat tcttgttaag aacattttca tctgtgccgg cgtcgtattc tgtgcctcat
     8161 ccagaatgat aaaggcattg tccagagtcc ttccccgcat ataggcaagc ggtgctactt
     8221 caatcaatcc tttttccatg tttttggcaa aactttccgg tcccatgatc tgatacagtg
     8281 catcatagag cggacgcaaa tacggatcaa tcttgctttg cagatccccc ggcagaaatc
     8341 caagcttttc tcctgcctcg atcgctggcc tggtcagaat gatcctgccg acctcatcgt
     8401 ttttaaatgc ctggatcgcc atcgccatgg ccagataagt ctttccggtt ccggcaggac
     8461 cgatcccgaa aacaatcatc tttttgcgga ttgcatcaac atactgcttc tgtccaagcg
     8521 tctttggttt aatcggcttt ccgttgatcg tatggcagat cgtatcctgg tcgatcgcag
     8581 aaagtgcttc ttctttatcc tcataagaaa gtgcgatcgc atagtcaaca ttctgttcct
     8641 gaataatatt tccatgcttt gacagttcga tcagctgttc aaaaatcttc ctggctttgt
     8701 tcactgccgt ctccccgccg atgatcttta aaacgccatc acggttcaca acagtgatat
     8761 tcagtgcccg ctccatcttt tttatatgct ggtcaaattg gatgcatctc tgtcaccgta
     8821 tcaaacgtct gcattttttt gccggcaccc ggcagataca tctgccattt tccgactcga
     8881 agtccgatct tttgtttctc ttttccgctg taatgcttct cttttctctt taatcgtatc
     8941 ttttcccgat attcataaga agttttaaga aaaatatctg catccgaagc acagtactga
     9001 taaccggata cttccccgga atcatccagg atctccagct ctcctttcac aagcaagtca
     9061 ccctttttca ccgtgtcacc gactgacacc attggtactc cttttcttgt gatcatggat
     9121 gttatgacac cgtnnnnnnn nnnaataaca gacccttttg cgatgcaata gtttgtatca
     9181 aactctattt taacattatt ttcaaatatt tgaacccctt tttcctgaat tttattcaga
     9241 aaatcataca gatctttttc agccatggca gatgcttctt cttctgtcaa tgtcaccgtt
     9301 tcgtatgtat actcgaaaaa ctgctgcttt tcaaaagaaa ccggaagata aaaattc
//
LOCUS       DAWDWH010000066         9347 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3677_length_9347_cov_9.279488, whole genome shotgun sequence.
ACCESSION   DAWDWH010000066 DAWDWH010000000
VERSION     DAWDWH010000066.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9347)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9347)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9347
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3677_length_9347_cov_9.279488"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2491..2500
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3348..3357
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4106..4115
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7484..7493
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8529..8538
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2096 a         2215 c         1779 g         3207 t
ORIGIN      
        1 gtgtcaggct ttccagggtt gctttgtctg catctgcagg agcatttttc aatgccttgt
       61 aagctgcatc aaatgctttc cagcttgctt cttcataatc tttctgtcct gcatctgcaa
      121 tcgccgctgc gtctttcagt gtgttggctg cgttttcttt tgctttttcc agatcagctg
      181 tttcctgaag taccagagca tcttttgcat cattcagatc tttgattgcc ttttcaagtt
      241 ctgctacggt tactccgtct ttgcccggaa catctttcac tgctttatat actgtcactg
      301 cattattata agatgctact gtatatttgt taccatttgc catcagatca tcataaacag
      361 ctgtcagcag actgttcagt tcttctttct tagcagcaac tttggtcttc agacctttct
      421 tcgcatcgct gattgctttg attgcgtctt caatttcggc cttggataca tctgtcttgt
      481 ctttcagtgt atttgctttt gcaagagcat tcttcaaatc cgcatagctt tcttctgtat
      541 aatcagcttc ttttaaatct tttacagaag cgatttctgc tgccagcttt gctttatctt
      601 ctgcggatgc tttgtttgtt gtaacagtca gtgtgatctc ttttgtgctt gtcttggttg
      661 catcagatgc cagagttgct gttactttta cttttgctgt acctgctgct gtcactgtca
      721 ctgcattgtc ttctacagtt gcacctgttg tacctgcgtc tgttactgtg taggtatagt
      781 ttacagttcc gtcatgtcct tctaccttgc agtcaccggt tacctgtgct tttgcactca
      841 gtgttactgt cttgctgtct gctacgccaa gagcaatcgt ctgatctgca acacctgtga
      901 tctcgccaac tacacaggag caaacaattt ttgcagttct cgggaattcc ttggtaacag
      961 tcatggtttt tccgtctgct gaaatcgtaa cggttgcacc gtctacgctt ggtttgctga
     1021 tgtcagtaaa tttgaatccg gaaggagccg tgtatacggt tgttgccgtg tatacagtct
     1081 ctcctgcaaa cttggcatct ttttcaagag cagtaccgtt tgctgaccat gttgtcactg
     1141 ctgtgtatgg aattgcagaa atatcagcat ctggtgccac cttttccaga gcggccttga
     1201 tctcatcata agattttgtc agaccttctg aataatcggt gcctttgtaa agtttgatat
     1261 ctgcaatctt accatctacc tgatccagtg tggagtattt aacaccgttt ccatctgcct
     1321 cgtggtttgt actcttttca gtattgtatc cgattgtaaa gatactggat gcataagatt
     1381 taaatacaac ctggtatcca tctgatgcat ttactcttcc cggtgtagct tcaccagctt
     1441 ttccatctac ataaagctgc atatttgttc cggtatatac cagtgcgata tcatgccact
     1501 ctccccagaa ggtatcggca tcatatttat actgtacctc cggccattta tcttgtgcat
     1561 cgcagcagta caggatcacg cggtcatttg caccgtctac ctggatacca tactgagaat
     1621 ccatcttacc aaggatattt acaacttcat cagttttatt gttcagtttt acttttgtgc
     1681 gaagaaccat cggtgtatcg ccggatacat caaatttatc attgtttgct ccgttgttcg
     1741 gtgcagtcag tcttccctgg aatgccaata cgccgccatc tttaacgacc gttgtcggaa
     1801 cgtcatcttt cacttccagt gtagccggtt tcgcagactg gtcttccatt gcaacatagc
     1861 ttggtactac tgcggtttct ccggctaccg gtgctgtata agaaagcctc ggtgctgtga
     1921 tcacagaatc ttcggtaact ggcggagccg gtgtttcagc ttttttcagt gctttgatcg
     1981 catttgccag tgtatctgcg attgtctgaa catcctcatc ttttgtctcg tcggtaacag
     2041 cgtttgctgc atcccaggct tctttgaatg tcttgtaaga atcctcggta tatttatcag
     2101 cagattctac tttttctgca tctgcaagtg ctgctgccag agctttgcgt gcctcggata
     2161 cttctggtgt ttctgtatct tcttttccga ttacgccaaa ttctgctgca ttgataaaca
     2221 tattggcctg aacaccagct gtcttgatcg ctttcacctt aacgtctgta acacctgcat
     2281 atgttttgtc aaaagttacg gtcctttcaa cgaagttctg gtctgcacca tcttctgttt
     2341 ctttcacata attccatgcg gtttcgcctt ctgcttttcc tgccagagtc cagtttttac
     2401 catgatcggt agatacataa acttcacatt cagaaatagc accatttgca atgttaccgc
     2461 ttccatcgta atgatttctt ggcatgtaag nnnnnnnnnn atctattgca ccatcttcac
     2521 cttctccatc atgttccagc tcttcactgt cagccttacc tgtcagtcct tccagttttg
     2581 catactgtac ttcgtctgcc ggtacgctga aaaccagagt cagggtcata atttttcctt
     2641 cgcctgatat aactacatct gatgcattta cagcaacttc ttcctctcca acctggattt
     2701 tctccggaat actagtttca tcaaatatat ttccttcttc tgctgtcaga gcgattgtta
     2761 aagtataatt ctgtccggct tcaaattttg ttacagaatt tccatcttta tttgtccatt
     2821 ttgaagtagc agcatatccg aatccatctg tctgtgcatc tttcggttca gcgtctttaa
     2881 caggtgctgt taccgtaatc tcaggttttg aaagtacgga atccccacct atctcagccc
     2941 ctttgaatgt ataggtcaga gtcatggttt ttccgctgtc ctttacttct gcggtcactt
     3001 caagatcttc ttcgccaact ttgatggtgg acggtttgct ttcgtctgtg aatttataac
     3061 ctttttctgc tgtcagggta gctgtcaggg tataatcttt tccatcttca aattctgtta
     3121 ctgtgttgcc atcggaatct gcccatgcag ttgcaacggt gtacccttcc ggctcagccg
     3181 cagttacgtc agcaggtgtc tctcctgtta caggtgcaac tgctgtaagt acaggcttac
     3241 ttatgacttt gtcttctgca acaacttctt ctgctttata aatatcaatc gctgcggcat
     3301 taatataggc atctgtctga tcgcctgctg aatgttttac ttcgatannn nnnnnnngaa
     3361 tgtcacattt ttttcatcag aattattctc ccaatctgct gttgccacct gcttccagtc
     3421 tgtgatcgca gaaacgtcat taacatctgc attggagata taaatattgg cagcggtaat
     3481 tcttccgttt gcaacaccat ttcctgatcc ctgacgaggc agataagcaa tctttccgat
     3541 cgtttcagag cctgcaaggt tgatatacag attgtttctg gaaatcagtt cagtcggagt
     3601 agtcatcgtt ccaggacgta ctatttcagt aaatccttca tctgcggcaa ctgttccgcc
     3661 gtttccgccc cagcttgaat ggtatcctgt agcttcattg ttatccacaa gattcttcaa
     3721 tgctgcatcg ccctgggagg aacactctgc ttttgccaaa atattgccac tgtctatttt
     3781 tgtactgtca tccctgtata cataaagttc tttgccggaa ataaaagtat tgttcggatc
     3841 acctgcagtg ttcagtactt ctatcttaat ttctttcaca tcacttattg catctgtaaa
     3901 ttctatcgtt gctttacttt ctgtaaaact caacgtatat ggatcttcct gtactttctt
     3961 gaattctgca ttttcggctg atgtcttata ataaatattg cattttgtaa tcgtaccatt
     4021 attgcctgca ccatttgaaa catattccaa ttttttgaca gttgatgctt cagtcaaagt
     4081 caaggtgata ttgttgttcg aattcnnnnn nnnnnacttc gccgccctgt ttaaccggct
     4141 gattatcacc ttcccatgct gaatgccagt agtcgtctgg gtttccattt aatacagcat
     4201 tcattgtatt tgttccatta gcacaatctg ctgaagctgt cagaccctct gtaagttttg
     4261 tataattata attctttgtt tctgcttctt ccgaagctgc aagagcactc aatgctccaa
     4321 gtccggatac cggtgcactt gtcacaacca tagtacctac cagtaatcct gctaaaacct
     4381 gtttgaattt catacgtttt taccttttcc tttcataaat atttacggca gacatctgta
     4441 tcgctgtctg tttctccctg tagtaactgc tgttactgca ctgtgaaaca tctcatgctg
     4501 ttccactctt attttattcc aagacttctt cgcctcaaaa ctagttatat tataatatac
     4561 cttttattct atttcaagtc tttttgtaat ttatcaccag ttttatcacc aatcttgcgt
     4621 ttctgtttta ttatttgtgc tgctttttta attattttct gttatttcct gcatttttcg
     4681 tgcatatcaa aaccattttt ttgagaattc ttttgctgca caggtatatc aaaagcaggt
     4741 gtatcagctt tatatcgctg atacacctgc ctgtttttta aatcatatac tatatgccgg
     4801 atatcacacc agtttgacgt aaattactca atcggatctg tttcaatgat aaatttcaca
     4861 ttgctgtcca caccggatgc ttttccactg aaagtatcat atgtgcactc atcagagctg
     4921 agtgctttga tgcggtcgat caccgtgagc agatcatctt ctacggtttc tttgagtttc
     4981 ttggtgcctt tttcttcgaa ttcagaagtt ccgtctgcca gttcttttgc tccgtctgcc
     5041 agctggctga taccatctgc tacctgtccg cttgcggtct gaagggtacc ggctcctgtg
     5101 aagagctcat ctacgccgga aagcaaggtt gcagaaccat ctgtcagagt tgcattatta
     5161 gataccaggg tagatgctcc tgctgaaagc tgcgtagaaa gctgagatac accgcttgcc
     5221 agctgtttgg ttcctttctg cagtgtcttg acccctgcat tcagtaccgg tgcgttctgt
     5281 ttcagttctt ttgctccgga tgtaagctga tcgttgtatg agccaagtga atcgaatccg
     5341 ctcagcagac ttgcgattcc ggctcccgcg gtcggaagag atgcagtctg tgttttcaga
     5401 gtctttacat ttccggcaag tgtgtcgatt gcagcatcca gtccctgcat gcctgtattc
     5461 agtactttga cactggttgt cagctgtgtc atcggtgctt ccggaatgct cacaccggaa
     5521 agtgttgcca gttttgtctg catgccatcc agttttgtgc tcatctgtcc agctgcactc
     5581 tgaagggtct caaggctggt cttcatactc tgaagtgttg ccgtcagtga tgccgtgtcg
     5641 atacccggca tgctgatttc gacatccggt gccgttacct gatctaccgc tgtcacgcca
     5701 gtagtatctg cagatacggt ggtatccact ttggatacgc tcggcagatt tgattttgca
     5761 gcggtcagag aatttaccag atctgtattg ccggctgcct gtgctgctgc aatcgctgta
     5821 tcaagagcgg ctcttgcact ctcaatctga ccattcgccg tgtcaatctg accatttgcc
     5881 gctgccacct ggctgtttgc actttcaact gcagaattca cagaggattt tgctgcatcg
     5941 agtttgctgt tgcggtctgc aatctgtgca ttgacattct gaacggatgt gcccattgct
     6001 gttgcgatcg cagtctgtgc ctgtgtctgt aaagtagtaa gggtagtgcg gattgtctga
     6061 agctgtgctg ccagtgactg tccgtctgcc atcagctgtg ctgccggtgc tttgatggag
     6121 ctgtctactg aacctgaaag ttctttgccc tctgtaatca gttcacttcc tgtctgagca
     6181 agtccgttaa cctgtgatgc cagtccggac agactttcaa gagaactctc aagctgtgcc
     6241 gttccggctg ccagagcaga agaagccgcc ttgagatctt cctgtgtact gccttctccg
     6301 tccattgcgg catacatctg atcgatcgct gtgctcacct gtgaaagacc tgttgtcaac
     6361 ggtttcagtg cttttgcacc gtctgccagt ttcttttcac ctgcgatgta agaagtcaca
     6421 ccgtctgcca gtgtggaggt tccttttgta taggtctgta caccggatac gatgctgttt
     6481 acaccatttt tgtattctgt tacttttccg gtcataacac ccttgcttcc gagatatttc
     6541 tggataccct gattcagctg ttctgcaccc tgtgtataag cgttgattcc gctgtccagc
     6601 tgttttgcac cgccgttcaa ctgatcgata ccatccttta aggtctggat tccgtcatca
     6661 aactctccgt atttctcatt cagagtatct gcaccgtcag acagctctcc ggaaccatca
     6721 accagtttca gtgcagcatc ctcgagatca tccagggcat ctgtcagctc atcggtatcc
     6781 gagatgctgt ccatatccag ctgttttaaa atgtccgtca ttgctacggt gaaggtggag
     6841 tccatctcga aatccgttac atccgctgta atctccagac tctctggaag tgtgatatct
     6901 ttttcctgat ctttggaaag gttcaggctt tcttttaaac ctggcattgc cacaccgatc
     6961 acaatatttc tgcttccatc ggataccact tttccattgt cgatcacaat gttggaaaaa
     7021 tgttcatccg gaaggatcat tccggtcacc ataacaaatg gattataaat attttctgtc
     7081 ttgccatcga tttttgttgt tgtttttgct ttgttttcat aatttacctg aatcttcagc
     7141 tttccactct tgccgaccag atcatttgga tctgtttcta caccatccag aaaatattta
     7201 agctttacag ataccggaag ttctttgctt gtagtaccct gataataaat atcggaaccg
     7261 tctgcctgcc agctcatgga gtcaccgctt ccggtgtaag tttcatcgcc ctttacattc
     7321 tcgatatcct gcagatcgga tttgtctttt atcgtactgt caccattgct gtttttcaac
     7381 cagtttgata cggtaacgtc tgtcgtattt ccggatgcat ccgcattgac ataaacggtt
     7441 tcttcttttg atacatctgc ggcagctgca cttactggca cgcnnnnnnn nnnccattcc
     7501 cactgcaatt cctctataaa caactttttt tgtatttaca ttttttctga ttttttttgc
     7561 catgatctat tacctcctgc tgccgcctct tctgcgacat tttctaagcc agcccctctt
     7621 ttcacgtgac attatatgct ttgcggcttt tatatttcat tagtcactat tgagatgatg
     7681 tataattttt cgacacattt acggaagtgt acaatttttt tacatcgccg cttcctttgc
     7741 ctatttttct tctttctaag aagtagtata gtagaaaaaa gttaagaata ctatagagtt
     7801 tgtttgtgtt tttttctcat aaatgaaaaa gaaggtgata ttttgaatac acaagacgta
     7861 acacgctccg ttatccgtct ggaactcgca agagcttttc gcggtatgga aagtaatcca
     7921 aaacgaacgc tccgcaaact ggcagatctc ggaaaagtat gtgccagagg atcggtgcaa
     7981 aaagaaatat tcggcatttt tcagcagtgt ctcagatata aagacagtcc ttatttttct
     8041 gttatgcaga atctcgtcag aaatgtatcg gcagaaaatc tgactacttt cggtatgaac
     8101 ctcggttata acagctggac ggacggtgca aaaaaaatcc gtgcaaaaaa ggataatgaa
     8161 ggcatcttaa cttcctggat ccagatactg gatctctcac agagcgaatg gacagttgaa
     8221 aacctcaatg cttttattga tttttggaaa acatatggtg tctgcactta tgctttcctt
     8281 ccatctgcct ccttttcaga gaacacgttt ctgccggagg tatttcaaaa gcagaatgac
     8341 tgtgcattct tatggtttct tccggatctg gattctttct gtatcaatat ggatgattat
     8401 aaagatgtgc agaatgtaat gccaatcttc aaaatggaag ctttaatgga atctgaaatc
     8461 catcagatat taagtgccca caaaaaactt tatggcgttt atcagctata taatgaacag
     8521 aatatccgnn nnnnnnnnct gttgattttt attctgatat cacacggatc aatgagatta
     8581 tttcaggtga tccgctcccg attcatatca cagaacggat gaatcggctg atttcttctg
     8641 tttaacttcc agaatctttt tcgcccccag ggaaattgtc tcatccagct gtcggacaaa
     8701 ttctctgggc tgcataatat tctcatccat tatccagcgg ataatcgcca tcaaaaaagc
     8761 atctccaaaa aaccgcagaa aaaaagcttc ttcttttccg gcacccatga tctgatccgc
     8821 aagatacttc agatatggat ccatcacttc tgaaaaatat tcctggaaag aattctgtcc
     8881 ttccatcttc aatgccacac ggtaaaaatc tctttcctga tatagataat cgcagatatc
     8941 ttccagaagc tgcaatgcat ctttatattc ctccagatgg atattgctca caaattctgt
     9001 ataaaagatc cagttgatca gatcgtactt gtctttgaaa tgataataaa aactgttgcg
     9061 attcataccg catgctccgc atatatcgga aatgctgatc ttggaaagct ttttttcttt
     9121 catcagagct ttcatcgccg ctgccatcgc attccttgtg atattggaat ctgccatttt
     9181 ctcacctcat aaacacactt ttcctgtatc ttataccatc cttccaaaag gtgcaagcaa
     9241 cttttttcca ttttattgca tttttttcac ttttcttcta tactgaatga aacaaaacag
     9301 tatctgtaat atcgggccca ggtcgaagga cttcctgcca aaatcgt
//
LOCUS       DAWDWH010000067         9229 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3725_length_9229_cov_12.480161, whole genome shotgun sequence.
ACCESSION   DAWDWH010000067 DAWDWH010000000
VERSION     DAWDWH010000067.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9229)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9229
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3725_length_9229_cov_12.480161"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1755..1764
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2875..2884
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2149 a         2277 c         1964 g         2819 t
ORIGIN      
        1 gtcctctgct ccagcatcat cagtgcctga tttaagctgg taagctcaaa ctggatcgac
       61 tgatccccgg tagcctccat atctgcctga cgctgtgcga tataggcttc cagctcctga
      121 cagccttgtt ctccggcaag aatgtatttc accagctcgt ggtaataatt tacatttgcc
      181 tcaaacatcg tctctaattt gcggttggac tgcttgatct ctgtctcata ctgtttgagc
      241 tgaacataga tctgatctac ttcttcgccc attgtatgat atttggcaag aatcttgtcc
      301 atctgtttgc gaagcccgcc gaacagtttg tcaaacagac ctttgttttc ttttaattca
      361 tccggatcaa acttctccat gatctttgca agcgtgttca gcatctgact ggactcatcc
      421 agctgtttca tatccatgct gtttaacacc acatccgatg ctttggagat ctcttctgca
      481 acctctgccc caaacgatac gatcgtctcc agattgttga cctcgatcgt gctgacgata
      541 tcgtccactt ctttggaacc aaccaactct gcactcatct gctttctgtc cacaacgata
      601 tcataagact tcacctgttc cagttccgcc tgtgcggccg gagctgtctg cgttggagca
      661 tctggtctgt taaaattaag tcccatcttt tactccctcc tgaatagtat gtcccgccag
      721 gatttctggc gtgcctttgg taatggaacc ctcgtcagat ccgggacatt taaatcatat
      781 aaatactccc cgatctgatg ctcttccatc tgttttctgt tgtaaaagtt ctctatattc
      841 tgatatccgg tcggtatgaa aaacagaatg tcaaaaccgg ccagattcaa aaacgcggta
      901 aggatcgcat cctccagtga gattactttc tcaccgggat tgatatagat cagtttcgga
      961 tttttctttg taaaatcaaa tttctgaagc atccgcagga tttctttcgg caggttcaat
     1021 atcgtcgaaa ggatcgtgta ctccgtgccg ttttcaaagg tgccgcggat cagcttctgc
     1081 tcgatcagga tctgcagctt gtccagaatg tgctcctgga tctcttctct taaaaagcca
     1141 taggcatacg ccggatggtt tttgatcttt gccttctgca gtctgccgtt tttgtaaaat
     1201 tcagtcacat aaggcttgat cggattggct gcaagcggct gaatataagg aaagctttta
     1261 atcaccatgg tatcttctgt gatcagctcc cggatcgatg accagtactc ggatactttt
     1321 ccgtctttta cgccggaaac ttttgcaaaa atgaccggga tattgacagt gctttccgtc
     1381 gtactgaaat tcggccggta tttcacttct tcgttccata aaagccggat ctcctcgtac
     1441 atcgtctgga gattgatgat atctgccctc tgatactgct ggttgcggta cagtccacta
     1501 tcctgataca tcagagtatc cagttcccgc tccgcatgat aggcagccgt ccccatccgg
     1561 acttcggtat tttcctgcgg aaaacgctgt aaatgcagcg tctccgtaaa attcatctga
     1621 tagagcagct gatcttccag tacaaacgca gcactgcgat ccggatcgag gatcagtaca
     1681 tccaccggaa gtctgcccag cattttcaga aacaacgctt caaaacgatt tttgcatccg
     1741 cccatataaa aaaannnnnn nnnnaaactt ttgcgatctc ctccggagtc ggcggctcga
     1801 tctgcccgtt cacgatcaca atgtttcgct tccgggcttt taattcctgg taaagctggt
     1861 aaagctcgtt agtataagag attttatctt ctacaccatt tatctggcaa taacaattat
     1921 aataaaattc aggatcactg ccccgcacgg ttggcggctt tgcaatatcc agaagaggct
     1981 ttccctcaat ccatgcattg gtgcagtttc tgacatccgg aagtctgccg taaaggcggc
     2041 ttcgctccat ctcctgttgc tgctgcatgc gaagattctt cagactgaaa tcccccggaa
     2101 atgcctgcat atccggcaag gtcagcggca tagaatatac attcgccgca tccagtctct
     2161 gataattctg atctccgtgg tactgcaaaa gtacaatatc gcagcccgca tggcacaata
     2221 tagacaaaag catcagctca taatggctga tgtctccctc gtacagaatc ttcgggacgg
     2281 aattttcacc gagcagattg acgatccgct cgaacttata atacagccag cacatgaact
     2341 tgatgtaggc atttttcagc ataccctctg tcttgcctgc cctttgcata gaagcaaaaa
     2401 aatcatacat ggacatcgcc atatttttcc gctggtacgg attcatccgc ggaagccatt
     2461 tctgtaaacg actttcgata aaaccaggag ccatctgaaa atccatgccc atcatctctt
     2521 cataataaga aagattctgc ccggcaggat tgggaatcct gccttcgatc acaacgccgc
     2581 gaagcctcgc ctcctcataa tatttccgga taaaggccgc gacctgcggt gtataaccgc
     2641 tgatgcggta aaagtatact tcttctcttg tcgttctgac cgaaagctct ttaaaataat
     2701 cgtcaagtat gcttaatttc tgttgtctga acataggtga aatgcgaatg ccgccctctc
     2761 tttttctgta aaattacata taaatcacta tatcattttt tgcgtttctt ttcaagcatt
     2821 ttaagaaacg accaggcaat cgtttctctt tgcatactgt cgccgtacag atacnnnnnn
     2881 nnnnaaattg gatccagacg tgacaatttc tgctttttag cgtatactca aagtagataa
     2941 aagaaaagga ggtcatttta tgcttgttct tccaaaaaag acttcgcacc cccttcacac
     3001 agattcgatc cttgagatca accgtgtcac ctccagagct gaggactata caggcataca
     3061 caacataaat cgcgtgacgc aaagtgaatc tctctcctct gcagaagccg cgccattcac
     3121 tgtatccagg gcatcggaaa ctcctatgtc atcccgtgtt tcgaatccat caccgtctta
     3181 tcggagatcc gcacagcaga atacattcac gtcatctgct ccttccggca tctcacacca
     3241 gatcatgcct gctctgataa atcaaacgag gaaaggacag aaaacactac ttggagatgc
     3301 aatttctctt ggaaagatca aggcctgcct tggctggaat gtcaaaaatc cggcttgtga
     3361 tgtggatgtt tccgcatttc ttcttggccg tgacggaaaa gtttcaggcg atgactggtt
     3421 tgtattttac gggcaaaaag aaagtccgga ttacagtgtg attttttctg atcgggaaaa
     3481 cacggaccgc gagtccgttg cgatcgatct ttccagactg aatccattga tcacccggat
     3541 cgtctttgtg ttgacgatca atgatgcctt ttccaaacgc ctgaatttcg gcatgatcga
     3601 ggatgcctat gtgcggattt tggatgccgc ttctgaccgg gagctggtca gctttgtgat
     3661 ggatgaatat tattccaatg tcatctccat gatgatcgga gaagtctatt tacataacgg
     3721 aaactggaaa ttcaatgcca tcggaaacgg ggtcgccaaa gacctggaag gcttatgcgg
     3781 tctgtatggt gtacaggtta aacagacttg aacaccgttt ctttttaagg aggaaatcgt
     3841 tttatgttaa attttgaagt gatcaatgaa ctgagtctga aagttacctg caccggaaat
     3901 gatgtactgt tcacaaaggc cggtgctttt atcgccggcg aaagccaggg cggaaaaaat
     3961 tatcgtttcg aaaaactgct gctcggtcca cagagcaacc tgatgcaggc agccctcggt
     4021 tccctgacaa gacgcgtcac ccgtgaaaat ctgcctctga tgaaagtcac tatgaatggc
     4081 gattctgtca cttattatgc aaatcatgga cagcatgtag ttgtgtataa acttgcccag
     4141 ggtgaaatcc tttcggtaga gtccgagaat attctcgctt ttacacagga ctgtgattac
     4201 agcgtccgct ttatcggctg cggtatcttt tcccagaaag gtctggcaac ctcaatgctg
     4261 accggagccg gtccgaatgc ctatgtcgct gtgctctcgg acggcaaccc aatcgccatg
     4321 gacaacaccg acagccatgc tacaatttct gtagacccgg atgccgtgat ctgctggatg
     4381 agccgccgcg gatatggcga tccgcagatc cgcagtgatc tttcctggaa aaacctgatc
     4441 gggcaggctt ccggagaatc ctatgcctat gaatggtccg gcaacgaacc ggtctgcgta
     4501 cttttgcagc catgtgaacg ctccggcggt atccgcgtcg gcatcgatta aatgcaattt
     4561 tattattcaa gattcacgta aaagaccgca gggcatttcg ctctgcggtc tcttttatat
     4621 tatcagcaaa tgctgatctt ctcattattc ttctgtttta tttgcaccat acagtgtgat
     4681 cagtttgttc aggtattcat aacgagcttt tgcttctgct tcattcttag cgaacagttt
     4741 cttagccttt tcagggttag aacgcatcag ggagttgtaa cgaacttcgc cgttcaggaa
     4801 ttcctgatat tctcctgtcg gagccttgct atccagagtg aatgcatctt ttccttctgc
     4861 ttttgcagcc ggattgtaac ggaagcaatg ccagtaacca gcttttactg ccagttcttc
     4921 ttctgtctga gcttttgcca tacctttctt gataccatgg ttgatacatg gagcgtatgc
     4981 gatgatcagg gatggtcccg gatatgcttc tgcttctgcg attgctttta ccgtctggtt
     5041 gaagtcagca cccatggaga tctgtgctac atatacatag ccatagctca ttgcaatgct
     5101 tgccatatct tttttcttca cttctttacc gcctgctgcg aactgtgcta ctgcaccggt
     5161 cggagtagat ttggaagcct gtccacctgt attggagtaa acttcagtgt cgaataccat
     5221 gatgttgata tctttgccgc ttgccagtac atggtcaacg ccgccgaatc cgatatcata
     5281 agcccatccg tcacctccga agatccactg ggatttctta gccaggaaat ctttgttctt
     5341 gacgatgtct ttgcatgtat cacagtcgat gccttccaga gctgctacca gtttgtctgt
     5401 agctgttccg tttgtagcac ctacgctgta agtatccagc cattcctggc aggctgcttt
     5461 cacttcatca gaagcttttt cgtttgcagc aacagcttct acttttgctt tcagtccttc
     5521 acggattgct ttctgagcta acagcatacc ataaccgaac tctgcgttat cttcgaacag
     5581 tgagttgctc catgcaggac cctgtccttt ttcgtttacg gtgtacggtg tggacggtga
     5641 agagttaccc cagatagagg agcatcctgt tgcgtttgca atgtacattc tgtcaccaaa
     5701 cagctgtgta accagttttg catacggtgt ctcaccacaa cctgcacagg cacctgagaa
     5761 ctcaagcagc ggctgtttga actgagaacc ttttaccgta ttttctttga atttttctac
     5821 cacatctgct tttgcaggca gttttgttgc atagtcgaag tatttctgtt cgccggcatt
     5881 tgcttccatg ttttccatag ccagtgcttt tgcacctttc ttgcccgggc agacgtttgc
     5941 acaggaacca catccggtac agtcgtaagc ggatacagtc attgtgaatt tatattcttt
     6001 cataccggtc atcggcagag tcttcagtcc ttccggagct gcagcaacct cttcgtcagt
     6061 cagtgctacc ggacggataa cagcgtgcgg acaaacgtat gcacagcggt tacactggat
     6121 acagttttcc ggattccata caggaacgtt tactgcgata ccacgttttt cgaatgcagc
     6181 tgcaccggat ggtgtcgtac cgtccacata atctttgaat gcagatacag gcagtgtatt
     6241 accttcctga gcgtttactt tgttctggat gttgtttacg aaatctacta cgtcctgacg
     6301 gtcgcctgtt gcaactttgg tatccagacc ttcgtcctgt gcgtctttcc agctttccgg
     6361 aacgtttact tcaacaatct gttttgcacc ttcatcgata gctgcccagt tcttctgaac
     6421 gacatcatca cctttacgtc cgtaagttgc ttttgctgct gctttcatca gctcgatcgc
     6481 tctttcttcc ggaatgatgt ttgccagttt aaagaatgca gactgcagga tggtattgat
     6541 acgagtcgga cccatgcctg tctcaatacc gatcttaaca ccatcaatga tgtagaattt
     6601 gatgttgtgc tttgcgatgt atgcttttac ctgacccggc agatgttttt ccagaccttc
     6661 catatcccat gggcagttca gcaggaatgt accgccgtca accagttcct gaaccatgtt
     6721 gtatttacga acataggaag gattgtggca tgctacaaag tttgcttttt tgatcaggta
     6781 ggtagatttg atcggtttct taccgaaacg caggtgagac atggtaacac cgccggattt
     6841 ctttgaatca tagtcaaagt aagcctgagc gtacatatct gtgttgtcac cgatgatctt
     6901 gatggagttc ttgtttgctc caacggtacc gtctgctccc agtccccaga acttgcagtt
     6961 gatggttcct tccggagttg tgatcagctg ctcgcccagc ggcagagaca gattggtaac
     7021 gtcatcttcg ataccgatcg tgaatacttt cttttctgtg ttgttgtata cagcaacgat
     7081 ctgagtaggt gttgtatctt tggaaccaag tccgtaacgt cctgaatata caggaactgc
     7141 atcgaatttg gaacctttca gagctgctac tacatccagg tacagcggtt cgcccatagc
     7201 acccggctct tttgttctgt caagaactgt gatctgtttt acagattccg gaattgcatc
     7261 aatcagtgct tctgcacaga acggtctgta cagacgaact tttacgacac cgactttttc
     7321 accggcagcc atcaggtaat cgatggtctc ttcgatggta tcatttacag atcccatagc
     7381 tacgattact ttttctgcat cagcagcacc gtagtagttg aacagtttgt agtttgtacc
     7441 gatctttgca tttactttgt ccatgtattc ctgaacgata gccggcattg catcgtagta
     7501 agggttgcaa gcctctcttg cctggaaaaa gatgtccggg ttctgtgcag aacctctctg
     7561 gcaaggatgg ttcgggttta gtgcatgttt acggaatgca tcgattgcat ccatatctgc
     7621 catatccttc agatcttcgt aatcccatgt ttcaatcttc tggatctcgt gagatgtacg
     7681 gaaaccatcg aagaagttga tgaacgggat acgtcctttg attgctgcca gatgagcaac
     7741 cggagtcagg tccataactt cctgtacact ggattcgcag agcattgcac atccagtctg
     7801 acgacaggca tagatatcgg aatgatcacc gaagattgac agtgcgtggc ttgccagtgc
     7861 acgtgcagaa acgttgaata cgcccggcag ctgctcgcct gcgatcttgt acaggttcgg
     7921 gatcatcaga agaagaccct gagaagctgt gtaagtagtt gtcagagcac ctgctgccag
     7981 agatccgtgt acagtacctg ctgcacctgc ctcagactgc atctctgtta cctgaacttc
     8041 ctgtccgaaa atattttttc ttccctgagt tgcccattca tctgttgcct ccgccataac
     8101 agatgaaggt gtgataggat agatagctgc aacatctgta tatgcataag aagcatgagc
     8161 tgctgcatgg ttaccatcca tggtcttcat ttttctagcc attttattat acctccttaa
     8221 aataatgctg gataaaaaga ttccttttgc ggaattcaat tagtatcctt attgaaaaaa
     8281 aattactaat ctgttgttac ccatatattt ttactatagc acaacaaaaa gaaaatgtcc
     8341 atttccgaaa cgacggaaac agactctttt cttgtatttt ttgctgtaca ttccagctct
     8401 tttttcagtt ttattatact ctttttacaa ttttcagtcc aatttcatca atacgtttac
     8461 ttgactgtaa gtttcagtct tgctttcttt ccattgtaaa gtttcacagt gatcactgta
     8521 cttcctttcc ggatggcttt cacgtttccc tttgcatcca ccgtggcaat ttttttcctg
     8581 ctgctgttgt atcggatgcc gaatgcggca tttccggacg gaagtttcac cctgagccgg
     8641 agggtttttc cttttttcag tgtcctgttt tttgcaccga tcgtaacctt ctttggtgcc
     8701 tgtctgaccg tcactttgat gcggcaggtc ttctggtttg cagttgtgac cgtgatcacg
     8761 gcatttcctt ttttctttgc ccggatcaca ccgccctgtg tgacacttgc cactttgttg
     8821 ttgctgcttt tgtaagtcac acgttgatct gcggcatcag caggtgtgat caccggattt
     8881 aaacgaacac tttctctgac tccgatcacg attttggcac cgttcttttc ctgaccggaa
     8941 aaagcagaac ggctgacaag agaaatactc tgcacttcct ttttctccgt gctttctgta
     9001 tgatttccgg ccggatcttt cttttcggtt tcttttccgg tattcggatt cggtgcttcc
     9061 gtagacggct gctgcgtttc tgtttctctt tctgtttcgg attcgctctc cgtttcggtt
     9121 tccgaacttc tgtcctctgt aagttttact tccttttcca gtgttttgct gcctgatgca
     9181 tcttctgccg ttaccactgc agtataagta cctgctttca ctttattgc
//
LOCUS       DAWDWH010000068         9203 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3737_length_9203_cov_11.742020, whole genome shotgun sequence.
ACCESSION   DAWDWH010000068 DAWDWH010000000
VERSION     DAWDWH010000068.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9203)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9203)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9203
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3737_length_9203_cov_11.742020"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2013 a         2415 c         1833 g         2942 t
ORIGIN      
        1 ttctgctttt acatatacct gatccagcag acagatttct ttcagctctt tgttgatacg
       61 gcctgcgatc atgccctgga taaccttttc cggtttctgg gattctttcg gatcgttcat
      121 gatctgagcc agcaggattt ctttttcatg agcaatgtat tctgcactta cttcgctgtc
      181 gtttgtgtac agcggtttca gagctgcaac ctgcattgct acgtttttag ccatttcttt
      241 tactgcatcg tttacaacgt ctgtctcaac gtcaaccaga acaccgatct taccgcccat
      301 gtgtgtataa gaagctacga aaccgttttc ttctgtaacc tgtgtaaaac gacggatgtt
      361 catgttctcg ccgatggttg cgatctgatg agccagttct tcttttacag tagcggactc
      421 gctgaatgtc cactcttctt ccataaatgc atcgatatct gctgcttttg tgttcagagc
      481 cagttctgca acctgtgcaa catatccctg gaacttttca ttctttgcaa caaagtctgt
      541 ctcagcattt acttctactg caacagcttt tttgccgtct tcggaaactt tcagcattac
      601 gataccttct gctgcaattc ttcctgcttt tttctgagcg gttgccagtc ctttttctct
      661 caggaactca acagccttgt ccatatcacc gtctgtagca gtcagggctt ttttacagtc
      721 catcatacct gctccggtca tctctcttaa ctcttttacc agttttgctg taactgccat
      781 tttattttcc tccggataat aagtggtata tttgctttgt atctattgat tattcagctg
      841 cttcttctgt ttctgcagct tcttcagcta ctgcttcttc tgcaccctga tttgcttcga
      901 taacagcgtc tgccatcttg gaaacgatca gttttacggc acggatagca tcatcattac
      961 ctgggattac ataatccagt tcttccggat cacagtttgt atctgcaata ccgatcagag
     1021 tgatacccag tgtatgagct tcctgaacac agattctttc ttttttcgga tctactacga
     1081 agatagcatc cgggattttc ttcatgtctt tgataccgcc aaggtttttc tgcagtttat
     1141 ccatctcttt tttcagagcg ataacttctt ttttcggcag tacatcaaaa gtaccgtctt
     1201 cctgcatagc ctcgatctct ttcagacgtt tgattctgct ctggatggtt ttgaagtttg
     1261 tcagcatacc accaagccat ctttcgttta catagtacat tccgcatctc tcagcttctg
     1321 tcttaacagc atcctgagcc tgtttctttg tgccaacgaa aagaacggta ccgccgtcag
     1381 ctacgatatc ttctactgct ttgtaagctg tgtctaccat acctactgtt ttctgcaggt
     1441 cgatgatgta gataccattt ctctctgtgt agatgtacgg agccatttta gggttccatc
     1501 ttcttgtctg atgtccgaaa tgaacacctg cttccagtaa ctgtttcatt gaaattacac
     1561 tcatgtcttt tctccttttg gtttttcttc cgtgtgcttt tgcttcctaa tcaactgcca
     1621 tgcagcacca tcttcagaat ccacacacgt gattattttt tgcctctggt agtgtatcat
     1681 aaaaattttc caagtgcaag ccttttttct gatttcttcg tgcttattcg tgacgcagag
     1741 catcgatcgg atcgaggttt gcggcctgta ccgacgggag cagtccgaac acgattccaa
     1801 tcaccatgga aaacagcaca gagatcagaa ttgccggaat actgatcgcc accggtgtcc
     1861 cggaaacttt atggatgacc tgtgccagga tatttcctgc gatcactccg ataattccgc
     1921 caagacttgt cagcacaacc gcctctgtca gaaactgcgt caggatgctg cgtttcttgg
     1981 ctccgatcgc ctttttcagt ccgatctcgc tcgttcgttc ggttaccgat accagcatga
     2041 tgttcataac gccgatgccg ccgaccagaa gagaaatact tgcgatccaa atcagctgcg
     2101 tgttcatcgt ggcactggaa ctctggatct gtgctgcaag ttccagcaca tccgttgctt
     2161 tgtattttac atcgtcttct tttacattca tgtagccatt caggatatct gcacttttct
     2221 gtccgacttt tgtcatcgtc tcggtggatg atgcacgtac cacaacattc tgcggctcgt
     2281 catactgata cggaatgcac cagcatgcgg aaggaatgta gaccacgcca agactttcgt
     2341 ttcctttata gttattatat tcttcaaagc tattgatcgt cggctcaaat tcttccagtt
     2401 tctgtgcaat accgatgacc gtaaacggct ctttgttgat ctcgatcgtt tttccgagcg
     2461 gactttcctc ctgaaacagg ctcgtcgctg ccgactgatc caggatcacg actttatgga
     2521 attctttgta gtcctcttcc cggaaggtac ggccggtacg cactacataa ccggttgtac
     2581 tgaaataatt gttgtctacg ccaagtacct gtgctccctg aagagagtta ttattataat
     2641 aaatactatc ggcataactt ctgctgtagt aggcggcggc actctccaca ccgtccacgt
     2701 ctttgatctc ctgcagaata tcatccgtta tggtcggcac gccgctcggt gcctgttcgt
     2761 aatccgacac ctggattttt tcatctgcac ggtaaagctc tatatctacc gtattatttc
     2821 cgccgccgat cagattctgc ttgatctgtt cgttcgttcc ctgaatcgta gaaacaatgg
     2881 aaataattga tgcaataccg ataataatac cgagcatcgt cagcatagaa cgcagtttat
     2941 gcgaccatac accctgaaat gccagtctta tattttctaa cacattctac ctcctgtttt
     3001 caactgcacg gcaagtactg aaaaacgcat gcccgatcag tataactgat ctgcgaatcc
     3061 gcttccttcc agttctttta ccggtgcacc ttcttttact gcttttccgt atggaaaggc
     3121 gatccagtct gtctcactga ctccttcttt tacttcaata taggtggaat ataatgtctg
     3181 tcctgtacgg acatactgtt tcttcagttt tttatttttt ccttctatat atacatagct
     3241 ctgcccgttt tccatgcgga tcagtgcttt cggcagataa acggaagatc cgtttccgcc
     3301 gtttccttcg atctggatat ccacgtattc atgattgatc aaatcatcag aatcctcaat
     3361 ataggcagta aaaggatagc tggaagcatt cggattgctg tctccgccat aataattgcc
     3421 atcagcgtta gacggatatt tggaaatctc tgtgatctct gctgtgatcg gtgtaccact
     3481 ctcctgtgcc gttccggtaa ttgtggaacc gacaacgacg tttccgagat ccatctcgct
     3541 gatgctgccc ttcacataca ttcctttgtt gcttgtcact gtcagaaaag cttctccgtc
     3601 aacctgtccg actttcggat cgccgacggt tttcaccaca ccgttgattg ttgccttcac
     3661 cgtagcctca tccagttttc tctgtgcctg gctgacactg acttccgcat cacggatatc
     3721 cagctcaagt tccttgattt ctttttcttt ttcggcgatc gcctgtttga gttccgatac
     3781 ggtataaatg ccatcacccg gttcaaacgg accatcaccc ggatcgtctg gttcatccgg
     3841 atcgtccggt tcatccggat catcgttctg cggatcctgg atatccggca cttttttggt
     3901 aatgccctgg ctggtaaaga tccaggtcac atccggagca taggctttgc ctgcatcgat
     3961 cgttccgttg atcgtcacgg atttgatata tccgcccgcc agagaatctc cctctctgat
     4021 ctgcaaaact gcatagcagc cttttccgtc tttattcaca ccgccttctt ttctggaact
     4081 gccatcggta ttgaatccaa ggattttgtt catgaaagat gccgaaacct ctgctccttc
     4141 tttgcagaga tacacatacg gatctttttt cgttccgctt cctttatagt atttcgtctt
     4201 ataatccagt ttctggtacg cctttgtctt tgcaagcgga gaatcttccg tctcttcttc
     4261 tgtttctggc tcggattccg tttctgccgg atatgccggc tccgtggaag gcatcgtgct
     4321 ttccggcgtc tggctttcag tctgctgcgt tgtctccccg ccggccgttc cggtgctgtc
     4381 actgccatcg ctgtttccgt caatgacctg cacatccgca cctactgccg tggaaaccag
     4441 tgaaacatta tgtttcacaa tcgttgccct ggcttcctca tatccatctg ctccactgtc
     4501 atccagatca tctgaactgt catccagaag agaggcaccg gatgagctgc cttcactgtc
     4561 cggcactggt gtgatcttct ttaatttttc caaatcattt tttgctccct gcagttccaa
     4621 ctcctgaatc tgtttgttga tctgttcttc ttccagattc agctgcagaa gggtcttgtc
     4681 atattccaga agggtatcgc caatcttgac tttgtcaccc tttttcacat aaactttctc
     4741 taccacgctg tcgctttcca gatagacatc ctgagaagca tttgccgtaa cataaccgct
     4801 gcttgaagtg ccttcatccc accagccgct gttcaggtaa gatacctgcg tgacatccac
     4861 ggcactgccg ccgcctctta ctgctcttgc gatccccgtg attgtgatcg cacacaccag
     4921 taccgctgcc gttatgatca tcacattctt tttcttcaaa gagtttcctc ctttcggtca
     4981 cgttctgaaa tctcaccgtc aaaaatgtca acgatccgct ttgcacactg tgccacatgg
     5041 ctgtcgtgcg tgatcatgac aacggtaact ccttcgtcat ttaacttctg gaaaagctcc
     5101 atgacctgat gactggactt ggaatccagt gcaccggtcg gctcatccgc cagaaggatt
     5161 tccggattgt tgacgatcgc cctggcgatt gctacacgct gtttctgacc tccggaaagc
     5221 tgattcggga taaagttcat acggtcagaa agtccgacct tgtcaagtgc cgccgccgca
     5281 cgtttcatac gctctgcttt cggtacgccc gcatagatca gcggcagtgc aacattatcc
     5341 agtgcagtct gtcttggcag aagctgaaac gtctgaaata caaaaccgat cgtattgagc
     5401 cttagatccg ccagatcatt gtctttcata ccgcccacca tatgaccgtc caggatgaat
     5461 tctccctttg tcgcacggtc caggcagccc acgatattca taagcgtgga ctttccggaa
     5521 ccggacggac ccatgatcgc cagatattct cctttctcta cctggagatt gatatccttc
     5581 aagaccggaa caaccatgct gccctgaata tagtctttat atatatgttt caattccaat
     5641 atcactatct tttcctccaa tgccgcatta ttctgcactt gcaggacgat ctccggtttc
     5701 tgctgccccg tccggcatgc tgcctgcact gtcatctgca ctgtcgtccg gataatcttc
     5761 ttcatccggc atattatcat ccgcactgtt tatcatatcg gatgcacttc cggcctcctt
     5821 tggcataaca tcctcgtcag atcctcctat gatctctcca tcatcagatc cgttcatgac
     5881 atccgaatcc acaccaccat ccatacttcc atcagagcca tcatcgctgt ttccatatat
     5941 atcttcgata ttttttgtta ctttttcgcc ctctgtgatg gaatcttccg ggtaagcgat
     6001 ataatcatcc tccgtcagac cgtccgtgat ctgatactga tcgagttttt catcatattc
     6061 gccaagcgtt actttgcgtt tttcgatctt atcgtgtctg ccggctgccc agacgtaagg
     6121 atcctgtccg tcttcctgga tgatataata gcttgtcagc cacaggccct ctctgttttc
     6181 tgtctggcct tcgtcttttt ccacataaac atgctgtccg agcatcaggt cactgtcatc
     6241 ctccagatca atgtaaaacg gatagttgct ggaacttgtc gtatcacttg aagaagaact
     6301 gctcatataa tagccgctgt cgctgttttc ctccggatgc tccagatcga tcgtgcttac
     6361 ggtacctttc caggtctggt tttcgtctac acgggaatgt acgattacgg catcaccttc
     6421 atttaaagaa gtacggttct gttcatttat cgtacctttg atgcggtaat gtccggtcga
     6481 taaaagcgtc atatacgcat tggaatcatc cgaagaagag ccggaatatc cggcactgtc
     6541 atcactggaa ttgttgatgg attttacgac tccgtcgatc tcacttgtca cttctgcttc
     6601 ctgaatgctc tttttaagct gctcgatctc ctgctgcgtg cttttcagct cgtattcgct
     6661 ctttttctgg ttgttctggg cagactggat acgcacagta taatccagct gatcttcgct
     6721 ggacgcattt gctttttctt tctgaagtgc tgcaatattt gatttgtaag tttcgatatc
     6781 catcttaatg cggtcgatct cgatctcttt gctagaaagg ttctcctccg cttctgatgt
     6841 atcataaata aaaagcttct ggcccttttt gacctcatcg ccttctttta cataacattc
     6901 ttttaccttt ttgtcggatg actgtttgat cgtcaccgtt ttctgcggct ccaccacacc
     6961 tgcgaaacgg ttcagctgtc cggtaccgct tccaagtccc atgatcgatg tcacagaatc
     7021 cacatacacc agatctccac tgtctgaaga accggatgaa tgtcccttca gaaaaacaaa
     7081 tccaaagatt gccgcaacaa ggatcacgcc tgccgcactc ccccctatga tcaatttctt
     7141 tttattcata tttacctcct gcctctgatt tataaacaga tatttctctg acccgtacag
     7201 tataacacat tcccttttta ttgaatactt aattttcttt taaagtttga acgttctgta
     7261 ttcacacttc actttgccat caaaaaggct cccgcatatg caggagcctt tcggatattt
     7321 tattcgattt tttagatcag cggatatttc tccgtcagac ctttcaccag agcttttgcc
     7381 cgttttgcag cattttcttc ctctttcagt acgatggcga tcgcttctgc gatcacatcc
     7441 atgtcttcgg tattcatgcc tcttgaggtg acagctgccg ttccaagtcg gacaccgctc
     7501 gtcacaaaag cggatttcgg atcgtttggt atggtatttt tattgcaggt gatgttgacc
     7561 tcatccagca ggttttctgc ttcttttccg gtctttccaa ggttggtcag atccaccagc
     7621 atcagatgat tttccgtctt tccggaaaca atgcggatgc cgcgttttgt aaggccgctg
     7681 caaagtgcct gggcattgtc aataacaccc tgagcatata tcttgaaatc atctgacagt
     7741 gcttctttaa agcagacagc ttttgctgcg atcacatgca tcagcggtcc accctgtatg
     7801 ccagggaaaa tagctttgtt aaagttaaat ttctttgctg tttcttcgct cgagaggatc
     7861 agaccgcctc ttggtccacg cagcgttttg tgtgttgtgg tcgttgtcac atccgcatac
     7921 gggatcgggc ttggatggag tcctgctgcc acaagtcctg cgatatgtgc catatccacc
     7981 atcagatacg caccgacttc atcggcgatc tcgcggaact tcttgaaatc gatgatcctt
     8041 gcataggcac tggcaccggc tacgatcagt ttcggatgac attcgtttgc gatccttctg
     8101 acttcttcgt aatcgatcac gccgtcatcg tttacgccat acggtacaat gttaaaatat
     8161 gcaccggaaa aatttgcagg gcttccgtgt gtcagatgtc cgccgtgtgc aaggttcatg
     8221 cccatcacgg tatctcccgg ctttaacatg gcaaaaaaga ctgccatatt tgcctgtgct
     8281 ccggaatgtg gctgtacatt ggcataggtg catccaaaca gttctttggc acggtcaatg
     8341 gcaagctgct ctacctcatc cacacactga cacccgccat aataacgttt gccaggatat
     8401 ccttctgcat atttattcgt caatgggctg cccatggctg ccatgacagc tttgcttacc
     8461 cagttttcgg aagcgatcag ctcaatgtga ctgttctgac gttcttcttc tttgacgata
     8521 caggatgcga tttccgggtc tacggatttc acttcatcta acgaatacat atctgatcct
     8581 ctctttctga agttttattc atttgcaagt ttgtttaaat atatataact tttgcaaaaa
     8641 tgtcaagatg tttgacaata tctttgatat cggataaaat aaatttaaat ttgctataag
     8701 gaggacgcta tgaaccggaa atatttttct gcctctctgc tcctgatcct tttgcttctg
     8761 gtcatgcctt tttcgctcgg cggatgttcc aaggactctc cgaaacgtgc tgtcaaaaag
     8821 gaactgaacc tgatcaaaga gctggatgag agcacgatca aggcttttgt ttcctatgag
     8881 gatatgatga gttcccattc ttccagcagc gatgtcggaa tcgagacaac tgaagcggta
     8941 aaacggttct ttcagaattt cgattataag ataaagagtt cttccgtgga ggataagaaa
     9001 gcgacggtgc acgtagagat caccaatctg gatacgcgtg ctctcgcaaa ggatatctgt
     9061 ctggctctcg tcaagaaagg tacgggcagc ggctcatcgg aaaatatgac gctgaactct
     9121 tattttgcac tgcttggcga tgttttatcc gaaaacacct atgatttggt gaccaccgaa
     9181 gcggatatta agcttctgaa aac
//
LOCUS       DAWDWH010000069         9188 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3741_length_9188_cov_14.721231, whole genome shotgun sequence.
ACCESSION   DAWDWH010000069 DAWDWH010000000
VERSION     DAWDWH010000069.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9188)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3741_length_9188_cov_14.721231"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6584..6593
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2226 a         2300 c         1799 g         2853 t
ORIGIN      
        1 tcgctctgac ggtagcaagg cttccgtcgt gaggtttgcg atgctgccgc cccaaggagc
       61 cgctgtctga gcctcgaaga ggcgagttta ggcgaagggc ggctaacagg cgagcgaacc
      121 gaacaggaaa cgcagcgtgt caggagattg ttttcgaatc atcatggcgt tatatatttt
      181 ataaaatcat ttctatggcg ttcatttgca catttacaca aacctccgcc acaacttttt
      241 attgttcttt ctttggcatc tcttttttat actactgaac cgaaatttcc acatcattta
      301 caacaagcgt ctccacctgg ctccagtcca ccatgcggtt aaacacgatc ttgatggtat
      361 ctccgttttc catttcgccg cagacatagt ttgtgttgtc cacattttca tccagaacgg
      421 tgtattcgct tccgtctttg tatttcagca ccagtttttt caggtaatat ggatctacaa
      481 aggtctgtcc gtcttctttc tggatctcat cgtccagtcc aaatccattt gccagattca
      541 gggaaagtga gattgctgaa taagcaaact gttctttgtt gttttctgaa ttcactgcac
      601 tggtcagcgg catttttccg ataccaagtg caatgtcttc tttctgcgta gccatatcat
      661 tatctttcag ttctgaaact ggttttccat actgctccag atgaaatttc agatcagtat
      721 tcttagcaga gcagacatag ctggaataaa gataataaca ggtgtcgctg ctctttttgc
      781 tgtcgatcgt cattttgtct gctacaaggc tctcatctgc accttccaga tagaaataga
      841 agtcacgatc ctgtgtaaaa taagcacctt ttgcctcatt tgttccttca tctgcatgaa
      901 tggctttgat tccgtttgga ttttccagtt tcagagacag aacacctgca tttccatccg
      961 aaatattatt gataatcgtc agggtgtaat ctccgatttt tcttgtgata tttaaatctt
     1021 ctacgtaagg ctccaccagt ttttctgcct gtgcctcatc gactgccaca tattctcttg
     1081 atggcatcgt cacagtcgtg ccgccttttc cattatctac gaccgtatca taagcttctt
     1141 ttgtcgtacg gctctgctgt ccgaacacat tgccaagcag atcggtatgt gcactgatat
     1201 acgcagctcc tgcggttgtg ccggagatca gaagcacggc aagtgctgca gcggctgcca
     1261 tgctggtatg ctttcttccc cgcttttttg atgtttttgt atctcttctg atctgtgcat
     1321 aagtttcctg catccgcttt tctgtttctt ttgatatcgt aacgtctttt tttaatgtat
     1381 cctgtaattc tttttctgta tataacatat actcccggcc tccttataag tattttcaaa
     1441 gtattaactg acttttcaga ctttccctgc cccttctaag tctgctcttc accgtgttct
     1501 cactgagatc cagaatctct gcgatctctc ttgttcgaaa tccctgtcca taatacagaa
     1561 gaaatatatg tttatatttc tccggcagac aggaaagcat ctcataaaat tctgcatcgg
     1621 tctctgcctg cggctcttcg ccctgcatgg aaaaatcttc cgcggcaatg cagcgtttcc
     1681 gttctcttaa cacattgttg cagttgttga tcagtattct ggtcagccac gtcttaaaat
     1741 aagctgcctt tttcagctct cctaaatgtt cccacgcaga aagtgtcgtc tcctgcatca
     1801 catctgccac atcttcttcg tttttcagat aagcttttgc gactttgtag agatccatct
     1861 tgtatttttc aatcagagcg acaaaggcat cggcatctcc cctctgtgcc tgctttacca
     1921 gaaacattgc tgttccccct ttcgttttgc atctttcact tattagacga ggctatgtgc
     1981 catttgggtg catatacaga aaaagttttt tactcgctta cgattttata gtattctctg
     2041 gcggtgatca gatacacgat cacataaaat acggcaaatg ccaggaaggt cagtaccgca
     2101 cagatcacca gcagacctac atcctgaagt ccgaaaccgc caaccaggat ttcttttacc
     2161 agatggaagg caaaacagct atggatgccg gctgctgcaa gcggcagaaa gaataccatc
     2221 agtacctggc tgcggatgga tgcgttgatc tcccctcgct ccataccgat ctttttgagg
     2281 attgcaaagc gttctttgtc ttcatagcct tcggatatct gtttgtaata gatgatcagg
     2341 acagttgcca tgacaaagag cagtcccagg aagattccca ggaaaaacaa tccgccgtac
     2401 aggctgtaga attcggccct gccttccgtc ctggtttcga ttccatagaa aaaggcgtgc
     2461 ggtgcatttt tctgctctcc cagataagca ttcagacttt tttccagttt ctggattttt
     2521 tcttccgaaa gatccacgtc aaagctgtat tcgtatttca cctgagatgc atggtctccg
     2581 taagcttctt tgttgatctt ccagatttcc tgtaactgtg cgaaatcttt tactacgata
     2641 cggtataccg gagttacata aaatttcagt ccgctatctt ccatcgtgct gtcagcagac
     2701 agctgtctca ctttccagtg catatcatta atggaaaatg tttcctgctg tatcggactg
     2761 tcctcaaaaa ataccagtgc ttctctatct tcaagtgtct cgtccttttt tgcttcccgg
     2821 ttataatctt ccagtgtgat tgcctgcacc atgcacagat cactgaaatc ttcggaactt
     2881 ccatttctgc ttgatgtgat ctgtacattt ccatctttct gcagtgctgc cgcctcgatc
     2941 acggtctggc ctgctgcatt ttgagtggca attttgttat ctttcaggta gtcttctgcg
     3001 taagtaccga tctcttttaa ctctttttca aatgttttgc tgtcatccgg cacttctccc
     3061 agactgagag aaagtttaaa ttctttcgga taacgcagat acagagaatg ttcctgtccc
     3121 acataaaggc tggtcgtaga agagatcagc agcaggactc cggttgacat gatgcagatc
     3181 agagacaggc ttgctgcatt ctgcttcatg cggtagatca ttccggaaat tgaaataaaa
     3241 tggtttgttt tataatagta gcgtttgttt ctttgcagca ttttcagaaa tgcaatgctt
     3301 cctgccgtaa acagtgcaaa agttccgacg atgaccagaa gtactgccac aaaaaaagta
     3361 ttcaaccctg tcagaacatt atctgccttc agtgcaagga tatagccgcc tgcaagcgtc
     3421 actactccaa gcaaagccaa aatccatctg gttttcggca ttttttcacc gatctgaccg
     3481 ccttttaata attctgccgg tcgtaccatg tagatcaggc ggacgctgtt aaagaagatc
     3541 agaagaaaga tcacggcaaa aagtccgacg gtttctgcca gcgtaaaagc ggagatttcc
     3601 acgccgatca caacgttcat cttcaggatt ttcagaagtc ccagaaaaac cagcttggaa
     3661 aacaggattc cgcatgcaat tccaagcagc agagagagca ttgccgtata aagcgtctcc
     3721 cacagcatca cacggctgat gtggcgtttc tccataccta aaatataata aagaccaaat
     3781 tcttttttcc tgcgtttcac aaggaaactg tttgtataaa ataaaaacag aactgcaaaa
     3841 atcccgacta ccaccgttcc gaacatcagc acgatgccca tcgttgcccc gcctcgcatt
     3901 ttattcagta cagaatttgt ggaaaggctg gacatgatat aaaacatcat gatcgttgca
     3961 atgcaggtca gaagatacgg aaaacaggtc tgtctgtttt ttttcagatt tgtcagtgct
     4021 attttcgcat aaaagaatcg tttcatgcct cttccctccc tgctgtgatc agggtcagtg
     4081 tatctgaaat cttctgcagc atttccctct gtccggacat tccgcggtag atctgatgat
     4141 atacctcgcc atctttgata aacaaaaccc tggatgcatg gcttgccgcc tgaatgctgt
     4201 gcgttaccat caagatggtc tgtccttccc ggttgatgga ctcaaacaga tccatcacac
     4261 ctgccgccgc tctggaatcc agtgccccgg tcggttcgtc cgccagaatg atcttcggtt
     4321 ctgtgatcag tgctcttgcg attgctgcac gctgtttctg accgccggac acttcatacg
     4381 gatatttctc cagaagcttt tcaatggaca gtctgcgtga aagttccatc attttcggtg
     4441 ccatttcact gtaagtcttt ccggataaga ccagcggaag catgatgttg tctttcagcg
     4501 aaaacgtatc caacaagttg aaatcctgaa aaacaaatcc aagattgttt ctgcggtaca
     4561 tggcaagctg cttctccgga atgctgccca tatccactcc ggaaagcaga acttctccgg
     4621 ctgtcggttt gtcgagtgtt gccagaatgt tcagaagcgt cgtttttcct gagccgctct
     4681 cccccatgat cgccacaaat tctccttctt cgacggaaaa attcacatcc gaaagtgcct
     4741 gcacctgcgt gccgccgaat cttgtggtat atattttttt caagtgtgaa acatttaata
     4801 attccatttc cagtcctcct gccatttttt actgtgtctt ataataccct gcggatcgct
     4861 ttgtatttga tactccacca tccacatgat cgtttcaggt tttcgttcct gacatccgtt
     4921 attgtatgat gccagggtca aaaggtctaa atccacatga gcttgctcat aggtggatga
     4981 aagaccttgg gacccgcccc gatatgagca taaaagtggg atgataatcc cacgatatgc
     5041 gaatactggg gtgctatgtg ccagtggcac atgtttagca ccgaccgaag cggagcgtag
     5101 aaaggattgt gggagtgcaa cgcacggaac aatccgtagg catcatagtt ggggcctttt
     5161 gacacccaca tcattgtata aaaacaggaa ctgcaaatcc atcgaaatgg cttacaattc
     5221 cttttttaac cttacatcta tgtaagtttt cagtaatccc ggactggatc gactgcaaga
     5281 tccagataaa tacatgtccc ttttcccggc tcggattttg ccgaaatacg atgtcccagc
     5341 atttccatgg tctgtctgca cagggccagt ccgatgccgg tggcacggtt cttgatcctt
     5401 ccgttgtacc cggtatatcc ccattcaaag atacgcggca gatcttccgg acggattccc
     5461 atgccggtat cttccacaac cagcgttttt tctttttccg gatccagata aatggaaatt
     5521 tttcctttcc tggtatattt cacggcattg gtaagtacct gctccagcac aaagaccagc
     5581 catttttcat cggtcagcac atctgtctgc agattgccta gctctagggc aagctttgtc
     5641 cggcgaaaga gaacgcctgt ttttttcagg gcttttttga caatactttc caggctgtaa
     5701 tgggcaaata ccagatcttt gtgcccgcct tccaaacgtt gatactggag tgcaaattcc
     5761 acatactgtt cgatccggta gatttctcct tccatggcag cacgatccgg ctcttcttcc
     5821 tctagaagca ggcggagggc agcgatcggc gtcttgatct gatgtgacca ccttatgtaa
     5881 taacgatttg cttcactgat ctgacatctt gcttcctgcc tggcattttc ccactgtctt
     5941 cccaacatct ctgcaagatt ctgatattct ttttcaacag cgttttccgg ctttccaaaa
     6001 tgttccgttt ccagataaac ttctgcttct ttttggatat ttttcagttg tttatatttt
     6061 tttctttgct gcactccatc cagaatgatc catccgccaa aaatcagaag aataaacaca
     6121 ctgctgtaag cccatgcctc cagcggcatt tcatagagat aaaacaagag cgtctccatc
     6181 agaaacaata tcagggcagc catagccttt ttcatctccc ctgctcctcc ctgatctgat
     6241 agccaatccc ttttcgcgtc ttaataaatt cgcaaagtcc gatttcttcc agtttttttc
     6301 gaagtcttgt cacattgacc gtcagcgtat tgtcatcaat aaaatgttct gattcccaca
     6361 gcgaacgcat cagttcttcc ctggagacgg gtctttttgg atttttcatc aatattttta
     6421 aaattccaaa ttcatttccg gacagcttca gctctttctc cttgtaatgc agaacggctt
     6481 ctgtcagatc caatgtcacg tttccgacac agcagagtgc tttttccgat acaaatgcat
     6541 aagaacgacg aagcactgcc tgtattttgg ctacaaccac ttcnnnnnnn nnnaattaca
     6601 gatctgcaaa agaaaggaat gattttttat gttgattttt acaagcgaaa actttgaaaa
     6661 agaagtactt caatcagata aacctgttct tgttgatttt tttgccacct ggtgcggccc
     6721 gtgcaaggct atgcatccgg ttctggaaga gcttgacctg gaagaagatt ttaaggtcgg
     6781 acaggtcgat attgatgagt acccggaact ggtaagcaaa tatcgcatca tgtccgtccc
     6841 gttctttatt gtatttaaaa atggagaagt tgccggaaaa acgatcggag ttcagtcaaa
     6901 agaagagctt ctggcactga tccgcggctg ataaatatta ccttgatttt gaaaaagagg
     6961 atacttttcc ggtatcctct tttatgcaaa aagttttctc cacaatctca aaaatccgga
     7021 tcggatctga taatcatctt cccgccagtc aaacagactt tcgtattctt cttcggtgag
     7081 gatgtttttc agttcctgtt ttttctcatc cggcacgatg acatttacac tgtcagcaaa
     7141 gcagagaccg gggtagagta cacaccacca gtttctgcct tttgctttcc cgatgcagac
     7201 tcgcagtgcc tcataagttc ctgccggaaa ggtgcagtcc ccataggttt tcatcggaaa
     7261 atagcacttt tccagattta cactcaccgg ataagtatag ccctgtttct ggatttcctg
     7321 ttctgccaca agacagatcg tttccctatt ttctgtgata cgtttttttg tttccttaac
     7381 atcttctgct ccgtccagaa tgtctttcat ataatctacc agccggtctt tgacttccag
     7441 tttcagtttc tggtcttctt cgctgtcgct gtttgccagc acatgaaaac gcaggatttc
     7501 ctttgcgatc ccctgttgga tctgatctgc tgtctctttt gttgtctgtt tctctattcc
     7561 atggattgtt tttgttgttt ttgttttctt tgccgtattt tcgttttctg ttttctttaa
     7621 aacattgaga tagttttgcg gcacaagttt gccatcctct tctttgagct ttaaaatttt
     7681 atgttccccg cttccgttgt gcagacggta cagaaggctt cccagtgtgg cagcttctct
     7741 tttatggtct gtgcttttct gataaaatcc ggttaaatcc tgcgattctt ctttctgttt
     7801 ctcctgctgt tccagtgctt tgccatctgc tgtgaaaaaa tagatatttc tcgaaaaagc
     7861 cgggcgtttt tccagtgcca gcaatacttc tcccagtttt tcatcatcct ctgccagatc
     7921 atcttccagc aggacacagc gtacctgtcc ataatccagg tatttgctgc ttttatcctg
     7981 ctctgtctgc aaagattttt caaacgggac cgcactgtcc cggatcacta gtgtatccgg
     8041 aaattcccgc tcctccagct ctcttccgca gctgttaaga caaaggagca ctaaaacact
     8101 cagaataccc aggattgctt tccgggttct gtttccgtac ttccggtctg tttttttgtc
     8161 ttctttttgt tttatcctct ttttcttcat actctttccc ttctttcttg ctcaaaacgc
     8221 tatctgcaac aaagagcaat ggtcctagat attatttttt actttcttta tttgcagtgt
     8281 atttttctta taattttcaa taacgccacc agcatagtga tcccaagaaa tcccaggctc
     8341 accagctcat acagctgtac acttttttca taagacgggc ttacgcacgc cgcagcactt
     8401 gctgccgctg ccatcagcca ccacttccac ttcttctttt ttccgggcag aagtcgttca
     8461 ctgcaagtcc caagaacggt gaatcctgat accaatgccc cacagaccat gccaagaaag
     8521 aacactgcaa ataaaacatc ccaccgctgc aaaaaaatcc ccttaatttt ggaattgctc
     8581 atcgcagtaa tgacagaaaa tggtagggca cggatgccgc cggctccaaa aacagcctgt
     8641 aaaagaaaca aaagcaacag acttccgatc agcatgctac ctgttgtttt ctcgaaaaca
     8701 cttctacttt gctgctcttt ggttgctctt tgcagaaata catcagaccc cagacctgca
     8761 aaaacagaca gcatggcgat acctgcgatc catatgctac ccggagcaga tggctgcatc
     8821 atttctccaa gtgtcccaaa ctgtccgcct cggatcgtaa gcaccgccag aaccagaaga
     8881 agcagcatta ccggaaaggt caggatcgga cagagcactt ccagaaattt tgcccagact
     8941 tccagtctgt gatctgccgc aaaactgcag acggctgccg ccggcaaaat cagcatccat
     9001 attttctgtt ccggcagaag gaaacactgt gccacttcag caaacagaca aagtaaaaaa
     9061 aatacatctg ccaaaccgta gatcccatat acaacggtca ggattgcgat ttccgcctgg
     9121 cctatgccat cctttctgtt ttctgctgct ttgttctcca ttcctttttt tctgtctgtg
     9181 gcaggata
//
LOCUS       DAWDWH010000070         9168 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3749_length_9168_cov_15.631625, whole genome shotgun sequence.
ACCESSION   DAWDWH010000070 DAWDWH010000000
VERSION     DAWDWH010000070.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9168)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9168)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9168
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3749_length_9168_cov_15.631625"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2126 a         2331 c         1715 g         2996 t
ORIGIN      
        1 cattgttttt tgcccccatg atcccggcga tgcgggcaat tccaagtaca tctccctttt
       61 ttgccgttcc ctgagaaatt gccgcaaaag catcctcatt tacaaaaatg gtgccctccg
      121 ctttcgccac acggtatgta tctgcctttt catggacatc caccatatgt gcctcgccgc
      181 tttcgttgaa atgtgtcagt ttcttttcat tcatactgtt tctatccgtc ctgttaatct
      241 gtttcgacta gtttatccgc attatcctca ttttaaacca tccgtcagat tgtttcacct
      301 gtgttcgact cgattttagc tgcacgtatt tacatgacga caaaccgctt ccagcacgct
      361 gccgccgata cttctggctt tatctgaaat cgtaaaacag tttttcaatt cgccttctct
      421 tggatcaata tctgcgatct taaatccttt cggaaaatga tagccttccc ggatcagacc
      481 gcggataatg ccggtgattg ttgccggaac tgctaccgat gcaccgctaa aagttccgga
      541 ttcatccatg ttttcataaa ttctggcaat ctctgtgccc gcttccacta catcgccgat
      601 ctggtggata tttttgataa atccttccgt cggtgcatgg atcacgcgtt cttttgtaaa
      661 tccaccgata ttgcccggaa tcccggtatt cggaagtgcc gatccttctt ctaaaattct
      721 tgccagatta tgtccacgca tcgattcgat cacaagatcc acatcgaccc cggccgtaaa
      781 tccaggcccg actccgatca caagcggagc catctctttt gttgttccaa ggtttttctt
      841 ggcaatgatc gcatctacca cgacatccgg atgcagctct gcaatcgaca ctcctttgga
      901 atcaacgaaa agcggtacac atcctccggc aaaaacttct tccatttcag atacagaatc
      961 aatcctctgt gccgtcacgc cttccacggt cgcttttcct tcgtagaccg cctcacagaa
     1021 cgaaacctga cggcggatcg ccgccggatg atccgtttcc agcaccagca cacgaaatcc
     1081 cgcccggttc agacggtgta cgatccctgt cgccagatcc cctccgccgc ggcagatgat
     1141 caaactattc cgtttcattt taatcctcca tttgttccgc tcagactttc gaaaaacccg
     1201 gtttcaagaa tgcctttggc attcttgctg caaggtacac gtcatgcatc gcatgacata
     1261 ttcttctgtg cacctctgca atccgccgga ggctctgtcg gtatctgcct gacagaatcg
     1321 aatgttttcc ccatcttgct ttgaaaacag acaagttatt ttctcattat acccgatttc
     1381 tttcagcctg taccgatacg cccttaagat tctgcggata tgctcttctg tcactgcttc
     1441 ttccggcaga atctttcttt ttggcagctc catatcatcc cggataagct cttctttctg
     1501 catctcatca aagcgaaaga ttaccttctt acagggtttc ccaatatccc acgttccaag
     1561 cacgagataa agatccgtcg ttcccgaaaa aatcaccggt tctgccccgg aaggatattt
     1621 cagagaatga tgtttcgctc cgtccgcttc tatcagagca atatccgcat aatgcagcaa
     1681 ttttcttaat gtatgctccg gaagtgcctg cattttctgc ggattttcgg agcattgtat
     1741 gcccgccata caatagtgat ctctctgtaa tttctcccgt atagaaaaag catcgccgga
     1801 gaggtctgta cccggctctg cgaacatatg cgttgtcgtc gtcaccagca cagcatttcc
     1861 tttcctttga tattcttctg ccagtcgatg gatcagggtc gtctttccgc ccgctccgat
     1921 gactgatatg atttttttca tttatttatc ttccagttcc cacatttcac gaaccggttt
     1981 ggattctttt ggcagaacga tatttaaaat gatcgctgcc agagaacatg gtacgattcc
     2041 ggaaccgcca aagatcagct gtacataata cggcagatga gcggttgccc ctgtggttgc
     2101 tccaagacca tatccaagac cgatcgctac ggaaataatc gtaatctcac ggctgccgat
     2161 cggatgcttt gtgatcagct ggataccgga aatcagaatc gatgcgaaca tcataacggc
     2221 tgcccctccg agaaccgact gcggcatgat ggacatcagt gcaccgatct tcgggcagaa
     2281 tccacacagg attaggaaaa tcgctcccat ggagattgca aaacggttga caacttttgt
     2341 cagagaaacc agtcccacat tctgcgagaa tgaagtattc ggcagtacac cgaacagtgc
     2401 cgcgaaagcg gatccgagtc cgtcacagat cacaccgccg gacaactcgg aatcggttgc
     2461 ttcacgatcc ataccgccct ctatacaggc cgaaatatca ccgactgtct caatcgccgt
     2521 tacgataaac atgatgccga tcgggatgat cgcacttgcc tggaatgtga tctttacatc
     2581 agataaagca ccaaaaccaa tgaaactcgg cacagcgatc cacgctgcat tttttacctg
     2641 ggcaaaatca acaacccagg agtaagtata ctgaacacca tctgcatcga taccggtgtg
     2701 cggaagcgta agtgtcataa tgatcgccac aacatatcct gccagaatac caaacaggat
     2761 cgcagacgaa gaaaggatac ttttctgcgg tgcgaaatgt ttcagcagta cgatcacaac
     2821 cagtactaca aatcccagaa acagattcgg aagtgaacca tagtccgctt tgccgcttcc
     2881 tccgcagaaa tagttgatac caacaccaag cagtgataat ccgattgcaa taacaaccga
     2941 tccggtaacg atcggcggga aaaatttacg aagcggtttc aaaaatgctc cgagaacact
     3001 ctcgaaaata ccgccgacaa tacaggctcc cataatcgca ccataagcaa gaataccgcc
     3061 gccgcatgct gccacggtat tgttcagaac tccgagaaat ccggatgacg ttcccataac
     3121 gatcggcact tttcctccga ccggaccgat ggaaaacatc tgaaccagtg taacaactcc
     3181 ggcgatgatc attgcattct ggagcaatgc cgtacgaagt tcgccaaatc ctgcatctgc
     3241 ggtcagtcca caaactccca tcacgatgat cagcggtgtc aggtttccga taaacattgc
     3301 catgacatgc tgaagcccca gtggaattgc ctgagaaagt ggaattcttc cctcaaaatc
     3361 atacggtgaa gaataacctt ctgacttttt cttatttgtt gctttcataa aacaaacagc
     3421 ctccttaaat tttacatata ttccatgttt ctttcttatt ttacaaagat atatttccgg
     3481 ggtcaattcc taattttact ttttttcaga tgcaaaaatt cccgcccatc ctgaccggat
     3541 ggcgggaatt tttgttctac ctgactttta ctttttttac tttactaaag gaaccgtaaa
     3601 tcctgctttt ctttccatct ttttttacat tccggaattt ccgcattctc acatagtact
     3661 tcttttttgc tttcagtttc gacaccgtaa gcgaattctt tttctttcca cacttctttg
     3721 ttttgacatt ctttttaaac tttttatccg ttgaatactg gatctgataa ccggaaactg
     3781 actccgattt ctttgctttt ttccatttta ctgtaaattt tttctttccg gctgtcagtt
     3841 ttttgatgga aataccggaa agtttctgac catccttcga atttacatcc ggtatctgtt
     3901 ctctgttctc tgtttccttt tctgtttttg tttcctcttc tgtttttgtt tctttttccg
     3961 tttctgtttc cttcggtttt tctttccatt ttgcataaac ctggatatca tcccggtact
     4021 ttggaaccca gtcatagttt tccgtatgtt tgagcagact gccatctaag tcatccggtt
     4081 tcagacacca tccggcaaac tgatacccct cctgctgcgg aatgtgatct gcaatatcaa
     4141 aaaagttttc actgtccata tgcaattcat aaatgacaga ctcttttccc tcaatacttc
     4201 cgccatttgc gttaaatgta attgtttttc cgctgtcata tctcggtgtc actgtctcat
     4261 cacctaaatt tgcatgaatc atattgtgtt cccagtcaaa ggagaatatg gttcttccct
     4321 cttccacttt ttcatccaca aaagccatac cgctccatcc agtcagatga caccctgcac
     4381 gaaccggtgt ttttaaaatc agttcctgtt tttccctctg cacataataa cgatccggca
     4441 tgccttcctg ttcttcttct gaaagttctt tgtaggatat agtataatac ggttcataga
     4501 attggatcgt acagtttgaa ttatgcgtgg tatacccgac aatcatcggt gtactgctat
     4561 attcaccacc ataaatggtt ccttcatgtt cggtagctcc tgtacgcact tgttccaaaa
     4621 caattggggt gtttgtattg tttgtgtaac ccgcattctc tgtgctctcg ccttcctgaa
     4681 aggctttaag ctgcttctgt gtatcttcac ccatctctgc actttccaga ctcttcttcc
     4741 agagctccgt ctgtgttgtc cagacattat acgtgtttgt ccgatccgcc gtcagtccgg
     4801 caagatccac tttgttccgt atcagtttac tgtcataaag tcttggttct tccccaagta
     4861 tcagggaaac gtgtttcagg tacaggttcg taactgtctg cccgtaataa ccatggtcat
     4921 acgttacttc aaatttcgga acggtgtaaa atgcttcgcc cggcataatc agctcatttg
     4981 aaatatccca gttattttct tccttcagtg gatggtccat ccgcatatac gttggctctt
     5041 tttttgtttc ttccgcaaaa gaaaccggca ctgccagatt ggtcagcata cagcatgcca
     5101 ttgaaacacc gattattttt cgaaaaccgc ctttttgttt tttcataata aatccctcct
     5161 gacatatttt ctgtttttct gatttttctt cccgttttct gagttacttt tattatatca
     5221 ggaaagattt tttaaaccac aaaattccca aactgtccag tgacaatttg ggaattcgtt
     5281 catttgcaca tgctctgctg ttttttcgct gacagcctcc tgataaaatg ctcaggattt
     5341 caatttctta cacctgccag gaagtcaccg acaacttctt atttagtaaa ggtgacggta
     5401 cagtcttcat atccttctgc ggaaatctca atggtatttt ctccatctgc aactgcattt
     5461 gttccaacat aaatctgacc atcgtttttc agcagacagt aggttgtcgt atcacctacg
     5521 ctgaaggaag aagttaccag gctatactct gttccgttta cttttatgcc tgtgatctga
     5581 ttctgccact gttcgatttc agaaccatag ccaaaggaaa cgatatagta atctgagaag
     5641 atagttttct ggcaggaaac cgcctgtggc ggtgtcagtt ttttgatcac atttacaacc
     5701 agcggcagtg ccagatcttt atagccttct gctttgatcg taaaggtatt gtcggcattt
     5761 tcatcaaatt cgctgtccac tttcagtttc aggacctgat ttccgtatcc gtctgtttcc
     5821 catttaaact gattctctga catgccaaac cagcctgctt ctgtataggc tgttccattt
     5881 acagtgatcg aggttacttt atttttccag cctacaaagt ttgtctgtgt tccaaaggat
     5941 accagataag tctggccgtc ttcgtctttg ctgatcatag gctctgatgc cgaaacatct
     6001 ttttcttcac cctgtacggt gatctctgtt tccagagctt cataatattc tgcttccaga
     6061 gtcaaggtat atgctttgtt ctgattgaac aggtttccgt tatcgtaaat agtgatggat
     6121 ttcagatctt ctgctgcttc atagtaataa tcagatcctg caacaccttt tgcataaatg
     6181 ttttttccat ctactttgac tgcatttaag ttcttcagga aatcgccttc ggaaccattt
     6241 acggtcagaa ccacattctc gcctctgttg tactgttctt tgtcagaaac aagacttaaa
     6301 ttctcttcca gaactttttt atactcaacc gaaagtgtat tgtttttgaa tccttctgct
     6361 ttgatctcta cggtatttgt tcccagtgtc agaacagaat ctttgatcac cagtttgccg
     6421 tcctctacgc tgtactgatc ttctgtcagt tctttccagc tctgattcac ataaattccg
     6481 gtaattttct caaagtattc tgctgttctt gcatcctggc ctgtcagtac aagatctttg
     6541 ccttctgcaa caaattctgc atccgcatcc agtgtcagga ccggtgcttc ttttcctttg
     6601 taagatccgt tgtactgtgt ttctccaagc agaccatctt ccagaaccac cttaactgcg
     6661 tatggacggt tttctgtagt aactgcattt tcatctgcaa cataatcttc aaaagagaga
     6721 tactgtcctt ttaattttgc ttcctgaaca gcatcatagt agcttgtata atcgtagaat
     6781 acaactccgt cttcgccata aacggcatcg catgctgtga tctgattatc ccagcgctct
     6841 gcaactgctt ctaccgaagc attcttcaca tccagtgccg tcaggatctt tgcattggag
     6901 atcagatcct cagaaaatgc cagatttgcc ggcataacag agtttccgct ttcaccgtcg
     6961 ctgctgcttc cggagtttcc gcctgaagtc gctcttgtca caacatcata agtctttgct
     7021 gtttttgcag cttttgttgt tttgcctgca acagtgaaag atttgctcat ggtcttaaag
     7081 ccatttgagt ataccgtcag tgtatacaca cccggctgtt cgatattgtt tcttccacct
     7141 tctgcattta catcgttata cagtacaaag gtatttccga tcagatacca gtctgtgatt
     7201 ttttccagtg tttttacttc tcctgacgga gttttcagtg ttaccatctc aaccggaacg
     7261 gtgatgccgt ataccatgtt ttctacctgg aaatgaacat tctgtccgga tttgatcgca
     7321 cctgtctcag aaatcttcat ggacggagtc tgtgcattta ccacatggat ggatgcgatc
     7381 agggttctgt ttccattgga acggatgcgt actttgtaac gtccattgga atagaaattg
     7441 ctctgtccca gcggaatggt aattcttccg actacatttc caccatgttc ggtggtatct
     7501 tttgtataag cgagctggtc gttcagcgtg ctaaggctct catcgtttga cagcagtgcc
     7561 agtgcacctt cgtctgcgat cgtatcaaac cagtcttttt cttcctgtgt tgtgtagttg
     7621 aacaggattt ctacgtttcc gctcacaccg tacagattgt cagcatcttc gtttttgctc
     7681 aattctgcat ccggtgcata atatttcaca tcttcttttg cagtttcctt caggtcaaag
     7741 gttgtttttt tcggagtaac ccttgcattt ccttcttcat cgtagttggt cagatgataa
     7801 tcccaggtcg ggatagctgc atgtgtcagg aaatacatcg gttcctgtgc ttttacaacc
     7861 ggtttgtccg gattttcatt gtcactgagc acaacatcct gtgttttctg gtcatctttt
     7921 ttggtaacaa tcagttttgc aggatttaag cctgtcagct cccatttcac aatgcttccg
     7981 tcatcggtaa cttttgtcat agcccctgtg atatccacac catctaccgc aacgctgcag
     8041 ttttccacat cgtaaccttc ttcaaaagct accaccagat actgtgcata tccaagatca
     8101 agaacttttg tctgagcttc ctttaccaga actgttttcg gagcttctgt ttccgttgcc
     8161 ggtgtttcgg tttccggtgt acttggcgtt gccggtgtct cagttgccgg tgtctcagtt
     8221 gccggtgtct ccggtgtact tggcgttgcc ggtgtctccg gtgttttatt attatcgact
     8281 gccggcttct ctggcagaac tactttaatc ttgcagcgat attttttgct gccaactttt
     8341 gcagtaatca cagcatttcc ttttttcttt gctgtaacct ttccttttga agtaacggat
     8401 gcaactttct ttttcgagct gctccatttc acttttttct ttgtgccttt taccttaagc
     8461 tttaaggtct ggcctttgct cagtgttttc tttttgtaat tgagcgtggt tttggacgct
     8521 gctgaaacgg tatgatactt tggtgtaacg ttagcaaaaa ttaccgcaaa actcagcaga
     8581 gccaatacca gcaaattctt tttccatcgc ttcatcctct tttttcctcc atggctatct
     8641 tttgttagtt tacttaaact tgcgtttgaa taaggtaaca tattttctca ttttcatcaa
     8701 gtgtaaatgt gcttaacaag aaattttctc attgactttt cgtttgttga cagccatgcc
     8761 atcgccgggc tttttattat gacactcggt gtttcgatgt ccgtaaaatc gaacctcggc
     8821 gtttctccgg tcagctcgat tccgtaggta tcggaacggt ggtcgctact gttctcgtca
     8881 gattgatcct cggacaagaa gcatctctcg ctctaaagag ctcggtcact ttttatttgc
     8941 tatttatcac caatccattg ttttgcaagt tctacatcta cttgcagtgc cattgcaatt
     9001 tgttcaacag gcaatccaag tctgctaagg gcaatcgtac cctctttttg tgcaagtcgt
     9061 ttgccttccg caatgccaga ttcaacaccg gcctcgtact cagcttttcg cagtttccgt
     9121 tcttcctctt ctttgtcata ttcaaaaata ctcatcgcaa ttacctcc
//
LOCUS       DAWDWH010000071         9114 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3773_length_9114_cov_18.755823, whole genome shotgun sequence.
ACCESSION   DAWDWH010000071 DAWDWH010000000
VERSION     DAWDWH010000071.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9114)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9114)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9114
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3773_length_9114_cov_18.755823"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2216 a         2127 c         1693 g         3078 t
ORIGIN      
        1 actttcttca ggtttacggt ctttaagttc ttatcaccag agaaagcact ctgtccgatg
       61 gtcttcagtt tgtttccgtt taccgtaacg gttttcagtt tcgtgcaatt tgcaaatgcc
      121 ttctttccga tcttttctac gttgctgccg atgcttacgg aagttacttt tttattacct
      181 ttcagggcat tggctgcaat agcggttact ttataagttt taccgtttgc tttgatgttt
      241 tttgcgattt tcacgctgct ctttgctttg ccttttgcag tcatgttttt cacttcaacc
      301 gtatcatttc cggtaacttt gtagttaaag ataccggatg catctgcgat cacgctgccg
      361 attgccgggg tctggctgtt gttatttcca ttattaccgt tgttgttgtc gttattatta
      421 tccggtttct ttgcttccag tgctttcatg gcgatgtcta atgctctgca gcatctttca
      481 gctgttttgc ggttgctgtt tcagaagcaa atgccatttc tgcatccctt tttgcttttg
      541 caaaatttgc ccagctttct tttgtataat ctgcttcttt cttttctgcc gcctgtgcaa
      601 tctttgtctg tacttctgtt ttgccggagg cttttacttc atcgagtttg cccagtgctt
      661 tctttaaagc atcgtctgct tctttgattt ccagtttggt tactttttct ttctgtgcaa
      721 cttcttttgc ttttgcgatt gcctctgcga taccggactt ggcaaaagtt ccttccggat
      781 aatctttttc cagataagtt ctctctgcaa cctgtgcatc ggtacttaag gtcgctttat
      841 cgtttactgc tgccggctca tcgcatacga tattgtatgt cacaccgtcg atcaccgttg
      901 tggttcttcc gatctcgttt gctttgattg tcacggttgc ttccgtcggt gttattcgtt
      961 tctctgctgc tccaagaagt tttgtctgat ttcctgttcc attcgttggg tagagaacaa
     1021 taacatggtc tttattgtct ttattaaaga tattggcgat cagatatttt ttaccggaag
     1081 taacttttga tactctctga tatcccggaa caatatctcc tgcttctgct ttgtcctttt
     1141 tctcaagcag aaccacctca taggtcccag ctgtaaagct tgcattatag agtctgtttg
     1201 cgttgaagtc catgtttgta taatagaaaa ttacatatcg ttctccgttt tctttgcaga
     1261 tacggaaagt atcactgcca tctgtcggcg ttaccagcat attatttttt gctgctgcac
     1321 tgaaaaatga atttgcaccg ctttcattgg tgagatatac ctttttattt tcattatata
     1381 ctgcatacac atttgctgtt ccggttgaag taaaggtaaa ctctgcatct gtcagattca
     1441 gtgatatatc attttttgct gcaaaactgc tcaggtcaga attattgttt gctatatgtg
     1501 catacatcgg cacaacttct ttttctacac catctacttt tgtataattt acatctgcaa
     1561 ccttttgatc tgcttccgtc gtaatacctg ttttatgttc ttctgccacc tggtaagacc
     1621 ttgtataagt ttcatttctg gtaagttcta caccaagctc taaatcattt atataaccat
     1681 ctgccgcaag ctgggcgata tcataattat catagcgtat gcttcctccg ccattttccc
     1741 agagaagtcc taccgttccg tttttcagct ctgacataca ggaatactga taagtgccgc
     1801 tgtttactga atagctgttt tttaaagtca gcgtcttgtc ttcttctacc agaaatgtaa
     1861 atattttacc gtttgctcgt ccacttccgc ccggggcagc taccaggatt gcctgttttc
     1921 catcaatcgt tttagaataa ctgatcgctg tcacattaca ggttgatgta caggaaacgc
     1981 ctgtcttgac cggctttgta aatacataat caccggatgt atttcttact gcatctgaat
     2041 agcagaccac tccctgtccg cttctgaaga acatacgcaa tgttccgtct gaaagctcta
     2101 cgacttcact ttcggatgac cagtaaccac ccgcggctgc tcctggtacg tcattggatc
     2161 tttcccatgt ttttccatta tcatcagacc agataatgct tgcattttct tccccatcgc
     2221 catgatcata gaatggaatg acgatcgtac catctgaagt cagtagcccc tgccccggag
     2281 ataccagcag tgctgtctct tttgtcggac gtttgatctg tggattcagg atttccggat
     2341 ctgtccaggt aaatccatca tcgtatgttt ttgcatacat catatatccg gtattgtata
     2401 catgcaatgc agatccttta tagaaaatat tctgctgaat atccgtattg gaattaacct
     2461 gtttctgtgt cagggtatct ttatactctc cattttctac tttatacagg ttgtaccact
     2521 tatccacgcc atatcctgtt tccgaatgat cggatcgttt taaaattttt gcatatccgt
     2581 tctcatccca gtcccctaca taataagcat aagttgtgac atcgtcatct tttggctgtg
     2641 tgtttacatt gtcataatta tccgttactg ccagataatc ttttccgttt actttaacat
     2701 aaccggttcc ttctcccgga gctttataac cgcccattgt cgttacgccg gttgggttta
     2761 catcggcaat acaataaatt tctccgtctt ttccctgtac cagtgccggg tcgatgatcg
     2821 tagttgccgt attgccgata taaccatcgc tgtccggaaa atacagtggg aatgaataat
     2881 tccaggtctt accattatcg gttgatacag aagcgatcgt atccagtccg ccgccgtcac
     2941 cggttgtaga atatctcgca tccgctgctg ccagaagatt tcccttctgc gtaacgataa
     3001 atgccgggat acggaaatac tgacttcccg ctgttctctc tgcaaacggc tggttctgtg
     3061 ttacatgctc tgctgtcgtg ttgatcggac ctgctttcag caagtctgct gcgtctgctt
     3121 tcgctgccgg aaacacagct actgcaagac cacaaacaag agctgttcca agcccctgtt
     3181 tccacagttt tcttttttcg ttctttttca tagtttcctc cttattttcc atcctgtgat
     3241 tattcaattt tactatattt ttactgtttt gtccagcaaa aaaaaccttc ctacagcact
     3301 tgcatgctat agaaaggttc gccgctcttt ttataatctt tatattctca aattttgatt
     3361 agtcctgaac gtatggcata agagcgatgt gacgtgctct cttgatagct actgtcagag
     3421 ctctctgatg ttttgcacag tttcctgtga ttcttctcgg aaggattttt cctctttcag
     3481 agacatatct cttcagtttg tttacatcct tgtaatcgat ttcgttgttt tctttaccac
     3541 agaaaacgca gacttttttt cttctgcgtc cgcctcttct cttcatcgga gaatctggtc
     3601 tttcagattt attgtaagcc attgctgtaa acctccttta atgtctagtt aaacggtaat
     3661 tcttcgtcaa taccgtccgg gatattcata aatccatcgc cgctggctgc actcggttcc
     3721 ggtcttgcag gtgtatagtt atttccattt gcctgagacg cagctttgct ttcagcaaac
     3781 tcctgatcct ctactactac atctgtagtg taaaccttgt tgccatctct gttggtatag
     3841 cttcctgtct ggatacgacc tgtaacgact accttcagtc cctgatggaa atacttctcc
     3901 gcaaactctg cactgcgtcc gaacgctaca cagctgataa aatcagcggt tgcttcgcca
     3961 tcgcgtttgt tgaatctgcg atctacagcc aatgtatatc ttgcaactga tgtgccgctc
     4021 tcccctgcgg aataacgaac atccggatct ctggtcaaac gtcccattaa aataacttta
     4081 ttcattctaa atctccttat ttatgcttcc tgtcttacgc ataaatatct gataacattt
     4141 tccatgatac ggacatgtct ttcgatttct gccggagctg tagcatcagc ttcgaactga
     4201 atgaaatagt agaatccttc tttcatcttc tggatttcgt aagctaatct ctttttaccc
     4261 cattcgtcaa cattagtaac tgttccgccg taacgtgcaa cgtattcttt tactttttct
     4321 acagttgcta ctcttacgtc atcctcaatt tttgcgttaa caacaacggt taattcgtat
     4381 ttgttcatgc tcttcgcacc tccttatggt ctctggccca ctattacctt cgtgagcaag
     4441 gaattcaatg tttcaaaatg gctgcttgtc tgcagccact cacaattttt gattttatca
     4501 tatttttgca agacaggcaa gtattttttc ttattttttg caatgtttcc gcctcgcagc
     4561 caaacctgac tttgaagcag aatcatgatc taattcacat atttcgctat tacatcccat
     4621 atggatgatc tttcaaatcc aagcttccac tctgccactc cggccaggcc atattcctgc
     4681 acaagtttca tcttctcttc gattgatgct tcgttttcca gccagatctg acagaaattg
     4741 ccatcttcat ctgtgaactc tgcataatcc tgtgatgtct cgctgctcca tccggcagtc
     4801 accttgtaat cggacaagat ctggtctgct ttatccatgc cgatcgcctc actgcttact
     4861 tcaccggaag cattggtctt ccataatctc gtgtagaacg gaataccgct gatgatcttt
     4921 tctttcggaa cgttttccag tgttccggta atgccgtctc tttcatagga aagggatgcc
     4981 acggaaccgg catcggaacc gacatagtgt tcatcatagc ccataatgat cacatagtct
     5041 acgacttcgc caagctcttt ctggctgtaa tgttcattga aactgtatgg cggcgggaca
     5101 tccaccgaaa gtacaagatt gttattcctg cactttacag agagttccct cataaactgc
     5161 acatagcttt cggaagtttc ttccggtaag gattcgatat cgatattaat gccatccagc
     5221 tgatagttga gtgcttcggt aatcagctgt ccttccaggt tctctctgga tgtggtagtg
     5281 ccaagcacag tgtcggtatc aatatccgta ctgaaattgt ccatcagtcc ccagacttcc
     5341 atatcattct gatgtgcggt atctacatag tcactgcttg caagagaaga aatatctcca
     5401 tcgttgctgc tgatagaaaa ccaggtcggt gaaatgacat tgaccccttt tacatcctga
     5461 atgtccgctg caagtgcatc gtttgaatcc tggctcgtca cctgatgcca gagaagattc
     5521 atgtcataat ctttatgtat gctggtgtac tctgccgccc ggaaatcact ggtgacctgt
     5581 tcttcgtagc tgtcagaaag ctttttgctc ttcatatagc caatatatcc atccgcagtc
     5641 agaacttttg tccactcttc ttcctgttcc agaaccgtca ctttatctcc gaccgcttct
     5701 ttttttagaa tcggactttt gatatctgca tccagacgta cgctttcttc ttttatcaca
     5761 tccgaaaagg ttttcattcc ccactgattg tttataataa tatgaagcgg ttcctgctcc
     5821 accgaatact gaagtgctgt atactgtgcc agataatcca gtgcaatata gacatttcca
     5881 tcctgcgaaa caacgatctg atacggcatg ctgccatttc cggtaagcgt tgtatattca
     5941 ttgctgtcat acggaagttt gataatatcc actgccgttg tcagtatgac ctgctgttct
     6001 tcatcgcttt tataaagatg atcattcaga ttttcttttg cagtcgcata ttctacatat
     6061 gccgtgccat ccttcacaag tgctttcccc tgttccagac ggttcggaag tacgactgct
     6121 gcatcctccc ctccggaagt gccaaaatat tcattgccat ccatcacttc tttggatggg
     6181 atatatcgat ttacaacttt atatcctacg cctgccgcag ccaccgccag gatcagcggc
     6241 actacgacct tcacaggact tttctttttc ttcattcatt gtacctccat actatgatgc
     6301 ctgcggattg ttccgtgcgg tgcactccca caatccgcct tagtattcgc atactgcaac
     6361 atcgttttta caacggaata catcgttttg attcagtgta tcatagattt atgaacgaaa
     6421 gatgacttaa gtctacattt tttataatcc ttcgacctgt atggttgtct tctgtactat
     6481 ggggatttgt catcagacct ctttcttttt tatatagtcg aatccgattc ctataatgtg
     6541 atcgtcatca tcgcttacaa aatctctcat atactcctct tcttcccgga tcatgcactg
     6601 gtgcatgata tccgaaggtg tacttggctt tccattcaac cacagcttca tgcctgctct
     6661 ggcatatgca tttaattctt tttgcagtcg atgtttccgt ttttttctgc tgcctatagt
     6721 tccaccccct cctgttaaac ttaaagattc tgctgacatt atcgtgagaa tttaaatcga
     6781 atacacataa ggctgctgcc tgacatgcaa ttccggtcag cattgctgac acgaaaacaa
     6841 caccgtatac gaaatctgta aatcagaaac attcaagcct ttttctattc ttttgcaaag
     6901 tgcagtacgt tcttttttgt tccgatcaac agtacttcag gataccagat aacattcatt
     6961 gtacgaatgc aaagtaccaa agagtttaca tggcatcttt taagctcaat attgctttat
     7021 ggaggtacaa tcattttttt ggaatattgc ggagaatccc gcataagata ataaaaagcg
     7081 acagaattcg ctttcttatt cattttgatt tttgatgttg cttaaagcat tgtttcatcc
     7141 tcacattgat tattatatac tttatgtttg aaaaatgcaa gatgttttta cactcttttt
     7201 ataattttac actggcaatc tactttactt tttgatagta tgcagatata attataagat
     7261 aataagatgc caaggtcaaa agtctgaaga cacaatgtgc ttgcacagga gtggttttat
     7321 ggcatctttt aaccttaatc aatatcataa tcatagtaat tccaataaaa ggttaacata
     7381 tcatagatag gatgtgataa tatgaagata gaaaaaatca acgataatca gattcgctgc
     7441 acgcttacac gtcaggatct ggcagaccgt gagattaagc tcagtgaact tgcttacggc
     7501 acagaaaaag caaagctgct gtttcgtgat atgatgcagc aggccgcata cgaatttggt
     7561 tttgaagcaa atgacattcc tttgatgata gaagcgatcc cgatttcttc cgactctgta
     7621 attctgatcg ttaccaaggt ggaagatccg gaagaacttg atacacgttt tgcaaaattt
     7681 tcaccgtcaa acggcgaaga aaacaacaac atcactccgc cggcaccggc acctcttgaa
     7741 ggagccgatg atatcctgaa tcttttcaat aagatccacg aggcaagaaa gatggctgaa
     7801 aatgcagcag caaaaacttc tgaaaagaaa gaaaaggctc ctgcaaaacc ggagatcaat
     7861 ccggaatcct ttcatctgac gaaactttac cgtttccgcg atctggacac tgtgatccgt
     7921 gcagcacaca ttctgcaaaa ttcttaccag ggttacaaca cgctctataa gaatccggaa
     7981 ggccgtgaat atctgctgac actcagcaaa tctgcacaca caccggaaga gtttaataaa
     8041 gtctgcaata ccctttccga gtatgcttcc agcctgaaat acacaccggg caacgaagca
     8101 ttctttaaag aacatctgga atgtatcgtt gcggatgagg ctctgcagaa gcttgcattg
     8161 atctgattga tatgaaatcg atagtcagag tttctgaacc gattcgctgc aaacggaaat
     8221 agagtgtaga aaaagcgtct ccgcctggaa aatcctggtg gaaacgcttt ttattttttt
     8281 atggatattc acaatccact tttgctgtct gtttatgcct gtttgctttc aacaaatgca
     8341 tttactcttg ctaccagcag atccggctcg ataccatgaa cgagagctgc ctcttcgatg
     8401 gtctccatct gtgcggatgg gcatccaata cagtgcatgc cgcctgctac cagtacgcct
     8461 gccatttcag gatccattct taaaatatcg ccgataatca tatccttggt tattttctgc
     8521 atgattttcc tcctttatca ttctgtacag acttattttt ttcaagtcga acgcacgtga
     8581 gacttggcac gcgattctgg tcagctttgc tgacatttac ctaacagaat cgctgtgcat
     8641 cctcgcggag cgtttatctt agtcggagat tgcactccca ctaagacaaa tttgttgctc
     8701 gccacttata gaagtgggag tcttctccga ttaagataaa aatctgtacc tgatttcttt
     8761 tgacaggttg attataatat gtttttgtac tttatgcaag gcatagaaaa aacctcttgt
     8821 atattccaga cttatgtatt aaaatgggat aaagattcgg agagccgaat cctgttgaaa
     8881 acatatttca taaaaataag gaggttttat catggcatac gttattacag atgaatgtgt
     8941 agcttgcggt acatgtgcag aagcctgtcc ggcagaagct atcagcgaag gcgatggcaa
     9001 atacgtaatc gatgcagatg catgtctgga ttgcggtaca tgtgaaggag aatgtcctac
     9061 aggtgctatc gtagcaggtg aataattaaa aaattttata aaagcaaggg gagc
//
LOCUS       DAWDWH010000072         9113 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3775_length_9113_cov_14.982005, whole genome shotgun sequence.
ACCESSION   DAWDWH010000072 DAWDWH010000000
VERSION     DAWDWH010000072.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9113)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9113)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9113
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3775_length_9113_cov_14.982005"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         3137 a         1606 c         2113 g         2257 t
ORIGIN      
        1 ccggatgacg aaaaaattat cgcaaagaaa tttaagaaaa ttaaatttta taagactata
       61 ctactgacgg cttacagcga aatgggaaaa ggagctacct ggtatgcggc acttgcaaag
      121 cgagtcctga tcccgatctg caaacatctc aacacggaaa aactctgcaa taaattaaat
      181 gcgatatcaa aagagtatcc gatcgacaag tcgccgtatg tgggcggttt tctgtggggg
      241 tatggaccgc aggagaaaat gccgaaagaa tttttagagc cggtggaagt agaatttgaa
      301 ggacatatgt ttccggcacc aaaatgctgg gatttctatc tgacacaact gtacggagat
      361 tatatgcagc ttcctccgga aaataagagg atatcgcatg atttcaatgt ttatatgaag
      421 gagaataaag aataatgaat atcggactga tctttgccgg aggttccgga gtgaggatga
      481 atacaaaagg tctgccaaag cagttccttg agatgaacgg aaaagcaatc atcattcata
      541 cgatcgagca ttttgaaaaa tgtaaagcga ttgatgcaat tgtgattgta tgtctgaaag
      601 actatattga atatctggaa gatcttctgg accgggatcg tatcacaaag gtaaagaaaa
      661 ttgtgccggg cggttcctgc ggtcaggaat ccatctatca tggactttgt gcagcagaag
      721 agatttcaga gaatgaagat gacatcattc tgatccatga tggagtacgt cccctgatat
      781 ccggaaaatt aatccatgac aatatcaata tggtaaaaga aaagggaaat gcgatcacgg
      841 taacaccggc aacagagacg atcattcagg ttggcggtga acaggagatt acgaatatca
      901 tcgaccgcaa aagctgttat acagcaaaag caccgcagag tttttacctg aaagatattc
      961 ttgatgtgca tcgtcaggcg ataaaagaaa ataaaactaa caggattgat tctgcaagtc
     1021 tgatgaaaga atttggacat actcttcaca cactcgaagg gccgaccgaa aatatcaaga
     1081 ttacaaatcc atctgatttt tatattttcc gtgccatttt ggaagcaaag gaaaactcac
     1141 agatctttgg attatagatt atgggagaca gaaatatgcc agatattaaa atcagtataa
     1201 ttgttccggt atataattcg gagaaatatt tgccggaaac ggcacagagc attttaaaac
     1261 agtctttttc ggattttgaa ttaatccttg tggatgacgg atcaaaagat ggaagcggaa
     1321 aaatatgcga tcagcttgca gcgaaagata ccagaataaa agttatccat aagacaaacg
     1381 gcgggatctg ttctgcacga aatgcaggat taaaagaagc ggccggagaa tacattgcat
     1441 tttgtgataa tgatgacatc tacctgccgg gacttttaga agataattat gcacttgcaa
     1501 aaaaatatga tgcagatgtg gtgcgttatt ccagaagtta tcgcacggtc aaagacggca
     1561 aagtgatcgc ggaagaaaag acctctttta aagcaggtgt atacagcagt tcacagtttg
     1621 ctgcaaattt tgatcagatc aacaaagccg gagaaggaat ctgggccggt atttaccgaa
     1681 gagctttttt acagaaaaat cagattgatt ttgatgaaac catgaagttt ggatatgaag
     1741 atctggattt tataacgaaa atatatctgt gcagcccaag tgttgtactg aatccgaaga
     1801 cttactatgt gtggatcatg cgatatgcac acagtacaag cggaaagaca gacattaaca
     1861 atatcacttc gctgatgaag tgtctggaaa ataaaagcag actggtaaca cagaccggta
     1921 ttgaaacaca gattccggca ttttgggtgg aagagctttc cagaagaatt tatacgatcg
     1981 tgcgttatgt aagcccgaaa aaagtaaaaa tgccgtttgg aaaaagaatc gagcttttaa
     2041 aatatttccg cacagcagca gtatttcagc aaccgacaga taaaaagaaa acgaaagcat
     2101 ttaagaagca gtcgaagcca ggctggctga tctggtactt atttgataag aagcattact
     2161 ggctgttata ctttctggta acaggcaaac aaaggctgtc agagaaataa ggagcaggga
     2221 acatgaaaga aaagtcaatc aaattaaacg cagtgctcaa tgttatgaaa acggtattgt
     2281 ctctggtatt tccactgatc acatttccgt atatcacacg agtacttcag gtaaatgcga
     2341 ttggaaaata cgatttttcc gtatcggtta tcagctactt ttctctgatc gcagctcttg
     2401 gcatcacaac ttatgcagta agagaaggaa caaaataccg gggcgatcag gaaaaaatga
     2461 atcagttcgc aagcgagatt ttttctatca atctttatgc aacgatcgta tcgtatatct
     2521 tgttgttttt ggcacttatg tttgtagaca agctgaagag ctataatatc gtgatcggga
     2581 tcctcagcct ggaaattctg ctggcaacgc tgagcgttgt ctggatttat aatatatatg
     2641 aagattttgg ttatattacg tttgttacgt ttattcttca gttcgtatct atcatcctga
     2701 tgctgctgtt tgtacacagt gcagatgacc tttacaagta tgttattatt tccgttattt
     2761 caagcagcgg ttccggatta tttatgtttt tccactcaag gaaatatgtg cagctgcatc
     2821 tggtaaaaaa gccgccgctg aaacatttaa aaccgattat gatcgtgttt tcgacaacga
     2881 ttgcagctac catttatatc agctcagaca caacaattct tggatggctg acagatgatt
     2941 accacgttgg aatttacgga acagcggcca aaatatataa gattgtaaaa caggttctta
     3001 atgcagtcgt ggcagttgtt attccaagat tcgcatttta tattggaaca caggaaaaag
     3061 ataagatatt acagctggga agaaaactga tcgattatat ggtagcaatc tgctgtccgg
     3121 cgatggtggg actgttttgt ctgagtaagc caattgtaga agagtttgcc ggagcatctt
     3181 tcagcgaagc ctatatcccg ctgcagctgc tcagtattgc ccttgtcttt gctgtctttg
     3241 caaacttttt ttccaactgt gtgttgattt cttacaaaaa agaaaaaatt gttatgtttg
     3301 cgacaatcct cagtgcagtg ataaatattg tactgaattt tatcctgatt ccaagatatc
     3361 aggaaagtgc agcggctttt acgacgatta tcgcagaagt atgtgtatgt gtaatatccg
     3421 catacgagtc aagaaaaatc ataaagatca attgcacagc aagaaatctg atctcaacga
     3481 tcattggctg tgtggggatt gtgatctgct gtatgctggc tgcaaaagtt attgaaaatc
     3541 agatttatct gattcttgta tcggtgatcg tatcgggact ggtttatgca gttgttttga
     3601 ttctgatgaa gaatacagtt atcacagatg ttttggagcg ttttgtcaga ggagcaagag
     3661 gctaagggag aggaaaaatg cagggaaaaa gaaattcaac aatggacatt gtaaaggcga
     3721 taggtatcat ttctattgtg atgggacatt gctgctattc aatcatgata ccagccttga
     3781 acgtcagtgt gggagaattt gtatacagtt atcatttaat ggtgttcttt tttgtggcgg
     3841 gattttttta caaaagagaa taccatgagc acccggaaca gtatatcggc aagcggcttt
     3901 tgaagcttgg cggtatgctg tttctttata ataccgtgtt tacgctgtta cataacacac
     3961 tggtgtcggt caaaatgatc agcagcaccg aacattacag catcagcaaa atggtatcct
     4021 gcatcgtaca gagcctgctg atgaaataca ctgaagaatt gctgggagcc tgctggttcc
     4081 tgccaatgtt ttttatcgga acagcattgt ttgcgattgc attcagcaaa gcagaaaaaa
     4141 gtaaaaaacc ggaatattgg cataagatca tctgtattct gtttgctgct gttgcattat
     4201 atgcaaacag tcgggagctg acatgggaat actggataca gacttcaatt ctggcggttc
     4261 cgattatgta cattggttac tttgcaaaac agaaatggga caaactcgac aaagggatta
     4321 cctggtatgg aactatttta agtgctgccg tgattcttgg aatcttaaat cgtatgccgg
     4381 gaagtattga actttcggtc aatcagatct tacatccggt acttttttat ccggtgacct
     4441 tgcttggtat ttatttttgt attgggcttg caaaaatact tggcaaaaat ccatatacag
     4501 aaaaattttt ctcactggtc ggaaaagaaa gctttcacat tatggcactg cattttcttg
     4561 gatttaagat cgtggatcgt atttattcgt cagtgtatgg cattacagat gctgaaaaaa
     4621 ttggaaaatt tccacattca gattacggtc tgcacatcag ttatgtgatt gccggagtgc
     4681 tcattccatt atgtctgatt acattactgc gtaaagttca aaaatatggt cattttgtga
     4741 aagaaatgta aaacatgctt ttcaaacaaa acagaggaga tttttacatt gacttttaca
     4801 tttagataaa tatactataa attaggaata aaaatcgtgc tgcttggtaa aagtaaccag
     4861 agcagcatcg cagtgctaaa ttgcattgca gcatgaggag gaaataaatg aaaaaaagaa
     4921 tgcataaact ttgcaccatt ttgacagcat ccgctttact tgcaatgtct gtcacagtat
     4981 atgcagcaga aggggataat acagaggctg gatttcagcc tgcagaagaa gggactgtac
     5041 agtctgcgga taccgagcag acagaagaga aagatccgga agaaaacgga ttagattctt
     5101 ttgtttatat aagagaaaaa gaagataaaa caaccggaaa agcaaaagta aaaaaagtaa
     5161 cgatcgaaga attaaaagaa gagatcgatg caaatcagat gccggaactg gcagcggttg
     5221 tttttcaatt ttcagatgaa gagtttgaga cagaggcact gacggagaat acaactgaat
     5281 ttgaaacaga aactcagtca gaaacgacag aatctgaaag tgaaacagca acggaaacag
     5341 aaacagaatc cgagacagaa tccgagacag acaaagattc agaaatcgag aaatatccaa
     5401 ccgtagaact gagtgatatg cagaaagata tggtctatga ggtagcagag cttgttccac
     5461 agacggttgt gatcttcaac cagtgtgagc tggaaaataa tctggtgttt acagactgca
     5521 aggaagtgaa ggcagttacc gttctgaaag cagacacaga gaaagaactg aaaaaagcaa
     5581 tccagaaata cgacctggaa aaagaatatg cagattatct taaactccag agtgaaaaga
     5641 aatctgcgtc aaaaggacag gcacgtacat caagttcagg caaaagcctt gcagtgccac
     5701 agacgtccac tcagacagag agtcagactt ccactacacc gtcaaaccgt gtggcaggaa
     5761 gcagttcgac atcgggaagc agttcttctt caggaagctc tgcttatgga ttatccggca
     5821 gctccacatc aggaagcagt tcgacatccg gaacaacaac cggacagaag gacgttacat
     5881 tcaaatttga gccggatgag gtatacagcg gagaagagag catttcagca acttacagcg
     5941 tatccagcac aaaagagatt acatatggaa aagtaacgat taactatgat accaatgcga
     6001 tgaaatacga tgattccaat gcggaggaca gcgacgcact ggaaggtatg acgatcaaag
     6061 ttatcaagcc gacagacagt gccggaacgg aaggtaagat tgtgattgaa ttttcatcaa
     6121 caacaccgaa aaagctgaaa ggaactctgg tagatctctg gttcaatctg acaggcaatg
     6181 caacaaccgg tcagcagttc aatatcagca tgaccgtaga tgaattacga aatggatcga
     6241 ccaatctgac atctgaagtg gagacagtaa gtatggtagc acagccggat ggcgatgaag
     6301 agacggaaac agagacacct tctacaaccg ccacaccgtc cacaacggca tcgacaagtg
     6361 catcgtcttc cacaccgaca tcaggttcga gttccacgat gacaacacag aacgcaccaa
     6421 agacaggcga tacaaccaat atcccggcta tggttcttct gatggcaggt tcggcagcag
     6481 tatatatcaa agcaagaaag agagtatagc aataaatgta tagacgaaac atagcagcgg
     6541 cagcagccgt cctgtttctt tcttcagccg ttggcatttc tgcttatgca gcaccacaaa
     6601 aagacaacag tgcgacagag acagagactg aaacggaaac agagacacag acggaaacgg
     6661 aaacagaaac cgaaaccgag acggaaactc aggaaaccga agcagcgaca gagcgagaag
     6721 caggttttgt atccatagaa gagatgccgt ttgaaattga aacggaatct gagacagaaa
     6781 gcagcaatca gactgagacg gaaacggaaa cagaaacaga gcagaaaata gaatcaccaa
     6841 aagtcgaaca gacttttcga ttctttcagg tagaaaaaaa atatgccgta tcaaaaagag
     6901 atgatgttta tctctatgaa aagaaaaata caaaatccga taaagtagga aagatcaatg
     6961 cacttggcgt attgcatatt ctggaagaag acgacgatgg cgaatggtat tatgtagaat
     7021 ccggaaaagc cagaggtttc ataaaagcaa aatatgtatg ggcagacgaa aaagcggaac
     7081 gttatgtaaa gcataaaaaa gaaaagaata tagagacagc cgaagttctc aaagaagctt
     7141 atgaaaataa ggcgtttctg catacacaga caactgcata caaagcagta gccaaaaaga
     7201 aatacgccct gacgaaaaag agaatcaaaa tctatgattc tattccgcag gaagaaacca
     7261 agaacaataa aaaagaagaa acagaaaaag aaacgcagga aagtactgcc ttagaaagtg
     7321 aaacacagag cagcagtgtg acagagggcg aaacgcagga taattctgcg aaagagagcg
     7381 aaacgccgga cagtgcttcc agggaaagtg aaacacagga atccgagaaa aagaaaagca
     7441 cagatcagaa atcagagaag agtgaagata cagaaaagaa ccaggagccg gaggtagtag
     7501 gtacactggc aaaaggcgga ctgtgttata ttctggatga tgaggatcag gaatggtgtt
     7561 atgtagaatc agatgatgtc aggggatttg tacagacaaa gcagctcgaa accggaaaag
     7621 aagtgaaaaa agagattgaa gaaaaaggcg aggatactta tgcccttgct aaagcaaaag
     7681 taaaaccgga agacaacaaa gcctgctatt acacggtaac ttccgtaaaa gaagcttctg
     7741 tatcaggact gatccgcacc tctatgttag aatatgcgaa gcagttcctg ggtgatcctt
     7801 atgtatgggg aggtaccagc ctgacaaaag gggcagactg ctccggtttt gtacagagca
     7861 tttatgcaga atttggttac agcattccgc gtgtggcgga agaccaggca gaatgtgcca
     7921 ccaaaatacc ggtagaagat gcattgccgg gcgatctgat cttttatcag agatcggatg
     7981 gctatattta tcatgtagtt atgagtactg gtgacggcgg aacaatcgag gcacacagca
     8041 gtgccacagg cattatagaa agcactgtaa atgaaaacga tgcagtatgg gcagtccgta
     8101 ttatttcaaa tgaggataca gacatactgg atgcattgaa ggaaaaggat atggcagcag
     8161 attactatga taatgctgtt atagcgaaga gcacagaata tggttcatac cttggcaaat
     8221 ttaagctgac agcatattgc agctgtccga tctgttgtgg agtctggtca ggcggaccta
     8281 cggcgagcgg agcaatgccg acgatcgatc acactgtagc tatggcagga ctgccatttg
     8341 gaaccgaatt gatcatcaat ggacaggtct atacagtaga agatctggga acgccatatg
     8401 gacatgtaga tatctatatg aataaccatc aggcggcact gcagtttggt gttcagtaca
     8461 gtgatgtgta tctgaaaaaa taaagaagaa taaatatttg atatgggcaa tttcttatta
     8521 tattcaagaa ggaattgtcc atatttttat atcagatgtg agatgacttc ggcagtcgct
     8581 tctctgcgtt caaatgagga tacagacata ctggatgcat ggaagaagaa taggtgagtt
     8641 gaaaaaagta aatttcactt tgcaagtgag ccctgaaaaa ataaataaag aaaaagcaaa
     8701 aaatcagttg acatttcaaa atgaatttat tataataaat gagcgtcaca gagatgtgag
     8761 cacaatatca gacgaattgc tgattcagga gaagtactca agtggctgaa gaggcgcccc
     8821 tgctaagggt gtagaccgtt tacgcggtgc gagggttcaa atccctcctt ctccgttcaa
     8881 cattctaaga tattttttta tgcccttaat tctttgaaaa acaacttgag aaaaataaat
     8941 caaaaaagtt gttgacaaaa agaaagagaa gtggtaagat atcaaacgtt gcggcggaaa
     9001 cgcagaaaca acggagcctg aaagatacag gcagtcaggg aatcatccag acgatccggg
     9061 gacggaatca aaaagatgaa aaaagtactt gacaaaccgg aaagactttg ata
//
LOCUS       DAWDWH010000073         9017 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3824_length_9017_cov_10.802388, whole genome shotgun sequence.
ACCESSION   DAWDWH010000073 DAWDWH010000000
VERSION     DAWDWH010000073.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 9017)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9017)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9017
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3824_length_9017_cov_10.802388"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1398..1491
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2041 a         2284 c         1770 g         2828 t
ORIGIN      
        1 gttgtcgtgt agattccttt tactttctgc catttttccc ggattttctt tgaaagtaaa
       61 acatcctcaa aaaagatatt gatcttttct gcttttttca ggatttcact ctggtcttct
      121 aatcttttca catacgcgtg gtttgaaagc atatattctg caaaattccc ttcgatttct
      181 gccgtaagga aatattctct gtcgagactg tccgcataga cttttccatc tgagataact
      241 tctgcataat aacgtgattc tttccctgct ttttcctgat gcacccggta catttcccgc
      301 acatccttta agatctgttc tgccgatcct tccggaagac attttttata taatgcacat
      361 gtctttttcc agtcccagac aacggatccg tttccaagaa ccgcatacga aattcccgaa
      421 agatttttga ttttttccgg aagaagcggc aatgcccttc ccgtagcggg aaggacatgg
      481 tgcccctgat ccagacattt ttgtagaaca gcacaggttc gctctgagat ctgtcccctg
      541 ctgttcaaaa gtgtgccgtc catatcgacg gcgatccaat atctgtctgt catagatttt
      601 gttccttttc cgcctgtaat ctaagatgaa atcgtttcgt ttttcgtata tcttatgttt
      661 tcatctcttt acaagctgtg tttcactgaa ttttcatcat aaatgcttcg tatgggcgaa
      721 gtgcttctgt taaatctttt cgctctgcat agtttgcgat cttgacttcc tgcactcctt
      781 tcgcatagga cttcaaaatt tctgccggaa tcgtctcctc tgagagattt gctgccacca
      841 gccaacattc atctgctgtt ttgcgaagat aggcaaaaat gtgttcctct tcagcaagga
      901 tcagctcgta gctgccttct gtcagaagtg gttcctgatg acgcatctcg atcagttttt
      961 tataataata gaaaatggag tctttgtctg caagtgcttc tttgacattg atttccctgt
     1021 aacggtcaga cagtgcaaac catggctttc cggttgtaaa tcctgcattt tcactgtcat
     1081 cccactgcat cggggttctg gcattgtcac ggctcttgtt ccagactgcc tttttgaact
     1141 cttcttcgga aatgcttttt ccattttcca ccagttcttt gtatgcattt cgcacttcga
     1201 tatcttcata ctcatcaagc ggaaaatgga catttgtcat accgatttcc tcaccctggt
     1261 acacatacgg tgttccctgc attccatgaa gaacggttgc atacgctttc gcacattccc
     1321 tgcggtaagt ggtatcattt ccccagcggg aaataatcct cggctgatca tgattttcaa
     1381 aatacagagc attccatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggccgctga
     1501 gtttgtcctg ccagttggac atgacacgtt tcagttctct caggtcaaat ggttttaatg
     1561 cccacttgcg gttgaccgat cccggaatgc ggtcgatgtt catatgctca aatgtaaaga
     1621 tcatattcag ctcttttcgc ttcggatcgg tgaaaagtct tgccacttcc ggtgtggaac
     1681 caggtgcctc accgacagtc atgcagttgt attttgagag gacttcctga ttcatctcct
     1741 ggataaattc atgaagtctt ggcccgttgc tgtgatattt tccggtataa tactttcttc
     1801 ccggctctgt ttcttcgtag tccgggaaat tctggtcttt tgaaatggaa gcgatcacgt
     1861 ccatacgcca gccgtctgcc cctttttcca tccagaattt catcagatca taaacttcct
     1921 ttcggacagt ctcgttttcc cagttcagat ccggctgttt tttgctgtag aaatgcaggt
     1981 aatactgtcc gcgttcttca cagtactccc aggcacttcc gcagaaactc gaaccccagt
     2041 tgttcggctc gctgccgtct gcttttggat ctttccatat gtaataatcg ctgtatttgt
     2101 tatcttttga cttgcggctc tccacaaacc aggcatgttc gtccgaagta tggtttacca
     2161 ccagatccaa cacaactttg atttttcttt cgtgtgcctg tcggatcagc tcttccatat
     2221 cttcgtttgt tccgaatttt tcgtagatgc tgcgataatc acggatatcg tatccgttgt
     2281 catcctgtgg agaatcaaat accgggctta accagatcac atcgattccc agttctttca
     2341 gataatccag cttttcgatg actccgcgaa gatcgccgaa gccatctccg ttcgagtcct
     2401 taaagcttct tggataaatc tgatatacaa cagaattttt ccaccagtct ctcatttttc
     2461 tttactcctt ctctttccct tatgcaattc caagtaacga caatacaatg ccaagaacga
     2521 tgacaccgat gatcagtacg gtgatgctga cgttcttctt tttcagaagg taatagcaca
     2581 aaagagtcag tcccagcgga acgattccaa tgaaaatctg atccagcata gactgcagat
     2641 tcagaatgga ttctccttgc atactcagtt caaaagtaga attaaaagtt accatgctcg
     2701 ctgtcatacc gccaaccatg atcagaccta cggtacttgc agcttttgta agaatattca
     2761 tcagaccgct tgcataaact ttctggatat attcggaacc cagtttgtat cccagaaagg
     2821 ttccataata tttcacaaga agggacggaa tgttaaacag cagtaagaat acgatcggtg
     2881 ccagaatatt tccggtcata ccaagtccaa ctgcgatacc ggctgcgatc acgcgaagaa
     2941 cgccccagaa aatggaatct ccgatacctg ccagtggtcc catcagactg gattttactg
     3001 cgttgatgga atttgcatca aaatctttct gctcgctgtt ctttttctcc atggaagcaa
     3061 ccagccccat gataaaggtc gagcaggtga tcgtggtatt gaagtaagac atacttctta
     3121 ccagtgcttc ttttttgcct tcttcatcat ttttgtagaa tttattgata aacggcatca
     3181 gtgcgtaaca gaagccggac gcaccctgtt ttgccgggct tacggctgca tataaggtaa
     3241 aggaacgcca gaatgccttt tttaacattt tccgttcatc tgccggaatg gattttgtca
     3301 gattactcat gagaaaaatt cctcctcttc ttcatcaaat tcactggact ggttacctgc
     3361 cggtgcagcc acacgctgca tagacagctg attgatcttg tagtcgttca tgccaatcga
     3421 gatggcaacg atcacaccga tcactgcgat tgcgatcagc ggaaggttca tgtatgcaga
     3481 aaggacaaat ccaaggaaat agaacataca gattttcttg tcccacagca ttttcatcag
     3541 cattgccatg ccgactgccg gaagaagccc gccgcagaca gtcagtccgt tcataaccac
     3601 atccgggatt gcatccatca gagtactgac tgcatcggaa ccgaacagga ttccaaggaa
     3661 tgcaaccagg gaatacagta tccagttgac cgcccacagt ccatagtgca gaaaaacgat
     3721 ctgtttttct tttccttctg ctgccagctg gtcaaatttc tttgcaaaca taccaaccag
     3781 aaagatgtac aggaatgttt tgatctgaag tcccaaaata ccgatcggca aagccagtgt
     3841 cagggcaact tcacttccct tacccagcat gattgcaaat gccgtaccga ctgctgttgc
     3901 aattccaggt tctgccgctg aagcaccgcc gatatttaca actcccataa acacggcttc
     3961 cagtgaagca ccgatcataa cgccggtttt tacatctccc aggaacagac caaccaaagg
     4021 tccgatgacg atcggtcggt ttaacatcgt aaaaccaatc aattctgcac cgccgacaca
     4081 gataaatacg gccagtgcca ctaaaaatgc atttaacata atctctttct ccttttcaat
     4141 gattttagga tcaaacgtca tcaactacga tacctacgga ttgttccgtg ctttgcactc
     4201 tcacaatccc ttggtatctt ttaacttgtc cagcacatct ttgacaagtt tctgctcatc
     4261 attcggaacc tgctgcatgc aggtctttgg atcgattgcc accagttctc ttaagttctg
     4321 cacttcttcg tctgtcagca tgatggtcgg ggaaagcagc tgtttttcag atacatcgat
     4381 tccgtcgaat cttccggtgt ttccgatgtt cagaaattca atctcaccta cctgtctgcg
     4441 gatcttcagt gcatccttga cggttcttgt cagaacgagg atcctcattt ttgatgcacg
     4501 cggatcattc agaacacgga tggcttcatc taccggtttg atcagtgcct tgatgcctcc
     4561 cggaactgcc attttcagtg ccattttctg cgtatcatcg tttgctgcat catcatttgc
     4621 caccacgatc ccctgtacac gcaactgttt ggtccaggtc atcgccacct gtccgtggat
     4681 cagacggtca tctacacgca gtgcggaaat ctttgctgtt gaattttcat agtttgcagg
     4741 ctgtgcggct tttttctcct gaaccgcagc cgctttcttc tgactctgtg ccgctgcatc
     4801 tgcttccttt ttcggttttg catgcatctg caccatctga agttctcctc ttgcttcctc
     4861 aatgtaagtt tcgatctctt cttcgctcac atcatcatcc gcaagcatga cctgcatcag
     4921 taccgggaga tttgttccgg tcaccagtgt gacgttgtcc tgctttgcgt actccatcag
     4981 cttctggttt acgcttcctc ccatcaggtc ggtaaacacg atgatgcggt cttctttatg
     5041 cttctcaaaa aattctttaa actgctcttc cacattgtct tccggtgtta cataagcatc
     5101 aatcgtcgtg atctctgcca gctgaccgac gatcagctcg gccgtgtaac ggattccgga
     5161 ggccattttt ccatgtgtgg caatcaaaat ctgtttcatg tgatcctcct ttgaagtgtc
     5221 tgttctgcgt catatttcgt taggttatgt acataacttc tatgtacaca ctatagcatt
     5281 tgtcgttttg tctgtaaata ggttatgtag ataaccgtct ttttgtataa gttcagatgg
     5341 gggatttttg tgcattttga tagattatga aaggatttat tgaaaaaggg aaggttcgta
     5401 taataaaaac gccctgattt tggaaaacaa atgctctgac acgctgcgtt tcctgttcgg
     5461 tgtcctctgg caagccagga acctttgtca atgtgcaaaa tgaacaattc cccagtaggt
     5521 actatctgat gtaccgctgc caatgtgagg tagcgagagc gtgccttcgg ggaattcgtt
     5581 catttgcaca ttttcatttt ttttgtgtgg atgtcatttt tctttatcac gcaaaatcaa
     5641 acaatattgc tgaatcttgc ttttcagtct ctctccaccg acttcctttt caccagtgtc
     5701 acggacagtt ctacttcccg ctgcggatat tcttcgcctt cttcgatcca tttcagaagg
     5761 atttccactg ctttctgtgc ttttttctgc acgtcctgct ttatggtggt aagtctcggt
     5821 gtggagtact gtgcatagct gatatcgtca tagcctgcca cgccgatctg ctgcggcacg
     5881 gctacttttt tgtcggcaca gaagcggatc gcttccagtg catacatatc ggagaggaaa
     5941 aacagtgccg ttcccggcaa tcttctctgt accagccagt cataacttgc ttttcgtttt
     6001 tcatagttca tgccaagtgc cacaaactgc agtttctggc cttctgccgc acactcctgc
     6061 atggctctct gtgcaccgac ataacgcaga tgatcgacac cgttgtctct tccggcacag
     6121 actttgatgt gctcatatcc gcattccagc agatatttca ccatctgata gccgccactc
     6181 tcgtcatcca gaccgatatt gaccagatgg ctgtctttgg ctgcgtttcc gtaggcatcg
     6241 atcgacacga tcggtttttt gatcagctga tagatttttt cacagttgtg tcggctgaag
     6301 gagatggcga tcacgccgtc cacatcccag cctgccacca tctgaaaaat ctcttcgatg
     6361 tcttttgtgg aatacagcat caggtaatag ccctgtttct gtacatattc ttcgataaat
     6421 ccgatgattt tcccgtaaaa tggatctccg agaatggaat ctttgaattc tttatgatag
     6481 ttgatgacaa ctgcgatcag ctgagacttc tcgttattca gcactcgcag tcctcttttc
     6541 tgcacgtaac caacttcttt gatcagtgct tcgatcttct ggatcgttgc aggtgatact
     6601 ttttttgttt tcccgtgaat gacgttggaa acggttgtgg tactgacacc tgccttttct
     6661 gctatttctt tgattgttgc caaaaatatt ctcctaatta ttcgagcccc cgcagaacct
     6721 tgcgtaccgc agcataggaa aatccttttc tcataaagaa cacatagtat ttctgctgtt
     6781 cttccgctgt tggattctcc aggttcatgc gtttcttttt cgcaagtgcg aggatcgttt
     6841 cttcttcgct gtttccttct gcctgttcgt atgccgcttc aatatcttct gaagaaacgc
     6901 cttttgcttt taagtcctgc acgatctggc gtttgctctt tgtctggctg cggctctcga
     6961 tatagtatcc ggcataacgt acatcatcga tatagtggaa gcttcgcaca tactcgatag
     7021 cgatatcttc gatctctttc gggtatcctc cctgacggag ctttgctctc agctgccatt
     7081 cggtcttatc tgcacttttt aagatattca tgcaacgcag cctggcccgt ttgggcagga
     7141 tctcctgcat gatctctgca tatgcctgct ctgaaaggga ttccccttca cggatcccca
     7201 tccggtaagc ctcattccgg tacaggacaa aagctttttc tccgtctaaa aagacctgca
     7261 ctctcgtttt gccgaccggc tctaatgcag tgacgatcat gcttcatccc cgctgccgtc
     7321 cggatctgct gccggagttt cttcttttac ggcatccttt ttcgcagtac tgctctttgt
     7381 tgcttttttg tcttcttttt catcatctgc ggctgccgca ccgtcaaatc catacttttc
     7441 acgcaccagt ttttcgatct ctgccatcgt ctccggattg ttcttcaggt aaatcttggc
     7501 attctcacgg ccctgtccga tcttgatgcc gttataggca aaccatgccc cgctcttgtt
     7561 tacaatctca tttttcactg ccagatcaag gatgtcacct tctcttgaga tgccctgacc
     7621 gaacatgata tcaaactctg cctctttaaa tggcggtgcg atcttgttct tgacgatctt
     7681 gacacgcaca cggttgccga cgatctcgcc gccctgtttg agcgtctcga tacggcgtac
     7741 atccatacgg atggaagagt aaaacttcag tgcacggcca ccggttgtaa cctccggatt
     7801 gccgaacatg acaccgactt tttcacgcag ctggttgata aagatcacgg tacagctgga
     7861 tttgccgatg ataccggtca gtttacgaag tgcctgagac atcagtcttg catgcagacc
     7921 tacgtgagaa tctcccatat cgccctcgat ctctgttttt ggcaccagtg ctgccacaga
     7981 gtccacgatg ataatgtcca ctgctccgga acgcaccatg gtctctgcga tctccagtcc
     8041 ctgctcaccg ttatccggct gtgaaatgta aagattatca atgtctacgc cgatattctt
     8101 tgcatagacc gggtccagtg catgctctgc atcgataaat cctgcgatac cgccgcgttt
     8161 ctggacttct gctaccatat gcagtgctac ggtcgtctta ccactggact ccggtccgta
     8221 gatctccacg atccttcctt ttggaacacc gccaagtccc agtgccacat ccaggctcag
     8281 agagccggtc ggaacggtct ccacgttcat atggtttcca gaatcaccca gtttcataat
     8341 ggcacctttt ccaaaatctt tctcaatttt tgtaatagcc gcttccagtg ccttgatctt
     8401 atcatcctgt cccattttaa tatcctcctt aatcttctat ttgctctata ttgttccact
     8461 tgtcttcccg tatcaggtaa atatctgcaa aacggacccg aatcgcctgc caggtctcac
     8521 atgtgttcga cttgaaaata ctcaactgta ctgaccacaa gcgtatactt tcgatacaga
     8581 ttataccaat ctgatacctg gtttctgtaa tgccaatggt tttctcattt cttctgtgca
     8641 cctttgcaat acgcaaaagg gcttgcttgc gaacttacgt tcgttctctc cccatattaa
     8701 tatatatttt taaaaaagtc aacctaaaag cgtgtccatg tttggaaatt tgcagcagaa
     8761 gtgctgcctg ctgtttatcg aattcaagaa tgccagagtc ggaattcacc tcacatctga
     8821 tgtcattctt ctctgagtga aatgaaatgt caaaaggaag gacaaaatta tccttccttt
     8881 attatagttt actctttact tattgtcaat ttatttctcg ttctgttttc tcttttctga
     8941 tgttcgtatc aaaaaatgtt gttcgttttg ctccgatcga agcggagcgt agaaaggatt
     9001 gtgggagtgc aacgcac
//
LOCUS       DAWDWH010000074         8855 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3899_length_8855_cov_11.872841, whole genome shotgun sequence.
ACCESSION   DAWDWH010000074 DAWDWH010000000
VERSION     DAWDWH010000074.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8855)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3899_length_8855_cov_11.872841"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2879 a         1781 c         2232 g         1963 t
ORIGIN      
        1 gcggcggtat tgcctatgtt ctggatgaaa acaacgatct gtatacaaaa gtcaacaagc
       61 agatggtttc cgttgaaaaa cttggcaata aatacgatgt gcaggaatta aaagatatga
      121 tcgcagagca tacggcatac accaattctg caaaaggaaa agaaatcctg aatcattttg
      181 aagaatactt gccgaaattc aagaagatca tcccgcatga ttataaccgt atgatgatgg
      241 caatcgtgca gatggaagaa aaaggtctga gcagtgagca ggcacagatt gaagcatttt
      301 atgcaaataa agcgagatag gagggaaaag aaatgggaaa accaaccgga tttttagagt
      361 acgaaagaag agtgagtgaa gagatcgcac cgcttgaacg tattaaaaat tttaacgaat
      421 ttcatatccc tctgccgctg gaaaaacagc agcttcaggg agcacgctgt atggcatgcg
      481 gagtgccgtt ctgccagtac ggaaaaatga taaacggcat ggcatccggc tgtccgctga
      541 acaacctctg cccggaatgg aatgatcttg tctataccgg aaactgggaa caggcttaca
      601 accgtctgat gaaaacacat tgttttccag aatttacttc cagagtgtgc ccggcactct
      661 gtgaagcagc ctgcacctgc aacttaaacg gagacccggt ctccacaaaa gagaacgagc
      721 gtgcgattat tgagcgtgcc tacgagacag gacttgtcaa ggcacagccg ccgaaagtgc
      781 gtaccggaaa aaaagtagcc gtgatcggaa gcggtccgtc cggacttgcc gttgccatgc
      841 agctgaaccg ccgtggtcat gaggtgactg tatttgagcg aaatgaccgc atcggcggcc
      901 tgctccgcta cggaatcccg aacatgaaac tggagaaatc tgtgatcgac cgccgtgtcc
      961 gtctgatgga agaagaaggt gtgatcttta aaaccaatgt caatgtcggt gtggatgtca
     1021 aagcagccaa acttctgaaa gatttcgacc gtgtggtact ctgctgcgga gcatcaaacc
     1081 caagagatat caaagtgccg ggacgagagg cagaaggcat ttattttgcc gttgatttcc
     1141 tgaaaggtgt gacaaagagc ctgctggatt ctgatttggc ggatcagaaa tttgttgcct
     1201 gcaagaataa gaaagtgatg gtcatcggag gcggtgatac cggaaacgac tgtgtcggaa
     1261 cagccatccg cctgggtgca tcttctgtgg tacagctgga gatgatgccg aaagccccgg
     1321 accagagagc ggcaaacaat ccatggccgg agtggccgag agtctgcaag actgattatg
     1381 gtcagcagga agcgattgct gtattcggac acgatccgcg tatctatcag accaccgtaa
     1441 aagaattcat tgccgatgac aaaggcagac tgaaaaaagc agtgctggtc aaattacaga
     1501 gcaaaaaaga tgagaaaaca ggtcgcatga tgatggttcc ggtggaagga agcgaatgga
     1561 cagttgaagt ggatgtcgta ctgatcgcag ccggttttct tggctcacag aaatatgtga
     1621 ccgatgcctt taaagtagat ctgacaccgc gtacgaacgt ggcaacggaa ccggaaaaat
     1681 atcagacttc cgtaaagaat gtctttacgg caggggatat gcaccgcgga cagtctctcg
     1741 ttgtgtgggc gatccacgaa ggaagggaag cggcaaaagc ggtagatgaa tccctgatgg
     1801 gatatacgaa cctcgtttaa agaaaacgaa cgcaaaaagt ggggcagatc attctgcccc
     1861 aagttccagc atcacactgg cgatatacca tccgtctttg atttctctgg tggagatccc
     1921 gttgtaacgg cggacgatat catagatgat ctgacggcca agcccatgct gctttttatc
     1981 cgccttggtc gtgcggatgg taagtgcctt gtcttgcatg gaaggatcca ttttgtttcg
     2041 aatggtcagg gagagataat ccttgaccat tttcatttca atcacaattt tcggatcttc
     2101 aatttttttg gatgcttcga tcgcattgtc cagaagattc gcaacaagag aacagagatg
     2161 gtgatcgggg atcgacagat gtggaggcag tacgatctga atatccatcg ggatctgata
     2221 cttatgagcc gtggcgtatt taaggttgat cacctggttg acaatctcat ttccggtctc
     2281 aatctgattg tcgagtgcat aaatctcttt ggaaagattg taaaaatatt ctttcagggc
     2341 atcgtatttg ttctggctga gcagacgatc catgcaggca aaatgatttt tcgtgtcatg
     2401 ccgcaggtgg tggtattctg tcacaatcgt attcagatct ttcatatgct gcttttgata
     2461 atcattctgc tggttgatca gctgaagctg gatgcggctg ctgtattcgc tggtcatctg
     2521 ccataccata taatatagaa gcaactcgct caccagagag aaggttccga tgtaattgat
     2581 cacgccgctg acagcatcaa aataattttt cagcatcatg acggtgcagg aatttaaaat
     2641 cggagtcagg atcatgacca tataatagga aaacggataa ttttgttttg tattcggtcg
     2701 gaaacggata aaaaagcatc cgcacaaaac aagcagaatg cttggcagaa gagatttatt
     2761 ataagaatga agcgtaaata taatactgtc cggcagaata tgggaaagca gattcaacag
     2821 gatgctgaac ggaaagcggc ataagagcag aattgaaata tagatcaaag gcagaaagaa
     2881 tttaaagctg gcccttccct cgcatatcac aaaagcaaat aatattaaaa aaatggcatg
     2941 taaaagtata tagagccaga agacatcgcc atacaacttt ggcagataat attcttcaat
     3001 cagaagaaaa atcgcatgga cgcaaaaaat gccggttatg cagggaatcc tcagacgaaa
     3061 tgtctttggt gtgaggattt tacagagcat cagctgccct aaaaaaactt caagtaaaag
     3121 agagaagatc ttgatgatcg ttccaaggga aaattcaggc atcataacgt cagcctccga
     3181 aattctactt tgatgtcatc cagacgatag cggctgaccg ggagttgctt tttgctgtcc
     3241 agaacgacac aggttgtctg aatgttttta atataacggt aattgaccag gtagcttttg
     3301 tggatacgga taaaaccgta aggatgaagc tgcttctcca tgtcagacat tttaaaacgg
     3361 attgcttcac tttgctgatc cccgctaaga aaaagcgtca catatttatc gatactctgc
     3421 acgtaaataa tatcatcaat ccggaaacgg tacatcgaag tacggttctc aaatgtcaca
     3481 taatcctgtt cggactctgc atggcagtcc gcaaggatat ctgcgataca gccggaaatc
     3541 tctgactgaa actcgtcctt tgggataaaa cggaatggtc ttgccttgaa ggattcataa
     3601 acacattcgc ggtggatgga aacatagatc agataacagt ccatatccag ctcacgcagt
     3661 ttctgtccga gactcagtcc atcaatcagc ggcatatcga tatcaagaaa acagatatcc
     3721 cagcgtttcc cttcatataa agaagagagg agttttccgc tgtccgtaaa aagctcaatc
     3781 tcacattccg tcgaatattc cttaaaaatc cgctctatct gttcataata atattcagca
     3841 gtaatgtatt catcatcaca aatcgcaatc ttcattttat cacttcctgt aatctaattt
     3901 gaacgcagag aaacattcca ccgcttcaag tgtgcagagc actaagcggt gggtgaatgt
     3961 ctgctttaca tatatacgga acagaaatca tgcttctgtt ccgcctggta attttcatta
     4021 tagtggatta tggatagaat gtcaaatttc ataggaagac tgaatgaaaa tttgacattt
     4081 acgacattgc tttgccattc acgcctatat ttgctataca cgacacacag attgaaactg
     4141 aaaaaagagt gtgatagggt aaaaattaca agaggcacaa aaaaggaaaa gcaaggagga
     4201 ataatatgaa acgaaacaga aaacgccagg tgacggctat ggcaatggca gtcatgatgg
     4261 cagcagcaag ttttaatgta agcggaatcg caatgccggt gtatgcggca gaacaaagtg
     4321 acgagatcca tttcccggat gtctatgcaa aagacctggt tcagccgatc aaagatcagg
     4381 aaaccatcaa taagaaagtg aatgctcttc tgaaaacgat gacaacggaa gaaaaataca
     4441 ccttcctcgg cggaaacgga accggtgata cggaaggaaa tgccggatat ctgaaaggtg
     4501 tgccaagact aggcgtgcca atgatcaaga tgtacgacgg acctgccggt cttctctatc
     4561 cgcaggatac cacaaacccg ccgcaggagc agatgcttgc agcgacctgg gatgagtcca
     4621 tggcagagat gtacggagaa gctgtcggaa atgaaaacct ggcaatcggc ggatgtatga
     4681 tgttgagtgc acagcttgat attcagagac agccgcagtt ccagagaacg aaagaccaga
     4741 tgggcgaaga tccatacctg ttatccagca tggcagatga tctggtaaac ggcatgacca
     4801 agaatggcgg tatcgcagtc ctgaaacatt atggagcttt tgcacagaat gcatcaccgg
     4861 gaacaaacac aaacgtagaa gtttccgagc aggcactgca tgaaacctat ctggcaggat
     4921 ttgaatcagc aatcaaaaca aatgattcga tcggtgtgat gtcatcttat aataaactgt
     4981 ccggcacatg ggcttctgca agtacggaac ttcaggaaaa tatcctgcat aatatgtggg
     5041 gatttgatgg gtttaccatc acagactggg gtggaaacca cgagtataca ctggataaag
     5101 gaacggacgt tgaaatgccg tccttaagaa acaacagcca ggcaaaaaca caggcgaaag
     5161 tggatgccgg tgagatgaca caggaagagg cagatgcact ggttgacaag agcgtaaaac
     5221 gtgtactgac tgcttatgcc aaaggcggat atctgacgct ggtacaggtt gatgaagacg
     5281 gttatgcaaa agaagaagca ggcagaacgg agctgatcaa gccgggtacc gataaccagg
     5341 cactggcaga tctgtatgat gcaagcaatg aagccgttga gaaagttgca gaagaaggcg
     5401 gcgtacttct gaaaaatgaa aacaagacgc tgccacttga tacaacaggt gacaagagcg
     5461 tggcagttgt cggcgtgaac ggtatgaccc tgatctctgg tatcggtggt gaacgttctt
     5521 acggttctat tcgtgcgatg acaagcccgt acgaagcact gaccgatatc cttggaaccg
     5581 ataaagtaga aggacaggtt tacaacgaca agatcggaac gatcatcccg accgaaaatc
     5641 tgtatacaac agcagccgga agcgagcatg gtgcagtccg tacctacggt accggaaaat
     5701 cagcagagac tggtggaaat tatcagggac agtttgtaag caacagcgtt ccggagacca
     5761 agatggaagg acacgaaatc ggagaattct gccaggttga cgaaaagatt gatttcaata
     5821 caaacagcaa aacttatcgc aatgcagaag gcggaaatgc ttttgattat acacagaacc
     5881 cggcctatac ctggacgact tatctggaag ctccggaaga cggtgaatac agcattatcc
     5941 tgcagagcat cggtgcaaaa gcagccatat ccatttatga actggatgaa gatggaaatc
     6001 cgacggaaaa agcgatcggc agcggttccg gtgcaagtac aaatcagggt acacagtact
     6061 atggaggcat tattccatct gaaacgggag aaaacctttc aactgcaagc gttaaactgg
     6121 aagccggaaa gagatatatg attggtattg cttccgaaaa tgcaggagca gtaaacggca
     6181 aagatatgca ggtcggactg gcatgggtaa caccatctca gaagcaagcg aacattgaca
     6241 atgcgatcaa agcggcagaa aataatgaca cggtagtggt ctttgcgtat gcacaggtaa
     6301 gtgatccgaa gagcacaaga gaagcaacca cactgaaact ggatgcttat cagcagacca
     6361 tgatccagaa tgtagcgaaa gcagctcatg aaaaaggcaa caaagtagcc gttgttctga
     6421 acaatgactc cgctgttgtt atggaagact ggattgatga cgtggatgca atccttgaaa
     6481 tgtactatcc gggacagcga ggcggtgttg caacagcaaa actgctgaca ggtgaagtga
     6541 acccgagcgg aaaactggct tacaccattc caaagaaaga cacagatacc ctgatcacca
     6601 tctcggatga agcatggaat cgttttgaaa aagaagatga aacagaagat actgaaaaac
     6661 agagcgatgt tgcaactcag gaagccggag acggattccc gggaggtgga ttcccaggag
     6721 gtggattccc aggaggcgga ttcccaggag gtggattccc gggcttcgga ggcggaaaag
     6781 ccaatacaac ctattttgat gagggaatca ataccggata taaatggttt gatgaaaata
     6841 atatcgaacc gcagtttgat ttcggatacg gactttccta taccacattc gaatatggcg
     6901 atatgagcgt tgcagaaaat gcaaccgacg gtgaagttgc cggctatgat gtaaccttta
     6961 ctgtaaccaa caccggtgat gtggccggaa gtgaagtcgc tcaggtatac cttggtgaag
     7021 cacaggtacc ggaaggtatt cagacatcaa aatatgcact ggcaggttat gagaaagtta
     7081 aagatattca gccgggtgag acaagagaag taacggttca tgtaagcgga cgttccctgt
     7141 cctactggaa cagcaatcag gaagaactga atgtaaatgc agacggcaca aaagataagt
     7201 ggacgctagc aaccggtgag agaaagattt atgtcggatc ttcttctgac aaactgctca
     7261 tggaaaagac cgttgatgtt gagaaacaga caaacagcat tgacatggta aaacagggac
     7321 tgtcacgcgt gatcgccctg atcgagagtc tcaacagttc tgaatatacc aaagaaagct
     7381 gggcagccgt agaagatgca cttgctgatg caaaagaagt acaggcagat aaagatgcaa
     7441 cggaaagcaa actcacagag gtacagaatg ctctgctgaa ggcatacaca gcacttgagt
     7501 acggcgtaca gaagacacat ctgcagatcc tcgttgattc tgcaaacgat atcctcaaag
     7561 aggcagcaaa ttatgaaaat aatgcaaaaa cactcaaaga tgccgtagca aaagcagaaa
     7621 aagtgctgac aaacgaagac gcaacgcagg aagaagcaga tgcagtcatg acagaactgg
     7681 tgaaagcact gcaggaactc tcagaaaaag caagcgtgaa gtccttaaaa gaactgatcg
     7741 atgcagcgaa agagatgatc gagagtagta actttacatc agcaagccag aagaaattag
     7801 aagatgccgt agcaaaagca gaagatgtgc ttacagacgg cgagcataca tccgcagagc
     7861 tggaaaaagc atacaatgat gtgattgatg cgatcattaa cctggaacgc aaaggtaaca
     7921 aggcagcact ctctgccatg atcgaaaaag cagaagaagt tttggcagat aaagatgcct
     7981 atgtagcttc cacgatcgac ggactggatg caatccttgc caatgcaaaa gcagtgaatg
     8041 aaaacgaaga cgcaacacag aacaccgtag acaacatggt gaagactctg acactgaaag
     8101 tggcagatgc acgccttaaa ggtgacgtgg atggtgacgg aagcgttggc acaagcgaca
     8161 gtgcaagcct tcttcagtat gcagcagaaa agatcactct ggatgatgta tcaacacaga
     8221 gtgcagatgt aaacggcgat ggtgctgcag atacacagga tgcggcaatg atccttcagt
     8281 acgcagcaga agagatcact tccttctaaa agcaggtttt tgaaataaaa cctgaaaaaa
     8341 tttccttctt tacacaaaaa aataaaaggc tgaaaaaacg gatttttcag gcgaaaaggg
     8401 cttcatgcta cacaggcatg gagccttttt ctgtgcttga cagacgtttt taaatatgtt
     8461 accaatataa aacatagaat agtttagact aacaaaagaa agctgaaacg tacaaaagtt
     8521 acaagtatac aaatagatat accggaaaag ctgtgcggtg tgaggctgac atggaggaga
     8581 ataaaatgag acgaagattt ctggcgtttg tgtgctgttt cggtatgctg gcatctttgc
     8641 tgccgtgcag tgtaaaggca aatgggagaa caacgaacgt aaatctgctg gaaacaccgg
     8701 aagaagtggc agaagtgttt tacaggcaga atgcatctct tagaaagagt aagggggtca
     8761 cggtacagga tcaacaaaat gagattcgca aacgtgtgat cgtagagagt gcacagactc
     8821 tggaaaatac atacggagca gtaaagacgt attac
//
LOCUS       DAWDWH010000075         8750 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_3937_length_8750_cov_12.467740, whole genome shotgun sequence.
ACCESSION   DAWDWH010000075 DAWDWH010000000
VERSION     DAWDWH010000075.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8750)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8750
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3937_length_8750_cov_12.467740"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    276..359
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7351..7360
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2039 a         2382 c         1797 g         2438 t
ORIGIN      
        1 gatcacgccg acaattctct tgacaacggc tttcaggtag tcctcgttgc ggtcgctgcg
       61 gtcgatgact ttctcccagt cccactgatc cacgtaacag gagtgaagat tatcaagctc
      121 ctcgtctctg cggatggcat tcatgtctgt cagaatcccc tttccaacac gaaaatcgta
      181 gcggtaaagt gccatacgtt tccattttgc cagagaatgt acaacctgtg cctccacgcc
      241 agctgccggg atttcaaaag aaaccggtcg ttccannnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
      361 agagcacgga atgatagttt tccggaataa ttacctgatt catctttctt acctcctgtt
      421 tgtggcaggc tcccgcaaga aaagcctgcc gctgtgttca tttttatttc tgcgactgat
      481 cgtttgtcag tccgaattta tcaagaatat aaaataccaa gctcattgcc atagcaacaa
      541 cgcatgccag taccattcct gtcatttcca cattgccgat cttcagtgca atgccggaaa
      601 gccctgttac aaatactacc gatgtgagca ccagatttct ggaacggctg taatctactt
      661 ttccgtctac cagcagacgg ataccggatg taccgatcat accgtaaagc aggaaggata
      721 cccctccgat aaccggaccc gggatcgtgc tgatcagtgc ggagagctta ccaaggaagg
      781 aacagcagat ggataaaacg gccgccccgc cgatcacctg cacgctgtag acaccggtca
      841 tcgccatgac accgatgttt tcaccgtatg tcgtggtcgg caccgatcct accagaccgg
      901 aaagcatggt ggaaaagaag tcaccgaaca gagtcctgtg cagtcccggt tcttttaaaa
      961 gatctcttcc gatgatctca cttgtcacaa cctgatgtcc gatatgctcc gacacgacca
     1021 gaagcagtgc cggaaatacc gtcatgatcg cttccaggtt aaacttcgga tactggaaat
     1081 tcggcagtgc gaagaaggat gcctgtacaa cttccgtgaa atcaaggata ccacacgccg
     1141 cagctgccac atagccggaa ataatcgcaa tcaggatcgg gatcacaccg aggaacccgc
     1201 gaaaaaggat ctgtccaaac acggcaacgc caagtgtcac cagaaaaacg atcacgtttt
     1261 tcggatcaat cacttcatcc agcaggccgg cattgcttgc ggcatttccc gcaagctcca
     1321 gcccgatgag ggctacaacc ggtcccatgg ctgctgtcgg cagcacgata tgaatccatt
     1381 ttgtgccgaa ttttcgaata atcagtgcaa caatgatacc gccaaatccg atggcaacaa
     1441 agcttcccag tgcatactgg taaccgaatt tctcaatgac cagccccgcc ggaaccagaa
     1501 atgcaaaact ggaacccagg tacgccggtg cttttccttt tgttacaacg ataaagacca
     1561 gtgtgccgac accgttcatc agaagcacga tcgccggact gattccaaac agaaacggta
     1621 ccagcaccga tgctccgaac atagcaaaca tatgctgaag actcagcggg atcagcatct
     1681 ttacaggtac tttctcgtcc acctgaatga tttttttctc cattttttct ttctcctgtt
     1741 atttttataa cacaggctct tatttgatcc ttgcatgcag atcctgcagg gaaacaactt
     1801 ctctgctgct ctgtttctgg accgtcagga taccttctgc acttgcaaga gctgctgcca
     1861 tggtcgtaat atacggaaca cggctcttga tgcaggctct tctcacatag ctgtcatccg
     1921 gtccctcttc ttcacccttc ggtgtattta caacaagatc gactttgccg ttagtgatga
     1981 tatcatagat attcgggcga ccctctgaca tcttgtatac aaactctgcc ggaatgcctg
     2041 cttcttcaat gatcgctttt gtatgcctgg ttgcaatgat cgcaaaacct gcttcgtgga
     2101 aacgttttgc cacttctgca acttctgctt tctcacggtc gccgacactg atcagaacgg
     2161 ttcctgagag cggaagcgga ctcttggttg cttcctgtgc tttgaagtaa gcttcgccga
     2221 cgctctgtgc cagtccgagc acttcacctg tcgaacgcat ttccggtcct aacagcggat
     2281 ctacttcctg gaacatattg aatggaaaga ctgcttcttt gacaccgtaa tacggaatct
     2341 cgcggtcttt ctgatctttg atcggtgact ctttcccggt aaactcagaa gtgatgatct
     2401 cggttgccag cggtaccata gagattgcac atactttgga aaccagcggc accgttctgg
     2461 atgcacgcgg atttgcctca agcacataga caacgccgtc ctcgatcgca tactgcatat
     2521 tcatcagacc tttgacatgc atctcttctg caattttctt cgtgtactct ttgatcgtct
     2581 ttaagttttc ttcggaaatg tgtctggacg gaatgatgca tgcggagtct ccggaatgta
     2641 caccggcaag ctcgatatgc tccataacag ccggtacgta agcatgttcg ccgtcgctga
     2701 tcgcatctgc ctcgcactcc attgcatgac gcaggaaacg gtcgatcagg atcggacggt
     2761 ccggtgtcac accttctgct gcttccatgt aaattttcag attttccgga tcatatacaa
     2821 cttccatgcc gcgtccgcca agtacatagg aaggacgtac cataaccgga tagccgatgc
     2881 gttctgcgat cgccagtgcc tcatctacat ctactgccat gcctgcttcc ggcatcggga
     2941 tctgaagttt gtccatcatt gcacggaaca ggtcacggtc ttctgccata tcaatgacag
     3001 ccggtgaagt accaaggatc ttcacgccgt atttttccaa ctctgcggaa aggttcagcg
     3061 gtgtctgtcc gccaaactgt gcgatcacac cgagcggctt ctctttcttg taaatgctca
     3121 gaacatcttc caatgtcagc ggttcaaaat acagtttatc agatgtatcg tagtctgttg
     3181 ataccgtttc cgggttgcag tttacgatga tcgtctcaaa tccggccttt ttcagagcca
     3241 ttgcagcatg tacgcagcag tagtcaaatt cgataccctg tccgattcgg ttcggaccgc
     3301 cgccgaggat catgatcttg ttcttattgt cacttgcttt gctctcatcc ttgatatgat
     3361 aagaagaata gtaataagca ctgcctttgg ttccgcttac gtgaacacct tcccatgcct
     3421 cttcgatgcc gtagccgata cgtgcattgc ggatttcttc ttcggtcact tccaggatct
     3481 ggcttaaata tttatcagag aatccatcca gttttgcctg acgcagagct tcttctgaag
     3541 gaacactgcc tttgtctgca agcagagctt cttcttcctc caccagctct ttcatctgct
     3601 cgataaaata atgtttgatc ttggtcagct caaacagctc atccacggtt gcatgtttac
     3661 gcagtgcctc atacatgatg aactgacgtt cgctggtcgg aacagacagc attttcagca
     3721 gttcttcctt tgacttttca tggaaatctt tggcaaatcc aaggccgtaa cgtccgatct
     3781 ccaggctgcg gattgctttc tggaatgctt ctttgtaagt cttgccgata ctcatgactt
     3841 cgcccactgc acgcatctgt gtgccgagtt tgtcctgaac gcctttgaat ttctcaaatg
     3901 cccatcttgc aaatttaatt accacatagt cgccgtccgg cacgtatttg tccagtgtgc
     3961 cgtatttgcc gcacgggatc tcatccagtg tcagaccgga agccagcatt gcggaaacca
     4021 aggcgatcgg gaatccggtt gctttacttg caagtgcgga agaacgggaa gttctcggat
     4081 taatctcgat gacgatgata cgtccgctct tcggatcgtg tgcaaactgt acgttcgtac
     4141 cgccgatcac ttctacttct tctacgattt tataagcctg tctctgcagc tctttctgaa
     4201 catcttctga gatcgtcagc attggtgcag aacagaaaga atctccggta tgcacgccaa
     4261 gcgggtcaat attttcaatg aagcatacgg taatcatgtt attctttgca tcacggacga
     4321 cttccagctc cagctcttcc catccgagaa tggactcttc gaccagaacc tgtcccacaa
     4381 gactcgcctg cagaccgcgt tcgcagaccg tctttaactc ttctacgttg tataccagac
     4441 cgccgccggc tccgcccatg gtataagccg gacgcagtac caccggataa cccagtttgt
     4501 ctgcgatcgc cagagcatct tccacgctgt aggcaacttc actgcgtgcc atctcaatac
     4561 cgagcttgtt catggtcttt ttaaactcaa tgcggtcttc tccacgttca atggcatcta
     4621 cctgtacacc gattactttc acatcgtaac ggtccagaac accggctgct gccagctcag
     4681 aacagaggtt tagtccggac tgtccgccaa ggttcggcaa aagggcatcc ggacgctctt
     4741 ttgcaatgat ctgctccagt cgttccacat tcagcggctc gatgtaagtg acatccgcaa
     4801 tctccggatc cgtcatgatc gttgccggat tggagtttac cagaatgatc tggtatccaa
     4861 gctgacgcag tgctttacaa gcctgtgtac cggagtaatc aaattcgcat gcctgtccga
     4921 tgatgatcgg gccggaacca ataatcagta ccttgtgaat atcatttctt ttactcatat
     4981 gcttctcctg tcgttttgtt ttcttacgta catcctgttt ccgtctccga ctgctgtacg
     5041 gccggagacc tacgtggtcc ttgcagacac aaatccgcat gcatacatcc gttttgtgcc
     5101 cacttgtccc atgcctcgtt ttattataca tgacaaccgc acaaaaatct actgcaaaaa
     5161 acagacagag aaatttcttt ttctctctgc ctgtttttgc gtcaatatcc tctttaatcc
     5221 tggaactttc caagaaattc tttgattcgc tgattgtccg atgtaaacag ttcttccggt
     5281 gctccttctt tcacgatcac gccattttcc ataaatacga tatgatctgc cacatttttt
     5341 gcgaaattca tctcgtgggt tacgatgacc atcgtcatat gttctgccgc cagatctttg
     5401 atgaccttta aaatctcccc ggtcagctgt ggatcgagag ctgaagtcgg ctcatcaaag
     5461 aacaggatct tcggattcat gcacagtgcc cttgcgatcg aaactctctg ctgctgtccg
     5521 ccggataact gatacggata ggcatctgcc ttatcttcca gtcccatttt tttcagcagc
     5581 actttcgcct gctcctgcac ttcttttttc tctcttttct gcacacggat cggtgcatca
     5641 cagatattct tcatcacgct gtaatgcggg aaaaggttaa agttctgaaa aaccagcccg
     5701 tagtaacttt tcagctgttt cagtttgccc ggtgcttcat agaccgcctc accgttctga
     5761 ctgtaagcgg cacgctcccc aagataagaa agctctccat catcgatctt ttcaagcatg
     5821 gtcgcacagc gaagcagcgt tgatttgccc gatccggacg gaccgatgat tgctacgacc
     5881 tgtccctctc tgacattcag ggaaatatct ttgagcactt ccagttcatc gaaatgcttt
     5941 tttatatgct tcatttccag ataattcata gattccctcc taacgataat agctcagatg
     6001 cttctcgatc ttctccatga tccatgcaac cagaaaatta aatacatagt agaaaatacc
     6061 ggctgccaca tacggaacca tactggtctg tgaagatgcc agtgcctttg cgatcgtaaa
     6121 catctccgat acggcaatca caaaggcaag agaagtatcc ttgaccagtg tgatgacttc
     6181 gtttgtcacc gacggaagga tgcgtttgat cacctgcgga agaatgatat gtaaaaatgt
     6241 ctgacttttg ctgtatccta aaatctctgc cgcctcatac tgtccaaccg gcatggattc
     6301 gattccgctt cgatagattt ccgcaaaata agctgcataa ttaagcacaa aaccgatcac
     6361 caccgcaatg tttcggtaaa acggtgaaat tttgatcccg aacagataat acggtccgaa
     6421 atagacaacc atcagctgca gcatcagcgg tgtgcctcgc atgattgaaa tataaatttt
     6481 tgtaatcccc tgaacaatac gtttttttga cattcgtcca aacgctatga ccataccaag
     6541 tggcagggaa aaaagcagtg tgatcccgaa aatccagcag gacgtcgcca taccctctgc
     6601 cagcttcata agcatctggc tgatacccat aataacttct cctttctttg cgtaaaacca
     6661 tgagaaatac actttggttc gttgtccgca taataagggc tgccgcagat gcaacagccc
     6721 gccttttgtt ctctgtctga tttactggat cgttgtgatg tcttttccga accacttctc
     6781 ggaaatcttt gcagccgttc cgtctgccgc catctcttcc agtgttgcct gaactttatc
     6841 acgcagcccg gtattacctt ttttgaagcc aatgccatat tcttctgctg cgatcacatc
     6901 atccaggact ttgaaatctg ccttacggct tgtgatctga taacttgcca caacgtcatc
     6961 cattgcgatc gcatccactg ccccggactc cagatccata aatgccgtgt tgtaatctgc
     7021 aaccggtgtc aggcttccaa acatggaagt cagctcactg ttgtcgttca gtgctgcttc
     7081 cgcactggaa tccgtctgga cttctacgtt ctttccggcc agatctgcct cagaattgat
     7141 gtccgaatct ccacgtacaa cgaagacctg attgtttttc atataaggct tgctccactc
     7201 atagtcgtct tcacggccat tcatggtgaa gccattccag atacagtcaa tcgttccggc
     7261 atccagctcc atgtcttttg catcccaggc aatcggctga tatgcaatcg taaggttcag
     7321 acgttttgca acttctgctg ccatctccaa nnnnnnnnnn taaactctcc gtcatctccg
     7381 acaaatccca tcggtgggaa atcctggtca aatccaacgg tgaacgtgcc atctgtcttg
     7441 ctggcatcgg tagtttctgc atctgctgcc tgtgtatcgg ctgatgtttc gtctccggaa
     7501 acagcacttt ctgctgttgt atctgctgcg gactgcgtct ctgcattctt ggaaccacat
     7561 cctgccaaca ttgccgatac cattgctgct gttaacataa cgctgactgc tctttttttc
     7621 ataatattgc catcctctct ctttttgtat ttcaaggggt cttcccctgt tttactgctg
     7681 tatgtgacca ctctaccgtg gtaaagtaat caaatactat cacattatca gggcgatgtc
     7741 aacttatctt ctgcttttat gatattttct tttttcttcc tgtaaaaacc ttgccagtgt
     7801 ttcctgaccg gcgttttcga tcttctgcat tttttctgcc tttgccagta cttcctgctt
     7861 ctcttcttcg atcttgtcga gctgttcttt tggcactttc agctcttcga gacgcttttt
     7921 atagtcttct tccgacagat acagcggcaa aattgccaga taactgcggt acaccgcatt
     7981 ggagcgtacc atctcataag actgccacag acagtctgcg gcctcgcgga acaggaacat
     8041 ccttccgtac gcccccgcca tattgctcag tacgcctgca taaaactgaa gccctgtttt
     8101 cccctgattg cggttttgca ggatctgacg gtaaaggctg accgccccgg aatacatgcc
     8161 gtaattgacc agcagatccg cacggatctt cctgcggata tcctccgtct gtacctccag
     8221 cttgccaatt tgctctttta ccgcatacat ctcagccgca ttcagataac gtacttcccg
     8281 gaaaaccggc agcaccagct ccgcaatact ctctgcgtct ttcagtttct cacgaagatg
     8341 gaacgccagc ctttgcatgc ccagctctcc ttccagccag tcacacagtt tttcattcag
     8401 aatgctctca tccaaaagac ttacattctt ccagagataa aagcacaatt cttctattgt
     8461 ataaatattg atatcaatac tctcgatata aaacggatgt ttcgccgctt tcgcacgaca
     8521 caactgatac attgccatca ttcctccctt tcctcccctt ttcaggaaag gctgatattt
     8581 gcttcccaga ttttaccgga agacggatac agttcaccaa gtccaagatc ctccgcccgc
     8641 accctgcatc tgtccggtgc ggtaaaggaa atgtcgagcc gcagtctggt tgcccgtctc
     8701 ggtcgatccg gaagctggct aagagccagg gtgtgttcca tttcttctcc
//
LOCUS       DAWDWH010000076         8619 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_4002_length_8619_cov_13.507356, whole genome shotgun sequence.
ACCESSION   DAWDWH010000076 DAWDWH010000000
VERSION     DAWDWH010000076.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8619)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8619)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8619
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4002_length_8619_cov_13.507356"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2610 a         1631 c         2274 g         2104 t
ORIGIN      
        1 agggaggcag ataccctggt tgtaaaagga cagaagcagg tgggaaggcg gtcctcaaac
       61 acaaagggca gtgagaatat catttcaaac ggttccggca ttgcagttgc agacggtcag
      121 tgtatgatga tcgttgaaca gggcaaaatt gtggaagttt gtgcagaacc gggagagttt
      181 acttacgata gctccacaga accaagtatt tttaccggaa aactgggcga aggtctgaaa
      241 aagacctttg ccaccttcgg aaagcgtttt acctacggcg gagataccgg aaaagaccag
      301 agagtgtatt atatcaatac aaaagagatc atggataaca aatttggcac ggcaaatccg
      361 tttttgttcc atgtagtgga tcacaatacc ggactgagcc gtgatgtaca ggtaagatgt
      421 aacggtatct attcttaccg catcaccgac ccgatccttt tctataagaa tgtctgcgga
      481 aatgtagaga tgacttacga cagagaagag atcgacggac agttaaagac agagtttatc
      541 tccgcactgc agccggcatt tgcacagctg tcaagactgg agcttctgcc aagtgatatc
      601 ccggcacatg cgggagatct cgaagaggcg atgaacgaag cactcaccaa aaagtgggcg
      661 gaaaaacgcg gtatttccgt ggtatccgta gcgatgaacc cgatcaccct caaagaagaa
      721 gacctcaaga agatccagga aatccaggat gctgctgttc tgagagatcc gacgatgatg
      781 gcggcaacgc ttggcagtgc acaggcagaa gccatgaaag cagccgcatc caatccaaac
      841 ggtgcgatga tgggctttat gggactgaat atggcaggag gagctggaag tgccaatatc
      901 caggatcttc tccagatggg aaatcagaga cagcaggcac aaagacagca gcagtatata
      961 ccggaagaaa aacaggccca gccgatacgt ccgtccgcac aggcaggaag ctggcagtgc
     1021 agctgcggag ctgtaaatac cggcaaattc tgcatggaat gcggaacagc aaagccggaa
     1081 gagggctgga gatgcagctg cggggcactg aacaaaggta aattctgcag tgaatgcggc
     1141 agtccgagac cagcagcagc aaaaaaatac cgctgtgata aatgtggatg ggagccggaa
     1201 gatccgacaa atccgccgcg tttctgtccg gaatgcggag atccgttcaa cgatggtgat
     1261 attgtacagt aaggggatga atctatggca gatatcagag aatacaaatg ccctgcctgt
     1321 ggcggagcaa tggaatttga cagcaaaact cagaaaatga aatgccctta ctgtgatacg
     1381 gttgcagatc tgtccgactt tgaagaaacg cagtcggaga aaacagcttc caaagaaggg
     1441 gcagaacagg gagaaacaca ggaagcagca gaagaaggag caatgatgct ctacgtgtgc
     1501 gaatcctgcg gtggtgaaat cctggcagat aaaacgaccg gtgccacgag ctgtccgtac
     1561 tgcggcaaca aagttgttat gaaagggcag tttgacggtg ccttaagacc ggattatatc
     1621 attccattta agaaagacaa aaaacaggca aaagaagcat accggaaaca tctcgaaggc
     1681 aaaaagtttt tgccgggtgt tttcaaacag cagaaccata tcgacgagat caaaggtgtt
     1741 tatgttccgt tttggatatt tgatctgaaa gcaaaggcag atttctccta tcatgcggaa
     1801 aagacgcgtg tatgggtatc aaatgatatt gagtacacag agcatgagaa ttttgaagta
     1861 aaaagagcag gaacggtaga gtttgaaaaa gttccggcag acggatcaaa aaagatggat
     1921 gatacgctga tggaatcggt ggaaccatat gattttaagg aagcagtgcc gtttcgcatg
     1981 gcgtatatgg caggatatct ggcagaccgc tacgatgtgc cgaaagagga gtgcatcggg
     2041 cgtgcgaaaa aacgtatccg gagaagcaca gagcgtgaat tccgaaatac cgttaaaggt
     2101 tatcatgtga tcgatcagga aaaagcaaat atggagatca aagaccaggc aaagtatgct
     2161 ctctatccgg tatggatctt aaatacgacc tggaaagata aaaaatacat ttttgccatg
     2221 aacggtcaga ccggaaagat tgtcggtgat cttccggcag acaatggagc attctggaga
     2281 tttgttgcca tgtggggagc agggatcgga cttgtccttt atgcactgct ctgggttttc
     2341 atgttgctgt aaggaggtgg cgagatgaag aaaacaaaaa tgaaaatcat gtctctgctg
     2401 cttagtatgg tattattttt attcgtacca tttctgaatc ttcaggcagc atcgaatcat
     2461 ccggcaagac ttgttgacca ggcagagctg ctcacggcag aagaacagca gcagatccag
     2521 gcaaaactgg atgaagtcag tgaattgcat cattgtgatg cagtggtaat gaccgttaat
     2581 tccctggagg gaaaaacggc aaccgaatac gccgatgatg catttgatta cgatggctat
     2641 ggacttggcg aagacaaagc ggggatactt ttcctggtgg atatgggcga ccgcaactgg
     2701 gcaacaagca cacatggtta tgcgatcacg gcatttacgg atgccggact tagttatata
     2761 gaagaaaagc tggtatccta ccttggtgat ggtgagtacg cagaagcatt taattgtttt
     2821 gcagaacagt gcgaccgttt cctgacacag gcagagacag gcgagccata tgatgtagat
     2881 aatatgccgc aagagccatt tgcctatgca ttctggctgg ttgtatgcct ggcaatcgga
     2941 tttgtgattg cactgatcac ggcatcgatc aaacgttcca aactgacttc cgttgtgaag
     3001 cggaccacag cagaagaata tatgaaaaaa ggcagtctga atcttaccgt gaaacaggat
     3061 cgctttatta ataaagtggt tacaagccgt ccggttccaa aaaatgataa tgactcacac
     3121 agtggaggcg gcggaagcag cacgcatgtc agctcatccg gtgaagtaca cggaggtcac
     3181 agcggaaaat tctaaagagt gagtatacct gcaaaagtgt gatacatttt tgcaggtatc
     3241 tttatataat tttaatgtgg ataaaaaatt gttaatacta tttttaggta catggtgtta
     3301 tgatagtcat gaaacagaac tgggagacaa aaagaatcat gaagataaag aaaaaagccg
     3361 gacaccggca tatgacatcg tttcagatca ttatactggg atttatggga gtgatcctgg
     3421 cagggagttt gctgctgatg cttccgtggg catccagaag cggaagatgg agttcctttt
     3481 ctgatacgtt atttactgcg acatcagcag tctgtgtaac aggactggtt gtgcaagata
     3541 cagcaactta ctggtctgaa tttggtcagg ttattatttt gctgctgatc cagattggcg
     3601 gtctcggcgt tgtgactgtg gcagtatcga tcgcaacgat cgccggcagg aagatcggac
     3661 tgatgcagag aagtaccatg caggaagcaa tcgcagctcc gaaaatgggc gggattgtga
     3721 ggatgactac ttttatctta aaaacctcgt ttttgatcga actgatcggg gcagttattc
     3781 tgtttccggt tttttacaga gaatttggac ttggcaaagg aatctggtac gctgtatttc
     3841 atgcgatatc tgcattctgc aatgccggat ttgatctgat gggagtaaca ggaaaatttt
     3901 catccctgac ttcttttgcg gcaaacccgg ttgtgaatct tgtgatcatg acactgatcg
     3961 tggttggcgg tattggattt tttacctggc tggatatcaa ggagaataaa tggcatatta
     4021 aaaaataccg gctgcagagc aaagtagttc ttgcagtcag tgcctttctg atcctggttc
     4081 cggcgatcat gttctttttt ttggaatttc agcatatgcc gctgggagaa cggatctgga
     4141 gttctttttt ccagtctgtg acaacaagaa cagctggatt taatactgct gatctcgata
     4201 aaatgacaga ttccggaaag atgggaatgg tgcttctgat ggtgattgga ggatctccgg
     4261 gatctacagc aggcgggatg aaaacaacaa ccgcagcggt tctgtttgcc atggcgattt
     4321 ccgtatttcg aaagaatgag caggcacatc tttataacag aagaattgca gaagagacgc
     4381 agcgaaatgc cgctacaatt tttgttttat atatgatgct gtttacagga agtgcgatgt
     4441 tcatcagcta tctggaaaaa gtaccgcttg tgacggcatt ttttgagacc ggatcggcaa
     4501 tcggaacggt aggactttct cttggactga cgccgacact ttgtctggct tcgagactgg
     4561 tactgatctg tctgatgttc tttggaagag tcgggggact gacgctgatc tttgcgaccg
     4621 tttccgggaa gcggacggag tataaatatc cgcaggaaaa gattaacgta ggataggagt
     4681 atccaccaga tacagagtgt gcttgatttg caggaaaaag caggatattt atgatgcgat
     4741 caagatgaaa aatgaaaaaa gctatagatg gaggaaaaga agacatgaaa tcaatcttac
     4801 tgatcgggct tggaagattt ggaaggcata ttgcgataaa actggatgaa ctgaatcatc
     4861 aggttatggc agtagacaac aatgagagca gagtagaggc agttctgccg tatgtgcgta
     4921 acgcacagat cggggatgcg accaatgagg attttatccg ttcactcggc gtgcgcaatt
     4981 tcgatgtctg cattgtggcg atcggagata atttccagag ttctctggaa acaacgtctc
     5041 tgttaaaaga gctgggagca aagatggtag tctccagagc agcaagagat gtacatgcga
     5101 agttcctttt aagaaacgga gcagatgagg ttgtatatcc ggagaaacag ctggcatcct
     5161 ggacggcgat ccgttacagt gcagatcaca tttttgatta tgtggagcta gatgaagaac
     5221 atggcatttt tgaaatttcc attccggatg cctggatcgg aaaaacggtt ggagaactgg
     5281 acatccgaaa gaaatataat gtaaatatca tggcactgcg gtgcaacggc gtgctggata
     5341 tgaatattgc gtcggaaact ctgcttggag atgaccagac catgctggta ctgggaaata
     5401 ttaaaaacat ccagaaacag ttccatatct gaaacgaaag aagatggtaa tatgaaagaa
     5461 tttcttttat tgccggcagt gggaggcatt tttatattta tgtatgaaat gctgaaaaaa
     5521 tttgatgcat tttgtgatgc aaacagacag aatctctggc tggaggagga tgcggtaccg
     5581 gaagaaaaag aggaaagtgc agaacaggtg atggatgact gcctttcctc ttctgtttcc
     5641 gcagaacgga acggccgcct ggaaaaactg ctcgctgcat ttcagagaga aaagaaagta
     5701 cagaacttcc gtgatctgtt tactagtatt ttgatcttac tggcagccag tgtgatggga
     5761 tattttttca acagtctggg ttttgcaaat gccaatatca tgacagtgtt tgtgtttgca
     5821 gtacagctga tcgcagtgct tacaaatcac aggacctaca gtatgatcgc cgccgtgctg
     5881 agtgtactta tttttaattt tctgtttacg acaccgcggt atacatttca tgcttacgga
     5941 gagggatatc cggttacctt tctgatcatg ttcggtattg catttttgac cggaacgctg
     6001 gcactgaagt taaaaaatca ggcaaaacag tcagaaatgg tggctttccg cacaaagatt
     6061 ttgtttgata caaaccagat tctgcagtgt gccagaggaa gggaagaaat catcagtaaa
     6121 acgggacagc agcttcggaa actccttgga agaaatgtga ttttttacag tgtaaaagat
     6181 catgaactgg aaaaaagcaa agtgtttatg atggaagaca gggagtggtc agaacagcag
     6241 aagctgaaaa aagaaaagta tgtggcagaa tgggttttaa aacaccggaa acgtgcaggt
     6301 gcaggcacag ggaatttccc gggtgcagaa tgcctttatc tgacggtcgg tgtaaatgaa
     6361 acagtttatg gagtactggg gatcagcata gaaaagaagc agctggaaag ttttgaaaaa
     6421 agtatccttc aggcgattat cggcgagtgt gcactggcac tggagaatga acagattacc
     6481 attgaaaaag aaaaagcggc gattctggca aaaaatgaac agcttcgctc aaaccttctg
     6541 cgggcgattt cacatgatct gcggacaccg ctgacctcaa tttccggaaa tgcgagcaat
     6601 ctgctttcca atgcagatta ttttgacaaa gagacgaaaa agcagctgta tctggatatt
     6661 tatgatgact ctatgtggct gatcaatctg attgaaaata tgctgtccgt gaccagactg
     6721 gaggaaggtc ggatgaatct gaacatatca gtagaactgg tggatgatgt gatccaggag
     6781 gcacttcgcc atgtagaccg gaaaaaagat gaacacacga tcacggttga gcatgaggac
     6841 gaattgcttc tggccagaat ggattccagg ctgatcgtgc agatggtcat caatctggtg
     6901 gataatgcga tcaaatatac gcagaaggga tcccatattg atatcaggac aggaagagaa
     6961 gggaaaaatg ccgtaatctc agttgcagat gacggacctg gaatttccga tgaaatgaaa
     7021 gaacatattt ttgaaacttt ttataccggg acgaataaga ttgcagacag caggagaagt
     7081 cttggacttg gacttgcact ctgcaaatcc atcgtaaatg tccatggagg agaaatcaaa
     7141 gtttcagatg cacatccaca tggagcagtt tttcagttta cattaccagc aggggaggta
     7201 tatttgtatg aacaaaccac ggatactggt tgtggaagat gataattcgg tgaaaaatct
     7261 gatcacaaca actttgaaag cacatgatta tcattttctg acggcggaga atggagaagt
     7321 ggcgatcctg gaagcagcga gccacaaccc ggagatcatg ctgctggatc ttggacttcc
     7381 ggacatagac ggaattgaag tgataaaaag agtgcggaca tggtcgcaga tgccgatcat
     7441 cgtgatcagt gccagaagcg aagatgttga caagatcgaa gcactcgatg cgggagcaga
     7501 tgattatctg acgaaaccat tttcagtaga agagctgctt gcaaggcttc gtgtgaccag
     7561 acgcagactg cagatgatga aaagtgacgc actgaatcag agtgcagttt ttgtaaacgg
     7621 agatcttcgc attgattatg cggcgggctg tgtgtatctt ggggaagaag agcttcatct
     7681 gacaccgatc gagtataaat tgctgtgtct gctggcacag aacgcaggaa gagtcctgac
     7741 ccataccttt atcatggaaa atatctgggg cagtaactgg gaaaaggaca ttgcttcgct
     7801 tcgggtattt atggcgacac tgaggcggaa actggaaaaa aacccacagt ctcttcagta
     7861 tatccagacg catgtcggcg tcggataccg tatgatgaaa tttgaagaat catcagaaaa
     7921 taaaaagggt gaatattaaa acatcaggat ggagtctctt ttatcctctt ccagcaagaa
     7981 gactccgacc tctaaggtgg gagatggcaa gtacaatgga aaagaaaaca gtatgcaggg
     8041 agatcatccc ggcatactgt ttttttaacc aaatcaagca gcaaagtgga ttgcgaatat
     8101 tcgctttttc tattctacag aatttcctgt aaacgcttca gcagatcgct ctgcagatag
     8161 taggtagtct caaaattcag ataacgcatc gtctgatcat cccacaggat tttaatctgc
     8221 ggcgggaatt cctcatcgct ttcccagaac tgaagcagaa ccgggaagaa agaaaatgcc
     8281 gggatttctg cagtgatatc agcaccggca agagctggct ttaaaatccc gccgatggaa
     8341 acacatgcct ttttgacggc atccactttg ccggaaaaga aatctgcata tttctgtgaa
     8401 aaaatttcgg cacttgggct tgcaccggca gcagcaaaat ttgcgattgg agtccatttg
     8461 ccgccaagag ggggcagttc cgtttcttga ccgtgacaga gcatatcgta gatggtcatg
     8521 actgtttcat aagagcgaca ttctgcaaaa ccatccgatg ttttttcgaa aacagttccg
     8581 cttgggcgat caatcttgca gggcgtattc agataagta
//
LOCUS       DAWDWH010000077         8608 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_4007_length_8608_cov_14.991231, whole genome shotgun sequence.
ACCESSION   DAWDWH010000077 DAWDWH010000000
VERSION     DAWDWH010000077.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8608)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4007_length_8608_cov_14.991231"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2547 a         1740 c         2293 g         2028 t
ORIGIN      
        1 atggcagacc agatagaatg tcagatggag gagctggcac aggtttctga gcgaaggaaa
       61 cagcttcttg gcagtctggc acacgagctg aaaacaccga tgacttccat catcggctat
      121 tcagatacgc ttctgcatgt gaggatcaac gaagaacagc agaaaaaggc actgacgcac
      181 atcaacaacg agtgtcgccg tctggagcga ctttccggga agatgatgaa tctgctggag
      241 ctttacaaca acgagacgat ccatatggag aagcatccgg tcagccagct gatcggaaaa
      301 gtggcggagc ttgagaagta tcatctgaaa gaacagggga tcacactgga aaccaggagc
      361 gatgacaccg ttctgctcat ggacatggat ctgatggaga gtcttctggt gaatctgatc
      421 gataatgcca tgaaagcttc caaagaaggt gataccattt atgttgaagc agccggaaca
      481 tgcattctgg tggaagacca cggaagagga atcccggcag aagagattcc gaagatcacg
      541 gaggcttttt atatggttga caaatccaga agcaaaaaag caggcgggat cgggctgggg
      601 cttgcacttt gcacacagat cgtgaagctt catggtgcaa agatggagat caaaagcaaa
      661 ccgggcgtcg gtacgcagat ccgggtgatc ttttcggaag aacagtaagg agaaaaaatg
      721 aaaagggcaa aaaaaagaat cttacgaagg atcgtatgga tcggaatgat cctgatggct
      781 gcaggatttc tgaccggttg tatggaaaca tcggaagatg actgtattgt aaaggaaaaa
      841 gagacagaga ccggagacag tatccgttct tattcttccg gcacagaggc agtggagaat
      901 gtcagcgtag cagaacaggt acaggcagcg agtccttgta tgctgagtgt ggaagaaggt
      961 aagattcatg tggatcttca ggcgagtgtt tatctcccgc agggaaacag catgaagatc
     1021 tacaaggcaa agacacgcta tttcacacag gaagatatga atggctggat ccatatcctg
     1081 acgcagggac agccgctgaa aaacgacaag ggagatacga tttcctggcg ggcacagcag
     1141 gaacttaccg gaacggtgaa gatgacagat ccgtatgctt cctgggcggc ggcaggcaag
     1201 aaagtgtatc agtttgaaat gcagaatctg ctggaagatg gctacaaaga atccggtgtt
     1261 tcactcagaa gggaagaatg tgaatatgta ccgtggagca gggatgacag ctggcaaatt
     1321 ctgcatacaa aggttggaag aaaacggatg agaaaagaag cagatgctct gcttcagact
     1381 ctgggacttg cagaagagtt tcattttatg cagtcacggg cggtttactg tacggaagat
     1441 tctgtgaatg aaaataccgg ggaagaaacg gaaacaggcg ataccggagc ctcggagcag
     1501 aaaatgggct ggaggtttta ctatacaagg caggtggacg aagcacggat caaccagagc
     1561 acagaccaca gcctgtcagg ggatatcctg ttcggactgg gcggcaatct ggatatggct
     1621 tccccggaaa acgagggaga gggatacgac ggaacaggat cagggacgga aaactggaaa
     1681 ccgagccggg aagccgaaag cttttatatt acctttgatg atgaaggtct tgcagaagtg
     1741 acctggatca acccatgtat cgtagaagaa aatgagggcg gaggagtttt tctgcttccg
     1801 ttttcagata ttctgcaggt ctttcagaaa tcgatcacca aacagtatat tctcgagatg
     1861 ctctccggca acggtctgga ggtttccgca accattcaga cgatccggct tggataccgc
     1921 aggattgaaa ctgaaggcaa agacggaaaa aagaaaaaag agcagctcat cccggtctgg
     1981 gattttatcg gtacttacag cagtccgcag acggaacagc tgctggcaga tgcggatgat
     2041 ctctgggtgc agtgttacct gagacaggaa cggacagaca gtctgcttac gatcaatgca
     2101 aatgacggaa ccatcgtaga ctggggaaat gaattataat tacggttcag attgccttga
     2161 ggggaaaact ctgttatact cttactatct aggaagtgaa agaaggagaa atcgtatgca
     2221 aaaatatgga gaaatcaaag atcttgtaaa tgctgttctg gaaagttatg ataaatatcc
     2281 ggtcattcaa aacatcgact gccgcagccg gatcaacagt gagagtgtca gcgaccttct
     2341 ggaaatgatt cgcaagatcc tttttccggg ctattttgag ataaaaaatc ttcgaaaaga
     2401 ttcgatcgaa tatcatgtcg gagaacttct ggaaaacata gagtataacc tgaccaagca
     2461 ggtcatgatg gcactgccgc acagcagcaa gtaccgtgag gcggataaag agaccttgat
     2521 ggaaagtgca agagagatca cgcaccgttt tctggagaag atcccgaagc tgcgtgatgt
     2581 gctggcgacc gatgtccagg caggatacga gggcgatccg gctgcgttta acaccgatga
     2641 agtcatcttt tcttatccgg gtatgtatgc gatcacggtc aaccgtattg cacatgaact
     2701 ctatctgctc ggtgtgccgc tgatcccgcg tatgatgacc gaacatgcac acagcctgac
     2761 cgggatcgac atccatccgg gagcatcgat cggagaatat ttttttatcg accacggtac
     2821 cggtgttgtg gtcggtgaga ccacggtgat cggaaaatat gtcaagattt atcagggtgt
     2881 gacacttggt gcactttcca ccagaggagg acagagcctt cgcggtgtga aacgtcatcc
     2941 gaccctggaa gacaacgtaa ccgtatattc cggagcatcg atccttggcg gagagaccgt
     3001 gatcggcacc ggagcggtga tcggaagtaa tgcatttatc acatcttccg taccggctca
     3061 gacgagagtc agcatcaaaa atccggaact gcagtttaaa gaccgctcca gaaaggcatc
     3121 tgagcttggt caggaaggat tcttcaaccg caaagaagag gactaaagaa aaggagtagt
     3181 atcgtgaagt tttttcatat agcagataca cacctgggag cgatcccgga tgccggtttt
     3241 gcgtggagtg aagaacgcag aagcgagatc tgggagagct ttctcagtgt tgtggcaaag
     3301 gcagatcgag aggaagtgga tctgctgctg atcgcaggag atctgtttca cagacagcca
     3361 ctgatgcgcg agctcaaaga agtcaactat ctgttttcca cactgaaaaa aacaaaagtt
     3421 gtgtttatca taggaaacca tgattacctg aaagtagact cttactatct ggattttccg
     3481 tggagtgaga atgtgacctg cctccggggc aaggaatgtg aaagtgtcgt gtttgaggat
     3541 ctggatacgg aagtgtatgg attaagttac catacaaggg agatcaaaga gccgaaatat
     3601 aatgatctgc agccggagaa aaaggccggt ttttccattc tgctcgggca cggcggagat
     3661 gcaaagcata ttccgatcga ccgcaaaaag ctgcttctgt cgggctttga ctatattgca
     3721 cttggacata tccacagacc agagatgata gagccggacc gtatggcgta tgccggggcg
     3781 ttagagccga tcgaccagaa tgatatcggt cctcatggct ttattgaagg ggaatataac
     3841 gaaggcaagc tgagtatttc gtttgtgccg tgggcgtgca gagagtatat cccacttgag
     3901 ctggatacga aagaatgtcc gaccaacctg gcatttcagg agacagtgcg gatgcggatg
     3961 gcagcaaagg gaatgcagca tatttataaa gtgcttctgc agggatatcg tgatccggat
     4021 attgtatacg atctggatgc ctgcaggaag cttggaaata tcgtaagcgt acaggatgag
     4081 agcgttccgg actatgattt tgaacggctt atgcgtgtcc atgcgggcga tattgccgga
     4141 agatacatcc agactctgta tcataaagac atgagtgata cagagagaaa ggcattgtat
     4201 tacggaatac atgcactgct ggagatgaga ggataaaagt acatgcggat actggaatta
     4261 gaaatcatta attttggaaa attcaaccac gagacagtca catttcatga tggaatgaat
     4321 ctgatctatg gcgagaatga aatgggaaaa acaacgcttc attcatttat ccgcggtatg
     4381 tttttcggga ttgaaaagca gagagggcgg gcagccagaa aagatgaata cagcatcagg
     4441 gaaccgtggt ttcatgcagg acagtttgca ggtatcatgc gatttgagag cggcggcaaa
     4501 atctttcgtc tggagcggaa tttttaccgc aaggaaaaga gcgtcagcct ggtatgcgag
     4561 acaaacggtg aagtactgtc cgtagaaaag ggcgatcttc aggttctgat ggaaggcatg
     4621 aatgaggatg cttttcgcaa taccgtgttt tttaatcagc agtcggcagc gacggacgag
     4681 ggtctggcaa gagaactgcg aaattacatg accaatctgc agaattccgg agacggtgag
     4741 atgaacgtca gtgcggcgat caagagcctg gaaagccaga gacggcatct ggaatccgaa
     4801 cagaagcgtc tggatgcaga acaggcagaa gagctggaaa gccagcagat gcgtctggag
     4861 tacgtgcgtc aggaactgct ggagctgtcc gaagaacaga aacactgtga agtccagatg
     4921 gcacgcatcg caaaagagcg gatcgcactt caggcgagac tgacagaaaa aaagagaagc
     4981 ctggagaaaa tgaagcggga actggatatt ccgctggaag agaatgccgg taaagatgag
     5041 atggagtcct ggaaaatgcg aatggaagca ggacaggaca aacgcaggat gctgtggcgt
     5101 aaccaggagc tctggctttt aggaggcggt gtgctggcac tgctagtttg cattcttgtg
     5161 ccgcatatct ggatcagtgc ccttgtgctt gccggatggc ttgggatttc tgccattttt
     5221 gtatggaaga tctaccgccg ccatcaggag gagcaggagc ggcaggagcg tatccagaaa
     5281 cggatgcagg agcgtatgaa aaaagagatc caggccgagc gggagcgttc cgcaaagcgt
     5341 gagaagcttc tggaagaaaa ataccatgaa gaagatgaac tggctcagcg tgaaaaagag
     5401 ctgaacctgg aatttgaaaa gcagatgtgg aatcaggaac gcatcagtgc ggagagcaaa
     5461 gaaaaacagg ttattttaaa caatctggaa gagagcctcc aggaattgca ggaagagaat
     5521 gagcagagcg gaagtatcca ggaagaactc gatgcggtac gtctggcgat tatgacgatg
     5581 aaccagatct ctgaggatat ttacaaagaa tcggccgcac atctgaatca gaaaatctcc
     5641 gatattttaa gccagatcac agaaggacgt tataccagtg tatttctcga tgcaaacatg
     5701 gaagtacgta tcaatacgcc gaaaaagctg ctctccctgg aacaggtgag ccgcggcacg
     5761 atggagcaga tctactttgc actgcgtatg gcggtcggtg agatgttctg ccaggatgag
     5821 accatgccga tcatactgga cgatgcgttt gtgatgtatg atgataagag actgaaacag
     5881 acactgaaat ggctctatca cagcaaaaga caggtgatat tgtttacctg ccatggaaga
     5941 gaacagagga ttctggatga gataaagcag gagaactaaa tttttgaatg tgcaaatgaa
     6001 cgaattcccc gaaggcacgc acggatattt ttgtaagtgt atggaatgta taaaaatgag
     6061 agtatagaag tgtatagagt gccggacgaa aaaggcagaa cccgtgacac gcatcgtttc
     6121 ctgttcgatt tgtgccgtct atgatccgcc gttcgctgga actcgcagct acgctgctca
     6181 gacaccgctc ggcggcggaa actagacaca aatcttttta cttcactcgg aaaaagataa
     6241 agacataatt tttatacaaa ctgatctaaa acgatatcat agataatgac cggatgattt
     6301 gtgttgtaat aacatagatt atcaggttat tttatatcag atgtgcacag aagaatatgt
     6361 catgcgatgt atgatgtgca ccttgcagca agaaagccag aagcattctt gaacaaatat
     6421 acaatagaat tgtccgactt attgttgcaa ttttataaaa atattggaag ttgttagaaa
     6481 agtgtaaaat aagaagttgc tttcatttat ggagaaaaag tagtgcccac accttggtag
     6541 tatgaatcct gtttgctcat gtgcaaataa aatgcgtgat atttgaggta catgtggtat
     6601 gtaaataaga acagccataa tgattcgaaa acaaatcgta ctgacgatag caaggcttcc
     6661 gtcgtgaggt ttgcgatgtt tccgcccccg agccgctgtc tgagcctcga agaggcgagt
     6721 tcaggcaaag ggcggataac acacgagcga accgaacagg aaacgaagcg tgtcagaaga
     6781 ttgttttcga accattatgg cgttctgttt tccaaaacta tttcattctt aaatcacatc
     6841 tgtttgtgca ggacaaacat tatgaattgt gttcttttaa gaatttattg atccggtcta
     6901 atgggtttaa cagactgctg atggatgcat catgattcag cgtatcaatg atgtaagcga
     6961 tatacttgct gaggtcgcag ttgatataat acggtttctg gagcagctcc ggtgtctggt
     7021 ataccaggtt ggttgtcaga atgaaatcaa acatgccggc ttcataagcc ttgtcaaatt
     7081 tctccatgcc gtttgtgaac agaccgaacg tagagcagat aaatacacgg tttgctttgc
     7141 gttttttcag ttctgcagcg acttccagaa cacttccgcc ggaagagatc atatcgtcga
     7201 tcacgatcat atctttgcct ttcacatcag aacccaggaa ctcatgtgca acaatcggat
     7261 ttgtaccgtt tatgatggta gcataatcac gacgtttgta gaacataccc atgtcggttc
     7321 ccagaacgtt tgccatgtag atagcacgtc ccataccgcc ttcgtccggg ctgatgacca
     7381 tcatggtgtc tttgttcgga tgaagatccg gaactgcacg gaacagacct ttgataaact
     7441 ggtaaaccgg ctgaacggtc tcaaaacctt tcagagggat tgcattctgc acacgcgggt
     7501 cgtgagcatc aaaggtaatg atattgtcaa cgcccatgcg aaccagttcg ttcagtgcca
     7561 gtgcacagtc cagagattct cttccggtac gtttgtgctg acggctctcg taaaggaacg
     7621 gcatgatgac attgatacgt cttgcttttc cgcctactgc tgcgatgata cgcttcagat
     7681 cctggaaatg gtcatccggt gacatatggt ttgtatatcc gcagagattg taggtcatgc
     7741 tgtagttaca tacatctacc agaagataaa gatctgctcc gcggacggac tgacggatca
     7801 cgccctttgc ttcacctgag ccaaatcttg gcacttctgc gttgatcaaa taagaatccc
     7861 gttcgtaacc ctggaaagaa aggctgttct tgtgttcatg gtttcgctcc tgtctccatg
     7921 aaaccaggta gtcattgact tttttgccca gctctttatg gctgtccagt gcgatcagcc
     7981 ccagtggtcc taccgggatg gtttctatac atcgttcttt tgttgtctgc attttgttgt
     8041 tgtcctccta cgcatactta ctacatttta aatgaaaacc caatcttata catacctact
     8101 ttaacataaa gtaaagcaaa gtcaagccat tttcagccct gttcaccaag ctttttcatt
     8161 aagcggatat ctttgccgat cagtttccgc attttgaaat tactggaaat ccgcgaaaag
     8221 acacgctcag aatacagatc ccgcagagat tccagagaga gattggtgga aatgatcgtg
     8281 gatttcttgt tcatcagacg ctcgttaata caaaggaaca gctgagaagc gacgaatgta
     8341 ttcgtcattt ccgttccgag gtcgtcgatg atcagcagat cgcagtcaaa aatggcactg
     8401 tgctgctgca aaacttcatc ttcggcatat tttttgccga acgtggccgt tgagagcact
     8461 tcaaacaggt caaaagacgt aaaataaatg acagaatgtg cactttcaag cagttccttg
     8521 gcaatgcagt gcgtcagaaa ggttttgccg acgcctgtcg caccgtaaaa gagcagattg
     8581 tcatgttcca tatcaaaatt ctgtacaa
//
LOCUS       DAWDWH010000078         8546 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_4027_length_8546_cov_12.238370, whole genome shotgun sequence.
ACCESSION   DAWDWH010000078 DAWDWH010000000
VERSION     DAWDWH010000078.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8546)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8546
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4027_length_8546_cov_12.238370"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6517..6526
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8266..8275
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1795 a         2228 c         1772 g         2731 t
ORIGIN      
        1 tcgttccgtc atcctgagca ttttctgcca tctccggcgt gaccagataa ccctcgacat
       61 agcgtgccgg gattccgaag cagcggtaca tcagaacagc cgccgatgca taatgcacgg
      121 aatagccttt cttctccgtc atcagaaaat cggtcacaaa atttccggtc tttgcatctg
      181 ttttctgatc ttctccggat gcatctgtat tcttcggatc gatctcttct gtataagtga
      241 tgttctcatt taaatatccg gtaatcctgg tatttgcctc ttcataggaa atatgagacg
      301 taacattttc gctgtctttc tcatccgaat cagaagcaag cacttttgta agcatctgtt
      361 tcagagaatc cggtacctgc agatactggt catatacata tgcattgtag ctgttttcgc
      421 attttttgta ttctgcaaat gcctccgtat cctgatcctg ataatatgcg gatgcgatct
      481 gcggatactt tctgatcaga ttgctgtgta ccggaaacga aatcgtcttt tgcggccgga
      541 atccttccgg gatcaggcgg tcatctccaa aacttcggac attttccaga gtatccggaa
      601 gcgtactcag ctcatacggc acataagcat atttccggct cgcaccaatg gtcgtgacag
      661 tcatatttcc ggtgtcatcg tctgcaggat tttccagctg ataaagagcg gtaagctgct
      721 gaagcccgga aacgttttct ttgtgaagcc agtaaaacag atccttttta tcataaattt
      781 catctgcatc ctgttgtttc cagccgtctt ctgtgtaaat actgccgaca aatccacgaa
      841 gatagagaga atccggatgt tccatggtca ctttcagagc ggcttcatcg gtcagtttca
      901 ggtttccaag ccctcggaac tgtccttccg gcagggaatc caccgtcttt ttcccataac
      961 ggatctgttc tgcaaatttc tgtactttct gcgagaacgg attgtctggc gaaaaactcg
     1021 aagcttttcc ccgaagcatg aaaaatccgg ctgccgtcac tgccgcaaat aacacaagca
     1081 gcatcacatc tgtctgtaac tgtgccagac ctttttcttt tttccgaaag gcaacaaggc
     1141 agagaaaacc tacatagatc agaagcaccg gaaaaagaac tgcttctttc tgccagatca
     1201 gaagcagaac tcccggaata aggctgacga gaatcgttcc gatgcagctt ttgctgaccc
     1261 ttaaaaattc cagaagagct gccagaacca tgcctgctgt tacataaaaa acggctgtaa
     1321 caaatggctg caaaggttca gaaatcgtaa gtgcataatc cggctcaaag gttcgcatat
     1381 ttctcccgat cgtctcaagc agcccgtttc cggcgatcag tgctccctga aagatccagc
     1441 cctttcggat cgtaaagctg atcagaaaac aaagaactgc cgctgccaga atttcatgca
     1501 cgattttctg tatgaatttc ttttcttttt gtccatttac aagaacggcc atcacaagta
     1561 gaatcgctcc cacactgcca gaaagagtca tcgccagaga tgccctgcaa aactgcatca
     1621 ccataagata ccatccgaca taaatgcaga aacaggcaag tgcttctccg acattttctt
     1681 tctggttttc cggcttctct gcaacctgaa cgtgcagttc tgtttcttgc tttttttgaa
     1741 tttttctgtg tttcttcatg tttcactcct aaatcagcag gataccgaca tcttctcctg
     1801 gccgtacggt ctggaaactg cactgccatg caaagagatc tgccagattt cccggatctt
     1861 ctgcggtaaa atagagaata tgtccaaatg gctgttcctg atatttctgt gcatattcct
     1921 gcaagcctgt gaatgttttt tcttttctct ccagtgaaag taagccgtgt atgatctggg
     1981 caaggttatc tgcggagtcg atttcttcca tacaaagctg ctcttttttc tgatcgtacc
     2041 agccgatctt gtgatgcatc cctttctccg tcagattttc ggaaacggag atcaattttt
     2101 ccatcagttc tgcctgatct gaagttctgc tatccccggt cagttttgct gtctcaaaaa
     2161 acagaagcac tgcattttct accggaagtc ccggttcctt cacataaagt tctcccagtt
     2221 tctgtgagat tttccagtga atcatcttcg tgctgtcacc tggctggtat tcccggatcc
     2281 cgaaggtttc tcccggatcg tccccttttt tctgttcgga atattgtatg ctttcccagt
     2341 cggtcgtaaa actgtctccg acctgtactt ccaccgtttt catttccgga agcacgatca
     2401 tggtatcgct gatgttaagc ttctgctcgg tcaaaaacag tcccagcatg tcactgacca
     2461 gaagtttctg cacggatatt ctgatggccc cgcagcagtt gctgcaaaca ctccatttcg
     2521 tttctgtctt ttctttcccc gctgccatac tgtcaagatg gcagatttct ttctctccgg
     2581 tcagcagatt ttgaaacaga agttctgccc ttaagcgtcc tgccggcagc aggctgtggt
     2641 tttcaaagat cagtttcccg taaacctgct gttctttttc tgccatgtcc ggcacttcaa
     2701 aatagcaggt tactttattt ttcatggcaa tcgaaagaaa aaagcagaaa aagccgagca
     2761 gaacggttgc cacagctgca attcctatgg tgatgctcgc acttaccaga tatgcaagca
     2821 ccagaaacag catccagatg ccgacaagga ttctggattt tttcatgttc tcctcctatc
     2881 tgatctccgg aatggaaacc tgatgtgtga tctgctccat gatctcctct gcctggatac
     2941 ccgcaacttt ggcacgcgga tgcaggatca cgcggtgtgc acataccttt gcaaatacgc
     3001 gaaggacatc atccgggatc acataatcac gcccgtttat gtaagcggat gcttttgcca
     3061 tgttgttgag ggccagtgct cctcgcgggc tgacgccgag ctggatcttg tcgttctgtc
     3121 tggtcgcttc tacaagcagt gtgatgtatc tgaggatatc atctgccact tctacctctt
     3181 ttacttcctg ctgaagctgc tgtacttctt ctcgggatga gatcatctga agcaaatcca
     3241 gcggctgctc cgtctgacga tcccgcagga tattgatctg gctttcaaag tccggataac
     3301 ccatggagag tttgaccata aaacgatcca tctgagactg cgggagcatc tgtgttccgg
     3361 cagaaccgaa cggattctgt gtggcaatca cagtgaatgg cttcgggatt tcataggttt
     3421 ttccatctac cgtggcattt ccttcttcca ttacttcaag cagggctgcc tgtgtcttgc
     3481 tggaagtacg gttgatctca tctgccagaa gcaggttgca catgactgcc cccggtttgt
     3541 actgaaactc tccactctgc ctgtcgtaca gggagaatcc gaccacatcg gaaggggtaa
     3601 catccggcgt aaactgaatt cggtgatagt ccacgcccat gacttttgca aagctcagtg
     3661 ccagcgttgt cttcccgact cccggaatat cttccagaag cacatggcct ttcgccagga
     3721 ttgtcatcag cacctgttcg atcacttctc tttttccaat gattactttt tcaatctcgt
     3781 ttatcattgc ttctgttttt ctcatatgtc ctctctttct cataactgtc ctgttatatt
     3841 tatactgttt actttctgca aaatccgtta aaattttctt cccaaccgta tttttgcaca
     3901 cctgaatcct gtttttgctg tcatggcatc ccatgcttct cctgtgccgg atcattcttt
     3961 tttgtttctt ctttttaaca tcagtaccac tgccgctgcg atcactgcaa tcactactgc
     4021 gatgaccatg accggcatgg cactgccatt tttcgaaagc agggatgctt tttccggacg
     4081 caagttaaat aaatttttca tattgttttt gtaacggcag aatgcggtca gtgcgtatgc
     4141 cgcctggcct cctgccagga aatcggattc atccggtatg gatgccggat tggacgcatc
     4201 ataaacataa gcatggacaa atccgccatc ttcctgacga aagctcatca gattatcaag
     4261 cacggtattt ccgtttttga taaaacgact gtcgctctgc ggatcgatgc ctagactgca
     4321 aagagccgtc agaacctgtg cacaactctc agagctttct gtgccagtat ccatggaaga
     4381 acagaatcct ccgtcacttt cctgatgttc ggagagccag gacagtgcaa gatctgctgc
     4441 cgctttcgcc tcgctgtttt cttcgtaata tggtgccagg gcagtcagtg ccatggcagt
     4501 gatatctaca tccatctgcc cggaacccgc tgagagggaa aatccgccgt cactctcctg
     4561 tgcttttaaa agttctgcca ggatctcttc ccggctctgg tatgcatctt ccggcacttc
     4621 ataatccccg gaatccagtg cgatcagtgc ccagatcagt gcatttgttc cctgcgtatc
     4681 gagcggttct gtctttccgc ggttgtacgt tccgtctgcg atcagattga tggtcttctc
     4741 tcctgccatg tcacttactg cctgtggatc accgccaaga gccgcgatca ccagtgccgc
     4801 tctctgatat tcggtcgctt ttccgagttt ttcttttttt gtgccatatt ttttggcgat
     4861 cgctgtttcc atcgcttttc gatagctttt atagtcttcc ttgtatccgc ttctcgctgc
     4921 tgcaaaggca aaccagtcat tgagcgaagt atccgcccct tccagaaagg tcttatcgat
     4981 cagctcgtca gcatttgtga ggctcttggc ccactcaaaa ataccctgga tggtttcttt
     5041 tacttcttct gtcgtgtatt cgtaatgtgt gacagttgct gcactttttg ttgtattttc
     5101 tgctatattt gctgtatttt ctgtcgtaac gtctgattca gaagctgttt gcgtctcact
     5161 gttttgggat gcagactgtg cccgtacttc cttgtttgtg ccggaaacca tcccaacgca
     5221 caggatcagt gccatgagca gggctgtcat tctgtttttt cttttttctg cattttgttt
     5281 ttgatcattt ccctttttca ttaccattca tacccccagt tatctgaacc gccggtcatg
     5341 tcctgcccga tatcagctcc gtatgccagc gtgaaacgca gccgcagtgt atcgccgttc
     5401 tggaaacagt aatcgcttaa gccatagttc gggtaaacgc cgtttacctg atacatccag
     5461 ccgcttcctt ttgtaaaatc ataatctccg aggctgttcg gtgtaaaatc atagatttca
     5521 aagccgtgtt cttccagttt ggtccttaaa tcctccggaa tatttccggt gacaaaattg
     5581 ctctcggaac taatccttgc cagataaaaa ccggtctcat agtttccggt ataatcaaca
     5641 gaatagccat tattctgcag catgcgtact accgtctccg ccgcagtctc tccctcataa
     5701 atatctacat cctgtgacag cagaataccg cttcccacgg tcgttgcttc cacgctgatg
     5761 tggaccgttc cgatccgctc cccgctctct gctgccgtac agaaaatgct gtaagtcagt
     5821 tccgttgtgt tgtcttctgc atcggtgacg gtgatcacca tggtattata gccttgggac
     5881 aattccgtat ggtaactgac atcgccgctg ttctcccaga tcagaccggt aatattgtct
     5941 ccgctgtcac cgaaacagga aatgcggatg ttgtctgcac caagcccgtt tccatgataa
     6001 tcggttgcct gtacctggaa acttaagctc tttcgctgcg tctcaagact gtttccggaa
     6061 tcaccaagcg tacaggtaac tcttggtgcc tccacattgc cttcgccgtc tccgtctccg
     6121 ctgttctgtt tgacataggt tacggtatag gtaatctctt cactgcttcc gtttttttct
     6181 gcatacagcg tgatctggtt ttcgccctct tccagcagca cttcatagtt gtcaccatct
     6241 gtcggtgaaa gctgcgttcc gttcagccag gcggtcagat cttcgacttt gtcactgccc
     6301 ataaaggcct gtgcatagaa gcggtatttc tttttatata cttctttgtc ctccagatcg
     6361 gtgtcaaagc ggatattctg gttctgcggt cgttcataag ttatggtata aacctgctgt
     6421 tcgctctttc cgtctttttc tgcatccaaa acgatctgat tttcgccctc ctgcaaagga
     6481 acgtctttat aatcccagtt ttcatcggca ctgatcnnnn nnnnnntggt atattctttg
     6541 gaaacagtaa atttggtacc gtctttctgc tgataagtga ctgccaccag aatcttattt
     6601 tcgccgtgtg ccagtttcag acttccccgg taagtggtca ctttgccgtc atttaaggtg
     6661 ttttctgttt tcagtacttt cagatccgtc agctgttcaa tctgataact gtaatctgct
     6721 tttgtgaccg tcccgccgtt ttcaatcgtt gtccggaaat actgcctgtc ttttttgccg
     6781 tttttgtctt tgtttttatt tccggagtga ttcttattcg gctttttgtt gtttttatcg
     6841 tctccatccg tcttttcgcc gcctgttccg tctccgtaat tgcctccgtt tgttcctgat
     6901 gcagtcccgc tgctattgcc ggaattgttt ccggaagcat ctctgtttgc ctctgttgaa
     6961 ctgccgtttt cgtttttact ttcgctccct gtgctttctg tgccctggct gtcagctccg
     7021 ctttcttttg acgattcgct ctccgtctcc ttcatatcct gattttctga ctgctgattc
     7081 tgcatctgct gccggctgct tccattctct tttgtctgct ctgccagcac ttctttcgtg
     7141 ccggtcagca tgaccatcga gcttttcttc atgccatcga tcggattttc cggcacactt
     7201 tctctctgcg tacggattcc caggatcatc actcccgaaa acagcacaat aagcagcagt
     7261 gccgcaatgg cacctgctgc ttctgaatgt ttccgcaact ttttcatcat tttttgaaaa
     7321 agctctttca tatgctcctc ctgttattct gaaactgtaa aatattctga tctatttttc
     7381 actgtaatta tgatgttccg gatcagtcac cgcttatttt actttcacag aacgaatttt
     7441 gctgtatttt ccgctgtatt tctttcctcc gacgatctta taggcctgga tcttaaagta
     7501 atattttttc ctggatttca gtttcttcca ggtcagtttt gtagtgttcg cctttttgat
     7561 cgtcttcgcc cgcttccagt ttttcttctt tccgatcgcg taggaaatct catatccgga
     7621 cacgcctttg atcttcttcc acttcagcac tgcttttttg ctcctggatg ccttcaggga
     7681 aataaaggaa acttttggta cggaaacggt gatattctgg tttggatcgg tcggtttcgg
     7741 ctgtacctgc tgcgtttctt taactgtcac tttgatctgt gccaggatct gaggctgctc
     7801 cacgcagtac gctgtgatca gggtctctcc ggatgcaagt gcacggacct gtccgtctgc
     7861 atccacagat gccacctgtg gattagcact gcgataacgg atgctgctcg ctacattcaa
     7921 atcttccggg aggagctgta cttttaacat atgactctcg ccctttttca gcgtcacgct
     7981 gttttctgta aatgtaagtt tctccagcgg aacttccact tttaaggtac aagtcgctgt
     8041 cagcattcca acatgggcgg acaccgccgt ttctccgccg ccgatggctg tcactgtgcc
     8101 gttttccacg gttgccacgg aaggatctgc cacattccag atgatggttt tgtcactgat
     8161 cgtatcttcc ggatcgtaag tcacatgaag tgtcacccgt tctccttttt tcatattgac
     8221 ttctgtttta tccagcgtga tgtttttcag aacaagggac tccagnnnnn nnnnncgcat
     8281 attctgtctc cgcatcggtc aatattttta agatttcttc gctgacatac tgtttctgat
     8341 cttccagaag tgcatcgtac gccgcccttg cttttgcgat agcttcacga ctgttcaggg
     8401 tcacttctcc tatggcattt attttttcaa cagctgactc tgcctggctt ctgttgtatt
     8461 tgagatctgc cagtttattt tctgcctgtg caagcagctc ataatttctg accagatttt
     8521 tctgtgcggt tgtcagtgca gtgtaa
//
LOCUS       DAWDWH010000079         8359 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_4128_length_8359_cov_18.332129, whole genome shotgun sequence.
ACCESSION   DAWDWH010000079 DAWDWH010000000
VERSION     DAWDWH010000079.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8359)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8359)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8359
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4128_length_8359_cov_18.332129"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2097 a         2077 c         1557 g         2628 t
ORIGIN      
        1 tatcaaagtc tttccggttt gtcaagtact tttttcatct ttttgattcc gtccctggat
       61 cgtctggatg attccctgac tgcctgtatc tctcaggccc cgttatttct gcgtttccgc
      121 cgcaacagtt ggtatgatac cactgttgtg cggatctgtc aacaactttt ttaacttttt
      181 ttctaacttt ttctaacagc tctaagtata cttaaaacag acttacattt cactcaaacg
      241 cacacgactc ttgcgaagcg tttatcttag tttcgcctct gcctctgggc ctcatacatc
      301 aggatcgcag atgcaatccc ggcatttaaa gattctactt tcccgcacat tggaatacgg
      361 atacgccagt ctgcactttc tgcaagttct cttgtaagac cattcccctc gtttccgatc
      421 agaaaggctg ttgcttcttt ataattctgt gcatcgtgaa cactgctgcc ctccaaatag
      481 gccgcatagg tttggatctt gcactttttc agtttctgca taaccgcttg catattctca
      541 gcataacaga atggcacgcg gtatatggac cccatggtag cacgtatggt tttgggatta
      601 tagatatcaa cggttccgtt gctcatgata acgcctgtaa tacccgcacc ttctcctgta
      661 cgcacaatgg ttccgagatt gcccggatcc tgaatattct ccagaaccat gatcagcggt
      721 gtactgccct gaatgatatc gtccagatca tactcaaaac gtttcacgag acttaacacg
      781 ccctgtggcg tcttggtatc agaagccgcc tcaaagacgc ggtctgtgac gatttccagg
      841 ctttcccctt tccagatatc ttctttctga tgttttttgt aaaaactttc tgatacatag
      901 gtctttatta cctggtctgt cggtgcttcc cggtacattt tgattccttc tataataaag
      961 attccctgtt cctgacgcag cctgttcttc ttctgaagct ggatcagatt tttgatctgc
     1021 ggatttgcgg tgctggttat catatttttc tcctctgatt gaaaataaaa gagccggtag
     1081 taccttcaca ctggtaatat ccggctctta catacgtttg acttgaatat gggtgttgat
     1141 tatttatatc atgatgccag ggtcaaaagg tctaaatcca catgagcttg ctcataggtg
     1201 gatgaaagac cttgggaccc gccccgatat gagcataaaa gtgggatgat aatcccacga
     1261 tatgcgaata ccggagagga ttgtgggagt gcaacgcacg gaacaatccg taggcatcat
     1321 agttggggac ttttgacacc cacatcatat attaaaacgt cgctcttctg actatataat
     1381 tagatagaaa gggccttgct tacatcatac atatattctg aaatgctctt ctgcattgcc
     1441 agagcctcat caatatcaaa gtctgtaaac tctccgtttc tgtatcctac tacacggttt
     1501 gtctttcctt ctgccagaag gtctactgca taagatccca tcatggatgc atatacacgg
     1561 tctgtacatg tcgggcttcc tccacgctgc atatggccaa ggatggtagc tcttgtctcc
     1621 atgccggtag ctgcttcgat acggcgagcc atgcttgcag aatgtccgat accttctgca
     1681 ttgatgatga tatgatgttt tttgccacgt tttctgtttt cgatgatgtt gttgatcagc
     1741 ttctgctcat cgtaatcata tttctccgga agcaggatat cttctgcacc gtttgcaata
     1801 ccgcagtaca gagcgatgta gcctgcacca cgtcccataa cttcgatgat actgcatctt
     1861 tcatgagatg tggatgtatc acgtacttta tcgattgctt ccattgctgt attgaccgct
     1921 gtatcaaatc cgatcgtata ctcggtgcat gcaatgtcca ggtcgatcgt tcccgggata
     1981 ccgatcgtgt tgatacccag agcagccagt ttctgtgcac ctgcgaagga tccgtcacca
     2041 ccgatgacta ccagtccgtc aataccatgc tttctgcaga cttctgcacc tcttgcctgt
     2101 ccttccggtg tacggaactc tgcacatctt gctgtataca gtacagtacc gccacgggaa
     2161 atgatatcag atacgtcacg tgctgtcata tctttaatct cttcattcag aagaccatta
     2221 tatcccttat agataccttt tactttcagt cctctggaaa gtgcttttct aacaaccgca
     2281 cgaattgccg cattcatacc aggagcatcg ccgccgcttg taagtacgcc tattgtcttt
     2341 atttcttttg ccatcttgaa acctcctcag attcgctgtt tagactcttt gattttccta
     2401 accaaatact tcgctctcaa ttttaattca ttttaccgtt attttcaata cacttttcaa
     2461 caacttttac attgttctgc ccatatcttt caacaatttt ctgtaaaagt gtctcatccg
     2521 cacatatgga ccatgcttta cccagtcttt tcatagattt cggggaacga atgtacacta
     2581 caacctgatc cgttccatcg gatgaacgga ggatctcata taacgctttc tcttctgcca
     2641 taaaactctc cctatccgaa aactggatcc agagttcttt tggaaggtct tcaaacttcc
     2701 agatcttatc gcagatcagt ttgcttgcct tttcttcttc tgcagatacg tgtccctgga
     2761 taaaaagttt gccatcctgt gtaatgctgt ttcggtaaat ttcatactgt cttggaaaaa
     2821 ctacaacttc cactgtgcca agaagatcct cgatggtcaa aaagaccatg gtcttattgt
     2881 tttttgtatg cttgatcgta atgccttcga ccatgcctcc gatgatctcc tctgctccat
     2941 caaacaggcg ggagccgtgc agttcttcct gataggcaaa atccatggta gttgccgaaa
     3001 tatttttctg ccacatcttt tcatattttt ccagaggatg cccgctgata tacacgccaa
     3061 gcacttcttt ttcaaaggca aggagctgct ctttatcata ttcttctaca tccggcatgc
     3121 ggatttcaaa atctttttta tcttcctccg ccacaaagtc aaacagagac atctgacctg
     3181 ccattgcatt tttcttctcc tgatttacac tgtccacaac ctgcacatag atcatcatca
     3241 gctgttttcg gttatcacaa agaccatcca gtgctcctgc tttgatgaaa ttctctatgg
     3301 tgcgtttatt taattctttt ccggacagac gttccacaaa attgcggaaa tttcgataga
     3361 ttccattctg gtttcgttcc cgcacgattg catcgatcac cggttttccg atacttttga
     3421 tcgcagaaag gccatatcgg atgcttttat tatatttaaa tgtttcttct cctgtttttt
     3481 ccgtgctttc gttttcttct acggaaaaag cactctcgcc cttgttgata tccggcggaa
     3541 gcagacggat ccccatctgg cggcagttca gaatatattc tgccactttc ggtggattat
     3601 caatgacaga ggtcatcaaa gctgccataa attctaccgg atagtaatat ttcagccatg
     3661 ctgtctgata tgcaactacc gcataacatg ccgcatgtga tttattgaat gcatattttg
     3721 caaaatcggt catctcatca tagatatgat ttgcaatttc ttctgaaatt ccgtttttga
     3781 tgcagcccgg cacgccttct tcttcatttc catagacaaa attctgacgt tctttttcca
     3841 tgacggctac ttttttcttg gacatcgcac gacgcagaag gtcgcttcgt ccaagcgtat
     3901 agccgccgag ggcacgcacg atctgcataa cctgttcctg gtatacgata cagccgtaag
     3961 tcggctccag gatcggctcc agttccgggc attcgtacgt gatcgtatcc gggttatttt
     4021 tacctttgat ataacgcgga ataaaatcca taggacccgg acggtacagg gaaattccgg
     4081 cgatgatatc ctccaggctc tgcggtttca gctctttcat gaagtttttc attccgccgc
     4141 tttcaagctg gaaaatgcct tctgtcttac cagttccgat ggaatccagg acggctttgt
     4201 cattaaaatc aatatgatcg atatcaatga tttttccggt cgatttttct gcaagacgga
     4261 ctgcattctg gataacggta agggtacgca atcccaggaa gtccattttc agcagtccca
     4321 attcttccag tgtcgtcatc gtaaactgcg tcgtgatcgc accgtcgctg cctctggaga
     4381 gcggcacaaa ctcatcgacc gctttctgac agatcacaac cccggctgca tgcatggagg
     4441 tatgacgcgg cagaccttcc agtcttctcg acatatcgat cagatcatgc acctgcggat
     4501 cttcgtcata gatttttttg aattccggat ttctctgaaa tgctttatct atagtaatgc
     4561 cgatctcagt cgggatcatt tttgcgattg aatcgaccat tgcatacgga agatccatta
     4621 cacggccgac atcacggatc acgcctcttg ccgccatcgt accaaaggtt acgatctgtg
     4681 caacctgatc tttgccgtat ttttccacaa cgtaatcaat gacttcctgt cttctttcga
     4741 agcagaaatc cacgtcaata tcgggcatgg agacacgttc cgggttcaga aaacgttcaa
     4801 acagtaagtt atatttcaga ggatcaagct gtgtaatgcc aagtgtatag gagacaaggc
     4861 ttcctgcagc cgagcctcgt cccggtccta ccataatatc gtggtcgcgg gcaaatttga
     4921 tataatccca tacgatcagg aaataatcga catatcccat tttctgaatg atgccaagct
     4981 catagtccag acgttctttt aatcctttcg tctcttcgcc ataacagcga accagtccgt
     5041 cgtggcaaag tttctgtaaa tattcccatg atgtatagcc atccggcaca tcatatttcg
     5101 gaagttttgt cacaccaaac tcaatctcca catgacaacg gtctgcgatt ttctgtgtgt
     5161 tttccagtgc ttctagtgca tacggaaaca ggtttttcat ttcttcttcg gattttacaa
     5221 aatactgtcc gccttcataa cgcatacggt cggtatccga cagtttcttt ccggtctgta
     5281 aacacagcag aatatcatga gaatccgcat cggatgcata cgtgtaatgc acatcatttg
     5341 tcgccaccag atctatgcct gtctctttgc tcatccgaag gagctgcgta ttgactgctt
     5401 tctgttccgg gatgccatga tcctgaagct ccaggaaaaa gttgcccctt ccgaaaattc
     5461 cttcgtagcg tctggcggca cgcactgctt cttcgtacat accacgcacc agatattttt
     5521 ggacttctcc tgccagacac gcagacaggg caatgatacc ttcatgatat ttttccagga
     5581 cttccatatc cacacgcggt ttataataga aaccttctgt aaaacctttt gatacaatct
     5641 tggtcagatt actgtaacct gtgttatttt ctgttaataa taccagatga tagtagcggt
     5701 catcaccgct tccggcttct cggtcaaatc tggatccggg tgctacatac acctcacatc
     5761 caagaatcgg attgatcccg gctgcttttg cttcacggta aaaatcgatc acaccgtaca
     5821 taacaccgtg gtctgtgatg gccgcactgt tcatgcccag ttcttttacc cgtgcaacgt
     5881 attctttgat tttatttgat ccatccagca aactatattc tgtatgaaca tgtaaatgtg
     5941 taaaactcaa actgcacctc accctctcaa tatcatgtag atcactacta taactgttgc
     6001 caccgcaaat gccggtgtaa acatggtttt cggttcaccc ggacgttttt ctcttcgttt
     6061 aaaacaccat ttcagatgct cataacgtcc acagtgtttc gcaatccaga tcagcactcc
     6121 gatcgctgca aaaatacaga tcgccgcgat cacggaataa acattgatca caccgatcat
     6181 gtcctgtgtt gtcaatgctc cggaacttgt agaagaactg ccggcagaaa ttccataaag
     6241 cagtttggaa actgccatca atgccgtact ccatccattg atcacaaaat gtgctgtcat
     6301 cgtagcaaaa atacttcctg tcgcctccag cagaatgcca aatgcaatgc ccagtacaaa
     6361 agcatagcag aactgattaa agttcatgtg catcagacca aacgccagtg cagatccaag
     6421 aatcgcacac cacacacctc tctgacgata tccattataa tagagaccac ggaacgtaaa
     6481 ttcttcgcag atcggcggaa tgactgccat cacaatcata ttgacccaga atgcattctg
     6541 cgtcagttcc gactgtgaac tttcaaatgc atttcttacg aaaagcatag aaagcagatt
     6601 cagccaggat gtaacaggga tcatcagcac tgcatataaa atgaccattg cgatcaccga
     6661 taacttcagt ggtttaaatg gcatccactt tctcggatct accttgttta tgatcataaa
     6721 tccaataacc ggcatcaaat acagaagctg gctcaaaagc atcagctgcg tcgcatccat
     6781 cgaacgaaca aacgggataa atcccagaaa aatcccggaa agcagtatcc agataacggt
     6841 caccagaaac agcctgttta tcttcggtgt cttgttcatt tgtttttcct ccacgttttt
     6901 tatttttaat aatgtcagtg aaatgtcctg tcttgccggc aagaacgaca atttgacatt
     6961 ttgatcctgg catcataatt ttattctacc ataaaatcca tttctttttg cattttcaaa
     7021 gtttaatttt taaaaacaaa gataaaaaaa cagccgggca ttgcccgact gccttgattt
     7081 tgatttttac ttattgttat acaatcagtt attgcggctg atcatgtaat ccatcagttt
     7141 atcaatatct tctttttcgt atgcaacgat ctccagttcc ggaatttcgc cgtttgagaa
     7201 gatatttgcc agagatacat actgtgacag cttagatttc agattcagtc tgtcaccttc
     7261 gcctgttaca agctctactt ttcctttgca gctgtctact acctcaaaaa atttatcaat
     7321 atctttaatt cccattactt tcataattca ttctcctttc agataaaaat gttgaaaagt
     7381 gattattcta tttatttgat tgcgattgcc tcgatctcga tcaggacatc ttttggcagt
     7441 ctcgcaactt caacacagga acgtgccggg taggtaccgg tgaaatattt tgcataaact
     7501 tcattgatgg taccaaagtc ttccatattt ttaataaata cggttgtctt taccacgttt
     7561 tctgtggaag taccggcttc ttttaacagt gctgccagat tgccgatcgc ctgatctgcc
     7621 tgtgcggctg caccttccgg aatctcgcct gttgacggaa ctaccgggat cactccggat
     7681 gtatatacca taccgtttac ttcgatcgcc tgtgaatatg gtccaattgc tgcaggagct
     7741 ttgtctgtac tgattgcttt cttcatgctc atgccctcct aacaaatgta tgctctttgc
     7801 tgataaagat gtttctatct tttttcattt cctattataa attcagtttt caaacgacgc
     7861 aagcaaattt ttcgtgaaaa aatcgcagga aaatatagca taatgtttaa tgcctatctt
     7921 caaattgttt gattctcaaa aaattattgt gcgtttttac cgttttatga tgcgattctc
     7981 tgtaatttcg atacgtccct gtttcaacag tcttccaacg gcacgtttga atgcattctt
     8041 actgagtccg aattctcttt tgatgatctc cggtgaaact ttatcgttaa acggaagtac
     8101 gccttcaaat tcttcaatca ccttcatcac ggcttctgca tctttttcca tctgctgcgg
     8161 aatcttttct ttatccgaaa gatccagttt gccatcttcc tgtactctcg tcacacgaag
     8221 atttaaaata tcgcccacgc ggtatttgcc ctgtgcttct tttgcaggga tcagaccgga
     8281 ataacagtca tccactgcca caaaaacacc aaaattctcg ctgatctcat aaatacggcc
     8341 ggtcacttta tcgtctctc
//
LOCUS       DAWDWH010000080         8306 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_4155_length_8306_cov_12.046903, whole genome shotgun sequence.
ACCESSION   DAWDWH010000080 DAWDWH010000000
VERSION     DAWDWH010000080.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8306)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8306
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4155_length_8306_cov_12.046903"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3717..3726
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7784..7889
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2313 a         1634 c         2313 g         1930 t
ORIGIN      
        1 tccacttatc tttatgtaca taaacggatt gcacagcgtg gttacaggac ggaactgaca
       61 agcgggatca cttcattttg tgcagtagcg gcaaagctca atatcggtct tgtagaaaag
      121 gcagaagagc ttcatgtgat accggcatcc tatcagatcg aagatgccct gaaattgccc
      181 ggaacaaaag tcctgatgaa agcaggaaga aagatcggac aggtgaaaga gcttctgaaa
      241 aaagccggta tgcaggcgat gatgatcgaa aactgcggta tggaaaatga aaaaatttat
      301 caaacggtgg atgagattcc ggaaaatgcc ggatattatt cgcttattat cgtaaaggag
      361 aattagtatg atacattttg tcggagccgg ttccggagcg gcagatctga tcactgttcg
      421 aggcagaaaa tttctggaag aagcggacgt gatcatctat gccggttctc tggtcaatcc
      481 ggagctttta aattatgcaa aagaggcgtg caagatttat gacagtgcaa agatgacgct
      541 ggaggaagta ttggatgtga tgcgtcaggc agaaaaagac ggacagacga cggtgcgtct
      601 gcataccgga gatccgtgca tttacggtgc aattcgtgag cagatggatg tactggatgc
      661 agaaaatatt tcttatgatt attgcccagg tgtcagttcc ttctgtgggg cagcctctgc
      721 tttaaatatg gaatacacgc ttccgggtgt atcacagagc gtgatcatca cacgcatggc
      781 aggccgtaca ccggtgccgg aaaaagaaga gatcgcttcc tttgcggcac atcaggcaac
      841 gatggtaatc tttttaagca ctggtatggc agaaaaatta tccaagcgtc tgattgcagg
      901 aggttattcg gaaaatacgc cggcagcgat tgtctataaa gcaacctggg aagatgagaa
      961 agcggtgatc tgcaccgtag gaacattagc aaaggcagca gaagaaaaca acatcacgaa
     1021 gactgcactg atgatcatcg gagatgcggt aacgcacagc cattatgaac gttcggagct
     1081 ttacaatccg gcctttacaa cagaatttcg tcaggcaacg aaagagagat gacaggaaaa
     1141 tgggacagaa acttgggatc gtcagtttta cggagcatgg cagtcagtta aatgagcggc
     1201 tgcgggaaat gctggagcgt tccggatatg tatgcgagtc ctatgcagcg gaaaaatatg
     1261 caggaaaata tcatctgttt ccactgaagg tttccgcaaa agcgtggaca gaaaaaatgt
     1321 tccaaagcat ggatgctttg ctttttatcg gtgcgtgcgg cattgcggtg cggagcatag
     1381 caccttatct tcagggaaaa gacaaagatc cggccgttgt tgttctcgat gagaggggga
     1441 tttttgcgat ttccctgctt tccgggcatc tgggcggggc aaatgagctg acagcggtgc
     1501 tcgcaaatct gaccggtgcg attccggtca ttacgacagc aaccgatatc aacggacgtt
     1561 ttgcggtcga tgtgtttgca aaaaagcaga atctgtggat ctcagatctg aaagcggcaa
     1621 aagcagtttc tgcggcggtg ctcgatgaag agccggtcgg atttttcagt gaatttccgg
     1681 tgacaggcga aatcccatgc gaactgcaaa gacttgcgga tggagagcga tttgagggaa
     1741 actgcggtat ggtgctttcc ttgaacgaag aaaagaaccc gtttgccgtg acgctgcacc
     1801 tgatcccacg gatcgtaagc cttgggatcg gctgcaaaaa aggtacaccg caggagacgg
     1861 tcgagcagga agttttaaaa gcactgcaaa gctgtcatgt gtcgatacac agcatttttg
     1921 cggcagccag cattgatctg aaaaaagaag aagcgggaat ccttgcgttc tgtgaaaagc
     1981 atgcgattcc attttgtact tatacgaaag aagaactgat ggatctcgaa ggagatttca
     2041 cggcatcagc atttgtaaaa gagacaaccg gtgtggactg tgtctgtgaa cgaagtgcca
     2101 ttcttgcagg aggcggtgca gagctgcttc tgaaaaagcg ggcggcaaac ggcgtgaccg
     2161 tcgcactggc tctcaaagat tggagtgtgg attttggata ataagatata tgtggtagga
     2221 attggaccag gagcttatga gcagatgacg atgcgggcgg cagaagcact tcgcaggtgt
     2281 gatgtgatcg tgggctatac cgtttatgtg gagcttgtaa aggaacattt tgcaaacaaa
     2341 cgtttcctga caacgccgat gaaaaaagag atcgaacgct gccgtctggc atttgaagaa
     2401 gcaaacaaag gttataccgt tgcgatgatc tgcagcggcg atgccggagt ctatggcatg
     2461 tccggtctga tgctggagat cggtcaggag tatccgcatg tggaagtgga agtgatcccg
     2521 ggtgtgacgg cggcaaatgg cggggcagct gtgcttggag caccattgat ccacgatttc
     2581 gcagtgatca gtttaagtga tctgctgaca ccgtgggaaa agattgaaaa acgtttgctc
     2641 tgtgcaagcg aagcggattt tgtgatttgc ctttacaatc catccagcaa aaaacgtcac
     2701 gactatctgc agaaggcctg tgatctgatg atgagaagca aagatccgga taccgtgtgc
     2761 ggaacggttt ccaatatcgg gcgtgaggga gaaacgtttc gtgtgatgac gttaaaagag
     2821 ctgcgggata ccagggtgga tatgtttaca acggtttata tcggaaattc tcagaccaaa
     2881 atgctgaatg ggaaaatggt cacaccaaga ggttatatcc atggataaga ttttattgtt
     2941 tgccgggacg acagaagggc gaaatctggc agaatttctg gagaaaaatc agataccgac
     3001 ggaagtttgt gtggcaacgc agtatggaga gacgctctta gaagaaggga agtatcttca
     3061 tgtccacgcc ggaagactgg acgaaacaga gatggagcag cagatccaaa agcagcagat
     3121 cacgctggtc atagatgcga cgcatccgta tgcggtgatc gtatctcaaa atatccgcag
     3181 ggcatgcagc cggacgggaa cagagtacat ccgtcttgcg agaaaagaga cggacgcttc
     3241 ctggaaacag gagatggagg atgtcacaga agttgcaagc gtggcagaag cggcagcttt
     3301 tcttgcaaaa aaagaggggc ggatctttgc ggcgaccgga agcaaagagc tttctgccta
     3361 tcaggtgatc ccggattatc aggaccgggt ggtggcaaga gtgctttcca ccccggaggc
     3421 agtatcggag tgtgccatgc tgggattttc cgggaagaat ctgatctgta tgcagggacc
     3481 gtttacggaa gatctgaatg tggcgatgct caggcaggca caggcttcat ggatggtgac
     3541 aaaagaatcc ggaaaagccg gaggttttct ggaaaagttg cgtgcggcaa aaaaagcagg
     3601 agcaaagctg gtcgtcataa aacgaccggg ggagcgatcc gaagaaattg cggaagatca
     3661 aaaggaagaa aatttatatg caatatgtga tgaaggacag atccgaagcc ttcttgnnnn
     3721 nnnnnntctt ggcaaacggc ttggcatttg tccgaaacga cagctgtatc tggtcgggat
     3781 tggcatggga aatgaaaaga accgcaccgt ggaggcagaa cagatctgtc agtcggcgga
     3841 tcttctgatc ggtgccagaa gaatgctgca atctgtgaaa acagaaggaa aggcagtctt
     3901 tgaatcttat aaaccgtacg agattgccgc ttatctggca gaacatccgc agtatgagac
     3961 ggcagcggta ctcttatccg gagatatcgg tttttacagc ggagcaaaaa aactgtacga
     4021 tgcgattgaa cgcacggaag gactggaaca gatggaaatt tacccggtca gcggcatttc
     4081 ttcggtggtc tatttctgcg gaaaactggg cgtttcctgg gaggatgtcc atcttttgag
     4141 cctgcatggc agaaaacaga atctgatcga tgcagtcaaa tatcatgaga aagtatttgt
     4201 attatgtggt gaaaaagacg gaattcccaa aatctgctgt aaactgaaag aatatggtct
     4261 tggcgatgtg aaggttgcag tcgggactga tctgtcctat gaacaggagc ggatcgtaca
     4321 gggaacagca gaaagcctta tgaaagagga ttttgcaccg cttagtgtgc ttttgatctg
     4381 caatccggat gtgaaaaaac gcattggtca cggactggat gacgatctgt ttctgcgtgg
     4441 gaaagtcccg atgacaaaaa gcgaagtgcg gagcatttcg ctgtcaaaat taagactgca
     4501 caaagatgct gttgtctggg atgtcggagc cggaaccggt tcggtttcca ttgaggcggc
     4561 aagtcttgca aaagacggcg ttgtctatgc gattgaaaag aaagacgaag ccattgatct
     4621 gctggagcag aataaacgaa aattcggaac agacaatctt gagatcatca aaggattggc
     4681 tccggaggca ttagaggggc tgccggcacc gacacatgct tttataggag gttcttctgg
     4741 aaatttaaaa gaaatcctgg aagttttgct ggaacagaac ccaagggtgc gtgttgtcat
     4801 caatgcgatt gccctggaga cggtagcaga agcgatgcag tgcctgaaga gcatggcgtt
     4861 taccgatgtg gatatcgcac aggtatcggt ggcaaaggga aagaaactgg gctcttatga
     4921 gatgatgatg gggcagaatc cggtttatat tttctcctgc acaggaggtg aaacagcatg
     4981 aaacagccaa gatttatgct tgcagcaccg tcaagcggaa gcggaaagac gatgatcacc
     5041 tgcgggatat tgcaaatatt aaaaaacaga ggatatcgtg tctcttcctt taaatgcgga
     5101 ccggattaca ttgatccgat gtttcacaca aaagtgctcg gtaccatctc caggaatctg
     5161 gatacttttt ttaccggaga agagatgaca cgctgtctgt ttgcaaaaac ggcacagcag
     5221 accgatcttt ccgtgatcga gggtgtgatg ggctattacg acggacttgg cggcatccgc
     5281 acaaaggcaa gttcctatga tcttgccagg gtcaccgaca cgccggtcat tctgatcgtc
     5341 aatgccaaag gcatgagcct gtccatcctt gcccagatca aaggctttct ggattatcag
     5401 aaagacagta aaataaaagg cgtgatctta aatcagactt ctgagatgac ctgccgcatg
     5461 ctgacaccga agatcgaatc ggaactggga atctgtgtgt ttggatatgt cccgaaaatt
     5521 gcagactggc atctggagag tcgtcatctc gggctggtac tcccggacga gatcagcgat
     5581 ttaagagagc agatgcagcg tctggcggat attctggaga aaacgctgga tattgaaagc
     5641 attttgcaga tggcggaagg tgcgaaagag atggaggacg atatgccgaa atccctgaaa
     5701 cagctttttg cagatccgca cgttcagaag atacgcactc agagaccaca gatcgcagtg
     5761 gcaaaagatg aggcattctg ctttctgtat gaagacaacc tgaagctttt ggaagaactg
     5821 ggggcagaga tcacattctt ttcgccgctc cataatgcga aagtgccgga aaacgcagat
     5881 ggacttttgt tgccgggagg ttatccggaa ttgtttgcag cagggctttc cgaaaacagc
     5941 gaaatgcttg catcgatccg ctcctgtgaa aaaaaagcga ttccgatcct ggcagagtgc
     6001 ggcggtttta tgtaccttca tcaggaaatg caggacatgg aaggaaagac atatccgatg
     6061 gcaggcgtga taaacgcagt tgcgtaccgc accgaaaagc tgggaagatt tggatatatc
     6121 acactggatc tgaacgaaga acaggcagag gcaagaaaca tatggcagca gggatttccg
     6181 gtgcgtgccc atgaattcca ctattttgac agcaccgatc cgggaagtgc ctgtctggca
     6241 aaaaaaccgg ccgggaaaag aagctggagc tgcagttatg caaaagatgg cagtctgatg
     6301 ggatttccgc acctatatta ttattccaat ccgatgttcg catttcggtt cctggaaaat
     6361 tgtatggcaa aaaaactgga aagggaaaaa gtatcatgat atatggaaga gaagggatga
     6421 tggcactttt gctcggtgtg tttcttgatc tgttgatcgg tgatccgcac tgcttttatc
     6481 atccgatccg gctggtcggc aatctgatct ccgttacgga aaaagggctt cgggcggttt
     6541 ttccaaagac aggacagggc gaaaaaacag ccggtgtatt tctgtcagtg atcgtctgca
     6601 gcgtaacagc cggagcggtg gcagctgtgc tgtatctggc cggaaaaatc catccgtatc
     6661 tgcatctggc tgtgatggca ttgttctgct actggatgct ggcggcaaaa tctctgaaag
     6721 atgagagtat gaaggtttat agagaactga aaaaagaaga tttaaaggca gcacgccgtg
     6781 ctgtttccat gattgttggc agagatacgg atcagctgac ggcagaaggt gtaacaaaag
     6841 cagcagtaga gacagtggca gaaaatgcct cagatggagt gatggcaccg cttctgtatc
     6901 tggcagttgg cgggcctgta ctcggagctt tttataaggc agtcaacacg atggattcta
     6961 tggtcggata taagaatgag aaatatctgt atttcggtcg ttttgcagca aaggtggacg
     7021 atgtactcaa ttacattccg gcaagactgt ctgctctttt tatggtggcg gcagcaggac
     7081 ttaccggtct gtcagcaaaa aatgcctgga agatcttcaa acgagaccgc tataaccatg
     7141 ccagcccgaa ttcggcacag acggaggcag ccatggcagg ggcactcggc gtgcagcttg
     7201 ccggagatgc ctggtatttt ggaactttat ataaaaagaa aacgatcgga gatgcactgc
     7261 gtcccgtgga atgtgaggat atcagaagag caaacaggct gatgtatgcg tctgcagggc
     7321 tgcttcttat cgttgttatg cttctttatt gcagctttaa catactcaca ttgttatgat
     7381 gccaggaagg gaacatacca tgaacaacaa gcgtcacaaa catggcggag atatttattc
     7441 agaggcctgc actctggatt attccgcaaa tatcaatcca ctcggaccgc cctcttctgt
     7501 aattgaggcg gtttatcgga gcatggaaca gattgtaaat tatccggatg tacagtgccg
     7561 gaagctctgc catgcgatcg cagaacatga atatgtaaaa gaagagcagg tgatctgtac
     7621 aaatggtgcg tctgagctgc tctatgcttt tgcagcggcc ttaaaaccga aaaaagcact
     7681 gcttctggag ccgggatttg cggagtatga ggctgcactt tcggcagtcg gatgcgagat
     7741 tttttattat cctttggggg aagagaaggg ctatgcactg gatnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna agcactttca gatgatctgg atatgatcat
     7921 tttgtgcaat ccaaacaatc ctaccggtgt tctgatacag cagccgcttc tggtgcggat
     7981 cgcagagaaa tgtgaaaagt atgggataca ttttctgctg gatgaatgtt tccacgaatt
     8041 tctgatggca ccggacagtt acagcatgcg aacgtttctg gagcgttttc cgcatctgtt
     8101 tcttgtggat gcctttacga aaatttatgc cataccgggg ctgcgtcttg gctatggttt
     8161 aagtgcagat gcagatctta tggaaaaaat ccgggatgtc atacagccat ggagcgtttc
     8221 cataccggca caggaggcag gtattgcggc actttcggaa gatacttacg tacataatac
     8281 acgctggctg atccgggaag aacgca
//
LOCUS       DAWDWH010000081         8098 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_4257_length_8098_cov_11.216835, whole genome shotgun sequence.
ACCESSION   DAWDWH010000081 DAWDWH010000000
VERSION     DAWDWH010000081.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8098)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8098)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8098
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4257_length_8098_cov_11.216835"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2998..3007
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1978 a         2057 c         1634 g         2419 t
ORIGIN      
        1 tcgagaatag aataaccggt cttcagtaag aagttcgtgt atttgaacac gaccagaatc
       61 ccgatattga gcaccagccc ccagatcagg atccgttttc gctgtttttt gttgtttgcc
      121 ttcatccgct tgatctgttt gcggtcaagg ccttcttcct gtttctgttt ttcaagtttt
      181 tcatgcaatt tccccatctt gcatgccaca acataagata ctgcggttgc cagaagcagc
      241 accagaaact ggtcaaagcc tgctgccaga tagaagatca catttgcgac aagcagcaca
      301 tacatacggt gccggggtct tatgatataa taaatacaga atactgccac aagaaagagc
      361 agatatgttg atgatgtgta cgacatgatt ttccccttat taaccttcct gatgcagtgc
      421 tttgttaaac atatcccaca tctgtccata gccttccgat gccggtgtga ccggtttcgg
      481 aatcgtattt ggcacatcca tcttgatacc gccctgcttt gccatgacca agatccttgt
      541 tcccaggcag actgcctcct ggatattgtg cgtgataaag ataacggtta ctttttcttt
      601 ctggtgcagc ttcatcagct cttcctgcag atgatttcgc gtcatggcat ccagactggc
      661 gaacggctca tccatcagaa tgatctgcgg cttgagtgcc agtgctttgg cgatcgccac
      721 tctctgtttc ataccgccgg aaagctgatg cggatagagc gttcttgaag aaagcaaatc
      781 gactttctca agtgccgcat cggaaatctc ccgcaatttt gttttatctt ttaaaccctg
      841 tattttcaga gggtactgca catttttttc tactgttttc cacggaagca gctgattaaa
      901 gtcctgaaaa accatgatgc ggtccgtccc aggctcgctt acctcttttc cgtttacttt
      961 gaccgttccg tcaaaggttt caaaaccgcc gatgcagcga agaaatgtag tctttccaca
     1021 tccggacggt cccaggatgc agacaaattc gccctcattc atcgtaaagt ttacgttttt
     1081 gaggacttca tttccgtccc cttttccacc attgtcatag atttttgtca ggttctgcac
     1141 atccaaaacg ggagttttcc ccttctgctc ctgtgtcatc tgctcatccc ccatttctta
     1201 agtgtcattt tttctatggt gtggaaaact ccgtattcta ctacaattcc gattacaatg
     1261 atcaccagaa gtgctgcaaa aacaccggcg atatccacat tggtacgctt catgaaaata
     1321 aaccatccga taccggctcc ggaactggtc gttccgaaga tcatctccgc actgatcaga
     1381 cctctccacg ctctcgccca tccggtgcgg ataccggaaa gaatatagga gaacgatgcc
     1441 ggaatataga tgccggtcac aagtccgagt tttccaagtc ccatattttc cccggattct
     1501 atgtagatct gcggcaccgc ccgaaatccg tccataatgc ttctggacat tggccagatg
     1561 atcgaatgta ccacgatcag gatgatcgtc ccttccccga tccccatcca caggattgcc
     1621 agcggcagca gtgccacacc aggtatcagg tcacacaggg aaacgaccat atggtaaatg
     1681 gaataaaacg tcttgctgac gatggacagt ccggagagga taaatgccag cacgattccg
     1741 atcagcagcc cttttgcaat caggcttaag gaataaagcg tcatcgctcc cagcccgtcc
     1801 cgcgaaaatc ctttgatcag cgactgaaat atctttccaa gtgacggaaa catcagttcc
     1861 ggaaaggaac ccatctgata gacgacctgc cataccacca gaacagcaac aataaaaaca
     1921 atctgatttc ttaaattttt catcgttctc atccttattc tttcttttcc gacagatctt
     1981 atcttgccga ctctgttgtg attgcttcaa aggattccgg tgccttgtct gcaaaatcat
     2041 tctccgccat aaagtttgcc atatccatga cgcccggaag cttcatatca tagacacatc
     2101 ccggatcctg catccagctc agcatcattt ctgccgtgac atcttctttt tggcagagaa
     2161 tatttgctgc ttcttcctgg ttgttgttga tgtaatccat cgcttcctgc gttgcgtcta
     2221 caactgcctg ataaacttct ggctgatttt catacaggtc atcagatacc agaccaacga
     2281 taaaggcatt tccttccggc catacggaat ccaggctctc agcttctttg atattatcct
     2341 gttcttttgc cttgaaaata taaggtgctg ttgtaagctg acatgccaca ctgccggaca
     2401 tcagggcagt catgccgtcc ggatgggaca ttgccaccag attgttgtcc agtgcatggg
     2461 catctccaag ttcttccttt gcgagcattg ccagcagaat atgctgaata cttccgatat
     2521 ttacaacgga aatcttttta tcatctgtga tatcttttaa cgaatggatc gaatcatcgt
     2581 ttgtaagcag ttcatgtggc tgtgctgcaa tcgttccgta cattttgtaa ggaatgcctg
     2641 ctgtgacgcc ggaaataaac ggtgcaattc ccatacatgc cacatcaata tccccggatg
     2701 ccatcccttc attgatcgcc gatccggaat ttaagctgac ccactcaacc tcgatatctc
     2761 catcgtagtt tttctcgatc agtccctgtt cctgcatgat ggtcagcggt gcataagcca
     2821 gtccgtactg ataagcaatc tttacttttg ctgtgtcccc actatcctgc atacttttct
     2881 gactttttcc gcatccgctt acccccaaaa ccatggatac cgccagaatc attgctgtta
     2941 ttttcctcac cctttttttc atcgtttttt cctttctttt ggccacccgg tctactgnnn
     3001 nnnnnnncta ctgcctggtg catttttcat ttaagccacg gacatgataa tatcataaat
     3061 tgctctattc tttcaacaca tgataatgtc cacgaattgc tccgttataa aataactctc
     3121 gcaattcttt caacattatc ataacatatc cggggcattt ttcatcagct tattctataa
     3181 ttgtgatata attatgacat tttcattaca tacagcactg ttttcgtcat tttttcataa
     3241 acttcctggt caggaatctg gacaaagcgg catcggaagg caaaaaaatc ccccggaaat
     3301 tgctctccgg aggattgtat gttctgcttt atgctctggc tgccgccttt gccttcgctt
     3361 tctcacccag aaaatcaatg gatgtcttgc agattacctt atcaaaaata atgaacatcg
     3421 ccggcaggat gaagattacg catgccatac tgatcagggc acctcttgat aacagtgtac
     3481 agattgcacc gatcatatca actttggaat accatgccac accgaacgtt gctgcgaaga
     3541 aactgaaacc acttgtcatg atagacagtg cacatgcttt gtgtgcattc atgacggctt
     3601 ctttcttgcc aaatcctttt cttctttcct tctgataacg gctcgtcata aggatcgcat
     3661 agtctacggt tgcaccaagc tgaatggttc cgactacgat acttgcaacg aatggaagtt
     3721 cgattccctg gaaaaacggg attgccatgt tgacgttgat ggcaaattca ataaccgcaa
     3781 ccagaatgat cggaacggaa atggatttga ataccagcat aatgatgatg aagatggctg
     3841 ctacggaagc aacgtttaca tttaccaggt cgatgtccgt tacatcctgc agatctttca
     3901 tcagcggagc ttcaccgatc accataccgt ttgaatcgta tttctttaca agatcgttga
     3961 tcgctgcgat ctgactgtta gattcgtctg ttgcggagct gtactttgag cagacaaata
     4021 ccagttcctg ttcatcactt tgcagcatat ctttgacatc tttcgggatc atggaagccg
     4081 gcacgtttgc accgaatacg ctgttcagtc caaatgccca tttcacaccg tccacctttt
     4141 cgatgtcttt catcagcgta gtcttgtttg cattggaaat gtttttgtcc atcatgatga
     4201 tgtgcatggt gctcatgtcg aaggtatctt ccagtttttt gtttgccaca tttgaatcca
     4261 gtgttgacgg aagcgattta tcaatattgt agtaaatctt ggtgtggttg tttccgtata
     4321 ctgccggaag cagtccgatg cagaaaacgg ccagccagat tttataatgc ctgatgataa
     4381 aaccggacag tttgttcgta ttcttgatca gcggtttatg ttttgtcttt tcgatcagcg
     4441 gatcaaagat caggatgatc gccggaagca gggttacaca gcagataaca ccgatgataa
     4501 caccttttgc cattacgata ccaaggtttt taccaagtgc gaatgtcatc agacacaggg
     4561 ctgcgaaacc ggctaccgtt gtgatggaac tggatacgat ggatttgaag gtattggata
     4621 ttgcatgacc catcgctctg ttcttgtcgc cagggaatcg ttttttattc tcctcgaaac
     4681 tgtccagcag gaagatcgag tagtccatcg taacaccaag ctgaaggatt gctaccagtg
     4741 cctgtgtcag atacgaaatc tcacccagga aaagattcgt tcccatgtta tacaggatcg
     4801 agattccgat acagagcagg aacaggatcg gcaccacaaa ggatgttccg gtaatctcca
     4861 gcacgatgaa agaaaacagt gctgcgatcg ccacgtagat cggcagttcc tgcagacaca
     4921 gattcttgat atcggttacg acaccggaca taccgctgat aaagcactgc ttatctgcaa
     4981 tggcacgcat gtttgtgaca gcttccatcg cctcgtccga agatgtggtg ttatcaaaaa
     5041 gtaccagcat cattgtagaa tcgtccgcaa agaagatatc ctttaaatct tttggcatca
     5101 tgcttgtcgg aacgctgata tcagcgatgt catcatacca gagtaccttt ttgacatgtt
     5161 cgatctcttc gaacttgttt ttcagtttct gcacatcttt aagcggcata tcctcgacga
     5221 cgaccatcga gaatgcacct gtgccgaatt catccaccat aacatcctga ccttttaccg
     5281 tttccagaga ttccggaagg taacttaaga tatcatagtt gattcttgtt tttatggttc
     5341 caataacaga tggaatgaga agcaaaattc cgatcagtac gatcaggata cggtgttttg
     5401 ctatccattt tccagtcttg accattgttg tcatctccta tctttcctta atatttatga
     5461 taccggggag caaagatttt attcccctga catcccgttt acggattctg tgtcggctga
     5521 tcattctgtg catggattgc ctccagtgac atcagcagtg cataatttga tgcctgcatc
     5581 gcaaagtcca gaaagtactc tgccagcagt atcttattct cagctgagag ttcttttgtc
     5641 gtttcctccg taaaactaat tccaagctgc tcctgtgcaa tctggatcac ctggtcgact
     5701 ttgtcgtaag cttctgctat ccgtcctacc agtttcgcct tgctgtactt cattttattg
     5761 tggagcattt cacggttttc tctgcattct ctgcggagtt cctggatatc tttttctacc
     5821 tgttccggac agtccatctc gccctgctca atgcggctgt gcatctgtcc gatcgcctta
     5881 tccagctcat atagctttac agaaagtata tcatgtgtca tacgcacacc cctttccttt
     5941 ctttctcaca ccattttagc aaaaatctgc ttcgctgata tagacaaaat atccgcagtg
     6001 taaaagaact ttacactgcg gatattttat aaatcgttat tctcatctga aacaaggact
     6061 gctttgccca ttaacagaag ataaatctga tgtactatat aactgatgtt ttcggtgtcc
     6121 tgcttcgtcc actcgcggag cagtcccata accgcctgac agtggtaatg caggataaac
     6181 tcccggtctt tgtctgaata accctcgtac cagtgctgca tctccgtcac tttctcaaac
     6241 agatcataaa aaacctgctc cagcatacgt ccgatctcgt caccatagtt ggactgcata
     6301 ccgcggatcg cataaggctg aagctggatc gcaatctcaa agaaatggcg aagccccttc
     6361 tcaagatcct gctgggcaat acattcttcc aggacaactt ctgtctcctg atacaaaacc
     6421 cagtggatca gatccggaat atcctcaaaa tgatagtaaa atgcctgcct tgtgatgtgg
     6481 cactcttcta caatatcctt taccgtcagt ttctttactt ttttctcaaa cagaaggttg
     6541 ctggcagctt ctgcaattgt ttttttcata tcaatagcca ttttacctac tcctatttca
     6601 ctcaaaaaac cgctttgttg cttatgctac cgtacatttt ttcttctgtc aagtaaatta
     6661 attctaaact gttcctgagg ctaatgatgt gcaaatgaac aaattcccca agaagacact
     6721 tttacgtgcc cttcggggaa ttcgttcatt tgcacattca gaaaatctcc gcatcttcct
     6781 aataaaactg atgtgctccg atcttcactc cggttccgga tcctgtatta aagtacagtg
     6841 ctcctcctac cggattctct ccgttcagtg ctgcctgagc tgcttctacg cagtcactcg
     6901 gtacaccgct tgccagtgca cttgccagtg ctccgctgta cgccggtgtg aactgtcctg
     6961 cctggtaaac aacctcggaa atgctgttcg ggaaggaagc acttgccaca cggttgagaa
     7021 caacggcacc gactgccact ttaccttcgc ggctctggtt gcctgcctcg cagtagatga
     7081 tcgctgccag aaggttcaga tcactggaat ctgctgtggt aacagtctct tcgcttccgg
     7141 ttgacgctgc agaatcgtaa gtgtcgtctg cggtatcata ggaagcatct gctgtcgtat
     7201 cttcgctgta gctgttgtcg gtataagaac cgtctgtgcc gtatgtacta tcggactcat
     7261 aagtttcacc ggcataagta ccgtctgtat aagaatcgtc tgtataagaa tcgcctgtat
     7321 tataagcatc accggtataa gtactatctg cattgtagct gttgtctgca taaatgctgt
     7381 ccgcagaagt atcatcataa gcaccatctg cactgtagct gttgtcggta taagtatcat
     7441 ctgtactata gctgtcatcg ccatagctgc tgtctgcata tgtatcctca gtatagctgt
     7501 catcgttata agtgctgtct gtatctgccg tgccatagcc tgctgcattc cagccccagc
     7561 cgttcggatc gtaatagccc tgtgccacgc tgtccgttgt gtctgtattg gcatctgcat
     7621 cactgacgtt gacatccgca gttgagattg caacggcacg gtctgtgatg atcgtctctt
     7681 cggcatcctc tgccggaacg tatgcaaccg taacgtcatc catctgtacc tgataccagt
     7741 caccatcctc ggaaatcagg ctgtactcgc ttccgttgtc tgcactgtcc agaatgtctg
     7801 ctgctgcatc cggtgaagag aacaggttta caccatccca gttgaccttg acgccctgtg
     7861 taccatagac atttgccaga tattctgcat cttctccgta tgccagataa tcattcttga
     7921 tatatccttc cacgccgttt gaaatgatgt gtgaccattt ctcgcccttt tcgatcaggt
     7981 ctgcagatgc tccctttgga agatagccga cgatctcact tttggtggat gcatttgcac
     8041 ggatgttgac ctgtgtatct acatctgcca ctgcacgata agaccacacg ctgtcttc
//
LOCUS       DAWDWH010000082         8048 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_4281_length_8048_cov_16.291880, whole genome shotgun sequence.
ACCESSION   DAWDWH010000082 DAWDWH010000000
VERSION     DAWDWH010000082.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8048)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4281_length_8048_cov_16.291880"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    910..919
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1747 a         1933 c         1473 g         2885 t
ORIGIN      
        1 cttccggagc cagtgaaaca attttcataa actgtttctc acgaagctct ctggctactg
       61 gtccaaaaat acgggttcct cttggagttt tgtcatcttt aataataact gcagcgttct
      121 catcgaatct gatgtaggaa ccatccttac ggcgtgtttc tctaactgta cgaacaacta
      181 ctgcttttac aacatcacct tttttaacaa caccgcctgg tgttgcatct ttaaccgtag
      241 ctacgattac atcaccaatg cttgcatatc ttctggtaga tccgcccata actctgatac
      301 acagaatctc ttttgcacca gtgttatcag ccaccttcag tctactttcc tgctggatca
      361 tgctgatata cctcctatat actatttcac tttttcgacg atttcaacaa gtctccatct
      421 cttgtccttg gacagcggtc ttgtttccat aacctttact gtatcaccaa tgctgcactc
      481 gttgttttca tcatgtgctt ttaatttata tgtgtctttc acgatctttt tgtaaagcgg
      541 atgttttaca tgctcttcga tagcaactac gattgtttta tccatcttat tgcttacgac
      601 cttgccgaca cgagtttttc taagatttct ttccatcttt aaccattgct cctttctagc
      661 agaatgttac cgttgattat tcagctttct ctgtaataac ggactggatt cttgcaatgt
      721 ttctgcgaac ttctttgatt ctgcttgtat tgtccaactg atttgttgcg ttctggaatc
      781 tcaggttgaa gagttccttt ttagcagcta ctaattcttc attcaattct gcagctgatt
      841 tcgttcttaa atcttctaca tacttattaa ttttcactgt tatcaccgcc ttctaaatct
      901 gccttggcan nnnnnnnnna catgacacgg cccggtttaa ctactgctac ccagtattcc
      961 agagttcctt taccggaacc catacgggtt tcagctggtt ttgcagttac aggtttgtct
     1021 gggaaaattt tgatccaaac tttaccacca cgtttgatgt aacgagtcat agcaacacgg
     1081 gctgcctcga tctggttgga tctgatccag catggatcta aagcgacaag accatattca
     1141 ccatttgtaa ttttatttcc tctaagcgct ttaccagcca tggagccacg gaactgttta
     1201 cgacgcttta ctctttttgg cattaacatt atttatcgct cccttcctta ttgcctttcg
     1261 ttggaagaac ttcgcctttg tagatccaaa ctttaacgcc aagtttgccg taagttgtat
     1321 ctgcttcagc aaatccatag tcaatatctg ctctcaatgt ctgcagtgga atagtacctt
     1381 cgctgtagaa ctctgtacga gccatatcag ctccgccaag acggccggat acggaagtct
     1441 tgataccctt tgctcctgct ttcatagaac gtcccattgt agatttcata gcacgtctga
     1501 aggaaacacg gttttcaagc tgcagagcga tgttctcagc taccagctgt gcgtctctgt
     1561 ccggtctctt aacttctttg atatcgataa tcagcttctt agaagtgagt ttctgagctt
     1621 ctgcttttac tttttcgatt tcagagccgc ctttaccgat aacaacaccc ggtttagcag
     1681 tgtaaatgat aacttttact ctgtcggaag ctctctcgat ttcgattttt gcaactcctg
     1741 cgctgtacag ttttttcttg aggtatgttc tgatcttatg atcttctacc agataatcag
     1801 cgaagtcagc ttcagcatac catttagaat tccagtcttt aataacaccg actctcaggc
     1861 cgtgtggatt aactttctgt cccatgcttt acctccctat ttctcattaa gcacgatggt
     1921 gatgtggctc attctctttt cgattctgta agctctgccc tgtgctcttg gtctgattct
     1981 cttcattgta ggtcctttat ttgcgtaaca ttcctcaaca taaaggtttt caacactcat
     2041 accgttgttg ttttcagcgt ttgcaattgc tgattttaat aattttgcaa tcagctcaga
     2101 tgcatatctt ggattgtaag ttacaatacc aagtgctgtc tgaacatctt tgcctctaat
     2161 agcatccaat acgaagcatg ctttctgaac agagactctg gcgtaagata acttagctga
     2221 cggtcttgtg tccttctgag catttctttc tctctttatc tgggatctat gtcctttagc
     2281 catagatata acctcctttt cgactgatta tccgattcta gcgaacgcca gattttttct
     2341 cgtccttacc atgtcctctg taggttctgg ttgctacgaa ctctccgagt ttgtgtccaa
     2401 ccatatcctc tgtaacatat acaggaacat gttttcttcc gtcatgaaca gcaattgtat
     2461 gtcctacgaa ctgtggaaag attgtagaac ggcgtgacca ggttttgata acctgtttgc
     2521 tgcctgctgc gttcatggca tctacctttt tcagtaagct tgcatcagca aatggtcctt
     2581 ttttcagtga acgagccatg gattcaacct cctatatcct atttattatt cagcgctttt
     2641 ccgtctcttc ttcttacgat cagcttgtta gactgtttgt ttttctttct tgtcttcaag
     2701 ccaagtgcag gtttgcccca aggtgtgcat ggacctggac gaccgatacc ggtcttacct
     2761 tctccacctc cgtgtggatg gtcattcggg ttcattacgg aaccacgaac agtaggtctg
     2821 atacccatgt gacgttttct tccggcttta ccgatgttga tcaggttgtg ttctccgttt
     2881 ccaactacac cgatggttgc acggcagttg atcggaacca ttctcatttc acctgaaggc
     2941 agacgcagtg ttgcgtattt tccttcttta gccataacct gtgcgccgtt tcctgcggaa
     3001 cgaaccagct gtccgccttt tccaggatac agttcgatgt tgtgtaccat agtaccaacc
     3061 gggatagcag acagcggcag gcagttacct ggtcttactt ctgcttcagc accactcata
     3121 actttcatgc cgactttcag tccggccggt gccaggatgt atgctttttc accgtctgcg
     3181 tagcagatca gtgcgatatt agctgttctg ttcggatcgt attcgatacc gataactttt
     3241 gctgcgattc cgtctttctt tctcttgaaa tctaccagtc tgtattttct tctatttccg
     3301 cctccgcggt gtcttacagt gatcttaccc tgattgttac gtccggagtt tttgctcaga
     3361 gatactacca agcttttctc aggagccgtt tttgtaattt cagagaaatc agatccagtc
     3421 atctgtcttc tggaaggtgt atatgggtta tatgttttaa ttcccattgt tgtttctcct
     3481 ttcaactatt tattgtcacg cttagcctgc gtatatcgtt tgattaagtc agatcttaga
     3541 gtccctggaa gatttcgata tctttgctgt cctctgtcag agcaacgatt gcctttttgc
     3601 tcttagctgt ttttccgact gtcataccac gtcttctctt cttgccgtcg atgttcatgg
     3661 tatttacgct ttttactttt gttccttcga acattttttc tacagcttct ttaatctgag
     3721 tttttgttgc ttccggatga acaaaaaagg tgtatttttt gtcgcccata gcgttcatgc
     3781 ttttttctgt gataaccggc tttaagatca catcatagta ctgtacattt gccattatgc
     3841 gtacacctcc tcgattgttt ctacagctgc ttttgtaact acaacggtgt tgtgtttcag
     3901 cagatcaaat acgttgatgg tatttgtcag agctgtctca acaccttcga tatttcttgc
     3961 agacagaact acgttctggt cgttgtcatt caggataacc agtgctttgt cagctttcag
     4021 gtttgccaga acatttttca tagcttttgt cttgatctcg tcaaatttca gttcatcaac
     4081 aacgatgaat ttgttttcct gaactttaga agtcagaaca gatttcagag ctgctctttt
     4141 ttctttttta ttcagtctga ttgtgtaatc acgtggcacc ggagcgaata ccattccgcc
     4201 gcctgtccac tgcggagctc ttgttgaacc ctgtcttgca tgaccggttc ctttctgtct
     4261 ccaaggtttt cttccgccac cggatacttc agaacgggtt tttgctttct gtgtaccctg
     4321 acgtttgttt gcaagctgac ttacaactgc cagatgcaca aggtgctcat ttactttcgc
     4381 accgaaaatg gcatcgttga gctccatagt gccaacttca ttgccttcca tattaaaaac
     4441 agatacgttt gccatctgtg tgttcctcct ttcgaaaatg cttacttacc agattttaca
     4501 gtctctttaa ttgtcactaa agatttttta ggtcctggta ctgcaccttt aaccaagaga
     4561 agattgttct caacgtcaac ttttacgatt tccaggttct ggatggtgat tctcttattt
     4621 cccatctgtc caggcattcc ttttcctttg aatacacggc tcggatcgga gcaggcaccg
     4681 tttgaaccct gatgacgatg gaatttagaa ccatgagcca tcggtcctct gtgctgacca
     4741 tgtctcttga tcgcaccctg gaatccctta cctttggaaa ttgcagtagc atcaatctta
     4801 tcaccagctg caaagatgtc agctttgatc tcgtctgcta ctttgtaatc ctcagcattc
     4861 tcaaatttga actctcttac gaatctcttg caggatacac cagctttgct gaactgacct
     4921 ttcattggtt tgtttacaag actttctctc ttgtcgccaa aaccaacctg tactgcactg
     4981 taaccgtcgt tttctactgt tttgatctgt gttactacac aaggacctgc ctgcagaacg
     5041 gttaccggag taagcactcc gtcttcattg aagatttgag tcattccgac ttttgttgct
     5101 aaaatcgctt tcttcattga tttacctcct gtttttctac agcggattac actgtgttgt
     5161 gcaatcatcc tagtcaaaca atcgatataa gtggaacttg cgttacttct atatcaaccc
     5221 tttaggataa tttacggaac aaattcatta tttgtttttc attttgatat cgatgtaaac
     5281 accagccggc atttccagtc tggagagtgc atcaactgtt ttctgagtcg gtgctacgat
     5341 atcaatcagt cttttatgag ttctctgctc aaactgttct ctggaatctt tgtatttgtg
     5401 tacggctctc agaattgtca ctacttcttt ctttgttggc agtggcaccg gtccgctcac
     5461 cctagatccg ttctttttta cagtttcgat gatttttttt gcagatgcat caaccagctg
     5521 atggtcatat gcttttaatg tgattctcat tacttgactt gttgccataa taaaaaagtc
     5581 gcctcctttt cgcactttgt ttcagtacga caagcggtga ctgtacacta tgccgtgtgt
     5641 gtctcaaatc tcacacggtg tttccggctt acccggacgt tctctatatt gtacagtgac
     5701 ttgtcgccag ttttataact tgacattcgc tccacggaaa acctgcgatt tgagtccgca
     5761 gcaacctcac gcttcaacgc tatcaatgtc acaacactgg ctattataat aaaaagccct
     5821 tgtaaatgca agagcttttt tactgttttt gttgtttttt tttaagtaca atccgtgttt
     5881 ccactcataa tctttacatc atctgctgaa gcagtgcctt gctttgttca aatgtataga
     5941 tattctgata aatgtcctcc gcctgatcct gatcaaaaag ttctttcagg ttctgctcat
     6001 actgttcttc tgacacatcc tcaccattcc agtaataacg gtaaatcagt tcaccgtttt
     6061 catccgtttg ctgatcttct gtttccccat aactgccttt ggcaactgta gtgaactttc
     6121 cgttttcaat cttataaatc tcgtcataat atccacccat atgtccaccg gttgtatgca
     6181 gcagattgct gccagggatt gtataggccg ctccatgtga caggtaaacc ttgtccgtcg
     6241 tgccgccatc cgttgtaaat acttctccgc ctgcataacc atatccataa tccagccaga
     6301 gctccggctg cccgtcgtcg ttgatatcca gaaaccgtac ttctgtcatg tctgttggtg
     6361 catcctgcga aaagatttca tttaaatagg aagaagtcca tccattttct gcccgcgtcg
     6421 gaagaatcgg tgcagccccg tagattcttt ctgtctttga cggatccaca tgaaacttct
     6481 cctgctcaat actttcttct ttgggataaa tgataagcgg tgttgaattt ccatccgcat
     6541 cataatcata atccagcaca aattcccccg tctcatcatt gtagttatct gcaattgtca
     6601 gggaacccgg catcgttgtt ttgatattac catccgcatc atacagatac agaaacgtat
     6661 attcattgcc cgtataggaa tcctgttctc cgtttccatt gtcataataa tcaacggaat
     6721 gaagcgtagt gctgcataat aaaccgttat cataataagc ataactgtcg tagtagatca
     6781 gtttgccatc cgcatcataa taattgattc gtgaaatctg ctgatccgtc tgctgcggct
     6841 gtatttcaga ttgctgcaac tgagattctg tttctgctgc tgtctcagcc gaagattcgg
     6901 attctgatgc tttactttct gtgctttttg tgttttctgt actttctgta ctatctggtg
     6961 cttttgtttc ttcagaaatt gtggttggtt cctgcggatc ggatgatgtt atgcccgttt
     7021 cctgtgcgga ggaagcacca cagccagaaa gaagcagcac tcctgctgtt acagctaaca
     7081 tctgatataa ctttctgttt ttcataagca tttccatttc ctttacacct atttcacttt
     7141 tacagtgcaa actgctttct tcttgccgga aatcaccgta atctttactt ttcctttttt
     7201 ctttgctttg atctttcctg ttttgctaac tgtcgcaatt ttcttattgg aagtcttgta
     7261 agtaatctta tcctggctgt ctgccggagt taacgtaact ttcaatctga aacttttgcc
     7321 ttttttcaaa gtcagtttcc tgcggttcag tttgattttt cgcgtggttt tctggcttgt
     7381 ctgcggattc tgatccggat ttgtctctga agaatctgtc ggcgaaaaat ttgtctttgg
     7441 tatcccctca taaactattt ttccgcatac actgcaagtt ttgctcttca gtccttttct
     7501 tttatttgtc ggctctttga caactttata tgctccataa ctgtgtcctg ttgcatttac
     7561 cttctttgta tctgtatttc cgcagatcgt gcaggtaaat gttttttcgc ctcgctctgt
     7621 acaggtcggt tctgttgtga tcactcctcc atcccagtta tggttctttg tttttgcaat
     7681 cgtcttaccg gaggaaattt tcactccgca atccgcacaa taagtatctc cactatatcc
     7741 tgcttccttg caggtaggat tcttctgatt gcggatttcc atatgctgat ggtccgttgt
     7801 gctcactttt tccgtttttg tattgccgca gatactgcag gtaaatgttt tttcaccttt
     7861 ctctttacag gttggttctt ttgtaatctc tccctgattc cagctatgat cctctgtttt
     7921 tgcaatcgcc tgaccggata agatcttctt tccacaatct ttgcaccagg tatctccgct
     7981 gtagccttct tctttacagg tagcttcttt tttattgcgg atttccgtat gctgatgacc
     8041 tgttgcac
//
LOCUS       DAWDWH010000083         8044 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_4282_length_8044_cov_14.076355, whole genome shotgun sequence.
ACCESSION   DAWDWH010000083 DAWDWH010000000
VERSION     DAWDWH010000083.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 8044)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8044)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8044
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4282_length_8044_cov_14.076355"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2358 a         1744 c         2065 g         1877 t
ORIGIN      
        1 acgccgttat gtacatcatc gcacagaaac tggtgtggaa gtcgaacgag gacggctatc
       61 tggtaggatc acgaggttcg gtcggatctt cgttcgtagc gacgatggcg gggatcaccg
      121 aggtaaatcc gctgcgtccg cattattatt gtgagaaatg ccactacagc gattttgatt
      181 ccgaggatgt gaaagcctat gcaggaagag ccggatgtga catgccggat aagacctgcc
      241 cggtctgcgg agcgaagctt cgaaaagaag gatttgatat tccatttgaa acattccttg
      301 gatttaaggg aaacaaagag ccggatatcg acctgaattt ctccggtgat tatcagggaa
      361 atgctcaccg ctataccgag gttattttcg gaaaagggca gacttaccgt gccggaacaa
      421 tcggtacgct tgccgagaag acggcatttg gttacgtgaa aaattattac gaagaacgcg
      481 gcgagcggaa gcgtacctgt gagatcaacc gtatcgtaaa aggatgtacc ggcgtgcgaa
      541 ggacaacggg gcagcatccg ggcggtatta tcgttctgcc tcttggcgag gagattgatt
      601 cgttcacacc ggtgcagcac ccggcaaacg atatgacaac ggatattata acgacgcatt
      661 ttgactacca ttccatcgac cacaacctct tgaagctcga tattctcgga cacgatgatc
      721 cgacgatgat ccgtatgctg gaggatttaa cagacatcaa tgcacaggac attccgctgg
      781 acaataaaga agtcatgtcg ctttttgtca gcacggaggc acttggcgtt acaccggagc
      841 agcttggagg atgtccgctc ggttcgcttg gtattccgga gtttggaacc gatttcgtta
      901 tccagatgct tgtggatacc aaaccgcaga gcttttcaga tctggtccgc atttccggtc
      961 tgtcccatgg aacagacgta tggcttggaa atgcacagac cctgatcgaa gaaggcaagg
     1021 caaccatttc cacggcaatc tgtacacgag atgacatcat gacttatctg atcggaaaag
     1081 ggctggacag cgagcagtcc ttcacgatca tggagagcgt gcgaaagggg aaaggactga
     1141 aaccggagtg ggaagaggag atgctggcac acgatgtgcc ggactggtat atctggtcgt
     1201 gcaaaaagat caaatacatg ttcccgaaag cacatgcggc agcttatgtt atgatggcat
     1261 tccgcattgc ctggtacaag attttttatc cgctggcata ttatgcggca tatttcagta
     1321 tacgagcttc tgcattcagc tatgagctga tgtgtcaggg acaggaaaaa ctgcttcact
     1381 atatggacga atatcgcaaa aaaggcgatg cactctccaa aaaagagcag gattccatga
     1441 aagacatgaa gatcgtgcag gaaatgtatg ccagaggtta tgattttctg ccgctcgatc
     1501 tctatcaggc agatgcgaga cgttttaaga tcatcgacgg caagttgatg ccggcgttaa
     1561 gtacgatcga gggtcttgga gagaaggctg cggatgccgt tatggatgcg gcaaaagaag
     1621 gaaaattttt atcaaaagat gatttcagag accggacaaa agtcagcaag acagtcatcg
     1681 acctgatgga tgatctggga attctgggcg atctgccaga gagcaaccag ctgtcgctgt
     1741 ttgacctggt aggatagata aggagaaaaa aagaatgagc gaagcaatcg ttaccctgaa
     1801 aaagggcgaa ggaagaatgt tgaaatcagg aggactgtgg atctttgaca atgagatcgc
     1861 aagtatcctc ggaagctttg aggacggaga tatcgtggca gtacatgatt ttgacggcta
     1921 cggacttggc aaaggcttta tcaaccgcaa ttctaaaatc cgtgtgcgga tgatgacaag
     1981 aaaccgtcat caggagattg atgaggcatt tctgaagatg cgtgtgcagg aggcatggga
     2041 ttaccgcaaa aaagtcagcg ataccggaag ctgccgtgtg atcttcggcg aggcagactt
     2101 tctgccggga ctggtggtag ataaattttc cgatgtgctg gtcgtacagt ccctggcact
     2161 tggcattgac cgcctgaaag atcagatcgt ggagctgcta aaagaagtcc tggcagcaga
     2221 cggcatcaag atccgcggtg tctatgagcg aagcgatgca aaagtacgcc gtcaggaagg
     2281 catggagctg tacaagggat ttatcggaga ggcgtttgat accaacgtgg agatcgaaga
     2341 aaacggtgtc cgctacatgg tcgatgtcaa agacggacag aaaacaggat tcttcctcga
     2401 tcagaaatac aaccgaaaag cgatccagca tctctgcaaa gatgcaaaag tgctcgactg
     2461 ctttacacac accggatcct ttgccttaaa tgccggatat ggcggggcaa aagaagtgac
     2521 aggagttgat gcttctgaac ttgccgtaga gcaggcaata ctgaactcca agttaaacgg
     2581 catggaggac cgcgtgaaat ttatctgccg agatgtcttt gaacttctcc cggaactgga
     2641 agaaaaaggg gagaaattcg atgtggtcat cctcgatccg ccggctttca ccaagtccag
     2701 aaattccgta aaaaatgccg tgaaaggcta ccgtgagatc aacctgcgtg cgatgaaact
     2761 ggtgcgtgac ggaggtttcc tggcaacgtg ttcctgctca cactttatga cctatgaact
     2821 gttcacacag accatccatc aggcggcaag aaatgtccac aaacgcctgc gtcaggtcga
     2881 gtaccgcacc caggcaccgg atcacccgat tttgtgggcg gcagaagagt cctattatct
     2941 gaagttttac gtgttccagg tggtggatga gaagtgattt tgtaaaatag tcacgccata
     3001 atgattcgaa aacaatccaa atgggaaaaa tcctggcagc atgttaagtg aaatccgccg
     3061 aagacgatat tctcaaatta aagtaatgaa aacagactcc tgtatcaaga aagaatacag
     3121 gagtctgttt tttggcagag tggaaaaaag gattgacgtt gcagcctcgt ctgttctata
     3181 ctagaggaca gagaaatcag atgattaaat gcggttttca ggctgcattt caggaggaat
     3241 aaagatggaa aagatcattt tgaccggcga cagaccgacc ggcagacttc acgtaggaca
     3301 ttatgttgga tctctgaaac gcagagttga gctgcagaat tccggagagt acgacaagat
     3361 ttttatcatg atcgcagatg cacaggcact gacggacaat gcagacaatc cggagaagat
     3421 ccgtcagaat attattgagg tagcactgga ttatctttcc tgcggactgg atccggaaaa
     3481 atgtacgatt tttattcagt cacaggttcc ggagctggca gagctctcct ttttctatat
     3541 gaatctggtt actgtttcca gactgcagag aaacccaacc gttaagactg agattcagat
     3601 gcgtaatttt gagacaagca tcccggtagg atttttcacc tatccgatca gccaggcatc
     3661 cgatatcaca gctttcaagg caacgactgt tccggtagga gaagaccagc tgccgatgat
     3721 cgagcaggca agagagatcg tccgcaaatt caacagcgta tacggcgaga cactggtaga
     3781 gccgaccgca ctgatcccgg agaatgaagt atgctgccgc ctgccgggta cggacggtaa
     3841 ggcgaagatg agcaagtctc ttggcaactg catctatctg tccgatacag aagaagaagt
     3901 tcgccagaag atcatgagta tgtacaccga tccgaaccat atcaaagtgt ctgatccggg
     3961 tagcgtagaa ggaaacgcag tctttactta tctggatgca ttcagcagac cggaacattt
     4021 tgagcgttac ctgccggact ataaaaatct ggatgagctg aaagcacatt atcagagggg
     4081 cggacttgga gatgtaaaag taaaacgttt cctcaatgct gtgatgcagg aagagctgga
     4141 accgatccgt gcaaaaagaa aagagctgga acagcacatt ccggagatct atgagatact
     4201 gaaaaaagga agcgaagaag caagaaaagc agcagcagaa acgatggctg aagtgaagcg
     4261 tgcgatgcgt atcaattatt ttgaagatgc agcactgatc caggaacagt cagagcgttt
     4321 tgcagctaaa catgagaaat aaatatcaga aatcaggcag attctttttc ttttgacaca
     4381 ggaaatgtga ccgtaaaaga agtaccgaga tttttggcac taaccaccgt gatcttacca
     4441 cggtggtttt ttgctatagt ctgagcgatg gcaagaccaa gtccataacc gccgtccgtg
     4501 cgggctcgcg atttgtcggt gcggtaaaag cgttcaaaca gatgtggcag gtcttcttcc
     4561 gggatggcct ttccggtatt gtggacagtc agctttattt tttcgttttc agatgtcagg
     4621 gtgacggtaa cttttttatc tgaactgccc gatttttcaa gaacatctgc aatgccggca
     4681 tatttgcagg cattatccag caaaatggca accagctgtc ggatctgtgg agcatcaccg
     4741 gatatgaaaa tatcatcaga aatttcatct tctatcatga caccctgctc aaaggcaacc
     4801 ggctcaaaag ttaaaagaca accaaggatc aggtcactga acgaaaacgt gctgtagagc
     4861 attggcgtgg atttcgcatc ggaacgggcc agaaagagca aattttctac aagctttttc
     4921 atacggccgg cttcttcctg cgtgctttcc acccactgga gttcatcgcg gatcgtggca
     4981 tctttgtgtg agagcagaat ccgaaggttt gcgagaatta cagtcagcgg tgttttcagc
     5041 tcatgggaag catcagcgat aaactgattc tgctgttccc aggccttttc gaccggtttt
     5101 aatgcccagc gtgccagaaa aatgctgacc agaagaaaac agagcagaaa aagcagaagc
     5161 atcatgacgc actgaatgac aagctcgcca agagcacgtg tttcctggac cgtatcggca
     5221 aaggcgattt ttgtgccttc cggtgtattc tgcttgataa aacgcagctt cagatttgaa
     5281 agatacccct gatcttcgga aacagcaaat gcggcactta cggcagcttc ggaaagcgta
     5341 tccgttacgg tataggagcc gctggagttg gtgtccgtga tctcgttgtt ttcatcaaga
     5401 gtgaccagaa aaaccggggt cgagttaaat gccggattgc gtccgcctgg accgaaatca
     5461 agacttggag actgaggttt cgtgtcattt ttcggcggct cctttgagcg tgagctgtcc
     5521 agcatctgcc gcattgctat ttcactttca gcacgaaaag cgtgtacacg ggtgaaacag
     5581 aacatgccaa gcaccacaca gaggatcacg gcaaccaggg acatgttgat gatcacaaat
     5641 ttgcgacgca gttttttgat catgatgttt cctcactttc cagccggtag ccgactttgc
     5701 ggacggttcc gatcgtgact ttcgaatcca gatagtggaa tttcttgcgt aagaacgaaa
     5761 tataagcctc cacattgttg ctttcggcag cagactctgc accccagact ttgacgatca
     5821 gcgtttcttt tgaaatcagg atcttcggat tagacatcag gattttgagg atttcaaatt
     5881 ccttatatcc aagatgaatg gatttgtttt tgcaggaaag gtcgttgctg gagagattga
     5941 gcgtcagatc atcaaacgtc agttcttcca gaatcacctc accctgacgt cttgagaggg
     6001 cacggatcct tgcaagaagt tcttccggga caaatggttt tgtcatatag tcatccgcac
     6061 cgctgtcgag gcctgtgatc ttgtttttta catcgtcaag agccgtcagc atgatgaccg
     6121 gtgtggaaat tttggactcc cgcagatgat gtacgacctc aaaaccatcc atgccgggca
     6181 gcatcacatc cagcacgatc acatcgtaat cgccggaaga agcataggca agcccgtccc
     6241 ggccatcgta aacaatatct gcctgatatc tggcttcctg cataatctgc cccagtgcat
     6301 ccgccagtcg cttttcatcc tctataatta aaatctgcat aaaatcgcct cctgtgtata
     6361 taagatcaga cattgcagac agtgcctgcg aatgaatatt ataattgatg atgccatacg
     6421 aattgctctg tactgcgtac ttgcgaaatt ctgtaaccct ggcatcatca gtatagcata
     6481 aaaaactgaa aacaggctga aatatatcac agtcagatat ttgatcaaat caggcaagtt
     6541 ccctgctttc attgagagta tccgcttgac tctccgacta aaatatgttc gacttaaact
     6601 tcgtgacatg agcgaaaatt tcgtttacaa tagatttcag gtccgcacaa ggaacaaagc
     6661 aaacaagttc agatatcata cacagcgact gatcatcaga taaaatccaa aacaaaatag
     6721 aagaaaagag gaagaagcat atgaaatcat tagtgatcgc agaaaaacct tctgtgggaa
     6781 gagatattgc aagagtttta cattgcagca aaaacattcc gggagcactc gagggggaca
     6841 agtacattgt aacctgggca ctgggacatc tggtgacgct tgcagatccg gaagaatacg
     6901 ataaaaaata cagcaaatgg gacatggaaa cactgccgat gatgccggaa aaaatgcagc
     6961 ttgttgttat cagacagacg gcaaagcaat atgggctggt gaaaaagcag ctgttccgca
     7021 aggatgtaaa tcagattatc attgcgaccg atgccggaag agaaggagag ttggtagcac
     7081 gctggatcct ggaaaaatca gaatgcaaaa agccgatcaa acgtctgtgg atctcctcag
     7141 tcacagacaa agccatccgc gaaggatttg caaacctgcg tgacggcaga gcgtacaaca
     7201 atctttacca tgcggcaaaa gccagggcag aagcggactg gctggtcggc atcaacgcta
     7261 ccagagcgat gacctgcaaa tacaatgcac agttgtcctg cggccgtgtc cagacaccga
     7321 cgcttgcgat ggtggcacac cgcgagaaag agatccgcag ttttgtaccg aaggaatatt
     7381 atggcatgca gattttatcc ggcggcgtgc gtttcacctg gaaagatgca aaaagctccg
     7441 gttactgcac atttcagaaa cagagaatgg atgaaattga gaaaaaagta aaagcagaga
     7501 tcaaaagcgg caaaaaaatg atcatcaaag aggtgaaaac aacagcaaag aaaacatttg
     7561 caccggggct ttatgatctg acggagctgc aaagagatgc caacaaacgt tttggatatt
     7621 cggcaaaaga gaccttaaat atcatgcagc gtctctatga aagccataaa gtgctgacct
     7681 atccgcgaac cgattccaga tatatcacaa ccgatgtggc agatacctta aaagaacgtt
     7741 taaaggcatg cagcgtcgga ccttaccgta aactggcagg aagcctttcc atgcgtccaa
     7801 tccgcaaaaa tgcttctttt gtggacaata aaaaagtgac ggatcaccat gcgatcattc
     7861 cgaccgagca gtttgtggat ctgcagacga tgacgaacga agagcgtaag gtctatgatc
     7921 tggtagtacg tagattcctg gcagtactgc ttccggcatt tgagtatgat cagaccgtat
     7981 ttaccggcgg gctggcagga gagagtttta cggcaaaagg taagattgtg aagagccagg
     8041 gctg
//
LOCUS       DAWDWH010000084         7868 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_4379_length_7868_cov_16.200051, whole genome shotgun sequence.
ACCESSION   DAWDWH010000084 DAWDWH010000000
VERSION     DAWDWH010000084.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7868)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4379_length_7868_cov_16.200051"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1795 a         2146 c         1532 g         2395 t
ORIGIN      
        1 cgctcgcccg gttttagttc tccttttaaa atcgcctgcc gcaaagtctg gaacacaaca
       61 tcccgaagtg gaaggtattc attcatctcc agttgaaaat cacctgtcat ttattttcct
      121 ccgtatcttt ctgatttttc catatctttt atctggatac cctacgaatg gtttcctgtg
      181 cccacaatcc agccatcttt acatagctgt ggttacgaac atctgtcgga tcttgtcttc
      241 ttcacgcaga agttcggccg ccctttctgc ctgctcacgc tcgttaaaaa tgccgaatac
      301 tgtcggaccg ctgccgctca tcatggcgtt taccgcaccg cactgcatca ttttatcttt
      361 gattgtctgg atgatcggat aagccggtat ggtaaccgtt tccagcacat ttcccatttt
      421 tttacagact gcttccaggt ctccatcctg caaagcccgg atctgtccgt cgatatccgg
      481 atgacaggta agctcgttgg ctctcaggtt ggtgtaaaca aactttgtgg acacgttgat
      541 cggcggtttt gcgatcagaa caaaacattt cggcagagac ggaagcgggg ttaaaatctc
      601 accgattcct tccgcaagtg ccgttccacg cagcacacag aacggtacat ccgcaccgat
      661 cgccacagaa cgctccatca gctcccgttt ggtcagccca agctgaaaca tacggttcac
      721 accgaccaga accgctgccg catccgaact tccgccaccc attccggctg cagtcggaat
      781 gaattttcga aggtcgatct ccacaccgga agtgacctga aattcatcca tcagaagctt
      841 cgctgctttg taaaccagat tgtcttcatt gaccggcaga aaactgacat tggtcttcac
      901 acgaatctgc ggttcttcca aaggactgag cctgatctgg tcatacatgt taatgctctg
      961 catgatcatg cggacttcat ggtagccatc ttcccttttt cttgtgacat ccagtccaag
     1021 attgattttt gccattgctt tcagtttgat ttctttcatt tgcacctctg tatctctctg
     1081 aattactttt attttcatga tgccagattc aaaggaatgt tctgtttttc cataaaatgc
     1141 gtatctgaat ttgattctta gcatcatttt ttgcattttg tatacaattt cctctatatt
     1201 gtatcactaa aaatacaaaa tcgtcaatag gatgttttgc tgttcttttc tcaggaggcc
     1261 gtatgaagga ccgctgattt cacgatcagt agtttctgtc ctgcccggat ttcttctgtc
     1321 agatggttca tgtttctgat atgttcggat gtggtgtaat actttttcgc aatgtcccac
     1381 agggtatcct ccggctgtac cacatagcag acgatcccag ggcgttcctg cagttttttc
     1441 agatccagct ctttctcttc gatatccaga atacagttct ggtgcatgat ccgaacagca
     1501 aacagattca ggctcagggt tcctttgatc tccacttctt cccctgacat ggatgcggaa
     1561 agctgctcga tctgcggatg cagatggaaa cggcattcac tggtgatgcc ttctgcttcc
     1621 atcagatgtg aaaacgggat cataccggtc acgagtgcaa agggatgctc gtcgtccgct
     1681 gtgatataca gaacagaaat ctcgatggct ccctccattt tgatgccctg ttcagtcttt
     1741 gcgatttcat ccactttgac ctctccgcgg caatggcaga tctgcagcag acgtggactg
     1801 ttctctgaaa tacggattcg atctgaaatt ctgctctttg cataatttcg gatcagtagg
     1861 ctttcataag attcttcccc gaagaccggc gtcagctctt ttgacggtgc atagacatct
     1921 tctaaaatct gcacagtatc ttcttcataa atgcggatgt caagctccag aacggcatcg
     1981 agctgcagga ttctttcctc tccatcataa tccggccgca cttccagcgt ccgttctgca
     2041 agcgtcacct cgatatccgg caccatctcc ggccgacagc cggtacaatc aatctgtccc
     2101 tgaaacggaa gggcggattc gatccactgt ctgcttccat tttcatcatc cgcagcataa
     2161 agtacgaaca caaacagttc tccccggata tccagcacgc cgtctcccgg tcttacatcc
     2221 cacgaccgaa gctgcacgct gtcccataaa atctcaccga tgttcggttt gttggaagat
     2281 aagctgatct cttctttgat ccggaaaatg tcttttttct gtactgccat ggagagcggc
     2341 tcaatcttct ttgtacgcac actgacatcc ggcatctctt tcacttccac ggccgcctgc
     2401 gtatcataaa gctcctcaac ggatgcttca aacgtcacga gtgcctggat actgatcttt
     2461 ctggaattga tcagactgat ccgaatatcc tcggttttcc atttcagact gacattatct
     2521 cccggctttg ccccttccag gttcagcttt tccgaaaacg gcagttttgc ctgaagacgg
     2581 taaacgggat gctgttctcc ttccgccagg cagagcatcc acacttccag atttccgctt
     2641 accagaatct gattttccat gatcttgctc tcttctaccc ggacttttgc acggtgctgg
     2701 atgattcctg tgacatctgc tttactgtcc gggacattca gatcttcttc cagcgtgatc
     2761 tgattcattg cctgacttct ctgacggctc atccgaattt tctttttgac gatttccata
     2821 aaaaactcct ctcgcatcat ctcaaatcaa tgtatgcgaa aggagttccc cttattacgc
     2881 tttatggttc aaacgtgacc tcctgatcca tgttctcgat caacaggatg atgcatggat
     2941 acgatgcctc cgctgccgcc tgctctttct gtggcgggat cagtgtggtc gtcacatgca
     3001 gcccgtcgcc cataagctcc acggcatcgg cacggatgct gtatccgccg gtcggctgct
     3061 cgccgtatcc ccggatgatg tatctgcctt catcgccctc ccaggtcatt ttgatctctt
     3121 cttttttatg ctcttcgatg atctcctgta acttctgcgg tgctttgtca aaagcaacca
     3181 cctgaaatgc aacggaaatc cgctcttcct gtgctccacc cgagaggcat ccgctcatcc
     3241 cggcaaaaag aagtatccag cataacaata cgatgacctt ttttgctgtt ctgatcattt
     3301 tttgcccttt tcgcttcctt ggaacagtat acggtctgtc ttttttattt atgcctgaac
     3361 gaaatatttc cgctttgatc tgcccggaat attatttgca tgcctcaact tcgatggatt
     3421 caccgaaaat ctcgatcgcc tttacctgac tgatgtcgat cggcacgcga aagtcaaaat
     3481 tgagttctct ggaaactttc acagcatctg ctgcttcgtc atcaccggaa agttcacagt
     3541 ccgcaatccc catttcatag tattcttcgg ataacagact gccgtttttc aggcggatat
     3601 ggacgttgtc aaaatacttg ccggaatctt tctgcgactg cggcagtgtc atataaatcg
     3661 aacatcccat cggagagagc actatgcgat taatcttgac gtcatcttct ccatcagaga
     3721 cggtctgatc acagaccttc ttataggaaa tatcctttgc ctgatccata tccacagtaa
     3781 acttccaggt tccggaacag atggaaaccg gattctccaa tatcccggaa tcttccgaag
     3841 cggaataagc agatacttca tcatcggtac tttctgcctc gtcttctgtc ccatcctcct
     3901 gatagtaacc gaaatcctcg aaggtaaatt catactgatc gctgtcaaac ccggcttctg
     3961 ccgaataatt tgtcacataa acaaactggt ttgaggcaag actcttgtcg tcctcctgat
     4021 aataataagt gccacttgcc atcagatcct gctctgcatt cacatcggta ttcagaaagc
     4081 tataagcatg ttcctcctgt ggtccgcctg ctgtcacaat ctgatcctgc tgctttaacg
     4141 tcccctccac gatcatggaa aaacggttgc cgctcttgat cagccctttc agatccagac
     4201 ggatattatc gtcctcgtag actgtacctg cggttgtcac agccatgtct gtcagctgtt
     4261 ccagatccat caccgcactg tctgcgacat ggtattcttt tgcatgtttt ttaaagaatg
     4321 ctttgaaatt ctccgctccc ataagtgcat aagcagccgc tccgaccgta ccgaaggtaa
     4381 acagcagaaa gcttgccgca actgccccat agcgtacaaa gcgatattgc caccggcgtt
     4441 tctgtttttt aaccagcccg tccattttcc ggtaaaaatc atcggacagc tgaatatcct
     4501 gtaactcttc ttctgaaggc agagcatcca tctcctcttt atggtagctg caaactgctt
     4561 cttttaataa tttctcaaat ttcctgtcgc tctctttcat attgcagcac ctccttcatt
     4621 ttctttttgg cacgatacag gcgaatctcc acgttctttt tgctgatatc cagaatcttt
     4681 gcaatctcgc cgttgctgta ttcatgaaaa tagcgaagct ccagcaccag acgaagcttc
     4741 tccggcagaa tcttcattgc ctctaaaagc tccttttctc cctcttcctg tatcacattt
     4801 tccagcggct gatccttctc gccatggagt gcataatcca ctttctccaa atcggactgg
     4861 agaccgcttt tggatacacg cagcatatcc aaacatttgt tttttacaat gatcacacac
     4921 aagcttgtca tctcctgcga tgtcagatgc cgataagtgt catatttctc tgcgagtttt
     4981 acaaatgcac cgtgaacggc atcctccgct tcttctttgt ttttcagaat tttcagagct
     5041 acataataca tggtgcggta attcttctca taaatctgcc caaactccag ttttgtgttt
     5101 tcgtcaccaa gcccgcttat cagtagtaac attttttctc cccttgcagc catgctgctt
     5161 cttattattc aagagtgcct ctggcattct tactgcgagg tgcacgtcat gcatcgcatg
     5221 agatattctt ctgtgcacct ctgcaatccg ccggaggcta tatcagatgg gagatggatt
     5281 cccgttactg atacaagctt gctacccgcc gccgacagaa acgggagtca tctttcatct
     5341 gatataacga tccctgttct tattttgcta caaaatttgt tctataaagt gtaaaacttt
     5401 cgctgtgtca aacttttgtt ctccaaaact tgctgccggc atcatgccaa gcagtgaatt
     5461 tgtcacaaag catccgttac acttactgat atcctccgga tacagttctt tttcttccag
     5521 aagtactccc cttcttccgg cttcttttat aacatatctc cgcatcactc ccggcagcat
     5581 cccgctgctg acgggcggtg tgaccagttt atcatcctga ataaaaaaga tgtttgtcgt
     5641 acagccctcg ctgatctgcc ctttctgatt gagaaaaatc ttttcatcca gcccgctttt
     5701 ttttgcattt cgcttttcca taatgttgtc tgcaatccca agcgtcttat ggtatgtgag
     5761 cggtgaagtc tcatttcgtc tgatttcgga aaatccaagc cgaaatcctt tctgatactg
     5821 ttcttctgag taagtgtttt ttctcagtcc aaacaactta ttctgtgccg tcactgtgat
     5881 cttgagtgct ccgtgctgct tttcttccga ctgtggacag gcattcagat aagcaagcac
     5941 ttccttctcc tctacgctct ggtcaattcc gaaaaatgca aggctgtcac gcagccgctc
     6001 cagatgccag gcaagaagct gcggtatgcc ttcttccaca gcgatcgtct caaaggctcc
     6061 gatgccaaag taaaacccgt catccgccag aaccgttgat ccgctgcttt tcttttcttc
     6121 tgttttattc tgcatttcct gcccctttca gatacgctcc agaaaagctt cgaggtcttc
     6181 gaccgtatcc acgatccatt ttgcaccgca cgtttccagt tcttccctgc tgccgtatcc
     6241 atagagtaca ccgacacagg aaatcccgaa tttgccggag ccgaggacat cgtgtttgcg
     6301 atccccgatc atcagcactt cctgcatatc cgtgatctga ttgtctgaaa gtgccgactg
     6361 tattacatct tctttgtgga ttttgcttcc atccatggaa cttccctgaa tatcatcaaa
     6421 atactctgcc agttcaaaat gcttaagtat ctgttttgcg taaatctgcg gcttggaggt
     6481 cgccaggatc acttttttgc cctgttctct gcattttttc agcaaatgcg tgattcccgg
     6541 ataaacttta ttttcgaaaa tacctttcac ggcataatat tcacggtaaa gacgcacgcc
     6601 ttcctctgct tttttttcat caaacccata aaattcttta aaagaatcta caagaggagg
     6661 acccacgaaa cggtaaagat tgctcttttc ttcggaaatc cccagatttc caagtgcata
     6721 ggaaacggaa ttcgtgatcc cgtctgcgga gtccgtgatc gttccgtcca ggtcaaacaa
     6781 cagatatttt atcactttct tttctccctt ccaacgcctg cagaacagct tttgctttct
     6841 gaagcgtctc ttcgtattca aactctgtct ccgattcgca ggtgatgccg cctccgactc
     6901 cgagatagta ctttccgttt ttatgtactg ctgtgcggat cacaatattg aaatcgcagt
     6961 caccgtcgag cgtcagataa ccgatcgagc cggtataaag attgcgtcgg ctgtgctcca
     7021 gttcttcaat gatctccatc gcacgcagtt tcggtgcccc ggtgatggat ccgcccggaa
     7081 aggctgcttc cagaagatcc acaacatttt tatcttccgc aagtcttccc tgaacttccg
     7141 ataccagatg aaagaccgtc gcatacgtct cgatgctgta catttccgtc actttgacgc
     7201 tgcccggcat acagacacgg ttcagatcat ttcgctccag atcaacgatc ataagaagtt
     7261 cgctcttatc cttatcggac tcttccagtt cttttctcat cgccgcatct tcttctctgg
     7321 tggctccgcg ttttctggta cctttgatcg gtctggtcgc cacaacaccg tcacgcatct
     7381 gcaaaaaacg ctccggagac gcactgacga tctgcagatc cccataattt aaatagccgc
     7441 caaacggaga aggattgtct ctgcgaagtg agcagaaaac atcgtaaggt gtacgagtac
     7501 tttccacagt aagatgctgc gtcatatttg caatatagat atcaccctcg acgatatagt
     7561 cgatcatatc ctgcaccgcc tgcttatagt cttcttttgt gaaatttggc gtgactctga
     7621 tctcactgtg atcatccttt gttcccggaa tctgaccgtt tttccacaga gtatacgttt
     7681 ctgcgactgt gttttccaca tcgtcaagca gcgtatccac ctctttggtc tgaccgtttg
     7741 ccacaagata aaaacgtttt tcctgatgat cctcaatgat aaaattatca taaaaacaga
     7801 ggatcacatc tggcatatcc acctcttttg gatgtctggc cgctacgttt tctttctctc
     7861 ttccgtaa
//
LOCUS       DAWDWH010000085         7846 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_4388_length_7846_cov_10.892568, whole genome shotgun sequence.
ACCESSION   DAWDWH010000085 DAWDWH010000000
VERSION     DAWDWH010000085.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7846)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7846)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7846
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4388_length_7846_cov_10.892568"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1813 a         1881 c         1342 g         2810 t
ORIGIN      
        1 ctcgttccac tttcaattcc ggtcgcgtaa agcatgcata atctccttcc actatgattt
       61 taaatccata tcccattgtt tttcacctct tctttcataa aaaataatct tgtgcttcca
      121 ataaaattcc taaattttcg tcataataat cttcatttgt cagacagtat attcccgtac
      181 caaaatcgtc tgcaacatgt tgttttatca actcatccaa ttctttctta gtaactgtac
      241 aggtatactt ttgcagctcc ttctcatttt cgatttttgt atatctcaga gtatccacca
      301 gttttctgct cttttcatct cttggtacaa caatagaaca tgtcctatca gggatcagtt
      361 caaattgttc tgcataagtc tgaaatggca gattttcaaa agacctgcac tttatatgca
      421 tcgtattttt ctgaatattc tcttgtttta agaaaaacac ttcttcatag tattcttcaa
      481 tacattccgg ctgtgaaata tctttatact ccgccaacaa acgttgcgta atattcatct
      541 tgtcattctg tgcttcattt tcaaattcaa acacaaaaac ttcaccctta tcccgctttc
      601 cttctctgtt acatctgcct cccgactgga gaatactgtc cagaccattc aattcacgaa
      661 aaacggtata tacatccaaa tcaactcctg cctcaataag cgatgtagaa ataatcgtaa
      721 tccttctctc ttccggtata tttccatctg gaaaatcttc atccaggctt ttcagttctt
      781 ctctgatagc ctgcagaact ttttgtctgt catatgcagt catataagtc gataagtgat
      841 attttcttcc tgtacacatc tgaaaaagtt gtcttgcatt tttcttcttg tttacgataa
      901 tcaatctgga cggatacttt tcacttttag ctaatagtcc ttccggactt acttttccca
      961 tattgatata ttgacatttt tgaaactttt caaaaagcgt cttatccttg atcaaatttt
     1021 cgattcgatt atttgccggt gcataaagct tcagcaactt ttcaaaatca ggcatcgttg
     1081 ccgtaagaaa aacagcttcg ctgttcagat atttcgttat ataggcaatt ccctgaagac
     1141 atggcagtaa ataatcctgc ggcatcatat gtgcctcgtc aaaaatcaga atactgtctg
     1201 ccatattgtg caattttcga agttttcctc ttttatttga ataaatgctt tcaaaaaact
     1261 gtactgctgt tgttatgata aatggagcat cccaattttc tactgctatt ttagctgctt
     1321 ctcgataatc ctcatttaaa tccaccttct catcataaga aaaagttgac tgatgtctta
     1381 aaatctccag attatttcca aaaagttttt caaaaacctc tgctgtctga tcaataatac
     1441 tgttatacgg gatcacatag ataattcttt ttttcttttt cgctattgct ctttgcagtg
     1501 ctattttcgc actcgctaat gtttttccac tgccggttgg catatttata aggtagatgt
     1561 gtgcatcttt tgtactgttt cgatatgcct gctcctgcag caagcttctc gtcttctgca
     1621 attctgtttt tacttcaaac gcctgtaact gaacatttat tttttcaaga cacttttcaa
     1681 aatcagcctg caattttctc actacttctg tacttctgca aaactccgcg gtgtctgttg
     1741 aatctgcatc taccagacag gaaaataaat atcttgtcag aaatgtaaac ttatcaatta
     1801 accaatccgc atttcttccg caatccgcag caagaaactg ctgccatttt cgaatatcta
     1861 attccggcag ttccagttct tgcttgtagg catcgtaatc ctcaaacgtc ctgttcattc
     1921 tgcctttcag actgttatcc tcatcatttc caaatccccc atttgggatt cccgtgtgat
     1981 gtcctgcaat gcaatactgc atcatcagat tcattggctt tggatacttt tcacccactg
     2041 caattgctcc acaggttgaa tgttctacac gaatattttc ccctcggatt cttctttgaa
     2101 atccctcctg atactttccg atatcatgtg cttttcctgc cgcataagct agatcttcca
     2161 tttccggaat tgcatattct ttgcaaagat ttgccgtatt ctcacaatgt tcccggacag
     2221 tttgtacctc atttgtatac tcatttctgt gtgcaatata tatttccccc atattgctgt
     2281 accctcctgt attctcataa ttcacttata ccattctttt tcttttatgt caattttttc
     2341 tttatttagt taaatataat ttacatatca ttatatcttt tatcagctga tcaaaatttt
     2401 caaacacaac tgtgtaaaca tattaccttc tgactcggta ccataataaa aatgaccgta
     2461 tgaaaacttt tcattttcgc atacgatcat ctttatactt aacaaaataa tttctactca
     2521 catgcacaac ctgtacagac tttccaatta tattttatct ttcctatttt ttcaactgct
     2581 tagtcaaaat ctatcttaaa tcttacccgt caaaaaagac cagcactttc gcactggtct
     2641 ttttccttac ttcaacttta tttcttgttt cttactttgt gatcttcacg cttgccttct
     2701 ggccttttcc tttcagcagt cgtttgtagc gtgtcagttt cttcttcggt actttgatct
     2761 tacatttttt gtcaatacct ttgaaggcct tgcttccgac tttcttcagc tgtgtggatt
     2821 tgatcgtgat ctttttcagt ttctttgcac cgttaaatgc atttgcaccg atcttgttta
     2881 cgtttttgcc aatgataacc tgcgtcagtt ttttgttgtt cttgaatgca ttctttgaga
     2941 tctcggttac tttgaaggta acgccgtcaa tttttacagt tccaggaacg gtaaatttct
     3001 tttctgttgc ttttctcggt ttcaccagag caacggttcc gttctttgca tcggattttg
     3061 ttactttgta gaatcctctt ccaaccggtt tggttgttcc gacagcaggt actttctgag
     3121 tattcggatt tgtgtttggc ttttctgttc caggctgtgt gcttggtgtt tccggctgtt
     3181 tattcgtttc ggtttctttg ccagtatccg gctcgctgcc tgccgctttc agattctgct
     3241 ctgctttttt caggctgtcc agtgctttgc ggacgtctgc ggcacttgca gtcttagaag
     3301 cggctgctgc ttttgctgct gccagtgcat tctggaatgc attccagctt gcatcactgt
     3361 aatcgccttt tgttttgtct tttacttctg caatcttgct gttcagtact gcttttgctt
     3421 cttctaactg tacatttgtg gtcggatctt cctcccattt tgcaaacagt gtcagatctg
     3481 cacttaccgg tgatgctgct gtatatgcta ctgtgcagtt tacatcgctg taccagccct
     3541 tgaatacata tccggctctg gtcggattct gcggcaggct ccagctttcg ccggctgcta
     3601 ctttcagaga ctcaatgctg tttccgcctt ttgtatcaaa agcaacggtg atttttttct
     3661 gtgttgttgt gttcttaatt gcttttccgt aaacttctac ttcgcagata tggtttccgc
     3721 catgtgctgt tccgcttgtc agaatacttc cgcgtgtgta aatacggata taacgtactt
     3781 tctgttcttt tgcttcaaat gtctttcctt ctgatgtttc tgcatatttt gcatccggat
     3841 ttgcaagctt ttcattgctc agcagtccgt atgcatactt ggaatctgaa acgctgctgt
     3901 acagcacggt atcatttgca tcaaaagcag catcttcaga tgcaactacg atcgtatcct
     3961 ggtaggtacg tccgtccgca aaataacgcc acatttttac ctgatccagc ttgaatacat
     4021 cgccaagatc gatctggaag tatacgctgg ttcctgccag tacatcactt tcaatgctgt
     4081 ccagtgtaca ggtaaaggag tttgaacctt tatcaccatc gatcattttt tccggactac
     4141 tttttttgtt tgaaacatcc tgatcattat ctgcacgttt tactgtgatg ttggaagcct
     4201 gcagagggat ttttccgttt ccatccggaa caataccgga atctccgctg ctgtctccgc
     4261 caactgctga tcctgctttt tccagtgcag ccagcttaag tgttgcatct tttacttctg
     4321 cctggcttac ctgaagatca gttccgcctc caacagtcag agttccatct ttcaaatctt
     4381 tgatgttttt ctcatatctg cttgcatcca gtgtaccgtt gacataaact ttcagactgc
     4441 cattcttttc acggatacct gcaatgtgtt tccatgttcc atcgtttaca gcttctttgg
     4501 aagttacgga tacatctcca actgtaaaga ctgcttttcc gtctttgtca atggaaaggc
     4561 tgtaagcacc ttcctgtttc agaatgttca tattgtttcc ggttgttttt acataagaag
     4621 atacggcaaa atcagcggtt cctgtaacag ctgttccgtt cagctcatat gcttttgcaa
     4681 ggccaagtat tgtctttcct gccagtgctt cgtccgttgt ttccgttaca tcatttgcat
     4741 tcttcagatc tgtttttgct gatgctttcg ctacaacctg attttcctga tatacaactt
     4801 ctgtatttac tgctgctgca ttgccggtta aatcttttaa tccacttaca gaaaccgtta
     4861 ctttgcttcc tgcactcaga gtggatgtag tcagcattac ttttctctga tttgcactta
     4921 cttctgcttt tgtaacattt gctccggaaa cggttgcagc aggagtaccg gttacgcgct
     4981 catcaaatgt cagctcaatc gtgttgtccg cagttgcttt tgcaagtgtc agctgaggtg
     5041 cttttgtatc ttttgcagta gaaagagacc agatacgtgc ctcacccggc tgcagtgtaa
     5101 ctgtgatcac atcgccgtat tttactgtct gataatcggt ctgtgcatcg gttgtcttgt
     5161 gatccagaat tgttgtacgg ttgagtgtct tgcctttcag actttctgcc gcaccaacgt
     5221 ttcgatccag tgtaaagctc agtgtcttta cggatgcact tggatttcgc atggaaataa
     5281 taccttcttc accatcccag caggagtatc cgtaggtatt gcccttatcc ggtgtcgtac
     5341 cgatcagttt cgcatttttc aaaatgtgat aatttttctt agcccactcc agatattctg
     5401 cattgatatc ccatttctga ccttcatcta tcatttccgg gctgtagtac agctcccaga
     5461 atgccgttcc tctggttccc atcatataca gatacgtttt gaactcatca tctgtcatct
     5521 ggtttgccag attggttcct gtttttccat agattggatc atggttgtaa atgtttgcca
     5581 gtggaaactg gaactctctt tcttttacaa aatcaaaata tctgccgtca cggtaagaaa
     5641 gcagctgatc tacctgtctg ttctgtccga cctgaacacg tccgatatcc tggctgttct
     5701 ggatccagat agaatttgcc cactgcagat accatggact tgggtttaca taacaggtca
     5761 ggttaaacca cagattgtca atgccgtttt tctctgcatt ggtacgcagc gtctcaaaca
     5821 gatcgatcca tgcttcccag gtatcggaaa aataatacat accattcttt ccgcctgtca
     5881 tatgtttatg atttgcattt gtacatggct ttgtcgcaaa tccatccagt ttccagtaat
     5941 ttactttata agcatcctgc atctgtaaga aaaactcagt cagtttcgtc acataccgtc
     6001 tgtcaccgac atcaatatca tcggttaccg cattgtatgc accaaaacct gctttttcaa
     6061 taatcttacc cattgcagcg ttatagttat aaccacctct aggtcctacc catacaccaa
     6121 atccggagcc gaagctttct gcaagatcgg acgcaggacg gaatccgttc gggaacttat
     6181 tgttaaactc ccagaaccct gtcacattat ccgtggtacc gcagcgtgcc acatcatgaa
     6241 cagtagaagt tgtattcgag ttgttataat tattccatcc gtcatccaca acatacgaat
     6301 ccatcggttc tacgccggat tcagaaaatc ccttttccat ttttttgaat gcagattcaa
     6361 tgttgtcatc gttaatggtc atcatattat cgtaccagga attgtactgc gtacggaaat
     6421 cgcttcttgt tgcaatgctg ttgatatatt cataaaagtc agactgaatg acattcatat
     6481 ccgtacttct tgcagcaccc gctacggtct gccaggtcac atattttccg tctgtggtaa
     6541 gctggttatc ttcctgcagt ttttcgaatg tcttgccgga aaaataacgc atatgtgcat
     6601 ttttattgct gtcgatctct gtttctgtca tcgggaactc acatccaagg aacattccct
     6661 gaatataaac cggctggcca agagaaatga catatccact cataccgcca acgccttcac
     6721 ccatgatcgg atgtgtccac acggtatcat ttgcattgac attcaacgat tccagatcga
     6781 tataatcaat tgctgctttt tctttgtcac ttacttcgat ttccaggaat ttacgcatga
     6841 aatgatctcc gtcatccatg gtaactttca tggtaacatc ccagtctaca ccgtttttgg
     6901 aaacagtctc aaacgggaat tctaccatga ctccgccgtt cgtatctact tttttaactt
     6961 tgctgccttc cagagtcatc tcgctggatc tgatcacggc acttgcatct gctgtaccga
     7021 ctgctttgct gtacatcttt acttctgccg ccgttgccca tgcctgaccg tccgtctttg
     7081 gtgatacagc aaccagtttt acataacgac cctttttcgg tgctgtcaga ttgatataaa
     7141 tcgtttctct gtcattgtaa gtaaaatcac cttctgccgc aaggtttgca tcactttcca
     7201 gatctgtttc actgtttccc acatacagtt tatatccttt gatatcgccg tttgtggaac
     7261 tgtcctgacg tggctgataa gaaaatgcgg caatttcctg ttcgctcttc atatcaaaag
     7321 tcagggaatg tggatatggc tgatccggtg aatacttgct gtgccagatt gtagatgcat
     7381 caccatcaat aacattggct cccggaccgt cttttgcacc tacttcatta ttgtactgat
     7441 cgcttgcagt aactgtccag tttgtgcgat ccagctctcc gtccagtttt gttgtaccat
     7501 cctggcgaag tttgataatg aattcttcgg aaccctgtgc cggtgtaaac gtcagatctg
     7561 ccctcttatt ttccagttct gtcgttgcca gcttgttgtc tgaagtagaa aatttacgct
     7621 ccagatattc atttccgatc gtaacttctc cgtttttcac ggagacttgt gcttctcctg
     7681 atgccgcctg gacggtcgtt ccaatccctg ttgctggaat accggtcact accatggccg
     7741 cactcaaagc aattgacaac cattgttttt ctttcttttt caaaattttc ctccttgttt
     7801 gtgcaatatg cataatttta ttcgtctccc cccgcttttt atagta
//
LOCUS       DAWDWH010000086         7783 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_4422_length_7783_cov_10.451216, whole genome shotgun sequence.
ACCESSION   DAWDWH010000086 DAWDWH010000000
VERSION     DAWDWH010000086.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7783)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7783)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7783
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4422_length_7783_cov_10.451216"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    868..877
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2197..2206
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3810..3819
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5188..5197
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6898..6907
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1799 a         2019 c         1536 g         2379 t
ORIGIN      
        1 tgcagacaac gtttgaccgc ggctcgttac ctcagataca ctcgaagcat ccacaggatg
       61 tcattggttt ttcaatgata gaatacaggg atacgtcttc cagagcaccc tgggagaatt
      121 tctgaactgc ttcgtttacg tcttcgtatt caccgatacg ttcatcgatc ggtctttctt
      181 ttgtgataac ctggcatggt ccctgaggat ccagctcgtt ggttgctttt gcatccagcc
      241 atgaaacagc tccgcaaagt cccagtcttt ccggtgtaac cacgcatacg tgggatgggg
      301 agaatgcctg gcacatgata cagctgtaat atacatcaac gccttcgtct gtcagagtat
      361 tcagacgttc atcacgtttg tcaaacatgg ctgtagctac ttcgtgacgg attcttgtac
      421 attctgcagg atctgtgtag atggttacct gacatttatc tacaacggct gcaaattcgc
      481 tcttaacttt tgcgtacaga acttcaccga tatgttttgc acggaatcct gcttcaaatg
      541 tctctttgct tatacggata cggatcatat cacgctgacc ggtatgcatc agaccttcga
      601 tacagttcag atatgaatgg aatttacgct caaataccgg ctcaaaatcg gactgcatgc
      661 tctttccggc cacctctact acataacaga tgctctgttt acttccaacc ggcatttcgt
      721 cgatatccgg tcctaccagt gtgatcttgt gatcttcgat ctcggatgct tcttttgtca
      781 gaaccagctc gcagcagtct acacgggaac catcgaactc aacctgcata tcgccacggc
      841 ggatgatctc gccttcaaat gcagatgnnn nnnnnnngtg atcttgattt tgatgtctct
      901 tgcttccaga gaagttgcgt taaatttgga aacatccggc tgaacgatca ggctcttcgg
      961 aactttgaaa gtatcttcgt ttgtgattac cgggaaacca agtgcgatcg caccggctcc
     1021 acatgcaacg ataaccggat ccagcggtgc aaatgcattt acgaatgccg gtacacgttc
     1081 catggtgtat ttcatcagac ctgttgcatc gcccggctga atgttaccga agatcagagc
     1141 agctcttact gcaacggata caacatgtat tacacttgta acgtctttgc ccagagggat
     1201 cacacgtacg tttgcacctg ttttgtaacc cagttcttca cactgatcaa tgatgcctcc
     1261 aaccagtgta accaggatac cctgtgcctg atagctcttt accaggtcaa caccctcctg
     1321 tgcagtcggt gcagcaccaa ggataactgc aacacccggg atatcacctg ttaccagcgg
     1381 tacaccaagt tcacggatca ctgcatctgc cagatgtccg tagcacggag cttcgtatgg
     1441 tgcagctccg tcaatatatt tcagtacttc gataaattct gcacacagag cggttgcaat
     1501 accggacata aatgcatcat ttaatttctg ctctcttgtc atcatggttt ttacaacgcc
     1561 cagagcttct tttaactgtc ccagtgtagc aacctttgta ccggttactg cataataaca
     1621 tggcaggctg taagccgtat ccgggaaagc aactgctttg tctgcaccat gtttttcgat
     1681 cgccgcatca atggcacctt ctgtcaggcc atatacggta tcatttccgc tgaatacaac
     1741 atcaaataat gtcactttat ttacctcctt tggacagatc tgcgtctatc ctgcttttta
     1801 ttattgaaat ctctgctctc gcgttcgggc ttacatcaaa cggtgatttc ccctgacggt
     1861 ctgcatcgat tatttctgaa ttgtaatgaa taaatccaag caaatcttcc tgtggtattt
     1921 tggaacggat aaatgcttca tcatcctcgt tccttacttt gttggcaacc acgcgtacct
     1981 ggcggactcc aagttccgca gcaagtctct ttacattttt ataagtctgg atacttcttg
     2041 cccccggttc gatcacaaca ataaactgat ccatgccttc tgttgtccca cggccaagat
     2101 gctcaagccc ggcctccata tcaagaatta caacatcgtt gctccttaac accagatgat
     2161 tgatgatgcg cttcaacatc acatgttccg gacacannnn nnnnnngccc tcctgtgcat
     2221 acagtctggc aagtgtggct gcaaaggtcg tttttccgac accgcctttt ccggtaactg
     2281 caattttcat atcactatcc gccttctgtt tttctttgca gggcggcaga agccgccctg
     2341 ccatttatta tctgctcgga aatcagattc ccagtgcagt tctcttctct tcgattcttt
     2401 caatcatagc atcgcccagt ttgtcgatgt ctttttctac ggtataagca gcttctaccc
     2461 agtcacgtac cccttctgtc agcagctgtg cgatcttgtc agagccggat acatacggat
     2521 caacgcccag gaacgtatcg ataccggtag ctactacata gttaccgatg gatactgctt
     2581 tttcggacat ccattccgga gcacatccga caaccggaag ctgagaaata ttcagtccgg
     2641 aatcttttgc cagctctgcc gccaggacca tcatacgtga aatatctacg caggatccca
     2701 tatgcagtac cggtgggata tcagccagtt cgcatactct cttcagacct gctccgcaga
     2761 gttcttttgc ttctttgtcc atcagacctg ctcttgcggc agcctgggcg gaacatccgg
     2821 atgcaataat gatgatatcg tttgcgatca ttttcttcat cagctcgata tgggcggtat
     2881 ctggtcttac ttttgggttg ttacatccaa ccatggcaac tgcaccacgg ataacgccgg
     2941 atgtgataca ctccagaagc ggttttgttg ttcccatctc atcaacctga gagtttgcaa
     3001 cgccatccag tgtttttaca attgcttcta cggaatatcc aaccgttgct ttctgtttca
     3061 tatccgggat atgtaccaat tccggttttc tgttcttgaa gttttcgatt gccatcttaa
     3121 cgatcgcttt tgcgttttct cctgctgtct ctgcattgaa acggatgtat tccgcatccg
     3181 gcatctgtgc gatcggagaa gtggtcacaa acttagtatg gaaacattta ctcagagggc
     3241 caagtgccgg gaagatacac tgaacgtcta caacgattgc ttcacatgca ccggtcaata
     3301 ctacgttttc ctgctgcagg aagttacctg ccatcggaat accacgacgc attgcaactt
     3361 cgtttgaagt acagcacaca ccggatacgg tgatcccctt tgctcccatt tcttttgcgt
     3421 aagcgatcat ttccggatca tctgcatatt cacagatcat ctcagacagt gaaggatcat
     3481 gtccatgaac aacaatattt acgttttcag caaccataac accaaggttt gcttcggttt
     3541 cgatcggttt cggtgttccg aacagaacgt ctgagaactc cgtacctgcc atggaacctc
     3601 cccagccatc agacagaccg gctcttaagg actgacggat cagtgcctca ggaagggaag
     3661 aacatcccat gtggctcata tgtaaagaac atgatacttc gcggtcgatt gctcttggtg
     3721 cgatttcttc tctctcccag atctcctgtg tatgctgagg agctctcttt aagaagttct
     3781 gtgtgccgaa tactttgccg tattcgctcn nnnnnnnnna atccaagata tgccatctca
     3841 tgtgccagat cgtaaatatc ttttccttct gtctcaacac cccattcttt tgcgatacgg
     3901 agcagcttct caggatcttt taccttgtaa ggtccttctg gttttgcaca gtacagtgtg
     3961 tggcagatct cacggccgtg atcagaatgc gttgccgctc cgcctgctgt aaatttcagg
     4021 aagttacgtc ctacgatacc gtgtacatca catccgcaga tacctctctg agccttcggt
     4081 gtgatacggc atggtcccat tgcacagata cggcagcata caccctgctc accgaattta
     4141 caatgtggtg tctgttttgc tgcacggtac tgccatgcgt ctgctcctac tttttttcct
     4201 gtctccagaa gtttctctgt agccgcttcg aattcttcaa cggttgtcaa tttaaaattt
     4261 cccatcatct acctccttgt ttatgggttc ccatcccaca ggtttatatc ctatccgccc
     4321 ccatgggata caggtctttg aaataaatcc cctcacggct tcccgaaagg ggatgtgctt
     4381 tataaactga tagagtccag tatcttatac aatgcagtct ttactgccgc atcatccgga
     4441 agctctgtca ttggttttcc gtcacagtca tattcataga cttcctgatc ctgtggaaca
     4501 acgccaaaga gggtaagtcc ctgcttttca atctcttctt ttgttccttc attcagtttg
     4561 ccgtccggtg cacggtttac gatcagcccg atctgtttcg gattaagtcc gcactctttc
     4621 accagtccgg cgatgcgtcc aaccgcctga acgcctcgtc ttgaacagtc gctcaccaga
     4681 atgaccgtat caaccttcgg cagaataccg cggctgatat gttccattcc cgcttcgtta
     4741 tctacgacca tatatttata attcggtgtc agtttctgta cctgagcctg aagcagacca
     4801 tttacaaaac aataacatcc tttgccctgt gtcctgccca tgacgagcat atcatagtca
     4861 tcttcttcga ccaggcagcg attcatcatg atctctgcat agtctgcttt tgacatactt
     4921 ggcgggatcg ggctttttgc actgttcgct gcattttcaa tctgctcacg aacttcaccg
     4981 agtgtcatct ccacttcaac gccaagtact tcgtttaagt tggaattggc atccgcatct
     5041 actgcaagaa ccggtgtttt ccccttctca caaagatact gtatcaaaag cccgcacagc
     5101 gttgtctttc ccacgccgcc ttttcctgca acggctatta cctgtgccat aaatatttcc
     5161 tcctgttgcc tgtctcttta taccagtnnn nnnnnnnatt gatgatttca gaaacatctt
     5221 tccatttgtt cagagcatac agatggatgc cctcaacgcc cagtgcacgg tatgtatcga
     5281 tctgtctaat cgtgtattcg ataccttctt ctttgaattc tgcgattttc ttatcataga
     5341 ataaatcaaa aggtttgccc tctgcatctt ttgctgcaaa tggatttggg aacagccagt
     5401 gtctggagat cagtctggaa agttcacgat ccattacaca gccgttacgt gacagtgcca
     5461 tattgatcgt agctgcctga tccagaattg gcataactcc tacgtctacc ggcatggtaa
     5521 cacctgcttt tcggattgca tccagccagt atttgaactg ttccatatcc cagcagagct
     5581 gtgtcatgat gtagtctgca ccattgtcct gtttctgttt cagaacagcg atatctgctt
     5641 ccaggcttct gcaggaaatg tggccttccg gtgaacctgc tactgcgatt tcgaatttat
     5701 cgccgtactg gtcacggaca aatttcacca ggtcggtagc gtaatcgaaa tctcccatgg
     5761 tgcttgtctc acccatcggg aagtcgccgc gcagtgccag catatggtca accccttctt
     5821 ccaggtactg atccagctgt tctctgatac cttcttttgt atttttgatt acggtgaaat
     5881 gtgttacagg gatggaacct gctttcttga tcatctgaca tacttcacgg tttgctccaa
     5941 cgtttccgcc gccagctcca taagtacagg aaatgtattc cggtttgaac tccatcagat
     6001 gttcgatcgt tcctggcaga tttgccatac ctttctctgt ttttggtggg aataattcaa
     6061 aagagagaag ctgtttctct ttcatctttt ccgataattt catagtaaca tacctccatt
     6121 ttattttaat ttatttgata actgctaaga ttccgtgcag ttgatcttca caatgctgtt
     6181 tgcaaggtct accattacga tagtaccttc cagaactttc tgctcgccca ttacatccgt
     6241 aagggtgatt gtctctccgt caacatcgat cctgcttaca aattcaaaca gttttgtctc
     6301 cggatcctga gctttgtata ctgctgcaag acacataatt ccgcctcctg ttaattttct
     6361 tttacagtgg caagtgctac actcagatac aggttgcctt tctggaattc atcaaccgct
     6421 ttccgtatct gttccgccgc tgtatccatt ccggctttcc gcaatttgtc tgccatctga
     6481 tccagttctg ctgcatgatg ttcattgtgc tgcagcatat atgttaaaag tgcaatgttt
     6541 tcttttttcg gatcatgtga ctcacagccg ctgcagcctt cctggcatac atcgtcgtgt
     6601 gtatgtgcat gtccgtgctc atgaccatga gggcaaacat tgccattctc atcttttatg
     6661 atatgcactg atatctactc ctttctgtac ctgcaaaatt acgcaaatac ccttatattg
     6721 acaacattat aacacagcgt ttatgaattc tcaacaagag atgatgtatt ttttttaata
     6781 atatcaggga tatcgacgaa agcatatcct cttctaaaat ttacatcata ttctcaaaaa
     6841 tctgaaataa tatagctata attgccacga tcaggcaaac aatgatacgc accctgcnnn
     6901 nnnnnnntcc ggcattattt tttgccgttt ttttcttttt atgcggcttt ttatcactgt
     6961 cgtatttttt atgcaattcc atgtggattc cttccatctg cggaagcaca tagttttccg
     7021 tctgaaaccg tttatcatct tcaaaaagat ctacttcttc cattccgggc ggtctgttga
     7081 aataagcaca ctgcggacag atcccatcgt atttatcata tttgaaaaaa gttttacaac
     7141 gtttgcactg catctgcatc cctccgtttc cactcaggca aaaagaaaaa gcctgttgat
     7201 tccatgataa gacattatcg tgaaatcagc aagctttttg cgtttttctt acgcaagcat
     7261 cattctgtca tttgcaaatt ctccgccgct tgcctgttca aacttggcaa gtagatcgct
     7321 gacctgaagg tttttctttt cttctccggc cacatcataa ataatccggc cttcatgcat
     7381 catgatcaga cggtttccaa gacggatcgc atctttcatg ttatgtgtta ccatcatggc
     7441 tgtcagatga tgctccgcaa tgatcttttc ggtaagtgcc agtacttttg cagccgtttt
     7501 cggatccaat gctgcggtat gctcatccag cagaagcagc tgcggtttct gaagagaagc
     7561 catcagaagt gtcagtgcct gacgctgacc gccggaaagc agaccgactt tgcttgtcat
     7621 acggtcttcc agcccaagat ccagggcttt taactgttct ttataaaata cctgctcatc
     7681 ttttccgatt gcccatttca atccacgctt tttaccgcgg cggtatgcaa gtgccagatt
     7741 ttcctggatc tccatgccgg ctgcagtacc tttcatcggg tcc
//
LOCUS       DAWDWH010000087         7719 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_4462_length_7719_cov_10.023878, whole genome shotgun sequence.
ACCESSION   DAWDWH010000087 DAWDWH010000000
VERSION     DAWDWH010000087.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7719)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7719
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4462_length_7719_cov_10.023878"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    845..854
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1603..1612
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5360..5369
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6496..6505
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7480..7489
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2253 a         1504 c         2151 g         1761 t
ORIGIN      
        1 tgcactgctg accatctacg atatgtgcaa agcactggat cgggccatgg agatcacgga
       61 tattcatctg ctggaaaagg ctggcggaaa gagcggacat tataagagat catgccgtta
      121 aatgttgaga ttgggagcaa acggacgaaa atagagtgtc caatatgcta aaataagggg
      181 gaatccatat gtatcaagtg ggaatcgtca cattgagtga caaaggagca aaaggtgaac
      241 ggcaggattt gagcggtccg aagattcagg aattactgcc aaaagataag tatgaagtaa
      301 tttcatatac gatgcttccg gatgaccggg aaaagatccg cagggagctg ctccgcctct
      361 ctgatgaggt gaaatgtgat ctgattctga caacaggcgg aaccggattt tcaccgcgtg
      421 atattacgcc ggaagctacg atggatgtgg cagagcgtaa tgcacctggg atcgcagaag
      481 gactgcgtgc ctgcagcatg cagtttacaa aaagagcaat gctcggaaga ggtgtttctg
      541 tgatacgaaa aggaacgctg atcatcaatc ttccgggaag tgtcaaggca gtaacggaaa
      601 gtatggagtt tctgatcgga aatattgagc atggactgga tattctgatg cagacggaca
      661 gtgaatgtgg aagagcctga aagacagggc agaaggagtg aaagaaatga aacggatgaa
      721 aacaacggag gcggtcggac aggtactttg ccatgacatt acgcagatca taccgggtgt
      781 gacaaaggat gccgttttcc gtaaggggca tatcattcgg gaagaagata taccggtgct
      841 gctcnnnnnn nnnngtgctg ctcagcgttg gaaaggactc tgtctatatc tgggaaaacg
      901 atgagacgat gatgcatgaa aatgatgcgg cagaagtgct ttatcgtatg acggcaaatg
      961 agcatatgca tccttcggat gtaaaagaag gcaagatcga agcgatcgct gatacggaag
     1021 gcctgctgaa agtagatcgt gagaaactga agaaagtaaa tgcattcggg gagatgatga
     1081 ttgcaaccag acacggcaat accgtagtga aaaagggaga taaacttgcc ggaacacgta
     1141 tcattccgct ggtgatcaaa aaggataaaa tgcaggaagc agaaaagatc tgtgacggaa
     1201 caccaatcct gaaaatcctg ccgtttaccg taaaaaaagc agcgattatc acaacgggaa
     1261 atgaagttta tcacggcagg atcaaagatg cctttacgcc ggtcattgag cagaaaatcg
     1321 cagaattcgg ggcagaaatg atgttccatg agatttttga tgacaatgac agaaagatca
     1381 cagacggctg tctgcgtgcc atagaagcag gggcggagat tgtattctgc accggaggca
     1441 tgagtgtgga tccggacgat aaaacgccgc ttgccatcaa aaataccggg gcggaaatga
     1501 tctcttacgg ctccccggtt ctgccgggag cgatgttttt attgtcctat taccggaaag
     1561 gtgacaaagt gataccgatc tgcggacttc cgggctgtgc cannnnnnnn nngtacaaca
     1621 ggcggaccat ttttgatctg gttctgccgc ggctgatggc agcagatccg gtcacggcag
     1681 atgagctggc ttcgcttggc gaaggtggac tttgcctgaa ctgcaaagtg tgtacatttc
     1741 cgaactgcgg ttttggaaag ggctggtaga agatgctgga tcagttcggg agaaagatag
     1801 aatatatgcg gatttctctc acaaacaact gcaatcttcg gtgcagctac tgtatgccgg
     1861 agaagcagat ttctgatgtg catttctttc cgatgaatca agttctaaga tgcgtggaaa
     1921 gtgccgtttc ttttggcatc acgcattttc ggctgaccgg tggagaaccc ttatgttatc
     1981 cgaaaatcga agaactgctc tgtaaaatca aacagatcaa aggagtagat tcggtgcatc
     2041 tgaccacaaa cggcgtgctt ttaaaagaaa aggcggcaca gctgaaacag gcaggcatcg
     2101 acagcatcaa tgtcagcctg gatacgccgg atgaaaaaga ataccgggtt ctgaccgggg
     2161 gcggaaagct ctcaaatgtg cttgatggga tcagaaaggc agcagaactt gaaattccgg
     2221 taaagatcaa tgctgtgctg cgagaacaga ccgatgtctg tgcactggca gcgtttgcag
     2281 aacaaaacca cgtcacactc cggtttatcg agatgatgcc gattggtttt ggaaagattc
     2341 tgccggtcga tccaaaaagc aaagtactgg aaactttgca ggagcgatat ggcaggtatg
     2401 agaggatcat gcaaagaaaa agaaaagcag attctcagga agaatatggc tatggtccgg
     2461 cggtatatta tcgattttcg gatctgaata tccacatcgg tctgattcag gcgatccatg
     2521 gaaaattctg cgaccgatgc aaccgcatcc gcatcacttc cgaggcaaaa ttaaagcctt
     2581 gtctggcttc tgcaaaagtg atcgatctgc gtccggcact ggaagataca gaaaatccag
     2641 acaagctggc agagctgatg cggcaggcga ttttccagaa gccgaaatcg catcattttg
     2701 aggagcagaa tccggaaaaa agttcagaaa ccatgaatca gattggtgga taagattaaa
     2761 ggagaaatag atgggaaaac tggtcgcgat caatatcagt gaaaaacgcg ggacagagaa
     2821 aaaagaaata caggaagcgg agctggttgc agatttcggg attgccggag atgcccatgc
     2881 aggtaaatgg cacaggcagg tcagtctttt atcttttgaa aaaatcgaag atttcaaagc
     2941 cagaggagcc agaatcgaaa acggagcctt cggggagaat ctgatcgtct ccggatttga
     3001 ctttaaaacg ctgccggtcg gaacgaggtt tcagattgga gatgcactgc ttgagatgac
     3061 gcagatcgga aaacagtgcc attctcactg tgccatttat cagcggatgg gcgagtgcat
     3121 catgccgaaa gaaggcgtgt ttgccgttgt cttaaaaggc ggcacgatca aaaaaggtga
     3181 tgaggtgacg atgatagcgg caaattttta tgcgacagtc cgggatcgga ataaggcagc
     3241 ggacacattg acagcgaccg tcattaccgg aaaaaacagg ggcgaaaagc tctgcatgat
     3301 ggatggaaaa atccgtgcgg tacgaagttc cggtgccgga atgtatcatg gtctgcacaa
     3361 acacgatatg aatgaagagg caaaagaatc aatatccgga tcagattttt ttcatgaaaa
     3421 acatgccgaa gaaatctgga gggcgcatct tgcaggcaaa caccggatca cgatcgaaga
     3481 gcaggagatt tttcttcatt ccatcggaaa cagggcaaga cttgtgatct gcggcggcgg
     3541 acacgtagca actgccctgg tgaggatggc aaaactgctg gattttgaaa tctgggttct
     3601 ggaagaccgc ccgttttttg cagaacatgc aaaacaggag ggagcagatc acattctttg
     3661 tggagattat gtggaaagtc ttgcaaaaat cccgaaagat gtggataatt attatgtctg
     3721 catgacgaga ggacatcgtt ttgatctgga gtgcttaaga gagatttata aaagtaattt
     3781 tgcttatgtg ggcatgatgg gttccagaaa gcgaagcgtg ctggtgcgaa aagatctgga
     3841 agcggcaggc tatacaaaag agcaggtaca aaagctgcat tccccgatcg gtctggcaat
     3901 cggagcacag actccggcag agattgcact ttccgtgatc agcgagatcg tgcagtgcaa
     3961 aaatgaacgt gcaaaagcgg cagaagcaga cgaggcaatc ctggaagagc tggcagagcc
     4021 gcagagactt tcaaaatttg cagtgaatga tgaaaatgag atggaatacc gcatgctttg
     4081 tacgatcatt gaaaactggg gcagttcacc cagaagcatc ggaacccaga tgctggtcac
     4141 atcggacaac cgtatgatcg gaaccatcgg cggaggctgt gcagaagctg aagtgatcac
     4201 cagatgtcgc ggatattttg cggaaatgcg aaaaggtata catgggatct gcgagattgt
     4261 caaaatccag atgagcaccg ataacgtcga agaagaaggc atggtgtgcg gcggcaggat
     4321 tgaagtgctg ctggaggaga catgacaggg catgcagcac tatggtatgc aggaactgat
     4381 cgtaaatgta cagaatcttt gtataaactt atagtggaaa tggattataa aaataaagag
     4441 catttaaaaa ttgcatcatg aaaggaaaaa gcaaatgaaa aaaacgatcg cagtaacttt
     4501 cgcaatcgta tcactggcaa ccgcaggagt tcttcttgca ggatgtggaa gcaaggaaaa
     4561 gaaaacagaa gttaccgttt ttgcagcaaa gagtctgaac ggtgttatgg aggtgctggg
     4621 cgaaaagtac aacagtgagc atccgaatgt aactttccag acaaactatg acagttccgg
     4681 aacgttgatg acacagatca aggaaggtgc aaaatgcaac attttctttt ctgccggtgt
     4741 ttctcagatg gatgagctgc aggcaggttt tgacggtggt gatatcgtgg aaggaagccg
     4801 gcgggatctt ctgaacaatc aggtatgcct tgttacatgg aaaggcagcg ataccaaagt
     4861 aacagatttt tccaatcttt ctcttgcaaa gaacatggca ctggcagatc agaccgttcc
     4921 ggttggacag tataccagaa aagcactgat caatgcggac atggcaggct cagagtacac
     4981 agaagtcgat cagctgacag atgatgtgat atcaaaggca ctgggcggcc tggaaatcaa
     5041 taactgtgca aacgtaggag ccgttgcatc cgcagttgca gaaggagcaa atgaagtcgg
     5101 aacggtttat tattctgata cgttcggata tgaagatcag ctggatatta tcgagcagct
     5161 gccgaattcc ctgaccggtg atgtgatcta tccggtagca gcagtagaaa gtgatcatac
     5221 ttccgatgaa gaactggaag cttccgagga tttccttact tacctgcaaa gtgaagaagc
     5281 agtgaaactg tttgaaaaat atcattttac ggtacagtag gagatatcta tgaatttgca
     5341 gaatctggat tttacaccgn nnnnnnnnng aaaaccggag tggctgcaac gtttgtttcc
     5401 tttttccttg ggatttttgc ggccagattt gtgatgggcc ggaagggaaa agcaagagcg
     5461 ttctgggatg gttttctgac gatgccgctg gtacttccgc cgactgtggc aggatacatt
     5521 ttgcttcgta cattcagtac aagaagaccg tttggccgtt ttctggcgaa cagtcttggc
     5581 attcaggcag tacatacctg gcttggctgt gtgctggcgg caacggtcat cgcttttccg
     5641 ctgatgtacc gcaatgcgag agcggccttt gaacagatcg atgagaatgt gatctatgcg
     5701 gcacagacgc ttggattgag cgagcggacg atcttctgga agatccgcat accgatggca
     5761 aaaccgggca ttttatccgg tacagtcctg gcatttgcaa gggcgatcgg tgagtatggg
     5821 gcgacttcca tgctcgccgg aaatatcgcc ggcaggactt cgacgatctc gcagcagatc
     5881 gccatggtgc tccagaacgg agattttgca cttgccggtt tctggtgtct ggtgattttt
     5941 gtgatttcgt ttgtgtgtct tgttgcactg aatctgtttg gagagaagaa aagtaaaggg
     6001 aaaaagcatg tctttcatcg taaaaataaa aaaacaactg gctctttatg agctgttggt
     6061 tgatctggaa acggaagccg ggattctggc ggtgatcgga ggctccggtt ctggaaaaag
     6121 catgacgctc aaatgcattg ccgggatcga gacgccggac agcggattta tctcgctgaa
     6181 tggcagggtt ctgtatgatt cttcaaaaaa gatcaatctt ccgccgcaga aacggaacgt
     6241 cggctatctt tttcaggagt acgccctgtt tccgactatg acggcagctg aaaatatcag
     6301 cattgtgatg aaacaaaaga atccggtcca ggtgcagaaa tggctggaag aatacggtct
     6361 tggagattat gcggacagtt atcctgatca tctttccggc ggacagaaac agcgacttgc
     6421 catgatccgc atgctggcgg ccgagccgca gtgcattctt ttagatgagc cgttttccgc
     6481 actggatgag catatnnnnn nnnnntatca agcggaaaat ggagcgggaa gtgatggaga
     6541 tgctgcatga ttttgacaaa ccgatccttt ttgtttcgca taatcaggag gaggtttatc
     6601 gcctggcaga ccggattggc agcatggaaa acggcagatt cagtccgatc cgtgaaaaga
     6661 gggcgttttt tgcagagcct cagacagtcg ggcaggcaaa actggtgggc tgcaataata
     6721 tttcagagat tgactggatc agcgaagacc gggtgtacgc aaaagactgg aatctttcgc
     6781 tgagagtccc gtttactgag ctggaagagc agaaaaaata tcgtgcgatc gccatttatc
     6841 cgggagatat cactctttat tcagaagatg ccatactgga agaaaaacaa gttaggaaca
     6901 aaatttcagt aaaatcctgt agcaccgaag aagaactgca cagctgggcg gtaagctgtc
     6961 agatagatgg aaccgatgcg gaacttatga tgcttttatc gaaagaaaaa atgccgtcgg
     7021 taccggaaaa aattacagaa atctttgtgg atggcggaaa gatccatctg ctgcagtaaa
     7081 gatgatgcaa tatgttttac agaaagtgtg aggatgacag catgatttat ctggacaacg
     7141 cagcgacaac gcttcaaaaa ccgcaggcag tttatgatgc agtttatgat gccatgaaaa
     7201 ctttgggaaa ttccgggcgg ggattttccg gtgcggcact tggagcatcg agaatgatct
     7261 atgaggcgag gcagacgctc tgcgattttt tcggaggcag tgatccatcg aggctggttt
     7321 ttacgtccaa tgcaacggaa gctttgaata tggcaatcca ggggatgttt cttccgggag
     7381 atcatgtgat cacgacgcag ctggagcata attcggtgct gcgtccgctc tatgctatgc
     7441 agaaaaaggg tgttgaactt acgattgcgg cgagtggacn nnnnnnnnnc atccgggaaa
     7501 acacgaaagc gatcgtctgc acccatgcat cgaatctgac cggaaacctg acggacatgc
     7561 cgaggatggg agagcttgca aggcggcatg ggctgtttct gatcgtcgat gcttcgcaga
     7621 ctgcaggtgt gattccgatt gatgtaaaac agatgcatat cgatgtgctc tgttttaccg
     7681 ggcacaaaag cctgtttgga ccgcagggaa ccggcggac
//
LOCUS       DAWDWH010000088         7653 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_4500_length_7653_cov_14.550145, whole genome shotgun sequence.
ACCESSION   DAWDWH010000088 DAWDWH010000000
VERSION     DAWDWH010000088.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7653)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7653)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7653
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4500_length_7653_cov_14.550145"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4342..4351
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4869..4878
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2324 a         1546 c         1900 g         1863 t
ORIGIN      
        1 tcatggctgt attattttac gcaccatgtt ttacaatgca gttcgtctgc acattactaa
       61 aagctcctgg cttgccgggg gtcgcttaag cggaacagtc cttcaggcca aaaggcagaa
      121 cacgaaaaaa cagaaacact gtaacggtag taaatttcca tcactgtgcc tctgcttttt
      181 tactatcttg atgcggaacg ttttgattct aaaaactttc tgcgtgcacc agcgtggcaa
      241 aaggctgttt cctgccagcg tgtaaaatat gatatgaagt gtttttaaaa actatggccg
      301 ttcaaaaacg tacagcaatg tgcaaaatga acaattccca agaaggcact tttacgtgcc
      361 cttcggggaa ttcgttcatt tgcacatgca atactgttca tttgtaaaca gtatttaaaa
      421 acacctgaca tttccattcc ttacacctgc cgggaaacca acggtaattt tttatttttt
      481 aaaggaagct ctctttctca gcttcggatc cagaattttt ttacggatac gcagatggtt
      541 cggtgttact tccagaagtt catcagtatc gatgaagtcc agggcttctt ccaggcttaa
      601 gatcttaggc ggtgtcagtt tcagagcatc gtcagatccg gaagcacggg tgttggtcag
      661 atgtttgctc ttgcagacat tcagctcgat gtcctctgcc ttaccgttct gtccgatgac
      721 cataccggag taaacttttt caccaggtcc gatgaacagg gtaccacgct cctgagcact
      781 gaacagaccg taagttacgg actcgccatc ctcgaatgcg atcagagaac cctgtttgcg
      841 gtactggata tctcctttat aaggtgcata accgtcaaac atggtattca taataccatt
      901 accctttgta tcggtcataa attcgccacg gtaaccgatc agcccacggg aaggaataca
      961 gaactccagt cttgtgtaac cgctgctggc aacgctcatg ccctgcagtt caccctttct
     1021 cagagacagc ttctggataa cggtaccgga gaattcttcc ggaacatcca cataaaccag
     1081 ctccattggc tctaatttct tgccacgttc atctgtttta tacagaactt ctgccttgct
     1141 tacggcaaat tcataacctt cacgacgcat gttctcgatc agtacggaaa gatgaagctc
     1201 accacggccg gaaaccttga aggattcggt tgtctcggtt tcctcgacac gcaggctgac
     1261 gtctgtgttc aattccttga acagacggtc gcgcaggtgg cgggaagtga taaatttacc
     1321 gtctcttccg gcgagcggac tgtcgtttac gatgaaatgc atggcaatcg tcggctcgga
     1381 gatcttctgg aacggaattg cttccacatg atccggcgag cagagtgtgt caccgatgtg
     1441 cagatcagcg ataccggaga tcgctacgat ggagccgatc gttgcttcgt ttacttctac
     1501 tttattcaga ccgtcaaact catacagttt gctgatcttt acttttttct gtttgtccgg
     1561 atcgtgatgg ttgacaagaa cgcagtcctg gttgacacgg atggttccgt tatctacttt
     1621 accgacaccg atacgtccaa cgtattcatt gtagtcgatg gtactgatca gaacctgagt
     1681 tccggcagtt tcatcaccct gcggagcagg aatataattt aagatcgtct cgaacagcgg
     1741 aaccatatct tttggctcgt catccagact taaagttgca taaccgcctt ttgcagaagc
     1801 ataaacaaac gggcagtcaa gctgttcgtc ggaagcgtcc agatccataa aaagttccag
     1861 aatttcatcg atgacttcat ccggtcttgc ttccggacgg tcgattttgt tgatacagac
     1921 aataacaggc agatccagat ccagtgcctt acgcagaacg aattttgtct gaggcatggc
     1981 accctcgaaa gcatctacca cgaggataac gccgtttacc attttcagta cacgttccac
     2041 ctcaccgccg aagtctgcat gtcccggagt atcaatgata ttgatttttg tgtccttgta
     2101 aaatacggcg gtatttttgg ataaaatggt gataccgcgt tcacgttcaa tatcgttgct
     2161 gtccataaca cgttcggcta cttcctgatt tgctctgaac acaccgctct gcttgagcag
     2221 ctcatctact aatgtagtct tgccgtggtc aacgtgtgcg ataatggcaa tgttgcgtac
     2281 atcttctcta atcataaata atacactctt ccttttgtat atgttgtttt tcgtttttcg
     2341 catctaaacc actacagagt ttatcacatt tttcccagat tacaagggat ttaaaaaaat
     2401 tttgaagaaa aatcggaaaa cagacatatt gaaaccatcc gcagaatata taaaactatg
     2461 cggtgttgca gatgaagaca ccggaaaata caaaaagagc ttcataggga agggagaact
     2521 gttatttatg gcagataaga ttattgaaaa caatcaggta acgatcatag gagaggttgc
     2581 gtcaccattt gtattcagtc acgaagtatt cggggaaggt ttttatatgg ctgacatact
     2641 ggtcaagaga ctgagcgact gttccgactg cattccggtt atgatatcgg aacgcctgat
     2701 tgacgtaagc aaagattatc gaggagaata tgtccagatc aacggtcagt tccgttcata
     2761 caaccggcat gaagaaaaga gaaaccgtct ggtactttct gtatttgcaa gagaagtcag
     2821 ctttgtggaa gaagagaccg ataaagcaaa gacaaatcag atttttctgg atggctatat
     2881 atgcaaagca ccggtttacc ggaaaacacc tcttggaaga gagattgcag atatgctgat
     2941 cgcagtgaac cggccgtatg gaaaatcgga ctatatccca tgcatctgct ggggcagaaa
     3001 tgccagattt gcggcgggat ttgaagtcgg tggccatgta cagatctggg gacgtatcca
     3061 gagccgtgaa tatgtgaaaa aattagaagg agaggaagca cagaaaagag tggcgtatga
     3121 agtgtccgtc agtaaattag agtatatctg agaagcagag atctgcaaag atttgatatt
     3181 cttgcttttt ttgaaaaaat ggtgtataac tgttcaaaga tatgtgaaac attcttgaat
     3241 ttttaacgag gtgcataatg gataaaggtt ctatacatat ttactgtggc gagggaaagg
     3301 ggaaaaccac cgcagcgatc ggacagggga tacaggaagc aagtcagggg agaagcgtta
     3361 tcattataca gttcatgaag gaaagaaatg cagaagcaat tggttttgtg cagcgtctgg
     3421 agccggagat taaactgttc cgttttcaga aggcagaaga ggcatttgaa gatcttccgg
     3481 atgccgaaaa agcagaagag atcaaaaatc tcaaaaatgc actcaatttt gcaagaaaag
     3541 tactggtcac ggaagagtgt gatgtgctga ttctggatga agttctggga cttgtggaag
     3601 caggggtgat cgaagcagaa gaactgcgtg ccctgtttga ggcaaaggga gaatatacca
     3661 gtctgatcct gacagggatc tatccggcga aagaaatctg gcagtatgca gacgaagtga
     3721 cggagatggt cacagcatat aaagcggaca gtagcggaca gtagtggaat cttctacgct
     3781 gagataaaaa agtcaacccg gcagttgaca atatattttg acaatgataa gattataaac
     3841 aaatcagtgt ctgcacggag aagtacgtgc gtaccggttt gagtatcaaa gggggcaaat
     3901 atctgttgac cccgatatca tgtataagtt tgatagaggt tgcgtgtttc aagagtaagt
     3961 catccgatgt attcaggtac aggcgaaggt ggccaaaagg ggaaaacgcc gaaagctgga
     4021 ttgcacctga agcttgcagc ttgggcatgg agctaagaac tctatgactg tcatcgaatg
     4081 acgatggggc gctatctgaa agaatacagt ttttatcatt tgtttattct ttgggacaga
     4141 cctgatcgct cgggtctgtc ccttttatga tacagaagct gttctgttca agtcagatgc
     4201 acctgggatc tgtcatatgg ttccggcagg ctttactgac atttatccga caggatcgct
     4261 gtgcattcta cagtacaagt atcagtttta tagcgtttca gccagttcag attaggagga
     4321 aaaactatgg ctatttttac annnnnnnnn nggcacagat gccatcatca tctgcggaac
     4381 aaccggagaa tcatccacac tgacacatga ggaacatctg gatgtgatcc gctataccgt
     4441 tgaaaaagtg gcaaagagaa tcccggttat cgccggaacc ggttctaatt gtacagcaac
     4501 tgccatctac ctttcccagg aggcagagaa ggcaggagca gatgcccttc ttctggtaac
     4561 accgtattat aataaagcaa cacagaacgg actgatcgca cactatacag caatcgcaga
     4621 gtctgtaaaa ctgccgatcg ttctctataa cgtaccgagc agaaccggct gcaacatcca
     4681 gccgcagaca gccgtatatc ttgcaaaaaa tgtagaaaat attgtggcga tcaaagaagc
     4741 aagcggagat atttcacagg ttgcaactct gatgcagatg gcagacggat gcattgatct
     4801 gtattccgga aacgatgacc agatcgtgcc gatgatgtcc cttggagcaa aaggtgtgat
     4861 ctctgtacnn nnnnnnnnct gcctttgatc catgcactgt tcagcgaagt aaatccgatc
     4921 ccggtcaaaa aagctctcaa tctgatggga aaagaggtcg gaccgcttcg cggaccgctc
     4981 tccgagatgg aagaggaaca tgcagcaaaa ctggcagaag agatgaaaaa gttcggcatc
     5041 aaactggcag aataatggat aatgccagag ttactatgta acggagcaat tcgtaggcat
     5101 tattcttaca tgaatgtaaa gcgaggaaaa agcaatggta aaaatcatta tgcatggctg
     5161 ctgcggacac atgggtcagg tgatctcaca gatcgtaaaa gaggatgcag atgcacagat
     5221 cgtagcaggc attgatccgt ttgataacgg aaaaatggaa tacccggtat acaaaagcct
     5281 ggaggactgc aaagaagaag cagatgttgt aatcgatttc gcatctgcaa aagcagagga
     5341 cgcactgctt gattactgcg gagcacacaa acttccggtt gtagtctgca caacaggact
     5401 ttcagaagaa cagcttcaga aagtggcaga gacagcaaaa aagactgccg tcctgaaatc
     5461 tgcaaatatg tcacttggta tcaacaccct gctgaacctg ttaaagagtg ctgcaaaagt
     5521 actggcagaa gcaggatttg atatggaaat cgtagaaaag catcacaaac acaaactgga
     5581 tgcaccgagc ggcacggctc tggcactggc agacagtatg aatgaggcac tggggaatgc
     5641 ctatcattat acttacgaca gaagcgaccg aagagaagag cgtgatccga aagagatcgg
     5701 tatttccgca gttcgcggcg gtaatatcgt aggtgagcat gaagtgatct ttgccggaac
     5761 agacgaagtg atcgagttta agcatactgc atattccaag gcggttttcg gaaaaggtgc
     5821 cgtacaggca gccaaattcc tggcaggcag agagcctgga ctgtatgata tgcaggatgt
     5881 gatcgcagcg aacgcataaa gaaacagaaa accagaatgt cgttttatca tcttttggat
     5941 ggtgaaacga catttttcgc aaaagcttaa ggggataaaa caatcgtaaa aagaatggag
     6001 gaaattttat gtacagacga tttctgacgg caggagcagc cgcaatgctt gcaaccgcac
     6061 ttttgttatc cggatgcagc aaaaaaacgc agaccgatac cgcaaaacag actgagacgc
     6121 aaacggtaac agagagcagc acggaaagtg aaagttcctc tgaaacggca aaagagacac
     6181 aggcgaaggg cagcacaaaa gaaacagcca aaaagaaagc agaaagtgag actgcaaaag
     6241 agaaagaaag tacatcagaa aaaaacaggg aaacacagac tgacaaggag agtgccggat
     6301 cggaaaaaga gacaaagcag agcaccggaa gcagcgaaac agtgaactct tcggaggtac
     6361 ataccgtgat cgggacgctg gaagagctga gcatggatca ggtagtgttg ctttccgata
     6421 atggaaatga gcttgcgttt tccacggatg atgtaaaaat cgatctgccg tacggtatcc
     6481 ggatcggcaa tctggtttct gtagattata ccggaaagat cacgcagaaa ggtaccaaaa
     6541 aggcaactgc tgtgcgtatt gcgggatcgg ctgatgtttt cgagagcgat ccagaaacag
     6601 aaacgaaggc ggatgcatca aagaagcaga cggtaaaagg aacactggaa aagatttcca
     6661 tgaccggcat gacgattaaa acttctgacg gaaaagaagt gaagttccgg attgtacagg
     6721 ttccgctgta tttagaaaaa ggtctcacaa aaggaatgtc tgtgaagatt acttatcgcg
     6781 gagaactgca aagcgaggac tctggtcagg aaagtgtgga agtggtcaga atccagaatg
     6841 cagactgagg aaaatcgctg gaagaagtgc agaaaacatg catttatgcc ggaatctgag
     6901 aatgcagata tgaaatctga gttataaaaa ctttagaatt gtttagagtt aaactatttt
     6961 tatgtacagt caagtatgct atgataatgc aaagatatca ggattatgtg agtgcagagc
     7021 atggaatgat ccgcagagta tcaaagttaa tggcttttga catcaatatc atgccagagg
     7081 aggaatcagg atgggcgtac agattgtata tacaagccgc accggcaata cggagaaact
     7141 ggcagaagca gtttttgcgg cagtgccggt aaaagaaaaa aatgtaaaac gaatcgagga
     7201 gagaacagaa cgggatgacg gagagatgta tctggttggg ttctggacgg atcgcgggac
     7261 agcgagttcg gagatcttgg atctgcttgg aaagctccac gggaaaaagg ttgcactgtt
     7321 tggaacctgc ggcatgggga atgtgccgga atattatgac cggattttaa acggagttgc
     7381 agcatttatc ccggaggact gtgaatatct gggcggattt ttatgtcagg gcaagatgcc
     7441 gatccaggtt cgcaaacgct atgaggcgat gcagaacact tccaatacac agcaggtgga
     7501 gatgctgctt cacaactttg acgaggcact tcttcatccg gaccgtcagg atctgaaagc
     7561 ggtggcagat tttgtgaaag gattgttctg aaaaaagatg ggaaaatcaa aagaagtaaa
     7621 gacaaatgcc atgcgtatcc ttgagaggaa caa
//
LOCUS       DAWDWH010000089         7631 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_4515_length_7631_cov_16.483501, whole genome shotgun sequence.
ACCESSION   DAWDWH010000089 DAWDWH010000000
VERSION     DAWDWH010000089.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7631)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7631)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7631
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4515_length_7631_cov_16.483501"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1776 a         2103 c         1606 g         2146 t
ORIGIN      
        1 atccgcttca tgcggcggat cacatattca tttctggcac tggaatgcag aagggttgat
       61 gcataagctt cggaaacctc ttctaccgtc tccgccgcca gacgcttctg ttctttctcc
      121 ggaatgttaa accaggtata ttcgctcgcc tccacttttc gtgcgtaaga ctccagcacc
      181 ttatggaaaa tattccccat atccaccggt tggaatacaa acagatctct ttgcgatacc
      241 cgaagaccat aattcagaaa atgtgcatag gagcacgccg aaaatttctc cagccgactg
      301 acactgttga ccagcgtctg tccgtaaagc cgttttgccg tcgtctttga catcggctcc
      361 gtctttccat gcagaaaggc cgcatccagc agctttttcg ccagatcctg atatttttcc
      421 tgtccttcgt accatccaaa cagactggcc gccgtcttct ccacgcttcc aagtgccgct
      481 tttctcagtc cgtccgccag atgcacaatc ccgctctctg ccgttgtgat ccttgcaagc
      541 ggatcttcaa tcagatcttc atcctgaagc gtcagctgcg gaaacatcct taaaatactt
      601 ccgaccacat aggaaggacg cagcactcgt ccgtccagcc ccatccttcc gtaagaaaga
      661 taaagctttg ccgatggttt tgtcagattc agatacagat aaaacttctg gatataactg
      721 ttctcccttg ccgtcggtgc cagttccatg ccaagctcgg ataaggtttc ccggtccata
      781 tcggagagaa tactggtttt ttcttctttt ttcggcaccc agccgtcatt caaccccaga
      841 aaaaacagta ctttgatgtc ctttaacctg gtacgctcga tatcgccgac cagcacacag
      901 tccatcgtcg gagggatcat gccaaccttg gcctcctcaa atccggcatc gaggatttcg
      961 gtatattcct gaaggctcat cggctcatca ccaagcagct ccaccagttt gtcaaacagc
     1021 tcgatcacta tgccgtagat ctgctcgtat tctcttgcaa ggtcataagc ctcttcctgc
     1081 tcaaattttt cccggtaatc ctgcattttc tgctcaatac cataatgcac catcagatca
     1141 taaagtgctt ctgttttctg acgcaccgtc ccgtcctttt ctctgacctg atccacgaat
     1201 gcaagcagcg gcggcaggat cttctctttg aacggttctt cctgttcatc ccacggctct
     1261 tcccagcgtc tgcggctgtt gatcccattt gccagcacat agttttccaa ctggtcgatc
     1321 tcatcctttt ttagccccgc aagcccggtt cgcagataac ggaacatact ctcatagcga
     1381 aagcttttgg ctgcgatctg aagggccgcc cgcagaaatt caaccagcgg atttaagagg
     1441 agcttcctgg tgcggtcgat aaaacacgga atgcctttct gctgcaattc ctttttcata
     1501 tggtcaccaa atgattccat atccccggtg atcacggcaa tatcccggta acgcagattc
     1561 tcttcacgca ccagatgcca gatcgtccgt gccgcaaatt ctgcctctgc tttcgcatct
     1621 cttgccacat gcaaagacag ctcccgctcc agcttctgtg aatcttgggc ttcataaatc
     1681 tgattgcccg gtcggaacag atgtctctcc agaaagcgaa gtgccggtgc acttctgaaa
     1741 cggatgcttc cttcttttcc gagcacttcc ggatattcca gcatacagcc acattctttt
     1801 gccgccccgg ccagctgctg tatcgtcttt ttgctcagat aagcgagttt atgcatggaa
     1861 acctttttca tcggatcttc ccaggcatcc aacgtcaccg tgacatagat tttctccgcc
     1921 gtctgcatca gttccctcag caacttgttc tgaatcggcg taaatccggt aaatccatcc
     1981 agggcgacca cacagccttt taaaatttca gattttttta cgacataaca gagctcttcc
     2041 aggatctctt ccgaagtaat gtactttccc tgaagttctt cctgaaatcc ctcatacagg
     2101 tttcgaatat cctgtaattt ataagataat gcatcttttt tccccgaacg tagctgctca
     2161 tccagttctt ccagtgaaac atcatactgc ttcagctcag aaatgatgga tttgacctgc
     2221 gaaatatagc cgggcttgcg aaaatttcgt ccaagcaccg tcagatcatt tttctgacgg
     2281 gatgccacac gccgcagcag cagattcttt ccggtgtcct ccagaacagt gtgtgtatcc
     2341 accccgactt cctcaaagat cctccatgcc agacgcaaaa agctctgcac atcaatattt
     2401 aaaattcccc tgtttggatg cagttctacg agttcccgct gtgtctgcat ggtaaactgc
     2461 tccggaacga tcacgataaa ctcccggtcc ggctcccgta tggactgctc tatgattttt
     2521 ttataaagtg tatacgattt tccactgccg gatgggccgt agatcaactg caatgacata
     2581 agcatttccc ttcctgtgtc ctgttttgcc tgatgctgca aagtggtccc gcacaaagta
     2641 cagaaccact tttttcacag taagaatgcc ggaagcatcc tgtttcctat cttgcctgaa
     2701 gatgagtcag tctttctttt acctgatcca tcatctggct gtatttctct aattttgcac
     2761 gttcctctgc aactttgctc tccggtgcct tgctcatgaa acgctcattg ctcagcatgc
     2821 cgttgacacg tgccagctct tttgtcagac gctgttcttc tttttgcaga cgctcgatct
     2881 ctttgtcgat gtcaaccagt tctgcaaacg gcatatagat gactgcctcc ggaagcacgg
     2941 cagatactgc atcttcgtcg atgccgctgc ggtctgcctg aaccgtcact tcgcttgcac
     3001 ttccaagggt tgcaaagaat acttttccgt tttcgaacgt atttctcaca ttctcgcttt
     3061 cggatacgac aaatactttt gctttcttgc ttggcggtac gttcatgcct gtacgcacgt
     3121 ttcggatacc gcggactgcc tctttgatca tctcaacggc tgcctcatcc tctgcaaaat
     3181 gccactcttc gatgaactcc ggccatttgg agatcatgat ggattcttcc tctgactgca
     3241 gcgtgcagaa gatctcttct gtgataaacg gcatatacgg atgcagcagt ttcagagcat
     3301 ttaccagaac cgtcttcagt gtccaaagtg ctgctgattt tgtggtatcc ccgtcattgt
     3361 acagacgcgg ttttaccatc tcgatgtacc agtcgcagaa ttcttcccag ataaaatcat
     3421 acactttctg aacggcaata cccagttcat atttatccat gttctcggtt acttcttttg
     3481 ccagtgtatt gactttggag aggatccatt tatcggctgc cgtcagctga tctttcggca
     3541 tttcacccgg aacttctgct ttttcaaggt tcatcatgat aaatctggac gcattccata
     3601 ccttgtttgc gaagttacgg cttgcctcca cacgctccat gtagaaacgc atgtcatttc
     3661 ctggagcatt tccggtgatc agtgtcagac gcagggcatc tgctccgtac tggtcaatga
     3721 tttccagcgg atcaataccg tttcctaaag atttactcat ctttcttccc tgggaatcac
     3781 gcaccagacc atggatcagt acatgatgga acggcacttt tccggtctgc tcgatcgcag
     3841 aaaataccat acggatcacc cagaagaaaa tgatgtcata tccggttacc agcacatcgg
     3901 tcggatagaa gtaatcaaaa tcttcggtct tatccggcca gccaagtgtg gagaacggcc
     3961 acagtgcgga agagaaccag gtatccagtg tgtcttcatc ctgtttaaag tgtgtgcatc
     4021 cgcatttcgg gcagaccttc ggcatttctt ttgcaacgac cacttcgccg cactcgtcac
     4081 agtaatatgc cgggatacga tgtccccacc agagctgtct ggaaatacac cagtcacgga
     4141 tgttctccag ccagtgcaga tagatcttgt caaaacgatc cggtacaaaa ttcagctcgt
     4201 tctttttcag agcttcgacc gccggtttcg ccatctcctg catctttaca aaccactgcg
     4261 gtttgatcat cggctcaacg gttgtcttac agcggtcgtg tgtgccgacg ctgtgtgtat
     4321 gcggaacaac ttttaccaga agtcccagtg catccaggtc tgctaccatg gcttttcttg
     4381 cctcataacg atccatgcct gcatatttgc cgcccagttc attgatggtc gcatcgtcat
     4441 tcatgatatt gatctccggc agattatgtc tcttgccaac ctcaaagtcg ttcgggtcgt
     4501 gtgccggtgt gatctttacg catccggttc caaattcttt atctacgtaa gaatccgcaa
     4561 tcaccgggat ctcacggtct gtcagcggca gcttcagcat cttgccgacc agatcctggt
     4621 atctttcatc ttccgggttt acggcaacgg ccgtatctcc aagcagtgtt tccggacgag
     4681 tggtcgcgat ctccacgaaa cgtccttctt ctccgacgat cgggtaattg atatgccaga
     4741 agaatccgtc ctgatctaca tgttctacct ctgcatctga aatggaagtt ttacataccg
     4801 gacaccagtt gatgatcctg gagcctttgt agatataacc tttgttatat aattttacga
     4861 aaacttcttc aactgctttg gaacatccct catccattgt gaaacgttct ctgtcccagt
     4921 cagcagaaga acccagtttt ttcagctgat ttacgatgcg tcccccgtac tcctctttcc
     4981 actcccaggc gtgttttaag aattcttcac gtccgatatc gtttttatca atgccctgct
     5041 ctttgagttt ctggatgacc tttacttccg tagcgatcgc cgcgtggtcg gttcccggct
     5101 gccacagtgc attatagccc tgcattctct tatagcggat caggatatcc tgcatcgtgt
     5161 tgtccagtgc atgtcccata tgcagctgac cggttacgtt tggcggcggc ataacgatcg
     5221 taaacggttt cttgctgcgg tcgacttccg catggaaata tttcttatcc agccagttct
     5281 gataaatacg atcctcgatt cccttcggat cataagtctt tgctaattct ttgctcatgt
     5341 tgtcctcctg ctgttgtttg ttctgcacat taaatcaaat acactccaaa cggaatgttt
     5401 caacgaaaaa agccctctgc atcatccccg gaaacggaga ttctgcaaag ggcgtcattt
     5461 acgcggtacc accttttttc atatacatca cagacggatg catacctcat ttccctttaa
     5521 cgtcgggtca cgtgaaagct tactgtcctg tccgcgtata cttatctgca taccgcctgt
     5581 cacagaactc gttctgccct ccggctccaa agctaccttc catccacgca tccttaggtt
     5641 tccttccagc ctgcgaaaaa ccctctctga aagagctgtg tatgtactcc tcttcttcct
     5701 cgcctttgtc catgttaatc atagcgaagg cagacggatt tgtcaagaga tgactgtgga
     5761 ttttgttccg gtgaaattga tgcgaaaata ctattgagcc ggccagcaat ccatgataca
     5821 cttgacacac agaagatttt gcaatatttc gcttttcttt ctggtgctaa tgatgaaagg
     5881 agttttttta tgcctgtatt tgatttcaat aacaccccgg aaccggatgc cgttccggaa
     5941 tgtacttata ccacctttgc ttccgacgaa aatgtttctt ccgcagacaa accgcttctt
     6001 gtccttgaca acagcaaaaa agcctggaaa catcactcct gcggaacgtt taccaatcag
     6061 aacaagcgta ccgcttttga atttaaagaa gatgatggtt ctactgtaat cgctgatatc
     6121 aaaaaagtgg atgcccgctt tgtaagcctg ctccgctggc tcggtgaaaa tcacattccg
     6181 gttcgtttat ccggtgaaaa ccgggagggc ggctatatgg tttaccgtat ccgcgagact
     6241 gctttcggcg gtggcaccaa gctttctgca gaggatggtt tccttcagtt catgattgaa
     6301 cgtctgcttg caagcaatgc tccgcttctg gaagaatgtg aagacgaaga tctggaggaa
     6361 gtcggcgatg agatgaaact gaccagcctt cagagcatca cagactttat gatctgtgcc
     6421 ggccgcaccc tgccggataa tatccgcctg tgggcaagac gcaaccttgc cgttgcacgt
     6481 tcccatgaag tttcccctga agaacgccgc catgcccagc gtgccctctc catcatgatg
     6541 aacattcagt ggaagagcaa ttacttcgaa gccatcgatc cggttgctgc acgccgtatt
     6601 ctcgatgaag aactttacgg tatggaacgc gtcaaacagc gtatcatgga gacgatcatc
     6661 cagatcaacc gcacgcatac cctgccggca tatggtctgc tgctggtcgg ccctgccgga
     6721 accggtaaat ctcagattgc ctatgccgtt gcacgtatcc tgcgtcttcc ttggactacg
     6781 cttgacatga gctccatcaa cgatccggaa cagctgaccg gaagctcccg tgtctatgca
     6841 aacgcaaaac ccggaatcat tatggaggct ttctcagtat ccggagagtc caatctggtc
     6901 ttcatcatca acgagctgga taaggcagcc tccggcaagg gaaacggcaa tccggcagac
     6961 gtgcttctga ctttgcttga caacctcggc tttaccgata actatatgga atgtatgatt
     7021 ccaacgaccg gcgtttatcc tatcgccaca gccaacaaca aagagcagat cagtgcacct
     7081 ctgatgtcac gttttgccgt gatcgacatc ccggattaca caccggatga gaaaaagatc
     7141 atcttctcca aatttgcact gccgaagatt ctcaaacgta tgagcctcaa agagaatgag
     7201 tgcaagatga cagacgaagc actggatgct gtaattgaac tgttctccaa cacctccgga
     7261 atccgtgatc tggaacaggc cgccgaacat atcgcagcaa atgcactgta tcagatcgaa
     7321 gttaaccgtg tttccgaagt tgtgtttacc gcagagatgg tacgcagcct cttacgcgac
     7381 taagcataca tggaagattt atgcatgtgc aaatgatctg tgcagaaggt ttgtaaatgt
     7441 gcagacgaac gccataaaat agttttgtaa aaatagtacg ccatgatgat tcgaaaacaa
     7501 tctcctgaca cgctgcgttt ccttttcggt tcgctcgtcg gttagccgcc cttcgcctaa
     7561 actcgcctct tcgagtctca gacagcggct ccttggggcg gcagcatcgc aaacctcacg
     7621 acggaagcct t
//
LOCUS       DAWDWH010000090         7582 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_4545_length_7582_cov_13.836057, whole genome shotgun sequence.
ACCESSION   DAWDWH010000090 DAWDWH010000000
VERSION     DAWDWH010000090.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7582)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7582)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7582
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4545_length_7582_cov_13.836057"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1856 a         1855 c         1611 g         2260 t
ORIGIN      
        1 aaagataagt atcccatatg ttccggtaaa tttcttttgc atcttttctc tgatcaatat
       61 gttttacaat ggtaggagcc agatcataat attcataaac gatcgcttct ccgttttcct
      121 gctccatcaa ataatggtcg cggtaatttc ggaaaaggtt cagctcataa caatcatccg
      181 gtttgccaaa tgttctgcac actgctgtgg tgatgtaaca gaatttccgt ttaaatccag
      241 aattgatctg cttaaaagta gaagctttga gattggtttt cggaaaatgt gctttccagg
      301 cttccagaat ctttttggaa aaaagattcg caggtgcttt gttcatctca agaacagccg
      361 gaagcgtata gattaccagg cacatattca ggtttgcagc cagtttctct tttgcatttt
      421 tcttttcctc ttcggcaatt cgcttttcag cagaatttgc aagctcttct gccatattgg
      481 tcagaaactg ttctttatca ataacctgaa gatagccatc ctcgactgct tttaatgtcg
      541 ctttgtgttt gttgcagtag cgtttaaatg aatccggata gcgttcgaac gtgaaatttt
      601 ccatgacatg ttcttgttca tccagcaatg ccggcatccc tgcaaaagct atggtataat
      661 tactgctcat atatcacaat cctcattaaa cggacttttt atccgccttg tcgttttatt
      721 catccagatt ggtatatacc gcctgaacat catcgtcttc ttcgagcaga tccagggtac
      781 gctgaaggtt cttcagagca gtctcatcat caagagcaac ataattctga gggatcatgg
      841 tctcttctgc ctgagccatc gggatctttt tggcttcgag agtttctctt acggcctcca
      901 gatcatctgg tgatgtcagg atctcaaagc tgtcttcttc ttcggaaaaa tcctctgcac
      961 ctgcatccag agccagcatc atcaggtcgt ctgcatccat atcacattct tctttatcga
     1021 tcacgatctg acctttgcgg tcgaacatgt aagaaacaca gccctgtgtt ccgatgcttc
     1081 cgtttccttt tgtaaatgca cttcttacat tggctgcagt acggtttttg ttgtctgtca
     1141 gtgcgtccac gataatcgcg attccgcaag gaccatatcc ttcataggaa acatactcgt
     1201 aattgacaga gtttgcatct ccggctgctt ttttgatacc acggtcgatc gtatcgttcg
     1261 gcatgttgtt tgcttttgct tttgcgacaa cagcggcaag tttgctgttg tttgccggat
     1321 ctggtccgcc ttcttttacg gcaactgcga tctcacggcc gataatggta aagatctttc
     1381 ccttcgcagc atcgtttttc tcttttttgt gctttatgtt tgcaaatttt gaatgtcctg
     1441 acatttcgaa atcttccttt ctcttccaag tatctgtttc ttcattctgc cgcttaacgg
     1501 cctgatttcc ctgcacagga ggcaggcata tccattatga taacaccaca ctgaaaaata
     1561 gtcaagttcc caggatcaat cagctatgta gatccagact gactgcgaga gcctgattta
     1621 cgcggtccag gatttccgta tccagatggc atactttatc tttcagtcgt tttttgtcga
     1681 tggtacgcag ctgttccaga aggattacag aatcacgtac catgttatat cttgaagatt
     1741 caatctccac atgtgtcggc agctttgctt tgttcatttt ggaggtgatc gctgcacaga
     1801 tcaccgttgg actgtgccgg tttcccgtat cattctgaat gatcagaacc ggacgtacac
     1861 cgccctgttc cgagccgatg accggtctta agtccgcata aaaaatatcc cctcgtcgaa
     1921 tcaacacaca cttcacactc cgcttctggt tttaagctta gtatttccac atttttctgg
     1981 tgtatgcagt tcgccttaag gcaggatcag ttggtaccgg aacagtcacg taccagatag
     2041 acttcgtgat ttctggtgta gatacgcggt acacgtttgc cgatatcaca gataaattca
     2101 taagaaaagc gaccgcaaag atcaccaagt tcgtctacgg tgatctcttc gtctccctgt
     2161 ctgccgacta aaattacttc atccagttct tttgcctgcg gaatgtccgt cacatcgacc
     2221 ataaactgat ccatgcagat ccttccgagg atttttgcac gtttcccgtg tatcagaaca
     2281 tatcccttat tggaaagcat ccgcggataa ccatccccat agccgaccgg gatcgtagcg
     2341 atccgcatcg gatgcggagc ggtaaaggtt ccgccgtaac tgatctcggt gcccgtttca
     2401 atctctttga tatacacgat gcggctgtga atggacattg caggcgtcag tttcatagaa
     2461 tctctggaaa cttcagcgga tggatagatg ccgtaaatac tgatgccggc acgcaccaga
     2521 tttaagttca tatctgcaag ctccatcaga gcggcactgt ttgatacgtg ttttaatgga
     2581 atcgtaatgc ctgcctcttc cagtctgtgt gcaaaatgca tataacgttc aaattgtctg
     2641 tttgtagccg ttttatccag ttcatctgct ttcgcaaagt gcgtaaacat gccttctatt
     2701 tcaattcccg gtaaggatgc gattttacgg attgtttcta tgctctcttc ggtatcagaa
     2761 aaaccgatgc ggctcattcc cgtatccagt ccaatatgta tagaaagtgt tttctttaaa
     2821 tgaacggctg cttcagacag ttcctttgcc atatccagtt tgaacacaat cggtcggatc
     2881 tcgtaacgga tcagatcttc gtagtcttct tcgaatgtat agcccaggat cagcaccggt
     2941 tttgtgatct ggtttttccg caggatcatc gcttctgtca tcgttgcgac ggcaaatccc
     3001 cagatataat cttccggctg tatcatctgt gcgatcggca ctgcaccgtg accgtaacca
     3061 tctgttttta ttacagcaac cattttggtt tccgggtgga tcgcttcgtg catgatccga
     3121 aaattttctg caatggcatc catgtcgatc tcggcacaga cacggctgta tttactgctc
     3181 atcatgtttc aagtccttcc tgtattgttt tttgtaaccg ttgctttcgt tacttacatt
     3241 tatttttatc gtctgcatct gccagaacca ggatcagtcc ttccaccaga tcatttgctg
     3301 tcatacttcg ctttccgtat ttttgtgcgg ccgcatcgcc ggcacatcca tgcagaaaaa
     3361 cagcaagttt tgcagcttca aataacgtca tcccctgtgc ggtaagcccg gcaaatacgc
     3421 cggtcagcac atcgccggaa ccggcagttg ccatgccgct gttcccggaa gtgttgatgt
     3481 aggattcact gccgttgctg acaacggttc cggcatcctt taagacacag atcacatgtt
     3541 gttctttcgc aaagatttcg gcacttgcga tcagatcctc tgaaatatcg gaaatggttg
     3601 atttgcacag tcttgccatc tccccaagat gtggtgtcac agctaccggc atggaatatc
     3661 catcaaggga aaggttatgg cgtgccacaa gattcaaagc atcggcatca agaatgaccg
     3721 gaatatccgc atgttccagt acataattca ggatgtcaag cttttgtgct tccatgccga
     3781 tgccgggacc tactgcaacc acatcagccc acgcaagcag gttttcaagt tctctcttcc
     3841 agttggaatg gatgcagctt accactgctt ccgggataga ctgaaaaagg gcggactgat
     3901 tgtgttcgtc tgtgtagatg cgaacataac cggctccgct tcgatacgct gcacgtgcac
     3961 ttaagattgc agcaccgcac atgtcccggc tcccggcgat cagaagaact ttgccgcagg
     4021 aacctttgtt ttctgtggca agtcgctctg gcagccgcct taaatccata ctgtcatagc
     4081 agacgtaaga tttccggcat ttcggaaggc tttcttttgt aataccgatc tgacgtacac
     4141 gtacctcttt ggtacaggca cgcccttttc cgatataatg ccccggttgc tcaaaagcaa
     4201 atgtcactgt caggtcagcc tttacggcaa tcccccgtat cacacccgtg tttccgtcaa
     4261 tgccggaagg aatatcgaca gacagtacaa aagcaggact ttcattgatc cgcccgattg
     4321 cgtcccggta gctgccttcg atgtcacgcg aaagaccgat tccaaacagt gcatctacta
     4381 taacagtata ttcatttatt tccgggttac tacaaaaagt tatcccatat ttctcacaga
     4441 tttctttctg atggcgtgtc tgatctgtgc aggactgctc ttttccgaca aaccatatgg
     4501 aaaccggtac ctgatccaga aacaaaagtc tggcgatggc aaatccgtct ccgccgttat
     4561 tgcctgaacc acagatgacc agaacgcggt caagtgcagc accgcttcgg tacagttcat
     4621 cgacacacgc aagtgccgca cgctccatca gtacacagga tggaacgccg aacccttcta
     4681 tggtattctg gtcacatgtt ttcatgtctt cttttgataa caagatcctc atatgcgata
     4741 tacctcctgt ctgcccgtcc aaatggaata tacagttttc tgttttcttt ctctgcgttc
     4801 tttttattta tgttctctgc tgtagcggct gatattgtaa gacagctgca tcaggctttc
     4861 cgaaagatgt acgttttcga tgatcacatc ttccggcaga tgaagatccg tatgggcaaa
     4921 attccagatt gcacggatgc cgtgatccac cagaaagtct gccatttcct ttgcatactg
     4981 cttcggaagt gtcagggctg cgatctgaat gtcatgctcc tgcaaaaaag caggaagctc
     5041 gtctgtcata cggatcacaa ttcctctgac tgcaacgcct ttcagctttg gatttacatc
     5101 gaacagccct ttgaccagaa aaccgttttt ttcaaatttt acataatttg caagagcctg
     5161 tccgagattt ccggcaccga tgatgatcat attgtaatcc tgatccagac ccaggatctt
     5221 cccgatctct gtatgtaaat gttttacatt ataaccatat ccctgctggc cgaatccacc
     5281 gaaattattg agatcctgcc ggatctggga tgctgttacc tgcatgcgtt cgctcagctc
     5341 gccggatgag atccgctccg taccggattc cagcagctca ccgagataac ggtaataccg
     5401 tggcagtctt ttgatgaccg ctcttgaaat ttctttctct tccacttgca tacctccctg
     5461 tcaaatgctg ttgtatagta acgtaaaaga cgggcacaca agatccctgc catgtccgcc
     5521 gcaggttttg tgtaccctct ttgattttta gagctgtacg gttacaaaaa tatcatagat
     5581 tgatttgatc gcacgctcga aatcctgatt gctcacaccg atgatgatgt tcatctcact
     5641 ggaaccctga tcgatcatct tgacattgat acttgcatgt gccagtgcgg aaaaaatacg
     5701 tcccgctgtt ccacgctgag atttcatgcc acgacctacg actgcgatca gtgccaggtc
     5761 agattccaga ttgacagaat ccggattgac agcacggtgg ataccggcca gtaccttctg
     5821 ctcggtctct tcgaattcgg actgatgtac gaataccgtc atggtatcga ttccggaagg
     5881 catatgctcg atggagatac cattgtcttc gaatacggtc aggactttgc ggcagaatcc
     5941 gatttcggag ttcatcatat ctttttcgat gttgatggct gcaaatcctt tctttccggc
     6001 aataccggtg atcgtataac gcggttttct gcaggtactt tcaacgatca gcgtaccagg
     6061 atcctgagga cggttggtat tgcggatgtt gatcgggata ccttcctgtc ttaccgggaa
     6121 gatggcttct tcatgaagca cggtagcacc catgtatgcc agctcgcgaa gctctacata
     6181 agtgatggta tcgatcactt tcggattttt gatgatacgt ggatctgtga tcaggaaacc
     6241 ggatacatcg gtccagttct catacagatc agcatggact gcttttgcga tcagagaacc
     6301 tgtgatatcg gatccgcctc tggagaaggt tgttaccttt ccttctgcgg ttgcaccata
     6361 aaatccagga atgactgcat tgtcacattc tgccaggcgt gcagaaagct tttcattggt
     6421 tgtttctgca tcaaatgtgc cgtcttcatg gaaaacgatc acttcagctg catcgataaa
     6481 ttcatatccc aggtaatttg ccatgatgat accgtttaag tattctcctc tggaagcagc
     6541 ataattggaa cctgccagtt ttttaaagtt ttcggagatc acttcaaact gatcatccag
     6601 agacagggaa agtccgagac cgtttatgat ctccatgtat ctttcacgga tcgcagcaat
     6661 cgaagctgtg atgtcttctt ttttctctgc ttttgcgtag catgcataaa gcatatccgt
     6721 taccttgata tctccatcaa accgtttgcc cggagcagac ggaacaacat attttcttcc
     6781 tttatcagca cggatgatat ctcctacttt tttaaactgt tctgcacttg caagagaact
     6841 tccaccaaat tttactactt ttctcatgct ttttattcct cctaaattcc gcatctgtct
     6901 gtcacgcaaa tatacgctgt ctttaatcat accgcaaaat atgcggttcg tccagagcat
     6961 tttttgaaat ttgctggtgc aaaaagcaga aatatgctat gataatacga gtcagggtct
     7021 tcgcagacac aaattatttt tgtaaaatat atacacttgg aggaatttat gattttagcc
     7081 tgtcagaata ttaataaggc atttggcaca aatgtgatct tgaaagatgc ctcatttcat
     7141 atagaagaaa aagaaaaagc agccattgtc ggcatcaacg gtgccggaaa atctacactg
     7201 ctcaagatca tcatgaagca gatcccggca gattccggag aagtgatcct ggcaaaagac
     7261 cgcacgatcg gttaccttgc ccagcacgaa gcggtatctt ccggaaacac gatctacgaa
     7321 gagcttctgg aggtaaagca ggatattttt gagctggagc gtcatatccg cacgctggaa
     7381 cttcagatga aatcccagag cggcggggag ctggaacaga cgctggagct ttataatcgt
     7441 ctgaacacgg aatttgaaca gaaaaacggc tatgcctgca aaagtgagat cgtcggtgtg
     7501 ttaaaagggc ttggatttac ggaagatgaa ttttccaagc aggtcgatac cctctccggc
     7561 ggacagaaaa caagggttgc cc
//
LOCUS       DAWDWH010000091         7564 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_4555_length_7564_cov_12.384206, whole genome shotgun sequence.
ACCESSION   DAWDWH010000091 DAWDWH010000000
VERSION     DAWDWH010000091.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7564)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7564
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4555_length_7564_cov_12.384206"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3079..3088
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1771 a         1964 c         1520 g         2299 t
ORIGIN      
        1 gggtgcgttc tgccacattg accatgtatt cacccagttt gtttttcgcc ttgtggatac
       61 ccggtgtgtc cagaaataca atctgtcctt tttcgtctgt atagacggtc tggatccggt
      121 ttctcgttgt ctgcggtttg ttggacgtga tcgcgatctt ctgcccgatg aggtggttca
      181 tgagtgtgga ttttcctacg tttggtctgc cgatcagggt cgcaaaaccg gatttgtaac
      241 ttttctctgc catctgtata tgatcctcct taaagtggtc cgagatttct aaacatctct
      301 ttctcttttt ccagtgcatc ttcgtttccg agcacgcaga tgcagtcttc ttccaggatt
      361 gcacgcatca gcggtgccag ggcacggata tcttcctgtg tcgcatagag tacctggtca
      421 cgctctttct gaaggtcgct ttcagtcaca tgtccgaaat aagcagcatc agaacgtgct
      481 ccctgtgctc ttgctgtgag tggcgtatcc atctcactga tcgttccgat cacgtatttt
      541 gtcatatcac gctcttcgac gttgaaatgt tccacgtatt ccggcacgcc ttcgtagatc
      601 tcgttggtct gacgcaggtt cggatcacgg taagaagcaa aataggaatt gccatttctg
      661 ccgaatccac tcatacagcc atacgccccg cctttgacac gcacattcac ccagagatat
      721 tcatagctca tgatcacctt caggatccgc agtgccccgg tataatcata gcctgcctcg
      781 cggtaatttc cggcacgtgt cacatactgg atcttggcgg aggtctggaa gccttcgttc
      841 agctttccgt atgcaagggt ttcttctgaa ggtgttctct tatccaacga aagtttttcg
      901 cataatcctg tcactttctc ttccagtccg gcaagcccct tctcttccgc tgttacggaa
      961 aataccatgg catcctgtcg gaaaatcacg ttcatcagct ctttcaattt actcttcaga
     1021 ttctcttttt tcgtttcaaa atttgcatcg aggtcatcga gcatgcgata gtagtcaatc
     1081 ccgctgctgc gatcactgaa cgcagctgtc tcggagaaat aagccatggc acgggttgcc
     1141 gctgccgaat gtcccgccgt tgtcagccgc atctgtgtcc ttgatttctg ctcggaaatg
     1201 atctctttta agcgtttctc atcgtccagt ttggatgtga ataaaatctc ctcgatcaca
     1261 tcaaacacaa agtcgatttt atcataaagt gtgcgggctg atacggtaaa catcggatac
     1321 atcttctgtg tgtccttttc tggaaatatg accatcccgg taccgatacc tccggtctgc
     1381 atattgatct cattgaacag atctccgtaa gcataatgct cggtgtcgat catgcccagc
     1441 acatttttca gaaggccgac ataaggcaga tcctcttttc tgacaacgga cagatcaaaa
     1501 taaagggtca gataagcgat gccgttcgtg ttcacatcat gccacagaac tttggtgttg
     1561 ccaaaagaaa gctctctgtt gcagatcggt ctgcatttct ttgtgatatc ctccctggta
     1621 agcatcggga ttttctccag ctcttcctgt gtggacggtg tctcctggaa ttcctgcagt
     1681 tttgctgttt tttctaccag ctcctttacc tgttcttcac tgagacctgc cttaaaatct
     1741 gccagtttct ttgccgtctc tgcctccagt tttgcagtca gtcccttttc cggtgcaacg
     1801 acaacaaccg ccacatgtgt gttatccagc agatatttct gcaccagttt ctcgtaataa
     1861 tcactctcta ctgcatcacg gagttcctta taaatatcca gctgtttgac ataggcaaac
     1921 ggatcaccct cgccgtacag ccagctgtcc ataatgtcga ttccgtacat caggcccttc
     1981 ggataagagc cgtaatctgc ctcgcgaaaa cggaactcgg aagaattgat acctgccagc
     2041 agtgcttttt tatcgatgcc gtttttcacc tgctcttcca gtaccttgcg gatcactgcc
     2101 aggaactttt ctttgtcacc gacatttgca tttttagcga ctaccgaaag ggatggctgc
     2161 tgaatgccgc tctcaaaaga gcctaggata tcttttccaa tcccggcatc caaaagagcc
     2221 tgtttaagcg gtgccccagg tgctgacaaa agtgcatatt ccaggattgc catggcagtc
     2281 acaagacgta catcgctgct tgtgccaacg gacacattcc aggaaagata ggtgttgtct
     2341 tcttccgatt cggatgtgga aatagaatat tttttataga tctcatgcgg agcatcgaac
     2401 gctttctgca tactgatctt tgaatctacc tctttttctc tgtatttaga gagatattcc
     2461 tgatccagcc aggtgaggcg ttcctcaaca tccatatttc catagagata gatgtaagca
     2521 ttggacggat gataatattt tccatggaaa gccagaaaat cttcgtattt cagatccggg
     2581 atacactgcg gatctcctcc ggactcaaat ccatacggtg tatccggata aagtgcattt
     2641 aaaatttcgc gatcaagcac atcttccggt gaagaataag ctcctttcat ctcgttgtat
     2701 accacaccat tgtaggtgat ctcatcctcc ggattttcca gatgataatt ccagccttcc
     2761 tgacggaaaa tctcttcttt tttataaata ttggtatgaa aaacggcatc catatagaca
     2821 tccatcaggt ttgcaaaatc tttgtcattg cagcttgcaa tcggatacat ggtcttatcc
     2881 ggataggtca tcgcattcag aaaggtattg agcgaacctt ttaccagctc cacaaacgga
     2941 tctttggacg gaaacttttc gcttccacac agcgaacagt gttccagaat atgtgctacc
     3001 ccggtgctgt ctaccggagg tgtacgaaat gtaatatgaa atactttatt atcgtcatca
     3061 tttgcaatga ccatgatgnn nnnnnnnngc tttttctgtg acgcagcaga tagccgactg
     3121 actgaatatc ggaaagcccc tcttccctta aaagttcgta cgcctgtaat tcttttattg
     3181 ccatgaaaaa acctcttttc tttttattta ttcataaaaa ctgcagccct ttctgccata
     3241 ttcttttaca taatagagtg cctggtctgc atctttaaaa atactctctc ctttattcgg
     3301 gcggtctgag tttctgtccc gtcaaaatat cttttcaaaa tatccagagc tttctctctg
     3361 ctctgttttg tttctgcttc ttcaaaataa gcatccatat atttactttg cccggtcatc
     3421 gcataaagcc tgacctgctc cgtcagataa tcggatccgt tctgcagctg cttcgctgca
     3481 tttccacatg caatatactg gtttgtcgtc gtctgaagta catgaaactc ctttcggtca
     3541 ataaaggaaa ttatcttctg aattctctca tgtacaaacc cctgttatat caacggaaaa
     3601 agtgcgatcc catacgtgac accgcacttt tcctgttgga ttgcctgtta tttatttacg
     3661 cacaattttc tgaccatatc tgccgggatc attgtacatg ccagcagaac aactgcaatc
     3721 cagcctttga ttccaaacgg aacacagctg aacatttctc caatccactg gaacggggcc
     3781 agcggaacga tcgccgcatt tacgagcaat gcctgcacga ttataataat cagaaatact
     3841 ttaataaaat ctttattctc attcagaccg gcaaaaattg caaatccatc gtcacgcaca
     3901 ttaaagccgt tgaacaaagc tgccacaata aacagtacaa aataacccgt caggtgctgt
     3961 tcctcatttg caaacaaatt tacaaagaac ggcactttca gatagataaa gcttaccacg
     4021 gtaagccaga ttcccattgt gaggatctgt cccatcattt ttttgcttac aatactttca
     4081 tcacgttttc gcggtgcttc tgccatatat tcttcttttg ccggttcatt gccaagcatc
     4141 atcgctccca gtccgtccat gaccagattt acaaagagaa gatgtgttac tttcagtggt
     4201 tcatccacac cgaaaaacgg tgcgatcgca ctgaccacaa cggctgccac gttgatcacc
     4261 agctgaaatt tacagaattt cagaatgtta tggtagatgg ttcttccata gaggatggca
     4321 tctttgatgg atttaaaatt atcatccaga ataacgatct tacctgcttc ttttgctgct
     4381 tcggttccgc ttcccatggc aaaaccaaca tctgcacgtt tcagtgccgg tgagtcgttg
     4441 acaccgtcac ctgtcatgcc gactaccagg ttcatctcct ggcacaggcg taccatacgg
     4501 gacttgtcgg tcggcagtgc tcttgcgatc acacggatat tcggtatgat cttcttcact
     4561 tcttcatcac tcatctcatt gagctgtgcg gaggaaagtg cacggtcgct gcctcctttt
     4621 aacagtcctg catcttttgc gatcgctacc gctgtctcaa ggcggtcacc tgtgatcata
     4681 acgacctgaa taccggctcc ctgtacttcc cggatcgctt cttttgcttc cggacgtaca
     4741 tcgtcacgaa tgcccaccag accaatgatc acgatatcat cattgattga attttcaacc
     4801 atgtcttttt ctgaataacc aaaggccagc acacgcattg cttttgctgc cagttcatcg
     4861 atcttgcggt tcaggatggc gttgtccatg gtacagatat tgccgtctgc atccagatat
     4921 ttcactgcat ttgccagaag tttttccggt gcccctttat agaaagtttt accggtgctg
     4981 tcgatgcgtg cctgactgaa tttatttgca gagttgaaag actgctgttt tgttatttta
     5041 tattcgctgc tgttctccag cgtcttgaat gtctcctcac ccaaaaactt catcagtgcc
     5101 tgatcggttg catttccgcc gatgacttta tgggatgcgt caaacatgga ctgtgtattc
     5161 ttaccgatgg caagatccac aagacctttt accttgctgt gtttactcag ctgtgcaata
     5221 tcaatggaat tgccgtcgcc ggtaaagaaa tcgaccactt caagcatacc ttttgtgatc
     5281 gttcctgtct tatcactgaa gagaatgttt aaagaacctg ctgtctcgat accttctgct
     5341 tttcttacca ggacattgtg gtcaagcatt ttgcttgtat tttgcatcag tacgagggag
     5401 atcatcagcg gaagtccttc cggaactgca cagacgatga tgacaattgc cagagaaaga
     5461 gcccttacaa catctttgac aaccggttca atccccattg caaagtactg ggaaactccg
     5521 cccgcctgca taacaaagta gaccaggtaa agtacggcga tcaataccgc accgatataa
     5581 ccaaacgtgg aaatctgttt tgcaagtttg gcaagtttta ctttcaaagg agaatccggc
     5641 tcgtcttcct gcatctcttc tgccattttt cccatcatgg tcttcagtcc gaccttgcgt
     5701 acatcaagaa taccttcgcc gtcaaatatc actgctccgc ggaacaggga gtgtgcatcg
     5761 acaaaggtat cgcctgtgat gtcatcttcc agctgaaaat cttctgatgc tgctgttttt
     5821 ttacattctt ctgcctcgcc gttcagtgcg gagttgtcca cacgcagtgc cccgtcgatc
     5881 agtacaccgt ctgccgggat tttatcaccg gactgcagca ggactttgtc accgactaca
     5941 acatcatcaa catcgatcac cgtgatcacg ccgttcctgt gaactttaca cttgtctttc
     6001 tctgtattgt ctttcagttc acggtacttg gtatcgcttg caacaccggt ctttgcagat
     6061 acggttgcca cgatcagcac tgcaacgatc gttccgagcg gctcgtaaat ctccgcatac
     6121 ccaaagaaga acatcacgat catcagtgct gcgatcgcca gcaggatctt gatcatcgga
     6181 tcactgaacg tctctttgaa ttcttcccag aacgtggtcg gctcggaatc cgggatctcg
     6241 ttggaaccaa atttacttct ggactcttcg acctgtttgt ctgtcaaacc attatacttc
     6301 atctttttgt ctcctgtttt tatttttctg cttataagca gggagaaact atatgaatca
     6361 cttcatctca gatatagtct ctcccatatc gcaccatgtc ataccggtac gctcttattt
     6421 ttgaaatctt gctgccatct caccgatgcc gccgtccctt gttccctgtc caatggcacc
     6481 gaatttccac tcaccgttgt agcgatagat ctcaccaaag atcatcgccg tcattccgga
     6541 gtagtcctct gtcaggttgt actttaagat ctcctggtca ttttttccgt ctaccaggcg
     6601 gataaacgca ttctcgatca gtccgaaatg ctgctttctc tgctgtgcct gatagatcgt
     6661 tacaacgatc acgatcttgt catactcaga cggcacacgg tccaggtcga tcacgatctg
     6721 ctcgtcatcg ccttctcctt cgccggtcag gttatcaccc atgtgacgga cggttccgga
     6781 tttgtgtgtc agattgttat agtataccag atcatctctt cctgccattc tgccgttctg
     6841 cagtaagatt gcagaagcat cgcagtcgat cggtgccggt ttcagtgcaa aaaatcctct
     6901 ggatcttttg acttcatccc atccaagtcc tactacgact ctggataacc cctttttctc
     6961 tttggacaga ttcactttct gtcctttctg taaactgata gacataatca tgcccccttt
     7021 tccaggtttt taagcattgc tcaaattcat tttatctgcc tgtgctactt atgcgacttc
     7081 gattccataa tgtccgcaca gtgctgccag accgccctgg aatccgctgc cgatggcgtt
     7141 gaatttccat tctccgttgt gcttgtacag ctcgcctacc acaacggctg tctcaatgga
     7201 gaaatcctct ccgaggtcaa aatggatcag ttcttttccg gttgcatcat ccacgatacg
     7261 gataaatgcg ttggatacct gtccgaaatt ctgtcttctg acatctgcat cgtagatcgt
     7321 tacggtaaat gcgatacgtt cgatgtcgga tggaacctgt gtcagatcga ccatgatctg
     7381 ttcgtcatcg ccttcgccgg aaccggtcag attgtcgccc atatgtttga cacatccggt
     7441 cggatgttcc agatttccgt aaaagataaa ttctttttcg gtcgggcatt tgccgttgga
     7501 accgcacata aatgctgctg catccaggtc aaaatctgct cctgtatcaa atgcgttcac
     7561 atcc
//
LOCUS       DAWDWH010000092         7400 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_4676_length_7400_cov_8.811981, whole genome shotgun sequence.
ACCESSION   DAWDWH010000092 DAWDWH010000000
VERSION     DAWDWH010000092.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7400)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4676_length_7400_cov_8.811981"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    342..435
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1429..1495
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1822 a         1840 c         1398 g         2179 t
ORIGIN      
        1 gattactgac aggatcaaaa cccaaagaac cagtctgaat ttataatctt tcaatttgta
       61 ttgtcttaac atctttaacc ccttgcttta cttctctttt gatatccatg cggatactga
      121 catccgcatt ttcaatattc aggtactttt cgatcacttt tcttatgtca cgtttcagat
      181 attttaccgt atcttccggg cagtccaggc gatccatata aagaatggaa gccatcctct
      241 gtctggcaag tacagcagat ctctgtggaa aacagaaaag aaatcgtttc aatctaactt
      301 actcccttct tcctcttttc agcagctcgc caagatgtct cnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnngttca aaatccataa gtggaaccgc ttcaccatat atccggcgac
      481 aaatattgga aaatgcctgt ccggccagac tttccgatgc agcaagtgct tcgccctgat
      541 tagtcgcgat cactacgctg tcatcatccg gaatcgcccc gagaagattt acggaaagaa
      601 tctcgacaac atcttccaca gacatcatat caccacgctt taccatgccc atccgcagcc
      661 ggttgataat aagatcaatg cgatcgatct catttgcttc cagaagacct atgatcctgt
      721 ctgcatcacg gatagcggaa acttccggcg tagtcactac gatcgccctt tttgcacctg
      781 cgatcgcatt gaaaaatccc tgctcgatcc cagccggaca gtctaaaagg ataaaatcaa
      841 acttttcctg aagatcatcc gtaagcttct tcatttgctg cggagtcacc gcggatttat
      901 ctcttgtctg agcagccggc agaagaaaca gactgggatt gtttttatcc cggatcaacg
      961 cctgcttcag cctgcagttt ccttcgatca catcaaccag attgtaaaca atccggtttt
     1021 ccagtcccat aacaacatcc agattgcgaa ggccaatgtc tgtatcgatc agaacgactc
     1081 gtccgccgag catagacagc cctgctccga gattcgcagt caccgttgtt tttccgacac
     1141 cgccttttcc ggaagttacc acaataatct cactcattac acattcctcc ataaatcttt
     1201 tcaagtcgaa cttgtggttg tcccacagcc ttttcaacga aaatgatctg ttccggcgtt
     1261 ttggccgttt gtcctgtgta cattgtgaac tgctttgtgt cttcacagtt atttttagat
     1321 tttttctgct gacccataaa cggcatccca tgtatcttgg ctcataggtt ccacatggat
     1381 atgattttct tttatataag caattttcgg ttccggcttg tattctgtnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngctcc
     1501 cgcgtaaacg ctgccacgac aggatccgag tatgacaatg ttgctttttg acacaacttt
     1561 ggcaccagaa tgtacatctc caagaatcac cacacttgtc tctgtttcca ggacttcgcc
     1621 atttgaaaga gtccctcgat aaaacaggcc atccactttt tcctgctctt ccagtgccgc
     1681 atttaaaatc ctgcggtaat gctcctgact gacggcgtcc tcatccacca cacagaggat
     1741 atggatgccg cagtgatcta cgatcgtctc gatcacctgc cgttcttctt cccggttcag
     1801 tactcttcca cggaatgtca cagccatttt tgcatttctg aaaaaattgg cagattcctg
     1861 aaatttatcc gcaatgtcaa gaaggagttc ctgaaacggc atcttgtcat ccaggataac
     1921 ggtcaggccg tatttattac tcttaatgat cacggattta cggttcatac gctcgcctcc
     1981 cccgactgtt agtccgcaaa actgttactt acatttacat ttgctgcagt atttgtaata
     2041 atatcgcttt catctttcag tttaaagtaa taacttacaa tatcttttgc aacggatgcc
     2101 gcgttcgtgg aagaatagcc gtttgtaata cgcactgcca tggcaatttc cggtgtatca
     2161 tacggtgcat agccgataaa gagtgcatgg tttggtttgt cctgattctc ctgtgcagta
     2221 cctgtctttc cggccatatt gaaagaactc tttatgtccg taaatgcggc gttattctga
     2281 taaactccat tcataccgga atggatcaca tcccagagat ctgacgaaag atccacagtg
     2341 ctgtgcacaa ccggctccgg ctcttccagc gtattattat cgctgtctgt gatacggtca
     2401 agcagagtca gatcataaca ggttccattg tttgcgatcg ttgccgcata acgtgaaagc
     2461 tgactggtcg taaaggtatt gcctgcctgt cccattgcag ttctggcacc atccgcagtg
     2521 gaaacatgcg gtgctgtctc atctatttca atgcccgtat tggcatcaaa accatacatt
     2581 gcagcatact tggtaagtgt gctgatcgcc tgtgcgtcat tataagtgga agactgtacg
     2641 acatttccat ccgcatcggt ctccggtgta agattggtaa aatccgaggt ctttccaagc
     2701 atataaccga ctgtattgaa ataaaagtta caggattccg tgatcgcctg tgacaaagtc
     2761 agaggaccat gagatccgct cttatatctt tctgaataga tccagcagtt tacatccgga
     2821 gggatcagct ggaactttcc ggtacagttg atcgtatcat aggcacctac aacaccttcc
     2881 atcacacctg cagttgccgc cacaatttta aacgtagaac caggtgctgt cacttcctgc
     2941 gttgctttat tatagaacgg agaagatttg tcgctgttca gtttatagaa ataatcatca
     3001 tccatattgt ttgccagacg attgttatca tagcccggat atgtaacaca tgccagaaca
     3061 tcgccgttgt ccggatcggt gatcacggct gaaccggtac acggctccag tgcaagcatt
     3121 gccggtgtga tctcaagaga ataaatcttc gccatgataa aatcataagc cgaaactgct
     3181 ccgttttcca gagcactgta atcttcctca ttcatcttca gaataccctg atcaaacagt
     3241 agcagacaga tatcctttcc gctgatctga taattctgta tcatatattt atatacctgg
     3301 cggctgaatg catcactctt tgaaagatag tccgtcacat aatcggcaag tgcacctgtg
     3361 atttcactgg tgtcaaggta tgtagtatcg ctgtttactt ttgtgatatc gatccagttc
     3421 ttggagatcg cataacttaa aaactcattc agactgacgg atccgtcatt gctccatttt
     3481 gtccacatct cgtcattggt atcgatcttg tctttatcaa ggacttctgt tccttcgatc
     3541 agaacatcgt ccacaataaa gctttcatat acctgcatct cctcggaaag atcctgatat
     3601 gccgttgctg aagaggatgt catctgaccc cgtatttcag agaaaatctc ttcttcccga
     3661 ctctgaaatt cactgtagat acgtttttca ttatcgcttg catcgtcaga agccagatga
     3721 tctgcatcca gcacattatt ctcaaaaagg ctgtaataca catcatagat cgggatcagg
     3781 atatcatcct ctgatgaaat gctgtcttta tcaaatttct cggcatcaaa aatcttatta
     3841 tatacaattc cggcaatata ctgctccaga atcttatata ctgcgatctg aagattgcgg
     3901 tcaatggtaa gatggatatc atttccggcc tgcggtgcaa cttcagaatc ttctttcaat
     3961 acttttccaa ggttatccac ataaaccgtt tttgttccgt tctgtccgcg aagctctttt
     4021 tcaaaatatt tctcgagtcc ggactttccg acgatatcac tgctgctgta ctgaccgttt
     4081 ccgttttcac cattaagttc ttccagttct tctgcggaaa cctgaccggt ataaccgatc
     4141 agtggtgcca tataaagacc gtcctcataa acccggatag aatcttcttc cacatccacg
     4201 cccggatagc ggtctttgtt ttccataata acagcaaccg tatctttaga aatatctttt
     4261 gcgatcgtag tcgtcacata cttctgatag ctgttctgtg caaccgcact tcgcagtgca
     4321 gtcagcttta actgctcttc ttttgtaaaa ctggatggaa ggccgtactc ctgcagctct
     4381 tcttccgtgt aaacggaaag gtcgattccg taccattttt tcgtagaaag tgtctgcatc
     4441 atctcttctg ccgtacagtt tctctcatcg gttttcaaat cttccgtatc tgcgtatccg
     4501 aaaatatctg cccggaaacg cagaagatta aagccttccc ttgtaaactc atactggccg
     4561 tcacttccaa tgtcgatcgc atagttggta taaacctggt caccgccatc ttcgatcact
     4621 ttcatgatct gatacaggct gccgttcatc gcaagatttc tctctcttgt cgtatcatag
     4681 ctgcctacat cttcataggt gacattatat gcgagcttgt tgtatgcaac cgcatttccg
     4741 tcgcggtcat aaatattgcc ccgcgtactt ttcagtgttg tttctttttt gatctgcatg
     4801 gtaaaatccg tcagatactt ttcaccgttg atgatctgca tggtatacag acgctggatc
     4861 agtatgcctg caagcaatgc aaacaggaca accagaacga tcagtctgga ctttatcagt
     4921 ttggacagtg aatttttaac ataattaaac aaagctacct acactccttt gttctgcctt
     4981 ttcaagttta tggttcaatg ccagaagcag gcggtataca accagcgtga tcagtgcagt
     5041 atagatcact tccggtataa tgatccttct cagatagaag aacaggctga tccttccgcg
     5101 aaggaaaaac tggaaaagat aaaccaccag atcatagacc agatcgctga atgcgatcag
     5161 aagtaccggc atcttgatat catcatcata aaaaatgcga taacagaaac cgttgaagta
     5221 tccgacatag gtgaagagga gcatatgaaa tcccagcacc ggtccgtaaa aaatatccat
     5281 caggatacca aaaaatgcac ccacaaacat cccctcgcgt tttccacgca tcagtgcaaa
     5341 agaaaccgtc aggatgatcg gaagattcgg ggagacggat gcaatggcaa atgtctgcat
     5401 caatgtcgtc tgcagaacaa atgtcagtaa gatcaaaata atcattatga ctctgcgttt
     5461 cactattctc ctcctgcctg cttactgctt tctgtctgtt ttccggtttc tgtttccaga
     5521 atcatctccg tctcactctg tgcctgagac tggatatctt tctgtgcctc atcggtattt
     5581 ttcagctgtg taatgaccag cacttcgtgc agatgtctga aatcaacaac cggtgtaatg
     5641 cttcctgatt ttgtcaggtt attggaatcc atcccgatct cgctgacata accgatcaga
     5701 atccccggaa ggaagcggtt actgatttct gatgtcacca ctttgtcacc gactgtcacc
     5761 ttattatccg catccgtcag cttcacaagg ctgatcttgc cttcatcgat cagtttcaaa
     5821 ttaccggcaa tgatgcagtt gtcggaagtg gagctgatct cggcactgac gttgctgtaa
     5881 tcgtctatga ttgaacgaac agtcgcccag tttgatccta ccgaagttac aatcccgaca
     5941 agacctccgt ctgcaatgac attcatatct tcctgaatcc cgtctttcga tcctttgttg
     6001 atcgtaaata cattgaacca gtttccggaa tcttttgcga taatgcgtgc ggcgatcgtc
     6061 ggatagttgc tgtattcatc tttcagatcc agcagatctt ttaatctttc atactcatcc
     6121 tgattctgca gcagatcact gttttctgcg gtcagctcgt ccactttttt ctgcagtccg
     6181 gcattttcac ttcgcagatc tgttgtatgg aacaggtatt ctcccttgtc cgagatccat
     6241 gtgccgattg cgttcattcc actctggatc ggcgtgatca ctcctgtgac ataatgaagt
     6301 ggcccggata ctgcatctgt cgtaaaagac agaatgatca aaacgacaca taccgctgtc
     6361 agaattgtga gtaccagttt gctttttcct gaatcattca tctttttttt cattgcattt
     6421 cctcttttat atcagattgc cgacataatc cttcaaagaa aatgtcagct ttttcagttc
     6481 tttgtcattc ataatctcca caagaccatt catcgtgtta tagacaggat ctggaacatt
     6541 ataaaccgga aatgttacct tttcctgtat atagctggca atattcggtg tcagtgacac
     6601 acctcccgcc agataaatac cattctgctg tatattttca agcagctgag gcggtgtcct
     6661 ctgcagtgtc atcttcacgg catcggcaat tgcatcggcc gtatcggtta tcgccttgct
     6721 gatatcttcg gacggtatcc tggcagaact tgggactccg ctgatcgtgt ggataccgta
     6781 aacttcgctg ctctttgctt ccccgttcat aagatacccc atagcaacac ggagcttctc
     6841 tgccgtcttt ttgccgattg caagattatg ctcgcggcgt accatcgtga tgactgcctg
     6901 atccattgca tttccgccat acggatactg tcttccgatg atcacttttc catctgagat
     6961 cacagaaatc ccggttgtgg aagctccgat gttgaccacc atacttcccg tctgggcaag
     7021 aaccggcata ccgatgccga ccgcatctgc aactcctttt tccacaagag cgatccttct
     7081 tactttcagt ccagtcagta ctttataaaa agcacgcatc tcaatctccg acagctccat
     7141 cggaaccgtc accagaatat ccggcctttt tgtaaaaaca ctcgaaaaac gctttaataa
     7201 ccgtgccaga ataaagccga gattcccgcc gtctgcgatc gctccgtcaa ccatcggact
     7261 gtccgctgtt acgcagaggg gcgtcttttc gtacatttcg tatgcacgct ggccgactgc
     7321 gatcacatag gttttatccc gcaccgcgat catatttttt tcacagacca cttttttgtt
     7381 ttttttatcg gcgatcttta
//
LOCUS       DAWDWH010000093         7252 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_4762_length_7252_cov_13.872864, whole genome shotgun sequence.
ACCESSION   DAWDWH010000093 DAWDWH010000000
VERSION     DAWDWH010000093.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7252)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7252)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7252
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4762_length_7252_cov_13.872864"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2245 a         1443 c         1973 g         1591 t
ORIGIN      
        1 atccacctat gagcaagctc atgtggattt agaccttttg accctggcat catcataata
       61 ataagaaaac cggcaggata tgttcatatc cgggccggtt ttctgctgcc tgaagttttc
      121 agtacttaac ttgagatgaa aatgttctgc aaaagcaaac gaaaagagtt tttctgtata
      181 ttcttccaga gacaggaaat aatgaaaaca aacgaaacta aattcgggaa acaatggagg
      241 gatataggat gcaaagatgg ctgaaacggg caggactgat tctgtttttt ggcctgtggg
      301 cagcggcagg actgcagatc tgggcaaaga agcaggaaag cagagagatg gaggcagtgg
      361 cagcgttctc gaatattgga acagacaacc gcagcggtat cgtgcagtac tatggaaagc
      421 tgaaaaaaga gaagcaggtg cagatgctgg aacgccggga agaatacctg cggcagatcg
      481 cagcctcact tggtatttcc agtcagattc tggtctatcg tacctacgga gaggacagtc
      541 agagtgtcgt actgcaaaaa gaaggggcaa acgccgatac tgtgctgcgt cttgtcacct
      601 ggagagaaaa aggggcgatc accggacaga gcctgtatgc aaacgtgact attcccggcg
      661 gcggatttga agaggcacag actttaagaa cgaaaatgca gaacgttctg gatgaaacga
      721 tggaaagcaa acgaagcagt gtggcagtgc aagggtttta tctcggaaat ctgtcagaag
      781 agaaaaaaga agcgatcgca gaacagctgc tggaagatat gcatgccaga accgtgatca
      841 aaaaccagga tgggaatttg tatacggttt atgcctacag tccctgtttt tcggagagtg
      901 taaggcaggg gacaacccgg gtaaatctgg cactgaccat gtattacaac agcaggacgc
      961 agcagacaga aatatgtgcg gcagttccgg caatcggagt gagcggggaa agtgcttgat
     1021 tatttgcaga agattgcgta tgataaagcg tatagtatct tctgacaact gcagcatgca
     1081 ttgtcaggca aataaaaatg caggaatcat gcgtgatctt tgtgctttgc aggccgggga
     1141 ttacgaagac aggagagatt atgtatacag acggaatcat atttgatgtg gacggaacac
     1201 tctgggacag cacgccgatc gtggctgagg cctggacaca ggcagttctg gaatgtggta
     1261 tcaaagaccg catcgtaaca gcgggagaat taaaacagtt atttggaaag accatgtccg
     1321 tgatcgcagc atcccttttt ccggaggctt caaaagaagt gcaggaaatc attctggaaa
     1381 aatgctgcgt ttatgaagaa gaggcactgg aagaaaatca acaggatatc tgctatccgg
     1441 atgtccggga agtcataaaa cagcttgcaa aaacatatga tctttatatt gtcagcaact
     1501 gtcagtgcgg ctacattgaa cagttcttaa gaaagacaca gctggaggcg tacataaaag
     1561 atatcgagtg tttcggaaat accggaaaga acaaaggtga aaatatccgc cttctggtga
     1621 aaagaaatag cctgaaagca ccagtttata tcggagatac caaaggggac tgcgacgcct
     1681 ccaaagaagc aggcgtgccg tttatctttg cttcttatgg atttggaaac gttacagaat
     1741 atgcagcaaa gatcggggaa atgaaagatc tgctgatgct gttttcaggg cagtaacacc
     1801 gcatgaatcg catgtgcgtt cgactgaaag ccataacttg actatatgag ctatatatgc
     1861 taccatatgt ataacaaatg tgctgcaaag agaacaagtg ctttgcagca tttgtcttag
     1921 tcggaatata cgctccgact cgaattttac tgtatatcga aggatattcc tttggtaaga
     1981 caacaaaaga gggtggcttt atggttataa aaattgattt tcagagcgaa gaagcgttgt
     2041 atatgcagct gcggaatcag atcattctcg ggatcgcaac ttctgtgatc caggagggag
     2101 attccctgcc gtccgtccgg cagctggcgg gcgatatcgg catcaacatg cacacggtca
     2161 acaaggcata ttctctgctg cgtcaggaag gatttctgac aatagaccgg agaaagggag
     2221 ccgtgatcgc actggatgtt gataaaatgg aagcaataaa tgaaattaag aaaaatctca
     2281 gagttattct ggcaaaagca cactgcaaaa atatttcagc agacgaagtg catgagctgg
     2341 ttgatgagat tttctcagaa tacgagccgt tatgatcaaa tgatgttgag gtcctaagtt
     2401 caaatgtctt atcttgcaag caagataaga ccttttgacc ctggcatcat caagtctgta
     2461 aaaagaaaga caggaggcga taagattgcg tgagcgtttt acagaaaatg cagaaaaagt
     2521 attaaagctg gcaaaaaagg cagccaaaaa taatggaaat ggatacatag gatccgagca
     2581 tctgatgctt ggacttttag aagaaggcga aggcacggcg gcgatccttt taaaagagtc
     2641 cggtgcagac accgtcaaga ttcgcacttt agttgcaaat ctgatcacat cagagggaga
     2701 taccctgaca gaagagaccg actatacgcc aaggaccgag atcattcttg agaatgccgt
     2761 gcatgaggcg gatttcttcc atatggaaaa agtggggaca gagcatctgc ttctggcact
     2821 tttaaaagat acggaatgcg tggcaaccag actgctgcac acgatggaga tcgatatccg
     2881 caaactttac ctcagcgttc ttagtgcgat ggggattgag gagtccatgt acaaagagct
     2941 ggcacagaaa gaaaaggcaa gaagggacgg cggtgcgact ccggtgctgg atcagtacag
     3001 ccgtgatctg acagagatgg cagcagaggg cgagctggac ccgatcgtcg gccgtgacgc
     3061 agagatcgag cgtattttgc agatcttaag ccgcaggacg aagaacaatc cgtgcctgat
     3121 cggtgagccg ggtgtcggca agacggcgat cgcagaaggg ctggcacagc ggatcgtgct
     3181 cggagccgtt ccaccgatgc ttcagaacaa acgcgtggtc acactggata tggcgagcat
     3241 ggttgccggt tccaaatacc gcggcgagtt tgaggaacgc atcaaaaaag tgatccagga
     3301 agtgacggac agccgcgatg tgctgctgtt tatcgatgag ctgcacacga tcatcggggc
     3361 aggcggagca gaaggagcca tggatgcctc taatatttta aaaccgtccc tttccagagg
     3421 agagatccag ctgatcggtg ccaccacgat cgaggagtac cgaaaataca tcgaaaaaga
     3481 tccggcactg gagcgacgtt tccagccggt tatggtagaa gagccgagcg aggaggagac
     3541 gcttgaaatc ttaaaaggaa tccgtccata ctatgaagaa caccatggtg ttcggatctc
     3601 cgacgaagcc ctggatgcag cagtgaaact ttccagacgt tatgtcaacg accgtttcct
     3661 tccggataag gcgatcgatc tgatggatga agcagcatca agaaagcagc tcaaaggata
     3721 tcaggcaccg aagcagcttg aaaagcttca gcagagactg gagcagattt taaaagaaaa
     3781 agaagagagc atcgttctcg gagattttga agaggcttct aatcttggac aggaacagat
     3841 cagagtagaa gaagagctga aaaaagaaca gaaaaagatg gatcgcaaac gttccggcaa
     3901 gaaactgaca gttggcgagg aagatgtagc agatgtggta gccgtatgga cgaaaatacc
     3961 ggtcagccgc ctgacagaaa aagaatccgg tcgcctctta aagcttgaat ctacactgca
     4021 taaacgagtt gtaggtcagg atgaggccgt aactgctgtt gccaaagcca tcaaacgagg
     4081 ccgtgtcgga ctgaaagatc cgaaacgtcc aatcggttct ttcctgttcc ttggtccgac
     4141 cggtgtcggc aagaccgagc tttccaaagc actggcagag accgttttcg gaagtgaaga
     4201 cgccatgatc cgcgttgata tgtccgaata catggaaaaa cacagtgttt caaaactggt
     4261 aggttcccct cctggatatg tgggatatga agaaggtgga cagctttctg agaaagtccg
     4321 cagaaatcca tattccgtga tcctgtttga cgagattgaa aaagcacacc cggatgtatt
     4381 taacatttta cttcaggtgc tggacgacgg acacatcacc gattctcagg gcagacaggt
     4441 ggatttcaag aatacgatcc tcatcatgac ctccaatgcc ggagcacagt cgatcatttc
     4501 cccgaaaaag ctgggatttg catcggtgga cgatgaaaag cacaactatg aatatatgaa
     4561 aggcagtgtg atggaagagg tacgccggat gttcaaaccg gagttcttaa accgtatcga
     4621 tgagacgatc gtcttccatg cactgaaccg tgagaatatc accaggatcg cagagatcct
     4681 gctggagcag ttcgcaaaac gctgcaaaga gcagatggat atcacactga agatatcaag
     4741 tgctgcaaaa gaactgatcg caaaagcagg ctttgatgaa aaatacggtg caagaccgct
     4801 gaaacgtgcg atccagacga aagtagaaga tgcactggca caggagatcc tggagggcaa
     4861 ggtaaaagca ggggataaag tactgcttgg cgtttctaaa gaagaaatta aattttctgt
     4921 gaaaagtgaa aaagctctgg ataagtaagt tcgattatgg taaaataaga caaaagtcga
     4981 ggaaaaatcc cataactgtt gaacactttc acatgcgttt gatttgaata tcagcaaatc
     5041 cagtcagaat catacactga gttttcgtgc atttgacttg aaagaaagct ggtttgacag
     5101 cgggataaag cgacaaaaga gaataccagg agggcataga tatgtcagta gtaagtgaat
     5161 tgatacgcac agaagcagat ggaacgatca gtttcggtaa ttatgagctg ccggccaaat
     5221 ccaaaaaatc cgattttgag catgacggcg atctgtataa agtaaaaact ttcaaggaga
     5281 tcacaaagct tgagagaaat ggcatgtttg tttacgaatc cgtaccggga accgcagtca
     5341 gccatttgaa agcgaccgag aatggtatga gctttaccgt agagtgcccg accgatgcac
     5401 agattaccgt agagctggaa gaggagacaa gctatcaggt atttgtcgat gataaggaag
     5461 ttggcgagat gaagacaaac ctcggcggca aactggtact gagcgtagag cttgacagca
     5521 cagaaagtgc ggcagttcgc atcgaaaaaa tgtaataatc tgattctgac agcaagtgtt
     5581 ttgaattatg aagaatcatt ataaaaattg agaatcttct cagcataaga aaagggggag
     5641 cgtcgggacg gacgttccct ttttgctgtg atgacgagaa aaacaaaaca gaaatatctg
     5701 ccggaaagaa tcggcagtgt tctttaaagg agggaacatg gcaaaaaaat caacaatttt
     5761 tttctgtcag aactgcggtt atgagtctgc aaaatggctg ggacagtgtc cagcctgcaa
     5821 agagtggaat acactggttg aggagaccat cgtgaccggg aaggcgggaa aaagcagtaa
     5881 aaaaacagag agcctggcgg ccaggataca tgaggcacag ccggtgaaat tatccggaat
     5941 caagacagag agcgagatac gcacagcgac cggatttgaa gagctggatc gtgtgcttgg
     6001 cggaggcatt gttgccggat cgctggttct ggtcggcggt gatccgggta tcggaaaatc
     6061 aaccattctt ttgcaggtct gcaaaaatct cacagaaaaa ggcaaaaaag tattgtatat
     6121 atcgggtgaa gaatcgcttc agcagattaa actgcgagcg tccagaatcg gaagtttcgg
     6181 agaggaaatg ctgttgctct gtgagaccag ccttgagacg atccaggggg tgatcgagcg
     6241 tgtgcagccg gaaatcgtag tgatcgattc gatccagacg atgtatcagg aagacgtttc
     6301 ctccgcaccg ggaagtgtat cgcaggtaag agaagctact tctgtattta tgcagattgc
     6361 aaaacgtatg ggaatctcca tttttattgt cggacatgtg acaaaagaag gagttgtcgc
     6421 aggaccgaga gttctggagc atatggttga taccgtgctg tatttcgagg gtgaccgcca
     6481 tgcagcctac cgcatccttc gcggcgtcaa gaaccgattc ggctccacca acgagatcgg
     6541 tgtttttgag atgtgccaga gtggattaaa agaagtcaaa aatccatcgg aatatatgtt
     6601 aagcggcaag ccgcagggag catccggatc gatcgtttcc tgctccatgg aaggtacaag
     6661 gccgattctt gtggaagttc aggcacttgt gtgccgcagc aaccttgcga tgccaagaag
     6721 gacggcagcc ggcaccgact acaaccgagt cgggcttctg atggctgtga tcgaaaaacg
     6781 catcggtatg catttatcaa cctgtgatgc ctatgtcaac attgcgggag gcatccgcat
     6841 gaacgagccg gcgatcgacc tggcgatcgt gcttgcactg atctccagtt acaaagacat
     6901 tgcgattgac gaaaaaacac tggcattcgg agaagtgggc ttaagcggtg agatccgtgc
     6961 cgtcagcatg gcacaacaga gagtggcaga ggcaaaaaaa ctgggctttg agacgatcat
     7021 tctgccggca gtttccatgc cctcaattgg gaaagtggaa ggaatccgcc tgcttccggc
     7081 ggcaaattta agggaagcat tgaaatatct gtaaaagtta caatgggggt gaagatattt
     7141 tgtacaaaat catgctgcta gatgacgaag cggaagtccg agaaagtatt ttgcttcaga
     7201 tttcgaagag cagttgcatc agcaggaaat gtagcatctg caacgcagag ta
//
LOCUS       DAWDWH010000094         7196 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_4809_length_7196_cov_10.937124, whole genome shotgun sequence.
ACCESSION   DAWDWH010000094 DAWDWH010000000
VERSION     DAWDWH010000094.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7196)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7196)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7196
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4809_length_7196_cov_10.937124"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3130..3139
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1819 a         1754 c         1588 g         2025 t
ORIGIN      
        1 atccgatgat cgtcagcatg caggcaataa atgtattacc gacagagatt ctggcgatcg
       61 catagaatgc cagcacgatc agtacgtcgt ggagcagagc cagaacggca cttgcaccga
      121 aacggatatc tttgaaacgc agccagatgt agatcagcat gcagatagct gcaatgataa
      181 ccgcaacgat tgcatcctga cgcatttcat tacttacggt agaagaaatg ctttctgcgg
      241 tgattgtgga ttcatccaca ccaaattttt cattcagttt ctggctaagc tcggttctct
      301 catccagaga cagttctctt gtcttgatga taacctgatt ggaaccgctt accttggtag
      361 cctggatatt tgcatcgccg gttacttcct gtacaaccgg ttttactttt tcatcgatct
      421 cttcgatgga gtaatcttcg ttgaaggtta cgtttgtaga agtaccgccc ataaactcaa
      481 ggctgtagtt cagtgctttt ccactgccct ggctgtggat cagcatggca accggaccgg
      541 agatcatgca tacgatcgcg attgcaaaga ataccttctt atgtttcagg aaatcaatgg
      601 tgttatgttt tttcgggcat ccataatatt tcacatcctg gaatccgaga gcataaaatg
      661 cctttgccag atatctggag ataaccagtg cggagaacat ggacaggatg atactcattg
      721 ccagtgtgta tgcgaaacct tttacagaac cggttcccat gatgcccagc acgatcgctg
      781 cgatcagggt tgtgatattt ccgtccagga ttgcagacag agcttttttg aaaccgatat
      841 cgatcgcact tttcacggat ttaccggctg cgatttcttc acggatacgt gcatagatga
      901 taacgtttgc atcgaccgcc ataccgatac caaggatgat acctgcgata cccggcagag
      961 tcagtgtgat atcaaatgca tttaaagtaa ccagtgtcag tacggtgtac agcagcagag
     1021 agatcgctgc aacaacacct ggcagcaggt aaacaaccat cataaagatg cagacaagtg
     1081 ccagaccgat cgctcctgct tttaagctgg tggaaattgc ggcttcccca agctgagcac
     1141 ctacaacgtt ggaacgcagc tcctgcagtt ctagggacag agaaccgata cggatggaag
     1201 aagccagatt ttctgcttcc tgtgcatctg ccataccggt gatgacagca gaaccgtctg
     1261 tgatcgcact ctttacggtc ggagcactga tgacatctcc atcgtataca atatagatgg
     1321 atttgccgat attttctgaa gttgcagttg caaaggctgc ggcacctgca ctgtcaaact
     1381 ccagttttac aacgttctca ttgtttttca ggttatcctg ctgaacgcct gcttctgcgg
     1441 atgcgacctg gcttccgtct acgacaacag tgccgtcttc cagctggaac tgaagggaac
     1501 ctggtttgcc aagttcttca agaatggtat ttgcatcgga tacaccaggg atttcgatgt
     1561 tgatacggtt agtaccttcc tggtaaacct gtgcttctgt gctgtagccg tcaacacgct
     1621 gctgcagctt gtaaatcgtg tctttcatat cttcggcaga cggattttca tcacccacta
     1681 cctcatacgt gatacttaca ccgccggaca ggtccagacc aagtgtgatg tttttggcag
     1741 ctcccgttcc ggtaggtccg aagccgacag ctgtggtgta ggtcaaaagc ccaagaaaca
     1801 gggcaatcgc aacgactaca ccaatcgctt tgttcttttt catgtttctt aattctcctt
     1861 ttcctcggca gcgagtgctg cctttatcat gatcgcgcat tcgatacgct tccgctgcaa
     1921 ttcacatccg atatcatatg catcgttgat ggatttgtgg aaattccagg aattcgccgc
     1981 acatccgccg ctgcagtaga attttgcaaa acagtttttg cacttttctt ttgcatagac
     2041 attgcagttt ttaaactcag cctggatctg tggtgttttg ataccttcct ccacatttcc
     2101 catcaggaac tgttcttccc cgacgaactg atgacacgga taaaaatcac cccacggtgt
     2161 gacagcgagg tattctgttc cggagccgca tccggagaga cgtttgtaaa cgcatggacc
     2221 tcccgtcagg tctatcataa aatggaagaa attgaatccc cttccttcct tatgacgctt
     2281 gatcatttcc tttgccagtc tgtcatattc ttcgcagatt gccggaatat cttcttcccg
     2341 cagtgcgtac tcctcttccg gctgagcaac aaccggctcc acggagatct gtttgaagcc
     2401 cagatctgcc agatgcagga catcggatgc aaaatccaag ttgtgtctgg taaaggtccc
     2461 tcttacataa tatttatcct gatgtctgct ctccgcaaat ttttcaaatt tcgggagaat
     2521 caggtcatag cttccttttc ctttgcggaa cggacgcatg tagtcgtgta cttcttttct
     2581 gccgtcgatg ctgagtacga catttgccat ctcacggtta cagaactcca taacctcatc
     2641 gtttaacaga acgccgtttg tagtcagtgt aaaacggaaa tttttgttat ggatcttctc
     2701 ctgctctctt ccgtatgcaa ccaggtcttt tactacctgc cagttcatca gcggctcccc
     2761 gccaaagaaa tccacttcca gatttcttct gcttccggaa tttgcgatca ggaaatccag
     2821 tgcttttttg ccgacttcgt agctcatcag ggcacgacgg ccatgatact caccttcttc
     2881 tgcaaagcag taacgacagg cgaggttaca gtcgtgagca atgtgcaggc agagtgcctt
     2941 cacaacagtc ggacggtttt taaatgcgtc gattgcattt tcatagatat cttcggtaaa
     3001 cagtgccccg gcttcttcca gttcatgaca ttcttccagt gcttcttcga tctctgccgg
     3061 agcatagctg ttcgcaagtg cttttaagat ctcttctttt gattcttttt tttcgaggta
     3121 tggcagtacn nnnnnnnnna catcgtacac cacatcatct acgatatgta cggaaccgct
     3181 gtttacatcc ataacgatgt taaagccgtt gcttttatat tgatggatca acccgtaatc
     3241 ccctttcagt tctccagacg ctaagaaaca gcgagcaaga tgcccgctgc ttgcttgtat
     3301 gtcctgttat tatttattgc tttcgcagct ctgatttcct acggtgcagg atgtcttaca
     3361 tgcggactgg caagatgtct gacattcgcc gcatccgcct ttctttactg tgttctgcag
     3421 acttttggta tttaatgtct gaatatgttt catattatcg cctccttatc ttcgcttacg
     3481 cggaaattta attagattat aacataatgt tcacaatagt aaaagcattt tttttacaat
     3541 aacacaactt tttcacaaat ccatgttata ataagacaca atcataggat cggcaactgc
     3601 gatccgcatc cattttaata cagtcgaaag gaataattta actatgaaaa agaaagttgc
     3661 aacagctgcc attgcagcaa tgaccatgac atttctggca gggtgttctt ccaggggcac
     3721 ttatgccaaa tacctgacac ttggcgaata caaaggtctg agcgtcacca aagtaaaaac
     3781 ggaagtaacc gatgacgatg tggaggctga gattgaaagt gccctggatg aaaatgcgga
     3841 gtatacggat gtagaccgtg ccgcaaaaga cggcgatcag gtaaacattg acttcgacgg
     3901 aagcattgac ggagaagcat gggattcgga taccgactac gatctggtga tcggttcgga
     3961 ggatttccag ccagagttcg aagaacatct gatcggtgca aaagccggcg atacactgga
     4021 atttacaatc acgctctcgg atgattacga tggtgattac gccggaaaag atgcagattt
     4081 caaagtaacg gtcaatgcgg tgcaggagat caacgttccg gaactgaatg acgattactg
     4141 caagaagtac accgactatg ataccgttga tgaataccgt gccggtgtca gaaaagagct
     4201 ggaagaatat tatgatgaga caaacaccga gacggcccag aatgatctgc tctcccagat
     4261 ccttgaaaac tccaaagttt ccggttatcc gcaggatctc tacgataaat gcaaagaaga
     4321 atatgatgcc aacaaccaga ttcttgcaga catgtttggt accgatgtct ctgacattgc
     4381 aggctccgaa gatgacgtca agagtgccgt agaagaaatg gtttatacgg aaatgctgac
     4441 gaccgcgatc gcagaaaagg aaaatatcgc cgtttcggat gatgaataca ccgactacgt
     4501 caactccgtc tatgaggaga gcggctacac ttccgcagaa gaatatgaag aagattacac
     4561 caaagacgag acagtaaata ccatcctcta taataaagtc atggattttc tgatgcagaa
     4621 tgcaaccgtt acagaagttt ctgaggatga gtactatgaa gaagataccg agatggatga
     4681 ggaagcgtac gataccgaag aaatcacgga aaatgagaca gatgccgaag atttggagat
     4741 gacagagtaa tgtcccaaaa aaagaaaagt atgttgttat ttctggtagt tgccctcctt
     4801 cttgtcctgt cgcttgtggc tgtctttttt ctgaacagcc acaacgaaca gggcgagatc
     4861 gcccgtattt atcaggacgg taagctgatc cgcgaagtgt cactgaccga tgttgccagt
     4921 ccttatgaat ttcaggtgga ggcttcggat ggcggctaca acctgatccg ggtagaaaaa
     4981 ggggcaatcg gcatcaaaga tgccgactgc ccggacaaaa tctgccagca gagaggcatg
     5041 gtgtcggata ccggatatcc ggtcacctgc ctgccccaca agctggttat tcaaattgaa
     5101 cgccttactg ccgcctccga tggtatcgat gcggtcgtgc agtaagaatc ctactcttct
     5161 cccgctcttg cagcgatctc cttatccatg atatctttcg gtgcctgctc atagcgtgca
     5221 aactcatatg cgaaatcgcc gcttccacct gtcatggaac gtaacgttgt gcagtagccg
     5281 taaagctcca tcatcgggat gtccgcttct acgatctgat tgcctttatg atctggattc
     5341 atgccaagca cacggccacg gcgtttgttc agatcaccca tcacgtctcc ggtaaattta
     5401 tccggaacgg tgactttcaa agatgcgatc ggctccagca gtattggtga tgcctcaagg
     5461 aatccttttt taaatgcctg aatggatgcg gtcttgaatg ccatttcaga ggagtcaacc
     5521 ggatgatagg agccatcata aagaatggct tttacaccga caaccgggta acctgccaga
     5581 ggtcccttta atacagcttc ctggacacct ttttctacag ccgggaagta gttcttcgga
     5641 acggctccgc cgaccacttc ctgttcgaat gcataaggtg tttccagatc tcccagcggc
     5701 tcaaaacgca tcttgacgtg accatactgt ccatgtccgc cggactgctt tttgtattta
     5761 gcttccacat cggcactctt gcggatcgtc tcacggaatg caacctttgg tctttccagt
     5821 tcaatctcga tcttgtactt cgtgagcaat tcgctctgaa tcatctcaag atgctggtcg
     5881 cccataccat agagcagtgt ctgacggttc tcagaatcat tgatcacttt cattgtcaga
     5941 tcttcgtggg aaatcttctg aagtgcctga gctactttat cctcgtcccc tttgtttttt
     6001 gcacggtagc gtttgtaagt atatggaatg gaaacttcga tcttgccgta acggatcggt
     6061 gttgccttag tagaaagggt atcgccggta cgtgccttgt cgagtttggc gatcgccccg
     6121 atgtctccgg catgaagctc tggaatctcg atcggtttgc ttccttcaaa tacatacagc
     6181 ttattcagct tctgttctgc atctttgtca atattgaaaa tgacatcatc actcttgata
     6241 acgccggatt tcactttgat catagaatat tttccgatga acggatcgac gatcgtctta
     6301 aatacctgtg cggattttgc ttttgcaaaa tcataatttg cttcaaaaat ctcgtttgtc
     6361 tttgtattga tgccggctac actgcgtttg tccggacttg ggaaatactg aacaatgtca
     6421 tctaaaagat ttgccacgcc gcggagctga accggtgagc ccatcgctac cggaacgata
     6481 tcgccgctgg aaacgttctg tgtcagtgcc atcatgatct catgcacgga gaactcttct
     6541 cctgcaaaat agcgatccat aaactcttcg cttgtctctg caactgcttc cagcagagtt
     6601 tctctatatt tttcaagata ttcatccaga tattcaggaa tcggacattc ttctttctgg
     6661 cctttttcca gccattttct tcctgctttc tttactacgt tgacatatcc tgtaaatttc
     6721 tcattctcac ggatcgggaa gtggatcggt gcgatcttct ttccgaactg ttccgtcaga
     6781 tcttcggtga tctttcggaa acttgcattg tcaaaatcca tatttgtgat aaaaatcatg
     6841 cgtgggagac tgtatttctc acacagccca tgccttcaaa gtaccaactt ctacgccgtt
     6901 ctttccgttt acaacgatca cggctgcatc tgctgccgct gctgcttctt ctgcttctcc
     6961 tacaaaatca aaataaccag gtgcatccag aatgttcacc tttacatcgc cccaggcgat
     7021 cggtactaaa gaagtgctga tcgagaattt tctcttgatc tcttctttgt caaagtcact
     7081 gatagtatta ccgtccgtca catttcccag tctgcttgtg ataccgccaa gatacgccat
     7141 ggcttcaacc agactggttt tgcccgagcc tccatgccca aggagcacga catttc
//
LOCUS       DAWDWH010000095         7158 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_4836_length_7158_cov_12.594960, whole genome shotgun sequence.
ACCESSION   DAWDWH010000095 DAWDWH010000000
VERSION     DAWDWH010000095.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7158)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4836_length_7158_cov_12.594960"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2064..2073
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2190 a         1370 c         1853 g         1735 t
ORIGIN      
        1 agtgaaatcg accgtctggt aaatattgta aaagaaaatc agtgtgatct tgtgatcggt
       61 atcggcggag gcaagatctt tgatacagcg aaagcagttg catattatgc aggcacaccg
      121 gtatttatct gcccgacgat cgcatcaact gatgcaccgt gcagtgcact gtccgttgtt
      181 tatacagaag aaggtatctt tgaaaaatat ctgttccttc cggccaatcc gaatctggtt
      241 ctgatggaca ccgatattat cacaaaatca ccggtacgcc ttacagtagc cggtatggga
      301 gatgcacttg caacttactt cgaggcgaga gcatgcaaac gcagcggtgc aacttcctgt
      361 gcaggcggaa agacaacgga agcagcgatg gcacttgcaa aactttgctt tgatacgctg
      421 atggaagaag gcgtaaaagc caaaattgca ctggaagcag gtgtatgtac accggcagtc
      481 gaaaaagtga tcgaagcgaa cacgctcctt tccggtatcg gatttgaaag tgccggactt
      541 gcgggagcac atgcgatcca caatggattt acagtactgg aagaatgcca ccatatgtat
      601 catggagaaa aagttgcatt tggaacactt actcagctgg tactggaaaa cgttccggaa
      661 gaagaactgg aagatatcat tttgtggtgc atcgaagtcg gactgcctgt taccctggca
      721 gaacttggtg ccggcaatgt gacagatgag cagctgatgg aagttgcaaa gaccgcctgt
      781 gcggaaaatg atacacttca caatatgcct tttgaagtga caccggaaac tgtatttgca
      841 gcaattaaag cagcagatgc ttatggaaaa tattatctgg gtgaagaata agatataaga
      901 tcatcacata gttgaatatg atattataag aagaccggca ggatatggaa aatgttccta
      961 ccggtcttct tgcaaaattt tctgttttct gtggaacgaa taaagatatc aggtattgca
     1021 aaaagaacaa atgttttcag tacactgttt cacaaagata tttaacaaaa aagctcaagg
     1081 aggtacgaaa gagatgaaaa ggaaatggat cgcattagta ctcagtgtgt cggtactggc
     1141 aggaacggca gctgttcctg catttgcttc ggagatgcag caggagatct cggagatgcc
     1201 ggcagtggaa acacttcagg atcatacgct tgcagagacg gattccgtgg aggagaactg
     1261 tgttctggtt ggactgaaag gcagctatct ggcaagtgca gatgcggcat tgaaacggat
     1321 caatgagatc cgaaaagagg cgtgcaaaca gggcgttcag gatccgcgtg atcccaaccg
     1381 aaagcttacg atgtcagatt atgtaccggt caaatggtct tctgatctgg aatatatcgc
     1441 aagagtccgg gcagcagaag catctgtgta tatggatcat caaagaccaa atggaacgat
     1501 gtgtttttca caggcatcac caaatggggt gcagagctgg ggagaagttc tggcatggaa
     1561 taacagcaat gatatgatca ccggaatcga tcagtggtac ggtgaaaagc aggactgggt
     1621 aaaacagacc ggtggggtga caggccatta tacatcgatg atcaatccga acaatcttta
     1681 tgttggactt gcaaccttca tttgcccgga tgcagatttc aaaaatacga catccgggga
     1741 gttcagtttt gaaacaggac tggatgaagg ccaggcaaaa gcagtaaaga ataaagtgca
     1801 gaaaatccag gttcagaacc aggatgtaaa ggcatacatg gagcctttta aagagaaact
     1861 ggcctcttcc aaaacggtgc aggcgaaatt ttatgcaaat tacagaggct cggggttcta
     1921 tcagtcacgc acacacaaac tctcttttga ggacacagta gagtggagtt cttccaatcc
     1981 gaaagtggca gctgtagatg aaaaaggtgt agttaccggt gtgagtgcag gaacagccaa
     2041 aattacagca aaatgcggta tttnnnnnnn nnntaaaaaa tgcccgctgt tgcgggcaaa
     2101 aaataatcag agcttacacg ccgataaatg ctctcagttt ttctatatca tttgttaaga
     2161 tcaaagactc atcaaactgc agttcttcca gaagtttgac ggcgagatca aaacgtccta
     2221 cccagctcgg atcatgggca tcggagctta taataatcgg aacttgctgc tgctcacaga
     2281 gtttcagcat cttccgataa ttttcatagc aattcagacg gcggtctttt ttgaccagcg
     2341 aactgttgtt gacttccaga gcgacccggt actgtttggc agctgaaacg agacgttcat
     2401 agtcaagtgg tgtatgatca tcatccgggt gagagacaaa atgtactttc tcatttttca
     2461 tacatgcgat caagttgtcg gtgttctttt cttttccttc atcagtataa cactctttgt
     2521 ggattccgac gattgcataa tccagaaaat caatatattt ctgttccaga gacagttttc
     2581 cgccatttaa aacattgatc tcacatccgt gcaggatctg tacattcgaa agcacgcgag
     2641 ggatgactgt gagattcaga taataaaaag gatcgaccgt tcccggaatg ccgggtgcgt
     2701 gttctgtgat cccgatcagt ttcagatttt tttcacctgc agtggcagcc atttcgcgta
     2761 tcgttccgta tgcatgtccg ctgacaaggg tgtgcatgtg catatctgca attagttttt
     2821 tcataaagaa agactccctt ctgtacagag attgtttcag atttttgatt tcagaatttt
     2881 gcttcctgaa aaggatgcct gcgaagtact ttgcacaatt ccgacatctg atattataac
     2941 gcttaccatg tgaaaatcaa gaataacgca gtttccggtt gaaagaaaac gatttataag
     3001 atagaatgta actattcgtc ttattataac acttccccaa aggaggagca acattggaaa
     3061 acacagaaaa ttatctggaa tgtgaaagaa accgagtgta ttataccctg attgcaatcg
     3121 caggattttt tggtgcgtat acctttttgt tacgcgggaa agtattctgt aatgctcaga
     3181 cagggaatct ggtgctgctt ggactggcga tcggtcaggc agaatgggaa acggcagcct
     3241 actacctgtt cccgatttcc gcgtatatgg caggtgcatt tatctcagaa ctgctgccga
     3301 acccagtcaa gtatcatctg aaaatccgct gggacaccct tctgattgca gttgaaatgg
     3361 caaccgttct ggtgcttgga cttgtaccgg aatccgcacc ggtgcagatc tcacaggttg
     3421 cggtcaactt tattgcttcc atgcagtaca atacgtttcg tcaggcagag ggaacgccga
     3481 tggcgacaac ttttgcgaca aaccatatcc gccaggtcgg tgtgggactg gcaaaagaaa
     3541 tccagcattt tcggaaaaaa gataaaaaac atcgagaaaa atggggcaaa cattgcatga
     3601 tggtggtata tttcttatta ggtgtggtgt ttggaacggt tgcctgcaat atttattccg
     3661 ggaaggcaat ctggatcact ctgctgccgt ttctgatcgt tctgattgat ttcctgaatg
     3721 aggatctgca tgcagaagaa gagaaaatga gacagaaacc acatggacat taagaaaaag
     3781 gcgtaagaca ggttcaggtt gaacgcacgt gaggcagagg cattatgaat atcaatgaaa
     3841 ttgcagaact ggccggtgtt tccagggcga cggtatcccg ctatctgaat gatggatatg
     3901 taagcgaaga aaaaagggca gcaatccgga aagtgataga agagaccggc tatcgcccgt
     3961 cacagcaggc gagaaattta agaagcaaag tgacaaagct ggtcggtgtg atcattccgg
     4021 agattgagtc cgggtcggtg agccagatgg tgtcagggat cagtgaggtt ctttcggtaa
     4081 aaggttatca gcttttgctg gcagatatgc ggaataacac cagggcggag ctgaaatacc
     4141 tccgtatttt cgaaaaaaat caggttgacg gcatcatttt catgggaacg atgctttcca
     4201 gagaacatct gcacctgatg gaagaagtcg aagtgccgat cgtggtgacg gcacaggaga
     4261 tccggcaata ttcctgcgtt tttcaagatg attatcatgc ggcatatgat gctgcaaaaa
     4321 tgctgacgaa aaaaggcaga cacatcgcct atatcggaga gccggcaaaa gacaaagcgg
     4381 caggaaaagc acgaaagagc ggatttctgg atgcaatggc agatgccggc atcaggatgg
     4441 acagatcctg tatgtatgaa acggaattgt gcatgcaggg cggttacgag aaagctgcaa
     4501 aaatatggaa aacacatccg gaagtggact ctctgttctg tgcaacggac agcctcgccg
     4561 tcggagcact gcggtatttt catgagcatg atatccaggt tccgcagcag gttcagatcg
     4621 tggcgtttgg agacaccgag ataggaagtg cggtgtcacc ggcgatcagc agtgtgcatt
     4681 tctaccacaa aacgatgggg caggaagcag ccaggatgct ggttgaaatt ttagaatccg
     4741 gcgatgatct caaaaaacat ctgaaaatgg gttatcagat tgtgaaaaaa gaatcgcttc
     4801 ggtaaaaaaa gaacgcagca gacaaaaaca gtcgcagcgt tcttttttga tttgcagaag
     4861 gcaaaataca gtataataaa cgaaaaagga aagcgaggaa tctcatgcag gaacgatata
     4921 tttcatcagg cagcagcaga ctgcacattg cagtttggga accatcagga gagagaaaag
     4981 caatcatgca gatttcgcat ggcatgatcg aatacatcaa aaggtacagt cattttgcga
     5041 attacctcaa tcaggcagga atcctggtga tcgggaatga ccacaaggga cacgggctga
     5101 cagcagaaaa ggatgaggat ctcggatatt tcggtgaaga aaaaagtgcg gcggtgatcg
     5161 aagatcttca cgaagtcaca cgttatgcga aggaacagta tggagcggat gtgccgtatt
     5221 ttctgtttgg gcacagcatg ggatcgttta tggcgagacg ctatttgatg acctatggcg
     5281 aggaactgac cggggcaatt atctgcggga ccggttacac gccgggggca gttttgacgg
     5341 ccggaaaact ctgtgcgggt gtgatcagta aagtaaaagg agaacgtcac cgctcgaagt
     5401 ttttgcagaa gctggccttt ggcagttaca acaaacgcat cgcagataag cggacagcaa
     5461 acgactggct gacaaaaaat acggaaatgg tggatgccta taaccgggat aaatactgca
     5521 catttctgtt tacggtcaat ggctatcaga cgctgtttga tgtccttgga tttatccaga
     5581 agaaggaaaa tatcgagaaa ataccgaaaa atctgccagt ctatatgatc gccggcgagg
     5641 aagatccggt gggcaattac ggaaaaggtg tggaaaaagt atttgaagaa taccgaaaat
     5701 gtggaatccg tgatctggaa ctgaagttat atgaggatga ccggcacgag atactcaatg
     5761 aactggacag agagaatgtt tacctggatg taaaaaactg gattttgaaa catatttaaa
     5821 tatcagatgt gagatgatgc ctgcttttgc aggcggcgag caacaagctt gtatcagtgg
     5881 cggattcaat cgccaagtgg aataatagaa agcgagaaat gatggaacgc ataaaaccga
     5941 attattatga tagattcacc tgcattgcag acaagtgtac tttcacctgc tgtcaggagt
     6001 ggaagatcgc agtggacaac gagaccaacc gaaaatggaa aaagaaatat ccgcctcagg
     6061 aagtgaaggt gcagcggaaa aatctcagtg cctatacaac caaaaagaac ggaggcagag
     6121 tgatccgact ggatgaagag cataaatgtc cgtttctttc tgaaaatcga ctctgcagac
     6181 tggtatcggc ttatggggat tcggttctgt cggaaacctg taccgttttc ccaagagaag
     6241 tgcacagatt tgaaacgcat gaagaagaag ttctgatgcc atgctgcccg gcggtgatcg
     6301 atctctgggc agaggaagag aacgtggttt ttccggaggt gccggaaaac ggcagaaatc
     6361 tgcggacgat gatccgtgaa aagatgatgg aaatgttccg aaacaaagaa cagagcatcg
     6421 aagaaacgct gctagagagt ttttatattc tgctggaatt atatcaggca gagcaggaag
     6481 atacgctgtc agaagaaaga atcgagaact atttctccag agaagccgtg aaagaactga
     6541 aaacggcgat tcttgcgatt gatctgccgt ttctggatac catggatgaa tgcaatgagc
     6601 ttttgcagga tctggctgtc aattatcaga aagaaggtct gtatcaggat tatctgacga
     6661 agatcctgcc tctggcagag cagctttccg aaaggtacga tgccgatgaa atggagcaga
     6721 aaggacagga ctttgcaaag caatgggaaa aataccgaaa actcctggct gtttttatgg
     6781 aaaatgaact gttttcggat cttctgatgc cggacggtga tttggagagc atggtcattc
     6841 agatgcagtg gatcgcaatg gaatatacta tgatccggca cagtctcttc ctgaaatgga
     6901 tgcaggacgg atgccgggat cttgcatacg aaacactgcg ggattatctg gtgctgatca
     6961 cacggatgac aggatacgaa gaagacgata tttatgaata tctggaaaac agttttgatg
     7021 aactggtatg ggagtgggga tatttatcgt taattgtcgg caaataaggt tgatttcttt
     7081 atcaggaaga tttataattt aaaacacata aaaagtacag aaatatcgat gaatttttgt
     7141 gcagaaatac aaagcaac
//
LOCUS       DAWDWH010000096         7047 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_4917_length_7047_cov_9.296911, whole genome shotgun sequence.
ACCESSION   DAWDWH010000096 DAWDWH010000000
VERSION     DAWDWH010000096.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7047)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7047
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4917_length_7047_cov_9.296911"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    943..952
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2380..2389
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3004..3013
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3713..3722
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2360 a         1332 c         1759 g         1556 t
ORIGIN      
        1 ttgaatgcaa tccggggaca ctgacggaag aaaaactgca gatttaccaa aaagcaggga
       61 tcaatcgttt aagcctgggg ctgcagtccg cagatcagga ggagttaaag cttttaggac
      121 gtatccacac gtacaatcag ttcaaagaga atttttatct ggcaagaagg atcggatttg
      181 cgaatatcaa tgtcgatctg atgtcggcac ttccgggtca gagtgtagag agctatcgct
      241 gtactctgaa aaaggtatgt gaacttggac cggagcatat ctccgcctac agtctgatca
      301 tcgaggatgg cacgccgttt gcaaagcgtt acagacagga tgccgcaaaa agagaagccg
      361 gagaaaaacc gcagtttttg ccttcggagg atgaggaacg tcagatgtac gaggatacgg
      421 cagagattct ttccgaatat ggatatcatc gttatgaaat atcgaattat gcaaaagcag
      481 ggtatgtatg cagacacaac gtcggatact ggaaacgagt ggactacatc ggttttggac
      541 ttggagcatc cacattgcag aatccgctcc gttataaaaa taccgatgat ctgcaggaat
      601 acttaaaccg ggatttctca aaaaaggaat tactggtgct gacaaaagac aatcagattg
      661 aagaaacctt gtttttggga cttcgcatga tggaaggcgt cagcctgtct gcatttgaag
      721 agcattttca ctgtccgctg acggtgatct accaaaggga gatccaaaag ctcacagaaa
      781 aaggattgct gagaattgaa gaggaccgca tttccttgac agaaaaaggg attgatttaa
      841 gcaacatggt gctttctgag tttttgctcg gataaagata aaacagaaaa gcggattgca
      901 aatgtttgct ttatacagaa atggcagaac aagctgaaat gtnnnnnnnn nncattctgt
      961 tccagataat catagcaatc caggtactga tccggaaaag attcttttaa ttttgcatta
     1021 aaatcccgga tttcttcgtt ggaagcaccg gagcatgcat cgcctaccgg attcacagac
     1081 ataatataga aagaagcgtt cggatattct gcaaaaattt gaggataaaa cgcaagataa
     1141 cggtcgatct cactcagatc attgacgcca agattaaaca caacagtagc atccggctct
     1201 ttggaaagag aggtgcgaag ctgatcgatg ccctcgttca caagccagta gtaaccttcc
     1261 ccgacttttc ctatataaga gcaggtatcc gcatcatcgg aatcctgcat ggcttcctcc
     1321 atgccgaccg ttctggaatc tccgacaaaa agaaagtgat gacaggaaga ggagacaata
     1381 tggtcagaag tgtctgcggt atcagatgaa tcctgcacag aatgttcgtt tttcacatca
     1441 cgtaagcttt tttccagaaa agcggtctgt tcctttgtaa accaggtcat aaatacggcc
     1501 agtgcgatca gagctgcaat cagaaaactg agcagtacaa tgacaagatt tgtggttttt
     1561 cgtttcataa gaaccccctg aaattttatg tgatcgtttt tttaggtcag attctgtttt
     1621 tctgacctct ttagtataac gcattccgga aaaaagtcaa tttttataaa aaatgaaaaa
     1681 aacctcttga caaagaatat gcgtagtgct attttaggta caaggatgtt agcactccac
     1741 taagatgagt gctaataaca ggaaggagga ttcaaggatg cagttagatg agagaaaatg
     1801 gaagatactt caggcgatca tcaagacgta tctggagacg ggagagccgg ttggttcgag
     1861 gacgatctcc aaatatgcgg atttgaatct gagctccgca accatccgaa atgaaatggc
     1921 agatctggaa gagcttggat acatcatcca gccgcatact tcagccggca ggattccttc
     1981 agaccttggt tatcgtctgt atgtgaacca cctgatggaa gagaaagaac agcaggtaac
     2041 ggaaatgaag gagctgatga tcgaaaagca ggataagatg gaaatggtcc tgaagcgtgt
     2101 ggcacagttc cttgcagcca acacaaatta tgcttcgatg atcacagggc cgcagtatca
     2161 cagaacaaag cttaagttta tccagctttc catagtaagc gaagatcagc tgttagctgt
     2221 tgttgttacg gaaggcaacg tagtgaagaa taaaatgatc tcgcttcatc acggactgga
     2281 tcaggaaacg gtattgaagc tgaacattct gcttaacacc agtttgaatg gtcttacgat
     2341 ggatcagatc aatctgacaa cgatcgcaaa gatcaaagan nnnnnnnnng cgttctggat
     2401 gcagtcgcag aagcgatcca gatggatgaa gaagagatgg agatctatac aagcggagcg
     2461 acgaacatct tcaaatatcc ggaactgtca gacagtgaaa aggcgagcag cctgatcggt
     2521 acctttgaag aaaagaaaca gctggcggat ctggtagccg atacgatggc agatgacaag
     2581 aacaccggca ttcaggttta catcggaaat gagacaccgg tgcagacgat gaaggactgc
     2641 agtgttgtga cagctactta cgagctgggc gatggtatgc gaggtacaat cggtatcatc
     2701 ggaccgaaaa gaatggatta tgacagagtt gtcggtacct taaaaacact gacaactcaa
     2761 ttggatgata tatttggtaa gaaaaaagaa gacaactaga gaggtgattg tatggcagag
     2821 gaaacaaaaa acacaaagcc tgctgatgaa gaaatagaaa aaactgcaga accgactgca
     2881 ggagaagaaa aacagccgga aacggaaaag gcagaaagcg atacagctgc cgcaaaagaa
     2941 gaacagccgg aaaagaaagg cttctttggt aagaaaaaag aaaagaaaga taaaaaagac
     3001 gagnnnnnnn nnngactgat cgtctgaaac gccagatggc agagtttgat aatttccgta
     3061 aaagaacgga aaaagaaaaa gcagccatgt ttgaggtcgg tgcaaagagt gtcattgaaa
     3121 agatgcttcc ggtgatcgat aatttcgaac gcggactggc aactctttcc gatgaggaaa
     3181 aagaacagcc atttgcacag ggtatggata agatctacaa acagtttgta gcaagcctgg
     3241 aagagctcgg agtaaaagcc atcgaggcag aaggaaatga gttcgatccg aatttccata
     3301 atgcagtgat gcatgtggaa gatgaacagt acggcgaaaa tgtcgtagcg gaagaacttc
     3361 agaaaggcta tatgtaccgt gacagtgtgg ttcgccacag catggtaaaa gtagcaaact
     3421 aaattaaaaa gttgacaaaa ttcagtataa aagcagaata aaacaggagg aatttatcat
     3481 gggtaaaatt attggtattg acttaggaac aacgaacagc tgtgtagcag ttatggaagg
     3541 tggcaaaccg gttgttatcg caaatacaga aggtgcaaga acaacaccat ctgttgtggc
     3601 attcacaaaa acaggtgaga gactggtcgg tgaacctgca aaacgtcagg cagttacaaa
     3661 cgctgacaaa acaatttctt ctattaaaag acatatgggt accgattatc gtnnnnnnnn
     3721 nnatcgtgta accatcgacg acaagaaata ttctccgcag gagatttccg caatgatcct
     3781 gcagaaactg aaagcagatg cagaaaacta cctgggcgaa aaagttaccg aagcagttat
     3841 cacggtaccg gcatatttta atgatgcaca gcgtcaggca accaaagatg ccggtaagat
     3901 cgcaggtctg gatgtaaaac gtatcatcaa cgagccgacc gcagcagctc tggcatatgg
     3961 actggacaat gaaaaagaac agaagatcat ggtatacgac ttaggtggcg gtacattcga
     4021 cgtatccatc attgagatcg gtgacggcgt tatcgaagta ctgtcaacag ccggagacaa
     4081 cagactgggt ggtgatgact tcgaccagaa gatcacagac tacatgctgg ctgagttcaa
     4141 gaaaaatgag ggcgtagacc tgtcaaacga taaaatggca ctgcagagat taaaagaagc
     4201 tgctgagaaa gcaaagaaag agctttcttc tgcaacaacc acaaacatca atctgccatt
     4261 catcacagca actgctgaag gtccgaaaca tttcgatatg aatctgacaa gagcaaaatt
     4321 cgatgaactg acacacgatc tggtagagag aaccgctaca cctgttaaca ccgcactgag
     4381 agatgcaggt ctgacagcaa gcgagatctc caaagtactg ctggttggtg gttctacacg
     4441 tattccggct gtacaggata aagtaaaagc tctgaccgga catgaaccaa gtaaatctct
     4501 gaacccggat gagtgtgtag cacttggtgc ttccgtacag ggtggtaaac tggcaggcga
     4561 tgccggtgca ggcgatatcc tgttgctgga tgtaactccg ctgtcactgt ctattgagac
     4621 aatgggtggt gttgcaacaa gactgatcga gagaaataca accatcccga ccaagaagag
     4681 ccagatcttc tcaaccgcag cagataatca gacagctgtt gatatccacg ttgtacaggg
     4741 tgaaagacag tttgcgaaag ataacaaatc tcttggccag ttccgtctgg atggtatccc
     4801 accagcaaga agaggtgtgc ctcagatcga agttacattc gatattgatg caaacggtat
     4861 cgtaaacgta tccgcaaaag atctgggaac cggaaaagaa cagcacatca ccattacttc
     4921 cggatctaat atgtctgagt ctgatatcga caaagcagtc aaagaagctg ctgagtacga
     4981 agctcaggat aagaagagaa aagaagcaat cgacacaaga aacgatgcag acgctatggt
     5041 attccagacc gaaaaagcat tggaagaagt tggcgataaa gtagatgcaa atgacaagac
     5101 agcagtagaa gctgacttga acgaactgaa agacctgatc gcaaagagca atccggaaga
     5161 aatgacagag tcacaggttg ctgagatcaa agcagcaaaa gaaaaactga tggaaagtgc
     5221 tcagaaagta tttgcaaaga tgtatgagca gcaggcacag gcaaacggag cagctcaggc
     5281 aggtcctgat atgaatgccg gagcagataa cagcagctat cagtctgatg atgttgtaga
     5341 cggtgattac aaagaagtat aaaaaagtaa cttttaataa atttttgcat cgtaactgtt
     5401 aagatactaa acagttacaa tatacacagg cagggatgtg ttcattcctg ccttgtgtca
     5461 gcaatcaggc cgtaaggaac tgcaaacagg caggttctgg aaggttgccg tgcatgtgga
     5521 ggattaaaga tggcagagaa taagcgagat tactatgaag ttctcggcgt tgacagaggg
     5581 gcggatgaag ctaccattaa aaaagcatac cgtcagcttg caaaaaaata tcatccagat
     5641 atgaacccgg gagacaaaga agcagaaaag aaatttaaag aggcatcgga agcatatgcc
     5701 gtgttaagcg atgcagaaaa acgcagacag tatgatcaat tcggacatgc tgcgtttgaa
     5761 cagggcggcg gaggtgccgg aggattcggc ggctttgatt tcaacggcgg cgatatggga
     5821 gatatctttg gtgatatttt cggagatctt ttcggaggcg gtcgcagcag acgtgcaaac
     5881 aatggaccga tgaaaggtgc caatgtcagg acagcggtaa ggattacctt tgaagaggca
     5941 gtattcggat gcgaaaaaca gctggatctg aatctgaagg atgagtgtac aacctgccat
     6001 ggaacaggtg caaaaccagg aaccagtccg gagacttgtc cgaaatgcgg cggtaaaggt
     6061 cagatcgtat atacacagca gtccctgttt ggcacggtaa gaaatgtaca gacctgtccg
     6121 gactgtaacg gaagcggtaa gatcgtaaaa gagaaatgtg cggactgcca cggaagcggt
     6181 tacatcacaa accgcaagaa gatcgcagtg acgatcccgg ccggtattga caacggacag
     6241 agcatccgta ttcgtgaaaa gggtgaaccg ggtgtaaacg gcggcccaag aggggatctg
     6301 ctggttgaaa ttcaggtaga acgtcatccg atcttccaga gacaggatat gaatatttat
     6361 tctactgctc ctgtcacctt tgcacaggca gcactgggcg gacagatcca tatcacaacc
     6421 gtagacggcg atatggcata tgatatcaaa cctggaacac agacggatac caagatccgt
     6481 cttaaaggaa aaggtgtgcc ttctcttcgc aacaaaaata tccgcggaga ccattatgta
     6541 acacttgttg ttcaggttcc gacaaagctg aacgaagagg caaaggaata cctgcgtaag
     6601 tatgatgagg cagtcaacgg gaagattaac gatagtaaac aggaaaagcc gaaaaagaag
     6661 agctttatgg aaaaaataaa agaaacattt gaagactgac cctgagaaca ataaaaaagt
     6721 tgaaattcaa aaaatgcacc cgggaaagat ccgcggtgca tttttgtaca tgaaagttat
     6781 cggaaaggaa acattttcac cataaaaata cttgctgttt tgatatgcag aaacatgatt
     6841 cgtgtatgac gtaacagaaa cttaaggttt ctgacgttga caagaaagct tgaaaagaat
     6901 agaatatgat gtataggtat aactatagct ttatacatag atttggtgaa agcaggtaag
     6961 ttggatatga aattaaaatt actgaaaaag ttaatgatta tatgtatgtc tttcagcctg
     7021 tcagtttcca tggttccggc atctgtg
//
LOCUS       DAWDWH010000097         7026 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_4926_length_7026_cov_9.912208, whole genome shotgun sequence.
ACCESSION   DAWDWH010000097 DAWDWH010000000
VERSION     DAWDWH010000097.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7026)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4926_length_7026_cov_9.912208"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    989..998
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2815..2824
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3916..3925
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6138..6147
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1484 a         1786 c         1462 g         2254 t
ORIGIN      
        1 tgtcaccaat cgtaaaagaa taccgccgta ttcttttgcg aattacgacg gtattctttt
       61 tcggctttcg gaaaagcagt cgttattttc tttctgccgt tctgaaaata tttctcagtt
      121 tattattttt tctttgctgc ttttattttt actggttttg ttgtcttttc aggcctatag
      181 gttttctttt tcactgtcac gatacggtaa tagtatttca ttcctttttt cagtcctttt
      241 ttgtcggtgt atgtgcattt cgttcctttt ttcagcactg cgatcctctg atagctgcca
      301 tttttctttg tggaacgata tagcagataa ccggttgccc ccttcacttt cttccagctc
      361 agaactacgg ttttgtccgt tttggctttt gccgtcactt ttgtggtcgc ttttggaagt
      421 gtaatcttct ttgcactgcc ctctttgccg gtcagactgc ctttgatggc ttttgcatag
      481 taggaagacg ttccgcttac cggattctga tccaagaata cggttccgga tgtctgaccg
      541 atcatcgtca ctttgcttcc ctttttgcgg tagatctgat agccggttgc accctgtact
      601 gcctcaacgg tgatctgcac tgccgtcatc tttttcttgg caatgctttt aacgcttacg
      661 atctgcggag cctgaatggt ttctgaaggt gtcggcttcg gatccggggt cggatttggc
      721 tttggttctg gctttggatc aatgtccgga agcggatccg gggttggtgt ttcgttcttc
      781 tcccattttg cacagagtgt gatatctgtg ctaaccggtg tttcaaaatc atatttctga
      841 tcttccagat accatccaag gaaggtatag ccttctttct gcggtgcttc tttttcgctt
      901 gctttctctc cttcttttac ggaaacgctc tctttttctc cgtccgcatc aaaggtaacg
      961 gtataatatt tttccggttt gacgatgtnn nnnnnnnncc ccagcttcca tctgccgttg
     1021 atcttggctt tacctgatca agaatttcgg atgggatctt actataattg agtacttcaa
     1081 aatcggctgc attatcaccc gtatcgataa agctgatacg gcggatcgcc tttttcttgg
     1141 agatgatcgt atcattttcc ggatctgcca gtgcagttcc ttctgctgct tcttcattga
     1201 cagagacacc gtcaatctgc tcttcgcctt tcttcagcag gatgcggtac tgtttgttgc
     1261 tgatcgcaag atcccattcc tgatctgctt tctcgatcgt acagatcaga tctgcagaag
     1321 tcttctcttc ctgacctttg atcagataag aagtgtgaga tgtgaccgtt ccggtcagct
     1381 ccagctgttt ctgtgtgctt ccgctgaggt attcgatggt ataaccgctc agtccgatat
     1441 cctctgcggt tggattgtaa agttcaataa agcttgcaga taccggtgtt ttgccttttc
     1501 cgccgcctcc gtatacctga ttgatgatca gatgtgtcgt ctgggcttct ttttccacaa
     1561 cggctgccgt cttttcactg gtcagtgtgc ctgtttccac actgcttgtc actttcacga
     1621 aaatctgctt tccggtcagt cctttctcca cacttaaaaa tgcactgttt gcaccgggaa
     1681 tctctctttc gtctgccatc cactgataag acagtgttcc ggtattattg gtgatacctg
     1741 catcatctgc ataaagtgtt gctccgacaa tggcatttcc acggatggat acactgcctg
     1801 ccagctttgc cggggtcgga tccgggtccg gttttggtgt ctcaccgctg ccccatgctc
     1861 cgtctgccag gcttctcgga tatttctccg ttgcagcagt ggcatcgtct ttgtagctga
     1921 ctacctcaaa gtctgcggta ttgttgttcg tatctgcaaa tccgatacgg cggatcgctt
     1981 tctgcttgct gatcgtaccg tccgaaagtg tacttccttc tacatttcct tcgtttacgg
     2041 aaacggcatc ttcgacttta tttcctttat aaagcgttat ctgatatctc ttattgtcaa
     2101 tcgtctgtgt ccattcctgg tcaaatttgc ttaccgtaaa cttgatcaga tccggtgtgg
     2161 atgtatcctg ctgttcacat cggatcagat aggacgtatg ggacggtacg cttccggaaa
     2221 gtgcaagttt tacttcctgc ggtgtattac tcttgccgtt tgaaagatat ccgatgctgt
     2281 aaccttccag tgagattgca ttgtccgtcg gattgtaaag ttcgataaag ctgtggctta
     2341 ccggtgtgct gtcattggca ccgccgccgt atacctgatt gatgatcaga tgatcgtttt
     2401 tcagttcttc tttgatcacg gcatcggtcg gtgcactgac aaggcttcct gtttctatgg
     2461 agcttgtcac tgcaacggtg atcacttttc cgatttctgc ctctgtcagt gtatagttct
     2521 ctccgtttgc accctcgatt gcttttccgt ctgccatcca ctggtaagac agtgttccgc
     2581 tgttattgga atcctgtacg ccagcactta aaacggcacc gacctttgct tctccgctga
     2641 ccgtaacctt tccggtcagt ttttctgcgg cgatcgcctt tgtgaaatca acataattgc
     2701 tcaaagatcc gccggaaagc agattgccgg aggcatccat gcctcgtacc tgtacggtgt
     2761 tcagtccgcc ctctgtcctg gtcagaagat catcggatac ttcaaaaaca gcttnnnnnn
     2821 nnnntgatcc atccagtcca gacggttctt caggaaggtg cgaagccgtt ccacttcact
     2881 ctcaaatgta gagcgatgca tcagatagcc cgcctgataa ttggcatttc cggattccag
     2941 cagatattct ttctgctgat ccaggatacc gcctgtcttt acaatgtcct caaaattctg
     3001 acggttttct ttgtaacatt cgtaaagttt cgttacaaaa tacggatctc cgatcagcgt
     3061 cttataccag gaatttgcct gtgcttctgc cgaccttgtc acgaccatcc atgcccggta
     3121 attgctggtc tctccggcac tgatctggct gttggaggtc cagtccatat cccataccgg
     3181 tccgataaag agctttccgc cgaggtcttt gtacatgtag gtactctttt tcatggtttc
     3241 cgtgttccag taaaattcat ttaacatcag ataacggacc agagaatcca gatccaccag
     3301 atcggtgtag tgcttcttct ctccattgta ggtcgtataa taatcctcag aattgatact
     3361 gtcctcgaca gcctgtacat agttctgtgc cgctgtaaac agctcggtgt tcgtattggc
     3421 aaactccgga tttttgaaca tgatcggctg atttttcttt gtatggaatt ttgagacttc
     3481 gtcataatat gcatccagct caaacagata tccgccggat acgtctatct ttccgctgga
     3541 atctttcgga agatctgtgt agtaatcttc aatctgataa gtttttccat tataactgag
     3601 gcttccgctg gtgatccatt ccagatgttc agtcatataa tcttccagtg catcctttgc
     3661 gtcacccttg atgccttctt ttttggcgat cgcctttgcc gcatcacttg ccacatcttc
     3721 ccagttgtgg atattcactc tggatttatc aatccttacg tttcctacca gttgataatt
     3781 tccggcgtag acaccgttca ggatcacttc tacattggtg gatttcagat agaccatacc
     3841 catctttccg gacaggtcgt aggatgtctt gttacgcagc agagactcat ccatatagtt
     3901 tgccagaagt gcccannnnn nnnnngtgtt tgctgttgcc aaatccgagc agattggttt
     3961 tggaatccag tttgatctta tatggaagtt tggaatatcc catattccag gtactgtttc
     4021 cacgaccgcg gatggaaata ccgccgtcat aaagcgtggt gttcgtgctg ttaaagctgt
     4081 cattgccctg gatcttcatg gttgcctgtt tgtaggtctc tttgcttgta atgccatatc
     4141 catctttcgt attgatatag acaaccggaa gtttgctgca gtagatcttt ttctctgcca
     4201 cggtcgctcc ggtacttttc aggctcgcac gcactgtgat aaaggtattg agatccgcac
     4261 ttgtcggtgt ataactatca ctgtctgata gagtactctt atctttctgc catacataag
     4321 taaactcggt atctgccggt gcatttttta cagaagcttt catcagctgt cccggctttg
     4381 catactcccg gtcaaattca atctccagat cggatttgac ggttactttg accatctttg
     4441 tcttttgtgt cttcgatgtg gtcgcagtca gcgtcacttc ggtatctgcc agcacgcctt
     4501 ttacctctgc cctgcttccg tttattgtga tcacatccgg attgctgctg ctccagctga
     4561 tcgtctcttc tccgtcttct cctgcgtaag tcagcggcag ggtgaaatct ttggtgacag
     4621 aagatgcttt gtcaccgtcc gcaaacgtcg gtgacgggat cggcaccact acttttccgt
     4681 ccgtaacttt tggcataacc tgaccggatt tggaaatata ccagtaatcc gcaccaagtt
     4741 tttctgccag tgcttcactt ttcaggtcac tttcactgac ggaaccgccc tccgtatagg
     4801 caattccgct gccttccaga taataacagc ctgtaatatt tgctgtgttg gaggacgctg
     4861 ctgtattctg gctgtagcca ttgatcgcat aaccgccgga acctttgtat gtcacagttc
     4921 cgatattcag acaggaagta aggcttccga tctcaccacc ctgataaatc cttccgatga
     4981 tgccgccaca ccgcaccggt ggtgtggaaa ctgcagatgt cacattgaca gaagccgcat
     5041 tgtaaaggtt gctcatatgt acacctgccg tgttattgcg tgactgacca atgataccgc
     5101 ctacgccgat caggtcaccg ccgttgtttg ccgtcacttc gatgctgccg cccagcacag
     5161 caacgttgtc gatctttaca aatccgtcgg catctgctgc cagcgtgccg atcgtatctc
     5221 cgccgccaag attatggctg atggatacat ctgtaaatac aatatttttc acttccgcat
     5281 aatccgatgc atcatcgctt ccgagcattg caaacagtcc ggactgactg atgcggtaac
     5341 cgctgttgtt tgatccgtca cactggatcg taagcccgga gatcacatga ccggctccgt
     5401 caaacgtacc gttaaaaaca cgttcaccgg aaacgagggc atactgactg ccgccgcttc
     5461 ctccgatcgg tgtccagtca ctgccggaaa gatcgatatc atcggtgagc acatagtccc
     5521 cgtccatcgg atagtccttg tctttgccta tttttttcag ctcgtctgca tttgaaatcc
     5581 ctatctgccc cgatgcttct gccgcatgga cctgccacga tcccggaacg atcgtcatac
     5641 tggaaacggt catcaccaat gccagcccgg tcgcaagact cctccttaac aatttttgta
     5701 cttttttcat ttctacctcc tgatttttca ctctctcttt gttgtttttt actaatattt
     5761 tcattctacc acaggtattt ttgtttgtca tgtgaatttt agctgaaaat atcctgaaat
     5821 acttcgcctg gcattttata tgatgttggg gtcacagaaa atttttctta catttccgta
     5881 caccaaacaa aaaaagccgg aatatctttt atatattcca gcctttctta tctttttcat
     5941 ttcagctatc cttacacctg agccaggtca atatactgct cgatcagctg atcgactttg
     6001 atattctgac gatagatctc ttcggcgttt ccggacagaa tgatctcatc cagttttctt
     6061 cgctcacact caatactttc tttgattttt tccacttcgc tgcgtatttc gttcatttta
     6121 ctcatcgtac atccctcnnn nnnnnnnttc tgtgctttta cacggttttt tttgccgtgc
     6181 atctttttcg ttgaatcaat attactgcga tggagacaat ggctaccact gcggaaagca
     6241 cctgcgatac cggaagtttg atccccggaa gcagaagctg atccgtacgg agtccctcaa
     6301 tccagaatct tccgattccg tagcctgcaa gataaagtaa aaatatctcg ccgtcaaact
     6361 tcttatgctt cgtaaaaagc aacagcagca gcagaacgcc aatattccaa aggttttcgt
     6421 ataaaaaagt cgggtgcacc tgaatatact gtacgccgtc gatcgtctgc aggtgctcca
     6481 tcatctcctg cgtgacatcg gaactgcgta cggcattcag tggcagctgc atcgcaaaca
     6541 gaccatttgt atagccgccg aaagcctctc tgttgaaaaa gttgccccag cgaccgatga
     6601 tctgtcccaa aaccagcccg acacatgcgg tgtctgccat cagccagaaa ttttgttttt
     6661 ttatttttac atatacataa aatgtaatca ctgccgcgat cacaccgccg aatatcgcca
     6721 gtccgccttc ccggaaatac agaatgtgga tcagattgtc tttgtactga tcccaggaaa
     6781 agataacata atacgctctc gccccgatca gtgcgatcgg gatcgcatag agtgccacat
     6841 caaaatactg atccggattc tggtttgttt tcttcgccac ccacatggcg atgcacagac
     6901 ccagaatcat tccgcatgcg atgatcatac cgtaataggc aatctcaaag ccaccgatgc
     6961 tgatcgactt tccgacattt gataaatgaa tcccaagatt tggaaagttt atggatgtat
     7021 tcataa
//
LOCUS       DAWDWH010000098         6925 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_4987_length_6925_cov_14.889811, whole genome shotgun sequence.
ACCESSION   DAWDWH010000098 DAWDWH010000000
VERSION     DAWDWH010000098.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6925)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6925)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6925
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4987_length_6925_cov_14.889811"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2389 a         1296 c         1609 g         1631 t
ORIGIN      
        1 acaggatttt tattgttgga tgacagaaga aaatcaaaaa tcttatcagg ttttatcaga
       61 taatcaaatc tggtttttcc gtccggtcaa tgacacgcag aatagtcgca ttgccgttat
      121 tttcgcagag tacagcggaa gccgtctgat cggcgatcca ggagcagaaa actgtattta
      181 tattgaaacg ccggaaaaag aaaatgttat ttacagcaga aaattggaag aaaatcagaa
      241 aaacggatac ataaaagtag aaaaacagat gagtatgttt caatggaact gcgatatgta
      301 tgttccgaat accttgtttt atggagaact gagacgtttt gcaatgattc taacggcaga
      361 acttcttatg ctgatcagcg tggcggttat cggttcctgg tatttttcaa aaaaaactta
      421 tgtcccggtg ggaaatctga tcagtgataa caaaaaactt tcgaaaaaag ttaaaaggaa
      481 agaagagacc cgggaatgca gggaattgga aaaatatctg acaggtgtgg tcaaaaattt
      541 tccatatgca agtctgaata aactatcaga tcaagaaaag tatataatga tccagtttgc
      601 ttttgatgga aatggtgatg cagttttcag agatcaggaa aaaaaggaga aaccggaaga
      661 gctggagcat ttcgtgcttg aaaatatttt gaaagagcag atttttgaaa aatatccagg
      721 aatccttttg cagccggaaa aagattttgt ggcagttgtc aatctttcag aaccggaaca
      781 tgatgaaaaa gaaatccgca gtctgctgaa gacggtagaa cagttttaca gccggacgtt
      841 tcatatttct gcctgtatca tgatcggaga atgtcagagc gactacaaaa agatgaaaga
      901 agcgtatcat cttctggaag agagtatgca gtatctggag ttctggagta cgagtgcacc
      961 tgccggagtc tatgtatatg gtgagatgat ggatgcggat gaagacagtc attcctcggt
     1021 ttacatgaca ggcagcagaa gacttctgaa ttgcctggag agtgaagatt ttgaaggagc
     1081 ataccgggag cttaaccaga tttaccagag tgcattttcc agaaatcaga aagagctgag
     1141 gattaatcgc tatcgcatgt atggtctgat tggaaccctg atcacgacac ttgatatcag
     1201 tgcaaatgaa gaagacaaag cttattatca gtcactgaat tatgaagagc gtctgtttcg
     1261 tatccagtcg atccatgagc tgctgtcaga aagcagggca atttttgagg cgatcattca
     1321 gtacagacag aataaatctg cagaacgaga accggagtgg atggaagaaa tgcaggaata
     1381 tattcagggg cattatcagg actgcaatat gaacgtgacc agtctggcac aggaatttgg
     1441 catcagtgta ccacacgtaa gccgcagctt taaaaacttc cgagattatg gcgtactgga
     1501 gtacattcat aaagtaaggc tggaacatgc aaaggatatg ttaaaaaatg atgtacgcat
     1561 caaaaatata gcacttgacg taggttatac cgatgcacag gcgtttacaa gagcctttaa
     1621 acgctatgaa ggcattactc catcgcagta taaagagctg atatcaaaaa atgaagcgta
     1681 aaatcaaaaa ctgcttcttt aaaaaacata ttgtttttcc tatactgaga gcacaaacaa
     1741 agcaccggat aaataaggtg tttccagaaa aaggaggaaa aacaaaacat gaaaaaagca
     1801 acaagaagta ttttgagtgc cgctgttgct gtcagtctgg cagcaacggc atgcccggta
     1861 tcttttgcgg caactacttc caatgaagtg acagcaagag aaaaagcaaa tgcagcactg
     1921 gcaagagaag cagctgcaca gggaatggtt cttctggaaa acaagaataa ttctcttccg
     1981 attaagacaa agaaactggc attgttcgga ggcggagctg tacgtaccgt ccgaggtggt
     2041 accggttccg gtgatccgtt taacggcgga ctttccggtg gcggtgatgt gaatgtaaac
     2101 cagagtgaga gatataatat taacatttat aattctttca aaaaagcagg ttacgatatc
     2161 acgacggcaa gtattcttga aaaatatgca gaaggatacg atgtagaaaa tgcaaaagca
     2221 gcaagcaatc cgatggcaac ctttgcttat ccggaactta ccttcacgga tgaagaactg
     2281 gcagcttctg caaaagatac caacactgct gtttatgtaa tctcaagaaa tgccggtgaa
     2341 ggtgctgacc gtggaatgac caaaaaggtt gcggtaaata atgtagaata tgaattaggt
     2401 gactatgagt taagcgatgt agaaaaagaa aatctgaaaa aggtagcctc tgcttttgaa
     2461 aataccattg ttgtactgaa tgtaggcggt gtcattgaca cgaaattctt cgaggaaaca
     2521 gaaggtctgg attctctgct tctgatggga cagggcggtc aggaaggcgg aaatgcactg
     2581 cttgatgtag taaccggagc tgtcacacct tccggaaaac tgacagatac ctgggcagaa
     2641 aattattcag actatcctgc atctgccacg ttcgcaaaag cagacggtga tagtatgaag
     2701 gaatggtaca aggaaggaat ttatgtagga taccgttatt ttgatacctt tggcatcaag
     2761 ccggcatatg aatttggtta tggtctttcc tatacaaatt ttgatatcaa tgtaaagaat
     2821 gtatcggtaa atgaagataa ggtgacggta aaagcagaag ttacaaacac cggaaaaact
     2881 tacagtggaa aagaagtagt acaggtttat ttctccgcac cagactcaaa agatgcagaa
     2941 aaagaatatc agcagctggc tgcctatgga aagaccgatg aactggcacc gggtgaaagt
     3001 caggtactga cactgactta tgataccgat gagatggctt attatagtga agaaaaggca
     3061 tcttacatac ttgacccggg aacctactat gtgcgtgttg gtgattcttc cagaaataca
     3121 aaagtagcgg cagcgatcaa actgaatcag agtgcagtga cagaagttct gtccaatcag
     3181 atggaagttc cggaaagcga aaatctgacc gagtggagca aggcaggaaa aacaccatat
     3241 acttatgcga cagagcagca ggaaatggca gaagccccgg tatttacact ggatgcatcc
     3301 aaagtaaaaa cagaaaataa tgtttctgaa tataaagatg aaaaagttac tacttacaca
     3361 acagatccag actataaggc agttcaggat tacgaaaaag tagaagttgt tacagacaaa
     3421 aaaggtgcta ctttaaaaga tgttgtggat aacaaagtaa ccatgggcga atttgtggca
     3481 cagatgagtc tggaagaact ggcaaagctg aactgtggaa gcggctgggg agtagcaaat
     3541 gaaaatgctc cgatcgttgg ctccaactca gcaaccgtac cgggagcagc aggagaaacg
     3601 ctgacttatg atcagtatgg tattccgagt atcgttttag cagatggccc tggtggtatc
     3661 cgtgtaaaac agaaatacga ggcaaaaaat gtagaaacag gagaaacagc aacttactat
     3721 cagtactgta ccgcatggcc ggtagatttt gttctggcac agagctggga tacggatctg
     3781 ctgaaacgta ttggtgaagc atttggaaaa gaactggaag agatgaatat tacaattctt
     3841 cttggaccgt ccctgaatat ccacagagat cctctttgcg gacgtaactt tgagtatttc
     3901 tcagaagatc cggtcatttc cggtacgatg gcatctgcaa tcacacttgg tgtgcaggaa
     3961 gagccgggtg taggggcatg cctgaaacat ttctcagcaa ataatcagga aacagaccgt
     4021 tccggaacgg attccattgt aagcgaaaga gcactgagag aaatctacct gaaaggtttt
     4081 gaaattgcag taaaagaatc caaaccaatg tccatcatga catcctacaa ccagatcaat
     4141 ggcgttccgg cggcagactc ttatgatatg aataccaata ttgcacgcgg tgaatgggga
     4201 tttgaaggtc tgatcatgac cgactggaac ggcggtgtat ctcacccatc cacatccatg
     4261 catgcaggaa atgacctgat catgccgggc ggagcatcca aagcaaacga aattatcata
     4321 ggtgcagaag atgtaaaacc gacctttgaa gcaaacggac agattggttt gaaagatgaa
     4381 ctgatgtata tgttcagtta taaatcagca gcatggggcg attttgaagt ttcagcggat
     4441 ggaacacaga cagcggaagc aaaactggga gatgactaca cagcaagtgt aggtgaggat
     4501 ggaaagattc tggtaaacgg acaggaaatt tacagagagt atcaggcaaa tgtctgggct
     4561 ggaacaggaa actataaaac accggttacg acagatgtgg catctgtaag cgaagatgga
     4621 aaaaccattg tttataaagg tacttacaaa gaaaacaaca atatttgcct tggtgatgtg
     4681 cagaagagtg cgatcaacaa cctgaaagtc attatgcagt caagagatat ggcaagaatt
     4741 tacggcacaa caacaagatc ttacacagca ctttgcggtg atctgacaaa ttatcagaca
     4801 gtaaataaat cagagatcac aacagaacgt actttaaaag aaaatctgga tcaggcaaag
     4861 aaggatcttg aagaagcaaa gaaaaatctg gaagaactgc agaaacagaa tgcagcaaaa
     4921 gatgcagaga ttgcaaaagc aaaagcagaa gtagaagcag caaagaaaga tcttcaggca
     4981 gcaaaaatgg cagcaaagaa agcagccgtg aaatctgtaa agagtaaaaa agccggaaaa
     5041 gcaatcgtta ccgttaaaaa agtagctggt gcagaaggat atcaggtgca gtattccctg
     5101 aaaaaatcca tgaagagtgc gaaaaatgta aaaatcaaaa agaacagcat aacaatcaaa
     5161 agactgaaaa aaggaaaaac ctattatgta agagtaaggg ctttcagtaa agactccaat
     5221 ggcaaacaga tctgtactgc atggagtact gcaaaaaaag taaccatcaa aaaataatac
     5281 aaatctcttt tcatcattcc tatataaata cgaaatatcc cacagatttt tctgtgggat
     5341 attctgcatc tgaaataaat tatccgaagc attcttgaat cttatacgat gccggttgaa
     5401 acaagaaaca ggatcagcat cacaaccggt acagcatagc ggatcataaa acaatacaat
     5461 tttgcacgtc tgaaatgttc gccatttcgt tctacttcat caatgatcag cttaggccct
     5521 acaacccagc cgatcagaat actggtcagc agtgagatga acggcatcag gaagctgttg
     5581 cttatgtaat ccatgatatc cagaagctgt gcgacggttt cgtttggaag ttttacttca
     5641 aaataaagta cattgtagcc gaggcagatc agcactgcgg taacaagaga ataaattccg
     5701 atgacagcac acattttctg gcgtgactta tggaaaattt ccatgcagtt tgcgaccaga
     5761 gtttccataa ccgatacaca ggaagtcagg gcagcaaagc ccatcatcag gaagaaggca
     5821 atggcaatga ttctgccgat cgcacccatg gatgcgaata cttttggcag cgaaataaag
     5881 atcaggctcg ggccggaagc cattccgtct gttccaagaa atacaaacac agccggtatg
     5941 atcatcattc ctgcaagaaa tgccacgccg gtatcaaaaa tttcaatctg attgattgat
     6001 ttattcagat cgacatcatc tttcacatag gagccataag taaccatgat tcccatcgaa
     6061 acactcaggg agaaaaataa ctggctcatt gcatcaagaa gcacaccaag aaaacgtttc
     6121 acggtcagcg aactgaaatc cggatataaa taaactttaa gtccctgcag tccggtgcga
     6181 accgttccat ttgcatcggt atacgaaagc gtcagagaaa aaattgcaat gccaaggatc
     6241 agcaggatca gtccaggcat aatgaaacgt gaaaattttt cgattccctt ttccacacca
     6301 cggtatacaa cccaggcagt taaagccaga aacagcagca tgaatacgat cggcgaaaac
     6361 ttggaagtga taaaggatgt aaaatagctg tcctgtgaca gtatcgaacc atctgagaca
     6421 acataggtgc agaaatattt ggtgatccat ccaccaataa cggaatagta agtcatgatc
     6481 aatgccggaa caagaaaagt caggtatccg agaaatttcc attttgaatt caaagttccg
     6541 aaggctttca atgcattttg ttttgtgtgg cggccgatgg cgatgtcagt ggtcagcaat
     6601 acaaatccaa aagacagcac cagtgcaaga taaacgatca gaaacagacc gccgccatcc
     6661 tttgcacata gatagggaaa acgccagata ttccccacac cgacagcact gccggcagcg
     6721 gcgaggacga atccgatcga accactgaag ttactctttt tttctttcat ttcattcttt
     6781 acctcgtttt acttttttgt ttgaacgcag agaagcattc ccccgcttca agtgtgcaga
     6841 gcactaagcg gattgtgaat gtccgcttta cattcaagcc gaacgcacgt gagacttgta
     6901 gcacatgaca tatttttccg cacac
//
LOCUS       DAWDWH010000099         6902 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5000_length_6902_cov_9.392873, whole genome shotgun sequence.
ACCESSION   DAWDWH010000099 DAWDWH010000000
VERSION     DAWDWH010000099.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6902)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5000_length_6902_cov_9.392873"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    909..918
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3020..3029
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1654 a         1801 c         1476 g         1951 t
ORIGIN      
        1 cgtttagatg cacgcacgct cggcgtacca tcgtatttat cgcagtcgta tactacggtt
       61 cggatcccgc actgaatgat cgctttggca cactcgttgc acggaaagag cgaaacataa
      121 agctttgctc cctccaggct gcctccccgg taattgagga ttgcattgag ttcgctgtgc
      181 gttgtataaa aatacttggt atcgtacgga tctccttctc ttctccacgg gaattcatca
      241 tcggaacatc cctttggaaa tccattatat ccaatggaca gaattttatt atcctcgctc
      301 acaatacagc agccaacctg tgtatttgga tctttggatc tgagtgctgc aagatgtgca
      361 acccccataa aatactcgtc ccaggaaata tagtcggttc ttttgtccat actttcctcc
      421 aaatgacgtt ctccgtcaga ctcaggcaac gtctattttg tgccgaagat acggtctcct
      481 gcatcgccaa gtcccgggac gatatagcca tgggaattga gctgttcatc caatgcaccg
      541 atgtacatat ccacatccgg atgacattcc tgcatcttct ttacgccttc cggtgctgcg
      601 atgatgcaca taaaatggat gtgctttacg cctttgtcct tgagcatctg gatcgccgca
      661 gttgcagatc cgccggtagc cagcatcggg tctacgacaa aaacttcacg ctcttcagaa
      721 tctgccggca gtttgcagta atattccacc ggctccagtg ttttgggatc acggtacaga
      781 ccgatatgac cgatctttgc agacggaatg atggaaagca taccgtcaac catgccaaga
      841 cctgctctca gtatcggcac aacagccagt tttttaccct gtaattcttt tacggttgcc
      901 gtgcagatnn nnnnnnnncc gtcagtttca gttctctggt tgcctcatag cacatcagca
      961 ttgcgatctc actgatgatt gtacggaaat ctttgctgca ggtgtctttt cttctgatca
     1021 ccccgatttt atgctggatc agcggatgat ccataacttt tacttttgac ataacgttct
     1081 cctcattctt acttcctgtc ttttctctta tttgtcttcg atcatttcga tacgtctgat
     1141 gtgacgctca tccccggaaa atggggtatc aaggaacgta tctacgatct tcagggcaag
     1201 tcccggacct accacgcggc cgcccattgc cagaatgttg gcatcgttgt gcagtctcgt
     1261 tgcctctgca cagaagcagt cggtgcaaag tgcagcacgg atgcctttta ccttgttggc
     1321 tgcgatggaa ataccgattc cggttccgca gatcaggatt cctttctcaa attctcctct
     1381 tgcaacagcc tctgccactt ttttgccgta gatcgggtaa tctaccgact ctgtgctgct
     1441 gcagccgaaa tcgtggtatg caattcctct gttttccaga tgcttcttta cttcctgcat
     1501 cagttcgtaa ccgccgtggt cacatccaag tgctaacata gtagttcctc ctcattcaat
     1561 acaacaacca ggctgctgat catgcagtca agcagctcat agcaggctcc gtagtccgca
     1621 agactcccgc ccaccggttc tgtaacatca gtttcttctt ttatatattc ggaaagctgc
     1681 caggtgttct gaacattctc atgctcctgg aaaatcctct gtttgagagc gtcttccatc
     1741 acaaggatca gcgtccgctc atcaaaatct tcctgttcca gcatcttcgc cgtatgatct
     1801 ttcatggtca gcccgtggct tgccaggatt gcttctgctt tctgattgac cggctccgga
     1861 aagagtacga caagtcctct ggactccacc tccagctctg agagcagaaa tttgcttttc
     1921 agaatcgctt ccgccatcgg tcctctgcag gtatcgctgt tgctgacaaa gatcagtctg
     1981 tcgtatttct tcataggcta tacctccatc acctgatggc ctgctgcttt catcagacgg
     2041 ttcatgatcg cctgccccat ctgcggtgtc tcaaaactct ctgaatagat gcgatccacc
     2101 tgaagcgtgt caaaatctct taatatgcca aacagatggc gtgaaatgct gagctcatcg
     2161 ctgcggctgc cgatcgtgcg gacaattccg cccaggtagt ctgctgctgt ctccgttgaa
     2221 gcgatcacgc cgatcttctc gcctgccgct tctccttctt ttaacagttc gttgatcttt
     2281 gccgtaacct tttcctgctc tccctcaacg atcgtcaggc ttgccttcgg tgcatagtga
     2341 cggtacttca tgcccggtgc ctttggtttg atgccgctgt catccgagag gatcgcacgg
     2401 tcgatctccg tctttccgat cacttcttct aacatctgct gattgatata gcccggacgc
     2461 aggatcgtcg gaacgccctc gctcaaatct acgatcgtag actccagccc gatccctacc
     2521 ggacctccgt caaggatcat ctcgatcctg ccgtccagat cctcctgcac atgtgccgca
     2581 caggtcggac ttggccttcc ggaagtgttg gcactcggtg ctgcgatata accgcccgct
     2641 gcccgtatca atgccagtgc caccggatga tccggcatac gcactgcgac cgtattcaat
     2701 ccgcctgtcg tccccatcgg aaccagatcc gtcttttgaa aaatcatcgt aagcggtccc
     2761 ggccagaagg catccgccag catttttgct tcttctggaa tttctgccgc aattttgttt
     2821 aatgcttcaa attctgcaat atgtacgata agaggattgt cggaaggtct tcccttcgca
     2881 gcgtaaattt tctccgccgc ctgttcatcc agtgcattgc cgccaagtcc gtataccgtc
     2941 tccgtcggaa acgccacaag accgccgtct tttaagatgc tgccggcttc tgtaatgatc
     3001 tcctgatcga tcccttcttn nnnnnnnnnt cttcataatc ctgcttccgt cctttttctt
     3061 ctaaaaacct tctctgcgtc tctgtttgcg gagtgcatag cgtttctgtc ctgcttccca
     3121 ctctgccttt tcactctcgc tctcaacgat cagtcccggg acttccactg catgtccctc
     3181 ctcgtcgatc gccaccatca ccacataggc gcggttgatc acgcgacgca ttccttcaag
     3241 atcctcgaca taagtatcga cacgtacctc catcgaagtg cgtcccacat aagtgatacg
     3301 tccgatcaga acgatcgtat catttaaata agctccggct ttgaagttga ggttgtcaat
     3361 gctggccgtc gtgatccgtc ctcccgcatg ccgcatggaa acgatccctg ccagttcatc
     3421 gatccactgc atcagctgtc cgccgaaaag ccttccatat ccattgatat ggcggtgcat
     3481 cagaagatac gtctgctctg tcatagaatc ctgaactctt tttttcttat ccaaaatgat
     3541 caccccggat tattcgtcgt ctctgaagat cacgccgtct ttgccaaggg tacggaaatc
     3601 catatcgatc atgcgatcaa acaggtcatc ctcatcgtct tcgtcatcat catcatcaaa
     3661 atcgtcgtca tcaaagtctc tgtcctgata ttcataggca cttaaatctg ccttgccgtt
     3721 agcatacagc tcacggatcg ctttttctgc tgattttgca tcgatggcaa ctgcgatgtc
     3781 ttcctccggg ccgcagatct ccatgagttc ttcgtcttca atataggcag ctttggtcac
     3841 ttccataact gccagaaatc cttcttcatc tttatataat acctgcatat ctgttcctcc
     3901 tgtctcgttg gtatcattac gttacgcttt ttttattata acacaagaaa gccctgtgca
     3961 aaaccctatt ttttgtgaaa taaaaaggaa gccagacttg ttacaagtct ggcattagga
     4021 aagagaaaaa atgaaatata tgtaaaaaag gaatgaacct ttgtacaagt actatcataa
     4081 cggcattctg tgaataaagt gtgttcgaac ggtgaaagta ttgttatgaa attctaaatc
     4141 atttccaaac attctaaaca aaacagcagg aaagtgtgta cattcttgtg aagaattgtt
     4201 gacgaaccct gtttccttgt ttataatgaa ctccggttat tgttacgccc gcagggcgtt
     4261 tttggtacaa aatactgaat taggaggaca ccatgaacaa gtcagagctt atgaaaactg
     4321 caaatgaaat ccgcaaaggt gcgatcaccg gcgtttattc tgcaaaatcc ggtcatccgg
     4381 gcggttcttt atccgcagca gacatttaca gttatctttt ttttgtagaa ttaaatatag
     4441 atcagaaaaa tccgaaaaaa ccggatcgcg atcgtttcgt cctttccaaa ggacatgctg
     4501 cacctggtta ttacgcagct cttgcaaacc gtggatattt cccggtggaa gatttaaaga
     4561 cactgcgtca cattggttct tatcttcagg gtcatccgga tatgaaacat atccctggtg
     4621 ttgatatgtc aaccggttct cttggacagg gtgtttccgc tgccgttggt atggcaatct
     4681 ctgccaaact ctccggcgat gattaccgtg tatatacact gcttggtgac ggcgagatcc
     4741 aggaaggaca ggtatgggag gcagccatgc ttgcagccca cagaaaactg gacaacctgg
     4801 ttgtgatcgt agacaacaac ggtctgcaga ttgacggtaa gatcgacgat gtaaactctc
     4861 cttacccgat cgacaaaaaa ttcgaagcgt tccgtttcca tgtgatcaac gtagacgggc
     4921 acgactttga tgcactgaaa caggcttttg atgaggctcg caccgtaaaa ggacagccga
     4981 ctgccatcat cgcaaagacc atcaaaggaa aaggagtctc cttcatggaa gatcaggctt
     5041 cctggcatgg tgttgctcca aacgatgaac agtacaaact cgccatggaa gaacttgaga
     5101 aagcaggtga agcattatgt cagaagtaaa gaaaatcgct acaagagaga gctacggaaa
     5161 tgctcttgtt gaaatcggaa aagaacatga agatctggtt gtactggatg ccgaccttgc
     5221 cggagctacc aaaaccgcta ttttccagaa agtattccca gaaagacaca tcgactgcgg
     5281 tatcgcagag ggcaatatga tgggcgttgc agctggtctt gccaccaccg gaaaagttcc
     5341 gttcgcaagc tcttttgcca tgttcgcagc aggccgtgct tttgaacaga tccgcaactc
     5401 catcggttat ccgcacctga atgtcaagat cggtgctacg catgccggta tttctgtcgg
     5461 tgaagacggt gccacacatc agtgcaacga agatatcgct ctgatgcgta tgatccctgg
     5521 catggtgatc atcaaccctg ccgatgatgt ggaagcaaga gctgccgtaa aagctgctta
     5581 tgaatatgtc ggaccggttt acatgcgttt cggacgtctg gcaatcccgg tattcaatga
     5641 tgagagtacc tataaatttg agctcggcaa aggtgttgtc cttcgtgaag gcaacgatgt
     5701 tgcgatcttt gcaaccggtc tggaagttta tgaaagcatg caggctgcag aaatgctcgc
     5761 agcagacggc atcaacgcca aagtcatcaa catccacacg atcaaaccgc tcgatgaaga
     5821 actggtagtc gcttccgcaa aagaatgcaa aaaagtggtt accgttgaag aacactccat
     5881 catcggtgga ctcggcagtg cagtatgcga cacactctgt gcaaactatc cgactccagt
     5941 attgaaaatc ggtgtcaacg atgtatacgg cgagtccggt cctgcagtac agctggtgaa
     6001 aaaatacggt ctggatgcag agagcatcta caagaaagta aaagcttttc ttgcgtaaac
     6061 tctttaaaca aaaggaagaa aacaaaaacg actttccgct atggtttttc catttggaaa
     6121 gtcgttttta tttttctgtt tttcggtctg tctgtgtaaa atacttttta ttttagatag
     6181 ttcggataga aagaatctgc agatacatcg gatagtagcc aagtccgtaa acgccccttg
     6241 cccaaccgtc aaactgtgcc tccggatcat agtaaagtcc gttgatcttg accagagaat
     6301 gtctggtaaa cccatccggt gcaccatcta cgccgcccgg gacacggccg gcaatgacct
     6361 gtggctgata gccgattgct ttggcgagca tggcaaatcc acatgcgaaa ttgtagcagt
     6421 tcccggctct cttttcgtac atttccaagg catagtcgta ttcccatccg tcatacacag
     6481 agaatcctct ccaggtacgg taactgaaat tcgcacggct tgtcatatac tgaaaagctg
     6541 cgtaaagctt ctggctctta ctcattttag aagttgtgat cttagcaaga atctgcttgg
     6601 ctttctccag ggctttctgt tccttcagct ccgtcttgct ccgttttaca tagccacttt
     6661 ttccgatctt gacaccgtct acttttttat tgacctgcat tcttccgctc ttcttggaaa
     6721 aatatgcttt tttgctgccg aaaatctgcc agccggtctg cattcttccc ttcttatcaa
     6781 acgaatacct gaattttccg atctgcttta ctttattctt tacttttttg cccttctgat
     6841 tgtagtaata gcgatatatg ccttagtata cccactgatt tataattgtt gccgcatttg
     6901 ca
//
LOCUS       DAWDWH010000100         6836 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5038_length_6836_cov_8.693998, whole genome shotgun sequence.
ACCESSION   DAWDWH010000100 DAWDWH010000000
VERSION     DAWDWH010000100.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6836)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5038_length_6836_cov_8.693998"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2014..2023
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2488..2497
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3584..3593
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4814..4823
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1403 a         1699 c         1433 g         2261 t
ORIGIN      
        1 acgaaataat accatccaga ataattggag tatgaaaact caccaagccc ctgcgtatct
       61 ttatacggat tcgacttgtg tttgtcattg gtcggtgcat cttctgacat atttgaaata
      121 cactgcggaa tattcagtac accgctgtct gcatggtcga tctctgccag atagaatccc
      181 attccggtgt tctgtgcatc gtaagtatag ccgtttgctt tcagaagttt ttcgatgata
      241 tctgcataag tctctccggc ttcaaaggaa acttccgtcg gctcgatcag atagccctgt
      301 ccaacggtaa atttttctac ggatgcataa acgattccgc ccttgtccgg tacaaatgtg
      361 gatttaccac tgtctgcgtt gtcttcgctg tctttaaacc agatctttgc ttctttttct
      421 tctgctctga ctgtctgcat accggatgac ggcagaaatc ccgtcaggca taaaaccata
      481 gcaagcagag cagcaataat actctttgtg catcttctta tattcaaaat atggactcct
      541 tcctataaaa caggaagaag tatggaacac tccttctgcc gaagagcgta tccatactcc
      601 tgtttttgtg tttctctgtg actttatttt accttaactt tacgtatggc agaatatgca
      661 ccagtaatgg tctttccgtt gactttttta tatgcacgta ctttgtaata gtaagttttg
      721 ccctttttca gctttttgtc tgtgtatttt gctgccttca ggtttttctt cacgcaaacg
      781 tatttgccgt ttttacgcat ggaacgatat acctgatatc cggctgcacc cttgactttt
      841 ttccagatca gagttgcttt tttctttcct gctgctgcct ttctgatcac aggtctggcc
      901 ggtttcttag ccggtttttc tgtggtcggt gtttccggtg tcttatccgt ggatggcgtt
      961 tccggtgtag atggcgtctc cggtgtgccc ggtgtttccg aagtagacgg cgtctccggt
     1021 gtgttcggtg tttccggggt agacggcgtc tccggtgtac ttggtttcag ggagttcata
     1081 tcccagtagc cttcttcgga tgctctaaat cttctgtatg cttcgtatgc ttccatgatc
     1141 tggtaagaag ccattgcatt ggcaacacca tttttcagat gtgcataacc gccgcctttc
     1201 acgcagtaat ttttctcaag cgttgagaag atactttttg attctgctgc aaaacctgca
     1261 tccacggcat ccagtttcag gcaggcaagt gccaggataa cctgtgcagc agattcggaa
     1321 gaagtataag cgtattcttc gtccatagca tctttcagat atgcaagtgc tttgtcaacc
     1381 gccgtctgta ctttcgcttc ttttacatat ggtgccagtg cctgcaggat catagcagtc
     1441 atatctacac ctgttgattt gttgtcaaac aggccaaaac caccgttttc attctggaat
     1501 ccaagcagtg catcgatcat ggactctctg ttccattttg catctgtagg aattttctga
     1561 gaggttgcat ccagtgcgat cagtgtccag ctcagttcgt ttgaaccgtc ttccagtctg
     1621 tcgctgctgt acagcattgc tgcaagattt acaccgccta cattcgtgat atctttgccc
     1681 atagcagaca aggtcagtgc cactcttgcg atatcggtcg gtttctggtt tgctttccat
     1741 tttgaaacaa cagaaacagc actctgaccg tatgcttctt tttctgcatc gctgatcgtc
     1801 tgtccggtac gcagttttga catgatatac cagtcggatg caccgctgca ttcgtattta
     1861 tagttctggc tgttcaggaa ggtggatgca cttgtgatac cttcttttac aatctgatct
     1921 atatcgactt tgtcggatac ctggctgtta tctgtaactt caccggcctt ccgtgccttg
     1981 atacgatgtg atgtttcctt cggcgtcagt atannnnnnn nnngtaagtt ttgcctttct
     2041 ttactttgct gtctgtgaat gttacggcag atgctttttt gatcgttctg atctttttgt
     2101 acttgccgtt tttctttgtg gcacggtaga tttcgtatcc ggttgctttg ctgttcttct
     2161 tccacttcag gaatgctttt ttctttttgg aagatacggt cagttttgtt acttttgccg
     2221 gtctgacaat ggtctgtgtg cctggattct gatttccgtt tatgccgttg tttgtattat
     2281 tactgttgtt gttattgctg ttatcgccct tgctgttgtc ctttttatca tcgtctttct
     2341 ttgcatcctg taaagccttg agctgtgctt ccgcttttac cagcagatct gcattttcca
     2401 catttgcttt ctgtgtatcg gtaagtgcgt cgtatgctgc gcttgcggct ttttatgctt
     2461 cctgagcgtt cggtgtaagc atatcgannn nnnnnnnttg tctgtatagt gcagcatgac
     2521 aacgtcaccg tcttttaatg tataatcaca tgcaccaaca tccggttccg ttccgttcac
     2581 gctatatttc cagccggatt tgcttccgtt tgtaaactct ccgagtttca ccccgtctgc
     2641 tcttgtaacc gatgcaatgt aagtgtcacc gtattttttc tcactggtca ttttattggc
     2701 cgcaagtgcc gcatccagag cttccatggc ggttgcattc ttcggaacac gatactcgct
     2761 ctgtgctgcc catacctgaa ggttattctt tacaaatgca tggatctttc cgtctttgtc
     2821 gctgtcgtgt acttcatcgc caaggatggt gacagccacg ctctgatttt caccatttat
     2881 cagccagtag gtgtttgttc cttttttata tgcatcaagt gcctgcagta cctgtaccgt
     2941 tgacatttcc tgcgattttg taaggttaga cagatgtgaa aatcctccgt cattctggcg
     3001 gtatttcatc agatttgtga tcatattaaa gtttggtgta ccaaatccca cttctacaga
     3061 agtcggatca atgccaaggc aggaaagtgc cagaagtacc tgagccgtag attcggaagt
     3121 tccatatccg aaatcatcac gcatctcctc ttttaaacag gaaactgcac gatcaatcgc
     3181 ttcttttact tcggttcgtt cctgataagg tgcaagggcc tgaagtgcca tggccgtcat
     3241 atctacactg gtgctgtctt ttccggtcag tccgaatcca ccgttttcat tctgaaaacc
     3301 aagcattgct tctatgatcg tgtttctgga ccattttgca tccgccggaa tcgcagcatt
     3361 ggctgcatcg agtgccatca gtgcatagat cagttcattt gatccatcgc tcagcttttc
     3421 ggaattgcag atcatggata ccagatctgt accgtccaca tcggtgatat ctttgcccat
     3481 tgccgtaagt gtcagggcaa cacgctcgat gtctgtcggc ttctggtctg cattccattt
     3541 ctttacttcc gctgcagcag actgataata agcatcctgc acannnnnnn nnnctcaact
     3601 ccattacgaa gcaatgcgta gatcagccat tcatctccat acgcatagcc gtctgtgctg
     3661 tgtttttctg tgatgtacgt tacggcactg ttgatgcctt cttctgcttt tgcttttaca
     3721 tcgatatctg cggtgcttcc ctttgttacg gtaatggtga tcgttaccgg ttttacgtta
     3781 tttttgccat cgatcggtgt tccggttgct gtcacggttc cttcagagag tcccgtaatc
     3841 tgatattctg ccaccggaag ataggaacca taatccggat cgttcacatt ataatcgccg
     3901 ttctttttcc agtaaccgct tccggttctc acaacctgtg caatacctct ctggctgtaa
     3961 gtccatttca gagccggttc cgtgacatcg tatccctgat catccagttc acctgtgaca
     4021 attggtgcaa acactgcact gctcttgccg gcttctaccg tcatattctt attttcttct
     4081 gcaagttcca cggtttttac gttgtttttg taaacaaagg ttaccgtact gtcaccggaa
     4141 actgttttcg tttctcccgt ctgcggattg gtatcttcga ccgtcgctgt aaaggttacc
     4201 gttccggcct gtttcggtat gtaagccttt tcgccatttg tatagtatgc gatcgttccg
     4261 tccggatcat cacttgtgat cgttacttta tctgcattgg tcgcatttgc cggtgtgaca
     4321 actgccgaac caaggattgg attgaatgca actctgccat ccgtttcctg tccgtcgctg
     4381 tttgcatttc ggctgtgtat ttcttttgtg ccggaaattg ccggaacaac ggatgttacc
     4441 ggaacataag cagaggttac cgtcacggtt ttgtgaattt ccggctgatt ttttgcagtt
     4501 actgtaaaga ctgcacttcc ggtctcttta aaggaaaagc cgctggaaga atcggtgcgg
     4561 ttatgtagaa gttttgcacc cgcttcatcc gctgcataga taaaggaagc gtaagaaaca
     4621 tcctgatact cttcactgcc ctgatactgt gcttttacgg taatatgctt gtattcagaa
     4681 ccctgtaccg taacttttcc gtccgttaca tcttctccat ccacatacag tttcatatct
     4741 gtcatttgga ttctgctgga acggatcgct acagatgcca ggatttcctg actgccgtcg
     4801 ctgttgttct gtannnnnnn nnntacacag aatgttccat cttcataaat ttcaaaggca
     4861 cgttccggtt ttctctgctg aaagctccag gaaaggctct cgccttccgg cagagtataa
     4921 tccacaagag aaaatgttcc ggctatgttt gccattccaa cagcatccgg gcttacttcc
     4981 agtcttgcat tctccagaac ggtcggttcg gtttcgttat ttgcctgaaa tgcaatatcg
     5041 tatttgtttt cgcctgtctc cggccattca taccgtcccg gtgtataatc ctgattttcg
     5101 ccaaagtatg gataaccgtc gcttccctgt ccccaggaag ttccgttttc gcctttattg
     5161 gcatttaact tttctaccag atccagcgtt ttcatgtcct cttcggacat tgcaccggaa
     5221 ccgctgccca gtgtatccag ccagtagcag ttctcaacga tcgccgcacc gctgttgttg
     5281 ctgctgctgt tgcaaagtgc tccttttccg gtatctccca ctgcgataca gtttgcgatc
     5341 acaccgtcgc ggtcatcgga agcacttgct cccatatagg ctccggtctt cccgatcaat
     5401 cctgccacgt ttgctccgct gatctcactt ttacagttca aaatggaagc accataggcg
     5461 gtcgttccaa acggtccggt cacattactg ctgcttccgg tcatcgttcc ttttaccgtc
     5521 agattctgga tcacagcacc tgctgacaga tttgaaaaca gcgagcctgc gttttcaaac
     5581 ataatggtat gtccctgtcc gtccagaacc ccggcaaacg gagaatcatt catataatca
     5641 ctgtttaccg tgatatcctg tgtcaggacg aaatatttgc cttttgcatt gtccatcttg
     5701 gaaaaatctg cctgggaaga gatggagatc acattctccg gcagtgccat cggttttgtc
     5761 gtgggtctgt ttttcagtgc tgcgatcgca tcggtgatcg cctttgctgc cgctgtgatc
     5821 tgctcctgtt tcacctcttc gcctgctttt aaagcatttc cggctgctac tgcatcagaa
     5881 agtgtcttcc agctctcttc cgtatagttt tcctcttcat agccttctgc ctgcttcaat
     5941 gcattttcaa gcggtgtcca gctgatttcg gccgctccct ttaccagaac cggaaatccg
     6001 ccatcttcgg agactgcaaa gatatagccg gtatcgacga tacctgtatt taacagagca
     6061 acggtgtctg ctgtttttaa ttcatcagca gatttggatg cacaatttgt aacctgtgct
     6121 ttattatctc catactgctt tccaatagct gatgttgctt tagaagtata atagctgtta
     6181 ttaatgacag aattggctga tttattatac gctgcaattc catccagcat aagagaagaa
     6241 cttgtaccgg cataataaca atttaaaata cttccgccat caagaacccc aacaattcct
     6301 ccaacttctt caaacatttc ttcatcgttg atcgttacag tggaataact gttctgaatc
     6361 gttccggtca gattatctgt aatacttccc ttgtcatccg actctacatc aacagatcct
     6421 gcaactccca ggttctggat cgtaccggat acgttttccg ccagtgcttt cccggaaagg
     6481 gtgatcacat gtccctgtcc gtccagagtt ccggcaagat ctgtgatctg ctgtccgtct
     6541 gccagcttaa tatccgccgt cagtgtatag gtttcacctg ccggaatttt ggtcggaaat
     6601 tcttctgcag ttgtgatttc attgctgctt tttgtcgtac tcgctgcatt tacgctcagg
     6661 cttgctgtat ttgccggaag aatgccggta aacagcaatg ccagagcaag catccatgca
     6721 aggatacgct gtgtgactgc tttgtgcttc atcgcttttc tcctcttctt tgctgatctt
     6781 ataatcgagt aggaggcggt gactaaccgc cgtcctctca caccaccgtg cgtacc
//
LOCUS       DAWDWH010000101         6667 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5156_length_6667_cov_16.200544, whole genome shotgun sequence.
ACCESSION   DAWDWH010000101 DAWDWH010000000
VERSION     DAWDWH010000101.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6667)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5156_length_6667_cov_16.200544"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1510 a         1720 c         1352 g         2085 t
ORIGIN      
        1 tcctttctac gctccgcttc ggtcggtgct aaacatgtgc cactggcaca tagccccctg
       61 gcatcttatt attctatttt aacacaaaca acttcagtaa aaaagcaaaa aaattataac
      121 atatgtaaaa acagtccgct tcttaatttc ggctcaaacc atgtggattt cggcggcatc
      181 agcatgccgg catcggctac ggcaaacagt tctgccatgg aagtcggata catggaaaaa
      241 gccaccgcat ctttctgctg cgtctctttt acatgatcga ccatacgctc cagctcggaa
      301 agtccgcgga tcccgcccac atacctgatg cgttttgatg ttttcggatc ctggatgtcc
      361 agatatggct gcaaaaattc tctttgcaga taagcgacat ccagaccttc caccggatcg
      421 tcttttgtaa gatctgcctt tgccgtaaga tgataccact gaccgctaag atacatgcca
      481 aaagtcccct ttttctgcgg atgatatgcc gcatttaccg gttgtacatc aaaaattttc
      541 gctgcgtttt ccagaaagcc ttctcttgtc tgtccgttca gatccaggac gacacgattg
      601 taatccataa cgtaaagttc ctcatccggg aataataccg acagaaaata attgaattct
      661 tcgctgccgt cataatccgg atgctgtttt ctgcgaagct gccctacttt gactgccgat
      721 gctgccctgt gatgtccgtc tgcgatataa attttatcta ttttcgcaaa tgtatctgca
      781 attattgcaa tatcctcttt tgcatccact ctccagcctt tatgtccgat gccatcttcc
      841 gagacaaacg caaacatcgg tttctgctgt tttatttttg ctgtcacctc tgcgatcgtc
      901 tgatctttgc ggtaggcaag aaagatcggt ccggtctgtg cattgcaggt atccacgtgt
      961 cggatacggt cctgctcttt ttctgccctt gtattttcat gctttttgat cacaccgttt
     1021 aaataatcgt cgatcgatgc acatgccaca agaccggtct gtgaccttcc atccatcgtc
     1081 agttcataca gatagtaaca tttctgctga tcctgcacaa aggagcctct tttgatccat
     1141 tcctctaaca tatcatgtgc tttctgatag acttccggtg catacatatc ctgatctttt
     1201 ggaaactggg tctccgggcg gtcgatcgcc agaaagcttt ccggtgcttt ttttacctgt
     1261 tctcttgcct cgtcacggct gtagacatca taaggaagag ctgcgagttt agatgcaagt
     1321 ccttctgccg gatgtacagc acaaaatgtt ctgatctctg ccataatcat tcctccatct
     1381 ctgcacggtc cgggcgtttc ggtttatgag ccgcctcttc cgtactgaaa aaggctgaag
     1441 cagaaaactg caacagcctt ttgtcctctt gtttttctta ttcttttgtt ttttatttga
     1501 tcactctgac tctcagcaca ccttcgactg cacggatctt atccagtact tcttcggatg
     1561 ccacgctgtc cagatcaaac agagagtatg cataatcgcc tttgctcttg tttaccatgt
     1621 ttgcgatgtt gatatcagat gttgcaaaaa tgtcggtaaa ctgtcccagc atgttcttta
     1681 cgttgcggtg gttgatcgca atacggtttg catgtgtgca ctcgcccaga ctgcagttcg
     1741 gatagtttac agaattgatg atatttcctt tttccaggta ttccatcagt tcctgaactg
     1801 ccatctttgc acagttgtct tccgattctg cggtggatgc acccagatga gggatgatga
     1861 tcgcattttc cacacctgcg gttgtggtat tcgggaaatc gcagacatat ttctttactt
     1921 ttccttcttt gagggctttt acaacagcct cttcatctac cagcatatca cgtgcaaagt
     1981 tcaggatcac gacattttct ttcatcagat cgatcgcatc ctgattgatc atctttctgg
     2041 tagaatccag cagcggtaca tggatcgtga tataatcgca agtacggtaa atatcgtcca
     2101 catctttgat gtgttttacg cctttggaaa gtttccatgc cgcatcaaca gaaatgtacg
     2161 gatcataacc atatacatcc atacccagac attctgccac gtttgccacc tggataccga
     2221 tcgcaccaag tccgatgata ccaagctttt tgcccatcag ttcgcatcct gcaaatttct
     2281 tcttttcttt ttctgccttt tttgcgatat cttcgcttcc tgcttcggat tttgcccatt
     2341 ccacaccgcc gatgatatca cgggaagcca gcagcatgcc tgcgatcacc agctctttta
     2401 cgccgtttgc attggctccc ggtgtgttaa atacaacgat acctttttct gcacatttat
     2461 ccagcggaat gttgttgaca ccggcaccgg cacgtgcaac tgcatacaga ccatccggca
     2521 gctccagatc atgcatggca gcacttctta ccagcactgc gtctgcatct tccatttttt
     2581 ctgttttttc gtaaccttct gtgaactgat ccagtcctac agaagcgatc gggtttaagc
     2641 atgtatattt cattttacag attctcctct tcaaatttct tcataaatgc taccagtgct
     2701 tccacacctt catatggcat tgcattgtag atacttgcac gcataccgcc aacggtacga
     2761 tgacctttca ggttttcaag tccggcttct tttgcttctt ttacaaattt tgcatccagg
     2821 tctttgtcac ctgtgacaaa cggaacgttc atcagagagc gggaatcttt ttctactgtt
     2881 cctttgaaca gcttgctgct gtcaagataa tcgtagagca gttttgcttt cttttcgttg
     2941 cgttctttca tgacttccag accgcccatc tttttcagcc atttgaatac cttgccgcac
     3001 atataaatgc agtagcagtt cggtgtgttg tacagagaac ctgcatcagc atgtgtctta
     3061 aatttcagca tggtcggtgt accttccagt acctcatctg tgatcagatc ttcgcggata
     3121 atggagatca ccatgcctgc cggtccgata ttcttctgaa cgccgccgta aatgatgccg
     3181 tattttgtga catcgaccgg ctctgacagg aagcaggaag aaacgtctgc taccagtgtc
     3241 ttgccttttg tattcggcag tgttttgaat tttgtaccat aaatggtgtt gttctcacaa
     3301 atatatacat agtctgcatc cgggctgacc ggcagatcgg agcaatccgg aatataggag
     3361 aatgtcttat cttcagaagt tgcgatcttg tttacttttc cgtatttctg tgcctcctga
     3421 tatgcttttt ttgcccactg tcctgttacg atataatctg caactttgtt tttcatcaga
     3481 ttcataggaa tcatggcaaa ctgctgtgaa gcaccgccct gcaggaacag cactttatag
     3541 ttatccggaa tatgcatcag atcacgcaga tcctgctctg cttctttgat aatatcgtca
     3601 aagacctgtg agcggtgact catttccata acggacatac cacagccctt gtaatccagc
     3661 atttctgctg ctgcttcttt taatacttct tccggtaata cggccggacc tgctgaaaaa
     3721 ttgtacactc tgctcacttt catatcctcc ttgacttctc actttgttgt gtgatgagtt
     3781 ttatagcaag tgctgatgcc tgttttcaaa actccgccag atatcagcac ttattttcta
     3841 cactttacta tagtactttg ttaaaaatta gtcaagcctt tttccaagtt ctggaaaaag
     3901 gcttacagaa ccgaatttat gcttctttat tcagatcgtc ctggtcaaat acatcttcga
     3961 tcggtgcacc tgccggaacc atcggaaaga ctttgtcgtc gcagtcgatc acacagtcga
     4021 tcacaaccgg tttgccaagt gcgatcgctt tttcaacagc cggtgcgact tcttcttttt
     4081 tcgttacacg gatgccttct gcaccaagtg cttctgccac tttcacaaaa tccacaccgt
     4141 cattcagaat ggtctgtgaa taacgttttc cgtagaacag tgtctgccac tggcgaacca
     4201 taccgagcac atggttattg atgacaacct gaatgatcgg gatgttgtat cgggctgcgg
     4261 ttgcaatctc attcatgttc atacggaaac atccgtctcc ggcgatattc agtaccactt
     4321 tatccggtct gcccactttg gctccgattg cagctccgac accataaccc atggtgccaa
     4381 gtccgccgga tgacaggaag gtacgcggat ctttgtaacg ataatactgt gcagcccaca
     4441 tctgatgctg tccgacatcg gttgtgatga tggcatttcc ttcggttact ttatagatct
     4501 gctcgatcac atacggaccg gtcagacctt cttcattgta tttcagagga tgctcttcgg
     4561 agattgtctc aatctctttg atccattctt tatgctcatg tttttcaatt ctctgattga
     4621 gacgtgtcag cacttctttg agatctccga taatactgca gtctacctga atgtttttgt
     4681 tgatctctgc cggatcgaca tcaatctgga gaattttggc attttttgca aaacggctgg
     4741 catttccgat gacacggtca ctgaaccggg aaccgaccgt gatcagcaga tcacattttg
     4801 ttacacctaa gttggatgct tttgttccgt gcataccgag catacccatg taaagctcat
     4861 cggtgccgtc aaaagcacct tttcccatca ggctgtctgc aaccggtgcc tctattttct
     4921 ttacaaactc catcagttct ttggaagccc cggatgtcac ggcaccgccg ccgacaaaaa
     4981 caaatggctt ttcactctca ttgatcagct ctgttgcctg tgccagatct ttttctttga
     5041 tatactgtgt gctgcgttct accgggaacg gctcttcttt tacatactcg gtctctgctg
     5101 cggtcacatc ttttgtgata tcaaccagaa caggaccggg tctgccttcc tgtgcaatgc
     5161 ggaacgcttc acggatggta tctgccagtt ttgtgatgtc ttttacaata tagttatgct
     5221 ttgtgatcgg catcgttaca cctgtgatgt cgatctcctg gaaactgtct cttccaagca
     5281 gcggaacggc tacgttcgct gtgatggcaa ccactggaat ggaatccata tatgccgttg
     5341 caataccggt taccagattc gtggctccag gaccggaagt tgccatacat acgccgactt
     5401 ttccggttgc tcttgcatat ccgtctgccg catgagcagc tccctgctca tgggatgtta
     5461 agatatgatt gatctctgaa ccatgtttat ataatgcatc gtatacattt aaaatcgtgc
     5521 cgcccggata accgaaaaca gtgtctacgc cctgctcttt caggcactcg attacgattt
     5581 ctgaaccatt taacttcatc ctgctgtcct cacctttact tttcttactg ttgttgctta
     5641 gcttcaattc ttgtttcaga attatttttc tgaaacaaga atagctcctc tgttaccgga
     5701 agttaccata gaagcgtaac gtgccagata gccggtcttt accttcggct ctctcggtgt
     5761 ccagttggct tttcttcttg ccagctcttc atcagaaaca gccagttcca gtctgttttc
     5821 cggaatgttg atcttgatga tatcgccttc ttctaccagt gcgatcggac cgccgactgc
     5881 tgcttccggt gatacgtgac cgatggaagc tcctctggac gcaccgctga aacggccgtc
     5941 tgtgatcagg gcaactgtgg aaccaagtcc catacctgcg atctcagaag tcggattcag
     6001 catttctctc ataccagggc cgccttttgg tccttcgtaa cggatcacga caacatcgcc
     6061 ttctttgatc tttccagaga gaatagcctc acatgcatct tcctcacatt caaagactct
     6121 tgccggacct tcatgtacca tcatttcagg cacaacggca gaacgtttta caacacttcc
     6181 gtccggtgcc agatttccgg tcaggacagc cagtccgcct gtcttgctgt acggattctc
     6241 taccgggcgg atgacttccg gatttaagtt tacgcagtct ttgatattct cgcctacggt
     6301 ctttccggta acggtcatgc agtcggtgtg cagcagacca agtttattta attcattcat
     6361 aacggcataa acaccgccgg cttcattcag atcctccatg taggtcgggc ctgccggtgc
     6421 caggtggcag aggttcggtg tctttgcact gatctcattt gcaaatgtga tatcgaaatc
     6481 catgccgatt tcatgtgcaa tcgccggtaa atgcaacata ctgtttgtag aacatcccag
     6541 tgccatatcc acggtcagtg catttaaaat tgcatcctct gtcataatgt cgcgtggacg
     6601 gatattctga cggtacattt ccataacctg catacctgca tgttttgcaa gacggatacg
     6661 ctcggaa
//
LOCUS       DAWDWH010000102         6628 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5181_length_6628_cov_8.070896, whole genome shotgun sequence.
ACCESSION   DAWDWH010000102 DAWDWH010000000
VERSION     DAWDWH010000102.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6628)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5181_length_6628_cov_8.070896"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2989..2998
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1487 a         1684 c         1366 g         2081 t
ORIGIN      
        1 ctgcttttgc gttcggtcct acaacttccg gatcagctgc aaattttact tcctgaccaa
       61 gcagttcaga gagacgtact gctaccggtg ccagagagag ttccggtttc ggttctccct
      121 ttggtttgcc cagatgagag caaaggatta cttttccgcc gtccgcgatc agttttttga
      181 tagttggcag agctgctacg agacggtttt cgtctgtgat cttgccatct ttcagaggta
      241 cgttaaagtc acagcgaacc aggactcttt tgccttttac gttgatatca tcaaccgact
      301 ttttattaag cattcttatt tcctccttgt atcatacata cggattttct aatgtgcatc
      361 cttgcggagc cgcttattta gccgcagagt cttctccgac taagataaaa agggcccggt
      421 ccaaaagacc ggacccagat aggtcttctt tgtctgattt aacagattat gctaattcag
      481 agaagtattt gattgttctt accatctggc ttgtgtaaga gttttcgttg tcataccaag
      541 aaacaacctg tacttctgtt gtaccgttgt ccagaggcag aaccattgtc tgagtagcat
      601 caaacagaga accatatctc ataccaacga tatcgctgga tacgatttcg tcttcgttgt
      661 atccgtaaga ttctgtagca gatgctttca tcttagcgtt gatttcgtct acagttactg
      721 ttccttcaac aactgctgtc aggattgtag tagaacctgt aggggtcggt acacgctgtg
      781 cagaaccgat cagtttgccg ttcagttccg gaataaccag accgatagct tttgcagcac
      841 ctgtgctgtt cggaacgatg tttactgctg ctgcacggga tcttctcaga tcgccttttc
      901 tctgaggtcc gtccagagtc atctgatcac ctgtgtaagc atggattgtt accatgatac
      961 cggatttgat cggagccagt tcgttcagag ctttagccat tggtgccaga cagtttgttg
     1021 tacaagaagc tgcagaaatg atctgatcct cagctttcag tgtctcatgg tttacattgt
     1081 aaacgatagt cggaaggtca ttgcctgctg gtgcagagat aacaacttta cgagcacctg
     1141 cattgatatg agcctgagcc ttttctttgg atgtgtagaa tccagagcac tccagaacta
     1201 cgtcaacacc cagctcgccc catgggcagt tgtttgcatc cgggaatgcg tagatcttga
     1261 tctcttttcc gtcaactgtg atggaatctt cacctgctga tactgtgtct gccagtgcat
     1321 atttaccctg tgatgaatca tattttaaca ggtgtgccag catttttggg gaagtcagat
     1381 cgttgatagc tactacttca tatccttctg cgccaaacat ctgtctgaat gcaagacgtc
     1441 cgatacgtcc aaaaccatta attgctactt ttactgccat gattaatttc ctcctattat
     1501 gttaaatagt atgattgcgg tctttattgt taaagattcc acaactttct tctattctac
     1561 ctaatttctg taaaaagttc aagtataatt tcaacgaatt tctctggaca tttgtgtttt
     1621 attctcataa acttgtaaca gagagaaaaa tcaggtgttt tttcctgaat tttgtcgtat
     1681 tttgaaagga gtttttattt tatgagatcc gattttcatc ttcattcctc tttttccgcc
     1741 gattccgacg ccccgatgcg ggatatggtc gaaaagggta tctctcttgg actgtctgcc
     1801 atgtgtttca cagagcacat ggactatgac tatattgacg aagggatggt cttcgaagct
     1861 gatattcctt cttattgcaa gacgcttacc tccctcaaag aagagtatga gcacaagatt
     1921 gaattattat tcggcctcga actcggtctg cagccttatc tgaaggagcg ctgcgaatct
     1981 cttgtaaaga attgggattt tgattttgtg attggatcat cccatgtggt aaacggacag
     2041 gatccctact atcctgcttt ttttgaagga cgcaccgaaa aagagtctta tatttcttat
     2101 tttgaatcga tcattgagaa tctgcatacc atgtcctgct ttgatgtcta cggccatatt
     2161 gattatgtcg tccgctacgg tccaaataaa aatcagttct attcttataa agaatacagt
     2221 gactggctgg atgagatcat ccgtcttctg atcgataaag gacttggcat ggagatcaat
     2281 accggaggct atgcagccgg acttggacac ccgaacccgc acgaagatat cctgaaacgt
     2341 taccgcgaac ttggcggcga gatcatcacc atcggatcag atgcacatgc cccagaacgt
     2401 attgcggctg attttgaccg tgcctgttcg atattaaaaa aatgcggctt ccgttatcat
     2461 gcgatattcc gcaaacgaaa gccgactttt gaacttatat aatcgtaccg ccgcccagca
     2521 cgtatccgtc ttcatagagc accactgcct gtcccggcgt gatcgcatgc tgtggctctt
     2581 caaaggtaca ggtaatctca tcttctccgg tccgctttac atggcagtat gcccctttgt
     2641 gattgtagcg gatctttgca aatacatgat cttcttcctg aatatcttcc cttgccatga
     2701 aatttaaatg gttggcacga agaaccgtct gacaggcctg ggcacggttt ccgatcacca
     2761 cttcgtttgt ctgtggtcgg atctctgtga caaaagccct ctctccaagt gaaattccca
     2821 gaccttttcg ctgtccgacc gtataatgga tgatcccttt gtgtcttccg aggattttcc
     2881 cgtccggtgt cacaaaattg ccttccggaa cggtctttcc ggtatattct tcgataaagg
     2941 aagcgtaatc gccattcggt acgaaacaga tatcctggct gtccgcctnn nnnnnnnnac
     3001 ttgacatagc ggttgcaggc gatacatgga ttcggcgtct gtccattcag ataagaatcc
     3061 gcaaaataat ccatgacatt tttccgaaaa tcgtctttaa aattcatcac ataatatggt
     3121 ataccgatcg tctctgccac tcttcttgca tcatccactg cacttaaccc gcagcatcca
     3181 ccgttttcct gctggacttc ctcggcttca tcctgccaga tctgcatcgt cacaccgact
     3241 acatcatagc cctgctgttt cagcagcatt gctgccacag atgaatcgac tccgcccgac
     3301 attccaacca ctactttttt actcataatc ttttccgcct tactcctttg atcataaaat
     3361 cggcatcctg tatgctgatc ttagtactcc tcttcctctt cttcttcgcc ttcatggata
     3421 tcagacttcg gcttttgcag tccttcgatc ttgatgtggt gtttctctgc gtaatcccac
     3481 agtgctgcat ggatggcttc ttctgcaagc agagaacagt gtacttttac aggaggcagt
     3541 ccgtccagag cttccataac ggctttgttg gtaacctgca ttgcttcctg gatatttttg
     3601 ccttttacca gttcggttgc catgctgctg gttgcaacgg ctgctccgca tccaaaggtt
     3661 ttaaatttta catcgcggat gattccgtca tcgtcaatgt ccagataaat acgcatgata
     3721 tcgccgcatt ttgcattgcc gacggttccg acaccacttg cattttcgat ttcgcctaca
     3781 ttacgcggat gattaaaatg atccataact ttttcgctgt acataaaaat ttcctccaat
     3841 tttctgtttt gtttttctat ctttattctg gcttattttc tgactatttc tgtgctctgt
     3901 acaatctgat cctgtctttt actgccggat tatttctgat tctttttcac gaaatcttcg
     3961 tagagtggag acatgcttcg cagtctttcg ataatctgtt tgatacagtc tacaacgaaa
     4021 tcaatgtcct ccatggtagt ctctgcggac agtgtcagac gcagggaacc gtgtgcgatc
     4081 tcgtgtggca ggccgatcgc cagcagtaca tgggacggat ccagagaacc ggatgtacag
     4141 gcagatccgc tggaagcaca gataccgttg ttgtccagca ggatcagaag ggactcacct
     4201 tctacaaact gaaaactgaa gtttgcattg ttcggcagac ggttggttct gtgtccgttc
     4261 aatctggtat aaggaacttc cgccaaaaca cggtcgatca gatgatctct cagttcgatc
     4321 tcttttgcgg tacgctcttt catggtcttt gctgcgattt ctgctgcctt gccgtacccc
     4381 acaatacccg gaacattttc cgtaccggca cgacgtttac gctcctgtgc tccgccgtgt
     4441 acgaaagaac ggatcttaac gcctttgcgg atgtaaagga aaccgattcc cttcggaccg
     4501 ttcagtttgt gtccgctgga acttaacata tcaatgtgaa gctcgtcaac attgatcggc
     4561 agctgaccgt atgcctgaac cgcatcggta tggaacagaa cgccgtgttt ctttgcgatc
     4621 tcaccgatct ctttgatcgg ctgaatggtt ccgatctcat tgtttgcaaa cataacggag
     4681 atcagaatcg tatccggacg gattgctttt tccagttcat ccacttttac gacaccgttt
     4741 tcatccacac cgatgtaagt gatctcaaaa ccacgttttt ccagatattc tgcggtatgc
     4801 aggatcgcat gatgctcgat cttggtcgtg atgatgtgtt ttcctttgct ctgataagct
     4861 tctgccgttg ctttcagtgc ccagttatct gcctcggaac caccggctgt gaaataaatc
     4921 tcattttcct gtgctcccag agtctctgcg atcgttcttc ttgcatttga aactgctttt
     4981 ttgctctcgc ttgcaaactc gtaaacactg gacgggtttc cgtagtattc tgtaaagtac
     5041 ggcagcatag ctgctactac ctcttctgaa gtctttgttg ttgctgcatt gtccaaatag
     5101 atcaatttct tcatatttac ctcctgatgc cgcctgcggc acaatctcaa cactttctct
     5161 ttgtgtctgc tttatcctgc gaatttatat actgaccgga ctgctccatc tctcgtttcc
     5221 gctcctggct tttctttgct tctgccatca gctcctcaag cgtaatctca tctactgctt
     5281 tcgcaatgct gtcgttgatc ctctgccaga ccagtttcgt cacgcagaaa tcagaacttt
     5341 cacatccgcc ctcaggcttc agtccctgac agtccaccgc tccaagctct ccttccagag
     5401 cccgcagtat atctccgact gaaatctcgg ccgcaggttt cgccagacgg taaccgccct
     5461 gggcacctct cgtgctcttg atcaaccctg ctttttttaa ttttcccgca agctgttcca
     5521 gatagctctc agatatgttc tcacgctgtg caatgcagct gatggatacg gattctttct
     5581 cactgtatac cgccaggtca accatcgccc gcagtccata tcttcctttt gtagataatt
     5641 tcatgctgcc ctcccagatt gtttcatgcc ggaatcagaa attcttctga tcgcggcacc
     5701 attttctgga ttaattccga gtgtttcgct gggatttgct tcgaatatag caccgcattt
     5761 ccgttctgtc aagtacttcc tccgaatata tataagaaaa aatggaattt tgtgcttatg
     5821 atccagaaac atccgctcct gaaaagcacc gccatcctga cctgtgccgg tatggtcagc
     5881 cgcatcatcg gtttcttcta tagaatattc ttatccaaga caatcggtgc ggaaggaatc
     5941 ggcatctatc ttcttctttt tccgatccag accctgctgt tttccctgac ctcatccgga
     6001 atccagactg ccatttcccg ctttgtctct gcaaggcttg catcgaacga caaaaaagga
     6061 gccagagaca tccttctttg cggtatgatg ctctctgtcc tgttttccgg gattgcttct
     6121 ttttttcttt atcagaatgc gaattttgtt gccgtctctc tgctgcatga ggcacgctgc
     6181 agctcccttc tgaaaatttt aagctttgca ctgccttttg ccggtgtgca tgcctgcatc
     6241 atcggatatt ataccggact gaaaaaagcc gccgtccctg ccatctccca gctggtcgaa
     6301 cagatcgccc gcgttgcctc ttcgttcctg ttctggcaga tcatggtgga aaaaggaatc
     6361 caggtaaccc ctgtacttgc catgtatgct atgctgacct cggagatcat cgtggttctg
     6421 tataccggaa cccggtttct gctccaccat ccgtcttctt ttgaaaaagc atgtccgttt
     6481 tccgcacaca gtatccggga aatccttact ttttcgctgc ccttaagtac aaaccgtgtc
     6541 tgtttaagtc ttctgcaaag tgctgaatcc atctgtatcc cgctgcgact gcaggctttt
     6601 ggccttgcaa gatctgcagc actttctt
//
LOCUS       DAWDWH010000103         6624 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5186_length_6624_cov_13.249049, whole genome shotgun sequence.
ACCESSION   DAWDWH010000103 DAWDWH010000000
VERSION     DAWDWH010000103.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6624)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5186_length_6624_cov_13.249049"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1930 a         1394 c         1597 g         1703 t
ORIGIN      
        1 aatgaagctg caaagtgcag gggagaaaat ttgcagatcc cgggtgtgac agggtggaga
       61 gatcttcgta ttgcagccga tgttttttct tatgaaaagc aaataattcc gcaaaagaag
      121 tgtacaaaat ggtttgatta tgataccata aaccatagac tggagattcg tacgcgtcag
      181 gcgggcgata ctctcagtgt gcatgcagaa ggcgggacaa agcctctgaa aaggtatatg
      241 atcgatgaga agatcctgcg agagagacgg gatgagatac cgctcattgc ggacggctcc
      301 catattcttt gggtggtcgg ataccggatc agtgaggcat acaaggtaac agaacgtaca
      361 aaaaagattc tgcaaataca aatagataag gagaactaaa atggaagata agattcgtgt
      421 actgttgaca gaagaagaag taaataaaaa aatcaaaaaa atcggtgagc aggtcagcaa
      481 agattatgcc ggcaagtccg ttcatatgat ctgcgtgctc aaaggcggcg tatttttcac
      541 ctgtgaactg gcaaagagaa tcaccgttcc ggtatctctc gattttatgt ccgtgagcag
      601 ctacggttcg gagacaaaat caagtggtat cgtaaagatc atcaaagacc tggatgagcc
      661 gctggaagga aaagaggtcc tgatcgtaga agatatcatc gattccggac gtaccttaag
      721 ttatctgatc gagatcctga agaaaagaaa cccggcaagc atccgtctgt gcacgcttct
      781 ggacaaaccg gaacgcagag tgacagatgt aaaggtagat tatgttggtt tcaacattcc
      841 ggatgaattt gttgtaggat atggtctgga ttatgcccag aaataccgca accttccgtt
      901 catcggagtg gtagagcagt agaaagggga acccgaattg aagaatgttc taaaagggag
      961 tatcttctat gtcattattg cgatcattgt ggttctgggg atcaattata tccggggcaa
     1021 cctcgtagag aatacaggag tcacctattc acagttccag aaatatgtcg cacagaataa
     1081 ggtcagcggc gtagaggtca gacagaattc tcagggagtg acaggagagc ttacggttca
     1141 tctgaaagat ggggagtaca gaacgctcga tgtttcggat gtgacaaaag cagaagatct
     1201 tcttgatcag aaaaatattt cctataaggt tacagttgaa aaagagagcg tttttatgac
     1261 aacggtactg ccgatgcttc tgatctgcgt gatctttatg gtatttttta cgatgggcag
     1321 ccgtcaggcg acgggaggca atgcccggat gatgaatttc ggcaaaagcc gtgcaaccct
     1381 gaccatgccg gacaacagca aagttacgtt taaagatgtt gccggtcttc aggaagaaaa
     1441 agaagatctg caggagattg tcgatttcct gaaagaaccg aaaaaatatc tccaggtcgg
     1501 agcacgtatc ccgaaaggtg tgctgcttgt cggccctccg ggaaccggta agaccctgct
     1561 ggcgaaagcc gtggcaggag aagcgggtgt tccgttcttc agcatttccg gttccgattt
     1621 cgtggaaatg tttgtcggtg tcggtgcatc ccgtgtgcgt gatatgttta ccgaggcgaa
     1681 acttcatgca ccgtgtatca tctttatcga tgagatcgat gcagttgcca gaagacgagg
     1741 agccggcgta ggcggcggtc acgatgaacg tgagcagacg ctgaaccaga tgctcgttga
     1801 gatggatggt ttcggataca acgaaggcat catcgtgatg gcggcgacaa accgtgtgga
     1861 catcctcgat ccggcgatct taagaccggg acgtttcgac cgcaagatcg gcgtcggacg
     1921 accggatgta cgcggaagag aagagatcct ggcagtccat atcaaaaaca agccgcttgg
     1981 agaggacgta gatctcaaag agattgccag aacatccgca ggatttaccg gggcagatct
     2041 ggaaaacctg atgaatgagg cggcaatcta tgcggcaaag gcaaaacgtg cctatatcat
     2101 gcaggaagat atccgccgtg ccttcatcaa agtcggcatc ggcgaggaga aaaagagtcg
     2161 gatcatcagc gagaaagaaa agaagatcac cgcttaccac gaggcaggtc atgcgatcct
     2221 gttccatgtg ctgccggata tgggaccggt ccatacgatc tccgtgatcc cgaccggaat
     2281 gggggcagcc ggttatacga tgccgcttcc gggtgaagat gagatgttca acagcaaaaa
     2341 gaaaatgctg cagaatatca tcgtggactt tggcggacgc gttgcagaag aactgatctt
     2401 tggcgatgtg acgaccggag catcccagga tattaaacag gcaacgcaga tggcaagagc
     2461 catggtcaca cagtacggta tgtcggacaa agtcggtctg atccattatg gaagcgatga
     2521 cgatgaagtc tttatcggac gcgacctggc acatacaaag ggttacgcag accagacagc
     2581 tgcactgatc gacggtgaag taaaaaacat cattgatgag tcttatgcaa aagcaaaaga
     2641 acttcttcag gagcatatgg atgttttaca tcgctgtgca cagcttctga tcgagaaaga
     2701 aaaaattggc agggaagagt ttgaaagcct ttttggtgaa aatgcataaa aaataatttc
     2761 agattttgtg aagattatgc acacttaaat ttgtacctgt ggtatattat tactcgtaaa
     2821 acccccaata cattatatag tttttgctac accccataag aagaaatacc ttctccaaag
     2881 aaagacagtt catatgaact gtctttttcc cttttctgga ttatttcgtt gtgaatttga
     2941 aaaatatggt gtaagatagt aataattcta cagtttgaat gcaggttatg aacgtcttgc
     3001 aggcgtttcc tttaaaatga ccgcaaaaga gccgtaagaa cgtgatccgg gtgtgtgaag
     3061 acgctcctgg tcttcttagg aggaaaatat gaacttagca aatatagaat atcaggataa
     3121 gcttcgtcag tatgtgctgc agatgcgtcc tgtgtttaaa ccgaatgcac ttttcagcga
     3181 tgagacggca aattatgtaa caccgatgga acctgatccg ggcgacattg ttacgatacg
     3241 tttccgaacc tggaaagata atgttgataa agtatatctg gtaagcaatg acaggcatct
     3301 tgctatggaa tacgaaaaat cagaaggaga ttttgattat ttcaaaatcc gtctggtcat
     3361 gcagggagaa tctgtccgct atttttttga gatacatgct ggcaagatcc gatgtttcta
     3421 caacaagatc ggtgtatcca gggatcgcct cgatgtctat gattttacaa tcgcaccggg
     3481 tttccacacg ccggaatggg caaaaggtgc tgttatgtac cagatctttg tggatcgttt
     3541 ttcaagcgga gacacgaaca ataccgtcga agactgcgag tactattaca tcggcgacta
     3601 ctcccggaag gtgactgact ggcggaagaa tccatccttt atgggcgtgc gtgaatttta
     3661 cggcggtgac cttcaaggtg tgatgaacaa gctggattat ctgcaggatc ttggcgtgga
     3721 tgttatttat ttcaacccga tctttgtctc accgtccaac cataaatacg atatccagga
     3781 ttatgattac attgatccgc actatggaaa gatcgtaagc gacggtggcg agactctgaa
     3841 ggacggggat acgatcaatg cccatgcttc taaatacatc aaacgagtaa ccgacaaagc
     3901 aaacctggag tccagcaaca ctttttttgt tcagctggtc gaagaaattc accgcagggg
     3961 catgaaagtg atactggacg gtgtgttcaa ccactgcggt tcctttaata aatggatgga
     4021 ccgtgagcag atttatgaga atcaggaagg gtatgaaccg ggagcttatg tttccgcaga
     4081 cagtccttat cgcagctttt tcaaattcaa taacgaccag gcatggccgt acaacccgaa
     4141 ttacgatggc tggtggggac atgataccct gccgaagctt tgctatgagc agtcaccgaa
     4201 gctgcacgat tatattttgg agatcggaaa gaaatgggtt tccccgccgt tcaatgtcga
     4261 tggctggaga ttggatgtgg ctgctgacct tggattttct aatgaatata accatcagtt
     4321 ctggaaagaa ttccgcaaag tcgtaaaaga ggccaatccg aatgcgatca ttctggcaga
     4381 acattacggc gatgccaagt cctggcttcg cggtgatgaa tgggataccg ttatgaatta
     4441 tgatgccttt atggagccac tgacctggtt ctttaccgga atggaaaagc acagcgatga
     4501 gttccgcggc gacctgctcg gcaatgaagc agcgtttacc ggagcaatgc gtcatcatat
     4561 ggcaagcttt ctggcaccgt cgcttcaggt ggcgatgaac gagctttcca accatgacca
     4621 ttcccgtttc ctgaccagaa cgaaccacaa agtagggcgt gtggcgaatt tgggatatga
     4681 ggcagcctcc caggatatca atgtggcggt catgcgtgag gccgttgtga tgcagatgac
     4741 ctggccggga gcaccgacga tctattacgg cgatgaggcc ggagtctgtg gatttactga
     4801 tccggacaac cgcaggacct atccgtgggg cgaggaaaat cacgacctga tcacattcca
     4861 caaagaaatg atctgtatcc acaaagcatg tcctgtgctg acacatggct ccctgaaatt
     4921 cctggagcag agacataacg tactttctta tggaagattc tcgcaggacg agcagatggt
     4981 tgtggcattt aacaacgatc tgaatgagca gacgatcacc ttgtctgtat ggcaggtaaa
     5041 tgtaccgcag cataactgta aaatggaacg cctgatgctg acgcatgcac agggctatac
     5101 gaccgagcag tactttacag aagtgcatgg cggaaaaatt gagctgacat tgccgccgct
     5161 gtccgggatt gtgctccgac acggggaata agaaaataaa aacagttgat aagataaaaa
     5221 acccggaagc ctttcttttg attaagataa aaggttcccg ggtttttctg ctgtcaggga
     5281 agaatgtggg ataagacgga aaatacggtt ttgggaaagc tggcggattt cagattaggt
     5341 atgacaggta ttatgagtaa tttaatgaat taaagcgata aagcgaaaaa ataaaaaata
     5401 aaagcgcgag acgggactcg aacccgcggc ctcgaccttg gcaaggtcgc gctccaccaa
     5461 ctgagccact cgcgcatatc atattgtctt ttgctgttcg cctcaagaca agtgatatca
     5521 taccgcagcc agtcccaaaa gtcaagcact ttttttattt ttttatattt tttacttttt
     5581 tactctaccg tacagctcca catgtggaac actcatcata tgaccctgac actaactctg
     5641 ttttcttagc acccattaaa atacgctcca aagaatagtt gctgcatccg gtatagaaat
     5701 cactttcccc cagatgaata atagcagcat gatcatccct tccaaaatct ttcttgcatt
     5761 ggttgcatac atagtgtctt tcatatactc cgctataaga tttaggaaaa atcggttttc
     5821 cataatcatt tctttcaagc tgatctaccg gtataaaatc ttccctgtat tcagcttcat
     5881 gatgtaccca gctatgctga tgattttccg ctcttccatt tgacacaatg gtagcatcta
     5941 ctgtacctgt ataatcaaat gctactgctc catttatgat ataccagatt cctgcatcat
     6001 tgcccgcaaa accagtatag ccaaaatcca cctgtccttt tgtgattttc caccagccgg
     6061 cttcattttg tgccaatcca ttataagaag cagcaactga tccaccgtca aaatagtacc
     6121 acttatttat atgatagtca tactggatac catatgcaga aaaatctacg cctccatctt
     6181 ttattttcca ataaccacta tcatttttta caattccatt ataatcatga tcgacattgc
     6241 catttacaac ataataccag tttccatcgg aaaactgctg cacacttcca tccggaacaa
     6301 ctatatccgg attagtattt tcactgtttt cctgatatgg ctgttgtgcc tctatccagc
     6361 ctttcgtagt gaaacgtatg gacacaccgc cttgattatt ttctacccat ctgtatgcgt
     6421 gatacttgat gatatagtca ggaagaaccc gtctgtactc actttctgta attttatttc
     6481 catataaaac aatatttccg cgcaaatcat ccatactatt gtaatagtat aaaaaatcct
     6541 caaaattaac tttaaaacta cagctgtcga acaatgtcct caatggttct atgttactga
     6601 tgttattatt ctgcagcttc agtt
//
LOCUS       DAWDWH010000104         6601 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5201_length_6601_cov_12.407119, whole genome shotgun sequence.
ACCESSION   DAWDWH010000104 DAWDWH010000000
VERSION     DAWDWH010000104.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5201_length_6601_cov_12.407119"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1289..1298
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4921..4930
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2255 a         1275 c         1668 g         1383 t
ORIGIN      
        1 ggaatcagaa atggaagaaa gaaaagatta ttataaaatc cttggaatag ataaaaacgc
       61 agatgaaaat accatcaaaa aagcataccg taaactggca aagaaatatc atccggatac
      121 caatgccgga aatgcacagg cagccgagaa gttcaaacag atcacagaag cctactcggt
      181 gctcagtgat ccggagaaaa agaaaatgta tgaccagttc ggcagtgccg attaccaggg
      241 cggtcaggga ccgtttggcg gagcaaatgg cggataccag gagttccatt ttgatggcgg
      301 tgacatggat gatatcctga aaaatatctt tggcggcgga ttctccggcc gcagtgccgg
      361 agcaggcggc ggaagcagat cttatcattt cggcggcgga tttggagacg gattctcagg
      421 cggaaacagt ggtttcggcg atggattttc cggtacaggc agtagctttg gcggcagggg
      481 cagtgacctt cacgccgata tcaccgtcag cttcgatgaa gcagcacttg gcggtgacaa
      541 ggtgatcaat ctgtcggatc cttctgcacc gggcaggaca cagtcgcttc aggtgcatat
      601 cccggcaggt attgatgacg gtcagagcat ccgtttaaga ggcaaaggtc agatgggcag
      661 aaacggtcag gcaggagatc tgcttctgaa agttaaaatc gcagagaaac cgggctttga
      721 acgaaaaggc atggatgttt acacgaccgt caatgttccg ttttccacgg cagtccttgg
      781 cggcgaagcg aaagtccata cgctctacgg cgatgtgatc tgcaaagtaa gggaaggcac
      841 acagtccgga agcaagattc gtctgcgagg caaaggtatt gtttccatga agaacccgca
      901 ggtacacggc gatcattatg tgaccgtcca ggtacaggtg ccgcggaaca ttactccgga
      961 agccagacgc aaactgcgtg agtttgagca ggcatgtggt atcagaaaca gctatgcagg
     1021 ataaatatga aaataaaaag tataaaagcg accgtaagtt ttacgatatg ctaatatcag
     1081 agcggataac ggagagattg taaagacata aggaaacctt gtaattgtat aaaaaaatag
     1141 aaagtgcagc atacaaaaag gaagaagagt atgctgcatt ttttatgtca ataggaaaaa
     1201 gataataaat ctgtatccgg gctgttaaat ttagatattt tgtggattta tcaggaaatg
     1261 atattgagat taactggaca aaatgacgnn nnnnnnnntt gagcaaagaa aaggaggaca
     1321 acatgaaaac aagaaaaaca ttttcagtca tgctggtgat ctgtatgctg ctgtcactgg
     1381 tgtttcccag tagtgggggg ggtgtcaaat gcagcctcag cttctgataa ggtaacagtt
     1441 attgatgtca cacagtatgg ggctgacccg accggagtaa aagacagtgc cgagggcata
     1501 caggcagcta tcgaggcagc aaaagaagtg aacggaccgg ttgtgttgga tttcccgaaa
     1561 ggggaatatc agatttaccc ggatcatgca cagaaacggg aactgtatat ttcaaatact
     1621 ttaagccgta ataacggtga ccgtggcacg tacaaaatga aaaatatcgg tattctgctg
     1681 gaaaatatgg aaaatgtgac actggaagga aatcagtcca gtctgatctt ccatggcaag
     1741 atgatgatgt tttccacgat cggatgtaaa aacatcagaa tccagaattt tgacaccgat
     1801 ttccaggtac cgtcggtagt cagtgtaaca gcagaaaaag tagaaggcaa tacagcgatt
     1861 ttatatgttc cggaatgtta taattattcc gtaagcggaa caaccgttac ctggagcagc
     1921 gatgtcagcc catacaccgg acaggcatac tggagttata cgaacaatgt cggacatgta
     1981 cagggatttg atatgacaac cgctaccctg actacttccg gcgataaatt tttcaacaac
     2041 gtacagagca tggaaaaact ggacggacat cgtctgaagg tgacttacaa ttctgcacca
     2101 tccttcaagg caggatatgg tcagcagatg agaaggacta cacgtgatca ttccgcagcc
     2161 tttttctggg agagtgatgg tgtgacgatg cagcatgtcg aactgcattt tctgcatgca
     2221 tttggtgtgg taggacagct ttccaaaaac attacactgg atgatgtaaa tttccgcaac
     2281 tatgaaggaa gcggaagaac cggcgttggt tctgctgact ttgtgcagat gtccggctgc
     2341 ggcggaacaa tccgcattgt gaacagcaca tttgaagatc cgcaggatga cccgatcaac
     2401 atccatggaa cattcttaca ggtaacagaa cgaatctctg ataataagtt caaagttcac
     2461 tatcagcacc acgaaacttc cggttttccg aactattatg tgggagatga agtagagttt
     2521 gtttctaagg gaacactgct tccaatcgac ggggcaaaag caaaagtaac cggagtggta
     2581 ggtccggacg gacatggcgg aaccatggtt gccggaaatg gtgacaaagc cgatgcttcc
     2641 aaaacggatc tggatacgat cattgtaaca ctggatacaa atatgccgtc ttccgtgtct
     2701 gcaaatgcgg cagctctgga aaatgttact tatacaccga gtgttgagat tgagaacaac
     2761 gtattccgtg agatgccggt acgtggtatt ctggtgacaa ccagaaaacc ggtcgtgatc
     2821 cgcaacaacg aatttgacaa cgtagccggt gcagccgttt acatttccga tgacgtaaac
     2881 agctggtacg agtcaggaca tgatgaggat gttttgatcg aaggcaatgt attcagacgc
     2941 tgtggtgtca accagagttc ttacagtgca tttatccagt ttgatccgac caacaacgga
     3001 gcagcggaac cggtacataa aaatgtaagg atcaaagaca atacattcta ttttacaaac
     3061 ggaaccagca atattattaa tgcaaagagc gtaaacggac tgacctttac cggaaacaaa
     3121 gttctgcgtt tctctgtaaa tgaaaaggca aacccggcaa aaaccacact ggcgatcggc
     3181 gagagcgttg ccatgaatgt ttctgctgtg gaaggtgcaa atatcaatgc atttactttc
     3241 aacggatgcc gcaatgtaaa tatttctgat aatatttatg ataaaggcgt gacacgcaga
     3301 gtcaatatca cgaatatgca gaacagtgaa gtgaagatcg gtgaaaacga aggcgtaagc
     3361 atcggcagtg gcacacagcc ggcaagcgaa atgatctatg tcagcagcga tccggaggtt
     3421 gtacgtgtgg atggcaccgg tcatgtgaca ggtctgaaaa agggaacagc aaccgtgaca
     3481 gcttatgaac tggtcggcgg acgtaaattc caggcagatc ctgtgacctt tacggtgaca
     3541 gatcagagca ccggaaccgt gacagcagtg aaagtgcttg caaaagatct gaccgtgaaa
     3601 aatccaagcg acggcggcta cagtgtcagc ggtgacagcg tgaccatcaa agcaatggga
     3661 cagggactct gggcaaccca gacagccaat aacgtgatcg taagcggtga aaatgtaaac
     3721 cgcggtgacg gaaccttcac agccatcgtc aagatgagcg gcaagaccgt caacaactgg
     3781 gacgaagcag gtctgtttat ctacaaagat gatgataact atgtggcagt cgagagaaaa
     3841 catggcacga acagtccaaa ggttcatgtc gtgaccgaag caaacggaag tgcaaatgaa
     3901 gatcagaaca cagaaaatcc gaatgcagag gcaatctggc tgaaactgga gaaatccgga
     3961 aataccttta ccggatatta cagtgttgat aaagaaaact ggacaaaagc aggaaacagc
     4021 atcacaaacg ataaaatcgg caattccttt ggcgtggcac tggttgccgg taccggaaac
     4081 agcacaaaag gaaccgcatt tactttctca gacctggaga tcaatggcaa ggcagtagca
     4141 ctgaccgaag atgcagcaac cggaggagag agcggaaata tcgaagatct tgcaaaagta
     4201 aaatcaggaa atgcgattct ggagagtgca tctgtatccg gtgtatcctt cccgacattc
     4261 tcatctgaga caaagagcta tgtgacaact gccgataaga atgtaaagag tgtaaagatt
     4321 tctctgaaag caaaagatga aaatgcaaaa atcgaaatct gccgaaatgg tgtcggtaat
     4381 ggtgtcggac tgaaaaatgt caatgagatc gtatccgcag aaaacggcaa aaaccagatc
     4441 acagatcaga aagttgcact tgtggcaggc ttaaatatca tcacaacaag agtgatcgca
     4501 gaagacggaa caacacagga aatttatcgt ttcctggtca cacgtaccgg agcacaggat
     4561 gcatccatcg gcggcatcag cgtagataac aaagaagtgg caggctttga tgtttctgaa
     4621 aaatcctata gtgcatctct gacaaaaggg cagaccgaag tcaaaatcgc tctgaaaaat
     4681 cttggcgaag gtgcaaaagc agttctgaat gtaaatggaa aaacatataa cccgcaagaa
     4741 agcattcctg tttccggcga taacatgacc gttggtattg cgatcacacc agacggcggc
     4801 gtaacagagc actacagcct gagtctgcgt gtaccatctg acagcaatgc aaaactggaa
     4861 aaagtacagt ttggcagtac catccagagc aatggaaact ttaatgcaaa tctcaaagag
     4921 nnnnnnnnnn tacaggttac agtagaatcc gcagacaaga gcagaaccga aacctatatc
     4981 tggagcgtcg aaggcaagag cgaagtttat ctttctgatt taagttacga gagcaattct
     5041 gcaactggat ggggttccat catgaaagac aaatctgtag acggaaagac actgacactt
     5101 tatgacggaa gccaggaaaa aacctttgag aaaggtatgg gtattcatgc gaccgcaaat
     5161 ctctattaca atatcgaagg tatgggcttt aagaaattta catcttatat gggcgttgac
     5221 cgtgaagcaa accaggatgg aaatgtaaaa ttcaatgttt atacggataa taaacaggtt
     5281 tacacaggtg aagctgttac gagagcatct gagatggcaa aattggatat ttctgtggaa
     5341 ggtgtcaaaa tcctcagatt gaacgtggat cagaatggca gcgacagcaa tgatcacgca
     5401 gattttgcag acgcaaaatt catcacagag ctggcagatg acactactcc ggaaacgata
     5461 gccgtaaccg gagttacact ggataagagc acagcgaaac tgacagaaaa aggtcagaca
     5521 gtagatctta ctgcaacagt agcaccggaa aatgcaacaa ataaaaatgt gatatttgaa
     5581 acgagtaatg aaaaagcagc aactgtagac ggaaacggaa aagtgaccgc agtagcaaat
     5641 ggaaaagcaa ccatcaccgt aacgaccgaa gatggagaga aaacagcagc ctgtgaagtg
     5701 actgtggaga ttccaaatgt accagttccg gcaccggaag aactggtgaa tgaagtgaaa
     5761 aatgaaatcg cagcagcaga aggcaggaaa gaaagtgact atacagaaga aacctggaaa
     5821 gtttatgaaa aagcactgaa tgcagcgaaa gaagccgtag agcaaaaaac agcaaccgaa
     5881 gaagagatgc agcagatttt aacagctctt cgcaatgcag cctctgcact taagaaagaa
     5941 acaccatcta cggaaaaaga aacaccgtct acagaaaagg aaacaccatc tacaaatcca
     6001 cagacaccgg aaactccaaa cgatggtacg gtgaaagttg gtcaggtgat caaagcagaa
     6061 ggtcagtatg gagtgtttac ttataaagta accggaaaag acacggtaga ggtaaaatct
     6121 atcactgcaa aaggcaaaac aaagaagagt gtgaagatct ttaataagat caaggcatcc
     6181 ggaaaaacat ggaaagtaac cagtgtggca gcaaatgcat taaaaggcaa caagaagatg
     6241 gaaagtctga caattgaaaa aaatgtcaga aagattggaa agaatgcctt tgcaaattgc
     6301 agaaagctga aaaaagttac tattaagagt aaaaagatca ataccattgg aaagaatgcg
     6361 tttaaaaata taaataaaaa cgcaacggtc aaagtgccga aagcacagaa gaaaaagtat
     6421 gcaaaacttt tgaacaaagc gaaactttcc aaaaaggtaa agataaaata gtaaagggaa
     6481 agtaacagaa acaaaaggaa agaaaggggt aaaatattat gggacttatc agagcagccg
     6541 ccggagcagt cggcggaaca atggcagatc agtggaggga gtttttctat tgtgaggcga
     6601 t
//
LOCUS       DAWDWH010000105         6598 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5205_length_6598_cov_12.204493, whole genome shotgun sequence.
ACCESSION   DAWDWH010000105 DAWDWH010000000
VERSION     DAWDWH010000105.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6598)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5205_length_6598_cov_12.204493"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2187..2196
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3882..3891
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4835..4844
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1536 a         1664 c         1281 g         2087 t
ORIGIN      
        1 tggataattc cttactggct gtaagggata ttaaccataa atatattgta gtagtaaata
       61 tttggtataa atcagtacac ttaatcgtag agtaaggtac tctgcgaaat tatctgttca
      121 ttttatcaat tccctaatca tcaagaaccc agacattttt ctatgccggg ttcttgatac
      181 tctacttttt tttatttttc catgttctca agtatcttta atgtcctaaa atctcttttt
      241 cctttaaagt atctatccag tacattcaaa aacacttgat tatcctcact tgctctggca
      301 aatagcgtca ttgatgattt cagtttcatg ttatctggtt ttccaaaaac ttcatatgca
      361 tcgcttgtat ccaggctaag taaagcttcc gaaatttcga taatattatg tccgaggtaa
      421 ggatgttcca gaaatgcttt tgcctcatcc aggctctgaa tcgcataaaa ctgagaagta
      481 ctgctttttc ccaacccacg gatttgtgga aaaatatacc acatccaatg acttactttt
      541 tttccatttt taatttctgt taatgcactt tgataatctc gctgctgtgc tgttacatat
      601 cttgacaaat catactgatt caatattttc acctcacttt ctaatttttc agcatgatta
      661 tattctatat tttatagcat tatatgtcaa ttacgaaact tcctacttct tttctcccac
      721 accctctctt gctttctcca gcagcccttc cagggtcttc gccgcctgct ctgttgatgc
      781 ctttccaaag aaatgtcccc agatgccaac atatcgttct ccgtccatct cgaacacgga
      841 gaagatcaga taattactgg ctttggtctg ctgtgtgacg actttttcca gtgcaccgtc
      901 aaagaatcct ccaagtttgc tctcactctg gctcacgtaa tactttgcaa actctgtttt
      961 cgtcggacag gttgtaatca gcgagatccc gatcgccgca acgatcatga atgaaaataa
     1021 tgttcgaaat aatctcatct cttcttatct cctttcttct gcaagcaaaa ttcctttttc
     1081 tgagatactc agacaaccgt tctgtgatga tgtactcact ctggctggct tttctgcttt
     1141 taagcatttt acagcttttc tgtttttcat gttgatctta ccgccttttc gatttctgaa
     1201 ctgaaagatc ctctgaaaat cgtgactcgc atagtatgag ctgttgttaa atctgccaaa
     1261 ctgctgttca ttgggtctga cggttccatc cgaataaaga aggaagactg cttcttcctc
     1321 cggtatttct tccagagttg ccggtgcgtt tggattataa ttactggact cgcttacctt
     1381 cacgccggaa atcgggcatg gttccggttc ttttgcttcc acttttgcag tttcctgatc
     1441 ccggttctgc gtttctgtct ctgtcccttt ttttgtatga aatgtcatat ccagatcctt
     1501 ttgtgacgga tctttccatg tatcctcatt tccgaaatct ctgttctctt cctggatatc
     1561 ttcctcttca tccaggaaat aatcgtcatc ttctttctgc ttctgccatt cactgttccg
     1621 gtcaggtgcc ttttctataa aatcagcatg tgaaattggg ctgcttacgc tttctctgta
     1681 accatacctg ccattttcca tacactgcag ctgtccgcta atcttattta acattccata
     1741 cagggtctgc tgccgcttcc aggttctttc atgcaatgcc tgctgctgtt tccagatttt
     1801 gatctgaaga agtgtaagaa caatcacact gagcagtgtt aaaaatgtca gtaaaatcat
     1861 aataacccag gaaatccttc caactccgcc cgctgctgct cctgaaacgg aagtctgcag
     1921 catttctgtt gcagactcca gtgtttctgt aagcggagtc tcggatgttt ttgtaagtaa
     1981 catcagatga ctcatccact ccacctcctt tacggttctt caaacagtga gatacgtttg
     2041 agagcaagat cgatcatggc tccgcagagc atttcttctt ccacaaacgg tgttgccatg
     2101 ataagttctt cactctgctg ctgaagctgt gcacggagat tgtcaaaatt ttcctctacg
     2161 gttctggcaa tctgcgaatc cagcacnnnn nnnnnntgat ggcttcttcg attccggtat
     2221 atccaaaacg ttcaaacgct tcttcgaatt tctgataagt gtaatcacca atcagctttc
     2281 cgaaagcctg ttcataaagc tcgctctgtt tctcctgctg agccgccttt gttgcatgtc
     2341 tggaaaggcc tgccagttcg tcccggaact gttcttttgc ttcgttgtct gcatccatgc
     2401 cgcttccgta aagtacatcc agataatgtt cggtcggttc gccctgcacc atgatctctg
     2461 ctcttgtatg tgtgtgctcc ttcatatcga tcataccctg ggcaacactg agcttttccg
     2521 tatcgttatt ggtcagcagt ctgatccttc ccgtgtattc tgctccttca aaagacggat
     2581 cagatttcag cagatagttg atttcttcat acagacggga agtgaaacga tctgcaaaac
     2641 ctggtgcggt attgttcagt gcaagtgatc cttttccgac cggtccgagc aagatttcac
     2701 catcttcagc gagagcttcg tctttcaggg cacgtacgat cgtgcaggca taggccggaa
     2761 tgccgatatt caacctggct tctgtcaatt cctggacttt catctgaaat tcatccgcat
     2821 tcacactggt tacctgaaaa cgacattcag tgaaaagtcg gttcagtgaa agattaaagc
     2881 caagccgttc gtacagctca ccctgtctcg atgccattgc ctgtctgatc gcatcctctg
     2941 cctgtttttt cagctcccgg ctgccgccgc tgaagcaccg catcagagct gcagcattac
     3001 caccttcgta gaccttcgca agtgttccgt tcgttactcg ctgagccgca tacggcacgg
     3061 agctcagata cagttttcca ttgactctca atgagaaatc gctcgttgag tacccgatat
     3121 ccagactgcc gagagccact ccctctccaa taaatacgtt acccggcgga ttcagctgat
     3181 gccagacggc tgccgcttca ctttcagaac aaaatgtgat gttgtggcct tcctcaagct
     3241 gtgcttccgg aagcagatac tcattgacca tctcgatcgc accacggata atatccagaa
     3301 gctgttttgt gataccctca ccagaaccat tttccggata ggagatcata aattctaggt
     3361 tattatatgg aaccgaaaat ccctctcttg ccagtgccag aatcccctcc aggatcataa
     3421 ttccgatata ctgtttcaga gccatggaaa tcagctgtcg gtctctgact ttcagatttg
     3481 atcttgtcag aacttctttc atattgctga tgacgccgat attggtcatc gcttctgtca
     3541 tcgtaccagg agagtctttc aatgctgcaa ataaaacctg ttcgtccggt ttccagatac
     3601 gactctcaac cataagagcg gaccagttgc ccattgtttc cgggaagaat tgctgcagca
     3661 gggaatcaaa atgggttccg ctttcgccag agtccatcag gcgacgttct aacgtctgat
     3721 tgaactctgt tgttgctgtc ggtgtcagtg gaaaggtcag attttcatag gaaatcgctt
     3781 cggatttccc ggaaccttcg atcgatttca ggcggacact tgttgctcct gccggatcta
     3841 aggaaagata catcttctga tttttacgtt ccggtgtcaa annnnnnnnn ncttcccgat
     3901 cacacagttc catccgctca atacgcaggg aacgctgatg aatttcccag tctccgtcat
     3961 actgatttga agtaagcctg atgacactgc tgcccggatc ttccggaaac acgttccatc
     4021 ttgtctccag attatcgttg gtgatggctc ctgccgcaga tccctgtacc agataattat
     4081 attttgccag aataccctcc ggcaccatgt acgccatatc gatatagttg tccggctgac
     4141 agattcgaaa tccgctcttc cctgccgcat agattttgtg acagagctcc ccgtccagtc
     4201 ttgctctgaa ttcgatccat ttcgggatac catttaccgt ttctcgggca aatttgtaac
     4261 cagcttcttt aatgctacga acattctgca ccggcggaat gacaataagg cgatcgctga
     4321 tctctccaaa agtatcgttg atatcagaaa gacatcttgt tccggtattg atcagaaccg
     4381 gctgccgcaa aactttctct gaaacgctgc tgcccatgta attgcatacg gcaagcggat
     4441 ctgcttttgc tgaagtctcc tggatatatt tgataacctc ttccggcagg gtttctgcag
     4501 gttctgcccg gtaaagttcc atttctgcat cagtaagtgc accgccgata tagtcaagta
     4561 cttttccaag tacctttgca tttgctccct ttgcgatcag attggtaagc agtgcacggc
     4621 agagttttgt gatatgtgga ttcccggcca taaattcttc cggcacttta cagcattttt
     4681 ctgactgtgg gaatctgacg cccggcacaa aacatttcca cttcggaaaa tacatggcaa
     4741 gtacttctcc gtcgacagaa acctgaagca cacgatctgg ctctttttcc attccggtat
     4801 cttcttcgtc cagtttcagt tttctaagat ccttnnnnnn nnnnatcgat cggattgtct
     4861 ttatcaaagg tgtgaagtgt gtatttcatt tcagaaccgt tatacggagc ctgtgcaagg
     4921 cataagtaca gggaaagcag ctgtttttca tttactcctg aaatttcttt tttcgcactg
     4981 gacacgttga tcagccaggc gataacattt ccaaaggtac tagaaatgtt tttcgcattt
     5041 tgcctggtct cttcattctc gaccggagtc atgataaatt ctgcacattc ctgaattgtc
     5101 gctccatcca catccatgaa accatgatgt ctttcaatat ttttaagttc cgcgattctt
     5161 ctgtgttctt tagaaaatgc cataatgtct tctctccttt ctttcgctgc agatttcata
     5221 aatatctctc agaatatcct gaaatggtct tggattacca tgttcgaagt atttaacctg
     5281 tttacgcagc tcatccatgg taagcctggt gttatagata ttttcctctc tttgttcagt
     5341 ccttctgtcc gcaggacaca tggtcagctc atcaaactga aatcctgtca tccctccacg
     5401 ctcgcggata tctgcttttt tgccaatttc ttttcctatc atctcctcga ttctttctgt
     5461 attaaacaat gtcgatccat aagcgggcgt agaatctgtc tcccagtttt tcccggtcag
     5521 agaaatttct ccaagatact ccagaaacat ggtttctttg gcaagcagtg ctttcagagg
     5581 cttccggatc tcttcttcca gatcaacgct ggaatttaaa actgcttctt ttacttttct
     5641 cggtgtcttc actttaaaca tccgggcaag aaactctgct tcatagattt tatccggatc
     5701 attttcttcc ggtgcctgaa tcagctgtgg acgaagccag tagaacagtg ctgcatcaaa
     5761 gcgaagtctc gcagataaat attgtctgta ttcttccgga agtcccagat tctcccagtt
     5821 ggtgagagcg tttccaggtc ttgagtaatg tggaataacc ggacagtgct ttttcgatgc
     5881 gtgttccgtg ctgagactaa agaaatcctc catcgcagca accgcaacaa gctcgatcgc
     5941 atgggcatgt ttatactgtt ctctggaatc cgatgctttc gtggcctgta ccggacactg
     6001 atcatgctcc agcagataga aaagattgac tgcctctgca gaatcctccg ggaaattgcg
     6061 gagcgttccg gccaccagac cggcaacatc ctggtcaagt ccattcattg ccgggaagcg
     6121 gaaagcactg cctgtcatga tcacggcaat cttgaggttc tgttccacat aagcctttgc
     6181 ctgctcctgc tccattctca gatcttccat cactctctcc acgcacagct tccggagcat
     6241 ggccggatgg gtacaaagat tggttctacc ttcccctccg atgccgcttc caaccatcac
     6301 tacgcgtgtt tcgtatgtat tattagatgc aacgacactg tctgcgattg cccgaaatcc
     6361 ggcatttcta tcttcttccg tttccagtgc acactgcata gctgcggaag atacgacact
     6421 gccaagcgtc agatcacgat agtaaccccc gagaaggtct ctttccagct gttcttccgt
     6481 aagcaaaagc ccatttcgat accatgcagg tgcgattttt ttaagactca ttgtgttgtc
     6541 tctggtgata tatgctctaa gtgacttctt aatcatgatt gtgtcatggt tagtgaag
//
LOCUS       DAWDWH010000106         6584 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5217_length_6584_cov_9.418594, whole genome shotgun sequence.
ACCESSION   DAWDWH010000106 DAWDWH010000000
VERSION     DAWDWH010000106.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6584)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5217_length_6584_cov_9.418594"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    548..557
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2370..2379
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4227..4236
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1693 a         1678 c         1195 g         1988 t
ORIGIN      
        1 gaaaacagaa tatcctcact tttcagaaat ccatactgac aaattggata atctgcaaga
       61 aatgcttcga tcttttcaaa cagatccgct tcacttatct tcattttttt cacttctttc
      121 ttgttctgcc gcattttctg aacctgtcgt tttacagacg accgccgact ccagacgatg
      181 ttctttaatg gaaagaatct tgatatgcag tccgcatgca tcgacctcaa actgtgttcc
      241 atcggatggg atcgttccgt atatgccaaa gacaaaaccg ccaaaggtat cataatcctc
      301 caccggaagt tccacttcca gtgcctccgc agcatcttcc agatctacac cgccgtcaat
      361 cttccaggta tcctttgaaa tccgggagat tttcggctct tcggcaagct ctgccaaatc
      421 atcttccaga tccccgacca gctgctctaa cagatcgttc atcgtgacaa tacctaccat
      481 gccgccatac tcatccagaa ccaccgcaaa atggttatgt gagactttca tccgccgaaa
      541 cagaacannn nnnnnnngac cacatgatca accgtctctt cgcagaccgg ataaacggtg
      601 tggcggctgt cgtgaatggt ctcctcccac tgttgcagcg tctcatccgt ccacagaagc
      661 gtgatgtctg tccgatgcgt ggcaaactca tccactgcca tgtcatcaaa ttcaaacaga
      721 ttttggatga tctcattttc ctccaaatcg atcacacctt ttttgcttcc ggcatctgcc
      781 attttccgta tctcttcctc gctgacttcc accgcctccg catttggatc gatcccgaaa
      841 agacgtaaca gaagatttgt agtcgcattt aaaagagccg taaacggcga aaaaacgatc
      901 cagaacactt ccagaagctc gctcatccga aatgccagct tctgcccccg ttttgccgca
      961 agacgctttg gcagcaggat accaaagacc agcaccacat aggaaagcaa aaacgtgatc
     1021 agaaccagaa agatattatg ccagactttt gccgaaacca ggtgtgcaaa ttgctccatc
     1081 acaagaccgg tcatccacag tgcaagcaga tctgccgtaa aggcactgat cagaaatccg
     1141 cacagtgtga tcatcgcctg gatcatggtc agaaatttcg ccggctgtct taaaaatttc
     1201 aaaagtcttt ctgctttttt gtttttttct tccgccattt tctccagttc cgatgcatcc
     1261 agtgagatca ctgcgatctc cgcacaggaa cagaacgcat caatggcgat cagaaccagg
     1321 atcaaaatta tttttcctat caaaggactg tctgcaatca taaatgctcc ctcactctgt
     1381 cttttttgag aatcccgccc ccgcgatcag ctcctgttca tcaaacagct gtgccaccgt
     1441 ttcctcgtac gtcgagacac tcttcgtctt tttgatcttc tttgcatatt tcgaagaagc
     1501 ggtaaacata tctgccgcat agtaccagaa ttctttcatt ttaaacatgg catttttatc
     1561 atcctgaaat gcttcccggt atgcttcaaa aatgcagtca tggaatacac gaaacctcat
     1621 cttgtccatg gcatttttac cttttatttc accgatcagt tccggattgg caagcactcc
     1681 gcgtccaaac attactgccg gaacttttgg atactgctcc ctgaatcttt caaaatccgc
     1741 ttttcggaac agatctccat tgtaacagag actgtttttg ctgttttggc aggcatatgc
     1801 aaacgtttcc atatccggcg ttccattata atacatgcac tgtacacgtg gatgtatgat
     1861 cagttcttca aacgggaact gattgtatac cgaaagcaga tcctgaaatt cctctgcatg
     1921 ttccagaccg atcctcgttt tcactgaaat tttgatcttt atttcccgaa aaacttcatc
     1981 aaaaaaaaat tgcagtgtct caggatctga aagaaacccg gctcctctgc ctttggtgac
     2041 aaccgttctg gacggacaac ccagatttaa attgacttcc tgataaccat attcttccag
     2101 agcctttgtc atggcaataa aatctgctgc tttgtttgtc atgatctgtg ggacaagatg
     2161 gatgttctga ttatgctcgg gaaggatatc ggccttttct tttgcactga actgttttcc
     2221 gctgtttggt ttcggaacca gaaacgggga aaaatattta tcggccggtg taaaaagcct
     2281 ggtatgtgca ttgcggaaaa tatatcccgt gatcccctca agcggtgcaa gataatatct
     2341 cataatactg cttccgtctc ctctaatctn nnnnnnnnnt gttcatagaa atcccttgtc
     2401 ctgtcatagt ttcgcacata aaacgtattc aaaattccca tggataccgc ttttctgctt
     2461 tcatccgacg aatcgttttc cagaatctcc tcctgctttt tcagataagc atgccagtca
     2521 gaaataaatt tatcatggcg tgcaggatct gaaatatcga tccatttttt cactttcacc
     2581 ttgagccggt ctttcttttc acactcgtag acttgcagaa aatagcgaaa tcctttttcc
     2641 tcatagattc tgccaagcgg aaagagacga cagatatccg gtcttgcccc atggatcgtg
     2701 catcgcccgg aatcattcaa aaaagcacag gcttcctttt caccacgcat ggccaggttc
     2761 cgccagattc agttccacac cttttgtcat cagttctcca aaagaagcgt tcagctcctt
     2821 ttcaagccgg taaatatcaa gcggtgtcag cacgatcgaa gtgcccatcc catgacagca
     2881 ggcagaacat ccctcacatc ccctgcagtc tgcttttacc atatcatttg gcccatacag
     2941 ctttccgtct gaaatttctt ttatatcgat attacgtttc acctttctcc ctcctcatat
     3001 tgacatctgc tcctgttaag agtactataa atttcaagaa tattcaacaa aaatcaacca
     3061 aacaaggagt agatctatga accctgtaca ggaaaccgtt ttattatatt acccgaagaa
     3121 gccaaaatac ctgccgaaaa tcaaatccat cttcgttcag ctcggtatcc agtttcgtat
     3181 tctggatgcc gcatctactg ctcagaagat cggctatctc accggtcgta caggcttcga
     3241 aaaaagtact tccgatgtcc ctttttcaaa aattccacaa tctgtgctgg tgatggatca
     3301 tttttctggt gtgcggatgg atgtgctgtt ttcttatttg aaaaaagccg gcattccttc
     3361 catcgacctg aaggcgatcg tgaccgatac caatgcagac tggacatttt tcgcactcta
     3421 tcaggagatt gcaaaagaac atgcacggat gcatgcaaga agagcaatcg tcacacgtat
     3481 cgaagagtcg gattttggat gtgaaggacg accggatggg gtgattgcaa tggatcatgt
     3541 ttacctgcgt tatgagcagg aatccgaaga attctgcctg atggcagaag ataatcagct
     3601 ctatgcagat catatcgatg aaaacagtac ggttctcgta accgcagatg ggaaaattct
     3661 gccgctgtaa atacagcagt taaaatgaat cccctggtat atctctggtt gttttttcat
     3721 ataaataacc aatttcatac cagggggttt tttatgtctg tttttccgtt caaaaaagca
     3781 gctgtcacac tcttgcttct gacagccgcc tacttttccg gattcgggat ccattatctg
     3841 ttttcacagc cgaccacggt acttcctgcc tcatcttctt cagaaggcaa ctggggactc
     3901 agttttcagg aagaaggtgt gcggcctgtc ggcaacgcca cgccttctga gcttgcaaaa
     3961 tatcaggcag cctacgcaga tgaaacagac gaaaaagtca tttacctcac ctttgatgcc
     4021 ggatatgaaa acggcaatct gcctgctatt ctggatgccc tgaaaaacca tcaggcggtt
     4081 ggcactttct ttgtcgtcgg aaattttctg gaaaccagtc cggaacttac caaacgcatg
     4141 gtagcggaag gtcacatcgt cggcaaccac acttatcatc atccggatat gtctaaaatc
     4201 tccacaaagg aagcttttga aaaagannnn nnnnnntcga tcaccggaag cgaaatggct
     4261 cactactacc gcccacctca gggaaaatac agcacctcca accttgaaat ggcaaaagaa
     4321 cttggctaca aaaccttctt ctggagtctc gcctacgtgg actggattca agatgagcag
     4381 ccaacaaaag agcaggcttt ttccaaactt ttaagccgca tccatccggg agccgtagtt
     4441 ttgcttcaca gcacctcttc tacgaatgca gccattctcg atgaacttct gacaaaatgg
     4501 gaagaaatgg ggtatcattt cgccccatta tctgaacttg ctgacaaact gtagatcaga
     4561 ggtgcacaga agaatgccgg ggcattctta agcactacat aataaacata gcatcccgga
     4621 tatttatgaa ttgtaaccaa aatccaacat ttgttgggta tttatatagg aagtaatttg
     4681 atttttcctt aatgaaatgg taatccttca tcatcagcgc tcataaagcc atcaggactg
     4741 ttcctcgatc actatatctg ttgtatatac cttctgtcca tctttattcg tatagcttcc
     4801 tgtttgaatc cttccagcaa ttgcaatctt tgtaccttta tggaaatact tttctgcgaa
     4861 ctctgctgat cttccaaatg atacgcaatt aatataatct acggatgctt cgttgtctcg
     4921 cttgattctc ctatctacag caagtgtaaa tctggcaaca gctgttgcgt tttctccctg
     4981 agaatatcgc acttctgggt cacgtgtaag gcggcccatt aaaataactt tattcataga
     5041 attgattcct cctcaaaacc tttgttttgc ttcatattcc gcagaattat tatattctgc
     5101 ctttcattca ataacttttg tacctgaata atccgggcaa ttttcggtgt actcatactt
     5161 atccagatcg ccacattgtg ttttgcattc ttctttcttc ggacagcata tgcagcaata
     5221 gtccagacct tctgtgcaat ctaatttaca gtgtccaatc atcttcgtat tcagctccat
     5281 atctaatcgg cccttgctct cctaaaggtc ttctcctttc cgttgatcgt ttcattcgct
     5341 ttcttttttt ctgttcttga tagcgttcat atcttctttt cataacttct acactatcgc
     5401 tcttgaaatg acaccagaat ctgtctccat cctcatcttc tgcacaaatt cccgcgtcca
     5461 gcaattcacc tgaaaacatt gcgtgtttct tgggaatgtc atagcagctt ccaataattg
     5521 tcagattttt cttcacacat gcctgtgcca tttcccatgg atcagatgat aaccaagaat
     5581 taaacatctc gtgataacca atttcgtaat tttcaaggcg attctggatt gctttcacaa
     5641 ctgttcctgc catttggttg tatttttctt tgatctcttc ctctgtcatt tctattccga
     5701 acagtgtttc ttccgatgga aaccgttctt ctggtcctcc tggattccat aattcgagaa
     5761 ttacataagc cgcattcacc tcttttacat atcctttaaa actatgccaa gaatttccct
     5821 gcttggctcc agatatttct tggtctttct tcaagtgtct gtatgtaaca tacttttctt
     5881 cggtcatttt ttttatcctt tctgccctgt ttccacaggg caatacagtt tatcagttat
     5941 ccagtttccc ataataagcg gagcggaagc cgaagaacgc gctcgagtcg gaacgtggat
     6001 agttcaggcc cacgctgaac acaccagcat tcgaggtgtt gtaccagctg ccaccacaga
     6061 tcggcaaata ttctccgtct gtacaatcca tataacaata cgcatctggt tcccctgcga
     6121 ataatgccaa ttctttgagc tgctcgctat tgcagttaat ctgtacatcg ctccatcgtg
     6181 ttccagtata atcctgttca atctctggat ccgttgtaat gcagatgtta tcatcatcaa
     6241 ctgaaattct gatgattttt ccctcatcat ctctgattgc ttcccagcag tccccttcca
     6301 gcgtcaggtc aatatccata gcagcatcat tattctttgc cgtctccagt acaccatttc
     6361 tgattcgcaa tcctcttacc atttcccata cgttaccgca aaggtcatgc actccggtta
     6421 ctttatgatt atgtgtccat gttgccggcc ctgatcctgt ctttgttttt ccgctctctt
     6481 caatcggttc gcctttttct tccaaatctg catggtactt tccgtaattc gtatttccat
     6541 gtggcaaggt tccatctctc aggctgagat ttgcaagcag tccc
//
LOCUS       DAWDWH010000107         6567 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5233_length_6567_cov_9.842752, whole genome shotgun sequence.
ACCESSION   DAWDWH010000107 DAWDWH010000000
VERSION     DAWDWH010000107.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6567)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5233_length_6567_cov_9.842752"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3953..3962
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4349..4358
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1986 a         1291 c         1559 g         1711 t
ORIGIN      
        1 agtacagttc acagctttca tatattgatt caactgttca taaaaacgca tagaagcacc
       61 tcttttcatc aaatttgcaa aaacatcaat gaagcgtatt ttgcgttcgg ttgctatgtg
      121 cttattgtat cggagatttg caaaaaagga aagatgaaaa tatcaaaatt cccgatttgg
      181 caattgacgt ttcgggaatc ttacttgtca aataagcaga atcttttata ctgagagtaa
      241 gagagaacag gttgatttta tacaaaatgt ttatatctgg acagagcgaa aagaaggggg
      301 ggagaaagag gttctgacag atcaggaaag gagaaatttt atgaggaaaa taaaggagaa
      361 acacatggat ataacttgga aacgaaaatg gatattctgg ctgctgctgg cagtgatgct
      421 gctgttttta ccgggaaaga catttgcagc agaaaaagaa agcagagaag cagaggattc
      481 atacatttat catacggctt ttgatgcaaa ggcgagcctt aagatccgtc atatgaaaga
      541 ttacggagta ctggattatg aagaaacggt gacagtggaa gattcctgtg attatctgga
      601 cggagatgaa aatctggatg aaatccaggc gaaaattaca gcctatgatg agaaagttca
      661 aaaagtgtgt gaaggaatgc aggttgtttc cggtacggac acacgaggta ttgacggcga
      721 tacgattaac attgaatatg aagatacgga agaaaacgga cagaccgtta ccaccagaca
      781 tttttatcag acttacaaga tccggacaga ctgggtgctg gaaaaaatgg agatttcgga
      841 tgtgaatatt gtgaacagca cactttcctg ttatgccggt gatgctccaa aagcaaccgc
      901 ccaaaaaggt gacagtgatg cttcaaaata tgaaatagcc tatgaacgct gggaaaagct
      961 ggaaacaaat gcacagggac acctggaacc agtagcgttc tggtattctg atgagagcca
     1021 gtatattgct gcaactccaa aatttaccac tttcgaatcc ggtgcaatgt atatgtattc
     1081 tgtcttttta aaaacggtag atgggaatga attttcaaaa gatctgtcgc tgacattaaa
     1141 tggaaaacag atggacagtc ggaccattat tgtgggaaat aacggaacaa cctgttttgc
     1201 agatgccctg tacagtgccc ggtctgaaga aaaagtaacg ccgaaaccaa ttgaagtgat
     1261 tgaagtaaat ggagtggatt tttctttcaa agcgggtgat gctccgagat tcaccggaag
     1321 tgtgtcggaa gatgctctgt actgtatcga tcacgaacga tggagcaatt taaaagaagg
     1381 atggacttcc tcggactact ggaatggcaa gtatggtgat tttgacggaa gctggggtat
     1441 gccgctcacg aaattcaaag cactggaaac ctattattat ggaatttgcc tgaacctgtc
     1501 agccagagga tatcgggaag gatattattt tgataaaaac ataacgaagt tgaagatcaa
     1561 tggtcagctg attccgatac cggatctgga aaacagtttt gatgaaacag gggaaacggt
     1621 atggtttagt aacatcaaaa gtattgtgat cccgccgcag ggcagcacag caaatggaag
     1681 tacaacaaat ccttccgaca catcacagac atttccgaaa accggaacat gggtcaaaac
     1741 aaaggatgca ctttataaag tgacaaaagc cgatgcaacc ggatgtacag taactcttat
     1801 aaaaccggtc agcaaaaaca aaacagcctt taccgtgccg gcagccatca aaagtgagga
     1861 tgggaaaatc acattccgtg taacggagat cagtaaaaat gcatttaaaa agcatgtgaa
     1921 actgaaaaaa gtcaccatcg gaaaaaatgt cagcaagatc ggtgccggtg cattttccgg
     1981 ctgcaaaaag ctgaagaata ttaagattac atcgacacag cttacaaaaa aatctgtcgg
     2041 aaagcatgtg tttaagggca ttgataaaaa ggcagtcatc aaagtaccga aaaagaaact
     2101 caaagtctat aagagtattt tcaaaggaaa aggacaagca aagagcgtga agattaagaa
     2161 gtaaaaagaa agcaaaaagc atggaattac cgtgaaaatt cagggatttc atgctttttt
     2221 gctgcttatg ggcagtacat ctgtttcgaa aagtctgctt tccggaacaa aatggaaaaa
     2281 gacttgtgtt aaggtataag aaacaaggtt ccggggatta ttgttatgat atctgcgaaa
     2341 tcttttatat atgccgaagc cggcaaatgt cgccgaaacg aatcctgcgt ctgctgtttg
     2401 atgaaatgca gccgcttgca aaagttgatc caaagggaga tgcattgctg catcagatta
     2461 tggaaaagtg ctgggaactg tattattacg gtgacgagtc aaaagaagaa caaaacttaa
     2521 aaaagcaatt gcaggaaatc gctgatcaga ataaactggt gtggaaaaca gaatagaatg
     2581 tgtcagatcc ttgtgacagg tgaaaattga taaaactttc tcaatccaat gaagaagtcc
     2641 gccttgattt catatggcag ttatgctatt ctaatgatag ttagaaaaac ctaactaatg
     2701 tgaataaatc atggcaggac ttcttctgtc tttatgcgat cagaggcaga agacgaagtg
     2761 ccgattattt taggacgaga aagggactgg caatcatgaa gaatttaaca attgaaaaag
     2821 taattggaag ggaaatcatc gactccagag gcaatccaac cgtagaagca gaagtttatc
     2881 tggctgacgg tacgatcgga agagggactg caccaagcgg agcttccaca ggagagttcg
     2941 aggctctgga acttcgtgat aatgataaag aacgttttga cggcaaaggt gtgacaaaag
     3001 cagttgcaaa catcaacacc gtgatcaacg aagcactgaa aggcaaagat gcatctgata
     3061 tttatgcagt ggatgcagcg atgaaagcag cagacggaac aaaagataaa tccaacttag
     3121 gagcaaatgc aattctggca gtatcgattg caagctgccg tgcggctgcg actgcacttg
     3181 agatccctct gtaccgtttc cttggtggcg tgaatgcaaa ccgtctcccg gtaccgatga
     3241 tgaacatcgt aaacggcgga tgtcatgcat tatcttccgg actggatgta caggaattta
     3301 tgatcatgcc ggttggggca ccttccttta aagaatgttt aagatggtgt gcagaagtat
     3361 tccatgcact ggcttctatc ttaaaagaaa gaggactggc tacctctgtt ggtgatgagg
     3421 gcggttttgc accggcactg aaatccgacg aagaagcgat cgagacgatt ctggaagcag
     3481 tgaaaaaagc cggttatgag ccgggaagcg actttatgat cgctatggat gcagcttctt
     3541 ctgagtggaa gacaggaaca gtcggagagt acaaactgcc aaaggcagga acggtttata
     3601 catccgggca gctgattaat cactggaaag ctctggttga aaaatacccg atcatctcca
     3661 tcgaagatgc actggatgag gaagactggg aaggctggaa gaaactgacc gcagaattag
     3721 gtgacaaagt tcagctggta ggcgatgacc tgtttgttac caataccgag cgtctttcaa
     3781 aaggtatcga gcttggatgc ggaaactcta tcctgatcaa attaaaccag attggttcgg
     3841 tatctgagac actggaagcg atcaagatgg ctcacaatgc aggatacaca gcggttactt
     3901 ctcatcgttc cggcgagaca gaagatacca ctattgcgga tctggcggtt gcnnnnnnnn
     3961 nngccagatc aagacaggag caccgagccg aagcgagcgt gttgcaaaat acaaccagct
     4021 gcttcgcatc gaagaagaac tcggagcatc tgcagtttat ccacagaaaa aagcatttca
     4081 tgtttcaaag taataaaagg taaaacaaat cacacatata catacttcct gtaatctaca
     4141 tgtttgccgt ctgcaaagca tccataagct tgcggacggc aatgcttttt ggccggtttt
     4201 tgtcccagat caggcagatt tcgcgttccg gcattcttga ggtgaggtgg agctgtacca
     4261 cttcgccttt ttttatggct tcttctgcca gtgtctccgg ataaaatccg attccgaggt
     4321 tgtgcctgat catcggcagg atctgatcnn nnnnnnnnct tcgatatccg gatagaacgg
     4381 cagatcatgc tgcagaaagt aattgatatg catttctctt gttccagtat tttgtccgag
     4441 agaaataaac ggaatgtccg taagctctga gagatgatgg attttagagg aaaaatcagc
     4501 gtattttggc ccggcgagca gaatctcacg gaaggaagca agcgtataat gacgcatgga
     4561 ttttttcagc ttgagcggtg ttgtgacaac agaaatatcg gccagtccgt tttccagtgc
     4621 ctgaatggac tgaggggtgg agaaattgga aattttcagg cggactcccg gatactgtgc
     4681 gtgaaaggtc tccagtcggt caagcagcag cagtcgcaga gctgtctcgc ttgcaccgat
     4741 cgtgatcaga ccgctttcca gacttttgtc ttttttcagc tcggattctg cagtagagag
     4801 ctgctcgtaa gcgatagcga catgctggaa cagtttttct ccctcaggtg tcagtgttac
     4861 tcctcggttg gagcgtacaa acagtttaca ttccagttca ttctccagaa tgttcatgca
     4921 gcgggtgata ttcggctgat tgttatttaa aatctcggca gcttttgtaa aacttttata
     4981 ttgggctaca aaataaaaaa aacgataata ttcaaaactg accatgcaaa cctcctgttt
     5041 attttgacgt tcaaaaatgc ctctgcaatc cgccggatct gttctgtttt gggataatct
     5101 gaaatctgaa aaaggaagca cttttgcagt gctcccttat attcagatta cttgtgaagt
     5161 cacagaacat cgccggactt cggatgaaaa atatttacag aattattcac caaggttttt
     5221 cttttctact tcagcgagca tcatagcacg tactttcaga ggcagaccaa acagggtgat
     5281 aaatccgctt gcatcattgt gatcgtacat atcaccggtt gtgaaggatg ctaaagattc
     5341 attgtacagg ctgtatggag aagttgtgcc ggctttgatg atgtttcctt tgtaaagttt
     5401 gaatttaact tcgccggtta catatttctg tgttacttca acgaaagcct ggattgcctc
     5461 acgcagcggt gtgaaccatt ttccttcgta tacgacctga gcgaactggc tgccaagttt
     5521 tttctttgtt tccatggttt cacggtccag agtcagttct tcgagctgtt catgggctgc
     5581 catcaggatg gttccgcccg gtgtctcata tacaccacgg gatttcatac caacaacacg
     5641 attttctacg atatctacga taccgatacc atgtttgcct ccaagagcgt tcagctctgt
     5701 gatgatctca gaaactttca tggcttttcc gttcagtgtt ttcggaaccc cctgttcaaa
     5761 agtcatggta acatattccc ctttttccgg agcctgttcc ggtgtcacgc caagcaccag
     5821 catgttttcg aagttcggct cgtttgccgg atcttccagc tccagacctt cgtggctgat
     5881 gtgccataag ttacggtcac ggctgtagct gtggctgtgg tcgaacggca ggtcgatacc
     5941 gtgtttttta cagtattcga tctcgtcttc acgggactgg atggtccata catcggtcat
     6001 acgccatgga gcgatgatct tcagatccgg agccagtgcc ttgattgcca gttcgaaacg
     6061 aatctggtca ttacctttac cggtagcacc gtggcagatg gtagtagcac cttctttacg
     6121 tgcgatctca accagtttct ttgcgataac cggacgtgcc atggaagtac caagcaggta
     6181 tttgtgctca taaactgcac cggcttttac gcaaggcatg atgaaatcgt cacagaattc
     6241 atcaatgata ttttcaatat ataatttaga agcaccggac agttttgctc tctcttccag
     6301 accatccagc tcgttaccct gtccgcagtc gatgcagcag cagataactt catagtcaaa
     6361 ggtttccttt aaccaaggga tcagtgtggt ggtatcgaga ccgcctgaat atgccagaac
     6421 tactttttct ttcatgatat gtattcctcc tgaaatttat gatcattttg taagtcaaag
     6481 acaaaagagc cataaaacca ctcttgtgca agtacatcgt ggtttttggc tttcgaccct
     6541 ggtatcatac actatacaac agttatc
//
LOCUS       DAWDWH010000108         6554 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5248_length_6554_cov_9.979381, whole genome shotgun sequence.
ACCESSION   DAWDWH010000108 DAWDWH010000000
VERSION     DAWDWH010000108.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6554)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5248_length_6554_cov_9.979381"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1892..1901
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4173..4182
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4669..4678
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1603 a         1535 c         1502 g         1884 t
ORIGIN      
        1 ctgctcgcaa agccggatct gatcctgctt gacgagccga ccaaccacct cgatatgcag
       61 tcgatcgcat ggctggaaaa ttttctgatc aattacagcg gtgccgtgat catcgtagcc
      121 catgaccgtt atttcttaaa ccgtgtcgta agcaaggtta ttgaaatcga ccatgcgaag
      181 gcgaccacgt ttcttggcaa ttattctgct tattctgaga aaaaagccca gctgcgtgat
      241 tcccagttaa aagcatggat gaaccagcag cgtgagatca aacatcagca ggaagtcata
      301 gacaaactga agtcctttaa ccgtgaaaaa tcaatcaaac gtgcggaaag ccgtgagaaa
      361 atgctggaga aaatggaagt cctggatcgc ccggatacgg aagtgcagga attaaagctt
      421 tctctggaac cgagatttcc aagtggaaat gatgtgctgc gtgtcgaagg tcttgcgaaa
      481 tcatttggag accacacgct ctttacggat ctggattttg agatcaaacg aggcgaacgt
      541 gtggcactga tcggcaataa tggaaccgga aagactacga tcttaaagat catcaatgag
      601 cttttggctg cagatgccgg aagttttacg cttggaagca aggtgtgtat cggttattat
      661 gatcaggaac atcatgtcct tcatatggaa aagacgctgt ttgaagaaat ttccgatgat
      721 tatccgacac tgactaacac ggaaatccgc aatgtgctgg cagccttttt atttaccgga
      781 gatgatgttt tcaaacggat ctctgatctg agcggtggcg agcggggccg cgtttctctt
      841 gccaaactga tgttgtcgga agccaacttc ctgatactgg acgagccgac caaccacctt
      901 gatattttat caaaagagat cctggagcag gcactgaacc gctataccgg aacagttttc
      961 tatgtatcgc atgaccgata ctttatcaac cagacggcaa ccaggatcct ggagctgacc
     1021 ggaaatacgc tggtcaatta tatcggaaat tatgattatt atctggaaaa gaaagatgag
     1081 ctcaccaaaa tctatgtgcc gtctgccaca gaagaagaaa ctgcttctct tccatcctct
     1141 tctgcggaaa ctgccggaaa actgacctgg cagcagcaga aagaagagca ggcacgcatc
     1201 cgcaaacgtc agaatgaact gaagaaaacg gaggaccgca tccatgtgct ggaagtccgt
     1261 gataaagaga tcgatgaact ggaacagctc tacgaacgct gggaagaact ggcagaataa
     1321 aacaaaaagc atcggagaat gaaaggtact ttcttccccg gtgctttttt atagaaatat
     1381 aaaaatgcgt tatgtacttt ttacacgttc ttttacagat tcttatccag ctcttcacgt
     1441 actgccttga taaatccttc gctgttcaga acggtaggat tttcaatgct ggtgatcagt
     1501 gccaggtctt ttgtcatctt tccgctttca atcgtggtaa gggttgcttt ttccagtttg
     1561 tctgcaaata cgccaagctg cggaatctta tccagctcac cgcgtttgcg gagtgccccg
     1621 gtccatgcaa agatcgttgc cacggagttc gtggatgttt cttcgccttt caggtattta
     1681 taatagtgac gctgaaccgt tccgtgagct gcttcgtact cgaaattgcc gtccggtgat
     1741 accagtacgg aagtcatcat cgccagtgaa ccaaaggcag aagaaaccat gtcgctcata
     1801 acatcaccgt catagttttt gcatgcccag ataaatccgc cttctgcttt catgacacgg
     1861 gcaactgcgt catcgatcag agtatagaaa tnnnnnnnnn natagaaata agtaatacct
     1921 gcttctgcaa aacgttcttt atactctttt tcgtaggttt cctggaaaat atccttgaag
     1981 gtgtgatcgt attttttgga aatcgtatct ttggtagcaa accagaggtc ctgtttggta
     2041 tccagtgcat agttgaagca gctctttgca aaactgcgga tggatttctc tgtattgtgc
     2101 atgccctgca gaacaccagg accgtcaaat tcatggatca gttctcttgt ctcggtacca
     2161 tcctctgcgg taaagaccag ttctgctttt ccggcacaag gtactttgat ctcagaagct
     2221 ttgtaaacgt caccataagc atgtcttgcc agtgtgattg gctttttcca gttgcgtacg
     2281 catggttcga tgccctttac aacgatcggt gtacggaaaa ccgttccgtc catgatcgca
     2341 cggatcgtac cgttcggact cttccacatt tctttcagat tgtattccgt catgcgggct
     2401 gcgtttggag tgatcgttgc acattttaca gccacgccgt attttttgtt tgccagtgca
     2461 ctgtcaatgg taacctggtc gttcgtctca tttctgtgct ccagtcccaa atcatagtat
     2521 tcggttttca ggtcgatata cgggagcaga agctcatcct tgatcatttt ccagaggata
     2581 cgggtcatct cgtctccgtc catttcaacc agtggtgttg tcattttgat tttgtccatg
     2641 atgtcctcct ttgacttatc agaaagctga ttttattcct catcggaaga aacgtcgcct
     2701 ccgggctgtt cttccagaag tttttcgatt ttggtataat gatggttttt gattgaattt
     2761 aatatatgtc tggttgccag gcggtctgct tcatcggcat tgtgggcttt gatggcttcc
     2821 ataacagcca tatgttcttt tgtggactgt tctgcccttg cttctttttt cagggcgttt
     2881 ttgcgcacct gctgtacata ctgatggaag atcttcagtt catgttccag gatcttactt
     2941 ttgcatgctt cgtagagcag actgtgaaaa cggctgtcca gtttaaagag ctgatcgtaa
     3001 tgtccctgct cttcatgaaa tgcagtcagc atcatggtct cttccatttc ttccagtgtg
     3061 gattcatcga tccgctctgc cgccatcctg gcacaaagcc cctccagcct tgcacggatc
     3121 aggtaaatat cctgtacatc ctgttcggag attccgttga cgaaagcacc gcgattgggg
     3181 atgatcgtta ccagaccttc cagttccagc tgacgaagtg cctctctgac cggtgttctg
     3241 gaaacaccga tctcttttcc gactgcagtt tcccgcagtt catcgccatg tttataatga
     3301 ccgtttaaaa tgttttcacg aagtttttga taaaccaatt cataactgct caaaatacgc
     3361 tacctcttat actaacgcaa tggaaagctt tttgcaactt tcatcgatca cttttaccag
     3421 ttcgttatcg gtgatcacgg tgacacggcc ctcttcgtac tgtttgtcca cccattcttt
     3481 taccatagac acaagaggca tatcctttgt cagttttttc tcatccggca ggcggtaata
     3541 actgttgatc cagtgtgcaa ttccggcaag cccggatgta ttggatacag ccaccatagc
     3601 cgggcggttc aggaatttct gagtattaaa aatgttgtaa atctcttcgt ttttcagaag
     3661 tccgtccgca tggatgccgg ctcttgttac attaaagttg cttccgacaa acggtgtacg
     3721 ggatggaata tgataaccga tctctttttc ataataatct gccatttctg tgatgacggt
     3781 ggtgtccatg ccgtcgaggg taccgcgaag ctgtgcatat tcaaacacca ttgcctccag
     3841 cggagtattt ccggtacgtt ctccgatacc gaacagagaa cagttgactc cgctcgcacc
     3901 gtagagccat gccgttgtgg aattgctgac agccttgtag aaatcgttgt gaccatgcca
     3961 ttcgatcatt tcagaaggaa caccggcatg gaccatcaga ccgtaaatga taccctgtac
     4021 ggaacgcggg atcacagcac cggcataatt gacaccataa cccatggtat cgcagcagcg
     4081 gactttgatc gggatctgat attctttgga cagtttcatc agctccagac agaacggaat
     4141 gacataaccg tagatatctg ctcttgtgat atnnnnnnnn nnggaccagt tcgaaatcct
     4201 ttttgctggc acggatccag cttgtgatct caggaaattc ataaccctgc tccagacatt
     4261 tgtaaacagc atcccggtct tttttgctgt acaggaaaaa ttcgcactga cggatctttc
     4321 ctttcggacc tccaagccga tgcatataat catagatacg tacgatctgt tcggttgtgt
     4381 acggtgcacg tgactgctgt ccgtcgcgga aggtagtatc tgtgatccag atctcgtccg
     4441 gcatatgatg cggaacgata cggtcattga atgcgatctt tggaacttct tcgtatggga
     4501 acagattgtg gaacacattc ggtgtcttga catcaaccag cgggtacata tgctcctcaa
     4561 gctgtaacag gtttgtgtgc gaatcaaatt ttacttttct gttttccatt tatattacct
     4621 tccttgattt gtagattcgg cgttttgccc tttttgcata attgtatann nnnnnnnnat
     4681 ccctgctgat atttcggcag attcagattt cgggcacaat ttttaagacg cattttcact
     4741 tccacgtttg taagatgtgg ttctttttcc agaagcaggg cgatcgcacc ggagacaacg
     4801 ggtgctgcca tggaggtacc gcttttgata cagtattttc tgccgtccag ataccaggtg
     4861 ctgctgcagg atcggatgtt tgatccggga gccgtgatct ccggtttgca gatacattcc
     4921 tgtgtcggtc ctctcccgga atagcagccg cccggaaaaa tgcagtcatc aaaggctccg
     4981 actgtgatca ctttcttgtt tgttcctgga actgttacac tgccggcgtg tggccccata
     5041 ttaccggcag caaccaccac cacaagtccg gcatcccagg catgttccat acattctgcc
     5101 agtacggcat tcttttcctc caggctgttc accgcaccga cggaaacatt taaaattttt
     5161 atatgatagc ggtttttgtt tttcaaaatc caggacacgc tggataacac ctgttcccgc
     5221 atgccgttcc catggtagtc aagtaccttt gcacttacaa tcccggctcc cggtgccatt
     5281 ccggcatact ggcctctgga aagctgtccg ctcccgcagc agatccctgc cacatgtgtg
     5341 ccgtgtccac tgtcgtcgta cggatatttt ttcccgttct gaaaatcacg gaaagcaagg
     5401 atcctgccct gcagatccgg atgcatgctg ataccggtat ctaaaaaggc taccgtaacg
     5461 ccttttcctg tgagatgtct ttgatatgca gatgtggcat tggtttttcg tctgacctga
     5521 tccataagtg tccaccgttt tttcattact atactatatg ttgcttttca ctcataagat
     5581 aatggaaatg acgatggaaa gaggaatctt tatgcctgtt tccactacag aaaagattct
     5641 gtcggaggat tattttgatc tgattgtccc cattaccggt tccgatactg attttgaaga
     5701 ggcatttttc cgattcgggg cacaggcggc tgttccagga tacggtatca ttcacattaa
     5761 ccggcagttt cttccggata atattctgaa tgtgatcgga tattccaata ttcccaaact
     5821 ttataccctg atggatactt ctgcactgga aagttccgga atcctaaaag tgcagacaca
     5881 gccgcttctt ggctatcagg gggaaggggt tctgattggt tttctggata ctggaattga
     5941 ttttacgcat ccggcatttc gtacatctga cggaagaagc aggatttatc gcctgtggga
     6001 tcagacaatc caaagtggaa cagctccgga tggatttttc tacggatcgg agtatacaaa
     6061 cgaaaatctg aatgctgcgt taaatctgga ggatccgctg gaaactgtgc caagtgtgga
     6121 tgaaaatggt catgggagct ttcttgccag tgtagcagcc ggaacagccc ttcctttgca
     6181 ggatttttcc ggtgcggcac ccaaatctga aattgtggca gtaaaactga aacctgccaa
     6241 agagaatctg cgggattttt tcctgatccg tgagtcagcc gtcgcctatc aggaaacgga
     6301 catcatgctg gcacttcgct atctgcttca gtgtgcaagg caagctcaga aaccacttgt
     6361 gatctgtttt gggcttggaa ccaatcaggg agaccactcc gggtatatgc cgcttgcaga
     6421 ggctcttgat accataagcc gcatgaatac tgtctatgtc gtttctgccg ccgggaatga
     6481 ggccgggagg ggacatcatt tttacggaaa aatggaaaat acttccgatt atcagaatgt
     6541 cgaggtactt gtcc
//
LOCUS       DAWDWH010000109         6504 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5279_length_6504_cov_14.565824, whole genome shotgun sequence.
ACCESSION   DAWDWH010000109 DAWDWH010000000
VERSION     DAWDWH010000109.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6504)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6504
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5279_length_6504_cov_14.565824"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2743..2752
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1525 a         1681 c         1233 g         2055 t
ORIGIN      
        1 gtgggaaaaa ggtctttccc cgaaagaaag acctccatat tcctttaaat attttaatgt
       61 attatccgta agtttcatat tctatcacct gcagatttta aagataaaag ctttccgttc
      121 accgatttct gtgaactgcc gctgtcaaag ttccagtctt ccgacttctt tgtaatcgtt
      181 tgccatgtca taaatacgga tctgtccgcc gccgaccaga taaaaattat cgccgataaa
      241 acagccccgg acttcctgaa gtccgttgta agagtagcca tagtaagaat ctccgagatt
      301 ttccgtgaag acattttcaa atcctttttc ttcatcatag caaaagacca tatagctgga
      361 atcacacatg aatccaaaaa catttttttc cgtatcaatc atcgccgctt tgtaattgta
      421 gaacagcggg cagtcgtaag tgtctttgat cacaaacttg tgcagctgtt ttacgttgga
      481 cggatcggat acatcaaaca tcgcaagttt gatacctgta taagctcctg tctccggatc
      541 aacttcgttg cccacaccga gcagtttgtt ttctccgtaa aaatgcagat acgaggaaaa
      601 tcctgtgatt ttcagttctc caagtatttt cggatcagac ggatcgctta agtcaaccga
      661 aaacagcgga tctgtattgc gaaatgtcac aaaatatccg atatcaccga gaaatcttgc
      721 ggaacggatg tcttctcctt ctgccagatc tccaatgttt ccgcagacct gcatcgcatc
      781 atccagcaca tagagtgcat ttttctgtgt ctccccgctg tagtctgtca gaacaactct
      841 gagataactg ttgtactcat tcatagaaaa actgtcgttc agagaacctt ttagatctgc
      901 tgcacctttc gcctggatct tccctttctg gcaggagaat ttcagaatct gtgtcatcgt
      961 cacatcactg ctccagtttg tgttgctgat ataaatatta ttctcactta cataaaaatt
     1021 ctccgctgca gatacaactg ctttctgatc tatgatcgca tccggttttc cggtgctcac
     1081 agaagagatt accaggcagg aagtactgtt ctgatactcc ggaagaaaaa tatccgagga
     1141 ctccattctt gcaccattta ctgccggaat gtactcatca atttccgatg catcgctgac
     1201 ctgcggatca aactgcgtaa acagataaac gatatcgccg ttttttctgg aagtgctgta
     1261 gtgtccctgc tgctgaacca caccggtgag ctgcggctgg gaacgatcgg aaatatcata
     1321 ggtgtaaagt tttgtataat tttccgtatc cgccgtgtaa gtatcttctt cagcatcact
     1381 gttcagcgta gtcttgcttc cggtcacgat caggctcagc gtgtcgccgt ccacgtacat
     1441 ttcctgcggt gtctcattca atgcatccag catgatcgtt ccctctgtct gcaaagcctt
     1501 gtcttctgca cggattagac ggacactgcc ggttgctttc atcacataca gatattttcc
     1561 gtccgtcttg actatatctg cttcatccac gcctgcttcc tgcagatttg tctttgaata
     1621 agaaaaccct gctcccgcat ttgtttcaga gctttccata tccatggccg ccatcgccga
     1681 atcttccacc atatagtcgt ttgtcgtacc tcttgccagc tctgctgtct catacccgcc
     1741 atatccgctt tctgtttttt tcagggcatc aaaaatctcc tgataactct ttgcaccggc
     1801 aaaaacataa ttaccggatt caacatccgc tgacgtctgg gccgtcgttg ttttttctgt
     1861 tgttgcttcc gttgtatttt ctgttgtaac ttcaaaaatt tctgtctcac ctgtcgcttt
     1921 tgtactctgc gatgcgtctg caagcgacat cgttccggct tcattctggc tgttttcgcc
     1981 atttgtgccg gaaagtctgg taagacccgt cacccctccg atgatcacca gtgcgaagac
     2041 tgccgcaact gctccccact gggcatatct tcgaaaccgg ctttttttat ccaccggctg
     2101 ttcttccagc agacgtttaa tctgctctgg atggatttct tccggtgcat caacattttc
     2161 accggatttt tttatcatat ccaaaatctt tttttcatcc atacaatcca actcctctca
     2221 ctcactaagc atctgctcca ttttcttaag agcacggctt ttcagagaac gcacggtatt
     2281 ctcattcatt tccagcatgg aggcaatctc ttttccggta tatccgccaa acagactgga
     2341 agaaagtatc agacgttctt cgtcatttaa tttataaaat gccgcccgca catccatatc
     2401 ttcgctcgtg tcacgttccc gccatgccag atcggccgga agctccacgg cacggttcag
     2461 ataggatttc agcttctgtc tgcatttgtt ggcaagaatc cggaaaatcc aggcacgaaa
     2521 agcattttcg tcttttaacc cttcaatccc cttccaggca tccagtaccg tctcacttac
     2581 aacatcttct gcttcatgcc tgtttttcag ggtatataat gcaaatcgaa acatatcctg
     2641 atatactttc tcgtattttg cagcaaaatc ttctgcattt cgctccatat gatcacccca
     2701 ttctttttat gctcaggagg cgttccgtct gcagccttcc cgnnnnnnnn nncccgcctc
     2761 tcatctatac agtgtcattt ttttcgtgtt ttgttgcatg gaaaattttt tctgttttta
     2821 ctcctcaagc ggatcaaaat acgttacaaa ctgatttccg gctgcttcaa acatatcggc
     2881 actttcttcc agtccgtaat aataggtctc ggcttctccc ggttttaaca gaatcgtatt
     2941 gtactggtca tatccggcaa tgatcacaac gccgtctgct gtttttgcca gaaccggcgt
     3001 gccctcgctt acataataca gcacactgtc gagtgtacat ccggtcaggt caagtacctg
     3061 tttgccgaga ttttttgaca gttttttgat gttcatctta ccgcttaaaa tcacatccgg
     3121 gaacgtactg atatccagct tgcaggtggt ctgtttgttg cctcgctccc agatcagctg
     3181 ctgtttgttg tttaccacaa cgccaagctg atcatctgct ctctgtaccg cctcactgat
     3241 cgtggtgtac atgctgtcga gatgaccttt cgcatagaca tagtaaagtt cttctgtctg
     3301 tttttgggcc ggaatcgata cttctgcagc ctcttcgcct tctgccttga cctgttttgc
     3361 ataaaggatc tgcggtgttg taccttcctc gatcgtctca cccgttttca gaatggtttc
     3421 tgtctgtttt ttgtcgcttt ctgcatacgc aaatccataa gccgcttcat cgtccgcact
     3481 gctgtttacg atgtgatcct ggctggtctc tgtatagccg cgtttcgttt tcttgacacg
     3541 gtccagcgtc agcagtttgt catttacgct tccgccgatc acgtaacaac cgtccggctc
     3601 ataagtcttt aatgtctctc ccgcagaatt tacgatcagt actttgtaca tcgggaatac
     3661 ttcgcttcct ttgtcttctg tgtcaatatc cgaaaccttc gccacgccgt ataccaagtc
     3721 agaatcgata aatccgattg gctgcagacg ttcatcactg tcgcaggtaa atgcagtatc
     3781 gtttcctgtt tccagatcac gcagattcag cgtagaagaa tcgtattttt cattttcctg
     3841 aagccatgca aattttcctc cggtggaaga gccgacataa catccgctgc ttaagttgga
     3901 aaactgttct gtcactgaat tatctgtgat attgatatcg taaaaacttc cttccagtgt
     3961 cagataaaaa tgtgtccggt ccgctgacat ataactcaga ttttcgacat ctttatccag
     4021 cagggaaaat gcttcctgtg tttccacgta aagtcgctcc gtgatgctcg ttgtcgctgc
     4081 attgtaagta tagaccgcaa cgccgcattc tccttcatgt ttgccacggt tcatataacc
     4141 gcacagaagg aaggtcatat ttccgctgga atctatattt atgatgcgga tattattttc
     4201 atcataaata tctctcgcat ccagctttcc attctgaagg aaactgaaca cctgcgtcag
     4261 ctttttcgtc cccgtttcat aaagccacag ggaaccctgc tgtgcaaatg caaaatattt
     4321 tttgtcggta tcacttttgt aggtcagatc ccgactgttg atgccaaggc ggataccatt
     4381 tgtcacaaga atcgaatttt ccggatcaaa aatttcattt gtgtcacgct caaaatcaag
     4441 cagcatgacc tgagattctg cgtatcgcat acgatagtat tctgtcacat gatacatttc
     4501 catctcttcg gtatcaccgg tcgctgtgat cacataatcc atttccagtg ttgctgtgtt
     4561 ttcgtttaat tccttgatgc tcgggatcgg ttttaaatat gcctgcggcg tgagctttcc
     4621 ccacatcagc tgtgcaccgg aactgtggat gtccatatgg gcaagcgttg tgttatcggc
     4681 atcttcattt ggctcgatcg tctgtgagat cattccgtct tcatcattcc cgtttaaaca
     4741 tttctcataa aatccggttg caaaattcag gtagttttct gtattcaggt tcgcctggtt
     4801 gataatgcgt gtgtaataat aaatatccct gacaccggca gtcactttga tcttcatgat
     4861 atattctgtg ttgatcagga tcttattatt aactttcagt tctgccgtca cataatcatc
     4921 ctgtttgcca agtgtcgtta ccagcgtatt ttccagtgat ttgctgccat ccgcagacag
     4981 tacttcgtag ctgacgctgc tgatctggtt ttcaaacggc tggatctgta tatttactgt
     5041 tttataatta ctgagcggtg tcagtgtatc ccgcatggca atgacatcca tttcctgtgc
     5101 ataaccatgc atacagttga tctgttttcc gttcagatca acataaacca gcggaaacgt
     5161 agcctgtgcc atcacttcag aggtatctgc catttttttt ccgaggattt tggatgtacc
     5221 aaagacagcc gccgcaaaaa caagcaccag aactaagatt cttatcaata ttttcttcat
     5281 ttacatttcc cctttttctt ttctcaaacg tccaagcagc ggatcaacac caagaaattt
     5341 ctcacaggca cccagttctt tgatctcatg tggttttccg gtttttactg ctcttataag
     5401 aatatttttc ggagtatgct ccatatcaat aaactccaga atctgcacct gatatccggc
     5461 ttcttccagt cttcctgccc ggagtgcgtc cgtcacaagt gcagcgatcc gctccttgat
     5521 cagaccatat ttcagaactg gtttcagaat ctcattttcc atctgtttat tcagttcatg
     5581 ctgacagcaa ggcacagaca ggattacttt tgcatcccat tccagagcct tctccagagc
     5641 ataatctgtg gccgtatcac aggcgtgcag cgtcacaacc atgtccactt tggaaacgcc
     5701 ctcgtaatct gcaatatctc cggtcagaaa agtcagttta tcatagccgt atttttccgc
     5761 aagggcattg cagcgtgcga tcacatcttc tttcagatcc agtccgacca cacggatatc
     5821 atagccattt aaaatcttca gataataata aagtgcaaac gtcaagtaag atttgccgca
     5881 tccgaaatcc agaatcgtaa gctctctgcc cttttccatc tgtggcagga tatcctgcac
     5941 aaactccagg aaacggttga tctggcgaaa tttgtcgtat ctggcatgca cgatcctgcc
     6001 ctctgcagtc tgcactccaa gatcaatcag aaacggcacc ggaatccctt cctccagaat
     6061 gtaatgtttc ttacgattgt gcatcagaac cggtgcatcc agttttgtca cactcttttt
     6121 acgtttgatc gtcacttttc cctttttgct gatcagaact gtgatcagct cgtcctgcat
     6181 ctgaatctga agctgccgga aatcctcttc cataaatttc ataacatccc cgatcagctc
     6241 atcacgatcg cggttgctgt gaaactcctg cttccccttg cgtgctgcag actgaaacat
     6301 cagacttcct ttttgcagaa ccggccggat acgcactttg gaaatgccat ccttgtccct
     6361 cgccccactt gctgtcatat gtatcagctt ttcacagaca cactcctcca gcaatatttt
     6421 taattcttcc atatatcctg taccctttct tttcaagtcg aacgcacgtg agacttggtg
     6481 cgtgattctg gtcagcgaaa gctg
//
LOCUS       DAWDWH010000110         6395 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5355_length_6395_cov_9.925079, whole genome shotgun sequence.
ACCESSION   DAWDWH010000110 DAWDWH010000000
VERSION     DAWDWH010000110.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5355_length_6395_cov_9.925079"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1426..1435
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4678..4687
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1979 a         1201 c         1657 g         1538 t
ORIGIN      
        1 tcacggaaga gcagaataaa ggagcagatg cagcaaatca gagttccaaa aagaaagacg
       61 gaaaagagca gagccaggaa acacaggaag aatcagaaaa tgctacagac agtgcggatg
      121 ctgatgtaag tgaaaatgca gccgatgaaa atggtgaaaa tccggcggaa gaaaacaacg
      181 atgccggtca ggatgccgga aatgataaaa aagaaaacgg acagaaagca gatgccaagg
      241 atccgggcgg cgatgcagct gatcaggaag atactgcatc agcttccaat acctgtacga
      301 tttccatcag ttgtgcaacg attctggaca atatggacca tttaaagagc agcaaggcag
      361 agtttgttcc gtccgatggc tggattttgg caccgacgga agtagaattt accgaaggcg
      421 aatctgtgca tgatgtactg cagcgtgtct gcaaagaagc gggtatccag atggaatcca
      481 gcttcacacc ggcctataat tctgcttatg tagaaggcat caacaatctg tatgaatttg
      541 actgcggaga actttccgga tggatgttca gcgtcaatgg ctggtatcca aactacggat
      601 gcagcaaata taccgtaaat gcaggtgatg agatctgctg ggtatatacc tgcgatctcg
      661 gaaaagatgt aggcgataat tctatgtatt agagggcaga tgccggaggt aaatatgaaa
      721 gatacatttt ctacatacca tccgcttctg aacatgcttt attttgtcgg agtgatcgga
      781 gtgacggtat ttgttgttca tccggtcatt ctggcaattt cgctgtttgg aggaattgca
      841 tattccgttt tgctgaaagg atggaaaaaa acagttaaat ttaatctgat cttttcgata
      901 ccgatgatgc tgatcgtggc actgatgaat ccgatgttca accactatgg tgtgacgatc
      961 atcggttatc tgcacaatgg caatccgttt acactggaga gttgtgtcta tggtctggtg
     1021 atggcgatga tgctggtgtg tacaatgatc tggttttcct gttatacgat cgtaatgaca
     1081 tcggacaagt ttatctatct gttcggaaaa atcataccgg cactgtccct ggtgctttcg
     1141 atgtgtctgc gttttgtccc gaaatttatc agagaggcag gtgtgatatc agatggtcag
     1201 aaatgcatcg gacgaagtac cgaaaacggc agtctggtaa agcgtgcgaa acatgggatc
     1261 acgatatttt ccattctggt aacctgggca ctggaaaatg cgatcgagac ttcagattct
     1321 atgaaatgcc gcggttacgg tgaaaaagga agaacggcat tttctctgta tcgatttgat
     1381 aaacgtgata ttctctgcct tttgtttatg ggagcaagtt tcgcannnnn nnnnngtcag
     1441 tataacccga aaattattgt aaagggaatt cctctgacag caggaagcct gctggtatat
     1501 ctggcatttt tgctgttctc cgtgcttccg gtggtgctgg aactctggga tcgatatcag
     1561 tggaaacaga gacgtaaaca ggtagaaaaa gcagagcagg ccaattatcg cctgtgggaa
     1621 gtacaggatt agaaaaagga gataggcgat gtcagaatcg atgggaaaag aaacgaaaca
     1681 aaacagcagc aaaagtgata agaagaatat taatcatatt gataatagta tagaaagcca
     1741 gacaggtaga ccaggttcga tcctggagat caaagatctc tgttttgctt atccggaaga
     1801 agaaaaaaga gcactgaacc acatttcctt tcagataaaa gaaggagaat ttctggtgct
     1861 gtgcggcaag agcggatgcg gaaaaagtac gctgttaaaa catatgaaga caccgctgac
     1921 accgcacgga aaacgaaaag gagaaattct ttttgacgga aaaaatctgc atgagatgac
     1981 aggcagggaa cagagtcaga gaatcggtta cgttctgcaa aacccggaca atcagatcgt
     2041 gacagacaaa gtgtggcatg agctggcatt tggactggag agtcttggat acaaaagtgc
     2101 agagatccgt atccgtgtgg cagaaatggc aagttatttt ggaattcagg actggtttta
     2161 taaaaatgtg gcagaattat caggcggtca gaaacagctt ctgaatctgg cttccattat
     2221 ggctatgcat ccggatattc tgattctgga tgagccgact tcacagctcg atccgatcgc
     2281 tgcgtccgat ttcctgggaa cagtaaagaa gatcaaccga gatctcggta caacgatcct
     2341 gttgacagag caccgcctgg aagaagtatt tccggcagca gaccgtgttg tcgtgatgga
     2401 gggcggagaa gtactgtgca tgggaacacc ggaagagatc ggggaagaat taaaaaaaca
     2461 gaaccacgaa atgtttctgg caatgccggt tccgatgcag atttatgcaa aagtaaattc
     2521 cggaaagcat atggagtgtc cgattacagt ccgggacgga agaaactggc tgaatgcgat
     2581 ctgcaaagaa aaacagatcc ggacgcagga ggcagaattt ttcggaaaag atgcgaaaga
     2641 aaagttggcg aaagaagaaa tcattctgga ggcgaaagat atctggttcc gttatgacaa
     2701 agaccagccg gatgttgtaa aaggactcaa tctctcactg aagcgaggag agttttttgc
     2761 attgctcgga ggaaatggaa ccggaaaatc aacgacgctt tcgatcatca gccggcttcg
     2821 aaagccatac cgcggcaaag tggtgctgga aggaaaagat atccgccgtt acagtgaaaa
     2881 ggagctgttt cgtggctttt tgggggttct gccgcagaat ccgcagtctc tgtttgtaaa
     2941 aaagacggtg gagctggaac tgttcgagat ggtgggcgga acaaaagaaa agaaaaatga
     3001 agagtatcac cttgccatgg aaaaacgcga ggcagtggaa ggaatcgtca aagtaacgca
     3061 tttagaagga ctgctgcacc gtcatccata tgatttaagc ggcggggaac agcagagact
     3121 ggcacttgca aaaattctgc tgctgcgccc gaaacttctt ctgatggacg aaccgaccaa
     3181 aggtctggac agttatttta agaaagagtt cgcagaaatt ctggatcttc tgaaaaagca
     3241 gggtgtaacg atctttatga tctcacatga tatcgagttc tgtgcttctt atgcggaccg
     3301 ctgcggtctg ttctttgacg gaaatattgc ggcgagcggt acaccaaaat ctttctttgc
     3361 cggaaataac ttctatacga cagcggcaaa caggatggca cgtaaatatt ttccgatggc
     3421 agtgacggca gcagatgttg cggaagctct tttggatgac agcaggtgtg tgtgatatgc
     3481 aaaaaaagat gaaaaatgag aagatacagg gagtgcaggc cgggaatatg gaaagagaac
     3541 aggaaacaaa agaatttctg aagcagtttt atgaatcgcc ggaagcagag gaagatacga
     3601 tcgcagcgaa gctgaaacgc accttcgtgc caaaaggcaa aaagacaaaa gcaatcgcag
     3661 attttgccaa agatctgatg ggcgaaggcg gagccggtga tgttgtgatc gatgatgata
     3721 tgtttgaggc agacaccgaa ggctataaac aacaggaaaa gaaacgcctg tcaaaacgga
     3781 ccagagtcag tctgtttttg cttctggtgg caatccctct taccattgca gccggattct
     3841 ggctgacaga tcaaagtgca ctggcagact ggatcgatcg agtgtttggt ccgagaaaat
     3901 attatctgat cagcctggtg atcgtgcttt atgcattgct gccgtttttt atggcatttg
     3961 aaggaagaaa accggaagtc agagaactgg ttgtgttggc aaccatgatc gcactggcca
     4021 ctgccggaag agcggctttc tttatgacgc cgagctttaa gccgatcatc gcgatcgtga
     4081 tcatatcggc aatcgccttt ggagcagagg ctggcttttt ggttggatcg atgaccatgc
     4141 tggtcagcaa cttcctgttc ggtcagggcc cctggacgcc atggcagatg tttgccatgg
     4201 gatttatcgg atttttatcc ggtatcctct accgactgcg gatcctgccg acaaaacggc
     4261 tgcagctgtc cgtatatggc tttctggtca cctttatgat ctacggcggg atcatgaacc
     4321 cggcggcact gtttatgagc gtatatcaat tcagctggag cggactgctt gcgatctata
     4381 tctccggtgt tccggtcgat ctggtgcatg caagctcaac cttcttgttt ttgtggatcg
     4441 gggcgaagcc gctgctggag aaactgcaga gaatcaaggt gaagtatggg ttgttgtagg
     4501 aaaatcgaaa aactcgcaat gggagcttta ctaccatgtt ttacagttct gaaaaagtgt
     4561 caaaaaaatg tacaaattcc ataatttgct aaaaaaacac acaaatctgc aaaaatgtac
     4621 aaaaaatgca caaattcgac aagttgtcta ttttgaaaat tcattctgag cgatacannn
     4681 nnnnnnngca aaggttgaaa gaaaaataaa aagaaccttg cagaaagctt atatggatgg
     4741 aggaaaaatg tatgtcattt gcagatttta aagacaacac catgagagcc gttgtatcaa
     4801 aaacgattga tggtgtattg aaatacgtga ataaacatga tgatcgaaca gaggcattac
     4861 tcaaactgac tgatctttcg gaaaagctga tgggcgataa atttgagaaa aaagtatttg
     4921 acggagcaag aaatctgatc cataatccag atgcaaaatg gatgaaatac acaaacaaag
     4981 tgcttgatga gattgacccg catgtagcga aaatgcatgc cctgaacctt ggatatcagg
     5041 caggttttta tggttattcc aaaacctgtg aattcgagaa ggaacatgga taccgcatcc
     5101 cgtggattat tttgatggat ccgacctctg cctgtaatat gcactgtacc ggatgctggg
     5161 cagcggaata tggtcatcgc atgagccttt cttacgaaga tctggacagc atcgttacgc
     5221 agggggaaaa gctgggtatc tatttttaca tgatgacagg cggggagccg ctgatgagga
     5281 aaaaagatat cgtgaaactg gcagagaagc ataataagtg tatgttctat gcatttacaa
     5341 acggaacgct gattgatgag gaattctgta aggatatgca gagacttgga aatatttctc
     5401 tggcactgag cgtggaagga tttgaggaag tcaacgattc cagacgcgga agcggatgtt
     5461 ttgagaaagt tatgcatgca atggatctgc tcaaagagca tggactgatc tttggtactt
     5521 ctatctgcta cacatcaaag aactacaaga ccgttaccag cgatgaattc ctggatatgc
     5581 tgattgacaa aggtgttcgc tatgcatggt atttccatta tatgccggtc ggaaatgaag
     5641 cagcaacaga tctgcttctg acaccggagc agagagaata tatgtaccac agaatccgtg
     5701 agatccgagg atttgaagga ggcaagccaa tctttgtatt tgacttccag aatgacggtg
     5761 agtttgtcgg cggatgtatc gccggaggaa gattctactg tcacatcaat ccaaacggtg
     5821 acgtagaacc ttgcgtattt atccattatt ccaatgcaaa tatccatgac aaaccgcttc
     5881 tggaatgctt aagccagccg ctgtttcgtg cttatcagat gggccagccg tttaatgaaa
     5941 atgatctgcg tccatgtccg atgctggaga atccggaagc actgcagaag atcgtccatg
     6001 caaccgatgc aaagagcacc gatatgcaga gtccggaaac ggtagagagc ttatgcggaa
     6061 aatgtgaagc ttacgcaaaa tgctgggcac cggaagcaga aaaattaaaa gaagcaaatc
     6121 caaagcagat caccagagaa aaaggtacac tgccggaagt ataaaaaaac agaagttgat
     6181 atatcagaaa aaaggatcgt cagaagcagc ttcattcaaa cttccgtcct cctgtccagc
     6241 aagtgtacat aaaatttcca aaaatgaaga taatgattac tgttcataaa agcctgaatc
     6301 aggtttgagg tgacaaatat catgtatgac aggaggaact tatggagaca gagatcaaaa
     6361 tgttgagtga cattcacaaa aatgcagaca tggga
//
LOCUS       DAWDWH010000111         6391 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5358_length_6391_cov_15.781250, whole genome shotgun sequence.
ACCESSION   DAWDWH010000111 DAWDWH010000000
VERSION     DAWDWH010000111.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6391)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5358_length_6391_cov_15.781250"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1659 a         1561 c         1337 g         1834 t
ORIGIN      
        1 tgcttctata tatacgccta aaatccatga aaacctggct ggctttgctg gactttttct
       61 tttaaaagtg atattatatc gcgtaagcaa ttagcttacc ggcatgctct ttatgctggc
      121 attttcgcta tttataatta tggaggtgaa tcgctatgtt gaggacaata ttgaaaccac
      181 tttctttcgt acctgccctc attatgatgt atattatctt ctccttctcc gctcaggacg
      241 gagatatgtc cggtcagctc agctaccagg caagtgtgaa gatcatcgaa acagctgact
      301 atgtttttaa cgccaatctc agttacgatc aaatcgatga gtgggcaaat aaaatcgatt
      361 ttatcacccg caaactggca catatgaccg aatatttcgc tcttgccatt gcagtatcct
      421 ttccattata tgtatatgga ctgcacggca tccttctgat gtttctggcg gggctgatct
      481 gcgtcgggtt cgcctgcgga gatgaattcc accaatcgtt tatcgccgga cgaagtcctt
      541 cggcaagaga tgtctgtatc gacagtgtcg gcgtgttttt tgggatcatc gtcgtccgca
      601 tcatcggctg gaccggacgg aagacggtct ttaaagaaaa agtcaaagta aaacagaaaa
      661 agccaaagaa aaagctcttt ggcggtaagt caaaaacaac ttactatgat aaatacggca
      721 attaccagcc accacagccg gatgaccgct attaccagaa tccaggatct ggatatggtt
      781 atgacaaccg tgggcaggat tatggttatg atagtcgcta tgacagccgc agcgggcgta
      841 attattacga acagccgggc ggcaatccga attatggtta cgataaccgc ggcggccgcg
      901 attattatgg tcagccggaa ggtaacccaa attacggcta tgacaaccgc ggcggtaatc
      961 cgaattatgg ttatgacggt cgcagcagca atccgaatta tggctatgat aaccgcagcg
     1021 tcaatccgaa ttatggttat gataaccgca gcggcaatcc gaattatggt tatgataatc
     1081 atggacatcg tgattattat gaacagagcg gcaaaaatcc ggaaaccggt tacggatacg
     1141 acaactgcga gcagaattac tgcgaatcac agactcctga ccgcagtaac cgcgattact
     1201 atgagcagcc cgtcagggag tcgcagtcac agcagagtac cagaacctgg tcaagggaag
     1261 aagtagaagc tggacttcat ccggagcata catcacgtac ttccgacaaa aaggctacta
     1321 atccaaacga tcctttctgg tctccggaac aggaaatcga cttcaatgcc aaaccggaat
     1381 atgccgagcg aaattatcag gaaacaaatg atccatatga tcatgactgg ggatttgacc
     1441 gttccgaaaa tgtttcccaa cagccatctt attcaccaga aaaagaacag gctccatatg
     1501 agaatccgga aagtaccgtt ccagacgaac ctgcggaaga tccaagacag actcctccgg
     1561 tacagcaccg aagaaaaaag aaacatgaaa aagactggtt ctttgatctg taaaggtttg
     1621 ttgctcacca cttgcagaag tgggagtctt ctcgactgag ataaaatcgg aaattgagtt
     1681 tcgattaaaa ctaaacgaaa aaatgcatgt actttcgtca tccgacaaaa atacttgcat
     1741 tttttgtttt atcatttttc gttttttgct tattattctg ttccctgtta ctctgttttt
     1801 tcgctgcttt cttcctgctt ctgtctctct tcttccttct cctgctgttc tttcacaaat
     1861 tgctgtaatt cctgtttgtg ctcttcgctg agaccatgat cgccctcaag agaacgtaaa
     1921 aacaccagca gagaaccatc ataggcaaga ttcaggtatt cctgaacacc ttttgctata
     1981 aattcttttc ttgacatgtt cacagaataa tagcgacgtt tacctctttt ggtagaagac
     2041 aggattttct tattcttcag acgtatcaca aaactatagg ccgtctgctg tttccataca
     2101 tatccttttg tctccatgta ctccatgatt tcattcagat gaatctcctc ctgatctgca
     2161 tgtgcccaca tgtagtccat aatgtgctgt tccgcttctg ataaactgta aaaagccata
     2221 ctttttcctc ctaatcgttt gtaatataat acaatgctat attacatatc ttatcaaaaa
     2281 atcgccagat ggtcaaatca aaaattcgag aaattttgtt ccgttttgtg atcatttaca
     2341 gaaaataatg catgaaacag aagaggccta cagtcgagaa tcatctcacc tctgatatag
     2401 tatgattgca aacaccgctg tcaatgcctc tgccaccgga aatgccagcc atacgccgtt
     2461 tgctcccagt atttttccaa acagaaatgc tgccggaata atgacaatga catatcgcag
     2521 caacgcaata caaaaagatg gtgtaccctt tccgagtcct tccagtgctc cggagcaggt
     2581 cacagacacg ccggacacga taaagccaaa gctgatgata tgaagtgcag tgattccgat
     2641 ccggatggtc tcttcattca cggtgaacag cccgatcaat ttttccggga tgagccagga
     2701 tatcagcatt ccggctgcca tcacaccaag caccagtccc agtgccgtcc ggaatatttt
     2761 ttctacccgt tttttctctc ccgctccata attgtagccg atcagcggac ggatgccctg
     2821 gatgatcccg tttgcactca gataaataaa ggtctgaagt ttataatata cgccaagcac
     2881 cagcacatat ttctccgaga atcctgacag gataccattt aaagatgaaa tcagcagaga
     2941 cggaagtgcc atatttaaag tcgccggaat gccgacagta taaatacggg aaattacctg
     3001 tttctccggt tttaaatatt ttaactggat cttgaccggg atcgggtctg caaaataaaa
     3061 gatgagatag atcagcagtg tcgccgtctg tccgattccg gtcgcatagg cggcaccggc
     3121 aatccccata gccggaaatg ggccgattcc aaagatcatc agcggatcga gcagaatgtt
     3181 gatcagacac ccacagatca tgctgaacat ggaaactttc attttaccca ccgactgaaa
     3241 gattttttca aaggttatcc ccagtgtgat cacaacggag aagagcagca cacgatttcc
     3301 gtatgtgatt gccatcaggt tgacttccaa atcagcggaa aatcctttca gaaacttcca
     3361 cagacctgct gtacacagga ctgtcagcaa tattccatgc aaaacactta aggcaatgcc
     3421 ctgcgttgct atctgatttg ctgtggtctg tttttttgct cccagataat aagcgatccc
     3481 ggcattgatc ccgattccaa taccaacacc gatggctgta atcagattct gtatcggata
     3541 tacaagtgcc agtgccgtca tcgcttcttc acttaatttt gcaacaaaat agctgtctac
     3601 gatgttatac agtgcattca ctgccatgga gatgaccatc ggcagcgaca tggataacac
     3661 cagcggcagt atttttcgct ctttcataaa actttgatcc atatatttcc tccatctaat
     3721 aatacaacgt gctttacaga ctttatcagc tgattagcaa tcgccaaata ccagcatgct
     3781 gtatttttct caacatggca acaaaaaaac gccacataac tacctttcga tagctatgtg
     3841 gcgaaaaaag aactttctta aaaaatccac accctgtacg ggttttgtta ttctctttat
     3901 tcttcggtag aatccgaatc ttccgctgtc aaatcgactt catcgaaatc gtctgcaaca
     3961 ttctcatctg cattctctgc tgcggcagtt tctgcatctg ctgttgtttc cttcgaagtg
     4021 atctctgtct gctcttccga catttctgtt tcatccagca tcagatcgtc atcttccatt
     4081 tcatattcat cctcaccggt atccagtttt acgttgcggt aacgtttcat accggttccg
     4141 gccgggatca gtttaccgat gataacgttc tctttcagac cgatcagcgg atcgacttta
     4201 cctttgatag ctgcttctgt cagaactttt gtagtttcct ggaaggatgc tgctgacagg
     4261 aaggagttgg ttgccaggga tgcttttgta ataccaagca ttacctgttt tccctctgca
     4321 ggctctttgc cctgagcgat catctcttca ttgatatcct cgaaatcaag gatgtctacc
     4381 atggttcccg gcagaagttc cgtgtctccg ttatcctcta tacggatctt cttcatcatc
     4441 tgacgaacga ttacttcgat atgtttatcg ttgatctcta caccctgcag acggtataca
     4501 cgctgtactt cacgcagcat gtaatcctga acagcgttca cgcccttgat tcgcagaatg
     4561 tcatgcggat ttacactacc ttctgtcagt tcatcaccag catccagata aactccatcc
     4621 tgtactttga tacgtgatcc gtaagggatc agataagtct tggactcacc tgtctcactg
     4681 tttgtcacaa taatctcacg ttttttcttg gtatctttga gagttgccac accggaaatc
     4741 tcagtgatga tagcaagtcc cttaggctta cgtgcctcga aaagctcctc gacacgagga
     4801 agaccctgtg tgatatcgcc gccggcaaca ccaccgttat ggaaggtacg catggtcagc
     4861 tgtgtacccg gctcaccgat agactgtgct gcgatgatac ctacggattc accaacctgt
     4921 accggctcac cggttgccat gttggaaccg taacatttcg cacaaacacc aacatgtgat
     4981 ttacagttca ggatcgtacg gatcttgatc tgttcaaatg gtttgccgtt tgcgtccaca
     5041 ccttcactca tgatacgtgc tgcacgtttc ggagtgatca tatggtttgc ttttacaagg
     5101 atttcgccgt ctttattctt aatggtctcg caggagaatc ttcctgtgat acgctcctgc
     5161 aggctttcga tctcttcctt gccgtctgta aatgctttta catacatacc cggaatttct
     5221 ttgccttcgc agcagtccat ctcacggatg atcagatcct gtgatacatc aaccagtcgt
     5281 cttgtcaggt aaccggaatc ggctgtacga agagcggtat cggacatacc ttttcgagct
     5341 ccatgtgcag acatgaaata ttccagtacg tcaagacctt cacggaagtt tgacttgata
     5401 ggcaactcga tcgtatgacc ggttgtatct gccatcagtc ctcgcatacc tgccagctgt
     5461 ttgatctgtt tatcagaacc acgggcaccg gagtcagcca tcatgaagat gttgttgtac
     5521 ttatccagtc cgtccagcag ctttctggtc agaacatcat cggtttcttt ccaggtctct
     5581 acaacttctt tgtaacgttc ttcttctgtg atcagaccac gtttgtagtt ctttgtgatg
     5641 tggtctacag tctcctgagc ctgcttgatc agttccggtt tttcaggcgg aacggtcata
     5701 tcggaaatgg atacggtcat agctgctctt gtggagaatt tatatcctgt tgctttgata
     5761 tcatccagca cttctgctgt cttggtagct ccgtgtgtat tgataacttt ttccaggatc
     5821 tgtttcagct gtttctttcc tacatggaaa tctacttcca gcttcatttc gttttccgga
     5881 atgcttctgt ttacaaagcc cagatcctga ggcaggatct cgttgaacag gaaacgtccc
     5941 agtgtggact ctaccgtacc ggtctttgtt gtaccgtccg gcatggtctt tgtcatacgc
     6001 actttgattc tggtctgcag agtcagtgct ttgttttcat aagcaaggat tgcttcgttc
     6061 acgctcttga agtacttgcc ttctccgatc gaacccggtc tttcctgtgt caggtagtaa
     6121 ataccaagga ccatatcctg tgaaggaacg gctacaggac cgccgtcgga cggtttcagc
     6181 aggttgttcg gtgagagaag caggaaacgg cactctgcct gagcctctac ggacagcggc
     6241 agatgtacag ccatctggtc tccgtcgaag tcggcgttga atgcggtaca aaccagcgga
     6301 tgcagtttga tcgctttacc ttctaccagg atcggctcaa atgcctgaat acccagacga
     6361 tgcagtgtag gagcacggtt cagcataacc g
//
LOCUS       DAWDWH010000112         6346 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5401_length_6346_cov_14.422667, whole genome shotgun sequence.
ACCESSION   DAWDWH010000112 DAWDWH010000000
VERSION     DAWDWH010000112.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6346)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6346)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6346
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5401_length_6346_cov_14.422667"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6024..6033
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2195 a         1295 c         1512 g         1334 t
ORIGIN      
        1 catccaccta tgagcaagct catgtggatt tagacctttt gaccctggca tcatgaaata
       61 agtgaataga aaaaggaggc acggcatata tgaagagaac ttcaaaacgc gtgatggcag
      121 tcgcagtgtc gcttgcaatg gtacttaccg gcagcacaag tgcactggca gcaacaacga
      181 acccggaaat cagtgaccga gagatcgaac ataaaacagc ggcgaaaaac attgcagcac
      241 agggtatggt ccttctggaa aacaagaaca attccctgcc gatctccaaa gaaaaaggaa
      301 ctaagatcgc actttttggt cagggcgtat acaataccat caaaggaggt accggttccg
      361 gtgcagtaaa ccagcgtgat aacgttaccg tacagcaggg ctttgaaaat gcaggctatg
      421 atatcgttga tattgacttt gtaaatcaga tgcatgaaag atggacggca aatggcggcg
      481 gaacaagcca gggctggagc tacaaatggg taaatgaacc gatttatgag cagaccgaag
      541 gtgccgtaga tcagatcaaa gcagcagcag aaaaaacaga caccgctatt tacgtgatcg
      601 cacgtaactc cggtgaaggt tctgaccgtt ccgcaggaaa aggtgattac ctcttatccg
      661 acgatgaaga ggcgaactta aagctgctgg gacagaactt tgaaaatgtt gttgttgtcc
      721 tgaacgtagg cggaatcatt gatacaaagt tttttgatga gattgatggt ctggattcca
      781 tgcttctgat gtctcaggcc ggtatgacag gcggagatgc gttggtagaa gtattgaacg
      841 gaaccgtaaa tccatccggt aaactgaccg acacctggcc ggtgaatttc gatgacaacc
      901 catcgagtga aggatttgca aaccaggacg gcaacaccgc tcaggaatta tataacgatg
      961 atattttcgt aggatatcgt tattatgata cgtttggtgt ggatgtctct tatccgtttg
     1021 gttacggtgg atcttacaca agttttgcga tgtcaacaaa atcggtaaaa gcagacaaag
     1081 ataaagtaac cgtacaggtt aaggtaaaaa atactggtat cgtatccggt aaagaagtag
     1141 cagaagttta tttctcagct ccggacggtg aactggacaa gccatatcag gaactggccg
     1201 gctacgccaa gacagacgaa ctggcaccgg gcgagtttca gatccttacg atcagttttg
     1261 atacgaaaga aatgggatct tacgataccg acaaagcagc ttacgtgatg gaagatggcg
     1321 attacattat ccgtgtcggt aattcttcca gaaatacaca cgtagcagcg aaactcaatg
     1381 tcgcagaaga cacgatcacc gagcagtaca gcaacctgat ggttccggta acagaaaacg
     1441 acaacatcga aaacagtgat aaatatccga ccctggagcc gatgacaacc gaaggtgcaa
     1501 catcgatcac atacgatggc gaagcaaatg agattgcggc agcgaagacg attgaagtgg
     1561 attttacagg ctacaaagca ccggagatca tcaaagagaa cgaagatgta acggtttaca
     1621 cttccgatac gacagaagaa aaatatctgt ttgccaagaa tgaagacgga gcaacaggaa
     1681 ccgtttcccg tgtagaaaaa ggaaatgcaa acagcacaga ttacagtgtg acttatcagg
     1741 agcacgttaa gaaatttgac ggcgactaca gcaagaatac actgaaagat gtttacgacg
     1801 gtaagatcag tcttgagcag ttcgtatccg gtttaagcct ctatgagttg gcaaacctgg
     1861 taaacggaca ttccagtgac aagaccgtac agggcgttgc aggagcaacc tggaaaaacg
     1921 atgaaaaagg gtttgtacct gtaaacctgt ctgacggacc tgcaggtctt cgtattacac
     1981 agcactataa acaggatgat accgactatt atcagtatgc aactgcatgg ccgatcggta
     2041 cactgctggc acagacctgg gataccggac agatctacgc ttacggcgaa ggcgttggcg
     2101 aagaaatgga agaatttggt atcggttcct ggctggcacc tggtatgaat atccacagaa
     2161 acgcactctg cggacgtaac tttgaatatt attctgaaga tccggtagtc gtaggtgtga
     2221 ccggtacagc agcaacactc ggcgtacaga agagcaaagg tgttggcgtt accatcaaac
     2281 actacgcact gaattctcag gaaacaagca gaaacagcga gaataatacc gtttctgaaa
     2341 gagcaatccg tgagatctac ctcaaaggtt tcgaaatggt tgtaaaacag gcacagccga
     2401 tggcgattat gacctcctac aaccagaata acggaagacc ggcagcggat gactatgatc
     2461 tttgtaccgc atttgcaaga gacgaatggg gattcaaagg catgatcatg acagactggg
     2521 gcggcggaca gtctgtaccg atgtacgaaa tgcacgcagg caacgacctt gtttgcccgg
     2581 gcaaaggcta ctctcagatc atgaaaggct ttatcaatga gccggcatgg acaagcgatg
     2641 gttatgtaga actggaagag agaagcattc aggatgttga tgcaagcgga aatccgatca
     2701 cagtttctaa gatggttccg aactttggcg gatacaaact ggatctgaac ggcaatctgg
     2761 aaatctccac aaccgtagca aaaggcgtag aactgaacga aaaagttgca gaactgaaag
     2821 aacagggtta tatcacatct gttacaacaa ataggcgtgg tgtgacaact gttacttata
     2881 aagtgagcat ggatgacggc gtagaatcta cacaggttat ctctcttggt gaccttcaga
     2941 aagcagccat caacatctgt aactttatta tgaattccat cgaatttgca aacgcaaacg
     3001 acctggaagc aaaatcactg aacgaactgt atgccgacgc actgcagacg atcacttcct
     3061 atgaaaaaga tgacgttgtt ccgtccacaa catactccac gatcaccgaa ggttatgact
     3121 ggggtccggc aatctccaag gttgttgtaa atgtcggcaa agcaatgaga ggaaatattg
     3181 acgcatctgc atttgaagta aacgtaacaa gaaaagcact cagcggattc cttcttggac
     3241 catccagagg aacacgtaac gtaaccgcag cttatgtatc cgatgcaaac ggaaacaaag
     3301 cagcaacggg cacttacatt actctggaac tggaagtagg accggatctg tccatcggat
     3361 ctccgttcaa ctacaacttc ttcggaagca gccataatga gtatgtagac actgcatatg
     3421 aagtaacctt aaataaagaa ctcaaagcag cagacggcag cagcgtaaat tttgcagcaa
     3481 ataaatttgc agcagacacc acacggatct gtgatgactt tgatcttgaa ggtaaattca
     3541 gctacaacga tgaaaaattt ggtcagatcg ctctgacata cgcttcctat acaccggaag
     3601 cagctaagaa tgacggcaag aaaaatgctc tgatcatctg gctgcatggt gcaggcgaag
     3661 gcggaacaga tccacgtgta gcactggtag gaaacaaagt agtcaacctg gcaaccgata
     3721 ctgtacagca gtactttgac ggcggagcat acgttctggc agcacagtgc ccgacgatgt
     3781 ggatggatga tggttccgga cagtatacaa gcgatggaac ctccaaatac acagcagctc
     3841 tgatggaact gatcaaagct tatgtaaaca gcaactctga tatcgatgca agccgcgtat
     3901 acatcggcgg atgctcaaac ggcggattta tgaccatgaa catgatcgta cattatccga
     3961 aatactttgc agcagcatat ccggtatgcg aagcttacac aaacgatgca ctgacagatg
     4021 aaatggtaga atctatcaaa gatatgccga tctggttcac acatgcaaag aatgacccga
     4081 ccgtaaaaat cggtaccatt gatgaagacg gcaatttcgt atctaacggt aactactcac
     4141 cgaaggctta cgagagactg acagaagcag gcggaagcga tatccacttc tcactgtttg
     4201 acgatgtaca cgacaccaca ggtctctaca agagagcaga cggcacaccg tatcagtaca
     4261 acggacactg gtcatggctc tacactctga acaacgagtg caaagaaaac atcgacggcg
     4321 aagaagtaac catctggcag tggatctcca caaaatcaaa aacaaacaga ggacttgaaa
     4381 aagtcatcgc aaaagtaaac gcactgaacg cagaagacta cacagcagac agctgggcag
     4441 cagtacagag tgcactggca gcagccaaag cagtagcagc agatcagaat gcaacacgca
     4501 cacagatcaa cgcagcaatg gaagagctgg tagcagcata ctccaaatta gagtatggcg
     4561 tacagaaact tcatctggaa gttgcagtag aagaagcaga aaagatcctg gcacatccgg
     4621 aaaattatga agacattgca gctctgaaag cagcactgga aagcggaaaa gcagttctgg
     4681 aagataaaga tgcagatcag acagcagtag atgcagcagc agaagcaatc cttgcagaac
     4741 tgaacaaagt agcagacaaa gcagatgtga aatctctggt taatctgatt gaatccgcaa
     4801 aaaaacttct ggacggcaat tacacaagcg attccctgaa agacctcaaa gcagcgctca
     4861 agaaagcaga agatgttata gcaaatccga acagaacaga agcagagatc ggaaccgcat
     4921 atgcagatct gatcaatgca atcctgaatc tgcagatgaa agctaacaaa gcagctctga
     4981 aagcaatcct tgacaaagca aatgaagtac ttggaaacgc agacgcttat gtagcagcaa
     5041 ccatcaacgg actggatgca gcggcagcag aagcacagac agtatacgac aacatagacg
     5101 ctcttcagga tgatgtagac gcagcagttc gtgcactgac acgcaaagtg gcagatgcaa
     5161 gacttctggg cgacgtagac aacgatggtg aagtaacaac agccgacagt gtagcacttc
     5221 tggcagcaag tgcagaaatt acaagcctcg atgcagatgc agcagcagcc gcagatgtaa
     5281 acggagacga cgtggcggat acaaacgacg cagttctgat ccttcagtat gcagcagaaa
     5341 aagttgcatc tttctaagcg gatgaagaac ctgcaaaaat gaatcacaaa aagagatgcc
     5401 acagatcaca gatcctgaca tttcgataaa aacggagcgg tgtagcatta agttacaccg
     5461 ctccgattgc gtttcatgac tttgatcaaa tataaaatag ctgaaatttc atcttccgtc
     5521 agtccggaga aatcaactat aatggtatcg taatttaaga cacttcaata gacatttctt
     5581 aatgaatctg atatactgta aacactacag aaagaaggat tatagtgtga aagaaagcag
     5641 aggacagccc taaatgggca cacttgagat gagtatcaga aaaaaagtaa aatgaataca
     5701 tcaagtgttt ctgaaaaaaa tgggaaccgt gaaaaataag caataacaag gaaaaaggag
     5761 agcgaaagaa atgaagaaaa tcaaaaaaat catgatgacg ttaacaatgg tggcatttat
     5821 gtttggattg gcaaatattg gatcagtgaa agtacaagct gccagtccgt ctatagacaa
     5881 gagtgtaact tataatttat ataaaggtca agtggggttt ggtagtgtct tgattcataa
     5941 tcctgttaca aatgcaaaaa taacaggttt aaagaattcg aattcaaaag taatagaagt
     6001 tacagcaagt gaagacgaca tgtnnnnnnn nnncatgtat ccacatttag aaataaaact
     6061 ggtcggagaa ggaacaacaa aaatttcatt tcagtatgcc ggaaaaactc tgacgcagaa
     6121 aattacggta aggaaataca aaaatccgtg taagttattg aaaattggaa agacgaatta
     6181 cgctaaatgt tttaataaat caggacatta caaccataat aagcgaacaa aaaacgttac
     6241 cggaaagata accataaaac cgaaaaaggg atggaaatta aagaaaatag aagtattcaa
     6301 tatattcgat ggtgtaaaaa aggtgaagaa taattcaaaa gtaacg
//
LOCUS       DAWDWH010000113         6309 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5432_length_6309_cov_11.443556, whole genome shotgun sequence.
ACCESSION   DAWDWH010000113 DAWDWH010000000
VERSION     DAWDWH010000113.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6309)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6309)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6309
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5432_length_6309_cov_11.443556"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1555 a         1717 c         1283 g         1754 t
ORIGIN      
        1 ttgcaccttt ctcacgcaga aatctgcaaa gttctgcaat ttccggttct tttgcacagt
       61 tttcaatccg ggtacgccct ctggcataaa cagccgcaag aatgacattt tccgtggcac
      121 cgacacttgg aaaacgaaga tggatcctgc tcccgcaaag gccgtctgtc actgcacaga
      181 gcatgccgtc tgtctcttca aagacagctc ccatctttcc aagggaggtg atatggatgt
      241 cgatcggccg catcccgatg atacagcctc ccggaaacgg cattttcgcc ctcccggctc
      301 ttccaagcat cgatccgagc agaatgatcg tggaacgcat ccgctcgccg agcgatgctg
      361 cgacctcaaa atgttctgct tttgcagcat ctatgatcag cgtcccgttg tctttctgta
      421 ccgtacagcc gatattctcc agtatttcaa tcatcacatc aacatcacga atcgccggaa
      481 catgatgcag cgtcgtcctg cccggatgca gaatggatgc cgccatcaaa ggcagggccg
      541 catttttgga accctgaagt gtgatacttc cgtgcagagg tgttccacct gttatttcaa
      601 agtattccac ccaaacccct ccgcttactc aatctggaaa ttgcttctat aaaattatca
      661 tatgcatttt ttctcgcttt gccaccggct gactgccaaa accagcccca tctccgagag
      721 cagaaacaaa acggaagtac ctccataact gataaacgga agtgtgatcc cggtattcgg
      781 aatcgtgttt gtcacaacgg ctacatttaa aatcacctgg atcgcgatgt gccccatggc
      841 tcccgccgcg atcagtgacc cgaaaagatc cggtgcatgt acagcaatca ccataaacct
      901 ccagatcagc accagaaaaa caagcatcag gatcgtggca ccgacaaatc caagttcttc
      961 gcagatgatt gaaaagatca tatcattctg tgcctccggc acgaatccga gcttctggat
     1021 gctctcgccg aatcctctgc cgaacagtcc gccggaaccg atcgcataaa gtccctggat
     1081 tgtctgatag cccttttcgt aagcctccgg attcttccag atggcgatcc tctccaggcg
     1141 gtaagactcc atcccaagga acaacgccat catgcctgct ccggccgccg cgatccacac
     1201 aaactgcata tatcttttgt ccgatacaaa cactacgatc actgcgatgc cgagaatgat
     1261 gatcgccgta cttaagttgt tcgtccctac cagtgccacg atcggcagta ccagagcaat
     1321 cacaaacacc aggttcagaa aactgttcct ttttttcttc tgtcttccga ccattcttgc
     1381 cagaaacaga atcacggcaa gtttggcaaa ctcggacggc tgaaaggaaa gaggaccaag
     1441 tgagagccat cttctggaac cgttgtaagc attgcccgca aataatacca aggtggataa
     1501 tgcaagggag atcagatagg ctgcgatggc atatttttca aaaatatggt aatccataca
     1561 gctgatcagg tacatggcaa gcatcccgag tgccgctgca aaaaactgct ttttcatata
     1621 ataaaaagca tctccgaagc ggacactccc attgtaggaa ccggtgctgt ataacactgc
     1681 tagcccaaaa acaacaagaa acagcaccat gcataccagc agtacatctc ctctgccgga
     1741 ttttgttctc attgcatcac tccctgcatt cccggattat aaatttctta ctaattcttt
     1801 gaatcttctt ccgcggactt cgtagcttgg gaacatatcc cagctcgcac atgccggtga
     1861 aagaagtacg gcatcgccgc tttctgcatt ttctgcacaa actttgacgg cttcttcgag
     1921 gctttctgtc atgatgatgt tatcaaatcc acacttacgt gctgtttctg caattttttc
     1981 ttttgtaaca ccgagcagta ccagatattt tactttgccg tcaaaagact gaatccattc
     2041 cgtgtaatcg gagcctttgt catatccgcc tccgatcagc agagtcgggc gtgacatcgc
     2101 ctggatgccc ttgatcgccg catccggatt ggttcctttg gaatcgttgt aataaacaac
     2161 accgcttttc tctgtcacaa actcaatgcg gtgttctact gcctggaatg cttttactgc
     2221 ttcctggatc ttttcaagcg gaacgccgta agataatgcc atgccggctg ctgccattac
     2281 gttttcgtag ttatgtttgc cgacgatatt taaatcctgt gttttgcaga ttgcaagttt
     2341 ctgactctca tctgcatacc agatcgtctc atcttccaga taaagtccct gtttcagttt
     2401 acgctcactg ctgaaaaata ataccctgat ctgaagattt tctccaaatg cacgcaggat
     2461 cggatcttcg tagttgagga cacaggtatc ttcggatgtc tgatttttgg tgatgagctc
     2521 tttgactctt atgtactctt ccatggtatg atggcggttc agatgatccg gtgtgatatt
     2581 taagatcgca cttacctttg gatggaactg atcgatcgtc tccagctgaa aactgctgat
     2641 ctccgcaacg gtcacactct cttctgtcat cttcggagct gcctgcgtat aaggatttcc
     2701 aatgtttcca acgacataaa cgctctcttt ccagcatttc atgatttcac ctaaaaggct
     2761 ggttgttgtc gtctttccgt tcgtgccggt gatcgccaga acatcgcctt ttgcgttttc
     2821 atatgccagc tctacttcac cccagatgcg gatgccggct gctttcattt tttctaccac
     2881 cggcagatct gtcggtacac caggacttaa taccacaaga tccagctctt ttaactgctc
     2941 atcactgatc tgcccgatca ggatctgtgc cttgcttgcg gaaggcagtt tcatgcggat
     3001 tgcttcctcg tgaagctttt cattgccatc aaacaaaatt acagttgcac cgcttttttc
     3061 cagaagctca caggctccga tgccgctgat gccgcttccg aataccagga cctttttatc
     3121 tttaaaatcc atcttctatt ctcctcctcg atgttcgtat cttatactat gctgtcagaa
     3181 ttaaaaacct gaagctatga tgtcttgcta tggcttaata ctccaccttg atatctgttt
     3241 atgataccag aacgagtccc agcaagcaca acagtgctgt cacaacggta aagactgcca
     3301 caactctggt ctcagaccag ccacaaagct caaaatgatg atggatcggg gccatcttaa
     3361 agaatcgttt tccgccggtc ttcttaaagt aggtgacctg gatcataaca gagagtacct
     3421 ccaccagata gatcagtccg acaaccagga tgatccacgg catcttcagc acataggctg
     3481 cacctgccac aaatcctcca agtgccagag atccggtatc tcccataaat acctgtgccg
     3541 gatagacgtt aaataacaga aatccgagca gtgcaccggt aaccgctgcc gtgattggct
     3601 caatgccgcc gccaagcccg atggatacag cagtaaagaa tacagcgacc attgtcgtaa
     3661 cgcctgttgc cagtccgtca agtccatcgg taaagttgac tccgttgact gttccgatca
     3721 ctgccagata aaccagcgga accgtgatcc atttgcagtc aaaatactga tcgctgaacg
     3781 ggatcctcat cgtcaggaaa cttttgtcca cgatcaggat atacgccaga aaaatggtcg
     3841 taacaatgat ctgtccgagc attttctgct tcggaagcag accgtcggat cttcgaagca
     3901 caactttcag ataatcatcc agaaatccga tgataccaaa acccagtgtc agaaacagaa
     3961 ccgggatgat gttcggatat ctgcggataa agaaaagaga ggtgaccagc acactgatca
     4021 gaatgatcag tcctcccata gtcggcgtgc ctgctttttc aagatgggac tgtacacctt
     4081 cggttcgttc cgtctgaccg atcttcagtc ttttcaggaa tgggataatg atcggactca
     4141 agacgacact gattgcaaag gatgccagca atgcaatgat tacttctgta cttattttca
     4201 tatttacctc ctgctgccgc ataacggcgg cctcgttttc tggttgtcag gccgcttgtc
     4261 tgcgacgcat aactctatgc tagtataaaa cttttcctct tttttcgcaa ctgttttatc
     4321 ttagtcggaa aagactcccc ttctgtttta ttcagcagac agcaaatgtt cgacttgatc
     4381 cattgttctg accgcatccg tttcctgtgc agcttctttt tcgattccga gataaggcag
     4441 aatattttca aaaaccgtcc gcatgaccgg ggcacaaatc gttcctccat aatatacgcc
     4501 ctgcggattg tttataatac agattccaag gatcctggga tcttctgccg gaacaaatcc
     4561 aagaaaagag gaaatgtatt tatttgcact tcgcggcagt gtctcggaag tcgctgtctt
     4621 tcctcctata tgatatcctt cgatataggc ctttttgccg cctccttcgg aaacgacttt
     4681 ttcgagaatc atcctcattg tcttggaaac ctgctccgat acgatctgtt ttccttcctt
     4741 ataagaaagt gtttccagca aagtaccgtc atcttcccgc acttccaccc caaaatgcgg
     4801 cgtgatctta tggccgccat tgatcaggga acacaccgtt gctgccagac ggatcggcgt
     4861 gatctgaaag gactgtccga acgagatcgt tgccagttcc accggcccga tatccttttt
     4921 ctgatgcatg atcgtccctg cctctcccgg aagatcgatc ccagtctttt cgagcagacc
     4981 gaactgctga aaatacctgt aaaaagcatc tgtccccagc cgctgtccca cttcaataaa
     5041 aaccggattg caggaattca tgaccccctg cacaaacgtc tcacttccat gaccggtcgt
     5101 tttatggcag cgtatcctcc tgtcatcgac aatccgaaat ccgggacagc tgaaagtatc
     5161 ttccggtgtt acaacgccct cttccagagc tgccgatgcc gttatgatct taaatgtcga
     5221 acccggctca tacgtgtcgt tgatgcagcc atttcgccac attttgtttc tttcatcctg
     5281 aatttcctgc tgtgacatgc cattcgtact ttttttcagg ttaaacggat cattcagatc
     5341 gtattccggt acatttacca tcgccaggat ctccccgttc ccgggccgca tcagaataat
     5401 ggagacgctg tctgcctgtt tctgttccag tgcctggtaa gccgcctgct gtgcatactc
     5461 ctgcaggttt gcatccaggc ttaagattag atttttcccg gaaaccggct cttttcttcc
     5521 ttctccggtg tcggataatt ccacacctct ggcatccgtc atggtcagaa tctgcccgtc
     5581 tgttcctttt aagatgctct catatttgac ttccagtccc acgatcccct gattgtcact
     5641 tccggtaaat ccaagcactt tggaagccag atttccatat ggataatttc tcttatagtc
     5701 ttcatccact ttcacgccag ccagatcgta ttctcttatt ttatccccgg tctgtttatc
     5761 cacattcgtt ttgatccgct caatggaact gttcttttcc acgcgtttct tgacctggtc
     5821 acgctccagc cccagttctt ctgtcagcac atcgatcact ttttccggct ctttgatctg
     5881 actgtggatc acagaaatcg tacataccgt cttattatcc gccagcactt ttccgttgca
     5941 gtccagaatt tttccgcggg ccgctttgat acttcgctcc ctctcatgga gatcctgtgc
     6001 tttttcgctg taataatctg cccgaagcag catcagatac cccagccgcc caatcagaaa
     6061 aagcatcgcc gccatgcaca gtgcaaaaac agccacgatc tttttgcggt gaaatgtttt
     6121 gatctttaca aacaatacaa aaaacctcac aaaagctttc tattattata tcgctgcttc
     6181 tgtgaggtta aaactgcaaa ttctttatat gatgccaggg tcaaaaggtc taaatccaca
     6241 tgagcttgct cataagtgga tgaaagacct tgggacccgc cccgatatga gcataaaagt
     6301 gggatgata
//
LOCUS       DAWDWH010000114         6304 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5437_length_6304_cov_11.218115, whole genome shotgun sequence.
ACCESSION   DAWDWH010000114 DAWDWH010000000
VERSION     DAWDWH010000114.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6304)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5437_length_6304_cov_11.218115"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4501..4510
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4977..4986
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1943 a         1285 c         1509 g         1547 t
ORIGIN      
        1 tgacgaatat ccgattgtgt tatatgataa aaaaatgcga aaaaataatt tctgcaagta
       61 accgtcaacc aaatatacag actatgcaca gaaataagga taaatgattc aatgagaaaa
      121 ttagctttaa gagatgaaat tttattaagt atagaaaaac ctgcccgtta tattggaaat
      181 gaagtaaatg ccgttatgaa gagcagggac gtggacatcc gctttgccat gtgcttcccg
      241 gatgtctatg agatcggtat gtcacatctc ggtattcaga ttctgtatga tatgttcaac
      301 aaacgggagg atacctggtg tgagcgtgtc tattccccat ggaccgatct ggatgccgtg
      361 atgcgcaagg aacacattcc gctgtttgcc ctggagtccc aggatccgat caaggatttt
      421 gactttcttg gaatcacgat ccagtatgag atgtgttata ccaacatttt acagattttg
      481 gatctttccg gtatcccgat gatggcagtt gaccgtacct gggacgatcc gatcgtgatc
      541 ggcggaggtc catgtgctta caatccagag cctctggcag atttctttga tattttctat
      601 atcggagaag gcgaagtcgt ttacgatgaa ctgctagaac ttttcaaaaa agcaaaaaag
      661 gaaggctgga gccgtgaaga gtttctccat caggcatccc atattgccgg tatttatgtt
      721 ccgtctcttt ataaagtatc ttatcacgag gacggtacca ttgcagcctt tgaaccggtt
      781 tatgatgatg taccgaaaac cgtcgaaaaa cagatcgtta tggatgtgac aaatgcttct
      841 tatccgaaaa caccggttgt tccgtttatc aaatgtacac aggaccgtgt cgtactggag
      901 attcagcgtg gctgcatccg cggctgccgt ttctgccagg caggtatgat ctaccgtccg
      961 accagagagc gtgaccttga aatgctcaaa gaatatgcct atgccatgtt aaagagtacc
     1021 ggacacgaag aaatttcctt aagctctttg agttccagtg actactcaga tcttgccggg
     1081 attgtaaatt tcctgatcga tgagttcgag aaaacaggtg taaacatttc ccttccgtcc
     1141 ttgcgaatcg atgcgttctc actggacgtt atgagcaaag ttcaggacat caaaaaaagc
     1201 agcctgacgt ttgcaccgga agccggttct cagagactgc gtaacgtcat caacaaagga
     1261 ctgacagaag aagtgattct ggatggtgcc ggaaaggcat ttgacggcgg ctggaataaa
     1321 gtaaaacttt attttatgct tggactgcca acggaaaccg aggacgatat gaaagatatc
     1381 gcacatctgg cagataagat tgcaagacgc tattacgaaa tcccgaaaga ccagagaaac
     1441 ggcaagtgcc agatcacggt cagcacttct ttctttgttc caaaaccatt taccccgttc
     1501 cagtgggttc cgatgaacaa ccgggacgaa tttctcggaa aagccaagat tgtaaatcag
     1561 gaagtcaaag accagctgaa tcgcaagagc atcaagtaca actggcatga agcagatgtc
     1621 accgtactcg aaggtattct ggcaagaggt gaccgccgcc tggctcctgc tatcctgcga
     1681 gcatacgaaa agggagcact ctacgatgcc tggggtgaaa atttcaaata cagccgctgg
     1741 agtgaagcat ttgatgaatt tcatcttgat ccggcttttt ataccgagcg tgagcgttcc
     1801 tacgaagaaa tccttccatg ggatttcatc aggatcggcg ttaccaagaa atttttatgg
     1861 aaagaatggg aacgtgccca gaaagaagaa gtaacgaaaa actgtcgtgc cggctgttcc
     1921 ggatgcggag caagtgtatt tggaggaggt gtatgttatg aaggtaagaa ttaaattccg
     1981 caaacagggt gtcatgaaat tcatcggtca tctggatgtg atgcgttatt ttcagaaagc
     2041 aatccgcagg gcagacattg acatcgccta cacagaagga ttcagcccgc atatgatctt
     2101 atcctttgca gcaccgctcg gtgtcggact gaccagcaac ggcgagtatg ccgacatcat
     2161 gctgaagaca ccgatctcat ctgccgaggc agtcagacgc ctgaacgatg tgatggtcga
     2221 aggcatggag atcgtaagtt tccgtgaaat tccggatgaa aaggcaagca atgccatgtc
     2281 tttagttgca gaggcggact atactgtaat atttcgtgaa gggatggagc cgcctgccgg
     2341 atgggaagaa aagcttctgg cattttatga gaaaccttcc attcttgtga caaaaaaaac
     2401 aaagaaaagc gaacgtgaaa tggatctgaa gccgctgatt tatgcacttt ctgtctctga
     2461 cggaaaaatt ttcatgacac tctccaccgg aagtaccgac aacatcaaac cggagcttgt
     2521 catggaagca ttctgccggg aaattggtta tgatctgcct gcttttgcac ttcttatcaa
     2581 ccgggaagag gtctacgcag aagggcatat ttcactggaa gatctgggaa aagacattgg
     2641 ctgatacact gatcatcaca aagagagatg cacagaccat ttggatggcg gcgttatccg
     2701 gcacgcatgc cacagatctg cgtattgctt ctgcatccga tgaaagcaca tcgcttgtcg
     2761 gaaacattta tatcggaaaa gtacgcaaag tccagaaaaa cattgatgca gcatttattg
     2821 aaattgcaga ccgacagcag tgctatttta atattgccgc aaacccggat gccatctata
     2881 caaaaaaagg caattcaaaa aaaatcgcag aaggagatga actgctcgtt caagtgatcc
     2941 gtgatggctt aaagacgaaa cttcccgccc tctccggaaa cctcgaactt ccgggacagt
     3001 atcttgtgct gaccggtaat gacagtcgca tcggtatctc cggcaaactt tcacagagca
     3061 caaaagaacg cctgaaagaa tggatggaac catttgcacg cgaaaaatat ggctttatcg
     3121 tgcgtacaaa cgccggcgaa gcttccagag aagagattga agcggaagcg aaaaaactgg
     3181 aagaacagta tctgcatctt ttaaaaacgg caccttaccg gacctgtttt tctgctttaa
     3241 aaacgtcaga accggtctgg atcgagatgc tccgcaatac ttacactgca gatctgacct
     3301 caatcatcac agacgatcct cagatttata actctctgga acattatctg aaagaaaacg
     3361 atctgcagaa tctttccaaa ctccgtctgt atcaggatga tctgcttccg ctctacaagc
     3421 tctgccgcct ggaacgagag atcgatctgg cgacctccag acaggtttgg ctgaaaagcg
     3481 gtggttatct ggtgatcgat ccgacggaag cactgaccgt cattgatgtc aataccggta
     3541 aatattccgg taaaaaactg cggcgtgaaa cattaaaaac aatcaatctg gaagcggcga
     3601 aagaagcagc aagacagatc cgcctgcgga acctttccgg tatcattctg atcgatttca
     3661 tcaatatgga aaacaaagaa gatcagaagg aactgcttgc atttctggaa agccggctgt
     3721 ttgccgatcc tgtcaaaaca agactgatcg atatgacaaa gctggagctt gtggagatca
     3781 cccggaaaaa agtccataaa actttaaaag agcagattgc agcactggaa agcgaaaagg
     3841 aatctctttc acagaaagat taaaaataat gaaatatttt gcaaaaaaca gttgacgtaa
     3901 attctaagac atgctatcat gtagcagatg ccgcataaag aggtttaaat gtcatgccgc
     3961 aaggcagact accatattta gcgagtaatg ataataggag gtgccatatg tacgcaatta
     4021 ttgcaacagg tggtaaacag tacaaagtag ccgaaggcga tatcattaga gtagaaaagc
     4081 ttggtgtaga agctggtgaa acagtaactt tcgaaaatgt tcttgcagtt agcaatgacg
     4141 gactgaaagt tggtgcagat gtagctggtg caacagtaac tgctagcgtt gttaagaatg
     4201 caaaagcaag aaaagttatt gtttacaagt acaagagaaa gaccggatat cataaaaaga
     4261 acggtcacag acagcagtac actcaggtta agattgaaaa aattaacggt taatccatat
     4321 gattacagtt acaatccata gatcaaatga cagttatgct ggttttaaag tagtcggaca
     4381 tgccggttat gctgaagaag gttatgatat tatctgtgcg gcggtatccg tacttgttgt
     4441 aaatgctgta aattcgattg agacttttac agacgataag tttgctgccc gtgaagatca
     4501 nnnnnnnnnn aatcgaggag gtgtagacca tgttgaagat gaaccttcag gtattcgctc
     4561 ataaaaaagg tgttggttct acaaagaacg gacgtgactc cgagtctaaa agactgggtg
     4621 caaaaagagc tgacggacag tttgtaaagg ctggtaacat tctttacaga cagcgtggaa
     4681 caaagattca cccgggcgtt aatgttggac gcggcggcga cgataccctg tttgctttag
     4741 tggacggtgt tgtacgtttt gagagaaaag gccgtgacaa aaagcaggtt tcaattgttc
     4801 ctgtagaggc aaaataataa gtggaacatg tggcttcaaa tcgtttacgg tttgaagcca
     4861 tttttgatat aaaggagtat atatgtttgc agacagagca aaaatattta tccgttccgg
     4921 taaaggcgga gacggacacg taagtttccg aagagaactt ttcgtgccga acggcgnnnn
     4981 nnnnnnttgg acatgaattg ttacgccacc tcgaacgtac aaaggtcatc ctccatgtcg
     5041 tggatgcagc ttctacagaa ggccgtgatc cggtggatga tatttacaag atcaatgaag
     5101 aactcaaagc ctataatccg agaatggcaa ctctgccgca ggtgatcgct gccaataaaa
     5161 ccgatgtgat ctattctgag gacgaggatc cggttgaacg catccgtgca gaatttgagc
     5221 ctcagggcat gaaagtattt tccatttccg cagtaagcgg caagggatta aaggaacttt
     5281 tatactatgt gcgtgaactg ctggatgcac tgccaaaaga agcggttgta tttgagcagg
     5341 aatacgatcc gaacgagtat aatccaaatg acaaccttcc ttataccgtt gagaagtcag
     5401 aagaagagga aaacacttac gtggtagaag gtccaaagat tgaacgtatg cttggttata
     5461 cgaatctgga ttccgaaaaa ggcttccagt tcttccagaa cttcctcaaa acaaccggca
     5521 ttctcgacga actggaagct gccggaattc aggagggcga taccgtccgt atgtacggcc
     5581 tgaaatttga ttactataaa taaagatttg atatgataag cgaggaatat aattatgaca
     5641 agtaagcagc gtgcttattt aaaaagtctg gctatgacca tggatccgat cttccagatc
     5701 ggcaagggaa gcctgacacc agaaaacacc agtgcgattg ctgaagcact ggcagtacgt
     5761 gaactgatca agatcaatgt actgcaaaac tgcatggacg atccgaagga acttgctaga
     5821 accctggcag aacgcaccca ttcacaggtt gtacaggtta tcggcaaaaa aatcgttctt
     5881 tataaagaag gaaaagacga caagaaaaaa atcgaacttc cacgttgagg ggaggcattt
     5941 tcttgaagaa agaaaaagca agaaaaattg ggatcatggg aggaaccttt gatcccatcc
     6001 acatcgggca tctgatcctc ggcgaaaatg cctggcatca gtttgggctg gagaaagtac
     6061 tttttatgcc ttctgccaat ccgcctcaca agccaaaccg tatcggccgt gcggatattc
     6121 aggaacgtgt tgatatggtt cgcgatgcca ttgcagacaa tccgcatttt gaactttctc
     6181 tggaagaagc ctttcgggaa gattactgtt atacaagcga aactttgcag gaaatgactg
     6241 taaagcatcc ggatacggaa tactatttta ttatgggagc cgattctctg tttgcatttg
     6301 aaac
//
LOCUS       DAWDWH010000115         6265 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5476_length_6265_cov_14.022061, whole genome shotgun sequence.
ACCESSION   DAWDWH010000115 DAWDWH010000000
VERSION     DAWDWH010000115.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6265)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6265
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5476_length_6265_cov_14.022061"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    246..255
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5529..5538
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2047 a         1343 c         1563 g         1292 t
ORIGIN      
        1 gggtgaaaaa ggatataata gcagacagta tggtgcctga aagaggattt tttgcggccg
       61 gatgccggag gtattcttga atgaatggca ccagttgatc aggaggaaaa aacagtgaaa
      121 aaaacaatga aaaacagact ggcagtcgca ggactgtgct ttacgctgac agcagggatg
      181 ctgaccggat gcagtggaaa ttcaggcaag gcgatcatta cattggacgg acagaaaaca
      241 gaatannnnn nnnnncagtg gcaaatatca tgcttcgtta tagccaggca cagatgcagg
      301 cattttacgg agcttatctt ggagataatc tgtggtcaca gtacggcgat tccacaaaga
      361 gcacgatgat ggataccctc aaacagatgc tgatcctcga acagcatcag gacgagtata
      421 atgtgagcct gacagacgat gacaaaaaga agatcgatga agctgcacag cagtttatgg
      481 atgacaacga ccaggcaacc ctgaaatcca tgggtgcgac aaaagaacgt gtggcacgtg
      541 ttctggaact gtacacgatc cgcaataaaa tgtcaaatgc gatcgttgca gatgttgata
      601 ccaacgtaac tgatgacgaa gcagcccaga aaacgatcaa ttacgtggta ttcagcactg
      661 cagatacgac cgattcggat ggcaataaag tggtactgac ggatgatgag aaagcagctt
      721 tgcttgcaaa tgcacagaaa gtacgtgatg cagcagcagg cggcaagagc atggaagatg
      781 cagtcaaaga tgtcgatgac accaagaccg tgagcagcac ctcttatggt gataatgatg
      841 aaagttacac cctggatgaa gcgatccgca atgcggcaga taaactgaaa gacggagaag
      901 tggcagatga agtgatcacc acagatgcag gatattatgt catccagatg aaagcaacct
      961 atgatccgga tgcaactgca agcaaaaaag aacggatcat caacgaaaga aagagcagca
     1021 aattcagcga agtctacgat gcatggcaaa gtgcagcaga ctacacacag gatgatgctc
     1081 tgttaaaaga gatcaccttc catgatattt accagatggc aaccgaggct cagagcgaga
     1141 ccggaacgga aagtacagaa agtaccgaag atgccggaag cacagaggca accgaggctg
     1201 caagtgagac agagagtgca gctgcaacag aataattatc atgctggacg taaaaaggca
     1261 gagcacgtga cacgcatcgt ttcctgttcg gtttgtgccg tctatgatcc gccgttcgct
     1321 ggaactcgca gcttcgctgc tcagacaccg ctcggcggcg gaaactagac acaaacctca
     1381 cgacggaacc ttgctaccgt cactgtgcag ctgccttttt tctgtccagc atatacattt
     1441 ctcaggcttt caaaattacc gcaagatggt acggagtttc ttttgattca gaaactggtg
     1501 ataaaatcgc tagggtatag atacgggtag attttatgag tgtgcagaag aacatataat
     1561 gtatgatata catcttttgg caaaataata aacgtattct gaaatcagga agattggaac
     1621 tgccagatgc attagtggga aaaacagcac agtcataaca atcagaaagt aagtcaagct
     1681 gacggtagcg aggcttccgt cgtgaggttt gcgatgttgc cgccccggag agccgctgtt
     1741 tgagcctcga agaggcgagt ttaggcgaag ggcggctaac acacgagcga accgaacagg
     1801 aaacgatgcg tgtcagcgga tttactttct gatgttatga cgtcctcttt aactatagtg
     1861 aagttcaaac taaaaacctt gacgttccac aggttttatt gtatgataat actgaatatt
     1921 ttcggtgacg cgaatcagga tttcgtatta ctgaacaact tggggagatt tcggagggga
     1981 atatccccac ttttagtcag ttaaggagga aaagtatgag aagaaaaagc agcaaacagt
     2041 tgttttctgt actgatggca accgctatgg ttatgtccgg catcagcgtt ccggttatgg
     2101 catcaccggt tgacgacgct atggtacagt ctgctctggc agaagccggc gtagagacat
     2161 ctgaaagcag agagatcaat ttcaacaaag gctggaagtt ccactttgga gatgtgaccg
     2221 gtgcagaaac aaaggattac gatgattcca aggcagatga atggggagac ttaaatcttc
     2281 cgcacgactt cagtatcaca caggagccgt ccaactccaa cgaagcagag tccggattca
     2341 tgccgggtgg tacaggatgg taccgcaaga gctttaccct gccgagtgat tacgcaggca
     2401 agagtgttgt cctgaacttt gacggatctt ataatcatac atatgtatat gtaaatggca
     2461 caaaagtagg cgaaaaccat tatggatata atgatttcgc atttgatatt tcaaaatacc
     2521 tgacctgcga cggcaccaca gagaacgtga tcagcgtaaa agtggtacat cagactccgt
     2581 ccagccgctg gtattcagga tccggtatct accgtgacgt agagctgatc gttaccgatg
     2641 ccgttcatgt ttccagaaac ggcacatacg tgactacacc aaatctggcg acagaaaaag
     2701 acggcgatgt taccgttaaa gtacagactg atgtccagaa tgacagcagt gcacaggcag
     2761 cagctcagat ccgcacaaca gtcctggatg ctgagggcaa agcagtttcc aaggcagtga
     2821 ccaccgatgt gacactggca gcaaacagca cagcagaaaa agaacagacc ctgaaagtaa
     2881 acaaaccggc actgtggagc acagaaaatc cgaacctgta ctatgtacag accgaagttc
     2941 tggtagacgg caaagtaaag gacacttata agacaaccta cggtttccgt tacatcaatt
     3001 ttgatgcaaa taccggtttc agcctgaatg gcaaaaatgt aaaactcaaa ggtgtatgta
     3061 tgcaccatga ccagggtgca ctcggagctg catcagaacg tgatgcagta taccgtcagg
     3121 ttaaaaagct caaagaaatg ggatgtaatg cgatccgtac ttctcacaac acaccttctt
     3181 ctgtactgct tgaagcatgc aacgagctgg gtatgatggt tatggatgag acgtttgacg
     3241 gatgggcatt cccgaaaaac ggcaacagcc aggacttcag cacacacttc aaccagacca
     3301 tcagtgcaga caacaagctg cttggtgcaa cgacaggcga cacctggtac aaatttattc
     3361 tggaatcaaa tatcgagcgt gacaagaacg atccgtccgt tatcatctgg gatatcggaa
     3421 atgagctgaa cttcggtgtt accgatccaa gccagtacga acagtacgca aagaacatga
     3481 agagctatat cgaagcgatc gataagacca gaccgatcac agtcggcgat aataatccgg
     3541 gtggattggc aaatgccaat acaacagagt tccgtaataa ggtttctaca gttctggcaa
     3601 agaacggaga aggtcttgca ggtgccaact attctatggg agctatgagt ggtatccata
     3661 atactcatcc ggactggaag atcattgcta cggagactgc ttccccgagc aacagccgtg
     3721 gtatctacaa tacactgtcc cagtacaaca agacaggtga ctatcagtgt accgcatacg
     3781 ataccaacgc cgtatcctgg ggcaacacag cacgtgagtc ctggtggtac acgatcaaag
     3841 atgattttgt atccggtgag tttatctgga ccggatttga ctacatcggc gagccgacac
     3901 cgtggaacgg aacggataaa ggaagtgtca gcggtgataa actggcagta ccgaactcct
     3961 cttactttgg tgtgatcgac accgcaggct ttgagaaaga ttccttctat ttctacacca
     4021 gccagtggag agaagataaa aagacactgc acgtcgtacc gcagagctgg aatgcagaag
     4081 acttaagcat ctccggcggc aaagtaccgg tatacgttta ttcaaacgca gcaaaagtag
     4141 aactctactt gaacgacaag ctgatcggta cttccacaag aaccccgatc acaacagcag
     4201 ccggtcatga atgggctact tacagcaatg tatcaaacga tgcagatcag tgtacagcag
     4261 tcaacgaatc acatcagtgg aaagctcagg ctatccagtt caacgtaaaa tatgcagaag
     4321 gtacgctcag tgcaaaagcc tatgatgaaa gtgggaacga gatcaaagac acccttggtt
     4381 ctgcttccgt tacgacaaac agcgacaaag gcagcagcct tgcagttact gcagagaaga
     4441 cagaaattca ggctgacgga agcagtttaa gctacatcga tattgatgta aatgacaaag
     4501 acggacgttt tgtaagctcc gcagacaaca gcatccgctt tactttgaca ggtaacggaa
     4561 ccatcgttgg tgtggataac ggaaatccat ctaccgtaga taaattccag cagaaatccg
     4621 ttctgacaag cgacaagacc gcaaaaatca aggcattcag cggaaaagca ctcgttatcg
     4681 tacgcagcac agaaggtgca ggcggtttcg tactgaaagc agaatccgca ggcctgaaag
     4741 gggattctgt atttgtaaat acggtaggtg acaaaaaagg tgaagtattc ctgaaagatt
     4801 acaaggtcaa atcagaatac accgttacca tgggaaccaa accacagctt cagacagccg
     4861 taaccggtat catgagtgat gattccacac aggaaggtac gatcacctgg aatctgacag
     4921 atgagatgta caacactcct ggcgaaaaag aactgaaagg aacactgaaa gttggaaacg
     4981 aagaagtagc agtatctgca aaccttcatg taaaaccgat catcgtagca gtacagaact
     5041 acacaagagc aaccgtaaaa aatctggttc cgaccctgcc ttcaactgta gccggtatcc
     5101 ttccggatgg cacaagctat ggtgcatatg cagttacctg ggagtctatc aaagcaacgg
     5161 atctggcaaa cgtcggcgat gttgtaaaag taaacggtaa agttgacgta gacggcacag
     5221 agatgaccgc aacggcaacc gtacgtgtcg cagaaggcga agtaaaagaa gcagtcaacg
     5281 tagcaaagga atataagact ctgacagaga gctgcggaca gcctgcagat aatctgctct
     5341 ccatcgtaga tggtgtaaac aatgttctgg ataagccgaa tatgcgatgg acaaactgga
     5401 acgaccatct gctgaactct tcaccggtta ttacgtttac atgggatgaa gtttacgaac
     5461 tggcaagcat caatgcatgg ttcttcggtg atacgttcgt atccgcaccg gaaagcgtaa
     5521 ccatcgcann nnnnnnnngt ccgaagatgg tgaaaacttc aaagaagttg catttaccaa
     5581 gggagattac caggtaaacc agaagaacga actggtattt gaagatgtcc agaaagcaaa
     5641 agcactgaaa tttaccatga aacagcaggg aacaggctat gttggtctga ctgagctgga
     5701 gatctggaca agcacaaacg gttatacatc caacagcaca gcggaactga gtgatctgaa
     5761 agtaaacgga acagcagtag ccggctttgc agcaggcaaa ctggatggtt ataccgtaaa
     5821 cgtagacaga gcagacaaag cggttgtaga agcaactgca agagacaatg catctgtaac
     5881 agtagtacca tttgataaag atgacgttgt aaaggtaatc gttacctccg aagatggtaa
     5941 gaagaccaat acttacaaga tcaaactgaa tgcagagaaa aaagtagcag acaaagaaac
     6001 gatcgaaaaa atccagggtg agatcaagct ggcagaaaca attgagaaag cagagtacac
     6061 agcaagctct tatgcagcat atgaagcagc tctgaaagaa gctcaggata tcgtaaagaa
     6121 agacggagct acaagagaag aagtaaatgc agcacttgca aaactgcaga cagcaagagc
     6181 aaatcttgta aaaggcgttg atccgaagcc ggaaccgaac ccggaaccaa agccggaacc
     6241 aaagccggaa ccgacaccaa acccg
//
LOCUS       DAWDWH010000116         6264 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5478_length_6264_cov_12.031728, whole genome shotgun sequence.
ACCESSION   DAWDWH010000116 DAWDWH010000000
VERSION     DAWDWH010000116.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6264)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5478_length_6264_cov_12.031728"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1210 a         1609 c         1312 g         2133 t
ORIGIN      
        1 ttcctttgaa cagtgctttg tatgcctttt tctttgcttt cggtacgcga accactgcat
       61 gtttgttgat gcctttcagt gcctgcttac cgaccatttt cagctttttg cccttcacag
      121 tgatgctctt aagtctgctg tcaccataga atgctttctt gccgatcttc gttacgtttg
      181 caccgatcac ggctttggtg gctttcttct ggccggcaaa tgcctgatct gcaattgcag
      241 tcactttgca tgtcacatct ttgatggtta ccgttgctgt cacgttgatg gtcttgatgt
      301 tcttctttgc agtgccatat gcttctgctg tcttggctgc cgcatcggtt acacggtact
      361 gtacgccacc tgcggtaacg gtatcaccgt ttttcagtgt cagagaatct ttcagttttt
      421 caatctcttc tcttgctttc cgggcttctt cctgagcctt tttgattgct gcctcagctt
      481 ctgcactgtc ttttgctgcc tgatcctgaa ctttcttcag ttctgcctgc agagctgctt
      541 tttcttcttc cagagaagct ttttcgccgc tcagtgtcag gacttctgcc cttgctgtat
      601 tcaatttccc ctgaagtgct tcgatctgtt tttcaagagc tgttttttct tcggctgacg
      661 taccttccag tgcagccagc tgatcctgtg catctttgat tttatcttga agtgttgtca
      721 cattttctgt tgctgctgcc agttctctct ctttatcttc cagctgcttt gtcttttctt
      781 ccagctgttt tttcagatct tcatttgccg ccttgtctgc actttccagt cctttgattg
      841 catccgagat tgcactgatc tgtgcactta tttcttccac ggttgcattt tctgcatcgc
      901 agacttcttt tgctgctgcc agtgctgcgg tcagttttgc atagctttct gccgtataat
      961 tttctgcatt cagtttttct gccagatcaa tcactgcttt taatccatct ttgctgtgat
     1021 cctcttccgg tgtttcactc tgtttaaagt ttacggtgta ttcctgtgct ttgttttcat
     1081 cgccgttctc cggccggaag agcacttttg cagttccgtt tacggcatcc agaaccgtta
     1141 cggatgcatt gtttgttgcc tgtgctgtga tcacacctga tctggcatcc agatttaggg
     1201 tctttgtatc tgcatcaaac tgtgccagta cttcctgtcc gtccacttta atagaagtta
     1261 cttcgtaatc tccatatttc tcgatcgggg tatagtatac ttcatattct tccagtccgg
     1321 tccagttgtt gtcctgcggc gtcagggtga tacggatcgc tgtggtttca actgcttcaa
     1381 attctacatt gaccatctct ttgaaatttg caagagacgg tacggatggt ttgccgccga
     1441 catatgctac ttctgtccag ccggtgtgtg ctttcagagg gttgtctgct ccccagcctt
     1501 tgatattgct gcagctgttt gcagaatcgt tgcccagtct gtttgccggg atcacaccgt
     1561 cgttcgcact gtaatactcg atcttgtaat cttttggaag tctgactttg tttgctgcac
     1621 ttccatcttc ttccataaat ccaatggaaa ctttatttac ggtatatttc ttctgatctg
     1681 aatctgcggt ggaatcttct tttccgaagg cactgatcac aaacggcaga tgatcggagt
     1741 ctcctacggc tgcatctgcg ttggtatgat atttgccgct ttcccagtca caccagattt
     1801 tcttgccgga tgtgctgtca aaatttttat ttccatcaac tgcattatat acaagatcgt
     1861 tggaagatga aaaatatgcg aaagtccttg gccattctct tgttttagag gaaccgtctg
     1921 cattcagtgc caggtttgta tcggtcggtg tattgtttgc atctgctgtc acatctgcca
     1981 cacggaccga cgcttttgcc tgaagtacag tttccctagt aactgctttt ccaactgttc
     2041 cgtaaacttc tacgattcca ggattcctga aatctgcatc tgcaagatcc catacaactt
     2101 cctgctgctg tttttcaccg ttgctgtagt atacagttac tgtttccggc atggaaggtg
     2161 ttgtcccttt tgctgttgcc acggatacct gctgcacacc tattacggca gttacagtta
     2221 tttctgctgt gatgcgtgca ttggtgtcaa ccacggttcc gcccgtgcta aaggttccga
     2281 tctgttttaa ctgttctgca ctgatttcat cccattgtac atccagtacc tgatcaccga
     2341 cgttggtatg tgcaatgacg gttcccggaa gagccgggca gactcctttt gctgttgtaa
     2401 ctgcgatcgc ttcataatcc gctacagtta tatctactgc attaatcacg aagttttctt
     2461 ctacaacacc ctggtaagca ccttttcctg tcacaacaat cgttgctgtt ccaggttctg
     2521 tgttattttt ataacttact tcatagtctg taccttctgt cagggtcact gcctgtgcat
     2581 cttcggatgc tttgtaagta acaaccggtt tcggtgtgag tgcacttccg gtgtaatcaa
     2641 agctgcgatc ttccagtgtt acggttgtat tgtcacggta aatttcctgt gcagcgtagc
     2701 gtacccttac ttctgctgca gacatatatt tatttacttc tgctgcagac tgtcctacgg
     2761 ttgacacacc atagagacgg atatattttg catcgaccgg tgcaaactgt gccagtttcc
     2821 attctacgga tttactccat gtgctgtctg catctgattt tgcaacttcc gtccagtttt
     2881 tgccatctgt acttactttg atgctgtact ctgtaacgat accgtttgtg tcgctgcttc
     2941 tcggcagata acgcagtgca ttgatctttt ctgtttcttt cagttcaatc tgcaagtaac
     3001 ggttatccgg atcatttcga aggtctgtcg gtccacttcc gcttccccag tttgtatgcc
     3061 agatggtacc ggtctttcca tctgccgctt ttgcaacaga accgctgcct tcttcacttg
     3121 gtgcacgcag tgtcatgttc ctgtaatcat aatccataga tgcatcggaa gcactgtcgc
     3181 ttgctgtatt gtcttttccg agcataaccg gatagatggc acgcatcaga tgatcttcag
     3241 attcaataag gatacggatc acaccgtctg catctttgtc aagaatggtc acgcttgcat
     3301 tgtcttttcc ttctgctgta aggtctgccg catcaaattc tgtttcattt acgccaaacc
     3361 agcctttttt caggcttgct tcatccgcaa tgtgaccacc cactttcaga ctggaaagtg
     3421 ctgcttctga tcctaccgta aagctcggga tccttgtgtt catttccagt tccgtcaggg
     3481 tggcatcttt ggtaaattct acacggaaat aggtttcgga aacggtttct tttacattgt
     3541 aagtacgaac cgtcacacct ttttcttttt tatttgttac cgtgcagtct accggcgtcc
     3601 agttgactcc gttgtcgccg gatgcataga ttttgatcgt tccggaagtc ggtgcggtat
     3661 ctttcagata cattgtgaat cctttcaatt caatcgctgt atccagacgg aaatcgaggg
     3721 ttccttttcc gctccatgcc acatcatcgg cacttgtctt atcatcctgc agtttcgcca
     3781 ggacatctgc aacagatgca tcttctttgc tggatgtacc gttgtaatac atttccggca
     3841 cgttgccaag ggcttctgca ccgtctttgt attctccatt ggtaactctg actgctgcag
     3901 tcacttcaaa gttctggtca aatacggttg ccgttccttt tacattcttt gttccgattg
     3961 tcgctgtcag atcttttgga atatcccact ttaccggaaa ttctgctgcc aggattgttc
     4021 cgtctgcaag aactgccggt ctggctgcag gaaggctgac ttctgcatct tttccgactg
     4081 ctgcggaata attcaggaca cctgctgtct ggccgattgc actgacattg accgtgatac
     4141 gcatattcag atctgctacc gtaccggata ccgaatacgt ttcttcttct ccggtaaatg
     4201 catcccaggt tactttcttt gtctcggaag tatcgtcaga ataatggatc tttacttcgg
     4261 atgggagaac tggtttcgtt ccctgtttta cataataatt ctttgcgatc tcatagctta
     4321 caacggtctt ttctccggtt actccactgc cgtctgcaac tgtggtaacg acagtctgtg
     4381 ctgcagtgta tccgtctgaa gttgccttta cggtaaagct tccggcatct tttgtggact
     4441 gaacgatcgc gatcagtttt cctttaccgg cttttctggt cggttcactg taagaagtgg
     4501 tatcgttctg aacaccgtta tcaagtgaaa ggagcttgcc gtcaccttct acggcaacat
     4561 ggatctgcgg ctccgcaccg tttacttctc tgccgtctct gtccgttaca tcgatctcaa
     4621 caaagcttaa atctttgccg tctgccgtga tctcgctgcg gtctgctttt gcggtaagtt
     4681 tggatccctt tccggtcgtc tctgccacat tgcgtccgtc agtgtctttg atctcagtca
     4741 caccgtctgc ttcaaatgct tttgcttcca gtgttcctgc ctcataaggt acctgaaatg
     4801 ttgcatacag ggaagtatgt ccgctcgcct tgctgctgtc aaagcatcct gttccgcttg
     4861 tataattctg ataaccaccg gtcggtgtat cggtgtgtgt cgctgtcgca cttccgattt
     4921 ctttgccgtt cagataaagt ttcacaacag gagcatcact gtaaactaca atctctgcct
     4981 tgccgctgtt atcgagcatg atctcatctt cattccatac cgggagaaca tgaagggtat
     5041 tttcgttgtc attccactgg ctctgataca gataatagga atcttttggg atacctgccg
     5101 tgtcaatgat accaaagtaa gagttctttg caatgttcgg ccaggttccg tttgcaccgg
     5161 ttccctgcca gttatatggg gtcggttcac cgatataatc aaaacctgtc cagacaaatt
     5221 cgcctgcatt aaattcttgc tgcatcgtga tccacagccc tgcacttgcc agatgtcccc
     5281 agcctactgc acttttatca taagaagtca gtcggcggtc tccgcttcca ttgtcactgt
     5341 tgctgttctt cctgtcgtaa acgccacggc tgtttacgga agaggctgtc tcagaaccat
     5401 agaccagcca tcctctgctg tgtccgttgc tgatctgtcc ggagcttccg tagttaaatc
     5461 ctaccagacc tcccggcacg ccatattttg ctgcttctgc aaaaacaagt gctgtgttta
     5521 cctgattatt gtctgcccag acattgctct ttacctgatt gtcaccaaag gttacatatc
     5581 tggtcgcatc ttcttctccg gtccaggtga tcagtttttt tgccacttcc ggatactgac
     5641 cggttacatt cccgtcaatc agctgctgaa agatttcatt tccaaggctc cacatgataa
     5701 tggaaggatt gtttcttcct cgtcttacca tggctttgag gtcaaattct gcccattttt
     5761 cgccctctct gccatttacg atctggttgc cgctctcaat ggtctggtag aaccatttac
     5821 cataatcctc ggtatttcct gcttttccgg aaagccagca gtcaaaggct tcttccacca
     5881 gcatgatgcc ttcacggttg cacacatcga tcagtacctg ggatgccggg ttatgtgtta
     5941 cacggatggc attgactccc atctgtttta actcctgtac ctgacgttcc acagaacgct
     6001 cccaggcttc ggatccaaga gaaccctggt catgatgcat ggacactccg tgcagtttga
     6061 tgttctttcc atttaaatag aaacctttct ctgtagtaaa gtttacccag cggaagccaa
     6121 agtcagaatg gtagctgtct tttaaggtat ctccaacata aacttctgtt tttacggtat
     6181 agaggtttgg attttccgta tcccagagct ttgggtctgc aacggtgaca ctggcctcga
     6241 tcctggctgt tttgcccgct gcaa
//
LOCUS       DAWDWH010000117         6263 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5480_length_6263_cov_9.980670, whole genome shotgun sequence.
ACCESSION   DAWDWH010000117 DAWDWH010000000
VERSION     DAWDWH010000117.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6263)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5480_length_6263_cov_9.980670"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    971..980
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1436 a         1636 c         1228 g         1953 t
ORIGIN      
        1 caacacctgt accacagtca tcacccatgt taccaaatgc catcatctgt acgtttacag
       61 cggttcccca tgaatatggg atatcgttgt cacggcgata tacatttgct cttgggttat
      121 cccatgaacg gaatacagct ttgatagctt ccatcagctg ttccttcgga tctgtcggga
      181 agtctttacc gatcttctct ttgtattcag ctttgaactg ttcagccagg tttttaagat
      241 catccgcatt caggtctacg tcctgtttca cgcctttttc ttctttcatc ttgtcgatca
      301 gctcttcgaa gtatttttta cctacttcca taactacgtc tgagaacatc tggataaatc
      361 ttctgtagca atcccaagcc caacgtggat tgccggatgc ctgtgccagt gtctcaacaa
      421 cttcttcatt cagacctaag ttcaggatcg tgtccatcat accaggcatg gaagctcttg
      481 ctccggaacg aacggaaacc agaagtggat tctctttatc tccgaacttt ttgcctgtaa
      541 tgccttccat tttatcgatc gcttccatga tctgtcccat gatttcgtcg ttgatctttc
      601 tgccatcttc atagtactgt gtacaagctt ctgttgtgat tgtgaagccc tgtggtaccg
      661 gcagaccaag gttggtcatt tcagcaaggt tggcaccttt tccacccagc aggtttctca
      721 tggtggcgtc gccttccttg aacatataaa cccattttgt tgccattgtg ttatttcctc
      781 ctcaaaagta attgtataca gttcagtcag aactttttaa ctttcataca gtaatattct
      841 aacataaatc tgttctcagt caagctattg tccacaaaaa aagaagtttt tttgttattt
      901 tttcagaaca agatgctgtg gaagaccatt taccataaat ggcggataat ctccctggat
      961 ctacggttca nnnnnnnnnn acggttcaat gtaattgata aattcttctg tcacataatc
     1021 tgctgtttcg ttgagccatg ctctcggaac cgttttttcg ttgtttgcga tcttgtgaac
     1081 atcataaatg ccagccgcac tcatgtacgg atcgtcggat acgcggtcaa taacgaccat
     1141 cttgccgctg tctccttcat ctgctgcttt gacggctgca ccgcctacca taaacgcttc
     1201 gttgatatcc acacgggatg ccatatggga agcacttcgc tgaagggtgg aaaattcgac
     1261 tgcccttgtc ttgcatccga tctctgctcc caggaaattc gccagatatg cggccgttcc
     1321 ggtcagctgc ttgtgtccga atgcatccac ataatctccg ccgccggaaa gttcacacac
     1381 ataacgtccg tccggaagtt tgattccctc agacaccgct acgatgatgg attttttctc
     1441 tttgagcaga tctttgatct ttaaaaggaa tacatcgata tcaaacggca cttccggcag
     1501 ataaataaga tccgggcctt cgcattcttc ggtctttgca agtgccgtcg cccctgtcag
     1561 ccatccggca ttccttccca taacttctat gatcgtgatc atctcttttt tgtaggtgag
     1621 ccccagtgca tcgcggatca cttcttttgt cgatgctccg atgtatttgg ctgcacttcc
     1681 aaatcccggc gtgtggtcag tcagtgcaag atcgttgtcg atcgtctttg gcacacctaa
     1741 aaatttctgt ttatgacctt tgacaatcgc ataatccgaa agttttttga tcgtatccat
     1801 ggaatcattg ccgccgatgt agataaatgc ctcgatctcc agcttgtcca ggataccgaa
     1861 aattttctca taaatctcca tgtcctcatg gatttccgga agtttgtaac ggcaggaacc
     1921 aagaaatgcc gaaggtgtcc tcttcagcag ctcgatatcc atatccgaat gaatctgcgt
     1981 ggaaagatcc acgtactgct catccagaaa tccctggatg ccgtgaagca taccgtatac
     2041 tttgtcaaaa ccacgttcaa tcgctgtctt gtaaaccccc gcaaggctgg aattgataac
     2101 cgaagtcggc ccgcctgact gtcctacaat aatgtttcgc attcccataa cacaaatcct
     2161 ccttaatact ttattccggt gcaggcatat catatttccg tcttctgacc ccggccgatg
     2221 cctgcggatt gctccgttgc aaaaatataa cgatgcataa tgatgcctta gaattgctcc
     2281 gttacaaagt aactcccgca attctataaa cattataaca taaaagcaga aagactcaca
     2341 aggttctttc tgctttttgt atagtttatt ctattttact tatgcatttt tgtgtatttc
     2401 gtacagtttt caggcaattc ttgtcagaat tgctgctcgc ttcttagtat gccagctttt
     2461 cttcgaaata ggttgccaga tcttcgatct tgatacgctc ctgctccatg gtgtcacggt
     2521 cacgtacggt aactgctccg tcttcttcag aatcaaagtc gtaggttaca cagaacggtg
     2581 taccgatctc atcctgtctg cggtaacgtt ttccgatgtt tccgcggtca tcgtattcac
     2641 agttccattt ctttgagagc tgttcgaata cttttgtcgc accttcgttc agtttcttgg
     2701 acagcggcag aactccgatc ttaaccggtg ccagtgccgg atggaaatgc agaacggtac
     2761 gcatatcgcc gccttccagt tcttcttcgt cataagcact gcacagaaat gccagaacca
     2821 cacggtctgc accaagagaa ggctcgataa catacggaat gtatttttct ttcttctcat
     2881 catcaaaata gctcatatcc tggccggata cattctgatg ctgtgtcagg tcatagtcgg
     2941 tacggtctgc gataccccac agctcgcccc atccaaacgg gaagaggaac tcgatgtcgg
     3001 ttgtaccttt gctgtagaag cacagttctt ccggtgaatg gtcacgtgct ctcatctcgt
     3061 cttctttcat gcccagattc ttcagccagt cgatgcagaa tgctctccag tactggaacc
     3121 agtcaaggtc ggtacccggc tcacagaaga actccagctc catctgctca aactctctgg
     3181 tacggaatgt gaagttaccc ggtgtgatct cgttacggaa tgatttaccg atctgtccga
     3241 taccaaacgg gattttcttt ctggatgttc tctgtacgtt tttaaagttt acaaagatac
     3301 cctgtgctgt ttccggtctt aagtataccg tattctttgc atcttctgtt acaccctgga
     3361 atgtcttgaa catcaggttg aactggcgga tatcggtaaa attatgtttt ccgcaggtcg
     3421 gacatggaat ctgatgctct tcgatgaaat cacgcatctg ttcctgtgtc catccatcga
     3481 cagagccttc cagtgcaatg tctttctcat cgcagaaatc ttcgatcagc ttatctgcac
     3541 ggaaacgctc atgacattct ttacagtcca tcagcggatc agaaaagcct ccgagatgtc
     3601 cggatgctac ccaggtctgc ggattcatca gaatcgcaca gtcaacacct acgttgtatg
     3661 gattctctgt gatgaatttc ttccaccatg cctgttttac attgttcttc agctctacac
     3721 caagattacc gtaatcccag gtatttgcca gacctccgta aatctcggaa cccggatata
     3781 caaaacctct ggattttgcc agtgccacga tcttgtccat tgttttttcc atttgtctcc
     3841 tcctgtcggc cgtctctgcg gcatatgcat atattgaaag aagccgttta tgtttacttc
     3901 tttgctcgct gtttatcgtg ttatatcaaa atgtgctgcc ccgaaaatac ttctgtgcag
     3961 acatattgtg atctcagact tttccctgac atcattcgta tatgacaaat gtcagtgtat
     4021 gttttacttc ttctgcgtca tctgcggtat cggaagcttc gtttcctgct gcggacttac
     4081 tttcggattc tacttcaaac tggcctttct ccgcattgcc atcggatttt gatccgccgt
     4141 ctgtgctctc actttccgat gctgtctccg tctcggattc tgctgccttt tcccgctctt
     4201 ctgaaatgct ggttgccgtc ttttttccgg tgacaactgc atccgtcgta acgtaaagga
     4261 tcttgcccgg aacttcgata tccatctctt ccgaacagat tacaacgttg tagttcagac
     4321 cgcccaaaat atcggatctt gtcacacttc ctgtaacttt tccgttttcc actttctgaa
     4381 attcatccgg gaattcgtac ttctgatcgt atgctccctg caggtctccc gtataaaagg
     4441 tcacattgtt gaaatcctga taatccctgg aagccgcata gttcatatat agctctgctt
     4501 ttccgtctgt gacgttaaat tttttgacaa cgatcggatc accttcctgt gactgattgt
     4561 acgcactcac agaatcatcg atcgtctgct tcagctcatc ctcgctgtag taggatttgt
     4621 ccagcgtgtc gatcaccgcc gcctgtacac tgccatcccg tttcactttt actgcattgt
     4681 gctctgcctg aaactgactg cagccgccaa gacatccggt tatcaggatg actgccagaa
     4741 ttcccttcca tttcttcata atatttccca tccagacaca gcaaaatctc cttcgctgcc
     4801 ctgcctttct tgaaacttac catataccct tctgtatata aaatacagaa tacgccactt
     4861 gttcgcatgg catttttcat cataccatta ttgaccgtcc ttgtaaagag cgtttctggt
     4921 atgccgcatg atctccagaa tctccagaga tttgaaatcc cggtctatgt aacgccttcg
     4981 gaatttctca accagactga taaactcttt ctttacctgt gctgatagtg caaaggcaaa
     5041 gaggcgttcg accggcgtac tgatcacata ttgcatcgta tagatcgttg attccagaac
     5101 cggtgtgttc ctttgtactt ttgagacaca gctgcgacac aatacgctgt tcttatctgt
     5161 aaggaaggca caaagatcat gctttgctcc acatactgca cactcgaaca cttgcggata
     5221 ttctccgcct gccgccatca tacgcagttc aaaggcataa cggatcagtt cccggtccag
     5281 tttttgattg agcactgcac gcagtgcccc gtagagcagc tggatgatct gtgtgccgtc
     5341 atcgttctct ctggcataat aatcggcaaa ctccagaaaa tagcttccat agcaggcagc
     5401 ctccacatct tctgtgatct cccggaaata atttcgaatc tctgtctgca caagctgata
     5461 ggcagtcctt gcttctttta cataaaaggt tccaaaagca aaggtgtttg cggcagccag
     5521 aaagggattg cccggtcttc tggctcctct tgcaaataca gtcacctttc cccgctctct
     5581 tgtcagcagc accagccgtt tatctgattc tccgatcggt gccgctataa gcacaatccc
     5641 tgtcagtgca attttatcat ccatatattc tctcatattc ttacagtccc gtcagcaaat
     5701 gctgatgtct gtacggaatc gtcgtttctg atttcagata tcttttttat cgtatccgaa
     5761 gtttttgatc atgaagtcac tgtctctcca gtctttcttg actttgaccc agagctggag
     5821 attgactttt tcttcgagca tatcctcgat atcacggcgt gcctgtgaac cgatcctgcg
     5881 gagcatctgt ccgccttttc cgatgatgat tcctttgtga gaatcccgct cgcagatgat
     5941 cgtcgcctcg atatcacaga tgctgccgcc tttgcttttg cgttctgtca ttttttcaat
     6001 agaaacggca ataccatgcg ggatctcctc ttccagagag cgaagtgcct tttcgcggat
     6061 catctctgcc acgatctgtc tctgcggctg gtctgtcacc gtatcttcat cataaaactg
     6121 cggtccgtac ggcagatatt tgaaaatact ctcgatcaca gtatccatat tgacactgcg
     6181 aagtgccgac accgggatga tctccgcaaa atcatagact ttgcggtaag catccatata
     6241 ttttatgatc tcttttttat cta
//
LOCUS       DAWDWH010000118         6257 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5485_length_6257_cov_11.579652, whole genome shotgun sequence.
ACCESSION   DAWDWH010000118 DAWDWH010000000
VERSION     DAWDWH010000118.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6257)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5485_length_6257_cov_11.579652"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5037..5046
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1466 a         1628 c         1268 g         1885 t
ORIGIN      
        1 ttcgcaatac agacagactt ttcctgtttc cagtgatttt ggcacatcga ttacccgctg
       61 attgctgctg cctttccagt gaagctgcac attgcgtttg tctttctcat attcgccgtc
      121 tacacagaca tccacatact gcatcacttc aaaatggctg atctgttccc acagatatcc
      181 agtgtacagc cagattgttt tcgttggaaa tttttctctg atctcatgca ccagtgcacg
      241 gatatccaga cggtttgccg gatgcagcgg atctccgccg cttaaggtaa taccggagac
      301 atagcttttg cccagttcct caaacagctc gttttttgct tcctggttaa acggaatgcc
      361 gccatttgga tcccaggtga tcggattctg acattcctca cagcagtgat tgcagccgga
      421 aacccagagt acaacacgca atccgtctcc gtttaacata tcgtcctttg ttatattatg
      481 gtatctcata ttgtctcctt gcttcttatc atacataaat ttatcttagt cggagaagac
      541 gcccacttct atagacggga atcatctcac atctgatata cgccaaaaaa ctgccacttc
      601 aaaaaaaagt ggtagttttc tgtatggatc tatgttcaga tgaagaactt gtaagtgctg
      661 tttctacctg tcacttagtt gccgcactcg atactgatct cattgaatag atcaagcaca
      721 tcatctacat tcatattttt cttctcttcg ccctttttgt caaggattac ttttccctga
      781 tgcatcatga cgatacgcgt gccgtattcg atcgcatagc gaagattgtg ggtaaccatg
      841 atggatgtga gcttcttttc acgcacaatc tgatccgtca gacgcatatt gatctcggca
      901 gtctttggat ccagtgcagc cgtgtgttca tccaggatca ggaaatccaa cggtgtcatc
      961 gtggacatca gaagtgacat tgcctgtctc tgtcctccgg acagggctcc gacttttaca
     1021 tgaagcttat cttccagtcc gagattcagc ggacggagca actcgcggta agcttcttct
     1081 ctttttttat tgacaccctt tccaaggtta taggcttttc ctttattctc tgccattgcc
     1141 atattttcaa ggatcgtcat ggacgggcag gttcccatgg aaggattctg aaaaacacgt
     1201 ccgattcttc ccatgcgttt atattccggc atatttgtga tattggtgcc gttgatcagg
     1261 acagctcctt catcaatcgg aatgctcccg cagataatat tcagcatgga agtttttccg
     1321 gaaccgttgc tgccgacaac cgcaacaaac tcaccgtcat cgactttaaa attaaaattg
     1381 ttgaacaggc acatttcatt gactgtgcca ggattatagt atttataaat ttctctgagt
     1441 tcaagcatcg actttcacct tgaccttcct gtttcgttcc attccaagca ccaggatcag
     1501 caggaacaat gccgctgtga taaatttcag gtccgtggac tcaaaaccaa aacgaatggc
     1561 gattgccaca catgctttat agatcaccga accgattacc acgcttgatg tcacacgcag
     1621 aatcgttact ttacggaaaa ggctggttcc gatgatcaca ctggcaagac cgatgaccat
     1681 cgttccagtt cccatggata catcgaaata tcgctgttgc tgtgcaaaca cacagccact
     1741 taatgccacc aggccgtttg agatcgcaag acccaccacc tttacctttc ctttgtttac
     1801 accaagggat gtaatgatat gatcattatc cccgactgca cgcagcagca taccggattt
     1861 cgttctcaga taccagtcca gaatgtattt gctgatcagg gtgatgatca gaacaatgat
     1921 cactacctta aaagaagaaa ggctttttgg aatgatttta tctaccagat cattctggaa
     1981 aatggtactt ttattgtaga tcggcaggtt tgccctgcct gcgattcgga gatttaccgt
     2041 ataaagggcc gtcatcataa tgataccgga cagcagatca cgcactttta attttacatg
     2101 gataaggcct gtcaatgttc cgactacagc cccgagcaga aaacagattg gcagtgtcag
     2161 cagcgggttt acatctctgc tgatcagtgc tgctgcgatc gctgctccca tcgggaaact
     2221 gccatctacc gtcagatccg gaaaatccag gattttgtat gtaatataaa ttccaaatgc
     2281 catgatcgca tatatcagtc cctgttccag gacgcttatc agcaatgcca tatcaacctc
     2341 cgattattcc actgtgatct catcgaaaac ttctgccgca ccatctacgg tagcctgatc
     2401 cagctcaaag ttcagtttgt cagcaactgc ggtatttaca taaaggcttg caccgctgca
     2461 tacttcatac ggcatttctt ctgcctttga ctcacctttt aaaatcttgg ctgccatctc
     2521 gccggtctgt ttgccaaggg cgatataatc aatacccata gatgccagac atccgctctt
     2581 aacctgttcg atttcagaac caaatacagg gatattcttc tcatctgcgg ctgcgatcac
     2641 ggtctgcagt gcagatacaa cggtgttgtc tgtcaggttg ctgatgcagt ctaccttgga
     2701 tgccagatcc tttgcagcca tctcaacatc tgctacggta ttgataccgc tgtcaacaat
     2761 ctcaaaatca taattctctg ccagctcttt gtattctttg atcgtgctta cggagtttgc
     2821 ttcgcttgtt gtgtaaagaa taccgatctt ctttgcatcc ggcatcagat cacggatcat
     2881 tttcagctgt tcttcaaccg gaagtttatc agaggttccg gtgatatttc ctacggaatt
     2941 gccctcttca tctgcaagtc ctgcaccgac cgggtcagaa actgcggtat atactaccgg
     3001 gatctcactg tccagacagc tgttataggc actcatggca ctcggagttg cgattgcaca
     3061 gatcagattc acatttttgg atacaaagtt atcagcgatt gtacttgcgg ttccggtatc
     3121 tgcctgtgaa ttctggtatt ctactgtcag attgacacct tcttcgatcc cagcactttt
     3181 taaaccttcc aggaatcctt cgcggcagtt atcaagagaa ccgtgctctg caaactgaga
     3241 aataccgatg gtataatcac tttctgccgc actgctgtct gcggatgttt cacttctggt
     3301 tgatgcctgt gtatcttttg cagtgtctga actgccgcat cctgtcatca ttgccataat
     3361 catcgctgtt gtcataatac ctgctaatac ttttttcttc ataataaaag tcctccttct
     3421 tctgccgtga aacagttaca attttctgat aaataaaaaa acgcctcaag cttttctgcc
     3481 tgagacgctg atattcatca acgcggtacc actcaaattg acttctgtcc actttgcatg
     3541 tacatagtta tacattccag attgataacg ggttcggttc ccgacaacgc ctactctcct
     3601 gtccggattt tgggctgccc tcgaaagtcc attcaatcat ccacgccgta ctgcaatccc
     3661 accgtctgca gctctctgtg cctttatgga aaatctactc ttctttctca tcggtttttc
     3721 ctatgcaatc aatagtaata ctttttcgct ttcttgtcaa tgaaaatttc tgtttttaat
     3781 ccactgtgat ctttctgtgc aaagaactgc acagaagaat cacgatcaga tcagagatca
     3841 tttgacgggt gctgatctct agcccagcct ccggcacagg aaacggcttt tttccatcct
     3901 tttcggactt cttttcgcca gttttcttcc tgctttggca aaaaggtatg ctccagcgcc
     3961 cagttttttc ggatttcttc gctgtttttc cagtagccga ctccgatgcc ggcaagatag
     4021 gcagctccaa gggctgtcgt ctcgatgcat ttcgggcgtt ctacttttgc attcagaaga
     4081 tcggcctgaa actgcatcag gaaatcattg gcacaggctc cgccgtccac tttcagggat
     4141 gcaagtgaca gacctgcttc tttttccatc gcttcgatca catccgtgac ctgataggcg
     4201 agtgactcta ccgttgcccg gatcagatgt tcttttttcg tgccgcgtgt gatgccgacg
     4261 atcgttcctc tggcatatgg atcccagtac ggtgcagcaa gtccggtaaa tgccgggacg
     4321 acatagatac cgttcgagtc cttcactttt ttgcagtatt cttcggattc tgccgcatga
     4381 tcgatcatac gcagatcatc gcgaagccac tgaatggctg ctcctgccac aaaaacgctg
     4441 ccttccagtg cgtactgtat ctgatcgtca actcctgctg caatcgtagt cagcagtcca
     4501 ttttttgaga tgaccggcca gtctccggta ttcatcagca ggaaacaacc ggtgccatag
     4561 gtgtttttta cgtcaccgga gcgaaaacag cactgcccga acaatgcagc ctgctgatcc
     4621 ccggctgctc cggctatcgc aataccgtca ccgatcagac agtcttttgt ataaccgtag
     4681 acaccactcg atggttttac ttccggcagc atttcgcatg gaatatccag tttttgcagg
     4741 atttcttcgt cccattttcg tttatgtata tcaaagagca tcgttctgga tgcattggtg
     4801 taatcggtga catgtgcctg cccgcctgtc agtttccaga tcagccacgt atctacagtt
     4861 ccaaacagca gttcgccatt ttttgcacgt tctctgcctt gttcgatatg atcaaggatc
     4921 caggcaagtt tggtcgccga aaagtaagca tctgggatca gtcctgtttt tttgtggatt
     4981 ttttcaccaa atccgtcttt taccagttcc tccacttttt ctgcagtcct gcggcannnn
     5041 nnnnnnttgg taatgccgat cgccgcaatg ttcttttccg ttacaccgat cttgcccatc
     5101 gccttggctg ctaccgcgat ctgcgaagac caaatttcca tcggatcatg ttcgacccat
     5161 ccaggttttg ggaaaatctg cgtaaattcc atctgtgcca tactgcagat gtttccctgc
     5221 ctgtcaaata aaatgcaccg ggaactcgtg gttccctgat ccagtgccat aatatagttc
     5281 tgcattttta cccccatttt atcttagtcg gagaagactc ccacttctat attaagtccg
     5341 gaaatccgtc tctcgctaag accgattttt tatttgcctt ttattataca tttggatcta
     5401 ccaaaaatcc agtaaaaaac agggattcat tcatcccgga acagacattt tttcatatct
     5461 tccggaaacg gtgcttcaac ggtgatccgc tttccctcaa atggctgata aaaacagatc
     5521 ctgaatgcat gaagtgccgc ccgtttgatg ccggtaaagc aattctctgc atccacatct
     5581 gctttttgat ctgtgtccgc aaaatacagc cgatctccaa tcagcggatg ccctgcatct
     5641 gccatatgta cgcgaatctg gtgtgttcgt ccggtttcca gggtaagctg caccagtgcc
     5701 gcttccatat actgtttctg cacctgataa tgtgtgactg ccggtttgcc gtcttttgaa
     5761 acacacattt tcatcaggct tccgggcaaa ggtgcgatcg caccgtttaa tgttccgttc
     5821 ttttctgcaa atatgccgct gcagatcgcc agatatgttt tctgcaacct gccgtcttct
     5881 ctttgtctgg agagtcttgc cgctgccgca cggtgttttg caaacagcac gatgccgctt
     5941 gtctccagat ccagcctgcc tatgctgtga atttccacct gctgagtgtt cttccgaaaa
     6001 taataggcaa gctgattggc cagcgtatcc tgataatgac catgcgacgg atgtactgcc
     6061 ataccggctg gtttattcac tgcgatcaca tccgcatctt catagagaat gtccggcatt
     6121 tgttcaaagg acagcagatg tgcggagctg ctgttttttg tttccagcaa aacttccagc
     6181 acatcgcctt ctgacagtct ggtcgccact cttgcccgct gcccgcccaa acagatgcca
     6241 ttttctgtaa atttcat
//
LOCUS       DAWDWH010000119         6173 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5553_length_6173_cov_11.697614, whole genome shotgun sequence.
ACCESSION   DAWDWH010000119 DAWDWH010000000
VERSION     DAWDWH010000119.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6173)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5553_length_6173_cov_11.697614"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1432 a         1678 c         1173 g         1890 t
ORIGIN      
        1 ttcttcgccg tcatatgcac agtcaacttc catgccgtcc tgctccaggc tgaagcggat
       61 tccttttacg atcagtttct catcatctac aactaatact ttctttgcca tttttgttca
      121 ttccttttgt tttattttac tgctgtactg ttattttatc ttttattttt ccaaacaccg
      181 cttctttgat cccgctgata ttcataatat cttcgatctg ctcaaatccg ccctgttctt
      241 cccggtaacg tacgatatcg gcagctttgg atgccccgat gccgggaagc gtcatcagtt
      301 cttcttctga tgcctgattg atattgactt tgcctgcatc tgccactctg ccctcaccgg
      361 acgtttccag agaaccctcg gaagatgaca gctgctttgt cttctgttct tcgactgtaa
      421 cgatatacag ttgctgcccg tcctgaagta cggatgcctg attgaccgct gagatggcag
      481 catcttttgc aaacccaccg gcaagctcga tcgcttcata cacacgtgct ccccttgcaa
      541 gttttacaac accgggagtt ctgacggcac ctgccacatc tacatagatc caggcgtctg
      601 tttccacatc cgtcccccct gctttcatcc gctccgtttc tttcgattct cccacttcag
      661 actgattgga aatttcttcc gaaaacacct catccgccgc ctgttcaaac gtgatctcac
      721 cttttttatt acagccgccc aaaaaaatac acagtacaac gattatggtc agataaaaca
      781 gactctttaa ctttctgcct gattttctca aaaggactcc ttcctgcctt ctgacaaaat
      841 tcccctgtac agatattcct attttatcga acattgttca aaaatacaac cgttattttt
      901 caaatatgac aacaccgatg atcgcgatcg ccatccctgc cacttttcgc atggtaagcg
      961 gttgtttttc tacgccaaac catcccagaa actcgatcac gtaggcaatg atcagctggg
     1021 agatcacgat cagcatcgcc gctttcgcag gtcccagact gttcatgctc tttattacgg
     1081 taacggtgat aaatgcaccg atcacacctc caagcagcgt ccatttatcc gggacctgtg
     1141 taagcgtcat aaaacttttc ccgtccgaaa aataccagat cacaagacag accagaaatg
     1201 ccgtaaactg cacaaaactg gtcgaaaccc agaggctggt cgcctttgtc acctgcgtgt
     1261 tgaagacgcc ctgcacactc atcagggctc cagataaaag tgctgttaaa attccaatca
     1321 taaaaaaact cctttgcctg tactttctga cagtatatgc acaaaggagt tttctataca
     1381 ttttttactg ttttttcaag tcgaacgcac gtgagacttg gcacgcgatt ctggtcagct
     1441 ttgctgacat ttacccaaca gaatcgctgt gcatcctcgc ggagcgttta tcttagtcgg
     1501 agattgcact cccactaaga caaatttgtc gcttgccact tatagaagtt tgagtcttct
     1561 ccgactaaga taaaaccgct tccagttttt caaccatctc gtcgatgtct ttctcggtga
     1621 tcacaagcgg aggcaccaga cgcagtacat cacctcctgc ggaaagcaca atcagaccat
     1681 ttgccagagc tgctttgaca agttcgccga ccggtctgcc gctcacaacc agtccctgca
     1741 tgaatccttt tccgcggcgt gcggtaaagc agtcatgttt ttctaccagt tcatccagtt
     1801 tcttttccag atacggagct gtctttctta catgaccgac gatgtcatgt tcttcaaaca
     1861 gatcgaacac tttggaaacc gctgcacagg cgaagggatt tccgccgtaa gttgtaccgt
     1921 gatctcccgg aactaaagag gcttttgcag tcttttcgtt taagacaaac gctccgaccg
     1981 gcacgccgca tcccagtgcc tttgcacagg tcatgatatc cggcatcacg ccgtactgct
     2041 gccatgcaaa catgcttccg gtacgtccca taccacactg gatttcatcg aggatcagaa
     2101 gaatatcttt ttcttcacag atttctttaa caccttttaa gaagctctct tccatcggat
     2161 aaatgccgcc ttcgccctga agggtttcca tgataatcgc acaggttttg tctgtgatct
     2221 gagcttttac actgtccaga tcattgtaat ctgcaaattt cacgccgccc atcagcggtt
     2281 tgaacgcttc ctgataatgc gggtttccgg taacggaaag tgctccgacg cttcttccgt
     2341 ggaaggaatg gttcatagca atgatctcgt gatccgttgc atggtctttg ttccatgcat
     2401 atttacgtgc ggctttgatc gcaccttcga tcgcctccgt accgctgttg gtaaagaata
     2461 ctttgctcat gccacttgcc tttaccagtt tttctgctgc ttccatcatc ggcacattgt
     2521 agaaaagatt ggaggtatgg atcactttgt cgatctgatc ttttaaagcc tgatcgtagt
     2581 ccgcatagtg atagccaagt gcaaacaccg caattccggc accgaaatcc agatattcct
     2641 ttccttcggt atcataaaga tgtacgcctt ctcctttttc aaaaacaacc gggaaacggt
     2701 tataggtgtg cagaaccacc tgttccgttc tctccatata ttcattcatt ttcattgtaa
     2761 aaacgttcct cctcatcccc gataatcgca gtaccgatac ctttgttggt atagaactcc
     2821 aacagcaggc agtgcggaat ccttccgtcc aggatatgta ctctggaaac accggacttg
     2881 atcgcatcga cacagttctg gatcttcggg agcatgccgc cgccgatatt tccgctgccg
     2941 agcagttctt ctgcttccgg aatactcagt tcggagatca gtgtggattt gtcctgcgga
     3001 tcacggtata caccctcgat atccgttaaa aataccagtt tggatgctcc gactgccgtt
     3061 gcaatcgcac atgctgcatc gtctgcattg atattataag aatgatactc atcatcggaa
     3121 ccgatcgggc aaatcaccgg gacaaaatca ttgtccagca gtgtatttaa aagctcggta
     3181 ttgacttttg tcacttcgcc tacaaaaccg atatcttcgc ctttggagag tttcttctta
     3241 acacgcagaa cggagccgtc ttttccgctg ataccaaccg ctttgactcc gactttctcc
     3301 atcatagaca ccaatccttt gttgatctta tttaagacca tctcagccac ttccatggtg
     3361 tctttatccg tcacacgcag tccgttgata aatttggtct caatgccgac tttgcctgtc
     3421 cattttgtga tttcttttcc gccaccgtgc acgatgatcg ggcgaaatcc gaccagtttc
     3481 agaagtgcta cgtcacggat gacgtttttc ttcagttcat gttctaccat ggcacttccg
     3541 ccgtatttta caacgatggt cttgccgctg aatttccgta tataaggtaa cgcttcattg
     3601 agcacactgg ctttctgaag cagatcctgc attgtatcca ttgtttttcc tccagtttct
     3661 atccatactt ccggcatccg gacagatgcc tgtaaattgc tctgccacaa agcagctctt
     3721 accgcttaag agcggtaatc ggcattgatc tttacataat cgtaagtcag atcacaaccc
     3781 caggcagatg cggatgcctc tcccattttg atgtcagcaa cagccgtcac tgctttttct
     3841 gagagtacct ttgtcgcttc ttcttcgctg taatcaagtg ccacaccgtt ttcgatgatc
     3901 ttcagcttac ctgctgcact ttcaaaatac agatctactt tttccggatc aaacttagct
     3961 ccggaataac ccatggcaca gaggatacgt ccccagtttg catcatgtcc gaaaatagcc
     4021 gctttggtaa ggtttgaaca aataacggat tttgccagaa tcttcgcctg ttcttttgac
     4081 tgtgctccga tcacttttgt ctcaaacaat gcagtggcac cttcgccgtc ccctgccatt
     4141 ttctttgcga gcgtctcgtt gatatagtgc agtgccctgg caaatgtctg ataatcttcg
     4201 ccttcctctg taatttccgg attctctgcc agtccgtttg ccagcagcag aacggtatca
     4261 tttgtggaag tatctccgtc aacagaaatc atattgtagg tatcatcgac atctgcactc
     4321 aatgccttct gaagcatttc ttttgaaata gctgcatctg tcatgaggaa gctcagcatc
     4381 gtgcacatgt tcggatggat cataccggaa cctttgcaca taccgccgat cgttaccgtt
     4441 ttaccgccaa tctcaacttc aacggcaacc tcttttttta ccgtatccgt tgtcatgatc
     4501 gattctgctg ccagtgttgc tgcttctctg gtatccagaa gcagtccgga aagatccttc
     4561 acgccttttt tgatcgtatc gattggcagc tgttttccga tcacaccggt agatgctacc
     4621 agaacctcat ccttgtcgat accgagtgct tctgctgccg cttttgcagt ctcttcacag
     4681 tagccatatc cttctgctcc ggtgcaggcg tttgcaatac cgctgtttac cacaacagct
     4741 ctcgcctctc catgttcgta taccacatgc tgatcccatt ttaccggtgc ggcttttaca
     4801 aggtttgtcg taaacgttcc ggctgccata catggtgcac tgcttacgat cattgccata
     4861 tctttttttg tacctttttt gatacctgca tgcataccgg ctgctttaaa tccttttgca
     4921 gcggttacac ctccatctat tatcttcatc tatgctcacg acctttctct gttttatggg
     4981 aacattggta cgccctgaag gccttcgttc tcggcaaatc caaacatcag gttcatattc
     5041 tgcacggcct gaccggctgc acctttcacc agattgtcca ttgcccccat catgatcaca
     5101 cggtttgttc tcggatccag tttgaagttt acatccacat aattgcttcc ttctacccac
     5161 ttcgtctccg ggcagacatc cttatccaga acacgtacaa actgttcttt tccgtaataa
     5221 gcatcataga tcgccttaac ttcttcgtaa gtcacctctt ttttcagaga tgcataagca
     5281 gtaaccagaa taccgcggtt catcggaacc agatgcggtg taaaattaat atggatcgga
     5341 taaccggcgg cataacctaa ctgctcttca atctccggtg tgtgtctgtg tgttgcaaca
     5401 ccgtatgctt tgatgttctc attgacttca cagtaaaggt ttgcaacctt ggcactgcgt
     5461 cctgctccgg aagtaccgga tttagcatca atgataacgg tatttccatc aataatgcct
     5521 tcctttaaca atggataaat ggacaaggtg ctgcatgtcg gataacatcc cggatttgcg
     5581 atcagtcttg cctttttgat atcctcccgg ttgatctcac acagtccgta aacagcttcc
     5641 tcaataaact gcggtgcttt gtgttccaga ttataccact gctcataagt cttccggtct
     5701 ttgatacgga agtccgcact tagatcgatc acttttgcct gacttaaaat cccttcattt
     5761 ataagagatg cacacaaccc ctgcggtgtt gctgtgaaaa tcacatctac ctcttttgcc
     5821 agctgctcca tattgtcatc cagacatttt gcatccacga tctggaacat attctggtaa
     5881 acactgctgt atctctgatc cacataactt ctggaaccat accacgcaat ctctgcttct
     5941 ttatggttaa ggagaagacg gacaagttcg cctcctgcat atccggttgc tcctattatt
     6001 ccggctttta tcatatcaac attctccttt caacctaata tatcttttct gtccggatta
     6061 ttcttctttt ctttttctgt caaatgtgag gtgccgccca tctgatgcag tctccggcag
     6121 attcataaaa attcgctgtg cgaatgaaat ctttcttatc atataccagt ttt
//
LOCUS       DAWDWH010000120         6170 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5556_length_6170_cov_11.450368, whole genome shotgun sequence.
ACCESSION   DAWDWH010000120 DAWDWH010000000
VERSION     DAWDWH010000120.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5556_length_6170_cov_11.450368"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1674..1793
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1554 a         1590 c          979 g         1927 t
ORIGIN      
        1 catccaccta tgagcaagct catgtggatt tagacctttt gaccctggca tcatatcata
       61 ttatttcagt cgaatattga aggagtatag ctatgaaata tcagaaatta ttaagtctca
      121 cccgtcaggc agtcgatact taccagatga tcgacgaagg cgaccacatc gcagtcggca
      181 tttccggagg aaaagacagc ctgacgcttc tgtgtgcact ctatggcctg aaacgctttt
      241 atccgaaagc gtttgatctg actgccatca ccgtgaacct tggtttccag aatcttgatt
      301 tggagccaat caaaagaatg tgccggacgc ttgaaattcc ctataaaatc gtggatacac
      361 agattgcccg gatcattttt gaagaacgcc gtgaaaacaa cccatgttct ttgtgtgcta
      421 aaatgagaaa aggcgctctc aatgctgcca tcaaagaact tggctgcaat aagatcgcct
      481 acgcacatca taaggatgat gtgatcgaga ctatgcttct gtctcttctt tatgaaggac
      541 gcttttactc gttcccaccg aaaacatatt tagatcaaat ggatctgact gtgatccgtc
      601 cgctgttttt tgtcgatgaa gcggatgtga tcggattctg gcataaatat cagctcccgg
      661 ttgccaaaag tccctgtccg gctgacggac atacacgccg cgagtacagc aaacggcttc
      721 tgcgtcagtt gaacctggaa aatcctggtg tcaaaaaccg gatgtttact gccattctca
      781 atgcggaact ttctgactgg ccggatcgta taatccctga ccgccactga tggaaagcgg
      841 aagatatttt cgctgctttc tgcgtttgtt gtatttggca aaaccggcca ctttgttgta
      901 gacccagaaa gaatacaaca gcagatcgtt cgccttgccg attttttctt tggtcgtacc
      961 atgataatac gtgccgcctg ccttgatcgg tgctctgcgt ttcacttgct cgatcagatc
     1021 ggattctgta tggatcggtt cacgaaattc tgtatatgcc gttccaatgc agtccgcttt
     1081 gtgagaatca ctgccgccaa actctgccag aaagtacttt ctggccgcct tctttgcttc
     1141 ctgattccgt tcttccactt cacaggaatt gtatgcctca aggaaatcaa actcctgcat
     1201 gatcttcgga tttcgctgat atttcttaga acttccaaaa cttaaatatt tctctccaaa
     1261 cggatgtgcc ggtccgagaa ttcctccgta agcatgtacg accctggtca gcaattctac
     1321 cggcatcccc cgcatttcca aaatacgcgg ctttacacct tccggcagaa ttaccagaaa
     1381 atgtcctgca ttgtttgtat catactcaat tcccttcagc actacgaagt ccgtatactt
     1441 ttttcctttg atgtgatctc tccagtatct gtaaccatta taagaattat gatcagatac
     1501 caacattccc tgataacctt tttccttcaa aatgcgaata tattcttcaa tttcgacttt
     1561 tccatccaaa gatccctctt ttgtatggca atgcatatca aatttcacaa gaaactcctt
     1621 cctgaaacgc tctttgtact gaaaaaccat attcacagaa cgctggtttt tcannnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncttcttg
     1801 cctgtctgcg acgtgcatga cgttccttct gttcatctgc ttctcttttt ctgtgacttt
     1861 ttttctctct cttatctttt cccttttctt ctgtgacatc cgatgtctct gaaatatttt
     1921 ctgccggttt ttcaagcaga aaaacactgt agcctgctgc cagtgccgca agtgatgaaa
     1981 ccggatatac ccaccattct tcccggtgca gcagcgtctt tataacaaaa aacatcagta
     2041 ctgcacagac cagaaacagc cccgcccggt aaaaccgaaa agccagtata cccaggatca
     2101 ccgcacagac tgcaccgatt ccgagacaca gtctcacatt tgcgaacagt acacggctca
     2161 gctgatatcc gacaaccatc cccgtacatg ctgctattac acagaacagc agctttcgaa
     2221 atccctgaaa tatgttcatt gtatgtttta ttcctccgtt cttctgacac ttcccttttc
     2281 tcttgattga ataagcttcc catttctata tcacagcaga aaatccatcc aaccgaaatt
     2341 tttgttttct actattcttc tcctgctaag agtatagcac agctgcctgc tttttgctac
     2401 ctcaatccct caaaaagcat ctggactctt ttcccttctt tttcaagcaa aaaacatccg
     2461 ttctttcctc agattttctc tgattcttaa gaacggatgc ctttcgcttt ttatttctta
     2521 taccactttt tattttattc ttctgctgtc tcttctgtct ctgtctccgg tacaacatta
     2581 tgtacggctg ttaccgttgc cacaactgct tccggatcgg ttgacagatc aatctcttta
     2641 tcttttgcaa tctccagatc ctttacacgg atcgtatcgc cgactttcat atttccaaca
     2701 tctactttta ctttatcaac cagtgctgcc ggcaatgctt tgtatgcgat ctcacttaaa
     2761 ccttcctgta caacaccgct tgcaactttc tctgcattta ccagaatgac ttctgcaacg
     2821 gaatgaactt tttcattgct taccagagcc tggaaatcaa tctcatccac tcttcttgcc
     2881 attgcattgt actgaatgtc tttgatcagg gcatcatact gtttgccgtc tacttccagc
     2941 ataacctgac tacctttgtt atctactttg agcaactgct ctacatcttt cttactcagc
     3001 ttcagcatga tagaattttc tatctctctg ccaaataagt taccagtaac aaatccttct
     3061 cttcttagtt tctttgcttt ttcatctgtg cttctttttt cagcttttaa agtattcatt
     3121 tatcttttcc tccaaaaatc tgatttctta gcagccttca acaaagaggt tttgttaagt
     3181 tctttcttgt tacgcctata ttataatcgt tatataacaa aacgattaac caaaaatcaa
     3241 attcttttcg gctcttattt gtgcattttt ttcatatttt ctgtaaagcg gacattcaca
     3301 atccgcttcg ctacttgctc accgcttagt gctctgcaca cttgaagcgg gggaatggtt
     3361 ctctgcgttc aaaaaatagt acaattcttc ggaagcatcc ggatttattt ctcttttctg
     3421 agcctccagc atccgtttta cttttttttc atcgtggatc agacgcacac cgcttgccac
     3481 ctgaccaatc gctgtctttc cggaaacgct cattccaagc atccggaaaa atctcctgtt
     3541 ttccgtctca catcccggaa tcggccggat atgcacgatt gggataccac tgactgccgc
     3601 ttcggtagaa gtcagtcctc ccggtttcgt aaagatcaca tcacaggctt tcagataaag
     3661 aggcatcttt tttgtgtaac cgacaagcct tacctctttt ctgtttttat atttctgctg
     3721 aagctttttc tttaactttt ggttgcttcc acagatgacc acaagatttt cctttttgct
     3781 tcgcattgcc agcaaaagtt ccaggatatg cacgattgct tttgttccca tacttcctgc
     3841 cgccagcaga aaatatttct tttcctgctc cagtccaagc atatcgcgtg cctgttctct
     3901 ggaaatatcc tcccggcact gcctggatac cggaatacca aacgctttca gtttatatgc
     3961 cggaattcct ctttttatga tctccggatg cagtttttta tgcggtgtaa tataatagtc
     4021 acagtcggtt tcttcccaga acgggataca ggtgtagtct gtcatcacgg ccaccgtaag
     4081 cggcaaagca aacccatttc ttcgcatata agtaatcgtt tctgccggat aaagatgtgg
     4141 cataacgatc gcatccaccg gattttcctg aagatattct tccagatagc gtgccattct
     4201 ggcatttgca taatacaccg gagatttttt catcagtcga cttaccacca ttccaagttt
     4261 atacgccatg ccaaacatgg ccggtgcttt tttaacaacc ttcacataac catccccgac
     4321 cagtttcgat actccatttc cggcaagaga cagataatcc agcacttctg cacagtcacc
     4381 atgctcacgg attgcttctg cgatcgcctg tgccgccgca ttatgtccgc cgcctgtatt
     4441 acaggataag attagcactt tcatttgatt tctcctccat cagatgatgc ttatagatca
     4501 caatacacga aatcaggaca catcctaaaa gaatcgcagg tatgggaacc agagacaggc
     4561 agccgacaca aaaacagcca aaagtcacaa ttgaacgtct gcgatgtgaa cggatcttaa
     4621 aaaccaccga aaataagata taaaaaaacg ccagcaaaag caccggtttc cagattggaa
     4681 acaacgccag cagtacccca aaagaaactg caattccctt tccacctttt cttccgtgaa
     4741 aaattgagaa ggcatgtcca aacaccggtg ccgccatcac aagagcaaag atcagctcct
     4801 ctgtactata attctgacga tacagtatca ccggcaaata tcccttgccc atatccgcca
     4861 gcaatgtcag aatcccgcac cagaatccgc caaacacaaa tgcattatac gttcccggat
     4921 tttcatcctc tgcaagtttt ctgatatcaa cacccttcaa cagccatgtg atgcgatatc
     4981 caaacaacat gctccccaaa aaatatccaa gaaaaacata tgccagttgc atccttcttt
     5041 tcctccagcc tctttttctt attcgaaagg cagttcaaaa gaaatatcgc tttccatatc
     5101 ttccgaatat ccctctaact cctgttcgta tttctgcaga gcttccgtaa ctgccatccg
     5161 ctccttttcc tttccgttta caagatacag atcagccgcc gccataaaca gaatatcatt
     5221 ctcatctgta caaggcgtat cttctgaaat atattttctt acaacgtcct ccgccccttc
     5281 cagatctttc agggtcattt ttgcgtacac aagagctgtt gcagccatct catgctgatc
     5341 ttctgcatac catttctcac agaatttcag tgcttcttcc tttttatctt ccgcagccag
     5401 agctgaagcg atccgaaact tcaaatcccc cggatatact tcctgccact ggaacagatt
     5461 caacagttcc tcacatcttt ccattaattt ttcatgttct tcccttgcat ccagctcgtc
     5521 cagataatcc tcgatccagt ctgccacacc atacgcaaaa tccgtagcat catccatttt
     5581 caggatctca gctgcatact gcggatcttt ttctcttccc tgtgcaacaa gttgtttgaa
     5641 gacttcataa gcctcatccc actgaggaca gttatcctcc agctcactca tataacaaat
     5701 ttccgtcaat ttatcaaatt ttttccataa ttgcttcatt ttcttctcct gttctttttc
     5761 atagtttgtt tttatgtttt attcacgttt ccattttctt cattttacac atacagtatt
     5821 tttacgtcaa gaatattcgc cgtatatttc ttaacaaatc tatctctcat tttactttta
     5881 tctctcatct tcctgcaagg cgatttttat cagcataagt ataaaaatag cacgcatctc
     5941 tgcgtactat actcctttaa tcaaatacag ttattttcat aaattctatc atatactttc
     6001 tgagcttctc gtccaaactc attggaatca taataatctg gtggtgcaaa tccttttcta
     6061 tcaatacaat ccccaatagc atccaatatt tgtctgacct tcccctgaag aatccattta
     6121 tcaatatctg ttatattatt ttctaaaaaa atcctgtctc tatcacttaa
//
LOCUS       DAWDWH010000121         6099 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5618_length_6099_cov_15.670748, whole genome shotgun sequence.
ACCESSION   DAWDWH010000121 DAWDWH010000000
VERSION     DAWDWH010000121.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5618_length_6099_cov_15.670748"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2172 a          904 c         1428 g         1595 t
ORIGIN      
        1 aatgataagg ataaggagga tgaacgataa aatgataaaa atagatttga tcaccggatt
       61 tcttggtgca ggaaagacaa cctttataag aaaatatgca aaatatctga tggacagtgg
      121 aaaaaatatt ggtattctgg aaaatgattt tggtgcagtc aatgtagata tgatgatgct
      181 tcaggatctg atgggcgatc agtgcgagct ggaaatgatc tctggtggat gtgatccgga
      241 aacacatcgc agaagattca aaacaaaact gatctccatg ggaatgtgtg gatatgaccg
      301 gattctggtt gagccgtccg gcatttttga tgtggatgaa ttttttgata ttctgcacga
      361 agagcctctg gatcgctggt atgaaccggg cagcgtcatt acgatcgtgg atgcaaatct
      421 ggaagaagag atgtcggaag aagaaaatta tattcttgcg agcgaagcag ccagtgcagg
      481 caagattgtt ttcagcaaaa taggtaaaga tctgaaaaca gaaaaagagg cacaaatgga
      541 agaaaaagaa tctattgcaa aggcagaaga atcgatttcc aaagtgcttg cacatatcaa
      601 cattgcatta aaacaaatca aatgtgagcg tgtgatagag gaaaaagatt gtctgtgtaa
      661 aaattgggat acgctgacaa aagcagattt ccaggatctg atgtcagccg gctatacacc
      721 ggaaaattat cagaagctgg attttgagca ggattccgtt tttgaatccc tgtacttttt
      781 gaataaaaaa atcagcgtgg aaaatatgaa aaaagcggcg gaagagcttt ttcaaaatcc
      841 ggactgcggg catattttca gaatcaaagg attcttaaat accaactaca aagaagaagt
      901 aatgatggaa aataaaacga cagatgaaat ggaaaaagca gatttctggg aagaatttaa
      961 tgcaacacgc aaaaatatca gcatccagcc ggtaaaagaa ggacaggaag tgctgattat
     1021 gatcggggca gggctgaaac aggagaagat agaaaaatgc ctggagaggc attgcacaga
     1081 agggtttgag aaataaataa gaagttgtcg gtggtttcat ggcaggcgta aagaattgaa
     1141 ataccgagtg ttttgttaag atgctgtcaa aagctcctga cctgccagag gttgtttaag
     1201 cgggaattgt ttttcagacg caatgttttc tacttatctc taatccattg tttttacatt
     1261 atattttcaa aatgtaaata taatgtaaaa gtgaaatcag aggtattgtt ttttgataaa
     1321 ttgtggagta taaagaaatt acaaatacct caggctgcat gaacgtaagt ggtgcctgat
     1381 atgcgattct gaacagggaa agggtggttt tatgaagaag aaatttttga aattgttcac
     1441 gtcggttgca atgctggctc tcgtattctg tctccatcag aatgtccggg cagaggatat
     1501 tgcacagcca acggaaaagg atgtctacat ccatcacgat gatggagagg actatgtagc
     1561 caatcgctat gaaagagcga tcgtggtaga ccgtgtggta tatcagtatc ttccggagaa
     1621 agacagttac cggatcgtag catttgatga taatgacgaa gaatttccgg aaggcattac
     1681 ttttaagcct cgttcggaag taagaggcaa accggtcaca ggaatttaca ttgatggaga
     1741 agaggacgga ccgagctatc ttacacggct caatttagta ctcccggatt ctgtaaaaga
     1801 tattgagatc agcggtgcaa gttttggttc tatcacatta ccaaagttcc tgactgtcac
     1861 accgggagga atattcgaaa gtgattttga gcagattatt attccggaag gaactacaaa
     1921 tgtgagaggg atcaatgata tttggaaact ccgaaagatg gagcttccgt cttcaacaaa
     1981 gaaaatcgga aaatattttt tgagaaacag ttccgatctg agaaccgtat acattccgga
     2041 aggagttacc gagattggtg cagaagcatt ttccggatgt ccgaaacttg aaatttatat
     2101 cccggcaagt gtaaagaaga tcggtaaaaa tgcatttaag aaaacggatc agtacggaca
     2161 ggtaaaaatg atctattgtg ccaacaattc tgccgcacat aagtatgcga agaaaaatca
     2221 tcttccatac acaatcgtag atccggtgaa aacttcttac aaagcgacag acctgtcact
     2281 gagcacaaag cggatcagta tgccgatcgg tgcaaagaag agagtgttct atcaggtaac
     2341 gccggtttat gctgccaaac gtaatgtaaa attcagcagc agcaattcca aagtggtatc
     2401 tgtaaatgca aagggtatga tgacggcaaa gaaaaacgga aaagcaacga tcaccgttca
     2461 gttaaagagc ggaagcaaaa agaaagtgaa actgaaagtc gttgtccagc ctcgggctcc
     2521 gtatctttcg gcagacagcg ttgagaataa aaatacaacg gtatttacct ggaataaatc
     2581 cgaaggcgaa gaagggtatg agctcagctg ttccgatacg aaaaacggaa cctacaaagt
     2641 catcaaaaaa gtgaccggca aaacaaagat cacattcaag gcatcaacaa agaaatatta
     2701 caaaatccgt gcctggtata gaacagcaaa aggaaagaag tattacagtg attattcaga
     2761 ggccgtatat cacctgggac atatgtggtt ctgagattaa gattctgaaa aaatagatat
     2821 tttatgatgc cagggtcaaa aggtctaaat ccacatgagc ttgctcatag gtggatgaaa
     2881 gaccttggga cccgccccga tatgagcata aaagtgggat gataatccca cgatatgcga
     2941 atactggggt gctatgtgcc agtggcacat gtttagcacc gaccgaagcg gagcgtagaa
     3001 aggattgtgg gagtgcaacg cacggaacaa tccgtaggca tcatagttgg agacttttga
     3061 cacccatatc attttattga aaggctgcaa tcgtaaaagg ttgcagcctt ttgcacttga
     3121 cagaaatacg tggagcattt atactattgt taatacaaat aaaaaatatt aataataaat
     3181 atgtaaaaat ttcaaattat aaataaaatg acaaaaaaga acaggaaaag atatgacact
     3241 taaccttggt tcgctttgta tggcgatttt atggaacagt atttttattg gaattgtttt
     3301 ctggatagga aaaaagaaaa tttacacgac gcgaaaaggg attcatctga cattgatgtt
     3361 ttatttactt ggggttgtca ggatgatatt tcccattgat tttcgaccat ctatagggat
     3421 tcaagtgtgg aaaggggtat ttcctacagt taattatgct ttaagtattc aaaattacaa
     3481 tttatgggga aggcagttta caatagcaga tgctctggta gcaatctggc tgattattgc
     3541 aggggttttc atgttgcgtt attttgtgaa ataccaggaa atgcgtcgca tttttcaaaa
     3601 gtattccaca gaggcaaatg agcagtacca atcagttctt agcagaatca cacaggaaaa
     3661 aaagattaaa aagaaaatag cggtatatca gaatccgggg attactattc cttgtgcgga
     3721 aggtgtattc cggtggaata tttatcttcc ggactacgat tattcggaag aagaattata
     3781 ttatgctttg ctccatgaat gtacacattt cacaaatgga gatctgtaca taaaatggct
     3841 gacgcagctc tgtcgttgtc tttgctggtg gaatccactg gtttacaaaa tgcaggatac
     3901 actggaaaaa aatctggaaa tccggtgtga tcttactgtg accgaaaacc tcagtcagaa
     3961 agagcagttg agctatcttg gaacgatagt taagaccttg aaaaatgcac agaaaaaaag
     4021 aggcagcggt gttttatacg gagtccatct ggcagataaa aatggagaag agttaaaaga
     4081 acgatttgag attatctgca aaaatcagga taaaaaaaca ggacgaaatc aaaagagaaa
     4141 atatatggta tattttttcc ttatgattgt tctttcatat acggtggtta tacagccagc
     4201 ttatgatccg ccggaagatg agattataac agaagaaggt atggaagaac ttactccgga
     4261 taatacctat gttcaaaaag agggagataa atattatatg atatataaaa acgataaaga
     4321 tgagatatcg aaggaagatg cagaagaaat gaaaatgagt ggatttgagg tgaaagaaaa
     4381 tgaagagaaa taaggtggaa agtatattgt tgattgcagt gctggctctc agtatacaga
     4441 cagggagtta tagtgcagca ataaccgata gagagctgga tcaatattat gcaaagaaca
     4501 ttcaagagaa aagcacagat gttattgtat ggaagtatcg ggtatggaat ggaaagaagc
     4561 agaaaaggag gtggaatgta acaaaaagta gatgggaaga tccagcatgg aaagacgtat
     4621 gacggacagc gtgtagcaaa cagaagagaa aaggagaaga actatggcaa ataaaaaggg
     4681 agatacaaca aggattcagg cagttccaga tgtaaataaa tgtacaaatc tgggatgtta
     4741 ttctatgaaa gaaagggaag catatgcagt aaagacacgc aaagatcaaa tgctcagcac
     4801 catatattac tttccggatt ttgaaaaacc gtatactaaa acatttaagg tagagaaggg
     4861 ggcactggga cacggaaatg gtatgtgttg tgcagagaaa tatatatttt ttggctgctg
     4921 ggtaaaagat ccagataaaa gtggaagaga gcagcagaat tatttgattc gtgtcaaata
     4981 tggaaaattg acggataatc tgacaaatgg aattaacatt agcgttccag aagccacagg
     5041 agctattgct ttttacaaaa aaagccattt gattatcaaa gtgaaatctc gaaatggata
     5101 tttgactttt atgatgggta agatattaat caatgaaaaa ggaacggctg gaagatttga
     5161 tactctttgg acattttatg taaagaacac aacgggtttt tcaaggggac aggatatcta
     5221 ttacgatgca cagacagatt gtttgtttat accgcaatgt aataatgcta ataatgtaaa
     5281 taaaattatg gtagtaaatc tgtccggcga atcagaaaca tataaaggaa ataaactgta
     5341 tactccacag gatattatta cggttgataa gcagaaagac tatagcaaat atgaaataga
     5401 gtcattagta atagcaccga agagacatat tgttatggtg gcaaatattg ttccaaaagg
     5461 tactaaaaca gcagatgatg cagtggaaag gatatcaaat ttaaaatttt aaaggaggaa
     5521 cttttatgtt cagacaactc aaaaatatca tgttaaaggg cataatgttg tttgctttgt
     5581 tttgtatact tccggtaatg gttacaaatg cggcggataa tgaagcaaat ccgtttgaag
     5641 gaaaaattta tagctcgggt tatgcgtatt ttgaaaatgc agtggcggta aatcatgtag
     5701 tctatcggta tatgccagag acagacagtg ttcttgcaga atgtattgat ctgaacagta
     5761 ctttgaaaaa acatatgacg ctcaccatag aaaaaagtgt tctcgggaaa ccggttacga
     5821 ggattggtgg atattttacg gatccttatt atatggatgc aaaaatagga tatctttttg
     5881 ataaaattta tattccggat tcggtaaaaa ttattaccgg ttcggctttt agcgagtgca
     5941 caaatgtaaa aagtatctat attccggaca gtgtagaaaa aataggagat aaagcatttg
     6001 aagagtgtac gaacttgcag agtgttcgct taccgaatga tctggacaag atagagtttg
     6061 gatgtttcta taattgcaaa aatctgcgaa aaattgtca
//
LOCUS       DAWDWH010000122         6084 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5639_length_6084_cov_8.413170, whole genome shotgun sequence.
ACCESSION   DAWDWH010000122 DAWDWH010000000
VERSION     DAWDWH010000122.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5639_length_6084_cov_8.413170"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1983..1992
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4342..4351
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5425..5434
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5691..5778
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1504 a         1475 c         1060 g         1927 t
ORIGIN      
        1 agttcagtac acggtttctt cctacattca tcaatgcaaa gatcataccg atgatcatgg
       61 caaaaagcaa tgacagcagt gtaagaagca aagtctttcc gagtgctcca agaagcatgg
      121 agctgtaaga ctgtaaaatt gcaatcatgt tctttctcct tcctttgtat ttttataaga
      181 ttgagattca aaatacctta aaacactgct gtgcattttc cggcttttga ttctgctgtt
      241 atttattgta tcataacatc tattgcctgg tcaaaaggta ttttgtgata gcgtaagaag
      301 cacggaatcc aaaaccggtc ccgtgcttcc atgcataggg tatcctctaa gagttgccct
      361 ttttattctt ctgattattc gcccagatat tttgcgatga tctcatcgta agtaccgttt
      421 gctttgatgt cttccagtcc tttgttgaac atatccagca gttcctggtt tttctcgtcc
      481 atgattgcca gtccgtaagg tgcaccgtcg ctctccatac cttccggaat cttcagtgcc
      541 agattacctt ctttgataga agctgccatg atcggcgtgt cttctacgca tgctgcactg
      601 ttgccaagga taacatcctg atacatcgtc ggtgaatctt caaataccgt aacggtaaat
      661 ccatactcat ctttcaggct gtttgcaaag tcagcacctg cagtaccgtt ctttactgcc
      721 acatttttgc ctttcagatc ttcgtagctt gcgatgtcgc tgtcttcaga tactacgaaa
      781 gtctgtgttg cgttataata tccatcagaa aagatccatc cggaatcgat acgttcctgt
      841 ttgatcgttg ctcctgcgat cagagcatct gcctgtcctg cctgaacagc tgcaacacct
      901 gcatcccatc caaggctctg cagttcatac tcaaatccct gatcttctgc gatcgctgcc
      961 agaaggtcta agcagttgtc tctgctgttt ttgagccaca tcctgccagc atacctgctg
     1021 tcattacagt acaaagtaag aatgccattg cttttttcat ttctaattcc tccatatgat
     1081 caaaatgttt ttagaatagc aagaagcgaa aagccgattg cctgtatccc aaagccccct
     1141 tgccttccgc tacattgcat atcatataaa aaatgtttgc agctgtcaag aaaaaagtat
     1201 aaactttgtt acagttttgt caatttctca caaaaatatc tttgaccaga ctctgtttct
     1261 atgctatact aaacttactt ttcttattgc gataggaaag aacgtgaggt ggagcaactt
     1321 atgatagtaa atgaatcagc agaaaattac ctggaaacca tattagtgct cagcaagcag
     1381 cttccggtgg tccgttccgt ggatgttgcc aatgagcttg gatttaaaaa atcaagtgtc
     1441 agcgtcgcta tgaaaaatct ccgtgagaaa aatcatatca cagtgacaaa ggccggattt
     1501 atttatctga ccgattccgg cagagcaatc gccgaaatga tctacgaacg ccatcagctc
     1561 atctcctctt gtctgatgaa actgggagtt tccgaaaaaa ctgccgtgga agatgcctgc
     1621 cgcatcgagc acgtaatcag caaagagagc tttgacgctt tgaaaaacca cataaaaaaa
     1681 atgcaaaaag aacagtgaag aaatgagaca aacccgattt tggatttgtc tctttctttt
     1741 tataccgttc tcttctgtct gccttttctt acttctcttt cagtgcacgc atggagttca
     1801 ggatcgcaat gacagaaact ccgacatctg cgaaaacagc ttcccacata ttggctgcac
     1861 caaaagctcc gagcagcaat acgattgctt ttacgccgag tgcaaaaacg atgttctgtt
     1921 ttacgatcaa cagtgttttt ctggaaatcc ttacgatcga tgcgatcttg cggacatcat
     1981 cannnnnnnn nngagcatca ttgatgccgt ctccgacaaa tgccagtttt tctttcttcg
     2041 gtttttccct caggaggcgt tctacctgtg ccaccttatc tgccggcagc agttctgcat
     2101 gtacttcatc aataccaagc tcttttgcca cttccatagc cgcctctttg cggtcaccgg
     2161 tcagcatgac gcattttttg acaccaacct gtttcatagc acttatggct tcttttgcac
     2221 cgtcttttac tgtatcagaa atcaccagag ttccggcaaa ttttccgtca catgccacat
     2281 agacaacggt tccgccgctc tggcaagggg tgtaagcgat gccttccttt ttcatcagtt
     2341 tttcgtttcc gatcagcatt gctttattgt caaccgtgat ggaaatacca tgtccggcga
     2401 tctcatttgc attttctgta cgcttcatat ccggagtttt gccgtaagct tcccggatcg
     2461 aatttgcgat cggatgtgta gagtaacctt ctcccaaagc cgcaatctcc agaagttctg
     2521 tctctgtcat gccctgcggc tggatctgtg caaccttaaa ttcgcctttt gtaagtgttc
     2581 cggtcttatc aaatacgatc gttgtcatct cagcaacggc ttccagatag ttgcttcctt
     2641 ttaccagcac accgactttg gatgctgctc cgattccgcc gaagaatccc atcggaaccg
     2701 agatgaccag tgcacatggg caggagataa ccagaaaaat acatgctctc tggatccatt
     2761 ctgcaaatcc gcccccaaga accagtggag gaaccagtgc cagaaccaca gctccgatcg
     2821 tgacaaccgg agtataatat tttgcaaatt ttgtgataaa gttctctact tttgctttct
     2881 tgctgcttgc attctctacc agctccagga tcttcgcaac cgtagaatca tcaaattctt
     2941 tcgtaacttt tactttcagt gtaccgcttc cgttcacaca tccgctgatg atctcatcac
     3001 cggcctttgc acttctcgga accgactcgc cggtcaatgc tgcggtatca acaagcgatt
     3061 ctccttcgat caccacgccg tccagaggga tacgctcgcc cggtttgatt acgatgatat
     3121 cgccaacctc cacgtcatcc ggatcgacct gtgtcagcac gccgttctgc tcaatgttgg
     3181 catattccgg acagatatcc atcatcgcgg agatggactg tctggatttg ccgaccgcat
     3241 agctctggaa cagttcaccg acctgataga acaacataac ggctactgct tctgaaaact
     3301 cctgtacgcc aaaggctccg acggttgcaa ccatcatcag aaagttttca tcgaaaacct
     3361 gtccgtggct gatatttcga actgctttgt acacgatatc atacccgatg atcagatacg
     3421 gtaccagata aaccacaaac tgaatccaca cattcacgtt ctccaacatt cctgtgtgct
     3481 ctgcgatcag cagggcaaca aacaggacaa atgcagcaat aatgcggccc agcatttttt
     3541 tctgtttttc cgtcatacca aaatcctccg tttttgaaag cagcagtcgg atatcgctat
     3601 ccaccctgct gccccctttt ctttcacctt attttcatat tgggatcaaa attgatgcct
     3661 gcgaattgct ccgttacaaa gtaactctca caatgctatt tcatcaggat cttgcagtcc
     3721 ggctctactt ttgcacatac acgtacgact tcttccatga tcgcatcgaa tttgtcatcc
     3781 tctgcatcaa tggtcatttt ctgcatcata aagctgacat ttgcatcgtt gactcccggg
     3841 atttttttga tcgcatcttc catttttgct gcacagtttg cacaatccag atcctgtaat
     3901 ttaaattttt tcttcatgat aaaacactcc ttttataata tttcttcttt atttttcttc
     3961 aatatgttcg cggccctgtg cgatgatcgt acgcacatgc ccgtctgcca gagaataaaa
     4021 aaccgacttt ccttcccttc tgcttttgac cagcttggat tgctttaaaa tccgaagctg
     4081 atgtgagatc gccgactgtg tcatatgaag cgtctctgcg agatcacaga cacaaacttc
     4141 tgcttcaaac agcacaaaca gaatacggat tctcgtcgaa tctccaaata ccttaaataa
     4201 ttccgcaaga tcgtataatt cctcttcctc cggcataccg ctgttcacaa tatccagccg
     4261 atccggatga atctggcagt cctcacagca ttccagttct tccgttgcca agtttccacc
     4321 ctctttctgc aaacatttga annnnnnnnn nagctcatgt ggatttagac cttttgaccc
     4381 ctggcatcat tgtattatcg ttttgcaatc accttcactt tgctgtatgt tccatatttt
     4441 ttagctccat ttatcttctg atatgcccgc acacgcacat aatatttccg gttctttttc
     4501 agttttttga ggacaacact tgtttttccg gtgctccgtt tcttcgcctt gcggaatttt
     4561 ttattatccg catagctgat ctgataacca tctgcctttt ttactttctt gaacgtgatt
     4621 ttcgcttttg ttgtaccggt tcgtactgcc tgaagttttt tcggtgctac cggttttttg
     4681 acttttggcg gatttggctt tgaatcatgg tctgagttct gattcgaatc tggtgcagat
     4741 gccttatagt aaataacaat ctcatccttg cccggatgtc cgctcagatt ttcgatttgc
     4801 agcttgtttt ttgcattttc ctgattgtat gtgtaagtta catcaccgat tttaaaactt
     4861 tcttccggct gatgcaaata agactcgcca acaatcagtc catctttatc ttcctgacgt
     4921 accagtttcc cagtagcgga atccatataa gaaacacgaa ccgccgcaga atctcccgga
     4981 tttttctttg taaagtagta tgctttaatg acattgcctg ctttatagac atcactgcct
     5041 ttttcgattt tcttggccgt gatagataaa tgattatttg cataatcttt atcaaaatag
     5101 taaacatttc cattgctggc agtcagttta ttcggtacag ctgtgtagtt aatttttgcc
     5161 cccggacttg taaatcgttg atctgtttta aatgctttgc cagtatccgc atctacaaac
     5221 tccaatacta cctttgtact ttcttctgca taatccccag gacgcaattt atacaaatga
     5281 tggtatgtct ttaagatatt atcattcaga ttcgatgaaa gtttttcgat atatattttt
     5341 tcattcagtt tccaatcata actataataa cattcatata atcgccttaa ttcgctcaaa
     5401 tcattatcat tatcctgtcc acttnnnnnn nnnnacttac tttaatggac aaatgattat
     5461 ttttattgtc cttatcaaaa tagtaaacat ttccattgtc agtagtgagc ttatttggtg
     5521 caggataagt tctcgttgat tcaataaaaa catatctcct atctgttcga aacgcctttc
     5581 cagtatccgc atcaatcaat tcaataacaa cctgttcttt attcatatgt ccgcctaaaa
     5641 tatcccatcc atgatttatt gttcttacga tattttttgt gagatctgcg nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnac attctctttc ggattttcag aaaggctttc tattgtcagc
     5821 acatttttcg gagattcgaa atcaaacata tatatttctc cattctcccc ttctatactt
     5881 tcacttactg cctcttcttt acaatatcca tattcccctt tttgtgtctg actgaccgtg
     5941 taaggtcttc ctgtcattct gtctacatta cgatgttcta ctttaaaaat ttccccggta
     6001 tcttcatcga tgcattgata agaaacctaa tcttattagc tgccagtgct gaagcaggat
     6061 gcagatacaa aaccaccagt aaaa
//
LOCUS       DAWDWH010000123         6054 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5664_length_6054_cov_8.588931, whole genome shotgun sequence.
ACCESSION   DAWDWH010000123 DAWDWH010000000
VERSION     DAWDWH010000123.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5664_length_6054_cov_8.588931"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4787..4796
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1893 a         1201 c         1553 g         1397 t
ORIGIN      
        1 gaacgttatc tggaactggt tcgaaaactt cgggcggcac ttccggatat ttctctgaca
       61 acggatatta ttgtcggctt tccgggtgaa acagaagaag attttatgga gacgatggaa
      121 gtggtaaaag aagtgcagta tgacagtgcg tttaccttta tctattccaa gcgtaccgga
      181 accccggcag ctgttatgga aaatcagatc ccggatgatg tagtgaaaga ccgttttgac
      241 cgtctgcttg cagaagtaca ggaaatttcg agagaacgct gtgcaagatt tgaaggacag
      301 acgatggatg ttctggtgga agatgtcaat gaccatgatc cgtcccttgt caccggaaga
      361 ctgactaaca acctgctggt acattttaag ggtacagaag acttgatcgg aaaaatcgtc
      421 gatgtgcatc tggatgagtg ccgcggattt tattatatgg gaacgatgat ttcaaaataa
      481 agccaaaaga aacaaatcga agaaaacagg cgtattttgc tttttcagtc agaaaaggcg
      541 gggtacgtct gtattcatga acataaggag aggtttatgc agcaggagac accaaagccg
      601 cacaaacggc gtgtaagata taaaggaaaa tatccgaaaa aatttgaaga aaagtacaaa
      661 gagctgaatc cggaaaaata tcaggatacg gttgaacatg tgatccgaaa agggaataca
      721 ccggcaggta tgcacatttc cattatggtg aaggaaatcc tggattttct gaaaatccag
      781 ccgggagaaa ccggatttga tgccacgctt ggatacggcg gccatacaaa ggcaatgatg
      841 gaatgtcttc atggagaagg ccatatgtat gctacggatg tggatccgga agagtcggca
      901 aagacaaaaa agcgtctgga agaacaggga tttggggaaa ggattctgac cataaagctt
      961 cagaatttct gtacgatcga tgagattgca aaagaagcgg aaggatttga ttttattttg
     1021 gcggatcttg gtgtatcttc catgcagatc gacaatccga aacggggatt ttctttccgt
     1081 gcggacggac ctttggatct tagactgaat cagcaaaaag gaatcagtgc ggcagaaaga
     1141 ctggatcaga tcagcagaga ggaacttgcc gggatgttat atgaaaattc agatgaaccg
     1201 tactgcgaag agatcgcaaa agcgatcaca gatgagatcc gcaaagggaa tcgcatcgat
     1261 agcacaacaa aactgcggac tgttatcgaa aatacgctgg attttcttcc ggaaaaagag
     1321 aaaaaagata cgatccgaaa gacctgccag cgtgtgttcc aggcacttcg cattgatgtc
     1381 aaccaggaat ttgaagttct gtatgaattt atggaaaaac tgccggatgc cttaaagccg
     1441 ggcggacgtg tggcaatcct gacgttccat tccggggagg acaaactggt caaaaaagca
     1501 ctgaaggccg gatacaaagc aggtatttat tcggactatg ccaaagatgt gatacgtccg
     1561 tccatgcagg agtgtgcaca gaatggcaga gccaggtcaa cgaaaatgcg ttgggcggta
     1621 aaagcagagt aagaacagag gtacaagcat gttttgtgca gaacaggcac aaacaataga
     1681 aatgagatga aagaggtttg accgttgagt acaacagcaa aaaataataa aaaaaattcc
     1741 gtaacgccga tgatggcaaa atatctggag acaaaggaac agtacaagga ctgtattctt
     1801 ttttatcgcc tgggtgattt ctatgaaatg ttttttgacg atgccctgac ggcatccaaa
     1861 gcactggaga tcacactgac aggaaaagac tgcggacttg aagagcgtgc tccgatgtgc
     1921 ggcattcctt atcatgctgc agatacatat ctgaaccgtc tggtggcaaa aggatataag
     1981 gttgcaatct gtgaacaggt agaagatcca aagcaggcga agggactggt taagcgggaa
     2041 gttatccgca ttgtcacgcc gggaacgaat ctggatacta acgccatgga tgagacaaag
     2101 cacaattatc tgatggcgat tgtgtgcatg gcggatcgtt ttggcgtctc cattgccgat
     2161 atttctaccg gtgattatct ggtgacggaa gtggatggat gcagaaaact attagatgag
     2221 atcacaaagt ttgcaccgac cgagatcatc tgcaacgatt ccttttttgt cagcggtgtg
     2281 gatgtagaag atctgaaaaa ccgtcttgga attgccgtca atgccctgga aaactggtat
     2341 tttggtgatg aacgctgtaa gaagacactg ctggaccact ttcatgtctc cagcctggca
     2401 ggactcggac ttggtgagct gaactgcggg atcatcgcag ccggttcact gcttcagtat
     2461 ctgatggaaa cacagaaaac agcgatcgca aacctgactt cgttaaatac ttattttgcc
     2521 ggaaaataca tggtgatcga cagatcgacc agaagaaatc tggagctttg cgaaacttta
     2581 cgtgaaaagc agaaaagagg ttcccttctg tgggttctcg ataagacaaa gactgcgatg
     2641 ggggcaagac ttctgcgttc ctttattgag cagccgctga tcgacaagga agaaatatca
     2701 aacagactgg aagccgtcgg agaattaaaa gacaatgcga tctgcaggga agaactgcga
     2761 gaatatcttt ctccggtcta tgacctcgag cgtctgatca gtaagatcag ctacaagact
     2821 gcaaatccaa gagatctgat cgcattcggc acatctctgt ccatgctgcc gcatatcaaa
     2881 tacattttat ctacgatgaa atctccgctt cttgtggaag tatgtgagca gatggatgaa
     2941 cttcaggatg tgtgtgatct gatcgaccgg gcaattatgg aggagccgcc gatccagatt
     3001 aaagacggcg gtatcatcaa agaaggctat aacgaagaaa tcgaccgtct gcgtgccgca
     3061 aagacagaag gcaagacctg gctggcacag ctggaagcca aagaacgtga aaataccggc
     3121 atcaaaaacc tgaagattaa atacaacaag gtgtttggct attatctgga agtgacaaac
     3181 tcttttaaag atatggttcc ggattactat acaagaaaac agacactcac aaatgctgaa
     3241 cgttatatca cgccggaatt aaaagagctg gaggacatca ttctcggagc ggaggacaag
     3301 ctcttttctt tggaatacaa tctgttttcc gatgtaaggg aacagatcgc aaaagaggta
     3361 gaacgcattc agaagaccgc aaaagcagta gcggcgatcg atgtcttcgc ttctttggca
     3421 ctggttgcag aacgtaataa ctatgtctgc ccgaaaatga atgaaaaagg cgtgatcgat
     3481 atcaagaacg gccgtcatcc ggtagttgaa cagatgatcg aaaacgatat gttcattgca
     3541 aacgatactt accttgacaa cagcaaaaat cgtatttcca tcattaccgg tccaaacatg
     3601 gcaggtaaat caacatacat gcgtcagagt gccctgattg ttttgatggc tcagatcggt
     3661 agctttgttc cggcatcttc tgcgaagatc ggcattgtgg accgtatttt tacaagggtc
     3721 ggagcttccg atgacctggc aagcggacaa agcactttta tggtggaaat gacagaggtt
     3781 gccaatattc ttcgaaatgc aacttcgaac agtcttctga ttctggatga gatcggacgc
     3841 ggaacgagca cttttgacgg gctttcgatt gcatgggcgg tggttgagca tatcgcaaat
     3901 acaagacttc tcggggcaaa aacgcttttc gcaacgcatt accatgaact gaccgaattg
     3961 cagggcaaat tatcgggtgt gaataattac tgcattgctg taaaagaaaa gggcgatgat
     4021 attgtatttc ttcgaaagat tgtaaaaggc ggtgcggata agagttatgg tatccaggtc
     4081 gcaaaacttg ccggtgttcc ggattccgtg atcgaacgtg ccaaagagtt ggtagaagaa
     4141 ttaagtgatg cggatatcac agagcgtgca aaagagattg aggcagcagg cagtgcaccg
     4201 caggcaaaaa cagcgacaaa agtccgcaac cgtcccaaaa cctacgatca ggttgatatg
     4261 gggcagatgg cactgtttga cactgcaaaa gatgaggata ttttaaaaga attaagtgag
     4321 atggagattg cgtccatgac accgctggaa gcattaaata cattgaaccg tctgcagagc
     4381 aaactgaaaa accgttggag tgtaacaggc gtacagggat gatagtatga agaatattgc
     4441 agtattagat gaaagtacca ttgataaaat tgcagcagga gaggtaatcg agcgaccttc
     4501 ttctgtggtc aaagagctgg tagaaaatgc catcgatgcc ggagcgactg ctgtgacggc
     4561 tgaaatcaaa gacggcggga tctcattgat ccggatcacc gataacggcg agggcattgc
     4621 aaaggatcag atttcaaccg cttttctgcg tcatgcgacc agcaagatca cgaaagtgga
     4681 ggatctgctt tctgtatctt cgcttggatt tcggggtgag gcgttatcga gcattgccgc
     4741 agtctgccag gtggaagtga tcaccaagac ggccggcaca ctgctcnnnn nnnnnntttc
     4801 tggtgcgcaa tcttttttat aatacaccgg caagaaaaaa gtttttaaaa tctgcgatga
     4861 cagaggctgg atacatcagc gaactgatgg aacgcatggc tctttcgcat ccggaaatcg
     4921 cgttcaaatt tattaataac cgacagacca ggctgcacac atcaggcaat cataacctga
     4981 aggatattat ttatggtatt tatggaaggg atattgcggc caatctgctt ccggtaaaag
     5041 cagaaggagc atttggcagg ctggagggat ttgtcggaaa gccggtgatc tccagaggca
     5101 accgcaattt tgaaaactat tttgttaatg gaagatatgt aaaaagcaag atcatcacaa
     5161 aggcgatcga ggatgcctat aaaacattta tgatgcagca taaatatccg tttacggcac
     5221 tgcactttac gataccgggg gaccttctgg atgtcaacgt tcatccgacc aaactggaac
     5281 tgcgtttttc caatcaggaa gaaatctacc agatcgtata tgatactgta cggcaggcac
     5341 tctcatccag agaactgatc ccggatgttg gtgtggagag ggagccgaaa aaggcagaac
     5401 tgccaaaata taagaagccg gcacaggttc cggagccatt tcagctccca aaggcaaaac
     5461 cacagtcctc ttttacgaca catgtgatac gggagagtgc accttcgtac ggaaatctgc
     5521 atacagccac atctgatccg gctgttgtac atgcttccaa gcagatggca gtttctatgc
     5581 agaatccagt ttctacgcag gatcagacaa aggcagagct gccaaaaaca gaaaaagctc
     5641 cacgtccgct gccgcagatg ttccagaaac aaaatacaga gccggcatca aagagcgtac
     5701 cagaaaccga aaaaggagaa gtgtctgaaa cagcagaaaa accagagaaa ccgttcgaac
     5761 aggaaatcac cagagagatt cagaaagcag aacagatgga gctttttgat gataagcttt
     5821 tatcgcagca gaacgtgaag cagcatacgg tcatcggaca agtgttcgat acttactgga
     5881 tcgtgcagtt tcaggataag atgtatctga tcgaccagca tgcggctcat gaaaaggttt
     5941 tatatgagca gacaatgaaa cgattaaaag atcatacgtt tacgtcgcag cggatcagtc
     6001 cgccggtcat tctgacgctg agtctcaaag aggaggaagc tttaaaaatg caca
//
LOCUS       DAWDWH010000124         6029 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5684_length_6029_cov_14.305490, whole genome shotgun sequence.
ACCESSION   DAWDWH010000124 DAWDWH010000000
VERSION     DAWDWH010000124.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5684_length_6029_cov_14.305490"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5396..5405
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1812 a         1146 c         1687 g         1374 t
ORIGIN      
        1 ctatcattcg ccgaaaatct cctgtgcaaa gccattttct ttcccggtcg ggacacagga
       61 tattctggat agtgtctatg cgacagactg catcgacgga gatatctcaa ataaggtgga
      121 gatcacgggg gattcggtgt ttttcctgaa tatcgccgga gaatatgaga tctggctgca
      181 ggtgacaaac agctgcggtg atatggtgac ggttccggtc acgcttgaga tggtggatta
      241 cagacagcag acggagagga caaagcgtgc agaagcagca aaacagacgg aaaggaccaa
      301 tctaacagaa aaagcgacag aagaaacagg gcagaaagag acggaaggag cagaaaatgg
      361 aacaaaggcg gggtgagtac aggatggatg gagtggacag ggcactgtcg tatacggttc
      421 tgacggatgt tctgcggcag tggctggtga ttttctgttt tgccgtttcg gcagcattac
      481 ttgcggcagc tgtgatgcag tggcactatc agccatctta cacttcccag gcgacacttt
      541 atgtgacaaa aaagggactt ccgaataatg tctatgacaa tgaaagttcg gcacaggtta
      601 tggcggaaca ttttgagcag attttaaaca gcagtatttt gcagaaaaaa gtcgcagaag
      661 agatacatct tgcggatttt gcaggcagtg catcggcaga ggcggtggag ggaacgaatc
      721 tgatcaatct ggcggtcact gcggcctcac cggagctttg ctttaaactg atgcagtcta
      781 ttttgaaaaa ttacagcctg gtatcggact atatgacagg taatgcaatc ctggagatct
      841 taaaggcacc gaaggttccg cttacgccgg atcagccgtt tgagcggcga aatatcatga
      901 tcaaagcatt tctggctgcg gcagcatgct gggtactgat cttcggcggg ctgtcctggc
      961 tgaaggatac gatccgcacg gaggaagaca ttgaacagaa gctggaggcg agacgtcttg
     1021 gtacgctctg gcatgagcgg aacagaaaaa aacggactga catccgcata gacgatccgc
     1081 tgatcagctt ccgctatatg gaatccctgc aaaaactgac acagcacgtc cagaaaaaga
     1141 tggaggagcg aaaggcacag acactgcttg tgaccagtgt gctggaaaaa gagggcagat
     1201 cgaccgtggc ggaaaatctg gcactggcac tttcgatgcg aaaagagaag gtttttctgt
     1261 taaatgcaga tttccggaag cagaatgcat cctggcttgc cgatgaagaa ggacatttga
     1321 aaatagaaaa ttttcagaaa ttgctggaga acctgaaaaa agagaatgat tatgtgatca
     1381 tcgatacgcc gccgtttttg cagaatgcgg atacggagga gatggcacag atggcggatg
     1441 cgtcacttct ggttgttgca gagcaccggg cacaggcgaa agatctgaat gcggcactgg
     1501 atcttttaaa tgcacaggga gagaaaaatc ttggctgtgt ctacaacaat gcacatgtgg
     1561 aatttttaag accgatggcc tcttatggct atcagtatgc atatcattac ggcagatatg
     1621 gaggacatta tgaacagtga gatgagattg gaggatggaa ggatcgacct tacctctctg
     1681 gcagagacga tgtggaaaca gggcagacgc ttctggtggc ttttgctgct tcttgcaggg
     1741 atcggggcag ctgcctgttc ttttcttgtg aaaaaaacgt atcagccggt ctatactgca
     1801 tttgctgctt atgcggtgag cagtgattct gtctatgcgg gaaataaaaa ctatgagcag
     1861 aaagtagcag aacagatcgg cgttgtgttt ccttatattt taacgagcga tgtactgcgg
     1921 gaaaaagtat gtgaaaagat ggggacaaag accatgccgg gaagtatcga tgcatcggtg
     1981 gttcaagata ccaatctgat cacgatccgg gcaagggctg caagtgccgg cagggcgtat
     2041 gaacttctgc aggcagtgct ggcatgttac ccggaaatcc tgcatccggt gaccggtgta
     2101 ttgaagatgg agcttctgga tgaatccggt gtgccgcatc agccggacaa cattccggat
     2161 ggtcgaaggg tagctcttga aggggctgcg ggtggaagcc tgctttttat tctgatcgtg
     2221 ctttttggtg ccatggcgaa aaagaccgtc agaaaccggg aagatctgca gagactgacg
     2281 cagatccatt atctgggaag tcttccgaaa accagggcaa aaaagagatc gcctgccttt
     2341 ttagatgccc ggtatgccat ctgtgcaaga ctggaaaagg agctgaaaaa gaaaaacatg
     2401 catacgcttc ttgtcaccag tgccgttgcc ggggaaggaa agaccaccgt tgcctgcaat
     2461 ctggcactgg ggctggaaaa gaaaggctat tcggtacttc tgctcgatgc ggacttaaga
     2521 aaaccggctg tttgccggac gattcagctg gattctggta cggaaggggc gtatgaagtc
     2581 ctgaccgggc agagaacggc agaggaggta aaaatccgct gtcgggatac acagctctgt
     2641 gtcattgcag gaacgaaaag cctgtcatcc acgcagcaga tccttggcac agggaaaatc
     2701 gcagagatca taaaacagct ggaaaaggag gcagatctgg tgatcgtgga cacaccgccg
     2761 gccgggattt tgtcggatgc ggcaatgctt gcttcctgcg tggacggagg tgtttttgtt
     2821 gtaagacagg attttgctga tgtaaggatc ctgacggaag gaataaggga actttccgaa
     2881 gccgggatgg agtttgcagg gtgcatttta aatcagaccg aacacaaata gcagtggaca
     2941 atgggaacgg tttcttatat aatgaaaagc atggaataag ataacagtta cttaagatag
     3001 atctaaggag cggatgaaat atccgctttc ttcgattctg caagaagaga ggaaagacat
     3061 gacaataaga gaatgttacg aaaagaccgg gtctgattac gaaagcattt tgaaaagatt
     3121 tggcagcgaa gcgatgatcc agagatttgc actgaagttt ttaaaggacg gcagtttttc
     3181 ggatctgaaa gatgccctgg aagcaaaaga tggagaaaga gctttccgtg cagctcacac
     3241 gctgaaaggc gtatgcatca acctgggatt tgaccggctt tataacgtaa gtgcagagct
     3301 gacagagaaa ctgcgaggca gagagatcga agagagcgaa gagctctttg aacaggttga
     3361 aaaagaatac catgccctga ctgctgtatt aaagcagtac gaatcagaac tttaaaaaga
     3421 tcggatgcga aagagaaaat tgcagtgagg agatgaactt atggaatcgg caaacggtat
     3481 aacaaggcag caggcagaag acgaaatgca gcttttcaag aaagtcttta ccgttgtacg
     3541 gctgctggat ggagaacatc tggaacagat gcataagaga tgcaagctgg actgtaaaga
     3601 cgagaaagag aaaagtgagt gttatgaaat atggaataaa gaccatccat gtgaaaactg
     3661 cacttccctg caggcactgt atcagcggac acagaagacc aagcttgaat ttatcgagga
     3721 tgaggtttat caggtcactt ccagatatat aaaagtagac ggaagagact gcgtgatgga
     3781 gcttgtcaaa tacatggaca tggatacgct ggtggattcc cgtggctgtg aaaagctggt
     3841 caatcagctg atcgagtatg atcagagatt gtataaagat gcactgaccg gtgtatataa
     3901 ccgtcgctat tttgaagagc agatcaagaa aaagaatgaa aatgccgggg tggctgtcat
     3961 tgatatggat gacttcaaac tgtgcaacga tacgctcggc cacagtgcgg gagatgccgc
     4021 actcagaacg atggtaaaga cagttaaaca ctgtatccgc aagacggata tcctgatccg
     4081 ttacggcgga gatgagttta ttctgattct gcagaacatc aaaaaagaaa attttttcaa
     4141 taaactggtc cagatccaga gtgatatcca tgctgcaaac gtaccgggat ttccaaagct
     4201 tcagctttcg gtcagtatcg gcggagtgat atcctcaaaa gagacggtcg agcatgcagt
     4261 tatgcaggca gataaactga tgtatcaggc aaaagagcat aaaaatatgg ttgttacgga
     4321 agataatgcc tcctatgttg caccgggagt ggcaaacaga aaggcacggc agcagattct
     4381 gatcgtggat gactcggaga tgaaccgtgc cattctttca gagatgctga aaaaggattt
     4441 tgatattctg gaggcagaaa atggaaaaga atgtctggat atcctgcagc agtacggcat
     4501 ggggatttcc ctggtgctgc ttgatatcgt aatgccggtt gtggatggat ttgaagtgct
     4561 ggatatgatg gtacataacc actggatcga ggacattccg gtcattatga tctccagtga
     4621 ggattcagaa ccttatatcc ggcgtgctta tgagcttggc gtatcagatt atatcaaccg
     4681 tccgtttgat gcaaaagtag tatgtcagag ggtgtataat acaatcaaac tgtatgcaaa
     4741 acagagaaaa ctggtgacgc tggttaccga tcagatctac gaaaaagaaa agaacaacca
     4801 gatgatgatc agtatcctaa gccagatcgt agaatttcga aacggtgaga gcggacttca
     4861 tgtattacat atcaatattt tatcagaact gcttctggag cgtctggtac agaagacgga
     4921 taaatatcag atatcggcac agaggcgttc tctgatcacg acggcttctg cactgcatga
     4981 catcggcaag atcggcatcg atgacaagat cctcaataaa ccgggcagac tgacaccgga
     5041 agagtttgat gagatgaaaa agcacacgat catcggagag tctatcctga aaaacgtcgg
     5101 tatttatcag aatgaagagc tggtgcagct ggccatgcat atctgcagat ggcaccatga
     5161 gcgttatgac ggcaggggct atccggatgg actgaagggc gaagagatcc cgatcgaagt
     5221 ccaggttgta tcgatcgcag atgtctatga tgcactggta agcgaacgtg tctacaaaaa
     5281 ggcatattcg cataaaaaag cagtggaaat gatcctgaac ggtgagtgcg gacagtttaa
     5341 tcctctgctg ctggagtgtc tgcaggatat tcaggaccgt atcgatgaag agtttnnnnn
     5401 nnnnnagcga agaagagaat caggagcgaa aggtacacga tcagatcatg gagacgacgg
     5461 tcttgggatg agaaaaagag ggcagtactg ttgctctgcc ctggcatgac gctgctgtgc
     5521 ggctgttcca aaaaagctgc tctgccttga cttttgatgc aaattttgat atgattgcgt
     5581 cagtagtcgc aatacatcag gaggaactgt ccaaggcaga tctggcaggt gatgaacgat
     5641 ttatacaaca aaacgaaaag gtgggatttt tgatgaaaca gtttacttac acaattcagg
     5701 attcaatggg tatccatgca agaccggcag gtctgcttgt aaaagctgca gcagcattca
     5761 aagacacaac gatcgagatc agcagcaacg gcaaaaaagc tgacgttaag agaatcatgg
     5821 caatcatgag catgggcgtt aagcagggtg cagaagtaac cgttacctgt gaaggtgcgg
     5881 atgaagatgc agcagcagaa gctatggaga aattcttcaa agaaaatctg taagatttac
     5941 tgccgtcagg gagactgaca gagtgatttt aaaaagtata atgaaagagg catggccgga
     6001 gacggttgtg cttctttttt agtgacagg
//
LOCUS       DAWDWH010000125         6013 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5696_length_6013_cov_13.832662, whole genome shotgun sequence.
ACCESSION   DAWDWH010000125 DAWDWH010000000
VERSION     DAWDWH010000125.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5696_length_6013_cov_13.832662"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5571..5580
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2054 a          931 c         1516 g         1502 t
ORIGIN      
        1 aaagtagtgg aagtaacggt ttcctttttt gttcttaaaa acaattctgt tacgaaaaca
       61 attccaaaaa atataaaaag gtgcagcatg aaaaaaataa tgattctgat aagcgtaatc
      121 tttttgatgt gtacaggtat atggtttcga tttcatacga aagagtgccg tgtagtatcg
      181 gatctggcat atgataaaaa taaaaagggt gatcatctgg tatatatccg ggaagggcat
      241 gaactggttc catttctggt attgacttct gattataatg gcgagacact actgttgagg
      301 aaagaagtac tgaaaatgaa tatgcagata aattcttatg aagcgtatta tgaaaacagt
      361 tttatggatg attatttaaa ccatgaatat atcagacagc ttccggatgg gatgcctgtt
      421 ttggaatctg agattaagat aacggatgag aaatcattgg gagtttcggg aacagggaca
      481 aaaagtataa agagaaagat ttttttgctg gcatacagtg aggtgataga agatcagaat
      541 ataaatgcag catcggaagg aacgtttctt agattttttg agaaggaaga acagcgtatt
      601 gttttgaaaa aaaatcaaaa aagcagctgg tggctgcgaa cgccgaacac atattatact
      661 tcctgcgttt atgttattgg ggcgaataac aagatcggct tttccaatgc atatgataaa
      721 aatggagtgc ggccggcatt ttgtgtgccg ggggactgca aaatggaaac cgatcaggat
      781 ggacacagag tattgcagca gaggcagtaa aggagtttgg gaatatgaga aaaatgaaaa
      841 ggatcatcag cctttggctg gctgttattt tagtgatcac cggtgtggat ctgccatttg
      901 gcatcctgga aatccaggcg gcagcaaatg tgaagcggta tacggtattg gtgctggata
      961 cctcggacac agcagaattt acatataata atgagacgat ttacacggca gatacggcat
     1021 tatctgatgt caagagtgct gccgggaaat ttatccggga tatcagtgca acgggcggag
     1081 ataattatgt ggcggtcatt tcttataaag attatgcaac tactgtatct ggtttttcaa
     1141 aagaatacag ctcgctgatc aataagatta ataatctttc cgcttccagc acgaccagag
     1201 atatatcaag tggtctggaa ctggcaaatt ctatgttaaa tcatgcagat agtgaaaatg
     1261 tgattaaaaa tgttgtgctt ttttctaccg gaatgaccaa cgaaggcgat tataattatg
     1321 atggttatta cgatggaaat gttgtgggaa atgcatggca cagaaatgat acaaatgttc
     1381 acctgtatgc atatgcaaat catacactgg aagaagcaga tctgttaaaa gatcagggaa
     1441 ttaacctgta ttctatcggt ctgttcaaga cgatggcaaa tatgccgcaa gaaggaaaga
     1501 atattgcaga atttttcaaa atgactgcgt ctgatatcgc aacatccgag gattattttt
     1561 atccggtgta ttcggtggat gatctggaat ttacgtttgg agaggtcgca gatgatattc
     1621 tgtcttcggt aaaggagata acgtttacct atagtggaga cagtacagcc aaatgttatt
     1681 attcggacaa ttattttgca aagagtgcct ataattatag tccgagtctg gcaacgatgt
     1741 cgctcagttt tgcaatgtcg gcatttggaa gcagtgatgg aggtcagacg gattacacca
     1801 ataaaagcag caatgccaga gcgttgttga aagaaatggg atttgcagat gaaaatatag
     1861 cagtaaatga ctggtttacg aagaaaccga cgacggattc gattggcgtt atcatcggaa
     1921 ataaacctgt gaaagtaaaa gatgaggaat atacactgat tgcagttgct gtcagaggcg
     1981 gcggttatga gcaggaatgg gcaagcaatt ttacgattgg tacatccgga cagcatcagg
     2041 gatttaatac tgcgaaaaat aatgttcttt cttatctgaa acaatatatt tccaagcagg
     2101 gaatttccgg acaggttaaa atatgggtca cgggatacag ccgtgcagcg gcaacagcga
     2161 atctggtgag cggtgaactt gataagggaa tcgctttggg aaacgatatc tcgtatcaga
     2221 gaaaagatgt ttatggttat tgttttgaaa cgccggcagg tgcattgagc gaagaggtaa
     2281 atggggattc aaagtatgat aatatattta atattattaa tcagagtgat cctgtgcctt
     2341 atgtagcacc ggcagccatg ggatttggca gatatggtat tgatcgatat ttgccgtctg
     2401 cggagtcaga accggaagat tatgcagatc tgaaaaagaa aatgctggcg atctaccagg
     2461 cgatgccaac aacagagaag tatgttgtag atgattttca aatgaaaaaa atctctgtgg
     2521 acaatctaac ctggaatgcg gttgggttct taaaagatgg gctgatcgtt aatgatacga
     2581 aacataatta ttctcagggc gtatttctgt cagattatgt tacaatctta tctaaaaaat
     2641 ttattgtaaa cagagaaaat tatgttgatc gttatcagaa tgagattcgg gagatttgtt
     2701 ccgtagtttt tggttgtacc gatgaacagt caggacgtct gacggattcg atcgtatcgc
     2761 aggtaaagag tgaatggtat aaatttgcgg gtgcatatat ctggaatacc gggatcaatc
     2821 catggggaac ggaagaaaag gcattgaaaa tcgtatcggg atggctcagg aaagcacttc
     2881 aggatgcagg aatcacagat tataatgagc ttgtcattga ttatgcgggt gtgaaactga
     2941 gtgatctgat gcttgcactt gtcagcaatc atccgaatta ttttacaacg gctgttttaa
     3001 atggagaagg actcggggca gctcattatc cggaactgtg ttatgcatgg ctggcatcga
     3061 tggacagcaa ttatgtcgga acaactgcaa atcgcctgaa caatggcgga ttccggatca
     3121 ttcgaatcaa ctgtgaggtt gatgttaagg tttttgatgc agaacaaaat aagattgcat
     3181 cgatcatcaa tgagcagtcg gatgaaacag gaagttatat tgcaggggtg gataacaatg
     3241 ggcagaaata tgttgtcctt ccggtggatg aaacttttta tgtggaaatg actgcacgtg
     3301 aaaaagacag cgtcaattac agcatcaatg aatacagtgc tctggccggt gagtatacgc
     3361 gaaacatcaa ttatttcaat attgagatgc aaaaaggaga agtggtggaa ggcgttctcc
     3421 cggcatacga taaagcggag ctggagaagg atactccgga aggttccgat gcggactaca
     3481 gactttatga tgcagatgga aacacaataa attcggattc ggatttgtcc ggcgacgatg
     3541 caagaaatgc atattttacg gtggaagcac ttgtatctga tgaaaaagcc ggagctgtga
     3601 ttggcggagg tatttaccag tatgggcagt ttgcgaaact tcaggcaatt ccggaggaag
     3661 aatatgtttt cgaaggatgg tacagaaaag gaatactgct ttcaaaagaa gaggattaca
     3721 ggatacagat actgtctgat gaaagcatta ctgcgaagtt tgtgaaaaag cggacaccaa
     3781 aagtggtaaa actttcaaag acgaaatatg tatacgatgg aaagacaaaa aatccaggcg
     3841 ttattgtact tgatggtgat ggcaacagag tatcttccga tcattatacg gtatcgtatc
     3901 aggcaggaag aaagaaggtt ggtcagtacc atgtaaatgt aaaaatgaaa aataaatact
     3961 gtggcagcaa aacactttct ttcaaaattg ttccgaaagc gacgaagatc accaaagtca
     4021 tcaaaaagaa gaaggtactg agtatttcct ggaaaaaaca gaaaaagcag gtgtcaggat
     4081 atcagattca ggtatcaaca agcagaaaat tcaaatcaaa aaagacaaaa aatatcagtg
     4141 gtatgaaaaa gaacagtact acggtatcca agttaaaatc cagaaagaaa tattatatcc
     4201 ggatcagaac ctggaaaaag aaaaacagaa agaaatacta ttctgcctgg tcaaaagtta
     4261 aaatcggtaa gaccaaataa gagtatcggg gagccgcaaa aaaacagcgg cttccttttt
     4321 ggatatttcc ggggaaaaag aaccggtcag acggaaaaat tgcgaaaacc ctcggtttga
     4381 caatgtattt ttggtgtagt atactttaaa aacatgaaga gtaatagtag aataaaatag
     4441 gataataaca ggcaggaaca tatacgaggt gaaacagtgg ataaatacga atacaacctt
     4501 aagttagaag aaataacaaa gctggctgat cagggatatt atgaagatgc ggcagatatt
     4561 attgatacga ttgactggcg gagagtaaga aatgtcagaa cgctttgtat gatcagcgaa
     4621 atttatgaag aagtcggaag attgcaggac agtaaggcac ttttgcttcg tgcataccgc
     4681 aaatctcagg cagtgagaag cattttgttc catttaacgg agatttccgt aaaactgcgg
     4741 gagtttgatg atgcagcgga attttacagt gaatttgttg aagcagcacc gcatgacaat
     4801 tcacgttatc ttttgaaata tatgatctat cgaggcagag gtgcggcgat cgaggatcag
     4861 attgagattc tggaagaata taatcagaaa gaatacacgg aaaaatatgc ctatgagctt
     4921 gcaaaacttt attatcgtgc cggacagaag gagaaatgtg ttgatgcttg cgatgatctg
     4981 gttgtgtggt tcagacaggg aaaatatgtt atccgtgcac tcgaactgaa acagaggatc
     5041 aaagcactga caccggtaca gcaggcgatt tatgacaacc gcaacgaagc taccatgcca
     5101 aaagcggaaa tggtagaaaa tgttgcaccg gatctggaaa aagctattct ggaaaatatg
     5161 ccagaatcca gcaaagaagt gatcacggat gatgtgattt ctgtaacgga aaaagagatc
     5221 gcacagagtg tcgcagaaca tacagctgaa aaagaagaaa atgatgatta cggaatcttt
     5281 gcagcatttt ctgaaaatca ggaaaaaacc gatcaggaag aacaggattt ccataaggaa
     5341 ttgccagtaa cgaccccgga aaagactgtc gataatgaga atgccgcaga gaaggcagcg
     5401 gatgatgcag aggttgatga gaaaattatg gatgacgcag attccgaagc ggaaaacgta
     5461 tcaaaagaaa aggcagatgc agccggtcag gatgcaccgg caaaagcgga taaaaaatat
     5521 gaatttaata cagaagacct tcaggcagaa cttgcaaaga gtatgcggga nnnnnnnnnn
     5581 tattctctcc ggtattcaga gaaaaccgga tgagagtgat attatcgaac cgatgaatga
     5641 tgtgactgcc gaggcagaag aagcagctga agaagttccg gcagaagaga tcaagagaaa
     5701 caatattgat gatattctgc tgtccatgag ccttgcacag aaagaagcgg caatcgcagc
     5761 agttctgggc aaaaaggttg aagcagaacc acagccagtg aaagcagcag aatcacatgt
     5821 ggaggaagtc gctgaagttg agccggaaac agaggttgaa ctggctgaga aaatagttga
     5881 ggttgcacca gaagttgaac cacaggttga agaagtggct gaggctgaac cagaagaggc
     5941 agcaccgatt gagaaagtaa ttgaagaagt agcagagcca gaagagacac cacaggttga
     6001 agaagtagtt gag
//
LOCUS       DAWDWH010000126         5918 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5798_length_5918_cov_10.996248, whole genome shotgun sequence.
ACCESSION   DAWDWH010000126 DAWDWH010000000
VERSION     DAWDWH010000126.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5798_length_5918_cov_10.996248"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4736..4788
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1880 a         1108 c         1514 g         1363 t
ORIGIN      
        1 gggaacgatc acagaatccc agaccgagga caaggactgg atcaacaact ggaaacagta
       61 tttccaccag ttctatgtgg atgatatcct gatcatcccg tcctgggaag aagtaaaacc
      121 ggaagataag gataagatga tcattcatat tgatcctggc acggcgtttg gtaccggtat
      181 gcatgagacg acacagcttt gcatgcgtca gctgaaaaaa tatgtgaagg acggtatgga
      241 gatcctggat gtcggcaccg gaagcggtat cctgtccatt gcagcactga aactgggagc
      301 aggtcatgcg gtcggtacgg atcttgatcc atgtgcgatc tctgctacca gagaaaatct
      361 ggaagcaaac cagattccgg aaggcagcat ggatgtgatg atcggaaata tcattgatga
      421 taaagccgtg caggatcagg tcggatatga aaagtatgat attgtaacgg caaatattct
      481 ggcagatgtc ctgatcccac tgacgccggt gatctgccat cagatgaaac cgggagctct
      541 ttatattact tccggtattc tggatgtaaa agaagacgtt gtcgtaaaag cagtaaaaga
      601 tgcaggactg gaagttctgg aagttacaca tcagggcgaa tgggtttctg tcactgcgag
      661 aaaacaggca tagtaccgga ggaagattat gtacgatttt tttgcagact catctgcaat
      721 cggtgaaaaa gagatcatca tccgcggaag cgacctgaat catatgaaaa atgtactgcg
      781 ccttcagatt ggaggacaga tctgcatcca tgaggaaagt accggaaaag agtatcgctg
      841 cgaaattgcc ggatatgaaa aagatgtggc acatctgcac attatgtggg tggaagaggc
      901 aaaccgtgag ctgccatcga aaatttatct gttccagggg ctgccaaagg gcgataaaat
      961 ggaactgatc attcagaaag cagtagagct tggtgtgtat gagatcatcc cggtggcaac
     1021 gaaaagggca gttgtaaaat tggatgcaaa aaaggcggaa gcgaagcaga aacgctggca
     1081 gagcattgca gaaagtgcag ccaaacagtc caggagaaat atcattccgc atatccacga
     1141 ggttgtgaaa tttggtcagg ctgtggatta tgcaaaaaat ctggatatca cgctgatccc
     1201 ttatgagctg gcggaagaca tggaaaagac aaaacagata tttgagacaa tacagccggg
     1261 gcagtctatc ggtattttca ttggcccgga gggcggattt gatgaaagtg agattgcact
     1321 ggcacaggaa aatggcatca gcccgattac actgggaaaa cggatcttgc ggacggaaac
     1381 tgccggtatg gcggtgcttt cggttctgat gtttcagttg gagggcagag aataatgcag
     1441 gcatatttgg ataattcagc aacgacaaga tgttccgaag gtgtgcgtga catcgtcgta
     1501 aaagtgatga tggaggattt cggaaatccg tcatccagac acgacaaggg aatggaggca
     1561 gaaaaatatc ttcgcgaagc cagaatggac attgcggcta ctttaaaagc agatgaaaaa
     1621 gagatttatt ttacttctgg cggaaccgag tcagacaact gggcactgat cggaacggcg
     1681 atggcaaaca aacgtgccgg aaaacacatt attacaacgg cgatcgaaca tgctgccgtg
     1741 atccagccga tgatctatct tcaggagcag ggatttgagg tcagttacat accggtagac
     1801 cgctatggaa gaatagatct tcagaagttt tccgatgcga tccgggaaga tacgatcctt
     1861 gtgtccacca tgtatgtcaa taatgagatc gggactctgg agccgatcga agagatcggg
     1921 aagatcatca aagaaaaaaa tccaaaggca ctctggcatg tggatgcaat ccaggcctat
     1981 ggaaagtacc gtatttatcc gaaaaaactt ggcattgata tgctctcggt cagcggacat
     2041 aagctgcatg gaccaaaagg agtcggattc ctttatgttc gaaacggagt gaagatccat
     2101 ccgctgatcc tgggcggagg ccagcagaaa gggatgcgtt ccgggacaga taatgtgcct
     2161 ggaatcgccg gacttggttg tgcggcaaaa gaagcctaca cagattttga taaaaaagtg
     2221 acacatcttt gtgagctgaa acagtttttc atggaagaag tgcagaaaat accggatacg
     2281 gttctgaaca gccttccggg ggatcaggga gcaccgcaca ttgtaagtgt gagctttgca
     2341 ggcgtgcgaa gcgaagtgtt gctccatgca ctggaagagc gggggattca ggtatcttcc
     2401 ggttcagcat gttcttccaa caaaaaactt ccggtcagtc cggtattaaa agaaattggt
     2461 ctgaaaaaag atttgctgga ttctaccgtg cgattcagtt tctgtgagaa tacaacaaaa
     2521 gaagagctgg aatactgcct gcagcagtta aaggaactgc ttgtggtgct ccgcagatac
     2581 agcagacatt aaaagatatg atgtaactgt gaatatacga aacaactgcg atacaggaaa
     2641 agaaaaggag aaaagaaatc tatgtttcat gcatttctaa tcaaatacgg agagatcggt
     2701 atcaagggaa aaaaccgtta tctcttcgaa gatgcactgg taaaacagat tcgccatgca
     2761 ctgaagaaag tggaaggtga atttaatgta cgaaaagaac agggacgtat ctttgtggag
     2821 agcgaaggcc tgtttgattt tgaagaagta gtggaggcac tgcagagagt tttcggtatt
     2881 gtgggtatct gtccggtact gaaagtaaat gatgaaggat ttgatcagct ttctcaggaa
     2941 gtgatcgatt atatggcacg cgtatatccg gatggagagc atacgttcaa agtcaatgca
     3001 agaagagcaa gaaaaaatta tccgaaaaat tccatggaat taaatgcaga tcttggcgag
     3061 cgtattctgg aagcgttccc gaatatgagc gttgacgtac atcatccgga aatcaccctg
     3121 aacgtggaga ttcgtgaaaa gatctatatc tattcaaaga tcattccggg acctggtggt
     3181 atgccggtcg gaacaaacgg aaaagctatg cttctgttat ccggtggaat tgacagcccg
     3241 gttgcaggct acatgatcgc aaaacgcggc gttaagattg atgccgttta tttccatgca
     3301 ccgccataca ccagtgagcg tgcaaaacag aaagtggttg acctggcacg tctggtgtcg
     3361 aaatacagcg gaccgatcgt tctgcatgtc gtaaacttta ctgacattca gctgtatatt
     3421 tatgataaat gccctcatga tgaactcaca atcattatga gacgttacat gatgcgtatc
     3481 gcagagcaca tcgcgaaaga gaccggatgc ctgggactga tcacaggaga aagcatcgga
     3541 caggtagcaa gccagaccat gcagagtctt gcagcaacca acgaagtctg cacccttccg
     3601 gtattccgtc cggtgatcgg ttttgataaa caggagatcg tggatgtatc cctgaagatc
     3661 gatacttatg agacatcgat ccagccgtac gaagactgct gtacgatctt tgtggcaaaa
     3721 catccggtta ccaaaccgaa tatcaaagtg atcaaacgca gtgaagagaa actgcaggaa
     3781 gagatcgacg agatggtgaa aaaagcaatc gatacggctg agacactctg ggtagaataa
     3841 aaagacacgg aaaaatggtt acaaaaaagc tgccgcagga gaactgcggc agttaaaaat
     3901 atcaaaatca aaagaaatcc gaaattttat gtggtttttc agtcgatgat gtatggtttt
     3961 aatgcctgaa gaattacatc ggaatcctct tcagcatgga tattcaggtc gatcggcttg
     4021 gaaaggtcca gactgaaaat acccataata gattttgcgt cgattacgta tcttccggat
     4081 accagatcaa aatcataatc aaattttgta atttcgttta cgaatgactt tactttatca
     4141 atcgaattta aagaaatctg tacagttttc attgtcataa aacctcctgt aatatgtgtt
     4201 tttgtataca ttaatcatac ccatttcgga ggagactgtc aagcttaaaa gaaaggaatt
     4261 ggataaaata tggaaaaacg caaagcggca ttccacaatc tgggatgcaa ggtaaatgca
     4321 tatgagacgg aggcgatgca gcagcttctg gaaaaagcag ggtatgagat tgtgccgttt
     4381 gcaccggggg cagatgttta tgtgatcaac acctgtacgg tgacgaatat ggcagaccgc
     4441 aagtccagac agatgctgca caaagcaaag aaaatgaatc cgcatgcgat cgtggtggca
     4501 gccggatgtt atgtgcaggc agcaggaact gagctggaag aagactctgc gatcgacatt
     4561 attatcggaa ataaccgcaa aaaagatctg gtcagtattc tggaacatta tgaacgcagt
     4621 gccgtgcagg aatctctgat cgatatcaat cacacaaaag agtacgagaa actgtctctg
     4681 gaaaaaacgg tggaacacac cagagcctat ctgaaagtac aggatggctg taatcnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg catgaggttg
     4801 cgggggtcga gcgtattcgc cttgggtcac tggaaccggg tatcattaca gaagattttg
     4861 tgaaaacact ttctgctctt ccgaaattct gcccgcactt ccacctttct ctgcagagtg
     4921 gatgtgatgc aactttaaaa agaatgaatc gaagatacac atcaggagaa tactatgaaa
     4981 aatgcatact gatccgcaag tactttaaac atccggccct gacgaccgat gtgatcgtcg
     5041 gatttccggg ggaaaccgag gaagagtttg caatcaccag agatttctta aataaagtag
     5101 atttttatga aacgcatatt ttccagtatt ccagaagaaa aggaacgaag gcagccgtca
     5161 tggaaaatca ggtgccgtcc gatgtggcac acagaagaag tgcgatcctg atcgcagacg
     5221 gcaaggtgcg gaagcagcga tttgaagaaa aaatcagcga aaccgtcaca aaagtactct
     5281 ttgaagaaag cgtgacgatt gacgggaagg tatatgcatc cggccatacc atggagtata
     5341 ttcgcatgtt gatggagacg acggaaaatt tagacaatca gattcttttg gtaaaggcag
     5401 gaaaagaacg ctgtcaggac tgtctgctgt gcacaaaagc tcggtcctga gcagcggatt
     5461 gttttcaaag ccattataaa aatcacgcga agaagcatta attacaaaaa acaattgaat
     5521 ttttcagaca gttatattag aatagaagta tagtttgtac ggtaaggacg tgaaaagaat
     5581 ggcaaatatc agtaatacac aatatttcaa agtaaataca gatccggaat atcgcgtaaa
     5641 agagatcctg gatactgttt atgacgcaat ggcagaaaag ggctataatc cggtgaacca
     5701 gattgtaggc tacattatgt caggcgatcc tacttatatc accagttata aaaatgcaag
     5761 aagcatgatc atgaaagtgg aacgtgatga gctggtagaa gaacttctga aggagtatat
     5821 taaaaacaga acatgggaac agaagtaaag aaaagagcac tcggactgga ttacggctcc
     5881 aagacggttg gagtcgcagt cagtgacggg cttgggat
//
LOCUS       DAWDWH010000127         5865 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5846_length_5865_cov_15.738382, whole genome shotgun sequence.
ACCESSION   DAWDWH010000127 DAWDWH010000000
VERSION     DAWDWH010000127.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5865)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5846_length_5865_cov_15.738382"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3527..3536
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1124 a         1484 c         1208 g         2039 t
ORIGIN      
        1 gtgccatgat gcgggaactg aaaaaattaa aggcggaggg aattgtaaaa tcagaacgca
       61 aaacgtttac cttagtcgaa tatcgtcaaa actgaaccgg attatatacc ctgttttacc
      121 ttcttcctct gaaaaatgca ccttaattga ttcacgcatc aaaatctgct tcaaacacac
      181 cttccggtgt ccgcatggtc agaattccga ggttaaaatc ctcatcctgg atcgtgatgt
      241 ggtattcaaa caccacatcc tcctcaaatt ttgacagaag cagataggtt ccgttctcct
      301 ttccgtcaat ctggtcgcag atgtcattgt atacatcttc tccggaaatc gctctcggat
      361 atccggaggc tttgattttc tgctcaatgg cttcatacag ttcgttcata tgctcttcct
      421 ctctttctat attggatgcc acgtaaagcg gacattcaca atccgcttcg ctacttgccg
      481 agtaagtggt cgctgtgaca attgtaccat ctttccatct catatggtac tgtcattttt
      541 gtccttcttc gtaagagtct ctatgcagct tgatgataat ccatcactgc gatttcctgc
      601 attttattca tctctataaa gagagacacc gtgacggaaa ctttaccgtc acggtgcctc
      661 tctcttttat gaccagtatt ctatggaaca cctcataatt ttatttctta ttttgtgcgt
      721 actttctttt ttgcactgta agcggtgtaa actttctcgt tatccactac tttgtatgct
      781 ttgactctta catagtacgt tttctttgcg gaaagtctct tgatcgtttt tgcttttttg
      841 gaagcggaaa cggtaacggt ctttttgcct ttgaagtttg ctttcttagc gtactcgatc
      901 acgtatccgt ctgctccttc tacgctcttc cagttgactt tcacagactt cttaacacct
      961 ttgacagctt ttactttcac tttcttagct gcaaattctg tcttagtcag ctggctctcc
     1021 agacgtttct gcattgcctg cacttcttct aactgtttct gtgcttcggc tctcttttct
     1081 tccgcctctt tctgggcttt tgcaaggatc tcttctgctt tcttctgtgc ttcttcggct
     1141 gctttcgctg cggcttctgc atttgcctga gctgctttcg ctgcatccgc acttgccttt
     1201 gcagcacctg cggctgcctc tgcaaggcct ttctgtgcac ttacggagtc tttggctgct
     1261 tctgctgctt ttgctgcggt ctcagcatcc ttttttgcat tttcagctgt tgttttggct
     1321 acctccgcgg ctgtttttgc tgcttctgca tcgtttgcat aattctttgc tgcatcctct
     1381 gcttcttttg cggctttctg tgctgcctct gcttttgcct gggctgcttc ggaagcggtc
     1441 tgtgctttct ttgcctcttc ttctgcagtt tctgctgctt tctgtgcaac ttctgctttc
     1501 gcctgagctg cttctgctgc tgctttgtca acatcggctg ctgcctttgc ttcttctgcg
     1561 gctttctttg cttcttctgc ggctgctttt acttcttctg cttttgcctt tgctgcttct
     1621 gcatctgctt ttgctgcttc tgcggctttt gctgcggtct cagcatcgtt ctttgctgcg
     1681 actgcctggt ttttgatttc ttccagttcg tctttggatg gctgatccgg tgtgttatcg
     1741 tcttctttta cataaacttt tgcatctgca atgtctacgc agtcatcatt ctttccgccg
     1801 actttattga tgtagatgat cagtttctct acgcctgtca gatcaacgtt catcagtttt
     1861 gcattgtccg gctgaaggat cacaccgctc tctgccagtt tattctcttc gctgatactg
     1921 tctttataga agataaagtt tgcaccatca cgatttgtct tggttgattt tgtatagtcg
     1981 atacccacat aaccttcaaa gcgttctgct ttgataccgc ttaaatcaaa ggtcagagtt
     2041 gcatcggtat ttgcacttaa gccctgtgca aagactttgc gtgcaccgtc aacgttcatg
     2101 ctcagttcgc ctttgtttgt acttgtgccg ccatcgtact cgttgacatg gaatccgccc
     2161 catccggatg aggaatttgc cacgtccatg tataacagac cgtatttatc ggatttttcg
     2221 gtatcggttc cgtatactcc gtttgcttcg tctacttttg cggttaagta tacatatttc
     2281 tggtcagtag acggtgttac atcttttccg cctgccggtg ttacttttac ttcttctgcg
     2341 gaagcatcga tctgtgcatc ttttccggaa atcgtcagat tttcaatgga gaaacttgtc
     2401 tctttctttt ctgcattttc gttcaggttg aagcttgctt tgaacagatc tgcctgtgct
     2461 gcaactgctt ttgtcagatc gtaaactacc tgcacaccgt ctgctgtttc ttttacggaa
     2521 atatttcctt ccacaccgtc tgccagtgcg atgctgttaa atttataagc tactgtgtcg
     2581 tatttcagtg tgaagcttcc tgccatcatt gcagaactgc tgctctcgga tgtctgggaa
     2641 atgcttcctg tcacttctac agttccgttt tcctgtgctg tgttatcttt tgccgttaat
     2701 tttacggttg ctgcttcttt tgcaccgtcg atgtctgttc cgattcctgc cagtactttc
     2761 agtgctgcca gatcggcaag atctactttt ccgtcaccgt ttacatcggc tgcataagta
     2821 ttgactgcat cgaatgttgc attgcctgcc agtacttcac ggagcatatt gatatcagcg
     2881 atatccagtg ttccgtttgc acttccgtcc acatcaccgt ttactgccat ggttacggta
     2941 tctgctaccg taccgtcttt gcgaagtttt acgagatatc cggttgctac cggtgctccg
     3001 tcttcgtcaa aggatgcatt gtacggatct tccattgata aggtttcacc ctcgccaagc
     3061 tcaaacatgg taaagagctg tgcttttgtc atgcctgccg gaacattgta aagaatattt
     3121 gcagaacgat ccagtttgac tgttgcacct tcttttaaag tcagggatgt acctgtctct
     3181 tctccggtgc tgagctgaac tttggtatca caccagctta tcatatcgtg tccgtctgtt
     3241 acgtttttgt ctgcatacag gcagatttct tttgttcctg ccgggatctc gatgtcgaag
     3301 tactgtgcgt tctgaccatg tttcatcagc tcgcctttcc atacttcctt gccgtccagc
     3361 aggatgctcg gataagctcc gtcctttgtc tgtgttttat ctgcatcaat tccgacatag
     3421 ccctgaaggc gtgttgcgtt cagtttggaa atatcaaagt aaagtgctga gtccgcattg
     3481 ataccgaagc ctttttcaaa tttcgtgcgt tttccatcca catacannnn nnnnnncgtt
     3541 cctttgttat cctcgtttac atggattccg ttgtatccgg aggtacttct tgcggtatca
     3601 tatccgagat cggaaagctg aacggaagta tcgttcttga tctcgatcgt ataagttctg
     3661 ctgaatccat tctttgcggt tgcttttaca gttgctgtgc ccggaaggct ttccggtttt
     3721 gtgatctcaa cttttacgtc ttcggatact gcttccgctg tgatctccgg aaccgttccg
     3781 gacagggaag aaacattcat gctgtaggaa gtgatatcat tgtcaaatcc ttccagtgcc
     3841 tgtccgccga ctttgatgcc gttcagcagt gcggtttcat ctgcataatt ctttttgctc
     3901 tctgtcatca gctcttcgct tgtagatttg ctgatcggtt tgaggcggta agtatagctg
     3961 taagttttac ctgttccaag cagatatttg gaaagggttg cctgtccgca gcttgcggtt
     4021 ccaagtccca tctgtgcata atcgatcgtc agaacggtat tgtctgtgcg ttcgatctga
     4081 tacggatgtt ttgctgcttc cagtgcttcc tgtgtgcagt tcagtgcact catctccagc
     4141 aggtgatcct gtgcgtcaac cagcagtccg tcaccgtctg catcggttac tgccatccat
     4201 ctgacatcgg tcttgtttcc ggtttcctga gcgtaaacat aattggtaaa ctgttcatct
     4261 acggtgctgt tccatacacc gacatcgtat ccggtctttc tgtcgctgta gctgtccgac
     4321 tcgccgcggc cataccaggt caggttgtcc agtccggctg ccagctgcag gcgtgtgccg
     4381 actctctgaa ggttgctcat gttggagttc ggagccatgg ttgcttttac aacgatctca
     4441 ccgtttgcat agattgcata agtgatgcgg tctttggaat ccgcacagtt tttcagtgta
     4501 cgtgctacgg aaacataaac cactttgttt tctttattaa cgctcacttc atcgatctga
     4561 acgttctcat ttgctgtttt ccattttgca tccacggatt cttttttatc attatccgta
     4621 gaagctctcc agtagttcgg ctcaatgcct tctgcaatca tttcttttcc gtttgactga
     4681 taagaagtca tctcaccggt tttcttatca aaggaaaccg accagttatc gcctgctact
     4741 tttacatcac catcggtttc ttcaaatgtc ttgatatctt ccagtttgga agtgtctacg
     4801 gcttccactg ctgcatcggt tcctgcgaaa tccagttcaa actgtgcttc tgccactgca
     4861 aaatctttgt ctgcccaggt gaactgatcg tcactctttg ttacgaatga aatattcagg
     4921 aaatattcat cacctgctgc cgcatcggtc ggtgtggtga acgggatcac aacatctttg
     4981 ctttcacctg ccgggatatc cacatccatc gttccatttt ctacggaagt tccgttcttc
     5041 acaacttccc atttcatggt atattcattt gcattggtat cgatccattc gttgctgatc
     5101 gtaacagtcc tggaagccag atccttgagc tgcatctgca gtgcctggta aatacgcttt
     5161 acttcataag cttctggctg gagcgtacgg tccggagata cgataccgtt gacacagaag
     5221 tccgtatttc cagatttcca ggaagaatag tccttggtat ttccgtctgc atcggtgtaa
     5281 gttccgtggt tccagtcacc gtcatagccc cagtaagtgc tctttccatc ccagtttccg
     5341 tcagaatcca gaatcggtgt attatagctc tgatctacga aatcccagat aaatccaccc
     5401 tgcaggatgc cgttggcacg gatttcttcc cagtattctt tcataccgcc gcagctgttg
     5461 cccattgcat gcaggtattc acacatcaga tatggtttct ttgaattttt aacctgtttt
     5521 ccaatgttcg gatacatgtt ggaccataca tctacgcctg cattatcatt ccagccctga
     5581 tagtgaacga ctctggtgtc gtcgttattc tggaaataat caatttctgc ctgaagggaa
     5641 tcaccggctc ccgtctcatt tccaggtgac cacatgataa cacatggata gtttttgtca
     5701 cgctccagca tattgatggc acggtcttct gctgcctgaa catatcccgg aaggcttccc
     5761 ggcactttgt actggcttcg tccgttgtga gactctacgt ttgcctcatc catcacatag
     5821 ataccatact cgtcacagag tttgtagaaa gtcggatcat tcgga
//
LOCUS       DAWDWH010000128         5844 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5871_length_5844_cov_12.272240, whole genome shotgun sequence.
ACCESSION   DAWDWH010000128 DAWDWH010000000
VERSION     DAWDWH010000128.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5871_length_5844_cov_12.272240"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2320..2329
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1343 a         1484 c         1123 g         1884 t
ORIGIN      
        1 tataaaattt tcaatgttct tcaatttagc cttgactttt tatttgcagg cttaatagtt
       61 tttgtgttca tcttggtatc atgatacctg tgaattgctc cgttataaag taacttccat
      121 ctgatatatt atacatagta taatatgccg ccgcaacgcc cgctttctta aactttattt
      181 aatttcttat tgcattcttc agaaaccaca gcttcccgga tacgcaaaaa caggagatgg
      241 aacatggaaa aacagcgctc cttctcctgc tttggtttta aaatatgatg ttatgtttca
      301 aagatgtcct gccattactt catgctttat agcttcagat ccggcatctc agatatttaa
      361 gaactgtttt acgatctttg gcatatcctc tgtctctacc acattcttgt gaagaaccgg
      421 tgcggtacgg atctcttcga tcgcctgcgg aactttcaca ccggacagtt cgctcagtgc
      481 gtctaccagt tcgaaatcac ccatgccttt gtacttttct gcatcaatgg catccataac
      541 acttctggtg aatttatacg gacttgctgt ggatgcgatc acggtcttgg tttgatcacc
      601 gctctctgcc acatacttgc ggtatactct ggaagcaaca gcggtatgtg tgtccataat
      661 ataacctgtt tttttgtaaa cttctgcgat ttcttcggca gtttcttttt catttgcata
      721 gttgccgtag aaatctttca gctgagcttt catctcatct gtaatctcat atttgccttc
      781 tgctgccaga ttctgcatca gagttacatc ttttgctgca tctgcacctg cgatcttgta
      841 gatcagacgc tccaggttgc tggagatcag gatatccatg gacggagatg ttgtcagaat
      901 aaactcacga tttctgtcat acgttccggt gcggaagaaa tcatagagaa ccttgttgtc
      961 gttggaagca cagatcagct tgtcgatcgg cacacccatg ttctttgcat agtaggctgc
     1021 cagaatattt ccgaagtttc cggtaggtac tacgacattg atcttttctc cctttgccac
     1081 ttcaccgttt gcatacattt ttgcataagc atatacatag taaacaacct gcggaaccag
     1141 tcttccgata ttgatggagt ttgcggaaga gaactgatat cctgccttgt ccatctgtgc
     1201 tgccagttct ttgtcaccaa acattttttt cacgcctgtc tgtgcatcat caaaattgcc
     1261 atggatccct actacaaaag tattggcacc tttctgtgtt accatctgct tttcctggat
     1321 cgggcttacg ccgtttttcg gatagaatac aatgatcttt gttccaggta catctgcaaa
     1381 tcccgcaagt gctgcttttc cggtatctcc ggatgttgct gtgaggatca cgatctcatt
     1441 cttcacctga tttttctttg cggatgtggt cagaagatgc ggcagaatgg acaatgccat
     1501 atccttaaaa gcaatcgtct ttccgtggaa cagttccaga taataagcct catctttttt
     1561 tacaagcgga gcgatctcct ttgtatcgaa tttctcatcg tatgcttttg cgatgcagtc
     1621 tttcagttcc tcttccgtaa aatcggtaag aaactgacgc ataacttcat atgcagtctc
     1681 ctggtagctc atccctgcaa gggtgtccat atctgcttcc agtgccggaa gactctccgg
     1741 tacaaacaga ccgccatcat ctgccagtcc tttcaggatt gcctgggatg cagttacttc
     1801 gacatcgtta cttctggtac ttttgtataa aagattcata acagccgtcc tttccttcaa
     1861 acgccatatt ttgcgataaa ctttacggga gtcaccttac atctgatgca gtctctccac
     1921 gcttaattcc agattataac ataccttttt tcactgtgca agagcaaagg aacgaagttg
     1981 agaaaaaccg gagacacttc ttaccatgcc cccggttttt tctcatgcag cggatcattc
     2041 atccgcaaaa cagaaaaatt cgtgtccgcc atattcaaac atctgcacca gattctggtc
     2101 aaaccagacc atattctggg cttccgcact ggcacgtgct gcaaagaaca gtgccccctg
     2161 cgaataatct tccccggcca atgcccggtc taccgcctcg atcgtatcat ccgtgatcga
     2221 gaccgtatag atcctgccgt cgtcggtagg ggaaaactgc ggcgaaccgc ccgccgtctg
     2281 ccagacaaca tcgtagatcg tatctggaaa tctggaatcn nnnnnnnnng cttctacgat
     2341 ctgaagaagt gtgtaataat cattcccatc catctgattt tccagcactt tctgctgtgc
     2401 ataatagccg atgcttccga tgttttttgc acactcctcc acgtacttct ggtagagggc
     2461 attttttctg attcgtgatg ttccggcaag gatctcctga ttcgccgtct caagatcaac
     2521 gccatccgct gcctgcgagt acgcttccag cttgtttacg ctggcaacaa ttcccatcaa
     2581 acctgcctga agctcctctt ccttcacatc ctcaccctca cctgcttctt ctgcatttcg
     2641 ggaggctttt acagcatata cctgattttt tcctcctccg gtaaaactgc ccgctgtaaa
     2701 aaccaataca gccaccacaa ctgtacctgt cataagaact gcacagttgc gataaatctg
     2761 tttgctgagt gtacgtgaaa aacatacctt aaaaaaacca cagatattat ctaagatcac
     2821 tcccagcagc cacataatgc cattcctcct tccgcagttg atgtacctgc attataagtg
     2881 aagaatttgt tacatgtcaa ggtaaatgtt acagattcgt tacattttac aacatatttt
     2941 tttcgatcac atacaatttt atgagatgtt tccataattt tatcacattt caaacattat
     3001 ttgttgcaat atgtataacc tgttttcttg atttttgtca ttttacccaa gtaaattttc
     3061 ggcatttttc aacttttgaa caatagatgt ttcattttcg tttactcaat tgttgattat
     3121 gtcattttaa catttttgaa atatttgtaa tattttgtag ttatccacat tttcctctga
     3181 gcagcttatt cactttctcc tttacaaaca aattcttcca ttatataatg actgccggga
     3241 atgaggttct ccctggtttt taaccaaacc gcttgtatat aagctgatga cttctgcgat
     3301 ttcgcaggat caccagcttt ttttatgcga gcaacgagcc aaagtccgtg cttgcacgag
     3361 accttggcga ctgccgcgat aacttgagaa acccacaaag cgtgtttctc atagtttcat
     3421 tataataaag gaggtcttta tgttaacatc acttgcactc atttttctga ccggcctgct
     3481 cctgtcatct ctttttgaga agctgaagct gccccgtatc atcggtatgc tgcttgccgg
     3541 cattttgctt ggtccgtatg tgctgaacct gctggatgat tccgtattat ccatatcatc
     3601 cgatctgcgg cagatagcac tgatcattat cctgatcaag gccgggcttt ctctgaatct
     3661 ttctgacctg aaaaaggtcg gtcgtcctgc gattctgatg tcctttgttc ccgccacatt
     3721 cgagatcatc ggatatgttc tgctggcacc gactttgctc ggcatttcca aaatcgaggc
     3781 agcagtcatg ggatctgtgc tcgctgccgt ttcccctgcc gtcgtcatcc cgcgtatggt
     3841 tcagctcatg gaaacgaatt atggcaccga aaaaagtata ccccagctca ttatggccgg
     3901 ggcatcctgt gatgatattt ttgtcattgt actgttttct acattttcga gcatggctca
     3961 gggcggttct gcaaacatca tggatttcgt aaacattccg gtatccatcc tgcttggcat
     4021 tgtgatcggt gccatcattg gctatctgct cgccctgttt tttgaaacct gccatagaca
     4081 ggggcatagc atccgcagca gtctgaaagt cctgatcctg ctcagccttg cttttctctg
     4141 catggcactg gagaaccgcc tcaaaggaat cgttgccatt tccggacttc ttgccgttgt
     4201 cagtatggca tgcgtggtaa agctgaaaag tcccatcgat gtcaccaatc gtctgtcact
     4261 gaagtttggc aagctctgga tcgctgccga gatcctgctc tttgtactgg tcggtgccgc
     4321 tgtagacatc cgctatacga tgcaggccgg aatcgccgca gtcgccatga tcctgctggc
     4381 tcttgttttc cgtgccgtcg gtgtctgcct gtgcatgaca aaaacttcac tgaatttcaa
     4441 agaacgtctt ttctgcgtga tcgcctatct tccaaaagca accgttcagg ctgcgatcgg
     4501 ttccgtcccg ctttccatgg gacttgcctg cggacagatc gtgctctctg tcgcagtgct
     4561 ggcgatcctg atcaccgcac cgcttggagc gatcggtatt gaccggactt acaaaaagct
     4621 gcttgttatt actcaccatt tgcagaagtg ggagtcttct cgactgagat aaactctgca
     4681 gattttatac ccgttctgct ctctgtcgta cagaagcatc cgatcgtccc tgcccatctt
     4741 gcgttcttct tccagaacgc tccggattgt ctccagatcc ctctggatcg tccgctctgt
     4801 cacctgatag tgacgtgctt cctctttttt cttcaggatg tcaccatatg acagtctcac
     4861 aaacaggctc agaattctgg tatttctgcc ttccttttct cccatgctat cctccttcca
     4921 tatgatttga gagcagtgta gcatgttttc tctccggcgt tgtgaaaatg atttaatctt
     4981 ctcttttgaa ctcaatctcc ccatggcagt atgtatccac aaaccacgta agctccctgc
     5041 gaaagtctgc tgctttgctg ctgttccaga aatctttcat ctcatcatac tgaagctcct
     5101 tctgaatatt tgacaggtga tcaatctgct cttctgtcac actttgatgc agtgccgtct
     5161 caaatgtttt atataatagt ttccattctc cggcaaagta ccgatccagg ctgtcaaaaa
     5221 acggtgcatc ctgctgatct tccacggata cgatcacgga catgctgtaa aatacaaacg
     5281 cttcctgctg cagtccgatc tctgccagaa tgtctgcata gtgttccaga tctcttgaaa
     5341 aataatattt tctcgtttct tcatcataat ttggccaggc atcctctggt tttggagtct
     5401 gatactcatg cagggtcagc gtgtatgctt tccggatgat ctgaagtaca ttctgaaatt
     5461 gtttctgtct gcccgcacac cttgcacgat cctctgcaaa ccatttatat tgcttatagt
     5521 ccggctctgc cccttctttt tgcatcctct gaaataaaag ttccatctgc tgataacact
     5581 tctcttccat cggatcttct tttctgtcac gataacttct tgctgcatct tcccagattt
     5641 ctaactgttt ttctgttttc tgacctttga catccgtttt tgccaccaga aaataatcgc
     5701 atttcttctg caatttttct gccctttcat ccagcacact gtatttttcc cgataacagg
     5761 atgcaagtat tttgtatccg agacttcgca gataggcatc ctgatttttc acctgtttct
     5821 gacgtttcag agctgcaaaa gcat
//
LOCUS       DAWDWH010000129         5798 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5913_length_5798_cov_11.506878, whole genome shotgun sequence.
ACCESSION   DAWDWH010000129 DAWDWH010000000
VERSION     DAWDWH010000129.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5798)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5913_length_5798_cov_11.506878"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4933..4942
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1401 a         1392 c         1067 g         1928 t
ORIGIN      
        1 caccaagaac cggaatatga gaaacctgct cgatcatgtc tgtaaatatc gggctggatg
       61 ccaacggttt catgacgctt cccatcgtct cgattccaag gaacagaagg ccaaatgcaa
      121 aaatggtttg tccgatgttt tttgcacgtt cggattttac tacaaaacta atgataaatc
      181 cgataaaaat aattgcataa atataatcac ttaacttaaa ggcaatgatc tgtgccgtca
      241 tcgtcgttcc gatgttggct ccgaggatga tggagattgc ctgcggcaga ttcatcagtc
      301 ctgcacttac aaaaccgatc gccataacgg ttgtcgcact gctgctctgc agaacggcgg
      361 ttgtcagtgc tcctgccaga acgccaagga ttggattctt ggtaagcaat gccaaaatcc
      421 gtttcatttt ttcgcctgct gccttctgaa ggcagtcgct catcatattc attccgaaaa
      481 tgaacactgc cagaccaccg agcaatccaa atattacctc taatgtactg ttcatgattt
      541 ctccttcttc gctttacata cgtttttcat ttcagattcg ttctttattc gggatgcagc
      601 acattttttc aggggcttgg aactttcgcc ccgcaccccc tgcatcacca aagcatctac
      661 agtataacga tgcaatgtaa agtccacgac agtgtaatgt aaaatctgtg taaagtctcg
      721 taaggacaaa gccactgttc ttcaaaattt ttcttatcaa gtttatgtaa aacaggcaat
      781 ggtatgcttc tgtatacatc aaaaagatca cataccaaaa tgcttttata catttcagta
      841 tgtgatcttt aaggtatgta tatcttattt caagtcgaac gtacgtgaga tttggcaggc
      901 aattctggtc agctttgctg acatttatct actttgcagc aatatctgtt tttacataat
      961 tttgcggaac agatctttca gatcttctac gcttgcatct ttgggattgc ccggtgcaca
     1021 agcatctgca tgagcagatt ctgccaggaa atcaaggtct tcttctttga ttgcttccag
     1081 ttttgtcggg attccgacat caacagaaag ttttcttacg gcatcgattg ctgcttttct
     1141 gtattcttct acggacatat catcaacacc tttgactccc atcgcacgtg cgatctcacg
     1201 gtatttctcg cctgtgcagt cctgattgta ttccataacg atcggcagca tcattgcaca
     1261 tgcaacaccg tgcggtgtat cataaacagc acctaaggta tgtgccatgg agtgtgcgat
     1321 tccaagtcct acgtttgaga atcccatgcc ggcgatgtac tggccaagtg ccatgccttc
     1381 gcggccttcc tttttgtttt ctactgcatc tcttaaagaa gcggagatca gtttgatcgc
     1441 ttccagatgg aacatatcgg tcatttccca tgcagctttt gtagtatatc cttcgattgc
     1501 atgtgtcagg gcatccatac cagtcgaagc agtcagtcct ttcggcatgg aggacatcat
     1561 ttccggatcg atcactgcga tgatcggcat atcgtgcggg tctacgcaga caaatttacg
     1621 tttacgctct acatcggtga taacatagtt gatcgtaact tctgctgctg ttccggctgt
     1681 ggttggtacc gcaatgatcg gtacacatgg gtttttggtc ggtgcagttc cttccagact
     1741 tctgacatct tcaaattccg gattggcaat gatgataccg attgcttttg cagtatccat
     1801 ggaagaaccg ccgccgatgg cgatgatata atctgcttta gaatctttaa atgcctgaac
     1861 accatgtttt acattgtcga tggtcgggtt cggcttgata tcagaataga gctggtatgc
     1921 aagtccggct ttttccagca gatctgtcac ttttgaagta acgttgaatt taaccagatc
     1981 cggatcagag catacaaatg ctttctgaaa accgtgtgcc tgtgcttcgt ttacgatctc
     2041 tgtgatcgca cctgcaccat gaaaagaggt tccgtttaag ttgattctgt ttgccatagt
     2101 tattacatcc cttctgtaaa attttaacgt ttccggcaat gattttgctg tatctatgta
     2161 gctgtgcatc atcattccgt ttcactttgt tattatctta acacttttgc acaaaaagaa
     2221 aagtgacaaa acagactaaa tgtaacattt atcaaaaata ctttgcaacg aaatcacagg
     2281 cagatatctg cctgtcagaa tccgagacaa tctattgttt acctgaccat atccagtttc
     2341 tcaaacagtt cttttagttt taccggcatc gcttctacca tcagttctgc cagtccttct
     2401 ggtgcggcca tctttttccc aagcacccat tttacggtca tatagacaga acttctgcaa
     2461 tacatttcca gcagatcatg catctcttct ttcattttac atccggtttt ttcctggatc
     2521 agattttcat aaaatgccag aatcagttca aaatcatgtt ccctcagaga attctgttca
     2581 tcatagcaga acgctgcagc aaaaaactgt ttctcctgct caatgtaggt aaatttccgc
     2641 acaagtgcat cgtaaacact tgtttctctt cccatatgct caaacgactg atccaggatt
     2701 tttccaaaat accagttgat aaggtcaaat ttatccagaa aattgcgata aaaggtctgt
     2761 ctggtcagac cacatttttc tgccagctgc tttactgtga tcgcatccac tttggaagtc
     2821 ttcatacatt ccttcatggc ttctgctaat ttatacttgg ttttttcgtt tttctccatc
     2881 tccattccct cgtcttcgaa tactgaggtg gcggctttat ctgtttttta cttttctgac
     2941 tgcagaccat gcaccgaatt ttttcgttcc gtttatcttc tgataagccc gtatcctcac
     3001 atagcatcct tttttatttt ttaatttctt attctttacg acgagttttt ttgcaccttt
     3061 gtttttcttt gcttttcctg aagtcggata gactttgatc tgatatccgg aagcttttgc
     3121 cacttttctg acaagcgtta tggtcactgt cggggacgcc tttttctttt ttgcactttt
     3181 gatactcgct tttccgactt ttatgctgct ttgtgtatcc ggctttctgt caggcgtctt
     3241 ttcatcatct tttccggttg tcgtttccgc ctttttctgc accactgtaa ctttgcagtt
     3301 cgctgtgata tttcccgcct cgcttaccgc tgtgatcacg gcagttcctg cggcatgtgc
     3361 tgtaatttct ccacattcat tgacggttgc gactgccgga tttgtgcttg taaaataaat
     3421 tctcttattt ctggcatctt ccggatgtat gatcgctgtc agtttttccg tgcttcccgg
     3481 cgtcagctcc atactgtcct tatcaaataa aatctgctgc ggttcttcct ttccatctga
     3541 agaaagatat actttttcat ataccgttct gtaagtatag gaggcatctt ttccatcccg
     3601 gtcattcaga tgctcataag tgatctcata gacgccgccc actttaaaag caatggaatt
     3661 gtccgcatat gagatcagcg taccattgac ctgatatgcc tgagaatcca gaagatttgc
     3721 cggatcaatt ttccagactg ctccactgtt cagacatctg accgtcactg tcgtatctga
     3781 cgtcggtttc agtccattgt aaaactggca tgttcacatt ccggacggtg caaaaccact
     3841 ctctgaaatg gtaactcctt ttgccggcca tgccacaact tctgcatcat aatcctgtgt
     3901 tcctgtcatt ttgtggcatc cctgctgcaa tgtatttcct acaccgatgt atttccatga
     3961 cggattcagc agattatagc gatgtcctct ggtataatat tgcccggttc cataactgtc
     4021 gtccagggca ttgctgatac ttccaatgat gtttgtgctg agaatgccac acataaacag
     4081 attttctccg gaaccgctct ggcactttgc atagtattca tcgcttaatc cgtccagttg
     4141 aggcggaaaa tgcggatttg gattgctgat cttgtttttg gacagataca ccgtataggt
     4201 tgatttgcac tgtgcatctg cggataactc ttctgaatac tccaatggat tcagtccacc
     4261 gcctgtgcgg atcgcattca gaaaatccac tgcccctttt gcaattcctt cgcttaattt
     4321 tcctccttct attgtatcca gagaatcata ggacggttct acttcataat aagatttttc
     4381 tgcattaaat tcttctactg attttgcata ataatctctc gcctgtttca gctgctgctt
     4441 ttcctgtgcc gtgtacgcta caggattcca ttctgccagc aattccttat tgacaaatcc
     4501 attgatcacc cagtactggc tcttgtcgct tccttttgga taatacatga gtcctatcgc
     4561 atttccattt ttacaggtat agtttttccc tttttctgca aattccagcg gatttggata
     4621 gcgatattcc gggaaactga tcaagctgct tcccatcagc tgatagcagg cactctgccc
     4681 agtatcatta caataatcat ataccgtgct gtaattatct gcaagctgtg ccccgacatt
     4741 aaacaattgt tcgtcaaaac aggtcggcgt gtcatatcca tgcaaataag atactgcatt
     4801 ccacatctct gccgcatgtt tattgagatt tcggttattt tccgtcagat ttgccccaaa
     4861 tctttcgtag gcatccgaat gatactttgt ataataaatc acaccatata aattatcatc
     4921 ataatctatg gcnnnnnnnn nncttcataa cccaggcgta cataatggtc taccttatct
     4981 ccataattat atacattcgt ctcaaatccc gggctgacac ttccatagca gacgatcaca
     5041 ccatccgcat ctgtctccag atacagatag tcactgtact tatccccgta aaacgtataa
     5101 gcatatccgc cccagtaaga tggtgttgta agctttggtt ttccaaacag cttttctacc
     5161 tctgcgatct tcatgccact gtttactgtt tttcctccta ctgtgatcag tctgccttct
     5221 atatttgctg cagcacttac agtcacagac attgcaggaa caaacgtcat aatcagaagc
     5281 agcactgcca taacaagaca tcccgttttc tttcgcaaat ttttcatttt gctctcctcc
     5341 ttttgccaaa catccgctat tgtgaagctt tttaaaaatt atagcatctc ccgattattt
     5401 cttcaacaga caggtttgca aaataccatg aaatagtttc tccgaaagct ctttatatca
     5461 tgtctgtgct cctgaccgcc cgcagcgaat cttcatatat cttttgtact ttcaggaatg
     5521 ctttatgcac gctgcagtag tctgtggcat tcctgaaaca aaatccatct tcttccaggc
     5581 atcggtttat tttcatcgtg acttctgttg atgatatgat atcaaacaga gaaatgtttt
     5641 ccggattttc agaaagcaga tatccgcctt tgatcccttc acaggctgtg atgatatttg
     5701 cgtcttttaa tctttttgtg atctttggaa tataactttc cggtattgtg aactacccat
     5761 tgtctaaagc caatgggctt cctgcttcgc agacctcg
//
LOCUS       DAWDWH010000130         5735 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_5998_length_5735_cov_7.654401, whole genome shotgun sequence.
ACCESSION   DAWDWH010000130 DAWDWH010000000
VERSION     DAWDWH010000130.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5998_length_5735_cov_7.654401"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1289..1298
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2715..2724
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3793..3802
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1293 a         1409 c         1101 g         1902 t
ORIGIN      
        1 tctgcttttt cagctgtttc agctttggca gcctttgcag cttttgttgt ttcttttact
       61 tctgcaactt tttcttttac tgcttctttt acttctgctg ctttttctgc aactttttct
      121 actgctgtaa ctttttctgc tgtttcttca gcaacagttg tctttacttc tttctttgct
      181 gcttctttgt ttacagcagc tgctgcattt ttctttgcct gtctacggtt tgccataata
      241 gtttcctcct cataaaaaaa cattctaaac tctcttgttt ttaatgcttc ttaagcaatc
      301 ggcttaatca tataccattt tttacaaatt atcaactata aattcaaatt gcacagatcc
      361 ttgtacatat atttttcgag attttgtctt aaaatggcat aatctttccc ttccggatag
      421 gatggatcct gcagggtgtc gtttgctgca tcgtttgctt cctgtaagat cgcggcatcc
      481 tgaaaaacat ctccgatgcg aaatgcaagc tctccgctct ggcggattcc aaagagatct
      541 cccggtcccc tcagacgcag atcttcgttc gcaatataaa aaccgtcgtt ggatttgttc
      601 aaaatttcca gacgcttttt tgtcttttct ccgtctttcc cataacacag gatacaataa
      661 gactgtgcat ctcctcttcc gacacgcccg cgaagctgat gcagctgtgc aagaccgaaa
      721 cgctccgcat tttcgatcat cataacgctg gcgttcggta cattcacacc aacttcgatc
      781 accgtcgtag aaacaagaac ctggatttca tttttcacaa aagcttccat gatctcgttc
      841 ttcttggccg gtttcatctt gccatggaga tacgaaaccg taacatcctg tggcagtgta
      901 cttctcagtt tttctgtata agaaatcaca tcttctgctt cgatctgctc actctcctcc
      961 accatcggac agatgatata cgcctgatgc cccatcttta cctgtttttc tataaaacga
     1021 tatgctttcg gacggtaaga ggtgtcaacc acacagtttt tgaccggcag acggtttgcc
     1081 ggaagttcat tcacaaccga aatatcaaga tctccataca ggatcacggc cagtgtcctt
     1141 gggatcggag ttgcactcat aaccatcaca tgcggcatat ctcctttttc cgacagagac
     1201 tctctttgcc gcacaccgaa acggtgctgc tcatcggtaa tcacaagtgc cagattttta
     1261 tattggatct tttcctgaaa gaccgcatnn nnnnnnnntt tttgtttcgc tgtcatggaa
     1321 ccggtaagca gttctgcata aaaaggcagt ttctgttcgc agatctgctt ctggatagcc
     1381 tccatatgct gcactgcaag cacctcggtc ggaaccatca ggcatccctg ccagccattt
     1441 cccgctgcaa gaaatagggc aagaaaggca aggatcgtct ttccagatcc tacatctccc
     1501 tgtatcaggc gtgacatcag ataatcacct gtcatatcac gctcgatctc ctgccagacc
     1561 tttttttgtg ccccggtaag tgaaaaagat aagtttgcaa taatctgatc tgtcagagga
     1621 attttctgta tctgaaatct ctgcggattt cgttcattta cctgcttcag ctgccgcagt
     1681 gcaaggataa acagataaaa ttcatcaaat accagtcttc ttctcgccag aagcatctgt
     1741 tcccgattct ccggaaaatg aatctgttca agtgcaaaat gatattctgc gagatgatac
     1801 ttgctgcgga tagcaggcgg cagtgtctct ttgaaaaagg gcagactttc aagtgcctgc
     1861 cgcactgctt tctcgatggt ctttgcagag agtccttccg tcagaggata caccggtttt
     1921 aaatgctgcc gcatcctgtg atattcctcg aaagaaaaaa cagctggctg ctccaatacc
     1981 aactgtctgt ttttctttgc tgctttgccc cacagcacat aaggcacacc ggctcttacg
     2041 gtgctgcgaa gataaggcat attataccag gtgatgcgta ttttctgtgc atcactttcg
     2101 aacacgccgg atataatctg catgtttttt acacgcttca ctgccagcgg tccctgaaac
     2161 tgaccataca gagcagccgt catgccgtct gttacggcat ccggcttctg tatttccccg
     2221 tacgtttcat acgttctcgg atagtaggaa agcagttctc cgacattcgt tacgcccact
     2281 tttgcaaaca gcttttctgt cttttcacca attcctttta gctctgtaag cggtgataat
     2341 gtattcataa ttactccaca gaaacaacat aataatagat cggctgacct ccggcataga
     2401 cttccacatc acaggaagga tatttttccg tcagtgcctg ttccagtttt tctgcatctt
     2461 cggcttttgt atcctctccg taatataccg tgatcagttc tgcatcatcg gcatccatca
     2521 gggcaatcgt ttccagtgta acatcctgaa tctcttttcc gaccgcaagg atttcatgat
     2581 cgccgattcc catgatatcg ccctcatgga tctccttgtc atcaatgtgc gtgtcacgca
     2641 cggcataagt gatctgtccg gtttttacat ttttgatctc ttcagacatt gcctccgtat
     2701 tttcttccac agaannnnnn nnnnatcttc ggtactcgga ttcatggtct gaccgccttc
     2761 gatcagataa tcaacgccca gttcgcggaa gatttcacca ataccctcac caatggaaac
     2821 ggaaataaat ccgaccggct tcggcggcac cattttttta cgctcttctt cttcgaactt
     2881 ctgctgttct gcaacttttg ccgcatcttt gatcagtttc tcctgatgct cctcacgcat
     2941 attatcaatc ttgatacggg tcagtgagcc gtaagtgagt gctctggaga ttgctttgcc
     3001 agggtcgttt gtatgtacat ggaccttgac aacatcttca tccgacacaa caacgatgga
     3061 atcaccgatg gactccagaa atcctttgaa ctcctgctcc gtctgcggtg tatactcttt
     3121 ttcgagcatg ataataaatt cggtacagta accgaatcga atctctgcct gagccggtgc
     3181 ttccttttcc tgtgccgggg cttttttagt gctttcagcc ggttcaaccg tataatcgat
     3241 ctcttttcca agaaatgcat cgtaagcacc ctttaacacc tgcacaagtc cctgtccgcc
     3301 ggagtctaca acgcctgctt ctttcagaac cggcaacatc tccggtgtct ttgcaaggac
     3361 agcatctgcc tcttcgatca cctgttttaa gaaatcatcc agcgaaatat cattctctgc
     3421 aagctccagt gctttgtctg ctgcgccctt tgccactgtg aggatcgtgc cttcctttgg
     3481 cttcataact gctttgtatg cagtctccac tgctttctga aaagcagctg ccagaatcgc
     3541 aacatcaatc tcctcatact ttttgattcc ttttgtaaat ccacggaaca gctgagagag
     3601 gataacacct gagttaccgc gtgctccgcg aagagatccg gaagaaatcg cttttgcaag
     3661 tacttccatc gtcggatctt ccagtgccgc tacttctttg gccgcagcca tgatggtcat
     3721 ggtcatgttg gttccggtat caccgtccgg taccgggaaa acattcagtt cattgatcca
     3781 ttctttcttt gannnnnnnn nntgacattg ttgatcagat tgtctgcaac cgcagaaatg
     3841 ctgacgccat acgccacaat cacatgaaaa tcaagtgaaa tttcattatc ttcaacggtt
     3901 acctgtattc catttttcag gctgtctttc ttcagaagtc ttaccagacc gtctttcatg
     3961 ctcaccgttg ccataccgac aattccaaaa cactctactg caacacttcc ggcataggta
     4021 gcgatcacat ccgtgtcgat caaaatcgaa cccagttctg tttccattct tcctttcata
     4081 tcatgatacc tcctaggtta atagctgtat tatttattca ttataacctg tttcatataa
     4141 aaagaaaaca tgaaaaatca ttttttacag tttttttaaa ttttcgcatt ttttgcttgc
     4201 attatcatgc agcttctggt agaatactta ttgttgtgag cctattgtag gcaatataat
     4261 gtcaaggagg tgcagtcatg gctagatgtg ctatttgtga taagggagct catttcggta
     4321 acaacgtgag tcattctcat agaagatcca ataaaatgtg gaaatccaac attaaatctg
     4381 ttcgcgttaa agtaaacggt ggaacaaaga aaatgtatgt atgtacttca tgcttacgtt
     4441 ctgataaagt tgagagagca taaggatcat aaatgaaaac agggatataa aagagcaggg
     4501 atcagatgac agatcttccg gctctttttt cttttgctca aattctcatt ctggtcagag
     4561 catcctatct gaatcttata cagcaaaaga gcggggaaac cttttggggg tctctcccgc
     4621 tcttttttct tgctgtcgga tgtttctttg gaacggttct gttacagagt agcttttact
     4681 gttccggaaa catgttccgt ttttttattt ttttacagtc gctttctttg gcagacctgc
     4741 ttttttcagc attctcttat acttggaaag cttcttggcc ggtacggtca cttttgcttt
     4801 tgcatcgatc ttgctgaatg ccttgcttcc gatggaagta atgccatttg cttttttcat
     4861 attgacagtc ttcagtttgc tgcagcctgc aaatgcattc ttgccaatct tcttcacatt
     4921 gcttccgatg gttactttct tcagcttcct gcagttcttg aatgcatttt tgctgatctt
     4981 ggtcactttg taagtaacac cgttgatctt aacggtcttg gcaacgttta cagaagttac
     5041 tttcttatct gctgtaacaa ctgctgctgt cttcttcttt gcattcagga tctgataagt
     5101 gaccttattc agaactttct tgttcacagt cggtttttcc ggctcatctg gtttctctgt
     5161 aggtctctcg gtcggttttt ccgttggttt ttctgttggt ttttcggttg gtttttccgt
     5221 tggtttttcg gtcggttttt ccgtttccga ttctactttc ttcagttttg cttttgcatc
     5281 ctgtaaagcg gtcagcagtt tcttcagctc ctctgctgtt gcatctgctt tggcatttac
     5341 agcgttttca tatgctgttt taaatgctgc ccagctttct ttcgtgtaag taccggatgc
     5401 attaccatct tcgtagatac cctttgtttc tgcattttcc actgcatttc ccagagcggt
     5461 ctttgcctgt tcgatttcct cttttttcag atcttcgctg gtctgtttcg ggcactccag
     5521 tgtgatgatc tcacagatgg acggtgctgt ttcaccccag gtgatcttta catcgtatac
     5581 ctgatcatat ccatacgtat agaaggtgtt caggctctta tccagtttac cgatcgtctc
     5641 ccaggtgtct gcaccggtac ggatggaaac ttctgcattg ctgatggatt tctgtacaat
     5701 gttcagtttg cggacattgg ttgtatcaga cagat
//
LOCUS       DAWDWH010000131         5644 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6086_length_5644_cov_12.044194, whole genome shotgun sequence.
ACCESSION   DAWDWH010000131 DAWDWH010000000
VERSION     DAWDWH010000131.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6086_length_5644_cov_12.044194"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    900..909
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2189..2198
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1713 a         1039 c         1457 g         1415 t
ORIGIN      
        1 atgcttccgg cgtgaaaaag cagaagtatt ttgaggttac aagagcagat atagaaagtc
       61 ttggaagtga aaacggatat ttttccatta cagaaatttc ggtcaatggc aaaacttatg
      121 aggctcctgg aacaaagaat ctaaatggtc tgtttccggt ttatgtggac atggagtatc
      181 agccgggaaa tcagacaggc agcacttcca acggtgtatt ttatgttgga aactacacga
      241 aagatcagga tgttgttctg gagttccatc ataaaaatga gatccggacg gtattagaga
      301 tctcaatccc gaaaggaacc cagaagtatc tgctaacagc agaagatatg aagtctatga
      361 cagaaggaga agaatacagc tactgtatcc gtcaggcggg ggatcgccat gcagtcctgc
      421 ttcgaagcaa gcagagagtc gaggaaggtt ttgaagcggc aggtatttct atgacgggat
      481 atccggcgac accatcaacg actgtaacgc tggaatatgg tgacagcact gcgaaattgt
      541 tctattgtgt gatgacagag cagaatttcc agagtaaata tgattatcag acagaactgg
      601 taccggataa tcagtggatc gcctatgcag gtgcctttac gccgttcgca ggaatgacaa
      661 aatccggtac gatgatgatt ctgacaaaag tagtcaatgc agccggaaca aaagcaaaat
      721 atgcatatta tgatgtggaa gttcgggcag gtgcaggaaa tgatcaggat gatgatcata
      781 aagacgataa cggagataag ggaaacagca acggcacaaa tggtggaaac cagggcaaca
      841 acgggaacca gaatacaaac aatggaaacc agaacacgga cggaactctt gcagtcggtn
      901 nnnnnnnnnc gcatctacag ttgcatttgc cggagtgatc aacagcaaga cgaaaaaagt
      961 caatattcca aagacggttc agatcaataa gaaaacttac aaagttgtac agatcgcaaa
     1021 aaatgccctc aaaggaagcg atgtacagac tgttgtgatc ggagcgaacg ttaccgcaat
     1081 ccaggatcag gcatttgccg gatgcaagaa actgaaaaag gtaaccgtcg gagcaaatgt
     1141 gaaaagtatc ggaaaagcag cgtttaagga ctgcaaaaac ctgaaaaaca ttaccgtaaa
     1201 aagcacgaaa ctgaagaccg tcaaagcaaa ggcactcaag ggcattaaca aaaaagcgac
     1261 gatcaaggtt ccggcaaaga aactcaaaaa ttaccagaaa ctgttccgca aaaaaggaca
     1321 gagcagttcg gtaaaaatta aaaaataata acaggatcgt aacggttttg cacagggcga
     1381 agccgttacg gaggatttta tatggcacag caaaattcaa agaaaaagac aaaaaatcaa
     1441 atacaatgga agagcagtcc atataaaaga acgaaagaag aggcagctgt ttttacagtg
     1501 cttcttcttt ctgcattttt gtatggagga ttttatgaat tttccggagc gatgcttgcg
     1561 gcagtgcttg gtatactgct tatcgtctgc cgcgtaaaat attcgtttca gatcaaaggg
     1621 aaaaaacagc tggcagtgtt gctgtctgtg tttttatggt tcggtattac ctgtttttat
     1681 gcaatcgatc gggcaatggc ttttgtgggc gtgatcaaaa tgctgccact gcctcttggc
     1741 tatctgtttt tcatgcagtt ttcggatgaa accagacaga aagcaacagg ctatatcgca
     1801 cacatcggct gctttatggt actggccggg attctggcac tgccatttcc ggctctgaaa
     1861 gagcaactct ggcaggccgg gcgtcttggc ggatttttcc agtattctaa tacctgtgca
     1921 ctctatctgc tggcaggatt ggttgtgatc tgcaatcgat ggatcgaaaa caaaagcaga
     1981 gaacaggaat cgggcatcaa aagagacaaa atgcagattc tggaaggaat tgtacttttg
     2041 gcaggattgc ttctgactgg gagccgcggc gtgatgcttc tgtttttcgg atatctgatc
     2101 tggtttatcc ggcatctgtc gtcacaaaag gcaaagaaat acgggatttt ttgcattgtc
     2161 gctgtgtttg cactacttgc agttgttann nnnnnnnnca gaatatcggc aggattttca
     2221 cagtgttccg ttattccagt acctggaaag gacgtattct ttatgatctg gatgcattaa
     2281 aaatgatcgc aaagtatccg tttggaatgg gttaccatgg ctatgcttat gttcaaggac
     2341 ggatgcagac cggagtttat aagacgctct ttgtgcataa cgactggtta caggctgcgt
     2401 tggatctggg tattcttccg gctgttctgt ttgcggctgt gatgctgcgt cagctcttaa
     2461 aaggcagtca gagcagtatg cagaaacaga ttttgcttct tatcatgctg catatgctgg
     2521 ttgattttga tctgcagttt actgcgatcg ggcttttggg acttctgtgt ctggattacg
     2581 gtaaagcaga gggtagttta aagaaaaaga caaaaatcga agactgtatt tttctgacag
     2641 ttataagtgt gggctgtatt tatttctgca taccgttcct gctcgattat gtcggagaac
     2701 atcagaaggc actgagtttt tatggtgctt atacgccggc acagcttgcg gtgatggaag
     2761 gaagtcggag tgcagagaaa gcaacttcgg cggcagagca gatcctcacg cacaatacac
     2821 atctggcaga agtttatcaa tatctggcat atggtgctct ggaaaaagaa aattatgaag
     2881 aaagctttat caatatggaa aaagccattt cctgtgatat ttatgcgaaa gaattatatg
     2941 acgagtacga agaaatctta agtcaggcag aaaaagtgca gggcagtgat cagagtgaac
     3001 ggcgtgcctg gatccagaat aaaaagcagg aagtgttaaa gaagagcagc agtctcgcat
     3061 atgaattaaa tgacagacct cagttataaa ttcaagttgc cctggctgtc aaagaggact
     3121 ataatatctg ataagaaaag atactatggt cggaagaatt ccggcttctg gtatctgaga
     3181 aaggatcagg aggcagaatc atgcgtgcat atgaaagatt attggaatat gtcaaagtaa
     3241 aaacaccgag cagcgaagaa agtatggaaa caccgagcag ttcctgtcag tttgaactgg
     3301 cagagcgtct ggtgggagaa atgaaggaac tgggcatcga ggatgcagcc gttgacggaa
     3361 aatgctatgt ctatggaaca attccggcaa cggaaggtta tgagaacagt ccatctcttg
     3421 gatttatcgc acatatggac accgtttcag aatttacgga aaatccgatc catccggttc
     3481 ttcatgaaaa ttacgacgga cagaatcttg tttttgatga aggcggcaga gttttagata
     3541 ccgtactttt tccacatctg aaaaatctga agggacgtac gctgatcacg tcagacggaa
     3601 cgacgatcct tggagcagat gacaaagcag gtattgcaga aattatgacg atggcagaat
     3661 acgttctgcg aagtgatgtg ccgcatggga agatctgtct tgcatttacg ccagacgaag
     3721 agattggcag aggggcagat cattttgatg tagagggatt tgcagcagat tttgcctata
     3781 ccgtggacgg cggtgcggaa ggcgagatcg aatacgaaaa tttcaatgcc tgcgaagctg
     3841 tttttaagat tcagggggtc aacgtgcatc cgggcagtgc caaaggcatc atggtcaatg
     3901 caggagctgt cggatgtgag atccagatga tgctgccggt ggaagagaca ccggaacaga
     3961 ccgaagggta cgaaggattt tatcatctga tcaaaatcag cggaagctgc gaaagggcag
     4021 aacttatcta cattgtgcgt gaccacgatg cggacaagtt tgcacagaaa cagcagaaac
     4081 tgaaagaaat cgcagatgtc atgaatcaga aatacggtga gggaacggtt gatcttcaga
     4141 tcaaagaaca atatcgaaac atggcagaga agatccgtcc ttgttatcat ctgatcgaga
     4201 atgcgaaaaa agcctgtgag aacgtgggta ttgtgccgaa aattcagccg atccgcggcg
     4261 gtacggatgg ggcaagattg agttttatgg gactgccgtg cccgaatctc ggaaccggcg
     4321 gctatgcatt ccatggaccg tatgaacata taacagttga gggaatggat aagagcgtgg
     4381 aaatcctgat ggaacttgta aaaatatatg gaattattaa aaaagaataa atttttcttg
     4441 cagaggctct tcaggtatgc taaaataaaa acacgttgtg tcgaagaaag tcacagcgta
     4501 ctctggagag tctcatagaa cgggcgccga aggtgtacgg cagcagatct gctgcttatc
     4561 tctcaggcaa aaggacagag aaaagtctaa atatgttcta ctctttggag ggctgattgt
     4621 atgatcagct cttttgttgt tttcggacag cccaaagaat cgatccgtgt cagtatcaga
     4681 caacatggaa aatagtcacg gggcagattt tgtgagggtg ttctggcagc agccaataaa
     4741 taatcaagat tggagagaga gtaatgttcg aagcagtaaa caattttttg cagcacgttg
     4801 atgatctggt atggggtgtc ccactgatcg tagcaattct tttgactggt atttatctta
     4861 ccattcgtct gaaaggtatc caaatcacaa aacttccaaa agcactcaag tacatgtttg
     4921 aaaatgaaga gagcggtcac ggagaagtta caagtttcgg agcgttatgt actgcacttt
     4981 ccgcaaccat cggtaccgga aatatcgttg gtgtggcaac cgcgatcgta gccggaggac
     5041 cgggagcgtt attctggatg gaaattgcag ccctgtttgg tatggcaacg aaatattccg
     5101 aaggtcttct cgctatcaaa taccgtacaa cggacaaaca tggtcatatc cttggcggcc
     5161 cgttttacta tatcgaaaat ggtatgggta aaaaatggac ctggctggca aagatttttg
     5221 catttttcgg aatgggtgta ggtctgtttg gtatcggaac ctttacacag gtaaatggta
     5281 tcacaagtgc cgtaggtaac ttctttgatc cgaacgcaca gaatgtgatc catctgctgg
     5341 gaagagatta tacgcttgcc gttgtgattt caggactgat cctgactgtc tgtgtcggtc
     5401 tggttgttat cggaggattg aaacgtattg caaaagtatc tgagatcatt gtgccgttca
     5461 tggcagttat atacattgca atttctttgc tgcttctgat cttaaacgct aataaaatcc
     5521 cggcagcgat cgtagagatt gtatccagtg cctttggtat gcgtgcagca gccggcggag
     5581 cacttggagc gatcattgtg gcaatgcaga aaggtatcgc aagaggtatc ttctcaaacg
     5641 aggc
//
LOCUS       DAWDWH010000132         5626 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6106_length_5626_cov_9.968946, whole genome shotgun sequence.
ACCESSION   DAWDWH010000132 DAWDWH010000000
VERSION     DAWDWH010000132.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6106_length_5626_cov_9.968946"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3769..3778
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5289..5298
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1327 a         1462 c         1041 g         1776 t
ORIGIN      
        1 tgtcacaatt ctatctcctt attcctaaac ttgcattcta agtggcattt tagcacatat
       61 tgtctttgtc tggcaatctt tttctgctat ctgccggcaa gcaccctgtc caggatatct
      121 gccagcggct ccatggcatt tttctcttct tctacggtgt tcagctggct gcccgcctcg
      181 atcagcagtg cccgcggctt cagatgcaga ttgtagcgaa gactctgaag ataaatatgt
      241 ctggtgaacc ccggataata ttcctgtgct ttgatctgaa gctgaaagga aaacgccaga
      301 ttctcttcaa tatacggatt aggcagatag tcaacgtttc cgtttctcgc agtccggctc
      361 agcccgttaa aaaacatgat cttcgctacc tgtttgccat cgatctgcgt gaccagcttc
      421 tccccatcca gcccatctcg gtgcaggtcg atcaccacct caaccgttgg atttgccgca
      481 agaatgctgc tgatctcagt ctccgccaga ttgtatgccg tgtttcgatc caacacaccg
      541 tctatcatgt caaatacgct tgtattatgg attacattat atccatactt gttttccagc
      601 agctctgtca gataatttcc catgcccaca atcgtatcag aaatatttcc ttcttccgaa
      661 tctgcaaatg cttcctgcga atgggtatga tagatcagaa tctgcggcac agatgcatct
      721 ttttttatgc gaagatcctg acctgcaagt ttctctgcat tgatctgctc cggatccgct
      781 gttgtagatg catctacggt ataataattt ccgaccagcg tctggaaatc ctgaagttct
      841 tccatattta ccgtttcctg ctgttctttt gtttcttcgg tctgtgcttt tttgtttttc
      901 tttctctttt tcttttttac tgctttttga atgaccgttt tttctgtctc ctccatggat
      961 tctgcttctg ccttctgcgg catctgatcc tgtgtgttct gtacttctga agacttttgc
     1021 gtttcctgtg ttttttcact ttccggattt ttatctgttt cttcccgtac cgtcagctgc
     1081 ggctcttctg ataagaaata ggactccttg ctctcccagt aagtcagaag cggaacgact
     1141 tcttttgtaa gcagggaaag ccagctttga ttctctgtac ctttcactgg tgcaagtgta
     1201 aataccggtg catagagctg tgctgttttc cttgtcaaat ttttgccccc gcctgcgatc
     1261 gacacaccct tcaccagaac ataaagcccc aggccgaaaa tgactgccca cagacttccg
     1321 tgtatccagt ttttaaaacg cttcatgccc tcacctcatt cattgcaata tatgaggtgt
     1381 tttttttcat tatgcaagct gaagggcaag attgagccct tctgaaatcg tatagctcag
     1441 acgtttcacc gtctcatcaa tatccttggg cgtcacaaac atcgtattta aatgtgatgg
     1501 aatcctttct ttgatcagct gatatttctg tgcataatct tccatatcaa gggcatgaat
     1561 caggctctcc atcgtgggca cgccgatggc aatgaccgga atgccaagaa cctgtttatt
     1621 taaaccattt ctgtgatttc cgacaccgga acccggattg atcccggtat ctgtgatctg
     1681 gatcgtacga ttcagcctgc gtgtactgcg ggctgccaga gcatccactg caatgatcag
     1741 gtcgggatgc gtttccttta ccacgcctct gatgatttcc agcgtctcca tgcctgtctg
     1801 agccatcact cccggaacga tgccgctgac cggatttcgc tgttcttccc caagcgaagc
     1861 tgccccgaat tcccggatca tatgcctggt gatgcagatg ttgccaacca cataaggtcc
     1921 gagtgcatcc ggtgtcacat cccggtttcc aagtccgacg atcagcaccg atttttcccc
     1981 ttctcccatc agacttttca gatgttctgc cagtttctgc gatatttccc ggtgataatc
     2041 ctcatccggt gccgacatat tcggggcttc catggtaata tatgtcccga ccggtttccc
     2101 catcgttttt gccccgtttt ctgtctcgat caccaccctg gtcgtatgga tctccagttc
     2161 ttcatcgtcc tcctcttcaa tccgcacacc ccggattttt acgtcatctt cttcaaaact
     2221 ttctcttgct tccagtgcga ggtctgttct gatacgaaat cttccaaaca tgctctgttc
     2281 cctcctgctg tttcgttttt ttgcctgatt ttgtttccat ctgatctgct tttatcagtg
     2341 tctgtaattc ttatcgaaat attccatatt ttttcaaaat ttgatattga catttggcag
     2401 gctttggttt agactatgag agtatgtttg aagtaaaatt agaataaaaa attggaggtg
     2461 tacagattgg ctaacatcaa atccgcaaag aaaagaatcc tggttaacag aacaaaagct
     2521 gagcgtaata aatctatcag atctgctgtt aaaactgcta tcaaaaaagt agaggttgca
     2581 gttgttaaca aagataaaga aactgcagta aatgcactgg gtgctgctac agctatcatc
     2641 aacaaagctg catcgaaagg tgtttatcac aaaaacaacg ctgctagaaa agtttctcgt
     2701 ttagcaaaag ctgtaaacag cttaaattaa accaattttt ataaatatat aacccatcag
     2761 gtaccgtcag cctgatgggt tttttatttt gaaggcaaaa acactcttat caactcgaat
     2821 atttgacaag cagaagctcc acgctcatca catcgttcat acgccctgtt ttgactgcct
     2881 cttccatcct gacgcagtcc tgcagagctt cttttaaggt tttcagtgaa aagtattctg
     2941 cctgtcgcag acagttccgc acaataaatc ctgccatttt cagacgctcc gaaatcgttt
     3001 ttgcactgta tccttcctcg gaaagctctt ttacctgcat cagaagatta aactggcgtg
     3061 tgatcagata gagaatacgc atcggcggtt cttttaatgc cagcagatca tagtaaagct
     3121 ccatagcttt cttctgcttt ttctctgcca tcgcattgat catatcgaaa attttatttt
     3181 ccgtttgctc actgcagatc actgcgacat cttcttcggt aatggattct ttctccatac
     3241 agtaacagat cagtttttcc agttcctgac gtatccgctc catatcggtt cccgttttct
     3301 ccagaaacag tttcatcgca ccggaagtga tcatcttttt ttctgatttc agtatgccaa
     3361 ggatccacct ggtcagcgtc tgttcattct gtgccgtaaa ctcaacgaca ctgccttttt
     3421 tatttaccgc cttataaagt ttgcctcttt tgtctacttc gctttctaca aacagcatga
     3481 tcgtcccttc cggtatggat ggaatataat ctgccagctg ctgatttgca tttttaaaaa
     3541 agccgctgtc ctcgatcaga agcaggcgat gttctgcaaa gaacggcatg gtatccgcct
     3601 gtgcgatcat ttcctctaca tcgatgcctt ttccactgta aatgctcaga ttgatggtat
     3661 ccccttccgg aagaactgca tctttcagac gatttttgta ttgctgtttc agataggctt
     3721 cttccccata gaagagatat accggcttaa agcttccttt tttgatatnn nnnnnnnntt
     3781 tccttcgcag tcagagcttt ccagccctgc tccataactt tatcgagaag tccgagctct
     3841 tttgcgatct catactttaa ttcttcttcc ggtgccagat tatttaaatc aattttttct
     3901 tttttcttag acatatagac catacccctt tcttttctgg agtatggcct gatttttcat
     3961 ttactattca tatggacatg aagtttgagg caaaaagtct tttgacccaa attttaacct
     4021 tttaaccctg acatcatgct atggtttcag tactcttccg gcatgataat cgctgcaatg
     4081 atatacagga tcagtccagt actggtgcag ccaagcagca cacataacag acgcacgatc
     4141 gtaggatcaa tgccaaaata ttcgccgatt ccgccgcaca cgccgcataa cactctgttt
     4201 gttcttgatt tgtaaagtct ctttggttcc ataatctttc atcctttcat aattaattat
     4261 gatatgttta tcctatgttt ctgtaactga tttgtacctt tgtcggcttt cgtattattc
     4321 caccgatatc tcagacaacg ttcgaaatat cttccagaat tttttccgga atatcctgca
     4381 cgctcggaaa ttcctgcatt tcttcataga gcctgccgct gcctgctccc actgcaaatg
     4441 ctgccgtaca aagtccgatg ccgcacacca gcaggagcac tgctgcggta agtgcgattt
     4501 tcgtccgttt tttcatgcca tcctgctcct tcccgaaaaa attttatgaa gtgcttttcc
     4561 tgccatccgg atcagaaccg gaagcatttt cagataaagc catatgcaca gaaatgtcat
     4621 aaaaatccca acggcaaggc cgatacatcc aaggccgagg gacatcagac cgcgaaatgg
     4681 gtttacaaac agcatcacgg ctccgtaaac aatactgact gctccaaata tataagccgc
     4741 gatcataatg ccaagtgctc cggccagaat cccaatgatc agtccgaata tgccaaccac
     4801 gatcccgatc agtcctccaa gcagaccggt tgctgttcca agaacggctc ctgcagccgg
     4861 gatcagaatc gccagtgcca gtgcaaacca aatcaccctc atgatcgtgt tctgatcttc
     4921 ccaccagctc cagaatgacc actttgcagc agatgctttt gctctgtcgc ctttcttttc
     4981 ctcctccaca taaaaagccg gtgtcttgct ttctgtatcg gatgctgcac tttctgcacc
     5041 gttttctgta taaaagtact ccccttttaa tccacttttg atatcctgtg ccaccttctg
     5101 cgggcttccc agatggtcga ttgcctgctg caggtcatct cctgcttcat caaaataccg
     5161 ctcgtaataa tccagtgctt cctcccgctc ctgcggtgaa atatcaccaa gaagtgccgc
     5221 aagttgttcc ataaactctt tcctgcccac ttaaagttca cctccgtcaa aaatattgcc
     5281 aatcctggnn nnnnnnnnct ttccactctg tcttatacag ctgaagctgg gccatcccgc
     5341 gttctgtaat tttataataa cgccggtttc gaccgccaaa ttccatatca tagacttcaa
     5401 gacagtcatc cttcaaaagc ctgcgaagca ccggatacag cgtcgattct gagacatcga
     5461 tcgcttttcg gacatcctgg gtgatgcggt atccgtaagt acccgcttcc tctttcgaca
     5521 caacggccag tacaatggca tccagaagtg ccgcccctgt attaaaaacc atagcttcct
     5581 cctgatacta ttttcttttc aatactatat atcatataat attgtt
//
LOCUS       DAWDWH010000133         5613 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6119_length_5613_cov_11.281756, whole genome shotgun sequence.
ACCESSION   DAWDWH010000133 DAWDWH010000000
VERSION     DAWDWH010000133.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6119_length_5613_cov_11.281756"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1475 a         1345 c         1172 g         1621 t
ORIGIN      
        1 atgcaggggc tttctttatg cacttgggag aaatattatg gtaagaagaa aaaccgcttt
       61 aaggggcgta tctgcgtcca ctcgcataac taaagtagca atatacatca gagtttctac
      121 catacaccag gtagacaaag attccatacc tatgcagaag aaggacctca tagcgtattg
      181 tcagcttatt cttggcactg ataattatga gatttttgaa gatgctggtt actcaggaaa
      241 aaacactgat cgtccagctt tccaagatat gatgggaagg atccggaagg gtgagttcac
      301 tcatgttctt gtttggaaaa ttgatagaat atccagaaat cttctggact ttgcagaaat
      361 gtacgaagaa cttcaatcac tgcgtgttac cttcgtaagt aaaaatgagc agtttgattc
      421 ctccactgct attggcgaag ctatgttaaa aattgttttg gtatttgctg agttggaaag
      481 aaacatgacc tcggagcgtg tcacagcaac gatgatatcc agagccaaca atgggcaatg
      541 gaacgggggg aggattcctt ttggttattc ctacgattct ggaacatcta ctttctctat
      601 cagggaagac gaggccgtag tatgccgcag gctaaaagac ctatatctgg acaataaatc
      661 cctcgtctat actgcaaggg ctctgaatgc agacggttat aaaaccaggg caggtgcgga
      721 ctggacacct actgcggtat ggattattgc atccagtcca ttttacgccg gcatttatcg
      781 ctataaccgg tataagggaa cagagaacag aactctcaat ccagaggaag aatgggtaat
      841 gattccagag catcatcctg ctatttttac tttggaagaa cacgaagcaa tgaagtcctc
      901 attaaaaaca aatgccagat acgttgataa tccagttgga aaaccgcacg ctactgccaa
      961 tatccatata tttcagggaa ttgcctattg tggcaaatgc ggaaacaaaa tggtttccac
     1021 tcccggaaag aaacacgctg acgggtaccg gccatcaaat tatagttgcc ctctgcatcg
     1081 tagaagtaaa aaatgcgaca atgccactgt gaatgatatg atcgttggag aatttgccat
     1141 caattatatt ttgaatatgc tcaatgcaaa gaaaacattt tccaatatcg acactcccga
     1201 agaattgcag cggcatttgc tgaccggttc cacattttct gatatttcct ccattgagga
     1261 agatgggcta aatgatttct tcaatctttt aaccaggtat ggttcagata attcttatgt
     1321 ttttgcaata aaaactcccc gaaggaaaaa agcgtctgtt aatcctgaac ttgctgctct
     1381 ccggaaggaa aaagaaaagc aggaacgtgc tttacagcga ttgcaagact tatatttgta
     1441 ttcagacaaa tctatgtcgg aaaaagactt cattctccga aaatctgaaa tatctgatcg
     1501 tttaaaagac attaatgctc agcttggcct tattactcaa aatccggatt ctctgctatc
     1561 cgatgaggag tttataaaac aggccagtca tcttctcata cagaaagaat taaaaggcag
     1621 aaaatatatc tattacaaaa atcttgcaac ctcagtggat ccggaaattc tcaggacgta
     1681 catggaaacg attcttgatt ccatgtatat tattgatggt cgtataagtt ccattgtctt
     1741 taaaaatggt cttacacaca aattcatcta taaaaattga caccgacata aatgtcgggc
     1801 acaaaaataa ccaggaagta ttcgtacctc ctggttattc ttatattttc tgtgtttttt
     1861 ctccatccaa caaatgttag aaatggatgt taaatgataa gcatggcatc cccaaaggag
     1921 aagaaacgat aacgctcttt taccgcttcc tcgtaagcag ccatgatctt atcttttccg
     1981 gccagtgcgg aaaccagcat catcagtgta gactccggca ggtggaagtt tgtgatcaga
     2041 cggtcgatca ttttaaacgg ataaccagga taaataaaga tgtccgtcca tccgcttcct
     2101 gcttttaaaa taccgttttc atcggttgcc gactccagcg tacggcagct tgtcgttccg
     2161 actgccacaa tcttcccgcc ttcttttttc acacggttga tcagctccgc ctgctccggc
     2221 tccacaatgt aaaactccga gtgcatgtga tgatctgtca catcttctac cttgaccgga
     2281 cgaaaggttc caagtccgac atgtaaagtc acatgtgcaa tgtgaacgcc tttctttttg
     2341 atttcttcca acagttctgg tgtaaaatgc agtccggctg tcggtgctgc ggcagatcca
     2401 tcgtgctttg catacaccgt ctgataacgg tttttgtcct tgagctggtg atggatgtac
     2461 ggcggcagcg gcatctgtcc cagtttatcc aggatttcct caaaaatccc ctcatactca
     2521 aaacggatca gacggtttcc ttcgtcaacc acatcgatca cctcaccgat cagcagccct
     2581 tcgccaaaac tgattcttgc accgacacga catttcttcc ccggttttac cagcgtctcc
     2641 cagacattat tctctcttct ttttagaagc agtacttcaa tctttgcatc cgttccgatc
     2701 ttgcttccat agagtcttgc cgggattact ttcgtattgt tgactaccag acagtctccc
     2761 gggttcagat attcaatgat atcacggaaa acgcgatgct gcgtctttcc gctttctctg
     2821 tcaagtacca gcagtctgga agaggaacgg tcttccagtg gatcctgtgc gatcagttcc
     2881 tgcggcagat caaaataaaa atccgatgtt ttcatatata tactccttaa taataaatat
     2941 gattttcttc aactaaaacc ggcttctcgc ctctgttaca ggttacaatc gacacggcac
     3001 agttttttga tacgccgcca tgccagtaat cttcccggtt gctttctctt atgctgttga
     3061 gcagtccgcg aagtgcacag ccatgcgtcg acacgaggat cgtcttatct tgaagttctt
     3121 ttcgactgca gatatcttcc ataaaatccc gggtacgctt ttccacagca tccagacttt
     3181 ccccgcccgg tgctgccaca taatcctgcg gggcatggaa gaagttttca atccactgct
     3241 gcggcacctg tcggttttct tctgcataac agcagccctc atagattcca aagctgatct
     3301 cgatcagacg ctcatcctct atgatcggga cctctcttcc ggcaaggata cactgtgccg
     3361 tgtgttttgc acggatcagt ggactggtaa aagccatgtc aaacgttaca tcacgaagtg
     3421 cctttccggt caactctgcc agatagatcc cattttcatt taaatcaacg tcagaacgcc
     3481 cctggaggca cttacgtgca ttccaggggg tctgaccatg acgtatgata tacattttca
     3541 tgtgttattc tccagattac tgacgaagtt cgatacccat gctttccagt ccattgagga
     3601 tcttcttcat caccgtagtg atctctgctt cttccatggt cttgtctttt gcacggaata
     3661 ccacagaata agccatagat ttatagcctt ctttgatctg agttccttca taaatatcaa
     3721 acagtttgaa gctttccaga tgtttgcctc cacgctgaac gatcatctct tcgatttcac
     3781 cggcaagtac atgtttcgga accaccatac tgaggtcacg tgtcacagcc gggaatttcg
     3841 gaataccttc gtatttgcgg tcgaaggaag ccctctccac gatcttcggc atgtccagaa
     3901 cggcaacata tgccttttca ccgattccgt aattatccag aacctgtgga tgaacttcgc
     3961 ccaggaatcc aatcacttct ccgtcgtaga ggatgtttgc ctgtctgccc ggatgtaaag
     4021 atggctgtcc tgcgttcgga tcgtactgcg gaatcttatg catgcctgct ttttccagga
     4081 actcttctac gacacctttc atgacaaaga aatctccgct gccgtacata ccaagcgtga
     4141 actgcatacg ctcatccgga agctcggtaa gcggcagtgc tttcggcaga taaatgtttg
     4201 ccagttcata gagacgaacg tctttgtttc tgtggctgta gttatagcca agagaagaga
     4261 gcataccatt taaggaagtc gttctcatga tgctgaaatc atcgccgagc gggttggaga
     4321 tcctgatggc ctgacgcagt acagaatctt cagggatcag cagtttgtcg aataccttcg
     4381 ggctttcgaa ggaataattc atgccctgag agaatccgca gaattctgcg atatcttttg
     4441 caacattttc tactctgtga tggaactgaa gtttacctgt agtagcttct ccttttggca
     4501 gcgtcgtcgg gatattatca tatccataga aacgtgccac ttcttctgcg atatctgcgg
     4561 tgcggaaaat gtcatgacgg aaggtcggtg caatgatttc attgctctct tcgtcatagg
     4621 caagttctac atttgcaaga taatccaaca tctgttcgct ggacagattg gttccaagca
     4681 gtgcattgat gcgttccggc tcaaatttca cgcgaaccgg ctctttgcgt ttggagtata
     4741 catctgccat gccgccgact acttcaccgg cacccagttc ttcgatcagc tggcatgcac
     4801 gattgatcgc tgcctgtgca ttgttcggat ccagaccttt ttcgaacttg ccggaagcat
     4861 cggttctaag accgattttc tttgcagaaa ggcggatgtt ggtgccatca aaacatgcag
     4921 cctcaaacat catcgtcttt acattgtcag tgatcatgga attctcaccg cccatgatgc
     4981 cggcaatgcc aacagctttc tcaccgtcac agatcatcag aacagaatca tccatcgtac
     5041 gttcctgacc gtccagtgtg gtaaattttt cgccgttttc ggctctgcga accacaatct
     5101 tatgacctgc aagggtgtca taatcatagg catgcatcgg ctggccgtat tcttccatta
     5161 catagtttgt gatatcgaca atgttgttga tcggacggat tccgtttgat gccagtcttc
     5221 tctgcatcca tttcggtgac ggagcgatct tgatattttt tacgacacgt gcacagtatc
     5281 tcgggcagag atccttgtct gccacttcaa cggtcacata atcgtttgca ttttcatcat
     5341 ttccggtctc ttttacaaca ggaggcacaa atttttttcc gaaagttgct gccacttctc
     5401 ttgcaatacc gatcacgctg tagcagtcta cacggttgga tgtgatttcg tattcaaata
     5461 caacgtcatc cagtcccagt gctttgatgg cactttcgcc taccactgca tcttccggga
     5521 agatataaat gccgtattcc ggtgcttccg gatacatatc tctggtagaa ccaagttctt
     5581 cgatggagca catcataccg ttagattcaa ccc
//
LOCUS       DAWDWH010000134         5605 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6131_length_5605_cov_9.810991, whole genome shotgun sequence.
ACCESSION   DAWDWH010000134 DAWDWH010000000
VERSION     DAWDWH010000134.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5605)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6131_length_5605_cov_9.810991"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3061..3070
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3803..3812
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4707..4716
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1260 a         1511 c         1128 g         1676 t
ORIGIN      
        1 gtatgcctga gcacgtactg ctcccggatc gctgtcgatc ttatcgaggt cttcttccat
       61 cggcatggta aacggatgat gcatggcaac gaaacgattc tgttcttcgg accactcaag
      121 cagtgggaac tccgtaaccc acaggaaacg gaaatcgtct tttttcagca gatccagact
      181 ctttgccaga tgcagacgca gtgcaccgag cacatcccaa acaacttttt tcttgtccgc
      241 agcaaacagc agcaagtctc ccggttctcc gtccattgct tttaccagtt cttttaactg
      301 ttcttcttca aagaattttg caaaagaaga tttgtaagtt ccgtcttcat tgactgccag
      361 atatgccagt cctttggcac cataagtctt tgctacttct accagtgcat caatcttctt
      421 tctcggcata tgcccctgtc ctttggcatt gataccgcga acggttcctc cgttttccag
      481 tgctccggta aatacaccga atccgcagcc ttttacgact tccgatacgt cctgcagttc
      541 catgccgaaa cggatatccg gtttgtcaga gccgaaacgg tccatggctt cctgccaggt
      601 catacgctgg atcggcagct gtacatccac gccgatctct ttaaaaatgt atttcaggag
      661 acgctcattg acatcaatga catcgtccat atcaacgaaa gaaagctcca tatcgacctg
      721 tgtgaactcc ggctgacggt ctgctcttaa gtcctcgtca cggaaacatt ttgcgatctg
      781 gaaatagcgg tcgtatccgg aacacataag cagctgtttg agcagctgcg gtgactgcgg
      841 cagtgcatag aagctgcccg gatgtacacg gcttggtacg aggtagtctc ttgcaccttc
      901 cggagtactc ttgatcagga tcggtgtttc gatctccaga aatccttctg catccataaa
      961 gcgacggatc gctgttgcta ctttgcttcg aagcagcagg tttctctgca ggttcggacg
     1021 acgcagatcc agataacgat atttcagacg aagttcatct cttgtctgga tatgctcttc
     1081 gatcggaaac ggcggtgtct cggactcgga aagaatacgc agactctctg ctcttacttc
     1141 cagctcgccg gttgcaagat tatcgttgac tgctccgccg cgtttttcca gtttaccggt
     1201 tacggcaatg acaaactcac ttctcaactt gccggctttt tcatatcctt cggttccgat
     1261 atcattctca tcaaaaacaa tctgtaacag accgcttctg tctcttaaat caataaagat
     1321 cataccacca agatttcttc ttttctgtac ccagcccatg acagttactt cgctgccgat
     1381 cgctgcgttt acgacttctg cacatctgtg gcttctgtgc aggcctttca ttgattcagc
     1441 catttgcttt tgtcctcctt tatttgatgc cgtcacagaa aaattctttg aattctgtgt
     1501 agccttctgc cgtcatctgt tccttctgga attttttatt tttcttcatc atattgacgc
     1561 ttacgatgct gccgttcttg cgttcttctg cggcttcttt gaaaattgca gccagcttgt
     1621 cacccggcat atttttttca atcagatatg ctttcttacc tgcattgctc ggaacctgat
     1681 aatcacgctc cagaagcagc atgacaatac gttcaaatcc gatggagaat ccgcatgcac
     1741 aggtcttctg accggtgaat ttgccgatca tttcatcgta gcggccgccg cctccgacgg
     1801 aacctccgaa tccatcaata ctgatctcga agatcggtcc ggtatagtag gacataccgc
     1861 gaacgagggt cggatcgaac acgatctgga attttgctgt cttgacagtg tcaacactct
     1921 ggatgattgt ctccagttct tctgcgatct tctgatccag gaagccttcc agaagttctt
     1981 ttaagcggcg tacgccattt acatcttcgg tcacctcatc aaacagggac aggtacttgt
     2041 cgatgctctc ttttgcaaat ccttcttttt ccagctcttc tgccacgcct tctttgccga
     2101 tcttgtccat cttatccaga atgataaaga cggtatcgta gctttcttcc gggaaaccgc
     2161 tgcatgctgc catggctttt aagatcctgc ggtcgttgat gcggatcgta aagttttcaa
     2221 agtccagttt gccgaccagc gtggaagttg ccaggatcag ctcgatctct gccaggatcg
     2281 tcggctcgcc gagaatatcg atatcacact gcatgaactg gcggtaacgg cctctctgcg
     2341 gtctgtctgc acgccataca tttcccatct gcagtgcttt gaacggactt ggcagattgt
     2401 ttgcattgtt tgcataatat ctggacagcg gaacagtcag gtcgtatcgc agtccgccgt
     2461 cgaccagatc catctcggtt tcggccgtgt cgatcttcag tttttcgcct ctttttaaaa
     2521 tcttaaagat cagtttttcg ttttctccgc cctgcttgct ggagaggttc tcgatgtgct
     2581 ccacgcaagg tgtctcaatg gaagaaaatc caaacgttcc gtaagtatct ttgatcatac
     2641 ggatcacgta atcgcggatc tccatctcgc gtggtgtaat atctttcatg ccggttaccg
     2701 gcttcttctt cattgccata aagtatcctc ctctaaatcc catttcatct gttcacatgt
     2761 ccggatcctt tcacagatga tctcatcatg aatgatctcc agaaaacaca aaaacagttt
     2821 cagatcaaaa ttttttattt cttcaatcat aatggcaacc gccttttccc atgaaaaagc
     2881 tctgcggtac ggtcgatcgg agatcagtgc cgcaaacaca tcacagatcc gaaggatcct
     2941 cgccccaatg gggatctcat cttccttcag gttcgatggg aaaccgctgc cgtcataatt
     3001 ttcgtgatga tataaaatgc tttttatgat aaaatccgaa taatgatttt gcgatgtgat
     3061 nnnnnnnnnn catatcccag tgtcggatga agccttacat acttgatctc ctcgatcgta
     3121 agcggcttct gtcctttctg atacacatat tttgccatct caagtttccc gatgtcatgc
     3181 agaagtcctg cgatcgacag ctcgtggcac tgttcttctg acagtcccgc ctttcttgca
     3241 agctctccgg ccagaatact gaccatcatc ccatgctgca gttctttgct caggctgaac
     3301 tgtatgatat tatacccggt ttttttcata gtcacaataa ccctgtttct tacgttctat
     3361 catttcatcg atccgctcga tatagttttt aatatccttt acattttccg cacgctcaat
     3421 gcgatcattc atcacaaact tggtcgaagc accggctcca agtgccagga tcgactgctt
     3481 ctcttccatg atcaggatat tgtaaatgcc ttctttttcc ggttttgcat atccgacatt
     3541 ctcaaaattg cccttcatat tcttctggcg gtagagataa tatgggaaca taccggcttt
     3601 tgccgcatac tgctccgtca tctccatgat cctctgattg tttgtgaaag tcatctcttt
     3661 atattcatct ttaaacaggt tgagacgtgc cgcacgtttg atggccagag aatgtacggt
     3721 gatgctgtcc ggatcaagag ataacacctc ctgcatggtg tgctccacct cttcgatgcc
     3781 ttctcccgga agtcctacga tcnnnnnnnn nnggcaaatg cctttcggat gtcttccacc
     3841 gtatgccgtc ttccaatgat atccagcgtc ttctggttca tcgtctgcgg attgatggaa
     3901 atcctggtca ccggatgctt tcggatcaca gccagcttct cccatgtgat actgtccggt
     3961 ctgcccgctt ccacagtata ctcttttatg tggctgatat caaaacattt ttccacttca
     4021 gacaataaac gctccagctg atgcggctcc agcgtggtcg gcgtgcctcc tccgatatag
     4081 atcgtatcca ggacctgacc ggcccacata ctcgccgttt cccggatttc atggcaaagt
     4141 gcatccagat aatcatcgac cttatttttc cacactttta acggactgga actgaacgaa
     4201 cagtatgcac agatcgttgg gcagaatgga atcccgatgt aaaggctgta tccgttttta
     4261 tagtcaatat cctccaggat atgacgttct ctgttggcaa ttgaaacagc cagtgccgtt
     4321 ttttccctgc tgaccagata ctgctccctc atgatctttg ccgtctccac gttagaatgt
     4381 ccggcatcga tcagtgccat cggtattttc gtcggccgca caccggtcag tgttccccac
     4441 ggaagttcct gtccggttct tttctgcagc atatggtaca gcaaaatttt tgtctgattt
     4501 tttctttctt tccgcccgtg catctcttct gtgagccaca cgccttcttc ctgacgtttc
     4561 cggtcaccgc ttacacgctc gatgcggatg cttatcttat ttttcccgta aaaaagagag
     4621 attttttcat cgtattccgc tgccggactg tcatcgtcag acaaaacgat ctcttcttcc
     4681 ggataaaacg atttcaccag attgtannnn nnnnnnatca tactcaaaat ctctcttttg
     4741 gaactgtatt gctatcatct gttctactca tttcattttt atttgcaaac gttccggtcg
     4801 gcacaaagcc atgatccacc ggacaatgtg tgcctcgcgg agcgtttatc ttagtcggag
     4861 tcaaaactcc cgctaagata aatacgggtt aaatctttgt tcataggcaa tcgtcgttat
     4921 ttcctggtgt cccggataaa cgactgtatc acccggcaga ttctccgtca gatactgcac
     4981 ggaacgcacg atctgtgaca tgctgccggt cggaaaatcc gttctcccga cgctggtgcg
     5041 aaacagtgta tccccggaaa acagtactcc ttcttccgac agataataac agacaccgcc
     5101 ctttgtatgc cccggtgtct cgatcacctt aatcttaaac cctgcaagag tcaaagtttc
     5161 cttatctctc agcagacggt ctgcacggat cgacagagca gcagcccagg aagcagaaag
     5221 attgtaactg ccattttcca gtacttcctc ttccttctcg ccggcataac actgtacacc
     5281 tttaaaatat gcttttaaat catccaccgc accgatatga tcaaaatgac catgcgtaag
     5341 cagaatcccc tgcaatttca ggccatccgc ttcaatcttc cggatgatcc gcccggcatc
     5401 atccgccgga tcgatcagga tcagttcctt tgttttctca tttatcacaa accaggtgtt
     5461 ggtctgcacc atgccaagca ccatcttttc tatttttata tttcccataa acgcctcctc
     5521 tgatcagcct gaagtacgct ggatatcgat cacactctcg atctgacgta tcttggcagt
     5581 caggcttgca agctcttctt tgccc
//
LOCUS       DAWDWH010000135         5527 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6219_length_5527_cov_7.694810, whole genome shotgun sequence.
ACCESSION   DAWDWH010000135 DAWDWH010000000
VERSION     DAWDWH010000135.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6219_length_5527_cov_7.694810"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1058..1067
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1632 a         1077 c         1487 g         1321 t
ORIGIN      
        1 atcggatcga ggcagaagca aacagggagc gtatcctcag gtggggaccg cgtactttgg
       61 atctggatat tctgctgtat gatcaggaag tcatcgacac agaagagctg catattccgc
      121 atattgatct gcagaaccgg gattttgtcc tggtgccgct ctctcagatc gcaccgtggg
      181 tacgtcatcc ggttttgaac aggacaatag gacagttaag gagagaactt gatggttgat
      241 ttacaggact taagaaatga gatagactcc attgaccgtc agatgacgga gctttttgag
      301 aagcgtatgg agatcagccg aaaagtggca gagtacaaga tcaacaccgg caaaaaggtg
      361 tttgataagg aacgtgaaaa aaaagtcatt gaaaaggcaa aagatatgac acacaacgac
      421 ttcaaccgtc acgggattga agaactgttt cagcagatca tggcgatgag ccgtaaactg
      481 cagtatcagc tgttggagga aaacggtgtg gcaggccgcc tgccgtttat cggcgtggat
      541 gagctggaga aagagaatgt ccgtgtagtg ttccagggcg tggaaggagc atacagtcag
      601 gctgcaatgc agcagtattt tggcgataag atcacctcgt tccatgtgga gcactggaga
      661 gatgcgatgg aggcgatcgc agaaggtgcg gcagactatg cagtgctgcc gattgagaac
      721 tcaacggcag ggagcgtcaa tgatatgtat gacctgctgg tagagtttga aaactatatc
      781 gtcggtgagc agatcattcg ctgtgagcat tgtctgcttg ggcttgaaaa tgcagaaatc
      841 tccgatatcc gtactgttta ttcccatcag cagggacttt cccagtgcaa gagctttctt
      901 caggaacacc gggactggaa acagatcccg cttgagaata ccgcaatggc agcgaaaaaa
      961 gtggcagagg acggcgatgt gacgcaggca tctgtttcca gtgcctttgc ggcaaaatgt
     1021 ttcgggctga aagtgttaaa agaaaatatt tacagcannn nnnnnnngaa aatgtgttca
     1081 gtcatgcaag cgagatccgc ggcgaggtgg atggcagcat caatgcattg gtggagcatg
     1141 attatgagat ttttcgtgaa ctatatgccc taaactttga ggcactggaa gaaaagctgg
     1201 gtgcacattg ctttttcatg attgcagatt atgttaatgc accgcagagc agcaaagtat
     1261 ttaacgagcg tattcgcgac cggatctgtg aactgagcga aaaacttgca ggagcaaaag
     1321 atgtgcatga atttaaagaa gatatgactt ctttctacaa agatttcggt gtgggaaaac
     1381 tgggactgca taaggcattt cgcgtggcac atgatgaaga tggcgtacag atcgtgccga
     1441 tcaccaagat cgcacacgta cacctggatg acctggtagg ttatgagctg gcaaagaaga
     1501 aactgatcga aaataccgaa gcttttgtgc agggaagaaa agcaaacaac tgtctgcttt
     1561 ttggtgatgc cggcaccgga aaatcctcca gcatcaaagg cattctcaat gaatattacg
     1621 accagggact gcgtatcatt gaagtttaca aacatcagtt ccaggatctg aatgatgtga
     1681 tcgctcaggt caaaaacaga aattatcgtt ttattatcta tatggatgat ctttcctttg
     1741 aggaatttga aatcgaatat aaatacctga aagccgtgat cgaaggcgga ctggaacgca
     1801 agccggaaaa tgtactgatc tatgcgactt ccaaccgcag acatctgatc cgtgaaaaat
     1861 tcagtgacaa ggaagagcga agagacgatc tgcatacttc tgatacggtg caggagaaac
     1921 tgtcactggc atatcgtttc ggactgaaga tttattttgg ttcaccgagc aaaaaagaat
     1981 ttcagaatat cgtccgcgtc ctggcagaca aacacggaat tacgatgtcg gaagaagagc
     2041 tgtttttaaa ggcaaatcag tgggaactgc agtatggcgg actttccgga cgtactgcac
     2101 agcagtttat tgatcatctg cttggacagg agagaaagga cgtgtgagta tgaaactttt
     2161 tgcagcaatc gatataggtt cctatgaagt ggaaatgaag atttttcaga tcacttccgg
     2221 aaaaggaatc aaagagatcg actgcgtgtg ccatcgcatg gaacttggca aagatgtata
     2281 ccgtgatggc aggatcggtg tggagatgac cgatgagctc tgccgggttc tgaatgactt
     2341 tgtaaggatt atgaaaggat atcgcgtgga tgactggcgt gtgtgtgcca cgagtgcagt
     2401 aagagaagcg ggtaaccagc ttgtttttct ggaccgtgtg aaacgccata ccggtctgga
     2461 agtggaagtt ctctcaaatg catcacagcg ttttatggat tataaatcga tcgcatccag
     2521 ggaaaatgaa ttcaaccgta tcatccagaa aggaacggcg atcgtagatg tcggcggcgg
     2581 cagcattcag atttctctgt ttgacaagga tcgtcttgtt gcaagccaga atatgaaaat
     2641 cggaaatctg cggctgcgtg aacggatgat gaatgagggt gtgagcctga agcattatga
     2701 gacacttctg acagaagttg tgaaaaatga atttctgtca tacaaaaagc tgtatctgaa
     2761 ggatcgtgtg atctccaatt tgatcgtggt cggggaccat atcggcgatg ccgttcataa
     2821 aaatgtgatg acaagagcgg atttcctgaa tttttacgat gacatgatct accgttctga
     2881 cgaagatgcg gcaggacgct gcgaggttcc ggaagatgtg atctcggtat tgcgcccgtc
     2941 tcttgtcgtt taccgttgtt tcctggaggc agccgatgtg gagaatatct ggatgccggg
     3001 tctgcacctg actgatggca tggcgtatga atatgcacag cagaaccggc ttttgaaact
     3061 cggccatgac tttgatgagg atatcctggc ggctgcaaga aatgctgcca aacgttatca
     3121 gtgcagcaag tcgcatatca aagtgtgcga agatctggca gttggtattt ttgacaagat
     3181 caaaaagagc cagggattcg atgaccggga tcgcctgctg ctccgcattg cggtgatcct
     3241 gcatggatgc ggtaaattta tcagcctgtc aaatgccggg gagtccgcgt accatattat
     3301 catggcgatg gatatcatcg gattatcgga agaagagaaa gaaattgtgg caaatgtggt
     3361 aaaatataat acggtgccgc tggaaggata cgacaagatc ggaagcagcc ggatgacaag
     3421 ggaaaattat ctgaccattg ccaaattgac ggcaatcctc ggagtggtaa attcgctgga
     3481 ccgcagccac aagcagaaga ccaaagaggc aaaacagata ttgcacgaaa acgaactggt
     3541 cattcagctt tattcggacg aagatctgtc actggaaaag aaagagtttg caaaaaaagc
     3601 agaatttttc gaggaagtgt tcagcatccg tccggtgctg cgtcagaaaa aaatgatgta
     3661 acgaaggagg tatgtccgta tgggtgaaat ggatttaaaa aaagcagaat actatacaaa
     3721 ccgagaatta agctggctgg gttttaacaa ccgcattttg ggagaggcaa gagatgcctc
     3781 acttccgctg tttgaacggc tgaaattttt aagtatcaca gcatccaatc tggatgaatt
     3841 ctttatgatc cgtgtggcat ctttgaaaga tatggtgcac gcaggttaca caaaaaagga
     3901 tatttccggg atgacggctt cggagcagct gaaaaaaatc ttaaaagaga cacacgaact
     3961 ggtagatctg cagtattcta cttacaatcg ctctttgatc ccggctctga aaaagcgggg
     4021 gctgaaaatt atttccgagc acgagagtct gagcgaagag cagaaaaaat ttgtggatgc
     4081 ttattttgag gaaaatgtct atccggttct gacaccgatg gcgatggatt cttccagacc
     4141 atttccgctg atccgcaaca agaccctgaa tatcggtgcc ctgatctgca gaaaagacgg
     4201 caaggaaaag cataagacac tggattttgc aacggtgcag gtgccatctg tcctgccaag
     4261 aatcgtggaa cttccggaag gaaaagacgg agaaaaacgt gtgattcttc tggaagagat
     4321 catagagcgg aacatcggac agctgttttt aagttacgat gtcgtatgtg cacatccata
     4381 ccgtgttatg cgaaatgcag atttatccat tgatgaggat gaagcagagg atctgctcaa
     4441 agagatccag aaacagttaa agaaacgtca gtggggcgaa gtgatccgtc tggagatcga
     4501 aggaaaaatc gacaaacggc tgttagatat cttaaaagaa gagtttgccg tgaaagacag
     4561 cgatgtattt gagataggcg gaccgctgga tctgactttt ctgatgaagc tgtacggcat
     4621 ggaagggttt gaagactgca aagtgaaaaa atatattccg cagccggtac cggcactgat
     4681 gaacgatgac agcatttttg ccaatatccg caaaggtgac attttactgc atcatccgta
     4741 catgacgttt gatccggtcg tagattttgt aaaacaggca gcgaaggatc cgaatgttct
     4801 ggcgattaag cagacacttt atcgtgtcag cggtaattca ccgatcattg cagcactggc
     4861 acaggcggca gagaacggca aacaggtttc tgttctggta gaattaaaag cccgttttga
     4921 tgaagaaaac aatatcatct gggcgaaaaa gctggaaaaa gcaggctgtc atgtaatcta
     4981 tggactggtg ggtctgaaaa cacacagcaa gatcaccctg gttgtccgga gagaagaaga
     5041 cggtatccgc cgttatgtgc atctcggaac tggaaattat aacgattcta cggcaaaatt
     5101 gtatacggac tgcggtcttc tgacctgtgc agaaccgata ggggaagatg cgactgcggt
     5161 atttaacatg ctctccggct attccgaacc aaaacactgg aacgacctta tcgttgcacc
     5221 gatctggctc cgcaaacgtt tcttaagact gatcgaacga gagaagaact gggcactggc
     5281 gggaaaagaa gcccatatca ttgcaaaaat gaattccctg tgtgaccggg agatcatcga
     5341 ggcactctat gcggcatcgg cggcaggggt gaagatcgaa ctgatcatcc gcggcatctg
     5401 ctgtctgaag gtcggcatcc cgcatatcag cgaaaacata tcggtacgtt cgatcgtcgg
     5461 caatttcctg gaacatgccc gtatcttcta tttctataat gacggggcag aagagttgta
     5521 tatgggc
//
LOCUS       DAWDWH010000136         5471 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6283_length_5471_cov_15.877031, whole genome shotgun sequence.
ACCESSION   DAWDWH010000136 DAWDWH010000000
VERSION     DAWDWH010000136.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5471)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6283_length_5471_cov_15.877031"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3598..3607
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1833 a         1078 c         1441 g         1109 t
ORIGIN      
        1 atttcggagg aatcgcaaag ggctatactt ccggaaggat catggatatt ttccgtgaac
       61 atggcatcac aagcggaatg gtaagccttg gcggaaacgt acaggtactt ggaatcaaaa
      121 cagatggaag caaatggaga gttgccgttc agaatccaga agatacggaa aattatcttg
      181 gtgtccttga gacacaggat cgtgcggtga tcacgtccgg cggttatgaa cgctattttg
      241 aacaagacgg caagacctat cagcatatac tggatccgaa aacaggatat ccggcagaca
      301 gtggactgac atctgtgacg atcgtcagtg cggacggaac gctggcagat ggtctgtcta
      361 cgtcactttt tgtgatggga aaagaaaaag cagtggcata ctggcgggca cacagcgaag
      421 aatttgacgc gattctggta gaaaaagacg gaaccgtatc ggtgacagag aacattgcgg
      481 atcagtttac accggatgat gcggtaaaaa agacagaaat cataacaaag cattcaagtc
      541 gaacgcacgt gagacttggt gcgtgattct ggtcagcgaa agctgacata ttcttctcag
      601 aatcactgca catcctcgcg gagcgtttat ctcagtcgga gattatactc ccactgagaa
      661 agacttggag tgttattact cacttgcaga agtgggagtc ttctcgactg agataaaaga
      721 caagaatcat aagactggag gagcggtaag atgaactgga aaaaattatc agcgtttgca
      781 ccgatgctgg cggttattgt actggccgga tgcatgggaa aaacattagc gggatatacg
      841 ccggccactt acgaagccaa agcagaagaa atcacactcc aggaagaaac aacacaaagc
      901 acagaagaaa aaggacagca aacgtccaaa ataaagaaaa aaacaaagaa agccgcaaaa
      961 acagaaagca aaaagaaagc ggcaaaagga aactttgatc tggaagatgg aacttatatc
     1021 ggggaaggtc agggatttgg cggaacgatt aaagtttcgg tcgtaataaa agacaagacg
     1081 atcacagaaa tcaatgtcgt gagtgccgaa ggagaagatg cggctttctt tggaagagcg
     1141 aagggcgtga tcgataagat cctttccggg cagaagacgg atgtggatgt ggtatccggt
     1201 gcgacctata gttccagagg cattataagt gccgtgaaaa atgcattgac cggagagaag
     1261 gataccagta cttcaccggc agcgggcggc acaggcgggg catcggcagc agccggaagt
     1321 ggaaagatca cgcaggtggc ggaagacaaa gatcagacct gcaaagacgg cacttactat
     1381 ggcaccggaa ccggatttgg cggaacggtg aaagtaaaag ttgtgatcaa agataacaag
     1441 atcaaaagta ttgagatcgt ggaacatcag gacggaagca gctatatgca gaaggcatcc
     1501 gcactgatca gcacggtgat cgaaaaacag agcaccaacg tagacggcgt gtccggggca
     1561 acttacagtt cggccggtct gatcgaggcg atccgaaatg ctgtagatca ggcaaaaacg
     1621 acaaagacaa ccggggacaa taagaaagaa accgagaaaa agaataacga tacatctgga
     1681 actgtcactc cacaggtgac tggaaagttt ccatataaag atggtatcta ctatggaacc
     1741 ggggaaggat atatgggaga tatcaccgtt gcagtggtca tccagaataa gacagtcacc
     1801 tcaatcctgg tgacagagac agacggagac ggggaagaat ttgtcaaaaa ggcaaaagca
     1861 gtggcagacc agattgtaaa aaaacagagt accaggaaaa tagataccgt gtcgggagca
     1921 acttacagct cgaagggaat cctggaagca atcaaagatg ccctgaatca tgcaaaagag
     1981 gcaactaacg ggaaagaaac tgaaaagccg gcagaaaccg agacgcagaa gccagcagaa
     2041 accgagaaac cctcagaaag tgaatcttca tcggagagtg aaaaagaaag agtgtatgcc
     2101 gacggagatt ataccgcttc ggcaacctgc acttcctctg gcggaggagc gtttgacgat
     2161 tatacactga ctgcgaccgt cacgatcaaa gatgacaaga tcacagcgat cacaaacgtt
     2221 gccggaagcg gagcaggtta tgtggctgca gatgctcttt atataaagag ggcggcagaa
     2281 ggcacaagaa aagcacaggg agtggtagca caggttctgg caaagaacag cttggacgag
     2341 atcgatgtgg tatccggtgc gacctgttca tccaatgccc ttttagaagc ctgcagacag
     2401 gcacttgaga gtgcaaagat cgtgcaacaa agtgagtaaa aagtaaggaa gattctgatg
     2461 aaaaaattct ggataaaaat gtgcagcctt gtgctgctac tgtttctaat cggcggttac
     2521 aataccgtgc ttgcaatgcg ggagcagcga gatgagatcg ccaggctgac cgcagagctg
     2581 gagggcagca agatgaccgt ttcagcttta aaggaacagc aggcaaagaa aacagaaaat
     2641 acagcggcag aggctttaaa aggtgaagat gccaagaata cagacggagg ctggaaggac
     2701 ggcacttacg aaggggaagg tcagggattt ggcggcaagg tcgttgtgga agtgacgatc
     2761 gagaacggag aaatcacgtg cattgaagtc aaagaagcac aaaaagagga cagtgcatac
     2821 cttgaaatgg caaaggatat catcgaggat atcgtagatg cacagtcggc ggacgtggat
     2881 actatcagcg gagcgacctt cagttcaacc ggaatccggg aagcagttat acaggcactt
     2941 gaaaaggcgg aataagaaat gaacaagaaa cttgacagaa aaaagcaaaa acaagtacat
     3001 cgctatgtaa gagctaccat acagctgatc ttctttctgc tggtaccatc cgcttttaca
     3061 gctgcatttt ccggtgtaaa atatctgttt acgcagattg gcaaaggaga tgcgatcggc
     3121 atgacaagtt ttgtcacagc tctgtgcgtc ctctgccttt ataccattct gttcggtcgg
     3181 tttttctgcg gatttgcctg tgcattcgga agtttcggag atgcactgca tggtatttat
     3241 ctgtggatct gtaaaaaaat caggaaaaaa ccgtaccgta tttcagagaa gatcacaaag
     3301 tatttatatt tgctgaaata catcgtgctg gctatgatca gcctgttatg ttatgcaggc
     3361 gtttatgcaa aaacaaaggg caccagtccg tgggaagttt tttccatgat ccgtgccgga
     3421 aacttcaaac ttggcggata tgccatcggg attgtactgc tgaccattct gatgatcggc
     3481 atgtgtgtgc aggaacgctt tttctgccgt tttttctgtc cgatgggtgc agtcttttcc
     3541 ctgcttccgg cactgccgtt cctgacactg gcaagagacc gtgaaaactg catcaaannn
     3601 nnnnnnnatg cacgaaaaac tgtccggcag aactgggact tccaaagagc ggtttgatcg
     3661 agacgccggg agaatgtttc cagtgtcaga agtgcgtgga catctgcccg aaaggaaata
     3721 tccatacgag agtgccgaaa cttcgcggca atgaagtgtg gtttacggtt ttcagagcat
     3781 tgctgctgct tggcgtgctt tacctgttcc atctggcata ggcaaaaggg agtagccttc
     3841 ttttatgccg ggcggaacta tttttaaggt tattggttag cgaatgctaa tttttaataa
     3901 aacaaactaa ttattattcg aggaggaata ctcatgaaaa aaagaaccat caacatgaaa
     3961 aagtcactgg caatcgcagt cgcagcagca atgatgctcg gtatctgccc gacagcagca
     4021 ccggtcaatg cagcagattc agattacaaa tacgtttatg caagtctgga ctgggcagag
     4081 tactggcaga atgaaaatgt ttatctggca agcggaagta cactgaaatc ttcctccgaa
     4141 gaagcggatg ccagaggaga atatgataaa ggtgcatttg acgttgtaac gcgtgcaacg
     4201 aaaaaccacg gactgcaccg cggaagtttc cagtgtgatg ctgtgatccg tgataccgat
     4261 ggaaaagcat atgaaatctc ttactggaca tccgaagaag tgtcggcaaa ggatgaaaat
     4321 ggacaggata ctacgaaaag agttgtctat gcagtcctga cagacggaaa caagatccag
     4381 tttgataaag gtacgatcac ctacaccgaa aacggtgaga caaaaacggc aaccatggag
     4441 gattatgaag taaaaggaat caaatatgta ccggttgcag tcaaaacaga agattatgca
     4501 gatttctgca agaaatacag cgtaacagaa gatgcatctg caatcgaagg cggatatacg
     4561 gagaaagcac tgaaagcata tacaaagaca gcagaagtaa ctgcagatac caatggtctg
     4621 aaagttgcaa caaaaaatgc agacggaacc tattcttttg cggcaagaaa gacaggaagc
     4681 aattccggta tcaaaaatga agcacagaaa acagtcgatg agacaaagat tgtaaagacg
     4741 gtacgttccg cagatgatgc tgagacttac ggtgcattta tccgtgttga cctgacagga
     4801 gatgcttatg gtgatctggg tgcaaatatg cagacagttc gctgggacta ctatggaaat
     4861 gacagcactt acacaaacaa actggcaacc tacggaacaa aatttgcagc agacaactgg
     4921 atgcacaaat ccatgggtgt tcagcttgga ctgacgaaat cccttcgctg ccagctgccg
     4981 gaaggaaccg acggaaccgg atactggaaa gtaactgtat atgcactggg atatcaggat
     5041 tatacctttg aagtacaggc aacatctgag aacatcgtat ctgacaataa aggtgatgag
     5101 aaagtagata ccacagaact gcagaaagtg gtagacgaag caaagaaact ggtaaaagca
     5161 gactacaccg cttctagctg ggcatctttt gagacagagc tggcagaagc agaagatgag
     5221 ctgaaaacac cgcatacaca ggcaacagta aacgaagcga tcgctcatct gcagaatgcg
     5281 atcaaagatc tggtaaaagt acagaaagaa accgagacaa agacaccgga aacaaaaaca
     5341 gatatcaaca atgataagaa caaccagaca cagactgctt acaaagcaaa agtaaaactg
     5401 aactctgtca agaacacaaa aggcagaaaa gcagtcctga aatggaaaaa ggttaaaaat
     5461 gcagatggtt a
//
LOCUS       DAWDWH010000137         5407 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6350_length_5407_cov_13.519619, whole genome shotgun sequence.
ACCESSION   DAWDWH010000137 DAWDWH010000000
VERSION     DAWDWH010000137.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6350_length_5407_cov_13.519619"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1773..1782
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1857 a          807 c         1286 g         1447 t
ORIGIN      
        1 tacaatgcgg ttcgtctgca cattgccaaa agctcctggc ctgatggaga ctctgaggcg
       61 gaagattatt aaaaaaatat ctgtctgatt tggattgaca aaaatcgctt attcaagtat
      121 aataatcaca aaatagtttt aaatctaatt ctatctgact ttcattgccg ttcggcagga
      181 aaatccaaaa aaactttata cctgcacaga gcggctgaaa atacaaaagt gatatcttaa
      241 gaaaactttt tgacaaccga tatttgcaaa ttgcttctta gccaaagagt ttcacaatcc
      301 cggtatccaa ggctgttttg tagcagggaa atgtcagcaa agctgaccag aagcgtgcac
      361 cgggtctcac gtatatttag cctggattca tacaaaaagc aaaggagcag cctatgggac
      421 aaaaagatat tacagagaaa ctactggaag attataatga tgtatttgca gatatcataa
      481 acggactgat ttttaaagga gaacagcgga ttctgccgga atctctggaa aatgcaaaag
      541 tccactctca atataaagca gaagatggaa aagtccatga actggagagg gatgtcataa
      601 aatactggaa agagaaaaaa gtcgaactgg cgatctgcgg gatcgaaaac cagagcaatg
      661 tgaaaaaata tatgccgttc cgtattatcg gatatgatgg tgctgcatat cgcagccagc
      721 tattggagaa cagaaaagag atcctgccgg tcatgacaat tgtattgtac ttcggtacgg
      781 atcatcactg gtatggcaaa aagaatatca aagggttgat gaaaatcccg gaaggactgg
      841 aagagtacat taatgattat gaaatgaaag tatttgagat cgcatggctt acagaagagg
      901 agatcagccg tttccacagt gattttaaag tcgtggcaaa tttctttgtt caaaaaagaa
      961 agcataagga ttatatcccg gatgatccaa cggaaatcag gcatatagat gaagtactaa
     1021 aattgctccg ggtaatgacc ggggatgaac gctaccaggc aatatttcag aaagagagag
     1081 gagtgcatag tatgtgtgat gtggcggaaa gactcgaaaa aatgggaatg gaaaagggaa
     1141 tagaaaaggg aatggaacag ggagtgaaac aaggaagaaa gttaggcaga aatgagggaa
     1201 tccacgaaga aaaagtccgt atatacaaaa aacttcttga aaaaggattt tcaaaacaag
     1261 aagcacagga gataaccgaa ttgccgcaat ttctgaaaat gtaaatttgc acatgcaata
     1321 ctattctttt gcaaacacta ttttaaaatg tgcggcattt cagttcttta cacctgccag
     1381 gaaaccaacg gtaaattttt atttcttgcg aaaaatcgtt ctgaagatca atctcaccag
     1441 cggtccgcag tagaacatct gccagcagat tgccatcggg aagttcatgg cccaggtctg
     1501 gatccaggtg ccgaatgtgt gctggtcttt aaagagaata gtggcaacca ggctcatgat
     1561 cggacacata atgcagcaga tgcagataga aatggcatag gtgatgaact gtggacgatc
     1621 ggtaggtttc attacggtga atgcgagttt ctgagcaatt tttccgacaa taaaaaattc
     1681 cagaacgaag gcaaccggaa ccataatgat catttcatgg gtagccagca gaaaggtctg
     1741 accggataca ccaccggtat tcagggctac atnnnnnnnn nngaagtaga gaaggtatta
     1801 aaggatatcg aagcaattcc gagtttagaa aaatttatag aggatcattc ggaataaaaa
     1861 gcagaatgta aatttgataa aatatttttc acaataaaac aaaggcgatc aactgttaca
     1921 tcagatggtc gcttttgttt ttggcaaaat gtgcgaaaaa gaaagcttgt gcagtctgta
     1981 aattctcgtt atactgagtg tgatgataga aaaaggaaac ttgtgaaagg attttagctt
     2041 taaagggaaa tgtaaggtaa aaggaggggt acgatgggta ttttttttaa tcccggaaat
     2101 gggagtttta ccaaagatag aaattatgaa atatatgtgg ataaaacaga attactaaaa
     2161 tacttgaaca gagccattgg aacacctgga aattgtattg ctgtcagcca tgccagacgc
     2221 tttggaaagt cacatgctgc ggggatgatt gacgcttatt atagtcttgg tagtgattct
     2281 gcaaaattgt ttaaaggtac aaagattgca tcagatccag attatgaaaa atacagaaat
     2341 aagttcaatg taatccattt ggatatttcg tccttttggg attttcataa aaaggatatt
     2401 gtagaaaaga taatagaaga tatttttaaa gaattgaaaa tggattaccc ggaggaattt
     2461 aatgaacggg atcgtatcag ttatgtactc ataaaaatat acgaaaaaac agggattcct
     2521 tttgtgatta tcattgatga atgggattgt gtggtacgaa acagtacgga tcaggatttg
     2581 atccatcaat atttacagtt tctccattct ttgtttaagt cagaagaatc aaaatctttt
     2641 ttggcacttg gatacatcac aggtatcatg ccaattaaga aaataaagga tgaatcggct
     2701 ttaaacaact ttgaagaata tactatgcta aaatccagac cgattacgaa atactatggc
     2761 tttaccgaag aagaagttaa ggatttatgt aaaagatatg atatggattt tgaaactaca
     2821 aaagaatggt acaatggata tctgatcgat ggaatgcaca tgtataatcc aaattccgta
     2881 tcacaggcga tgaaatatca tgattttgat tcctattggc gaaatacctc tgcttttggc
     2941 acaattaata actttattat gatgaattat tcaggattga aagaggatgt ccttacaatg
     3001 ctatccggag gaaaagtcat ggtggatacg gaatgtttcc aaaatgatct ggcagaaatt
     3061 cattcaaaag atgatgcgtt aactgctctc attcatcttg gatatcttgg atatgatgca
     3121 gatattttaa gtgcctatat tccaaattat gaagtggcta aggcgtttca atccgcgtta
     3181 aaaacaggag aatggaaaga tgttgctgcg tcgatttcca aatgtgatac attacttatg
     3241 gcaacgatta gtggtaacac tgagaaagta gctgaactta tcgagcttgc acatgatacg
     3301 tatacttcta tattaaagta caacgatgag aactctttaa gctgtgtgct tacaatggcg
     3361 tattttactg caccggcata ttatactatt attcgggaaa tgccggccgg aaaaggattt
     3421 gctgattttg tttttttgcc aagggcaaat gctgggaatc gcccggcaat gattgtggaa
     3481 ttaaaatata accaatcagc agatactgca atcaggcaga taaaagagaa acgataccaa
     3541 ggtgcgttag tgggatatga aggcaagatt ctcttagttg ggattagcta tgatagtgaa
     3601 aagcatcata cttgcagcat cgaagaatgg taattttgtt atactgagtg tgatgatagg
     3661 aaaaagggaa acttgtgaaa ttgttttgat ttcacaggaa atgaaaagta aaaaaggagg
     3721 ttcacatgaa aaaaagaatt ttatgtacgc tgctgagtgc agcaatgctg cttacgagtt
     3781 cgccggttgt ttttgcggcg gagacgcagc ctgtgaagga gcagcagacg aaggagacgc
     3841 ttccgtttac gattaaggag gcaaacaatg atattaaaga tgaaagtgta aaaggctggc
     3901 atgttcagat cacggatacg gaaaatgcaa aggttgttgc agattatccg ggactggttg
     3961 ttgtgcgtgc aaagaagaca cagtacgatg acaatgttta tctgatcgga aagagtggag
     4021 ttgttcagac ttttgtggtt tcgggcacgg atgcagactg ggatgaaaag gaacatgttt
     4081 atgacgattg ggagaaaacg acgtcgcata gttctgtgga tgcgatatgg agcttttcgg
     4141 atgagggaga tcttttatat tatattaaga agccacagac gcagactccg aagtatgatg
     4201 tttacaatgc gacaacgggg gaatattttg agtatgatct ggatgagaaa ccaacgtatt
     4261 atggtgatgg aaaaggatat tcggcaggtg tttatcgaat tgtaaaagac ggaaaaacag
     4321 gaatgctgaa gaatgatgga agcattctgt atgaggcagt gtacgatgaa gttgacaggg
     4381 ctgcatataa tcaacatttt tctgatggaa tggaagaatt agatgatctg ctttatatgg
     4441 ttacgcagga aacttcggat gaggaattat atggttttat agattccaaa ggactttggg
     4501 atggtgtgtg cagatatgaa tcaacatggg atctgagtgg ctacagaaaa aaggtaaaag
     4561 ataaagacgg tggctatatg atactggatc tgaaaacagg cgagaaattt gcagaccaga
     4621 tcagttccgg cggtgatatg aaagctctgg gtgatggatg ttttcgtatc gtaccggata
     4681 atctggaaac gaacatggat atcctgtatc tgacaaaaag tggaaatctg atcacaagag
     4741 cacagcttta caacagcgat ttctgtgatg atcaggattg tgagattttt atggaagaat
     4801 tttacggttc tgctattgcg gaacatggaa gctatctggt agcctatgat gcagatggga
     4861 agaagcactt taaagactac atagatcaat ccggaaaaac agacaatatg tatgtttcat
     4921 acagcagata tgaagatgga tatgcgatta agcttgagtg tgcggacggt aacccggact
     4981 acagttcaac gatgagggag tcaacggtat tatgcagtct gattgatgca aaagggaaaa
     5041 agatttattc atttgacaac aataccgagg ttgagataaa agaagtgcgg gatggtttga
     5101 tttatctcca atataatcca tatggaacaa atgagatcaa tgtgcttatt tatgatacag
     5161 cggagaacaa agtagttttg aatgaaaaga atctggactg ttctgcaagt attccgaggg
     5221 atgacagcag ccatgtggtt cttctggaaa aggatggtaa gacggcactg tttaatatgc
     5281 tggacaaaac attttccgga tgggataaac cggaaatgga tccggatgaa agcctgggct
     5341 gggatgacat taaagcctgc aaataaaaag tcaaggctaa attgaagaac attgaaaatt
     5401 ttataca
//
LOCUS       DAWDWH010000138         5323 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6451_length_5323_cov_9.004746, whole genome shotgun sequence.
ACCESSION   DAWDWH010000138 DAWDWH010000000
VERSION     DAWDWH010000138.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5323)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6451_length_5323_cov_9.004746"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2972..2981
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4106..4115
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4979..4988
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1614 a         1106 c         1345 g         1228 t
ORIGIN      
        1 atcatgacct gcattctggt actggaaaat acagatccat ccgatacggc agcagcctcc
       61 gaaaacgcag ccatgcagcc aaaagtacac cttggtgtac aaaagggaga aactttttac
      121 gtaaaggatc tgctttattc tctgatgctg gagagccaca acgatgcggc tgtgatcctg
      181 gcagaaaaga cggcaggcag tgtggaagct tttgcggaaa aaatgaatca gaaagcagaa
      241 gagatcggat gcagcgacac gcattttgtg acaccgaacg gactcgatga cgaagatgcg
      301 ggcggagaac acgcaacgac ggctgtggat cttgcaagaa tcctgcgtta ctgcatcatg
      361 cagtcaccga aaagggatga atttcttgaa atcacccgca caaaaacgta tgaattttca
      421 gatgtagagc agaagaaaca ttacagctgt tacaatcaca atgcattcct cgatatgatg
      481 gaaggggcac tgaccggaaa gactggattt accggaaagg caggttactg ttatgtcggg
      541 gcactggaaa gcgaaggcag aacctttgtg gtggcactgc ttggctgcgg ctggccgaac
      601 aaccggagct ataagtggac agatacgaaa aagctgatgg attatgcaaa agaacatttt
      661 tacatccggg aatttgaaat gaacgcacag acaccgcagg tgctggtgga agacgggatt
      721 ccggcaagtg gaaagatcaa agatccatgt cttgcagaca aaagagcata agatccgcct
      781 tcttgtatcg gatgaagatg aggtgaagct ggtctgccat atgaaaacga aaacagcggc
      841 tccccttgaa gcagatacgg tgatgggaaa cgtaacgctg tttttaaata atgaaaaaat
      901 caaagaaaac gagatcgtga ctgcggatgg ggcagaacgc atcagtctgt cgtggtgtgc
      961 cgggcagatc ataaaaaagt ttttcatatc ctgatgatga cggaaaagaa aaggttagaa
     1021 tatcattcgg tcattcttac atgcaaagta agaggtttga ggctatgttc aaaattcaac
     1081 gaaagagttt aaaattcaac gaaaaatgaa ataaaatgaa tgtgcatgac ggaacatgaa
     1141 tgaaattcgg attatttctg gtgttgtgct acaaaaaaag tgagaaaaac gagaaaatac
     1201 ttgctaaatc ttccaaaaac cattacaatt acaatcggca gaaaattttt tttcttttaa
     1261 taaattatta atggagggct gaacaatgag taatacagca caaaacttgg caagtacaag
     1321 aatcgcttct ttacttgatg agaacagctt tgttgaaatc ggaggttgtg tgactgccag
     1381 aaacacagat ttcaacctga agcagacagc aacaccgtca gacggtgtaa tcacaggtta
     1441 tggtgtgatc gacggcaatc tggtctatgt ttacagccag gatgcatctg tactgggcgg
     1501 caccatcggt gaaatgcatg caaagaaaat cgcacgtctg tacgatatgg cattaaagat
     1561 gggtgcaccg gtgatcggac tgatcgactg tgcaggtatc cgtctgcagg aagcaaccga
     1621 tgcactgaat gcactgggtg agatttatgc aaaacaggta ctggcatccg gtgtgatccc
     1681 acagatcaca gctgtatttg gtacctgcgg cggcggtatg gcacttatgc cggctctgac
     1741 agattttaca ttcatggaga gcaaaaacgc aaaattattt gtaaattctc cgaacgcact
     1801 ggaaggaaac gaagtttcca aatgcaatac cgcatccgca aaattccaga gtgaggacgc
     1861 aggactggtt gactttactg gcagcgaaga cgaagttctc tcaggtatcc gcaaactggt
     1921 aacgctgctt ccggcaaaca acgaagaaga tgcagtggat gactgcagcg atgatctgaa
     1981 ccgtgtatgt gcagatcttg caaatgcagc aggcgatacg gcgatccttc tttccaacat
     2041 cgcagatgac caggattttg ttgagacaaa agcggactat gcaaaagaga tggtaaccgg
     2101 tttcctgaaa ctgaacggaa caaccgtcgg agctgtagca aaccgtacgg aagtttacgg
     2161 tgcagacggt gaaaaagcag aagagtttga agcagtcctg actgcaagag gagcaagaaa
     2221 agcagccaga ttcgtaaaat tctgtgacgc attcaatatc ccggtactga ctctgaccaa
     2281 tgtaaccggt ttcaaggcag acaaacattc tgagagatgc atcgcaaccg cagttgcaga
     2341 gatgacacat gcatttgcag atgctaccgt tccgaaggta aatgtgatca tcggaaaagc
     2401 ttacggaagt gcctatgttg ccatgaacag caaggcagtc ggagcagata tgacttatgc
     2461 atgggaagat gcgagcattg gcatgatgga tgcaaaactg gcagccaaga tcatgtatgc
     2521 ggatgctgac gcagctacga taggcgaaaa ggcagcagaa tatgcaagcc tgcaggagag
     2581 cgtacagtcc gcagcagcaa gaggatatgt tgataccatc atttccgcag aagacaccag
     2641 aaagtatgta atcggtgcat ttgaaatgtt attcacaaag agagaagacc gcccggcgaa
     2701 aaaacatggc acggtataag tgaggtgaat aatatgaatc gaaaattaaa aagtttattg
     2761 gcgatggtct gtgtactggc tctgacactt gccatgtccg tatgtgcatt tgcagcagat
     2821 accgtgacag aggatgaggc tgccaactac aagtccgcag cagagacact gatctcacag
     2881 atcgcaggat tttctgatga agaaatcgaa aactatcttg cacaggatga tgcatttacc
     2941 acggcgacca tggagtcctg gaaaagtgta annnnnnnnn ntacaccagc tcgacctaca
     3001 gcgtaccgga ggctctcgca gcacagctgc agcgtgccgg aatgaacaca ctgatgggaa
     3061 tcggcatcgt atttctgatg ctgctattct taagctttgt gatcggactg ttcaagtaca
     3121 tagagaaatt ccagaatgtc ggcaagaaga aagccgcaga ggaagcaccg aaagcagaag
     3181 aggcacctgc accggcaatc gcccagagcg aagcggcaga tgaagatttt gcagatgacc
     3241 tggaactggt agcggttatt tccgcagcga tcgcagctta cgaaaatact tcaggagaca
     3301 gttttgttgt ccgttcaatc aagaaatcaa ataaatggca cagagcctga caggaggatt
     3361 tgaaatgaaa acatatacaa tcacagtaaa tggtacagct tatgatgtaa cagtagaaga
     3421 aggaacagga agtgcagcac ctacagcacc aaaggcagct ccgaaggcag caccaaaagc
     3481 agctccaaaa gcagctccga aggcagcagc aggtgcaggt tccgttaaag tagaagcttc
     3541 cgtaccgggc aaagtcctga aaatcgaagc ttctgtagga cagagcgtaa aagcaggtga
     3601 caacatcatc attctggaag ctatgaagat ggagatccct gtagtagcac ctcaggacgg
     3661 aaccgttgca agcattgatg ttgcagttgg tgcagcagtt gaaaatggtg atgttctggc
     3721 aaccatgaac taagatattg gaggttgaat tatgtcatac gtaatcgata cgttgtccaa
     3781 cctgctccat caaacggcgt tttttaacct gacctgggga aactacctga tgatcgcagt
     3841 tgcctgtgta ttccttttcc tggcaatcgc aaaaggtttt gaaccgctgc ttctggttcc
     3901 gatcgcattc ggtatgcttc tggttaatat ttacccggat atcatggcat caccggaaga
     3961 gacatcaaac ggtgtaggtg gacttttaca ttatttctat ttactggatg agtggagtat
     4021 tctgccgtcc ctgatcttta tgggtgtcgg tgcgatgacg gactttggtc cgctgattgc
     4081 aaatccgatc agtttcctgc ttggtnnnnn nnnnngcagc atattcttat atgtcactgg
     4141 tgccgatcat tcagccgcct atcatgaaac ttctgaccac agaaaaagaa cgtaagatca
     4201 aaatgcagca gctccgtccg gtttctaaac tggagaagat cctgttcccg atcatcatca
     4261 cgatcgttgt gtgtatgatc ctgccgacaa cagctccgct ggttggtatg ctgatgctcg
     4321 gtaacctgtt ccgcgagagc ggtgttgtaa aacagctgac agaaactgct tcaaacgcaa
     4381 tgatgtacat cgtagttatc ctgcttggta cttctgtagg tgcatctaca agtgcagaag
     4441 cattcctgaa cctggatact ctgaaaatcg tagcacttgg tctgatcgca tttgcattcg
     4501 gtaccgcggg cggtgttctg tttggtaaac tgatgtgcaa gctctcaggc ggcaagatca
     4561 acccgctgat cggttccgca ggtgtgtctg ccgtaccaat ggcagcccgt gtatctcaga
     4621 aagtcggtgc agaggcagat ccgaccaact tcctgctgat gcatgcaatg ggacctaacg
     4681 tagcaggtgt tatcggtaca gccgttgcgg ccggtacatt catggctatt ttcggtgtat
     4741 aaaacgttat taacttagga ggatatagat catggcagat attgcaaaaa agcagaataa
     4801 tgcaaaaaaa ccagttggta ttacagaaac aattctgcgt gacgcacatc agtctctgat
     4861 tgcaacccgt atgacaacag agcagatgct tccgatcgta gaaaaaatgg atcaggttgg
     4921 atatcattcc gtagaatgct ggggaggtgc aacgtttgat gcttccttac gtttcctgnn
     4981 nnnnnnnnat gggaaagact tcgtaaattc cgtgccggat tcaaaaacac aaaactgcag
     5041 atgctgttcc gtggacagaa catcctggga taccgtccat atgcagatga tgtggtagaa
     5101 tatttcgtac agaaatccat cgcaaacggt atcgatatca tccgtatctt tgactgtatg
     5161 aatgacctgc gtaacctgca gaccgcagtc aaagcagcaa acaaagaaaa aggacatgca
     5221 caggtagcac tgtcttacac cctgggtgat gcttacaccc tggattactg gacaaagatg
     5281 gcaaaagacg ttgagaacat gggtgcagac tccatctgta tca
//
LOCUS       DAWDWH010000139         5314 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6458_length_5314_cov_15.685682, whole genome shotgun sequence.
ACCESSION   DAWDWH010000139 DAWDWH010000000
VERSION     DAWDWH010000139.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6458_length_5314_cov_15.685682"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4522..4531
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1214 a         1376 c         1094 g         1620 t
ORIGIN      
        1 tcttcctctt cgaccgttat cgatcagggc atctacagct tcctgaagca tacgtttttc
       61 gtttctgacg ataatatccg gtgctccaag ctccagcagt ctcttcagac ggttgttacg
      121 gttgatgatt cttctgtaca ggtcgttcag gtcagaagtt gcaaatcttc ctccgtccag
      181 ctgtaccata ggacgcagat ccggcggaat aaccgggata acagtcatga tcatccactc
      241 cggacggttg ccggactcac ggaatgcttc tacaacttcc agtctcttga tgattcttgc
      301 acgtttctgt ccggtagctt cttttaagcc tcttttcagc tcttcggatt ctttttccat
      361 gtcgatggct tccagaagct ctttgacagc ttctgcaccc atgccgacac ggaatgtcat
      421 accgtatttt tctctggctt cctggaactc tctctcagtc agtacctgtt tgtactgcag
      481 ttcgctggtt cctttatcca gaacaatgta gttggcaaaa tacagtactt tttccagtac
      541 tctcggtgac agatccagca tcagtcccat tctggaaggg atacccttaa agtaccagat
      601 atgagatacc ggagctgcca gctcgatatg tcccatacgc tcacggcgta cacttgattt
      661 tgttacttca actccgcatc ggtcacagat aacacctttg tagcggattt ttttatattt
      721 accacagtga cattcccagt ctttgcttgg tccgaagatt ctttcgcaga acagaccatc
      781 tttttccggt ttcagcgtac ggtagttgat ggtttccggt ttcaggactt caccatgtga
      841 ccattctctg atcttatcag gagatgctaa accgatttta atggcatcaa acgtaattgg
      901 ctgatacgtt tcattatttg ttgtttctgg catgtatggt ttctcctttc acttccggct
      961 tactcttcct cgaaaacttc atccgggctg aagattgctt catccggtgc ggactcttcc
     1021 tgtacatcaa ccagctcgtc gccttcaaac tcctgtttgg tatagccgta ctcgccgaaa
     1081 gaggactcct cttttctgtt gtaatctttg ttgtcgccct cgatgacaga acgtaagtct
     1141 gtgtctccat agtcaacagt ttccataagt tctacttctg tctggtcctc cttgagcact
     1201 ttgacatcca gaccaagaga ctgaagctct ttgatcagaa ctttgaagga ctccggaata
     1261 ccaggttcag ggatgttttc acccttgatg atcgcttcat aagtcttaac acgtccgaca
     1321 acatcatccg atttaacagt caggatctcc tgcagtgtat aagaagcacc atatgcttcc
     1381 agagcccaaa cctccatctc tccgaaacgc tgtccgccga actgagcttt accacccaga
     1441 ggctgctgtg ttaccaggga gtacgggcct gtggaacgtg catggatctt atcgtctacc
     1501 aggtgatgca gtttcagata atgcatgtgt ccgatggtta ccgggctgtc gaaatattct
     1561 ccggtacgtc cgtcacgcag tcttacttta ccgtctcttg acagcggtac gcctttccag
     1621 agtgctctgt ggtcacggtt gtcatacaga tactgcagaa cttccggcag aagctcttcg
     1681 ccatgtttct tttcgaattc ttcccactcc agattgacat aatcgtttgc cagatccagt
     1741 gtgtccataa tgtctacctc gtttgcaccg tcaaatactg gtgttgagat attgaatcca
     1801 agtgcttttg cagccaggct cagatggatc tccaggacct gaccgatgtt catacgagac
     1861 ggcacaccca gcggattcag tacgatatcc agcggacggc catttggcag gaacggcata
     1921 tcttcaacag gaaggacacg ggaaaccaca cccttgttac catgacgacc ggccatcttg
     1981 tcacctacag agatctttct cttctgtgca atgtaaattc ttacggactg atttacaccc
     2041 ggtgccagct cgtcaccatt ttctcttgta aagacttttg catctacgac aataccatat
     2101 tcaccatgcg gtactttcag ggaagtatca cgtacttcac gtgctttttc tccgaagatt
     2161 gcacgcagca gacgctcttc tgctgtcagc tctgtctcgc ccttcggtgt tacttttccg
     2221 accagaatat caccggcacg cacttctgca ccgacacgga tgataccacg ctcatccaga
     2281 tctttcagtg cttcatcacc gacaccagga acgtcacgtg tgatctcttc cggtcccagt
     2341 ttggtatcac gtgcttctgc ttcgtattcc tcgatatgga cagatgtgta tacatcgttc
     2401 ataaccagtc tctcactcag cagaacggca tcctcgtagt tgtaaccttc ccaggtcatg
     2461 aatccgatca gagggttctt accaagagcg atttcgccgt tggatgtgga aggaccgtct
     2521 gcgataacct gtccggcagc aactctgtcg cccttgaaaa cgatcggacg ctgattgtag
     2581 cagttactct ggttgcttcg tgaaaactta gtcagacgat actcatctct tgtaccatca
     2641 tcgttgcgaa ccacgatacg tttggattca gaagactcga tcgtaccatt tttctttgca
     2701 accacacata caccggagtc aaccgctgtc ttttcttcca taccggttcc tactacagga
     2761 gcttcggttg tcagaagcgg tacagcctga cgctgcatgt tagatcccat cagagctcgg
     2821 tttgcgtcgt cgttctgcag gaacggaatc aatgcagtag caacggaaaa taccatctta
     2881 ggagacacgt ccatgtaatc aaacatgtgt ctttcgtatg cctgtgtctc ttcacggtaa
     2941 cgaccggaaa cgttcttgct gatgaagtgt ccttcttcgt ccagagcctc attcgcctgt
     3001 gctacatggt aattatcctc ttcgtccgca gtcatatata ctacttcatc tgtgacacgc
     3061 gggttcttag gatctgtttt atcgatttta cgatatggtg cttcgatgaa accatattcg
     3121 ttgatctttg cataagatgc cagtgagttg atcagaccga tgttcggtcc ttcaggcgtc
     3181 tcgataggac acattcttcc gtagtgggaa tagtgtacat ctcgaacctc gaatccggct
     3241 ctgtctcgtg acagaccgcc aggacccagg gcggataaac gtctcttgtg tgtcagctca
     3301 cccagtgggt tgttctgatc catgaactgg gacagctggg aagatccgaa gaattctttc
     3361 acggctgctg tcaccggttt gatgttgatc agagactgtg gagaaatgcc ttccagatcc
     3421 tgtgttgtca ttctttcacg aacgactctc tccagtctgg aaagaccgat acggtactgg
     3481 ttctgcagca gttctccgac tgcacggata cgacggttgc ccaagtggtc gatatcgtca
     3541 tcatggccga tgccgtactc cagatgcatg ttatagttaa tagaagcaaa aatatcttct
     3601 tttgtgatat gcttcggaat cagctcatgg atatttttgt ggatcgcatc cttaatatct
     3661 tcttcgctgg cgttttcttc cagtattttt tgcagtaccg gataatatac taattctgta
     3721 acacctgcag attccggatc aaagtcaacc cattcgccaa ggtctaccat catattggag
     3781 agaaccttta cgttacgctg ctctgtctgg atccatacaa acggaacgcc tgcattctgg
     3841 atcttgtttg caagtgcttt atctacttct gtaccagcct cagccaggat ctcaccggta
     3901 gactcgtcta caacatcctc agccagaacc tgtccgcaga tgcggttgcg aagcagcagt
     3961 tttttgttga acttataacg tccgactttt gcaaggtcat atcttctcgg atcgaagaac
     4021 atgcttgtga tcaggctctc tgcactgtca actgccagcg gttcgcccgg acggatcttt
     4081 ttgtacagct cgagcagacc ttcctgatag ctctcggaag tatcttttgt caggctggca
     4141 aggattttcg gttcttcacc gaacagatcg atgatctccg cattggttcc gtagccaagt
     4201 gcacggatca gaacggtgat cggcaccttt ctggttctgt ctacacgaac atagaaaatg
     4261 tcattggagt ctgtctcgta ttccagccat gcaccacggt tcggaataac ggtagaagaa
     4321 tacagttttt tacccagttt atcatgtgcg attgcataat agatgccagg agaacgtacc
     4381 agctggctga cgattactcg ctctgcaccg ttgatcacga aggtaccggt tgctgtcatc
     4441 aatggcagat ctcccataaa gatctcatgc tcattgattt catcgttctc tttattatga
     4501 agtcttactt ttactttcaa annnnnnnnn nttttgtgtc acgttcttta cattcttcgt
     4561 ttgaatattt tacgtcatct tcgcacagcg tgaaatccac aaattccagg ctcagcttgc
     4621 cgctgtagtc tgtaattggc gagatatcat caaaaacctc tttgagtcca gattccagga
     4681 accatttata agagtccacc tgtatttcga tcaggttagg catctcaaga acctctttct
     4741 gtctggaata actcatacgg aagcttttgc cgcttttaat tggacgtatt ctgtttttct
     4801 ccattgacat ttcacccctg ttctttctat ctaaaaaatt caagtcactt ttggcacttt
     4861 tgggcatagt tataccaaaa agcatacact ttcacaatag tgcactttcc atactaccac
     4921 gtctgtttgt caatgtcaag tatctttttt attgacgcaa aaaccggcca ggattactcc
     4981 tgaccggtgc aagttctgtt ttacaaaaaa gcagttacaa ttatttaaga gtaacttttg
     5041 ctccttctgc ttccagtttt gttttgatgt cttcagcttc tgctttagat gcagcttctt
     5101 tcagaacctt aggagctccg tcaactactg ctttcgcttc tttcagtccc agaccagtta
     5161 cttcacgaac aactttgata actttaactt tgttagcacc aacttctgtc agttctacgt
     5221 caaactcatc tttttcttca gctgctgctt caccagcacc tgctgctgca actacaacac
     5281 ctgctgctgc ggatacacca aattcttctt caca
//
LOCUS       DAWDWH010000140         5243 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6532_length_5243_cov_10.058982, whole genome shotgun sequence.
ACCESSION   DAWDWH010000140 DAWDWH010000000
VERSION     DAWDWH010000140.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5243)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6532_length_5243_cov_10.058982"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1445 a         1142 c         1420 g         1236 t
ORIGIN      
        1 ggaaaatctg gatgttcgta ccattacgct cggtatcagc ctgctggatt gtgtcgatgc
       61 agacctggaa gtattaaatg aaaatattta ccgcaagatc acaaccgttg cgaaggatct
      121 ggtagcaacc ggtcagcaga ttgaaagaga atatgggatc ccgatcgtca acaagcgaat
      181 ctccatcaca ccgatcgctt tcgttggcgg taaggcatgc aaatgtcccg aagattatgt
      241 gagcattgca aaaacactgg accgtgccgc aaaagaggtt ggcgtcaact ttatcggcgg
      301 ctactccgca ctggtatcaa agggcatgac aaagagcgat gagaatctga ttcgctccat
      361 tccggaagca ctctctgtta cagagcgtgt ctgcagttcc gtcaacgtcg gctccacaaa
      421 atgcggtatc aacatggatg cggtacgcct gatgggcaag atcgtaaaac aggcagcaga
      481 atatacaaga gagcaggatt ctcttggctg tgcaaagctg gttgtattct gcaatgcacc
      541 ggatgacaac ccgtttatgg caggagcatt ccacggcgtg accgaggcag atgcgatcat
      601 caacgttggt gtcagcggtc ctggcgttgt aaagactgcc ctggagcagg tacgcggtga
      661 gagttttgaa gtattgtgcg agacgatcaa aaaaacggcg ttcaaggtta cgcgagtagg
      721 ccagctggtt gcacaggaag cgtccagaag acttggtatt ccgtttggca tcatcgatct
      781 ttctctggca ccgactccgg cgatcggaga cagcgttgca gatattctct gcgagatcgg
      841 tctggaacat gcaggagcac ctggcacgac ggcagcactg gcacttctga acgatcaggt
      901 gaaaaagggt ggtgtcatgg catcttctta tgtcggcggc ttaagcggag cctttatccc
      961 ggtcagtgaa gaccagggta tgatcaatgc ggtagaagcc ggtgcactga cactggaaaa
     1021 actggaagca atgacctgtg tatgctccgt cggtcttgac atgatcgcca ttccgggcaa
     1081 gacaagcgaa gcgaccatct ccggtattat cgctgatgag atggcgatcg gtatggtcaa
     1141 ccagaaaaca acggcagtgc gtgtgattcc ggtcatcgga aaagatgttg gagatatggt
     1201 agaatttggc ggactgctcg gatatgctcc ggttatgccg gtcaattcct tctcctgtga
     1261 agcatttgta tcccgcaagg gacgtatccc ggcaccgatc cacagcttca agaactaaac
     1321 cggaaagcac aaggtggagt tatggcaaag gatatgacaa aaggaaatcc ggggaagaca
     1381 ctgtttttct ttgcagtgcc gatggtactc ggaaatcttt ttcagcagct ttataatatt
     1441 attgattcta tcattgtggg aaattatgtg ggagcagatg cactggcagc cgtcggtgca
     1501 tcttcttcga ttacgtttct ctttgtggcg atcgccacgg gactgagcat cggctcttcg
     1561 gtcgtgatct cccagtattt tggggcaaaa cggtatggag aaatgaaaac agcgatccag
     1621 acggtgcttt tggcctcttt tgtgatcgca ctgtgcctga tgctggtcgg gattttcggg
     1681 acggacgcga tcctgcggtt tatgcagacc ccgcagaaaa tcttttcaga tgcatctctt
     1741 tatctgaaaa tttatttctg cggccttgtc tttcttttcc tctacaacat gctgacggca
     1801 tcgttcaatg cgatcgggga ttcaaagtcc cccttggtat ttctggcact gtcatccgtc
     1861 tgcaacatcg tactggatct gtattttgtg acaaaactga agatgggcgt agccggagct
     1921 gcactggcca ctgatatttc tcaggcaatt tcagttgttg tttcgtttct gtggctttgt
     1981 acccgcatga agaaaatgaa aacacagcaa aagagcagaa tttttgatct gcatatcctg
     2041 gagattgtct gcaatgtggc agttccgtcc atgctccagc agtcgatggt gtcaatcggg
     2101 atttttctgg tacagcgact ggtcaatggt tacggggaag ccgtgatggc aggctatgca
     2161 gcggcaacga agattgacag cattgcaatc ctgccgatgg taaacgtggg caatgccgtc
     2221 tcaacgttta ccgctcagaa catcggcggt aagaaaccgg agcgggtaaa agaaggattt
     2281 cgtgccggac ttctgatgag tgccgtgatc ggtgtgatcg tgacagggat ccttcttgta
     2341 tatgcaaagg tgtttgtcgg gctgttcatg gacagcagtt ccaatcaggc ggcgattgcc
     2401 accggcatcg aatacctgcg tgtggtcggc ctgttctatg cactgatggg tatcatgaat
     2461 acctgtaccg gcgtagtgcg gggagcagga gacatcctct ggtttttaat ggtaacgctg
     2521 atcaacttaa gctcccgtgt gatccttgct tacagcatgt ccgggatgct cggtgcaaag
     2581 gctatctggt ggtcgatccc gattggttgg ggcatcggct ttgcaatcgg ttttgtacgc
     2641 tacttaagcg gaaaatggca gaaaagtagc ctgatcgagt gagcaaaaag cagaatatca
     2701 tatgattcgt gatactgcac agttaaaagg tacatgatag atgcaagaat atatcgcgtt
     2761 taaatacgga taatctgcgt gcggtattca ttgtaaccgc cgccggggtc ggtgatgtga
     2821 tagaggtcgc tggcacgcac atttaacaga aattcatcca gttcggatgg taaaagaagc
     2881 tttttcgcat ctgcggcttt tgtcagcagt gggatggagc ttcttttttt gatctccctg
     2941 aaaagcggca atgcactctg gcgaaagccg agaactcttg cataagaaag accggcaaaa
     3001 cgatcttccg gccgttcttt ttttcgaata ccaagcagaa tatgcagcag actgcggcgg
     3061 atgcgtgcct cggtgacctg ttttgttttt aacaggctta caaacgaaga aaaagacttg
     3121 tactgtggca gcagtttgcg gatgcggccc gagaggtctg catttacgtc cagaaaagca
     3181 gtataatcct tctgcatcag aagagcatag tgtagaaggt ctgagtaatc atcaatagaa
     3241 tgaagcatct gacctgaaac agcatgttca tataattccc ggcagacatc cggcatatat
     3301 ccgcaggcaa gagccggatt tttgcggatc gctgtggcgg aagcaaaatt tccctggggc
     3361 agagaactgt gatacgaagc cccctgacgt ttgaccggca gaaaatcaaa ggaagccttc
     3421 agacgtctgc aggcaatcag gtattcgagc ccgagcgtat tattcgggaa agaaagaagc
     3481 tcggtggctg ttttgaaaga gaaaggattg cccggaggac tcaaaggatc agcgtcagaa
     3541 gcaaaatgtg agtcgtatcc gggagagctt tgagaagagt ccggaaagaa acaggattca
     3601 agggccagaa gccgtgcctt cggatatgaa tttccggcgg caagatacga tctgagatat
     3661 cgcctgaaag cttccggttc gtctgcaaga agagaagaaa gagccgaaaa agcagccgga
     3721 tcttccgttt cacatccaaa acagagtgta tcggttacat tcagggaggc aagaagtgag
     3781 caggcacccc tcgcaaaata ttcggcactg ccggctgcat agcggaccgg aagctcgagt
     3841 acgaggtctg ctccgccaag cagagccatc ctggtgcggg aaaatttatc aagaacggca
     3901 ggttcgcctc tctggacaaa atctccggac atcacgatca gggcaaagtc tgcactggta
     3961 cggcgtttcg cctccgcgat ttgccaggca tggccgttgt ggaaagggtt gtattcggca
     4021 ataatgctga tggttttcat ggtaacatcg ctccttggac ttatgattgc taactatagc
     4081 atgaagcagt acagaggtaa agagataaaa aacaatggta aaacaatatt tgtgaaaatt
     4141 ttatggcaaa gagggggagg atcagggttt gtttgtaaaa agatacatat ttctggttga
     4201 aaataccaca atcaggcgat ataatgaaag caaacgaagt ctgtaacgaa acggacagtc
     4261 aggcacagtg aaattacaga ctgtgaatgg aaatgaatta tactgaaagg agtcagaaat
     4321 catgggattt attgacgtaa tcaaagaaag agcaaaagca gcaaacaaaa cgatcgtatt
     4381 accggaaacc gaggatcgca gaacatatga agcagcagca cagatcctga aagagggaat
     4441 cgcaaagatc attctggtcg gaagcgaaga agcagtgaaa aaaggaagtg aaggactgga
     4501 tctgacagga gccgtagttg ttgacccggc tacttccgac agaacacagg cttacatcga
     4561 caagctggtt gagctgcgtc agaaaaaagg catgacaccg gaacaggcaa aagaaatcct
     4621 gttaaaccag tatctgtact atggcgtaat gatggtaaaa atgggcgatg cagacggtat
     4681 ggtatccgga gcatgccatt ctaccgctga tacactgcgt ccatgtctgc agatcctgaa
     4741 aacaaaacca ggtacaaaac tggtatccgc attcttcctg atggtcgttc cgaattgcga
     4801 atacggtgca gacggtacct ttatctttgc agattccggt ctgaaccaga acccgaatcc
     4861 ggaagaactg gctgcaatcg caggatcttc tgcagattct ttcgaactgc tggtagagaa
     4921 agagccgatc gtagcaatgc tttctcattc tacaaaaggc agtgcaaaac atgcagatgt
     4981 tgataaagtc gtagaagcta ccagacttgc aaaagagcag tatccggatc tgaaactgga
     5041 cggagagttc cagctggatg cagcaatcgt tccgagcgta ggagcttcca aagcaccggg
     5101 cagcgacatc gcaggcaaag caaacgtact gatcttcccg gatctggatg caggaaacat
     5161 cggatacaaa ctgacacaga gactggcaaa agcagaagct tacggaccaa tcacacaggg
     5221 tatcgcagca ccggtcaatg acc
//
LOCUS       DAWDWH010000141         5230 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6549_length_5230_cov_8.673623, whole genome shotgun sequence.
ACCESSION   DAWDWH010000141 DAWDWH010000000
VERSION     DAWDWH010000141.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6549_length_5230_cov_8.673623"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1605 a          963 c         1345 g         1317 t
ORIGIN      
        1 gaagaagact ccgggaagaa aagcagcagt aaaagaagag ttcgttctgg aatttgccgg
       61 caagcagatc acacaggaag aaatcaagaa aaaagtaaaa gaaaactgga caaaagttca
      121 gaaaaataaa atcaaagata tcaagacgat caaagtattc ctgcagccgg aaaatagctg
      181 tgcatactat acgatcaatg acgaaggaaa cgacagctac aaaatcgagc tttaagatca
      241 aattgtcata agatgctata ctttgaaaca ctttccagtt taaaaccgga tgaaatccac
      301 atactaggat cgcaatagaa aattgcacat ctgaaaaaga aaaggaaaac aaaacatttc
      361 aaaggaggca tgtaattatg gcaaatcgtt catctaatgt ggcggcagta ccggaagcaa
      421 aaggtgctct tgacaggttc aaaatggaag tggcaagcga acttggcgtt cctctgacgg
      481 atggttacaa tggtaacctg acatcaaaac agaacggatc tgtaggtggc tatatggtca
      541 aaaaaatgat cgaagcacag gagcgacaga tgtccggagg agccggaagc ggcatgaacg
      601 gaaacggcag tgcacagtac tgatcgggaa cttctgaagt tttggattga aaaatgacaa
      661 aagcgggtga gagaaaactt ctcttgcctg ctttttttaa aattaagaat atggtgacaa
      721 aatactttct tttttgcagg agtctgtgct ataatgacca tagtctgcga aaatacagtc
      781 agcagaactc tataatgatc tgaggtagat agagaatgaa cataggatta attgcacaca
      841 atgcaaagaa aaatctgatc gaggattttt gcgttgccta caaacgtatc ctggaaaaac
      901 atgagttgta tgcgaccggg atcacaggaa ggcgtataga ggaggcaacg aaccttcatg
      961 tccacaaatt cctggcagga agcgtgggcg gagataaaca gtttgtagaa atgatcgaac
     1021 gaaacggatt ggatatggtg atttttttct acagtccatt atttaccaat tcgagagagc
     1081 cgaatattga aatgatttca aaagcatgtg atatttataa cataccgatt gctaccaata
     1141 tcgcaacagc agaatctctg gtcatcggtc tgcaaaaagg tgatctggag tggagggaaa
     1201 atatatgacg atcacagacg ttgggaatct tttcatgttt gccggaggcc ttggtatgtt
     1261 cctctacggt atgcacagta tgtccggagg cattcaaaaa acagccggca ataaaatgaa
     1321 agagctgctc gggattctga cgagtaatca ctttatggca gttgtggtcg gggcacttgt
     1381 cacggcgatc atccagagca gcggtgccac aacggttatg gtggtcggat ttgtcaatgc
     1441 gggcatcatg acactgacac aggccgttgg cgtcattatg ggagcaaaca ttggtacctg
     1501 tattacggcg tggatcgtat cacttggaca gctgggagat gctttcaagg cattttcccc
     1561 ttctctgtat gcaccacttc tggtcgggat cggagccttt ctgatcatgt tcagcaaaaa
     1621 ggcgaaaaag cagacggtcg gtgagatcct ggtaggactt ggccttttgt ttatcggtct
     1681 ggattttatg ggagatgctg cgggagatta cctgcatctt ccaatcttta ccagggcatt
     1741 tgagctcttt ggcagcaatc cgatccttgg tatcctgatc ggtgtgatcg ttacggcgat
     1801 catgcagagt tcatcggctg cagttggtgt ccttcagaca cttgcagcgg caggtggtgt
     1861 tgtgactgca agttctgcca tgtatatcag ccttgggtcg aatatcggtt cctgctgtac
     1921 ggcacttttg tccagccttg gcgagtcacg caatgcaaaa agatcagctg tgatccatct
     1981 cacgttcaat gtactcggtg cactggtctg gggcagcgga ttgtttgtcc tgtttcagtt
     2041 ccgcccggct ttcgggacaa tggggatcga cagtgtcggt atctcggtat tccatacgat
     2101 ctttaacctt gtctgtacgg ttcttatgac accgctgagg gggaaactgg ttgcattatc
     2161 cggtcttctg gtcaaaggca gcgatgaaca ggaacaggca gagcagagtg acgatgcgat
     2221 cacactgcgt catctggatg accgtatcct ggagacaccg tcttttgccg ttgagaatgc
     2281 gattctggaa gttgtacaca tggggcacat tgcaaacgaa aatatggaac gtgcctttga
     2341 agccgttatg aattatgaca aagaaaaaat cgagcaggtt taccgtacgg aaaaagtcat
     2401 caataagatg gaaaaactga tcacagagta cctggttaag atcagcaatc tttctttgaa
     2461 tgaagaccag cacatggttg tcaacgatct gttttattcg gtcagtgaca tcgagctgat
     2521 ggatgtcaaa gaggatggcg aaccgatcac tttttccgaa gaaggcagat gtgatctggc
     2581 agagatcatg gatctggttg aaaaatcttt tgtattttcc atatcggcca gagaaaaggt
     2641 cagcatggat gatgcaaata aagttgtaaa atatgaggat atggtcgata atctggaaga
     2701 agagctgagg gagaagcata ttgaacgcct ttccagacag ttgtgcaatc cgacaagcgg
     2761 agtggcattt ctggatatta tcagcaatct ggagcgtatc tctgaccatg cctacaatct
     2821 ggcaggctat gtgatgtcag aacaataaaa aggaatataa aggagcagtc atgggaaaat
     2881 ggatctatta cgtggaagac gatacaagta taagggagtt ggtgctgtac gcactgaaaa
     2941 ccgcagaatt tcaggtgatg ggatttgaaa atgctgcttc tttttataaa agaatgaaag
     3001 aacagcagcc ggatctgatt ctgctggata ttatgcttcc ggatgaggat ggagtcagca
     3061 ttctgaaaaa actgaaaagc agaccggata cggagaatat cccggtcatt atgatgacag
     3121 caaaatcttc ggaatacgat aaagtactgg gtctggattc cggtgcggat gattatatca
     3181 caaaaccgtt tggagttatg gaactgatct ccagagtaaa ggcggtgatc cgcagaagtg
     3241 atcggagcaa aggaagtgcg ggagaagtct taaagatcgg agagctggtt ctggatgaac
     3301 agaagcatga ggtttacgcc cgcggacagg aggtcagtct tacgttcaaa gagtttgaac
     3361 ttttgtctta tcttatgaaa aaccgaggac tggtgctctc tcgtgataag atccttaaca
     3421 ccatatggaa ttatgagtat gagggagaga gccgcaccgt agacgtacat attggttccc
     3481 tgcgacagaa acttgggaca tgcggagatt ttataaagac aatacgcgga atcggttata
     3541 aaatagagga ttaagcgatg aaacaacgga tctatcgaaa ctatttctgg gttgcactta
     3601 caacgatact gattgtatcg tttgtttttg tattttctta taatcaaacg attcatgatc
     3661 agaaagagga agggatgcgg gaagaggcag aattcatttc tgcacttctg gaaaagcaag
     3721 gagatgcaga tgtcggtttt caggcggcag ccggaaagac agactttcgc atcaccctga
     3781 tcgctgaaaa tggtatggtg ttgtatgaca ataaagtcaa agatgtatcg ggcatggaaa
     3841 atcatgcaaa acggccggaa atcttaaaag ccagcaagaa cggtgaaggt tccagcagcc
     3901 gtatttccag tacgatccag tcggagactt attattatgc acgaaaactt tccgacggca
     3961 gatatctgcg tctttcaagt gagggactga gtatctggag cgggtttgta caggtacttc
     4021 cggcacttac cggaatcgca ctgtttgtca tatttatcac atttctgatc gcccatttcc
     4081 ttaccagaaa tatcgtagaa gcgatcaacg gcattgatct ggaaaatccg atggagaagg
     4141 ttgtatttcc ggagcttggc gttttgcaga aacggcttgg ttttcagaat cgaaaaatcc
     4201 agacgcagat gcaaaatatg aaagaacagg agtataaatt aactgcaatt acggaaaata
     4261 tgaatgaagg gctcattatg ctggatgcag accgcaagat tcagtatatg aaccagagct
     4321 gccaggatct gtttgaagta tccggaagca aatttatcgg gcagactatt tccggattct
     4381 gtgacagcat tccgatgcag gaggtagtag aatctgcact gaaaggaaat gttcatactg
     4441 ccatgcagga actggatcat aaaaaagttc agtattttgg aaatccaatc ctggaaaatc
     4501 aaaaaattca gggaattatc attctggtcc tggatattac tgaacgggag cggacagagc
     4561 ggatgcgaaa agaattttcg gcaaatgttt cacatgaact gaagacaccg ctgacttcca
     4621 tctctggttt tgccgagctg atggagaatc atctggttgc accggaagat gtgccggagt
     4681 ttgccgccaa aatacacagg gaatccgaga gactgctgac gcttgtaaat gatattatca
     4741 aagtcagtca gctggatgac aaggaagtat attatggaaa agagaatata gatctgctgt
     4801 cttttgcaaa agaggtctgt gaccgcctgg aaccgatggc acagagccgg aacgttacgg
     4861 ttgaactttc cggagatgct gttttgtatc tcgcgtccag gcagctgatg aatgatctgt
     4921 tttataatct gatcgaaaat ggaatcaaat acaacaagcc aaacggaaaa ctgtgggtta
     4981 cggtcagcga aaagaaagga catccgtgta tcagtgtgaa ggataccggg atcggtgtgc
     5041 cgatgaaata tcagagccgg atttttgaac ggttttttcg tgtggataaa agtcattcaa
     5101 aacagacggg cggaacaggt cttggactgt ccattgtcaa acatgtggtg gaatattatg
     5161 gcggttacat tgaaatccac agcaaaatgg aagaaggaac agaaatcgtt gtacatctgt
     5221 aaatgaatga
//
LOCUS       DAWDWH010000142         5204 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6575_length_5204_cov_10.208390, whole genome shotgun sequence.
ACCESSION   DAWDWH010000142 DAWDWH010000000
VERSION     DAWDWH010000142.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6575_length_5204_cov_10.208390"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1452..1461
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1270 a         1310 c          988 g         1626 t
ORIGIN      
        1 aggcaaaaga acttggtttt acgaaaaagg gctgttttgt gatttctttt aacggcggtg
       61 aaatctacga tatgtaccgc aaaaagagta ttttcaaaaa accgcttgca atgccgctcg
      121 ttcgccgcat ttttgacgaa gcccttcagt gcggtcttca ctgccagacc tattccgaca
      181 gtgagatcgt tacagagcac gataccgaag aggtaaaacg ctactcttcc atcgtcaaga
      241 ttccatacaa agtcgttccg gatgtgaccg cttttctgaa atcagaaccg gtcaaagtgc
      301 tggttgtaaa ttatgaaaac aaggagcatc tccgtgatta tcttgaaaag accgctgact
      361 ttgcagacgg caagatcaac cgtttcttct ccagcaatga atacctggag cacgtcgctc
      421 ccggtattga caagggctct gccgttcgtt ttctctgtga atacaccgga atcccactgt
      481 gtaataccat tgctgccgga gatgccgaaa atgatatttc catgatccgg gccgcccata
      541 tcggctgtgc tatgagaaat gccgaagaag aagtgaagca ggctgctgac tatatcacag
      601 aaaatgacaa caaccacgac ggcgttgccg agatcatcca taaatttgta ttaaaataag
      661 atatccggat aagtacctct gcaatccgcc gcttaaaaag gagatgtgcc tttcactgca
      721 catctcccat ttttatacga gtttttccag ttctgtaaga tattgttcaa acaccttcaa
      781 tgcctcttct accggttctt tcttttccat atcaacacca cagagtttca gcaggtcgat
      841 cggatccata gaacagccac cgcttaaaaa ggctttatat ttctcaacaa tcccttcttc
      901 ctttgcaagg atcttggtgc tgatcgcaat ggccgctgaa aatccggtcg catattgata
      961 aacataaaac ggtgtgtaaa aatgtggaat ccttgcccac tcatagcgga tcagatcatc
     1021 cgaaaccatt gcttctccat agtatttctg atttaatgca tagtaagcat cgcacagcac
     1081 atccgctgtc agtgtttcgc cctgttccac tttctgatgc atttcctgct caaaatgtgc
     1141 aaacatggtc tgacgataca gagttgtgcg aaactgctcc agaaaataat tgatcagata
     1201 tgccttttct tttttatctt ctgtcttttc catcagatca tggatcagca gtgcctcgtt
     1261 acaggtagat gccacttctg caacaaatat gcgatatccg gcattcagat aattctgggc
     1321 atgatcggaa taccaggaat gcagagcgtg ccccatttca tgggccagcg taaatacatg
     1381 gtttaaattt ccctgatagt tcagaaggac atacggatgc gttccataag ctccccagga
     1441 ataggctcct gnnnnnnnnn ntggtgctgg cggtgctgct gctggccctg ggctatatga
     1501 agctgttcta cgcccagcag cctttttctc agttccagat aacgctgcat cggctgctga
     1561 tgttcttcta cggtgcgaat caaattagta taaacactga ttggaatatt tccgccatcc
     1621 agtgctgctt ccagagaaga actgtatttg cgttcttttg caaaaaaagc tgtctgacgc
     1681 acatttgcat caaaaacggc cgcaatcgta tttgcaaacc ctttgtagcc gctatacagg
     1741 tttgtaaacg cttcttttcg gatctctctg tttttatttt ccagataatg tacatagcgt
     1801 tcctgcgtca gcggctgctc atttccattt tcatctttta ccggctgaaa ggtaatatct
     1861 gcattattaa acataccgta aatattagaa ggggcacggc caagttcact tacccttgcc
     1921 agaagttctt ctttctcttc actgagactg tgctcttttg ttcgcaggat ttcttccaaa
     1981 aatcgtcgat attccgagaa aggctcatcc gccagatact ccgttaattt ttcatccgga
     2041 atcttcaaaa tctccggctc cagaaaggcg gacacttcat tgacctgatt catcagcacc
     2101 tgtaccttcc cggaacattt ctggctttcg ccatttgcca tatcctgatg cagaagctga
     2161 tttgcatata catatgcttt ttcaaatctc tgattcagtt cactgtaaaa attcagtgcc
     2221 tttttcagat tttctgcacc ttccacaagc gttcctttgc aggaatacag ctcctgtgcc
     2281 agttttcgga ttttttcaag atcttcactc catgctgcct gatcctgata taaatcttcc
     2341 agtgcccagg taaattcttt tgaaatttct tctcgttttt ttacatgttc cattttacgc
     2401 ctcctgcagt tttgcgacca ctactttact gttctgccgt tcattcagca ctgccgtctt
     2461 cagtatcgta agattattct gtctgcggca catttcttca agtagtgttc cctgacgatt
     2521 atacagcaca agaacagctc ctctctttaa aagcgtttct gcttttttaa aaaatgtcct
     2581 atagaatgct tcatctgcgg aagccggcat atctgtaatc agttcatcaa acagatagtc
     2641 atgttcaaag gtaaagaagt ctctggcaat ataatggatc ggcattctcg tcaccgcagt
     2701 attgtctctt gctttctgaa tcccttccgc atagatatca acaccataca acggatcggc
     2761 agaacgaaaa tacttgcgtt cgatcagcat ggttccgaca ccgcagaacg gatcgagtac
     2821 ctgtgcattt tctttcagat ggtctccggc gatctgcatg atcagtgctg cattcaccgg
     2881 ggcaatggaa gcggcaatgc tctcttttcg gtaagcaaaa cgttcatctt ccatcgtata
     2941 cagcctcagg aacggcagat aaccaccttt tttgctctcg acaatgcgga tttcaatttc
     3001 ataatccgaa atggaattga tcagcatccg ttcgcttttt tcttccagca aagatgccgt
     3061 ttttttcaca aattcgcttt ttttgcgttc atccagagaa cctttgtact cgatgcgaaa
     3121 cagaaacgct cctttttcct catgcagact ttgaagcagt tccatgatct cggtgttcca
     3181 taccgtctgt gccgccgcct ccgccgtata ctcccgttcc acttttccgt gaagcggaaa
     3241 taggagctcc tgccaggttc gtattttcgc gatttcctgc agatctgccc cctgaatgcg
     3301 aacgcctccg gcaagcatgg agacttcacc ggtctggatc tgtcttgctg tgatctggcg
     3361 gttattccga ttgcacaaaa ggatcacatc ttccgtcttt tccccacaga atgtatgatg
     3421 ttttggcttt tcatatttta agatcatgcg ggaaagaacg gctgcttctt tctggagatg
     3481 ttttttcccc tcgtctgccg catagagcag ttcttcctgc aatgttttct gtcttttctt
     3541 taatttggga agcatctgtg aaaaattcag tttttccatc gcttttaaat aagactctcg
     3601 gacaaagagc tgtttttctt tctcataggc ttcgtagagc cacggaagaa catcttcgga
     3661 ttccaattct ccgaggatca gtgctgcatt tttccgtatc ttggcgtctt tatcctttaa
     3721 tagagctgcc agttttcgaa aatcgcctgc cagcatataa gcaaatgcac ttttttcttt
     3781 cttatctgct atcagctgct ttaaacagat cagatttgcc cggacatctt ctcccctgca
     3841 aatactttca tatatatctt taaccatacc tttcctttct atacctgtct tctcttcctg
     3901 ttcttataat ggctgcatat ttctactgtt cagatttttg gattaatttc cattttcttc
     3961 aatatacttt gccacatctt ctttaaatgc attccatgca tcttcatgct ccacaaaata
     4021 ctttggacag tcttttccgg taacatcata atggcggatc acattgtcca tcgtcagatt
     4081 aaactttccg cacagaaatg caaccagctc cacaagagac tgatacgtca cctcctggaa
     4141 cgctcctgac tcatccggat gacaacattc aatggacact gtatcgttgt tgcggttgtt
     4201 agatgcatag gcgatctcat ttgtcggaat gcactgaatg atctctccgt ccagtccgat
     4261 gataaaattg ctgctgacct gattttctcc ggtgtccttg aggctttcaa aataatcccg
     4321 attattctgt gcggtacttc ctggattagc cgtataatga accaccacac cacgcactcc
     4381 atcggttgca atccctggtc tggagtattc gttgacagtc agaagctgca catcaagctc
     4441 cggctgtgga acctgtttta caggatctcc gatatcatac tgctcccatg catccatccc
     4501 tgccttctgg tatgtcattt ttttcgtacc ggtgttcctg tgaaagaaaa agacaaaaag
     4561 cagtaccaga acaactgctg tcaaaatcac cgatacggtg attctttttt gaatttgcct
     4621 tttcctctct gccatttttc ttcggcttct gtcgttctcc cacatatctg cctccaatgt
     4681 aaaatgcgat aattgcagtc tgctttcact cccgccggcg tgcaaaaaag aaaggcggat
     4741 tcctttttcg ggaaatccgc ctgttccgct tttattcatc aacgctgtag tttggtgcct
     4801 cttttgtgat atgaatatca tgcggatgag attctttcag agatgctgca gaaatcttga
     4861 taaatcttcc gttctctttc agatattcaa tatcttttgc tccgcagtaa cccataccgg
     4921 aacgaagacc gcccatcagc tggaatacgg tatcttctac gcttccctta taagctacac
     4981 gtccttcaac accttccggt accagcttct tcgcatcaga ctggaagtaa cggtctttgc
     5041 ttccgttttc cattgctgcg atagatccca taccacggta tactttatat tttcttccct
     5101 ggtacagttc aaatgttccc gggctctcat cacatccagc aaacatgctt cccatcatac
     5161 aaacgtttgc accggctgcg atcgcttttg tcatatctcc ggag
//
LOCUS       DAWDWH010000143         5199 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6581_length_5199_cov_11.189930, whole genome shotgun sequence.
ACCESSION   DAWDWH010000143 DAWDWH010000000
VERSION     DAWDWH010000143.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6581_length_5199_cov_11.189930"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1215 a         1285 c          859 g         1840 t
ORIGIN      
        1 acgatgaccg taccatttaa cttcttttct tcagacatga ttaactctcc tattgtttac
       61 tctctctttt aagaatgttt cgattttatt taagaaccac ttggcacata ccgagaacac
      121 aaccgaaccg cagatgaatc cacggttctt tcttatagac atccacggta tcatcacatt
      181 cattttttac aaacctgtaa cccataccac acatgatatc tctagctttt tttcggtttt
      241 cacggtcgat cagaaaatcc agatctccca tctgacggta ttccggtctg ggatagaatc
      301 ccttcatgat ccagcctttc atgggaagta tgcgtatttc tccctgttct gtgaaggctc
      361 tgaaaatttt gtttccttca cttctctgta ccgcatccat cacggtacag gactgactgg
      421 ttttctgcca ttttttcatg atatctgcat cttccggctc tgcgatctgc ctgagtgcat
      481 cgtatgccat gcagataaga ttgttatttt ttgccgtcag gaacagctgt ttcatcgaaa
      541 ctttttccgg ctttggctgt ggtttccgct cttccaggac agagcggatc aactcgatga
      601 gatatttggc tgcaagaatt ttttcctgtt cctgctgctc tttcttttct tttgcttcta
      661 tgtctgaagt attggatttt acgttggtct ttctgctgtt cttcatcgta tctttttcat
      721 tcaccgattc tgttcttctt tcttgtcact catcattttc catgccattt tggcagcctc
      781 atgtgaaata ttgcactgca tccggtacag cggcacacgt tctgccgtct gcttgagcag
      841 agtgatcgtc cggatcagtt ttgtctgatc tgccggccgg tagacctggc gaaataaata
      901 cggaaacgca tctgccggtg aaatcatctc gatatggttc ttttctccgc gttccagcag
      961 acagatccct ttgaccggta ctttctggtt gctgtccagg ttatgtttgc cattccacgg
     1021 tgtgccgcag acatagatgg catcctctcg cacctgaatc agtggcttgt cgtcatttac
     1081 catcaccgca cgttcaccaa aaacttcacg ccacagccgt gtgtgggttg attttccggt
     1141 gccactcgga gctgtaaaca gatacgcctg accgtctagt gcaactgcgg aacagtgaaa
     1201 caaaaacgta tcgtgttcaa ccataatttc cgaaatctta cggtacaccg ccagactctc
     1261 cagcattcca ttcgaactgc tgacgccaga ctgatcctct ctttcgatct cctcatcact
     1321 ggctgcgatc agtatccccg gctcttccac ttcataactt ctgcactgct tctccagata
     1381 tggatactgc atctgtaatg taaaagcaat atctgcaaac cggtagttct tcgttaccat
     1441 tttctactcc tccaacaatc cctttgtgcg aagtttttcc agaaacttct tcacatcggc
     1501 tgtcgctttc tcttctttca catcgtattc cgatgtcagt gcttttacaa gatctgcctc
     1561 tgttgttccg ccctgaagtt tctcccacag aaacgctccc acttcattca gcctgatcag
     1621 ccctgccagt cctccgtcat ttactgtaat cggcacagcc atcttctcgc cgccgatcgt
     1681 atctaaaaga taatctcctt tgattttcat atcgattttc tccttattat cactctgaat
     1741 atggaaacat tatttacact gctcctatca aatcgaaata ttaagccgta aaaaccattc
     1801 tctactccat aattatagcg atatttgtcc tcctggtcaa catggcttat ttttcatttt
     1861 tattcaacat gacttatttt tatccagatt ttattttttc tttctgcatt tcaggcaaaa
     1921 acgtttcacc cttctgaaaa agacagtatt tacccaaaat cctgcccaca tcctatgctt
     1981 tatatcattc accgggatca cctcatctcc acggatgatt tctgtcaccc ttccgatcat
     2041 ctgttccctt tttatgccat attcatccga gaactgatta tccccacgga atatataagc
     2101 attttctttt ttccctgtta cccggtgcag cacaaatgta ccgttttccc gcttatacag
     2161 tatgatatca tactttttta tcctgtccgg aagtgctgca agtgtgactt tgtcccgccc
     2221 ctggacgata aacggctgca tactcgttcc tcccggataa aaagaaaact ctccacccct
     2281 tgcgagaact tttcggattg tctgttctat ttgttccagt tttatttccc gttgctgcat
     2341 atcggttgct ctcctccact gcattccaga ataatgcgga aaatccctgc ccctctatcc
     2401 ggaaaagcag agatttactg cattcatatg catttgttat cgtttttcag tcccagccgt
     2461 ccgcactgtc ttcccaggag tcttcaccac caatttccag ttcttctgtt tctacattct
     2521 cctgcgaagt taccgtgctg ttttctgccg cctgcttatc agtacctgct tctgtatttt
     2581 tctgtcctgt actgtcactt tctgttgttt tctgcggtaa attctgaatc tccgtttcct
     2641 tgtctgcttt tgtttctttt actgtttttg tttcttttac tgtctctgtc tcactttcct
     2701 gttttactgt tgccatctgc tgtgtctgat ctttcttttt gcatccggca caggaacaga
     2761 ttataagaca tatcaaacat atataactaa tctttttcat actatttcct ttctttttca
     2821 caggtttaaa aatacctgca ctccaatcca gcagtgcagg tattacatta tgctttattc
     2881 aaaatccgga cctaattcgt tttcctcagt tgtaggagta gattgattca tcacatccgc
     2941 aaattcaagt aataacactt ccatttccgg ttcttcataa tttcttttca ttttttgtca
     3001 cctctttatt atttgctttc atacggtgca acatcggaat atacggtata ggttgcatct
     3061 ccgtccgtat aagtgacata agatcttcca taccattttt cttttttgcc cgcttttaat
     3121 cgtgcttcat aagttccatt atttgcagtt ccctttgcaa ttgatttctt tgttctggtt
     3181 gcacctttta ccattgcatt cgtattattc ttatacaggt tatcccatcc tgtctgatct
     3241 gtaataataa tgccatgttc gatcactctg tatgacttat caactgaacg ttttgcaacg
     3301 aatgaaattt ttcccagaga agtaagataa cgtcctgcac agaaaatatt tacttccggt
     3361 tctgcttcaa cataagaggc ctccaatgtc atattttcaa caacgtagaa tgtataactc
     3421 gtggttgtac acataacttt tccaaaagct cccggagtct cagacggtat ctgccagctt
     3481 gcaaattttt gagtatttgc tgttcccggt gctgttacct tcactcttga aaacattgga
     3541 acataaataa cttttcctgt ggcatcactg ctttcgtatt caccatcacc atccacagca
     3601 gtcaaactaa gatcacttaa attaccttcg tctgatttta ttgttacctt atataatctt
     3661 accagatcat tcagtgccct tctcagttta gcttcttctg ctgcaactgc ctcagaatct
     3721 gtagatgtag taagtttctg agctgcttct atctctttta taagttccgt taatgacgca
     3781 tttgaataat gagggttacc ctgattattc tcaagcactt ttttatttcc ttccccaatg
     3841 agtgcttgca atgcgtctgt ggatggtgca gcatttacaa ctactgtaca ggttgcactc
     3901 tttgtattgt cttcttttga tgttgcggta atcgtagctt ccccggcact gactgctgtt
     3961 atgactccat tgtcaacagt tgcaacatcc ggtttatctg ttgtccaatc aactgttgaa
     4021 tttgttgcat tttttacagt tgctgtcagt gtctgtgtaa ctgaatcacc tgctgtcatt
     4081 gttatataag tctggtcaag actcaccgat acttctgcat cttttgcaaa caatgccaca
     4141 taagagtact gactcttgct gtttacgact tcattaagta ctgctgttga tttctcagtg
     4201 gttgctccgt cagcatctgt gatcagccat tttacaaaat ggcaaccaga agccggagtt
     4261 gctgtcatag taacagcagt atcaatctta tccaacatta aactcttatc tgtagacact
     4321 gttcctaaat ttatatcatt gctactaact gatggaacag atatcacttt ggacacatca
     4381 ctgtcatttg catcatcttc ctgccataag gttattttag atgcagtaag ataagagttg
     4441 ttatgatcgt acaccgatga tacacgtaac cgaatatggg ttgcttttac agtaaacgga
     4501 agaatcaccg tctgttcctc attattattt tcaaactgtc ctttgcaaac aaccacaaaa
     4561 tcactatcac ttggctgttc ttttgttgta gttgccgcca aaatttcata atcttttgca
     4621 ttacaggaga caacatctga tcttgatcga tatgtaagct ttctgatatt tttctctgag
     4681 ccaaatccag cttcaatcca caacttattt gttgacgacg gtatcccgcc ttctccacta
     4741 ccagtattat tatattttgt atgccaatga tgggcttcga ttccatcaaa tgcataccag
     4801 gcaggaccat catttccagt gcttgttccc tgtgctgaat tagctcgtac attatcctgc
     4861 tgtatactta tttgagaata agaaagctgt tcaataaaag ttggtgtata attggcacat
     4921 tcagccgtat ttgttaaaga aaatgaactt cttgaaacaa ctgcattatc cttcttccaa
     4981 ccattgaaaa catatccttc atttggtgcc gcatacaaag taacatttcg tgtcgttcct
     5041 gacaggcatt ttcctattat tgaagctgat tcacctgccg cagctgactc acttgcatca
     5101 tttgttgttt catatccagg cggcaggatt gttaccgttc catttttaat gcctgcactt
     5161 gtctcgatat tttccaattc tgatactctt gccgaagta
//
LOCUS       DAWDWH010000144         5189 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6593_length_5189_cov_8.246202, whole genome shotgun sequence.
ACCESSION   DAWDWH010000144 DAWDWH010000000
VERSION     DAWDWH010000144.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6593_length_5189_cov_8.246202"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4107..4116
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1766 a         1097 c         1312 g         1004 t
ORIGIN      
        1 ctatacggtg ggtggctacc agatcttact gtatgacagt gcagatcagg cagagcttgc
       61 agtgacaaac ctgaaaaaag attacccggg agtacacatt tttcaggata tcccggtcac
      121 actggaagag gcagcaagag acggcgatgc agatgtgcag gtagaaatac aggatgcagc
      181 cgaaaaagaa gataagagcg aaacagagac ggaaatccag gcatttgacg gcatccatat
      241 gatgggtatg gacaagttga aagaggatgc aaaagactgg agcggcgatg caaccgtcgc
      301 agtcatcgac agcggaatcg atgaggatca tccatggttt acaacaagac tggatcgcaa
      361 aaacagtgtc aatctggcag cagacggttt aggcaaagat gcatacaatg atccgaacca
      421 gggacacggc acacacgttg ccggcatcgt aacccaggca acaccggtgc aggtcaagat
      481 catggccgtg cgagtatttg acctgactgg ttctgcttct tacacaacga tcacacttgg
      541 cgttgactat gcggtacagc ataatgccga tgttatcaac atgagtcttg gatttgagat
      601 cctgccggga tttgaaagcg atgacaccga tttgatggat gaagcttttg cgagagctct
      661 ggaagccggc acaacggttt gtgtggcatc cggaaacgaa tataccgata ccgcaagaag
      721 ttatccggca agcagccgct ggacgatcgc agtcggatct ctggagccgg ataaagacgg
      781 aaattcttac attcgctcgg atttttccaa caacggaacc ctgcttgatt ttgcagcacc
      841 gggaagaaac atcttcagtg catggatcgg cgagggcgag acaaccgaga tcgcaagcgg
      901 aacctcaatg gcaacgccgc atctggcagc ggcagcagcc tatgtcaaac tgaaacatcc
      961 ggattacaac cagagagaag tttactccgt tttccgtgat tatgccgtag atcttggcga
     1021 gccgggcaaa gataccgagt ttggatatgg atacgtagat ttcacaaatt atgcggacga
     1081 agaccagaac acagcgaaaa aatatcaggc gatcaccgca caggcacaga tcaataaaac
     1141 catgaacgat ctggataagc catttacgat cgatacagag ctttccagag gaaacggaac
     1201 cttaagtttt gcaagcaccg atgagaaagt ggcaaaagta aacggcagtc aggttcaggt
     1261 aacgggagtc ggcagctgtg agatcgtgat caccgcatcc gaaaccgacc agtattatca
     1321 gaccgaagag agggtaaaga tccgtgtgga aaaaggacag cagaccatca acgttccggt
     1381 gaccgaatac cacaaatacc tctccgataa aaacttcaag gtaacggcaa gcatcgaaaa
     1441 accgggcgac ggcaaactgg tatttctggc aaacgaaaac gatgtgctgg aagtgaccga
     1501 agatggctat gtcaccattt taggaaccgg tacaacacag atttatgcag tggcaaaagc
     1561 aagcgataat tttgtaagac aggtcagcga tgcaatcacc gtcaccatag aggaagatcc
     1621 gaaaccgcag ccgaagccgg atgatgatca gaatcagaac ggcagcggag atcagaatca
     1681 gcagaacacg gcatccgtga aaaaagcagt gatccgcaaa gtgcagccga aaaaagcaca
     1741 ggtcaaagta acctggaaaa aacaggcagc agcaggcagc tacgagatcc gttatgcact
     1801 taagaaaaac atgaagaatg caaagacggt gaaagcaaac ggcaaaaaga gcagcattac
     1861 catcaaaaaa ctaaaatcag gtaaaaaata ttatatacag gtacgtgccg tgaagaagaa
     1921 aggcacagca gtgacaaagg gagcatggag tgcgaaaaag agcgtacgcg tgaaataatg
     1981 gcaagaaagg aagaaaaaca tgaaaaagag atttgattat caggtgctgg cagcgatctt
     2041 tgcggcagcg atgatcgtat ccggagtcgg aatgtatcag acaacggcaa aagcagccga
     2101 aggacagatc cagacaccgc ttcagaaaac agaagccggg acagaagagt ctgcaaaact
     2161 gaaagcagac accgatgccg catccgtaga tccgacggag gacggagaat acactctgac
     2221 ttttgaggca aaacaggaag gcagcgacga agaatccatg ctggcaggct attttgaccc
     2281 gaaagcaaaa ctgaccgttg agaacggaaa aatgtatgta acctttttaa ataccaagct
     2341 ttccgatttt ctccttgatt tcacagtggc atcagacgga acgtatgcac agactgagaa
     2401 gactggtttc ggtgagccgg acggaaccgg aagctacgcc atgtatgagt acaagatgga
     2461 gatcacaaag ccatcggaag tcaacaaagg agcagcactt gtctcagcga tgggcggtca
     2521 gaacagtgac attggaaatt ttgacaaata cctcaagacg gacttcactt tcctgacact
     2581 ggaaaaaggc tggagcggat acgatgcatc caaaacagaa gacaaaccga ccggggcagc
     2641 agcactcaac gaagcacttc gcgattacgg catggacaaa gacaatgacg gaaccgttac
     2701 agcagaagaa gtagctcggt atggcggaac cacactggat ttaagtgagt gcaacttaag
     2761 cgagatcgga cttctgcgtt atttgccatc tcagatcata acgttaaatc tctcaaacaa
     2821 tgagatcaca gcgattccgg aaggactgct ggataacctg accagtctgg aaaattttta
     2881 tatcgagcac aacaagatca cagaaattcc ggcaggactg tttaaaaaca accacagcct
     2941 ggactggatt tcttttaccg gcaatcagat ttcctctctg aaagataaca cctttgaagg
     3001 gctggatgct ctgacgatcc ttgatctgga aggaaaccag atccgggagg tttcccaaaa
     3061 tgccctgacc ggcatgccga aacttcagca actctccttt gtgggaaatg gactggaaga
     3121 tctgaagaat gatgtgctaa aaccgcttgc cggatctttg cggtggctgt ttttacagga
     3181 aaataacatc gagagcctgc caaagaccgt ggaagatctt ttttcccttg aggaactcaa
     3241 tgcatacgat aacggtatga aagatatcac aaaagtggat ttttcaaaac ttccacagct
     3301 tcaggaagtc aacttcatgc acaatgagat tcgtgaaatc ccgtccggaa cgtttgcgaa
     3361 gaatgaaaag ctggcagggc tggatctgta tgacaatctg ctgactacga tgtcaccgga
     3421 tacccttccg gcaacagcag ttctgagaaa actggatatc cgcctgaata acattcaggt
     3481 cgtagaccgc aaactgatca tgaaaagcca gagctttaac aaattctatc cgcaaaaaag
     3541 tgcgatgagt ctgaagattg aaaaagacgg cgaaaacggc gtgaaatggt cacaggaact
     3601 gagtattctg gatttgctgt actggttcga tgcaacgaac gatgcaaaag tcgaagaagc
     3661 acagtctgtt gatgaataca gagaatttct gaaagacaaa ggctatgaag gccgcgatat
     3721 catggatatt ttaaatgacc agtactatga ctgggagatt attaccagca tcgaaaagaa
     3781 aaacgcagac ggaacctacg aaaccgtatg gaagagtacg gatgatgata aggcagacct
     3841 ggcaggagga gcctttgcaa cagcagaaaa aggcacatac cgcgtcgtaa aagatctgta
     3901 ttccggaaac agcggactgc tgttctatcg tttcagcgta ttttcaaacg aggtcaatac
     3961 agcacagcag aacaatgtga caccgggtat agacaagccg aataatggaa gtaacagcaa
     4021 tacaaacggc ggtaacaacg gaaacagtgc aaatccggga aatacagcgg tgaaaaaacc
     4081 ggcaaaggtg aaaaaagtaa cggcaannnn nnnnnnacgc actgcagtga tcagctggaa
     4141 aaaggtaaaa ggtgcatccg gttatgaagt ttaccgttct acaaagaaaa acggaaaata
     4201 caaaaagatc aagacgatca aaaaagcagg aacggttcgt tttacagaca aaaagctgaa
     4261 aaaagggttc gcttattact ataaagtcag agcttacaga aaaacagctg ttgcgaaagt
     4321 gtatggaaac gattcggctg caaaaaaagt aaaaataaag taaacattaa agtaagatat
     4381 cacgacgcaa cagattacca aaacgaggag gaaacagaat atgaagaaaa aatttgtatg
     4441 ccagctgctt gccctttcta tgactgttgt catggcagtt ccacaggcag gactgccggt
     4501 atttgccgca tctgcagcaa atacacagca ggcacagctc aaagaagaag caaaagtcgg
     4561 aacagagatc acacagcttg aaggatcaac cagctggatg acagggaccc tggaaaaaat
     4621 cgtgctttct gagattactt ccgattctgc ttactgcaag gcgattacgg gcatcagcgt
     4681 caacggtgta gattatgtgg cagactggaa tgaagaagat gatacactgg tatacagtgc
     4741 cggctggctt ggtatgacga tctacagcgg agcattcaaa gaaggcgaaa atacgatcgt
     4801 gatcaaagct gacggttacc tagacaaaac catcctcttt acaaaagcag aaaatgagtg
     4861 cacgtttgta agccagtccg acggaaacgg tggtcaggaa ccggaagtac tggataaaac
     4921 agctctgacg gaaaaactgg aagaagcaaa agcactgata caaggcgata agagcgacga
     4981 ggaatgggaa gatctccaga gtgcgatcac agaagcagaa catacattaa gagtggcaac
     5041 cacgcaggct tccttaaata ctgcactgga agatctgaca aaagcaatgg agaaatttac
     5101 cacagggctg actgcaccga ctgttaccgc agttgcttat gatggcaacg aactgaccat
     5161 cgaagagatc aatcattatt ccgatgatg
//
LOCUS       DAWDWH010000145         5178 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6609_length_5178_cov_10.600625, whole genome shotgun sequence.
ACCESSION   DAWDWH010000145 DAWDWH010000000
VERSION     DAWDWH010000145.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6609_length_5178_cov_10.600625"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3178..3187
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3844..3853
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1104 a         1313 c         1079 g         1662 t
ORIGIN      
        1 ccatccggac tgtgcatatt tccagtcaat gtcagaaagg taagtcactt ctccggtgat
       61 cggacgctca tttactttaa cggtgatcgc tgtgcttgtc agatctgcag atgctcctgt
      121 ggtatctttt gctgtcacat tttccagtcc tacgctgtag ctgccaggct gtgcatctgc
      181 ttttgctttc atggtgatcg ttgcaaaatc acttgtagat gccgggattc cagcttcgtt
      241 tttcagtgta taaagaatct ttccgtctgc ttctgtgctt gtcagcgtac cttcgatgcc
      301 atctttcaat gcaaccgtcg gtgcttcaaa gacatctgca tcataggaaa cgcggaagct
      361 tacttcacct aacaggttgt ctgaggaaag gtttgcaagt tttgcgttca ttgtcaggtt
      421 cttgccctgt accacagcat cttcgccttc gagtaccagg cgtgcaccaa gcttttcggt
      481 acttaatttc acagatgctt ctgtgatctc catgttgccg gatgttgtat aaggtgttgc
      541 attttccatg tacacacgga cttttgttgt gtaaaccggt ttgaactgca tcactgccgg
      601 tgctccggat tctttcacgg tactctgtgc ggataccggt gcccagtctg caccgttcca
      661 gtactgtgct tccagcactt tcgggattgc acagtagcgg ctctctttga agttcagggt
      721 gatctggttg agcacctggg cttcttccca gtcaaattct acatattcat tctttctgga
      781 tgcactgttt tccggaagca gtaccgatgc acctctgttg taagcattgg tccagcttgt
      841 gctgtcattt ccatccagca tattctgcgg ataattctta tcagatcctg tgaaggatgc
      901 cgttgcactt gctttgcccg ctgccagttc cacatcggtt gccgcatcat cactttcggt
      961 gatcgctgcc tgtgcggtaa gcttgctgcc ctctattgtt ccttctgctt ttccgtcttt
     1021 tacttcagac caggttactt ttttgagcag gctgtaatct cctgcactgc ctgtatagtt
     1081 tacttttact acagacggaa gtgtcacact cataccggtc ggtaccgtta cattcactgc
     1141 atcaaccgat ttttcggttc cggtcacttc tgctgccttc ggtgcatctc cggttccgtt
     1201 ctctgttact ttcagtgcga cctgtaccgg cttgagattg tcagaagaaa tggtcagcag
     1261 agcatcgcct gcttcttttt ctgatttcag aatcaccagt acttttccgt tgtaagccat
     1321 tctttcagaa taactgctct tatctacgtt atcgtattta tagagttctg cactttcctg
     1381 ttttccgttg tccacgccga cgatggaagc ttttccgctg actgcaaatt tcaccagatt
     1441 atctgcatcc ggtaccatat ttccattttc atcgactacg gtacattcta cataagaaag
     1501 gctggttccg tctgctttga gaacggtctt atctgccact gccgaaatgg tgtacggtgt
     1561 ggaagatgtg cttacactgt ctgttgccac tactttgccg tttttgtcat aggcttttac
     1621 ttccagtgta cctgcctgat acggaacttt ccaggtcagg tgcagttttc cggaatttaa
     1681 ctcgccgtca tcaagaactg ctccgttgct ggtgtaaccg ccagggttgg tgctgtcacc
     1741 ccaggtctta tcgtcctgtg ttttttctga tgtttcataa tatttcttac cataagctgt
     1801 ttctttttca tcgaaactct tcttgccgag agactcgccg tttaagaaca gctctgcact
     1861 tggctggttg gtatttaccc agacttctac ttcttcgccg tcatgccact gatcccagtt
     1921 ggtcggaagc agatgtacca tcggcgtgct tgtccactgg ctctgatata aatagaagga
     1981 atcttttgca aaacctgcgg tgtcgatcgt accgaaggaa gatacaccta ccgggtaaac
     2041 actgtacggt gtcggctcgc cgaggtaatc aaatccggtc cagataaact gtccgataaa
     2101 gctcttgcgg tcacggtctt ttttcagacc atattcattg gacatcgtcc aggatgcaaa
     2161 gtcgttgtca tagttggaac cgcctcgttt gcccggagtc tgattgatac cggtgttcgt
     2221 aaactgcgga tccaggtaaa cacctcttga tgaagtctga gaagaagact cggactcgaa
     2281 gaagaaggtc tttgttgctt tttcaagcag tgtgccatca ccgatagcgt atctgctgat
     2341 cagtccgtct acggattttg ctgtattgta gttcagtccg tatccgtcca gtacctggtt
     2401 tacataaccc catacgttgg ttactgccgg aacactacgc tcctggtcac cgcccatcag
     2461 cacgtaacgt gtgccgtctg ctgcattgac attgcccatc agacgaactg cctcggtata
     2521 ttcattcatg cctcctggat ttacgccaag tgcatcgtat ttggaaagat tataccagga
     2581 tggttgtttt cccacgccgc ggacttcatt accgatcgac catgcgatca cagaaggttc
     2641 attcttatcg cggtttacca tttcctgtac gacccagtca ctccaggtca gttttgcacc
     2701 atcgtagttt acgcccggtg tcggaagctg tgtataaccg ttcggtttca gtcctgccca
     2761 gtcagacggt acttcctgga agaagaaatt accaaaatca tacgttgctt ttgcttttcc
     2821 ccatccgtca aaagcttcct ctaccacaag gatacctttt cttctgcaga cttcgattgc
     2881 ctgtttggat ggcgggcagt gagaagtacg gtatgcgttg acacccattc ccatcagttt
     2941 gtcaaattca cggtcatagg catcgctgta gcttgctgct cccaatgcac cggaatcgtg
     3001 atgcaggtca acaccctgaa cttttgtata ctgtccgttt acatataaac caccgcttgt
     3061 cggatcactg gtcgtctctt tgacttttac ccagcggaat ccgtattcgg tttcttccgt
     3121 atcgatcagc tgatatccat cagaagaacc gttgatctcc tgataaagat ctactttnnn
     3181 nnnnnnngtt ccacggatac cagagattta cattggaaac ctcaacagca tctctgtctt
     3241 tcaaagacag ggtaaatgca gtacttgggt tgatcgctgt tttctctgta ctcttctttg
     3301 ctacttcttt gccgtctgca tccagcacgg ttacttccat ataaacgttg gagtttgtag
     3361 aatcagaata accggttgct tctacattca gtgcacctgt cttggatgcc gtataatcgt
     3421 cctccagagt cggagttgtc agtgtgatgc cgttgcggtt gaaatgtgcc tgattgtcga
     3481 tcaccagatg taccgggcgg atgataccgc ttcctgtata ccagcggccg gacggtgaca
     3541 tattctgcac ctttacaacc agtacgtttt cttcgccgta ttttactttt ttcgtgatat
     3601 ccagggcaaa tccggtataa ccgctcggat agctgccgat cttctcaccg tttaagtaaa
     3661 caacgctgtt ctgatatacg ccttcaaagt caagagaaat tgtcttgttt ccggacatgg
     3721 atgccggcac ggtaaatctc ttacggtaat agccaagtcc gccttccaga taagcctgtg
     3781 catcggaaga actctttact ttttcacctt caatactgaa atcatgcgga acatctaccg
     3841 ttcnnnnnnn nnntcattga aactcggatc aacaatctcg tctgttgtgt aatctccggc
     3901 atctttgaca cctgctgctg cgaaaccgcc atcagcgact tttggtgttc tcgttgccag
     3961 atagaactgc cagtttgcat cgaaatcaac ggatccaaga gaaccttccg gaacctcctg
     4021 aaccagacca gatgcatcca gaacctctac gttcatcact gcatcgtatg tggttccttc
     4081 tacggtaact tttccattta ccgtgaatgt cttgcctgct gttttggtat ctacctttgc
     4141 aagctcatcg ctgttccatg tcacatcaaa cagtgcattt gttccgtctg tatacagaac
     4201 ggttgcctgt gtcggtgcgg taaaggtact tccaacggtc actttctttg tgatatctgc
     4261 aactttatct accgtctttg gtcctgcttc ggatttgcgg taaagtttta cgttgtccag
     4321 attgatgtac tcgttggtca aagctacgga aaagctgacc gtacattttc cgtcttcgcc
     4381 gacttcaaaa gtatcggttg tgtaagttgt ccatttgtcc catgcggtcg gacgaaccga
     4441 tacgctttta tttcctgcca tcagagaagc agtcagactt cctttgtcat ttccacctgc
     4501 agcctcaact gaagctttgt aagtaccagg aaccagtcct tcgattgtct gtgtaaaaga
     4561 aaaactgtct tcatctccgg tatggctgta tgcctcatac cagtttgtct tattattctg
     4621 tgcccattca tttttgaaaa cacgccagta cttcgtgtct ccgtttgtgc cttcggttcc
     4681 gttaaacgtc cagcctgtgg tctgagactc aaaatctccg tttggaacag tgacttctac
     4741 aaacgtatca tcatctgctg ccataacgac tggtccgatg tttccgactg ctgtggtaaa
     4801 caccatggca aacgccagta aaaaggacca gaattttctg agctgtctgt gttctttcat
     4861 tccctttccc catcctttct ttgtttatag atcccggtgc tgtacgcacc agttttcggg
     4921 ttttcatttc tcttacagac tatcagtttt ttctgatcct ttccataata cattctatag
     4981 attttgtttt cattctatag attttattct attttctatt gttttttagt aaactgttta
     5041 ttcacctaac aggaaaaggt tttgccccct tacgttaaca tcatcccatg ttaatttttc
     5101 acccggaaaa cgtaaccacc aaaaatcatt aaacatcact ccatgcgtac gtctgttcca
     5161 ttcatatggg ttattctc
//
LOCUS       DAWDWH010000146         5169 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6618_length_5169_cov_13.530700, whole genome shotgun sequence.
ACCESSION   DAWDWH010000146 DAWDWH010000000
VERSION     DAWDWH010000146.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6618_length_5169_cov_13.530700"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1541 a         1051 c         1412 g         1165 t
ORIGIN      
        1 gcactggaag aacagggcgt gaaaatttta acatgcggga cttgtttaaa tcactatggt
       61 ctgacggacc ggcttgcagt cggagaagtg acgaacatgt atgagatcgc ggaaaagatg
      121 acgcaggctt ccctgattgt aagtccgtaa tatgagaaaa aaaacaaagc agctgttgat
      181 cacgttttcc accacgacag cggcgatggc gatggaaaag agggcacagg aaatggggat
      241 tttcgggcgg ctgattccgc ttccatccga gatttcagca gggtgcggac tttcctggaa
      301 aacgaaaccg gaagaaaaag aaaaaatgct tgtgtttttg gaagaagaaa agctgaagtg
      361 ggaagcaatg tatgagttag agttgtatta gatatgagat aaattgatat tcgggatgga
      421 atttgatttc aaacctgatg cagccaccgg cggattgcat cggtgtacag taagaatgtc
      481 agagataatt tttgaatagg gaaaggacag tatgggatat tttaatcaat acgtaagggc
      541 agaaagtctg gaagaggcct atgggcttta ccagaaaaaa aataattttg ttctcggcgg
      601 tatgctctgg ctgaagatgc ggaagcggcc gtttggaaca gcgatcgatc tgtcagatct
      661 tggtctggat cagatcgaag aagatgaaaa agaatttcgc atcggagcgt atgtttcgct
      721 tcgggaactg gagacaaacg aggcgttaaa tacttacacg aaaggtgcgt ttgccgagtc
      781 ggtgaaacat atcgtcggtg tacagtttcg aaatgtggct acggtcggcg gcagtctctg
      841 gggacgttac ggattttcag atgtgatgac aatcttccgt gccatgggtg caaaggtgca
      901 gctgcatcat gcaggcatcc tggatctgga tgagtttgta aaaatgccaa gaaagacaag
      961 ggatattctt gtttctgtga tcgtaccgaa aaaggtaaaa gcagtcgtgt atttaagtca
     1021 gagaaaccag tccacagatt ttccggtgct gacctgtgcg gtggcaaaac gggaagaagg
     1081 atatgtcgca gcagtcggag ctacaccgca tcttgcggaa aacgtctggg ataaagaagg
     1141 tattttaaca gaaataaacg aggaaagcat cgaaaaattt gccgctaagg ttgcagagca
     1201 gatccgtttc ggcagtaata tgagagccgg agctgcatat cgccggatcg tctgtcaggt
     1261 gcttgtaaaa cgtgcactgc atacgattgt ggcagaaaac aatggagagg aggaaaagtg
     1321 ctgatggaag tgaccattac attaaatgga aaaaagatca cagattcggt ggatgccgat
     1381 atggtgctcc ttgatttttg cagaaaacat aaatgttttt ccgtcaaaag aggatgtgaa
     1441 accggaaact gcgggctttg cagcgtttta ctggatggaa aaccagtgct ttcctgcagc
     1501 gttccaatcg gacgtgcatc aggaagaaaa gtagatacct tagaaggcct ccaggaagaa
     1561 gcaaagaaat ttagcgattt ttttgcagct cagggtgccg accagtgcgg attctgcaat
     1621 ccgggttata ttgttaatat cgtggcactg ctccgggaaa atccagatcc gacagacgaa
     1681 gagatccttg aatatttatc aggcaatctc tgccgctgta ccggatatca gagccagctg
     1741 cgttcgctcc gcaactatct gaacagcaaa aaagaagcgg caccgcttga tctggaggca
     1801 gaagtttatg gtgcggacat cggaagggag tggaagcatg agtgaattta agcgagatcg
     1861 aaaagattat catatggtcg gaaaatgtgt tccgaaaaaa gattcaaacc agcttctgct
     1921 aggaaaccca gttttcatgg atgatattgt gccgcaggac tgcctggtgg taaaactgct
     1981 ccgcagtcca catgcccatg ctctggtaga agaggtcaaa acagtggcag ctgccaaagt
     2041 tccgggtatc gaagccatct atacctggaa agatgtaccg aaagaacgct tctgtattgc
     2101 cggacagact tatccggagc catctccgta tgaccgtctg atcctcgatc agagggttcg
     2161 ctgtgtgggt gatccggtgg cgatcgttgc cggtgaaaat gaaaaagcag tcgataaagc
     2221 actgcgtctg atcaaggtga aatatcaggt tttggagccg gtccttgatt tcagaaaagc
     2281 ccttgataac gaagttctgg tacatccgga ggaaaactgg aaatcctatg gaaataccgg
     2341 tgatgtgaaa cgcaaccttc tgcatcacga ggaagatgcc cacggtgatg tagagcagat
     2401 tttaagcgaa tgcgatgaag tgatcgagca cagctggcgg atcaaggcgg cacagcaggg
     2461 ctatatggaa accatccgtg cttactgcga gatcgaccgt tacggacgtc ttcactgcat
     2521 cagttccaca cagatcgtct ttcatgtaag acgtatcatt gctaacgcac ttggaatccc
     2581 aaaatcgatg gtgcgtgcgg aaaaaccgcg tatcggcgga ggattcggag caaaacagac
     2641 agcagtctgc gaagtttatc cggcatttgt gacctggatg acaaagaaac cgtgtaaact
     2701 gatctacagc cgaaaagaat gtcagacgat cgcttcacca aggcacgaaa tggaagtgac
     2761 agttcgctta ggtgcgatga aggacggaca tatccgggct atcgaccttt ataccttatc
     2821 caataccggt gcctacggag aacatggaac tacgaccgta ggactttccg gacataaagc
     2881 acttccgctt tataccggag gctgtgaggc aaccagattt tcctgcgacg tagtctatac
     2941 gaatgttcag gcagccggag cctaccgcgg ctacggtgcg acgcagggta tttttgcact
     3001 ggaatccgca gtcaatgagc tggcggagaa actgcatatg gacccagtgg agatccgtat
     3061 gaaaaatatt gtccgcgaag gcatgtttat gccggcttac ttcggtgaga cggcaaatgc
     3121 ctgtgcactg gaccgctgta tcgaacactg tgccgacaac ttccactgga aagaaaaata
     3181 tccggtgcgt gacatgggca atggaaaagt gcgtgcagca ggcatggcac tggcaatgca
     3241 gggttcctgt atttccaatg tcgatgtcgg ttcctgtacc ttaaaacttg gtgacggcgg
     3301 aacgtataac atgatgatcg gagcggccga catgggaacg ggatgcgata caatccttgc
     3361 acagatggca gcggaagtgc tcgactgtga tgcagatcag atttccgttt tcggggcaga
     3421 tacggatgcc tctccgtacg actccggatc atatgcatct tccaccactt atgtgacagg
     3481 aatggcaacg cagaatgcgg cactgcagct gcgggaaaat atcaaaaaga tcggtgccgg
     3541 catgcttgag tgtgaaccgg aagaagtcga ttttgacggt gagaaagttt acctgaccga
     3601 cggcgaaaag tccgtcagtc ttgcggatat tgccacaaaa tcgcaggtca acaatacgat
     3661 tcctgtggat gtgacagcca cgtattcttc cccggtatcc ccgcctcctt acatggtcgg
     3721 aatggtagag atcgagctgg ataaagagac gggaagcatc gagatccttg attatcagtc
     3781 tgtgatcgac tgcggtatcc cggtcaatcc aaaccttgca agagtacagg cggaaggcgg
     3841 tatcggccag ggaatcggca tggcactcta tgaaaatgtg acttacaacg cacagggaaa
     3901 aatcgcagag aacagcttta tgcagtacaa gattccgacc cgtctggata tgggcaggat
     3961 caatgtagaa tttgaaacca gctacgagcc aagcggtccg tttggtgtca aatccatcgg
     4021 tgagatcgtg atcaacaccc cggcaccggc actcgcccat gcgatttacc gtgcgaccgg
     4081 agtatggcac cgaactgtac cgttcactcc ggaaaagatt ctgatgagca tgctcgatcc
     4141 acagtggaaa gaaatcgacc gtcgcgtcaa agaatcatga tacacattat ttatatggcg
     4201 gctggaaaca gccgccgttt tggaaatgaa aacaaactgc tccagaactg gaagggaaaa
     4261 ccactgtatc ttcatacatt tgaaaaactg cttcggatca gagaaaaaga gccggaaaag
     4321 atttccttaa cggtagtcac gcagtatccg gaaatttgtc gggaagtaca aaatcagccc
     4381 ggcgtgaatg cagtattctg caaagagagc agacttggtg tttcctatac gataaaggca
     4441 gggatccgtg cagttgggga aatatatgac tctgtaaaga agaaaatttg cccggtgcag
     4501 gaagcacatg attttgtgag aaaagagcat tgctttgtaa agaatgagag ttcttttgaa
     4561 agaaagacag agcatgtaag aacgtgggat tatctgatgt tcacagttgc cgatcagccg
     4621 aacttaagcg agcagagcat cgaagcactg attggggcag caggccagcg attcagacag
     4681 ggatatactt cgcagccgcc gacgttctct ctgtgctgtg gggaaagagt cggcaatccc
     4741 tgtatgttcc gggcagatct gatcccggaa ctaatgcagc tggaaggcga taaaggtgga
     4801 agagctgttc tgaaacagca tgtatgctgt tatgtggaga ttaaaaatga agaagaattt
     4861 tctgatatcg acacagaaga gcagatgaag aaaaattcaa ctgtcagttg aatgacaaaa
     4921 aggaaaaact cccttataat agggtcagaa cgaaacgaaa gcacggacag ggaggttttc
     4981 tgatgagcga aaaaatgtta gttacacagg cactggatga aagagacttg ctggtgaaaa
     5041 agattaacag caagatcgca aaggcaagtt ttgtcgatac gatcaaacca aacgaagaga
     5101 aagtcttcgc cggaaacatg aaaaaagaag actatgcaaa agaagcagaa gctgcatttc
     5161 agcagatca
//
LOCUS       DAWDWH010000147         5154 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6636_length_5154_cov_13.915474, whole genome shotgun sequence.
ACCESSION   DAWDWH010000147 DAWDWH010000000
VERSION     DAWDWH010000147.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5154)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6636_length_5154_cov_13.915474"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1488 a         1256 c         1051 g         1359 t
ORIGIN      
        1 tagatttcct tgaacctcta aatcccagac caccggcact tgcccatgat aaagcatttc
       61 cttctgcatc tgtcagagtt acgattgtat tattgaaaga tgactgaata tgtgcctgtc
      121 cgcgttcaac gtttttctta acgcgctttt ttgtcacttt tttggtaact tttttagcca
      181 ttttaaacta acctactttc ttttcgcttt ctatagttaa cttgatctgc tttgcaattt
      241 cctgctcagg tcttatttct tcttgtttgc aacagttctc ttaggacctt ttcttgttct
      301 tgcgtttgtc ttagtcttct gaccacgaac cggaagacct tttctgtgac ggattcctct
      361 gtagcatccg atctcctgaa gtctcttgat gttcagagcg atctctcttc tcagatcacc
      421 ttctactgta taatgctcgt ccattacgtc acggattctg cttacttctt cgtcagtcag
      481 atctcttacg cgagtatccg gattaacctt tgcatctgca aggattttgt tagcgcttac
      541 tctaccgata ccgtagatgt aagtcagacc aatttcgata cgtttttctc ttggtaagtc
      601 tacaccagca atacgtgcca tgtaattatt cctccatttt ctttgagaat gttatgtaca
      661 gcccgccatc cgggtatgat aacgtctgaa atgatgtgta cattttcatt cttccttata
      721 ttgtccctaa ttgaggctta cggcaaaacc gtccgcccag ccgcacaaaa accgtacagc
      781 ctttccgtat ttgcgacggt actcacaaat ccggtattac aagcaatata tcatggtctg
      841 cgacagtcag ccgatatctt ctgacacacg ggactgtcgt ccctttctca cccatatata
      901 ttgttatcag cataaacaac gccgcctggc ggttcatact gcagccgcac ttaattcaaa
      961 taaacagttc tcagtttgtt ataaattaac cctgacgctg tttatgtttc gggttctcac
     1021 agatgactct gatgcttccc tttctcttga tgattttgca tttttcgcaa atcggtttta
     1081 cagatgatct aaccttcatg gttctgcctc ctttcaccaa tgttacccct aaaatcaggt
     1141 gcaaatggac ataaaaattg cgtaattccg caaagtccga atatcagtat agcacctgtc
     1201 tttttaaatt gcaagcctat ttttctttct atataatgca aaatatattt ttgtggaata
     1261 tgtacagaat atatgctatt atatgtgcat aattatcggt attgtaaaga taccaggctc
     1321 aaaagccata aaaccatgca atacttacac aggatgtccg taggcagcat ctggtagatg
     1381 acgttaagaa tcatatggac aataacgata atggctcttt gcttgtcacg gcgtttcaca
     1441 aaagattcct atttcttttt gcagtaagga gatctgtcag attttacagc tgcatgagta
     1501 cactgtacta atacaggttt gagatagact ccggcacata gaaactgaac agacatcttg
     1561 tgacatcaca aaaataaggg agaaaaatat gattttaagt aaaaaaacaa aacatggatt
     1621 attgtctatg gggcttgctt tcggcatact gacctcatcc gtcgtttcgc ctgcatctgt
     1681 ttctgttctt gccagcgact attctgccag aaatgtgctg tctgatgctt atatccagtc
     1741 acagttcccg gcaccaatat cagaagagaa cataacctct gcctctgtcc gaagcgaggc
     1801 tttgcagctt cagagcagca aaagagcatt tgatcttgat ctgtccggtc agtcatttga
     1861 tggttccggc ggaatcgatt actccaaaaa tgccaagtac atggatgtat tgaaaaatct
     1921 gaaaacgact cagaacgatc agacgatcat ccttcgtttt aagacttcat ccgatggtct
     1981 gctctttggt gccggtagcg atgcactgac caccaccggt aaaaatatga cggtctcgat
     2041 ccgtgacggc aaactgcgtc ttgtgatgcg taatacaaaa aaagcagatg gtgcacctgc
     2101 cggcggcctg aagggttctt tcaatgtcag ccttgccgat ggaaactggc ataccgttgc
     2161 tatcagtttc ctgccgtctc ttaattatgt caatgacaac gtacgcatcg ccgtagacgg
     2221 cacggacctg aactatgctt ccaacacctg gggcagttcc tggaaagcag gctttaacca
     2281 gggagccggg gcagactaca acctgcttca gatcggcggc ggttcttatg tcggtgtcaa
     2341 caacaacgac tacgcttctg ccaattttaa cggtaagatt gattttatca ccgtgatcga
     2401 caaagcctac agcatggatg acctgaaatc catctcctac gaacaggtca gctcggtcac
     2461 tgccaaactc aatgccatgc gtcagagcgg aaccggcaag acatggctct tcaccggcgg
     2521 tacagaggcg gttgccgatt ttaaaaactc ctctaccatc cgtaactgga ttgggctttt
     2581 tgaggatacc atgcgcagcg gcggcagcta tattgaaaga ggacgtttcg tattcaatac
     2641 agcccgtcgc ggttccgatg ttgcttctat tcttgcagat tacgacagac agatcgatgc
     2701 ttaccacacg gaggcagtag ccatctcagt tggtgccgaa gactacagca aaggccaggc
     2761 aggactggat accttcaaag aaaatctgaa aaaactggtg gataaaatca atgcttccag
     2821 aaaacttgcc gttgtgatca ccccttatcc atccaaagac cctgtgcaga atacaaacat
     2881 tgctctttac aaagctgcca ttgatgccgt tgtctctgaa gatgcaaaaa tcgtagattt
     2941 ctcttctatc gcagtttcca atatcaacga tgacggcact ctgacactgg aaggtcatca
     3001 ggcggctgca aaccttctga aaaacgcact tggcttcgga agcactacaa cttcctacaa
     3061 ttttcacctg aaaaacctcg caccaggctc ttaccgcaca gaaaagaagg aaaatgctga
     3121 tcagatcacc gcaaaagcaa acggcaccac tctggaagtt catgcaggcg gcgtttctga
     3181 ctccggtgca aaacttacct acacgctgac ggacagtaac ggacagattt tctccggcag
     3241 tgcttctgct tctgatttca cggtatccgg tctgaaggca ggagaaactt acactttgac
     3301 ggtcagcgac aacagccgct ccgatggtat aacggaaacc tataaaccgg tcagcatcaa
     3361 gattacggat ggctctaccg gtacacttat ttctgaaact tccgattctg agtacgattt
     3421 ctctgatctg ctgaaatccg aaaaagcttt aacttacctg tttatgggcg acagtatcac
     3481 acatggcgtt gtaacaaacg gctacgacaa cgtaccgcag ctttttgcca aatatctgga
     3541 tgaactcgga agaaaagatg acgttgtgat caataccggc gtttccaacg ccaccatcgc
     3601 aacgactctg gatcagattg aaacgcggct gaaacgctac cagccggatg tcgctgtcat
     3661 tatgctcggc acaaacgact gtgcaaaaaa tggtgaaaac aacgtcgcag ctaccggctc
     3721 caccagcaac ggtgcgatca gcgttaccga attcaagaat cgttataaaa ctctgattcg
     3781 tgaaatccac aaaaacaatg ccaatacccg cattgttctg cgtgttccat gtgcaatgat
     3841 caattatggt cagcgtcagg agactttcga ggcgttcttt gcttcgatcc cggaagtagc
     3901 aaaagaaatg aaaacagaaa taccgggact ggagatcgct gttgtcaatc atcttgaaaa
     3961 ctggaacaat tatcacgata cggttcgcaa tgacaacctg agcaatccaa actacgcaga
     4021 tgcagatagc ctcaactgtg gctggttctc accggacggc ctgcatccaa acggacgcgg
     4081 caacatcgcc atgttccagc agatcatcaa agagctgaac ctgtatcagg caaattccga
     4141 actggcaaat ttctcttatg cactgagtga ctggacagat gcctccaata tcaaagtcgt
     4201 tgcagagcag aaagatgatc aggcttccct tgccatgaat cagtttgctt cttatacaaa
     4261 tgggctgcgt gatgtaactc tgacactgac cgcccagaac ggaacatcca tttccaaaac
     4321 aacagcttat gatgcaaacg gcagcatttc cctggataca ttggataaaa caaaagatta
     4381 cacccttcgt gtcaccggaa ctgataaaac aaacagcaaa caagtaacgt tcgcttctga
     4441 acttacaaaa gagatcacac cggacccgat tccagatccg gaaccaaaac cagatccgga
     4501 accaaaacca gatccgactc cggatccaac accaactcct actccggata agccgacaga
     4561 caatcaaccg tccaaccctg gcacttccga tacaacaaag gttttgaaaa agggagacat
     4621 cgttgctgtt ggcaagtact cttatcagat cacgaatacc gcaaaaaaaa ccgctgcctt
     4681 tgcaggtctt gccgatgaga atgtgaaaaa agtatcgatc gccgcttccg tgaagatcaa
     4741 caatactgca taccgcatca cttccgtcaa ggcaaatgcc ctgaaaggca aaaagaaaat
     4801 caccagcgta acgattggca aaaatgttgc cacaatcggt gcaaaagcat tctccggatg
     4861 caaaaatctg aaaaagatca ctgtgaaaac aaccacttta aagaaagtcg gaaaacaggc
     4921 atttaaggga atccataaga aagcggcagt aaaagtgcca agaaataaaa agacagctta
     4981 caagaaaaaa ttccgtggga tcttttcaag tgccaaataa aaagtgaccg agctctttag
     5041 agcgagggat gcttcttgtc cgaagggcaa gaagatgggc attaagaaaa cttatacgta
     5101 aaatatgacg ccatgatgat tcgaaaacaa tctcctgaca cgctgcgttt cctg
//
LOCUS       DAWDWH010000148         5115 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6685_length_5115_cov_6.683202, whole genome shotgun sequence.
ACCESSION   DAWDWH010000148 DAWDWH010000000
VERSION     DAWDWH010000148.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5115)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6685_length_5115_cov_6.683202"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    630..743
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1249..1258
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1838..1870
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3173..3182
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1086 a         1284 c         1060 g         1518 t
ORIGIN      
        1 ctgtctttta cgcccatttc caccagatct tcggcggaaa gatgtacttc cggcgtaaac
       61 atggttccgt cccacagaac ggcacagttg gaatagcttg taccgcctgc acggttgtag
      121 atcttgcctt tctggatctt cactgcatca aaacaaggtc tgccgtccga ataggaaaaa
      181 gcactggcat tgatgccgat gatcgatttg gtgcgtttta ccgcatgcga agtcgtctct
      241 cttgttccgc catacgtccc ataggaaaat gccgaaaaca tctggtttgg attgttgatc
      301 ttaatatccg cagtccagta gcaggtgctg tacgctctgt gtttgcgaag gatgactttg
      361 agcgtggaag tgcggtactc gatcttggtt gctgtcaggc ggcttcccgt gttcatacgc
      421 tcgcctttgt acaccgagcc ggtcgtgctg gtcgggtaat accagccgcc tttctttttc
      481 caggaagtac tccgctgttt accttttttc gtaaaatagt aggtggtatt cttgatcttc
      541 tgccacttgg tcagacgcac accgttttta tccagatagt acttattacc tttgtacttt
      601 aaccagccgg ttttctttac gccttctttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnaccagcc gcccttgaat ttcttgatcc actttccgtt
      781 ttcaccgaca aaatatttgc cgatccactt gtttttttcc agggctccgt tgtcgccgac
      841 gtgataattt ccaacccatt tgttgcggat caatctgccc agctggtcaa aataatagta
      901 atttttgccg atcagctgcc atttgccgcg tacattctgc agagtagatg gatcacagta
      961 gtacgtctgc cctttttcct gtatccagcc gacctttttg ccgtgtaccg tgcctgctgc
     1021 ctgtgccgga agacttgccg gaaatgccat gcacgccgca agtgctgcca caagcagtct
     1081 ttttttcatt tttccccttt gtttattccc catatggttc ctcctgatgc ttatattacc
     1141 tgtatttatt tatgctttct cctgttttgc gagtctgaaa tccatgacgt tctgacccac
     1201 atattatttt attctaacag aatctgccac tttctgcaag tctatgctnn nnnnnnnnga
     1261 ctgaccgccg tgactgcctc ccggctgtta tatccaaaga tcgaactgcc gctcaaatga
     1321 aactgcaaaa acagtgccag cagcagcatt gccgccagaa ggcgtgtaaa aaaagaactc
     1381 ccgttctgtg gaagttcttc tgttttttct gaaaaatcat tcactttttc tgccgttttc
     1441 cggtaaaccg gcatacggcc gctctgccgc cgcatctttt cgatctgccg tctctgctct
     1501 tcacttaagc ctgtatgcat aaaaatcccg cctttctgct atacgctatg caaaaaggcg
     1561 gggttttatt cggttatatc aaaatcatac ccttagcggt ttgcaagctc tctggtgata
     1621 tcatcccaag tcacaccgcg ttcagacatc agtaccatca catggtacag aaaatcggaa
     1681 atctcgtact tgatctcctc cggattcggg tttttcgctg caatgatgat ctccgtcgct
     1741 tcttctccga cttttttgag gatcttgtca atgccctgtt caagcaggta gttggtgtaa
     1801 gatccttctt tcggatgctg tttgcgatcc tcgatcannn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn gatggtcgaa aacgatcgcc ggattggtct catggtaatc tttctttaag
     1921 atctcacggt agaaacagct gcggttcccg gtatggcatg cggcaccgat ctgctgcacc
     1981 tttgccagga tggtatcgtt gtcgcagtcg atggaaagct gtttgacata ctggacatgt
     2041 ccggaggtcg cacctttctg ccacagttcg ttgcggctcc tgctccagta ggtcatcttt
     2101 ccggactcca cagtcttttc aaaagcttca cggttcatat aagcgaccat cagcacttcc
     2161 agatttttgt agtcctgcac aacaaccggg atcatgccgt cggagttcag tttcagctca
     2221 ttccagctca tcttgctctc gaacgtattg atctcaacgc catgcttttt cgcttccagc
     2281 ttggctgcca tcagatcttc attcagatca gagaatgtat ctccgcagac accgcagata
     2341 ttgtcgagca tcagaagctt cagcaggccg tgacggtctg cacgtgtgga aaccggaagt
     2401 accggcactt ctacaaggcg ggcagttgta cgcacatcca cttcatcgca gagcaggatc
     2461 acacgggagg tatattcatt taccctcgcc ttgtttgcct gcagctgtgc aaaactgtct
     2521 gtacagatcg cgattttttc ttttccgaaa cgtttggata cttcttctgt cagttcaata
     2581 ttggctgctt tggcataatt taagaaaacc tgctggcatc ctgcgtagag aagctttttc
     2641 acatcttcca gatttttaat atggccgcca ccactcatcg ggatatcgat cacccggctg
     2701 atctggcgga tcagattgag agactgctcg tgatctgcat cgtcatccga cagatcaaag
     2761 atcagaagct catctgcccc gttgttgctg taatgctggg caatactcat cacatcgcca
     2821 tcttcaagca gttcttttcc gtcaaagccg gtcactgctt tcccgttttt cagatacata
     2881 tgtaccacaa ttttcttctg cattataaac ttcctttcgt cgaaagcaca tctgtaatgc
     2941 ggctatccat tccaaccgcc atgtccagtg ctttcgcaaa tgctttgaac attgcttcca
     3001 tcatatgatg attatttccg ggtgtcaaga cttttatatg cagattcatt gctgccgcat
     3061 aggaaacagc atagaaaaat tcacgtacca tttcggtgtc catatccccg catttcggta
     3121 cggtaaatac atcatcaaag gataaatacg gtcttccgca cagatcgacg gcnnnnnnnn
     3181 nncgcacaca cgaccaggca ctcatccatc ggaagaatga aatgtccgta gcgttttatg
     3241 ccttttttgt caccgactgc ctcgcggatc gcattgccga gcacgatccc ggtatcttcg
     3301 atcgtgtggt gggagtccac atagagatct ccttttacgg aaagtttcat atcaaacagc
     3361 ccatgtctgg taaatccgtc cagcatatga tcaaaaaatc cgatgccggt actgatcttg
     3421 gattttccgc ttccgtcaag ggaaagcgtc attgcgatat ccgtttcttt tgttgtccgg
     3481 gtgatggtgg ctgtcctact cattggcatc ttcctcttca aaacggacac ggatcgaatt
     3541 tgcatgtgcc gtcagttttt ctgcctcggc aaactggatg atatctttgt ggatatcttt
     3601 gagtgcctct cttgaataat agatgatgct ggaacgtttg ataaagtcat ctacgccaag
     3661 cggggagaag aattttgcag ttccgttggt cggcagtacg tggttcggtc cggcaaaata
     3721 atcgcccagc ggttcgctgc tgtattctcc gataaagatg gctccggcat tgcggatctt
     3781 catcattact tcaaacgggt tggccgtcac gatctccaga tgctcagatg cgatctcgtt
     3841 tgcagtctca atcgcctcgt ccatcgtctc tgcaaccagt gcgtagccat aattgtccag
     3901 tgatttgcgg atgatctcgc ttcttgagag agtttttaaa tagccttcga tctcttcgga
     3961 aactttatct gcaaagtcac ggcttgtcgt caccaggatt gcagaagcca tctcgtcgtg
     4021 ttctgcctga gagagcagat cggctgccac ataatgcgga ttggctgtct catctgccag
     4081 taccaggatc tcgctcggtc cggcgatgga atcaatgctg acatgtccgt aaacagcttt
     4141 tttggcaagt gccacataga tgtttccagg acctacgatc ttgtctactt ttggtatact
     4201 ctcggttccg aatgccagag ctgcaatcgc ctgggcacca cccactttgt agatttcatc
     4261 tgctcctgcc tccatagcgg caacaagtgt ggacggatac acttttccgt cctttccgca
     4321 aggggtcgtc atgatgatct tctccacacc tgccactttt gccggtacaa cattcatcag
     4381 aacagaagaa ggataaactg cttttccacc cggaacatag acaccgactt ttgcaagtgg
     4441 catgactttc tgtccgagca tggttccgtc cggtttgctg tcaaaccagc tgtatttttt
     4501 ctgtttttcg tgatattcac gaatgttgac cagtgctttt ctgataacat ccagaagaga
     4561 cggatcaacc tgttcgtaag cttcgtcaat ctcttctttt gtcactttga tgtttccggc
     4621 attgatatcg gcaccgtcaa atcttttcgt atattcaaac actgccgcat ctttgttgtt
     4681 tcgtacattc tcaacgatct ctgccacagc atcctcgaat tttccatagc tgctcgggct
     4741 gcgtttcagc agattctcaa gcacgttttt tctggtttcc tctgtaagtg ctattgtacg
     4801 cattctttta tgcctcctgt ttgatcacag atttcagatc tgtcaaaatc tttgtaattc
     4861 tttcattttc catacgcatg ctcacctggt tgactaccac tctggcagat aacgggcata
     4921 cttcttcgag tacgaccaga ccgttttcgc gaagtgtgga accggtctcc acaatatcga
     4981 cgatcacatc ggaaagtccg acaatcggtg ccagctcgat cgaaccgttc agtttgacga
     5041 tttctactgt ctggtgtttt ttattataaa aatagtcttt tgcaatatgc ggatacttgg
     5101 ttgccacgcg gatct
//
LOCUS       DAWDWH010000149         5113 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6686_length_5113_cov_9.164690, whole genome shotgun sequence.
ACCESSION   DAWDWH010000149 DAWDWH010000000
VERSION     DAWDWH010000149.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6686_length_5113_cov_9.164690"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1266..1415
                     /estimated_length=150
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1957..1966
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3737..3746
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4504..4513
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1446 a          906 c         1321 g         1260 t
ORIGIN      
        1 gatggcagac gatataaatg gcgtcggatt tcaaatcgcg gatgaaatag cctcaaaagt
       61 cgggatccat acggattcag atttccgggt ccgcagcggt attctgcatg tgctgaccag
      121 gatctctctg gaaggtcatg tgtatctgcc gaagatggag cttctggcaa gaacggtgga
      181 gcttctgggt gtaagtcagg agctggtaga gaaacatatc atggatctgg cagtagaacg
      241 caggctgata ctcaaagagc aggagaagca gatttgcgta tatgggatca atgcctatta
      301 cacggaattg aatacagcaa aaatgctcca tgatctgaac atatcctgca agatcgatga
      361 gaagaagatc agcaggcgaa tggataagtt gggaaaacat tccgggtatg agctcgatga
      421 gcttcagaaa gaggcagtgg ttacagctgc cggtcacggc cttttggttc tgaccggtgg
      481 tccgggaaca ggaaaaacaa caacgatcaa tgcaatgatc gattattttg actcagaagg
      541 tctggaagtg cgactggcgg caccaaccgg acgggcagcc aaacgaatgc aggaggcgac
      601 cggctgtgaa gcacagacca tacaccgcat gctggagatt tccggggcag cagatgagga
      661 agaaggacca aaacagtttg aacgaaacga agagaatccg ctggaggcag atgtgatcat
      721 tgtggatgag atgtccatgg tggatattta tctgatgcat gctctgttga aagctgtcac
      781 cgttggaacg agactgatcc tggttggtga cagggatcag ctgccttccg tgggaccggg
      841 aagtgtcctg aaggatatca tagaatcaga atgttttccg gtagtgtgtc tgacacacat
      901 ttttcgccag gagggcggaa gtgatatcgt tttaaatgcc cataagatca ataagggtga
      961 acatatcgtg atggacaata agagccggga tttctttttt ctgaaacggg aagatgcaaa
     1021 tgtgatcatc agtgttgcac tgacactgat ccagaaaaaa ttaccttctt atgtagatgc
     1081 agatccgtat gatatccagg ttctgacacc gatgcggaaa gggcttcttg gtgtggaacg
     1141 tctcaatacg atctttcagc agtatctcaa tccaccgtca ccggaaaaga atgagaaaga
     1201 taccggcaga tgcaaattcc gggaaggtga caaagtcatg cagacccgca acaactatca
     1261 gatggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctgcg gtgtttttaa cggtgacatg
     1441 ggcattatcc gtgagatcaa tacatatgca gaaatggtga cagtggaatt tgatgagtgc
     1501 cgttttgcag aatattcctt taaagagctc gaagagctgg agcttgccta tgcactgacg
     1561 gtgcataagg cacagggaag tgaatatccg gccgtgatcc tcccgctgct gaacggaccg
     1621 aggatgctga tgaaccgcaa tctgctctat acagcggtca caagagcacg ttcctgcgtt
     1681 acgatcgtcg gaagtccgta tgcggtcaat caaatgatcg ataataaata tgaacagata
     1741 cgctacacat ccctgaggca gcggatctgt gaatttgtat tatgattcag gcactggagg
     1801 agatgatcac gctcctctat ccaagacgct gtccgctttg tcaggatatt ttaacagata
     1861 aagcggcact tatttgtatg aaatgtcgaa caaaagcagt accgatcagg ggaccgaaat
     1921 gtaaaacgtg tgggaagccg gtggcatctt tggaaannnn nnnnnntgga aacagagtat
     1981 tgtccggact gtcaggaaaa aacgcatttt tttacatcgg gaagggctgt ttttctctat
     2041 gaaaaagaaa tcaggaaatc tgtatatcag tttaaatttc acaacaaaag agaatatgcc
     2101 ggattttatg tatcggaaat ggagcgtgtc tgtggagatg agatccgcac ctggaatccg
     2161 gatgtgatca ttccgattcc gatgtataag agaaaaaaga gacaaagggg atacaatcag
     2221 gcggaaatac tggcaaaatg tcttgggaaa aggcttcaga tcccggttga tacaaaaagc
     2281 ctcgtgcgtg tacggaaaac gataccgcag aaaagtcttg gaagaaaaga aagagaaata
     2341 aatctgaaaa atgcttttaa aacttcgaca aatgcgttag aattaaagaa agtactgctt
     2401 gtggatgata tctatacaac tggggttacg atggacgaag cggcgaaggt gctttggatg
     2461 gcgggagcag aagaagtaca ttttcttgtg ctttgtacag gaaaaccaga ttgctgagag
     2521 atacttttca gagacaggat tttatgttat actgaaaaaa agacagagga atcattaagg
     2581 ggtaactatg aacaatgtct aaaattgcag ttatggaaaa gcaccatggg gaggaactgc
     2641 aaaaagccag agaatattat cgcagtgatt atgtggcagt acagctgctg aaaaatgtgt
     2701 gttatcttac tgttgcatat atgatcggac ttctgctctg gggagtggcc aatatggata
     2761 tgctgatcga gaagcttaac cgtatgcaga tcaaggggat actggccgga gtgatcatta
     2821 gctatctgct gtgtatgacc gcaggtctgc tgcttaccta tgtgataact atgaaacggt
     2881 atttttacgg gcaggagctg ttccggcact atcaggtgct gctgcagaga ctggaagatg
     2941 aagatgatat agaaatactg gaagtgacag atctcaggag gaaaaagtga agggaattta
     3001 taatatcagg acattattcg aaaaatatta cagtgaatat ggaaattaca tcgatgccgc
     3061 tgccaaattt gttacagcgt tgattctgtt tatgcagatc aatggtcgga ttggcggaaa
     3121 aagcattttt ggaaatattt ttgtggaact gattctggca ctgatctgtg cgattctgcc
     3181 gccgaatgtt atgattgtac tggcatttgt cgcagcactg ctgcagtttg cgtccgtatc
     3241 aatcgcatcg ctggcagtcg gaggaggcgt tttgctggca gtactgcttt tatattttgc
     3301 atttgcatcg agacaggcat gggccatgct actgacaatc ctcggacttt tcttccatgt
     3361 cccgtgtatc gtaccgatta gtttcgggct gatgggaagt acgatgtccg cagcaggtat
     3421 ggctgcggga acggtatttt actatacgtt attgacgata ggaaagctgg gcggtgcatc
     3481 ggtggatctt gcgatggcaa aacagagcac tgcggaaaag atccttgcag aactgcagac
     3541 aatgatcgat acgcttctgg gtgagcagga gatgatcctg atgctggttg tgctgctggc
     3601 agtatttgct gttgtatttc tgacaagaag gatggcgatg aagcatgcat ggaaagttgc
     3661 gatcgcagcg ggtacggtca tttatctggt gctgatgatg gcaggttaca tgatgctgca
     3721 tctggagatc gggattnnnn nnnnnnttta tggatcctga tcggaacatt gatctcggcg
     3781 gcagtggcat ttctgctgga acagttgttt tttaatctgg attaccgcaa aatagaaaat
     3841 cttcagtttg aagataatga ttattattac tatgtcgagg cagtaccgaa acgataccga
     3901 aacgatgagg acagtcagga aatggaagca ggaagaatcg acaggaaagg acgtgaacat
     3961 catgatgggc atgaaacaat tcattcatag ttatctttcc agccttacgt ttacttattc
     4021 cgatgtgttg gagatcatca tactttcctt tgtagtgtac cacatcctga tctggatccg
     4081 gaacacccgg gcgtggactc tgatgaaggg catcctggtc atgatcgttt ttatctttat
     4141 tgccacactt ctgaacctgg attccattct gtttctggtc agcaaaggca tcaatgcgat
     4201 ccttgtggca acggccgtta tttttcagcc ggagctgagg agggctctgg aacagcttgg
     4261 cgaaaagaag ttcctttctt cgctgatacc gtttgatatg gaaaaagatg cagaggaacg
     4321 tttcagcgat aagaccgtca acgagctggt gaaaggctcc tttgagatgg gaaaagcaaa
     4381 gaccggtgct ctgatcgttc tggaaaaaaa caccaagctt actgagtata taagaaccgg
     4441 tatcgagctg gattctctgg ttaccagtca gcttttgctg aatatttttg agcataatac
     4501 accnnnnnnn nnntccgcaa cctgttatct gcctctttcg gataacatgg agctgagcaa
     4561 agagcttggt acccgccaca gagccggtgt cggtatcagc gaagtgacag attctctgac
     4621 gattattgtt tcagaagaga cgggaaatgt atccattgca cagaaaggcg ttcttaccag
     4681 agatgtgaca ccggaagaac tgaaagaaca gctggttatt cttcaggata aaaatgtaag
     4741 tacaaaaaga tttaaattct ggaaaggaaa aggcaagaat gaggaaaata tcaaccagaa
     4801 taaagagaat attgaccagt aatcttggcc tgaaaatagt gtcggtgctg gttgcctgca
     4861 tgatgtggat ttttgtagtc aatgtggatg atccggaaaa agtgcagtct ttttcggatg
     4921 taaaagtgga aatccgcaac agtgactgtt ttggcgtgga caagacattc agtgttcagg
     4981 aggataccga taaagtaaaa gtatgggtga aagcgagaga atctgtgctg aacaagcttc
     5041 agtcaaaaga ttttaaagtg gttgccgata tgcggaatgt gacgctcggc aatgcagtgc
     5101 catatacact gga
//
LOCUS       DAWDWH010000150         5097 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6712_length_5097_cov_7.830226, whole genome shotgun sequence.
ACCESSION   DAWDWH010000150 DAWDWH010000000
VERSION     DAWDWH010000150.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6712_length_5097_cov_7.830226"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    239..319
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3181..3190
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1282 a         1298 c          984 g         1442 t
ORIGIN      
        1 ctcttacact gcccttccac ccttaccggc ttatggccgg cggtatattt ctgttgcact
       61 gtccctggag tcgcctccgc cggacgttat ccggcatcct gccctgtgaa gcccggactt
      121 tcctcgtctg acacctttcg ttttgccagc cgcgatcatt tatcctactc gcaaacataa
      181 gaatatctta acatttctca cattgactgt caacttccat tttgctataa tagctcatnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnng cgattgtgat aaaggaagtg tatcaatatg aaaaaatcaa
      361 ccaaagcaac cgattccgcc aacaaagagt cggtatttca aaatttatca aaggaagaac
      421 ggatagctct gactgtcttt ttccttacgc ctcttgtggc actgttgttc ctggaatacg
      481 caaacccggc ggggcccgga ctgattttct attctatccg tccgtcttgc ctgcttcgtt
      541 tcggtgtaac cgcattttta ttttatatcg tgatgggatt tctgtttgcc ctgaccggaa
      601 gtatgcggtt ctccggatgt tttctgtgta ttttcagcat tatattttca ctgacgaatt
      661 atttcacaac aacgtttcgc ggaatcccga tcctggcttc ggatctgacg atcatgggaa
      721 ctgcgatgaa tgtggtcggc aattataaat attcgcttga tctgacccgc acgatcacac
      781 tgcttggtct gatcacctgg tgtattctgc tgtttcgtgt aaaacgtctc cgacttccaa
      841 aaggcaaaaa aaggatctct gccatcctag gaagtgccgc catctgtttt gcttccttct
      901 ggatcatgat ctatacgccg gttatgactg tgacaccgat gcatgtcact gtcaatacgt
      961 tccgtccgat caaatcctac cgcaaaaacg gatgcgtcct gacttttatg cgaagtatcc
     1021 agttgatgat aattcataaa ccggatggat attctgcaaa cgctgctgaa aagatagccg
     1081 ccccgtatcg cagtgaaacc tcttccggca atgcaaaaac accaaatgtc attgccatta
     1141 tggacgaagc ttttgccgat ctgcaggccg tcggtgattt caggaccagc gaagatgtaa
     1201 tgccacttta ccacagtctt acaaaaaaca ctgtaaaagg gttctcctat gtatccgtat
     1261 tcggagggca gacggcaaac acggaatttg agtttctgac aggactgtcc aaagcttttg
     1321 tgccggcatc tgcaacacct tatcagcttt acatcaaaag tcttctgccg ggtctgacca
     1381 cacatcttgg aaaccaggat tatcagggta tgctggcatt ccatccattc cgtgcaaacg
     1441 gttacaaccg cgaccatgtt tatccaaacc tcggattttc ggatttcatc agtttaaaag
     1501 atctggatgt ctccgcttcg gataagattc gcaactttgt atccgacgca gctgatttcc
     1561 aggtgatcat cgatcagtat gaacaggcaa aaaagaagag caatgccccg ttttatctgt
     1621 tcaatgtcac catgcagaat cacagcggct atgaccagga ttttgacaat ctggatatgc
     1681 cgatctccat cgaagaaaaa tgtgatgatc cggaactgaa acgttacctg aacctgatcc
     1741 atcattccga cactgccctg aaatctctga tcgagtattt cagcaagcag aaggatccga
     1801 ccgtaatcgt ctttttcggt gatcacgaac ccggactttc caatgaagtt tacagcaaga
     1861 tcctcggaaa aaatgtcgaa aaactcagtg cagaagaaaa tatgaatctc tataagactc
     1921 cgtttctgat ctgggcaaac tatgatatcg aagaacagga aaatgtaaac atcagtatga
     1981 attacctctc gactctgatg cttgagagca ccggcatgaa actctctccg ttcaatcagt
     2041 tccttttaga tatacataaa cagatcccgg tgctgacaac aaacggttat ttcggtgagg
     2101 atggcaacta ttattcctta aaagatgaat cctcccctta ttatgaatcc ctcagaaagt
     2161 atcagatcct ccagtacaac aatctgtttg acaagaaaaa aaggatcgag aacttttttg
     2221 actgatgtaa gagaaaaaga aaagccatgg aaaagacaat atttgtcact tccatggttt
     2281 ttgttatatc tgaaaacatc cgcccccgat aaactctctt aagatagaat tcatcggcac
     2341 gctctcgctt gggattccca caaaatagtc caaaaatttt aagaggcgtt tcgcttcctg
     2401 gtattccggt tcctctcttc cgatgccaaa caaaagcggc tctgcataca tcttcagaac
     2461 agccagttca tagatcagtg ctgaattgaa catcacatct gcctgctcct gataaggaaa
     2521 aatataggac tcttctcctc ttcgcaccga cggccacata ctgatcgttg cttttgccgt
     2581 cgttccccgc gtcctggcat cgcgtacaat ccttcgaagc agtcttccgt ctgtagtcgg
     2641 aacacggtta tgttcatcga cgttgagctg cgtcagggca ctgacataaa tcttgaattt
     2701 attttctttt ggcagtgcaa aggacaactg atcattcagg cagtgaatgc cttcgatcac
     2761 cagaatatcc tcttttccaa gttttgtctc tttttcactg tacatgcgtt tgccgctcac
     2821 aaaatcaaaa acaggcagat gcacgctctt tcctgcaagc agtgcgttca tgtcatcgtt
     2881 aaactgctga acatcgatgg cttccagaca ttcgtagttt ggtttcccat cttcatccac
     2941 cggtgtgtct tcccggttta caaaatagtt atccaccggg atcggatgcg gtctcagtcc
     3001 gtgtgcggaa agctggatcg aaagccgatg agaaaaggtc gtctttccgg aagaagacgg
     3061 accggcaatc ataatgatct ttttatctgg attttcggcg atctttccgg caatctccgc
     3121 aattttcttt tcctgaagtg cctcctggat caggatcagg tcatggatgc catggtgcac
     3181 nnnnnnnnnn gcacaatata atcattcagt aatcctacgg taggaatttc gagcatctct
     3241 ccccatttga gcgattcttt tcgaacacgg aacattttgt tctccggctc aaaggccgga
     3301 accgtcttcg gatctttccg ctgtggcatc tgaagtacaa atccgtcctc atacaaatgc
     3361 agggaaaacg ctcccagata agaagtgtcc ggaatcatgt agccataata ataatcttcg
     3421 aaaccgccca tatcatagat attgactttg gaagcccggc ggtaatgaaa caatttttct
     3481 ttgtctttca tcccgtactg cgagaacaat gcgatcgcat cgtccgtacc gatcgttttt
     3541 ttgtcgatcg gcactttctg ggcaaccagt tccctcattc tggcttccac tttaccaaga
     3601 aaatcctgat ccagtgtcac atcaccgtcg accgtacagt aatatccgct gctgacggaa
     3661 aaatggatcc caactctgcg gatgtgttca tgccctgcct gatggtagag tgccttcagc
     3721 aaaagcagcg tcatactccg cctgtaggcc gccatccctt cctgctgcct tgttgtgata
     3781 aaagaaatgc ggcagtcctc cgttaattgc aggtacagtt cccgcagccg cccgttgacc
     3841 tttgcaagaa caatatcatc ctcctactga tattcctttg caagatcaag cagccgtata
     3901 cccgccggat agcttttcct ttctatgttt ccattttcaa aagaaacctg aatatattct
     3961 ttctccatca tcaaaccacc tcctgatcat ttttacagat cacattgtca tcgataccgg
     4021 aagattccgt tctgtgactt gaaattctcc gaaaacttat ctacaccatc aaaaacggat
     4081 gtcggatcgg ctctgtacag atttccattt caggaaaacg ctcctttaat tcttctgcca
     4141 tctgtccgat aaaaatatgt tcaatgccat aatgaccggc atcgatcaca ttcagtccct
     4201 ggtctacagc atcaatccca tcgtgatggc caaaatctcc ggtgatataa acttctgccc
     4261 catttcggag ggcatctgcc gtcatactct tcccggagcc cggaagaacc gccgcacggt
     4321 tcagaactgt ttccggattt ccgaaaatct tcacatccgg tatctgaaaa acatctttta
     4381 catactctgc acactgaagc agtgtcatct cttttggcag ttcgcctgcc ataccaaaac
     4441 cgccttcacg tccatccgga agcacacagg ttttctccat gatctgtgca tccttcaacg
     4501 caagcatcct ggctgctaac ggtgccatct ccacgatgtc ataattggta tgcattgcat
     4561 aataagcgat gtcatgcttt atcagttcca gcacacgccg tccgtaaaaa tcaccgtcat
     4621 ttattttttt cagtccatgc atcagcatcg gatgatgcgt cagcagcaga tctacttttg
     4681 catcgatggc atggcggatc acttctccgg tcgcatccag tgcgatataa atgctcttga
     4741 tctctttctc atagcttccc acctgcagtc ccggattatc ccaggattct gcgatggatg
     4801 gcggatattt tttctccagt aattcaatca gctcccggca gatcatgcct tctcacactc
     4861 ctcttctgtt ttcatccatt tctgtgcttt ccggttcagg gcaagcaatg cctctatttc
     4921 caaaatacgc tctttcaccc tctctttggg ctgctgcctt aaattctgaa gcacaccagt
     4981 cagcgtggtc tcttcttttt tcagaaaaga gccaagaacc ggattctttt cttttaataa
     5041 aaaagctccg tatttccatt ctatgtctgc aagttcttcc atatgtccgt gctgcac
//
LOCUS       DAWDWH010000151         5084 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6728_length_5084_cov_8.744482, whole genome shotgun sequence.
ACCESSION   DAWDWH010000151 DAWDWH010000000
VERSION     DAWDWH010000151.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6728_length_5084_cov_8.744482"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1692..1701
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4389..4398
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1164 a         1328 c         1006 g         1566 t
ORIGIN      
        1 tcagctttta tctgatgata gcctttcttg ccttttttga tcaggagaac gccgtcttca
       61 tcaaaatctg cactggtaaa tacttttgca aaatccgtta cttttacatc attgacagaa
      121 acaccgccct gtgttaccag tcttcttgct tccgaacggt tctttgcaag tttggtatcc
      181 accagaagtg tcaggatatc tttgccttct tccagctctg ctgccgggta agaagtagtc
      241 gggatatctg cggtcttgcc gccctgtaca aacagtgctc ttgcagcagt ctgtgctttc
      301 tctgcttctt cttcgccgtg tacgatcttt gtaatctcgt atgccagcac ttcttttgct
      361 ttgttgatct ctgcaccttc cagtgcaccc agtcttctta cttcttccat cggaaggaag
      421 gtcagaaggc ctaagcattc ttctactttg acatcttcga tgtttctcca gtactggtag
      481 aattcgtacg gggaagtctt ctccggatcc agccatactg cacccttcat ggtcttgccc
      541 atcttgatgc cctcgctggt ggtcagcagc ttgaaggtca ggccgtaagc cggtttctgt
      601 tcttttcttc ggatcaggtc aacaccgccc agaatgttgg accactggtc gtttccgcca
      661 agctctatct ggcagccgta cagctggttc agtttcagga aatcgtagga ctgcatcagc
      721 atgtagttga actcaaagaa cgtcagtcct ctttccattc tctgtttgta gcactctgca
      781 gtcagcattt tgttgactga gaaatgtacc ccgatttcac gcaggaattc tacataattc
      841 agatccagca gccagtctgc attgtttgcg atgatcgcct tgtcattgtc aaaatcgatg
      901 aatctggaaa gctgttcatg gaaacgcttt gcattgtaat cgatcttttc ttttgtcatc
      961 atatttcgca tatcggattt gccggacggg tcaccgatca ttgtggttcc gccgccaagc
     1021 agggcgatcg gtctgtgacc tgctctctgc atgtgcatca ttgccatgat cgtcaggaaa
     1081 tgtcctgcgg tcaagctgtc tgcggttgca tcaaatccaa tgtaaaatgt taccttttct
     1141 ttgcccagaa gctctctgat ctctgcttcg tgcgttgtct gctcaataaa tcctctttct
     1201 ttcaggacgt caaatacgtt ccatcgtttg attcctcctt agactttttg ttttccaagg
     1261 gcgtaaaagc gtagaaagcc ctcgcccttc ttatacgaaa aggacgagag ctgaaatctc
     1321 gtgttaccac ctttattcac agccggctca caccagactg cctcagtaag tactgccatc
     1381 ggaatccttt catgtaaaat ccttttgctg ttcttcgcaa ttttttacct atccacagca
     1441 atactttaac gttgttaacg gatgtcactc cgctgcagcc tacttacact tctgtgttcg
     1501 gtgcagagct ccggaatgta ttcgcaggaa ccgcatctgt gcctctcacc ggccggcaac
     1561 tctctgttgt gcagttgttc tgttacttct ttccttcaca gcctttgcct catttttatg
     1621 tctcatgcgg atgtcagaaa gattctgttt tttttccgta cttccacaat tttgacatta
     1681 tcctcattat annnnnnnnn nctaccgctt tctcattata ctcatcattt aacgctgaca
     1741 cataattctg gatcggcagc tgtttctggt atacggtaaa ttcatatgcc cagtaatcat
     1801 cctccgaatc aaactggtcg atcacgtctt gaatatcttc gctgttgtcc acgctttcga
     1861 gctgtagctt catatcttct atatatgcct gcacctgctc atccgtcaca gaatagccgt
     1921 ttttgactgc cgctgcgtaa agtgcattgt actgcttgat ctctttatcc gcatctttcg
     1981 ccgcctgccc gtcatcttca ccttttaatt tataaaaaga tttgacctgc tcgtattctt
     2041 tcatcgtgat tgagacatct tttccgactg catagagcgg ttcgtcgttt gcactctgcg
     2101 tgtttatttt ttcttcatct gccgactgtt ttaaaatttc accccaaagt gccgaaaccg
     2161 attttccctg atttcctgca taaactgccg aaacagaaag aactgccacc agagctgcgg
     2221 ctgcgatacc cgttgcaagt tttctttttt tcatatagat ccaacctttc ctactatttt
     2281 gttttgagtg caaatgatat agaaccagaa tatgtaggta taactgctcc tgtacagcat
     2341 acccaaaacg gataattcac tttcgctttt gtagctttaa aagttgtcaa gtttcaaacc
     2401 gatacgatct ttctatgttt ttctgcaacc atttcatcag caacttgtct gatcattttc
     2461 cccaacaaaa aatcgctata acatcagcca tcactgatat tataacgatt ttcgttttgt
     2521 tttttctaca ttctgtcaca cttttcaatt ttcagatccg gataaacggt atagccccat
     2581 tcttttgcat tgcggttcag gctgtactgt ccaaatgcat cttctgtaat ctcaaatgca
     2641 aaggcatgtt ccagccaggc atgccgccac tgtctgccgc cgcccatcgg ttccacggcc
     2701 gccagctctg ccacatattc tccttttgta aggcttgtgg tatcaaagtg aatccccacg
     2761 cctgctgaag tattttttgc aaacgccccg attccttcgg aaacggatgt gcccactgcc
     2821 tggccgtccg tactgtggat ggtacagcga agatgcagat ctgcaattgc ttcctgcact
     2881 cgaaacgcca cggaaaatcc gattccttct tttgccgtta cctgattgct gtttttgata
     2941 tcaaggcttg taaactgcat ttttccggtg gtcctgcggt catttgcatc tgcttcataa
     3001 gtatagtgta atttcatctg cacaacatca cccagatatt ttgcgatcgc ctcttccggt
     3061 tcgccatcaa aaacaagatg tcctttctcc aggacaatgc agcggttgca gagtgtgcgg
     3121 atggtttcca tattgtggga tacatagaga atggttttct gctcatcgtt tgagagctgt
     3181 gtcattttat ccagacattt cttctgaaaa tgcatatctc caactgctaa aacttcgtcc
     3241 atcaaaagca gttcggaatt taaatgtgcc gctacagcaa atgccagttt cacgtacatc
     3301 ccgctggaat aacgtttgac cggagtatcg ataaattctt cacattcgga aaatgcaatg
     3361 atctgatcga tacaggcatc gatctcctgt cgtttcattc cgaggatggc tccgttcaga
     3421 taaatattct cccgcccggt cagttctccg tgaaacccgg ttcctacttc gagcatactg
     3481 gaaatccttc cctttacgcc gatggctcct tcggtgggtg ccgtcacacg tgccagcagt
     3541 ttaaacagtg ttgattttcc ggcaccattt gcaccgatca ctccgatccg ttctcctctg
     3601 tgcacatcaa aggaaacatc ttccagtgca taaaaacgct cttttgatgt atattctctg
     3661 cgaccgatcc gctgatttgg atcaggtttc ccacgttttc ttgcccacca actctggaga
     3721 tctctctgaa gtgtcccccg tccgatcacg ccaagccgat attcttttga gagatggcgg
     3781 atacggatca caacttcttt ttcttccatt tcttttccct ttctgagcac aggcataaat
     3841 gctactcgct gtacgcctgg caattttcca tgctatgatg ctagacggta tccataaaat
     3901 ttcgctgtgt tttattgaac atcaccgttc cgacaaacag aatcagcagg atgatcccca
     3961 gcccgatcat caggttcggt atggaaacaa tgccgcttcc aagccagcca taacggaaac
     4021 attccacaat gtacgcaacc gggttcagac tgagcagtat tctccatttt cccggaattt
     4081 gtgaaagcgg ataaacaatc ggactggcat acatccacag ctgaatccca aatccgacca
     4141 gtacctgaag atcgcggtat tttgccgtca gggatgccag gatcattccg gtgccgattg
     4201 cgagcaatgc cagcatcaca agcaagaccg gtgtcatcag acacagccag gtgattctta
     4261 cttgaccgct tccaaggctg caaaacaaca ggatcagaaa cagcaggaac tgtgcaccga
     4321 atgtgatcag tttagagaca gcggatgatg ccggaacaac aatccttgga aaatatactt
     4381 ttccaaacnn nnnnnnnncc tgccatataa aacagaaatg caggggttcc atcggtggat
     4441 attccggcaa tcctgccaaa gatcagactg aacatccccg ttgtcaaaag cgggcttaac
     4501 aatatccacg ccgggcccaa aatggtctgt ttgtacatcg ttttgaaatc tctccgcaca
     4561 aacagaaaca gcagatcccg gtattcccaa agttctttta ttggaaaatg aaatgccgaa
     4621 gcataagggc gtatctccat cgtccagtcg tctgatccaa gatcgacttt ttctgcctgt
     4681 ctcataattt atactccctc atccacaatg agatccgttc cgtctgccgc cgtggtcgca
     4741 agggtatcgc agcgttcgtt ttccgggtgt ccgtcatgtc cttttaccca gataaaagtc
     4801 acctgatgcg gctcctttgc tttcagcagg cgtttccaga gatctgtatt tttaacaggt
     4861 tcatttttgc ctcgtttcca gccttttttc agccagccgt caatccagtg ttgattaaag
     4921 gcatcggtca gatatttgga gtccgaataa agctgcactt cgcatggtct ggtcagcttt
     4981 tccaaaccgg tgattgctgc catcagttcc atgcggttgt tggtcgtccg tacatatccc
     5041 tggctgtatt cccgctcatg cagcacacct ttgctgtcga tata
//
LOCUS       DAWDWH010000152         5061 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6757_length_5061_cov_11.733919, whole genome shotgun sequence.
ACCESSION   DAWDWH010000152 DAWDWH010000000
VERSION     DAWDWH010000152.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6757_length_5061_cov_11.733919"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3385..3394
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1298 a         1251 c          975 g         1527 t
ORIGIN      
        1 ggaatagccg ttccgcagta gcggaactgg cattccgttt gaacataatt tgcagtcaaa
       61 tgcagtaaaa cacagtgctt tcatattttt gtaatcgcag tgtgttccaa aattgtgcca
      121 cagtccgaga gacagctccg gaagcagcag tccgctgttg ccgcatcggt tatactgcat
      181 ggtttcataa cgtttttcgt ctgcctgata catagtaatt tcctccttat gatctcatgg
      241 caatgtctgc cagttctttg caatctttct ttttactctt tacaaacgat aacacttcaa
      301 gtacacttga agtcaagaac tttttctata tttttgcata aaggagatgc gtcatggata
      361 cctatacaat aaaagaagtt tctgaaatgt tccatctgcc ttcttctacc cttcgctatt
      421 acgaagatct tggcatcctt acaaacgtcg gacgcagcaa atccggccag cgtgtctata
      481 tacagggaca tatcaaccgt ctgaagacca tctgctgctt taagaaaacc ggcatgtcga
      541 ttgccaaact gcagcagttt tttgcctgcg aagaagacga atctgctcat atcgatgaga
      601 ttttaagtct gctgaatgac cagcaggaac acattctgaa tgagatcagc caatatcagg
      661 cttctcttac acatctgcag cgaaaacttc attattatac agacatcaag acaagtctgg
      721 aaaatggaac tccgctgccg aaatgggcag attaccgaga gcatgcagaa caggcaaaat
      781 aacggcttga ccttaacatt attaaattta tttatccgga ggtattttac tatgaaattt
      841 atttatcctg ctattttcag caaaacaacc gaaggcacct tcgaggcccg tttcccggac
      901 ctggaatgct gcacggcaac cggcgagaca cttgacgact gcattgaaaa tgcaaatcag
      961 gctgcctatg actggatcta tgtcgaactg accgagtttg attctgagct tccgccgatc
     1021 accgacccgg atgatctggt gctctctgac aacgaaacat tgcgaaatat ttccgtaaat
     1081 attcgcctca ccgatggctg ggacgaataa atactctgac agttccatac tatttataaa
     1141 acagttgaca taacttcttg caaaatcttt ccctatccat taagataaaa aggtatgtta
     1201 attcaaacct gaggaaagga aaggtagaaa acacatggaa aagttcttca aactgaagga
     1261 aaatggtaca aatgtcagta ccgaaatcat ggccggaatc acaacattca tgacgatggc
     1321 ctacattctc gcagtaaatc ctaacatcct gtctgcatcc ggcatggatc gcggtgcggt
     1381 atttactgca acggcattat cagctttcat cgccacctgc ctgatggcac tgctgtcaaa
     1441 ctatccgttt gttctggctc ctggtatggg actgaatgct tactttgcct acaccgttgt
     1501 ccttggtatg ggatatacct ggcaggcagc actcgcagcc gtatttgtag agggtattat
     1561 tttcatcgtt ctctctctta caaacgtacg tgaagcgatc ttcaatgcaa tcccgatgaa
     1621 tctgaaacac gccgtatcgg taggtatcgg tctgtttatc gcatttatcg gtctgcagaa
     1681 tgcaaaaatc gttgtaaaca atgactccac actgcttggt ctgttctctt tcaagggttc
     1741 tgttcaggcc ggaactttca attacgaagg catcaccgtc ctgcttgctc tgatcggtat
     1801 tctgatcact gcgatcttcc tgatcaagaa cgtaaaaggc ggtattctct ggggtatttt
     1861 gatcacctgg ggacttggaa tcatctgtca gttcaccgga ctttatgttc cgaatgcaga
     1921 cgctggtttt tacagcctgc tgcctgactt ctcaagcggt ttttccattc cggctctgac
     1981 accaaccctg atgaaaatgg acttttccat tgtattctcc cttgactttg ttgtcatcat
     2041 gtttgcattc ttgtttgtag atatgtttga cactcttgga accctgatcg gtgttgcttc
     2101 caaagcagat atgctggata aagatggaaa gctgccgaac atccgcggtg ccctgctcgc
     2161 tgatgccgtt ggtacttccg taggtgctat gttcggtact tctaccgtta ccacattcgt
     2221 agaaagtgca tccggtgtag cagaaggcgg acgtaccggt ctgacttccc tgacagcagc
     2281 gatcctgttc ggactgtccc tgttcctgtc cccgatcttc ctggcaatcc cgtctttcgc
     2341 aacggctcct gccctgatca tggttggatt cttaatgctg acctctatca cgaagattga
     2401 tttttctgac tacacagagg ctattccatg ctttatcgca atcattgcca tgccgttcat
     2461 gtacagtatt tcagaaggta tcgctatggg cgttatctcc tacgttgtac tgaatgttgc
     2521 gaccggaaag gcaaaagaaa agaagatcag tattctgatg tatgttctgg ctgtgctgtt
     2581 catactgaag tatgttattc tgtaatgtaa atgatgttgg gatcaaaagc cccaaactat
     2641 gatgcctgcg gattgttccg tgtgtcgcac tcccacaata tttctacgct ccgcttcggt
     2701 cggtgctaaa catgtgccac tggcacatgt ttagcacccc ggtattccca tatcgtagtc
     2761 agaatgtaaa attgactaaa ataaaagcca gaacatatcg tctgcagcaa ttcactgtca
     2821 gccttatgat tctggctttt tctttaaata aactgtttta gatccgtcag tgtcggcaca
     2881 catgcataca gcatatcttt gatttcttca tccggctgtg tcagatccgg aaccataacc
     2941 actttacatc cggccgcaca ggcacttttg atgccgtttg gtgaatcctc aaccgccata
     3001 cattcttccg gggcaagtcc gatctgactg catgcatatt catagacatc cggtgccggt
     3061 tttccgtgtt ccaccatcgg tgcacagaca atcttgtcaa aatactcata cagtccaaac
     3121 tgtttcagat aacgtgtcgt tctctccaga tcactggccg ttgcaactgc acggtgtata
     3181 ttcttatctt tcagataagt aagaatctca actgcccccg gttttatatc cactttgact
     3241 gtcttcagat attcttccat aatttttctt cggtaacttc ttactgcttc ataatccagg
     3301 ttcggatcat tcaccatttt tttcaaatac tcgggtgcat atggcctccc cagagacctc
     3361 agtgtcagaa cctgttccct cgtannnnnn nnnncgaaat gttccagagc ctttggccag
     3421 aacataatat aatatttctc cgtgtccagt aacgtgccgt ccatatcaaa aataactgct
     3481 tttatcattt gttctcttcc cccgtcagtt ttacgaccgt catctcatga tgcatcgctt
     3541 tcatcactgt atcaaacata tcttttgttt tcacacggat actggaagta ttcacacacg
     3601 gatgacatcc gatccattct tccttgagca gatcctcatc caccagaaga cgtactgcat
     3661 tgtctttgtc attcatcagt cccataatgc tgaccgctcc cggcgtaata tccagatatt
     3721 tttccatata ttctgcactc gcaaaagaaa gcctcgcact gccgatctgt tttgagagtt
     3781 ctttcgtctt gaacaccttg tcccccggta tcaaaagcag ataaaaatcc gtcttctgcc
     3841 ggttgcacag aaacagattt ttgcacattt ccacgccaag tgcctcatcg acctctttgc
     3901 atgcttccat tgtaaaagct gcctcatgat ccatcctctg ataatctaca ccaagaccat
     3961 ccagaaaatc ataagtacgg atctccttgt ccattctgcc tgctgcatcg gcaggtcttc
     4021 cattctgtaa ttccatgttg ttgtctcctg ttattcctct gtatacctcc gcgatccgcc
     4081 ggaggataca gcaatcctgt gaaaaatcaa atccctccac tgatataagc taaaagtccc
     4141 ctgcatccac gcagaatatc atcgtaagtt gcatcaaaat cccctgtata ccacggatct
     4201 gccacgtcac gtgccgctga cagctgatgc gtcttctcat aatccgcaaa agaaagcatc
     4261 ttataaatct tatgctcctc atcaccgccc acaatccgtg tcatgtttct tatattagca
     4321 gaatccattc caataagaaa gtcaaaattt tcataatcct gccgcgtcat ctgcctcgca
     4381 tgatgtgcaa tcagcggaat ctgcacctgt cgcagcttgt ttctcgttcc ataatgcggc
     4441 ggattgccga tctcctcacg gctggtcgcc gcagaatcaa tctcaaagca gtcttcaagc
     4501 cctgcctgac ggaccaggta agtgaagaca ctctgggaca ttgtgctgcg gcagatgttg
     4561 ccgtggcaaa caaatagtac ttttatcatt gttttgtttc tccttgtttt gccccggaat
     4621 gccctgtttt gcaagggatt ctgagaattt gtaaaatgtg tgttttagct ggcatcttgg
     4681 caaaacagag caagatggag caagttattg cgggctgtta gtagtaaagt tggtagtaaa
     4741 acgaggtata cttactacta aggattattt tcaatctaat aataattctc cttctggata
     4801 atatgcactg cgatttgtcc aagtcatacc tatttgctcg tattcaatat gattctccga
     4861 aacatcctca tcaataaatt taacacactt gcatctcaaa tagtcggcac cacatttagt
     4921 acacttacta ttttttaccc acttaatcgg tgcacaaatt atcttattat ctttaagata
     4981 tgatgcttct tccttagaaa cagctaattc tactatttcc tctttccctt cccacctagt
     5041 tccagaaata tattttggat g
//
LOCUS       DAWDWH010000153         5034 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6789_length_5034_cov_12.585258, whole genome shotgun sequence.
ACCESSION   DAWDWH010000153 DAWDWH010000000
VERSION     DAWDWH010000153.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6789_length_5034_cov_12.585258"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1477 a          980 c         1350 g         1227 t
ORIGIN      
        1 gtacaagaac ttgtgagtag tatctgtttc gacagtacaa aaaaaagcat actcattgaa
       61 gtaggaatga aacataaagg tttataactt tttataagtt ttcagtaacg gaattgcgat
      121 ggaacaatat tcggtaaccg ggatgagctg tgccgcctgc agcagcagag tagaaaaagc
      181 agtcagtaaa gttccgggtg tgacttcctg ctcggtgagt ctgctgacaa attcgatggg
      241 cgtggaaggg acggcaactt gcgatgcgat catctcggcg gtggaagctg caggatatca
      301 tgcagaaaag aagggaacgg gcacaaaggg aaatcagggc agtgcggcaa aggatgatgc
      361 actgctgaaa gatacggaaa caccaaaact gaaaaaaagg ctgtttgctt cggttggttt
      421 tttactggta ctgatgtatt tctcgatggg gcatatgatg tggaactggc ctctgccatc
      481 tttctttgcg ggaaaccatg tggcgatggg actgcttcag ctgcttttga cggtcattat
      541 catggtcatc aaccagaaat tctttatcag tggttttaaa ggactgctga ataaatcccc
      601 gaacatggat acgctggtag ctcttggatc cggagcatcg tttctttaca gtgtctatgc
      661 actgtttgcg atgacggatg cccaggtaaa gggtgatatg gcagctgtca tggcgtatat
      721 gcatgaattt tatttcgaat ctgcggcgat gatcctgaca ctgatcacgg tcggcaaaat
      781 gctggaggca cgttcgaaag gaaagacaac ggatgcactg aagagtctga tgaaactggc
      841 accaaagaca gcagttctgc tgcaggacgg tcaggaagtg accgtttcca tcgaagaagt
      901 acaggcgggt gacatttttg tagtgcgtcc tggaaaaaat ataccggtgg atggtatcgt
      961 tctggaagga aacagtgcag taaatgaagc ggcactgacc ggtgagagta ttccggttga
     1021 caaagcagaa ggagataaaa tttctgcggc aacagtcaat cagtccggat ttatcaaatg
     1081 ccgtgcgacc agagtcggag aagataccac gctttcacag atcattcaga tggtcagtga
     1141 tgcggcagcc acaaaggcac cgatcgccaa gatcgcagat aaggtttccg gtgtgtttgt
     1201 accggcagtc attacgatcg cagtgatcac cttcatcgta tggatgctgg caggccagac
     1261 ctttggatat gcactggcaa gagccatttc cgttctggtt atcagctgcc cgtgtgccct
     1321 cggacttgcg accccggttg cgatcatggt cggaaatggt atgggtgcaa agcatggaat
     1381 catgtttaaa acagctgttt cgctggaaga gaccggaaaa atgcagatcg tagcactcga
     1441 taagaccggt acgatcacaa gcggagagcc gaaagtaacc gatatcattc cggcggaagg
     1501 cgtaagcgaa gaggagctgc ttcagatggc atttgctctg gaaaagaaaa gtgagcatcc
     1561 gctggcaaaa gcgatcctgc tggaggcaga gcggcaaaaa gtcagagcag aggaagtatc
     1621 ggattttcag gcacttccgg gaaatggtct gacagccagt cttcatggaa gccgcctctt
     1681 tggtggaaat atgaaattta tctccgaaat ctgcaaaatt tcagagaaac agaaacgcca
     1741 ggtggaagca ctggcagaag atggaaagac gccgctgttt tttgcaaaag aagaccgttt
     1801 gctcggtgtg atcgcagtag cggatgtgat caaagaagag agtgcaagag ccgtaaaaga
     1861 actgcagaac atgggaatcc gggtagtcat gctgaccggg gacaatgagc ggacagcaag
     1921 ggcgatcgga cggcaggcag gcgttgatga ggtcattgcc ggagtccttc cggaaggaaa
     1981 ggaaagtgtc atccgctctc tgaaagaaaa aggaaaggtt gtcatggtag gtgacggcat
     2041 caacgatgca ccggccctga caagggcaga tatgggaatt gcaatcggag ccggaacgga
     2101 tatcgcaatc gatgcggccg atgttgtgct gatgaagagt aagctggatg atgtaccggc
     2161 agcgatccgt ttgagccgtg cgacactgag aaatatccat gaaaatctgt tctgggcttt
     2221 tatttacaac atcatcggca ttcctctggc agccggaatc tggatcccgg tctttggatg
     2281 gcagttaaac ccgatgttcg gggcagcagc catgagcctt tcaagcttct gcgtggtaac
     2341 aaatgcactg cgtctgaatt tcttcggaat gtacgatgca aaaaaagata aaaaaataaa
     2401 aaatcaggtt acgttacaga ctgtgaatgc aaaatcacag atgcaaaata aatcaaagga
     2461 aaaggagaat catactatgg aaaaaacaat ggaaatcaaa ggaatgatgt gtggacactg
     2521 cgaggcaacc gtaaagaagg cactggaggc actgccacag gtagaggaag cgatcgtcag
     2581 ccacgaaaaa ggaactgcag ttgtaaagct gaatgcagag atcgcagatg agacactgaa
     2641 aaagaccgta gaggataaag actatcaggt agtgtctgta aagtaaggca taacgcatct
     2701 gtctgcaata tttgccgcat ggctgaacgg ctgcaaactt tacaaaaacc atacaaaagt
     2761 ttgctttaca attaaagaaa aaggaattat aatggttgag ggtctgtaaa gccctcaact
     2821 tttttcttaa atttcagtac aaaggcaggt gaacagaatg gacagttgtg acagtcatgg
     2881 gcgaagtaga agcataggat gggaagtgag cagcagtccg ttctgtctgg ttttctgatt
     2941 tcttatccta ttttccgact cccctggacc gttatgaaag ttcagtcatg tgcagaaaat
     3001 catttacagt tttgtttttc tgtatttttg tgtggctgaa cggttacgaa aacagaataa
     3061 ggaggatgag acattggaaa acaaagatta tacatcagag atcctgggta tcatcagaag
     3121 caatgcttca ccgggcgtta tgcggaatca gctcgaagat taccatgaga atgacctggc
     3181 agaggtgttc ccggatctta ccattgtaga aagaagaaag atctgtcgta tcctggatac
     3241 ggagatgctt tccgatattt ttgaacatat cgatgagaaa ccggcagcgg agtatctgga
     3301 tgagatggat gtcagaaagg cagcaaagat cctctccagt atggagaccg atgcggtgat
     3361 cgatgtcctg aagatgatcc cgaaggagaa aagagcactg ctgatcgagc tgaccgatgc
     3421 agatacaaga gaagatatcg ctgtgatcgc ttcttttgat gatgaagaga tcggaagcag
     3481 gatgacgacc aactttatca tgatccgcga gaatcttacg gtaaagcagg cgatgagcag
     3541 cctggtagaa caggcagcaa agaacgataa tatttctacc attttcatgg tgacggagga
     3601 tcagacgttt tacggagcca tggatttaaa ggatttgatc attgcaagac aggattctcc
     3661 gcttgaggat ctggttgtaa cttcttatcc atatgtatat ggtcatgagc tgatcgatga
     3721 ctgtattgaa aaactgaaag attattccga ggattctatc ccggtattaa ataataacaa
     3781 taaactactc ggtgttatca catcgcagag catcgtatca ctggtcgatg acgagatggg
     3841 cgaggattat gcgatgttcg ccggtctgac tgcggaggaa gatttaaaag agcctctgcg
     3901 tgagagtatc aaaaaacgtc tgccatggct gctcgttctg ttaggtcttg gtatggttgt
     3961 ttcttctgtt gtaggtatat ttgagacggt agtaagccag ctgacgatca tcatggcatt
     4021 ccagtcactg attctggata tggcaggtaa cgtcggtacg cagtcactgg ctgtaacgat
     4081 ccgtgtcctg atggatgaat ctctgaccgg caaacagaag ttccagcttg ttttgaaaga
     4141 aatgaagatc ggattctcga atggtatgat cctgggaacg atatcgtttg ctctggtggg
     4201 attatatatc gcactgttta aaggcaagac attcctgttt gcctatgcgg tgtccggatg
     4261 catcggcctc tctctggtac tggcgatggt cgtttccgga gcggttggta cctgtatccc
     4321 gctgtttttc aagaagatcc atgtagaccc ggccgttgcg tccggcccgc tgataacaac
     4381 gatcaacgac cttgtcgcag ttgtgtctta ttacggactg agctggattc ttctgatcaa
     4441 tatactgcat ttggcaggct aaattcaagt cgaaagaaaa aaagacaatt gcacagcggc
     4501 agttgtcttt tttttgacga aagataacat accatatcgc tatgttttga aggtattgac
     4561 aagttttctg ttttatgata ccataattaa aacctagtaa acatatgtga aatataaaga
     4621 taaggagtag ctatgaaatt atcaatgaaa agcagatatg catttcgggc attggcagat
     4681 ctgtcgatcc attccaaatc agcacatgtg cctctgaatg tccttgcaca gcgaaatggg
     4741 atatcgccgc agtttctgga gcaggttttc gcaagccttc gaagagccgg aattgtcaga
     4801 agcatcaaag gaccgcaggg aggctattat ctggccagac cggccagtga gatcaccgtc
     4861 agcgacattg tctatgcgat cgacggtgat tattatataa cagacgaaaa gacagccgat
     4921 ccctgtaaag atgaagaaat atccgatgtc atccaggagc tgatcatcga tccgctgaac
     4981 cggcagacga agaaagtact tcagggtgtc acactcgaag atatggagga agaa
//
LOCUS       DAWDWH010000154         4993 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6846_length_4993_cov_11.952410, whole genome shotgun sequence.
ACCESSION   DAWDWH010000154 DAWDWH010000000
VERSION     DAWDWH010000154.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6846_length_4993_cov_11.952410"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1939..1948
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1519 a          986 c         1310 g         1168 t
ORIGIN      
        1 ttctacggca ttaccccggt gagtccggat catgaagacg aaaagatgat gggacagatg
       61 gctgaattta tctgcaaagc aaactatggt ctgaaaaatg gctgttttga aatggattat
      121 aatgacggag aaattcgcta taagagctat gttgactgtg acggaatcat tccatcacag
      181 aaagtgatcg agaacagcat ttactgtatt gcgaatatgt atgagactta cgcaccggga
      241 atcatcgata ttcttttcaa agatgtaccg gcaaaagaag ctattgaaaa gtgtgaggat
      301 ggaaggagac ggcttcgtga actgctgcag gcaagaacgg gcaatacgga tgctcttctc
      361 gatattttgc agagaaagct tgcagaaatg gaggcacctc gcgttgatat cggtgaagat
      421 acggcagacg cagatgacga ggatcatgat gagatcgagt atgatgtgat ggaagatgac
      481 gaggactatg cgtttgaaga tgatgaagac gatgagtctg atgaggatga gatggaagac
      541 gatgagtttg atgatgacga aggaggcgag gattaagcag gtgatttggc agtgagcagc
      601 aaaccttccc attgacactg tggctgctat ctcatataat aacaatagaa aacagattac
      661 aaagaaagac agggcatggt ctgttgtcag ggtacaacag gccatgcttt tttgaaaggg
      721 aggtttttta tttatcatgt ccagtgactc gacttcattg gttattattc tggtctgtct
      781 tgttttgtcc gcatattttt cagcgaccga gacagccttt tccacgatga gccatgcacg
      841 tatgaaaaca atggcagaca agggtgacaa atccgcagca cttgcacttc agctggcaga
      901 caattacgac acgctgctct ctgcgatcct gatcggcaac aacctggtca atattctgct
      961 ctcatctgtg gcgacactgc tctgtgtcaa atttctgggt gagcagacag gggcgagcgt
     1021 tgcaacagct tccgtcacgg ttgtcgtgct gatctttggt gaaatctcgc cgaaaagcat
     1081 tgccaaagaa tcgccggaga aatttgccat gttttccgca ccgtttatcc gtgccttgat
     1141 ggtgctttta aaaccattta tctggatgtt cgctcaatgg aaaaagctgc ttgtcaaact
     1201 gttcaacaag ccttcggatg accgtgggat cacggacgag gagctgatga cgatcgtgga
     1261 agaagcagag caggaaggcg ggatcgatgc cgatgaaagt acactgatcc gcagtgcgat
     1321 tggttttatg gatcttgaga caagtgatat ttatacgccg cgtgtggaca ttgagagtat
     1381 tccgctggat gcgacaaaag aagaaatcgc aaaaatgttt ctggataccg gattttcaag
     1441 acttccggtt tacgaggaag atatcgacca tattgtcggc attgtcaacc agaaggattt
     1501 ccacaatttc atttatcata ccgaccgacc tctgaaagat atcatccgcc cggtgctgtt
     1561 tatcacaccg acgatgaaga tcggtgtcct gctgaaaaag cttcaggaca gtaaaactca
     1621 cattgcgatc attctggatg aatttggcgg aacggaaggt ctggttacga ttgaagatat
     1681 catcgaagag ctggtcggtg atatctggga tgagcatgac cgtgtcatca aagaaatcga
     1741 aaaaatttca gaccgggaat atctgatgag cggatcggca aatattgata aagtatttga
     1801 actgctggat atcgagaaag cggtggatgt caataccttg agcggctgga tcatggaaga
     1861 gctggggcag atcccgaaag agggggacag ttttgtaagt gacggcgtga aagtagttgt
     1921 cctgaagatg gatgaccann nnnnnnnnaa aggcgatgga atcgccaaat cgagcttaca
     1981 tggctgaact gttgatgttg attttattct tactaaataa acgaacagga ggattactat
     2041 gagacttacc ccgaaggaac aggaaaaact gatgcttcac atggcgggaa atcttgccaa
     2101 agagagaaaa gaacgcggcg tgaagctgaa ttatgtagag tcgcttgctt atatcagttc
     2161 ggaacttctg gaactggcac gtgacggaaa aacggttgtg gaactgatgc agcttggaac
     2221 gaagatcctt accaaaaaag atgttatgga tggtgttgca gatatgctgg aagaagtaca
     2281 ggtagaggca acttttccgg atggaaccaa gctggtaacg gtacataatc cgatccagta
     2341 gacagaggca gacccgtaga tgatttgcgg gcgaatcttg aagaaggagg acaacaaacc
     2401 atgaaaatcg gtgaaattat tccggcagac agagagatca cactcaacga aggaaaaaac
     2461 atagtgaaaa ttcttgttgc gaataaagga gaccgcccgg tgcaggtggg atctcattat
     2521 cattttttcg aagtaaataa atgcctttcg tttgaccgtg agacagctta tggatatcat
     2581 ctggacattc cgtcaggaac atcagttcgt tttgaaccgg gcgaagaaaa agaggttcag
     2641 ctcacacaga tgggcggcag aaagcgtgta tttggattaa atgacctgac aagagccaga
     2701 gcagaagaag acacgaaaaa agcttctatg gaaaaagcag cattaaaagg ttttgtatag
     2761 gaggatggca gatgagcgta aaaatttccg gtgaaaaata tgcaatgatg tacggcccga
     2821 ccgtgggcga taaagtaaga ctggcagata caagtcttgt cgttgaagtg gaaaaagatt
     2881 ttaccaccta tggagatgaa attaaattcg gaggcggaaa aaccatccgt gacggtatgg
     2941 gacagtctgt taagacctgc agcaaagacg gagatctgga tctggtcatc acaaatgcac
     3001 tgatcgtgga tgtgaccgga atcataaaag cagatatcgg tatcaaagac ggcaaaatcg
     3061 tcggaatcgg aaaagcagga aacccggata ttatggacgg cgtaactccg ggcatgacgg
     3121 ttggagcttc cacagaggca ctggcaggag aaggcatgat cgtaactgcc ggcggaattg
     3181 atacccacat tcattttatc agcccgcagc aggttgactg tgcactgtac agcggtgtga
     3241 caacgatgat tggaggcggt accggtccgg cagacggaac caatgcaacg acctgtacac
     3301 ctggaccgtg gaacctgaaa atgatgctca aagcagcaga agaatatccg atgaacttgg
     3361 gattcctcgg aaaaggaaac tgttcggatg aagcaccgct gatcgagcag gtcaaagcag
     3421 gtgcaatggg actgaaaatt catgaagact ggggtgcgac accggctgtt atcaaccact
     3481 gcctgaatgt agcagatgag tacgatgtgc aggtagcaat ccataccgat acgctgaatg
     3541 agggcggatg tgtcgaagat accctggcag cgatcggagg ccgaacgatc catacttacc
     3601 acaccgaggg tgcaggcgga ggacacgcac cggacatcat tcgtgcagcg gcagcaggaa
     3661 atgtacttcc gtcatccaca aacccgacca tgccatacac ggtcaataca ctggatgagc
     3721 atctggacat gctgatggta tgccatcatc tggacaaaaa aatcccggaa gatgtcgcat
     3781 ttgcagattc cagaatccgt ccggaaacca tcgcagcaga agatgttctg catgacatgg
     3841 gtatcttcag tatgatgagt tcagactctc aggcaatggg acgtgtcggc gaagttatca
     3901 cacgtacctg gcagacagca agcaaaatga aagacgaacg cggagcactt ccggaagacg
     3961 aaggcaaagg aaacgacaac ttccgtgtaa aacgttatat cgcaaaatat acgatcaatc
     4021 cggcactgac acatggtatt gcagattatg taggttctgt ggaaaaaggc aagttcgcag
     4081 acctggtact gtggaaaccg gcattcttcg gtgcaaaacc ggatatgatc atcaaaggcg
     4141 gcatgatcat tgcatctaag atgggtgatg caaatgcatc catcccgacc acacagccag
     4201 ttctctatca gccgatgttc gcagcacatg gaaaggcaaa aaatgaagca tgcctgacct
     4261 ttgtctccaa agcagcttac gatgcagata tcaaagatgc atacggactg gaaaaaaccg
     4321 ttgttccggt aaaaggatgc cgcacgatcg ccaagaaaga catggtattt aataatcgca
     4381 caccgaagat caccgtagat ccggaaacct atgaagtgac ggtcgacgga gaatccatca
     4441 catccaaacc ggcacaaaaa ctgccgctta cacagctgta taacttattt tgatgtccaa
     4501 gggagaaatg gacataaaat ggatgcttgc atacattttt atgtctatga atcccgccaa
     4561 atcataaaaa agcagccatt tttcgcagaa aaatgagcgg attttgaatg attgcaaaat
     4621 tgcgagagtt atattataac ggagcaattt tgaggacatc aatttaaata aagagttagg
     4681 aggaacaaaa tgttaggagt ttgcctttta tttgtgggaa ttgtactgat caacaacggc
     4741 atgtgtacgc tgtacaaagt cgatggcaaa tcggcggcag tgatgaattt gctgaccgga
     4801 ggcctgtcgg tctttatcaa tttcgtgagc ctggtacagg gtaattatta tgcggcagga
     4861 accggacttc tgtttggatt tacctatctg tttgttgcat tcagcaagat ttacaagctc
     4921 agcccgattc cgtttgcatg gttttccacc tttgttgcag tcaatgcact gatctttgga
     4981 accatcgagg gaa
//
LOCUS       DAWDWH010000155         4984 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6865_length_4984_cov_14.115236, whole genome shotgun sequence.
ACCESSION   DAWDWH010000155 DAWDWH010000000
VERSION     DAWDWH010000155.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6865_length_4984_cov_14.115236"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1201 a         1301 c          982 g         1500 t
ORIGIN      
        1 ctacatatac tcttgcgata gatttttttc ttcttcctgt accgtagaat ttgttagcca
       61 ctgtcattta cctcctgtta aaatgttaat acttctggct tctgagctgc atgtttgtga
      121 tccggaccag catatacaaa aagttttttg tacatttctc ttcccagagg tccttttgga
      181 agcatacctt taactgccag ttcgataact ctttccggtt tcttgtttaa catttctttc
      241 agagttgtct ctttcattcc tccaacgtag tcggaatgtc tgtagtaaac tttctgttcc
      301 agttttttac ctgttacttt gattttgtct gcgtttgtga tgattacata atcacctgta
      361 tcgataaacg gagtaaagat cggtttgttt ttacctctta agatctttgc tacttctgat
      421 gctaatctac ctaatgtata tcctgttgcg tccactacat accattttct ttcgattgtt
      481 gctggactag ccatataact tttcatgggg taacctcctg attgatattc cattatgagt
      541 ttctatagta aaatgtcgtt accggggctt ggatcagaca tttacatact tcgtcacatt
      601 gcttaattat attatacaga cccgtgtgtg tcaacctttt tttacatttt ttttacgatt
      661 cttatgaacc ttttttacga ttgtaagtct gcaaagcacg gaatgatcca ccggtatcgc
      721 agttttaatt tttaacctca acatcttaac acttaaaaga agattttccg cacatcattg
      781 aacatcacca ctaccatcaa aagcatcaga agcatcagtc cgacaaaatg taccattcct
      841 tctttctcgg gatcgatccg tttcccgcgg atcagctcaa tgatcagaaa gaccagtctg
      901 ccgccgtcca gtgccgggat tggcagaagg ttcatgaccc ccaggtttgc tgtcagaagg
      961 atcgatatgt tcagcatatt cagccataca tagaaacctc cggaggactg tgactcctga
     1021 tacgtctctc cgatgtaact cacaacaccg accggaccgg acatatcatt gatgctcgcc
     1081 tgaccggtaa agagcatttt aatactctcg attgtcagct gaatccaata ttttacttca
     1141 taggcactgt atttcagggt gccgaggata ccgcattttt cgcggtaatt ggaactgcag
     1201 ctgataccga aaatgtagcg accgctctca tttgcaaccg gatccatctg aacggtgata
     1261 tctttgccgt cgcgttctac cgtcatcgtc acggactgtt tctgatgaaa ggtcacatag
     1321 ttggttactt ctcttgccac atggatattg ctgtgattga tctttttaac aatatcgcct
     1381 tcttttaggc ctgcttctgc tgccgggtat ccgtcactca cctgcaggat caccggttta
     1441 tcgtacccga tacttcctat aataaacaga gacagtacaa atgccaggat aaagttaaat
     1501 accggacctg ctgcaataac tgaaatcctt gcccatacgg atgccgctgc aaatgctccc
     1561 ggttccatcg ttccttcatc ttctccaagc atcatgcagg aaccgccaaa cggcagaagt
     1621 ttccaggaat attttgttcc tcctttgtca aagcttaaca gtctcggtcc cattcccaga
     1681 gaaaattcca caaccgtaat gccatttttc ttcgccagca gaaaatgtcc gaattcgtga
     1741 aaaagaacaa tcaggctgaa gatcaacaat gctataatta tgctcaattt accacctgct
     1801 ttcaatcatt tcataagttt cctgttcaca ctgcaggatc tgttccaccg ttggagcttc
     1861 aatcactttg tgtgcatcca tacaggtttt gatgatctcg taaatatcca gaaaaccgat
     1921 cttgcggttc aaaaatttgc tgactgcacg ctcgtttgcc gcattaaata cagtcggcat
     1981 ggagccgcct gcttttgccg ctgcaaaggc cagcggaagt ccttcgaaat tctccatatc
     2041 cggtttttcg aaggtgatct gtgacaggct ccagaaatcc agacgttctc cggccagata
     2101 ttcccgatct ggatagaaca gtgcatactg gatcggaagc ttcatatccg gggttccaag
     2161 ctgggcgatc accgcaccgt ctttgtactg caccatggaa tggatgatgc tctgcggctg
     2221 gatcacaacc tggatctgat ccagttccac atcaaacagc cattttgcct ccatcacttc
     2281 cagtcctttg tttaccatac tcgcagaatc gatcgtgatc ttatgtccca tcgaccagtt
     2341 tggatgtttc agtgcatcct caagctgcac atgtttcaga aatgctttat cttttccgcg
     2401 gaacggaccg ccggatgctg tcagcaggat tttgtcgatc tgtgttttct tttctccgtt
     2461 taaggactgg aaaatcgcac tgtgctcgct gtccaccgga aggatcttta cgttgtattc
     2521 ttttgccaga ggcataatga tatgaccggc agttacaagt gtctctttgt ttgcaagtgc
     2581 aatgtccttt cctgctttta ttgctgcaat ggtcgggcgt atcccgatca tgccaacgat
     2641 cgctgtcacc agcacttctg cttcttccat ttgtgcgatc tcaagaagtc cctccatgcc
     2701 ggaaacgatc ttcaccggaa ggtctgcggt acagatccgc agctcctttg ctttttcttc
     2761 gtcccagaca gctgcaatct gcggatgaaa ctcgcggatc tggctctcca gctgtgtaat
     2821 attatgacct gctgccagtg cttttacgca gagcctttcc ggattggcac gcaccacatc
     2881 cagcgtctgc gttccgatcg atccggttga acccaaaact gctatatttt tcatttgttc
     2941 ctccttatct catcataaac atggaaagat aataaataac cggtgcagta aagatcacac
     3001 tgtcaaaacg atccatgatc ccgccatgtc ccggtatcag tgtaccgtag tctttgatgt
     3061 tgtgattacg cttgatcgct gatgctgcca gatctccgac catggagatc agtgctccga
     3121 ctgcacagat aatcgcataa gtgatcaccg ggttttctgc ctccagatga ctgcctacga
     3181 ctgccgcata gatggctccc agaagtgccg caccgacaac accgccgacg gctccttcca
     3241 cggatttctt cgggcttaaa accggagaca ttttgtgttt cccgatcagc atgccgacac
     3301 agtaagcaca ggtatcacat ccccaagaac acagaaagat cagccataca agaaatgctc
     3361 cgtcgcggat catcctggtc tgataaatgt aggacagcat gacacccaca tagaccacac
     3421 cgaaaaaggc tgccatcacc tgttctgcat gatatttcgg ataagaaaac acatagacaa
     3481 acatcatggc gatcagcaca atgatcaagt tcatcatcgc atactccgaa tatccgagac
     3541 tcagcagaat ataatgtaag atcacgccca gatatcccac cactgccagc aggttttcac
     3601 cctgtttctg taccttcact gcacgataca gttcaaacat accgatcagc gatacgatgg
     3661 tcagtgttcc gtacaaaatc cagcctccgc tgcagattgt cagaagtgcc actgccacca
     3721 gaacgatacc gctgataagt cttgtcacaa acatatctta ttctcctttc acaccgccat
     3781 atctgcgttc tcttccatta tatttctcaa tggcctttaa cagttcttct ttattgaatg
     3841 ccggccacgg aacttccgtg aaatagaact ccgaataagc cagctgccac agcagatagt
     3901 tggacagacg cagctcaccg ctggtgcgaa tcagaagatc cggatccggt atctctctcg
     3961 tatccagata tgcagagaag agatcttctg tgatgtcttc cggttttact gccccgtcta
     4021 cgcagtcctg gctgagctga cggatcgcac gcacgatctc atcacggctt ccatagttga
     4081 tcgcaaggtt aaaattcagc ccggtattgt ttttggaaga ctcaaccagt tcttttaagc
     4141 tctcctgtag atcatctgca agtgccgtcg gatctccgag aacacggact ttcatattat
     4201 tttcccttgc tgtcttgata caggtctttg tatagcttcg gaaaagcttc atcagcccgt
     4261 caacttcttc tctggaacgt ttccagttct cagtcgaaaa cagatagacc gtcagatatt
     4321 tgatgccgat atcataagcg tcacggcaga ttttttccag attcttggca ccgcgtacat
     4381 gcccgtaatt acgcggcatc cctttgctct ttgcccagcg tccgttgccg tccagaatga
     4441 ttgctacatg ttgtggaata ttcatacaca aaacctcctg tgtgcaaaaa agcagggatg
     4501 ccacactgca acatccctgt atttacccaa tatttacctt ctaagactac acggtcatga
     4561 tctcttttga tttctcctcg atagccttgt cgatatcctt gatatacttg tctgtcagct
     4621 tctggacatt ttcttccagc tctttgactt cgtcctcgga tacctctttt ccctttccaa
     4681 gtttcttgaa tgcatcgttt gcgtcacgtc tgatattgcg gacagcaact tttgcaccct
     4741 cgcctttttt cttcacttct tttaccagat cttttcttcg ttcctctgtc agttccggga
     4801 ataccagacg gatcactttt ccgtcggtat tcggattgat gccaaggtcg gatgtctgga
     4861 tcgctttgca gatctctttg atcagagatg catcccacgg ctggatctgg atcatacgcg
     4921 gctccggaac ggtaacgttt cctacctgct gaagtggcgt cggtgttccg tagtactcta
     4981 cggt
//
LOCUS       DAWDWH010000156         4954 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6898_length_4954_cov_11.886099, whole genome shotgun sequence.
ACCESSION   DAWDWH010000156 DAWDWH010000000
VERSION     DAWDWH010000156.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6898_length_4954_cov_11.886099"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4732..4741
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1132 a         1289 c          970 g         1553 t
ORIGIN      
        1 gatcgtatca tccggtctct ttttcttcat ccgaagctcc cctcacaaat gctgtcttat
       61 ttttatatgc atatgttttt ggattgatgc atacgatacg caaaaagagg aattttttat
      121 gaaatctttc ttttcttcac ttcttggtgc taccctgttt gtgattctgc tgtttttccc
      181 tggtctggtc tttgaaggtg caaaaatggg actgaacctg tggtttttca ctctggttcc
      241 cacccttctg ccattcatgg tcttttccaa catcctgatc cagagccgcc tgatcgaaca
      301 tcttctcttc ctgcccggac tttttacccg aaaatatctc catctctccc cggccgggct
      361 ttatgcgttc ctgtgcggct ttttcttcgg ttatccttcc ggtgccaaaa ttcttgccga
      421 tcttttaaaa accgatcaga tcgatccaaa ggaagcttcc atgctcttcg gtttctgcaa
      481 caacgtaagt ccggcttttc ttatcaccta ttttctgaca ctgcacttaa aacacccgga
      541 gaaagccggg atcaccgtct cttttctgta cataataccc gcttttgtct gtctgatggc
      601 attttccttt tgcggcaatt ccttttctct ccgtgaaaaa aagacatcga gtcccgtgct
      661 ttctcttgca ctgcttgatg cctgcctcca agatgccctc tcagtcctgt taaagcttgg
      721 cggttatatt atattatttt ccgtcttatc caatgtgatc acacacatac cccgcatgcg
      781 tgcagaaagt gccgcttttt tctcctgctt tctggaaatt acaggcggaa tccctgccgt
      841 cactgccgca tttgcttatc cgcaaagtta tgtgatattg ctcccgtttc tggcttttgg
      901 cggtctttgc tctttcatgc aaacagggag tgtcataaaa gacaccccct tatctcttcg
      961 tagctatttt tttaccaaaa tactgctcgc tctgctgata tttctgtgtg aaatactctg
     1021 cattacgctg cttccgggcc ttttttaagc ctcgtcgtca tcgtaatccg catcctgctg
     1081 cggtgctgca taagatgcag ggtttccgcc cggtgacagc tcgcgtctgt tcttgtttac
     1141 gatatcgtag caagactgga tcgaattgat gaaagtattg tatttctcgc ctgctgcatc
     1201 gatcgtatgg ctcatgatct tttccagatt tgccagcatc tcatcggtgt actgcagtgc
     1261 acccatacgg atgttgttgg catcggttgt tgcattgtcc atgatctcct gtgcctgctg
     1321 gttggtgttc tccagcagct cattggactg tgcatatgcc tgctgcataa cttcactttc
     1381 cgtaaccatc tgctgtgcct gtgtctttgc gtctgcgatg atcgcatctg ctttggactg
     1441 tgcgtctgcc aggatcgcat ctttgttgct gatgatcttc tggtaacgtt tgatctcatc
     1501 cggtgtcttc atacgaagct cacgcagaag ctcttccagt tcttctttat ttaccacgat
     1561 ctttgtagaa gagagtggct ggaatttaca actgtctaca tactcctcga tttcttcaat
     1621 aatctgctca attctgctgc tcatcgttat cactcctatt ttttattctg atttctggtt
     1681 ctagttctaa tttaaagatg ccttacggat tgttttgtac ttcgcacttt ctcaatcctg
     1741 ctttgacact tccatttttt gatatatctt ctcaatcacc gactccggca caaatttgga
     1801 gatatcgcca tggtagctgg cgatctcttt gaccgttgtc gaacttaagt aggcatattc
     1861 cagactggtc gataagaaaa tcgtatcaat atctgagcgc aggatgcggt tggtctgcga
     1921 catctgcagt tcgtaatcaa aatcggtgat cgcccgcaga cccctcacca taaactgtgc
     1981 tccgcagttc cttgcaaaat ctacggacag accatcaaat gcctttattg tcacatttcc
     2041 gatatccttg gtgacatcag ctaatatatt aacacgttct tcggcagaaa acaacggcgt
     2101 ttttgcacta tttgtcagca caccgacgat cagttcatcc accagaacag cggaacgacg
     2161 gatgatatca agatgtccga acgtaaccgg atcaaaactt ccagggtaga cagctcttct
     2221 cataactcct ttttctcctt ctctataaac atatgaacat ttgtcttata ttctttgcgt
     2281 ttcagtacac ggaacccaaa ttccttaaga taggaaaaat cagtctccag agatgcctct
     2341 ataatgatca gggtacgctc atctgccact ttgctgtctt ttaagcagga aaggactcgc
     2401 ttttccagct cctggttgta aggagggtcc ataaaaatga caccgaagct ctccttgccg
     2461 tcctcgatct cacgtctcag acgatccagt gcagcaaaaa catctgactg cacaacaaaa
     2521 gcatccgcgt ccaacctagt aaacttcagg ttttcgcgga tgcaacacac tgcggcagga
     2581 tttttttcca caaagactgc ttcctttgct cctctgctga gtgcctcaat ccctatcgca
     2641 ccgctcccag aaaacagatc cagaaaacgg ataccgtata gttcatcctg tatcatatta
     2701 aaaagcgttt ctttaatccg gtccgtggtc ggtctggtct ccattccggg aaccgtctta
     2761 agtgccagtc tcctggcttt gcctgcgata actctcataa tttcttcctt tgttctgtgt
     2821 tttttctatt ataaaggttt tcacacttca cgtcaagccg aaaaaacagc aaaacaacgg
     2881 aagcaagcag catttgtgag cttatgtatc aaaggattct ttcgtttttg atgcttccgg
     2941 tctctttaaa tacttcccac tctgagatat tgactgcatg gtcaccgata cgttccagat
     3001 attttgcaac catcagaaga tccacacagg catcggtgtc ttttttgcct tctttcaggc
     3061 aggcaaccag ttcctctttc acctggttga aaaggtcatc aatcttatcg tctcttttat
     3121 aaaattcttt tgcctgctct ttatcccttg ccataaaaag ctctacccca tcacagagga
     3181 tctttctggt ctcttcgagc atctccggca tggacggtgc atactctttg tagctgatac
     3241 cgttcagacg cactgccata tctgcgatat ccgctgcatg gtcaccgata cgttccatgt
     3301 caccgactgc tttcataaca gaagatacga tacgcaaatc acttgccagc accggctgct
     3361 gcctggtcag cagcagcagg cattctgctt caatcttacg ctccatctca ttgatcttac
     3421 ggtcattttt ctgtattctg tccaattctt ccagatcatt aagtgcaatc gctgagagca
     3481 gatctgcaaa agcctgctga atcccctttc ccatggcatc catgttttcg cgaagagtct
     3541 ccagttcgtg ttcaaaattc attctcactg acattgtttt tcccccaatc aaccaaaacg
     3601 tcctgtaata taatcttcgg tcctcttatc ctgcggtctc gcaaagatgt catcggtatg
     3661 gttaaactcg atcacttctc ctaccaggaa gaatgcggtg tcatccgata cacgtgctgc
     3721 ctgctgcatg ttatgggtga caacaacaac cgtatatttt ttcttcagct ctaccatcag
     3781 ctcttcaatt tttaaagtag agatcggatc aagtgccgat gtcggctcat ccatcagaag
     3841 cacgtccggc tgtacggcca gtgctcttgc aatacagaga cgctgctgct gtccgccgga
     3901 aagccccagt gctgattttt tcaggcggtc ttttacctca tcaaagatcg ctgctccacg
     3961 cagactctct tccacaatct gatcgagtgt cgctttatct ttaatgccat ggatcctcgg
     4021 tccataagcg atattatcat aaatgctcat cgggaatgga tttgcctgct gaaataccat
     4081 acccactttt tttcgaagca gtgtggtatc tgccttcgga ctgtagatat cttctccgtc
     4141 aatcaggact tttccttcta tttttacgtt ctcaaccagg tcattcatac ggtttaagca
     4201 cttcagaaag gtggatttac cgcatccgga cggaccgata aatgcagtga tcgcattttc
     4261 ccgaatctcc atattgacat gttttaacgc atgattcgtt ccgtagtgca ggttcagatc
     4321 ctgcacactg atttttgtat tttccatggt gatgctcctc tttcttttat ttcatccgcg
     4381 ttacatcgaa acgcttcgcc agatattttg tcaggaaatt gatgcacagt acgatcacaa
     4441 gaagtacaaa tgcaattcca aacgctacat tatattctgc ttttgagctg ctaagataca
     4501 gctggatcgt caaagttccg cccggctgca ggatcttgga aagccatcct cccgctttcg
     4561 ggagcagata accgcttcct gccgtaaaga gcagggctgc cgattctccg acgatacgtc
     4621 cgatcgccag aatcactccg gtgatgatgc cgggcatggc agacggaagc aggattgtac
     4681 ggatcatgtg ccattttgtt gctcccatgc caagtgctcc gctccggtaa gnnnnnnnnn
     4741 ntcctgcgtg tttcgcgtga tcagtggcag gatcataagg ctcaaagtca gagcaccggt
     4801 cagcagagaa taaccaagtt tcagcgttgt tccaaaaaac acgcttccga aaagtccgaa
     4861 aataatcgat gggataccgg aaagtgtctc agtcgtaaat tcaatgatcc ttacgatctt
     4921 gcccggtttg gcatactcat tcagataaat ggca
//
LOCUS       DAWDWH010000157         4944 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6911_length_4944_cov_13.058908, whole genome shotgun sequence.
ACCESSION   DAWDWH010000157 DAWDWH010000000
VERSION     DAWDWH010000157.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6911_length_4944_cov_13.058908"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4536..4545
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1111 a         1336 c         1029 g         1458 t
ORIGIN      
        1 cgtctgggaa agatcccacg ggcagccgcc ctctttcttt cgaagcattt ccgtgatctg
       61 acagaaatca tcaaaatcat atgtttttgc cattttacag catttcctgg atcatagcta
      121 caacctgttt accaagagct gcggattttg catctgcatc ttccagggat gtgcccttta
      181 cgccatagta gaattttact ttcggctcag ttccggacgg tctcacgcac aaccatgctt
      241 catcactcag atcataatac agaacatttg aggacggaag accggtcttt gtcatttcgc
      301 cgctttccat gtttttgatg gtatctttct tgtaatctct tgcagaaagc actttgtagc
      361 ctgcgatctc ggacggtgtc tcatttctga gggtctccat gatctcctgg atcttctgaa
      421 gtccttcgat acctttcagt gtgatcgcct ggatgtcatc tttgtagtag ccgtatttct
      481 cgtacatatc gatcatggca tcccacagtg tcttaccttg tgtcttatag taagctgctg
      541 cttcacacag agccatcgtt gcaacgatcg catctttgtc tcttgcatgt gttccgatca
      601 ggcagccata gctctcctcg aatccgaaga ggtaggttcc ttttccactc tgttcaaaac
      661 caaggatctg ctgtccgatg tatttgaatc cggtcaggac ttcgatgaaa cgcagtccgt
      721 aggatctagc gatcgttcct gccatgtttg tagtaacgat cgtgctgact actgctccgt
      781 cttccggaag gctgccgttt gctgcctttc tctggctcag ctcgtattct tcaagcaggc
      841 atccggacat atttccggtc agagtatggt atacaccatc tttgtctttg acatgcacac
      901 cgagacggtc tgcgtccgga tcggttgcca gaaccaggtc tgcatcgact tcttttgcaa
      961 gtttcagccc cagttcgaaa gcttcttctg cttccgggtt cggataactt actgtcggga
     1021 aatcgccgtc cggcagttcc tgctctttta ctacatatac atgctcaaag ccaagctctt
     1081 ttaagacgcg tcttgccggg atattgccgg taccgtgcag cggtgtgtaa acgatcttga
     1141 gatctttgct gacttcacgg atggaatccc agtggatcac ctggctcttc agtgcttcaa
     1201 tatatggatc atcgatgttc gcaccgatca cttcgtaaag tcctgctgtg actgctgcat
     1261 ctttgtccat tgtttttacg tttgcatagt cggtaactgc ttttacttca gccatgatac
     1321 cggtgtcgtg aggcggtgtg atctgtgctc cgtcctccca gtaaacttta tagccattgt
     1381 attccggcgg attatgactt gctgtgacat tgatacctgc cgtacatttc agtgtacgca
     1441 cagcaaagga cagctccgga gtcggtctca gtgactcaaa cacataagcc ttgatgccgt
     1501 ttgcagccag gcacagtgct gcttcatctg caaattccgg tgacatatgt ctggaatcgt
     1561 aagcgatcgc tacgcctttt tcctgtgctc ctactgactt gatatagttt gcaagtccct
     1621 gtgtagcctt acgcacagtg taaacgttca tacggtttgt acctgccccg atgatacctc
     1681 ttaaaccggc agtaccaaac tccagatccg cataaaagcg ttctttgatt tctttttcgt
     1741 ctccctcaat tgctcgcagt tcttcctttg tagcttcgtc aaagtatgga tttgcgagcc
     1801 actcttcata gaccttttta taatccattg tgctacctcc attaaaatat tcaataggtg
     1861 gctatattat ataataaaaa ggtctaaaaa gaaacctcta atgcagaaac tttacaaatt
     1921 ctcgccgttt tttttcctgc atatcgagaa tttccagctt ctgaaggctt ttttccggta
     1981 tccaggtttt gcggctgttg aaataatggt tggcaagctt ccagaatttt tccggatagg
     2041 cgagtcttat acaaaaattt tcaaattctg catctccaag cggacgcact tcctgatatg
     2101 catggatcat ctccatgcca agcttctgat tccagccctg cttttccatg atcttgcgga
     2161 aaaactgata aaaatcggag atctgtacat caaaacagca tctcgcaaaa ttgactgcca
     2221 ccgctccttc ccggtcaaaa aacaggttat gctgactgta atccccatgg cagacccgtc
     2281 gctcccgtat acactgccgg tgcagagcat atgaactgct ggcacatacc cgtctggtta
     2341 cttcttctgc ctgagccata aattccggat acagttccag gaaacggctc tcaaaagcgg
     2401 tctttttctt tcgtgtccgc atgaaattcc gcaccttttt cagctctttt gtgtgccgct
     2461 cgatctcctc gtcaagcggc tctgctatat actctaaatt ttttctgatt tttttctctt
     2521 ctgccggatc tgcctcatcc gtctgtgccg ccgccatctg aaattcctgt atttccggca
     2581 gacgcaggac attgtgcagc cttgcaagat aatgcaccgc ccctacaata tcttcccggc
     2641 ttttcgtatc acactcccgc ccggaaaacc attttttgac cacgtacccg ttgccgtagc
     2701 ggtcagaagt gaccagattt ccttctgtat tttccaggag cagatcgcag cgtatccctc
     2761 tttctgtcag aaaaaaaagc actctgttct gaaaaaacag ccgctcctgc gaaccggaaa
     2821 attcagtcac ctgaacaagc cctctgtccg tatcacaaag aaaggagcca cgcccccgcc
     2881 ttgtgctttg cacatgcagg tcgtactgct ccaaaatcac cagatctttt tcattcactt
     2941 ttgatccccc cacaattcct ctcaataaaa tcagctatta agatcaaaaa tgccacacga
     3001 attgttcctt acttcgtact ctcacaattc tggcacctaa agctactcta tcttatgaaa
     3061 tgcagggggc aaatatacgg gaagttatcc ttcggtattt cgcagttctc tgctttttcc
     3121 gatcagccat tcccgctcat ttcgctgcgg ctcctctaat accagctcca gcagctttgc
     3181 aaggatctcg cccatctctt tgcccggctt catgccgtct tcgatcagat cttttccgct
     3241 gatcttcaga tctttgatgc aaaggcattg ctgtttctct aaaatggtgt gatacagggc
     3301 tgtcagctct tttaaataag ctttcttctc cggctgccac ttcggggact gagccagggt
     3361 atctgccatt ttcagtttca gaacctctgc aaaagcgtct gccccgactt tgctcactgc
     3421 tcttcgcacc gatttttcgg tcagttccgg ccgcagatcg tggtatcgca cataaacttt
     3481 gactttattt aaagtgtcgt tgtcgaattt aagacgatgc atgatattat ttgcaagctc
     3541 ggcactgatc tttgcatgcc cgtgaaaatg gtcgatcccg tctgcgtccg tggtatgtgt
     3601 ctgcggcttg ccgaaatcgt gaaacaatgc cgccagacgc agcgttttgt ctgcctctat
     3661 gttccttaag gcttccagcg tatgttctcc gacgttatag cagtgatgcg ggttattctg
     3721 cggtgtctcc atcgtgcggt caaactccgg cagaacaact gccgtgatgc cgtactccca
     3781 ggcatacctc agataatccg gatgcgggga aaccagcagt ttgaccagct ccgtctgaat
     3841 acgctcagca ctgatctttg agagcgtccc cgccagcttt ttcgccgctt ctgccgtgtc
     3901 cggatcgacc gtaaatccga gctgtgccga aaaacggaca gcacgcatga tacgcagtgc
     3961 atcttccccg aaacgttctt cggcattgcc gacgcaacgg atcactttct gctcgatgtc
     4021 cgctcttccg ccgaacacgt ccacaagacc ctcgctgtca ttatacgcca tggcgttgat
     4081 cgtaaaatca cggcgacgca ggtcttcttc cagatttgcc gtaaaagtca cttcttttgg
     4141 atgtcttgca tcctcatatt ctccatccac acgataggtc gtcacttcat aaccttcttt
     4201 tcccatcaga acggttaccg ttccgtgctg gatcccggta tcgaccgtgc gtgggaacag
     4261 ttttttgacc tgctgcggtg tcgcagaagt cgtgatatcc cagtcatttg gtatccgtcc
     4321 gagaatcgaa tcacgcacgc agccgccgac cgcatagcct tcatatccat gttgttccag
     4381 tgtttcgatg atctgtttca catcatccgg aagtgtgatc ttcattctct tatttcccct
     4441 ttctgttatt ctgtctgtaa tttttccagg tactttcaca gtctggacgt aaaatccacg
     4501 gaactgtttt ctgcaattcc acctgtctct gtctcnnnnn nnnnnttacc ggaatgtaaa
     4561 aaacaaaatc accaggatac caagtacgat acggtaccag ccgaaaatct tgaaatcgtg
     4621 ctttcttatg taattcatca gaaatttgat caccagaacc gatacaacaa atgacactgc
     4681 cataccgacc agcaggatca tcatttctgc ccctgagaaa gccagaccaa atttcaggat
     4741 ctttaaaagg ctggctcctg ccatgaccgg aacggccagg aaaaaggtaa actctgctgc
     4801 ggttgttctg gaaagtccga tcaggatacc gccaagaatg gtcgcaccgg atctggaagt
     4861 tcccggaaaa atggccgcga tcagctggaa aagtccgacc agcatcgcat cccggtatgt
     4921 gatctgggca atactggtga tgcg
//
LOCUS       DAWDWH010000158         4942 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_6915_length_4942_cov_8.886024, whole genome shotgun sequence.
ACCESSION   DAWDWH010000158 DAWDWH010000000
VERSION     DAWDWH010000158.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6915_length_4942_cov_8.886024"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1002..1011
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1728..1737
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1296 a         1328 c          943 g         1355 t
ORIGIN      
        1 agaatttttt catttttaag gcaaaaaaat gttatttcac cacacttttc cgtagaaacg
       61 gccgggcagt tcgccttttc taaccgttct atggtttccg gtgccggatg cccgtagcga
      121 tttccttttc cataagacaa aaccgccatc tgtggtttca ggatatccag cagttcttct
      181 gaggtggctc ctttggaacc atgatgtcct gcctttaaaa cttcgatttt atttcctcct
      241 tccagaagct gcggcagttt ttttcttctg atcagatcct tttccccttc tttttccaga
      301 tcccccatca gcatcacatc aaaagatttg tattttacat gtagcaccat ggatgtctcg
      361 tttttatttt tggaagtatc cccagctttc ggggcaagac aggtaagcat catcgaccgg
      421 gtacagatac agtcaccttc ccgcattttg tgcacggcga tcttctgccc tgctgccagt
      481 ctttcaatct caaggtacgt ctcatccgga tttttgatgg ctggcaatac cagatttttt
      541 attttaatac tgcatgtttg acgctccagc agttccctta ctccattgat atgatccata
      601 tccatgtgac ttaaaaacac ataatccaac cggtcaatcc catgataccg caaatacgga
      661 agaattctgt attttccaac gttcgagaca ctgctgcttc cgccgtcaca caacatggtc
      721 acaccggtat catcccttag cacaatgcca tccccctgtc cgacatccag aaaggtcaca
      781 gaaaacgcct tcggctccgt ccgcttcaaa aagaaaatcg ccatgaaaac aaagaccaga
      841 taacagactt tcttttgttt tttctgaagc agccacacag cccccgcaga caggatcaca
      901 taatacagaa gaatcgacca cagtttcggt cttccaaaaa tcatctgaga aaaaggcagt
      961 tccagagata cgcggcacag acaggaatat aaatttaaca cnnnnnnnnn nactattcaa
     1021 catcacactg taaagaggaa aagaaaaaga atgccacaga acacacggca gcataaaaag
     1081 ctgaatagcc gccccggaag agatatatga tccgtaatgc tttttcagaa tcggataaaa
     1141 tccaccgatc gctgccacag cagaaactga catctgaaat ccagatgaga ataataaaat
     1201 gggataatca aaaagaagta aaaaaacagc tgctgacaga gcactcaata aatcatacgt
     1261 ccttccaagg cactgtgctc cgagaaaaag aagaaacatg atcatggcac ggcgtgtaga
     1321 tacacctgca ccggtaaaaa ctgcataaaa taacataaaa ctgcctgcga tcccggaaga
     1381 aaccacaaat gagatcccca gttttcgcaa gatatgatag agtgccatgc caaacagaga
     1441 aatatgaagt ccggaaatcg caagcacatg agcaatgccg cccacctgat accagtctct
     1501 gatctcctca tccagcctgc tcttatcgcc aagtagcatc gctgcgagaa ttccggcatc
     1561 ctgctcatca aatatctgct ccagatgccg catacagatc ctgcggactt catacatcca
     1621 ctgtaacagc ggattctgcc tgccatcccg aacggttaaa cgggcatttt tcatactaaa
     1681 caaaagacgc tccgtctgct ggtaaaaaag gcggtcaaac gctccatnnn nnnnnnnctc
     1741 tgcaagcgtc agtttgccgc agatctgcac ccagtttccg tacgcacagt ccgctggctc
     1801 ttccagaatt gcccgaacct taagatgttc ggaaaccttc tccataaaat cagcctgtat
     1861 atgatctgca tatacatacc agaaatctgc tcctgcctcg atctgacaga ctcttcccgt
     1921 gcaggaaacc tgttttccgt caaacgactg cggataagga aactgctccc gaatccctgc
     1981 aaaatgacag agcaagatcc ataagatcag aatacctgtc aaaaacaaca acggtctctt
     2041 tttgaaatat atcaagtgcc gctctctgtc tgtgcttcct ttgtcagatt cggatcaacg
     2101 ccaaccgatg gaaccgcacc gttatcgctg ctcccggtat cgttttctcc gtctttctcc
     2161 cgtatgaaac tgctgttaca ggaaaacacc tgtgacagat ccagtgtatc acgcagaagc
     2221 acatccgagc tgccttccac ctggatctgg actttcgtga tatcctcaca gctttcgcag
     2281 atggaattta cgatcgcata aaccgtcact tccggtgtaa ccgaagatcc tgccactgcc
     2341 tgattaaatt cactgcttaa attcaccgta cataaatccc cctccgtacg gatttcacgc
     2401 acaaccgtat cagacggcac aactgcagta agtttgctgc ccgccggtcc tgcgatcagc
     2461 tgctcaacca ccagtttttc aagagaatca tccgatgcat aatgcacgtt tcgcatctgt
     2521 tttgtcagtg aagtgccgtt tctgctaccg aaataaagcg gcaatgcctc atattgatat
     2581 gcattgatac tgtcaccctc cgaattgata aacgaagagg catgcatcgg ccctactgcc
     2641 tgattttccg agttcagaag tgcctcacct gcaacggtaa ataccacttc ccggacatcc
     2701 gtgatctgcg tcatcgtctg caccactgcg gcacgcagca aaacttctct tgtatcatcc
     2761 atctgcttat aagcatcatt aaaatcaatc gtcagccgtc cgtcctttaa agtcgatacc
     2821 gcagcagtca gtccctccgg aaaaacactc tgatagtcgt ttcgattaaa cgtcccttcc
     2881 attgcagaaa gcaattcttc caccagtttt tccccgcctt ctgcttccgg ctcatattgc
     2941 acttcccgaa gtgcagtagc ctctgcatta cggcaaaaaa ccttataacc gctctgttct
     3001 gcattctgtg tgccctggca actcgtgagc atcaggccaa gaaacacagc tgctgcaaat
     3061 cctgccattt ttttcatatt tttgttctcc attccggcaa aagcccctgc caggtgaacc
     3121 gttgtatcag ttcacataaa tcagcgggat ccgaacagta aaggtcgtac cttcgccctc
     3181 tttactgtaa accttgatcg ctccgcggtg catcaggatc gcacttctcg tgattgcaag
     3241 ccccagaccg gttccgccga tctctcttga gtgtgattta tccacacggt aaaaacgttc
     3301 aaaaatagca tcctgtgact cttttggaat accaatgccg gaatcttcta cttttacata
     3361 aaaatatttg ttgtctacat taagcgaaac atgcacccat ccgccctctt tgttgtattt
     3421 gatcgcattt tcgaccagat tggacagggc aagcgtcagc ttcgtctcat ccacttcggc
     3481 attgaccggg cggaaactct ccaacacaag gttgatatcc tgcttgtccg cgatcggtgt
     3541 cagtctcttg atgatcagct ccagcagttc attgatattt ttattttcaa tatgcagatc
     3601 tgctgccttt ttatccatct tcacaagtga aagaagatcc gtgatgatct tattctcacg
     3661 atcgatctct tctgcgatat ccgccataaa ctcctgataa agctccaccg gtgcatccgg
     3721 ctgtgccaga agagaatctg caagtacttt catcgatgtg atcggtgttt tcagctcgtg
     3781 tgacacgttg gagacaaact cctgcctaga atcatcgaga gctttcatgc gggcaagcat
     3841 ctgattgtag gcctctgcaa gctgctgtgt ctcagtgtag tccggtatgg agatatcagc
     3901 atccagaaag ccctcctgca cctggctcaa agatttggta accttttcaa acggctttgc
     3961 cagcatcttg gaaagatagt atgcaatcag aaaaatggca atccccattc caatctgaag
     4021 gatcagtact ttccggttca gatcatcacg gttcttacga atatctctgg tcggtgtact
     4081 gacgatcatc acgccttcca cttctttgct ctgagcatca tagatcggta tggtaatttc
     4141 aataaagccg tttttcctgc tgtagctctg tccatcctca ccgcgaaaac agcggatcac
     4201 ctcttccgcc acaatatatt tgccctcatc tgtctgatcg gtatccttaa taatgcgaaa
     4261 ctctttattg acgacaagaa tcctgccgtt gtaaagattc gccatctgct gcagctccac
     4321 atctgtgatc ttggacttct caccgctcag gtaatcggtc ttggaaatct gggctgtgat
     4381 cagatcgctc tggctctgta taatactgat acgctgtttc accgcctgat cctgataatt
     4441 cgttaaaatc ccgtgtttga tcagcatgat cggcacaatt ccaacgatga taaagatcag
     4501 gcacatgcga aactgaagac tgtttatgta ctttcgtaat ttttcgtttt ttttcttttt
     4561 tctgaacact tgctcccctg tgcctgctcc cttccttcag tgtacctttt caagtcgaat
     4621 gcacgtgaga cttggcatgc gattctggtc agttttgctg acatttacct gacagttatg
     4681 aatgctggaa ataatatccg acgccccatt tggtgtgtac atactgcggg tcgctcggat
     4741 ttatctcaat cttttctctc agtctccgga tatgtacatc tacggtacgc acatctcccg
     4801 gatattcata accccagaca atgttgagca gattctctct gctgtacacc ttgttcggat
     4861 gaaagatcag aagctccaac acatcaaatt ctttggcagt caggtttact tctctgcctg
     4921 caatgttgac gcgtctcgcc tc
//
LOCUS       DAWDWH010000159         4853 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_7048_length_4853_cov_14.521676, whole genome shotgun sequence.
ACCESSION   DAWDWH010000159 DAWDWH010000000
VERSION     DAWDWH010000159.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7048_length_4853_cov_14.521676"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1577..1586
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1409 a          946 c         1406 g         1082 t
ORIGIN      
        1 ggttgtgata cagagccaga tcgcagcaga agaaggtctt tttacgatca atgatgtcgt
       61 gcagagcatc agcgaaaaaa tgatccgccg tcatccgcat gttttcggag aggagagcta
      121 tgagctttta ggcggacatg ctgacagctg gaaggagatc aagcagattg agaaggcagg
      181 tatggcagaa gatcagaaaa aagctcagga aagagcagtt tatctggcaa cacagcagat
      241 catagaacat ttacagaaat cccttgacaa atatggggat aacgattata aataaacata
      301 gccaacaagg ctgttgtgtt ccggaagcga tgagcggacg ggatgccgca gccttgttct
      361 caggaatcaa aacagcaatt acaggaggaa aggtccatga ataagacaga attagtagca
      421 gctatcgctg aacagacaga attatccaaa aaagatgcag aaaaggcact gaaagctttc
      481 acagatatcg ttgcagatga actgaaaaaa ggtgaaaaag tacagctggt aggctttggt
      541 acttttgaag tatccgaaag agcagcaaga gaaggaagaa atcctcagag cggcgaaccg
      601 atgacgatcg cagcttccaa agctccgaaa ttcaaagcag gcaaagcact gaaagatatg
      661 atcaacaact aattcctgta tttggaatca tcagctgtcg cggaacattt ctgcggcagt
      721 ttttttctta tcagcaaaag gagaaatgac tatgagactg gataaatttt taaaggtttc
      781 cagactgatc aaaagacgta ctgttgcgaa tgaagcctgt gatgccggaa gagttctgat
      841 caacggaaga acggcaaaag catctgcggc agtcaagaca ggcgatgtga tcgagatcca
      901 gtttggaaca aaagccgtaa aagtagaagt cctggacgtg caggagacag tcaaaaagga
      961 caatgcggca gaactctata aatacctgta gcttccggat gtgagctttt ttacgctggc
     1021 ataaacaaaa gcggagcttc atatactttt gcaaagagtg tatggagaat ggcatggacg
     1081 aaaaacaggc tcagggaaca gcacacaggg taatgatcca gggaaggcac agcggcacca
     1141 tcagcggggt atcggatgtg ctttcttttg atttaaatga gatcgtactc cagacgacac
     1201 aggggatgct gatgatacgc ggacaggagc ttcatatgaa ccggctgaca ctggaaaaag
     1261 gcgaagtgga tatcgaggga aagattgaca gctttgttta ttccgaacag aaaggcgaga
     1321 acggaaaaga tacctccttt cttggacggc tgttccggta atgcacacgg tgatcgcagc
     1381 agaggccctc ttttttgtgc tggcctttgc aaaaggagca ggtatcatgc tgggctacga
     1441 tatcctgcgt gcgtttcgca gaggatttcc gcacgggggc cggcttgtcg cagcagaaga
     1501 ctttttttac tgggtgttcg cctggatttt tgtgtttttg atgttccgtg atcggaatta
     1561 tgggcagatc cgggggnnnn nnnnnngggg gtatgtgctg gcggcactga ttctggggat
     1621 gcttttttat attttgctgt tcagtcggag gatccttctc gtattttcga cttttttcgg
     1681 ctttctttcc cgcatgattg ccggtctttt ccggctgatt ttgatgcctg ccggaaaatc
     1741 ggtcaaaaaa ttgagaaaaa tggtcaggaa agcattgaaa aagaagataa aacaggttag
     1801 aatggtcata gtcaaaaagt aagtcagaaa gtcgggaagg gtatatgaaa aaaagaaaac
     1861 agacacgcag aaggagagat gcggcacaga gaagaggaaa gcttggcatc tctactgtga
     1921 ttgttgtgct gattgcagta tttttgctga gaggccatac aatggatcag agaaatgcgg
     1981 catatgtcga agagctggca cagctgcaga acaagatcga tgcagaagaa gcccgcacgc
     2041 aggacattgc tgatctggaa gcttatatgc agacagatga gtatgcggag caggttgcca
     2101 aggacaagct tggtctggtc tatgaagatg agattatttt taaaccagaa gagaactaaa
     2161 gaaaaaggag aaaggcagcg tgataagctt cctttctttt ttgtcgaaaa ctttttttgg
     2221 aggcgtaccg cctttgacaa aattttccgg aaccattcgc tatactgaat ccgttaaagc
     2281 agcgtggaaa aggctgtgtg aggaggcagt atgggggatt ttatcattat tacactgatg
     2341 gtcagcagct tctggctttt gtgggagatc atccgtgtgg tcagtatgga gtggctgaac
     2401 cgggatgaga ttacattgcc ggcgggcgag cttgggagaa gcgagctgct gcgttgtgca
     2461 gattctttcg aacagctttc cgggatgttt cagggcatgg ttaccaaaaa ggaggaactt
     2521 tccgggcagg atatggatga ggtgctcttt cgggtgcaga cgagcgtctg ccgcagctgt
     2581 tcggccgcag cacagtgctg ggactatgga agtgaaacgg ggatctgcag attcaaccgg
     2641 atcgcagaga gcattatcca ggggaaagag ctgaacaatg gaaaaaaaga ggcacttcag
     2701 aaacaatgtc tgcacggaga cttctttttc gaagcggtgg cagatcagat tgaaaaagcg
     2761 agaatggata tgctgtggca caatcgcctg atggagagca gaagtgcggc ggcggaccag
     2821 ctccacgaga cggcgtgcat catacgtaag gcggcggggc atatctgcga tgtcagacgc
     2881 gtagacggga gtatccgcca gcaggcagac atccgcctgc ggatgcacgg gatgatcctc
     2941 aaggatatct ggtgtatccg gcagccaaga aatatacagc tctatgtgac actccgcacg
     3001 gcaaagaaag gcagatgcat ccgcacaaaa gaagtgacag gacttctgtc tgcagcgttc
     3061 gggacgaggc tgatgccgga tgaacagggg aaaaccgtag taaattatga gtacagtacc
     3121 attttatttt atacggaacc acagtattac atgaccggcg gtgtggcgag gatcgccagg
     3181 gacggagaag tgatctccgg tgacaatttt tccctgtttc cagcggcaga cggtcagatg
     3241 atcctcagca tttcagacgg gatgggttcc ggtcttgcgg catacaaaga aagcgaggca
     3301 gtgatcgatc tgctggagca gtttcttggc agcggttttg acaaagagac ggcgattcgc
     3361 ctgatccaca gttcgatgct tttgcagaca gaagggcaga gttcggcgac ggtggatctg
     3421 tgcatggcag atctttacac cggagactgt gaattcttaa aaatcggtgc ttcaacgacc
     3481 tttctgaaac ggaagcactg ggtggagacg atcacgtcga ccagtatgcc gatggggatc
     3541 cttcaggatg tggattatga gtgcaccaga aagcggctcg aagagggcga ttatatcgtc
     3601 atggtgtccg atggcgtgat ggatgccctg ccgcagatgg atgcggagga gatgatcaaa
     3661 gattatcttc tacaaagtga aacggaaaat gcaaaagaac ttgccagatc cctgctgaac
     3721 tatgtattgc agtcggacag gcagcaggca agagatgata tgaccgttct ggtgggcggt
     3781 atgagaagaa gatagagaga acatatgaga caggtacaga attttatcag agaacagaag
     3841 atgatcatgc cgggagattt tgtcattgcc ggagtttcgg ggggagcaga ttctgtctgc
     3901 cttttctttg aactgcttgc atatcagaaa gagatcccgt tttccatgga agttgtgcac
     3961 atcgaacatg ggatccgtgg acaggacagc cttgcagatg cgaactatgt ggaaaacctc
     4021 tgcaaagaat atggtgtacc gtttcacctg gtttcctgtg atattaaaaa gatggcacag
     4081 gaaagcagcc tgacggtcga ggaggcaggg cggaaagccc gctatcaggc ctttgagcag
     4141 gcggcaaaaa agagcggcag gtcaaactgc aagattgccg ttgcccataa tctggatgac
     4201 caggcagaga cgatcctgat gaatctcgca aggggaagcg gtcttcgagg acttggcggg
     4261 atccgtccgg tgcgtgaaaa catcatccgc ccgcttttaa atactagaag aagcgagatc
     4321 gaggcggcac ttcgggggcg tcagatcaca tggaggacgg atgagacgaa ccgtcagact
     4381 gagtacacga gaaacaggat ccgcctggag atcctgccgc tgcttgcaca gggcgtgaac
     4441 ggacaggcag cgagccatat cgcacaggca gggctgcggc ttcaggaggc agaggattat
     4501 atccagagcc aggtgcagaa acttgcagag cgttacgtac attatgagga aaaagcggag
     4561 ccggaagtat ttcttgagcg agaaggattc tgccgtcagg agcatctgat gcaggaatat
     4621 ctgataaggt tctgcctgga aaaaatgatc gccggtcaga aagatgtcag ccgccggcat
     4681 atcggggcac ttctggagct tgtagctggt cagaatggaa aatcattaaa tcttccgggc
     4741 ggcatacgtg ccgtaaataa aaatgaattt cttgtttttg agaaaaacag atcaatacga
     4801 ataaagggaa atgaagctgc aaagtgaatg ggagaaaatt ttcagatcca ggg
//
LOCUS       DAWDWH010000160         4842 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_7066_length_4842_cov_12.022352, whole genome shotgun sequence.
ACCESSION   DAWDWH010000160 DAWDWH010000000
VERSION     DAWDWH010000160.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7066_length_4842_cov_12.022352"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1443..1452
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1227 a         1218 c          950 g         1437 t
ORIGIN      
        1 atacagtcag attaattaaa aacagcagag gtttttatct cctctgctgc tcttttttta
       61 aaaaattacg gtttaaaatt ctcgctcgcc catttcttcc agaaatgcca gatagccttt
      121 aaatgcttca tagatatctg ctttgcttgt cacttcgaac ggtgcgtgca tgttctgcac
      181 tgccacgccg cagtcaatga cttccattcc gtagtttgcc aggatataag cgatcgttcc
      241 gcctccgccc tcatcaactt ttccaagctc ggctgtctgg aaagccgcct cattttcatc
      301 aaggatcttg cggattcttg cgatgtattc tgcatttgca tcgttggaac cggattttcc
      361 tctggaaccg gtaaatttat tgaaggtcag tcctttgccg aaaaaggcac tgtttttctt
      421 ttccatgacg gccggataat ttggatcgta agctgcactg acatcggaag ataacattct
      481 ggaactctga agtgctctgc gtactttcag ttcgctgtaa tctccggctg catccataat
      541 ctctgccact gcattttcaa aaaatctgga atgcataccg gtcgcaccga cgctgccgat
      601 ctcttcttta tctaccagca ggcatacgca ggtgtactgc ggtttgtcaa gctcaaacat
      661 tgcccggtag gatgggtagg cacacactct gtcatcctgg ccatagccca tgatcatact
      721 ctcatccagt ccataatttc ttgcatttcc ggccggaaca gcttccagtt ctgcagaaat
      781 aaaatcggtt tcttctatat tatatttctc ttttaacagc tgaaggatct gccttttgac
      841 cggctctttt tcttcaccgg aaagcggtat actgccgact aagatattaa ggtcttcgcc
      901 ttctaccact tcatttccac gtttttccat ctgttttttg ccaagatgcg gaagcagatc
      961 ggaaatgccg ataaccggat cggatggatc ttcacccagt gcaatctcca cgcttgttcc
     1021 gtcttttttt gccacaacac cgtgcagtgc cagcggcatc gcaacccact gatatttttt
     1081 gattccgccg tagtagtgtg tttccagcat cgccagacca tcgctctcat aaagcggatt
     1141 ctgcttcagg tccagtctcg gagaatcaat gtgagctcct aaaatattca tgcccttttc
     1201 cataggttct tttccgatat ggaacagtgc cagtgttttt cccatgatgg aaacataaac
     1261 tttatcgccg gcctgcagtt tttcgccgga tctgcgaact tctttcagac tcctgtaacc
     1321 ttttgcttct gcttctttta aaatctcttt tacgcattct ctctctgtct tgcattttga
     1381 gagaaatgtt ctgtattctt cacagaaatc atatactttc tttctgtctt cttccgtata
     1441 agnnnnnnnn nnccagaaca cgcactgcct caatcgttgt gccgcccggt gagcatacca
     1501 tgtctttgag ttcaccggga tgtttacctg tctccagcac catctttgca ctgccaagga
     1561 ctgcctgtgc tgcgaaacga tatgcctgtg cacgcggcat accatccgca acggctgcat
     1621 ctgccatcgc ttcaataaac ataaatacat atgccggtga acttccgctg acggaaacaa
     1681 ccacatcgat cagacgctcc ggaagcattt ctgcttttcc acagctctca aggattttca
     1741 gcacatcctg catctctgcg tctgacactt cttcgttgca gcacacgccg gtcatgcctt
     1801 ccccgaccag ggccggtgta ttcggcatgc atcttaccag ctttactttt ttgccaaact
     1861 gctcttccag ccatgccagt gtttttcccg gtgcaatgga aatgataatt ttcttttcat
     1921 ccactgcatc tttaatctca tggatcactt cttcataaaa aatcggtttt actgccagca
     1981 ccagcacatc cgcacttttc gctgcttcta tattatctgc tgttacctga atttcataag
     2041 tatttctggc attttctctg ctcgcctcac tctttgccgt cgcgatcaca tcttcttttg
     2101 cacagatccc attttttaaa atgccgccaa gcatggcttt cgccatattt ccgcagccga
     2161 taaatccaag tttcatcgct tttcgcctcc tgtaaatcca gaacttctgt ttctgataat
     2221 gccaagttgt acaagttact ttgttagata aagtaattct cagctatcat gccagagaca
     2281 gatttttccg tttctgacat cacatatata ctttagcaaa tcaaagtacc atggtcaagc
     2341 ctcacctgtg ttcgacttaa agcagaactt gcttgatttt ctcatcttga gtcgctatac
     2401 taagaatgcc gggtttatcg atacccgtta cctaatttac aaaggagcaa ttttcatgca
     2461 aaaggacgct gcttatatga aactgatcga ggaaatctca ctgaacgcct ggccgtccca
     2521 caagattgag ctttatgacg gctggctgat ccggttttct cataattata cttaccgtac
     2581 caacagtgtg gaacaggttg gtgactcctt gattccgatt gaggagaaaa ttgcttactg
     2641 cgaggctcag taccgcaatt accacacacc gtctaatttc aagatcaatc cgcttctgga
     2701 ttcgtctttt gataagcttc tggaagaaaa aggctatgag atccgccata ctaccgaggt
     2761 tatgacgatg ccgatgacaa attttcatcc ttatccggca gaatccgtag aatatgaata
     2821 ttatggaagg aattcaaatc taccttcttc tgttttttat ccgggacata tcgctgtcca
     2881 gctccgcgac cggatcacgg acgagtggat tgagtcgctg tttcgtctga acggcacgac
     2941 cagaccgacg cttcgccgca ttgtaccatc gatgttcaaa gcgatcccga aagagacgat
     3001 cgtcgcctgt attgaaatcg aaggccgcat ggttgcctca ggtcttggta ttctcgaccg
     3061 cggacacatc ggactgtatg cgatctatgt cgatgcaagc tgccgcagaa agcatttcgc
     3121 acgtgcgatc tgcagttcga tcctgaccga ggctcagaaa aaaggtgcga cacacgctta
     3181 tcttcaggtt gtaaaaggaa atactgccgc taaaaatctg tatacatcac tgggatttga
     3241 ggatttttat acttactggt tccgttccaa agaagtctga agaagaatca aaaaataaac
     3301 aaacacacga aaggacatta ttatgtggat tgctgaaaac tggaaagatt acgaagtgct
     3361 tgacacttcc aaaggagaaa aactggaacg ctggggagat tatactctgg ttcgtccgga
     3421 cccgcaggtt atctgggata ctccgaaaac actgagaggc tggaaacgcc cgaatgcaca
     3481 ctatcatcgc agcaaacgcg gcggcggtga gtgggaattt tttgatctcc cggaacagtg
     3541 gagtattcac tatcagaaac tggggctgac ctttcagtta aaacctttca gtttcaaaca
     3601 taccggtctg ttcccggaac aggctactaa ctgggactgg ttctctgaaa agatcaaaaa
     3661 agccggacgc ccgatcaagg ttctgaacct ctttgcctac actggtggag ctaccctggc
     3721 cgctgccgct gccggagcaa gcgttacaca cgtagatgcc tccaaaggaa tggttacctg
     3781 ggcaaaagaa aatgcacgtt cttccggact tggagatgca ccgatccgct ggatcgtcga
     3841 tgactgtgta aaattcgtag agcgtgaaat ccgccgcggc aatcattacg acgccatcat
     3901 catggacccg ccttcttacg gtcgcggacc aaaaggagaa atctggaaaa tagaagaagc
     3961 gattcatccg ctcatcaagc tctgtgcaaa gcttttaagc gacaaaccgc ttttctttct
     4021 tgtaaattcc tataccaccg gactggctcc tgccgtcctc acctacatgc tggcaaccga
     4081 acttgctgct tttcacggtc atgtagactc ccaggaagtc ggacttcctg tttccggcaa
     4141 tggacttgtg ctgccttgtg gggcatccgg acgctgggaa atctgagaaa tctgatattc
     4201 aaggatatct ccggcatttt gctgcgaggt gcacactata tattgcgtaa tatatagtgt
     4261 gcacttctgc aatctgcaga agatccccca ccagtacaaa tttgctgctc accacttttc
     4321 atagaaatgg acgttttctt agactaaaat aaatcagtat tttctatttt tctttatctc
     4381 gtgcaacagc tcatctgaaa ccgcacaata cttttcatgc gttatcgtat cacagatgat
     4441 gctctttttc ccaataaaca caacatcttc aagattaaca gatgcattct catcaaaaaa
     4501 atcaaacagc tcctttttct ctttcagaaa ttctctgata taatagtccg ctttaaaata
     4561 aaccagactc tctttacaat tttctgtatg tatagattct gtaaattccg aatctatata
     4621 agaacccatt atctgtttgt atcgaatatc ttctctcaat tcatctacat gatccagata
     4681 tggacaaacg gtaaagcaaa caatctccgt gtgtcttaat atcacatcga tgaacgtttg
     4741 atattcgttt ttctttatta taattgcatt tctggcatta ttgcaacact ttacatactt
     4801 cattcttaat gtgaactact cggtaactga gttaccagag ca
//
LOCUS       DAWDWH010000161         4823 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_7091_length_4823_cov_15.294044, whole genome shotgun sequence.
ACCESSION   DAWDWH010000161 DAWDWH010000000
VERSION     DAWDWH010000161.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7091_length_4823_cov_15.294044"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1409 a          941 c         1260 g         1213 t
ORIGIN      
        1 ggctacctta acccaccgtc taaagccagt gggattgcgg tagccttatt tcaaatcttc
       61 ataaaaaagt aagaaagata aacaacatta atgcaaaatg taaaagtaat gtaaaagttg
      121 ccttcgaaat attgaaatgt actacaggat atagtagact tgtgggaaca aagcagtact
      181 ccagagtacc gtatgattct gccgcagcag ataaaaaagg acggtacttt tttggaaaac
      241 gaatcagggg agagaaaagg ggaaaggcaa tgatgaagga caaaaaagag gatttaaagg
      301 ggaaaatgtg gtattttctc ctgtacacag gtggatttct ggcggcagcc attctgatgc
      361 tgctgatttt tcgaaaggaa aacgcatcct ttaccaaaat ggcggacggt gttgcacagc
      421 attatgcagt gctgatgtgg attcgtaata cgctgcggca gtttttacat ggaaattttg
      481 cattgccgat ggttgatttt tcggttggac aggggtttga tgtgatcggg acgctgaact
      541 attacggcgt tggggatccc gtcaatttgc tgaccgtatt ttttgcggat aaccatctgg
      601 atcagatgta tatgtttctg attctgtttc ggatgtatct ttcaggcctt actttttctt
      661 attattgcag cacggccggg attcaaagaa aagcctctgt actgtgtggt tcctggctgt
      721 atgtattttg cagttttgca ctgatcggtg gaatgaagca tccgctgttt ttaaatggta
      781 tgctgtatct gccgctgctg cttgccggaa cggagaaagt cctgcaaaag aaaagcatcc
      841 gctttttaag cgtatcggtg gcacttgcct ttatgagcaa ctactatttc atgtacatga
      901 acaccatact gtgcggcatt tatctgtgtg tacggctttt cggacattat cgggaatacg
      961 ggatcagaaa gatcctactg ctgatcttaa aaatggcggc ggcgtggatc tggggcatat
     1021 gccttggagc agtgatcatt ctgccgtccg tctatgcctt tcttcacaat gcaagagtgg
     1081 acactgcggt ggaagaggca cagaactttt acagcattgc acactaccgg aaaatgatcc
     1141 tcggattttt ccagacactt ccgatgacga acggctggac agtacatgga acggcgatcg
     1201 gcggtcttgc cggtgtgctg atgctcttta cgtcaaaaaa aaggagtcgg gaaaattgcc
     1261 agctgaagat cggatttgtc gtgctgcttg tgttgctctg tattccgttt ggcggaaaaa
     1321 tgatgaatgg ttttgcttat gtgacaaacc gctggagtta cggcatggcg tttttatgtg
     1381 cactgatggc agctcaggag gttgcagatt taaaagaaca gaatacaaaa atattcctga
     1441 tccttggagc ggcagcgggc atccttgctg cagcattatc cgcatcgaac ggaaaagcca
     1501 tgcgatatgc aatcgcagct cttgcagtga ccgttctggc atttgcactt ggagcgattc
     1561 tggagcgaaa gcagagaaaa agactggcgg gatgtctggt gtctttcgtt gtgtttgccg
     1621 gtgtctgctg caatctcatc accttcttta caccggtcgg atattcttat gccgcacgtt
     1681 ttacaaaaag aggcgtgtcg gagtccgtat tactgaaccg tgccgtaaaa aatgtgcaga
     1741 atgcaaagct tgcggaagac ggattttatc gggtggaact gccgtcctcc ctgtataact
     1801 gctcgctggc agcaaatatc aacacaacag agttttatta cagtgtaatt ccgaaatcca
     1861 tgaaagatct ctatgtttca cttggaatgg caaaatatga acgtccgaat gtcatggaag
     1921 gtctggaaaa ccgtcagatc cttaaaaata tgctctgtgt ccgttatcag gctgataaaa
     1981 aaggaatcac ggtcaataag gatgcattgc cagtcggata cacgtatgac aagatcatga
     2041 gcagagaaga ttatgaccgc ctgacgccgc ttgagtgcca ggcagcactt ctggaatatg
     2101 ctgttttaga tgacgatgct gaaaaaatcc tggaaaaaca ggggaagact tttgaaagag
     2161 gaaagagtcc ttctgatggt gctgttatag gtggaaatct gaaaattaca ggagaggatc
     2221 gggcatcctg gaaaaatgga acgttaaaag ggaaaaaaca aggcagaatg aaactgaagt
     2281 ttcagacaga agaaaagtca gagacttatc tggtgctgaa agacctctct tccagattaa
     2341 aagtaagaaa aaaacatatg ctttccgtgc aaagcaaaaa agcaaggcag gagattccga
     2401 tgtgtgcagt gagcaatgaa aaaaagatga aacgggatgt gatcgcagtc aatcttggca
     2461 tcaggcaggc gggaacctgc agcctgcatt ttcataaatc acatacttat aagctgaaag
     2521 agatggaaat ttatggtatt tccgaaagtt ttatcaaaga acagacaaaa aagcggcgaa
     2581 aagaatctat gacagatata aaacagagta caaattgtat caagggcagg atcagcgtat
     2641 ccgaagataa gattttgcag ctggcggttc catacagcag gggatggcac atttttgttg
     2701 atggaaaaaa ggaaaaatct ttcgccagta gtgttgctta taccgggatc tttcttgaaa
     2761 aaggggagca tacggtcgaa atgcattata tcagcccgtg gatcattccc ggtacggtgc
     2821 tgagtgtggc ggcatggatc tggatgccac tgtcttttgc cgaaaaaaag agaagaattt
     2881 cggtagttaa gagaataaaa tagttcagat ttttgaaaaa gtggctattt gtcgtaagga
     2941 aaatgacgga tagccgcttt ttttgttgtt attaaggttg aaatctgtac caaaatgcat
     3001 tatcatatag aattaaagcg gtaaagtttc aagagaaaaa caagaacaat actttaccag
     3061 gatgtatgca gagatatgca ggcatatgca aaactgcaga tgagaacgga ggaaaaggat
     3121 atgagttaca acatcgcagt cattccaggc gacggtatcg gaccggaaat cgtgcgtgaa
     3181 gcacagaaag tgctggatgc ggcaggaaaa aaatatggtt ttgaattaaa atatacagaa
     3241 gttttaatgg gaggagcatc catcgatgtt catggtgtgc ctctgacaga tgaggcgatc
     3301 gcaaccgcaa aagcaagcga tgccgttctg atgggctcca tcggcggaga tgcaaaaacc
     3361 tccccgtggt acaaactggc accgaacctt cgtccggagg caggactctt aaaaatccgc
     3421 aaagagctga atctgtttgc aaacttaaga ccggcgtacc tgtacgaaga attaaaagag
     3481 gcctgcccgc tgcgtgatga tatcatcggt gacggttttg atatgatgat catgcgtgag
     3541 ctgaccggcg gtctgtattt cggagagcgt cacacaaccg aggagaacgg agtacgcaaa
     3601 gccgttgata cactttctta tgatgagaat gagatccgcc gtatcgctgt ccgtggattt
     3661 gacattgcaa gaaaacgccg ccataaagta accagtgtgg ataaggcaaa cgttctggac
     3721 tcttccagac tgtggagatc cgttgtggaa gacgttgcaa aagattaccc ggatgtcaca
     3781 ctggagcata tgctggtaga caactgtgcc atgcagctgg tgcgtgaccc gaaacagttc
     3841 gatgtcatcc tgaccgagaa tatgttcgga gatatccttt ccgatgaggc aagtatggtg
     3901 accggttcca tcggaatgct gtcttccgcc agcctgaatg aaacaaaatt cggtctgtat
     3961 gagccaagcc acggttctgc accggatatc gcaggcaaag atctggcaaa tccgatcgct
     4021 acgatccttt ccgctgccat gatgctgcgt ttctcactgg atctggatga agcggcagat
     4081 gcgatcgaag cagcggtaaa acaggtactc aaagacggat accgcacagg cgatatcatg
     4141 tcggaaggat gcacaaaagt cggaaccgtt cagatgggag atctgatcgc agaacgcgta
     4201 taaaaccgaa tccggcatca tataaatgga ggtatgttac aatgaaaagt gataatgtaa
     4261 aaaaagggat ccagcaggca ccgcaccgtt ctctgttcaa tgctcttggc atgacaaagg
     4321 aagagatgga gggtccgtta gttggtatcg taagttctta taatgaaatc gttccgggtc
     4381 atatgaatct ggataaaatc gtcaatgcag taaaacaggg tgttgccatg gcaggcggta
     4441 caccggtagt attcccggcg atcgcagtct gcgacggtat tgccatgggt catatcggta
     4501 tgaaatattc tctggtaaca agagatctga tcgcagattc taccgaggca atggcactgg
     4561 cacatcagtt cgatgccctg gtcatggttc cgaactgtga caagaatgta ccgggacttc
     4621 tgatggcagc cgcacgtatc aatgtgccga ccgtatttgt aagcggcggc ccgatgctgg
     4681 caggtcatgt gaagggacgc aaacgcagcc tttccagtat gtttgaagca gtcggagaat
     4741 actctgcagg caaactgacc gcagacgatg tatgtgaatt cgaagaaaaa gtatgtccga
     4801 cctgtggttc ctgctccggt atg
//
LOCUS       DAWDWH010000162         4822 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_7093_length_4822_cov_9.866583, whole genome shotgun sequence.
ACCESSION   DAWDWH010000162 DAWDWH010000000
VERSION     DAWDWH010000162.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7093_length_4822_cov_9.866583"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1072..1081
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1437..1466
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1140 a         1249 c          711 g         1682 t
ORIGIN      
        1 agtatcaaat ccaagactgg agatcaggca aaagatcaga atcccgtaca atccatattt
       61 caaaagtgag tctacaaact gttcaactcc tttatctgct tccgaacgtt caaatgaacg
      121 cctcacaatc ttcctgatcc attttatcac cagtcttccg ataaagaaag caaccagtgc
      181 cgccagcact tccagtccaa atgtaatcag tcccggaata ctgtcctgca gatattgggt
      241 aatctgattt acctgtttta ccgcctgctg ggtagcttcg gtcacttcct gagtcgtatc
      301 tgcgatcgtt gttgtatctg tcatatcgtt tgctcctctt atttcagtgc cagaaatgca
      361 cttagatttc ctcttttatt tcccgttgtg cgatgtgaaa acagaatatc cgggttacag
      421 tgtgtacaca catttgtcac agcaatatgc ttcttcaaaa ttccggcttc ctcgaatacc
      481 aattcatttg ctttccacag gttcaactga tatttaccgt tttctttctg atagaataat
      541 tctggccaat atttttcttc aaagcttttc tgaaatttta caattacatc ttcacttacc
      601 tcgtaacagt cctgacatat agacggacca atagccgcaa gtatgtcttt aggatccgaa
      661 ccatatgttt tttgcatcag ttccactgtt acttttccga tctttccaac cgttcctctc
      721 cagcccgaat gcgacagtcc gatcgctttt ttcacaggat ctacaaaata cagtggtacg
      781 caatccgcat aaaatgtcac cagacagatt cccggtacat ttgtgatcat tccgtccgtt
      841 tccataaaac gtcttccctt gtcctctgct cttactaccg ccacatttgt tgtatgtgtc
      901 tggttggtaa acgtcatatc ttccggcttt accccaatgg ctcttgcaat ccttctctgg
      961 ttttcctcaa catcttccgg atcatcgcct cttgttgtgc tgatgttcat agttgcccag
     1021 catcccttgc tgacacctcc gagttttgta gaaaatccat gatgcacgat tnnnnnnnnn
     1081 nccgtttctt ctaacattgg ataactcaga aacggaaccc catctactgt tttttcatca
     1141 aatatatgtt cctgattttt atactgtaat ccaagactca ttctcttctc ctatatttcc
     1201 acataatcaa atgcattctg atataaaata atcctgtcat cttctatact tactacatca
     1261 aacctgcacg ggatttcatc tattccattc accgtcatat aataaagtgc actttttgag
     1321 ataactctct gtttggcata atctaccgcc tctgccggag ttcctttccg gtcatctgcc
     1381 cggtacttca cctcgcaaaa caccagataa tcgccatctc tggctattat atcgatnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnctcc gagtctgcac cgataattat attccagaat
     1501 ttcatatcct ttttctgtca gatatactcc tgccattctt tcataataac ttccggtttc
     1561 acggttattt cgtctttgtc ctcttttcga ccctgccatt ctttcattcc tttacactgc
     1621 tgattccatg cctagatacc gattttccgc agtgtctgca tctttatata tatttcttta
     1681 taaaagtcct tctgtgtatc ggagatggtc ccagttcttt cagcccctca atatgcttct
     1741 tcgttccata ccctttattg cttgcgaaat cataccccgg aagtacttta tcatactcca
     1801 ccatcagccg gtctcttgtt acttttgcaa tgatacttgc cgccgcaata gacacgctct
     1861 tggcatcacc tttaatgatc ggcacctgtg ggatctctac ctccggtatt gttactgcat
     1921 cattcaacaa aagatccggc tgcaccttta gattgccgat tgccatacgc atcgcctcat
     1981 aagtagcctg caaaatattg atctcatcaa ttcttgccgg acttacaaca ccgattcccg
     2041 ttgcaacagc acgttccata atctcatcgt acaatgcctc tctttttttc tctgaaagtt
     2101 tctttgaatc attcagatac aaaatcgggt catcctttgg caagataacc gcacctgcca
     2161 ccacaggacc tgcaaatggc ccacggccta cttcatcaat accgcaaata tactggcagt
     2221 cgctgtgttc tttttcataa acactcattg cttctgttct ttccagttct tttttcagtt
     2281 tttcctgttg cctttttagt ttttcttctt cccgtttatt catgacggat cactcctata
     2341 ttgtcaaatg gataaattct tacccatgcc ctgccaatca gatcttcttt tgtcagtacg
     2401 cctacacttg gatccctgct gtccatacta tggttacgat tatctcccat aacaaaatac
     2461 tcattttctt ttaatgtaat cggatctgcg gcagtctgtg gatctttgat caattcattt
     2521 ccatagatat cgctcaaaag cctctctccg tcaatgtata catatccgtt ttttatctgc
     2581 acagtctctc ccggcattcc gatgatacgt ttgatataat atgtattttc ttcgtatttg
     2641 tacggaaata caataatgtc ataacgtttc ggatcacgga agcggtaact gatcttatcc
     2701 acgatcagat tatccccgtt ttgcaacgtt gtctgcccta caaatgttat gataaaatac
     2761 gttaacccta tgatcagcag aatatagaca atccatcctc caagctctct caaaatgctt
     2821 tcttctgcat cttctcttga acgtctagcc atgttctttt gttgtttccc ttctatactt
     2881 tctgcctctg tttttaatat gcatccgggt actccagtgt cagtttgccc aaacgtccgt
     2941 tgcggaagtc atccatcaga agaaccgaag ctttttctgt atccagttca tttccacgaa
     3001 ccagacaatg tctgctctct gcaatttttt ccagcattcc gtatggatcc gtgtcttctt
     3061 ctacattata tttactctca agtactcccg gataattttc attcataaac tttacaagtt
     3121 ctgccgcaag ttcttctgtc tgaaggatct catctttgat cgatccgata aatgccagtt
     3181 tcaggcctac tgcctgatct tcaaatcttg gccacaggat tcccggtgta tccagaagct
     3241 ccacattttt attcagtctg atccactgct ttccttttgt cactcccggt ttgttaccgg
     3301 tttttgcaca tgcctttccc gcaagtgcat tgataaatgt tgattttcct acattcggaa
     3361 ttcccactac catagctctg accggacgat tcaaaatccc acgctttctg tctctttcaa
     3421 tcttttcttt acatgcttcc tggatcacac cattgataga tttgatcccg ccgcctttct
     3481 tggaatttac cttcacgaca gagaatcctt tttcacgaaa atattctgac caggcatcat
     3541 tccacttatc ttctgccaga tctgacttat ttaaaaggat cagtcttgct ttattctttc
     3601 ccagttcatc aatatccgga tttctgctgc tgatcggaac tctggcatca accagttcaa
     3661 taaccagatc tattaatttg atattttcct gcatcatgcg ttttgctttc gtcatgtgcc
     3721 caggatacca ttgaaaatgc atatattatt tctccttatc ttctatttca caaatcccca
     3781 gtcttttttc ggggatgcca caaaccatgc ctttccatat atataagaac gttttacttt
     3841 tcctatatcg gcactacggc tgtcctcact gttttttctg ttatctccca gaacaaaata
     3901 ttcatcaccg ccaagtttca acttctcacc ggcaattcca gctgtatcta tttttgttgt
     3961 tttataatct tcttccagtt ttttaccgtt aatatagacc tgattctcca caatctgtac
     4021 cgtctcgccc ggaagtccga caatcctttt ggtataataa tgcgaattct cattgccttt
     4081 cggtttgaac acgataacat ctcccctttt cggtgaagaa gcattgtaca caatccggtt
     4141 taccagtact acatctgcat tcttaagcac cggactcatg gaatctccta ccgtactgac
     4201 ccgttgtccc cagtaccaca cagcaacaaa tgccaccaga caggtgacca cgatcttaaa
     4261 aatccaggta aatgtagaag cgatcatttc aagattaaga tgtatctttt tctttgcttt
     4321 tggaagattt ccgaagcctt ttttctttgc tttgcgtctg cgataaccgg ttttccggga
     4381 tcttcttctt ttttcaaaat ccagattact tctgcgtctc ataaaatccc cttatactga
     4441 aaacttgcca ctggcaactt ttcttacata ctttgatata tcagaaaaag gacaattaca
     4501 ttatgtagtt gtcctttttc tcattttgtt acattttcga ttattttact aattctttaa
     4561 ctttagcttt cttaccaacg cggtctctta agtagttcag ttttgctctt cttactttac
     4621 ctcttcttac tacttcgatc ttctcaacgt ttggtgagtg taccggccaa gtcttctcaa
     4681 caccgattcc gttagaaaac tttctaactg taaatgtctc tcttgctccg ccaccctgtc
     4741 tcttcaggac tgttccttcg aaaatctgta ctctttcacg gtttccttct ttgatctttc
     4801 cgtaaacttt aacagtatct cc
//
LOCUS       DAWDWH010000163         4804 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_7119_length_4804_cov_9.069488, whole genome shotgun sequence.
ACCESSION   DAWDWH010000163 DAWDWH010000000
VERSION     DAWDWH010000163.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7119_length_4804_cov_9.069488"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    830..839
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1681..1690
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1255 a         1040 c         1018 g         1471 t
ORIGIN      
        1 acatcttgtt atggaattat ccggaacgat tcacgaatac atccgaaaca atcataatga
       61 ttctcaggta cttccggcac ctcttgccgt acgactcaac gctgatgata aaacttatgt
      121 cgaacctgat atctctgttc tgtgtgactc gaagaaaata tccgaaagag gtattgaagg
      181 tgcaccggat tttattattg aaattgtgtc tcctgctaca caaaaacgtg actatggcat
      241 caagcttttt aaatatcgta atgccggtgt tcgcgaatac tggatcgtga acccatcaaa
      301 agagattgta aatgttttct gcttcgaaaa tgaagaggaa aataacaacc tttactcttt
      361 tgaagaggat attcctgtag ggatttatcc agacttctca atcaatattc atgaattgct
      421 gaaatagttc cggtaattct atcacattag caagtaattc atctgtaatg taagagtccg
      481 atgttgcagg gctgccgctt tcacgacagc ccttttctgt ttttcatatt aattgctttc
      541 tatgcatcca ccagaagaaa caggacagct gactggttta tcaaaactcg gattgtatgc
      601 ataaaaattc ttagaatcca gttcatcctg taaaatgcga agtgcttcac cgaatcgctg
      661 aaaatgcacg atttctctcg cccttaaaaa acggatcgga tctgcgacat ccggatcttt
      721 taccattctc agaatattat cgtaagtgct gcgtgctttt tgctcagcag ccatatcctc
      781 atacaaatcg gtgatcgcat ccccttttga ctgaaattca catgcattan nnnnnnnnnt
      841 aaatggaata ccaccggcag cctgcggcca gattcctacg gtatgatcca cataatacgg
      901 tgccagaccg gatttttcaa tttcttctac tgaaagacca cgcgtaagct gatgaacgat
      961 tgttgatact atttccagat gtgccagttc ttccgtgccg atgtcattta aaacacccat
     1021 aatttttcga ttcggcatag aaaaacgctg ggacagataa cgcagcgatg caccaatctc
     1081 gccatccggt ccgccgtact gactcatgat gatctgggcg attcttccat ccgggttttt
     1141 gatattaacc ggatattgca gtcttttttc ataattccac atatcagcag ccgcctcctt
     1201 cccatggcca aggtgtttct acccataaaa aactctcgct cgcttcatcg tctgcggtat
     1261 caatggtaag cggaccataa tactttgcat attctttcag tgcggcaact cgacggtttt
     1321 tgtgttccat gaaataagaa aaagctgctt cgtcacacgg atgtgtgtcc agataaagga
     1381 gcatatcgtt gactgcaaaa ctgcattcat tgatttcatt cagcaaactc tttttgtccg
     1441 gctgttgtct tcttttgcat tccatatttt tgtctgtctg catttccatt cgaggtcgat
     1501 tgttcatcgg ttgtctgtta tttgcatttc gcatacagga attattggtt ctgtaatgat
     1561 tgcagccatt catcgacatc tgcctccttt tccgcaaaat gttttttcaa ggcatggaaa
     1621 tatggtgcct acttttaaag catgacataa agaaaacgat ttatcaaatt tctgccacgg
     1681 nnnnnnnnnn ttgatactgc aactgtgttt cctcctatta aataatcagt tcaatataag
     1741 tttatgaaaa atgatgcaaa tagttccttg cagtttttac ctgttccgtt cggggtgttt
     1801 caaacgctga aaagcagtgc aggatgggta tatttgaaaa aacggagagg gtataatgaa
     1861 aatacagata gaaatactta aaagagtgtg atagtataga tgcgttaccg cctccaatac
     1921 tggtaacagg aaggaggtga atcatttgga ttttcttact tctcttattg tcactgttgt
     1981 gggtggtgta atttgccatt gcatcatcaa atggttggat agtgataaat agtcggtaac
     2041 cagcctatgg atttgttctg ccattaaaaa gagaatagaa aaccccagag ttgccgctct
     2101 ggggttttcg tttgtagtcg aatcatttgg actacctact tctctttgcc taatggcatt
     2161 atagcatatg cattcttcat tttcaatatg ccttttgatt ataggttatt gcagtacggc
     2221 ggaccagata gcgaaatcgg tgcatcgcca cgttatctat tgctgtcacg gcagcccctt
     2281 aatatcagat tactatatgc taagtgaaat tttttcctga aatggtgctc gatatgggtt
     2341 ccttttgact ttaaaatatg ttatgataca tagtaatcct aatagaaagg caggatttta
     2401 tatggcacag acaaacacaa aaaccgtcag gaggaaacgt aaggtaagac ttctgacaag
     2461 aaacggtcag tattatgatt acaatcttct ggcctgtgta gtccttctca cctgttttgg
     2521 gcttatcatg ctttacagta caagtgctta tgatgcccag gttgagtatg gaagtgatac
     2581 atattttctt ttccggcagg cattgttcag cgttggtgga ttggcagcga tttttgtttt
     2641 ttccatgatc gattaccatt tttacgccag atatgccggc tatttttacc ttttcagcaa
     2701 tcttttgctt ttggttacca aattcatcgg tcacgaggta aacggtgcaa aacgatggat
     2761 caaaattggt ccgatccagt ttcagtcagc agaacctgcc aaactggcaa tcattctgtt
     2821 cctttctgta ctgatcgtgc agttcggcaa aaagttaaaa ggctttaagg caccggctgt
     2881 tctttttgct tttggtgcac ttacgttcct tctgacctgg aaatttaccg aaaacttaag
     2941 taccgccatc atcatcctcg gcattacctg tgcactgatt tttgttgcac atccaaagac
     3001 tgcaccgttt gttctcacag ccggcattgg tcttcctgtc ggtatcgtag gcgtgcgtgg
     3061 atatgtttct tcgcttggtg caagtggaaa cttccgtttc aaccgtgtcc ttgcatggat
     3121 cgatccggaa aaatattccg atggcggcgg ctatcaggta cttcagggat tatacgctat
     3181 cggctccggc ggtctgttcg gcaagggtct tggcaacagt actcagaaaa tccagtctat
     3241 cccggaagct cagaatgata tgatcttttc gatcatctgc gaagaacttg gactctttgg
     3301 tgctgttctt ctgaccatcc tgtttgtttt tctgctgtac cgactgatgt ttatcgcaca
     3361 gaatgcaccg gatctactcg gttctctgat cgttaccggt atttttgcac atatcgcttt
     3421 gcaggtcatt ctgaatattg ccgttgtatt gaacgtaatt cctacaaccg gtgttacgct
     3481 cccatttatc agttacggcg gtacttcgat tctcttcctg atgacggaaa tgggaatcgc
     3541 cctgaatgtc tcgcggcaga tccggataaa agacaactga tgattatttc tgcaaaggga
     3601 ggttcttaat tatgatcgat cttcatgttc attctacctt ttcagacgga acactgacac
     3661 caacgcagct ggtagaacat gccatggaaa atcatcttac ggctttcgct ctgaccgatc
     3721 acgacaccgt agatggtctc ggtgaggcgt ttcaggctgc cgaggacaaa ccaataaaag
     3781 tcatttccgg cattgagttt tctactgaat acaatggtca ggatatccat atcgtcggtc
     3841 tcgattttga ctggcacgat acttctttct gccaggaact ggatcatttc cgcaatgccc
     3901 gttttgaacg gaatcgtaaa atggctgaaa agcttgccga ctgtggattt ccgatcacta
     3961 tcgaggcttt gcaggaaatg tttggtgatg ccgttctgac aagagcacat ttcgcacgtt
     4021 ttttatatga aaagggctgt attccatcta tgaatgatgc tttttttcat tatattggaa
     4081 atgacggacc ttgttttgtc ccacgtgaaa aggtaacccc ggcaatggcc gtgcacctga
     4141 tccataaact ggatggaatt gccgtccttg cacatccaat gcagtaccac ctttctgatt
     4201 ctcagttaaa ggaactgatc cgtaccctga agccagaagg cctcaacggg atcgaagcca
     4261 tgtattcacg tcactcggtt tctcaggaaa accgtgtacg ccggctcgcc caggttatgg
     4321 gacttgccgt ttccggtggt tctgattttc acggcagcaa tataccgggg attgatctgg
     4381 gtgtgggacg cggcaatctt cgcataccgg acacgacttt tgagaacctc agaaaacatc
     4441 gtgacagaca atactaaaag gagtttttat gatgaaattc agagaacgtt ttatgatggg
     4501 agaagtccca tttgatgata tcttttcttt aacagacaaa tggaacttca gtgatgaagc
     4561 atgtactttg cgtgaatatc tcggccttac tgccaaagaa gaagatgtgt ggatcagcga
     4621 cagtgacgat gccctggaag aattaatgga aaaagaaaaa aataccaagg cagttttcct
     4681 ggatctggac ggaacgctct ttaccgacga aaagactttt tctgccggca actgtcaggc
     4741 gatcgaagag gctcttgcag ccggacacca tatcgtgatc tccaccggcc gccctcttgc
     4801 cagt
//
LOCUS       DAWDWH010000164         4725 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_7232_length_4725_cov_8.695075, whole genome shotgun sequence.
ACCESSION   DAWDWH010000164 DAWDWH010000000
VERSION     DAWDWH010000164.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7232_length_4725_cov_8.695075"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3869..3878
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4379..4491
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1384 a          897 c         1296 g         1025 t
ORIGIN      
        1 agaaaatgaa agctggaatc agagaggcag aatccattct gaaagagcag atggaagaat
       61 tttgcaactg gtatcgatgc cttgatgtga taccgcgtat tggaaagatc aaagaagaag
      121 ttgcgacaga cctagatctg cgtctgtata aaatcctgca tcagcttcca atggaagtat
      181 ttgaaaaaga aacactggaa aaacagatcc atcgagcggc acagaaagtg accaacaaaa
      241 tgctttttga actgcgggga cggatcagcg atgcagcatt tcgggagtgc atagatggac
      301 tggaagagat ttatgcgtgc caggtttcaa atgcgtttga ctttaatgaa aaaggagaat
      361 aaaaatatga cagagcataa cattgtcata ggaagcagag gaagccgtct ggcggtgatc
      421 caaagtgaga tggtccggga ttttatcaga gagaagaaac cgggatgtca ggtagaaatt
      481 ctgaccatga aaacgaccgg tgacaaaatc ctggatcgca cgctggagaa aatcggcggc
      541 aaaggtctgt ttgtgaagga actggataaa gcacttcggg atggatgcag cgatctttcc
      601 gtacacagtc tgaaagatat gccgatggaa ctttccgaag agctgccgct gcttgcattt
      661 tccaaaagag aggatccaag agatgtgttg gtgcttccgg aaggagcaga aaaatgggac
      721 agaacgcttc cggtcggatg ttccagccag agacggatgc tgcagcttaa agagctgtat
      781 ccggatgtaa cctttcttcc ggtgagagga aatatccaga ccaggctgca gaaacttcag
      841 tcaggagcat acggagcact gatcctggca gcagcaggtt taaagcggct tggcctggaa
      901 aaccgcattt atcgttattt tgagccggat gagatcattc cggcagcagg gcaggggatt
      961 ctggcggttc agggaagaaa aggagaagat tatgcttttc tgaaagccta taacgatccg
     1021 gcggcacaga cagcacaggc agcagagcgg gcttttgtaa aatatttaaa tggcgggtgc
     1081 agttcaccga tcgctgccta tgcagaaatg aaaaacggaa agcttctgct acgtggcctg
     1141 tattaccagg aagcaaccgg aatatataaa aaaggtcaga tcgaaggaaa tccggaagat
     1201 gccgaaacaa tgggcatgtt gctcgcagaa gggctgaaaa aagaatgtca tgcacaaagc
     1261 tgcactgctg taaaagaaga tgatataaaa ccgggaaaag tctggctggt aggagcaggt
     1321 ccttccgatc cggggttgtt tacactgaaa ggaaagcgtg tgcttcagaa ggcagaagtt
     1381 gttgtttatg atgcactggc aggtcaggct attttaagta tgatcccaga ggatgccgaa
     1441 aagatcaatg tcggcaagcg ggcaagccat catctgatgc cgcaggaaga gatcaacaaa
     1501 atccttgtga aaaaagcact ggaaggcaag agggttgtgc ggttaaaagg cggcgacccg
     1561 tttctgtttg gcagaggcgg ggaggaactg gagcttttaa aggcacatca gataccgtat
     1621 gaggttgtac cgggtgtgac ttctgcgatt gcagtccctg cctacaacgg gattccggtc
     1681 acgcaccggg atttctgctc ttcgctacat atcattaccg ggcataagaa aaaaggcgat
     1741 acctatgata ttgattttga ggcactggta aggacaaagg gaaccctggt ttttctgatg
     1801 ggagtgacgg cacttgcaga tatctgcgat ggacttttaa aggcgggtat gcgtgcagat
     1861 atgcctgctg ccattttaca gcagggaacc acggcaggac agaaacggat cagtgcaacc
     1921 gtttcaacac tgccggaaga ggtacaaaag caggggattc agacaccggc catcatcgtg
     1981 gttggcgaag tatgtgctct ggccgataga tttgcatggc atgaagcact gccgcttgca
     2041 ggacgtaaaa tcctggtcac aagactaaaa gaactgattt cccaaatggc ggaaaagctt
     2101 cgaagagaag gggcagaagt gctggaactt ccggcgatca aaacagaagc gatttcagaa
     2161 aatcaaaaac tgaagcaaag tatggaagct attcaaagtt atgactggat cgtatttacc
     2221 agcccttttt tgaaaaactg acagaacatg aagcggatat ccgcaaactt gcggcggcga
     2281 aatttgctgt tataggaagc ggttctgaaa aggaactgaa aaagcatggt attctggcag
     2341 atttaaaacc ggaaattttt gacggagaac atctgggatc cagccttgcc cgggcctgca
     2401 cggggggaga gcggattttg ataccgcggg cagagactgc aaatcaggaa ctgatactgg
     2461 aacttcagaa agtaccggat gtgcagatcg atgatgttcc aacctaccgc actctgtacg
     2521 aaagctcaga actgatcgat caaaaggcag aatttgaaca gggaaagatt gattttgcgg
     2581 tctttaccag tgcatcgacc gtgcgagggt tcgcacaggc agccaaaggg cttgattttt
     2641 cccttgtccg ggcagtatgc atcggaaaac agacgaagcg ggcagcagat gagcttggaa
     2701 tgcaaacttg gatgtctgaa aaagcaacga tggacagcgt tctggataaa gtaaaagagc
     2761 tgtgccggaa gcagttttaa atgtaggagg taacaaaaga tggatatgac aatccgacca
     2821 agaaggttaa gaggaaacga tacactcaga agaatggtga gagagacgag aatggataag
     2881 tcttccctga tctatccgat gtttgtaaaa gaagggaatg acatccggga ggagatcccg
     2941 tccatggacg gacagtaccg ttacagtgtg gactgcatgg gatttgaact ggagcggctg
     3001 ttaaatgccg gtgtgaaaaa tgtgatgttt tttggcatcc cggatcagaa agacgaagtg
     3061 ggaagcggag cctacaacga aaatggcatt gtgcagagag cacttcggga agcaaaagaa
     3121 aaattcccgg aactgtatct gatcacggac gtttgtatgt gcgagtacac atcacacgga
     3181 cactgcggca tgttgtgcgg acacgatgtg gacaatgata ggacgctgga tttgcttgca
     3241 aagaccgctc tttctcatgt ggaagccgga gcagatatgg tagcaccatc cgatatgatg
     3301 gacggccgtg tgcgtgcgat ccgtacgatg ctcgatcagg cgggacatac aggcacaccg
     3361 atcatgtctt atgccgtgaa atatgcatcc gccttttatg gtccattccg tgaagcggca
     3421 ggttctgctc cgtcttttgg tgatcgcaaa acatatcaga tggattttca taataaaaga
     3481 gaaggcatca aagaggctct gctcgatgtc gaagagggag cggatatcat tatggtaaaa
     3541 ccggcgatgg cttatcagga tgtgatcaca gaagtgcgaa aagtggtcaa cacaccggtt
     3601 gcagcctaca gcgtcagcgg agaatatgct atggtaaaag caggagccaa actaggctat
     3661 attgatgaag aacggatcat cggtgagatg gcagtcggtg cttaccgtgc cggagctgat
     3721 atttatctta cctattttgc aaaagagatt gcaaaaatga tggatgaagg gaagattgga
     3781 tgatgacaag atcagaagaa ctttttaaaa aagcagttac tttgattccg ggtggcgtga
     3841 acagcccggt gcgtgctttt ggaagtgtnn nnnnnnnnga ccgatgtcga tggaaacaaa
     3901 tatctggatt ttatcggttc ctggggaccg atgatcctcg gacacaacca tccggcgatc
     3961 aaagaagccg tgatcaaagc atgtgaaaaa ggactgagtt ttggagccgc cactgaaaaa
     4021 gaagtagaga tggcagagct gatgacagat gtgatcccgt cacttgaaat ggtgcgtatg
     4081 gtcaattccg gaacagaagc cgtgatgagt gccatccgtg cagccagagg ttatacaaga
     4141 agggataaga tcgtaaaatt taccggatgc tatcatggac attccgatgc acttctggta
     4201 aaagcaggtt ccggagttat gacagccgga atcccggaca gtgccggtgt accggcagga
     4261 tgtacacagg atacgttatc tgccatttac aatgatctgg caagcgtaga ggcactgttt
     4321 gacgaatatc caaaggaaat tgctgccgtg atcgtagagc cggtggcagc caatatggnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgttgacta
     4501 tgcttccgca aaaacctcag atactgctga gtatgcacgg tattttaaat atatgctgga
     4561 caatggaatc catcttgcac cggcacagtt tgaagccatg tttgtgtcag cggcacatac
     4621 ggaagaaaat ctggcttatg cactgcagag ggcggagaat ttcttaatgt gcaaatgaac
     4681 gaattccccg aagggcacgt aaaagtgcct tctttggaat tcgtt
//
LOCUS       DAWDWH010000165         4690 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_7298_length_4690_cov_9.219417, whole genome shotgun sequence.
ACCESSION   DAWDWH010000165 DAWDWH010000000
VERSION     DAWDWH010000165.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7298_length_4690_cov_9.219417"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2540..2549
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1134 a         1202 c          846 g         1498 t
ORIGIN      
        1 ccgaccgaag cggagcgtag aaaggattgt gggagtgcaa cgcacggaac aatcatgtct
       61 tagacttctg cccggtataa ataaaaaaat agaagaaaag ctttttcaag ctcctcttcc
      121 atcttattaa ctctcacatt tgctatgcaa acagctgctt tcccggtatt atgccagttt
      181 agattttgcc agctctgcca gtgttgcaaa tccttctgca tcattaactg ccatgtctgc
      241 caggatcttg cggttcattt caacacctgc cagtttcagt ccatacatga atttgctgta
      301 agacagacca ttgattcttg ctgctgcgtt gatacgagca atccacagct gtctgaactg
      361 acgctttttc tgttttcttc ctgcataagc acttgtcagt gctctcatta cagactgttt
      421 tgcaactcta tactgcttgg aacgagcacc tctgtaacct tttgccagtt tcagtactct
      481 gttatgtttc tttctagcgt tcatgccgcc tttaattctt gccatgtctt atttcctcct
      541 tcgcttattc ttataaataa ggtaaaatct tcttcatgtt cttgaaattt gtagcatctg
      601 tgatgattgc ttttctcaga tttctctttc tctttgtgga tttttttgtt aagatatggc
      661 tcttgtaagc tttatttctt ttcagttttc ctgcacctgt ctttttgaaa cgttttgcag
      721 ctgctctgct tgttttaatt tttggcattt tacgttttcc tccttgtata aattctgttt
      781 atcggctgta accttccggt tctggccgtt ctattttaac cctgcgttct taggaacgtt
      841 tttcggttaa aaacatcgcc atgctcctgc cttccatctt ggccggctta tctacgagtg
      901 cgatatcttc cagtgctttt gcgaactcat ccaggatatg tctgcttgac tgtacgtgag
      961 ccatctcacg tcctctgaat ctgagtgtta cttttacttt attacctttt tcaataaact
     1021 ttctggctga agcaatcttg gtattcagat cgttgctgtc gatattcggg gaaagacgaa
     1081 cttcttttac ttcaatcgtc ttctgttttt tctttgcttc tttttctttt cttgccagct
     1141 cgtaacggta ttttccataa tcaatgattt tgcataccgg tggtttcgcc tgcggcgcaa
     1201 tctttaccag atccagttcc gcctctcttg cgatcttcat agcttctctg gatgacataa
     1261 ttccaagctg ctgtccatct tcgccgatca gacgtatttc tctgtctctg atctgttcat
     1321 taatcattaa ctcgctaata gttttgcacc tccattaatg cgtgtaccac ctttgctccc
     1381 tcatagacca gaaaaaggtg gacagcataa ctgtccacct aataatcgtt ttccctgaaa
     1441 caaatctctc tgtttctgaa ctttattaac cgatagtcac cttttgtgct acggtgagag
     1501 tggacactct gctttgtttt ctcaataacc ttaaataggt taccatactt accggcaggt
     1561 gtcaacccct ttttttattt tccgcagtta catttaaatg ttccacattc tgtctgctcg
     1621 caggtacatg ccccggctcc tacaataccg atctgacctg ccgtacattt gtgttcatca
     1681 ttgtatacac agtgacaggc ttcacatgca acattgattt tctgcgaagc actgcccatg
     1741 gaacttttcg ctccctgtgt ggttcgttcg gtaaaatccg tacagcaggt ttcctgtact
     1801 ctggtcgcat tttcaccgcc tactttaata tcacctttac tgcagtacat cccgtcattg
     1861 tatacacatt tctgtgccga acatactaac atcgccatga tactacctcc ttttatgatt
     1921 ttgggttaca aagctgttaa aatcaaacga tgccataaaa ccactcctgt gcaagcacat
     1981 cgtggtttca ggcttttgac cctggcatct tacaaattgt ttccccgcta aacggttctt
     2041 acaatttccc ggatagtatc tgctgttttt tcttttttat tcacaacggt tcactgttct
     2101 ttttctggat ttcccgtatt tttttccagg ttctggcaaa atctttccga agcggatatc
     2161 catattcctg ataagtgatc gtcttgtcct tcgttctttc ttcaaaggaa aaataaagat
     2221 cacgggtata tccgtctaca tctgtgctgt ctgcagaagc atcattttct gcagccggaa
     2281 tatttttcag ttcctgtccg gttttttctg tcagctcttt tttataaata cggtaaagct
     2341 cttttgcatc ttctccggtc accacaacat cgtcttcata gagataagaa agacggatgc
     2401 ccgtcagttt ctcttctgca tactgcggat catttgaaag aatcggatag caaagctgct
     2461 gataccagga ctgcttccac agtttttcct ctgccgcatt ccagacatca tccaggataa
     2521 aatagcggcg atattttacn nnnnnnnnng tctgctccag atccaggtgg ctttcaatat
     2581 cacttattgt ttcaagactt tgcatctgat tttctatcac atagaggttt ccataaattt
     2641 ctgcaccaag accgcctctg ctgcacacag acatcgcttt aacctcttct tttgccggca
     2701 tccagcgatt atatcccggc cagtcaaaca ccagactgca gatcagcaaa atccctgccg
     2761 ccagtgtgat gccggttccg atcttatgag aaaagaccag ttttacatct ttgtgataaa
     2821 taaattccat cagcatacca agcaccagaa cgccgaaaat gaatccaccg aacaaccagg
     2881 aatactgatt tgatccggtc ataccgtatg ctacaatacc aatagccagt gcccccggaa
     2941 gtaccatcaa aatcttcagg atctgctctg ttttcggaaa tgccagtgcc ataccgccgc
     3001 attctgccct gtggtgcgta cacatccaga gatctgccaa aaagaataca acaatgccgc
     3061 caagtgtcat gcatgctgct ccaaaccacg gcgttccatg ctgaatcgcc tgtgctctcc
     3121 ctatcataat cccgattggt gtcgtatatg caattttcca gagctggatt gcttttaatg
     3181 cagtgtagaa tccggtttca atataagcct gggcgatcat agtcaccgcc ggaccataac
     3241 cggcgatcac tgccatacca aagacagcaa tcagtgtatt gccggtaaaa agcatcgcaa
     3301 aagcagctgc actgtagatc agcagaaatt ctatgatatg cagcacgctt cctttcagaa
     3361 acggcaaaaa taacagagaa agtgataccc cttttgtagc cgcaaccagc atggcaagga
     3421 tctcacacac cagaaaaatc ccgacaaaca tcaaaaatcc tgtcatatat ccactcagaa
     3481 agagcttctt tctgcgaacc gggatactgt aatacaaatc catcttctct ttgctgtgta
     3541 cataagaaaa ttccgctgct gcgatcatag ccgctgctgc cgttacaacc agtgccatgg
     3601 caatgtttcc tggtgccaga agatcgaccg ccacacgatt tctccagata tcatttaccg
     3661 ttgtctgtgc atccagtgcg atcatcatca acaccggata gcacacgaag aaaagcacac
     3721 ttaaaaatac cagcatccaa tttcttctct tggcatcttc ctgcatcagt ttaagaaatg
     3781 aaatttttga tgtcataacc ggccacctcc gtttcgctga taaatatttc ttccagagtc
     3841 agaggaagaa tctccgccag aagcgggttt ttctcctgaa tggtcatcag aatctcctgc
     3901 tttgttcctc gtgccacaat gctcataacg gagccgcgtt tttcatgctg cagcacttcc
     3961 agttttgcaa ggatttcctc ttcatccccg gcgttttgaa atacgcactg tactttatgg
     4021 atgtgatatt tcatttcttc cagttctttc gacagcagaa tgcctccttt gtacagcagc
     4081 cctacatggt cacagatatc ttccagctca cgcagattgt gcgatgcgat gatcggtgta
     4141 aagtcccgtt tcatgatttc agatgcaaaa atgctcttca ctgcctgacg tacaaccgga
     4201 tccagtccgt caaaactttc gtcacatatc agatattttg ttccggcaca gacgccaaga
     4261 agaagtgcca cctgccgttt catgcctttg gaaaacgtgg acagcttcct tgtaaattcc
     4321 agtccaaatc cggagaccat ttcccggaag cgttccatat caaactgcgg atagatcacc
     4381 tgataatatt ctgccatcgt ttttgccgtt gcattctgaa aaaaataggc attgtccgga
     4441 agaaagaaaa tcttcgcttt cgcctgtgga ttttcataga cattctcccc gtcgatcagt
     4501 gccatgccgg tatccggctt caaaactccg ctgatcacac gcagaagtgt gctcttaccg
     4561 gctccatttg tcccgatcag cccgaaaatg ctgccttcct gaatggttgc cacaatgttg
     4621 tccagtgctt tgatctcacc aaacattttg tttacacccg ttacttcaat catgttttcc
     4681 ctccctgtaa
//
LOCUS       DAWDWH010000166         4634 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_7362_length_4634_cov_13.853025, whole genome shotgun sequence.
ACCESSION   DAWDWH010000166 DAWDWH010000000
VERSION     DAWDWH010000166.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7362_length_4634_cov_13.853025"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1239 a         1126 c          828 g         1441 t
ORIGIN      
        1 atgcgaaaaa tttcgaattt atgactattt ctcttttaca ttatttctct gatctggaat
       61 atttttcaaa aatccggata tactcagctt ccacttttgc aaaagactct acggtataat
      121 ccagctcgat atgaagcttg ggcggctccc cgtcctgctt ttcaagcagc ccgtccagat
      181 cataccagag catcgcatgg tcgatctctt ccagctgtgc ctgctcctgt gctgtcagat
      241 ctgatcccag aaatttttcg taaatcaaat gcagcaaata tgcttcctgc tcctgatatt
      301 ccggcaatgt tttcttaaaa ggagacggca catcggacat ataacattca ctggcgtcgt
      361 gaagcagaca ggcaagtacc atgcgatcag acattcccct tgccgccgct tctttcgcac
      421 agcagataca gtgctgtccc actgaccaga aggtctttac atggccgttt ccccgacaga
      481 tcaacgacaa tgcatgggca atatccggta tatcgatcag ctccggattc ggatccgtcg
      541 gtttaaaatg ttttccggta taggtagtga taaattcgct catcgtattc ctgctttctt
      601 tttgcaatca gcaccggatt ttggtgcatg atctttcaaa ccaggcaccg ctcctccggc
      661 tgctgcccag aagtacagac ttgccacgct gcagtacgga cttaatctgc ggcggtattt
      721 ttcaaacagt ttgcggtcga tttttctgtg atggtaaatc atccgcatcc cacgctgtat
      781 cgccagatca ccaaaactca gcacattcgg tctttgcata cagaacagca ggatcatttc
      841 cgcagtccag acaccgatgc ctttcagtga tgacaattcc tcaatcgctt cttcatctga
      901 ttttttccag atgccttcaa tgtcaaaact gccgtctttt actttttccg caaaatctgt
      961 gatatactcc gctttccgga aagatatccc aagcggctgc agatgttcca cacctgcatt
     1021 taatatcgtg tccgcatcga ctgtcccaag ataatccttc attctctgcc agatcgtagc
     1081 ctgtgctttg gtcgagatct gctgaccgac aatatgatga accaccgatg agaacagatc
     1141 atcgtcaacc gttcgctcaa tcatgccaac ctgattgatc acctcagcga gacgtttatc
     1201 tttctgtttc agatagataa gttccttttc tccgtattca aaatacatat cctatttgat
     1261 tctcttcaat tcatattctc ttgttccaag tccgatcttt tctgcctggt caagggttgc
     1321 tttccactcg atattcggat tggaatcttt gaaatgatcg tggtggcaat gccatcccgg
     1381 ctctgccaga tgttctccaa gctgactgtt tgcaatcggt gttgcattca ggcaggcatc
     1441 tgcacatgcc tgatccagtg cgaccggatc aaagcttgcg aacattccga tatccggaag
     1501 gatcggtgca tcattctcac cgtgacagtc acagttcggg ctgatatcct gtaccagtgc
     1561 gatatggaaa tgcggacggt cgtgacagac tgcctgtgca tattctgcca ttttacggtc
     1621 gagaagctca tttgcactgt catttggatt gtataccgca tcaaaactgc aggctccgat
     1681 acagcgtccg catcctttgc atttatcata atcaataact gcttttttat ttacataagt
     1741 gatcgcatcg cttccgcatt cttttgcaca gcggcggcaa ccacgacaga cttccgtatt
     1801 gattgccggt tttccgcttg catgctgctg catttttcct gcacggcttc cgcatcccat
     1861 accgatattt ttaatggctc cgccaaatcc ggttgcctca tgtcccttaa aatggctcag
     1921 gctgataaaa atgtccgcat ccatgatcgc atatccgatc agagccgttt tgcagtattc
     1981 tccgtttaca accggaactt ctacttcatc cgttcctctc aggccatctc cgatgataat
     2041 ctgacatccg gtcgtagttg tattaaaacc attgagattt gcacagtcca gatgttccag
     2101 tgcattcttt ctgcttcccg gatacagtgt attacagtct gtcagaaacg gcattccgcc
     2161 ctgctccttg cacaaatctg caattacctt tgcataattt ggtcgaagga aagccatatt
     2221 tccaagttca ccaaaatgca tcttgatcgc cacaaacttt ccatccatat caatatcttt
     2281 gatgccggcg gcaatacaaa gttttttcag cttgtccagc tggctggtcc ctaccggaca
     2341 gcgaaaatcg gtaaaataaa cagtagattt tcccatgtca gttcctcctg atatattttc
     2401 ttccgtaagt ttcccgtacc tttgcatcct taatgccaga ctctgtaaaa aaacgtgttg
     2461 agtctgtaca gaatccttac tttctataaa agaataacac atcaagtaca cttgaagtca
     2521 agtacctgtt tgtaagtatc cgattttttc ctgcttctca ggctgccgca cctaagactc
     2581 tgcgaaaaca acagagccga tactgatttg aatgctctta agctatttct tttttcattg
     2641 ccttatagcc gattaccact gtccatacat aggcacatgt tttaggattg tcagccacat
     2701 ccagcggaaa gatttatcat tcttttcttt tgcactttta taaaacagaa caatgataat
     2761 caacccaatc agtgcgaatg gaatgttacg gtaaataccc caggaaagcg gagcatctgt
     2821 ggcaagccag ttattctgag gcatcatgca caaaacattc tgtacattgc cagatttata
     2881 ttatcaatta ctatattact tccatgatat aatctcataa tgtaccccca ttttttttta
     2941 cataagattc tcagatcttc taatactgtt tcaaaatcca gcgtatgttc tacatcataa
     3001 ttttctttga taaaagctat tcctttaaac tgaaaaagat actgaaaagc ttctttactg
     3061 ctaagtctat gcctattggc aaattcattt atacatgcaa cagtaaaacc aatttttctt
     3121 ttctgttcac tcattaaaac aactttcttc ctaaattaca cttaaacgtg tctggttatt
     3181 tactatagtt taatcacatc atcctacttt ttctgtgtga tctgtgccgg agagtgcagc
     3241 ctggatttct gacttgtttc ccgcagcaga tgctcttttt tcgtaaagcg gacattcaca
     3301 atccgcttcg ctacttgctc atgaacgcag tcgctgctct gcacacttga agcaggggaa
     3361 tgcttctctg cgttcaaaca atcgtattgt aattatagca taggtgtgag gtgcattgtg
     3421 tgtgcatcta tttatacgaa taatccatac tgcataaaag gcaatgaacc atttctgatt
     3481 cactgccttt ttctttacat atttttcctt tttatcttct gaatatcttc aggaattttc
     3541 ataatgtact ttattctgtt ctgttcattt cccttcgttc tatagcagca atttcttctt
     3601 ccgtatattc ttctattttt gttgctgtta cctgtttgat atgttgcttt ttcaggtgca
     3661 gatatacgaa ttctcgaaaa atagtataga acactgacaa cagtggaata aaaacaagca
     3721 tacctacaat tcccataatg tttccaccga ctgtaacggc tgccagcacc cagatggacg
     3781 gaagacctac agattcacca acaacatggg gatagatcag atttccctct atctgctgca
     3841 gtaccagaaa tactattatg aatagaattg cctgtttcgg gcttagcata aagattaaaa
     3901 agcttcctac cgcacacccg atgaaagccc cgaaaacagg gatcaatgcc gtaaacgcaa
     3961 ttaaaactcc aatcaggagt gcatatggca tccgcaaaat actcagtgta atgacaaaca
     4021 tacatccaag aatcacggct tccagacact gacctgtaag aaagtttgca aatgtccgat
     4081 acgtgagaga acaaacttta aggaatgcat ctgctttttg ttttggaaga aaagcgaaaa
     4141 atacttttct gacctgtaca tgcagctttt ctttctgaaa aagaatatag caggcaaagg
     4201 aaaccgcaat gaaaaaggtt gttaatccac tgatcaggga acccactgca gacattgtcg
     4261 tattcatcat atttcctgca ccgtttccaa gaaggcttat tgcccatctg actgcctgat
     4321 ccagattaaa ctgcagctga tctgtcagtt ttataatttc ctgattatta tggaaaaatt
     4381 ctcgaatcca actttgcata tgcgggataa aatcagcgat gctcgtcatc aaagttccca
     4441 ttgttcgtgt aagctgcgga atcacaccaa acattaccaa tgagatcaca ccaactacta
     4501 atacaaccgt aaggagcaga cttatcggtc ttgacagttt tttaactatc ttattttctt
     4561 tttttgcctt tttaaaaatt gtcctttcca aaaagctcat tggcacattg gtcagaaatg
     4621 cgatagctcc acca
//
LOCUS       DAWDWH010000167         4625 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_7374_length_4625_cov_16.295842, whole genome shotgun sequence.
ACCESSION   DAWDWH010000167 DAWDWH010000000
VERSION     DAWDWH010000167.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7374_length_4625_cov_16.295842"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1161 a         1108 c          884 g         1472 t
ORIGIN      
        1 gtgcgtccat gacaccaata ctgcttgcct ttcggctatg cagctacttt tattatatct
       61 ggttttttta gtccggttaa ggacagttcc ttctgttttc cggatacgga aagatatttc
      121 cgcaggatat taaatgtgcc taccgcatct gcattgtatc ttactccgtc agttacatac
      181 atgccccgtt cttttcggtt tgatgcttct gcatatcttt ttgatacttc tggtgataac
      241 gggctgcact ggctggtata gctttcttcc tgctttatca attgtattcc ataccgcttt
      301 agtttatatt ccagcatgat gtacagcctg ttatatggca ggctgtgaag cttctggttg
      361 gtcttatgtc ccatatcttt ttctttacgg atatttcgaa tgtctcccac aactacacag
      421 cggatatctt cttttctgca gtattctgcg atccatctgg ttactttatg taggtaatcc
      481 ttcactgcat tctgaggtct cctgcgctgt ctgtgacgga agctgtttat accataaatt
      541 tcctttatgt gtttttttct gataatacca gtcaggatat ttttccagtc ccagctcttt
      601 ataatgatgc cgttcatagt tgcagatatt ccagagtttg gaagctgcat aacacatatg
      661 ccctatgata ttggaatatt ccggccggat ttttacagat gttctgtgtg acagcagcat
      721 ccctctccct cccctcagac agtaacgcgg accggtattt acagtccgct ttaacttaat
      781 acgacttgct ctgatgttcc atataacggc ggatattttc ctcagataca gatccgatcg
      841 tttccacata ataggaatgg ttccacagct ctcccttcca tagctggttt cttatttccg
      901 gaaaacgttc gaacagtttt ctgccggtaa tccccttcag atacttgacg attgctgtta
      961 tggacagctt tggtggagct gttacaaagc agtggacatg gtctccttca ccacattcaa
     1021 ataaatgaac agtaaagccc ttatcctcag caatctgctg caccagttcc tttaggtatt
     1081 cctcaatttc tgagtttaat atcttccgtc ggtatttcac tgaccatacc atatggtagt
     1141 tgatattaca cacgcaagtt ctataatgta ttagattttc tttcatacat atagtatagc
     1201 ataatgcacg gaaatgtgca atacaaagtc gaacatattt tcgtctgatt ctatgccggt
     1261 tacggcatta atgtcactgg agtcaatggc agggagatta ttctattatt ttcaatatgc
     1321 agacatgcac actcctgcac tttcatctcg gtaattgaat tgccgaggat tcccgcttat
     1381 tgtcctaaat gattcttcca ttgaacgcac acgaaacctg gcatgcactt ttgctttatt
     1441 ataacataaa tcttaaagaa taccagagcg aatcccgaca cggatacaag cttgttgctc
     1501 gccgtcttta gaagcgaaag tcctctcaca tctgatttag caaacagaaa acgcttcaga
     1561 tgcagctccc tgtcacatct gtgactttgg ctctgcgtct aaagcgtctg gctgattttt
     1621 ctattttctg ttttccggaa gatgttctcc tgcttcaatc ccattcaaaa agaataccag
     1681 aacagcgacc acgcatccgg tcagtacctt gatccatttt gccattttat tctctccatg
     1741 gtaaaattat cgattactcc aatacttttt tgtaaaagtc aaagcagtcg aaggttgcaa
     1801 aataatcttt cggtgtctct gcacggcgga tcagttttgc ctctccgtct tctgtgagca
     1861 gaacttctgc agatttcaat ttgccgttgt aattatatcc catggaaaat ccatgtgcac
     1921 cggtatcatg gataaccaga agatctccca tatctacctt cggcagcata cggtcgattg
     1981 caaacttatc gttattctca cacagagaac cggttacatc gtacatatgg tcgcatggct
     2041 gatcttcttt gcccataacc gtgatatgat gataagcacc atacatcgcc ggacgcatca
     2101 gatttaccgc acacgcatcc acaccaatgt attctttgta ggtgtgcttt tcgttgattg
     2161 cctttgttac cagacagcca taaggtccca tcataaaacg gccaagttct gtgtagatcg
     2221 ccacatcacc catgcctgcc ggaaccagaa tctcattgta tactttctcc acgccttttc
     2281 cgatcacgcg gatgtcattt ggctcctgat ccgggcgata agggataccg acaccaccgg
     2341 aaagattgat aaatccgatg tgtacaccaa cttcttcttt caggcgtact gccagttcga
     2401 acagtttctg agccagcagc ggataataat catttgtgac cgtattgctt gccaaaaatg
     2461 catggatacc aaactcttcc acaccttttg ctttcatgat acggaatgct tcgaagatct
     2521 gttctgttgt cataccgtat ttggagtcgc cagggttatc catgatgtca ttgctgatct
     2581 taaagattcc tcccggattg tagcggcagc tcatcttttt cggcagcttt ccaacagctt
     2641 tctccacttt ttcaatgtgc gtgatatcat ccaggttgat gataccgttc aaagatgctg
     2701 cataagcaaa ctcctgttcc ggtgtatcgt tggaagaaaa cataatgtca tctccggttg
     2761 caccgatcgc tttggagagc atcagctctg tcatggaaga acagtcgcag ccacagccgt
     2821 attctcttaa aatctggatc agaaacgggt ttggcgttgc cttgactgca aaatattctt
     2881 tatatccctt gttccatgca aaggcttctt ttaaagcttt cgcattttcg cggatgcctt
     2941 tttcatcgta aagatggaac ggtgtcggaa attcttttac aatttcctga agtttatcgt
     3001 aagtcacaaa tggtttttta ttcatcggta cgttcctttc ctgttcactt gcatgaacac
     3061 ctgcagaata tgctgctgac agattccttt ttatatctgc aactttagca cgctgctctg
     3121 tcagacaaca atatgtaaaa aatataatac ggaagccttt gtttgtcaat acctgcatgt
     3181 atgcactttt gaattattcc agagcctttt cactgatatc tgcaaagaaa ttttttatcc
     3241 gatgtttttt atcaacaaga ttattatact gcaaaattgc ataatcattc aaaataccgc
     3301 tctgtttttc attgctccac tgctgaaatg tgccatctgt tccctgatag aagttcgctg
     3361 tcacaaccgg atactgctgc tgcagttttt ccagccagct ctgataaccg gtcttttcaa
     3421 gtccggcagt attcatcagc agaccactca aataattggt actgatcgca tcgacttctt
     3481 tttcctcgat gtcgtaattc gcccacatga caaacggcac ttcgtagctg gttttcagtt
     3541 tgtccgttgt atcgtttcgc gtggctgtgt cttctcctaa aacacgcaga acagagttgg
     3601 tgatatattc tgcaggctga tgatcaccaa acatcagaat gatggtcttt tcatcctcat
     3661 cctggaaata gtcgatcaga ttttcaaaag cctcatcggt ttttttgatc agagtcagat
     3721 atttctcagt tgcctcgatc tgaacctgat gaaccgaacg cagatcttcg ccggcatcgc
     3781 tgaactggat ctcatttgtc agatcctcat attctttgct gtagcctccg tggttctgca
     3841 tcgttacttc aaatgcaaac agtggactgc cttcctcttt ttcttcataa agctcgatca
     3901 gcttgtcaaa agcagactca tcatccaccc aaccacgcag caacgttgca tccggaaagt
     3961 ctccggtatc ccggaaatac tcgctgtcaa agccaagata ctgataaact ttgtcccggt
     4021 tccatccggt tgaataataa ggatgaagag ctgctgtctg atagcccagt tttccaagct
     4081 gtgatgcaag ggacggcatg gctccgttta aaaactgctg atacggaaca cttccggtcg
     4141 gcagaaatgc cacggacggt attctttttc agggaccgcc agaacggcat gtaatcttcc
     4201 gttgtgttaa agtctccata gacagaaaga tctgaaaatg cctcattcat gattacaaga
     4261 atatttggct gatcttccat atccgttttg tcggtatggt tctcttcgcc tccggatgaa
     4321 acggatgttt cctctgcaat cttctgtgcc gcatctgtgg aatatccctg cggcttttca
     4381 atatgcagat actgcaagtt ggcaagatac gctaccataa aaccattgtt tcggtaaagg
     4441 acatttggcg taaacagaat atcatccagt ccaaaaattt cctcacagtt ttctgtctgc
     4501 acatagctga caaaagcaaa catcagtacc aaagatactg caacggagag caggcgtttt
     4561 ttcacatttc caaagggaaa gcgcatcttt tctccgagga tcgcgagcca cagaaatccc
     4621 ataag
//
LOCUS       DAWDWH010000168         4570 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_7476_length_4570_cov_9.238538, whole genome shotgun sequence.
ACCESSION   DAWDWH010000168 DAWDWH010000000
VERSION     DAWDWH010000168.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7476_length_4570_cov_9.238538"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    282..291
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    641..650
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1426..1435
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3057..3066
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3918..3927
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1203 a          932 c         1152 g         1233 t
ORIGIN      
        1 gatgtggatg tgctggaagg tgtcagcaca gaagattcgg tgcgtatgta tctgaaagag
       61 atcggaaacg tgccgctgct gaccaccgag caggaggtag agcttgcaaa gcgtgtggaa
      121 gcaggagatg aagaagcaaa gaaacagctg acggaagcca atcttcgtct ggtagtcagc
      181 attgccaaaa aatatgtcgg tcgtggaatg ccgtttctgg atctgatcca ggaaggaaat
      241 atgggactaa tgaaagccgt tgacaagttt gactatacca annnnnnnnn ngacatggtg
      301 gatccgtcag gcgatcacca gaggtatcgc agataccgga agaacgatcc gtgtgccggt
      361 acatatggta gagaccatca acaagacact tcgtatgacc agaacgctgc ttcaggagct
      421 tggccgtgaa ccgaccccgg aagaagttgc agagagactg aatgtctccg tgtcacgtgt
      481 aagagaagtt ctcaagatct ccagagatcc ggtatcactg gatactccga tcggagaaga
      541 ggatgacagt catctgggag actttgtcga ggatgattcc gcactttcac cggcagattc
      601 cgcagcattt tccatgctca gagcagagct tgcaacagca nnnnnnnnnn ctggagtctc
      661 tgacagacag agagcgtcag gttgttaaac tgagatttgg tctggaagac ggaagagcaa
      721 gaacactcga agaagtcgga aaagagttca acgtaacacg tgagcgtatc cgtcagatcg
      781 aagccaaggc tttgcggaaa ctgcgtcacc cgtccagaag taagcgatta aaagattttc
      841 tgatctgaga gctgaagtcc ggcaaaccgc agacaggaaa taaaagaggg cacaggattt
      901 tactcctgtg cctttttcag ttaaccatca atacgttttt tgaaagcagg cagctgctca
      961 cggcgaagcg gcagggcagt actggtcgga tttaacaaaa aaccttttgc atctttcact
     1021 aggaacttct ccagatcatc aaatttcacg acaaccggac gaagtctttt cttcggatca
     1081 aacctctgaa gctcgtatcc gtctgtgaag atagcctgat acatctcgcc gttctgattg
     1141 ttgacatacg gaacacggat gttatttttt ttctttggct gttcttcttc tgtttctgat
     1201 tctttggctt cttcgatgtt tccgtcttcc gtatttccgt caggattttc tgctttactg
     1261 tcagcatctt tttcgctttc gtctacaata ataccgctta agaaacggcc gcgtaccaga
     1321 ttggcaacca gttcttcgtc cattgcccgc agatcagctt tttctttttc ttcctcgctg
     1381 accggacgac gcatctcctg caggaaatac agggaagtga gcatcnnnnn nnnnncatca
     1441 gctccggatt gatgacagga cgctgttttt ccggaagttc gtccggatta tgtcttctgt
     1501 gaacgatcgc ttcaagagcg agagaggttt ccgtatcatt gtcatgcagt accacgccgt
     1561 tgacaccgag agcgtagagt gtaccataga aatgaaggaa ctgctctgct gctactctta
     1621 cggataccac cgggattttt tcctgctcat tttttgcaac aacataagca tgtgctgcct
     1681 cagcgtctga gaagatatgt atctggtcat cgaaagattc ttcgttacag gttacataag
     1741 gatgcttggt ggactgggag aaaataacga acatctctgg aagtccctga agttttttga
     1801 tgataaagct gttatctatt gccatttgtg tacacctcat attctttctg ttatagtgat
     1861 tttgggatgt gaaagatgcc ggtatttatg caactttcac aattattgag accctactga
     1921 cagtcattgt accatatttc ccaataaaag aaaatataaa aatttatgat atttcgttga
     1981 aaaacataaa gaaatccgcc attgactttc gaaaaagaaa agaatatgat gaaagctaat
     2041 gaaataagga aggaattgta aaaatgaaaa aaggaaatct gagaagttcc gcattgcttt
     2101 tactgacggc atgcatctgg ggcgtggcgt ttgttgccca gagcgtgggg atggagtatg
     2161 tcgaaccgtt tacgttcaac tttgtgagaa gtattctggg agggctggtg ctgatcccgt
     2221 gtattttttt cctgaagcgg gaaaaaaagg caaaggcaga aaaagtcagc gaagaggaaa
     2281 aaaaagcaca gcgaaaaaca ctgctggccg gcgggatctg ctgtggtgtc gccctgtgcc
     2341 tggcaagcaa tttccaacag atcggcatcc aatatacaac cgtcggaaaa gccggtttca
     2401 ttacagcgtg ttatattgtg atcgttccgg tgatcggact ttttatgaag aagaaatgca
     2461 gtccgatgat ctggctgtcg gtgcttctgg cagttgtggg tttgtatctg ctttgcataa
     2521 acgaacagtt ttcaattgga tacggagata ttctggtatt tgtctgtgcg attctgtttt
     2581 cagtgcatat cctgatcgtg gattattttt cgccgaaggt ggacggtgtg aagatgtcct
     2641 gcatccagtt tctggtgtgc ggtctgttat ccggcattgc gatgctcttt gcggaacagc
     2701 cgcagttatc cgcagtgctt ctggcgtgga aaccgatcgc ttatgccggt atcatgtcct
     2761 gcggtgtcgg ttataccctt cagatcgtcg gtcagaaagg gatggatccg accattgcat
     2821 cgctgatcct cagcctagag tcctgtattt ctgtgctggc tggctggctg ctgcttggcc
     2881 agaccttaag tatacgggaa ggacttggct gtgttgccat gtttgcggcg atcattctgg
     2941 ctcagcttcc ggcgaaggag tcttgagtgc atttttgcga tactcccgcg gtgtgcatcc
     3001 catttcggat ttgaaaattt tagaaaaata gcagggactg gaaaagcctg tcagcannnn
     3061 nnnnnnaaat atcagtgata cttttgttgg tgttttgcag aagcggcagg cttttctgta
     3121 tgcggtaatg cagcaggtag tcaaaaagcg attcgttcat atgacttttg aaaaagcggc
     3181 agcattcgct tttacagata ttgatctgag cggcaacctg ttcgagcagg attttttcct
     3241 gataatggtc gtgcaggtag gagagcagca ccagcagacg ttcccggtca cgctgtaccg
     3301 caggaagcgt ttcagtctgt ggctcagtat tctgatacag ggtcagccag atcctggaga
     3361 gcaggatttg gatctgaaaa tcccagcacg gagccttgga agaagaaatt tcccagattt
     3421 ttgtcaggtt tgcaaggatt tctgcctgcc atgcgacatc cggggaaaat ttgatggaag
     3481 aaaaacggtc gtccagtgtc agcggtgtga cataggctgt ctggatgcgg ctggattcga
     3541 agccataaat gatacgtggg tcaaaggtcg tggaaatata aacacagtct tccttattat
     3601 acatgtgtcc ggtgtgcggc atgttggcat tgcagaaaag tccctcgccg gtggtgagat
     3661 gataagtttt atcatttacc tgataattga tgtctccgga tacgatcaaa gtcagctcaa
     3721 tctccggatg ccagtgccag aaaaaggaac ttcgctcata agaaaacagg ttttcatggc
     3781 ttacatttac cggaaaagca aacgttccgt ggtcttttag ttcacgttgt ttctgattgg
     3841 tctggatttg catagaatac ctcaatataa ttatagtttt tattatgata cttgttgatt
     3901 ttttctgtaa ttctcatnnn nnnnnnntag cgtgaacgtc aaggacacag agccggacgt
     3961 aaccgggaat gtttttgagc tgcgattttt acagaagaag cagttcgtga cagtatacat
     4021 ttaataagaa aaaggagaag tgacatgaaa gcaaaactga ttgaaaaatt tgtatccatc
     4081 tatggggaag gtggagaaat ccgttcattc ttttcaccgg gacgtgtaaa cctgatcgga
     4141 gagcatacag actacaacgg aggacacgtt ttcccatgtg cactgagcat cggaacttac
     4201 atgatcgtcc gcaaacgtga agaccgtacg atccgtttct attctgaaaa cttcagcaaa
     4261 ctgggcgtga tcgagacaag cctggatgat ctggtatatt ctgaaaaagc aggatggacc
     4321 aactatccga agggagttat gtgggcaata gaaggcagag gatacaagat cccgaccggt
     4381 atggatatcc tgatctacgg cacgatcccg aatggttccg gactttcttc ttccgcatct
     4441 ctggaagtac tgacaggact tgcagtacgt gatctgtttg gattcgaagg aatcagcatg
     4501 atcgatctgg cactgatcgg acagtattct gagaacaact tcaacggatg caactgcggt
     4561 atcatggatc
//
LOCUS       DAWDWH010000169         4566 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_7482_length_4566_cov_15.229883, whole genome shotgun sequence.
ACCESSION   DAWDWH010000169 DAWDWH010000000
VERSION     DAWDWH010000169.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7482_length_4566_cov_15.229883"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2896..2905
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          997 a         1119 c          896 g         1544 t
ORIGIN      
        1 gggagtgcaa cgcacggaac aatccgtagg catcatagtt ggggactttt gacatacaca
       61 ggataacgtg atcctccttc cttctgttat gctgtgcttc agacagtgtc aaatctgcct
      121 ttctgttaac atgcccttgt aattatatat tttatacaag ttttttgtgg gtttccatgc
      181 aatattatac gttttgcttg cattttttta tgatatccac attttctact gaaatgcctg
      241 gttttttaca caaacaggct tccgatgaat catcctgtat tgattttttg tatatttcag
      301 gactccctga cagcaaaaaa cgacagagga attacttcct ctgccgcctt tattctttat
      361 tcttcttgta tacagaattt tttagaatcc tgcaactttc tctgctgcat actcaaggat
      421 cagagctgca tcgcctgtgt ctgcaacacc gtcaccgttt acatctgctg ctgccgctgc
      481 atctgcatcc agttctgtca gttctgcact tgctgccaga agtgcggtac tgtcggctgt
      541 tgtcactgca ccatcgttgt ctacgtcacc taacagtctt gcttctgcaa gtttcagtgt
      601 cagtgtacga actgctgcgt ttacttcatt ctgaactgca tcatcgttgt cataaactgc
      661 ctgtgcatct gcttttacat ctgcaagtcc ttcgatggtt gctgctacat aagcatcgct
      721 atcttccaga acagctgctg ctttttcaag cattgctttc agagccgctt tgttgccttt
      781 cttttccaaa gagatcactg catcgatcag atcgctgtaa gctttgttta cttcttcaac
      841 ggttctgttt ttgtttgcta caacagcttc tgctgctttg attacttcat ccagttttgc
      901 aagagaattg cttgtgtaat ttccatcctg aagtttcttt gcagatttga tcaggctttc
      961 cagtgaggaa agatctgcat ttttcactgc tttgctcagc tcgttcagga ttgcagttgc
     1021 tgctgcatct acggtttcct gatctgcatc ttcgtttgca agaacggctt taccgtttgc
     1081 tactgcttct tccagatttc caaggctgct gtatttgtct gcacgtacca gcactttttc
     1141 agcttctttt atggagattt ccagatgtgt tttctcaaca ccctgttcca ggctgttcat
     1201 tgctgccaga agatctgtca ttgcgtttgt tgtctcaata acggttgcat ctgcttttgc
     1261 tgctactgct tttgctgcat tcagagcttc ttctactgct gcccagcttg cttttgtata
     1321 atcagaagca ttcaggtctt ctaccatagc gatcactttg tttaaggatt caacgtttgc
     1381 agaagctttc tgtacgctgt cttttgttac gctctgatat gctttcaggt tgccaagtgc
     1441 tgtgctgtag tcttttactt ctacaccata acgtctctgc atcatgttgg agttcatgat
     1501 gatgttcagg ttgttgatcg cactcttctg tacatcgccg aggcagatga tgttgttgtc
     1561 tacatattca cctttgtaag tcagagtctt gccatcgttg cttacggtta caacatcggt
     1621 tgttgccggt acgctgaatg ttccaggatt gaaccatccc tgacgatagt tcagatagat
     1681 ttcttcaccg tttacaagga ttcttccgtc atctgcaacg gttgctgtat agccttcacc
     1741 aagtgatgtc gttaccgtct ttgttccttc tgcagacggt gtaaattcat tccattttgc
     1801 agaatatact ttaaaagatc cgtacatcag ctgaacgttg atcgctacct gtcctctttc
     1861 atcgaatacc ggtgctgcat cctgcatacc gcttacgata ttctgtgctc tttctgctcc
     1921 gcccggcata atcatgtcat ttcctgcatg catggacttc atcggagtgg aggaaccgcc
     1981 gttccagtca gtcatgatca gaccgtcaaa tccccattca ccgcgggcga tgttggtaca
     2041 cagatcgtaa gaatctgcag tcggtacgcc gttgatcagg ttgtaggatg tcataataga
     2101 catcggacga gattctttta ctgcaatttc aaaacctttc aggtaaattt cacgcagagt
     2161 acgttcgctt acgatcgtgt caactgcatt acggttactt tcctggttat ttgctgcgaa
     2221 atgcttcagg catgcaccaa cacccggtgt ttcctgaacg cccagtgtgc aggctgctgc
     2281 cataacaccg gatacaaccg gatcttctga atagtactca aagtttcttc cgcagagcgg
     2341 atcacggtga atattctgag aaggtccgag cagaagtgtg atgttcattt catcaacttc
     2401 tttaccaaat gcaacaccga tacgttttac aaggtcggta tcccaggtct gagcctgtac
     2461 ataacttact ggccatgcgg tacaatactg gtacagtgtc tgtttttgat cgcttccttc
     2521 aatggtcgca tcaaatttct gtgctactcg tacaccgcca gggccgtctg caagaacgat
     2581 gcccggaatg ccatactgat cgtaaacggt tgtctcacct gctgcacctt ttaccgtaga
     2641 agagttgctt ccaacgatcg gagaattttc attggcaacg ccccatccgc ttccgcagtt
     2701 cagagccgcc agttcttcta cactcatctg tgcgacaaat tcgcccatcg tagcttttcc
     2761 ttcaatgaca tcttttaaag tagaatcttt cttttctacc acttctactt tttcgtacgg
     2821 ctgtgttgct tcataatcag catctgttgt gtaagtggtt acttcttcat tttcatatgc
     2881 agatgcatgg tcttcnnnnn nnnnntggtc ttctctcttg atttcagaag catcaacttt
     2941 gataaccgga gctgcttctt tttctgcttc ctctgttgga taactgtagc cctcttccgg
     3001 tttgctccat tctaccagat cttcgccgtc cggaacttcc atctggttgg aaagctgttc
     3061 agtcagtgtg gtgtcatcca cttcaatggt tgcctcaaca ctggtatttc tggaagaatc
     3121 accgacacgc acatagtatt ttcctttatc cagaatataa gctgcctggt cttcattata
     3181 atatgccatg tcttttgtat cataagaaat cgtcagaatc tgcgattcgc ccggtgcaag
     3241 ttcatccgtt tttgcaaatc cgccaagttc ctgatattct ttctctgcct ctgcagaatc
     3301 cggtgcggaa taataaacct gtacgatttc ttttccgctg taagtgctgc cggtattggt
     3361 aacttttact tttacggtaa ctttatcggc atctgctgtc acagactgtg tctcaatttt
     3421 gaaatctgtg taggaaagtc cataaccaaa ctcgtatttc ggtttgatat taaaggtatc
     3481 aaaatagcgg tagccgacat aaataccttc gttataaact tctttcttga cgtttctgtc
     3541 tgcatttgca aaggtatctg acgccggata gtcactgtag ttttctgccc aagtatctgc
     3601 cagttttccg gatggggtta cggctccggt cagaacatcc agaagtgcgt tgccgccttc
     3661 ctgacctgcc tgtcccatca gaagcattgc atccagtccg ttaatctcgt tatagaattt
     3721 tgtatccata ataccgccga cattcagaac aacgatggtc tttttaaagg acgctgccac
     3781 acgctccaga ttttcacgtt cgagatccgt cagataataa tcaccaatat cataagattc
     3841 tccgttgtaa gttcccttcg tccctttcag tgtacggtcg gtaccttcac cggcatttcg
     3901 gctgatgaca tagattgccg tatcggaatc tgcagaagca tctgccagct cttcatcact
     3961 gatctccatc tcagggaatt tgaagcagtt catcgtactg tttccttcaa ttttgtgctg
     4021 tgcatcataa gcatctgcgt atgcatcgag catatcagag tttacgatat catagccggc
     4081 ttttttaaag gtattataaa tgtggatatt ataacgttca ttcagatcta catcccagga
     4141 accacctccg gaaagtcctc cgttgaacgg gtcaccggaa ccaaatccac ctttgatggt
     4201 acgaaccgca ccgcttccga acagtgccag tttttttgtc ttcaaaggaa gtgtatggtt
     4261 ttcattttcc agcagaacca taccctgtcc ggctgcctct cttgccagtg ccgcattgtc
     4321 aatctcccgc tgtgtgatct catcacttgt actggctgcc aatgcagaag aacacggtgc
     4381 tgtgaccata ctcactgcca gtgccgctgc catggcactt ctgatttttc tgttacgttt
     4441 tttcattctt tgctcctcct tttgcccgtt tatacgtttt gctctatgat tatagatttt
     4501 acacaatttt cttttgattt tccatgtaaa attatacgtt tttcttgtaa atttttatta
     4561 tgtatc
//
LOCUS       DAWDWH010000170         4558 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_7496_length_4558_cov_12.852099, whole genome shotgun sequence.
ACCESSION   DAWDWH010000170 DAWDWH010000000
VERSION     DAWDWH010000170.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7496_length_4558_cov_12.852099"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          982 a         1138 c          932 g         1506 t
ORIGIN      
        1 cactgcttat tgcttttcgc aattgaagca ggggacttac ttgatttggt taaatcgtga
       61 aatcatggaa atatattaca aggagaactt ttcaaatgaa cgttacaaaa actgctgcct
      121 cgcagcacag taaaacttac gaaatgaccg tcaccgcact gatgacagct gtgacctgta
      181 ttttagctcc gctgtccatt ccgatcggtc cggttccgat ttctttcaca aaccttgcta
      241 tttatctttc tttatatctg ctcggatgga aacgcggcac gatcagttat cttgtctatc
      301 tgttgatcgg tctggtcggc gttccggtat tctccgggtt cacaggcgga atcggcaagc
      361 ttgccggtcc taccggtggc tacatcatcg gtttcatcgc catggcagtg atcgccggac
      421 tggtgatcga caaatgccat aatcgctggc tccagctgct cggcatgatc gtcggaacgg
      481 ctgtatgcta cgctttcgga actgcctggt tctgtatcag tgccagctac accgttggtg
      541 ccgcactgac ggtatgtgtg attcctttta tcccggccga cctgatcaaa atggttattg
      601 ccatgctcct cggaccggag attaaaaaaa gaatccgcta aaaaagaatt tcaggaaaaa
      661 aatttaaact gaaatgttgt tttttcgttt ttgtttttgt ccgaagaaat gatctggatt
      721 ccgtgatcga tcacttcttc ggatttttct atatactgat aatggtactg tgtgcctgcc
      781 agatcttcaa agatcagctg atctcccacc tgaagttctg acaggttttt tgtaaatgca
      841 gcaattccat tttcggtcat gccctccgta tcccacggca tggatgtccc atctctgcaa
      901 agtgagaatg atgcagtatt tgcatcaaag gtatttaata tggcatactc tgtattttgt
      961 gacggaatct gcaaaaatcc aaccaccact ttaccttcca cactcaaaaa ggtatcccgg
     1021 tccggatttt tgacgttttc cagcaagatt tctctttcct gttttgcttc atccagcagt
     1081 ctggctgccc gtttctgatc ctgtattcca aaatgaaggc aggtgacggc tgctgccccc
     1141 aaaagcagca gcacgatcat cagccctggc aggaaccaat ttccggtacg tatttttaat
     1201 tgtttctccg cacattttct ttgtatttct gccatataaa tctcccccca agtccctttg
     1261 tcttcgcagt cattcagttg caggcatttt aaccttcacc gaatgattac tttattttca
     1321 tggttttttt ctggaatccg actttggctg tcagtttttt ccttaacttt ttctttgctt
     1381 ttttcggtac cacaaagacg gcttttttat gaataccctt aaatgcattt ttaccgatct
     1441 ttgcaatcgt cactgacttg atattgatct tcttcagatt tttattcttg tagaatgctt
     1501 ttgccgcaat gctctttaca ttcttgccga tcgttaccgt tgtcagcttc ttgttgcctt
     1561 tgcatacatt tgcaccgatt gccgtcacct tgaactgtct gccgttgatg cggaccgttg
     1621 acggaatatt catcttcttc gctcccggct ttttgtagga taccagcatg acagcctttt
     1681 tggatgtcac tttatatctt gccttgccaa ccggataaat ctttccaata actacatcct
     1741 tcggcttatc cgatggagtc ggagacggtt tttccgttga cggtttttcg gtcgtcggat
     1801 tggatacttt tttccagatt gcatacagtt ctgtcgtttc tgtgatatag aatttatcac
     1861 ccggcagata cgtagttccg cttccgtctg cctttgtatt ccagctgaca aattccatgc
     1921 cgttatactt aaatccacag tttgcagcct gaacggtatc gtatttttta tacggactgt
     1981 tcggatctgt catcgcaccg cttccgccgt ttgcatggta gatcacctga agaccttttt
     2041 cctcttcgat ctgccctaag acttctgctc cgtaagtgaa ggaggccgtc attgccgcac
     2101 cgtctgccag gttccatctg ccctgcacaa atccatggcc gtctttggaa acagtcagtt
     2161 tgttgccttc gatcgcggct gttccggaaa ctgcatgcac ttccagatcc accgtatcca
     2221 gatcattgag gatgatcttc tgatcatttt cggtcacggc acacagctgc agtgccactt
     2281 ctacgccgtc tgcatctttc aggaacagat ttttattggt gtaggaaatc cactgtttct
     2341 gtgtggtttc ggttgtatct gcacttaatg caatatccag atttttaata ttacccatgc
     2401 catcattgta tacgctgttt cttgcagtct ctttatttcc gactgcgtcg cttactgtaa
     2461 tctctacttc ttttttataa taaccactgc caatgttcac ttcataagca aattcatttc
     2521 cttcgccaag tgctgtccca agatccccaa gcgttttttc tcttgcaagc ggttttccgt
     2581 ccaccgtaat ggtcagtttt gcatccggct ccgtgattcc gctgatcgtc agcgtgccgt
     2641 cttctgcaaa tccagatcca ttggtcgggg aggtcagcat cagggtcggc ggtgtcgtat
     2701 cgatgttaaa tacaaaactc tgatcgaaac cgtcaccttt ttcattcttg ccgtaaatct
     2761 gaagggtatg atctccgtct gccaggtctt ttaatggaat ggcaatttct gatgtatgtt
     2821 caaacgttcc tgtttttact tcttcgccgt ccagcaccca ggttccggac actgccatat
     2881 cggcttttgc cgtgaagttg acagaagatg ctgcatacgt gtcgtacatc acaatctttt
     2941 ctgtgacatt tccatcctca tctttttcat tttcgcttct tccaacctga taaaccttct
     3001 gatccgctgc aagtgtgatc tttgctttct gcggttcatt taaggtaagg acttcgccaa
     3061 acgtttcttt tccgtaaacg ataccggtca gattgccgtc accgtctaaa agtttgttaa
     3121 atggtgtcgc actgatcacg tatttcgttc cggcagtcag tccgatggtc tcaacggttt
     3181 cttttccgtt ttcatcgaca cttctggatt cgctgctgcc gccaagcacc gcatagatct
     3241 gattctcttc gtcttttacg gcagaaacat tgtaatccga aagttcttct ttttcgccat
     3301 ttgcaccgac ttcataaacc ttaaacagtg caccctgaca tttgtcatcc gttaccgtat
     3361 caagggaagc acggatctgc agatcgcctg cattttccgc atggatcgct gtgatcgtct
     3421 ccggctggct gctgttcgta taggcaaatg catttgcagt atcaacgatt ccggttgtga
     3481 cttcttcctg actgtacaca agtcttacat aatattttcc gctttccatc gtatccggaa
     3541 ttgtgatcgc agcctttcct tttgcgatat ccgctcctgt cagagaagcg atcggtgtac
     3601 cgccgttttc ttccgaatcc ggattttcca gaatgtaaat atcaagtttc tccagatcag
     3661 acattttgtc gtttcctgtc catgtcagat tgatcttatt atctgcactg ctgtagttct
     3721 gtttatccat ctgaactgtg gtgacctccg gaagcggatc tacgccatag aggatcagtt
     3781 cagaagaact tgctgttttt atcgtataag ttttatttgc cagtgacgga tcggtaaagg
     3841 agacaacaag ggttgcttct ttcttctctt catcataagt aaagttggca tttgcagtct
     3901 cattctgttc atcggttttg tcattgtcat aatactggat cggatatggc gttccattct
     3961 catcggtaac tttgagtcct tcttttcctg cttttgcctc ttccagtgat tctgctgaaa
     4021 atgcaatact cagaacctgt gccacgccgt cctggaaatt gccaagcgtt acgttatgcg
     4081 tggttcttgt aatatcagag cgaagactgg agctgatttt tccgtctgca tctgccaggg
     4141 tgctgttttc gcttgaatcc ttctggtaaa tattcgggcc gatgctcatg taaagcatat
     4201 cctgtttttc ttcatcatag ccaacggcgt accatctgcc ggaaataccc tctgtgcttc
     4261 ttgcttcttc cagatactgc ggataggttg gatttacttt tgcctgtgag ccaaaggaga
     4321 ggtcgccgcc ataggcataa gaaataccgg ttgagaatcc gagcactttc agtgcacccc
     4381 agatacgctt tgtgttcagt ccaagtgccg caccgccgat ctggataccg ccgatcagcg
     4441 ggatcacatc cggaatgctg acaatcgcct gtgcataagc ttcaaatgct tcctgtgcat
     4501 cactgctggc atcaatcaca atatagccct tgccatcgat gatgcccata tagttgat
//
LOCUS       DAWDWH010000171         4553 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_7508_length_4553_cov_9.894842, whole genome shotgun sequence.
ACCESSION   DAWDWH010000171 DAWDWH010000000
VERSION     DAWDWH010000171.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7508_length_4553_cov_9.894842"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4201..4210
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1097 a         1205 c          803 g         1438 t
ORIGIN      
        1 taaattacaa tttttcacaa agatttcatc gatatccaga tcctctggtg ttgtaatctt
       61 gatattataa taagagcctt ccaccagttt tactttttga ccttccattt tctccagtac
      121 cattgcatca tccgtgattc ccattttggt atggtcctcc tgcatcattt tttcataagc
      181 acctaaaata tcaggatacg caaaaacctg cggagtctgg atgttccaaa cttcacttct
      241 ctttaatgtt tcttttgcaa ataacgcttc gtcagccagt ttcagtgtat ctttcgaagg
      301 aactgctgcc acacacgcac cataagtgca taccgcttct tttaaccgct gcagcatttc
      361 ctgatctaca aatggtcttg ctccgtcatg gatatagatg taatcacatg ctccggcagc
      421 tttcagtgca ttatatacag aaagatagcg ttcttccccg ccttcaataa tatctttcac
      481 cttttgcaaa tgataaactt ccacaatctt ctctctgcaa tatgcaatct catctttttt
      541 tgtaaccagt atcacttcat ctataaattc gcattcttca aattgtctga gtgcgtaata
      601 caaaactggt tttccaaaca gatccatata ctgcttctgt acctgactgt gcattctttt
      661 gccgctccct gctgccagaa cgatcgccgt gtgcttttct ttcatttagc gttcccccag
      721 tttcagattc ggaactgcat tcagatccag tccggatctt gttcctgcca gatattcata
      781 atatcctgcc actccgatca tcgctgcatt atctgtacag aaaatcgggg acggatggta
      841 aaatttgata cctttctttc tacaggcttc ttccattgcc atgcgaagtg ctgaattgga
      901 ggcaactccc cctgcaatgg caaatttatc aaatccatat tcatcgatcg cccacatgga
      961 atgttctacc agaacatcaa caactgcttt ctgaaaggaa gctgccacat ctgcttctac
     1021 gatcggctgg ttcttcattt tgcatccatt cagatagttc aggactgctg acttcagacc
     1081 gctgaaactg aaatcatatg gtgcatctgc aacttttgct tttggaaatg aaatcgccag
     1141 cggatttcct tcttttgcaa gtttatcaat ctttggtccg cccggatagc caagtccgat
     1201 cgcacgggcc actttatcaa atgcttctcc ggccgcatcg tctctggtgc gtccgaggat
     1261 ttcgtatttt ccgtaatctt ttaccacaac caggtgtgta tgtcctccgg atactaccag
     1321 gcatgcaaac ggcggctcca gatccggatg ttcaatataa tttgcagaaa tatgcccttc
     1381 tatatggtgc acacctacca gaggcagatt tttagcatag ctgatcgcct ttgcctcggc
     1441 tactccgaca agcaaagccc ccaccagtcc aggtccatag gtaaccgcga ttgcatccag
     1501 atcatccagt gtgacgtttg cttcttcaag agcttctgtg atgacctgat ttattttttc
     1561 aatatgcttt cttgatgcaa tttccggcac tacgccgcca taaagtttat gcagttcaat
     1621 ctgagaggaa ataacattgg acaaaaccgt tcttccgttt ttcacaacag aagctgccgt
     1681 ttcatcacag gaactttcaa ttcccagaat cagtacatct ttcttttctt ccatctcgta
     1741 tctatccttc ctattatatc aagcccaaag gcattgattt accagacttt tctgtcgact
     1801 gtcattcctc ttcaaatgac agcactgtac tttttccgtc ttttcccggg taagcatttg
     1861 ggaaaatttc acgcacgcca tccataaatt cttccggacg taccagtgac ggactgtaat
     1921 gtgtcagcca taactctgac acttctgctt ccgctgccaa ttttgctgct tctttgaaca
     1981 tcatatgttt atgctccagt gccttagact ttttatcatc ctcgccatac ataccttcgc
     2041 agacaaacag atctgagtgc tctgcattct ggatgatgga tctggtcggt ctggtatctg
     2101 tcgtgtaagt cagtttgatt ccctttctgg cttctccaag aaccatatcc ggtgtatagt
     2161 gcacaccctc aaattcgaca acattcccct tctgaagcgg attccagcag cacagcggaa
     2221 tatttgccgc ttttgcccgc tctacatcaa atttccccgc acggtcaatc tccatagtat
     2281 atccgtaaca aagcacattg tggtttacac ggaatgcacg gattcgataa ccgttcatct
     2341 caaaggtttc ttctgttccc tggatttctt tataatatat ttcaaaaggc agctccggtg
     2401 caattgtccg aagtgcattc accacacgtt ccagcccctt tggaccgatc aaagtcagcg
     2461 gttcatgacg ttctgcattt cccatcgaca aaagcagacc cggaagcccg ctgatatgat
     2521 ccccgtgata gtgtgtaaaa cagatcacat cgattggttt aaaactccac cctttttctt
     2581 tgatcgcgat ctgtgttcct tcaccacagt cgatcagaat actgcttccg ttatatcgca
     2641 gcatcaaggc tgtcagccaa cgtcttggaa gcggcatcat accgccgcat ccaagcaaac
     2701 aaacatcaag cattcttttt tccttactct ttcttcagtt tctttttctt tcgtttcttc
     2761 tttcttttct acagcagctc tgtctcttct tttatttctg gttttaatac aaatgctgct
     2821 gtcagtttat cgtaacaagg ttcacacaga tcgaagctgt ggcattctcc gtctttttcc
     2881 gaaggatatc cccagatttt ttcaatatga atatattctt ccatgtctct tccaatcgta
     2941 tccacaattc ttttcccgca gctgttgcag taaatcttat tctcttttct catcgtatcc
     3001 tccccaggtg ataccccttt tgtatgcttt ttctgatttc ctttgcgaca gcaacagtat
     3061 aacgaaaagt attcctttcg tacagtggga attagaggaa tcctagtggt tgccgtattt
     3121 ccacatgatc agtgcatctt cttttggttt ctcgtaaaaa ttcggtcgga taccggaacg
     3181 ctcaaaccca agtttttcat ataaatggat agcaggtgca ttgccaaccc gcacttccag
     3241 tgtgaaattt tcgattccgc gttcatatcc acgcaaaaat aattgctcca gaagttcggt
     3301 ggcaacccct tttccgcgaa acttctccac aactgctacg tttgtgatat cagcctcatc
     3361 aaaagactgc aacagtccgc aatatcctgc cagtatttcc ttttcatatg caaccagata
     3421 gatcgtctct ttctgacgca gtgcatcttc aaatcctttt ctgctccatg gccgggaaaa
     3481 aatctgactt tctgttcttg caactgcctc cagatcttct gcctgcattg cacgtatttc
     3541 aagcacgatt ctgttcttcc ctttcttttc gctctctctc tgcctgagaa agacgcagat
     3601 aatccggttt atgttccatt gcactttctg ctcgtccctg tacatagtac tgtgctccaa
     3661 gaactgcaac cgcagatgca cgctgtctgt tcatatgtgc cggagcaaac gaatacggaa
     3721 ttttcatcac ttccgccaac gttttgcggt aaaccggaac gccgtcgcca agaaaaataa
     3781 cttctcttcc aagtgcatta agtttttctg caatctctgt gacagaaacc gccatctgat
     3841 tttccagtat ctggagtttt ccatcgcaga attcgtaaat gccggtatag acctggtttc
     3901 ttcttgcatc catgatcggg cagacgattt tatctgttcc ccataaatca taggccagtc
     3961 cgtctaccgt tggaaccggt atgatcggtt tgtcaagtgc cagccccaga ccttttgccg
     4021 tggcagaacc aatacgcaga ccggtaaagg atcccggccc ggcagcgatt gcgatcgcat
     4081 ccaggctgtt taagtccaga tttatcatat ctccgatttc tttcagcatc ggaagcaagg
     4141 tctgagagtg tgttttttta taatttaccg tgtattctgc aagtaaaata tcatcttcca
     4201 nnnnnnnnnn ccacaacagc aaccgatgcc accagccctg agctgtctat tcccaaaact
     4261 ttcatgtttt cctcctaaag ctgtgttttc ttttcctccg gatctgaaat cgtgatcttg
     4321 cggtaatcaa atcctttttc cagatttttc tcgatttcaa tggcgatcac atccggcggc
     4381 agaatctcct ggatcagatc tgcccactcg atcaagcata caccctcacc gtaaaaacag
     4441 tcctcgtagc cgatctcttc catctcttcg atatcaccga tgcgatacac atcaaaatga
     4501 taaaacggca gacgaccgtc ttcgtacatc tgcacgatcg taaaggtcgg gct
//
LOCUS       DAWDWH010000172         4552 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_7511_length_4552_cov_9.526796, whole genome shotgun sequence.
ACCESSION   DAWDWH010000172 DAWDWH010000000
VERSION     DAWDWH010000172.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7511_length_4552_cov_9.526796"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    249..258
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1592 a          791 c         1201 g          958 t
ORIGIN      
        1 gatcttacgg cactgactgc ctgggggaat ctgatcacca cgcaggatac caggagagtg
       61 accacgattg aagaatttaa aaataaaaaa tttcgctatc agctgtcaaa agcgacagta
      121 gaaatcgagc gaatggtgat ccgggtagaa aatctgttta tcgaaagttc ttccctggag
      181 ccgacacttc tggagcgggt ccgcatgaat ctggaaaaaa tcccgcaaat gcagaacgaa
      241 gaaccagann nnnnnnnnct ggtggaataa tctgggcagc gatttcatcc gtctcaatca
      301 gaattatcag gattatatgc gtgagctgaa cagtgtgaaa gcggagcaga tgatgaaaac
      361 gcaggaattt ctgatcttca aggaccggct gatcgaatat ctgcgaagtt tcgtaaaaag
      421 cctgcagatc aatgcgtcgg cgatcgagca gtacctgaag aatacagaag aacatcagat
      481 aattgctata ctggagaaag aagcaaaata tgaagtctcg attcctcaga ttgatgtaga
      541 gctgacaaaa gaaatgatcc ttgataaagt caccggacgc tggcagagcc tgaaagagtg
      601 gttcatggcg acggaggatc agcagtcgga agtggtgcgt gtgtttgata cgacaaacga
      661 aatcataaga aaaatcacaa gatatgcagc aaggatcagc gaactgagca atagcggttc
      721 caatcgaaga gaagaatacc ataaactggc agtgatgttc tcaaaatgcg aggatatcta
      781 tgaggcacac aggctggcgg cttctgtttt cgggatcgaa aaaccgctgc atttaaaggg
      841 gctttctggg cgaaagacgg agagcatcaa cagcggtgtt tttgaagaag atccgcatgt
      901 ggtaacagtg cttccacgtg tacggaccta caaggaaaag gcaaaacgaa gcggcattgc
      961 agaccgcagc aaagaaaaag aggcagtgcg tctggagacg atccgcaggc tggaggaaga
     1021 acagaaactg cttcgcagct atattaaaga tggaaaaatt tcgtttgcag aactgccgga
     1081 gattgagccg catgtaagag atgtttttct gctgtggctg tcaaaagcac tggagaataa
     1141 gcattttcgc gggaagacag agagcggcga aatttattat atcgaaaatg cagatacgaa
     1201 agaaacctgt atgttgcatg caaaagatgg gattttacag atgccggcgt acacaatccg
     1261 gtttgaggag aaaaaatgaa tgcgttagaa atattactgg ggagaagatg gattttaaaa
     1321 agcagagaca gggaattgta ttatcggatg aaagatgaag caccggccat taagaaattt
     1381 ctgacggaga agctggggta tcagatgatc gttaatccat atctgataaa aatagagaaa
     1441 atgccggcaa agccggagaa ctggatgggg attctggaat ttaacgacaa gatagagtat
     1501 gtatttttct gtatgatcct gatgttcctg gaagaaaaag aagcgatgga acagtttgta
     1561 ttatcagaac tgacggaata catacaaggg cagtatcagg aagagcagat tgactggacg
     1621 gtctaccagt accgcagaca cctgatcaaa gtgctgaaat attgtgtgaa cagcggcatt
     1681 cttgatgtgg atgacggaag tgaagagaat tttgcgaaag acgggagcaa tgaagtgctc
     1741 tatgaaaata ccggggcgtc ccgctacttt atgcggaatt ttacgcagga tatcatgcaa
     1801 agagctttat ggaaggggat ttatcaaaac ataccgtgag atgacaacag gagaattttg
     1861 tgaaaatgta aagaattata tgctggaact gggactgatc cgggaagaga aagatatctg
     1921 gctcagtccg gcagtcggaa aagtgatcgg aaagtatccg gaagaattta tgaaaaatgg
     1981 aggcagtgat gaacagtaaa tggcagatat acagggtagg gctgattgat ttctggtact
     2041 acgacgaaga agagttttac tttctggatg gcaggatgct gcttcgcgga gccaatggtt
     2101 ccggaaaatc tgtaacgatg cagagtttta tcccgctgct cctggatgga aatatgcgtc
     2161 cggagcgtct ggatccattt gggtcaaaag caagaaaaat ggaaaattat cttctcgaag
     2221 aaggggatgg acgggatgag cggaccggat acctttatat ggagctcaaa cgcaaagaaa
     2281 gcgatgaatt tctgacactt ggcatcggta tgcgtgcccg gaaaaataaa aaaatggact
     2341 cctggtattt ctgcattacg gacggcagac gcatcggcaa agatctttat ctctataaag
     2401 aagaaaaaag caagatcacc tgcactatgc gtgaactgaa aaaccgcatc ggagaaggcg
     2461 gaaaagtgat ggaaacacag agcgagtatg cacagtgtgt caacagactg ctgtttggtt
     2521 ttgagacaca ggaagaatac agggaactgc tgaaccttct gatccagctg cgaacgccga
     2581 aactgtcaaa ggatttcaag cctaccgtga tcaatgatat cctgagtagt tcgcttcaga
     2641 ccttatccga agatgatctt cgtccgatgt cggaagcgat cgaaaatatg gatggcatga
     2701 aaacaaatct ggatacctta aaagaaagta tttctgcagc gaaacagatt gaaaaagtgt
     2761 acgatcagta taatgagatc gtactgtatg acaaagcact tctctataca aaagccataa
     2821 aagagtgccg ccaggcggaa gaaaaaacag ttcagactga aaaagcaatc ctggaaaata
     2881 cagcatctta tcaggcagaa aaagaacatt atgaagaact tgcaggagaa gaaaagctgc
     2941 ttcaggaaga gaaggactcc ctggcagata atgatgcagc caaattaaaa gaccgcgaga
     3001 ttaaaataag ccaggagatc agagagttac agaaaattgt tgccgataaa aaacagcagg
     3061 agcagacgaa aaacgaaaag cggatcgata cagaagaaat cctgaaaaaa caggaagaaa
     3121 agaaagaaga aaaatggtct gaaatcgaag aatatctggc ggaaatggaa tccgatatgg
     3181 atggcgttcc atttgatgat ttttctttta tgaaggaaga gctttgcgaa aagaaagaag
     3241 aggaatattc ttttgatgca catcggaaac tgctgaaaga ttacacgaaa aaagtagagc
     3301 agggaaagca gttgcttctg gaagaaaaag cggtgcagga gcgttacagc acgttccttc
     3361 aggatttgga tcagtatcag gatgagagaa acaaagcaga gagagaactt ttacagtacg
     3421 aaaatcagct tcatgagatc cgtcaggaac tgatcgagca gatttataaa tggaatcagc
     3481 aaaatgaaga actgcatccg gaggcgaaag ttctgcagga gatggcaagg cagattgaaa
     3541 actatcagtc ggacagtgac tactgggcga ttcgaaattt aagcaaaaaa gcgtatgatc
     3601 agaaaaacga ggctttatca gcagagaaaa cccagagaag ctggcagctt tcagaactta
     3661 aaaaagaata cgatgcgatc agcacggaac tgagacagtg gaaaaaccag aaagaaccgg
     3721 agccggaaag ggatgcggca gttcttgaaa acagaaaacg tctggatgaa aggaacatcc
     3781 catatctgcc gttttataag atggttgatt ttgatcagaa gctggggaaa gaaaaagcag
     3841 ccagactgga agaagcactt ctgcacatgg gaatcctgga tgcactgatc attgcaccgc
     3901 agtaccggga gcaggttctt cagagcgaag aaggatgctg cgaccgctat ttatttacgg
     3961 atgcagagta tgtcaggaac aaccttatgg attggctgga tatcgacaat ccggatcatg
     4021 atatgttctt atatcagaat gtatcgggtg ttttgtcagc aatcacatca ggaagatccg
     4081 atcaggcagc gacctggatt gatgaggacg gaaattataa gatcggagtt ctcgaaggca
     4141 cggtaacaaa acagtacaca ccttgtttta tcggttctac agcaagagaa cagtatcgcc
     4201 tggataagat cgcagaactg cagcagctcc tggaggagaa gcaaaaagaa atcgaaacag
     4261 aagaacagga aatcgaaaaa atccagagtc gtctgaaaaa actgtcggaa gaatggaaca
     4321 ctttcccaaa agaccatgac ctgaagattg ccgcaaaaga gtgtgaaaag caggaagaaa
     4381 aattaaagga tctgaatgaa aagatacagc agcagaaaca gcgtgtcgag acagagcgaa
     4441 agaagctgga tgaagtacgc ctgcgggtgc aggaagcctg tcagaaatgt tacttgaaag
     4501 cgagactgga tctgtttgaa gaagttcagg aagcactgaa agaatatacg ga
//
LOCUS       DAWDWH010000173         4529 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_7559_length_4529_cov_11.357175, whole genome shotgun sequence.
ACCESSION   DAWDWH010000173 DAWDWH010000000
VERSION     DAWDWH010000173.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7559_length_4529_cov_11.357175"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1110 a         1186 c          845 g         1388 t
ORIGIN      
        1 actcgtcgat atggaaaaag aatttgagaa atataacgaa aatgccacac tttcctgaat
       61 ttatcagcag atcggaattt ttccattcaa gaatgtttct gatgtacgtc gtatgttgca
      121 tttcctccgt ttctataaca aaaaaacaga cagcgaaatc gaacccgaaa tcactgtctg
      181 ttttttaatt tttacatttc agcgaagcgg acaagaatcg cataataaat cttatctaca
      241 ttcgcctttg ttttttaatc aagaatgatc agtgccataa ataccagatc ggtatctccg
      301 gtattggtca gtgcatgacc gtggttatcc ggtgtgtaag tcacatctcc tgccttaacc
      361 gggcgtacag ttccattatc atcataatca ccctgacctg ctaagatgaa ataagtttcg
      421 ctctcattgt gatgttcatg atagccaaga gaacatcccg gttcaatcgt tacttttgca
      481 tataatccgc attttccgtt gagttctttt tcaccgagga tacggtcaat gattacgtgt
      541 ccttttccac cacacatttt ttcaactact tctctctttg ttgtcatcat tacgccaact
      601 ccttttaaaa atgataaaat tgattttata atagcgattc agacataccc ttataaccga
      661 accattatct tctgctggca tctgaccaac aacccgatcg gttaaaagaa ttttgcaatc
      721 tcttctactg attttcttgt cggtctgtcc ggatctgcat cgctgtatcc cacactgatc
      781 acactgacaa tggactcttc ttttgggatg tcaaatgctt cacgcaattt atctgcatca
      841 cgcagtccca taaccagcgt agataagccc tgatttgccg cctccagcag aagattcata
      901 tcgtgcagac cgcagtcgta atagccccag ccgtttccaa gctcgttatc tgcagtgccg
      961 tctttctgaa aaccggcacg gtttaaaata acggcagata tcaggaaaac cggagcgttc
     1021 tgcgtttttt tgcgattgcc ttcacccagg gcatccagta ctgcctgtct tttttcttcg
     1081 gaaaccgcca cataataacg gatctcctga gcatttttcc aggtcggtgc aaggattgct
     1141 gcctcgatca tagcttttac tttttcttct tctaccggag tcgtcagata ctgacggata
     1201 ctttttcgtt tctgcatgac ttcccgaagt tccatttgtc ttacctcctt gcagctgctt
     1261 tctctgctgc atccactaca tgtccgatca gaacagatgt ggtaacactt ccgacaccgc
     1321 ccggtaccgg tgtgatcgca tctacgatcg gctctacttt atcgtaagct acgtcaccgc
     1381 agagtttgcc ttctgcattg acattgatgc cgacatcgat gatcacctgt ccggctctta
     1441 catagctgtc atctaccacg cctgcacgac ctgctgcaac gatcacgata tctgcctctt
     1501 ttacaactga cggcatatca acggttctgg tatggcatac ggtaacggtt gcattttttt
     1561 tgatcagcat catagctgcc ggcttcccta ctaccagaga acggcctact acaactgctt
     1621 ttttacctgt gcagtcgatt ccgtaatgat ccaggatctc catgcaggcc tgcggtgtac
     1681 aaggaggata tcccagatct ttgcctgtaa acacaccagc catggaaccg ttggtcatac
     1741 agtcaacgtc ttttgccgga tctaatgcct gttcgatctc gtcctgattt aaatgttttg
     1801 gaagcggacg gaataacaga acaccatgaa tactgtcatc tttgtttact tcatcgatca
     1861 ctgccagaac ctgctcctgt gtcgcatctg ccggcagcag atatttttta tattccacac
     1921 ccagtttttc gcagcgtttt gttgctcctc tttcataaga aaggtcgctt tcattttcgc
     1981 ctacacgtac aattccaagt gtcggtgtaa caccgtttgc ttttaatgct tctgcttttg
     2041 cgataatacg ctcgtttaaa gctgcaacaa cttcttttcc taataactgt tttgccataa
     2101 tgcttatcct ccgcctattt cagtcttcca agaacacttg caaagatctc atctgccatc
     2161 ggcgggtatt ttgccagcat agcatctgct tttgcgttca gttcttctgc atattctctg
     2221 tttttcatgg attttgtgtt gatatacaca ttcagggaag ctccttccag tgctgcttta
     2281 cagaatacgg cacctacacc tgcatcactg atcgcaaggg ctgaaccttt tgccgcaaat
     2341 tctttgatca gttcaatcgc ttcacagcat ttctccatga tctccatcgg tacggagcag
     2401 gcatctttca gcacaatctc cataacgcgt gctttctccg ccttctcttc ttctgtctct
     2461 cttggcatgc catacgcctt ggagagcggc tcaaatactt ccgcgtcttt ctctaccaga
     2521 gtcagcagtt ctttctgaag aacatcgcat tttgctttta actcctgcat ttcttcttct
     2581 acatccgcat attttttctt tccgacagtc aggcttccga ccatatttcc aagagctgtg
     2641 ccgattgcac caaccagtgc agatgcaccg ccaccgcccg gaaccggtgc tttggaagcc
     2701 agaacttcta caaattcatt acatggtacc gttgaaaatc ccattgtaaa atcctccttc
     2761 attttcttta atgtcccgat cgcaatatcc ccgacaactg ccaggtcttc tgcttcaaag
     2821 gacagcggga aattgtctga aaacagaatc tgggaggatg gtggaaattc ctcagcaaaa
     2881 actgtacgcg atgcccgttg ataaattcaa actcgtatcc ggcatcaccg tgtccgcaat
     2941 ttactgcccc cagtgcttcc attttgtttt taaattcctg caatttattt ccataagaaa
     3001 aagcaagccg catcatacat cttccgttaa actgacggta ataaacttct ccatgcggca
     3061 cttcccgata agtcagaaac tttccggtgc cttctgagta cacacccctt gttaaaaatc
     3121 ggattaccat gatctttggc ggaacgccat tctccatggc tccatatcct tcatcaaatg
     3181 gatttgcctt tcgaatggta cattccggcc attttacaag aaatgctttc tgcatcattc
     3241 gcacttcaaa ttcctgtgtc tcttcatgcc agggtactcc caccctggct gcggcctctt
     3301 ttggatcgat tgctgcaaat tcctccagat aatgttcaaa aggacttctt tctttagtat
     3361 ctttgtcgta tcttacctgc ataaaattct ccttttgatg ccgcatttat cttccgtctc
     3421 acatacgttc gacttgaatt ttaccgattc ggaaacagag atgcatctgt atgcggcagg
     3481 aaacatgccc ctacaaattc atccatatac tgcggaatcg tactgagttc aatatatgtc
     3541 atattagcgg caagatcata tacttttcgc tcagattcgg tagaacagag catcgcatac
     3601 gcacctgcca gtgatgtatt tcctacataa tgatacattt caaccggaat atccgggaac
     3661 attccgatat tgatcgcatt tgccatgttg ataccgcttc cgataccacc tgcaacatag
     3721 acattctcga tcatagagat atcaaagtca cagtaagaaa gcatggtgcg gattgcagag
     3781 aaaatagctc ctttcgcacg gataaagctg tcgatatcca cttctgtgat ttctacatct
     3841 ttgctcgatc ctgcttcttc gccttcaaat gccagcacat agctgcccat tccgtctgca
     3901 tcgcgtttca cgcggcggcc ttctctgaca aattttcctt ttggattgat gatcttgcag
     3961 cggaacagct cactgataat atcaatgata ccggaaccgc agataccaac cggttttgtg
     4021 ccttcattgc caacgatcgt cagcttcggc tccatcgttt ctgcatctat cgtacatgcc
     4081 tcgatcgcac cgtcggtcgc tcgcataccg cagctgatat ctccgccctc aaaggctggt
     4141 cctgcagaac atgcacagct gaacataaag tctgaattgc caaatacaat ctcaccgttg
     4201 gtaccaaggt cgataaacag agagaatggc tcctgattcc actgcatcgt tccaaagtct
     4261 cggtctgtgt gatctccgca gacataactg ccgatattcg gtgcaagaat gatatgtgca
     4321 tccggattta ctttgattcc aatatctcct gcaaaaatcg aattggtctt aaagaaggtc
     4381 ggaatgtacg gttccatgcg aaccggatcc gcattaactc caatcagaag atggttcatc
     4441 gtggtgttcg ctgcaaccga catacggtaa atgttcatcg ggttgatctt tgcctgacgg
     4501 cacatcgtct gtaccaatgg attgattgt
//
LOCUS       DAWDWH010000174         4460 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_7687_length_4460_cov_12.123269, whole genome shotgun sequence.
ACCESSION   DAWDWH010000174 DAWDWH010000000
VERSION     DAWDWH010000174.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7687_length_4460_cov_12.123269"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1219..1228
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1325 a          867 c         1216 g         1042 t
ORIGIN      
        1 gtcaatatgt gtggtgatat tatttacaat ctgaaaagtt atattcagaa tcagcgttcc
       61 tacagtgcac tgcgttaccg caagatcgta aaaaacaact atcgcaagtt tgtggtactg
      121 gtgctggtat ctaccatacc gacaggtatc attggttata tcggaaaggg aatggtggaa
      181 aaagcgagtg cgacactgat cgttccgggg atttgcctga tcctgacttc cattttgctg
      241 gtggtatccg agaaaacacc gaacggcaaa aagataccaa aggatatcag ttacggaagc
      301 ggatttttga tcggtgcagc acagggctgt gccacacttc cgggactttc ccgctccggt
      361 acgacgatcg cggcgtgcct gatgagcgga tatgacagac gttttgcagt tcgctattct
      421 ttcattatgt cgatcccggc gattctggga gcggctgttc ttgagatcaa ggatctggga
      481 agtgaaacga tcaccggggg catcgtactc aacggactga tcggagcgat cgttgcagga
      541 tgcgttggtt atgtatgcat taaaacgatg cttacagtcg tgcgtaagaa aaaatttatg
      601 ggatttgcga tttactgttt tgcagtagga gtgattgcga tcattgcaaa cttcctgata
      661 taacagaatc gattttacaa aggaatgttt tatatgcaaa aagactgtta cggtatggat
      721 tctgtaacag ttttttgtta tggagaccgc ggatcagctt ataaaatcca caaagcgtgt
      781 tttatctttg ctatagcgac gaaaaatgtg atatgacgag gaaaacagat aaaggggagc
      841 aacattttat ggcagaaaga aaaaaaacgg tgcaaagtac aagaagaagc aattctgcac
      901 caaagaaggg aaacaacagc aggaaaacga cagcaatgcc gaagaaagag actgcggtac
      961 cgaaaaaggg aaaggtcggc agaccaagaa aaacggatta tcagaaaccg gaaagaatcg
     1021 aaaatccagg aaatgaagga ttctctgtct gggaggaaat cctggtctgg ctggtgcttg
     1081 ctctgtccgt tctgatcctg atcggaaatt tcggtgtcgg cggtttcatc ggcaatacgg
     1141 taagccatat cagtctcgga gcatttgggc tgatggcgta tctgtttccg tttgcccttt
     1201 ttctgggagt ggcttttgnn nnnnnnnntt ttgcagttgt aaacaaggaa aaaagcatta
     1261 tgtgggttaa actgggcgga tgttgtctga tgtggcttgc catgtccatt ttctgcgaac
     1321 tgatcaacgg atacgataag agcagaaatc tgatggacta ttataagtac agcatcgagg
     1381 aacatacagg cggcgggatt atcggaggca ttttctgcaa agtgttctgt ccgtccgtcg
     1441 gcattgcggg agcttatgtg atcgtgatcc ttctggctgt gattgccctg gttctgatca
     1501 cgcagcgttc tctgtttcgt ggtatgcaaa aaagcagcaa aaaagtctac tgttctgcaa
     1561 aggaagatgc cagacgcaga atggagcagg caaggataca ccgtgaagag caggcacttt
     1621 accgggaaca gcagctgatg aatgaaaggg aagctctgga gcggagaaaa gaggcgtttg
     1681 aagaagaaaa gaaacgacgt cttgatcata aagtgaccgg tgtgacttat cagacggtag
     1741 gtgaaaatct ggtttcggag aaaagtgaaa ttccaagaga gatcatcgag gcacagccga
     1801 tcatcgaaga ggaaccgggc aggagagagg aaatctatga agaggagccg gaagaatcct
     1861 ttgattttct gaatatgctt tccattgaag gactggatga agaagaccat tctgatacgg
     1921 aaccgtcacc gcatccagtg gcggatcatg cagaagaatc cggcggttca acagaaacgg
     1981 aaaaagagaa aaaccggaaa aatccaagat cttccgcaaa agagcagaaa gtccagctgg
     2041 aaagtgtcag cgaggagatt gaagcggcag aaaaggtgca gaaaccacag tatgccttcc
     2101 cgccgatgga gatcctcggt cttccaagac gcggaaaaaa cggaaacagc agtgaggtgc
     2161 tgaaaacgac ggcccagaag ctgcagaata cactgcacag ttttggcgtg gaggctcatg
     2221 tcaccaatgt cagctgcgga ccttctgtca cccggtacga attgacaccg gagcagggag
     2281 tcaaagtcag ccgtatcgtc aatctggcag atgatatcaa gctgaacctt gcggcagaag
     2341 atatccggat cgaggcaccg atcccgggga aagcagcggt cggcattgaa gtcccgaata
     2401 aggaaagcag cgaagtttat ttaagagaac ttttggagtc atcggagttt caggaagcca
     2461 aatccaagct gtcaattgca gttggcaagg acttggcagg aaagattgtg gtggcggata
     2521 ttgcaaaaat gccgcatctt ctgatcgccg gtgcaacagg gtccggtaag tctgtatgcg
     2581 tcaatacgat catcatgagt ctgatctaca aagcttcgcc ggaagatgtc aagctgatca
     2641 tgatcgatcc aaaagtcgtg gaactgagtg tttacaatgg gatcccgcat ctgtttatcc
     2701 cggttgtcac cgatccgaaa aaggcagccg gtgctctgaa ctgggcagta gcggagatga
     2761 cgaaacgtta ccagcagttt gccacccagg gcgtgcgtga tctgaaaggc tataatcaga
     2821 agatcgaatc gatcccggaa gaacagcgga cagaagaaat gaagaaaatg ccacagatcg
     2881 tgatcatcgt ggacgagctt gccgatctga tgatggttgc tcccggagaa gtggaagatg
     2941 cagtctgccg tctggcacag cttgcaagag cagccggcat ccatctgatc cttgcaacac
     3001 agcggccttc cgtcaatgtt atcactggtc tgatcaaggc aaatatgccg tcaaggattg
     3061 cgttcaaggc ttcttccggt gtggattcca ggacgatcct ggatatgaac ggggcggaaa
     3121 aactgcttgg aaaaggagat atgttgttct atccgcagaa ttatcagaaa ccggcaagac
     3181 ttcagggagc gttcgtcaaa gacagtgaag tggaacaggt cgtagaattt ttaacacagc
     3241 agaataaccc agaaacatac aatgcagaaa tgcaggtgca gttatcgcag gtgacacagg
     3301 ctgcccagga tctgaaaaat agcggaagcg atgtagatga atactttgca gatgccggaa
     3361 agtttatcat tgaaaaagac aaagcatcga tcggtatgct ccagcgaatg ttcaagatcg
     3421 ggtttaaccg ggcggcacgc atcatggatc agctttcgga tgccggtgtt gtcggcccgg
     3481 aagaaggaac caaaccgagg aaagtgctga tgagcatgga acagttcgaa aattatcttg
     3541 agcaggggca ataagtattg aaatttttaa gggattgttt tatactttaa tgtagtgttt
     3601 tgtagaacct tgcgactgta tacaaaaaac acagtcaggt gtgcttttat aaaacaaaaa
     3661 atatggaggt atgatatgta caagatttta aaagcaggaa aacttgcagg caatatctac
     3721 gagatggttg tagaggctcc gcgtgttgca aagcattgtc tgccgggaca gtttatcatc
     3781 gtaaaaatgg atgaagtcgg cgagcgtatt ccgctgacta tttgtgacta tgacagagaa
     3841 gccggtacag ttactattgt attccagccg atcggagctt ccacagagaa atttaccaca
     3901 ctgaaggcag gagatacttt ccgtgatttc gtaggtcctc tgggacagcc gtctgacctg
     3961 tgcacagagc cgctcgaaga agtaaaaaag aaaaagatcc tttttgtagc tggtggtgta
     4021 ggtaccgcac cagtatatcc acaggtgaaa tggctgcatg agcacggcat tgatgcggat
     4081 gttattgtcg gtgcaaagac aaaagacctg gttatcctgg aaaaggaact ttccgaagtg
     4141 gcaggcaatc tctatgttac aaccgatgac ggttcttacg gaagaagcgg aatggtaaca
     4201 aaagtgatcg aagacctggt aacaaaagaa ggaaaagtat atgatcactg cgtagcgatt
     4261 ggtccgatga tcatgatgaa attctgctgc ctgacaacaa agaaactgaa cattccgact
     4321 attgtcagca tgaatccgat catggtggac ggtaccggca tgtgcggagc ctgtcgtgtt
     4381 atggtaggag atgaagtgaa atttgcctgc gtagacggtc cggaatttga cggtcataag
     4441 attgattttg atgtggcaat
//
LOCUS       DAWDWH010000175         4453 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_7703_length_4453_cov_13.721010, whole genome shotgun sequence.
ACCESSION   DAWDWH010000175 DAWDWH010000000
VERSION     DAWDWH010000175.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7703_length_4453_cov_13.721010"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1313 a          898 c         1187 g         1055 t
ORIGIN      
        1 gctttcgctg accagaatca cgcaccaagt ctcacgtgcg ttcgacttga attcaagttc
       61 cgggtcgaag aagcaaagtc tccttcggtc aggatgaatt tatggtggca tgcatacttc
      121 cttctcaaag cactataaca tattgataaa atattatcaa ggcaagtcca tgctcaaaaa
      181 acggaacatc catattgtat gtgaaaaatt taacacaaat atcgcagtta tctgacataa
      241 ctatggaaaa gtattttgtt tgttaaattt ttaattaatg gaaaaggcat tgtatataat
      301 aaataaataa gatataatta acacacaaaa tttgtcaata aatacaagag agtaggaggt
      361 atgatttatg ctggatcagt catattacca gaaagcggat gagatcattg ctttttatgg
      421 taagaaacca tcatccctga ttcctgtgat gcaggatatt cagggggtat atcgttatct
      481 gccgggagaa ttgctgacgt acattgcaaa agagattgga gtaaaagagg caaaggctta
      541 cagtgttgcg actttttacg agaatttctc ttttgaacag aagggaaaat atgtgatcaa
      601 ggtctgcgac ggaacagcct gtcatgtaag aaaatcagcg gcaatcctcg atgcatttat
      661 gaaagaactg ggactttccg ataagaagca tacaacggat gatatgcttt ttacggtaga
      721 gaccgtatcc tgtctcggtg cgtgcggact ggcaccgacc tgtacggtga acgatgaagt
      781 ccacccgaat atgacaccgg agaaagtagt gggattgatt acagaattgc gaggtgagtg
      841 ctgatgatcc ataacagaga cgagttaaaa gaatgtcgcc agaaggcaaa agcagatatg
      901 gagacggcag cctgtcgtat tctggtctgt tccggcaccg gctgtatcgc aaccggttct
      961 gaaaaaatat acaatcgctt tttaaaactt gcaaatataa caccgggcgt gagcgtaatc
     1021 tttgcaccgc acgatgaaga cacacacatt gggctgaaaa agaccggatg tcagggcttc
     1081 tgtgaacttg gcccacttgt gcgtatcacg aaaaacgatg agatcgttca gtatgtaaaa
     1141 gttcaactgg acgactgcca ggaattgttt gaacgtgctg tgctcggtga tgaggtagtg
     1201 gagcgtttat tataccgtaa agagaaaaaa gtttaccgtc atccgaaaga gattccgttt
     1261 atcgcaaaac agaccaggat tgttctggag aactgcggac gttttgatgc agaatccatt
     1321 gacgagtaca tggcatccgg cggtttctct gccctggaaa aagcgatgtt tgagatgaca
     1381 agtgaagaag tgatcgaaga gatcgataaa tccgggctgc ggggaagagg cggcggcggt
     1441 tttccgaccg gacgcaaatg gaaacaggtg gcacaccaga aagaaaaagt acgttatgtc
     1501 gtatgtaatg gtgatgaagg tgatccgggt gcttttatgg atggttccgt tatggaaggt
     1561 gatccgtaca aactgctgga aggcatgatg attgccggat atgcagtcgg ggctgccaat
     1621 ggttatatct atgtgcgtgc agaatatccg atgtccgttt ccagactccg ccatgcgatc
     1681 gcagagatgg aaaaccggaa tctgttaggt gacaacattt taggaacgga tttctgtttc
     1741 catatgcata tcaaccgcgg agcaggtgca tttgtatgtg gagagggaag tgcactgacg
     1801 gcatccatcg aaggaaaacg aggtatgccg cgtgtcaaac cgccaagaac ggtagaaaaa
     1861 ggactgtggg agaaaccgac cgtcctcaac aacgtagaaa cgtttgccaa cgtaccgaag
     1921 atcattctac agggagccga ctggtatcgt tcgatcggaa cagccggaag ccctggaacc
     1981 aaaacattct ccctgaccgg tgcgattgaa aatactggtc tgattgaagt gccgatggga
     2041 agtacactgc gggaaattat ttatgatatc ggcggcggac ttaaggaagg atctcagttt
     2101 aaagccgtac agatcggcgg accgtcgggc ggctgtctga cggagaagca tctggatgaa
     2161 ccgcttgatt ttgactccgt aaagaaattt gatgcaatcg taggatccgg cggaatggtt
     2221 gtcatggatc agcacacctg tatggtagaa gttgcccggt tttttatgag ttttacgcag
     2281 agagaatcct gcggaaaatg tgttccgtgc cgggaaggta cgaaacgtat gctggagatc
     2341 ctggaaaaga tcgtagaagg cagaggcgaa gaggaagatc tcgaccgtct ggaggagctt
     2401 gcggcaatga tccgcagcat ggcactttgc ggactcggaa agagtgcacc gcttccggtc
     2461 atcagtactc ttaaattatt ccgaaacgaa tatctggaac atatcaacga gcataaatgt
     2521 gccgcaaaag tctgtcaggc tatgcgtaaa tatgtgatca atccggagtt ctgtaagggc
     2581 tgcagcaagt gtgcgaagaa ctgtccggtt ggtgcgatca gcggaaaagt aaaacatccg
     2641 ttccatattg acccgaatat ctgtatcaag tgtggtgcat gtaaagaaaa ctgtgcattt
     2701 aatgccattt acatagaaga gtaggagggc agattatggg atttatgata attgacggta
     2761 gaaaagtaga atttaccgat gaaaaaaata ttctgacggt cataagaaaa gcaggcattg
     2821 acgtaccgac actgtgttac cattcagaac tttctacttt tggcgcgtgc agactctgta
     2881 cggtagaaga cgacagagga aggacatttg cgtcctgttc cgaagagccg agagatggca
     2941 tggtgatcta tacaaactca gaaaaagtaa gaaaataccg caagctgatc gtagagcttt
     3001 tgctggctgc acattgcaga gactgtacga cctgtatcaa gagcggcgag tgtgtacttc
     3061 aggaactggc acaccgcctc ggcgtgaaaa aagtacgttt ccagaataca agagaagaaa
     3121 gaccgcttga cttttcttct cctgcgatcg tgcgtgatcc gaataaatgc atcctttgcg
     3181 gaaactgcgt gcgtgtctgt gacgagatcc aggggatcgg tgcaatcgat tttgcttacc
     3241 gcggaaccga tgcgatggtc atgccggcat ttgaccggca gatcgcagag acagactgtg
     3301 taaactgcgg acagtgccgt gtattctgtc cgaccggtgc gatcacgatc cgtactaaca
     3361 ttggagaagt atggaaggca ctggcagaca aagataccag agtcgtggct cagatcgccc
     3421 cggcagtgcg tgtggcagcc ggtgatgcat ttggacttcc gaaaggtgaa aatgcgatgg
     3481 gcaagattgt ggcagctctg caccgcatgg gatttgatga agtctttgac accagttatt
     3541 cagcggatct gacgatcatg gaagaatctg cagaattcct gaaacgcctg aaaaaagggg
     3601 aaaacctgcc gctgctcacc tcctgctgtc cggcttgggt caaatttgtg caggatcagt
     3661 tcccggatta taaagaaaac atttccacct gccgttctcc gcagcagatg ttctccgcgg
     3721 tcattaaaga atattaccgc aatccggagc atgcagaagg caaaaagact gttgttgtat
     3781 ccattatgcc gtgtacggca aagaagatgg aggcagaacg tccaaacagt tataccaaag
     3841 gtgagaagga tacggatatt gtcatcacca caacggagct taccaggatg atccgtactg
     3901 ccggtattgc actcgataag atcgaagtag aatcctgcga tatgccgttt ggtattgcat
     3961 ccggtgccgg tgtgatcttc ggtgtgaccg gcggtgtaac tgaggccgtg atccgcagac
     4021 tcgttcctgg acatggaaat gccgtgatgg attccatcgc agaagccggt atgcgaggcg
     4081 aagaaggcat caaagaattt tccattactt atgaaggcaa agaactgaat atctgcgtag
     4141 ccagcggact tgccaatgcc agaaaagtca tggaacaggt acaaagcggt gagaaacaat
     4201 atcatctgat tgagatcatg gcgtgcagac gcggctgtat catgggtggc ggtcagccaa
     4261 tcccggcagg tccgagacat aaagaagcaa gagcaaaagg cctgtaccgt gcagacggaa
     4321 atatggcgat caagaaatgt accgaaaatc cgctgatgga tgttttctac aacggagaag
     4381 atttcaaagg aaaagcacac gagctgttac ataatccgtt ctaaaagaga gaccccctca
     4441 catatgctgt atc
//
LOCUS       DAWDWH010000176         4444 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_7723_length_4444_cov_9.192299, whole genome shotgun sequence.
ACCESSION   DAWDWH010000176 DAWDWH010000000
VERSION     DAWDWH010000176.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7723_length_4444_cov_9.192299"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          984 a         1087 c          905 g         1468 t
ORIGIN      
        1 ggttgtctcg gttccgtctg cttttgtaac aacgatatca tatttgaaga tacggccgtt
       61 tgcactgttc tgtcttggca gacaggaaag tctgctgatc gtctgaactt ccggcagtgt
      121 tacttcaaag taatgattgt gtgtaccgga aatgacatcc ggtgtacgcc agtttgtatg
      181 ccagaatgta gtgtcatctc cgtcgattgc attctgtgcc gatccttcgg aactctcctg
      241 gctgcaggca gatactgtcc agcccgctct ggaaagagca tttccgcttt cggatacggt
      301 ggtcggatct gcacttaccg tcagttcgtc taccagacct ttgaaactat tggtcttgct
      361 tccgatgtac tcaaccggaa gtaccagtgt ggcaactttc gaaccggttg tctttgtctc
      421 gttgtccaga gttgcggatg atacatattt attgtttaca taaagctctg ctttgtcttt
      481 gtaaccattg atggtcaggt atacccattc atctttcggc agttcatagt taaaggaata
      541 atcatatcct tctcttgaga atccgacttt tccggtattc ttctggactg ctttgatcgc
      601 ataggtgttg aattttgtgt tggtctcgca gagaacctgc tcactgttgt cgctggctgc
      661 atctctcttg acccacatgg aaatgctgct tcctgctgtc tttccgcttg tcggtccgac
      721 catgtcaacc ggagtctcta cgtagctctc gccgccgttc agtttcagtg catttccgct
      781 cttgcctgct acttcttcta cgttcttctc gcttacagaa tcattgttgt ttccggattc
      841 atcgtaaact ttttcatcat cgaaggagta atcgatcaca tcattgccta cggaatctgc
      901 ttcgtagtat ggatttgtgt tcggtgcagt atccactttt tctgcaacgg cacgcatctc
      961 gctgtaagtc ctgtcggttc cttcgcccca catcttctca cttaaggacg gcagcggctg
     1021 gaagaaacgg tcaaagtcat cgtattctgt gataccgttt ccgcgtgtac cgatctggtc
     1081 attccagatt gcataagtac cgccgagtac ctgtttgtct ccggctctta aaacagtacc
     1141 gctgaaatta ttcggtaccc agttgttgta aaggttctgg gaattcaggt agtctgaata
     1201 atatcctgca gaaggtacaa tgtaaaggct tccttccagg gtattgatca ggtcatatcc
     1261 caggtcatac atattcttcg gatttgcata tccggtgttc cagatgttca gctgtacatc
     1321 tttggatgca accggggttt taccgttttt gttggaaagg cttccccaca tacggacgtc
     1381 acgtcctgtt ccctggatgt acttgatcat ggaatcagag aagcttctga aatattcatt
     1441 tccgccgttt ccgtggaatt catcggttcc gatatgtacc gtggtgtctt tgtcaaatac
     1501 cggatcatca ccttcgaagt actgtgccca tacggattta gctgcatcca gtgcaccatc
     1561 ttttcggatg tccagttctt cgatcaggta tctgtggttt ccgcctgcct ctttggtcat
     1621 aaggttcggg aaggctcttg tgatcgccag ggcatgtgcc ggcatatcaa attccggaat
     1681 gatgttgaca cccatggttc tgctgtcttt aatgaaagaa cggaactcct cttttgtata
     1741 gtatgtatct tctgatgtca gatttggaat tttagattcc agacggaaac ctgcatatgc
     1801 attctctacc gcttcatcaa tcgtagcgta atcttccagg aagatcaggt tgtcggacag
     1861 gtgcagatgg aagttattca tcttgtacca tgccatgttc tttgcaaatt cctttacggt
     1921 attgaaatca aaagcttttc ttccgacatc gagcataaac ccacgcactt cgtacttcgg
     1981 ataatctctt gtgatacctt tcggaatcgt tgttttgttc tgttttaaaa tctgcagtgc
     2041 actgatcacg ccccagtatg caccggttgc tttttctgct tcgatcttta cgctgtcacc
     2101 gatcgtcatg atatagcctt cttcgtcaag accctggtct gtgctgttca gtgtcagata
     2161 aaaatcacct tttttcacat cggattcgct gccttttacg gtctggatat cgtttcctgt
     2221 gatatctttg taatcttctg caaactgttt tgccgttgct tccagtccgt ctgcataaac
     2281 aatcttactg ctgctctgga tcacgaaatc accggtctcg ccatgccact cctgcagtgc
     2341 cgggatgaca tccggttttg cattggctcc ttctgcatct gtataacgtc ccggaaccgt
     2401 aatggtaaac tctgcggact gtgctttatc tgtgccgtct gcattgctga tctggtaaaa
     2461 tccttttacg gtcttggtct gcagcggttt gtaaacggtg ccgtcacttc cgatgatctg
     2521 ctcataatct gcatcaaact ctaccgtata tccttccgga acggtcggca tcggaatctt
     2581 tgtcgtatct gcatccacaa caggtgctgt catagcagca gcgatcttct gtgcatcggt
     2641 tctctcatcc ggcagatccc cttcaaatac ctgcagttca taaacagaaa ctgcctgcca
     2701 gttttccatc gcctggattc cggtaacatt cagacgcaga tattttgctg tcactgcctg
     2761 atccagtgtg atggtatcag ttgtatctgc cggtgcctgt gttctgctgg ctacatcggt
     2821 ccaggtctgt ccgtcatcgg aaatctgaag tgcatatgtt gttgcattct tacgttccca
     2881 gtagatacgg aagctcttca tggtctgtgc ttcattccat tcaagcttta cccaggatgt
     2941 cgcagataca tcatgtgacc atctggtgtt catattgcca tcattgatct ttgcaatttc
     3001 gtttccactc tcagagttgg acgctgttgc cgttgcactc aatgccaggt tggtattctc
     3061 tgctgcattt gctgcaagcg gcgggagctg tacagaagca aggatcatgc tcgctgccat
     3121 ggccatagca ccgatctgtt tcattttttt cttaaacata aacttcctcc tcttcgttat
     3181 gttgttaaat gttgctaaaa acatatttaa ttttaacatg attttttaaa aaagtccaca
     3241 catcttatct aaaacgcata caaaatgcag cacagttaaa aaagataaca aaaaaagccc
     3301 gataaaatcg agcttttcag gatggacctg aggggaatcg aacccctgtc cgaaagccta
     3361 ttcatcaagg catctcccat cacagtcatt attttaacgt tccctcaacg gtgcacctaa
     3421 tgacaggttc acagcctcag tagcttcata agttcttctg ccgccgcaaa gctttggcca
     3481 cagagtgcct cacaggattt gatgccggtt ggggaagctg tgagcaacct cctgccgaca
     3541 agcagccctt aggctgctaa cgcgtaatta tcttctgcgt ttatatttag tttccggttt
     3601 actgtgcggt cccggcccgc agatggcttc cctgactgca aaacccccgt cgaaaccagt
     3661 acaagccctg gtttgtttga agataatctt acagtgcagt ttattttaca cctgaaattc
     3721 atttttgtaa agtgtctttt taaaattttc actttttatt tttctttgca tctttttatc
     3781 tctgtgcgtt ttccatcttt acagattacg caccttaaat tcacgctctg cttctcttcg
     3841 catatctttc tttgcaatat cctgtcgttt gtcgtacagt tttttacctt ttgcaagacc
     3901 gatagagact tttaccagac tgcctttaaa atatactttc aggggaacca gtgtgtatcc
     3961 tttttctgcg atctttcctt cgatcttatt gatctcatag cgatgcatca gcagtttttt
     4021 tacacgcatc ggatctttat taaaaatatt tcccttttca tatgggctga tgtgcatacc
     4081 ataaataaac acttcgccgt tttcaatacg gacaaaggat tcttttacgc tgcattttcc
     4141 cattcgcagg gatttaacct ctgttccgtg aagagaaatc cctgcctcat acgtgtcttc
     4201 gataaaataa tcatgatatg cttttttgtt gtttgcaatc agttttatgg attctgctcc
     4261 catatctttc ctcccgtctc tgacttatcc ccaaatagct gcaggcacaa aatcaattgt
     4321 tgcattttct ttatcagcat caagcacacg tacacggatg ctctgaccga gtttgtaaac
     4381 ctttccaaga tctgagccaa ccatctcatg ggtattttca ttataataat acctgtcatc
     4441 gtaa
//
LOCUS       DAWDWH010000177         4415 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_7766_length_4415_cov_9.839450, whole genome shotgun sequence.
ACCESSION   DAWDWH010000177 DAWDWH010000000
VERSION     DAWDWH010000177.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7766_length_4415_cov_9.839450"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    621..630
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2582..2591
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1037 a         1182 c          836 g         1340 t
ORIGIN      
        1 ttcttcttaa aatatcatat gcttccgctc ttccgccttc cagctccaca tgcaggatct
       61 gtttggaatg cggtgcactt tcctgttctt caccgtcctc attgaggatc ctctttactg
      121 taacctctac attctgaccg tccggtttca tgatctcaat ctgttcaccg acactgaatt
      181 tatttctctg ctcaatgcga cagtaacctt ctgcatcgac ttccccgacg ataccaagat
      241 aggtatattc tttcacgtaa gtattgttat cgtatatctg cgtgttctca tccggtcttc
      301 cataaaagaa accggtcgtg aactgacggt aggtacagct tgcgatctgt gtgcggtacc
      361 acggcatatg ctcttcataa agttttggat cttccagata atcatcgatc gctttgcgat
      421 acgttcttgc aaccgttgcc acatagaggg ccgttttcat tctgccttcg atcttaaagc
      481 tgtcaatccc cgcatcaagc agatccggga tatgttcgat catgcaaaga tccttggaat
      541 taaaaatata ggtgcctctt tcattttcat aaaccggaag atactcaccc ggtcgtttct
      601 cttccacaac tgcatatttc nnnnnnnnnn atttccagcg acacggatgc gtacaggctc
      661 cctggttggc atctctgcct gtaaaataac tgctgagcag acatcttccg gaataagaaa
      721 tgcacatagc tccatgcaca aaggtctcaa tctccagatc atccggaatg tttgcacgga
      781 tctctttgat ctcctgcatg gaaagctccc ttgcagaaac cactcgcttc gctcccagtg
      841 tatgccagaa atgataggtg gcatagtttg tgttattcgc ctgggtactg atatggcgtt
      901 cgatttccgg acagatacgt tttgcgatct caaacacccc cggatcggca atgatcagtg
      961 cgtccggttt cacttctttt aattctctga aatattcctc aacgccctcc agatcccggt
     1021 tgtgtgccag gatattggct gtcacgtaaa ctttcacgcc atgctcatgg gcaaaggcaa
     1081 tgccctcttt catatcttcc atcgaaaaat tcttggcttt tgcacgcagt ccaaatgctt
     1141 cgccgccaat gtaaacggca tccgctccaa agatgaccgc cgtctttaat acttccaggc
     1201 tgcttgccgg caccagcaat tctggtttcc tcatcgttct tcctcccatt tatctattcc
     1261 tttattttca cgctgagtgt tgctccatcc ccgatcggca caatgctggt catcagatgc
     1321 tcattgtgtt tcagttcgta gagatactcc cgcatccgtt tgtatattgt cctgtttctt
     1381 cgctctactg cataatggga ctcgataatg tcaccatctt gcagcacatt atccgagatc
     1441 aggatgccgc cgactttcag tagacgcatc acatccggca gaaaatgaat atactgccct
     1501 tttgcagcgt ccataaagat aaaatcataa gaaccctcaa gttctgcaag aatctctgtg
     1561 gcatcccctt ccagaaaacg gatctgtgac tccttcccgg cttttctgaa attttctttt
     1621 gcgatcggaa tgcgtttttc atacttttca attgttgtga ttttggcctc ttttggtgca
     1681 tattcacaga acagcagtgt ggaaaatcca actgctgtcc ctacttccaa tatattgacc
     1741 ggacgctgca tctgcagcag caccttgaga aagctctgca tttctcttcg tatgatcggc
     1801 acatgatctt ccagtgcttt tacctcaagc tccgtcagga acggggtatt tcccgtatcc
     1861 agggagttga taaaagctgt cattctttca tctactatca tatcgttact ccgtctcagt
     1921 ctctttttca tccgcccggc tcagtactct cagcatctct tctgctgtct gtgaggtgtt
     1981 cagtacatag cttccgggct caatttctcc tttatattca gaaagcagat attgaatgcg
     2041 aaaaacattc ttatctttga tcagtttctt ctcttccaga agctttgcga tttcaccaaa
     2101 ggacatatct ttggtgatcg taactgctac cttttttccg ggagcctcac tcatcgcttc
     2161 ctgtgcaaat atctgatatc cgaatgtgta ggcccgtctg ccggtaaaaa tcagaacaag
     2221 cacaagaact gccagcaata cgattttcac ggcaatgccg actgcaattg attttgcact
     2281 gttctctttc atgcaccctc tcctgtctta ccagtacatt aatcctgcaa ggaacagtac
     2341 tagtccgttt ctatgatatc tgcaaaattt tcaatttcca tatccacaga tgtcagtatc
     2401 tcatccgcac atctctgaat gatcctgcat atctgaagct ccgctgccag atactcctgt
     2461 ataaccggat tcttccgcat atcttcgtat tcccgctcca gattctgcac atcatcaaac
     2521 agatcgtaag cattgcttcc attctgaagc agataactct ttttgcggaa ctcatccagc
     2581 cnnnnnnnnn ntgtcgaagc tgcggctctt cggatacttt tctgctcact tcccgaaacg
     2641 ctttgtacgc ttcgcttccg cgaagtgcag ccgaaagctg caccgtatat tcctttattt
     2701 catccatttt ctatcagccc catcatactt ccataatgat cggcaggatc atcgggcttc
     2761 tctttgttct cttccagagg aactcgctta agctgtcacg gattgctgtc tttaaacgtc
     2821 cccagtcggt cactcttcga tccatgcagt cctcaatagc ttcttctact acatgttttg
     2881 cttcatccat cagtccttcg gattctctga cgtatacaaa tcctctggat acaatatccg
     2941 gacctgcaag aacttgattg ctgtattttt caagcgtcag taccacgatc agaataccgt
     3001 cctccgccag atgctgtctg tcgcgaagaa cgatatttcc gacatcacca acacccagtc
     3061 cgtctaccag aatcgctccg gtctgtacac ggtctacaac ctgtgctttt tcttcatcta
     3121 actccagaac atctcctgag gacaacagga aaatattgtc ctttggaata ccaagactct
     3181 ccgcaacctg tgcctgtgct ttcagatgac ggtattcacc atgcaccgga atcgcatatt
     3241 tcggatgaac cagagaatag atcagcttga tctcttcctg acatgcatgg ccggatacgt
     3301 gggcatcctg gaaaataaca tccgcccctt tcatcgaaag ctcattgatc actcgggaaa
     3361 ctgctttttc atttcccgga atcgggttgg agctgaaaat aacggtatcg cccggcttga
     3421 tctgcacttt acgatgcatg ttggatgcca tacgggaaag tgccgccatg gactcgccct
     3481 ggcttccggt tgtgatcagc accatttgct catccggata attcttcatc tgatccaact
     3541 cgatcagggt attctccggc acgttcagat agccaagctc tgccgctgtg ctgataacat
     3601 tgaccatact gcgaccctct accacacatt tgcgtccata tttataggca gaattgatga
     3661 tctgctgtac acggtccaca ttggatgcaa acgttgcaat gatgatcctt gtatttttat
     3721 gctctgcgaa aatattatca aacgtcttac caacggtacg ctcggacatc gtaaatcctt
     3781 ttcgctctgc attagtacta tcgcacatca gtgccagcac accctttttg ccgatttctc
     3841 caaaacgctg cagatcgatc gcatctccga ataccggagt gtaatccact ttaaagtcac
     3901 cggtgtgcac cacgatacct gccggtgaat aaattgcaag tgcagaagca tctgcaatac
     3961 tgtggtttgt cttgataaac tcaatgcgga attttccaag attgatcgac tgtccgtgtt
     4021 ttacgacttt tctctttacg gacttcagaa gattatgctc tttgagctta tgctcgatca
     4081 gtgcgatcgt aagtcttgtc gcatagaccg gaacattcag ttcttttaat acatatggca
     4141 atgctccgat atgatcctca tgaccatgcg taatgacaaa accttttact ttatgtgcat
     4201 tgtttttcag gtatgtgata tccggtatca caaggtcgat ccccagcata tcgtcctcag
     4261 gaaaggacag cccacagtca accacaacaa tactgtcttc gaattcgaag gcagtaatgt
     4321 tcatgccgat ctgttcctga ccgcccagag gaatgatcct cccgttcgca gtgttcatat
     4381 gtttcacaaa tttttacctc ctgtgtctgt gccgg
//
LOCUS       DAWDWH010000178         4397 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_7792_length_4397_cov_14.005297, whole genome shotgun sequence.
ACCESSION   DAWDWH010000178 DAWDWH010000000
VERSION     DAWDWH010000178.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7792_length_4397_cov_14.005297"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3746..3755
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1501 a          861 c         1115 g          910 t
ORIGIN      
        1 agcagcttgc acagtcggag aaaaatcttg ctttatggca ggcaaaacag aaagatttcg
       61 gcttgctgga aacggaattt tccagagcag aacaaacgga gaaagaatac aaaacacaaa
      121 agcagacgac agaaaaaaga cgtcttgcct atcgggcggc cagcctggaa tttgaagaaa
      181 aaaatgccgt ttacgagcag aaacgaaaga tctttttaaa tgctcaggca ggttttctgg
      241 cacaggagtt aaaggagggg tgcccgtgtc cggtctgcgg ttccacagag catccttctc
      301 cgtgtaagct tgaaaaagaa caccagaatc tgacaaaaga gatgatcgag gagctggccc
      361 ttcaggcaga acagctgcgg ggaaaacagg aacaggcagc agcggcggca gaagccgcag
      421 ggagccttct ggaagaaaaa cagaaaaatg ccggggaaag ttttgaacat ctccgcagca
      481 gggcaaaaga agttctggat ttagaagaaa tatgtgcaga ctttctggag cagatgaaag
      541 gtgaattttt acaccagagt aaaaaaatgg aagcagaagg cagacagctt caaaaagaag
      601 tgaacgagta tcagatgctt ctttctgccc ttacaaaagc agaaaagcag aaagagcaat
      661 ttcaggagaa gcttgagcag tccgcaaaag aaacagcaga tgccaaaacc gctctggagc
      721 gaagccgtgt gatgctggag agcctggaag gatcaaaaga ttaccagaat cagcaggaag
      781 cacaggcggc gtatgcaaaa gcagataccg ccaaaaagga aaaagatgta cttgtcatgc
      841 gggcagagga gagactgcaa aaagcacagg aacaggaaaa ccgggcacag actttaattg
      901 caaagtataa aaaagaaatc cctcagcaga tggaagagca aaagatccga cagcaggctt
      961 accgggaaat tctggaagaa aaacattttc cggaaacggt ctggatggaa ctgaccggaa
     1021 cttacaccaa aacgcaggta cagaaatgga aacaggatgc acagaaccac ggcagacaga
     1081 aagcggcagc cgaaagcctg ataaattctg ctagggtagc gatcggcgaa cagccaaaac
     1141 cagtcctgga agaactggaa aaaaccagtg aagcagcaga agcggcgtgg aaagaaagtc
     1201 agcgtgttct ggaacaatgg caggaaatct ggcggatcga tcagaaagtc tgccaggcat
     1261 taaaaccgca gatggaagaa cgtagtgaag tgatgcatca gtatgaaaaa ataaacggac
     1321 tgtatcagct tctgtccgga aacaggaaaa actccagaat ggatatcgaa acatatgtac
     1381 aaaggcatta tctggagcag atcctggaag cggcaaacca gagatttcag gacatgtccg
     1441 caggacagtt tgaactgcgg atgtgcgata ttgacaaagc cggaacaggc aaaaacagag
     1501 gtctggatct gatggtttat tctgctgtga ccggaaaagt ccgtgaagtg cgaacgctct
     1561 ccggtggcga gtcctttatg gcggcacttt ccctggcact tggcatggca gatcagatcc
     1621 aggaaagttc cgcagcgatc aaccttgata tgatgttcat cgacgaaggc tttggatcac
     1681 tggatgaaca ttccagagat caggcagtaa aagtcctcca gaacatggca gaaggcgaga
     1741 aactgatcgg tattatttcg catgtgacgg aactgaagca ggagatcgaa gatcagctga
     1801 tcgtgaccaa agacgatcag ggaagccata cccgctggca gatcagttaa atcggatgca
     1861 ggtatcaaaa gatatcagag gtactctcgt atggcgtctt ttgacccaaa catcatgata
     1921 tgagtatgga gaaaggaggg gacaggacat acaaaacaaa aagaaaaaga cagaagtgat
     1981 cgttgttgac aaacgcccga caatggctga atggccggtt cgaatctggg cgatggaaga
     2041 aataccggaa atttttgatc tggaagcaag aaagtccatg aaaggaactt ttaaccagta
     2101 tcacatggta tattcgccaa ttcgccgcac ggcaccggat tcttttgaat atatgttcgg
     2161 gtacggcgaa ggggaaattt tttatctcaa aaatgaaaaa aataaagttc gcaggaccgt
     2221 tctgaaatgt agtcagatag aggagattta cacacagaga gaattgctca atgcaaagat
     2281 tatcgtgaaa tataaagcag atttacagga cagggaactg gaaacgatgg aattccctta
     2341 cattccatcg gtatattatc tttatgatcc gtttctgaac tggatgcttg gtctggatca
     2401 ggaatttgta cctgcacttg cagagcagga acatccacgt ccgaaaaaac tgtacaaaga
     2461 aagtccggtc atgtacaatt atgtgctggc agcttaccga ctgggagatt gtatcggaga
     2521 ttataaatat acctcagaac agcaccgcca caagtggatg ccgtggaaaa aggtactgga
     2581 agagtggctg gaagttccga tgagcagagg aacctttaca ctgcacagcc tggaatatct
     2641 gaccgagtgc ggttatctgg aactgagaaa taaaaatgca gcggtacagc tcaagaaaca
     2701 gtgaaaagaa ttgtgcagga acctcccgga tgatgtagct tatgcacagg cacatcacgg
     2761 tttcaggctt ttgctcctgg cattatagtt aattataagg aaaaaacaaa tatgaataaa
     2821 aaattagagg cattacagga atatttaaaa gaactcggaa gcgtggcaat cgcattttcg
     2881 agcggtgtgg attccacctt tcttctgaaa gtggcacacg atgtgctggg agatcaggta
     2941 atcgcagtga cggcacagtc ctgctctttt ccgaaacgtg agctggaaga agcgaaagca
     3001 ttctgcaaaa aagaaggcat caaacatatt atctgcgagt cagaagaatt acagatcgaa
     3061 ggattttccc ataatccaaa gaaccgatgt tatctttgca aacatgaact gtttgaaaaa
     3121 atcggaaata ttgcaaagga aaacaatatc aaatatgttg cagaaggttc caatatggat
     3181 gacaacggcg attatcgccc gggactgcag gcagtggcag aacttcatgt aaaaagtccg
     3241 cttcgccacg tagagctgac aaaacaggag atccgtgatc tttcaaaaga aatgggactt
     3301 ccgacctggg acaaacagtc ttttgcctgt ctttcctcac gtttcgttta cggagaagag
     3361 atcacagaga gcaaactttc catggtagac caggcagaac agctgcttct ggacatgggg
     3421 ttccatcagc tgcgtgtccg catccacgga cagatggcaa ggatcgaagt gatgccgtcc
     3481 gaatttttga aactgatgga agatgcaaac agagaaaaga ttgtttctga attcaagaaa
     3541 atcggtttca gctatgtgac cctggatctt cagggatacc gcatgggaag catgaacgaa
     3601 acgatcaaaa cagattaaaa ttgaccgagc tctttagagc gagagatgct tcttgtccga
     3661 agagcaagaa gatgggcatt aagaaaactt atatgtaaaa tagaacgcca tgatgattcg
     3721 aaaaacaatc tcctgacacg ctgcannnnn nnnnncgtcc tcacagatcg ttggtacaca
     3781 ggctgtattt aacgtgatca tgggtgaacg ttacaagatg gtaaccaaag agacaaaaga
     3841 tgtcctcgcc ggtaaatacg gtgcaaccgc aaaaccgttc aacaaagaag ttcagaagaa
     3901 gtgcatcggc gatgcgaaga tcatcacatg ccgtccggca gaccttctgg agccggaact
     3961 tggcaaactg gagaaagaaa tggctcagtg gaaacagcag gatgaagatg tactgaccta
     4021 cgcactgttc ccacaggtag ctgtagactt cttcaaatac agagaagcac agcagacaaa
     4081 ggtagaccag actgtagcag atactaaaaa cggagcatat ccggtataag ctgaggatct
     4141 gatcaaaaca gtctgtttat gacaaagatt tacaggactc ttccgcagga agcgcgaaag
     4201 cgtggaatgt gggagagttt tttagcgttc aaaaaaatac caaaagtcag aaaaaaatac
     4261 atatactttc aaataaaaag ttgtggcgga ggttttagaa tgtgcaaatg aacgccatag
     4321 aaatgatttt ataaaatata taacgccatg atgattcgaa aacaatctcc tgacacgctg
     4381 cgtttcctgt tcggttc
//
LOCUS       DAWDWH010000179         4359 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_7869_length_4359_cov_11.268355, whole genome shotgun sequence.
ACCESSION   DAWDWH010000179 DAWDWH010000000
VERSION     DAWDWH010000179.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7869_length_4359_cov_11.268355"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1825..1834
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1134 a         1071 c          840 g         1304 t
ORIGIN      
        1 tcatggcgtt atatatttta taaaatcatt tctatggcgt tcatttgcac atttaaaaac
       61 tcgccgtaaa ttgatccagc acttctctgg cttcttccac cttcttttca tcagttctcc
      121 agatttcgaa ttcactcatc gacggcagtc ccatattgat cccttctttt cgataaacca
      181 tacggctgtc cagtgtcatt ttcccagaca tatggaaggt cgtgatcccg gtctttggtg
      241 ccagatagcg aatcgcatct gcatcgattc cggctcctgc catgatatca acttttccgt
      301 ctgctttcag ggcaagctgt tttaagattt ctacgccttc ttttgcactt tttctctgac
      361 cggatgtaag aatggtattt acacccagtt cttttgccgt ctccagagct tcaaacggat
      421 cacggcacac atcaaatgca cgatgcagtg tgatgctcat atctcctgct gcatcgatca
      481 tgcgtttcat cgcttcgaca tccagtgttc catccggctt taaagctccg atcacgaccg
      541 catctgcacc aagctttcgg aaagtacgga tatctccgca taaaatatca atttcatgtc
      601 tggtgtaaca gaaatctcca aaacgcggac gcatcagtgc atggatgcgg atatccatct
      661 gatttttaat ttcctgaaac agggctgtcc ccggagtcac cccgccgatc acaagtccct
      721 gacagagttc cagtctgtct gctccgccgt tttttgctgc gattgcagat tctacgctgt
      781 ctacacagca ctctaaaata cccttcatga cgatttcctc ttttctttca tatctttctg
      841 tgataccggt ttaccatcta ccacgatctt tgcattcgga taaatctgct ccattcgttt
      901 atccgggaaa tgtttgtcta aaaatttttc cagatccgta cggctgtcct ctgccgccgc
      961 tgtcatctgc ggcgttttct gaagactccc ctgctgtctt tgattgtaac ggtttagagc
     1021 cagcgttgac atgccggcat tgaagatcat aaacaggatc agtatccagg tcagaatatt
     1081 tcctgttttt atcggaattt tttcgatcag atttgaaagt ttcggataga tgatcttcag
     1141 ccagactact gcggcaatcc cccagaaaaa gcagaacagc agattgatcc tgccgccaag
     1201 attaaaaggc agcttactgt aatcccagaa aaccgtcccg aaaaccagct cggtaaatat
     1261 gctgcaggca tattcataag ccccgccaag caccgttcca agcataaaaa tataacggtc
     1321 acttttctct ttatatttat aaagcaatgc cgtgaacatc gcacagccaa gtccccatac
     1381 aatactgaat ggaccataga ccacgctgct tctgctcatc catcgcccca tggaataacg
     1441 gcagaaaagc gtctccacaa gatctccgat aaaagccccg atcagaaaca gccagaaaat
     1501 cttgaaaaag cagcagcctt ccgcaaatac cgtcttttct tttttcgtat ttttcctgac
     1561 ggattctttg atattctctg tcttaatatt cggatatgcc cgcatcatac gcttctgtat
     1621 ccggacagtt atggcattgc caacgttttc cgatactttt tgcatatttt cattgagctg
     1681 catgatcctt cgaagtttga tcttcagctg taaaatagcg atcacagaac caaggaaatc
     1741 aatccccatc agaatatacg ctatgatcag gataatcctt ccaataaaat gcggaatctg
     1801 ttttaaaatc tgcaggatca gaggnnnnnn nnnnagagga ttggcaaatt tcatcatcac
     1861 aacgatcgcc gcaccgaaga tcagcagatg ccagattcca aaatatccct cgtactgaaa
     1921 acgattctta ctgtaatccc accatttccg atgaaaaata tgctccagga tcagccctgt
     1981 cataatggac acgaacgctg ccagaagtgc tcctcctata aacaggaaaa aaatccgatg
     2041 ccgcagttcc ggcaggaaaa tacagcacag tacggcacag acaccatacg acaggcagaa
     2101 cggcatgttc ataaaaccag tatttacaaa ttttcggtta cgtacggcat ttgccacaac
     2161 gcctgcacac cagccggcaa aagaataaat cagaaagaac cagcaaagtt cgtaaaatgt
     2221 atagaccaat ttattttccc cttttctctt tttcttatgt gtgttactat ataaagatgc
     2281 cagggtcaaa atgtcgttct tttttataac gaaacaattc acaggcatca ttgatgtcaa
     2341 aagatttttg actctgacag acagtatacc tgtaaacagg attgcttgca agagaaaaac
     2401 cgattcgttt tatttttata taaaaataat ataactgtta ttttaaatat acattttaca
     2461 tttttctatt tctctgtata ctaagggacg gactttgaag cgatgtccct gaatcctgat
     2521 gatcccaaca cagatgcgtg agcatcttta tacccccgaa ccaagaaagg atggcggcag
     2581 cttatgtttg ataagttatt ttttacaaaa aacggtcaaa tatcggaatc actggcttta
     2641 ggaatttttc tgactctggc aggcggtttc caggatgcgt attcttacaa cgtgcgtgat
     2701 cacgtgtttg caaatgctca gactggaaac attgtattgc tcggacagaa tatcgcttcc
     2761 ggaaacctga tgcatgccct gcgttatctt ctgccgcttg ccgctttttt tcttggtgta
     2821 taccttacgg aatggatcaa gcatttttat aaaaccagcg tcaagatcca ttggaggcag
     2881 attgttctga tcatagaaat cctgattctc gggatcaccg gatttctgcc gaatgaacct
     2941 accctcaatg tgatcgcgaa catcatgctt tctttcgcct gtgccatgca ggtcgatacc
     3001 ttccgcaaat tccgcagtat tccatgtgca acaaccatgt gcatcggaaa tatgcgaagt
     3061 gcgaccgaat atctctgcct gtaccatatc agcaaagatc cggcgatgaa atacaaaagc
     3121 atgcactgtg gatttattat tctggttttt gcgatcggtg ccgctttcgg tgcctcctgc
     3181 tccgcctggt ttggcgtaaa ggcgatctgg attgccatgg ccatgcaggt agtcggattc
     3241 tttatgatgt ttgcacatca ggatatagaa gcattcgaga aattcgaaga agaagaaaaa
     3301 gaaaagattc gtcagacaca cgccagaaaa aaataaacat gaaactatgt ttattgctta
     3361 cctacatata tgtttactac ttactcacac gaaaaaagga actgccgact gcagttcctt
     3421 ttttcgtttt caaactttct atcttcccgg tattttattt ttcgtgaggc agactcagct
     3481 catcgatctt cttccgttct tcttctgtga aatctgcact ttccagaact ttcagattat
     3541 caaggatctg tgttgtcttg gatgcaccga tcagcacgct ggtcactaca tggtctttta
     3601 atacccattt cagtgccatc tgtgccaggc tctcacctct ctgttttgcc agttcgttca
     3661 gtccacggat acttgccagt ttttcttccg tcagctgatc tgcttttaag aatcttccgt
     3721 ccgttgcaat acggctgtca gccgggatac cgttcaggta acggtctgtc agagttccct
     3781 gtgccagagg gctgaatgcg atgatccctt tctttgcttc tgctgctgcc tgtttcagtc
     3841 cgttcttctc gatcgtacgg tcaaagatgg aataacggtt ctggttgatg atatacgggc
     3901 agtgcaggtc tttcagaatt gcttctgcct gcttcatatg ctctccgtca taattggaaa
     3961 tgcctgcata aagtgctttt ccgctgcgga cgatctgtgc cagtgtctcc atggtctcct
     4021 caagcggtgt ctccggatcc atgcgatgat gatagaagat atcaacgtat tccagcccca
     4081 ttcttttaag gctctgatcc agacttgcga tcagatactt tttgcttccc cagtttccgt
     4141 aagggccatc ccacatcaca taaccggctt ttgtacttac caggatctca tcacggtaag
     4201 caccgagatc ttcttttaaa attcttccga aattgcgttc tgcacttccc ggttccggtc
     4261 cgtaattgtt tgcaaggtca aaatgtgtga ttccatggtc aaatgctgca aattctactc
     4321 gaacggtacg cctattctga aattagcggt acatccatt
//
LOCUS       DAWDWH010000180         4358 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_7872_length_4358_cov_9.195213, whole genome shotgun sequence.
ACCESSION   DAWDWH010000180 DAWDWH010000000
VERSION     DAWDWH010000180.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7872_length_4358_cov_9.195213"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1062 a         1056 c          859 g         1381 t
ORIGIN      
        1 cgctttttca gattcctttt ccaggatcac tttcggtgtc ccgctgctta cttccgagca
       61 ggacagccgt gaagtcgtca aggttctgac tgatgctttt ggcggcatcc caaaagacga
      121 ggcacttgtt ttcatgggac atggaacacc gcattatgcc aatgcgatct atgcagcact
      181 ggactacacc tttaaagatc ttggttacca caatcttttt atgggaactg tagaagctta
      241 cccgtccatg gactccctgc tgcgtctgat cggcagtctg aagccgtccc gcgtccatct
      301 ggctccgttt atgatcgttg ccggtgacca tgcacacaat gacctggcag gagacgatgc
      361 tgattcctgg aaatcccagt ttgagcaggc cggttatccg gttctctgtc atttaaaagg
      421 tcttggtgaa tacgaaggaa tccgccaaat tttcctgcgg catatccgcg aagcacagta
      481 aaggagtttt ttatgcatat gcatcatcat aacaccggag gtattgctat tgattcttat
      541 gcctgtaatt ccaggcttcg gcatatcagt gcgggattca aagctggatt ttccatgctt
      601 ctgcttctgt tctgtatttt tgcagaccat cctgttatct ctgttttgtt gatcttattt
      661 gctgcttccg tcacggtttt tcttggcggt attcctttgc atgattatct ttccgtgctc
      721 gggattcccg ttttttttgt gctgcttggc ggcatcgcca tcgctgtcga tttctcttta
      781 cagccgactg gcgattttta cctgaatctg tttttttgtt acctttgtgt cacgtatgcc
      841 ggcattctga aagcgttttt tctgatgctg aaagtgttcg gtgccgtaag ctgcatgttc
      901 ctaatgacac tttccacacc gtcacacgaa cttttctcgg ttctccataa gattcactgc
      961 ccttctcttt ttacagaact gatgcatatg atctaccgct acatttttat tctgctggag
     1021 gtacaacata atatgcagat ttccgcggaa gcaaggcttg gattctgcga tttcagaacc
     1081 tcctgttatt ctttcgggca gattgcagcc aatcttttta tcgttgcact gaaaaaagcg
     1141 aatgcctatt atgatgcaat ggaagcacgc tgctatgatg gaacactttg ttttctggaa
     1201 gagaaaaaga aactgcccct tccgtacatt atagcttccg gaaccttttt tctgtttctt
     1261 gttgttcttt ggcttttcat aagatgaatt taagatagga gatagtatga aagaatccat
     1321 tcttgaagta aaaaatctgc attttgcgta tgacgaacac catacggttt tagatgggat
     1381 cagtctttct gtctttgcag gtgagcgtat cgccatactt ggttccaacg gagccggaaa
     1441 atctacattt tttctgaacc tgaacggagt gctcactcca gccgaaggcg aaatttcata
     1501 ccgcggtaca aaaatcacca gaaaaacact gaaaaaactg cgtcagcata tcggtattgt
     1561 ctttcaggat accgataatc agatcattgc ttcttccgtt ctcgctgaag tctcttttgg
     1621 tcctatgaac ctgaagctgc ctcgttcgga agtcgaagaa cgtgtaaata ctgcgttaaa
     1681 ttatatgaac atctcacaat ttaaagaccg ccctccccat tatctgagcg gaggtgagaa
     1741 aaaacgggta tcgattgcag acattattgc catgaaatcc gaaattatta tttttgatga
     1801 accaactgca tctttagatc ctctgaatgc tgccatgctt gaagaagtcc ttgaaaagtt
     1861 aagccgagaa ggaaagacac ttctgctctc cacgcacgac gtggattttg cctaccgctt
     1921 tgcagaccgt attcttgttt ttgacaacgg ccgcatactt gccgacggca cgccgcttga
     1981 agtttttaag aatatttctg tcttaaaaca ggctaatctg aaacgcccgc ttttgctgga
     2041 tgtctgcgaa gatctggctg cacatggact ttttccgaaa ggatcttttt atcccaggga
     2101 tttttcagaa tttcatacat atcttgcaca gagaaattct ttttaataaa aactgccgca
     2161 cagtttgaat ccgtacggca gttttgtttt cttatttttt ttcttcctct ttatcttccc
     2221 attttttccg ttcataaaat cttcccatca gaaatgcgat cacaccaact ccgatcccgg
     2281 tctgtacaca gaagatcaga ctttcaattt ctccaggcag ttcacttcct gtaagacttt
     2341 ctataaccgg agtaaaccat ggttcataag cttctccttt gatttcttct accataacac
     2401 tgcccgcatc atcggaaccg ccaaattctg ctcctttttt tgcaaagaac ggaacaactg
     2461 cgagcagcac agctgcaact aacagaagaa ggattgtttt tttattcttc gcactcattc
     2521 tttttcctcc tcgatctcaa aatcaatcgc ttttagttca ctttttgcaa aactctccag
     2581 tgcgatcaca acaacaacgg taagaattcc ttcgatcact gccagcggaa gctgtgtcgg
     2641 tgcaaatata ccgagaaatt ttacagcaga tgctgttacc ccaccctgtg cagacggata
     2701 ggcaagtgca agctgcacac tggttacgca ataggtaaac aaatctccaa gtgatgcggc
     2761 aagaaaaacc gtgatacggc ggttgatcct ggtttttgaa agtcccttat aaatcagcca
     2821 ggacaggatc ggacctgtaa tcgccatgga aaaacagttt gctccaagtg ttgtcagtcc
     2881 gccatgggcg agcaacactg cctggaagat cagaacaatg attccaagta cactcactgc
     2941 acatggtcca aacaggattg caccaagacc ggttcctgtc atatgggaac aacttccggt
     3001 cacagaaggg attttcaggg atgacagcac aaatacaaaa gctcctgcca ttgcgatcag
     3061 tgtaatggat ctgcggtttt ccttcactct cctgttcagc gatcgaaatc caaatactaa
     3121 aaacggcaga cagacaatgc cccatgcaat acaaaatgcc ggaggcagat atccttccat
     3181 aatatgcatg gcatttgctg tcggtgccac agccagaata gctgccgctg cataaacagc
     3241 ctttgcaagc cacgatatct tctgattttt cattttttct gtgttatttt ttctttgctt
     3301 cttcattttg ttttcctgtt ccttttcttt tttcttaaaa aacgtctgca aatgactctg
     3361 tcacatatta taaagcagtg cattacagat gcaggctgcg atgttgctgc ctcctttgcg
     3421 tcctcttgct acaatatacg gcacatctgc ctgcatgatc agttcttttg actgtacgac
     3481 gttgacaaag ccgaccggaa caccgatcac aagttctggt tttaactttt tctcttcgat
     3541 cagctcataa agccggatca gggcagtcgg tgcattgccg attgcaaaaa tcagcggtct
     3601 gttcatggct gccgccttgt ccatacaggc agttgctctt gtcgtcccgt ttttctttgc
     3661 tgtctctgcc acatcttcat ctgacataaa gcagaagact tctccgccgt atgctgccag
     3721 ttttttctta ttgatcccgg attttgccat ctgcgtatcg gtcacaatgc aggctccgtt
     3781 tcggatcgct tcttttgctt tttcgacaac accttcggaa aaacagagat tatctgcata
     3841 atcaaaatct gcactggtgt gaatgcagcg tttgatgatc ggttcttgtt cttccggaaa
     3901 ggttctctcc ccaagttctt ctgtgatcag ttcaaaactg cgtttttcaa tttcccgcgg
     3961 aagtacattt tccagttcaa tcttcatcat ctgtcccctc ttccataatt tcataaatac
     4021 gcgaaatatc catatgtttg cgaagtgcat ctgcaagaat attgtactgg atttctttga
     4081 attttgcaaa atccacgcct gcgatctgtg tcatatccag tcctttggct tctccgatgg
     4141 ctgttacgat cgcttttact acttcttctt tttcaaaaat tccatgtaca tatgttccat
     4201 aaacatttcc atgacagacg ccatctgctt ttgccgcacc tttctcttct ccggcaaagc
     4261 ttgtgatttc tgtaaaagca gctgtctgtt ctcccaatgt cgtgctgccc atatggattt
     4321 cataaccttc catttccata tcacttagat tttgcaat
//
LOCUS       DAWDWH010000181         4304 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_7974_length_4304_cov_11.817133, whole genome shotgun sequence.
ACCESSION   DAWDWH010000181 DAWDWH010000000
VERSION     DAWDWH010000181.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7974_length_4304_cov_11.817133"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1301 a          853 c         1137 g         1013 t
ORIGIN      
        1 atgtcatagt caatctgatc acggatctct tataccactg cctggatccg agaatccgtc
       61 tgggggtgaa tcaaaatgga taaatcagca cgaagaaaac cgaagaagca gaaaaatcat
      121 ctgaaaaagc gattttttat ttttgccacg ctggcactgc ttctggttgt cggttcttat
      181 ttcagcgaac acttatgccc atatgatcct tatgagcaga atcttagtat cgcaaaagca
      241 agtccgtcac tggcacatcc gttcggaaca gatcgttatg ggagggatat gttatccaga
      301 gtcatcgtcg gaagcaagac aagtatctat tcgactttgc ttctggttgt cgggatcacg
      361 gtgatcggaa cgatcgtcgg gatcatctgt ggatggaacg gaaagaaact ggatacgatt
      421 ctgatgcgga tatccgacat tttccttgca tttcccggtc tggtttttgc cctggcagtt
      481 gcagccgtga tgggaggcgg cgtacagaat gcggttatag cccttggggc gatcagctgg
      541 ccgaaatatg caaggattgc cagaagtcag acattggcac agaaagaaag cctctatatg
      601 cgggcggcaa tcttatctgg atgcagtaca ggaaaactga ttttgaaaca tattctgccg
      661 aatatcacag gaccgatcct tgtgactgcc atgctggata tcggaacgat gatgatggag
      721 cttgcaggcc tgtcgtttct gggacttggt gcaaaaccgc cggttgcgga gtggggaagt
      781 atgatgagtg ataccagaaa tctgctgacg acacatcctt gggtaacctt tgcaccggga
      841 attgccattt ttctttcagt catggttttc aacctgcttg gcgatacggt aagggatctg
      901 ttggatccaa gaaatgagag gtagatatgt caggaatcat ttgctatgaa aacgtcgata
      961 tctgttacaa tggcagtccg gtaatcaaaa aggtcagctt ttcggcagaa aaaggtgaaa
     1021 tcttagggat cgcaggggag agcggaagcg gcaaatccac attgattaag gcagcaatcg
     1081 gattgcttgg aaagaatgga agcgtcacag gcggaaagat ctggtacgaa gaaaaaaatc
     1141 ttctggattt aaatgcaaaa gaactgcgta aaatctgcgg gagcgaaatt ggcatgatct
     1201 tccagaatgc cggaagttct ttctgcccga tccgaaccgt cggggagcag ctgctggaag
     1261 aaatgaaaga gcaccgaaaa ataaaaaaag ccgaatttga ggatgaagca gcggaaatgc
     1321 ttgcaaaatt cggatttcag gatccaaaaa gaatcctgaa aagttatccg tttgagctgt
     1381 cgggtggcat gcagcagcgt gttggcgttg cagcagcgat gctcttaaaa ccgaagatcc
     1441 tgcttgcgga tgagccgact tcggcactgg atgtttcgat tcagaaacag gtcgtagagc
     1501 agatgaaaat gatccgagaa cagtatggga caacgatcct tatcgttacg cacaacatcg
     1561 gagtgatccg tgcgatggcg gactccatgc tggttctgaa agatggaaaa atggaagaat
     1621 acggaaagac gcaggaggta ctgagtcatc cgcagtcgga ttatacaaag cgattacttg
     1681 cagcgattcc tgttcttcgg aggtaaaata tggagccatt attacagatt tcgcatctga
     1741 ctaaaatttt ttcagatgga aaagaggatg aatataaggc ggtggatgat gtcagttttg
     1801 aattgtttcc gggagaaaag ctggcgatca tcggggagag cggaagtgga aagacgacgg
     1861 ttgtaaatat gatcaccagg attcttgaca gtaccgatgg aaagattctg ctcgatggag
     1921 aagacatcac ccactataaa agaaacaaaa tgaaaaacat ttaccggaaa atgcagatgg
     1981 tttttcagac gccgaccgaa agttttgatc cgagatgccg ccttggagat gctgtggcag
     2041 aaagcctgag aaatgccggt atgccaaaaa aagaagctat ggaaattgta aaagagcttt
     2101 ttaagcagtg cggactgccg gaagatttta tgaaacgtta tccacatgaa gtcagtggcg
     2161 ggcagtgtca gagagcagcg attgcaagag cgattgcaat caagcccaga ctgctgattt
     2221 tagatgaagc gacgtccgca cttgatgtca ccgtccaggc agagattctg cagcttttag
     2281 atgctctgca ggaagaacgt cagatgtcgt atctgtttat ctgccatgat attgcactgg
     2341 tgcagaattt ctgtgaccgt gtgctggtca tgtatcaggg aaagatcgta gaacagggga
     2401 aaacagagga agtgctgcat tttccgaaac acgaatacac gcgtatgttg ctggacagtg
     2461 tgctgtgatg ccggcagctg tttgtatagc aaatatacga atcagataga tgtcagcaaa
     2521 acgaatcaga atcgaatgac aagttttata tacatccgat tgaaaaaatt ccaaagttat
     2581 tttgtgacgg agcgatttat ggataccata gtatgtcaga gataagttgt ataatatcaa
     2641 aaagaaaaag agagaggaaa aacgtatgaa aaagaacatg aaaaaaataa tggcattgct
     2701 tcttaccgtg acactcagcc tgggactgta cggctgcgga agcaagaatg acaaaacgga
     2761 aagcgggaca agtacggcag ctgagtcgga tacatctgcg gcagccacag acggttctga
     2821 gaaaaaaacg ctggtgatcg gggatacaac tttcaatcct tccaatgaag aactggatgt
     2881 caatccacac aattcttact ccggatgggc atgcatccgc tatggaatcg gagagacgct
     2941 ggtaaaatat tcagacgata tggagctgga gccgtggctg gcaaaagagt gggaaaatac
     3001 cgatccgctc acctggaaga tcacactgca ggatcatgtg actttttcaa gtggcagagc
     3061 tatggatgcc gaggcagtga aacaatgtct ggagcatctg ctggcaaacc atgaccgtgc
     3121 ggcagccgat acaaagattg attcgatgga agcagacggt caggtgctta cgatccacac
     3181 atccgagcca aagccggcac ttttgaatta ccttggagat ccatatggct gtatcatcga
     3241 tgtggatgca ggatttgacg atggaatcgt agccggaaca ggtccataca ttgcaaccga
     3301 ctgtcagtcc ggcgatcatc tgactctggt gaaaaatgaa aactactgga atggaacgcc
     3361 acatattgat gaactgacga tcaagaccat caccgatgga aatacccttg caaatgcact
     3421 gctctcagga gaggtacagg cagcttatgg tatggcatat gaaagctatc ctatgttcga
     3481 aaacgataac tttacctttt cacagatttc cacatccaga tgtttcttcc tgaaaatgaa
     3541 tttcaacgaa ggttccgtct gtactgatcc ggccgtgcga aaagcgatcg ccatgggtat
     3601 cgacaaagaa ggatttgtgg aaaatctgct ggaaggaaac ggctatccgg caaatggaac
     3661 cttccctgct ggttctgctt ttggcggaga caaagtaacg acagagacgt atgatgcaga
     3721 aggtgcaaag aaagttctcg aagaagccgg atggacggac acagacggag acggtatccg
     3781 tgaaaaagac ggacagaagc ttcagatcaa atggcttacc tatccaagca gacaggagct
     3841 tccgcttctg gcagaatccg cacaggctac gttaaaagat atcggaatgg atgtggatat
     3901 caactgtacg gctgacaaca acagtgttgt gcaggatccg gcagcgtggg atgtctcagc
     3961 catggcaaat gtgcaggctc cgaccggaga tccggaatac tggtttaccg tgtttgcaac
     4021 ttccgatgca acgaaaaatc agggagctta taaaaatgag aaactggatc agttggaaga
     4081 gcagctcagt caggaatttg acaccgacaa acgtgccaag cttgcagttc agatgcagca
     4141 gacggttctg gacgacaatg cctttgtata ctgctctttc ctgaagatga gccagatctc
     4201 acgggcaaat gtaaccggat atatggcaca tgcctgtgat tactatcagg tgacagcgga
     4261 tttggatatt aactgaatat ttttcatatc ctgaattaaa aaat
//
LOCUS       DAWDWH010000182         4294 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_7996_length_4294_cov_10.948809, whole genome shotgun sequence.
ACCESSION   DAWDWH010000182 DAWDWH010000000
VERSION     DAWDWH010000182.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7996_length_4294_cov_10.948809"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3816..3825
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          997 a         1067 c          782 g         1438 t
ORIGIN      
        1 aaacggaatc tctcccgttg gaatcccgtc tacataccaa tccggtttgt tcacaagctc
       61 tgttccgtct ggaacaaaga ctacatcagt caatacttct ccagcttcaa gttcttcatc
      121 gtatacaacc ttcgggcttt catatgccag taccgggatt gcggtagctg cggacatcag
      181 cagcaacgct gccagcaaga ttcctttttt cgttttcggt tttcttttga tcattttgat
      241 tctctcctcc atattatgat aacggatctt tcctctgaaa ttcagcacac cggtattgga
      301 atcctgcact gctgcatcca gtatccgttt tccatattcg atacattctt ctcttgtcag
      361 tgactgacat acttgctcat cgcaatgcaa ttcaatgcac tgacgcactt cctgataaaa
      421 aaggtaagcc agtgggttcc agaaatggac tgcatttacc agaaaggcaa gatgtgacca
      481 gaaggtatgg tgatacctga tgtgtgtcat ttcatgtctg gtctgatact gtgttgcttt
      541 ttcatcttcg gactgtgcga tcacaacggt cggacgcctg atcccctgcg taaaagaacc
      601 gcatttttcc gggacatatt ttatggttac atgatacttc ctggcaatcc ggctgctggc
      661 atcatccaga ttattctttg gcttctgccc cggaatccgg tgtgccagtc tgcgacattc
      721 ccgataagtt ttcagaaaat gcagcatata gatcagaaaa acaacaattc ctgttatcaa
      781 aatatttcgt ttccaggttg ccatttttcc aaatgtcata tacccgtcat ttcctatata
      841 aaatgttttt gaaacatctt cccatcgcac tcctgtattt actttccgaa agagaatatc
      901 tttgtcagat aagtctctta tcattctctt taacggctct gacagcagcg gaaacggaag
      961 cagataaaat gccatcacaa gtcgcagcag aatccagcgg ccggatgcac tcagtttttc
     1021 ttttcctgtc atccggtata gcaatttata cgcaacatat gctacgcttc cgaccagtcc
     1081 catatatatc ggcaaatccc acatatttct tctcctttgc tcccttattt tcgctttatt
     1141 cttctttttc gtctaaaatt ttgcggagct catctgcctc tgttctggtc agtgattttc
     1201 ttctggtgaa tgccagcaga aagttcttca gggatccctg gtattcattc tctaccagat
     1261 tttccgtcca ctgtccgatc agctcatctt ctgtgatcgc cggataatat aagcgataat
     1321 ttcctttctt gatcgctgtg atcgcacctt tatctatcag gcggttcaga agcgtataga
     1381 ccgtctgctg cttccacgta tagcctctgt tctccagcca ggctaccacc tctcttaatg
     1441 ttacctcttc atgtttccag atgtacttca tgatttcatg ttcagaatcg gataatccat
     1501 accaacccat tgtttttacc tcctgttata ttatattctt atagttcagg agccggtggg
     1561 attcaggcag ctcctattac tatatcgtaa tataacttta gcaaaaacag gtcctccggt
     1621 caagtactat ttctcttttt cttgaccttt ggacctgata ttttttacat ttcctgcttt
     1681 tccagcacac ggcaggagat cgtccaggat atgaaggtaa gcaatgcgac caaaaggatt
     1741 ccggcaatgc caaacaaaat tttataactg ctgattatct gccataaatt tctgccccag
     1801 ctactgattc cctgcatgta ctgatccaga tttttcataa tttcaaagcc cagaaccaga
     1861 ataccaaccg ttgccagaat cacgatcttt gatttttctg ccccgaattt gatctgtaat
     1921 ggaaccagca gatctgcaag gagaagcaca atcagcagac atatcattgt ctctacgcca
     1981 aggacacctg ctgtcatttc gctttttcta accagcagaa gaatcccaca ggcgatagct
     2041 acgatcagtg cggaaactgc tccgaatccg catacaaaga aatatttttc tgttgcatag
     2101 tcttttcttc tgaccggaag agtaaagaga aagctgtttc cattgtccat gctgtcataa
     2161 ctgatcgtgc tgatcgcaac gctcgtcatc acggatgcca tataaggcaa gataaaggaa
     2221 atctccacac ctgacagaag cagtaaaact cccattccag caaagatcag catcattttt
     2281 ttctgatttt ttattattct gatatctttt tcgaacaatc ctctcataac gatactcctt
     2341 tccggctgct gccttttaac atggttttca tcacttcatc gatcgttccc ttttcaatca
     2401 cgatctgcgg catattttcc atgtaatatt gtttctgatc ggtaagacag cgatatccaa
     2461 aatcttcttt gcagtatgcc agaatatatt gtttatccag tgccgcaaat tcggattcgg
     2521 tcacttttaa aataccgtat tgatccagca gaacatcggt atcttcgtgc agacggattt
     2581 ttccctgatc gatcagataa agatcatcgc agagattttc cagatccccg gaaatatgcg
     2641 aactgatcag aattgcccgc tcttcctgct ccatataggc acgcagagca tccagaatcc
     2701 cctctcttgc catcacatcc agaccagagg tcggctcatc tagtatcagg atttctgcct
     2761 gatgagatat tgccatcaga attttcagtt ttgccttcat tccggttgac atttctttta
     2821 tctttttatt catcgggata gcgaatgttc tgcatttctc ttcgaaatct gctttatcaa
     2881 accgatgata catctttttc aggatcgcac ctgcatcttc cgctgtcaga atattcggaa
     2941 aggtcgcatc ggaaagtacc actccgatct tttcccattc ttcgtttgtc agctgctgcg
     3001 gtgtctttcc catcatccgg atctcaccgc cgtctgtatg gatcagtccc agtgatgctt
     3061 tgaaaatggt cgtctttccg gctccgtttg caccgatcag cccggtgata cttccacgct
     3121 ttacctgcat cgaacattcc agtgaaaaat ttgcatattg ttttctgaca ttttttaaat
     3181 ccagcaaaat ctcttccatc ctcttctcct cctaatcttc caggatcagc tcaaacagct
     3241 cccgtatctc tgcttctgtc atgccacagc tccttgcttt gcggatcgcc tgttccagat
     3301 ccgtttctac ttcttttctt ttttcttcca tgcgaagctc ttgattgact ccggatacaa
     3361 agcttccttt tccatgcact gtaacgatga agccctcctg ttccagagca tcgtaagctt
     3421 tttttaccgt caacgcactg accctcagtt ctttcgcgaa ggttctcaca gagggaagca
     3481 tctcctcctg tttcagttca ccgcttaaga ttttattctt gatctgtgtg cagatctgct
     3541 catagattgg ctgcatggaa gaattgttga tgatcagttt catagattcc tccatttctt
     3601 tgcttttgtt gcaaacagta tataacagtt tagtactgtt gtcaactgtt atgcactgtt
     3661 tatattcatt tttttattta catctgtcca agaatccctc tgacattctt gctgcaaggt
     3721 gcacgtcatg catcgcatga catattcttc ttatacaggt atcagaagca caaaaaacgt
     3781 ccacccggag atctactctc cggatggacg tctggnnnnn nnnnntttgc ttttcacagc
     3841 ggaatctttg ccgtaataaa ctttaccttc ggctttgcga tacgtgcgga ctttgaagta
     3901 acaggttcct ttctttttcg gcagtttggt cacgaaagat acggttttgt ttttgttgat
     3961 ggtttttatt tttttgtaag tgcctttttt ctttgtgctg cggtagattt cgtaaccggt
     4021 agccttggat acttttttcc aggtgagtcg gactttgttt tttccggcat ttttgatgga
     4081 tctcagtgtc gttttggccg gtgtatattt tttccggagt gctgcctcct ttgcggcttt
     4141 ctcacgctct gctttttcct tttctgccct gtcacgctct gcttttttct gcggatactc
     4201 tgcaagccag gcggacactc tctgctctgc ctgagcaaac tgttcttttg tgctgtccat
     4261 attggttacg attttcagaa cgtttttgta tata
//
LOCUS       DAWDWH010000183         4290 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_8006_length_4290_cov_8.248642, whole genome shotgun sequence.
ACCESSION   DAWDWH010000183 DAWDWH010000000
VERSION     DAWDWH010000183.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8006_length_4290_cov_8.248642"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2435..2444
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3211..3220
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          961 a         1162 c          811 g         1336 t
ORIGIN      
        1 ctgcaatccg ccggaggcta tatcaagttt tatcagagat aacagatttc atagcgggct
       61 tctcccgcct catctatggt catgatcatg taacttggcc tgcgtccttc ctgtctcgga
      121 taagacagaa tgcccgggtt taagatcgtg atcccgtctt ccgtatccag atacggtttg
      181 tgtgtatgcc cgaacatcac aatgtctttg cctctgctcc ttgcctggcg gcgaagctcc
      241 tcggtattga gggaaacata ataataatga ccatgtgtga tcagcacttt atatttgccg
      301 agttcaaact cgagttctct tgggagcatg gaaaaaaagt cattgtttcc actgaccata
      361 taggctgcac acggaaccag acggcgtatg taatcttcct gtccttccac atctccgaga
      421 tggatcaggg catcgatcgg ttttgtcatt tcgatcaccc gctccagatt ttccagtctt
      481 ccatgcgtat cactgacgat cagtatcttc atatgctccc tcctcgcaat attttctcag
      541 ctgttctttg atcgcacgca gtgccttgcc tctgtggctt aattcatttt tcacatccgg
      601 ctccagctgt gccgttgtac atccatactg tggcagataa aagatcggat catatccaaa
      661 gccattctca ccctgttctt cgtatccgat gattccttcg atcgtgcctt cactgtgcag
      721 cacttctcca tttggaagaa ccgctgcaat cgcacagaca aaacgtgcgg tacgtttctc
      781 atccggcacc ccttccagac ggctgatcag atttacattt ttgatatgat aggaagtatc
      841 ttctcccata tagcgtgcag aataaatgcc cggttccttg ttcaggtagt caatctccag
      901 tccagaatca tctgcaagca cgatatcatg gcatttttca aagattgcac ttgccttgat
      961 ctgtgcattc tctgcaaaac tcgttccatc ttctacaatg tctgcctcta taccggcttc
     1021 tttcatcgag acaaccggca tcccaagatc tcccaggatc attttgatct ctttcatttt
     1081 cccggcattt ccggttgcaa aaacaattct tttcttcatg attttctccc ttctgctttt
     1141 gttcatccta tattcgttat catagcacat cagcgattat ctgtataggc tttcaggcag
     1201 atatttccgc catactcttc ttcgatccgg ctccacctgt aaccgtccac gctgacgtag
     1261 ccgcacccgt cttccagatt tacatttgca gtctggtcat cggcccgata ttccatcgcc
     1321 actggataat cactgcccgg tgtgtttatt ttgaccataa cagcaaaact ttctccttct
     1381 gctatggaaa tttctttttt cagatcaatc gtataaaacc cttttcgttc cagatgacct
     1441 ttctggatct tctttggcaa aacaagtgac atctcgtttt taaaatctgc cgccacatag
     1501 atttcgtaat ccgactccgg cccggttgca taaaacccga ctgcacgcag cgtctgtgta
     1561 ctatctgccg tataaacgtt tgcaaaccag cagctttctt ttttatagcc catatttccg
     1621 acccagccac acagatcggt ctgataaaga tggtcatagt tatccatccc gtcaatcctt
     1681 gtgtatgcca cacagtttct gccaatctgt gtatcatcat aggcaacata aaatcttccg
     1741 ccgtcgccaa agccttcgcc ccagctgttc tgacacagaa acgcaccgtt tttcgatgga
     1801 gtcttcgtaa aattctccgc cggataatca tcatcccatc caatgatcag aatgtcatgg
     1861 ttcacttcat cctctccatt ataatagtag gaagtatttt ccggatcata gtattcagat
     1921 gtcacttttg tcccgcccat atccatatag atcgagcttt gaacggctcc gtaccggtaa
     1981 atcatttctt tgatcgcatc aaaatcctta tcttctgcga tctgaacttc ctgcacatgt
     2041 tttaccggag aaaggccttc cggtgtaaca ccatctccat aaggatccat ctcttcggtc
     2101 accggtccct gccacgccgt cagataggcg atcgcaatgt tgtaggctcc tccctcttcc
     2161 tgaccgattg catagctgtt ctgtgtggaa atgtgatccg ccgaaaaaac gatatcctcc
     2221 tgcggcagca gccgtgattc cagggcagac gaagtaacaa ctgcccagca agtcccaaac
     2281 gtaccttgat ttttcacttc cggagctttg cccgtcatgc gtccatcata agcggacggc
     2341 agaccttctt cctgctgtcc gtctggcttc tgtgcctgtt ttttcgtttt ctgtttcctg
     2401 tttccggttt ctgtggtttc gttcgtctct gtctnnnnnn nnnngatcca gatgcagcag
     2461 ccggtcgagg ggttcagtgt atacatcata caacaacgac acactcaggc attcgctcac
     2521 gatcaccaga gcatttcgca tacttcttaa aaaccttatt ttcaattttt ctcccgtctt
     2581 ggaggttttg gccccagata ctgatagaaa taggttttga tcataccatt atagatctta
     2641 cggttcttgt ccgctttctt tcctatataa tgctgtgcat cctcgtaact ggtgatcata
     2701 tatttggacc aggtatcaag acggtcaaag ctctctccga tctgccggta aagtgccgga
     2761 agttctgctt tttcttccag acgctctcca tacggcggat tggtgatgat aaaaccatac
     2821 tttttcggat gatgcagttc gctgaccggt ctttgctgga aatggatcag ttcttccact
     2881 ccggctcttc tcgcattttc tcttgcactg tgcaccactg ccgcatcgat atcgtatccc
     2941 tgaatgtctg tctcgatatt cagattgatc tgctcctgtg cttcttccac cgcatcgtac
     3001 cagtttttct tcgggatcag atgcagccag gcttctgata aaaagctgcg gttcatgccc
     3061 ggtgccatgt ttgccgccat catggcggcc tcgatcggga aggtaccgct tccgcagaac
     3121 ggatcaacca gaatacggtc ttttctccat ggtgtcagca tgatcagtgc tgctgcaagc
     3181 gtctccgaga tcggggcttt tgctgtattc nnnnnnnnnn gactgaatat ccgaggtact
     3241 gaacagctta ctctttacgg aagttgcctt tgtaacccag aattttccgt tttccggaat
     3301 atattcttcc cacggaagag cacggacacc ctcaaaaagc tcgtcaaacg tctctgcatg
     3361 caccgttccg accttcagaa ggatcctctc cgccgtccgc agaaaaatat ttgcatagca
     3421 gatggcctcg gcatctccga gaaaagtcac ctttccgtct tccactttgc tgatctcata
     3481 acctaaatct gtaatttccc gtttcaatac tgcctccagc ccgaaatggc atggggcgat
     3541 cagttcataa attttcatat tttctccgta tcttcacatc tgtcccttgt ctacagctgc
     3601 agttctttta taatcgttcc atcaaatcct ttgatcgtaa acagaccatc ttccaggatt
     3661 gcataacttg gcggatttcc ttctttcggg atcgataccg atcccgggtt gagcagtgta
     3721 taatcttctc ttttttctgc ttttaataca tgcgtatgac catggatcag aatatccccg
     3781 tttttcagag gcggcagatt gttttcatta taaatatgac catgtgtggc atatagatta
     3841 cgttctccgt aaagaatcag agcgtaatct gccatcaccg ggaaatccag taccatctgg
     3901 tctacttcag cctcgcagtt tccgcgaacc gcacagatat cattcttcat caggtttaac
     3961 atggcaatca cttcttttgg agcatactct ttcggcagat catttcgcgg tccatgatac
     4021 agaatatccc cgagaagaat caggcggcct gcctcctccg cctgatatgc ctccagcatc
     4081 tttctgcaat aatacgcaga accgtgtata tcagatgcaa acatcaattt catatcgtat
     4141 attctccttt tctttttcac tttgcatttc aaggcaaaag aacagctgtc ttttcagccc
     4201 tttttcactg ttctcttcct gtttattaac tataatcttg ctgtttttcc ctgtcaatct
     4261 tttttgcctc tatccaagcc ttctgtgcca
//
LOCUS       DAWDWH010000184         4280 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_8020_length_4280_cov_12.900118, whole genome shotgun sequence.
ACCESSION   DAWDWH010000184 DAWDWH010000000
VERSION     DAWDWH010000184.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8020_length_4280_cov_12.900118"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3502..3511
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1051 a         1098 c          932 g         1189 t
ORIGIN      
        1 atggcaaaat tcagctctat gccggtaact atgacttctg gtatgaatcc agtcagctga
       61 tgatccgtca gatgaaagaa gcaaacaaaa agaaggaaga aaaaatcaag gaattacagg
      121 actttatctc cagattcagt gccaacgctt ccaaatccaa acaggctact tccagaaaac
      181 gtgctctgga aaaaatccag ctggatgaga tccgtccttc cagccgtaaa tatccgtaca
      241 ttgatttccg tccggaacgt gagatcggca atgaagtact gatggtagag aatctttcca
      301 agaccatcga cggcgtaaaa gtactcgatg atatttcttt cacacttgga agaaatgaca
      361 aagtcgcttt tgtcggcggc aacgaacttg caaaaacagt attcttcaag atcctgatgg
      421 gcgaaatgga accggacgaa ggttctttca aatggggtgt caccacatct caggcttatt
      481 ttccaaagga cagcaccaaa gagttcgaca atgatctgac gatcgtagac tggctgacac
      541 agttctccga gatcaaagat gcaacttatg tacgtggctt cctcggacgt atgctgtttg
      601 ccggtgatga cggtgtgaaa caggtccgtg tactctccgg tggtgagaaa gtccgctgcc
      661 tgctctccaa gatgatgatc tccggtgcaa acatcctgat ctttgacgaa cctaccaacc
      721 acctggatat ggaatccatc actgccctga acaacggcat gatcaaattc ccaggtgtga
      781 tcctgtttgc ttcccacgac catcagattg tgcagacaac ggcaaaccgt atcatggaga
      841 ttctgccaaa cggaaaactg atcgacaaga tcacttccta cgacgaatac ctggcaagcg
      901 atgaaatggc aaagaaacgt caggtttaca gcacagaggg aactctggaa gacaactaaa
      961 tcatcaagat cagatctttc tgaatatact ttatgatgcc agggtcaaaa ggtctaaatc
     1021 cacatgagct tgctcatagg tggatgaaag accttgggac ccgcccagat atggagcaga
     1081 acatccagtg gatgttcgtt ttgctccgac cgaagcggag cgtagacata aaagtgggat
     1141 gataatccta cgatatgcga atactggaga ggattgtggg agtgcaacgc acggaacaat
     1201 ccgcaggcat catagttggg gacttttgac acccacatca ctttatcaaa agcaaaaaaa
     1261 ggatatggaa tccattttgg aaaccatatc cttcttttta tgtcaagcgg atattcgcaa
     1321 tccgctttgc tatttggtta aatcaattaa accagaatct ctgcttttac gcccagcagt
     1381 tctttgttct cttccagaat cggattcacc acgtttttca ggaagttgtc cacctgtctt
     1441 ggtgcacggc ctacatatct ggacggctcc atgctcttct gcagctcttc cagtgtcatg
     1501 ttgaatgccg gatctgctgc aatcagttcc agaaggttgt tttctttccc ttcttctttg
     1561 acatgtttgc ctgcttccat ggaaagtaca cggatacgtt catgcagttc ctgacggtca
     1621 ccgccagcct ttacagcatc catcataatg ttctcagttg ccatgaacgg cagttctgcc
     1681 atcatgtgtt tttcgatgac tttcggatat acaaccagac cgtctaccac gttcaggcac
     1741 agatccagga taccgtcgat cgccagaaat ccttccggaa cggaaagtct cttgtttgca
     1801 gaatcatcca gggttctctc aaaccactga gtcgcagatg taattgccgg attcagtgca
     1861 tctaccatca cataacgaga aagagatgcg atacgctcac ttctcatcgg gttacgtttg
     1921 taagccatgg cggaagatcc aatctgggtc ttttcaaacg gttcttctac ttcttttaaa
     1981 tgctgaagca gccggatatc gttggagaat ttgtgtgcac ttgcagcgat gcctgccagt
     2041 acattgatca ctctggtatc gacttttctg gagtaggtct ggccggatac cgggtagcac
     2101 tctttgaatc ccatcttctt tgcaatcatc ggatcgatct tgtcgatggt ctcctgatct
     2161 ccgtcaaaca gttccaaaaa gcttgcctgt gtcccggttg ttcccttgga accgagcagt
     2221 ttcatgcttc caagtacata gtccagatct tcgagatcca gacagaactc ctgcatccaa
     2281 agcgttgcac gttttccgac ggttgtcggc tgtgccggct gaaaatgggt gaatgccagg
     2341 gtcggctgat ttttgtattc atctgcaaat tttgccagct ctgccaatac attgaccagt
     2401 ttctttttca ccagcttcag tgcttctgtc atcacgataa tatcggtgtt gtcaccaaca
     2461 tagcaggagg ttgctcccag atggatgatg ccttttgctt tcgggcactg tacgccatat
     2521 gcatacacat gggacataac gtcgtggcgg acaagacgtt ctctttcttt tgccacatca
     2581 taattgatgt cctctgcatg tgctttcagt tcctcgatct gctcgtctgt aatgttcagt
     2641 ccaagctctt tttctgtctc agcaagtgcg atccagagtt ttctccaggt acggaatttc
     2701 atatccggtg aaaaaatgta ctgcatttcc ttgcttgcat aacgctctga taatgggctt
     2761 acgtatctgt ctgtactcat attttcctcc tgtatctact gttcgaattc tcctctgata
     2821 tcctccatcg gcggatccag cggatagttt ccggtaaaac atccggtgca gatcggcagg
     2881 tctcctgcca tttgacgcag gcgactgcag tccagatatc caagactgtc tgctcctatc
     2941 acctgtctga tctcctccac ggtacggttg tatgcaatca gctgctctct tgcaggcaca
     3001 tcggtaccga agtagcacgg ccataaaaat ggcggtgaac tgacacgcat atgcacttct
     3061 ttggctcctg cctcacgcag catatgcaca atgcggtcac tggttgttcc acggacgatc
     3121 gaatcatcga tcatgatcac tcgtttgcct tctactgctt cacgcagtac attcagtttc
     3181 acacggacgc tggactcacg gctgctctgt ttcggtttga taaaggtacg gccgacatag
     3241 ccgtttttca caaaagcagt accgtatgga atgccggact gaagggaata tccaagtgct
     3301 gctgcatttc cggattccgg gacaccgact acgatatctg catccaccgg tgaatccatc
     3361 gccagatatc ttcctgccat cagtctggaa tgatagacac tcacaccgtc gatcacgctg
     3421 tccggtcttg caaagtaaat gtactcaaaa acgcagtgtg ccgtctgctg cttttggcac
     3481 agacgtgtgt cagatttgat gnnnnnnnnn ntttgatgcc ttcctgtgtg atcgtaacga
     3541 tctcacccgg cagtacatcc cttacaaact cggcaccgat cgtatccagt gcacatgttt
     3601 cactcgccag tacataggca tttccccttt tgccgatgca cagtgggcgg aacccctgcg
     3661 gatctcttgc tcccatcagt tttctcggag acatgatcac aagagaatag gctcctttga
     3721 tcttctgcat ggcacgggca actgcctctt ctgccgtctt gcttctgaca cgttctctcg
     3781 caatatgata tgcgatcacc tcggagtcaa tggtcgtctg aaaaatcgct ccgccatatg
     3841 ccagctcttc ccgcagctcc ggggcattga tcaggtttcc attgtgtgca agtgcaagcg
     3901 tgcctttgat ataattcagt accagaggct gtgcattctc tcttgtgctg gctcctgccg
     3961 tagaataacg cacatgaccg acaccgatgt ttccatgaag attctccagt ttttctgcat
     4021 caaacacttc gtttacaagt cccattccct tgcaggaatc cacttttcct tttggtcctt
     4081 ccgtatcact gacggcgata ccacagctct cctgtcctcg gtgctgtaaa gcaaacagac
     4141 cataatatat ggtctgtgca atatcgttcc cgtcaaaatc atagatgccg aatacaccac
     4201 attcctcgcc tggtttgtca tccatttctc tctgaatatg caaattctcg ttcatctgat
     4261 ttcctttccg tggtttccat
//
LOCUS       DAWDWH010000185         4277 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_8025_length_4277_cov_20.091426, whole genome shotgun sequence.
ACCESSION   DAWDWH010000185 DAWDWH010000000
VERSION     DAWDWH010000185.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8025_length_4277_cov_20.091426"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          999 a          935 c          953 g         1390 t
ORIGIN      
        1 gtcatattaa ccttgatgct cggagtcaga gctgttccga cagtacgggt ctctttttca
       61 ccacatgcac atactctttc ctgtttttct gctgaaaata cagtagcagc cgatacggtt
      121 ttccatgtgc tgaagctgtg tgcgtgtttt acagattctg tttcagtttc actttcggtt
      181 tcttttccga ttgccattac gatatctttt ggcaggatgt aagcagattc agtgctgtcc
      241 tttggtgcct ttacaacggt gatctggtag gtgccgccgg aaggaatagt aagtgatact
      301 tttccgtttt cgtcggttat ggcacttaca actgcattgc cgtttgcatc aacagcggat
      361 acggtataac ctttctgtac agatgccggt ttgagattgc ccttttcatc gtaatccatc
      421 gggtgcatac cccataaggt cagatccagc ttttcaccgg cctggatggt ttccggtaca
      481 ttttcaaaat acagataaat atctttgtac tgcttcaggt caccatacat aaatacagat
      541 acggaatccc cgctggatac aactgcttct gctacactgg cagatcctgg aatctgatcg
      601 ttgacgcaga taccaatgct gtaggtttca atgccataga tcttgttgat aaatccattg
      661 tttacagcca gatagtcggt agggtttgct ttaaaggcat ctttgtactg tgcggcatgc
      721 catgcgacca gcacatcaag tgcagttact ttgtttttat aagcagccgg tttggaataa
      781 ccatagtttg cagcagtatg agccttaacg gtcattttgc tgacatccat acctttgccg
      841 gcttcctgat tgatctttac ggtgacaaga gcttctgttt cgttctgaga atcctgtatg
      901 gtgttgataa gagcagtgat cttagcaact ttctgctcga tttctgtttc ggctgcatct
      961 tctctttcaa ggagagcttt tgcttctgtg atcgccgtct gcagtttgtc tctggttcct
     1021 tccggatact gtccggctcc ggttccttcg gttacagaat ccagaagcgt ctgtgcatga
     1081 tcaatggcag tctgaagctg ttcagcaact ttgctgtccg gctgtacagt aacagtaacg
     1141 gatactttct gtttgtagag tttctgtaaa gcttcgttgc ccggatggct ggatgcgtat
     1201 tgtcagtaac gttcgctgct gagccaggaa gtgatcgtga tctcagttgg agtttccgga
     1261 cggatgacaa ccagattttc atgctggatc acggcattat tggaagaacg gaatttgttg
     1321 taaccggcac cctccatttc ccatggatca tcgaaatatc cgtctgcaca gataccggca
     1381 tcggtaacat cactgatgtt gtatacccag acagcatttc catcggcatc aaaatacatt
     1441 tcctggaacg gatgcaggtt ttcagtgatt ttatctgcag atacattgtt tcctttgata
     1501 ccgtcaaaat aatgagcttt cgcataatcc atcatttcaa cttctttatc caggtcttcc
     1561 tgtgtcagct ttttgatggt gatcgggaat accctggaaa cagtcacgcc ctcacgcgta
     1621 aaggaaacgg tcaggttgac ggtggtgtcc tgaggctgga atacatctac gtttgctttg
     1681 taaccgttga ttttgacagc atcgttgtca gaagttacgg tgatttcttt attattaaag
     1741 acattctcgc cgttctcatc ttcgatatga gtatatcttg gcagctgaat gtcaccggtg
     1801 caggcttccg gatcaattac cgtcgttgtt ccgtaataaa caagatcggt gatcttataa
     1861 tactggttca aaattgcttt gagttcagct tcagtcggag ccggttttcc ggtacctttt
     1921 acagttacgg taaatggcac gctgatcgta ttaatatcag atattttttc aacctgttcg
     1981 ttcatgacag attcatttgc cgtgaatgta gcggtcagtg taacttccgt atcttcaacc
     2041 ggctgattga tcacaccggt tgcagcgtgg agcgaagaat cggtcgaagg cttctggatg
     2101 ctgatcacat ttgtattgga agaagtccag ctgatggttg accatacggt acgcaggctg
     2161 gttcccatac actgcggcag tgtcagattg gaagttacct ctgtctgact ggtattttca
     2221 cctttgattc tatcccaggt cagctgatcg gcttccgatt tcattttgga agcaaaatgt
     2281 ttctgatccc atccaatgac tactctgcgt tcggctgttt ctgctgtcgc accgtttaag
     2341 ctgaatttca gcacaacgga aatggttttt aaattcagac cataagcgtt cagcgtatcg
     2401 gcagtagagt agtggatgat gccgtcagtg ctgatccagt cagtatcgtc agaagaaaca
     2461 acggataccg ttacctgatc tgctttatcg acagctgcat aatcgccggt tttcagtttt
     2521 gcttcgatca gtgcattaat attagaagcg tttttgtagg atgtgcggat agcaccaggt
     2581 tccttgtcaa agatttcttt taccttatca acggcagcct gatcggcaga atcaaccgtt
     2641 acatctttgt ccatgtttac ggcagccgta atcttttctt ctgatccggt tggtgcataa
     2701 gaaacatttt ttacgtcctt atatccctcg gcagttacag aataacgata agtgccgtat
     2761 ttgctcagcg tgtaggaacc gtcaccattt gctgtcatgg tttcccagtc ggtatctccc
     2821 cagtcatctt caacctcttc ttccactttg aggttaaagt ttgtgatggc tgtatcgccg
     2881 tccattggct ggaaggttac agcgtagctg gtaatatttt tttccagttc tacatcaatg
     2941 gtggtatctt cgtctggtgt gatcgtgccg ttggcagttt tgtagtttgg tgcttcaacc
     3001 gtccagttgt agcttacacc tttctgcagt ttatagcttc cgtcggattc cggactgatc
     3061 gtagtatagt agcccttttt aaccttgatg gaagcaccgg aaatttcagc accgcttgct
     3121 ttgtccttga cggaaaatct gatattctgc atcgtgatcg gacttaaagt tacggttttt
     3181 aagaaaacag gttcttcgga attgcctgta taagtaaatg tttcgctata atcggtatag
     3241 ccctcttttt taacggtcag cttgtaagaa gcaccgtttg ccatcttgta gctgccatcg
     3301 cttgcaggtg taaccttgtc ccatccgtct ttttctacgg taatagaggc atccgtaatt
     3361 ggatttttgt ccttatcctg aacagcaacc tgcatcttgt attctacttc agcggcttcc
     3421 agaataaagg tgaagtcctg tgtggcaagt gcagtggctg ttccgtcgtc gatggcagaa
     3481 ttttcggtat cacttgcgaa cagtttcagt gttgcagtaa aagtatacgg tgatgtcggt
     3541 ctgcttttaa tggaacggct tccgtttttt gtcggtacca aataggagat atatggattg
     3601 tttttatcaa tataccatcc tgttttataa acggtattaa gatatttcag acgtaaggaa
     3661 ttgatggatc cgccgtttgg gattttcagg ccaatattgt aggtaagacc atcctcactt
     3721 ttaacaacac cattaccacc tgtggtgata accttgtctg tagcatcaat aaaattttct
     3781 ttcagatacg cttgtgcggc ttcttctgca ctctgggttt cgtctgcctc ttcagcctgg
     3841 gaaaccaccc cggtatctgt cggcgaaaga tcgctggcat acgtcactga cggcagattg
     3901 ccaactgtca tcatggcaca taaagccagg gaaagtgctc gcttcattag ctcttttctt
     3961 ttcacttttt ttttctcctg agtttgaaat agaatgaaaa tagttatagg gtatacagga
     4021 tatcctagcc ttttccaatt gctgtgtcaa gaatttaacg agagaaaaac ttacattctg
     4081 ctatccaaaa tgtccaaagt gacaaaagaa ggattacaaa aaaagtttaa aaaaacaaaa
     4141 aatttctctt gcatttctgt gaaagctatg ctattatagg cagtgcattg agagatgcgg
     4201 gtgtagttca atggtagaac accagccttc caagctggat acgtgggttc gattcccatc
     4261 acccgctttt ttgttgc
//
LOCUS       DAWDWH010000186         4277 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_8026_length_4277_cov_10.639507, whole genome shotgun sequence.
ACCESSION   DAWDWH010000186 DAWDWH010000000
VERSION     DAWDWH010000186.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8026_length_4277_cov_10.639507"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1234 a          832 c         1105 g         1106 t
ORIGIN      
        1 atgccgcagt gggagaaata tacgaagtgg ctccagggaa ccgtaaataa aaaggtatgt
       61 gttttggagc ttggtgttgg ttttgaatat ccgacggtga tccgttttgc ttttgagaag
      121 attgtatatt ttaatcagaa atgtcatatg taccgtattc atgagaaatt tgcacaactg
      181 actccggaaa tcaaggatcg aacaacggca gtaaaagaaa atgcagtaaa gcttttgctt
      241 gaggatggag cagatgtcag ataatcatcg acaggatttt gcaaatatga tatgatgatg
      301 tgggagaaaa ttgcgtagca atgagtgaca gaatttactt gtaacactta tatcatatgt
      361 taggtataaa tatatatcct ctggcagatt gcagaggtat tcttgaattt gaaaggtgat
      421 aagaagcgat gatagcgatt attgattacg atgccggtaa cctgaaaagt gtggaaaaag
      481 ctttgcagag tattggtgaa gatacgatcg tttccagaga cagggaagag atactgagag
      541 cagataaagt gatccttccg ggcgtaggat catttggtga tgccatgcag aagctcagag
      601 actataaact ggacgaagtg ataaaagaag taaccgaaag cggaaagcca tttcttggta
      661 tctgtcttgg attgcagctt ctgtttgaga gaagtgaaga aacacctggc gtagagggac
      721 ttggtatttt aaaaggcgaa attttgagaa tcccagatca tgaagggctg aagattccgc
      781 atatgggatg gaattctctg gagcttatca atcagggcag actgtttcag aatcttccgg
      841 cagatccgta tgtctatttc gtgcactctt attatctgaa agcagaggag ccggagatcg
      901 taaaggcgac tacctggtac agcaccacga tccatgcatc ggtagaaaag ggaaatattt
      961 ttgcatgtca gttccatccg gagaagagca gtgatgtcgg actgcagatc ctgcggaatt
     1021 tcgcagcgat cggaaaggag gaaaacggat atgtttacga aaagaatcat tccatgtctg
     1081 gatgttcata acggccgcgt ggtaaaaggt gtgaattttg tgaatctgcg ggatgccggg
     1141 gatccggtgg agattgcggc agcttatgat aaggccggag ccgatgaact ggtatttctg
     1201 gatatcacag cctcttcgga tgccagaaat acggttgttg atatggtacg aaaagtagct
     1261 gagaaagtat ttattccatt taccgtcggc ggaggcatcc gaaccgtaga agattttaag
     1321 gtgcttctgc gtgaaggggc agacaagatt tccatcaatt cttcggcgat caatacaccg
     1381 gagctgatca gtcaggcggc agataaattt ggcagccagt gtgtggttgt agcgattgat
     1441 gcaagaagaa gagaagacgg aagtggttgg aatatctaca aaaacggcgg caggatcgat
     1501 gtcggtatcg atgcggtaga atgggcaatg aaagtggata aattgggtgc aggagaaatt
     1561 cttctgacca gtatggactg cgatggaaca aaagcaggtt acgatctgga actgaccaga
     1621 acaattgcag aaaatgtttc cataccggtg attgcgtccg gaggagccgg cacgatggag
     1681 catttttatg atgcactgac ggaaggaaaa gcagatgcag ctcttgcagc gtctctcttc
     1741 cattataagg agctggaaat ccgtcaggta aaagaatatc tgcgagatcg gaaggtaccg
     1801 gtaagactgt aagagaagta tcatcagaaa aatatagcag taaaatgcca ggataaggag
     1861 gcaaaattgt ggcaccgatc agtaatgact ggctggaacc gctgaaaccg gagttccgta
     1921 agccatatta tgcaaaatta tataaaacag tgatggatga atatcatacg agaagaattt
     1981 atcctccggc agatgatatt tttaatgcat ttgccttaac accacttaag gatgtcaaag
     2041 ttgtaatttt agggcaggat ccttaccatg gggatggaca ggcacatgga ctttgttttt
     2101 cggtgaaacc agatgtagag ataccgccgt ctcttgtaaa tatttataaa gaactgcacg
     2161 atgactgtgg ctgttatatc ccggataatg ggtatctgac gaaatgggcg aaacagggcg
     2221 tattgctgct gaacacggtt ctgaccgtgc gtgcacatca ggcaaattcc cacaggggga
     2281 ttggatggga agaatttacc gatgcagcaa tccgcatcct ggatcagcag gatcgtccaa
     2341 tggtatttct gctgtggggc agaccggctc agatgaaaaa atccatgctg cacagaaatc
     2401 cgaagcatct gatcctggaa gcaccgcatc caagtccgct ttcagcatat cgcggatttt
     2461 ttggctgcaa acatttcagc cagacaaatg aatttttaaa agcaaacggt ctggagccaa
     2521 tcgactggca aatcgagaac cgtgcggagc aaaagacaga agaataagca gaatcgtttg
     2581 aaatgaccag acttcataga gcgtataaag agtatcagaa tacaaaaaag cagagtacat
     2641 gattttgtta tcatagtggc tctgcttttt tatactctgg cattttgtta ttttttcagg
     2701 tttttatttg ctgtggagag catcagtttg attcggttca gctgatttac ttcactggca
     2761 cctggatcgt agtcgatggc aacgatattg gacatcggat actgatgacg cagttctttg
     2821 atgacacctt tgccgactac gtggttcggc aggcatccaa atggctgtgt gcagacgatg
     2881 ttgttgacac cgctgtggat cagctctaac atctctccgg tcaggaacca gccttcaccg
     2941 gtctgatttc cgttggaaac gatcggtttt gcataaccag ccagatcctg gatatgtgca
     3001 ggcggatcaa aatgtttgct tgctgcaaaa gctctggcag ccggtttgcg gaaatattct
     3061 aacagagcga taccggcatt tccaatggtt gcggaagatt tcttcatgcc aagattttca
     3121 gctttaaagt tcgtgttgta gaaacagtag agcaaaaagt cgatcagatc cggcatcacc
     3181 gcttcagccc cctcggattc cagcagatct accagatgat tgttggcagc cggcaggaat
     3241 tttaccagaa tttctcctac aatgccgacg cgaggttttt tctcatctgt aatcggcagg
     3301 ttatcgaagt catggataat atcacggcac agctttttga actgtcccat agtcggatgc
     3361 ttggaagaga caaaatcttt gcagactttt tcccattttt catgcatggc atttgcggaa
     3421 cctttgactt tttcataagg acgcatacgg tagagacaac gcatgaaaat atcgccgaag
     3481 atcgcaccgt acagaccacg catcacaaga ctgtagctga ttttaaatcc cggatttttc
     3541 tcaagaccgc tcaaattgat ggagatgaca gggatctgtt ccatgtctgc ctttttcagt
     3601 gcacggcgga taaagccgat ataattgctg gcacggcagc cgccgcctgt ctgggaaatc
     3661 agaacggcag tcttatttaa gtcatattta ccggacaaca agctgtccat aatctggccg
     3721 acaacgatca gagaaggata gcaagcatcg ttgttgacat atttcagtcc catatccaca
     3781 gcctgacggt catcgttctg catgacaacg aggttgtaac cggctgcacg gaatgcagat
     3841 tccaggatgt taaagtggat cggagacatc tgcgggcaga ggattgtgta gtcttttcgc
     3901 atttcttcgg taaatacaac acggtcgaaa ttggaagcga cgatctcacg tggcagattc
     3961 tgttctctgc ggatacgcag ggcagcaatc aaggaacgta cacggatacg ggcagctcct
     4021 aagttgttta cttcatcaat cttcagacag gtatagatct tgccggagtt tgaaaggata
     4081 tcgttgacac agtctgtagt aacggcatcc agtccgcatc cgaaagagtt cagctggatc
     4141 aggtcaagat catctcttgt cttgacaaag ttggctgcgg catacagacg gctgtggtac
     4201 atccactgat ccataacgat cagagggcgt tccggcagat tgcggttgga tacagaatct
     4261 tcggtcagaa cagccag
//
LOCUS       DAWDWH010000187         4246 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_8084_length_4246_cov_12.757099, whole genome shotgun sequence.
ACCESSION   DAWDWH010000187 DAWDWH010000000
VERSION     DAWDWH010000187.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8084_length_4246_cov_12.757099"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    801..810
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          970 a         1187 c          813 g         1266 t
ORIGIN      
        1 aaacgacttt cttccagacg gttctgcagt gcctttttga cattatacac ctgatccgcc
       61 tcatcttcat ccagacgggc tatagtataa tgtacggtat agtatatttc cagctcctca
      121 taacattcct cacagctatc cacatgttcc agaaagtctt ccagatcttt gtcattgagt
      181 tttctcataa tataagctgt taccaatgac tgggcttttt tacaatccat cgtgatcccc
      241 cgctgtcttt tcctttgttt ttcgttcccc ctcagtatat cctatctccg ttcgggtgta
      301 aagtaaaaga aaacacgcaa aaaacgcttg caataccaga atatttatgg taaactaaat
      361 atcggttttg agattttgcc ggtgtggcgg aattggcaga cgcacttgac tcaaaatcaa
      421 gcggtttaac agccgtgccg gttcgagtcc ggccaccggc atgaaaaaag tcctgaagaa
      481 cattcaggac tttttttaat cgaatctgcc actgatacaa gcctgtagtg caccgcctgc
      541 caagacggga ttcatctcac atttgatata cagtcacctg aaagatccag tagatcagtc
      601 ccagcaccca gctcgagaag atcccataca ggatcacagg tcctgcaatc gtaaagatct
      661 tgcatccgat cccgaaaacc tgcccttctt tctgatattc gatcgcagaa gctgccaccg
      721 aattggcaaa tccagtgatc ggcaccagcg ttcctgctcc gccgaacttt gcaagtttcg
      781 gataaatatt cagaccggtg nnnnnnnnnn ctgacaaaaa tcaggatcag ggaacaccag
      841 ctcgctgcaa tgtctttttc cagtccgagc atatccccat aatttaagat tgcctgtccg
      901 atcacgcaga tcacgccgcc tgtcacaaac gctttgagca tattcaacgg cagactgtgc
      961 accggtgtca cccgtttgac ataatcattg tatttctgat ttctctgttc ttttaacaca
     1021 tctttcatgt tcttaacatt ccttcctgcc atctgctttc tacatgcccc aacgcctgta
     1081 aaaatggtac agactgcccg ccgtttttcc ggctgcgatc gcgatcacaa tcagactgta
     1141 accttttgta atgccgatcc ttctggcaaa gatgggaaaa atattgacaa tttctgccag
     1201 tgccaggatc catccaccaa caaaaatccc aaacagcaca cccatcagca caagaccaaa
     1261 aattcccagt ggaatccgga tctgatgcac cgtcagaaga tttccgaaaa tggctcctgc
     1321 aagtatcgct gtctcatatc ctttcacatg ctttgccgta tgtgtgatcc cgacaaaccg
     1381 cgtgatgatc ccaagtccca ccatcagggc gatcacaccg cctgctatga caaaacctcc
     1441 ggagagtccc acgactgccg gaatgatctg tttttccgcc atatcatcca cccgcctttt
     1501 tctgctgatt tgtgtttttc tgttttttcg ggtcacgggt gcttcgctcg atcagcgtcg
     1561 tgtcgatctc atcttcgtaa gtacgcatct ccacttccag aggtgtcgga tctgccgtga
     1621 tctttctgcc gccgaaatga ttgaaaaaga gcagtatccc aagccccagc ccaatcgagt
     1681 acgagaactc cataaccgta aaaccactgg acggtgtgcc cgtaaactgc tgatagatct
     1741 gagcaaacag ctttggaatg tccacatcgt tgttaaacgt catgatcgta aaagccgccc
     1801 caaaaaacga cagtgccgcg acaaatatgg tttttgcgta actgagcaca cgccccggct
     1861 gtttttccgc ttcatacgtc acaaggaaat catactctcc gatgttgttg atctcaagcc
     1921 cggaaacttc ttcctggatc cgctcaatca gctccatgac cgacatcgta tgccgccccg
     1981 gtttcccgtt tgtcgcctgc ggcactttga tggttttcac aagattttct gtctgtttat
     2041 ctgtacagag aatctgtgcg agatcaccaa tcgttatttt tttgtcatgt acctgcacat
     2101 ttcgatccac tttcagataa agtgtctcac cgcctgcctg cttcatatcg tagcctccag
     2161 ctgtattttt ctttcttctg tagtatctcc tttgttttgt tttttattcc cgcttatcat
     2221 ccctttcttt ttcccagaat ccgcctttac agccgttccc tcagtttccc cagaatcttc
     2281 ttttccatcc tggaaacctg cacctgtgtc atatccagtt gttttgcgac ctgcacctgc
     2341 gtcaggtctt caaaatagcg aagccgcaga agatcacgtt ctttttcttc cagcgtacca
     2401 agaagactgt ttaaaagcat atgatccaga agttcttctg aggcatcttt ttcttctttt
     2461 ataccgtcca tcagagaaat ggcatttccg tccccctggt aaatggtctt gtgcagcgat
     2521 tctacctcgc ttgcagactc catcgccatc acgatctctt ctcttgtcgc cccgatctct
     2581 tctgaaactt cttccagggt tggttccctc ccgtaatcct tttccagctt ttctcttgtc
     2641 atgcacactt ttaccgccat ctcttttaaa gagcggctga ccttgatcat cccgtcgtcc
     2701 cgcagaaacc gtttgatctc tcctgcgatc atcggaactg catacgtcga aaatctgaca
     2761 tcataggaaa ggtcaaattt atcgattgct tttaacagtc cgatgcttcc aatctgtatc
     2821 agatcttcca tctcacatcc tctgccgtta aaacgcctcg cgacggtata gacaagtccc
     2881 atattctgct gtgcgagtat attccttgca tctttgtccc ccttgtgaga acgttcgatc
     2941 aatgcaaggg tttgttccat ccgatctcct ttcgcatgga aaccgtggtt ccatgtccag
     3001 gtgtggattc gacatgcact tcatccataa aagcttccat aaaggcaaat cccataccgg
     3061 aacgttccat ctccggtctg gtcgtaaaca gtggttccat ggctttttca atgtccggga
     3121 tgccgcatcc ggtatctttg accgacaggc agagggcatt tccttcgata tgacaggata
     3181 ccgttatttt ctccaccttt ccgtcatatg catgtacgat acagtttgtg actgcttctg
     3241 aaactgctgt ctttacatcc gccacctctt ccagcgtcgg attgagctgt gtcataaaag
     3301 cggaaactgc cacgcggata aacccttcgt tgcaggagcg actgtcaaac tctatcttca
     3361 tttcgttttc ataccccatg tctgttccct ccatacatcg ccccggttgt ctgtggggta
     3421 tcctcgtaaa tcccgacaat tttatagatt ccggaaagca gcaggatcct gcgtaccctc
     3481 tggtttacat gaattgccgt tacctttcct ccaagcgact gcatcagttt ataacgaccc
     3541 atgatcatgc cgatcccggc actgtccata aacaccgtct ccctaaaatc aaagatcacc
     3601 tgtttgactc tccggcttcc catcagctga tctgcctccc ggcgtatttt ctctgcaccg
     3661 tgatgatcca gctcttccgg aacaaaaatg atcatgtcgc tccctctcat tttcacaaaa
     3721 tcctgcatct ttctgatatc ccccaatctt ttccgtatac tgttgcttca agtcgaacgc
     3781 acgtgagact tggcacgcga ttttggtcag ctttgctgac atttacccaa cagaatcgct
     3841 gtgcattcta cacttcgttc cggtcggcac aaaaccattg tccactggac aatgtgtgcc
     3901 ctcgcggagc gtttatctta gtcggagatt gcacttccac taagacaaat ttgttgctcg
     3961 ccacttatag ggagtcttct ccgactaaga taaaaaaaga agccctcacc gatctttttc
     4021 gatctgtgta agcttctctt ttgtatttca tattttctta ttcgatgcca aacatgttgt
     4081 tgatatccgc agtaccgtta tcgcctgtgg tatcgttgcc ggtcgtatca tttccggtcg
     4141 tatcagccgc cccggtggtg ttatccgttg tcccggctgc atttgtggta tcggttgttc
     4201 cggctgtatt tgtggtgtcc gttcctccgg tcgtcgtacc ctgtgt
//
LOCUS       DAWDWH010000188         4240 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_8096_length_4240_cov_10.902509, whole genome shotgun sequence.
ACCESSION   DAWDWH010000188 DAWDWH010000000
VERSION     DAWDWH010000188.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8096_length_4240_cov_10.902509"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4000..4009
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1299 a          865 c         1061 g         1005 t
ORIGIN      
        1 tcggacacgg agacgacctg tttaaactga tcatgccgct tggcatttcc tttttcacat
       61 ttcagattct gtcctatctg atcgatgttt acaaggggaa agtaaaggca cagaagaatt
      121 ttctgaaata tctgctttat atttctttct tcccaagtgt cgttcaggga ccgatcccgc
      181 gttatgcaga tcttggcaca cagctgtacg aagagcatcg ttttgagtat gataacctca
      241 gggacggagc acttctgatc ctctggggat ttgcaaagaa gctgatcctt gcagaacgtc
      301 tcggtacgtt tgtagatcag atctatggaa attatacgca gtacaccggt atgctctttt
      361 tctttgtggc gacggcagct ttttccatcc agatctacgc tgatttctcg ggatgtatgg
      421 atattgcgac cggaacggca agactgtttg gcatccgtct ggcaccgaac tttttgcgtc
      481 cgtatttttc aaagacgatg ccggagttct ggagacgctg gcatgtgacg cttggaaact
      541 ggtttaaaga ctatgtattc tatccgatct caatctccaa attttcactg gatttaaata
      601 aaaaggcgag aaaacgtttc ggcaatgaat ttggaagaat cgtctcctcg gcgattccga
      661 tcttggcggt ctggcttctg accggaatct ggcacggacc ggactggaaa tatgtgacct
      721 ggggactgtt tcacggtatc ctgatcatgc tgagcatgat cttcacacca tacaacgaaa
      781 aactggtgca gaaattacat atcaaaacag aatgtttcag tttccgtctt ttccagatgg
      841 gaaggacctt cctgctttgc tgtctcggac gagttttctt ccgggcagat gatttcgcaa
      901 gtgcgatcgg catcttaaag agagcctgca ccggcatcgg atggtaccgc ctggcaaacg
      961 gcagaatcta caattatggc ttaaacaagg cgaatatgac cgttgtgatc gttgcaatgc
     1021 tggtacttct gaccgtcagt atcctgcagg aaaagatgga tgtcctggaa gcactgaaaa
     1081 aacagaatct ggtctttcgc tgggtactga tctatgcact tctgcttgcg gttgttatct
     1141 ttggcatgta cggccctggg tatgatcctt ccgcatttat ctatgagaag ttttaagggg
     1201 aggcgtggca gatgaagata aaaaagcaga tcaaatacat tttcggaacc gctgtatttc
     1261 ttgcaatcgc agctgttctg ctgacgctga ccacctatgt gatgcgtccg acggacaaag
     1321 attttttcag ggcgagagta gctggatttt atgcagaaga cagggacagc ctggatgtgg
     1381 taggctttgg gagcagtgca ctttaccgtt atctgaacag tccgctgctc tggaaacagc
     1441 agggcatcac atcatacggg atcgccacgg cagcgcagcc ggtctatgtg ataccgtcac
     1501 tgatcgatga agtagaaaaa acacagtcac cgcagctgta tattatagaa accagacgtt
     1561 ttacagatac agacaatgaa gtgatcaaag aaaatcgtct tcgccagatc gcagataata
     1621 tgaaagtttc ctggaaccgt tttaagatag tcacagaact gaccgaagga tttgaagatc
     1681 ggctcagcta tggttttgac ctgataaaat accatggaaa ctgggaaaat ctgacagaag
     1741 aatcttggga atatctgttt aatgcaaaag aacatccgct gaaaggatgg tcggtcaaca
     1801 gccgtcataa gatcataagt tatctggatg tgaaagacat taccggagaa ataccgatca
     1861 cagaaaaatc cgaaaaagaa ctgcgggaaa ttatagaaaa atgtaaaaaa gaaaatcttc
     1921 aggtactttt tgtggcaaca ccatggcagg tgacagagga agaacatcag aaagacaact
     1981 acatcaaacg cattgtggaa gaagcaggct tccggttcct ggacggcaat tactatgcag
     2041 aagaaatggg gctggaatat aacgtggatt tttacaatcc aaagcatacc aatgcgatcg
     2101 gtgcagcgaa atttaccaca tacctgggca attatatcag ggaacattat gaccacagca
     2161 aagaggtggc ggcaagctgg gataaggcgg ccgaaaaaga agaaaaagaa tatgagaagg
     2221 cagccgaaaa gatactgaaa aaggcagtaa aagcaatcaa agcagcaaag gctgatcaga
     2281 cagagcagca gtagtacagc cgaagaaaag attgatttat gaagtttaaa gcgaaaacag
     2341 aaagatcaga tcaaaagaaa gaaggggagc atatggtaag atccatcgtg gaagcactgg
     2401 cagaatatgc aaaagtacag ccggacaaac tgtttctggc agatggaaaa gtggaactga
     2461 cttatctgga tgcctggaaa tgtgtgtacg gttatgccgt gcatttaaga gaacttggca
     2521 tccgaagagg agaccatgtc gttgtgcgaa actcacagaa tgcccagctg gtgatcagtc
     2581 agctggcgat ccagcttgtc ggagcagtct ttgtgccgat cgagaagaat gcggcagatg
     2641 accgcatcgc agagatcatc ggggcagtgg aggcaaaatt atatctggca gcgaagcagg
     2701 gtgagttttc ctgcaaatgg gaaacgataa aagatgtatt atcgtataaa atggacgatg
     2761 ccgcagaaat gcgaatatcc gaaagcgatg atgaaaatgg agacagtttt cgctttcctg
     2821 agcctgaaga aaccgcagaa gttctcttta caaccggaac aaccggaaaa tccaagggca
     2881 ttgagcttac ttatgagagt gtgatcgccg tttctgaaaa tgtgattggt tccgtcgaaa
     2941 tgaaaaaaga cagtgtcgaa ctgatcccgg tacccctgag ccattcgcac ggacttcgcc
     3001 gttattattc caatatttta aatggcggca gcgtggtgat catcgacggc gtgatcttta
     3061 caaagaaatt tttcggagcg atcgaaaaat atcatgtgac agcgatagac cttgtaccgg
     3121 cagcactggc ggcactgttc aagttaagcg gagacaaact cggtgaatat gcagaccagc
     3181 tcgattatgt gcagcttggt tccgcaccga tcccgaacca cgacaaggag catctgcgag
     3241 agcttcttcc gaacacacgc ctctataatt tttacggaac aacagagtcc ggatgctcct
     3301 gtatcctgga tttcaatgca atgccggata agaaaaactg catcggaaaa ccgtcctgcc
     3361 atgcagaatt tgtctttttt gatgagcagg gcaatgcaat cgatgcgacc gaagagacgc
     3421 cgggctttct cgcctgccgc ggaaccatga atatgaaggg ttattatcat gaaccgcagc
     3481 tcaatcagga aatcatgcgt gatgactata tctgcaccaa agatcttgct tatgtgggcg
     3541 aggacggact gatctatatg ctgggcagaa aagatgatgt catcataacc ggcggcaaca
     3601 aagtcgctcc ggatgagatt gaagagatcg ccgggggatt tgaaggaata gccgactgtg
     3661 cctgcattcc ggtgccacat ccgctccttg gggcagagcc aaagctcttt gtggagatgg
     3721 aaaacggtgc cgcatttgat gagcagggca tttatgagta tctgcagggc aaactggagg
     3781 catataaagt gccaaaactc atcgagcaga tcgacaagat cccgagaacc tataacggaa
     3841 aattgcagcg taaaaaactg atcgaagccg agaaagaaaa gacagagtaa aaaccggact
     3901 gttatattaa aaaaataata caaaatcaaa ggagacaaaa accatgaacg aattattaga
     3961 catcttaaaa gaagcaatgc cgaaagtaga ctgggaaaan nnnnnnnnna cctcgtagat
     4021 gacggagtga tcgactccat ggacgttgta acgatcatct cagagatcac tgatgcgtat
     4081 gatatcgaga ttccatccga agaaatggaa ccggaaaact tcaattccgc aaaggcaatc
     4141 tatgaaatga tccagagact ggaagaagaa taaaatttat cttagtcgga gaagactccc
     4201 acttctataa gtggcgagca acaaatttgt cttagtggga
//
LOCUS       DAWDWH010000189         4214 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_8149_length_4214_cov_10.466218, whole genome shotgun sequence.
ACCESSION   DAWDWH010000189 DAWDWH010000000
VERSION     DAWDWH010000189.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8149_length_4214_cov_10.466218"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    742..751
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1271 a          832 c         1107 g          994 t
ORIGIN      
        1 ataccctgga tgagctgaaa tactattatc cgcagctgga gctggaactg aaccgtgatg
       61 ttgcatttgt cacaacacag gagctggagg accgttatcc ggatctgaca ccgaagcagc
      121 gtgaaaatgt gtttgtaaaa gaacacggaa ctgcttttat catgaagatc ggtgacaaac
      181 tgaagtccgg tgagccacat gacggcagag caccggacta cgatgactgg gcactgaacg
      241 gagatattct gttctggcat gagacactgg gctgtgccct ggaggtatct tccatgggaa
      301 tccgtgtcga tgcaaaatct ctggcagagc agctgacaaa agcaggatgc gaagatcgaa
      361 gagaactttt gttccacaaa atgcttctgg aagagaaact gcctctgacg atcggtggtg
      421 gtatcggaca gtccagagtc tgcatgcttc tgctcggtaa ggcacatgtc ggcgaagtac
      481 aggcatcgat ctgggatgaa gagacactga acgcatgtga aaaggcagga atctgcttgt
      541 tatgatcgat cagaaatgcc gtctctgtcc gcgggaatgt aaggcagaga gggaaaatgg
      601 acagatgggt ttctgcggta tgacaggaaa aggcatcagg gcggcgagag ctgcccttca
      661 tttttgggaa gaaccatgta tttccgggaa gggcggttcc ggtgccgtct tttttgcagg
      721 ctgtaccctg cgttgtgttt annnnnnnnn ngtgtttact gtcagaaccg cgagatcgca
      781 gccgggagga tgggagagga gatcagcctt tcacgcctgg tggacatctt ttttgaactc
      841 aaagaacagg gagcggaaaa tatcaatctt gtcacaccga gccattatgc ggacaagatc
      901 atgtccgcaa tccgtatggc aaaagagaga ggatttgacc ttccgtttgt ttataactgc
      961 agcggatatg agaaagttga agtcttaaaa cagttgactg gcctgattga tatttatctt
     1021 acggatttta agtatatgtc gtcggaaagt gccctgcgat attcgcatgc caaagattat
     1081 ccaaaggctg caaaggcggc acttgccgag atgatgcgtc agcagccgca gacgatatac
     1141 gatgaacatg gcatgatgaa gcgtggtgtg ctggtccgcc atctgctttt gccgtcgcac
     1201 cgtaaggaag cagaacaggt ggtaaaatat gtatacgata cttataaaga tcaggcggcg
     1261 atcagcctga tgtgtcagta tacaccgctt ggcggacttg ataactatcc ggaactgaat
     1321 cggagagtga caaaaagaga gtacgaaaag ctgctggatt ttgctgtcga acttggcgtg
     1381 gagaacggct acatgcagga aatggaaagt gcgaaagtct gctatattcc accgtttgac
     1441 ggagagggga taaaagcata aaatatgcag taatttgact ttctatgcac caattccgtt
     1501 gacattactt tgaaacagtg ttaaactcag acgcagagca aggaagcagt ccggatgatg
     1561 gaaaagacag aaatttcgga caagcttatt aaaattcagg aggaatagaa attatgaaaa
     1621 aactgattgc agcagcactg gttggaacaa tggtacttac tatggcagga tgcggaagca
     1681 agactgcaga aacaacagga agcgaagctt cttctgatgc agcaggcggc acactggtta
     1741 tggctaccaa tgcagagttc ccgccatatg aatattatga aggcgaaaat gtcgtaggta
     1801 tcgatgcaga tatcgcagca gccgtagcag acaaactggg tatggagctg aagatcgaag
     1861 atatggcatt tgattctatc atcccggctg taacatccgg taaggcagat attggactgg
     1921 ccggtatgac cgttaccgaa gaccgcctgg aaaatgtgga tttctctgat acttacgcac
     1981 atgcaacaca ggttgttatc gtaaaagaag acagcgatat tgcaagtccg gatgacctgg
     2041 aaggcaaaaa gatcggtgta cagcttggta ccaccggtga tatttatgca ggtgatatca
     2101 aagatgcaac cgtagagcgt tacaacaaag gtttcgaggc cgtacaggca cttactcagg
     2161 acaaaatcga tgccgttgtc atcgacggag aaccggcaaa agtattcgtg aaagaaaacg
     2221 aaggcctgaa gatgcttgat gaagcattta ccgaagaaga ttatgcgatc gcagtagcaa
     2281 aaggaaatga tgaactgctg gataagatca acactgcact cggcgaactg aaagattccg
     2341 gtgagctgga taagatcgtt gacaagtata tcacagcgga ataaaagaaa gtgacagagg
     2401 aatgccatgt caagacaatt atacttgaat tttattaaag ataacagatg gaaatacatt
     2461 gtgaacggtc tgggaacgac actgcagatt accttttttg cagtcattct cggtattgct
     2521 ctcggattcc tgatcgcagt catccgttca acctatgaca agacaggaaa agggaaaata
     2581 ttgaattttc tggcaaaagt atatcttacc gtaatacgag gaacgccggt actggtacag
     2641 ctccttatca tttactttgt cgtatttgca agcgtggata tcaataaaat catcgttgca
     2701 gtcatggcat tcggtatcaa ctccggtgcg tacgtggcag agatcataag aagcggtatc
     2761 atgtcgattg atgacggaca gtttgaggca ggccgaagcc tgggctttaa ctatacacag
     2821 accatgatct acattatcct gccgcaggca ctgaaaaacg tgcttccggc actggcaaac
     2881 gagtttatcg tacttttaaa agaaacttcc gtatccggat acatagccat tcaggatctg
     2941 acgaaaggcg gagatatcat tcgaagccag acatacaatg ctctgttccc gcttcttatg
     3001 gtggcagcga tctatctgat catggtgctt ttctttgaaa agctggtatc cattttagag
     3061 aggaggctgc gtaacagtga gcgttgagaa aaaagagacc ctgatccagg ttcagaacct
     3121 gaccaagact ttcggcggca gcgtacatgc actgaacggt gtcagcaccg acatcaaaaa
     3181 aggcgaggtt gtctgtgtga tcggaccttc cggttccggt aaaagtacat ttcttcgctg
     3241 tctgaatcgt ctggaagatc cgacggacgg acgtatcatt tttgcggggg aagatctgat
     3301 ggatccgaag acggacattg atatgcaccg tcagaaaatg ggaatggtat ttcagcattt
     3361 caacctgttt ccgcatatgt cgatcctgaa aaatatgacg atcgccccga tgaaactgca
     3421 gaaacgcggc aaagacgaag cagaaggtca ggctatgaaa cttctggaga gagtaggact
     3481 tgctgaccgt gcaaatgctt atccaagcca gctctccggc ggacagaaac agcgaatcgc
     3541 catcgtgcgt gccctgtgta tgaatccgga agtgatgctt tttgacgagc cgacttccgc
     3601 acttgacccg gagatggtcg gagaagtgtt aaatgttatg cgagatcttg ccaaagagca
     3661 catgaccatg gtcgtggtaa cccatgaaat gggatttgca agagaagttt ccgaccgtgt
     3721 gatctttatg gcggaaggaa agatcgtaga agaaggaacg ccggaagaga tctttacggc
     3781 accaaagaca gagagacttc agagtttcct gagtaaagtt ctttaaagag aatttatcga
     3841 aatacagaaa acggcatggg tgtcagaaaa gctccggtgc cgttttttta tgctgacaga
     3901 tgaaaaatta tgcgaaaaga aatgcttgca tattaaacag gcatcgctat aataatagaa
     3961 gaaacgtaat gatacagtgt agcaggagca tttgctgtcc ggactgtaaa aatctaaaag
     4021 caaagtggtg gataaaattg aactatacag aagcagtaga atatatactt aatgtaccaa
     4081 aatttacgac aaaaaacaaa ccggaacata ccgtagagct gttaaaaaga ctgggacatc
     4141 cagagtgcgg catgaaggtg atccatgttg caggaaccaa tggaaaaggt tctgtctgtg
     4201 cctttctttc agag
//
LOCUS       DAWDWH010000190         4180 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_8216_length_4180_cov_11.513939, whole genome shotgun sequence.
ACCESSION   DAWDWH010000190 DAWDWH010000000
VERSION     DAWDWH010000190.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8216_length_4180_cov_11.513939"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1770..1779
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          856 a         1053 c          831 g         1430 t
ORIGIN      
        1 cagttttctg ccagaaaatc ttccggtttg tccagaaact ctttataatc tgcttcgatc
       61 ttgtccattg tcttttcgaa ctcttcatca aacgaaatac catactcact ggaaagttca
      121 cctaaataaa cactgtttcc ggcaatgata ttggaagcat aatcggctgc cgcatcagaa
      181 tgatccacct gttcgctctc atcatccggt ttctgaatgt cacctttctg actgtccttg
      241 tcattcgcat cgtctttttt atttttatcg tccttactgt caccgtctga cttcttgtcg
      301 ctgtcttttt tatccgaacc atcgtctttt ttatcgctgt ccgatgtacc atcctctttg
      361 gatgcatccg tatcccctgt ctgagatgca tttgtttctg tttcggctgt tgccgtctca
      421 gtctgccgcg tttccagttc cggcttcgtt tccggctgac tggtctcagt ctccgttgcc
      481 ggtgtctcgc tctggctcgt ctctggctga ctggtttctg tctgagagcc ttccttgtta
      541 ctctccgcct gcaaactcga tgcataagcc accggtgctg tactcatcag catactcccc
      601 gccagcatca tgcagcctaa tttatataaa tatctgtttt tcattcatct tgcctttctt
      661 ctataacctg aacacctttt acccctgaca ttataatttt taattataga agtttttgga
      721 tgaattttca agtagtgaca cagaattttc acagatgacc tttcattgct ctcctatttt
      781 tctattacac attgatattt tttactgctt ttatcataat taatgccttc ttctatgtga
      841 acactttccc ttatttttgc tgataccaga cgatcgacct cctgccaaaa aagaccaggc
      901 ttccacctct tggtttctgc ctgatctttc tgatatacta tgtattggat gcgatatgat
      961 ttgtaaagcg gacattcaca atccgcttct ctgcgttcaa tctgtgatta gtcattgtat
     1021 ccgtttggat tctggctctg ccatctccag gaatctgcac acatatcttc gatgccgtat
     1081 tctgcttccc agccaagctc tttctttgct ttttccggtg cacagtagca ttttgcgata
     1141 tcgccaggtc ttctcggttt gatctggtat ttgatcggat gtccgcatgc tttttcgaat
     1201 gcatgtacaa cctgcagaac gctgtagccg tttccagtac ctaagttgta gatgcttacg
     1261 ccttctttgt cagccagttt ctggattgct tttacatggc cgcgtgccag gtctaccacg
     1321 tggatgtagt cacgtacgcc ggttccgtcc ggtgtatcgt agtcatcgcc gaatacaccg
     1381 aggcattcca gtttgccgat cgctacctgt gctacatacg gaagcaggtt gttcgggata
     1441 cctttcggat cttctccgat catgccgctc ttgtgtgcac cgatcgggtt gaagtaacga
     1501 agcaggatca cgttccattc cggatctgct ttctggatat cggtcaggat ctgttccagc
     1561 atccattttg tccagccgta tgggttggtg caggtgccct tcgggcattc ttctgtgatc
     1621 gggatcattg ccggatcacc gtatacggtt gcggatgaac tgaagatgat gttctttacg
     1681 ccatgatttc tcatgacatc gcacagtgtc agtgtacctg cgatgttgtt ctggtaatat
     1741 tccagaggtt ttacggtgga ttctcctacn nnnnnnnnnt ctcctactgc tttcagtcct
     1801 gcgaaatgaa tgactgcctg tacatcttct ttatcaaaga tagcatccat tgcttctttg
     1861 tcgcagatgt ctgcttcgta gaatgttact tttttgcctg tgatctcttc tacacgatct
     1921 actgcttttt tatttgcatt gtacagattg tctacgatga ctacttcata gccttcgttc
     1981 agcagttcca cacaggtgtg gcttccaata tatccagctc cgccggttac caaaattctc
     2041 atagtattta cctcctaatc aatttgataa atagtttctt tatatttata ttttcgtgat
     2101 aacaaggtta aacgatgcca tacgaattgt tccatacttc gtactctcac aattctgtca
     2161 tcaccgtttt acagtgttgc gataaatctc atgaaggctt ctcttccttc cggtgtgcat
     2221 ttgtatacac cggcatcttc cagaacgcct acgaatacat gtccaacttc ctctttgagg
     2281 atatccatca cattatcttt gttcagttcc ggatgtttcg gcaggaagga ttctacccag
     2341 tctgcatgtt tttccagagc ttcgttggaa cggatgtctt tcttctcgat gatataatct
     2401 gccagatcct gaagctctgt tttcagtcgt gccggcagga ttgccagacc cataacttcg
     2461 atcagaccga tgttttcttt tttgatgtta tgtttctctg catgcggatg atatacgcca
     2521 agcggatact catctgtcgt caggttgttt ctcagagcca gatccagttc aaacatatcg
     2581 ccgacttttc ttgcgatcgg tgtgatcgtg ttatgtggct ctccatctgt ctcagcaaag
     2641 atgtttgcat cggcgtcggt atagcctctc catgcaccaa gtacatggtc agccagatcg
     2701 atcagacgtt tctcgtcttt gcagcgaata cgcagtacgg acagcggcca ttttacgatg
     2761 ccggcttcca catcctcgta tcccgggatc tcaacgtgtt tctcgatcgg tgcttttgcc
     2821 attgcaaact ggtaattgcc gccctgatag tggtcatggc ttaagataga gccgcctacg
     2881 atcggcaggt ctgcattgga tccgaggaag tagtgtggga acagtttgat aaagtcaaac
     2941 agtttcacga acgttgcttt ctcgatcttc atcgggatat gctctccgtt gaatacgata
     3001 cagtgctcat tataatatac atacggtgaa tactggaatc cccactgtgt tccgttcact
     3061 gtgatcggga tgatcctgtg gttctgtctt gccggatggt tgacacgtcc tgcataacct
     3121 tcgttctgac ggcagagcag acattttgga taaccgctct gttttgccag ttttgctgct
     3181 gcgatcgctt tcggatcttt ttccggtttg gataagttga tggaaatatc aatgtctccg
     3241 tactgacttg gcactttcca gcgaatatcc tttttgatac gataacggcg gatgtagtta
     3301 gtgtcctggc ttaatttata atagtagtct gttgctttta ccggtgactc tttgtacagc
     3361 tctttgaacg ttccgatcac ctgtgccgga cgcggaacca ggcagttcat gatctttgtg
     3421 tcaaacaggt cacggtaaac aacactgttc tcttttaata cgcctttttc aactgcatag
     3481 tccagcagct cttccagaac ttcttccaga tccacatctt tgaactctgt ctccggctcg
     3541 gtatattctt cctccccaaa cagctccagc agctggttgg tggtataaat acgttcttct
     3601 tccggtacca gtccgctctc tatgccatac tgtaccagac gttttacatt ttcatcaaat
     3661 gtcatgacgt ttcctcctgc ttaaatttct tttgctccgt ctccgatttc cactacatag
     3721 aattcagctt catgtcctgt cttttctttg taagctttgc ccagtttgtc gatgaagtga
     3781 tctactgcat cattctttac gatacttacg gtgcagccgc cgaatccgcc gcctgtgata
     3841 cgggaaccga tcacgccttc ggtctcccat gccagatcta ccagtacgtc gatctcttcg
     3901 caggatactt catagtcatc tctcagagat acatgtgaag cattcatcag tttaccgaat
     3961 ttctctacgt cacctgcatt cagtgctgct acagcctgga tagttctctg gttttcgtat
     4021 acggcatgat gtgctctctt ctggcgaacc ggatctttga tggcatcttt atatttttcg
     4081 aattcttctt cgcttaaatc gccaagtcct ttgatatcta caactgtctg cagctctttt
     4141 aatgctgttt cgcattcgtt tcttctgtca ttgtaagcag
//
LOCUS       DAWDWH010000191         4134 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_8279_length_4134_cov_15.190488, whole genome shotgun sequence.
ACCESSION   DAWDWH010000191 DAWDWH010000000
VERSION     DAWDWH010000191.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8279_length_4134_cov_15.190488"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1338 a          806 c         1042 g          948 t
ORIGIN      
        1 cagaaaaaaa cacataaagg aaatttatgg tataaacagc ttccgtcaca gacagtgcag
       61 gagacctgca agcagctgga taaagcatgg aaatcttttt atgtcctgaa aaagaccgga
      121 gggatcaaag atccaaatcc gccccgtttt aaacaggaca atatcccggt cacatacatg
      181 cagatgggga tccggcatga gaaaggttca gaccagctgc ggctgtctct gccaaaggat
      241 ctgaaaagct acatggaaga aacatacggg atccatgaaa aatttctata tcttgaaaat
      301 aagattttca ggaacatgga tcatataaag cagctgcgga tctatccacc ggaaaacgga
      361 aaatgcgacc ttattgttat ctatgagatg gaagagccgg aacagctttc ccagaatgga
      421 cattatttat ctattgatcc tggacttcat aatctgatga cctgttatga ttccggaaat
      481 ggccgagctt ttattctggg aagaaagtat ctttccctgg aaagatattt tcataaagaa
      541 atcgccagag ttcagtctgt atggtatgca cagcaatcag aaagaggaat aaaatatcca
      601 aaaacatcgg aacatatcca gcggctttac aggaaaaagc agaatacagt gaaggattac
      661 cttcataaag taaccagatg gatcgcagaa tactgcagaa aagaagatat ccgctgtgta
      721 gttgtgggag acattcgaaa tatccgtaaa gaaaaagata tgggacataa gaccaaccag
      781 aagcttcaca gcctgccata taacaggctg tacatcatgc tggaatataa actaaagcgg
      841 tatggaatac aattgataaa gcaggaagaa agctatacca gccagtgcag cccgttatca
      901 ccagaagtat caaaaagata tgcagaagca tcaaaccgaa aagtgcgggg tatgtatata
      961 actgacggag aaagatacaa tgcagatgca gtaggcgcat ttaatatcct gcgaaaatat
     1021 ctttccgtat ccggaaagca taagaaactg tccgtagccg gactaaaaaa tccagaaata
     1081 gtaaaagtag ctgcatagcc gaaaggcaag cagtattggt gtcatggacg caccctgaaa
     1141 aaagacggta tcccgccaga tttcagatgc tctggtaact cagttaccga gtagttcaca
     1201 aaaatacaaa agaaacaaag ggagagggaa atgaagagat tttggaacaa acttacagga
     1261 gaagaacgcc tgtgtcgggc agaacagaag ctgacgatcc tcatcctgga ctgtcaggat
     1321 gaggaaagaa gagtaaaact gatccgcaga aaaaaagaag tacatttaaa gcggctgaag
     1381 atccggagac gaaattatat ttttgcactg attctggcct gtatgatgct catacttgca
     1441 gtgtatgggg gagttcgtgt gatcgggaaa atgatcaaaa acgtaccatc tggtcaaaaa
     1501 cagaccgagc agcctgcaga aagtgaaaca cagaccgaga cggaagatac aacaaggatc
     1561 aatctgacgc ttaactttac cggagactgt attctgggaa cagatgagca gtttgcctgg
     1621 gataccggtt tcaatgctta ctatgaggca aacggtccgg aatatttctt gaagaatgtg
     1681 cgggatattt ttgaaaaaga tgacctgacg atcataaata tggaaggtac gcttacggaa
     1741 gaaaccacac gtgcagacaa acagtttgcc ttcaaaggcg atccggaatt tgtgaagatc
     1801 cttacttcct catcggtgga agcagcaaat atggcaaata accacagcca tgactatgga
     1861 gagcagagct ttcaggatac ggtaaatacc ctggaagaaa atggaatcaa aacctttggc
     1921 tacgatgata cgtcggtgat cgaggtcaaa ggcgtcaaaa tcggattttt cggtatctat
     1981 gagctggatg atcatctgga acgcatacca caggtaaaaa gtgatattgc aaaactcaaa
     2041 gaagacggag cggaaattat tgtggcagtt tttcactgga gcaatgagct tgttactgtc
     2101 ccggatgaaa atcaggtaac gcttgcacat ctggcaatcg atgagggggc tgatgtggtc
     2161 gtcggacatc atccgcatat tatccagggg attggcggtt ataaaggaaa gacgatcgct
     2221 tacagtcttg gtaacttctg ctttggcgga aataccaatc cgacagagat ggatacgatc
     2281 atctttcagc agaaatttac cgtaaacgga aaacatgaga tcgtagatgc gtcttacgaa
     2341 gtcattcctt gcagtgtttc ttcggcagag ggttacaaca attatcagcc gacaccgctt
     2401 accggggagg cagcagatca agttttgcag aaattagaag agcgaagcca gagcatttca
     2461 gaataagcac tttgcgaaaa atagatgtca ggtttcaaga tgtgtcagat caaaggaaaa
     2521 accagaaaac catggtatac tttttcatat aaaagccata ctaaaaccag attccaatca
     2581 aaaacaaagg gcgtgaaata acgtcaaaaa ggcaggaata agattatgaa tagtatgacc
     2641 ggagagcaga gtatctgttt tgagaagccg ccttatcttt ttgtgagtgc ctctgtggca
     2701 ggcaaaaaag aaggagaagg gccgcttgga gaaaaaatcg acttgatctg tgaggatccc
     2761 atgcttgggg aagaaaactg ggaaaaggcg gagagcatgc ttcagataaa agcaatccag
     2821 ttggcactgg aaaaagcagg gaagatgccg caacagattc gctatatttt tgcaggagat
     2881 ttgttagggc agctcattgc cacatctttt ggtgtagaag agctggaaat tccgctgttt
     2941 ggactgtatg gagcctgttc tacctgtgga gagaccctgt cactggcggc aatgacggta
     3001 agtgcagggt atgccgatca ggtacttgcc gtgacttcca gccactttgg aagtgcggaa
     3061 aagcagtttc gatttccgct cggatacggg aatcaaagac ctctttctgc gacctggacc
     3121 gtcaccggaa gcggagcttt tctgctctca gcacagaaaa gccatgcaaa aatcaccgga
     3181 atcacgaccg ggaaagtagt ggactacggt gtgagggatt ctctgaatat gggagcctgc
     3241 atggcaccgg cagcggcaga tacgatctgt cagaatctga tggattttaa ccggcagcca
     3301 caggattatg accgcatcat tacaggagat ctgggctatg tcggccagac gatcctgctg
     3361 gagctgctaa aagagaaagg gtttgatatc aaaaagcagc atatggactg cggaatcgaa
     3421 atttatgacc gtaagttaca ggatacacat tctggcggaa gcggctgtgg atgttccgca
     3481 gtgacgcttt ccgccatgat ccttccgaaa atagaaaaag gagactggaa gcggattctt
     3541 tttgttccga ccggagcact gctctctacg gtcagcttta acgaagggca gaccgtgccc
     3601 gggatcgctc atgcggtcgt gatcgaacat tgttagcgga cagaaaggag cgtatcaaac
     3661 atgagttatg tgacagcatt ctgggtgggc ggactgatct gtgccctgtg tcagatcctt
     3721 cttgaaaaaa caaaactgat gccgggacgt gtgatggtga tcctagtctg cagcggagca
     3781 ctgcttggag cctttaacat ctatcagccg tttgtggaat ttgccggtgc cggtgcttcg
     3841 gtgccgcttc tcggtttcgg aaacgtactc tggaaaggtg ttaaagaagc agtcgatgaa
     3901 aatggatttt tagggatttt tatgggagga tttaaggcaa gtgccgtcgg gatcagtgca
     3961 gctctgatct tttcctatct tgccagcctg atcttccagc caaagatgaa agagtagcag
     4021 aaagcggaca gacgaaagat gaataaaaaa acctacctta ttgaaaaagc agaaaaagga
     4081 ttgacgatac agcaatattt aaaacagatt gccggatttt caaatgcaca gatc
//
LOCUS       DAWDWH010000192         4120 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_8314_length_4120_cov_11.535301, whole genome shotgun sequence.
ACCESSION   DAWDWH010000192 DAWDWH010000000
VERSION     DAWDWH010000192.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8314_length_4120_cov_11.535301"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1137 a          908 c         1001 g         1074 t
ORIGIN      
        1 gtttatttat cttcgggatg ctgatgacga tgttgaagac gagcaaaaga agcagaatta
       61 taatcctctg gcgatttaca gcaaaaaagc ggattttgaa gaatttgcag ggcatttgct
      121 gaccatgatg atcgcagagg cgtccagagc ctttgaacag cttccgattt tgaaaaatgc
      181 agagatactg cgaaatatct tatattccgg tgtctggtgc cgatacgaaa tgatcaaaga
      241 aaaacgcaga cagaaagcag gagaaaaagc agatgaatga tccatatcag attttaggtg
      301 tttcaaggaa tgcaaccgat gacgaaataa agaaagcata tcgcagcctc agtcgaaagt
      361 atcatccgga tgccaatctg gatgatccaa aagaagcgga agaaaagttc aaggaagtac
      421 agcaggctta ccagcagatc atgcaggaga gaagttatgg cggcagtgca gacggaaacg
      481 gtcagggagg ctataacgga gatccgtttg gcggattctg gcagtacggt ggcttcaaca
      541 cgcagcagag gcaggaaacc caggaagata cgcacatgca ggcagcagca aactatatca
      601 ataacgggta ttatcaggag gcactcaatg ttcttagcgg gatctcaagc cgcaatgcca
      661 gatggtatta cttaagtgca cttgcaaatt ccggagtcgg aaataatgta aaagcacagg
      721 aagatatcag tgaagcagta cgcctggaac cgaacaattt ccagtatcag agcttaaaac
      781 agcgtctgga gggcggtacc ggctggtatc agaaacagag taccgtgtat ggaagaccgg
      841 atttctccgg ccaggactgg tgcatgaaga tgtgtctgct caatctgctc tgcaactgct
      901 gctgcaacag aggtatgtgc tgcggtacac cgataggata tcgatgatca ggaaaagagg
      961 gattttaagt gattgatctg acaggatgtg tgtccattcc gtattttaag aaaaccgtgt
     1021 ttacgggaag tgatggaaaa atgagatatc tgctcggaaa agcagaagaa aacgaagaaa
     1081 cggttctgaa agcatggtac tgggaaggac cttatgccag catgtatgtg ccggaggaga
     1141 aaaaaacggc cgcagttttt ccgttttcag aagaaggaat cagccaggcg gttgcctggc
     1201 tgaatgaagc tgcggttaat ttttagcaaa ataccacata tttttaatct gaccggaaat
     1261 ctcctggaaa gtgtaagtct gggaagtgcg tactttgctg gcgggatcat gtaccgtaaa
     1321 agcatcgtcc tgataaccgg tcagcacaat gaaatgtccg gttgtggtaa aaataccagg
     1381 tcccatgctg cagatgatcg gacagccgac cgaaagtaca ttgatcatgg cattttcatc
     1441 cagcccgaga atgcttccgc tgagcccaaa atgctctaca ccattggtca tcaattccca
     1501 tttagtgtcc agattttcat ccagatagcc attggaactt gcataatctg caacgacggc
     1561 aggcgtaatg gaagcatcct gcaaaagacc gctgatcacc atggaaaggg cggtcggacc
     1621 gcatccgctg actgcaatga tcgtttccga accgtaaggg gcatagcccc agcgttcatc
     1681 ccactggatc agggcaggga tctcaccggt tacaaagtca tctccgatgc ttttggcagc
     1741 ggcattttta tgttttttcg gataatccag cacaaaatcc agcatttcgc tgtttttgga
     1801 aagtgctttc agcagcgatt tcggataacg ggaaggatct tttaagatcc tttttaattt
     1861 gggcagatcc gcagactgct gctgcagcag accaagataa tcatccgcag aaacaggggt
     1921 tgtctcagat acatccgttg tctgggactg cttttttttc tttttctgtg ctttttcggt
     1981 tgctgctgcg gtttccgtgt ccagatcgtt tcggctggcc gtttccagag cagcagaaga
     2041 tgctgcctca gagaaacgtt ccgatacgtt accggaagtg gcagtctgtt tctgctgatg
     2101 aacgtaagtc agaaggccaa gcccggtaaa catcaggatt acaccaacgc caagaagaat
     2161 actgaggcgt atgatgtgaa gctgccgttc tctctgctgt tgtcgcagct gacggtattt
     2221 ttctctgtct tgtgtatcca taaattgatc tcctctttgt aaacagtata tcgctttttg
     2281 gacagaaaat cttcaacaca atgtaaacaa tttacgaaaa aagcagaaat atcaggaaat
     2341 cataccttct ttttccagaa tttccagaat ctcccctgca atctctgcgg atgttttctg
     2401 atccgttgcc acacggtaat tagcggctgc ttcataagca ggtctgcgtt tttcaatcag
     2461 ggcttcgatg tctgctgtgt ttttgcggcc ttttaaaagc gggcggtttt catcgtctgc
     2521 cactctttca aggatggttt ccggaagagc tgtgagcagg acgatgatgc ctcatttttt
     2581 catttcacga acattttctt cccgcatggc ggcaccgccg ccgcaggaaa cgagaaaacc
     2641 gccaagagcg gaagtttctt tcagaagatc tgtctcaagc tgacggaaat aagcttctcc
     2701 gtgttcagaa aagatagcag ggatggacat accttcccgt ttttcaattt cctcatccat
     2761 ctcaagaacc ggccggtgaa gcatcctgga aagttcagct gcgatcgtgg actttccgct
     2821 tcccataaaa ccgatcagaa ataaatttcc ggtaaaagat tctgcctgtg tttttgtatt
     2881 tgctgttgtc atataaactc ctttgtactg tacaaaatca attctgacgt ctgcagtttc
     2941 agttggatgt acgccaggat gcacggagat tctgtcaggt aaatgtcagc agagctgacc
     3001 agaattgcgt gccaggtctt acgtgcactc gacttgaaaa aacagacatc acattggcac
     3061 aattataaca cgtaagtgca agtctatcca gtcaaaatca gtggcttgcg gctttttttg
     3121 caaagtctgt ggtcacgagc tgttcataag ggactttttc actcagctca ccggcacttt
     3181 ccagaatgtc agaaagcagc aggaaactgt ctttttcaaa aatcagagaa tctttccagg
     3241 tatcctgttc ctgataacgt ttgacgatgg cggtcagtgt ctccatgtcg gtatcatcaa
     3301 actgcggttt gatcacctgt gcgatttcag caggcgtgtg ggcatgcaca taatccatac
     3361 ctttttgcag agcattggtg aatccctgga tgatctgagg tgattctttc agataactgg
     3421 attttgcaca gtaagctgtg taaggaacat agccggagtc aacgccgagc gaagcaacaa
     3481 catagccgct gccttcctgt tccagagccg tggcagacgg ttcgaactcg acggtgtaat
     3541 caccctgtcc gccggagaaa gcggcagcag tagaaccaaa atcgatactc tggtcaatgg
     3601 aaagatctgt tgccggatca aggttattct ttttcagaat atactcaaaa accatttccg
     3661 gcatgccgcc ttttcgaccg ccaagtactt ttttgccttt taagtcctgc caggaaaaat
     3721 caggatcctc ttctctggcc accagaaagt tgccggcacg ctgagtcagc tgagcaaagt
     3781 tgacaacgga gtctttggct ccttcggcat aggcatagat cgttgcctca gctcccataa
     3841 agccgatgtc tgcctcgcca gaaagtacgg ctgtcatggt tttatctgca ccaaacccgg
     3901 tgacaagatt cagatctatt ccttcttcgg caaaatagcc ttcctgtatg gcaacatatt
     3961 gaggggcata aaaaatggaa tgtgctactt cgttcagggt tactttggaa agcgtttcac
     4021 cggaaggatg ttttttgcag ccgacaagtg cgagcagtgc aaaaaggatc aggaaagata
     4081 agagaaatga tttctttttc ataaaaactc ctttcttttt
//
LOCUS       DAWDWH010000193         4116 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_8323_length_4116_cov_10.299680, whole genome shotgun sequence.
ACCESSION   DAWDWH010000193 DAWDWH010000000
VERSION     DAWDWH010000193.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8323_length_4116_cov_10.299680"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    479..488
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1041 a         1040 c          797 g         1228 t
ORIGIN      
        1 caatcacctt tgtttttatc ttatactgtg gaatttctgt aaaaatcgcc tgcaccgaat
       61 gattaccgga caccgcacgg aacgtgtatt ctgtctgttt cggatcactt tctacctgtt
      121 ctccgtctac gatcagctgt gacagctcat aatgttcatt cggctgaaaa tttacctgat
      181 agtcggttcc gccctccacc gtctcctcac tggcactgat cgttccgttt tttgccgatg
      241 cagtgatgtc ataagctgca cgcagcgttg ccacataaag gcggactctt cttggattgc
      301 taatccccac accaagcata attttgcccg gctcgatctc acagagggat tcgatttcct
      361 ttttctcatc atcgcccgga agatccgcat aaaacgtatt cagatattct ccctgggaaa
      421 gggaatagat aacaaaataa ttatctactt tactctctcc gtcataacac ggcgagatnn
      481 nnnnnnnnat cccctgtcac acagaaatcc tgatacgttg atcctggcgg tttggttctc
      541 ggagcgatga ttttcagaat ctcgaaattc gagttcatca gacggaactt acttccgttt
      601 ccgcctgata ccacgtactg atcggtactt tccagatact cgatcccgcc gaaattctgc
      661 ccgctggagg actgaaaggt ccgtttccag tgcagtgtat ccgcatctac cacataaata
      721 ttgccttcat tttccttatc cagacatggt cccggcatca cgtagatctc attggtctta
      781 ctattgtaag tcatgccgtt tccatgctca taatctcgtt ccgttacttc aaacgcataa
      841 ctgtaatcgt ctttgttaaa cgcgatcagc gtatccggga ttgcttccgc tgctgccccg
      901 ttgatcagac atacaatata cttgtccgta acacaaatag actggggcca tcctcttgct
      961 gaagaagtct ccagctcacc gattgcctcc cagtctgcat agatcagatc tgatgcatca
     1021 taaatatctg catgaactct cagcggatga catacccccc atatcactcc gactatcatg
     1081 cagtaccata tacagaatcg caacattctg cacaggttcc ccttattttt tttcatttct
     1141 cggtaacacc gcctttattc aatcaaatac acgcaaaacc tgatacgtga atttcttttg
     1201 cttcacgagt atagcacatt tttacactca atatcaagta ttctgctttt ctttacaact
     1261 ttctgctttt atgttacact ctgtttaaga tttgggtgaa cgcaatttcc ccaggataac
     1321 gactatatca taaccaaagg agtatactat gtacgattac ctgattgtcg gtgccggact
     1381 gtttggtgcc gtttttgctc atgaagcaaa aaaacatgga aaatcctgcc ttgttgtaga
     1441 aaagcgaaat cacatcgcag gaaatattta ctgtaaaaat gtagacggaa tccatgtcca
     1501 ccagtatggt gcccatattt tccatacttc tgaccgtaaa atctgggatt atatcaacca
     1561 gttttccgag tttaataact acatcaactc accggtagcc atctataaag atgagcttta
     1621 taatcttcct tttaatatga acaccttcag taaaatgtgg aacatccaga caccggctca
     1681 ggcaaaagcc aaaattgccg aacagatcgc agatcttggc atcacagagc caaagaacct
     1741 ggaagaacag gctttatctc tggtcggaac ggatgtctac gaaaagctga tcaaaggata
     1801 cacagaaaaa cagtggggac gctcctgcag ggaactgcct tcctttatca tcaggcgtct
     1861 tccgcttcgt tttacttatg ataataatta tttcaactcc cgctatcagg gcattccggt
     1921 tgaaggctac acagccattg tagaaaaaat gctctccggt gcagatatcc gcttaaatac
     1981 tgactatttt gcccataaag aagaactctc cgccctggca cacaagacgg tatttaccgg
     2041 tatgctggat gaatatttcg gattctgcta cggatctctg gaatatcgtt ccgtacgttt
     2101 tgaggaagaa cgtctggaat gtgacaatta ccagggaaat gccgtggtaa attacaccga
     2161 ccgtgaagtt ccttatacac gcatcatcga acacaaacac tttacattcg gcacacagcc
     2221 attcaccatc atctccagag aatattcttc ggaatggaaa ccgggcatcg aaccatatta
     2281 tccgatcaat gatgccaaaa actccgctct ggcagccaaa tatcaggaac tggctgcaaa
     2341 ggaagagcgt gtgatttttg gcggaagact tggcaattat aaatattacg atatggatca
     2401 ggtcatcgct gccgcactcg actgtgccga tgcagaactt tcctgatatt tacttaataa
     2461 agcagcagtc atcgcttcaa agcaatggct gctgtttttc tgtttctcct taaagaccgg
     2521 aaagatcctg caccagtttg tccattcttt ttgcaaaggt atgcttttcc agcacaagct
     2581 gatggcctct ttcggcgagt ttttctgctt cctttggatg ttccatataa taatccactt
     2641 tttctttcag atctttgcag tctttgtaag tgacaaccgt tccttcaaag atctccgcga
     2701 tctctggcat ctcatcactg atgataaacg cttcggctgc cagtgcatca aacaggcggt
     2761 tggagacaat accggtttct ttcatatcgt cccagtggtc attgagcacg atcttcgcat
     2821 cgcggtatgc ctgaccaacc tgatcgtttg gcatgtacgg ttctctgaca cacttctgtg
     2881 cttcttcaaa tgcttcccag tgtcttccgt agatggtgag cggataatcc agtgtcaggg
     2941 catcctgcac gatctgacgg taaacacgcc tggaattgcc gataaagagc agatcgtatt
     3001 tcttttcgcc ggcagtactc ttcatcacat ccggatctgc acactgcaaa agcacatcgc
     3061 attttgtttt taattcgttt tcccattttc tgcacagttt ttccgatgca aagtagacca
     3121 gatcaaaccg ttcgtattct ccctgtggaa tatcagccgg atgcgaaatg ttccacatga
     3181 tatttttctg tacaatctct tctttcggat aatatggccg gtttccacgc agagcaatcg
     3241 taacggcact gtctgtgctt tcataccatc tctgataagg gcgtacatcc acctgatagc
     3301 cgtgcttctc cagctccttt ttcattgcca aggcataatg ataatccccc cagaactttt
     3361 tgttttcgtc atccggcatc ggtgcacaaa tatcaatctt tttcgaatcc ggcacaatcg
     3421 gcttatcctc ttcctcagaa aacatccgtg tcagacagct ctgccatttt ccctgataaa
     3481 catatagatt ataaagtctt cgatcctttt ctgcctgatc cggtttgctc ttttcttctg
     3541 cactctgaaa agcaagtgca gccggtgcca gaagattctg atatccggct tttgaaagtt
     3601 tcaggcagag gtctgtctct gaaaactcat ctttttttac atggatggac tggtaagccg
     3661 gatcaaaacc acgtacttcc agaaaatcat acggctgcca gtaccaggtt ttgttgcggt
     3721 tttttttgtg gaaggcaatg ccgcagctct tcaccgcagc gaactttgaa atggctttct
     3781 cagattcagg atagagaatc cttgctccca cagccccggc cttctcgttc tgttctgctg
     3841 caagcagcat ggcattcaga cattcggatg ccagctgtac gctttcatcc agaaaaaaca
     3901 gatactcacc cgttgctttt cttgcggctt cgttgcagaa ttcggcaaat gaggcgtttt
     3961 gttttgattc aatgtctgcc gtaaacactt caaactccag gccggctgct gcttctttaa
     4021 aattctcttt cagcttctgc agctgctcct cgcctgtatg atgatgcaca atgacagaaa
     4081 ttttccggct gtttttatcc ggagcgttct gctttt
//
LOCUS       DAWDWH010000194         4100 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_8354_length_4100_cov_17.766625, whole genome shotgun sequence.
ACCESSION   DAWDWH010000194 DAWDWH010000000
VERSION     DAWDWH010000194.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8354_length_4100_cov_17.766625"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2368..2377
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1051 a         1063 c          780 g         1196 t
ORIGIN      
        1 cttgttatta ctcaccactt gcagaagtgg gagtcttctc gactgagata aaaaagtggc
       61 tatctcctac acccctagga gatagccgtg tgtaatttat aaacttcgtt tataagtttc
      121 cggtggcaac catcggacat acgcaaacct tctgattgct tctaagatga ttataaacta
      181 ttcacttcca tttttcaagt gaaaaagttt gaagaggctg ccgttttcag gcagtctctt
      241 tactttcatt ttaaaatccg ctatacttga aatcaattac ttctgccctt tatggcagct
      301 tttatatgga ggtagaaatg ttatgttccg tttatggggg aaaattttta aagacaacca
      361 tatgcttcga gataccgtca tagaagatga ccgaaacgat acacgcacac acaaaatctt
      421 tgattcttta gatcagatct gctacgacct tgatctcggc aaaccgatct ggctcgattc
      481 caatattcag gaattcaaac gccacagcaa aactcgtttt taccaggata gttttataga
      541 agaaatcccc ttcgactacc ttgaacttca ggtaatcgaa gaggattgac aactgtttca
      601 gctatgtgca tcctgtgata tcgaactgtt tggtatcaca ggattttttt gatattttca
      661 tagactccgt ccagcagtct ttcacgagtc tctttcggtg cccaggcaat atgaggcgta
      721 atgatcagct tgccgctgtc ctggatctgc cttagtggat tgtcctccct catcggctct
      781 ccgtccagca catccagagc tgctgccgca atcttatttt cctgcaaagc ccacgtaagt
      841 gcttcctgat ccacgatcgc tccgcgtgcc acattgataa aaattgcgga cttcttcatc
      901 ttctcaaaag ccgctctgtc aaaaagtttt cttgtgtccg ggttcagcgg tgcatgcacc
      961 gatatcagat ccgaacgttt cagcagttct tcaaaatcca ctctctcata cagctgatcg
     1021 gtgttcttcc cggatgttga ataataaata acattgcagc caaattcctg tgcatgggat
     1081 gccacacgtt ttccgatcgc tcccatgccg acaatacccc aggtctttcc gtcaagctct
     1141 gaaaatttct gctcaaaatg agaaaactga gcggaggtac aatacgcacc tgattttaca
     1201 tagtgatcat aataggccag tttatgcaga acatagaaca tcagtgcaaa tgtatgctgt
     1261 gccactgctt ccgtagaata gccggctaca ttcgctgaca ggatgccgtg gctgtctgta
     1321 tatgcccggt caatattgtc cattcccgta gccgttacgg ctaccagttt cagatgcgaa
     1381 gcacctgcca gcgttttttc attcatcgga attttgttga tcacaacaac atctgcatct
     1441 tttacccgct ccggcaccag ttccaccggt gtgtctctgt attttgttac atgtcccagt
     1501 tcgtcaaatc tgtccagatt tatgtcatca ccaagcgata tggtctccag aaatacaatc
     1561 tccatagcag tttcctctct ttcgtaatgc tagtctgcag ggaaaatccc tgctccatta
     1621 tgacaataac tctttcagaa cttcccgtat cacacgttcc tgctcatccg aatccatctg
     1681 ccgcagaatc ttcgccgtct gctctttagg tgtatccgcc gcagctgctg tctcacggat
     1741 accctcccgg atttttccga gattctcctt cagctcctgc ttgtccctga taccatcctt
     1801 gctgttcttt acgttcttct ttttccgtcc cggcatggaa accatttttc cgaaatgtcc
     1861 ggatacgtct tccgactcag cagaaactgc cacttcttct gacgatgcat cgcattttgc
     1921 catttccacc tgatttgccg gttttgaaaa ttccgccagt atcggctgca ccgactccgg
     1981 ctgtttctgt cttgcaacag aagattttgc agcctgtaca ccggaagaag catgaagcac
     2041 cagcacatta tccagctcgt cctgcaaagt aatctccaat gactgttttg caaatcctgt
     2101 atccagaacg ccaaagacca gaagagaaat tccctccatc agcaaagtgc ctgtcagata
     2161 agtctgacgc atgccgccaa ccgcctgcgg ctggctgata taccatccga ccgcccccag
     2221 tgccgcacat aatatggcag ccatctgtgc catccgtttc caggtatgca aagatcctcc
     2281 aaaaagacgg tactccagca gatttttgcg gataaatgct tccgtgttaa aagcattgac
     2341 cgtattctgt ttcattctgc atacgttnnn nnnnnnnctg ataacgctgg cgaagctgtt
     2401 tcataaactt acctttcacc atgccctgcc ttctgacatc tctgagcagg tttccataca
     2461 tcgaacgaag taccacctgg ctgatcactc cccaggcaca gatcaatgcc attgtgtaga
     2521 acaatattgt ctgctgactt aaaaactgta acatggataa tcccccttct gatcctgctt
     2581 gttttcattt cacgtgtcgc tgctgcagca gtcacattct ccggatgaat gttgcaggca
     2641 cattatagcc tcagaatttg catttgaaaa gcagaatcgt tcgacaattt ctgctcttat
     2701 ggctcgaaaa tcgggggatt tttttatgaa gttcttcagt tttcgttctt ttttctacgc
     2761 ttcttttgca gccagattct tcatcatgcc gcaaaccagt ctgacgctgt gagcaactgc
     2821 ctgcttctca aacgtcggat aatccatgga tgcactgtca tctgccttat ctgaaatcgc
     2881 acgcagaaca acaaacggca cttcatttaa gtgtgcagtc tgtgcgatcg cagcaccttc
     2941 catctcggta cagtatcctg cgaaaagtgt cgaaatgttc tctttggttt ctttgtctgc
     3001 gatgaactga tcgccgctga caactcttcc gtgaaatacc tgaatttccg gattaacctc
     3061 ttcacatact ttctcagcca gatccgcaag tgccttgtcc gcctcaaaag agaacacatc
     3121 catctgcgga atctgtccct ttggataacc aaagcttacc gcatccatat cgtgatgaac
     3181 cagatcgctg gaaataacca aatcaccgat gttgatctcc gctttcagag aaccggcgat
     3241 tcctgtattg atcactgctt ttacatcaaa cacatcgatc aggatctgtg tgcagaccgc
     3301 cgcatttacc ttcccaacac cgctgcgtac cacaacaaca tcttgacctt ccagaatgcc
     3361 ctcgcagaat tccatgcctg cttttgtcag ctgacgcgta atctgcatct gctccttcag
     3421 ctcttttacc tcaacttcca ttgctcctat aattccaatt gtacccatgt ctgtctcctt
     3481 ctatattaat gaatttggca cttataaaat ccattcattt ttctgtaatt taagattttt
     3541 attacaacac ttctattttt accgtaaaat caggtctatg tcaagctgag caaaatttca
     3601 tatcagtggg atttttgcag aatacaatat gtcttgcatc cgcctgataa aatgcgtata
     3661 atcaagtcaa agcatcagga aatcttgctc cgtgagatga caggtatttc ctaactcgca
     3721 taaaaaacag gaggtattta aaatgcaggt atataaaaca agaggtgtct gttctcaggc
     3781 aattcagttc gaactggacg gagacaccat caaacatgta aactttatcg gcggatgttc
     3841 cggaaacaca cagggcgttg cacgcctggt agaaggcatg aaagtaaaag atgccatcgc
     3901 cagactggaa ggcatccact gtggtccaag accaacttcc tgcccggatc agctggcaaa
     3961 ggcattaaaa gaagcaatca atgcataatt tccggattat attcttataa tatgatgtgg
     4021 gtgtcaaaag tccccaataa tgattgcaat cgagtaggag gcggtgacta accgccgtcc
     4081 tctcacacca ccgtgcgtac
//
LOCUS       DAWDWH010000195         4092 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_8371_length_4092_cov_10.429775, whole genome shotgun sequence.
ACCESSION   DAWDWH010000195 DAWDWH010000000
VERSION     DAWDWH010000195.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8371_length_4092_cov_10.429775"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2315..2324
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1254 a          732 c         1096 g         1000 t
ORIGIN      
        1 tatgattcgg cgggattggc tgttcgagac ggagagaatc tggcacaggt tgtgaaggca
       61 aagggacgct tgagcaatct ggcggagaaa acggacgatg gtcatacctt aaaaggtact
      121 tgcggtatcg gtcatactcg ctgggcaacg catggggaac cgagtcagac aaatgcacat
      181 ccgcatgtgt cgggaaactg cagcggttct ggctcaggta tggtagaatc cgaggttgtt
      241 ggtgtgcata atggcattat tgagaattat acggagctga aggaaaagtt attaaaacat
      301 ggctatactt tttacagtca gacagataca gaagttgtga tcaagctggt ggactactat
      361 tataagaaat ataatctggg tccgatcgat gcgatcgcga aaacgatggt gcgtgtacgc
      421 ggttcgtatg ctctggaact gatgtttaag gattatccgg gggagatctg ggtggcacga
      481 aaagacagtc cgatgatcat tggtattgca gacggcgaaa cttatgttgc ttctgatgtt
      541 ccggcaattc tcaaatatac cagaaatgtt tattatattg gaaatctgga gttcgcaaaa
      601 ctgactccgg gagaggcaca tttttataat cttgatggcg atgagattga aaaagaaaca
      661 accgagatca agtgggatgc cgaagcagcg gagaaagccg gttttgagca ttttatgatg
      721 aaggagatcc atgagcagcc aaaagcaatc caggatacgc tcaattcggt catcaaagaa
      781 gataaaattg acctttcaac agtagaaatc acggacgaag agattcagaa ttttgatcag
      841 atctatattg ttgcctgcgg atcggcatgg catgtgggta tggcagcaca gtatgtattt
      901 gaagatctgg cggatattcc ggtgagggta gagctggcat ccgaattccg ttatcgaaaa
      961 atgccattaa accagaaagc actggtgatc gtagtcagcc agtccggtga aacagccgat
     1021 acactggcgg ctcttagaat ggcaaaagaa aagaatatta ccacaatggc gatcgtcaat
     1081 gtagttggaa gcagcattgc acgcgaagcc gataaggtat tctatacact tgcaggcccg
     1141 gaaatctcag ttgcgacaac gaaagcctac agtgcacagt tagccgcaat gtactgcctg
     1201 gcagtgcagt tcgcaaaagt cagaggaaag atttcagaag aacagtacag ctattacatt
     1261 tctgagatgc ttaccatccc ggataaaatg cagaaagtcc tggaagataa agaacggatc
     1321 cagtggtttg cctccaaata tgcaaacgca catgatgtat tcttcgtggg ccgcggcatt
     1381 gattatgcag tctgcctgga aggaagctta aaactcaaag aaatttccta tattcattct
     1441 gaagcctacg cggcaggaga actgaagcat ggaaccatca gcctgatcga acagggaacc
     1501 ctggtgatcg gagtcctgac acagagcgaa ctctatgaga aaaccatcag caatatgctc
     1561 gaatgtaaga gtcgcggagc ttacctgatg ggtctgacaa cctatggaaa atacgaaatc
     1621 gaagatcagg tgaactttac cgtttatgta ccaaaagtag atgagcattt tgtcggaagt
     1681 ctggccgtgg ttccactgca gctgatcgga tactatgtca gcgtggcaaa aggactggat
     1741 gtggataaac cgagaaatct ggcgaagagc gtgacggtgg agtgatatca ggtgcggtat
     1801 gacctgcaaa gaaggcgcag cagcggtgaa agatttcaga gaacgtaaac aatactatat
     1861 atcaaggagg tgcagcactg catgagtaat gtattacctg ttgaggattt aagtaaaact
     1921 tacctggaac actctatggt tatcaacaat tttgttatca aaatcggcag tcagattaaa
     1981 gacagtctgt gtcgtgtctt tggtgatggt gtgcagtatg aatggcgtga gaacgatgat
     2041 aaagtaatga ttccggatgt atccattatc tgcaacttga gagatcgaaa gaatatttct
     2101 tttacaggta ttcctcgttt tgtgatggag gtcttatcca atgctacaga gaaatacgac
     2161 aggcacgaaa aaatgaatat ttactgcaaa gtcggagttt ctgaatactg gatcgttgat
     2221 tggagaaaaa agtccgtaga aatctatctt tttgacttcg aagaggatgg aacaggatat
     2281 ccgtatcttt ataaaacagt tactgcacag aatannnnnn nnnnaggagt tacaactggt
     2341 gatgttcccg aatttgaaaa taacctttga ggaattgttt gatcttggag aatattgatt
     2401 ttacatgact ctccggtgca gtcagggaat gatttcagct tttcggtttg acttagtaaa
     2461 tgctatgagt tatgatactt aaaagaagta acttgtgagc cagtcgctca tgagcataat
     2521 tagtggagga aatagcatga aattttttgt aacaggtgtc ggcggacagc ttggacatga
     2581 tgtaatgaac gaactgatca ggagagggca cgagggtgtc ggttctgata ttcaggagca
     2641 gtacagcggt gtggaagatg gttctgaggt gacaaaagca ccttatcttt cactggatat
     2701 cacagataaa gaagcagtaa agtgtgtcat tgagagcgta aaaccggatg cggtgatcca
     2761 ctgtgcggca tggactgcgg ttgatatggc ggaagatgat gacaagattg caaaggtccg
     2821 tgcgatcaat gcgggcggca cacagaacat tgcggatgtc tgcaagaagc tggactgcaa
     2881 gatgacttac atcagcacag attatgtatt tgacggtcag ggcacagagc catggaaacc
     2941 ggactgcaag gattataagc cgatgaatgt ctatggacag acgaagctgg aaggggaact
     3001 ggcagtaagc cagacactgg agaaatattt tatcgtccgc attgcgtggg tctttggctt
     3061 aaatggcaag aattttatca agacgatgct gaatgtcggc aagacgcatg acacggttcg
     3121 tgtggtcaat gatcagatcg gtacaccaac ttacacttat gatctggcac gtctgctgat
     3181 tgatatgaat gaaacagaaa aatatggtta ttatcatgca acgaatgagg gcggatacat
     3241 cagctggtat gattttacga aagaaatcta ccgtcaggca ggttatacga ccaaggttct
     3301 gccggtaacc acagcagaat atggcttaag caaagcggca agaccgttta acagccgtct
     3361 ggataagagc aagctggtgg aaaatggatt taccccgctt ccgacctggc aggacgcact
     3421 gagccgttat ctgaaagaaa tcaaagtgtg atttcggata cattttaaat gataagtaca
     3481 aaaaataaga agtgggagtc ttctccgact aagataaaaa gatactgaac agttgcagaa
     3541 ggagataaaa aaaatgggac agatcaaagt tgagaaaaat gtaggcggca tcgaaggcct
     3601 ttgtattgta gaaccggccg ttcatggtga cgagcgtggt tatttcatgg aaacatacaa
     3661 cgaaagagat atgaaagagg caggcattga tattcatttc gtacaggata accagtccat
     3721 gtccacaaaa ggagtattga gaggcctgca tttccagaaa caatatccgc agtgcaagct
     3781 ggttcgtgcg gtacgcggaa cggtatttga tgttgccgtg gatctgcgtt ccgattccag
     3841 gacttatgga aaatggttcg gcgtggtttt atctgcggaa aataaaaagc agttcctcat
     3901 tccggaaggt tttgcacatg gatttctggt gctgagcgat gaggcagaat tctgctataa
     3961 agtgaacgat ttctggcatc caaacgatga gggcggcatg gcatggaacg acccggagat
     4021 cggcatcgag tggccggaag tgaagggcga gtacaaaggc agtgcatcgg cagaaggtta
     4081 tacgctggca ga
//
LOCUS       DAWDWH010000196         4008 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_8563_length_4008_cov_10.237794, whole genome shotgun sequence.
ACCESSION   DAWDWH010000196 DAWDWH010000000
VERSION     DAWDWH010000196.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8563_length_4008_cov_10.237794"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    968..977
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          910 a         1126 c          814 g         1148 t
ORIGIN      
        1 ggtgatatat gatccctcgc tgccactgcg attggaaaat cgccggcctt gtaaggatta
       61 tcacccaatg taacttccgc acatacggtc tcgtaagcca gtgcctcgta attattctcc
      121 ctgcttaaat gtccaagcag aatggttttg atcctgtcat ttaaaatctc acagagcaga
      181 cggcctgcat tttcatttga caggtgccct ctgttcccga ggatcctctg ttttagatag
      241 tagggatagc tgccaacctg cagcatatgt atatcgtggt tggattcgag cagcacgccg
      301 tcaagatctt tcagatgaga aacaatcttc tcatcgtaat aaccaagatc cgtcacaacg
      361 gcaaccgatt tctctccatt gtccagacga taggcaaccg gctcggcggc atcgtgcgat
      421 atggcaaacg gttccacctc cagatcttcg atctgaaatt tcccatctgc ccggatcgta
      481 tggaacaaat cttcatcaat ctgaccaagg ctgctactgt ttctgatccc gtcgatcgta
      541 cctgctgtcg catagattgg gatatgatac tttctggaca tcacgcccag ccctttgatg
      601 tgatccgaat gttcgtgtgt gatcaggatc cccttcagct cttccggctt ccggtcgatc
      661 tcatgcagtc cgtccacgat cctttttctg ctgatcccgg tatcgatcag cacagaagtc
      721 tgatcagaac ccacataaat gcagtttccg ctgcttccac ttgctatact acaaaaatcc
      781 atgttctttt aatcccctgt ccatttgttc aaatgtattg tctacaaaat cactgctgtt
      841 gtcaaccaca aattgacagg actttcgaaa tacgctgtct tttttctgat ttttcaaaat
      901 atcatctatt ttgcggtcac tgtaaccgcg gctctgtttc agacgtatgc gacgtgccgc
      961 atcgtccnnn nnnnnnngtc cacataaata taccagatct catcacagat ctcgtcgtaa
     1021 tgttcttcca gcaaaagagc tgcctccacg accacaaacg gcacacatgc cttttgtttt
     1081 tccagataaa cggcagttcg aatgtattct ttcactgccg gatgaacgat cgcatttaac
     1141 atggcaagct tttcctgatc tgcaaacacg atcgcaccca gttttttccg gtcgatcttc
     1201 tgatctgccg ccaggatctg ctctccaaac aaagaacaga tctgcttata gcaggaagta
     1261 tccggctcca tcaccagatg gccgacctga tcagcctgga tgacttttgc cctgtatttc
     1321 tcctccagat aggcaaggat cgtacttttc cctgccccta cctgtccggt aatcccaagt
     1381 actttcatat atttataacc tgctttctgt actttttgca atctccggtc tattttgcct
     1441 cgtaccagtt ctctccggta tgcatatcga tctccaggca aacttttaaa ttcgccgcac
     1501 catgcatctc ctctgcaaga atctgtttta ccatatccag ctcctctttt gccgcctcca
     1561 caagcagttc atcgtgcacc tgaaggatca gtctggatct gaaatgttct gctttcagcc
     1621 gctcattgac acggatcatg gcaatcttga tgatatctgc cgccgtaccc tggatcggtg
     1681 aattcatcgc cacacgctcc ccgaaagaac gctgcataaa atttgaagaa gaaagctccg
     1741 gaaccggtct tcttctgcca aacagtgtcg tgacatagcc tttttctttt gcatccgcca
     1801 cactctgatc caggaactgt ttgatgcccg gatacgtctc aaaatatttc tcaatatatt
     1861 ctgcggcttc ttttctggag atgctcaagt cctgacttaa gccaaaggag ctgatgccgt
     1921 agacaatgcc gaaattaact gccttggcat ttcttctctg caaaggtgtc acttcatcaa
     1981 acggcacatg gaacacctgg gaggccgtga tccggtggat gtcctgagct tcccggtaag
     2041 cttcaatcag cctttcatcc ccggaaatgt gtgccagcac acgcagctcg atctgggagt
     2101 agtcggcatc cacaaagaca aatccatctt ccgggataaa gactttgcgg atcattctgc
     2161 caagttccat acggatcggg atgttctgaa ggttcggctc cgtgctgctg atgcgtcccg
     2221 ttgccgtgat cgtctgatgg aacttgctgt gaatccttcc gtctgccgca atgtaacctg
     2281 ccagtccgtc cgcgtaggtg gatttgagct ttgcaagctg acggtactcc agaatatggg
     2341 acacgatcgg tgcctccggt gccagtttct ccagcacgtc ggccgcggtc gagtagccgg
     2401 tcttcgtttt ctttccatat ggaagctgca gtttttcaaa caaaatcaca cctagctgct
     2461 tcggagaatt gatattgaag gtctctccag ccagatcgta gatcgtcgtt tccagttctt
     2521 tgatccgtcc ggacaactgc tccccatact gctttaacgc ctctccatct gcctttatac
     2581 cggctttttc catgtcgtaa agcgtataga ccagcggcat ctcaatctcc ttaaacagat
     2641 cccacatacc gttctctttt aacttttctt ccagttttgc ttttccttcc aagcaggtgt
     2701 acgtgccgta gcaggctgca tttttcactg ctttctcatc ttccgatcca aaatcttctt
     2761 ttccaagcag ctcctgcacg gacggaacca gaagatccag acattctttt gcaatgtcct
     2821 cgtacgcata ggtatctttg agtggattta aaagatacat ggcgatcgct gcatcaaaga
     2881 gatttttctt ctccacgtgc agatacgaaa gctgctcttt gacgttcatc gtgacagccg
     2941 catccgcttt ttccagcact tccgtcattt tttcacagag atactgaccg gtcacaaaac
     3001 cctgtgccgg tatataataa atcttttctt tctcccagcc aagagaaata ccttttactt
     3061 ctcctttgtg agaaatgaca gaaaagctca cgagtgctgc ccctgctgcc tgtgcaaaca
     3121 cgtcttccgc ctcaccaaaa tcttccactg cgtaaaaata ctgttctatc ttctgatccg
     3181 gtgcatcaca ctgaaatctt cccagcagat tcttaaactc cagctttttg aacagggcaa
     3241 aggcttccgg cgtatacaga tcggtaagtc tcgcatcctc cagcacaaaa tcaatcgggc
     3301 tgtcggtatc gatcgtcgcc agttttttgc tgaggactgc cagatcatag tgctccagaa
     3361 aagcattttt cgccttggtc ggcttgatct cctccacatg ggcacaggca ttctcgatcg
     3421 tgccatacgc cacgaccagt ttggtcgccg tcttttcacc gactcctgga agtcccggaa
     3481 tattgtccga ggcatcaccc atcatcgcct tcagttcaat gatctgcggc ggtgtcacct
     3541 gatacttctc cagcacttct tttgcatggt aatcctcgat ctccgtgccg gtccgcttcg
     3601 tctttggtat gcggatcatg atgtgctctg ttgcaagctg caaaagatcc cggtcaccgg
     3661 agaccacaga aacatccaga ccttcctttt ccatccgctt tgccaccgtt ccaagcaggt
     3721 catccgcttc atagccttct ttcatcagaa gcggtacgcc catcgccgtc agcacttctt
     3781 tcatgaccgg cacctgctcg tggagctgcg gcggctgcgg ctttctcgtc cctttgtaag
     3841 catcatacat tttatggcga aaggtcggtg ctttcaggtc aaaagcaacc gccagatact
     3901 gcggctgttc ttccgcaagg attttaaata aaatatttaa aaatccatag accgcattgg
     3961 tatgcagccc ttccgagttg gtcagctccg gcactccgta aaatgccc
//
LOCUS       DAWDWH010000197         3975 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_8627_length_3975_cov_14.749490, whole genome shotgun sequence.
ACCESSION   DAWDWH010000197 DAWDWH010000000
VERSION     DAWDWH010000197.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8627_length_3975_cov_14.749490"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2790..2799
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1174 a          852 c         1027 g          912 t
ORIGIN      
        1 tcttctccaa aacctgtgag tccctcggca tctttttagg ttctgagaga gaatacggaa
       61 tcggtatgtt cttcttccct caggacgaac tgaaaaagaa ccaggcaaag aaaatgtttg
      121 agatcatcgt agaaaaggaa ggtcttgaat ttttgggatg gagggaagtt ccggttgttc
      181 cggaagttct cggacacaaa gcactggaat gtatgccatg tatcatgcag ggatttgtag
      241 caaaaccaaa acacctggca aaaggcattg attttgaccg cagactttat gtggtaagac
      301 gtgtgtttga acagagcaat gacaattcct atgtagtttc tctttcaagc agaacgatcg
      361 tatacaaagg tatgttcctg gtaggacagc tccgtacctt cttccgtgat ttgcaggatg
      421 cagattatga atctgcgatc gcaatcgtac actcccgttt cagtaccaac acaaatccga
      481 gctgggagag agcacatccg aaccgcttta tcgtacacaa cggtgagatc aacacgatcc
      541 gtggaaatgc cgacaagatg cttgcacgcg aagagacgat gttctccgag catttcaaag
      601 gagagctgca caaagttctg ccggttgtca atacttccgg ttctgactcg gcaatgctcg
      661 acaacacact ggaatttatg gtgatgagtg gtatggatct gccgctggcc gtgatgatca
      721 cgatcccgga accatgggca aacaacaaga ccatgagcca gagcaaaaaa gatttttatc
      781 agtatcatgc aacgatgatg gagccgtggg acggaccggc atcgatcctg ttcagcgacg
      841 gagatgtggt aggagccgtg cttgaccgta acggattgcg tccttccaga tactatatca
      901 ccgatgacgg ttatctgatc ctctcttctg aggtcggtgt gctggatatc gatccgacca
      961 agatcgtgct caaagaaaga ctgcatccgg gcaaaatgct gctggtggat actgtacagg
     1021 gaagagtgat cgatgacgat gaactgaaag aaagttatgc aaaaaagcag ccatatggtg
     1081 agtggctgga tcgttacctg gtaaatcttt ctgatctgaa gattccgaat aaacgcgtgg
     1141 aagagtacag cgatgaggag cgtgccaaac tccaaaaagc ctttggctat acttacgaag
     1201 agtaccgtac ttctatttta aatatggcaa agaacggtgc agagggcatc gcttccatgg
     1261 gtattgacac accactggct gttttatcgg aatgtcatgt gccgctgttt aattatttca
     1321 aacagctgtt tgcacaggta accaacccgc caatcgatgc aatccgtgaa gaagtggtaa
     1381 cttctaccac catctacatc ggtgaggacg gaaatctgct tcaggaagag gcaaagaact
     1441 gtcaggtact gaagatcaac aatccgattc tgacaaatac cgacatgctg aagattaaaa
     1501 atctggatgt cgaaggcttt aaagtagcgg aaattccgat cacgtattat aagaacacaa
     1561 gcctggaaaa agccattgac tatctgtttg tcgaagtaga cagagcacac agagacggag
     1621 caaacatcct gatcctgaca gacagaggcg ttgatgaaaa ccgtgtgccg atcccttcac
     1681 tgctggcagt atccgctgtg catcagcacc ttgtaaagac gaaaaagaga acttcacttg
     1741 caatcgttct ggaatccggc gaaccaagag aagtacatca ttttgctaca ctgcttggtt
     1801 atggtgcaag tgcgatcaac ccatatctgg cactggatac gatccatcag ctgatcgaca
     1861 gcaagatgct ggataaggat tattatgcag ctgtggaaga ttacaatcag gctgtcctta
     1921 gcggaatcgt aaagattgct tccaaaatgg gtatttccac aatccagtct tatcagggat
     1981 ctcagatttt tgaggcaatc ggcattgata aagaagtgat cgataaatat ttcacaaata
     2041 cggtcagccg tgttggtggt atcacaatga aagatattgc agaagaaacg gacaaactgc
     2101 attccggagc atttgatccg ctgggactgg atgtagatga aacactgtac agcatcggac
     2161 ggcacaaaat gagaagtgcc ggagaacatc accgttacaa tccgcagacc attcatctgc
     2221 tgcagcagtc gacctggaga ggcgattaca acctgttcaa acagtacacg aatttggtag
     2281 acaaagaaga gaccggttat ttgcgaagcc tgatggattt tgattatccg gaaaaaggtg
     2341 tgccgatcga gaaagtagag agtgtcgaat ccattgtaaa acgatttaag accggagcga
     2401 tgtcctacgg ttccatttct caggaagcac atgaaacact agcgatcgcc atgaaccgtc
     2461 tgcatggtaa gtcaaacagc ggtgaaggag gcgaagatct ggagcgtatg atcgttaccg
     2521 atccgaacag gaaccgttgt tctgcaatca aacaggtagc atccggacgt tttggagtta
     2581 cctccaaata tctggtcagt gcacaggaga tccagatcaa aatggcacag ggagcaaaac
     2641 ctggtgaagg cggtcatctg ccgggcggaa aagtttatcc ttggatcgca aagaccagac
     2701 attccacacc gggcgtgagc ctgatttcgc cgccgcctca tcacgatatt tattcaatcg
     2761 aggatctggc agagctgatc tacgatctgn nnnnnnnnnt tgcaaaagca ggagcacagg
     2821 tcgtgctgat ttccggttat gacggaggta ccggagctgc accgagaagt tccatccaca
     2881 atgccggact tccgtgggag ctcggacttg cagaaaccca tcagacgctg atcatgaacg
     2941 gacttcgaaa caaagtccgc attgagacag acggaaaact gatgagcgga cgtgacgtag
     3001 cgatcgccgc actgcttgga gcagaagaat tcggtttcgc aaccgcaccg ctgattacaa
     3061 tgggatgtgt gatgatgcgt gtctgcaacc tggacacctg tccggtcggc gtggcaacac
     3121 agaatccgga gctgagaaaa cgtttccgcg gcaaaccgga atacgtggaa aacttcatgc
     3181 ggttcattgc acaggaactg cgtgaataca tggcaaaact tggattctgc accgtagacg
     3241 agatggttgg acacagcgaa ctgctgaaga agaaagaaca tgctgcaaat gtaaatgcag
     3301 cgaaagtgga tttaagccgc attctggata acccgtatgc aggcaaagag atgaaagtga
     3361 cattcgatcc ttctcaggta tatgatttca aactggaaga gacggtggat cagaccattt
     3421 taatgaagaa actgaaagct tctctggaga aaaaacagaa acggagcatc gaagtcgatg
     3481 ttaccaacat caaccgtgct ttcggcacac agtttggtgc tgagatcaca aaacgttacg
     3541 gtgatacact ggatgctgat acctacattg taaaatgtaa aggtgcaggc ggacagagtt
     3601 ttggagcttt tatcccgaaa ggactgacac tggagctggt cggtgacagc aatgactatt
     3661 tcggaaaagg tttatccggc ggaaagctga tcgtatatcc gccaaccggt gtaaaattca
     3721 aagaagatga aaacatcatt atcggaaacg tggcactgta cggtgcaaca agcggagaag
     3781 cctacatcaa cggtgttgcc ggagaacgtt tctgcgtgcg aaattccggt gcaaaagctg
     3841 tagtagaagg cgtcggagat cacggatgtg aatatatgac gggcggtcgt gttgttgtca
     3901 tcggaaaagt cggcaagaac tttgcagccg gtatgagcgg cggtgtcgcc tatgttctgg
     3961 atgaaaacaa cgatc
//
LOCUS       DAWDWH010000198         3961 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_8658_length_3961_cov_10.522273, whole genome shotgun sequence.
ACCESSION   DAWDWH010000198 DAWDWH010000000
VERSION     DAWDWH010000198.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8658_length_3961_cov_10.522273"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    874..883
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1223 a          690 c          949 g         1089 t
ORIGIN      
        1 attagataga ttcttaaatg ccatgaaact gaatgacaat gatggatttg atgacgatga
       61 ctttctggat gacgaaatgg atgatttcga agaagaagct ccaaaaaaga gattcttcaa
      121 aaagcagaat gaagaagacg atttaggtga tctggatatg gatgagaaag tgccttccgg
      181 aaaagaaaag agcaaggcaa aatctttcca gaaatccgga agcacttctt ctcctcgtcc
      241 gtacagtaca tcatctgcaa agaccagcaa ctccggtaag atttctcaga tgcgtacgac
      301 aaaaaaatcc gttgccggaa tggaagtatg tgtaatcaaa ccgcgttcta tggaagacac
      361 aagagagatt accgatacac tgcttgcaaa ttgcacagtt gtactgaata tggaaggtct
      421 tgatgttgat attgcacaga gaatcattga tttttcatct ggttcctgct atgcaatcga
      481 cggtaatctt cagaaagtca gcaattttat ttttatcatt acgccatccg gtgtggaagt
      541 ttccggagat tttcaggaaa ttttgaatgg tgttgatatg ccgtctcttg gaaacaagtt
      601 ttaggagaaa gtaaatggat aagatggagg aacgcacaag gcagcgtatc ctggatctgg
      661 caaatctggc agatcgaaga ggaatcatca cttatacaga ttttatgaat ctgaatgaat
      721 taaatatttt tcacagcacg gtcggaaaga ttccatttgt tcgatggaaa cttttcggcg
      781 gttatgcaaa agcagagcgt cagttggcag cgtttatccc tgatgctctt tattttcgtt
      841 atgatttttc gcaaaagggc gattcagatg aagnnnnnnn nnnaaaagag aatgatctga
      901 tatcagccag aggtatggga agatttgtat acgaaggtat gctttcacag accaaaaagg
      961 gacgttacct tgtaaaactt gaaaaatatg tttaaacagc tcagcgattc tggaaaatcg
     1021 gattccgcat gtattcgatg aatggaacag gaggtaaaaa caatggatac tacaaaatac
     1081 cgcacacaga taccggttaa aaaagatggg atttttgcct atgaaattta tcttgaaaaa
     1141 gattttggga tgcttgcaga gcgtttgaag gtttgtaagc cggaaaaccg aaggttatgt
     1201 attgttaccg attctaccgt ggatcaatat tacggagatc aggtagaaga aattcttgca
     1261 tcctgtgcaa aggaagttac ccgctttgtt tttcgggcag gagaagaaaa caaaaatctc
     1321 aatactgtgc aggatttgta tgagcatctg atcctgcatc attttgaacg aaaagatatg
     1381 ctggttgctc ttggaggagg cgttgttggg gatctggccg gtttcactgc agcgacttat
     1441 cttcgaggcg ttgattttgt tcaaattccg acaacactgc tctcacaggt ggacagcagc
     1501 attggcggta agacaggcgt ggactttaac tgttataaaa atatggtcgg agcctttcat
     1561 cagccaaaac tggtgtatat gaatctgtct gccctgaaga cgctgacaga cgaacagttt
     1621 gcctgtggca tgggagaaat cttaaaacat ggcctgatca aagatcaggg atattatgag
     1681 tggacgatct gtcacagaca ggaaataatg gaacgcagtt tggaagttct ggaagaaatg
     1741 atctccagaa gctgtgagat caaacgtgat gtagtagaga aagatccgac agaaaaggga
     1801 gatcgtgcac ttttgaattt tggacataca cttggacatg ccattgaaaa gttaaaaaac
     1861 ttctctttga tgcatggaca atgtgtagca cttggaagtc tggcggcttc ctatctttcc
     1921 atgaggcgtg gacagataac agaagcagag ctgactgcaa tcaaagaagc gaatatcgct
     1981 tttggtcttc cggtatctgt tgaaggagtg acaccggaag aggttgtggc aacaacacgt
     2041 tctgataaaa aaatggaagc cggaaaagta aaatttattt tgttaaacag tattggagat
     2101 gcctgtattg acagaagtgt taccgatcag gagatggaag atgcggtaaa atatcttgaa
     2161 aatattacgc ttctgtaaca gagaaaggtt aagattatga aaaaaggata taggactatt
     2221 gttgcagttg tctcgctgtt tctgcttgtt cttatagacc aggtcacaaa atttgctgcg
     2281 gtaaaatgtt taaaaggaaa agaagcattt tcgctgattc cgggtgtttt tgaactgcag
     2341 tatcttgaaa acagaggagc ggcttttgga gcactgcaaa atcagagatg gttctttata
     2401 ctgattgtta tttttgtgct cgcagtactt ttatggtgct tcatccgttt tccgaaggaa
     2461 gcacattacc gtccgcttgc agtgctggct gtttttattg cggcaggtgc gatcggaaat
     2521 atgatcgacc ggttgcttaa cggctatgtc attgattttc tgtatttttc gctgattgat
     2581 tttccaatct tcaatgttgc agatatctat gtgacggtcg ctatggttgt attgtttata
     2641 ttgatactga caatttataa ggatgatgat tttcattttc tgaaactgca cggatcagaa
     2701 aaagcgtaaa tcagattgca ggagaaacta tgcaggaact gatattatta ccggaagaag
     2761 aatcgattgg tgagcgtgca gataaatatc tggcagttca gcttccgcag tattcaagga
     2821 gcttctttca gaaacttttc aaggacgatc agatacagat tcaaaacacg ccgatcaagg
     2881 cgagttaccg cattaccgga gaagaagaaa tcgcacttac aatcccggat gcccagaagc
     2941 cggatattgt tccggaaaac attccgctgg atattctgta tgaagatcag gatctccttg
     3001 tggtaaacaa gccgaaaggc atggtggtac atccggctca tggtcattac agcggaacac
     3061 tggtcaatgc actgatgtat cattgtaagg acagtctttc cggaatcaat ggggtattgc
     3121 gtcccggtat tgtgcatcga attgatatgg ataccacagg ctctttgatc gtctgcaaaa
     3181 cggatatggc acacaaagtg attgcagcac aattaaaaga acattctatc catagatgtt
     3241 accgtgccat tgtacatggt gtgattaaag aagattccgg tgtgatcgat gcaccgatcg
     3301 gaagaaatcc caccgatcga aagaagatgg cgatcaatga gcagaatgga aaacgggcgg
     3361 tgacgcattt taaggtatta aaacgtttcc gacagttcac gtatattgaa tgtgagctgg
     3421 aaaccggccg tacacatcag attcgtgttc atatggcttc tctacagcat ccgcttctcg
     3481 gagattcggt atacggtccg gcaaaatcac cttataaact gcaaggacag acacttcatg
     3541 cgatgaccat cgggtttatt catccgtcta ccggaggata tatggagttt tcagctccgt
     3601 tgccggcata ttttgaggag ttgcttcata aacttccata aagtacagaa aatgcagaaa
     3661 ttgtttgtaa gattcaaaaa atgcttgtaa attgtctact caatgatgta taatcactct
     3721 tataaggaga agaaatacgg cagattttat ctgccagaaa gaaagggtgg ttctatatga
     3781 catgtaaatc aattattacg atcggacgac agtatggaag cggcggacat gagattggcc
     3841 agaaactggc aaaagaactt ggaataaaat gttatgataa ggaacttctc gaccgtgctg
     3901 caaaagagag cggaatctgc caggaattgt ttgagcatca cgatgaaagg ccgacgaaca
     3961 g
//
LOCUS       DAWDWH010000199         3921 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_8736_length_3921_cov_9.281686, whole genome shotgun sequence.
ACCESSION   DAWDWH010000199 DAWDWH010000000
VERSION     DAWDWH010000199.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8736_length_3921_cov_9.281686"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1192..1201
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1138 a          858 c          924 g          991 t
ORIGIN      
        1 aacgcggaga aaatgaggcg tgcaaagatc tttatatttc catggtccgt gatatgcaga
       61 aaaaatattt ctttgaaaaa cagtaattct acatcctaaa aaaaaggcgg aactttttta
      121 agagttccgc ttttgctgat tcaaaagaaa aggaggtgac ctatgtacaa cgtattaaca
      181 gccataaaaa acaatatgct tctgcaggaa ctgaaaaggc ttcacatctg gggtgcgtct
      241 accggttttg agatctgtga ggtcacttct gattttgcga cactgacaga gcacctgcgt
      301 aaaaacaggt atcaactggt tttgctggaa gcaacaccgg attacccggt tttaaacctt
      361 ctgcagacca tccggcagga aaagctctgt caggcaatcg cgatggtcgg gcagacggcg
      421 gaatttaaga tcgtgcgaaa aagttttctg ctcggtgtcg atgactatct gatcacgccg
      481 tttgagatca gtcagtttat ctcgctgttt ggcaagatcg aacatgcgga gcatggaaag
      541 attgaggcag aaaacagcca gaaagaatcc ctgcttgact gcttccggac gattgatttt
      601 tccataaaaa aagacctcca caaacttttg taccctgctc gttcagaata ttcagatccc
      661 tcagaatctt tttcttacct gaaacggatt ttggatgacg tcactttgga acttttcagg
      721 cgacatccgt ggctggagct ttattttcag gcggatgatt ttattcttcc ggaaacagat
      781 tttccggact gtgaagaaca gatccagaaa agcgtcgatg atttttattc cctttttaca
      841 gagtttgcgg aactgtatcc tgcacacagt gagggaatgg acaagatttt gttgtatatc
      901 ctgaaccgcc cggaagaaga cctgaagcag aaaacaatat cagaagaact ttacataaac
      961 cgttcctatt tcagtacgat gtttacggct cagatgcaga ttaattttgt tgattacgtc
     1021 aatattgtga aaatgaaacg tgccgcatac cttttaaaac acacaaagcg gaaagtcatt
     1081 gacatcgccg gggcactgga ttacaaggat atggggtatt ttctgaaaaa attcaaggca
     1141 aaatatgggg tgacaccatc ccagtatcga atcccggaga cgtatgaatt tnnnnnnnnn
     1201 nttcaaatat aacattcagt ttctgttatt tttcttacaa aaagcagaga aaggcggtcg
     1261 aaactatggc aaatatctat caggaaatta ccgatatcgt cggaaaaaca ccacttctaa
     1321 aacttcagag atatatggac gcaaatgcct gtaaggcaga tattcttgca aaacttgaat
     1381 atcagaatcc aggcggttcc gttaaagacc gcgttgcgat ccgcatgatc gatgaagcgg
     1441 aaaaagccgg tatcctgcat aaaggcgatg tgatcatcga accgacttcc ggaaataccg
     1501 gaatcggact ggcatgcgtg gcagccgcca gaggctatca tctgatcatc acgatgccgg
     1561 aaaccatgag cgtggaacga agaagactgt tatctgcata tggggcagag cttgtcctga
     1621 caccggcatc caaaggtgtg aaaggttcga tccgcaaggc aaatgaactg gcagaacagc
     1681 tgcacgcttt tgttccgagc cagttcagca atccctataa cccaaaatct cactacgaga
     1741 caaccggccc ggaaatctgg gcagatacgg acggaaagat tgatgttttt gtagctggcg
     1801 tcggaaccgg cggtacgatc agcggaaccg gcaggtatct gaaagaaaaa aatccaaaca
     1861 tccagatcgt ggctgttgag cctgccggtt ccccggtact ctctgacggc atcccgggac
     1921 ggcacaagat ccagggtatc ggtgccgggt tcatcccgga aacactggat atggagatct
     1981 atgatgagat cctgacggta accgatgacg atgcctacca ggcatgccgg gaactcgcaa
     2041 gaagagaagg cgtactttgc ggtatctctt ccggtgctgc attatctgct gcaaaagagc
     2101 tggcagtaaa gcctgcgtat caggggaaaa caatcgttgc cctgtttccg gacggaggtg
     2161 aacgctatct ttccagcggc gtttttgatg aataaaatat ggataagata aaagtgcttt
     2221 tggccatgac gttcttatcc aatggaagaa gtggaacttt ccgaggaacg tttgaagaac
     2281 tgaaacaggt ctgttcttaa aaatccgtat aaattttctc ttttccggtt atgctgtcag
     2341 cagaatgcca aagcttgcat atcaggttct gtatcataca aaatgacaga cctggatcag
     2401 aaaggggata cactatggcg gaaaaagaaa acagcaaaag cgaaaacaca gaaactaaaa
     2461 aaaatgagca gatcagagaa tacggtcaga ccaaactgga acacaacatt cacctgcttt
     2521 ctgtgatcgg agagatcgaa ggacacgaat gcctttcctc caatacaaaa actacaaagt
     2581 acgagcacgt ccttcctcag cttgctgcca ttgaggacag cagtgatatc cagggactgc
     2641 tcgttctttt gaatactgta ggcggcgatg tggaagccgg tcttgccatc gcagaaatga
     2701 tcgcatcaat caacaagccg actgtttctc tggtacttgg cggcagccat tcgatcggtg
     2761 tgccgatcgc tgtctctacc gattattcct ttattgtccc gaccggaacg atggtcatcc
     2821 atccggtccg tatgaatgga acaatcattg gagccccaca gacctatgat tatttcaaac
     2881 agatgcagaa tcgcatttta ggctttattt ccggtcactg cgaggcaaag agagaacgtc
     2941 tcgaagaact gatgctcgat accggcatgc taacaaaaga tctcggaacc attctggtcg
     3001 gagaacaggc tgtaaaagaa ggaatcatca atcagatcgg cggaatccat gaagcgatca
     3061 caaaactgca tgagatgatc cagcagagcc accaggaagc ataagaagtt tccaaaagag
     3121 cagttgctga aattcaggtc aaagtagatg cactttaatt gctctctttg ctgtactgtt
     3181 caaagatctc gcggacgtga ccataaacgc gtccgccttt tgaactctga aatgccttaa
     3241 ccagctgatt gttcagctct gttttattct ggcttccgga gattgcacgg tagactaccg
     3301 aatagctgct cggtgcaagt ttatccgatt taactgccat ccgcaccttt ttccgccatg
     3361 cttccggaag atccggagat gcggaagcct ttgttttcct tccggatgtg gccggtctgg
     3421 tcggtgctgc ggatgtcgat gcgaccgatg aggtctgtgc tgaagcggca tctggctttt
     3481 gcttccggac gaccatctgc ggtggaatat ttgccggaag cgagagtgga gccggaggca
     3541 caatggattt ctggcggatg atatcgatat ttttatcact ccagaagtcg atcacgctgt
     3601 catatccggt atcgttcgtg ataatataaa catgttccgg tttgtccttt ccgatcagga
     3661 aaccaagata agtcgagagc tggaaatcca gataattttt tccggtcttt aaaatcttaa
     3721 tgtattcaaa atgagcagga gactgagaaa gctcaatgtg acgctcaaaa gaaatattct
     3781 tcgtatgctg actgtagaag acaatcaccg tgtcctcggc ggtcagttct tttacaccca
     3841 taaaaccggt gtctgtaaca ttttcataat caatcagata taaattcata tgatcgctgc
     3901 cggtccaatc aaaaatttcg t
//
LOCUS       DAWDWH010000200         3902 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_8786_length_3902_cov_9.984403, whole genome shotgun sequence.
ACCESSION   DAWDWH010000200 DAWDWH010000000
VERSION     DAWDWH010000200.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8786_length_3902_cov_9.984403"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    430..439
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    821..830
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          970 a         1071 c          766 g         1075 t
ORIGIN      
        1 ccaaagagaa acggatggaa atctttctcg ccatactacc ctgcggatag acggttccgt
       61 tatgctctgc cggtgtgcgg aagaaataat aatacttgtc tttgatcttg caccagccgg
      121 tcatcatctg tccataagtg gcacttctgg tatccatata atacgtatgc ccctgatctt
      181 tcagccatcc ggcacgcata tagccgtccg agccaaagaa atacctcttc tcaccaatgg
      241 tgatccagcc ggtcaccagt ttgttcttcc ggtaatatct ccactgctga ccgtatccaa
      301 cccatccggt tttacttttg tttgtccgtc tgtcatcacg atagcctttg atataaacgc
      361 cgtccggccc aagatacttg tcatcgatcc aggtatcttt cacaaacgtt ccgtcggatc
      421 caagatacan nnnnnnnnnc atctttttac cgttgtattt cacccatttt tctttctgca
      481 aaacatatct tttattgaaa taataaatct ttccgtcaat cttctgccat cccgtcaggg
      541 catgcccttt tttattaaag tagtacgtct ttccattgat ggtaaaccac tgacgtttca
      601 ggcggccggt accgtcaccg taagaatagc aaagttttcc ggatgcggtt gtcctgaatc
      661 cggctctttc atatgccttc agcagaatcc ctttttttga aaatacatag gtctttcctg
      721 cgatctgccg cacaccggtt gccatgatcc ctgtctttgg cagaaaataa taagttcctt
      781 ttttgagtgt gagccagcct ttctgctttg taccatcctc nnnnnnnnnn gtaacggtta
      841 ccttcctcaa ccttccactt tccttttgca aaagcggtta cggaaagcaa catactcagc
      901 atcagcactg cgagcacacc aaaccccttt ttcttcattc ctgattcccc ctttctttac
      961 ttcttatgtc ttcaggactc tttcctggca accggtccgg taaacacctt tctcggacag
     1021 agcgatgcac aggttccgca gttggtacat tttgaagtat cgatatgtgc aatattgttc
     1081 tccacagtaa cggcaccgga aggacattct ctctggcatt tcttacatcc gatgcaccct
     1141 gcctcacatg cctgcataac cagctttcct ttatcttttg aagcacagcg taccagataa
     1201 cggctttcat atggcacaag ctcgatcaga tgacgcggac aatgctccac gcatgctcca
     1261 cacgctctgc atttctcctt gtctaccact gctactccat ttataacatg aatcgcatca
     1321 aatgaacaga ccttttcgca ggttccgtct ccgatacatc cataatgaca ggttttcggt
     1381 ccgcctcccg gaataaaatc catcatctca cagtcgtgaa ctccataata atgacaggat
     1441 tcttttgctt tgtcacaggt tccgccacat tttacaaagg caactctgcg gatgctctct
     1501 cctgctttct gccccatgat cctggcaatc tttgctgcca cggcatctcc ccctaccggg
     1561 cagccattta ccggagcctc tccgtttgca atcgcctttg ccatcgcatc acatcctgca
     1621 tatccgcagc cgccgcagtt atttcccgga agctgttctc ttacctgctg ttctttttca
     1681 tcaatttcta ctgcaaattt tttccctgcc aatccaagga aaagtcccag aagcagtccg
     1741 gtacctccta cgatcacagc cgcaagtatg atctccatgc tcatccgcca ctcctccttc
     1801 ctatttcaga ctggaaaatc caaagaatgc gatcgccatc agacaggctg taagaagaac
     1861 gatcggtgat cctttaaaag attccggtac atcattgtat tctgtttttt cacggatacc
     1921 ggccatcagc acgatcgcga tcgtaaatcc gaccgccgtt gccaggccat ttaccacgct
     1981 ctctacgatg ccgtagcttt tctgtacatt gatcagtgcc acaccaagta cggcacagtt
     2041 tgtcgtgatc agcggcagat aaacgccaag tgcattgtac agagatgggc tgctcttttt
     2101 cagaaacatt tccacaaact gtaccagtgc cgcaatgacc agaataaaca cgatcgtctg
     2161 aaggtaagtc acattcagcg gaaggagcag aaatttatag atcagagccg tgacaaagga
     2221 tgagatcgtg ataacaaaga taacggctcc gcccatgcct gctgcggtat ccattttctt
     2281 ggatacacca aggaatgcac acagtcccaa aaactggctc aaaacaacat tgttgataaa
     2341 cgcagtgccg actgcaatca taagtaattc tctcataacg tctcccctcc tttacaggaa
     2401 cttgcacagg aggcacattc tcctgtgcag ccgctgccga tctttgtctc tttacctttt
     2461 tcctgcattt tacgtttcac gcggttctgg gctgctgtca gaagggacag gaccagaaaa
     2521 gctcccggtg caaggataaa gatcgtgatc ggctgataga aagaaggcat cacctgcaca
     2581 ccgaaaatgg ttccggctcc gatcagctca cgcacgatac cgatactggc aagaccgatg
     2641 gtaaatccaa gtcccatacc gacgccgtca aagaaagacg ggatgaccgg attgaaagaa
     2701 gcataagctt ccgctcttcc aaggatgata cagtttacta cgatcagcgg aatgtaaata
     2761 ccaaggctct gatacagaga cggtacatac gcctgaagca aaaactgtac gatcgttacg
     2821 aaagatgcca caaccacgat aaaggccggc atacgcacgc cgttcggaat gactttacga
     2881 agtgctgaga tcatcaagtt tgacatggca aggatcaccg ttgtcgtaag tcccatgcca
     2941 agtccgttca tcgctgaggt ggttactgcc agcgtcggac acataccaag catcaacaca
     3001 aaggtcgggt tttctttgat gataccgtta tataaacgtt ctcctgcttt gctcattgta
     3061 agatacctcc ttccttaagg ctttcataaa aacgaagtcc cgcattgaca ccgtttgtca
     3121 ccgctttgga agtaaaggtt gctccgctga tcgcatcaat ctcgttttcc gcggaagcac
     3181 cgttttttgt aaccacgaac tgatctacga tttttcctgc gaactgtcct ttgaattctt
     3241 cgtcttttgc acgcatgcca agtccggctg tctcacttaa ggaaagcatc tcaacgcctg
     3301 tcaccgttcc gtccatactg acacccatgg agaaattgat gtcaccgccg tatccttcat
     3361 gcgaagtaat gttcataacc acacctgtca ctgctccgtc cttttctgcc agataaaaat
     3421 cttccagagt gtcagctgtg ccaaatccgg cctgatccag gacatcggct gcctctgtga
     3481 gcagttcttc tttgttatcc ggctccgtca gatcctgtgc atccggaaat acagcaagat
     3541 aagcttcttt ctgtgctttt tcctctgcct gtgcgatcgg ctgttttgta atgctgtaga
     3601 cagctccgag cagtaaaccg gaaaccagtg tgatcgccat cagtaccagt gcttctttta
     3661 taatcttatt catgcttttt tcctcctttc ccaaatgctg tcggaggtgt tactttttca
     3721 atcaaaggca ccagaaggtt tgaaaggatg atcgcataag atacgccctc cgatgcactt
     3781 ccgaggaaac ggaaaacccc ggtgaaaata ccgatgatgc atccatacac aatctgaccg
     3841 tttttggtga tcggtgaggt tacataatcc gttgccataa aaaatgtacc aagcatcagt
     3901 cc
//
LOCUS       DAWDWH010000201         3809 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_8998_length_3809_cov_10.426745, whole genome shotgun sequence.
ACCESSION   DAWDWH010000201 DAWDWH010000000
VERSION     DAWDWH010000201.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8998_length_3809_cov_10.426745"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1081 a          791 c          993 g          944 t
ORIGIN      
        1 gtattctgca tcggatttcg aagtgagctg gaagcagtcc gttttgtcag agagtcagga
       61 tttgatgttt taaataaaaa tgcgactcag cttaccgtct cggtttccgg agaactgcag
      121 aattttatca aagtgctgtc agggtataaa gtgctgacca tggagagcat tccgcagacg
      181 ttggaagaga cctttaccta tctgtatgga ggtgacctga gtgattaata ttccaatact
      241 gaagaaaaat ttcagagaac atatctggtt gtggggcatc gtcaccgctg tactggtact
      301 ttatctgttt ctgctgttgg ggacatacgc accagatgga aaagccggtg tgatcctttt
      361 attttccggg ctgccgtccg cagttcagac agcacttggg gcaggcaatt acggactgtc
      421 tttaagtggc attttgggag gctatttctg ggagaatatc tttacactgg taccatttgt
      481 attcagtatc ccggtggcgg catctttcgt tgctggcaaa ctggaaagcg gtgcttttgc
      541 ctggattctg tcggcacctg tggagcgtga aaagcttttg tttactcaga tttattcttt
      601 tacatttgcc ctgtttgcat tgttttttgt aaattgtctg gtcggaatag tgtgcggcct
      661 ggccttttgc ggatcgtatt ttgtgatttc cgagttctta ctgatgtgcc ttggcggatt
      721 ctgcctgcat ttctgccttg ggggcctgct gtttgtgata tctgcaatgt gtgatgagaa
      781 agcactggca gtgatgatca cgacagggat cgtactgtta ttttatattc tgaaaatgct
      841 cgcttcgctt ggggggattt tcggatatct gaaatatgtg acgatcttct ccatgtatct
      901 ggcggacgat gtgatcgacg ggggcatgtt ttttatatgg aaatatccgg tactgatctt
      961 ggcgggcgtc ctgctttact ggctttccat gcgaatattt gagaagcggg atctgccgct
     1021 ttaaaagagg agtaaataat tgaaaaaatt atacatagca gaaaaaccga gtgttgcaca
     1081 ggaatttgcg aaagcgttga agatccgggc ggcaaggaaa gatggctatc tggaggggga
     1141 agaggccgtt gtgacctggt gtgtcggaca tctggtgacg atgagctacc cggaaattta
     1201 cgatgcttct tataaaaaat ggagccttgc gacgcttccg tttctgccgg agaactttct
     1261 ttacgaagtg atcccggcga cgaaaaagca gttcaaagtg gtcagcactc ttttaaatcg
     1321 aaaagatatt gatgtgatct atgtctgtac cgactccgga cgggaaggag aatatattta
     1381 ccgcctggtg gagcaggaag cacatgttca gggcaagaaa aggctgcgtg tgtggatcga
     1441 ttctcagaca gaagaagaaa tcctacacgg gattgcgaca gcaaaagatc tttccgcata
     1501 cgataatctt gcaagtgccg cttatctgcg tgcaaaagag gattatctga tgggcatcaa
     1561 tttttccaga cttttaagtc tgaaatacgg gcagtccatt tcaagctgca tgggaacaag
     1621 atacacggtg atatcagtcg gacgtgtgat gacctgtgtg cttggcatga tcgtcagaag
     1681 ggaacgtgaa atccgtcagt ttgtgaaaat gccgttctac cgtgtactgt cttccatggt
     1741 atgcggtgaa agcacgatcg aaggcgagtt ccgtgccgtc aaaggttccg cgtatttcca
     1801 gtctccgaag ctctacaaag agaatggatt ttcgcataaa gaagatgcaa aagagctgat
     1861 agagaagctg agcgatccca aaccggtgca ggctgtgatc gaaaagctgg aacgcaaaaa
     1921 agaaaacaaa aatccgccgc tgctttataa cctggcggaa cttcagaatg actgctcgaa
     1981 gcgttacaaa atcagcccgg acgagacctt aaagattgtc caggaactct acgagaaaaa
     2041 gctggtcact tatccgagaa cggacgcccg agtgctgtca acggcagtag caaaagagat
     2101 ccacaaaaat atccgcggac tttccgggtt tgcaaacgtg cagtcttatg cggcggaagt
     2161 tttagaaagc acggcctgga aaaagatcgc atcgacacgt tacgtcaatg acaaacagat
     2221 caccgaccat tatgcaatca tcccgaccgg gcagggactt ttggcactgc ggagcgtaag
     2281 tgccctttcg gtaaaggttt atgagctgat tgcaagaagg ttcctcagta ttttttatcc
     2341 gccggccatt taccagaaaa cgacactggt ttcaaaagta cagcaggagt cctttttctc
     2401 aagtttccgt ctgcttctgt ccgaaggata ccttaaagtg gcaggcatgc cggaagaaaa
     2461 taagaaaaaa gacgatacag aagatgtgaa atgtgacgaa aatctgcttc aggcactgca
     2521 gaaatgcaaa aaaggtgata tacttccggt gaagggcttt tccatcaaag aaggcgaaac
     2581 ttcaccgccg aaacgctata catccggttc gatgattctg gcaatggaga atgccggtca
     2641 gctgatcgag gatgaagaac tgcgtgccca gatcaaagga agcggcatcg gaacgagtgc
     2701 aaccagggca gagattctga agaagcttca ggcaaacaaa tacattgccc tgaataaaaa
     2761 aacgcaggtt ctgacaccga cacatctggg ggagatgatc tttgatgtgg tgaacaattc
     2821 aatccgttcg ctgttaaacc cggagcttac ggcaagctgg gaaaaagggc tcacctatgt
     2881 ggcggagggt acgatcactt ccgaagaata tatggaaaag ttagagggct ttataaaaag
     2941 aagaaccgat gccgtacttg gcagcaacag ttcctatgct ttgcggaaac tgtttgacga
     3001 atcggcagtt ttctataaaa aacagcaaaa gccgcagagg cgattaacag gaggtaagaa
     3061 agatggaaac atgtaaaatc aaaaatctct ataatctcga ggaaacgatt gcaaaagatc
     3121 tgtttgaagg agccgtttat ccgtgggaag tgcttccgaa gatcagcgat tttatcaaaa
     3181 aactgggagc gaccttaagc gaagaagaat acgaaaaacg cggggaagac atctggatcg
     3241 caaaatccgc aaccgtagca ccgaccgctt acattcacgg accggcaatc atcggaaaag
     3301 aagcagaagt acgccactgt gcattcatcc gcggcaatgc gatcgtaggt gaaggagccg
     3361 ttgtcggcaa ctccaccgag ctgaaaaatg tggtcctgtt caacaaagta caggttccgc
     3421 attacaatta cgtcggtgat tccattcttg gatacaaggc acatatggga gccggttcga
     3481 tcacatccaa tgtcaaatcc gataaaaaac tggtagtggt aaaagatgac aaaggcaatc
     3541 gcatcgagac cggactgaaa aaattcggtg ccatgatcgg cgataacgta gaggtaggat
     3601 gcggctccat cttaaatccg ggaaccgtag tcggaccgga cagcaatatc tatccgcttt
     3661 cctctgtgcg tgaagtgatc ccggcacaca gcatctacaa aaaacgcggc gaagtcgcag
     3721 aaaaagttgc tgactgagaa ggagtctgtt ccagaaagtc cccaactatg atgcctacgg
     3781 attgttccgt gcgttgcact cccacaatc
//
LOCUS       DAWDWH010000202         3804 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_9013_length_3804_cov_9.661243, whole genome shotgun sequence.
ACCESSION   DAWDWH010000202 DAWDWH010000000
VERSION     DAWDWH010000202.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9013_length_3804_cov_9.661243"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1216 a          743 c         1008 g          837 t
ORIGIN      
        1 tattttggat ctcggggtat cctcctacca gttggatacg gctgagcggg gatttacata
       61 ccgcgaagac gcaccgcttg acatgaggat ggatcagcgt caggatatga ccgcaaaaga
      121 tattgtgaat ggatacagcg agatggagct gtaccgtatc atccgcgatt acggcgaaga
      181 gaagttcgca aaaaacatcg cgaagcacat tgtaaatgca aggcagcaga aagaaattga
      241 aacaactggg gagttaattc aaatcataaa agcagccata cctatgaaag tccgagcagt
      301 aggcggacac ccggccaaaa agacctttca ggcaattcgg atcgaactga acagagaact
      361 ggaagtttta agggattcgc tggacagcat gatcgatttt ttgaatgacg gaggaaggat
      421 ctgtgtgatt acattccatt cgctggaaga tcgaatcgta aaaaacaact tcagaaaaaa
      481 tgaaaatccg tgtatatgtc caagggagtt tcctgtctgc gtatgcggca ggaaatccaa
      541 gggctttgtg gtcaccagaa agccgattat accgggtgag gaagaactaa catataataa
      601 acgagcaaaa agctccaaac ttcgtgtttt cgaaagaaga cgggaggaag aggagtcatg
      661 aaacaggtaa acagagacaa atgtaagaga caggagggtc gatatggcag agagaaatta
      721 tcgcagagat gataacataa gacagacagg tcgatatgct tatattgatg gcagtaccgt
      781 tcgcaaatta cagactcgcc ctgtaccgga aaagagagaa accgagagaa gacaggaaca
      841 gccgcgacgc agaaaacgga cgagtgccaa aacaagacgc aaccgtgcac gtgcactgca
      901 gatgcatctg cgtgacgtta tctttcttgg aattgcagga atgatcgtgg ttgtttcatg
      961 catcaacttt cttcagaaac aggcattgaa tacatcctat cgtaatcagg tggcgacaca
     1021 ggaggccaag cttgcagatc tcaaagaacg caatgatgct gcctatgaaa gtgcactgaa
     1081 gtccgtagat ctggagacga tccgcaatat tgccatcaac aaactgggta tggtttatgc
     1141 cggagaagaa caggtaaaaa cttacgatga caagagcagc gactatgtaa gacagtacag
     1201 tgatgttccg acagagtgag taatcagaca ggtggtataa agtggcagaa aagaaaagaa
     1261 aaaaaagaaa gcacttttca aaactaatgc agaaaaaact ggtgggaagt tttgcattag
     1321 ttttgtttgt gttactggga ttaaatttaa gaattgtata tattacagcc aaaaacggcg
     1381 ataattatgc aaaacaggtg ctgtcacaga aacagtatga cagcagaacg atcccataca
     1441 aacgcggaga gatccaggat cgcaatggaa atgtgtttgc aaaaagtgaa aaggtctata
     1501 atgtcattct cgactgtgta gcggtaaacg gtggtgatga agaagaacag gcaaagctga
     1561 aagagcagga aattgattac attgaaccga cgatcgatgc acttgtgaaa acattaggtc
     1621 tggatgaagc agagcttcgt tcactgatta ctgatccggc aacatccggt agtcgttatc
     1681 aggttctgaa aaagggcatt tctctggaag aaaagcagac atttgaggac tatacggaga
     1741 tcgattctga aaaggagtac accaagacag accttgcaag gcggcggtgt gttcagggtg
     1801 tctggtttga agaagattac gtccgggatt atccgctgaa aacacttgcg agcaatgtga
     1861 tcggagtcgg aagcaattcc ggcggtgcaa gcgggttgga atcttactac acagatgtct
     1921 tgagcgggac agacggcaga gagttcggtt atctggatga gaactccgac ctgcagagga
     1981 cgatcgttga accgaccaat ggaaatacaa tcgtttccac cctggatatc aacatccagc
     2041 aggttgtgga aaagtacatc gctgaatttg atgcagagta cggcaaagat gccgaggacg
     2101 gcaaaggtgc gaaaaacatc ggtgtcatcg tcggaaatcc gcagaacggc gagatttatg
     2161 cgatggcgag caaccacgga tttgacctca cggatccgga cgatttaagc gacaagtata
     2221 cggacgcaga gctgaaatcc atgacagagg aagaaaaatc agatgctctg aatgagaaat
     2281 ggtacaatta ctgcgtatcc gaaagttttg agcctggctc tacgtttaaa ccgatcgtgg
     2341 tagcatcggc actggagatg ggtgcgatca cgacagatgc tacctacgtc tgtgacggtg
     2401 gagaatttgt caccgataca gaaatcaaat gtgataatat ttacggtcat ggagatgaga
     2461 cgttaagtga tgttatcaaa aattcctgta acgatgcgat gatgcagatc ggaatgaaaa
     2521 tgcagatcac accattcctg aaataccaga gactgtttaa tttcggaagc cggacaggga
     2581 tcgaccttcc gaacgaaagt accggtgtac tgtatgacag ggacagcatg cacgaggtag
     2641 agctggcgac caataccttc ggtcagactt ttaccagcac gatgatccag gaatatgcgg
     2701 catttaatgc agtggtaaac ggcggatatt attatgagcc gcatatggta aagcagatcc
     2761 tggatgataa cagcggagtg gtaaagaata tcaatccggt ccttctgcgt cagccggtat
     2821 ccacgaaaaa tgcagatttt gtgttaagtg ccatggagca gactgttctg gatggtaccg
     2881 gaaaaaaagc aagagtgccg ggctaccgtg tggcaggtaa aacaggtact gcagagaaga
     2941 tcaatccgga gaccggaaca cgttgggaag gaaaatatct ggtatctttc atcggatgtg
     3001 caccggtgga tgatccgcag cttacagtgt atgttgttgt cgatgagcca aacgttgaaa
     3061 atcaggaaac tggtggttac tctatgatca tttccagaaa gatcatgatg gaagtgcttc
     3121 cgtatctgaa cattccacag acggaagaga tcacagatga gttgcttcag gagctggaac
     3181 tgaccagaga agatgtggaa aacggaagaa acgcagctct gatcaaagaa aaatcagaga
     3241 caacggagac agatgaatac ggaaatgtca tctcttccgg aacgaccgct gagacggacg
     3301 agtacggaaa tgtcatctct tccggtacga ccgctgaaac ggacgagtac ggaaatgtca
     3361 tttcctctgg aacgacatcc ggcagtacat cctctgcgac aggcacaacc gatgctcaga
     3421 catcacagac ggatgcttat ggaaatgtga tcagtggcag cgattccacg acgcagggta
     3481 cgacggacga tgagggatcg gcgtttcatt caaatatacc ggctccgctc cagtcacagg
     3541 acagtggtga cggcgtgagt acgcaggact ggatcaccaa cgaaaagctt ggtattgatg
     3601 aaaataataa cagttattag tcaaatggat attcgcaatt gaagcagggg acttccttga
     3661 tttggttaag atataaagat gtgggtgtca aaagtcctca actatgatgc ctacggattg
     3721 ttccgtgcgt tgcactccca caatcctctc cagtattcgc atatcgtggg attatcatcc
     3781 cacttttatg ctcatatcgg ggcg
//
LOCUS       DAWDWH010000203         3804 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_9014_length_3804_cov_9.381168, whole genome shotgun sequence.
ACCESSION   DAWDWH010000203 DAWDWH010000000
VERSION     DAWDWH010000203.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9014_length_3804_cov_9.381168"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1664..1673
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2440..2449
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2722..2757
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3316..3325
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          870 a         1080 c          758 g         1030 t
ORIGIN      
        1 acaacacaga ctcctgtgtc tgaatttggt tcaaattctt ctgcttcttc ttctgttccg
       61 atcaccgtca ctttagaaac acaccatcct cggatgcctt ctacctccgg atgattggca
      121 tttccgatga tcacgatctc tcttccgttt tcgccttccc gcctgacaat gcgatgtatt
      181 ttctttacaa atggacaggt cgcatcggca agattcagtc cgttttcgca gatcagatca
      241 taaacctctt ccgcaacgcc atgggaacgg atcacaacgg tacccttttt taccgaaagc
      301 agatcttctt ttccattcag cacctgtacg cctttttctt ccagatcccg cactacctct
      361 tcgttgtgga tgatcggccc gtaagtgtaa accggctctt tttcttttgc tgccagatca
      421 tagacctggt caacggcacg ttttacgcca aaacaaaaac ccgcagtctg tgcttttatc
      481 actttcattg cttcacttcc ctgtaacatt ctttaatacg ctcaaccacc tcatcgatcg
      541 acagatccga agaatctacc agaatggcat cctctgcctg gcgaagcggt gcgatctctc
      601 ttgacatgtc acgctgatca cggtcccgga tctcctcttc gatctcttcc agtgtgtgcg
      661 tatctccctt ttcctgctgt tccaggaaac gtctctttgc acgcgtttcc acgcttgcag
      721 tcagataaat tttcagctgt gcatgcggca ggatattggt gccgatgtct cttccgtcca
      781 tcaggacatc ttcttttgcc gccagatctc tttgcaggtc aagcagtgtg gctcgcacgg
      841 ccgggtttgc ggaagtcacg gatgccatct tgctcacttc ttctgtgcgg atcagtccgg
      901 tcacgttctc cccgtttaag atcacctgct gttcgccgtc atcgctgtag gcgatcgata
      961 tggaaatctt gctgcaggct ttctgcactg ccgcatcgtc gtggatatcg atctgattct
     1021 gcatcatata aactgcgatc gcacgataca ttgccccggt atcgacataa ataaatccaa
     1081 gctcttttga cacacgtttt gcgatcgtgc tttttccggc tcctgccggt ccgtcaattg
     1141 caatattaaa actcatattt tcctcctgca tggtatctat gcttcgctca tcgccagtgc
     1201 tgccgcatag gcagtggacc aggcgatctg cagattgaat cctccggtca cagcatccag
     1261 atccagtacc tcacctgcaa aatacagtcc ctgcaccagt ttggactcca tcgtggacgg
     1321 attgacttct tttacactga caccaccttt tgtgataata gcttcattat aatcgcgaag
     1381 ccctgttacg gtcaatggca aatttttgat cagctccaca aatccgaggc gttcttctct
     1441 tgatacctca tttacctttt tctccggatt gatgccggac aactcgatca tgaccggagt
     1501 cagttttgcc gggaaaagtc cgcccagtgc gtttttaaac tgtctgtttt ttgcttcctc
     1561 aaaatcacgc aggattcggt gatctagttg ttcttttgac aatgccggct tcaggtcgat
     1621 aaacatagaa agttcttttt ttcgtgccgt tttcgcagtg taannnnnnn nnnaaataaa
     1681 atctccagtt tttcgtgtat gcgatctcgg atccggtaac agcacagtat cagacgtatc
     1741 agctcatgat acagaagaat gaccatgcac acaagcggga tcgccagtac catctgccag
     1801 cagaagatgc tgtggattcc aagaaatccc gggaacagca gcacacccac cgaaaaaact
     1861 gcgatcacgc agacacagag aatgcctctc agccagttca acggcctgca cacacgaaat
     1921 acgaccatga tcccgactgc tcctccgacc aggagatcaa aggtataggt aaacacttct
     1981 gtttctttcc agaacggtga caaaacctga accacaagca gggtgacaac catagtaatc
     2041 gccgccggaa gtgccgtctg catcacatga cgcaaaaatc cagtacttgt cacctcttcg
     2101 gtatgctcca gtgtcagcac aaaactcggg aaaccgatgc aggctgcact gatcagagac
     2161 agctgcaccg ggataaacgg atacgcccgt ccgagaagaa taaaaatgac acacagcagg
     2221 atggaataca gtgtcttggt cagatacagt gcgctgaccc gctcgatgtt ggcaatgatc
     2281 gtccttcctt cgcttacaat attccgaagg caggcaaaat ccgaatccag caggacaata
     2341 tgtgccgact gttttgccgc atcacttccg gctgccatcg caataccgca gtctgcatcc
     2401 ttcagtgcaa gcacgtcgtt gacaccatca ccaaccatan nnnnnnnnng accactttct
     2461 tttccgactg ataagcatgg acaagcttct gcttctgttc cggcttcacg cgtccaaaga
     2521 cattatattt ctgcacggct tcccggtaac tttgcggatc atctccgagt gtggaggcat
     2581 ccacgtattt ttctgccccc agaagtcctg cttttccggc aatcttcgat accgtcaccg
     2641 gattgtcacc ggagatcacc ttgatctcta ccttctgccc acggaagtac tgaaagacgt
     2701 ccggtgcttc tttccggatg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnacg
     2761 atcagtccca tcggtgacac ctcactgaca cttccatctt ccgcctgaac agaaccgcat
     2821 tttccaagca acaggacacg atagccctcc tcggaatact gattgacttt tcgaaggatt
     2881 tccgcccctt cttcgtcatc ctgcaataaa aactccggtg caccaaggac ataacagccc
     2941 ttttcttcga actcgatcgc acggtacttt cgttcggaag aaaacggaat tttctgaacg
     3001 accgtaaaac ggtccatccg gtgaaaataa cgttttaatg cttcctgcgt gttgttgctg
     3061 tcttcaaaag caaacgtaag ctcattcatg atctcgctta cttcttcttt tttcacctct
     3121 ccaaggccaa gcacctctgt aacttccagt tccccggtcg tgatcgttcc ggttttgtca
     3181 aggcagagca cgttgacgcg tgccagtgct tcgattgctt ccatctcctg cacgagtgcc
     3241 cgcttttttg caagtcttcc gacgcccagg ataaacgaga tgctggtcag cagcaccagc
     3301 ccctccggaa tcatannnnn nnnnnccggc aacggttgcc acgatagctt cgttcctggt
     3361 catcccctga gcactgatct gagaacggaa cagcagaatg ccgatcggaa tgatcagaat
     3421 actgatcacc gctatgatcc gcttgatcgt attctgcatt tccgaagtgg cacgtttttt
     3481 cgtcttggct ttccttgtca gctctgcggc ataattctca tcaccgacat gaaccacacg
     3541 gccgacaccg ccgcccgcaa ctacaaaact gccggacatc agctcatctc ctgctttttt
     3601 ctttacaggt tttgattctc cggtcagcat ggattcatta acctccaaac cgtcacattc
     3661 aagaaccgta cagtctgcac agatctgatc gccgtttatc acaatcagaa tgtcatcttt
     3721 caccacttct tctttatcta tctcatattg ttcaccttct cttaccacat gagcttttga
     3781 agccgtcaga accgtcagct tgtc
//
LOCUS       DAWDWH010000204         3780 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_9058_length_3780_cov_29.539060, whole genome shotgun sequence.
ACCESSION   DAWDWH010000204 DAWDWH010000000
VERSION     DAWDWH010000204.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9058_length_3780_cov_29.539060"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          962 a          933 c          546 g         1339 t
ORIGIN      
        1 tccagataag ttcccatctc aatatagttt ctggcaaaac gtgaaaaatc ttttaccaca
       61 atacactgca ctttattctc cctagcaagt tccagaacct gcttaattgc cggacgctcc
      121 atactggaac cagaatatcc gtcgtcgtag aactcctgaa atgacataga agccagttcc
      181 gggatattgg aaatataatc ttttaccagt tttctctgat taataatgct gttactttct
      241 ccctctgagt catcttccat ggaaaggcgg taatatccaa taatcagttt ctgatcactc
      301 atgttctacc gcccccttaa acccgaagtt gatttccagt tttccatcac catatagata
      361 catactttca atcaagcctt ctgcaagttc cgcattgatt ctggttgtcc catccagttc
      421 cagcaggctt cgtaaaaatc ttgcttcttc tttctgctgt ttttccagct tttgtatggt
      481 atgctccaga gttctctttc tctcttcgca gaatgctttc cagttattac ggtcatcttt
      541 catttctata taggcttctt tggaaagttc accctcttta tattgcatga atgcctgtgc
      601 cagtttttct gaacgttttt ccatatctgc atccagtttc ctaatctctg tttgaatttc
      661 tttgattttg gaaagaaata ccgcattgct tatagcagac atatcctttt tccgtaagcc
      721 agataactga aactgtctgg tcagctccga gcggacaatt ttctgcaatt tttcttcaga
      781 gatagattta tgactacatt tcctttcatc ccgatactga gcagcgttgc aaaaataata
      841 cacattgcct ctgtatctgc gtgtacacat tttccgcttg cagtctccac aatagaatac
      901 attataaaat gctctttcat cctcttccca tcctgccgta gtttttgttg ttttctgttg
      961 tgctgctttt aacctgactt gtgctttgtc aaacagttct cggctgataa tcggctcatg
     1021 ggcatttggc gtaataatcc actggctttc atccagtata tcacaccatt tttcacctct
     1081 ttgaaatctg gattcatatt ttctctgaac cagatcacca taataattat ttcgatttaa
     1141 aaccgcacgt atcgaagaat ttccccactg atgaagattc tctccatctt gacagtacac
     1201 atgatgatat tggttatagt ccgaaatccg atgtacccca tcttcaaaca gcctgtcaat
     1261 aatgctctgt atcccatctc ctgaagcata ctcttcaaaa atcctgcgga caatctttgc
     1321 agcttctggt tccacaatca acttataaat tccatttacc ttttccacac aatatccgta
     1381 tggagctgta gatcccacat actcaccgtt cttctgtgca atacgttttg ctgcccgttc
     1441 ctttgcagaa atgtcttttg cataagcatc attcaccaaa ttcttgatat tcatggataa
     1501 ttcctgattc ttagcacccg gtgcaaatga atcatagttg tcacaaacag aaacaaaccg
     1561 cactttcata aaagggagaa tcttttccaa ataatttccg gtttcgatat aatttcttcc
     1621 aaatcgtgag aaatccttta ccagaataca atttatttta cctgccctga catcattcat
     1681 catccgttca aatcccggtc tgtcaaaatt tgttccggtt tttcccaaat cagaataaat
     1741 atcatataca gcaatctcat actctctgtt cggattttca ttgtgcttct gaatgaactc
     1801 ttttatcagc gtaacctgtg tttcaataga ttctgacttt ttttcatcac tgtctacgga
     1861 taatctggca taaattgcag ccatacatac cgaaattcca agaactttct tctctgcctt
     1921 cttcttatat ctttttgctg tccttgccat ttatctcacc tctttcctgc actctgtccg
     1981 gtgttccgca taaaaccgtc ttatgacttt cattttctca atcgtatcct gataacggat
     2041 atgaattttg atctgtttgt tttcataaat atagatttta tctacggtca gtgccagcaa
     2101 tgtgcgatcc agttctttga tttccagtga tttcttccag tcctccaact gaacagttgc
     2161 agacactcca ccctcaaaca tttgcttcac cagctttttc tgattttcaa tcatctgctc
     2221 cagttcttca cattttcttc cgtaactttc ccgaaaatca tcgaactcct ctttactgat
     2281 cagtccctct ttcaagtcat cacttaaaga cgctttcagg ctgtaataac ggttatattc
     2341 ttcctgcaac ttactaatct gcgtatcata accgattacc tgatcgtaac tgactttcat
     2401 ttcacaaagt tcttccataa tcatctgata gtctacgaaa agtgccgtat atgcctgaat
     2461 ctctttcaat acaatccttt tcagcacctc ttccggaata ctgtgtctgg tgcaatctcc
     2521 acctttattt tttgtctgac aaatataaaa ggcttttttc tttcccttat actgatttac
     2581 cctgcgtatc atcggtgtat ggcaatctcc gcaaaataca aaacctgaaa aaaagtttgc
     2641 actgtctgat gttttcgatg ccctgccatc atattgaagc agcttctgaa ccacatcaaa
     2701 atcattctgc ctgataattg ccggatgcgt attttctact ttcacccact ctgattctgg
     2761 cttatccagg cgttgcttta ctttatagct gattcgttcc tgcttgccct gtaccatgtt
     2821 tccaatatag acttcattgg tcagaatcct tttgatctgc actgccgacc atttcggtgt
     2881 atctgaacta tggaatccgg aattgtaatt ttcaccattt gcctttttat attcttttgg
     2941 cgactgcaca tgacgtacat tcagtttttc tgcgattgct ccaagactga atccatcaat
     3001 tttccatgaa aatatttttc ttacaatatc cgctgcataa gaatcaatca ccagacaatt
     3061 cttattctcc ggatctttgc agtaaccata cggggcaaat gctccaataa attcaccttt
     3121 ttcacgtttg attttctggt ggcttcgcac tttaccggaa atgtcccggc aatagctttc
     3181 atttacaaaa tttttgatcg gaactacaaa tgacttctct gaaaaatctg ctgttttact
     3241 gtcgaactgg tctgtaactg aaataaaacg cacatttaaa gccgggtagg tcttttcgat
     3301 ccatcgcccg gcttctatat actctcttcc gaatctggat aagtctttta caatcacaca
     3361 gtttactttt ccagcttcta tatcagttgt cattcgttta aactcaggtc ggtcaaaatt
     3421 tcctcctgag tatccgtcat ccacatatat atcaaagatc tgaatatccg gctggctttt
     3481 tacaaagctc cgaagtaact ctctctgatt tgcaatgctg ttgctctctg acttcgcacc
     3541 gccctcttcc atatcatctt tcgataaccg aagatacaat gcagcatcgt acatatctgg
     3601 catattcatt tgcatttgtt ccattttaca tcgctcccaa cttacttatt ccattgaatt
     3661 tgaagtcaga aaccatgtat cacgttcatt ttccctgact tcacattaac ataacatctg
     3721 tagcttcgca agatattttt tcaagacttg catctaatac atcaactccg ttctttttct
//
LOCUS       DAWDWH010000205         3770 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_9081_length_3770_cov_12.225034, whole genome shotgun sequence.
ACCESSION   DAWDWH010000205 DAWDWH010000000
VERSION     DAWDWH010000205.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9081_length_3770_cov_12.225034"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    432..441
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2617..2626
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1223 a          767 c          974 g          786 t
ORIGIN      
        1 aatgccatgc ctatcctgga gaggaacaac attccgtata caccgtatac gtctgagtgt
       61 gatgaatttg tggatgccct gaaagtggca gacacactcg gacagccata tgagctgctt
      121 tataagacac tggtcacgat tggaaacagc agaaattatt ttgtgtttgt gataccgatc
      181 gccgaggagc tggatatgaa aaaggcagcc aaatcagtcg gcgaaaaatc cgtttccatg
      241 atccatgtca aagatatcaa tcaggtcacc ggttatatcc gcggaggctg tacggcaatc
      301 ggcatgaaaa agcagtatgt gacgagaatt gatgaaagtg cacagaaact ggagaagatg
      361 atcgtcagcg gaggccgcct cggcgtacag atcgagctga aaccggaaga tctgaaaaag
      421 gcaagcaggg gnnnnnnnnn ncaggggaga gtttgccgat attattttca aacagccgga
      481 ataagtacgg acaatcagca gggatttcat tcccatcgga taccgcttcc ggcggattgc
      541 agagttaaac aacatctgag gtaagaggca gatgaaagag aaggagaaga aaatggaaaa
      601 gaaaaaaaag aaatggcttt cgctgtttct tgccgtgatc ctggcattta ccggtctgcc
      661 ggtatcactg atggcagcag gaaatgcaaa aagccagaca caggaaacaa cgaagatcct
      721 gccgagccag acatcgggag gggtcaactg tttttcctac gaatcattca gcggaaagag
      781 ctggacgtac aatgatgacg aggcatatat agacctcgga tcatcgaatg agaaggcaga
      841 agaatgcttt tacagggtaa cgtttaaggg aaatgccatc gaagtttttg caaacaagag
      901 tcacaaccac ggaaaagtga aataccgtgt ggatgacgga gcagagacac tggtggatct
      961 ttatgagagc agccgtacca cgccgcagtc tgtatataaa gcagaaaacc ttacggaagg
     1021 tgaacatacg ctctatgcag ttacgcagaa ggaacgcagt ggcagtgcgg tagtcaatca
     1081 ggtggcttat gtacaggtca cacacagccc gtacattgca aaagatttta aactggaaga
     1141 tcaggggatt tccttaagtg tcggacagag ttatgcaatc tcttacagct atacaccgtc
     1201 ttatgcgaca ctgaacgata tgtcctatac aacttctgat gaaaatattg cttctgtcag
     1261 tgcacaggga acggttactg caaagaaaag cggaacggca gtgatcacgg catccagcca
     1321 gaaggcaggg atttccagaa caatggaagt ggaagtgcga gagcagggaa atacgcttgg
     1381 cggcaccgtc accgatcata acacgcagta cacccagaaa cgttttgcgg aagtttcggt
     1441 aaagaaaaac cgcagcgaga cgctgactgc ctggaagaat gaccgtgcag tcagtgaact
     1501 ggtcttaagt gcgatcggtg gagatttcac aaatgtagcg atccaggcat ccgacctgac
     1561 ggacggcaaa aagaagatcg caaaagaaaa tgtgacagcg acattcatcc gttccacaaa
     1621 agcttatgtt tatggatata tatatggaaa tgatgttccg gaggcaacag aagagaaccg
     1681 tgcggaagcg tcggatattc tctggcagag tacgccgatc gacataaaag cagatacact
     1741 ccagccggtc ttggttgagt ttgcgattcc aaagacagca aaatccggca cttacaaaac
     1801 acagctgacc gtaacagcag atcggctcga taagccgctg gtgtttgagt atgaagtgcg
     1861 tgtgcagaat gcagaacttc cggataatta cagagatacc tttgatattg aactgtggca
     1921 gtatccgtat acgagtgcag aatattatag cgtagagccg ttttccgatg agcatctgga
     1981 gatcatgaag tcctccatgg agctctataa aaaagcaggc ggacatgcga tcactgcttc
     2041 gatcatcgaa gatgcctggg acggacagac atacagtgca aatgatgtac attatccatc
     2101 tatgatcaaa tggatcaaga acggagatag ctttacatat gattataccg attttgataa
     2161 gtgggtaagc ttcaacaaat cccttggaat cggggataag atcgtgcttt acagcgtggc
     2221 accgtggcac aattctttca cttattggga gaacggcaag ctggtaaaag aaggattttc
     2281 tgtgggaagc acacgttata ccacactctg gacggacttc cttaccgatc ttaccgcaca
     2341 cctgactgaa aaaggctggt ttgatgacag ttacatcggc attgatgagc gtggattttc
     2401 aggcaccgct tttgacctga tcgagtctgt gaaaaacaag gatgggaaat gtttaaaaac
     2461 agcaggtgcg atggacagct ttgtggaaaa gaaagacctc gccatgcgag tgacagacct
     2521 gaatgtcgga gatacggcgg cagccgcaca tccggcagat tttgaacagc tggtaaaaga
     2581 ccgagaagca aaaggacttc gcaccacttt atattcnnnn nnnnnnttta tattcctgta
     2641 ccggtcacag accgggcaac ttctccttaa gtgcaccggt ggaaagttac tggtcgatcg
     2701 taaatgcagg caaatccggc acggcaggat ttcttcgctg ggcgtatgat gcctgggtag
     2761 aagatccgct caatgatacc acacacagca aatttgaacc gggagattgt ttcctgatct
     2821 atccggacga aaaaacagca cagaatccga tcagcagaag ctcggtaaga ctggagcgta
     2881 tggcagaggg cgtgcgtgat gtcaacaagc ttgctgtgat caaaaaagaa gcaccacagc
     2941 tggcagatca gattcaaaaa ctttatgcag gtattttaac aactgcacag ggcggacatc
     3001 cggcctttct gaccgatgca cagattgcga cgctgtctgc tgagatggac actttccagg
     3061 cgggcattgc cagtctgaca gatcagtata tcaagctgac gggaagaacg gaagagagcg
     3121 aaacggagat ggagagcgaa acaaagaagg agaccgaaag cgaaacaaaa ccgacggaat
     3181 cggaaacagc cgcaacagaa aaaccagatc cgaagccgca gccggataca aaggagacaa
     3241 agccgtcaga gagtgagaca aaacagcaaa tgccgtccac agagataccg gcaaaagcaa
     3301 aaccggcgaa ggtaaaaggt ctttccgtca aggcagtttc gaaagggaaa ctggttctga
     3361 aatggaaaaa agtcagaaac gcagatggct atgtagttta tcgtgccgat aagaaaaaag
     3421 gcaagtataa gaaagtcaaa gtcttaaacg gagccagaaa gataagcttc accaataaaa
     3481 aactgaaaaa gaagaagacg tactactata aagtatgtgc ttaccgcaaa gtgggcaaaa
     3541 agaaagttta tggcagttat tctgcggtga agagcagaaa agtaaaataa acgaatacaa
     3601 aaaaaacagg aattatgttt agtgaatcag gatatgctcc cttctgggca gacaggtgaa
     3661 ataataaaaa acttgtcact caggagggag catattttat ctcagtcgag aagattccca
     3721 cttctgcaag tggtgagtaa taacaagtct ttctcagtgg gagtacaatc
//
LOCUS       DAWDWH010000206         3766 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_9097_length_3766_cov_12.926435, whole genome shotgun sequence.
ACCESSION   DAWDWH010000206 DAWDWH010000000
VERSION     DAWDWH010000206.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9097_length_3766_cov_12.926435"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1124 a          751 c         1035 g          856 t
ORIGIN      
        1 tgcagcggca agacagatga tccgccagaa aagcggaaaa atcgtaaaca tcgcatccgt
       61 atccggtgtg cttggaaatg ccggtcaggc aaattatgca gctgcaaaag cagggatcat
      121 cggccttacc aaaaccatag cccgcgaatt tgcaggcaga aatattcagg taaatgccgt
      181 tgcaccagga tttattgaga cagatatgac agaaaaactt tctgatacta taagagaaca
      241 ggcaacacag cagattccga tgaaaaaatt cgggcatcca gaagatgtag cggaagcagt
      301 ggcatttctt acatctgatg cagcatccta tatcacaggt caggtgctct gtgtggatgg
      361 cggcatggcg atgtaatgta aaaacaggag gcaagatatg agacgagttg tagtgaccgg
      421 tatgggagcc gtgacaccaa ttggcatcgg tgttgagaat ttctggaaca gtgtcaaaga
      481 aaaaagaacc ggatttgata agatcaccaa atttgacacc acagggttca aggcaagcct
      541 ggcggcagag atcaaagatt ttgatccgaa agaatttatg gatttcaagg cagcaagacg
      601 tatggagttg ttctgtcagt atgcagtggc ggctgcagcg gaagcaatga agaatgcggg
      661 cattgatatg gaaaaagaag atccttaccg tgtgggctgt tatgtcggtt ccggtatcgg
      721 aagtctgcag gcaatggaac gggagcataa aagactgctg gagcgtggag cctccaggat
      781 caatccactt cttgttccgc taatgatctc taacatggca tgcggaaatg tttccattca
      841 gctgggactg aaaggcaaaa gcctgaatgt ggtgactgcc tgtgcgaccg gaacccattc
      901 catcggtgaa gcgttccgca gtattcagat cggagatgcc gatgtgatcg tggcaggcgg
      961 tacagaagca gccatctgtc cgatgggtgt cggaggcttt tccgctctga cggcactgtc
     1021 ttctgcgaca gatccgaaac gttgttccat cccgtttgac aaggatcgca gcggatttgt
     1081 gatgggggaa ggtgcaggtg ttgttatttt agaagaactt tctcatgcac aggcaagaaa
     1141 cgccaatatt ctggcggaag tggtcggcta tggctgtacc agcgatgctt accatatcac
     1201 atcaccggcg gaagacggtg agggtgcggc aagagcgatg agtgatgccg ttgcagaggc
     1261 aaacattgat aaaacagagc tgacctatat caatgcacac ggaaccagca cacatcacaa
     1321 cgacctcttt gagaccagag cgatcaaaaa agcctttgga gagcatgcgt acaacatccg
     1381 catcaattcc acgaaatcca tggtcggtca tatgctcggt gcagccggtg cggtagaatt
     1441 tatcacctgc gtcaaagaac tggaagaagg ttatatccat ccgaccgtcg gatttctgga
     1501 agcagaagaa gagatggatt taaattatgt gccagataca gaaatcaggg aatcgtttga
     1561 ttatgcctta agcaattcct tcggatttgg cggacacaat gcaagtattc tgatcaaaaa
     1621 gtatcgctag gaggaatcag atatggagat cgaaaaaatt cttacgctga tcgataaagt
     1681 ttctgcatcc gggctgactg aatttacttt agaagaaggc aatcttcagc tttgcatgaa
     1741 aaaagaaaaa gtcgtaaaga tctgcacgga agataccgca gcagagcagg gcgaggtttt
     1801 tagggaagca gggaaaaaca gcgtcgcatc ccataatcct gcaaaccatg attcggaaga
     1861 atcttttaca gggaatatcg aaaatgagga aaatctcgtt tgctcaccgc tggtcggcac
     1921 gttctatctg gcaccgtctc cggaagaaga tccatttgta aaaccgggag atacggtgac
     1981 aaaaggtcag acgatcggca tcattgaagc aatgaaactg atgaatgaaa ttgtggcaga
     2041 gtgtgacggt accgtagaaa aagtacttgc ggaaaacggt gagatggtag aatatggaca
     2101 gccactattc tgtattaaat aatatcgtct ttggaggaag aaaaaatgtc agtgatgaaa
     2161 gcaaaagaaa tcatggaagt gatcccacac agacagccga tgcttctgat cgatacgatt
     2221 gaagaactga caccgggcga gcatggaatt ggcaagaagt gtgtcagtta caacgaaccc
     2281 tattttgcag gtcattttcc acaggaacca gttatgcctg gtgtactgat gattgaggct
     2341 ctggctcagg tcggtgcagt tgtgatgtta tcgtgtccgg aatataaggg gaaaacagct
     2401 tattttggtg ccatcaacca ggcaaaattc aaacggaaag tggttccggg ggatgtcctg
     2461 atgctggaag tggaaatgat caagaagaaa ggtccgatcg ggatcggaaa agccgtggca
     2521 acggttgaag gggaagtagc cgtatctgca gagctgacct ttgcgatcgg ataaaaagga
     2581 gagaaagatg tttcagaaaa tattgatcgc caaccggggg gaaattgcac ttcggatcat
     2641 acgtgcatgc agagaacttg gcattcgtac cgttgctatc tattcacagg cggatgcgga
     2701 agcactgcac gctcagctgg cagacgaagc catctgtatc ggaccatctg ccgcagcgga
     2761 gagttatctt gatatggaac gtattttaag tgcctgtatt gcagcaaaag cagatgccat
     2821 tcatccggga tttggatttt tgtcggaaaa cagtaaattt gcagatatgt gcagaaaatg
     2881 cgggatcacc tttatcggtc cgtccgcaga ggtgatcgaa cgcatgggaa acaaatccga
     2941 agccagaaat actatgataa aagccggcat cccggttgtg ccgggaaccg aaagaccact
     3001 caccgatgcc gggaaagcaa aagaggcggc agatcagatc ggctatccgg tcatgataaa
     3061 agcggcactt ggaggcggag gaaaaggcat gcggctttcg cagacagcgg aagattttct
     3121 ggaaaatttc aaggtagcac agctggaagc aatccgcggt ttcggagacg attccatgta
     3181 tatcgagaaa tatatcgaag agccgaaaca tattgagttt cagattcttg ccgataaatt
     3241 cggcaacgtc attcaacttg gggagcgtga ctgttccatt cagagaaatc atcagaaagt
     3301 gattgaagaa tctccaagca gcgtgctgga tgaaagactg cgaaaagaaa tgggagcggt
     3361 ggcagtgcgt gccgcaaaag cggcagctta cgaaaatgcc ggaacgattg aatttcttct
     3421 ggataagcag aagcgttatt attttatgga gatgaacacc cggatccagg tagaacatcc
     3481 ggtgaccgaa atggtatctg gcgtggatct gatcaaggaa cagatttata ttgcagccgg
     3541 tttgccgctt gatctgacgc aggaagagat cacgttacgc ggtcatgcac tggaatgtcg
     3601 catcaacgcc gaagatccaa agaagaattt tatgccgtgt cctggaaagc tcggtcagat
     3661 acatttgccg ggtggctttg gcgtgagggt tgatactgct atttacggtg gatgtgagat
     3721 atcgccttac tatgattcaa tggcggcaaa agtgattgtt catgac
//
LOCUS       DAWDWH010000207         3760 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_9118_length_3760_cov_8.174899, whole genome shotgun sequence.
ACCESSION   DAWDWH010000207 DAWDWH010000000
VERSION     DAWDWH010000207.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9118_length_3760_cov_8.174899"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2105..2114
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2465..2482
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1046 a          837 c          828 g         1021 t
ORIGIN      
        1 tgctatgata tgatgggagt tcggtgcatt cctgcttctg atggcgggta tttattttac
       61 tgcctataac ctgaattatg gctatccact gttcagttat acgctctctt ccatcacctt
      121 tctgtttctg atcgtgtcac cgatccttgc catgcgaacg cttgcagagg aacgcaaaca
      181 gaagacggac cagctgcttc tcactgcacc ggtgacggta tggcagattg tgcttggaaa
      241 atacctggca ctgatcgtca cttttcttct tccggtcgtg atcctgtgtc tctatccgct
      301 gatcatggca cagtacggaa gcattgcttt tgcatctact tacgtggcac tgcttggttt
      361 ttttctgctt ggatgtacct gcctggctgt gggactgttc ttctcgtctc ttacggaaag
      421 ccagattctt gccgcagtcg gaaccttcgc ttttctgctg gtatgttatc tgatggatgg
      481 aatcagcgga tttttcccgg aaactgccgg tgcttccttt tttgcacttg ttgtcattgt
      541 tctgcttata ggtttgctga tttatcatac cacacagaac atcctgcttc cgcttgtgat
      601 cggtgtcatc ggtgaaggtg tccttgccgt tctttatttt gtgaaacaaa gcatgttcga
      661 aggcatcatc cagaaaatac tgaatctttt caatatgtcc agtcacttca gcaattttac
      721 gaatagtatt cttgatctta ccggtgttgt ctactatctt tcctttattg gaattatgct
      781 gtttctgacg gttcagagca ttcagaaaag acgttggaat taggaggttg tcgccatgaa
      841 catcaattta aaatcaaaaa agccggctaa tatgctgcgg ccaaaaaaca aaaaactttt
      901 aaagaaggga tcttacagcc ttgcttttac ggttgtcatc cttgcgatcg ccgtctttct
      961 gaatcttctg atcggtgagc ttccttcctc tgcaacgaaa atagatgtca gtgaaaataa
     1021 actttacagt atcggtgacg agacaaagaa cgttgcttct tctctgacag aagatgtcac
     1081 cctttatctt cttgcagaag acggaagtga ggatgctaca ttaaacgaac ttctgacacg
     1141 ttatgcagat ctctcttctc acattaaata tgagaaaaag gacccggttc tgaatccaag
     1201 ctttgcttct tcctataccg acgaagatct tgcttccaac agtgtgatcg ttgtcagtga
     1261 caaacgaagc aaggtaatcc cttacagtga tctgtatgaa agcagtgtca attaccagac
     1321 ttactctatg gaaacaactg catttgacgg tgaaggtcag gtcaccagtg cgatcgccta
     1381 tgtcacaaca gaggatcttc cgattctgta tacattaaca ggacatgaag aaaatgatct
     1441 tgattcctcc atgcagtctg ctatcgaaaa agaaaatatc gaagttcagg aactgaatct
     1501 tctcacagcc ggaacggttc cggaagatgc atcctgcctg atgatcaatg ctccgcagaa
     1561 agatttctcc gacgatgaga ccagtgccat tctgacctac ctgcaaaatg gcggtcatgc
     1621 ttttctggta tccggatata ccacaacgga aatgccgaat tatcattcta ttctggaaca
     1681 gtatggcgtt tctattgaaa aaggcgttgt tctggaaggt gacaacaatc attatgtttc
     1741 cggaaatcct ctttatctgg tgccggagat ccagtcctgc gatgctaccg gaagcctttc
     1801 cggctcaaac agctatgtgc tgatgccgat cggtcagagt atcaaaacac tggacagcaa
     1861 atccgatgac atcacagtaa ccgatatgct gacttctacc agttctgcat atttaaaagc
     1921 agatccggaa aatatggaga gcctttccaa agaagagaac gatgaaagcg gtccgtttac
     1981 tctggcagca gccattaccg aagccatcgg aagcggatct gattccgaag aagagaccga
     2041 aacagaaact attgtagaag gagaaagcag tgcttctcag gttgaggctg aaagcgaaac
     2101 agaannnnnn nnnnctgttt ccggcggcaa tacaaaactt ctcacaaacg ctttaagctg
     2161 gatgtgcgga cagagttcca gtatctccat tccttccaag agtatgcagc tgtcttatct
     2221 gaccctgaca tctgcaaatg tcaacctctg gagcattatt accattgccg ttcttccggt
     2281 tctgttcctg gtaaccggcg gtgtgatctg gctgaaaagg aggaaaaaat aatgaagaaa
     2341 aaatcattcg gactggctgc ggccgttgct gttctgatcg tacttggcgg cagttatgca
     2401 ttgcttgcca accataaccg gatacaggag tcggaatcag ctgctgcgga atcggaaact
     2461 ggtgnnnnnn nnnnnnnnnn nnagcaacga tcgcctctga taaagtctct tcttttcagc
     2521 tggcagacgg tgatacttct tttacgctga caaaaacaga caacagctgg atctgtgttc
     2581 aggacgagac gtttccgtta tccaacgatg ctgcttccga actggtaaat accttcactt
     2641 ccctccaggc tgtgcgtgat cttggcactg cacaggatgg ggaaaattat ggattttcgg
     2701 aaaacgctcc tacggtcacg ctcacttctt ccgacagtac aaactgcagc tttgttcttg
     2761 gcgaagccaa cagcatgacc ggtgattatt atctgaaacg tgaagacagc ggagaaattt
     2821 ataccgtaag ctcttccctt gcagattcct ttcataaaac gttaacggat ctggctgaaa
     2881 tcgaaagttt tccgacgatt tcttccgata atatccagga gctttcgata accgaagaag
     2941 gtacgacact gctttttgag agcagtacca ccggttcggt aaaaacctgg cagatttccc
     3001 agtctcagga cggaggcaaa acagaaagta ctcctgaaaa agcgaccgta aacaccgtga
     3061 caacccttgt ggaccacctc ggaaatcttt cttttgacag ttttgtggat cataaagaag
     3121 aaaacaaaga aaaatacgga ctgaagaatc cgtccatgac gataaacttc caatataccg
     3181 aagcgacaga ttcggatgat gctacaacgg aagccgagtt agaaacagac ccttcttccg
     3241 aaacagaaag cgaaacagca aacgaaaccg aacctgccga aattctcagc ggaactctct
     3301 atgtgggaga acaggcagaa gacggatctt actatgttca gctcgatgga agcaataata
     3361 tttatctgat gtcagcggat aaactgtccg cattccaggg attaaattac gatgattatc
     3421 cggagagtga agaagaaagt acagaaagtg aaacctccgc cgagtagaaa tttactgttt
     3481 tttctttttc agtacaaaaa gatgaaatga aagtgttata gagtgccgga cgcaaaaaga
     3541 cagggcacaa tttacacttc tataatcttt ttttgttttg cacagaagaa agaaatagcc
     3601 tccataccgt ctgcccggca gtttacgcta tggaagcgaa agtgaattca gcaaaataat
     3661 tttggcttga gaaagctttt tgcttaagcg acccctggca ggccaggagc ttttagcaat
     3721 gtgcaaacga aaccgcattg taaaacatag tgcgtaaaat
//
LOCUS       DAWDWH010000208         3592 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_9566_length_3592_cov_16.030534, whole genome shotgun sequence.
ACCESSION   DAWDWH010000208 DAWDWH010000000
VERSION     DAWDWH010000208.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9566_length_3592_cov_16.030534"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1272..1281
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1259 a          619 c          882 g          822 t
ORIGIN      
        1 gtgcggcaaa gatggcaggg ttaaaagagg ttccggctat tatcaagagt tatacagatc
       61 gggaaatcct ggagatttct ctgatcgaaa atattcagcg ggaaaattta aatccgatcg
      121 acgaagcaag agcttataaa aagctgctca ccgagtttca tctgaagcag gatgaagtgg
      181 cggagcgtgt ttccaagagc agaacggcag tgaccaattc catgcgtctt ctgaagctgg
      241 atgagagagt acagcagatg gtgatcgatg aaatgctcac gaccggccat gcgagagcgt
      301 tgatttctat tgaagaccga gatctgcagt tccagatggc aaatcagatc tttgataata
      361 aattaagtgt cagagagaca gaaaaactgg tcagaaagct gggcgaagaa aaggaagaaa
      421 ccacaaaaac aaaagaaccg gatgtagagg tcatttaccg gaatctggaa gaaaaggtaa
      481 aagaaatact cggtacaaaa gtacagatca gtcacaaaaa gaataatagc ggaaaaattg
      541 agattgagta ttattccaat gaggaactgg agaggatcat tgaactgctg gaaagcgttc
      601 agaatcatta aaattaaata aggaatggag ggaaaatatg gggatttttg atgcgatagg
      661 cattgatgca ggtatcgtgc tgatcattgt aattctgata cagcttgtgc tgattttttt
      721 cctggtcaag cagtccatga agctggcaag acttacgaat aagtacaaaa tgttcatgcg
      781 tggaaaagat gcgatatccc tggaaaaagc atttgaaaag aaattcgaag aagtagatgc
      841 catgacagaa gcagtaaaat atcaggcgga tgagatttat aaattaaaag aaatccagaa
      901 aatgacactg aacaaaactg caatcgtaaa atatgatgca ttcaaagatg tcggcggcaa
      961 attaagtttt gcacttgcaa tgctggataa agagaacagc ggatttgtat taaatgcgat
     1021 ccacagcaga gaaggctgtt atacttatgt caaagaaatc gtaaaaggcg agtcttatat
     1081 cgtgcttggc gaagaagaaa aagaagcact gcgtcaggct gtaaatgcac acaacgatat
     1141 cggataacgt caggtattaa aacagaaagt gtttgcacag aaggacacaa agtcacttta
     1201 cgaggcagat tacatgtgtt aaaatcggaa aagaagtgac ccgtgatcca gagggtcttg
     1261 agttgaaata annnnnnnnn naaaatgaaa taaaagaaaa ggacgataaa aaatgttaga
     1321 cattaaattt gtgagagaaa atccggaaat tgtaaaacag aatatcagaa ataaattcca
     1381 ggataaaaaa ctgccgctgg tagatgaagt actggagctg gacaagagaa accgtgagat
     1441 caaacaggaa gtagaagctc tgcgtgcaga aagaaacaaa ctgtcaaaag agatcggcgg
     1501 cctgatgaaa cagggtaaaa aagaagaggc agaggaagta aaacgtcagg tagcagcaag
     1561 tgctgaccgt atcaacgaac tttccgcaga agaagctgaa gtagaagaaa aactgaaaac
     1621 aaacatgatg gttatcccga acatcatcga tccgtctgtg ccgatcggaa aagatgacag
     1681 cgaaaacgta gagatcgaac gcttcggtga gccggttgtt ccggattttg aaatcccgta
     1741 ccatacagaa atcatggaat ctttcaacgg tatcgacctt gacagtgcaa gaaaagttgc
     1801 cggaaacgga ttctattacc tgatgggaga cattgcaaga ctgcattccg cagtgatcgc
     1861 atacgcacgt gatttcatga tcaaccgtgg atttacttac tgcgtaccgc catttatgat
     1921 ccgcagcaac gtagttaccg gcgttatgag ttttgccgag atggattcca tgatgtacaa
     1981 aatcgaaggc gaagatctgt atctgatcgg aaccagcgaa cattctatga tcggtaaatt
     2041 catcgacaca atcattccgg aagaagagct gccgaagacg ctgaccagct attctccatg
     2101 tttcagaaaa gaaaaaggtg cacacggcat cgaagagcga ggtgtatacc gtatccacca
     2161 gtttgaaaaa caggaaatga tcgttgtatg caaaccggaa gaaagcaaaa tgtggtatga
     2221 aaaactgtgg aaaaacaccg tagatctgtt ccgcagcatg gatatcccgg ttcgtacgct
     2281 ggaatgctgt tccggtgacc tggcagacct gaaagtaaaa tcagtggacg tagaagcatg
     2341 gtctccgaga cagaaaaaat atttcgaagt aggaagctgc tcaaacttag gtgacgctca
     2401 ggcacgccgt ctgaaaatcc gcataaatgg tgagaatggc aaatattttg cacatacatt
     2461 aaacaatacc gtagttgcac cgcctcgtat gttgattgca ttcctggaga acaacctgca
     2521 ggctgacgga agcgttaaga ttccggaagt acttcagcca tacatgggcg gcatgaaagt
     2581 gattgaaaag aaataataga ggtggcataa ttgcataatt atctgcgagc aatcggattt
     2641 agcaatctgc agaatgaaaa agatataaaa gaaatcctca ccgaggtttt ccacgatttt
     2701 gacgaaagag aagtttcaag agaagggaaa aacaaagctt ttgtagagta tacaaaaagc
     2761 tttggtgaaa atatgggaat caagatgtgc ggcatcatgg atacggatgg tttccatcag
     2821 gagtattatt tcccatattt tcagggaaaa gatatcagca gcaaagaaga tctgatcatt
     2881 gaacgccatg cggcaagaga atcctttgcc ggtgtctgcg aagatgtaag gatcggtgtt
     2941 tcggttattt tctatttaca gaatgccgca aaatataaaa aggaaatgct gctcggacat
     3001 ctgttgagtg ataagatcag cacgagcttt tccggacttt ccctgaaagg caaaatttta
     3061 tttccagtac agaaggcgga gccgcgtgtc actgcgacag gaagtgattc ggcaaatcag
     3121 aggcacaaaa tgatcgcagc cgcaagacag ggtgatgcag aggcaatcga aagcctgaca
     3181 ttggaagata ttgatactta tgcggcagtc aatcagagga ttttaaaaga agatcttttc
     3241 agcattgtag acacactctt tatgccgtat ggcctggaat gtgatcatta tcaggtcatg
     3301 ggaaacatta aggatgttga aaaaacagtg aataaatata caaaagaaac catttatcag
     3361 ctcaggctgg agtgcaacga tatgaacctg gatgtctgta ttaacaaaga ggatctgctc
     3421 ggtgagccgg aggtcggaag aagatttaaa ggtactatat ggcttcaggg acatatcaat
     3481 ttcgcaaatt aagtgtggac gcataaaaaa ggctgtgcta taataaaaaa gctggcagct
     3541 ttggctgcca ggcacactgt ttccttagtt aaatggatat aacaagtgtc tc
//
LOCUS       DAWDWH010000209         3557 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_9677_length_3557_cov_10.859509, whole genome shotgun sequence.
ACCESSION   DAWDWH010000209 DAWDWH010000000
VERSION     DAWDWH010000209.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9677_length_3557_cov_10.859509"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1049 a          758 c          802 g          948 t
ORIGIN      
        1 gtcgatggag ttgtgatcgc ctgtatcaac ttttttaccg aagaagttca acggctggta
       61 caatccgaga ttccagtggt cacgattgat catgtattcg atggaagaat tgctgttgtt
      121 tccaacaaca ttcagggaat ggaagatctc gtctcctata ttctgaaacg ggggcacaga
      181 aagatcgcct atattcacgg agaagattcc tccgttacgc gaagccgact cggaagtttt
      241 tacagaactc tccagaaaaa cggattgatg tacctgatga atatatgccg gtcatcccct
      301 atcgcgatgc ggaagcggca gcgatggcaa cttcagaact tcttgatctg aaagatccac
      361 ccacctgcat tctatatccg gatgattttt ctgctctggg cggtatcaac gagatccatg
      421 aacgtgggct ccggataccg gacgatatct ccattgcagg atatgacgga ctcacttttg
      481 cacgcatcct ggagccccgg ctgacaactc tgtgtcagga cacagaaaca atcggacggc
      541 ttgccgcaca gaaactgatt gatctgattg agaatcccaa aactacgatc atcgataagt
      601 tcaccgtaga tggaacactc ttcccaggag catcagtcag aaatctggca aaataggaaa
      661 ttaaatatgg aaagaatata aaacaaaaac tatttatcac agggaggtaa caacatgaag
      721 agaaagatta tggcagtcac attaacagca gctatgcttg caggtctggc agcagttccg
      781 gtatgggcag atgacacagg ttccgatgaa ggtaaagttt taaacattta ctgctggaat
      841 gaagaattca aatcccgtct gaccgatcat tacccgggat acgaagaagt agatggaacc
      901 catggcaaga tcggtgatgt agatgttgta tggaacatca ctccatctga tgacaatgca
      961 tatcagaata acctggacga aacacttctg aaacaggctg atgcatcagc agatgacaag
     1021 attgatctct tcctggtaga agctgactac gcactgaaat atgtaaatac cgattacaca
     1081 atgccggtag cagatctcgg aatcacagaa gacgaagttg ctgatcagta tcagtataca
     1141 aaagatgtta tgacagatgc tgatggaaat ctgaaaggtc tttcctggca gggctgccct
     1201 ggaaccatga tctacagaag agatatcgcc aaagaagtat tcggaacaga tgatccggca
     1261 gaagttcaga agaaagttgc tgactgggat accttcaaag caacagctga agaactgaaa
     1321 gaaaaaggtt atcagatgac ctctactgta aacgacagtt accgtgtatt ctccaacaac
     1381 gtatctactc cgtgggtagt tgatggaaag atcacagttg atgacaacat caagaactgg
     1441 gttgatatgt ccaaggaaat ggttgacgca ggtgaaactg ctacatacga actttggagt
     1501 gatgactgga gcaaaggctt cttcaaagac agtaacgtat ttgcatactt cggaccagca
     1561 tggttcatcg acttctctat gagtgctgat caggatggtt ctgttggtaa agacggcggc
     1621 tgggctgcaa cagaaggacc acagggcttc tactggggcg gaacatggat cacagcagct
     1681 acaggaacag ataacccgac actggttgct gatatcatga gaactatgac aactaacgtt
     1741 gacgttatga aagaaatcgt tacagctgat aatgacttca taaacaacaa accagctatg
     1801 gaagaaatgg cagcagacga aagctacggc gatgctgttc ttggcggaca gaacccactg
     1861 gcaatgttct gtgccggtgc agacaagatc gacctcagca atatgagcat ctacgatcag
     1921 ggctgcaacg aagaattcca gaacgctatg aagaactact ttgaaggcaa cgcaacatat
     1981 gatgaagctc tcgacctgtt ctacaaagca gttgtagaaa aatatccaga actttcttac
     2041 taatcaaaac agacagattc tgttgaataa ttattgagta aaaagatgtg cctgcctgac
     2101 catcaggcag gcacatcttt cgattatgga ggaagataat ggcaaagaat aataaccata
     2161 aagcggtcag ctataacaaa tggggatata tcttcctgat accgtttgta gtagtatatc
     2221 tcatattcca gctcattccg cttattagta cagtttataa cagcttcttt gaaaactaca
     2281 gatctggatt gacgcagatt ggaccgaact ttgtaggctt tgccaattat gtcaaactct
     2341 tcgagggtgg agagatctgg atctatttta aaaacactat gatcatgtgg gttatgggat
     2401 ttgttcctca gatcctggtc tctctgctcc ttgcagcatg gttctccaat ccgagtcttc
     2461 gacttaaagg tcagagattt tttaagaccg taatttacct tccaaacctg atcatggcat
     2521 ctgcttttgc aatgttgttc tttacattat ttgcagacgg cggcccgatc aatgcaatgc
     2581 tgatacaggc aggaatactg aaacaggctt actccttctt cagtcatgcc tggagtgcca
     2641 gaacgcttgt tgcagtcctg aacttcctta tgtggtttgg taatacgaca atccttctta
     2701 tggccggcat gatgggtatt gatccatcac tttatgaggc agccgaagta gatggagcca
     2761 ctgcttcgca gattttcaga aagatcactc ttccgcttct tcgtccgatt ctgatctatg
     2821 ttatgatcac atctctgatc ggtggtatgc agatgttcga tgtacctcag atcctgacaa
     2881 acggaaccgg tgatccaatg cgttccacta tgacactgat catgtatctg aacaagcatc
     2941 tgttcagcaa gaactatgga atgggcggtg cattatccgt attcctgttc atcattactg
     3001 gcgtgctcag tctgattgta ttccgcttta acaagacgaa tgacgggagg tgagaaggat
     3061 gaatgaaaaa gaccaaaaaa cccaaaaagg aatgagcatg aaaggtcagc gtactcttgc
     3121 ttatatagta ctgatcatta tctccttctt ctgcctgttc tggttttatg tactgtttat
     3181 caatgcgacc agatcaaacg gtgcactgaa aaaaggtttc acagcgatcc ctggtggatt
     3241 tcttatcaaa aactggaaga gcctgcttgc cggaacactt ccggtatgga atggtatgtt
     3301 caacagtatt ttcattgcta cctgttctgc tgttttatgt acctatttct ccaccatgac
     3361 tgcatatgcc atccacgcat atgattttaa aggcaaaaaa tttatgttca cttttatcct
     3421 tgcagtcatg atgattccta cgcaggtaac tgcactcggt ttcctgcagc tgcttggtaa
     3481 gatgcatctg gacgattcct ttattccact gatcgttccg agtatcgcag ctccggttac
     3541 tttcttctat atgaaac
//
LOCUS       DAWDWH010000210         3502 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_9839_length_3502_cov_12.903394, whole genome shotgun sequence.
ACCESSION   DAWDWH010000210 DAWDWH010000000
VERSION     DAWDWH010000210.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9839_length_3502_cov_12.903394"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2837..2846
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3146..3181
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          792 a          889 c          695 g         1080 t
ORIGIN      
        1 actgcggagc agttatttgg ttatgagcaa gtagcaaagc ggattgcgaa tatccacttg
       61 actcagttga gtactttgcc tacaacgctt tagttctttc cattaatata ggaaataagg
      121 ccttctgctt tgatgatctt ctgcataaac tccgggaacg cctgtccttt aaactcggtt
      181 ccttttgtgc gattatagat catgccgctg tcaaaatcta cttctacgtc atcgccgtcc
      241 tcgatgcctt tggatgcttc cggacactcg atgatcggca gtccgatgtt gatcgcatta
      301 cggtagaaaa tacgtgcaaa ggtctctgcg atgacacagc ttacacctgc tgccttgatg
      361 gcgatcggtg catgttctct ggaagatccg cagccgaaat ttttctcagc tacaatgata
      421 tctcccttat gaactttgtt tacaaactct ttatcaatat cttccatgca gtgtgttgcc
      481 agttctgccg gatcggaaga attcagatat cttgccggaa tgataacatc ggtatctaca
      541 ttgtctccat atttaaatac tttaccatgt gctttcatct tatctatcct cctataatcc
      601 aagctctgcc ggtgcggaaa tctttccggt cactgcactt gctgctgcaa ctgccgggct
      661 tgccagatat acttctgatt ttacatgtcc catacggccc acgaagttac ggttggtagt
      721 ggagatacaa cgctcgccct ctgccaggat acccatgtag ccgccaaggc atggtccgca
      781 ggtcggtgtg ctgacaactg ctcctgcttc gatgaagatt tccagcagtc cttctctcat
      841 agcctgcaga taaatctgct gtgttgccgg gatcacgata cagcggacac cttttttcac
      901 tttgcgtcct tttaaaatct ctgctgcaca acgcagatct tcgatacgtc cgtttgtaca
      961 ggaaccgatc acagactgat cgattacaac atcttctgtg atctcatcga tggtcctggt
     1021 gttttccgga agatgcggga atgctacggt tgatttcaga gtgctgaggt caatggtata
     1081 ttcctgttcg tatacggcat cttcatctgc ttcatagact ttatatggac gtttggaatg
     1141 ttctttcata tatgcaacgg tcaggtcatc taccgggaag ataccgttct tgccaccggc
     1201 ttcgatcgcc atgtttgcga tcgtgaaacg gtcatccatg ctcaggtatt tgattccttc
     1261 accaacaaat tccatggatt tgtaaagagc tccgtccaca ccgatcagtc cgatgatgtg
     1321 caggatcaca tctttgccgc ttacccattt tgccggtttg ccgaccaggt taaatttaat
     1381 tgcagacggt actttaaacc atgcttttcc ggttaccata ccggctgcca tgtcggtact
     1441 tccgacaccg gtggagaatg ctcccagtgc tccgtatgta caggtatgag aatctgcacc
     1501 aatgatcaca tctcctgcca cagtcagtcc tttttccgga agcagtgcgt gttcgattcc
     1561 catctctcct acgtcaaaat aattggtgat ctcattttta caggcaaatt cacgtacaca
     1621 tttgcagtgt tctgctgatt tgatatcttt attcggaata aaatgatcca ttaccagtgc
     1681 gatcttatcc ttgtcaaata cttcctgttt tttaaacttg tccatctcat gaatggcaac
     1741 cggggaagta atatcatttc ccaggaccag atccagatct gcctcgatca gctgaccggc
     1801 ttctacactt ggtaatcctg catgagctgc aagaattttc tgtgtcatcg tcattcccat
     1861 gtcttgtttc ctcctcattc tcgtgtttca tgcagaaact cttttctttc tgcatactct
     1921 ggcattgaaa taccgccttt tgcggatttt aaacctgttt tttatcatag cataaatatt
     1981 ggtaaattac aacaaaactg ccgtgtattt tatgggcaaa acttttatct gtaaaaaaga
     2041 tgccacccgt tattacaggt gacacctctc ttctatactt atctcaaaac atctgcaaat
     2101 gctctgtcta aaaagacacc acaaacatct tatttattgc tcaggaattc atggatacct
     2161 tttgctgctg ctttacctgc acccatagcc aggataacgg tagctgctcc ggtaacggca
     2221 tctccacctg cgtatacacc atcttttgat gtctttccgg tttcttcgtc cgcaataata
     2281 cattttctct tattgacatc cagaccgatg gtcgttgaag aaatcagcgg gttcggtgaa
     2341 gttccaaggg acatgataac ggtatctaca tccaggacaa attcagatcc cgggatctct
     2401 accggtcttc ttcttccgga agcatccggc tcgccaagct ccatacggat acatttcatg
     2461 ccggctactg catcgttctc atctaccagg atttccgttg ggttggtcag cagatcaaag
     2521 atgatacctt cttcttttgc atgatgtact tcttctgctc gtgcaggaag ttctgcttca
     2581 cttcttctgt atacaatatg tacttctgca ccgagacgaa gtgcggttct tgcagcatcc
     2641 attgctacgt ttccgccgcc gacaacagct actttcttgc catgtacgat cggtgtatcg
     2701 taatcttcac ggaatgcttt catcaggttg tttcttgtca ggtactcgtt tgcagaaaat
     2761 acaccttttg cggtttctcc agggattccc atgaacatcg gcagacctgc accggaaccg
     2821 atgaagactg cttcaannnn nnnnnnatac cagtacaccg cctgcctcat gcagtgcttc
     2881 aaagatggtt acgtcataac ctaattttgc cagatcacct gcacaggtca gaccggatgg
     2941 gccggacccg ataacagcta ctttcttgcc gttcttttct gccggagctg ccggacggat
     3001 gccgttctct cttgcccagt cagctacaaa acgctccagt tttccgatgg aaatggcttc
     3061 gcctttgata ccgcggatac attttccttc acactggctc tcctgcggac atacacggcc
     3121 gcagattgct ggaagtgcac tggacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 ngatctgata tgctttttcg aagtttcctt cttttacttc atgtacaaat gccgggatat
     3241 tgatggatac cggacatccc tgcatacatt ttgcattttt acagttgatg cagcgggaag
     3301 cttcttccat tgcttcgtct gcattatatc caaggcaaac ctcatcaaag ttggttgctc
     3361 ttacttttgg tgcctgttct cttacaggaa ctttctttaa tacatctgcc attatttgtc
     3421 acctccgcaa tttccacatc cgccatgatg tgtgtcacct tcacgcagtt tcagaagtgc
     3481 acgtccctct tctgtcttgt cg
//
LOCUS       DAWDWH010000211         3496 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_9850_length_3496_cov_8.348736, whole genome shotgun sequence.
ACCESSION   DAWDWH010000211 DAWDWH010000000
VERSION     DAWDWH010000211.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9850_length_3496_cov_8.348736"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1031 a          704 c          863 g          898 t
ORIGIN      
        1 gactctgtcc cggtttcctg cggtttaaaa atttttggat atcgtcctcg caaagcgaaa
       61 gaccggcagg gcatccgtcc acaacgacac cgacgccggc accgtgagat tcgccccatg
      121 tagttatttt aaataaagtt ccaaatgtag aacctgccat aaaattacct cctaagtaaa
      181 ataagatgtc gggaccaaaa gacagctgcc ccataccgga tgcctgaaaa cagttctgtt
      241 acagagttac agagaaattt tcttactttt aaatatccca tatcatcatt ttgaggttat
      301 tataatgaaa gatttcagaa aattcaatag aaagttggaa atagtggaaa gccttggagg
      361 ctatcgtttg ccgaatatct gtgaacagca gcaggctgtg cttcatataa tagagcgagc
      421 agaaagcaat cgtggagctg acagagaatg gacaatgcaa aaacatgtcg ttttggattt
      481 attcatgtga tcgagcaggg agatacttta tataaactag gaaagagata tggtgtaacc
      541 gtttcggccc ttctgtttgc caatccctgg gtaaacgtat acaatctgca gatcggagat
      601 gaactgtgca ttccggattt cggatgccga agatgcaaag agacaaaagg ggaaaatttg
      661 gatgagaaaa gagcggatga aatgcagagc ctttcataat ctaaagtgtt tgtaaacttt
      721 gtgaaaaatc aggcactttg attgacaaaa gatacaatca aatttataat agattcggca
      781 ttgacaggca ggcgggacag agcatttttg tcccgtgaaa gcttgcagaa cacacgatgc
      841 ggaaaggaag atgaaagaac ttttatgaac tttttaaaca aaatggaacg taaattcgga
      901 cggtatgcca tacacaatct gtccacgtac atcatcggag cttatgttat cggttatttt
      961 ctgtatttcc tggcaccggg tgtactgtat aactatttaa gccttgagcc gtattatatc
     1021 ttaagagggc agatctggag actgatcacc tgggttttaa ttccgccgga atcacctggt
     1081 atttttacga tcatcatgct gatgctgtat tattctcttg gcaattcact ggagcagacc
     1141 tggggggcgt tccggtataa tgcttatatt ttttcaggaa ttatttcgac aattatcgga
     1201 gcatttatct tatatgctgt gatgggcgga aatatcgtgt tcgggcaggc actgttttcc
     1261 acatattata tcaatatgtc catttttctg gcgtttgcag tctgctatcc gaatatggag
     1321 ttgctgttgt attttatcat accgatcaag gtcaaatggt ttgggattct ttacggagct
     1381 ttcattgtcc tttccttcct tcagacaaac tgggctggaa gagtggcgat cattgcatcc
     1441 atgttcaatt tcatcctgtt tttcctgatg acgagaaatt acaacaaggt ttcaccaaaa
     1501 gaaatccgtc ggaagcaaaa tttcaaaagg cagaccagtc agtcaggcag atccggtatt
     1561 accaagcaca agtgtgcgat ctgcggcaga accgagctgg acggtgatga tctcgaattt
     1621 cgtttctgtt ccaaatgcga cggaaattat gagtattgcc aggatcatct gtttacgcac
     1681 aaacatatca aaaaataaaa atgtggtcag gccgcaaagc ggcagcagga ggaataatga
     1741 agaaaacaaa gattatctgt acaatgggac caagaacgat ggacaagatc attctcaaag
     1801 aactgatcgc aggcggaatg aacgtggcac gtttcaattt ttctcatgga agctacgaag
     1861 aacatgctga gcgtatcgca ctggtgcgtc aggtttccga acagcttggc attccggttg
     1921 cactgatgct ggatacgaaa ggaccggaga tccgtaccgg gcttctcaaa gacggcaaga
     1981 aagtatctct ggagcaggga aaggaattta cactgtatac agaagagcga gaaggcgatg
     2041 aaacaggatg ttccatcacc tatcagcagc ttgtctacga tgtgagaaag ggtgacacga
     2101 tcctgatcga tgacggtctg atcggtctgg aagtacagcg tgtttctgcc gacaagattg
     2161 agtgtatcat caaaaacggc ggtgagctgg gtgagcgaaa aggcgtcaat gttccaaatg
     2221 taaaaataca tcttccgggt gtgacacaga aagacagaga agatattctg tttggtattg
     2281 aacagggtgt ggattatatc gcagcctcct ttgtacgaaa cagtgactgc atcatggata
     2341 tccgcgagat cctggaagat aatcacggca gagatatcgg cattattgcc aagatcgaaa
     2401 atgcagaagg tgtggaaaat attgatgaga tcctggatgc ggcagacggc atcatggttg
     2461 cacgagggga tctcggcgtg gagatcccgg cagaccaggt tccgcatatc cagaagaaga
     2521 tcatccacaa atgcaaccgc aaatgtaaac cggttgtcac tgcaacacag atgctggatt
     2581 cgatgatccg caatccgcgt ccgaccagag cagaggccgg tgatgtggca aatgcgatct
     2641 acgacggcag tgatgcgatc atgctttccg gagagaccgc aatgggcaaa tatccggtag
     2701 aagcagtccg catgatggca aagatcgcag agacgacaga agctcatctg gattactcca
     2761 atcttcagaa attaaataaa aaacagcgta aaaaagatat ttccatggca gttggatttg
     2821 caagtgtgtc cacagctgag atccttaaag catcctgtct ggttgttccg accatgaccg
     2881 gctacacggc aaggatgatc tccagcctgc gtccgaagac accaatctat gcgatctctc
     2941 caagtgagca ggcagtccgc aggatgcagc tttactgggg tgtctatgcg atgccgggtg
     3001 aaacagaaga cagcacaagg catatgatca ccaactccat gaagattatt ttacgccgca
     3061 aactgattaa gaaaggagaa ctggctgtat ttacagcggg cgatccggca accaacatgg
     3121 tatctggccg tggacgttcc acaaatgtaa tgcaggtggt tgaggcaaaa taggcgatga
     3181 caaagtcgaa gaagagaaga ggagttgcac ttggtgcagc tcttcttatg attattttag
     3241 aaatactggc agtaaaaggc gatggatttc agctggcaaa agcaggactg gcactgctcc
     3301 ttgcaatcct ggcgggcggt tttatcgcct gggattttaa aataccaaag aaattaagcc
     3361 cgatcgtgtt tctggcactt ccggcggcag ccttgtgctg catggagttt tttacccatg
     3421 tgccatggga tctgacaccg ttaatctttt ttttgaacta cctgttttat ctggtgttat
     3481 atctgatcgt aacagc
//
LOCUS       DAWDWH010000212         3489 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_9870_length_3489_cov_10.636867, whole genome shotgun sequence.
ACCESSION   DAWDWH010000212 DAWDWH010000000
VERSION     DAWDWH010000212.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9870_length_3489_cov_10.636867"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1182 a          587 c          845 g          875 t
ORIGIN      
        1 cgtatatgga tttgagcaga tcacagagat tgcaagaaac aacggaaata tttcctggtg
       61 gcttagaaat ccaggatttt cacagaattt tgccatgaat tttggatgga gggtgcatta
      121 tggcggactg caagtagatg aacctgccgt cggcgtacgt ccggcgatcg tgatccagac
      181 cagatagtaa atctgataat tgtgctccag accaggcagc agatccgatt tatcgtgatt
      241 cagatccgga ggcagatttg atgggagagg tgtatatggg aaaattaata ttgacagcac
      301 ggggacttaa ttccgaaaca gggcagaaaa tcatccgcaa gtgtctgacg gaagaagagg
      361 agcgggaaaa gaaaactatc cttctgatca gcatacaaga gtatagaatt gatgaaatcc
      421 tgaaacaggc aagtatggaa ctgggatttt tggaagaaca tatttttctt gcatccgggc
      481 agattgatct gaaaaaaatg tgaccggatt atatttacgt tactgaagga aatgtatatc
      541 aagtattaga ctatatgagg aaaaacgggc tgttgaaatt tattcaacaa caaatgaaac
      601 gaacagactg cacctatatc ggaagcagtg ccggtgcaat gattgccgga acagatatct
      661 atctggcaga agattttgat gaaaatattg tgggtttaac agatctgaag gcattggggc
      721 tgtttgatgg aagtgttatc ccacattact ccagacgaga tctgaaacgc tacatcaaaa
      781 attcgaatca gaaagatatc gggcgttaca gtgaactgta ttcagtggat gacaatgaag
      841 cgttagtggc ggaagattcc aaagtgaaaa aggtgaactt cagaaaagta aaagtgaaag
      901 aatagcaaaa atgagcatta tcttgagaaa tttctgtaaa ctgattataa taaaaattaa
      961 gaaacaggag atcaagataa tggttcaaga catagcagtt gaaaatgagg tgatgatata
     1021 tgtcaaagac tatggagaat ttaatagaac aatacgttga agctgttaaa aagatttatg
     1081 gttcacatat acgacagatc attctatatg gttcctatgc aagagaagat ttccgaccgg
     1141 attcagatgt agatattatg attttgctag atatgtctga tctggagtta aaggcatatg
     1201 cacaacagct ttcttatatg acctatgatt ttaatatgga caatgatctg gatattaagc
     1261 ctattgcaaa aagtgaaaca cattttaaaa agtggattgt aaattatccg ttttattcca
     1321 atattcataa agaaggggta gttttatatg aagcagcgta aaccggaaat tggtacaaga
     1381 cgggatttgg ttctatatcg tctggaaacg gcaaaaaatg atctgaaatc tgcaagagcg
     1441 ttatttgcaa tggaagacta tcgaggagca aataacagag catattaagc tatctttcat
     1501 gcgattaatg ccgtacatgc tattaatgga aaagcatata aaagacacaa agatgctgtg
     1561 gcgaatttca acaaggatta tgtaaaaaca tctgtatttc caaaagaaat gggacggaaa
     1621 attggacagg cggaagagat tcggcatgca agtgactatg atgattttta tattgcaagt
     1681 aaagaagaat cagaaaaatc cttattcagt taaaaaactg gatagggatt tttttattcc
     1741 ccctttttta gtcgaaaaag agtggaatta aaatattatt ataaaaaata ctaagaggag
     1801 gtatgaagaa aaataaaggt acaaagtccc ccatctgaaa gagattttcg aatcatttat
     1861 gattataagt aagaaaggag tggtgctatt gaaaagcaaa ataatggcag gtgttctgat
     1921 gtttattatc atctctgtgc ttctgaccgg atgtgggggg aagcaaaagg agattcctta
     1981 cccggatggc tattatgtac cgaagccgga ggagacggaa acggcaggaa agtccgataa
     2041 atcgcagaag aacgccagtg aaacggcaaa agagacaacc agggaaacga tccaggcgag
     2101 agccggaagt gaaacagaaa acaagaagat ttcgttttac ctggatacgt caggaagcat
     2161 gcagcgttca ccggaagtgg tgaagatcca tgccgccgcc acgaaatgtg ccgccggata
     2221 tgaggagcgt catttttaca gtgtggacag cagaaacctg atcgaaacaa cggagcagct
     2281 ggcactcagt gggcagtatg gaaaaggtgc cccgatcgac ctgattcggg atggaacgct
     2341 gccctgtgat ccggaaagtg tcaatgtgct gacgacggac cttcagacgg ggacaagctg
     2401 ttcggaaatc ggcaagtggc tggtgaatag cggggcgaca ggctattcgt tctacgtttt
     2461 tacgatgcag tacaatggca gtctccagtt caaaatgtac acttcaagca gtgtgcttga
     2521 aacagtttct gtcaaggatt gctcttttaa tcagaaagaa tttctgatga tagtctttgg
     2581 gaaaaacagc ctcgtagaag attacgatca gtttttccag tacaagctgg ggacggaagt
     2641 gccatatgat acctgccatg tttcccttca tgcagaggaa aaacaggaca gcaatttcct
     2701 tcagctgaca tcgagcaaat gtttcaagaa cgacattgcg aatgtaactt atgacaacac
     2761 caattatgtg tttggcatgt ctcttgttga tacggaaaat actgaattta cctgtccgaa
     2821 tacgtttgta tacagaaaga gcggaaactc gacaagtaag gccaagaaag cagttaaagc
     2881 aatcctgtat gcagtaccaa atcaggcagt tccggctgtt gaaaaggcag agattacgaa
     2941 tgtcatggag tatgacgaag aatccgggac atacaaagaa agcaatgtga cattcaagat
     3001 ggctaccaaa gcatatctgg gagcgttttc tgccatcgta aatgaagtga ctgacacaaa
     3061 gaagtaccag cgtctttata aagatctggg aggcgttatc gcccagggtg attcgatctt
     3121 taccgtttct cttgaaaatg aatcacttcc aaaaggactg tttgcagtcg aggtgcagct
     3181 cacgttcgca gcaaccggtg aagtggcgga tcttcagaaa tttgcagcaa atcacagtgc
     3241 aggattggaa gattacagca cggctctgaa aagtgagtgc acaccggaaa tcatcaaaaa
     3301 acaggaaagt acctcgaaat tttattatac aggagatgga agttcttcgt cggttttctg
     3361 caaactgctg gaatttgaaa agattacaga tgagctgatt gcagctggag ctgttgccga
     3421 gtcagacaac gagatgctta ccatccgact gatcatcgac aatcgctaaa gagcaggaga
     3481 atcaacaaa
//
LOCUS       DAWDWH010000213         3424 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_10085_length_3424_cov_9.393292, whole genome shotgun sequence.
ACCESSION   DAWDWH010000213 DAWDWH010000000
VERSION     DAWDWH010000213.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10085_length_3424_cov_9.393292"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1775..1784
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1082 a          658 c          863 g          811 t
ORIGIN      
        1 gtccctcctc caacggagag aagcgtattt ttcttccagc agtgtaccag aacgatggcg
       61 atgccggcga tcagctctgg cagtccgaac ggtgtctgca gcagggaaac attcttgaag
      121 cagtaaacca ccagcatacc gatgatagcg taaggaagta cttttccgag gtacatgatg
      181 taagcaggtg tcttggattt tccagaaaac aaaaggaacg gaagtccgcg tacaaaaaaa
      241 gtcagtaagg acatgatcgc gatcaaggcg attgcctgag aatccgggat actacgcatg
      301 tgaatcatct cctttctgat cttctctttc catatgctcc tccagcggct tacgaagcag
      361 tgtcaagatc gtaagaatgg agagcatggc aggaatgata aaacggtccg gcccgaaaag
      421 aagcagacag atcacagagc agatcacacc ggtgattgcc gggatatgat ttttgctgtt
      481 cagccactga tcagtaaaga ttacaataaa cagtgctgtc atgacaaaat ccacaccttt
      541 tgtattgaag ttgaccaggg aaccaaagag tgcaccgaac gtgcagccga tgatccagta
      601 aacgtgatcc agcatggaga taaacaaata gaaccatccc ttgtccacac cgtccggaac
      661 cggggttgta gcaaccagag aataggtctc atcggtcagc tcatggatca gcagaagctt
      721 tttctttccg gtgtctttgt atttgtcgat gatggaaaga ccgtagaaga gatgccgtac
      781 ctggatcatc agtgtcatga tcgcggtgga gatcaggctg gcaccgcttg cgagcaggtc
      841 gatcgccaca tactgcatcg agccggcata aataaagatg gacataaaga aagcccagat
      901 aaaagaatat ccgttgcttt caagcagtat gccaaagccc ataccaagaa aaagatagcc
      961 tgccataacc ggaagtgtgt gaggaaaggc agacctgaat gctttgcttt tcatgttcat
     1021 ttcctccttg aaatgtatca gcaattagta tagcatggtg ggtgaaatat gtcaaaatac
     1081 aggagaaaaa agcatgaaat tttatgatgc attaacagat tttattttca cagaacagaa
     1141 accggaaaaa gcagatgcga tctttgttcc gggcggattg tatggtgaga tcgcacagca
     1201 tgcggcagag ctttatcagc agggctttgc atcacttatc gtgccttctg gaaagtacag
     1261 cattgtggaa ggaaaattta ctgaggtaca gtcaccggag aaatataaag gcagatattt
     1321 tgagacagaa agtgattttc tgacgcagat ccttgtggat gaaggggtaa aaaaaagcga
     1381 tattctgcag gaaaaagaag catcttatac ttatcagaat gcattattta ccaaggatct
     1441 tctcaagaca aaagggctac atttaaaaaa agtgatcctg tcctgtcagg catatcacgc
     1501 cagaagatgt ctgatgtatt atcagtttgt tttcccggaa atcgaattta tcgtctcacc
     1561 ggtcattacc aggggtgttt caaaagaaaa ctggtatcag agcgagtacg gtatctgtct
     1621 ggtactcaaa gaggtggaac actgcggcag ccagttcgca gatatgatgt tgcgaccgtg
     1681 cctctgtccg ataaatgtcg gtagagatga caaagattgc atgcaaagtg ttacagaaat
     1741 ttgacggaaa accagtttcg tgttatgctg tagcnnnnnn nnnncagcag aaaaccgttc
     1801 acaaaagagg agtgatctta tgataaaagc agctattttt gatcttgacg gtacgatcgg
     1861 agataccgtg gaatccatcg cttacagtgg caatcgtgcc ctcagagata tcggatacca
     1921 ggcatttccg gtggaaaatt ataaatatta tgcaggtgat ggcgtagatg aactggtccg
     1981 ccgtactctg cgggattccg gtgatacgaa ctgcagtcag tttgagcaga tgaaaagcat
     2041 ttacaaagcg tattttgcaa aagactgcat gtaccatgta aagccgtttc cgggtatttg
     2101 cgagatgctt tcagaactga aaaaaagagg gatccggatc gccgtacttt ccaataaacc
     2161 gcatgctcag gccatcgatg tggtggagag tctgtttgga aaagattatt ttgattacat
     2221 ccagggacag acagaaacga tccgacgcaa acccgcaccg gacggagcgt ttgccattgc
     2281 agaaaagttt gcagtagaga ccggagaatg tctttatgtc ggagatacaa acacggacat
     2341 gcagaccggc aatgcggcac atatgcatcc ggtaggggtt ctgtggggat tccgcgaccg
     2401 aaaagaatta gaagaaaatc acagtgaata tgtgattgaa aaaccacagg aattgattga
     2461 gatcctggaa aagataaacg gaggagacaa caagtgataa aactggtagc aaccgatatt
     2521 gacggcacac tgatacggga gagcagttct gtgctgccgg aagaaatttt tgatctgatc
     2581 cgggaacttc gaaaacgtga tatctatttt gcggcagcaa gcggaagaca gtacatggga
     2641 ctgaaacgac tgtttgctcc ggtggcagat cagatgcttt ttatcagtga aaatggttcc
     2701 aacgttgtat ggaaggacga agaactttat gcttccgtga tcgatccgaa cctgataaaa
     2761 gagatgcagg aatatgcaaa acatttcccg aatatgcatc tgaccttatc gacaacaaaa
     2821 gcgatgcatt ctaccggaag cgatgacctg tcctatcgga aaatgatggt ggatggctat
     2881 cagaatgaca tggtttttga tgatgacgaa gacggcacca aactgaaagt gatcaaaatg
     2941 tctctgtact gtgaaagcgg gattcggcct tatgcaaaag aaatcgaaga ttactggcag
     3001 ggcagattaa acaccgtagt ggcaggtgca ccgtggattg attttatgaa gccaggaacc
     3061 gataaaggtg cagcacttca gttcgtacag cagtattttc aggtaaaaaa agaagagacg
     3121 ctggcattcg gggataatca caacgatctt ggaatgatcc tggcagcagg agagggatgt
     3181 gcagtttcta ccgcaagaac cgcagtcaaa gaggcagcaa aacatatcgt tggaagctac
     3241 gaagaaaaag gggtcataaa ggcacttcag gagctgctaa agaagcagaa ataaataatg
     3301 agatgttgag atcaaaagat atttggatct taatcaatac ttgtaatggg caagtgggcc
     3361 ggagagaaaa aaggaggaat ttataagtaa ctaaatatat tgcttaatat ggttaaatgt
     3421 gttt
//
LOCUS       DAWDWH010000214         3383 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_10187_length_3383_cov_9.629808, whole genome shotgun sequence.
ACCESSION   DAWDWH010000214 DAWDWH010000000
VERSION     DAWDWH010000214.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10187_length_3383_cov_9.629808"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          760 a          995 c          686 g          942 t
ORIGIN      
        1 cactctgtcc gatgaccaca tcgacgctgc cgtctgtgat gctcacgact gcatcggtaa
       61 atcctttgga ttcgagcaga agctctgctg ccgcttcttt ttctgcgttt tctgtgaggg
      121 cggtcatatc gttgactgca ttttgcttct gttcttcggt cacgttttcg ttgttgatga
      181 tctcaagcag ggtttctttg tttttggatc gtacctgctc tctggtcagt tttgcctggg
      241 ccgcaaagct gctgcctgtt cctgtgctgg taagtactgc ctcgcccggc gtgtcggtca
      301 cttcttcgga tgcagtgtcc gttccggcat ctgtttcgcc cgccgacagg gaggcatcgc
      361 tcataccatc ggtttcgcct gccgcagctt ctcctaaata aacttctccg tccgccgagt
      421 cggtaagggt gatgtcctga tccaggctgt cgatatcggt aagtgtggct tcggtctctg
      481 ttgtggatgc cggcagtgca ttttcaccga gtttcgtacc cgaataattt agatagcctg
      541 ccactgcgac taatgccgcc agagctgtga tgataatctg gtttttcttc cacatatgtt
      601 tcacatctct cctccttaaa ccttcttcat tactttgatt ttatgcactt cgattccaaa
      661 taatgctacg accgcatcgg taatatccgc tgctgttttt gcattgtttc cgccctctgc
      721 cgccacgatc acaccttcca cttccggctc tgtaatttct ttgacatacg gcgtttccgc
      781 tccgttttcc tgcttttcaa aaaccgtgtt ctcttcgaaa tcctgtgcat ccgccgtgct
      841 tttgtcgccg gactgatcct gtgtattttg actgtttgag gtccttgtgt ccttttccac
      901 aatatacgct ccgcaggaac gtaaggtaat catcactttg acttccccca ctccgtccac
      961 ttcccgaagc acttcttcaa gcctttgttc catcctctgt gtccgttcct gtgccagatc
     1021 ttctgtattt cttacgttgt tttcaactgc cggtgcattg gtttttgcct gtgtgttctg
     1081 ctttttgctg ctgttatccg gccaggtgat caccagaagc aaaagtccga caagaagacc
     1141 gatcagcacc ggactgccct tttttccttt gcatttctcc caaaaatcca gccactgttt
     1201 catgcggctc atccccccaa aaagaccact ttatcgctgc tgatctgata aatttcccaa
     1261 agtttctgta aaatctgctc ctgcaccgcc tctttttgct gcagatcctc ataccccccg
     1321 gaaatcgtta ctttgcggat gtcctctgtc tcttcggatt gaaatacaac cttcgctttt
     1381 gtccctttca tcccctcccc gtctgccgtc tcttcaatct gacgctgcac ttccttctga
     1441 caggcttccg tcagaacacg gctccttaaa tcacttaaat tttcctgttc catccggatc
     1501 gaatcccact cttccagctg taatgcctgc tgccactgtt tctcaaatac tgccgtcaga
     1561 tgtgcctgat ccgtcagcgg actaatcagc aggatcagaa acaggacacc gcaaaacagc
     1621 cgaatgtatt tctgataatg gttatctgga agaacctgct ctgcaagctg catcagacac
     1681 aagcagcaca ggatgctccg tacccagctt cgaatctctg ccgacatctt ttctcccctt
     1741 tcttctattt tccaagcgat gcggtgacaa tggcgatcgt caggaaaaac agcatactgc
     1801 tggacaccat gatcttcaga agcagcccga ctccttctgc gactgccgcc acacattcgt
     1861 tgagccgttt gtcaccgacc ggctgcacca gagccgccat cgccttatag atcagtgtgc
     1921 agaacgccag acggcacacc ggtgcgagac agagcatcag aacagcgatc atcccgcaca
     1981 caccgacggc attcttgcca atgccgggga ttgccgatac ggtgcggctg acggctgtat
     2041 ttttcagccc gtcaacggcc ggaaggatca gtccctggat cacctgaaat ccgatcgctg
     2101 ccgctgtcat gctcttcatt gcccattcga tcaccgtacg cacaagttct gccagatgcg
     2161 agagcctgtc atcctttgaa agatggtcga gcagcaaaaa caggacataa agctgcacac
     2221 cgggcagaag aagatatctt tgcacccact gcaccgctgc gatcagcagg atcgccgact
     2281 cgtaaaacgc cagccctcca acgctgttcc ccgcaaaaac ggccgccgca aaataagacg
     2341 gcaccagagc tttcataaag ccattgactc tgccaagcgt tgcatccgtc atccggctca
     2401 ttccggtaaa tcccttcatc agaactgctg ccagcatcag atacatcata aaaaagctga
     2461 tcagtgctgt accgttcttt tccatgagat ccgtgaaatt caaaacaagt gccgccacga
     2521 tcaccagtaa aaacacctgc agtgccgtct ctttctgatt tttccattcc agaagcagtg
     2581 catcaaaaac cagcgttttc aaagttgaaa gattcagcgg gatttctccc ctgatcaggc
     2641 ttttcagtgc atccgcaaaa cgaaaactgc tctctttctc cagatctgca aggctctctt
     2701 cgacttcctg taaatgaaac gcctttatat taaggaagtc ttccgctccg gtctgtaatt
     2761 cttctgctgt atcctgtccc tgatctttct cctgcacctg tgatgttgcc atgatcgccg
     2821 ttttttctga aagaacggcc gcctcgcaca cctgcttctg tcctgccaca tccaaccatc
     2881 ctgcaaaaag cagcaaaaac acataaatac gatatttctt catatatttt accatcaggc
     2941 aagacagctg cggatcgtct caaacagtgc cagcaaaacc ggtgtccctg tcgccagcac
     3001 cgagatcttt ccgaaaaatt caatctgtga ggagattgcc ccatatcccg catctttgca
     3061 gatgcctgcc gcaaaatccg ccacataggt gataccgatc attttgaaca gaatcttcac
     3121 ataagcatcc tttatgtttg tgtagctttg cagctgctgc aggctttctg ataaatacct
     3181 cagtttttcc actgcacaga aaaaaataag catacaggaa gccatgctga tcaacacggc
     3241 aaacacaggt ctggtctctt tgagaaacaa cgccagtaaa atcccggtaa tcccaagaac
     3301 tgcaattttg atcacagtgc aaacaactcc tttatgctct caaacagctg gctgatatac
     3361 ggcagcaccc agaacagcac cag
//
LOCUS       DAWDWH010000215         3382 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_10193_length_3382_cov_9.649534, whole genome shotgun sequence.
ACCESSION   DAWDWH010000215 DAWDWH010000000
VERSION     DAWDWH010000215.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10193_length_3382_cov_9.649534"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2229..2238
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          995 a          621 c          850 g          906 t
ORIGIN      
        1 catggtcttt gttaagatct tatcgcttgg ataacgtctg gtggaagcca gatgagaatc
       61 catgaaggtg gttacctgat ccatatataa ctgtatatcg ggaatatcct cggacttcac
      121 gtaatcaata cggtcaagac tgtcgagaat actctccaga agctggtctg tgttgattgt
      181 cattgcagta tcctccaaaa tccgccctgc gggcttcttt ttcatgtttt tgcataaaag
      241 tagtacaaga taataccaac ggattgctcc gtgcgtcgca cttccacaat cctttctacg
      301 ctccatatcg ggcggagcca aagatctttc atccacttat gagcaagctc atgtggagtt
      361 agaccttttg atcctggcat tatcgtatta cattatatag tttttaaaac cagatgacaa
      421 gggctttgat aaaaaatatc aaaagataat ctgatgaatt atttaatttt tcttaaaata
      481 aaataattgg gttgacattt tgcattcatg ctggcactat atctgttatg agaaaacacc
      541 cgttttagaa tacttgtaag aaagaatttg aaatgtgaaa agggaggtaa tcattataaa
      601 agagatcata gccaggaaga aaacactgct ttgcagagtg gcggtgattt taggcttttg
      661 tattttttat ctgtcaggtt tttcatggct ggagaacagg catgtttccg gcatacatat
      721 tactgcctgt gctttggatt ataagattcc gtttcaggag atttttatca taccatattt
      781 attatggttt gtttatattg cggcgacggt gatttatttt ctgttgcacg aggatgagaa
      841 ttttaatgca cttgctggga cactggctct tggtatgaca ctttttctgg ttgtatctgc
      901 catttatcca aatggactga acctgcgtcc ggtgacgttt gaaagggaca atatttttat
      961 caggctggtg cagtttgttt ataagaacga tacaccgacc aatgtactgc caagtattca
     1021 tgtatacaat tcacttgctg cctttatggc aatcaaaaga agcaagcttg cccggaaaca
     1081 aaaaggctgg gcatggggca ctggtattct gacggttctg attattttag ctactgtatt
     1141 tttgaaacaa cattcgatgt ttgatggagt ctgtgctctg gcactgtacg ttgttacaga
     1201 taaactctgc tatggtgtga atccggaaac agagcggagc aaagtacgaa atcagttaat
     1261 ttaggtggag gaaattatga gtgtatatga cacactgaac gataaacaaa aagaagcggt
     1321 ctttcatacg gaaggaccgc ttcttgtact ggcgggagcc ggttcgggaa agacacgcgt
     1381 gctgacacat cgcattgcct atcttatgga agaaaagaag gtaaagccat ggaatattct
     1441 ggcgatcacg tttaccaata aggcagccgg tgagatgcgt gagcgtgtgg ataagttagt
     1501 cggagatgcc gctggaaagg tttgggtttc tacctttcat tcagcatgcg tccggatttt
     1561 gcgacgggac attggatgga tcggatttga caccaatttt gcgatctatg acaccgaaga
     1621 tcagaaaagc ctgatgaaaa gtgtctgcaa acagcttcag gtggatacca agctttataa
     1681 agaacgggca ctgatgtccg cgatctcggc agcgaaagat gaactggtaa gtccgcagga
     1741 gtttgcactt cgggcacagg gaaatttccg tcaggagacg gtcgcaaggg tttatgcaga
     1801 atatcagaga cagttaaaaa gcagcaatgc tctggatttt gatgatctga tcatgaagac
     1861 tgtggaactg ttccaggcaa attccgatat tctgaatgaa tatcaggagc gtttccgtta
     1921 tattatggtg gatgagtatc aggataccaa tacggcacag tttaagctgg tcagccttct
     1981 ggcagcaaaa taccgtaatc tctgtgtggt tggtgatgat gatcagtcga tctataaatt
     2041 ccgcggagca aatatccaga atattctcgg atttgaaaaa acatttccgg atgcgacagt
     2101 gatcaagctg gagcagaatt accgttcgac acagaacatt ttaaatgcgg caaatacagt
     2161 gatcgcccac aataaaggac gaaaagaaaa gaaactgtgg acggcaaacg gggaaggcga
     2221 taaagtccnn nnnnnnnnaa agtccgggta cgcagtttta tgtctgcgta tgatgaggca
     2281 gagatcattg tcggagagat tgctgcaaaa gtacgaaatc aggatgccca gtacggagat
     2341 tttgcggttc tttatcgtac caatgctcag tcacgtatat ttgaagaaaa gtttctgatg
     2401 gcgaatatac cgtataagat cattggaggt gtaaacttct atgccagaaa agagatcaaa
     2461 gatctgctgg catatttaaa aaccattgat aatgcaaggg atgatatcgc cgtaagacgt
     2521 atcttaaatg taccaaagcg tgggatcggt gcgacgacga tcaaccgtgt gcaggaatat
     2581 gcaacggaac agaatgtcag cttttatgag gcactttctg aaatttcaaa gattccgggc
     2641 ggcggaaggg cagctgccaa aattgagagt tttgtgacgt tgatccgcac gctgcggaca
     2701 caggaagatt atttcagtgt acaggagctt ttgcaggaag tgatcgaccg gaccggttat
     2761 gtaaaagaac tggaagcaga aggcacagaa gaagcaaggg cacgtattga aaatatcgat
     2821 gaactgatca acaaagcagc ttcctatgaa gaagatgcac aagagccatc actggaaggt
     2881 tttctttcgg aagtggcact ggttgccgat atcgacagta tggatgacga tgccggaaga
     2941 gtgattctga tgacgctgca cagtgccaag ggactggaat ttccgcaggt ttatctgacc
     3001 ggtatggaag atgggctttt tccaagttat atgtcggtgg attcggagga tccggatgct
     3061 ctggaagagg agcggagact ttgctatgtc ggcatcacaa gagcgatgca ggatcttact
     3121 ctgacacatg cgaaaaagcg tatgacgcac ggagaaacac attacaaccg tccgtccagg
     3181 tttctaaacg aacttccgga agagctgttg aaaaaagaga aacgggaaga gccaaaggag
     3241 caggagccga agccgcgtgt tcagacctct ttcatgcagg caaagagcat ttttcagaca
     3301 aaggcttatg acagtgtgtt tgatctgaaa aaaggaatgc cggcaaaagc aggcagtctg
     3361 gactatgccg ttggagatac cg
//
LOCUS       DAWDWH010000216         3379 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_10203_length_3379_cov_11.558363, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000216 DAWDWH010000000
VERSION     DAWDWH010000216.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10203_length_3379_cov_11.558363"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    976..985
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          881 a          806 c          700 g          982 t
ORIGIN      
        1 aaacagcggt accacaacaa ggctgatctc aggtattctc gccggacagg atttcacatc
       61 cgaactgacc ggagatgcat cgatccagaa acgtccgatg ggacgcatca tcagaccgtt
      121 atccatgatg gatgccacga ttgaaagtat caaagaaaac ggatgtgcac cgcttcgcat
      181 cagcggacac gctttaaagg ggatccatta ccagtcaccg gtcgcatctg cccaggtaaa
      241 atccagcatt ctgctggcag gactttatgc tgacggcaaa acttctgtga ccgaaccggc
      301 attatccaga aaccatacgg agatcatgct tcgttctttc ggagccacgg tcacttctga
      361 ggggactacc gcatcgattc tgccggatcc ggatcttcat ggacagaaaa tagaagtgcc
      421 tggcgatatc tcttctgctg cgtattttat tgtggctgca ctgattgttc cggaatcgga
      481 aatcctgatc aaaaactgcg gcatcaacga gacaagagat ggcatcttac gtgtctgccg
      541 tgcaatggga gcggatatca ccgtcttgaa tgcacataca tccggcggtg aggcagttgc
      601 agaccttctg gtacgctaca cgccggatct gaaaggctgt gtgattgaag gagatatcat
      661 tccgacgctg atcgatgaaa taccggtcat tgctgtttta gctgcatttg cagatggaac
      721 aactacgatc cgaaatgctg aagagttaaa agtaaaagaa tccaaccgcc tggatatcat
      781 ggtgcatcat ttaagtgaaa tgggatgtga tatcacagga accgaagatg gcatgatcat
      841 aaatggaggc aaaacgcttc aaggagccgt tcttgacagt tttctggatc accgcatcgc
      901 catgtccttt gcgattgctt ctctggcatg cgaaagcact ctgacgatcc ggcgtgccga
      961 ctgtgtcaac atcagnnnnn nnnnnttttc ttttttcaag gaaacaggtt ttgcttcttt
     1021 atttaagaag caatgcctga tttttccggt tgtacataat ttcccatctt tctgatgata
     1081 aatttcatag caaaattcca gaacgatccc attatacttt gcagctgtca ctttgatatc
     1141 cacaagatca tcaaaatgga ccatctgacg ataagttgct tcaactccaa gcaccggaac
     1201 gatgatgccc tgtttttcca tttcacggta cgaaatcccg cagtgccgca tccagtcgat
     1261 ccgtgcttct tcaaaccagc ggatatagtt ggaatgatgt acgatgccca tctggtccgt
     1321 ttcataatac tgtactctgt gtgtatacat acaaaatcca ccccttatca gattagccca
     1381 taatgtttca tggcaaatgc aatgccgtcc tcttctaccg ttttcgttac aaattccgca
     1441 tacggttcca gaacattact gtgtacgccc atggcaacgg catgttttac atactgaaac
     1501 attggaagat cgttgctgct gtccccgaac acataggcgt tttccattgg aatgttcatg
     1561 tgttccagaa tataggcgat gccgctggcc tttgaaaagc cttttggaac aacttcgaaa
     1621 aatccgttct ggcggtcgat cagatccata tcttttccaa gatttgcctt gatcgccgca
     1681 agattggtct tctgatcata ataaaacaca aatttatcat aatcaaaacg atcctgttct
     1741 atgtaggttt cgattccaag acccatcggt gcgaaataac gcctggtgtg ttcgatccct
     1801 tcaaatctgg ttatccgctt tgaaaaatag cagtcgtccg ttgcttcgag gatcggttct
     1861 gcatgattct cctgaagcac acggataatc tctcttcctc ttttatgcgg tataatacgg
     1921 gagaacatca cttcatcgcc aagcatgatg taggtgccgc atccacacag atatccggaa
     1981 aacggtgcac gttttaattc catcggaacg ctgcagtagc atctgccggt attgataaag
     2041 gtcagatttc caagttcaat cgtttttttt agagcatcga gtgcactgtc cggtatcttt
     2101 ccggtcacat cacttaaaag cgtgccgtcg atatcaaaaa ataatgcatt tctcatgttt
     2161 cgtatccttc ctgaaataaa gaattggtat gctgtctttt cggcagcatt atactatgca
     2221 tcaaaagatg ctgcaaagtt tttcttatac ttttgcatca aaaaaagctg ccggctgact
     2281 ttttgctgtc agccagacag cgaacttttc attcccagaa tgccgtttcc tgccttttga
     2341 ttcctggcca ggccaggtgg gtatccgcag ccgtatccgt tgtcttccat ttacaaaaaa
     2401 cggcatgaca ttgtataaag caatatagga ttccctgtta taacattgca ggttcaaaat
     2461 aacaggagtg tcgcacatcc caggtggatt tctcactgac tttattatac tccagtatat
     2521 ccgatttttc aactgaaaat tcaagcaatt ttagtttcac tgttcccgca tgatgatact
     2581 gatcacataa cggttgttcg gaagatccat atgtgtgata ttatccagac gaatcagatc
     2641 aacatctacc cacagctgtt ctgctgcaat cataatgttt gcgagcatga tgccgaaatc
     2701 aaattcgttg tatttgccaa gcagtctgcg atgagcgacg gtctgtttgg aaaagacatg
     2761 gacacggttt tcataaacga caaaacgcca tggctgactg ttaaatgcag acggtgccag
     2821 tctggccact tccagaaatt cttttacaaa tcttctcgga ggttctttga cggcacagag
     2881 atcttccatg gattcccttt tggcatcttc ctgattccgc atcatatcta ctttcggata
     2941 tccaattgcc atcaggatga cgcatcgcat gccctctttt ggcatttcgc ctttcagatg
     3001 tcccataccc tggaagcagc ttccaacacc ttttgaaaat agataaagcg agatctgctg
     3061 catgatatag ccggcattca tatccgctct ttctttgtcc tctgtataga tcacaagata
     3121 atagggagct actacattga taataccgcc cagcctggac tttgcttttt tactgccctg
     3181 tgtatcgatc agttcgatgc tggtctcgat ccccgggaac agcggttcca tttcatggta
     3241 aaactggatc atcccttcca gaatacttgt ctctacttta tccttgcgaa aatttctgat
     3301 tgatttgcgt gtgtacactg cttcgtatag cgtcattttg tctctcctgc actttcagca
     3361 tcttgttaat aacttgtaa
//
LOCUS       DAWDWH010000217         3363 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_10249_length_3363_cov_12.639359, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000217 DAWDWH010000000
VERSION     DAWDWH010000217.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10249_length_3363_cov_12.639359"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          901 a          705 c          763 g          994 t
ORIGIN      
        1 tattttccat agctcacatt tttccctttt ttccggaaag ccctcacgcg atagtacacg
       61 gtttttcggg agcgtttctt tattacaaag cttgtttttt taccaacctt tttcactttt
      121 acccatcttt tattctggta acggtaaatc tcatacccgg aggcattttt tacttttttc
      181 cagccaagga ttgccttgcc ggatttttta taagaaatct tcactccggt tacttttgca
      241 ggtgcggtcg gtttcgcagc cggttttggc gtttccgtat tgccggactg atctggtacg
      301 tgtgtttccg gtgtttctgt atccggctgt ggcttttcgg tttcattggt atccggaaca
      361 gtcggtcgaa cttccagttt tttgattgca gtttccagat tttttgccat ttcgtcaacg
      421 agattctgct gtgcggcagt gcagtcacgg ttgatggcct ggatggcttc ttttaagcag
      481 ctgacgctgt ccggtgtgta ccggtaaaga tcagaatcat ccggaacggt tttcagcagt
      541 tcatcgatct tgctgtaatc tgccgggaag agttcttttg gatctttgca ggtcagtcct
      601 ttctttttgg cataagcttc tcccgcactt ccggcagatg catatacgac aaagttattc
      661 gggcatccgt tgaaagcttc gtaaacattg ccattgtttt cttcatatgc gatcgttgtc
      721 acactgtccg gaatataaac tttttccagg tgctcacagt tttcaatggc atcttcttcc
      781 agttcttttg taccttctcc gattttcaga atacgcaggt tcgaaagatt agtgagcgta
      841 tttttttcca ggatctccat cggtttcaga gtaacttcgg aaatacctgc cgggacagag
      901 tagattcttg agtaatcttt ggaataaagt attccgtcat aggacgcaaa taccggattg
      961 ttttcatcta cggaaataga ttccagagat tttgaataga aagaacggat ctcatagtca
     1021 cttaagtaga ttccgtattt tactgttgcc ggaagcacca gttcttttgc tgctgtatcc
     1081 tgaaaggaat gttcataata aatctggctt tctgcggcga tactgatcac aggataacca
     1141 ttgcagcttt ccgggatttc cagacgctcc gcatttccgg tatagccgac agcaccaatg
     1201 ccgcccagat agtaagtaaa ttcaaagccg tccttttcat aaagaacagc ttccaggctg
     1261 tctgttttat aatgagaaga gatgtcggaa ggatcatcca gtgagatctg ccacatggat
     1321 gcatcgacga tgccccattt gccgtcacac agaacgaaat cccatgcatg gccttcggtt
     1381 tcccagtagc cgtgggcgat gatacatgga acatcgatgg cggcgagcat ggtcttacag
     1441 agattggctt ttccctgaca tactccggtt ttctctttga atactttata aggatcattt
     1501 tcttccagat ctgcaggggc agattcgccg acagaagctt tcaggctggt gtcttttgcc
     1561 cactgataga tggcagtgat cttgtctttt gtggaactgt atttttctgt taaatctaaa
     1621 gtaaatgtac ggattgtctg atactgtgaa tccgtgatat tcagatgctg tgttccgata
     1681 aattcttgtg cggaattgct gttaaaatca gaagtgagaa tctgaagaca atcgtcggtg
     1741 gtgtttgccg catttgcggt tgtatgcaaa ggtatgacca ggcatacaaa cagaaaaaaa
     1801 tgaagcaaaa cagaatacat attttttttc atgaaataac cctctctttc taaaaagata
     1861 tcgtgtttta cggatgaaaa gaatatggta atcttttcgt aactgtgaag gtagtgaata
     1921 gttacatctt ttcattgtaa tccggatgtt atattacata atcatttgca taagtttcac
     1981 gctgctgatc cagaagatcc tgcaaagtga tcccatctac atagtcatca atgacttttt
     2041 tcaatccttt ccatacggaa agtgtcgtgc acatgtcggc tctctgacag agtaccggct
     2101 cattgtccag acaggcaacc ggtgacagac ttccctccgt tgcacgtaag atatcaccga
     2161 cggtgtattt gtccggtgtt tttgctagac ggtatccgcc ctgaaatccg cggcttgcga
     2221 gcaggaaacc ggatttgttc aggatcggga tgatctgttc cagatatttt ttggaaacat
     2281 cctgtcttgc tgcgatgtct tttaaagcga tgtaaccctc gctctgatgt tctgcaagat
     2341 ccagaagcat acgcagagca tatcttcctt ttgtagaaat tttcattttt tctcacctcg
     2401 tttcatatat tagcagtgtt gaaagtgttg tcctccggca tgacgcctgt gaattgcttc
     2461 actgccgggt gatttcagca atggaacgtt caaccttaga atgatagtat catatttcct
     2521 atcgtatttc tagagattta aaaagggaag tctgaaaaaa atgaaaaaag gtgttgacaa
     2581 atactggatg ttggcatata attcgaataa atcatataaa acatatcgaa aaggtaggaa
     2641 taaataagga ggaaaataac atgtccaaca tttataaagg aactttagga ttgatcggaa
     2701 acacaccact ggtagaagtt gtaaacatag aaaaagatca ggagctttca gcaactgtcc
     2761 tggcaaaact tgagtacttt aacccggcag gaagcgtaaa agaccgtatt gcaaagagca
     2821 tgatcgaaga cgctgagagc aaaggacttc tgaaagaagg ttctgttatc atcgagccga
     2881 catccggaaa taccggtatc ggactggcat ccattgcagc agcaaaagga taccacatca
     2941 tcttgaccat gccggatacg atgagtattg aaagaagaaa catcctgaaa gcatacggtg
     3001 cagaactggt gctgacagaa ggtgcaaagg gtatgaaagg tgccatcgca aaagcagatg
     3061 agctggcaaa agaaatcccg aacagctaca ttccgggaca gtttgtaaac ccggcaaacc
     3121 cggcagcaca cagagcaacc acaggtccgg aaatctggaa agacacagac ggcaaggtag
     3181 atttcttcgt agccggtgtt ggtacaggcg gaacactgac aggtgtcggt gaatatctga
     3241 aagagcagaa tccggatatc aagatcgtgg cagtagagcc ggcaacttcc ccggttcttt
     3301 ccgaaggcaa gggaggtcca cataaaatcc agggtatcgg tgcaggattt gtaccggaag
     3361 tac
//
LOCUS       DAWDWH010000218         3349 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_10300_length_3349_cov_12.804189, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000218 DAWDWH010000000
VERSION     DAWDWH010000218.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10300_length_3349_cov_12.804189"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          734 a          824 c          636 g         1155 t
ORIGIN      
        1 tactttgaat gcctgcgagc caatcttaac tgcatttttg ttcttgaagg tgatactctt
       61 cagtttgctg tttttataga agctctttgt tccgatcttg ctgatatttg ttccgagaat
      121 cagcgtcgtc aattttttat tgttctggaa tgcttttgct gcaatttccg ttactttaaa
      181 ggtatagcca tctttcgtaa cggttgccgg aatggtgatc ttatttcttt ttttcctggt
      241 tccggttact gcaaccgttc ctttttttgc atcggatttg gtcactttat agctgattcc
      301 ttttccgtcg gtaaataccg ttcccttttt cggtaccgtt ttgacagtat ccggtgccgg
      361 atttggattt ggtgtcggcg ttggcgtcgg atctggtttt tgctgttctg ctttttccgt
      421 cagagctttt cttgcctctt ccagttcttt ctttgctgcg tccaactctt ccggttgtgc
      481 atctgctcga tccagcatct ctttcgcccg tttcagtgct tcctgatatt tggctgcact
      541 ttcagaagtg tattttgaaa gatcgaattt ttcagacacc tttatctctt ccgtcagagt
      601 ttctttgcat cctgtaactt ttttcaggta agcattctgt gcactttcca gctcagcttt
      661 tgccgcatcc agttcttcct gaccagccat gacatcaccg tataccgctt ttgctctcac
      721 agacgcattc cataattctg ccagttcatc tgtgtttgca gatgcataag caaaatcagc
      781 ttttgtattt ttcaagatcg tttttgcttc ctcatatgca tttttcaggg cacttctgtc
      841 aacgctctgg tatactttca tttcacaaat tgcatattca cttgcttttc gctgtacacc
      901 cttcattctg acatagcgtg cggatgtttc tgtaaagctg tcttcagtcc atgctttttc
      961 attgccgctg acggatttta ctgtgctcca gtcggcacca tcgtttgata cctgaatgtc
     1021 gtatgctgtc gcataacgtc ctgctgccca gtcgatcaca accttggata cctttctttc
     1081 tcttccaagg tcaatcatca ggaaatcatc atttcggtca gaagagcacc atctggattc
     1141 gtaagaggta cttactccgc ttgttccggt tacatgtccg tctccgtcgg ttgccttcca
     1201 tgccggatag agacgcatct catccccttt gtagcctttg ttgccaagat cggtatatgc
     1261 tccccggttt ctcgccacat ccacaagcgt tgcaccaaca ttttctgctg ccagttcttc
     1321 tgtggacagt gctttgttat atgctttgat atttccaagg taaccggaga aattctttcc
     1381 gatctcattt aacgggaaca caaaggtgct gcggtaattg ttgtctgcat ctgtccaggt
     1441 acttgcaccg agtgcaccat tgttctccgg atcgctggct gccgcataaa gaatcttttt
     1501 aaacacacca tctacataca gttttgttgt ctgatacgtt cctacgaccg tgatccggtg
     1561 ctttttattc ttttctacct gatactcaaa ggactgatta aaataatcgc ggttcaggct
     1621 cagggaacca ttgatacccg caagctgcag tcttccgtca taaccggaaa acagagagga
     1681 ttctttcgta ttcttttctg taccgtccag atatacatca aaactcatcg tatatggata
     1741 accaagtgtc gtcagtggtg tacggataac tgtattgcca tcaaatttca gataggtttc
     1801 gccgtctttt tctgcttttt ctccaccgct cacctgtccg ttgtaagcat ttccgctggc
     1861 atcataaatg atttctccat ccgcggataa gtttgcaaaa tcatagtcca gcactacatc
     1921 tgaaacactt tccatatcat aggaaatagc agttccaggt ccctcgcgta atgcatcaaa
     1981 ggtctgctca tagtttacaa agcttgtttc tttattgctg ccccaggttt tctgtgatac
     2041 catggctacc gcacgcagat aacgctcatt cagatcggct tctgtgatac cctcgcggtt
     2101 ctcatcgccc cagagtgctg tcttggctcc aagtacctgc ggatcacctt tatccgtctt
     2161 cactccgccg ctgaacacag acggatccca gttatagaaa agattatttt cctggatcat
     2221 atctttgtga taacgtccag gtgtattata aagatatgtc tgcggtacat tgataagacg
     2281 gaagccttct gccagtcttg cactcggact gtcctctgta gcagaccaca tattcaggat
     2341 aatattatta tttacctttg tatttccctt taagacttcc atcgctcccc acatttccac
     2401 ttcgacatca tacttatcat gcagatagct gtacatctca ttcatgtaac ggcggaaagc
     2461 ttctttattt gcatcggttc tgtcccagta ttcatcaaca cctgcgttaa cattttttgc
     2521 atcaaaaacc gcatcaattc cgtcaattcc tccgagatat tcatcaaaca gtgctttcat
     2581 aaagattctt gcattgatcg cattctgtct gatctgtgca ttgctgctct gatcgtccag
     2641 tgccaggagt tcccagtttc cagggttgtg aatgtagaaa gaagctccct gttttacatt
     2701 tccgtctgcg ttcagataat ctgccgcctt cagatagccg tattttacaa gtgatttgat
     2761 cacttcttcc tgatggtcat gaacatattt ggtgtaagct gcggaatgtc ccggtgtatc
     2821 taattccgct acaacctgaa taccgcatgc ctttgcttct tcgttcatct tgcggaattc
     2881 ttcttttgta tagtaaagct caccgctttt accggtatgt acattataat agtagtcata
     2941 gcgatcctca ctgtctcctg tttttacaaa tttggacttt tgtgttgcaa gagacggaaa
     3001 aagttctgat tccagtctgt gagaagcctc ggtatctgcc caatcctcat attttgcaga
     3061 atacgccgga tctgaccact gattatcatt gatatgaagc tgcatctcat tcagtttgta
     3121 ccatttcatg atttttgtat aatcattcag gaatgatatt cttgtaggga tacgggcaat
     3181 gtcaaacatc acgcctcgca tctcatatgc cggataatca cgaatcacac cttttggcac
     3241 atttgcatgt tcactgtcct gataaaggat ctgttctacg gtcacagtac cgtaaagaag
     3301 tcctgttttg gtggaagaat aaattttcag tccagcatcg ccattaacc
//
LOCUS       DAWDWH010000219         3348 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_10302_length_3348_cov_9.606742, whole genome shotgun sequence.
ACCESSION   DAWDWH010000219 DAWDWH010000000
VERSION     DAWDWH010000219.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10302_length_3348_cov_9.606742"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1403..1412
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2346..2355
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          787 a          865 c          644 g         1032 t
ORIGIN      
        1 tccgccttgc tggactctac aaactcacct cgctccttgg tcgggttggt gtcatcaaaa
       61 cgcaagttaa acttgccgtg atatttctgt gcaagtccat aatttaacag aatggctttg
      121 gcatgtccga tatgcagata gccatttggt tccggtggaa aacgagtaca gatcgtatcg
      181 tatttgccct ctgccagatc tttttctata atctgttcaa taaaattttt ggaaacagtt
      241 tcctctccca tgttgtcctc ctaaaatatc cattttttca actgtgaaaa ttctaccata
      301 aatctcacag aattacaacg tcctatctct gccgcttttg tatcagttga atccataaaa
      361 tttctgtgag atcttatagg ctgcaaccga aagttttctt cctcctctgc ccggcaaatt
      421 catcgtacgt ccaaacagac cgtgacgcag gcggaaatac agaccgccgt cttttgcttt
      481 taaatactgc cacagttctt ttttcttttc cagattttct tctgtgccgg aacggatcag
      541 cataacggaa gtaaccgttg taataatatc cagataactt gtcatatact ggcggattcg
      601 cttgtctgcg attttattgt cagccatggt gtcgatcatg atcttattga cacggatctg
      661 ctggtcgatg cgggagatca tgactttttc gttgacggac tgatcatccc tgccgataaa
      721 ataatgatag aaattcacat ttttataata aagatttttt acatacggaa gcggctggaa
      781 cacaaacaga ttatccacgt aaaaggtatg tttcggaagc tccaggttgc attcccgtag
      841 cagcatggta cggtagatca cggaatgcat cagaatgtaa cgtccgatgt gcagtttttt
      901 tgcatgatcc cactggaata cttcattttc aggaaatgcc ttgtaccgca tcactttttt
      961 gtgcttcgca cctgccttgt cgtagacaaa attgctgatg aacatatcaa cagtctgcgg
     1021 acctgccacc agttctttca aagttttgat aatatcgaga taaacatcca catctaccca
     1081 gtcatcgctg tccaccactt tatagtatac tccggttgcg ttacgcagtc cggcattgac
     1141 tgcctctcca tgtccgccgt tctcctgatg gatcgccttt acgatccccg gataattttt
     1201 ttcatactca tctgcaatct ctgcggtatt gtcttttgtg gaaccgtcat ccacaattaa
     1261 aatctccaca tcttctccgc ccgaaagcag agaatcaata catcttctca tataggctgc
     1321 agaattataa cagggaattg ctattgataa tattttcacg ttttttcctc catacacgtt
     1381 tctgttttat ttttcgtcat atnnnnnnnn nnatttttcc tgtccgagtc tgatctgaag
     1441 ataggctgtg taagctgaaa aagctgccgg gatcgggtat tcttcttctg ttcttttcgg
     1501 ctcaataaaa agcatatctt ttccagaata gttttccagt tcttccacca ggaccacata
     1561 tcctgtcata tgccattcaa tatgactgaa aatatgtttc gcatccgata atctccggat
     1621 acggatcggt gacagccctt cctgtttcag ccagtccaga acttcatcct ggcttaacca
     1681 tccagagaga ttaggaagct catataaacc tgccagaagt ccttttgccg gacgtttgcg
     1741 gattgctgcc ctgctgccgt cccttatcac cagaacggtc tttttttcga ttttacgcgg
     1801 ctttttctgt gcttttttcg gatagtccat ctctttgccg ttctgatgtg caaggcatac
     1861 gcctgacagc ggacattcct cacacttcgg tgccccgttt ggcacacata cagtcgcccc
     1921 aagttccatc agtgcctggt taaaggctcc ggatcttcct tttggcatta ctgccagaag
     1981 ctcctcctcg acctgacgtt tgacagactg cttcaaaata tctccgtcat tctgcgtgat
     2041 ccttgtgatc acccgcagga catttccgtc aacagccgga accgggatct caaaggcaat
     2101 ggaagctact gctcctgccg tataaggacc gatcccggga agacccagca gtttttcata
     2161 gtctgccggc atttcgccgt cataaaattc caccatattc tgtgctgctt tctgcatatt
     2221 ccggacgcgg ttataatagc caagcccctc ccataacttc aaaagcttat cttccggaca
     2281 ttccgcaagg gatcgaacgt ccggcagagc ctgcgtaaaa cgttcaaaaa agggttttac
     2341 cgcctnnnnn nnnnnggctg ttctctccac gggagaatcc gtgcatgttc cccgaaccac
     2401 ccaagcagtg gctctacaat ctgttcgagc atacatttcc tctttctttt ttcatttaag
     2461 aatgcctctg tcattcttgc tgcgaggtgc acatcatgca tcgcatgaca tatgaatcat
     2521 ctcacatcta atatttatac tcaccgttcc gatagaagca aatgaccaga tagtcttttt
     2581 cgatattctg gtaaatcgtc tgtgcggcac tgtacggaag attaatgcag ccatgagaac
     2641 cgctgttcca gaagatctga ccgccgaagc ttcctctcca ggtggcatcg tggaatccga
     2701 tcccgccgtt aaacggcatc cagaaagaaa ccggtgtctc gtaactgtcg cccggttttg
     2761 tgcttcgaag aacagccggt gattttttat agtataaccg gaaaatgccg ggcggtgtca
     2821 cacgagccgg tgtcattgtt ccggatacga aatccgattc tacgatcaat ttcccttctt
     2881 tgtacatcca cagatgttgt tttgtcagat caacttccac atacgtgtca cccatatcac
     2941 cattttccca gctgtctgcc tcctgggcaa agatcggtgt acgttccatg tgcggctctt
     3001 cttccatcag ctttgtcagc tcttctgcct ccgctgtctg gtcgattttc catccatatg
     3061 aaccgccctc cacagtcaca tacgttccgt cacttgtctt gaacttccgt ggcttgtcat
     3121 aggtatcata cttgtctgcc agagatgcga tatagtcgct gatcgcaccg tcttttacca
     3181 gcactttccc ttttttatca taggtcagcc agtcttttac cacactgccg tccagaacct
     3241 ccttctggtt tccaaaacag tatgtaatct cgatatccac ataatgattg agattctcta
     3301 ccagctggtt taagtctttg tcatcacttg tcacttttgg tgtctgat
//
LOCUS       DAWDWH010000220         3299 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_10461_length_3299_cov_8.906905, whole genome shotgun sequence.
ACCESSION   DAWDWH010000220 DAWDWH010000000
VERSION     DAWDWH010000220.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10461_length_3299_cov_8.906905"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2520..2529
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          860 a          745 c          653 g         1031 t
ORIGIN      
        1 tgcactttcc ggaatcttgt agtttgcaag gcagtcatcg acagtcttca ctacagaatt
       61 cagcttgctt aacagccatt tgtccataac aggaagattg tcataatcta aagtatattt
      121 tgtcgggtca aaattgtcaa tatttgcata aagaacaaag aatgcgtagg tattccacag
      181 agtacccatg aacttacgct gtccttcctg aacagctttg ccgtggaagc ggttcggcag
      241 ccatggagca ctgttgatgt agaagtacca gcggatcgcg tctgcaccat atttttcaag
      301 tgcatcgaac ggatcaactg catttccttt ggacttactc atcttctgac cattttcatc
      361 ctgtacatgt ccgagaacga taacgttctt atatggagcc tggttgaaca ggattgtgga
      421 ctcagcaagc agggagtaga accatcctct cgtctggtca acagcctcag agataaagtc
      481 tgccgggaac tgtttttcaa aaagttcttt attttcaaat ggatagtgat gctgtgcaaa
      541 aggcattgct cctgagtcaa accaacagtc aataacctcc ggtacacgat gcatggttcc
      601 accgcattcc gggcatttca gtgtgatctc atcgatgtat ggacgatgaa gttctactgt
      661 cttggctttt tcgtcaccgc tcatctcgaa cagttcctga cggcttccga tggaatgcat
      721 atgtccacat tcacactgcc agatgttcag tggtgttccc cagtaacggt ttctggaaat
      781 accccagtcc tgaacgtttt ccagccagtc accgaaacgt cctttaccaa tgctctccgg
      841 aatccagtta attgtattgt tgttgcggat caggtcatct ttaactgctg tcatcttgat
      901 aaaccaggat tcacgtgcat agtagatcag tggtgtatca catctccagc agtgcggata
      961 atcatgctcg aatttcggag catcgaaaag ttttccttct ttatccagat cctgaagtac
     1021 aagcggatct gctttcttga caaaaacgcc cgcatatgga gtttctgctg tcatatcacc
     1081 tttaccgtct acaaactgta cgaacggaag gtcataatcg cgtcctacac ggctgtcgtc
     1141 ttcaccgaat gcaggtgcga tatgaacgat accggtacca tcgctcatag ttacataatt
     1201 gtctgctgta acaaaatgag ctttcttttt ctgttttgct gccgcttctc ctgcgcatgc
     1261 gaagagtggc tcatattctt tgtattccag atcttttccg acatatttct caagaacctc
     1321 atatgccggt gtctcttcgg aaccaagttt gccgagaacc ttgtcaagca gtgcttctgc
     1381 catgtaataa gtataaccgt ctgcagcttt taccttgcag taaacttctg tcgggtttac
     1441 gcagagtgca acgttggatg gcagtgtcca cggtgttgtg gtccatgcca ggaagtatgc
     1501 atcttctccg acaactttaa aacgaacgat tgcagaacgt tctttaactg ttttatatcc
     1561 ctgggaaact tcctgtgcag aaagcggagt accacaacgc gggcagtaag gaacgatctt
     1621 gaatcctttg taaagaagtt ttttattcca gatttctttc aaagcccacc attctgactc
     1681 gatatagtca tcatggtaag taacatatgg attctccata tctgcccaga aaccaactgt
     1741 tgaagaaaag tcttcccaca ttcctttgta tttccagaca ctctctttac actgctggat
     1801 gaatggctcc ataccatatt cctcgatctg ttcttttccg tccagaccaa gtttcttctc
     1861 tacttccagc tctaccggaa gtccgtgggt atcccatcct gctttacgag gtaccatgta
     1921 gcctttcata gtacggtatc tcgggatcat atctttgata acacgggtca gtacatgacc
     1981 gatatgcggt ttgccgtttg ctgtcggcgg tccatcatag aacatataag taggatcgtc
     2041 tttacggtcc tccatacttt tttcgaagat atggttttct ttccagaatt tttctacctg
     2101 tttttcacga tctacgaaat tcaggttcgt atctactttc tgatacacaa cagattcctc
     2161 ctttaaattc ttcagaaaaa ttttcatcaa tagtcaaatg aaataaaaaa aacttccgtc
     2221 ctgtaaaagg acgaaagcaa tttccacatt cgcaccacct gttacattgt acgggctggt
     2281 agtataatca tatatgccga tacatgttcc ctttaacggc ggaaacaccg tctctcctta
     2341 cttaagattt caggaaagaa actccggagt gatcttccat cgaaattact ctcaccaggc
     2401 ttccaccttc tcccggctct ctgcagattt ggttccgatt tactgtctcc atcaaagttt
     2461 ttacttatat ctatttgtgc tgttttatta taaggttata tttttccata tgtcaaggtn
     2521 nnnnnnnnna aggtcttttt atgagaatca cggggtttta taaggatttt gcatggattt
     2581 aacccttgta tgcagaaaat gattacagta taattatatg ataatggtat agtggagaaa
     2641 atgatatgct atttaagtat agagaaacca tatcgaatgt atttactttc acgtaactga
     2701 ctttcatgca tcatatttta gaacacggag cttacgtttc cgttttttgg ctgtgcgcct
     2761 attgatgact gcttttgcaa tctcgcttcg ctcaaacagt gcaacgcagc caaaattccg
     2821 aaagcctcgc tggtgttcct aatatgatgc atgaaagtca gttactgaac caccaaaata
     2881 ctagtatgat ttctctatac tcaaatcgcc atcattttct tcatggtcgg cgacggctgg
     2941 gccgcggggc gtgcgtgagc acgtgaacag ttcgaaccct tcccgccccg ccgaatgatt
     3001 gatcaatatt aacaatataa agcctttggt gcagggaagt ttttttactg tgaatgttgt
     3061 tgaatgtaga tgaggctgag aatatctggt tctgggtcgc ctgagagaat ttgtagaagt
     3121 acctgagtat aaatctttgc gttgtatgaa aaaaagacac tgtctgagcg tagcgagttt
     3181 ggcttttttt catacgcttt tagcaaagga atatactcct ggtacttctg aattctcgaa
     3241 ttgacccaga taccagattt ctcatgcatt atataaaatc aaccaacatt acttaatat
//
LOCUS       DAWDWH010000221         3271 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_10558_length_3271_cov_8.813744, whole genome shotgun sequence.
ACCESSION   DAWDWH010000221 DAWDWH010000000
VERSION     DAWDWH010000221.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10558_length_3271_cov_8.813744"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2300..2309
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          940 a          701 c          887 g          733 t
ORIGIN      
        1 acatcaagac aatgatggca aacggtgtgt atgcgatgag tgcgatcacg gcactgacgg
       61 cacagaatac cacaaaagta gcagatatta tggaagtgac accgcagttt ctggcagagc
      121 agctggactg catttttaca gatatccggc cggatgcggt aaagaccggc atggttgcct
      181 ccgcagctct gatccggaca atcgccggga aactgacgga gtatcaggcg gaaaatatcg
      241 tggtcgatcc ggtcatggtt tctacaagcg gagcaagact gatcagtgag gaggccatcg
      301 agacgttaaa agaaaagctg ttcccgctgg caacggtgat cacgccgaac attccggagg
      361 cagaagtgct ggcggatatg gagatcgaga cagtgcagga tatggaaaaa gcagcagaag
      421 tgatcggaac acggtatgga tgctcagttc ttttaaaggg cggtcatcag aaaaacgatg
      481 ccagcgatct gctctggcag aaggataaga agccggtgtg gttccatgga gtgcggatcg
      541 acaatccaaa cacgcacgga accggatgta cgctttcttc tgcgatcgca tccaatcttg
      601 cgaagggcag agatctggaa acttcggtcg aggcggcaaa acgctatatt tccggggcac
      661 tggcagcgat gctggatctt ggatctggca gcggtccgat gaatcatgga tttgcgataa
      721 aggaagagtg gtaatatgga taacgcaaaa aagaaaaata ataagataaa taatcattca
      781 tacaacgaga aatacaacag caggagcagg attgaaaagc tgacactgac ggctctgttt
      841 acggcgattg cagtggtcgg cagtatgttt tccttcccta tattcgggtc aaaatgtgca
      901 cccgtgcagc atctggtcaa cgttctgtgt gcagtgacag tcggaccgtg gtggggactt
      961 ggacaggcat ttttagcggc actgatccgc aatctgaccg ggcttggaag cccgctggca
     1021 ttccctggaa gcatgtgcgg ggcactgctt ggcggacttc tgtatcgcta tgggaaaaaa
     1081 ctgccgtttg cctacatcgg agaagtgttt ggaacgggca tcatcggcgg catgctttct
     1141 tatccggtgg catctctgat catgggaaat caaagtgcag cactgtttac atttgttgtg
     1201 ccgttccttg tcagtacctg cggcggtacg gtcctggcag ctgtgatcac cgtttcgatg
     1261 gcgaaaatgg gtgtgcttgc taagctgcag aacggagaag tgtagatgcg gatggaggtt
     1321 tcagatcaga aaaataccgg aatttttgca gaaaagattg cttcgctgca tcaggaaata
     1381 caaaacaggc gtccgctggt gcagtgcatc accaatgcag tgaccgtcaa tgactgtgcc
     1441 aacattttgc tggcagccgg tgcgtccccg accatggcac atcatccgca ggaggctgct
     1501 gagatttccg cggggtgtga tgcactggtc tgcaatttcg gggcgattgc ggattatgaa
     1561 gcgatgaaaa gtgcggcact tatggcaaca aggctggggc atccgatcgt gatcgatccg
     1621 gtcggtgtat ccggctccac ttaccgcagg acaaaatgta ttgagctgat aaaaaaagcc
     1681 ggggcggact gcatccgagg gaattattcg gagattgcgg cactgatcaa aaatgacaga
     1741 acggttgtcg gggtggatgc catgcagcag cagatcgata cacaggcaat gcaggaattt
     1801 gccagacaga acagactgtt tctgtgggca tccggagaga cggacattct gacggacggc
     1861 gaacgtctgt ggtactgtaa gaacggtgat ccgctgatgg caaggatcac aggcagcggg
     1921 tgtatgtcct cggcactttt aggagccttt ctttcaatcg aaaattcgct ggaaagtgtg
     1981 gcggcgtgca caggggggaa cgatgacctt tcgcctacaa tttatagaca caatttcgct
     2041 tatttcatca caaaacgaaa tatttttaaa gaataagatc ataattccat aatttttagc
     2101 gaataaaaca gggtggatat ttatacaagt tgtgatataa tagactggaa ttgttttcgt
     2161 ggatatcgtt tcgtggaaaa gtctgctttc ggtatcagag gaaaatagaa ttcctgcttt
     2221 taaaaagcag gcacgaaagc tgaatttaga atgaaacaga tgaggagaat gacatgagtt
     2281 tcgaatttat caaaaaattn nnnnnnnnng agatcagaga aaaatatcct ctgtcagaag
     2341 aactggcagc aatcaaaaaa gcaagagatg aggagatccg caaggtgttt accggagaat
     2401 ccgataaatt cctggtgatc atcggacctt gttctgcaga taatgaagag gcagttctgg
     2461 attatgcagg ccgcctggca aaagtaaatg aaaaagtcgg cgacaagctg atcctgatcc
     2521 cacgtattta tacaaacaaa ccgcgtacaa caggtgaagg atataaagga atgatgcatc
     2581 agccggaccc ggagaaaaaa ccggacctgc tggcaggact tctggcaatc cgtaaaatgc
     2641 atattcatgc aatcgagcag accggtctta ccgcagcaga tgaaatgctg tacccggaaa
     2701 actacagata cttatctgac ctgctttctt atgtagcgat tggagcacgt tctgtagaag
     2761 accagcagca tcgtctggta gtcagcggca tggacgtacc ggccggtatg aagaacccga
     2821 ccagcggcga cttaagcgtt atgctcaact ctgttgttgc agcacagtgc agccacagct
     2881 ttatttaccg ttcctgggaa gtgaagacaa ccggaaacga actggcacat accattttac
     2941 gtggagctgt aaacaaacac ggcaactgca tcccgaacta ccactatgaa gacctgaacc
     3001 tggtagaccg tctctataaa gagagaaatc tcaaaaaccc ggctgttgtt gtggatgcaa
     3061 accactccaa ctccaacaaa cagtatgcag aacaggttcg tatcgtgaac gaagtgcttc
     3121 acagccgcaa cctgaatcca cacatcaaaa cactggtaaa aggtgtgatg atcgaaagtt
     3181 acatcgaagg tggaaatcag aagattggat gtgtaccgca catctacggc aaatccatca
     3241 cagacccatg ccttggatgg gatgattccg a
//
LOCUS       DAWDWH010000222         3211 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_10761_length_3211_cov_7.565589, whole genome shotgun sequence.
ACCESSION   DAWDWH010000222 DAWDWH010000000
VERSION     DAWDWH010000222.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10761_length_3211_cov_7.565589"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    290..299
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          849 a          737 c          774 g          841 t
ORIGIN      
        1 catcagggcg atatccgggc ggcagaggaa gttgataaat ttctcagcat tttccttgtg
       61 ctcggcattt ttcgggatca cccaggaatc gatccagaga ttgcttccct ctttcgggat
      121 cacgtactcc agattcgggt tttcctgctg ggtgtagatg gcttcaccgg aataaatgac
      181 accgatcgct gcggctgttc cgatcagttt ttcgatcaag tgtcagataa aaatatgaag
      241 aacccgacgt ttcatacggg tggggtggac gtaggggggg ggcagggcan nnnnnnnnng
      301 gcttcaccgg actaattgac accgatcggg gcttcaggtc cgatcaggtg gtctcggacg
      361 tgatcgatga catatgcctg taccagtggt ttctggcggg tcagcaggtc tttagcttcg
      421 ttcagttcat ccagatccgt ggaatttaag gagtagccga ggtatttgag tgccacgcca
      481 aatgcgtcac ggacggaatc ctgcatcagg atgttgtctt tgtatttctc atcccacaga
      541 acactccagc tgtcgatcgg ctcatctacc atggtcttat tatagaggat accaacggta
      601 ccccagcagt acggaaccga gtatttgttt tccggatcaa actcttttga acgttccaga
      661 tagtctttgc cgatgttttt gatattcgga atattgtcaa aattgatttt ggcaagcagg
      721 tcattctccc tcattctctg gatcatatag tctgaaggac ataccagatc ataggcaatg
      781 gcaccggaac tgatcttcgg atacatgatc tcgtttgtct caaattcttc atatacaaca
      841 tcaatgccgg tttcttcttc aaacagggtg atgacatccg gatccatgta ttcgccccag
      901 ttgtagacga tcagtttttc ttttgtcagc atctgaccct tggaagcata gtaaataccg
      961 cctccgacaa ataccaggca gatcgcgaca gggatcacac gtttgatcag gatcctgccg
     1021 gctctggaac ggcggcggac gtgttttctg gttgccgctg tctctgcgtc ctttttcggt
     1081 gatacgttga ccaggatcag aagtaccaga accgtaacaa acagcagggt cgaaagtgcg
     1141 tacatttccg gtttgatgcc cttacgcact tctgaataga ttttggtaga aagggtatcc
     1201 acaccgggac cttttgtaaa atgtgtgatc acaaaatcat ccagtgacat ggtaaacgcc
     1261 agcataaaac cggagagcac acccggcaga atgtcaggaa acgtcacttt aaaaaaggca
     1321 taaagaggag aagccccaag atccagtgcc gcttcataag tacgtcggct cgtctgtcgc
     1381 agtttcggtg ccacactcag tatgacataa ggtatattga acgtgatatg ggcgatcagg
     1441 attgttccaa atccaagcgt aacgtgaaag gcaataaata aaagcatcag agaaacaccc
     1501 atcacgatct ctgcattcat catcgggatg ttggtcagcc cggaatataa agtacgccat
     1561 ttctgtttca tgccatggat cgcgatcgct gcggctgttc cgatcacggt tgcgatcacg
     1621 gctgcgagca gggagatgat caaagtggtg taaagtgcat tcatgatctg ttcattctga
     1681 aaaaggctgc tgtaccattt cagagtaaat ccgccccatt ttgccctggt ctttgaatta
     1741 ttgaaagaca gcacaatcag agtgaggatc ggtgcgtaga gcaggatgaa gatcaggaaa
     1801 agatatattt tttcgagata tttctttatc aaaacgaaga gccctcccct tctttgtcat
     1861 attttgctac cagtgccatg ctggcaatga taaataccat caggaccatg gaaagtccgc
     1921 ttccgacgtg ccagttgctg ttttgcttga aagactgttc gatgacattg ccgatcagca
     1981 ggatcttgcc tccgccgaga agatcggaaa taacgaaagt tgtcagagcc ggtacaaata
     2041 ccatcgtaat gccgctgatg atgccgggga tgctcagcgg cagtatgatt tttaaaaagg
     2101 tataagaaga gcttgctcca aggtctcttg ctgcattgat gacatcacgg tcaatcttga
     2161 tcagtacatt gtagatcggc agcaccataa aaggcagaaa attgtaaacc atgccaagaa
     2221 tgatcgcccc cggagtgttg atgatctcca gtgccgggag atgcacaaaa gaaaggatgc
     2281 tgttgatcac accgtttttt tccagaagcg tctgccatgc catggtgcgg agcaggaaat
     2341 tcatccacat cggaagaatg aaaatcagca cgataaaact ggactgattg acattcttgc
     2401 tgcttaagat catggcaagc ggataggcaa gcagcagaca gatcgctgta ctcacgaaag
     2461 agaggagcag tgacatgcca agtgccttta aattttcctg tgtggtgatc tgggcaaggt
     2521 ttgcaaaagt gatactgcct tcatcatttg tcatggcata gtagaagacc accagcagag
     2581 ggatcacgac aaaagaaatc gaccagaaca ggtacggccc tgcaagataa cgtttattct
     2641 tcaatgttga cggcctcctc atcttcagat tccggttttt tcatgatctg gatatcaaaa
     2701 ggatctacat ggatggaaac ctgtgtgccg accgggaaca tcttggtgct gtgcaccagc
     2761 cagtcgtatt catttgcacg gacttccatt tcataatgaa cgcctttgaa gatcagatgc
     2821 gtgacaacac cgtcaatgct tccttcaccc ggtttgcgaa ggcagatatc ttccggacgg
     2881 atcacgacat cgaccggttt gttctgtccg aatccttcgt ccacgcaggc aaacggaacg
     2941 ccgagtattt ttaccagacg gtcttttaac atcaggctcg gaatgatgtt gctctcaccg
     3001 ataaagtcag cgacaaaagc attttccggt tcattgtaaa tatgctccgg cgtgccaatc
     3061 tgctgaatat agccctgatt cataaccacg atcgtgtcag acatggtaag tgcctcttcc
     3121 tgatcgtgtg ttacataaat aaaagtaatg ccaagttcgt ttttgaggcg gatcagttca
     3181 tactgcatat cctgacgcag tttcagatcc a
//
LOCUS       DAWDWH010000223         3209 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_10763_length_3209_cov_10.980977, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000223 DAWDWH010000000
VERSION     DAWDWH010000223.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10763_length_3209_cov_10.980977"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1228..1237
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          916 a          640 c          880 g          763 t
ORIGIN      
        1 ggtacgcacg gtggtgtgag aggacggcgg ttagtcaccg cctcctactc gattaagtga
       61 taaataaaag aaaaaacgtg atttacagat cagcccttga aataatgttc tctgatcggc
      121 tcgatcggca tttcttttcc ggtccagagg cggaaggctt cggcaccctg gaaaagcagc
      181 atgtaaagcc cgttaaaggt ggcacagcca tttgcggaag ccatctgaag caggcgtgtc
      241 tgcttcggat tgtaaatgat atccgacacg atcagtttcg gacgaagcat ggaagcatcc
      301 gggatcggac aggtatctgc gtgcggagcc atgccaaggg aagtggcgtt ggtcaggatc
      361 gtactggctt caagtgcctg ccgcagggat gcttcatcag caagatccga tactgcagct
      421 ttacaggaag tgttagcatt gatcgtgtcg gcaagtttta gggcacgttc ccaggaacgg
      481 cctttgcggt taaaaatatg cagtgtggat acaccgtcta atgcagcctg tacggcgatg
      541 gcagttgctg caccgccggc acccagaagt gtcatttctt tgccgatgat gtcgtgtccg
      601 gcctctttga ctgcctgcat atagccgatg ccgtcggtgt tgtgaccgac cagtctgccg
      661 ttttcattga caaccgtgtt gacagcacca atcatgcggg cggcatccga aagatcatcg
      721 atcagctcac agatttttgt cttatccggc atggtgcagt taaaaccggc gatgttcata
      781 catcgcagcc cctttaccgt atctgcaagg tttgcttccg gcacatcgaa acaaagatag
      841 acataattaa gatccagcag gcggaaagct tcattgtgca tcagaggaga gatactgtgg
      901 gaaaccgggc ttccgagaag ccctgtcagc tttgtgtgcc cgtcaatcac aggaaatgta
      961 tcgttcataa gatcacctcg taagtttttg tatttttgcg atatcgtgat gcccgcgaat
     1021 gtttctgctg caaaataaca aaacgtcaga tgccggcatc ccgtcagttt tatgataatg
     1081 accgggcgtg catctgtcaa gtgctggact ttgcggcgga agtatagtat aattacagga
     1141 aaaagcagga atgtatggga ggcacagcaa tatggacaaa acagtacagg tcagaaatct
     1201 cgtgatcgga gagggaagac cgaaaatnnn nnnnnnnacc gaaaatctgc attccggtca
     1261 tgggcagcac gacagaaaca ttaaaagaag ctgccggtca tgcaatgaaa aaacatccgg
     1321 atctgattga atggagagcg gattattttg cggatatcga gaacgaagaa gtacgcagac
     1381 aggcatttga tgagctgaga gagattctgg gagaagtacc gttgctgttt acgatccgta
     1441 caagaagaga aggcggagag gctgatcttt cggcagacca gtatctcaag gttgtatcgg
     1501 atgcgatccg ggatgaaaag gcagatctgg tggacgtgga aatctcccac ggcgatgaca
     1561 tagcatttat gctgatctcg cttgcacatg agtttgggat caagaccata gcatccagcc
     1621 atgatttttc acagacaccg aaaaaggagc agatcatcat gacactttgc aagatgcagg
     1681 aattagaagc cgatatcccg aagatggcgg tgatgccaca gagagagaga gatgttctgg
     1741 tactgctcga tgcaacatta agtatgaaag agctccatgc ccagacaccg gtcatcacga
     1801 tggccatggg aaggaacggt gtggtatcaa ggctgtcggg agaagtattc ggctctgctg
     1861 tgacatttgg gacggcagga gcacagtccg caccgggaca gatcgatgcg gaagaattaa
     1921 ggaagatttt ggaacttttg gccgtcgtct gatgtcaacg aaaaaacaga acacaagatc
     1981 gcagttttga caaaatatgc accccttgtc acctataatg gtaagtgctt aagcggcact
     2041 ccatgtagga ggcagggggt gttttgtgta ctatgaagtt tatctggatt tttatttttt
     2101 agaaagtttt ttaacggatg tggctctgct tatcctggta aaaacggtga tgcgggaaaa
     2161 atggaaggca aaacgtattg caggcacagc cgtgctggaa agcatgtggt cctgtatcga
     2221 attgctgctt ccctttcggg ggagttttac agcgaccttt ttctggatcg tttccgtttt
     2281 gcttctggta aggggaggac ttggaaaaag aacggcaaaa gaagtgctct atggtgcatt
     2341 gttgcttgga atttcaagct tttgctttgg cggtgcactg tatgtggcag aaacgttccg
     2401 atatattccg ggaatttttg tgaaaatggc ggtatttctg atcctgtgga tgcttttgta
     2461 tctgtatacg gaaaccgacc aaaacagaca gcggatctgc gaggtcagtc tctggcagtg
     2521 tgggaaaaat agaaaagtga aggcacttta cgatacggga aaccgccttc gagaacctta
     2581 taaaaaacgt ccggtaaata tcatagagta cgaagcggca aaagagctgc tggatggaaa
     2641 agaaaatgtg tttttgattc cataccgtac cgtaagcggt tcaggggaaa tgctcagagg
     2701 gatagttttt gacagaatga ttgtcagcaa aggaagaaaa acagagattt atgaacatcc
     2761 ggtgatagcc cttacggggg aacgtgtgag cagtgatggc agttatcaga tgatactgca
     2821 tccagataac cggaaaaatc aggaggagaa ggattatgtt taaaatggcg accccacaga
     2881 aatttcagtt tcgaatgaca aaaggatggc agaatatatg gttccgcaga acaaacgaag
     2941 tgcattatat cggaggcagt gatgtgctgc cagcaccact tgaggcacag gaagaggcaa
     3001 aaatgctgga aaacctggaa ggcggagaac atcaggaaga ggccagaaaa cggctgatcg
     3061 agcacaattt gagactggtg gtttacatag cgaaaaaatt tgataatacg ggcgttggag
     3121 tggaagatct gatatccata ggaacgatcg gactgatcaa ggcgatcaat acctttaaca
     3181 ccggaaaaaa tatcaaactg gcgacgtac
//
LOCUS       DAWDWH010000224         3149 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_10914_length_3149_cov_6.781836, whole genome shotgun sequence.
ACCESSION   DAWDWH010000224 DAWDWH010000000
VERSION     DAWDWH010000224.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10914_length_3149_cov_6.781836"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1008 a          550 c          744 g          847 t
ORIGIN      
        1 aggagaaagc tggaagatgt ggttcagtgg agaagataga ctgagaaagg gagccaaagg
       61 agatggaaga acaaaagcag gcagagcagc aatggaataa aatccgccgg gagcgaataa
      121 acattttgaa agaggctcca tcagaggaga atctgtggca ggcagtcatg gcatttcagg
      181 attatccatt caaaacagta acggggctgc cgtttcaata tacactaaaa acaggcaaaa
      241 acggagaatg gacaaaagag ttatggatcg accgcagaga aaagagcaaa agtctgtcgt
      301 ggagcagcgt ggtactggca tttaaaaaca gccggaaaac aacagaaatt gtgaaacggc
      361 cgaaagcact tggagatatc cgtgggattt cctatattta tccgattttg tggagactgg
      421 gcctgatcag ggtaccggag aaatttgaag aaaagatggc atgcgatgat gaaaagaatg
      481 caaaagggta gatgaggttt cgggcggtca gccggaaatg ctgtgatcgt cctgagaagg
      541 attcctgttt tctaaaaact gccggaggca ccgctctgat ctggcaccga ctaggataca
      601 gcagtagaga atcagaatat acaggaaaag aatcaaaatg ccgatagtga taagagtcag
      661 tttcatggta tcattgtcct ttctgaaagt atagtaagct gcgttcgacc tgaacgcaaa
      721 gagcatatat atcgtgtctg aagagagctt tttctctcga accatgttag tataagtata
      781 tggagatgcc agggaaaata tgtatctggt tatgagcaag taacaaagcg gattgcgaat
      841 atccgcttga cagatataaa aggagaaatg gaaaatttga ttgaattatt aaagaaaatc
      901 ataacaaacg tcctgacggc actttatcag ccgttctggt ttgcagttat tctgactgta
      961 tttctgttgt ttttttatct ttatgcctat aagccgattg acacaggaaa gggcatccgg
     1021 gcagcatttc gggcatggtg gcaggaattt cgaacttcgc tgttctttcg aaaactcttt
     1081 ttgctgacgt tttttacagt gatgatcctg tttcagacac tgatcaaccg aaatatgtgg
     1141 atcaatccgt tgtcggatat tatgggaggc tggtggatct ggcacacggc ggcagacggc
     1201 acggtcacac tgacgacgga atgctttgaa aatatcgtac tgatgcttcc gtttacggca
     1261 ctgcttttgt ggacagtggc agaaaaaata ttttgcggca gaaagataac gtttgggaga
     1321 tgtgtatgga tatcaacgaa aacagccttt ctgttttcgc tggcgattga gtttttacag
     1381 ctgtttctaa gacttggaac atttcagctg tcagatttgt tttacaatac tctgggtggt
     1441 gtattaggtg gaatgattta ctggatctgt tggaaaatcc gggggaaaaa acaaaaaaca
     1501 ggaaaataag aaaaatcaga ataacagatc aaatggaatc cggacagagc ttcggatcct
     1561 ttttctttac ataaagatgt acagatgagc aaatcgggag gttgaaatat tcaaacctcc
     1621 cgacctacaa caaaatttga gaaatataaa aatatggatg acattattat attacgcttt
     1681 tttcgatttg taaagagcaa atataccaaa acttacaaaa aatattgcgg aaactgcaat
     1741 tggccatgtg gaacgggcac cggtctgcgg tgaaacctgg cttgttccaa tggtcgcacc
     1801 cttttcacgg tagatcagag ccataacaga tgaaccggca tggaaataag cggctacctg
     1861 gttatcactg atcttttctc cctgaagaac ttcccagcca tgctccggac aatagtagag
     1921 gatatccacc tggatgttgt ccgtcatatt atttgctaca aaagtaactc tgaccggatc
     1981 atcagctgtt ggtgtcacac tgtcaaaagt aatatccatg accggagaaa ggaatttgta
     2041 cagcccaagg tcgatgcccc ttttgataat gctgttctgt ccataaagat tgatcggact
     2101 tggaagatca tcgccaaaag catcttttac agtagtacct cgtctggcat tgttcagacg
     2161 atcgatgatt cgttgtaatt cttcactgta tttgcttaaa tctacagcac ttaaagttac
     2221 tttgccgcgg tcagaagatg cattcggcat atcaatcgag ccgacaatgc ttccagcagc
     2281 gaagacattc aaagataaag caagaactgc gatgacagct gtgaatgcct ttaagaaagt
     2341 ttttttcatt tatcacgcct ccatttgaaa tttaatatac atcaataaac gattgcttat
     2401 cgcttatttt aacacgagtc ggcagaaatc gcaagtaaga accgtatttt aacaggcaaa
     2461 acatatctat ctgctgattc aaaaattaag aaaattttta taatatcttg tcaagtacag
     2521 aatgatagta tataatgaaa aacgattaaa gaagaccgag acggaagaaa aagaagatca
     2581 agggagcgtg cgagtatgaa aaacagcaaa atacagaaac tcaaaaaaag ggggagtaca
     2641 cttcttcttc tggttttatt tatgtgtgtt ttagcaatgg tagtggctgt gattttggtg
     2701 agcagccctg cggttgatgc acagtataaa actgtaggct gcatggtgat tgataagaag
     2761 aatgcagtaa ttgcggcgat ggcggcagtg ttctgtggta tagcaggatt tgtgatccga
     2821 catcatgcgg caagatgatg gaaactgact gttgaaaaat agtcagtttt ttgacgaaca
     2881 gagcagaaat gaaacagaac aggagatatt taaaatatga attacatcag tcaacaagaa
     2941 ccaaatcaaa tgtggatcaa aaaagtattg atgctggtac tggcagacat attgattgta
     3001 ttgatttcct attttatggc tctgcttttg agatttgact ttgtgttttc aagtatcccg
     3061 gaagaatatc tgatgggata tctgtggtcc atgcctgcaa attatgttca aacggaatgc
     3121 cagttccgct actgcggaac ggctattcc
//
LOCUS       DAWDWH010000225         3109 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_11065_length_3109_cov_10.425999, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000225 DAWDWH010000000
VERSION     DAWDWH010000225.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11065_length_3109_cov_10.425999"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1450..1459
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          946 a          607 c          815 g          731 t
ORIGIN      
        1 cttttttctt tgccccataa gagaacattt gttttggggt gtgataagaa caagcatgtt
       61 tttgagcaag gaggaaaata tggcaaaaaa aagcacctat gatgccagca gtatttcggt
      121 actggaaggt ctggaggctg tcaggaagcg tcctgggatg tatattggca gcgtttccag
      181 aaagggtttg aatcatttaa tatatgaaat cgtagataac tcggtggatg aacatctggc
      241 tggtttttgt gaccgcattg aagtgacact ggaaaaagac ggctctgcaa ccatcaaaga
      301 caatggacgt ggtattccgg tgggcatgca cgaaaaaggc atgtcggcag aacgtctcgt
      361 tttcacgacg ctgcatgcag gcggtaagtt tgataattcg gtctataaga caagcggcgg
      421 tctgcatggt gtcggatctt ccgttgtaaa tgccttgtcc gaatggctgg aaataaaagt
      481 aagccgtgaa ggttgcgtgc actatgaccg ttacgagaga gggattccga cggtcgaact
      541 ggaaaacggc ctgctcccgg ttattgcaaa gaccagagaa acaggaacct ggattcgttt
      601 tcttccagat gcagagattt ttgaaaagac tcgtttttcg gcagcagaag taaagagcag
      661 gctgcacgaa actgcttatc tgaatccaaa tcttacgatt gtattcaagg atctgcggga
      721 tgaggagaaa gagacggtca cgtttcatga gccggacggt atcgtcggct atatccggga
      781 tttaaataag aaaaaggaaa cgatccacga gccggtttat ctgtgtggag aagcggatgg
      841 cattcatgta gaatgtgcat ttcagtaagt caatgaattc catgaaaacg tacttggctt
      901 ctgcaataat atttacaatg cagaaggcgg cacgcacctg accggtttca aaaccgtgtt
      961 taccacggtg atgaacactt acgcaagaga gcttgggatt ttaaaagaaa aggatgccaa
     1021 ctttaccgga gcagatatcc gaaacggtat gacggcggtt gtttctataa aacatccggc
     1081 tccgcgattt gaaggtcaga caaagaccaa gctggataac caggatgcct caaaagcaac
     1141 ggcgaaggtg gtaggagatg aggtggtccg ctattttgac cgaaatctgg aagtattaaa
     1201 gagcgtgctc tcctgtgcgg aaaaagcagc caaaatccgc aaaacagaag aaaaagcaaa
     1261 aacaaacatg cttacaaagc agaaatattc ttttgattcc aacggaaagc tggcaaactg
     1321 tgaaagccgc gatgcttcaa aatgcgaaat ttttatcgtg gagggagatt ctgcgggggg
     1381 atctgcaaaa acggcaagaa accgtaattt ccaggcgatc ctgccgattc gcggtaagat
     1441 tttaaatgtn nnnnnnnnna taaggttctt gctaatgccg agatcaaaac aatgatcaat
     1501 gcatttggct gtggattctc agaaggatac ggtaatgatt ttgatatcac aaaactccgt
     1561 tacgataaga tcgtgatcat ggcagatgcc gacgtggacg gtgctcatat ctcaaccctg
     1621 cttctgacgc tgttttatcg ttttatgccg gatctgatct atgaaggaca tgtatatatt
     1681 gcaatgccgc cgctttacaa agcgatgcct tccagaggag aagaagagta tctgtacgat
     1741 gataaagcac tggagcgtta ccgcaaaaca cataaaggca atttcacgct gcagcgttac
     1801 aaaggtcttg gcgagatgga tgccgagcag ctctgggaga ccacactgaa tccagaaaca
     1861 cgtattctga aaagggtgga gatcgaagat gcacgtatgg cttcggatgt gacggaaatg
     1921 ctgatgggaa gcgatgttcc accaagaaag gcatttattt atgaacatgc ccaggatgca
     1981 gaactggata tatagaacga aacaaaatat agaatcgctt cgaacagatg aagcatctga
     2041 tggcagaatc acaagaacag gattgaaata aggagaaagt tacgacatgg atagagaaca
     2101 ggaacagatc atccggacag agtattcgga gctgatgcaa aaatcatata ttgattatgc
     2161 aatgagtgtc attgtctcaa gggcactgcc ggatgtgcgt gacggactga aaccggtaca
     2221 gagacgaaca ctttatgata tgtatgaact gggcattcgc tatgaccgcc cttatcgtaa
     2281 atgtgcccgt attgtcggag atacgatggg taaatatcat ccgcatggcg acagctcgat
     2341 ctatgagtct ctggttgtga tggcacagga ctttaaaaaa ggactgcctc tggtggatgg
     2401 tcacggaaat tttggctcga tcgagggtga cggtgcagcg gcgatgcgat atacagaggc
     2461 acgcctgcag aaagtgactc aggatgcatt tctctcggat ctggacaaag atgtggtgga
     2521 ttttggacca aactttgatg aaacagaaaa agaacctcag gttcttccgg tgcggcttcc
     2581 gaatcttctc gtaaatggat cagaaggaat cgccgttggt atggtgacca gcacaccgcc
     2641 gcacaatttg ggcgaggtga tcgatgccgt caaggcaacg attttaaatc ataagatcac
     2701 gacggaagaa ctgatgcagt atattcaggg tccggatttt ccgaccggcg gcattgtcat
     2761 caacaaagac gatctgcttt ccgtttacga gacagggaca ggcaagatcc gcctgcgtgg
     2821 acgtgtggag atcgaaaagc tcaaaggcgg aaaagaacgc attgtgatca cagagatccc
     2881 atataccatg atgggggcaa atatcggcaa atttttaaat gatatcgcag ggctggtaga
     2941 gaacaaaacc accacagata tcgtcgatat ctccaatcag tcctcgaaag acggtataag
     3001 gatcgttctg gagctcagga aaaatgcaga tacgcagaat atcatcaata tgctttacaa
     3061 aaaaaccaga ctggaggaca cgttcggtgt caatatgctt gccgtggca
//
LOCUS       DAWDWH010000226         3099 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_11100_length_3099_cov_10.449080, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000226 DAWDWH010000000
VERSION     DAWDWH010000226.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11100_length_3099_cov_10.449080"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2341..2350
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2688..2833
                     /estimated_length=146
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          804 a          685 c          645 g          809 t
ORIGIN      
        1 tccgaataaa aacagtgcta ctccacatag aagtgataaa atcattgaaa atatctgcat
       61 agttttctcc tctgtatctt tttcttttcc ttgtccttgt cctcgtattc gttttcctat
      121 tttgattttt aagcatttac ttttccatta tgccacatta tgcgatgaat tacaatattt
      181 ttgcttaagg aaacctttat tttttgtttc taaaaaaatg cgaaacatca tcaaaacgtt
      241 tgatatttca aaaaatttgt cgtataatga actttgtatc ttttttcggg tatcttttcc
      301 tattataata ggtggatcag aaaacggctg ttttctgtcc ggatcccgga tcaaacatca
      361 ttacataagg attttaaatt tatgaatatg aatacgaatc agacaaaact tcatacttta
      421 ggaatcgata tcggttccac aaccgttaag gttgctattt tgaatgagaa aaacgaactt
      481 cttttctctg attatgaacg ccattatgcc aatatccagg agacgctctc tgcacttctg
      541 aaaaaagcat atgacagcct cggtgaactt tcgctctcgc cgatgatcac cggctccggc
      601 ggtcttaccc ttgccaagca tcttggtatt ccttttgttc aggaagttat tgctgtttct
      661 actgctcttc aggactatgc tccgcagaca gatgtagcga tcgagcttgg cggtgaagat
      721 gcaaaaatta tttattttga aggtggaaat gtagagcagc gtatgaatgg tatctgtgcc
      781 ggcggaaccg gatcctttat cgatcagatg gcttcgcttc tgcagaccga cgcttctggg
      841 ctgaatgact atgcaaaaaa ttataaagct ctgtatccaa tcgccgcccg ctgcggtgta
      901 tttgctaaga cagatattca gcctctgatc aacgaagggg ctacgaaaga agacctgtcg
      961 gcttctattt tccaggccgt tgtaaaccag acgatcagtg gtcttgcctg cggaaaaccg
     1021 attcgcggac atgtggcatt tttaggcgga ccgcttcatt ttttaacaga gctgaaaact
     1081 gctttcatcc gcaccctgaa gctggatgac gaacatgcga tcacaccgga aaattctcac
     1141 ctgtttgctg caatcggttc agcccttaat tataaggagg atgttataac atccctttct
     1201 gcactgatcc agaaactttc cggcaccatc cacatggaat ttgaggttgc ccgtatggat
     1261 cctttgtttg cctccaaaga agaatatgag gaattttcgg aacgtcagag tcagtataac
     1321 gttatgaccg gcgatctgga aacgtacgaa ggaaactgct atcttggtat tgatgccgga
     1381 tctaccacga caaaagctgc tctggtcgga gaagacggtg ctctgcttta ttctttctac
     1441 agtaacaaca acggcagccc gttaaagact acgattcgtg caatccagga tatttacaaa
     1501 aagctgcctg acactgccaa gatcgtccgt tcctgttcaa ccggttacgg cgaggcactg
     1561 atcaaggccg ctctcctgct cgacgagggc gaagtagaaa cggtatctca ttattatgcc
     1621 gctgcattct ttgatccgga tgtagactgc atcctggata tcggtggtca ggatatgaaa
     1681 tgtatcaaga tcaaaaatca gaccgttgac agtgtacagc tgaacgaagc atgttcttcc
     1741 ggatgcggtt cttttatcga aacgtttgcc aaatctctga attacagtgt acaggatttt
     1801 gcaaaagccg ctctttttgc caagaacccg atcgaccttg gtacccgttg caccgtattt
     1861 atgaactcaa aggtaaaaca ggctcagaaa gaaggggcag aagtcgctga tatttctgcc
     1921 gggcttgctt attcggttat caaaaatgct ctttataaag taatcaaagt ttccgatgct
     1981 tctgagcttg gcaaacacat cgttgtgcag ggcggtacgt tctacaacga tgccgttctt
     2041 cgcagtttcg aaaagattgc cggatgtgaa gcaatccgcc cggatatcgc cggtatcatg
     2101 ggtgctttcg gtgctgcact gatcgcccgt gagcgttatc aggacggtgt agagaccacc
     2161 atgctctcca tcgacaagat caatcatctg cagttcacga ccaccatggc aaaatgcaaa
     2221 ggctgtacca accagtgccg cctgaccatc aaccgtttta ccgggggccg tcagtttgtg
     2281 accggaaacc gctgtgagcg tggtatcgga aaagagcgaa ataaagagaa aatcccgaac
     2341 nnnnnnnnnn tgttcgaata taagaacaag cttctgtttg accgcaaacc actggaggca
     2401 gatgaagcag taagaggtac cgtcggcatc ccgcgtgtcc tcaatatgta cgaaaattat
     2461 ccattctggt ttgaattttt cacggctctg aaatatcacg tggtactttc tccgccatcc
     2521 accagaaaga tatatgagct tggcatcgag tccatcccaa gcgaatccga atgctacccg
     2581 gcaaagcttg cccacggaca tgtaacctgg ctgatcaaac agggagttcc tttcattttc
     2641 tacccgtgta ttccatatga gcgaaatgaa ttcccggact ctaacaannn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnntgaaaat ccgttcctct cttttgccag tgaagatacc atctgcaaac
     2881 agatgatcaa agccctccct gacattccaa aagaggaagt ccgtgccgct gtaaagactg
     2941 gatggcagga acttgcccgc acaaggcagg cgatgtacga caaaggcgaa gaaactttaa
     3001 aatggatgga agaaaacggc cgccacggca ttgtgcttgc aggccgcccg taccacatcg
     3061 attctgagat caaccacggc atcccggaac tgatcaatt
//
LOCUS       DAWDWH010000227         3085 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_11143_length_3085_cov_10.389109, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000227 DAWDWH010000000
VERSION     DAWDWH010000227.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11143_length_3085_cov_10.389109"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1950..1959
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          713 a          789 c          662 g          911 t
ORIGIN      
        1 ccctgatctg caagaatata acaaagtccg ccctgcggga tcacaccaac cacgcgtgcg
       61 tcggttgact tctcttcccg cacattcagc tcattctcct ttgcgaccgc attgacacgt
      121 tttacaaccg tctcctgaat ggtctttttc acgctggcat aagccggatt ctccttcgga
      181 tcgatttttg ctttcgcaag tgtcataaaa ccttcttttt tgagtgccac tttgactctt
      241 acgacctcac cggtcagaac gtttttattt tttacataac cacgaacatt cccggactca
      301 acataagacc agttttttcc tttccagagt atataacaga gtccgtcctt tgcaagggtt
      361 ccggcaacgt ctgcatggct gtttttcgct gtatagatct taagatccgc ttttgcgaga
      421 gcgtattcct ttgctaccgt ctcaaaacgg aacgactttt ccagttcttc cggatcgatc
      481 accagctttt caaaacgttc atcgaacttc tgctccgaca tggcatcctt ctcactcttg
      541 gaagaatcct ttttgtcttt cgccttttct ttctcctttt cacgctgtgc tgccttttca
      601 tccaggtcct gatctttctc ttttgtatcc tccggatctg tctcatctgg ctcagactgc
      661 tgcgtttcgg tctgtatctc ttcggactcc tgtgtctctt ccgcctttgt atctgccgtc
      721 gtctcactct cggcttccgt agctctggtc tccggctgcg gcggttcact ctccgtgctc
      781 tccgtgttct ctgtatcctg tacagaatct gttgtatttt cagaatcctg catcgtctcc
      841 tgtgcggtgt ccgctccctt atccgaggca tctacgccga ccggaaatgt cactgcaagg
      901 gacatcccga gaagcaatgc aagcatgctc tttctgttat ttaacatcgg tactcctttt
      961 ctccccatag ttttttcatt atagcacttt tacctgcaaa ttgcaaatct gggtgctgca
     1021 gcgagattct gctttccatc tgctgctatc tccggtggat tgcagcgaca ttcttccttc
     1081 agcgattatc atgtcaaggt ctgatatttt ttatattttc tgcaaaatcg catatttcta
     1141 ttcgaaacgg agaattcttt tatcaggaat ataaatcagg aggatttcga atatggcact
     1201 taataaagtt gagatatgtg gtgtgaacac agcgaagctt cctatgctta cgcaagaaga
     1261 aaaagatgct ctttttatta aaatcaaagc gggtgatcag gcagcaagag aaacctttat
     1321 caaaggaaac ctgcgtcttg tgctcagcgt gatcaaacgt ttcggtgcaa gcaatgaaaa
     1381 tgcagatgat ttatttcaga tcggctgtat cggtctgatc aaatccatag ataattttga
     1441 tccttctctc atggtaaaat tctccaccta tgcagtaccg atgatcgttg gggagatccg
     1501 aagatacctg cgggacaaca gtgccatgcg tgtcagccgt tcgcttcggg ataccgccta
     1561 caaagcgatc tatgcccggg aggggtatat gaaaaagaat ctgcgagaac cgacgatcaa
     1621 cgagatcgcc tcagaaatcg gaattgcaaa agaagaaatc gtctacgcca tggatgccat
     1681 ccagacgccg gtcagcttat ttgacccggt ctataccgaa ggcggagatt ctctctacgt
     1741 gatggatcaa atcagtgata aaaagaataa agaagaaaac tgggttgaat caatctctct
     1801 tagggaagcc gtagaacggc ttggtgagcg ggaacgccat atcattaagc ttcgcttcta
     1861 tgaaggcaaa acccagatgg aagtggcaaa tgaagtcggc atctcccagg cacaggtcag
     1921 ccgcctggag aaaggggcgt tgaaggcgan nnnnnnnnnc taaacagaag agccgtatca
     1981 ctgcaaaact gatcgatctg acggtagcaa ggcttcctgt ttacgtataa tcaatttctt
     2041 tatttctgct cttcaacctc tactttacgg ttttcgcggt ttttgatctc ttctgtgatc
     2101 gctgcgataa agtcatccag gctctttgca ccttcatctc ctgcgaaacg gcttctgaca
     2161 gaaacggtat tttcttcttc ctcttttgcc cctactacca gcatataagg aatcttctga
     2221 agtcttgctt cacggatctt gtatccgatc ttttctgctc tggtgtcgat ctcagtacgg
     2281 atatttgctg cttccagtgc tgcttttact ttctcaccgt attccatgta tttatcggag
     2341 atcggcagta cttttacctg taccggggat aaccaggtcg ggaatgcacc tgcaaaatgc
     2401 tcgatcagga ttccgatgaa acgttcgatg gaaccgaatg ctacacgatg gatcatgatc
     2461 ggacgatgtt tctcgccgtc tgcaccaatg tattcgcagt tgaatcgcag cggcagctgg
     2521 aagtcaagct ggatcgtacc gcactgccag gttcttccga tggagtcttc cagatggaag
     2581 tcgatcttcg gtccgtagaa tgctccgtct ccttcattta ctacatacgg cagtcccaga
     2641 tcatccagtg cacctctcag tgcttccgta gccatctccc agtcttcgtc acttcccatg
     2701 gaatcttccg gacgggtgga cagttctaca tgatatttaa atccaaacag ctgatatact
     2761 tcatcgatca gtcttgctac acctttgatc tcgtcacgga tctgctccgg catcataaag
     2821 atatgggcat cgtcctgtgt gaagcagcgt acacgcatca gtccgtgcag ctgaccggat
     2881 ttctcatgac gatgaaccag tccaagctct cccatacgca gcggcagatc acggtaagaa
     2941 cgcggttctg actggtatac caggattccg ccagggcagt tcatcggttt gattgcgaaa
     3001 tccgtgtcat cgatgacggt tgtgtacata ttttccttat aatgatccca gtgaccggat
     3061 gtctcccaca gatggcggct aagca
//
LOCUS       DAWDWH010000228         3084 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_11147_length_3084_cov_11.023110, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000228 DAWDWH010000000
VERSION     DAWDWH010000228.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11147_length_3084_cov_11.023110"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          814 a          816 c          637 g          817 t
ORIGIN      
        1 attcgccgtt gcacagaccg gatttaacgg ataagaaacc agcatgtatt ttgctgcttt
       61 tgccgtctct tccggaatat cggaaagctt cggcagaaat ccgttttcac ggtacaaagg
      121 ataagtacgc acatccgcac cgcacagaga cggaccgatg ctgaagatcg gataccccgg
      181 attcggagtt aagaccacat cgcccggatt gcagagtgcc caggcaatat gagccatacc
      241 ttcctgtgaa ccgttgatcg tcatgatctc ttccggctca atggatacac caaatcttct
      301 cttgtaaaaa ttctgcactg cctccagaag aaatgggcgg tctgtcagtg catatttata
      361 attttccgga tcttttgccg cctctgaaac agcttccatg atatgaggtg ccggcttgaa
      421 atccggtgtg ccgacggaaa gattataaac tttctttccc gccttcacca tctcttcttt
      481 cttttcattt aatattgcaa aaattcctgc ctgaaagctg tctgctttct tcgagaactc
      541 aaattccatg atcctctcca tctccttctt gtatatctcc tgtatctgcg tccgcctgat
      601 ccgcagatac ggcttcttct gctgtttcat ctttctcatc cgcatctttt tctgcctgtc
      661 cgttttcaaa gtcatattct tcaaacgcat cacccgcaga tgaaagttcc ggctttttac
      721 cttttctgat ccagatcgca ctggtcagca ggtaaagtgc cacacagatc aggctgatcc
      781 aggtaccctt ccagagtcct tccggcgtat attcaaacgt gatcgtgtgt gttccttctg
      841 tcacatcaac accggtcagt gcatctccga tcatataata atcatcgtct tcgccgtcga
      901 tcttaacgct ccatccttca tcatatggaa gcgaaagcat cagaacgccg tcttcggccg
      961 cttccaccgt tccttcaaac tttccgtcct caaattttgt gagctgtaac tgagatttgg
     1021 ccagctcatc atgtacctgc tgatagagtt cctgatcgca ggtccacact tccgctgtca
     1081 ccggtccgct ctggttttcc ttaaaggtgc aggtgactgt cgcattgtcg attgcatcaa
     1141 aacatccgag gtcatacaga tgatcgttat aagtgtcata tgttcttgta taatccggtg
     1201 tcgtgatctc aatgctggac acagatgagg tcacatccag ataaacctgt ttgcccgccg
     1261 gaagcacgat tgtataagtg ccgccgtctt ccatgtcgat cgtctcttcc agttcaaaaa
     1321 gatcttcatc cataccggtt gccatcgtca cgaaatcatt ctgtacctca aaatgatcgg
     1381 tgctgtctgt cgtccagtca ataatgtcat cttttaccat aaatccaaga ctcaatgccc
     1441 cgctgttctt atacagattc atatcacctt cctgatcgac tttctccatc tgatagagct
     1501 gattggttcc gcggctcgag agcacatatt ttacgccgaa aatatcattg accagtttgg
     1561 taaatccatt gtaaccgttt ttattcgttc tcgcctcaat accgaggcct ttgcacatat
     1621 caaccgtagc tgccggcatt gtagaggaaa acagaaccac gccgtccgct ccgaggaaca
     1681 tattctcgtc acgcatacgg ttgctgtcaa tctcgcttcg atagaaatta tcctgatttc
     1741 cctgtctgcc ttcctgtcgt tcggttgttt tttcataggc aatctgctca ttcagatagg
     1801 tacttcgtcc cacagttccg ttgcagaata caccaaagat cgcataactt accatttcca
     1861 ccgctgccag gaaaaagaaa agagagcgga agacctcccg gtgcattttc ccaaggcgat
     1921 agatcagcat cgccatacca tagaggatca gaagtccgat ggtgatgcca tacgtataca
     1981 gttcccgttc gccaagtccg gtcactgcag aatagatggc aaacgcaagc ggtgctgcca
     2041 tcgccagaag aatctgccat accttaaact tatgcatatg cctccaggca tcaaacgcca
     2101 tcgtgagcat caggaaaata tagataaatg caaaacggtt cggcagtccg ttctgtacat
     2161 ggaaaccatg ccagatatag ttgagcacat tgacatcaaa gcttgcatag agcagcacaa
     2221 gcagtgccgt ctttgcgatc cgttcacgca gcttgatatt tttatccagc agatacagaa
     2281 cagccaggat cagcgtcacc gttccgcaga atgcattgac accgatctgg tcgtccgcaa
     2341 tgttgatcgg ttcgcagaat gcaaactggc tcgtcagctg cgataaatta ttgacaaaca
     2401 gcttgatcgg gcttggaaaa ctgttctccg cagactccgt aacgccaagt gcgacatagg
     2461 ccggcagcaa taccacggca gccatacctc ctgcaagcag ggcatgccag ccgaaattga
     2521 ctgctgaagt aatgatgttt ttgatcttga tctctcttgc actgatccac agaaccacaa
     2581 aataaaggca ggaaaacagg cagagcataa agccgatgta atagttgcag tataatgcat
     2641 aaaacaggga aatgcagaac agactacccc tctctccttt taaaatccgc tcgatcccca
     2701 tcatcacaag cggcagcatg gcaatgctgt ccagccacat caggttgaaa tagtatccga
     2761 tgataaagct gccaagagag aacatcagcg agaacacaag cggcatgtaa gtctggcctt
     2821 tgctcttcgt actgaaatac caggcagagg taagaccaca aagtccaatc ttgagcacga
     2881 tcagaagtgc catcgtatcc atcatatttc tcttcgggaa aagcacaatc aggaaattca
     2941 acgggctcgc cagataatag gcatacgttg cccagaaatt aaagcccagt cctcctccaa
     3001 aagaatagcg aagggaactt ccctgttcca gtttttcatg gaattccgta aaaaacggaa
     3061 gatactggtg gagtgaatcg atga
//
LOCUS       DAWDWH010000229         3083 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_11156_length_3083_cov_10.905218, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000229 DAWDWH010000000
VERSION     DAWDWH010000229.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11156_length_3083_cov_10.905218"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          737 a          809 c          664 g          873 t
ORIGIN      
        1 aatgcctgta ccactttctg tccttcgatc atctcattga taaatgcagt ctgctcgccg
       61 cggacttcgg tctgcatatg gaaattacga tacgttttct ttgcgataaa acctgccact
      121 ataaagctga gcggtgtgac gcacacaacg acgagcgtga tcggcacatt gacatacagc
      181 ataaaaccca gtgttccgag gatcgtcaga actccggtaa aaaactgggt aaatcccatc
      241 aaaagtccgt ctgcaaactg ctctacatcg gcgatcacac ggctgaccag atctccggtc
      301 ggatgtgcat ccagataaga aaccggcagt ctctggatct tggcgatcgc tgcatttctc
      361 aggtcacgac tgacattata agtgatcttg ttattgcaga taccaaagat ccactgggag
      421 atcgccgtca cggcagtcgc tgccacgatc acccgtacga tcgccgccac acccgcaaaa
      481 tctacttttc cgactccgat catatggtca atagcatttc cggtcaggat cggcacatag
      541 agctgggatg caacagaaag agcggcaaaa atcagactgc atacaatata aaaccagtat
      601 tttcgaatat gtttccagat tctcatgatc atgccgtctc acctgccttt ccaaactgac
      661 tttcataaat ttcctgataa accgggcagt ttccgagcag ttcttcatgc gtgcccatgc
      721 cgacggtctt gccgtcatcc agcaccagga tcagatcggc atgcatcaga ctggacgttc
      781 tctggctgac gataaatacc gtcatctctt tatccaactc acggattgct tttcgaagtg
      841 ccgcatccgt tgcaaaatcc agtgcacttg cactgtcatc caggatcagg atctccggat
      901 cttccaccag agcacgggcg atcgtcagac gctgtttctg acctccggac agattgcgtc
      961 ctccctgctc caccggttca tccagctgtt tctctttttt ctgtacaaat tcggctgcct
     1021 gtgcagtctc aagagctgcc cagagcatct catcggtcgc tcctggattg ccccaaagaa
     1081 ggttcgaacg gatcgtaccg gaaaacagct gtgctttttg catgaccata ccaatcttgc
     1141 cacgcagctc ttctcttgaa tagcttctga tatcgtgacc gccgatgcgg atctgtccgt
     1201 ccgttgcatc ataaaaacgt gccagcagat tgaccagcgt ggattttccg cttccggttc
     1261 cgccgatgat acccacggtc tgtccttttt tcaccgtaaa atgaatattg gagagcgttt
     1321 ctgcaccggc accgccgtaa gtgaacgata catgatcaaa tgccacttct tcatctgaag
     1381 tgcctgtttt tctgtttttt gcattgtttt ccggacagga aaactccatt tccggctcta
     1441 cgctcagaac agaagcaaca cgctctgcac acgccattgc ttttgtcacc tgaatgatca
     1501 ggtttgccag cttgaccagt tcgatcagaa tctggttcat ataattcaca agggctacca
     1561 catcgccctg ctgcatattt ccaagatgga tctgcactgc tcccgtattt aagatcacaa
     1621 caaccgcaag gttgatcatc acgtaagtca gcggattcat cagtgcggag atctttccga
     1681 ccagaagctg gctgcgtacc agaagtccgt tgttctcctc aaagcgatcc ttctcctgct
     1741 cctctcttcc gaaagcacgg atcacacgca caccggtcag attctccctg gtgaccccca
     1801 ggatcccgtc aaggctgccc tgtaccttcc ggtaaagtgg catcgtgatc aacataatgc
     1861 caaagacaac gatggaaagc aacgggatcg ttacaacaaa aataacagcc gccttcgggc
     1921 ttacgataaa tgccatgaca atgcttccga atacaataaa cggggatctc aggaacagtc
     1981 tcaggaagag attcagtccg ctctgcacct ggttgatatc actcgtcatt cgcgtgatca
     2041 gtgtggaagt acccagcttg tccatctccg caaatccaag ggactggata tgtgagaaca
     2101 gcacatgccg caccttcgta cagaatccaa cggatgcttt cgccgcaaaa tactgtgcgg
     2161 tgatactgca ggtcagaccg atcactccaa gcaggatcag aagtccgcag tgtgacagaa
     2221 tatacccttt gtctttgttt gcaattcctg tgttgatgat cgcagccata acaagaggaa
     2281 ccagaaggtc aaacgttgcc tccagcattt tgaacgccgg ggcaagaatg ctctcttttt
     2341 tatagtcgcg aagatatata agaagttttt tcaaaatttc tcctttccgg tctcactgca
     2401 atttgaaaga aaaattctct tatataccaa aattgtaaaa tcagaacgct tttggcatcc
     2461 tatatatctt atccattttt tacatatttt acaagtaaaa aaaccgcccc ggcagttttt
     2521 cactgccggg gacgattttc cgatggttat aagttccgat cgggctgtct ttatatccgc
     2581 ttttctatgt gcttattttg tgatcttaac gcctgctttc tgtccttttc ctttcagcag
     2641 tcttttgtaa gatctcagct tcttagccgg tactttgatc ttcgctttcg catggatacc
     2701 cttgaatgct tttttgccga ctttcttcag agaagtggat ttgatcgtga tgctcttcag
     2761 gtttgttgct ttttcaaacg cactcttgcc gatggtctga acgtttttgc cgatcacaac
     2821 ttttttcagt ttcttatcgc ccttgaatgc tttgtttgcg attgcggtta ccttgaaggt
     2881 gtatttgccg ttcgcactct taacggttgc cggaacagta aaggctgtct tcttgttatt
     2941 tctcttaaca agtgttaccg taccaccctt tgctgcagat tttgtcacct tgtaagtagc
     3001 gtttttgtac ttgatcgtag caccttttgc cggaacatcg ttggccggag tcgggttggg
     3061 tgtaggggtt ggggtcgggg ttg
//
LOCUS       DAWDWH010000230         3064 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_11217_length_3064_cov_10.903955, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000230 DAWDWH010000000
VERSION     DAWDWH010000230.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11217_length_3064_cov_10.903955"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1302..1311
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          769 a          719 c          668 g          898 t
ORIGIN      
        1 atcatttcta tgaccgcaat ctgctggatc ttctctcaga gaagaccggt atcagtgaac
       61 aggggctgga aagtgcggac gagaaactgc cgcagaagat tctggatcac tatgttccga
      121 ccagagtaac ggactataat accagccact atctgttccg catggaaagt aaactgatcg
      181 aggagcttgc agaaaaggaa tgctgtgttt ttatcggaag gcttgcagac tggattttaa
      241 aagaccgcga cgatgtgctg aacgtattta ttacggcacc ggatgaagac cgcatccagc
      301 gtatcatgga aacagaagat gttacgcaga ctcaggcaga gaaactaatg gtgcagattg
      361 ataaaatgcg gaacaattat tattcttatt ttacagaccg caaatggcag catacgaaag
      421 accgtgacat catcgtcaac agttcgcttc ttggcattga aggaacggta ggtatgctgg
      481 agtatattgt aaatgcatca aattaagttc gaataagaaa ggttgtcgaa tgtgcaaatg
      541 aaactgcatt gcaaaatata gtgcgtaaaa taatacagcc atgatgaact cgaaaacaaa
      601 tcgctctgac ggtagcaagg cttccgtcgt gagattgttt tcgaatcatc atggcgtcct
      661 atttttataa aattattttt atggcgttcc tttgcacatt aagaaaatct ccgccacatc
      721 tttattttgc atccttcggc acatctttga tgctgaagct gatgcgtccc atcttgtctt
      781 ttccaaggca tacaactttc acaacatcgc ccagtgtcag aacgtcttct acatggttga
      841 tacgctcttt tgcgatcttg gagatgtgta ccataccttc ttttccagga gcgaactcaa
      901 ggaaagcacc gaactctttg atgctgatga ctttaccggt gtagatctgt ccctgctcgt
      961 aatcggttac gatgatgttg atcagatcaa cggctttctg catcatggca gcgtcggttc
     1021 cgcaaatgga gacagcacct tcatcggaaa tatcaatctt cacaccggtc tcttcgatga
     1081 tcgcattgat cgtttttcct ctctgtccta caacatcacc gatcttttcc ggatcgatct
     1141 tgatctggat gatcttcggt gcgtactcgc caacactctt acgcggctct gcaatggctt
     1201 ttgccatgac ttcatccatg atgtacattc ttgcttcgcg tgttcttgcg atagcttctt
     1261 cgatgatcgg tctagtaagt ccgtggatct tgatatccat cnnnnnnnnn nccatcaggg
     1321 acatgctgga tgcacagata cttgcctgtg aagtagaacc gttggattca aaagtctcgg
     1381 atacggtacg gatcgcatac gggaattcct cttcgcttgg aagaaccgga acgagtgctc
     1441 tctctgccag tgctccgtga ccgatttcac gtcttccagg tcctctggaa ggctttgtct
     1501 cccctacgga gtagcttggg aagttataat gatgcatata acgtttggaa atgacgtttt
     1561 catccagccc atccagtctc tgagcttcgg aaagcggagc cagtgtgcag acgttgcaga
     1621 tctgtgtctg acctctggtg aacatggcag aaccatgtac tcttgggatc aaatctactt
     1681 ctgctgccag cggacggatc tgtgtgatcg cacggccatc cggacgtttg tggtctttta
     1741 agatcatctt acgaacagtt tttttctgat actgataaac agcttcatcc agaacagcaa
     1801 gccattcttc attctcggca aaagcttctt ccagtttatc tttgatctca cggatgtttg
     1861 cttctcttgt ctgtttatca tcggtaaaca cagcttcttc catctgttcc ggtgttacga
     1921 tctctttgat ggctgcaaac agttcttccg gtacagcaca gctttcatag ctgtgtttct
     1981 cttttccaca ctcggcaacg atcttgtcaa tgaatgcaat cacctgctga ttgacttcat
     2041 gtgccgcaaa aattgcttcg atcatcttat cttccggtac ttcatttgca ccggcttcga
     2101 tcatgatgac tttttctctg gtagatgcaa ccgtcagagc catatcagaa acctgacgct
     2161 gtgcggaggt cggattgaat acgaacttac cgtcgatcag acctacctgt gtggtagcag
     2221 tcggtccgtc aaacgggata tcagaaatag cagttgcgat cgcagaacca agcattgcag
     2281 tcagacataa ccatgttgtt cagagttaca tcgttgcggt aatctttcgg gaaaagcgga
     2341 cgcatcggac gatcaatgac acgggatgtc agaatcgcat tttcagaagc ctttccttca
     2401 cgtttgttga agccgcccgg aatctttcca acagcataca ttttctcttc gtattccaca
     2461 ctcagaggga agaaatcaat cccttcacga ggcttttcgg atgcagtagc ggttgataat
     2521 acggtagtat cgccgtaatg cataaatgcc gcaccatttg cctgtgctgc cactcttccg
     2581 atatcaacgg tcagagttct tccggcaagt tccatagaaa aacttttgta cataattact
     2641 ctccttttct ttttcgctga atttgttttg cttttacggc agcctctccg aaacaactga
     2701 aaaacatttt atcatcaaaa tatatcgtta aaatactttt cagtggtctc ggaatgtaaa
     2761 gcagctgccg gctcggtttc tgttaggaga aaccgttctg gaagtttacg catacttcaa
     2821 gcaacgtaaa cagggggacg gaaatcgttc cgtccccctg tgattcccag attattttct
     2881 gataccaagt tttgcaatca gtgcacggta tccttccaga tctgttttct taaggtaagc
     2941 aagcagacca cgtctctgtc caaccatttt cagcagacca cgtctggagt gatgatcctt
     3001 cggatgattt ttcagatgct ctgtcagctc ctggatacgt gctgtcagga tagcgatctg
     3061 tact
//
LOCUS       DAWDWH010000231         3046 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_11296_length_3046_cov_13.855901, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000231 DAWDWH010000000
VERSION     DAWDWH010000231.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11296_length_3046_cov_13.855901"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          715 a          805 c          535 g          991 t
ORIGIN      
        1 tatgccttac ctctttcttt tgcactttgg tgtgcctctc tgtttcatga ttgtattata
       61 caatcatatt cgttcaattt caatcatact aattcacaaa ttttcctttc tgtttttgta
      121 tagttttcac aaacgttcat tttcattcat attcgttcaa ttttcattca aaatttgcca
      181 tgatgtcagg gtcaaatttg ttgctcgcca tttatagaag tgggagtctt ctccgactga
      241 gataaaaaaa accacgcacc accgaccgat tcagtcagca agtgctgtgg tttcttttct
      301 ttgcattttc agatcagtcc tcgtttgccc gcttccatca cggctcctgc cttattggaa
      361 acaccgagtc ttttgtagat ctgctgtgta tgatatttta cattagcggt tgtcatattc
      421 agctctgccg caattttttc ttttgacatt ccatctgcct gcagtttcag gattcgaagc
      481 attgtatcac tcaatgtgac ttcttccaca cccactttta aatacgccgg atagaatcgt
      541 gccatttttt ctgtctcgtc ctgaaccttt gcccagaact gtttgttctt tctggtctgt
      601 gccagatctt tttctacttc cgacggcttc caggatgttg cctgaagcag aggccggata
      661 gccgcacctt ctcttgtgat gatccgtaca aaatgatatt cttctgcttt tgtcagtgcc
      721 tcgccaagtg tttcatccca ttttttctca cccatgcggt actgcgtgat cgcaagaagc
      781 agtttcactt ctatatcaat gtaggtacgc tgcatcactt ttgcataata ggcacatttc
      841 aaaagcagat gataagcctg ctcgtttctg ccattctgca gatacacacg cactttcgta
      901 atataatgaa aacgatcata aatagagaat atcagttctt catccggtgc ctgggcaagc
      961 cattttgcaa tcgctgcttt gtccgcatca tataatgcac agcgaacgag aaaggtatcg
     1021 atattcggca gaattctttc tgcattttcc tgctgtgctt tacccttgaa tctggtcaga
     1081 agctcctccg cttcttttac ttttccggta ataatatgaa gccaggcaag catgccgtct
     1141 ccgacaaaac actgctcgat cttgcctcct gcctccgcct gcattcttcc cttatttgca
     1201 agcatcgcaa cttcataatt atctccgccc ttttcaaaaa agctttctgc aagtgccaga
     1261 tttacaagcc ccttaccata ttttccaagt acaaattcca caatcttgcc aatgcttcct
     1321 gcaagctcgc ggtcacgctt gctccactca cagaaatcct tgccgccgtt catctgagat
     1381 gcctgattgc tggtaacgga aaactcctgc attttcactt ctttatccaa aagaagcaaa
     1441 tatgcatttt tcaggatttc taccatatgg tcactgccac ggtgcggaag tccgatatcc
     1501 agatacagga tctttccttt cgctgaacgc cgttcacttc cggtatgttc ttttgcatat
     1561 ttttgaagct cccggtacca gcgttcgctc tcttcggtat ttaaaagcaa cgactgcagc
     1621 atactcatgc cacacatcag ttccgggctt ttgcatattt tttcttccgg cagtttaaga
     1681 taatacggtt tcagttcata ataataagca ttgtttggtg ctatccgaac attatcgatc
     1741 agcaccgagg cgattctttc cgtgtcattc acctgttgat acatggaaag agcctttaac
     1801 ggctgcttac tgatctggta ataaagtcct gcattttcgt aaagcttacg gatctgctct
     1861 tttgtgtatt ttcgcctcag cctccggatc atgctgatcc gcatttgatt tctgagtttg
     1921 tagaccgttt cacttccgtt tcggacaata tccatgaaat taccgatcca ctgaatcctt
     1981 tcaagaagcg attctacatc attgcgtccg gtgatcatct ccgccatata tacggtaaaa
     2041 tcttccacaa ttgcaacttc catcagaaat tcctgtattg atatttccca gcggtcatag
     2101 acatgatatt ccagataatc ccacatctgg ctcagtgcct ctcccaccat cgtccggtat
     2161 tcttcatctg aaaacggttt cgtgacatcc ttttcatcct gccccatacg catctgccaa
     2221 tagacactat ttgtgatgct ccagccgatc ggcataccaa cgcagtatgc tttttctttt
     2281 gcccgctgtt cttcactgaa gatcatcccg gtcatggcaa tatactgatc tgcctgatct
     2341 tcatcaaaca gcagctcctg ctcaccgatc acatagaaga cttcacgata ccgcacagca
     2401 gtcagccacg gcggtaccgc acagcgtccg ctcaaaatca gccacacatc ctcacgctcc
     2461 acaagttcga tgatcgcttc ccggatctcc tcagtatcat tctgcatcgc catatcgtgc
     2521 agactgttga tcaccacggt tttccgctgc ttggaaacct tgatctcacg cagttcctcc
     2581 actgttacca aaccagcgtt aaacagcgta tatttccggt tcttcagata tcgcatgacc
     2641 agttccgttt tgccgcaacc gctgacccca taaatatacg ctgttatatt ctcttcatct
     2701 gccgctttca gccgcctgat tgcacggcgg ggttttacat agcagatttt ctccccttgc
     2761 atcatctctt cccacatgct gttttctgcc aatgcaacac cccgctttct ctgttgtttt
     2821 actacgtttt atgtttataa gttcctcttc ccaagaaaaa gtatagcaat ttttccgtgt
     2881 tttgtacact atccaaacgg ctagtttttt tctgaaaagc aaggaaaatt tgcaggattg
     2941 tgagagttac cttgtaatgg agcaattcgc aggcatccat tgaagtcaaa agttttttat
     3001 ctcagtcgag aagactccca cttctgcaag tggtgagtaa taacaa
//
LOCUS       DAWDWH010000232         3019 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_11404_length_3019_cov_10.054656, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000232 DAWDWH010000000
VERSION     DAWDWH010000232.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11404_length_3019_cov_10.054656"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    311..389
                     /estimated_length=79
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          940 a          621 c          736 g          643 t
ORIGIN      
        1 gaatggatgt accgctaatt tcagaatagg cgtaccgttc gagtagaatt tgcagtccat
       61 gccattttgg atcatcagca cgatcgtcat attttatatc tgcaggctat atcatagtat
      121 atggagactg gcaagcgtag cagagcttca gatgatcctg attgcataca gcatactggc
      181 agtggtttat tcactgggca tgctgttcat gcagatgcat atgccaagat cctactattt
      241 tatcggattt ctgttaaact ttctgatgac aaccggactg cgtttttcct atcgtctgat
      301 gcggttctgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna aataccaatg cagatacagg cggtgaaatg
      421 catgcaaaag accatgtgat ggtcatcggt gccggagcag caggtcaggc actgatcaaa
      481 gaaatcatca attcaaaaaa actgaataca caggtagtct gcattatcga tgacaaccca
      541 accaagcatg gacgtgttct ggaggggatc accattgtcg gtgaccgcta ttccattcca
      601 gaaatggcaa agaaatataa agttgaccgc attatttatg caatcccgga agcagcggca
      661 gatgaccgca aggccatctt aaatatctgc aaggataccg gatgccgtct acagactgta
      721 ccgggcgttt accagctggt aaacggagaa gtcagtgtca gcagacttcg caatgtcgag
      781 atcacagacc tgcttggacg tgctcaggtg caggtaaaca tggatgaaat ctattcctct
      841 attcagggaa aaaccttgct ggtgacagga ggcggaggct ccatcggaag cgagctctgc
      901 agacagatcg cagcatctca tccaaagaaa ctgatcatct ttgacatgta tgagaacaat
      961 gcctacgaaa tccagcagga gcttcgcaga aaatacggcg acagcctgga tctggaagtt
     1021 ctgatcggat cggtccgcaa tacgaaccgc attcgttcca tcatgcagaa atacaaacca
     1081 gatgtcgtat ttcatgcggc agcacacaaa catgtgccgc tgatggaaga cagtccgaac
     1141 gaagcaatca aaaataatgt tatgggtaca tacaagacag ctgtggcagc atccagggca
     1201 ggagttaaga aatttgttct gatttccaca gacaaagccg tcaatccgac caacatcatg
     1261 ggagcttcca aacgcctctg tgagatggtc gtgcagatga tgaaccgcca gtgcaaagac
     1321 accgacttcg tggcagtccg cttcggaaac gtgcttggca gcaacggaag cgtgatcccg
     1381 ctctttaaga aacagatcgc agagggcggc ccggtgaccg tcaccgataa aaacatcatc
     1441 cgctacttca tgaccatccc ggaagccgtg tcactggtcc ttcaggcagc acattatgca
     1501 aaaggcggcg agatcttcat tctggacatg ggcgaaccgg tccggatcga tgagatggca
     1561 agaaacctga ttcgcctgtc cggattcaca ccggatgtag acatcaaagt cgagtacacc
     1621 ggcctgcgtc cgggagaaaa acttttcgaa gagctcctga tggcagaaga aggcatgcag
     1681 gagacaccaa acaaactgat tcatatcggc aaaccaatcg agatggatga cgtagcattt
     1741 aaaaagaaac tcagacagct ggatgaggcg gcataccagg agacagaccg gatgaaaaaa
     1801 cttgtggaag aagtcgtgcc gacgtatcat ccggaaaacg caggaaatgc atgatttagt
     1861 catactaaga caacagagaa aagtaatatt aagataaaag aggcataaat taatgaaaaa
     1921 atttgaaaaa aaagtctggc tcagttcacc gacgatgcat ggcccggaga ttgagtacgt
     1981 aaaagaagct tatgaaacaa actggatgtc cacagtaggt gccaatatta atgaagttga
     2041 gcgactggcg tgtgaaaaaa caggctgtgc ttatgccgta gcactttcag cgggaacagc
     2101 agcattacat atggcggtaa aacttgcagg agtaaagcca ggagacaaag tattctgttc
     2161 tgacatgacc tttgatgcaa ccgtaaaccc ggttgtatat gaaggcggta ttccgatttt
     2221 tattgataca gagtacgata cttggaatat ggatccggtg gcactggaaa aagcgtttga
     2281 aatgtatcca gaagtaaaag tggtagtgac cgcacatctt tacggaacac cgggaaaagt
     2341 tgatgaaatc gaagatatct gcaaaaaaca tggagctgtt atcgtggaag atgcagcaga
     2401 atcattaggt gcaacttaca aaggagtaca gacaggtacg tttggaaact acaactgcat
     2461 ttcctttaat ggaaacaaga tcattaccgg atcagcagga ggtatgctgc tgacagacga
     2521 caaagatgcg gcagaaaagg tacgcaaatg gtcaacacag tcacgcgaaa atgctccgtg
     2581 gtatcagcat gaagagttgg gatataacta ccgcatgagc aatgtaatcg caggagtggt
     2641 acgtggtcag ttcccttatc tggaagagca tattgcacag aaaaaagcaa tctacgaacg
     2701 ctacagggaa ggatttaaag atttgccggt tcaaatgaat ccatacgatg aaaagaacag
     2761 tgaaccgaac ttctggctca gctgtatgat cattgatccg gaagctatgt gcaaacaggt
     2821 aagaggagaa tgcgaggcat tatatgtaag tgaaccaggc aagagctgcc cgacagaaat
     2881 cctggaagca attgcagcaa tcaatgcaga aggacgtccg atctggaaac cgatgcatat
     2941 gcagccaatt tatcgcatga atgcatttgt taccagagaa ggcaacggaa gagcaaaaac
     3001 aaatgcatat atcagcggt
//
LOCUS       DAWDWH010000233         3013 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_11436_length_3013_cov_7.131508, whole genome shotgun sequence.
ACCESSION   DAWDWH010000233 DAWDWH010000000
VERSION     DAWDWH010000233.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11436_length_3013_cov_7.131508"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2236..2245
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          727 a          825 c          589 g          862 t
ORIGIN      
        1 tcccccactc ttcgtccccg cgtccttttc ttgctttgtt gtccagatac agccggaaac
       61 atttttccgt ctttgcaagc accggttttg tctttcgctc cagccagaaa tgccatgccg
      121 tgatgcagtc tctttcgtat tccagtaatg cgacatgata cgtcagaagc tcttttttct
      181 ggttcatcag ataggtataa aagctctcca gatgtccctg caaaaaaatg gctgaggacg
      241 gtatgccaag ctttaaaagt gccgtgcgga tcacatctgc ccagatccct ttcatctcgt
      301 gcatcgtcac cataaccgtc atatgttcca cagaccctgc accttttaac aatgcaaaac
      361 attctttcaa aaactgatac agtacttcca cgcctctggc atctttccgt tcgaccaaag
      421 agaacagatc gaccggtgtc ctgatcaggt aatcgttttc ttctttttcc tcctctgact
      481 gcatgaccat cagcggtgca cggtcagatt tactgcaaaa tgcaatctga gaataatccc
      541 gtccgagatc aattcccact gaaatattgc catgttctgc caacatactt gtcgttcctt
      601 tctctgtccg cttttatctg accggcagaa aatgctctgc cagactttct tttttcagat
      661 attcttctgc ctgtgcctta aactccgttt cctttttctg tacaaatgct ctgcacatct
      721 catttaacat accgtaacgg ctctgcatgt cgttatcttc cggcatttct gtccttggca
      781 gcagtgtaaa cggaccggac tgcgttttct tcccgtcctt ttcctcatga atcatgcaga
      841 ccattttttc accgtaaaac agggtaaaat ctttctcaaa aataccttcg tatacattat
      901 gcagcatttc cgtatgctcc acggaagtgc cttcttcctg attttccagc gtgtacgtca
      961 gcatcacgat tgcctttgga tcggtgcggt actcgatgat gctgcggtcc ataagctgaa
     1021 gggtacgcat cagagacacc ggcagctttt caaagaatcc aaagcacata tgcttatcgc
     1081 cgcactcctg aaggatctgt gacatcagga tcagctgctt ttcggaagct tccccacatt
     1141 cggtacggta cctgagataa gccagtctgc atgccggatc aacggattcg gatgcattca
     1201 tccttcgctc aatctcagca aaaaccggct tctgcacgtc catccctttc acaaaatact
     1261 ggtaagacat cagattcaga taagccgcaa gcaacatctt cttgccatac ttccggcggt
     1321 aggattcaaa gatcggttcc gtttcttcca tgcccgtccg ctcaaatgcc agcatgatca
     1381 ggattttttc ttccagtgcc atggtatcca ggtcaaaatt cttgccggct ttccacaggg
     1441 tcttcatcgt ctccatcggt ccgtcgtaac ctgagagcag ataagaaaga atggcctcat
     1501 cgtattttcc gtggcagaaa cagtaatggc acagatagag cagcatttcg tcctcttccg
     1561 gtccctgctc caagatcctg cggctgcaca gacgcaccag aacgccaagc gggatctctt
     1621 ccggtccgaa cctggatacc agaaggaatg cttctttgca catgccttct ctgaccagaa
     1681 gttccaccgt ctttgcctta tccaccgccg caaaaacctc aagatccata tcgtgcaggt
     1741 gctcatagag cgtatcatcc tgcagattat cataataata ctccaggatt tcttttctta
     1801 gctgctgctt gtagtcttct tttacatcca cgaactccag catgccgaaa aagtctcgga
     1861 tcttctcctg tgtgatatcc cttgatctct gtgcttctgt acaggaatac agcagaaatc
     1921 ccggactgtc cggtgcgatc ctgcggcaca tagaaaacat atcactgccc ttaagcagtc
     1981 tggtaatggt ataatcaaat ccttttgtat gccatcttcc ttcgcggtcg atgataaaaa
     2041 tacaggcatc ttccgtatag atctgcacgt ttgccgtatg tttggagaag atcaccgtct
     2101 ggatctcttc gagctgtctg tggacaacgg caactgcgga tgcatttgga tctttacagc
     2161 agatctcaca ggtgaaaagt gcatgggaaa gattctccgt catccttgta tttaacatgg
     2221 attttgtgag aaactnnnnn nnnnnttctc ataggaaaaa tacatccgca ccatctgcgg
     2281 caggagtcct gtccgttctt ttcccatgga ctccacataa tattcataca atccggtcag
     2341 acgcagtcct tcggcaacac ccttttcata ccagttaaaa taagcagcac ccactttatg
     2401 tcctttgatc agcagactgc agatcgcact gaccacactc tttgacggat acttggtata
     2461 gcactggcac aggatctgat acatggtcgc atcataacgt ctctgccgtc cggcagtatc
     2521 tgccacctgc atcacgagtt ctttatccag aagatcgttg cggcagataa aacgcagcac
     2581 ctgaatctca aaatcatcca gcttctttaa cagaagcggt gacttccgaa acagcgtgaa
     2641 cgcctccaga tacatcagtc tgctgcggca gcccatttta tactgtcttc ggattgcttc
     2701 cagtttctgt gacggatgct gcagaagatt ctccttcaga tataacagca cccactgaag
     2761 cagccattct tcctgatttt tcaggtacag atcctggatc ttgctttcga tataatcaat
     2821 atatttctca tctttatcat aaagagtcgt cagatactga taatagccct gcacggccgg
     2881 acggtctgag atcctccgtt tctgctccag cttttcaagc aaaggacagg cgatctccgg
     2941 ctgatctgcc gcaaaataga tctgtacctg atacagcttc atcatgatat cctctccgcc
     3001 tgcctgttca tag
//
LOCUS       DAWDWH010000234         3002 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_11481_length_3002_cov_7.085511, whole genome shotgun sequence.
ACCESSION   DAWDWH010000234 DAWDWH010000000
VERSION     DAWDWH010000234.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11481_length_3002_cov_7.085511"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          884 a          730 c          582 g          806 t
ORIGIN      
        1 cgcctctggt actggctgct cacgttgata ctttaggtgc tatggttcgt tccatcaagg
       61 aaaatggacg ccttcgtcct acaaccctcg gtggtcatca gtggtctacc gctgacggag
      121 aaaactgcac tatccatacc cgggacggca gagtttacac cggcgtggtg ttaaacaccg
      181 aaccatctgc ccatgttgca gatcaaaaag ttgaaactct ggaagagaac atggaaattc
      241 tgctggatga aaatgtatcc tccaaagaag atgttctgac cctcggcatt cagaccggag
      301 acatcatcgc catggatccc cgcacagtta ttaccaaaag tggatacatc aaaagccgtt
      361 ttctggatga caagctctct gctgctatcc ttctcggcct tgccagggct atccgggatg
      421 aaaactggaa actgaaccgt aaagtttctt tgttgtttac agtctatgag gaagtgggac
      481 acggcggatg tttcatacct tcggataccg aagaaatgct ttccgtagat atgggctgtg
      541 tcggtgccga tcttggctgt acagaacgga tggtatctat ctgtgccaaa gattccggcg
      601 gcccttataa ttatgacctg gtaaccacac tttctaatat tgccagagag cagaagctgg
      661 attatgccat tgacatttat ccacattatg gatctgatgt agaggcttct ttacgtgccg
      721 gctacgatat ccgtcacggc ctgatcggac ccggtgtcta tgcttcccac aactatgaac
      781 gttcccatgt aaatggagtt cgctgcacct ttgaactgtt aaagacctat attacgaaat
      841 aacaaacagc cggatacttt tgatcatgtt tacagaatca gaagtattcg gctgttttta
      901 tctgagcaga taagagtcat tatcttctca gaaaacttat ccaattatta tatccagtcg
      961 gcaaatccgg cagataccag gaacagaatc aacatgacga tcggcactac atagcgaatc
     1021 ataaatcgat ataaaccggc ccgcctgaaa tgttccccgt ttcgctccac ctctcctatg
     1081 atccagtcgg gtccgatcac ccagccgatc aggatactgg tcagaagtga gataaatggc
     1141 atcaaaaaac tgttgctgat ataatccata acatccagga gctgtccgac agaaccattt
     1201 ggaagtttca gttcgaaata cagtttatta tacccaaggc agatcagcac tgccgtaata
     1261 agagaatata ctccgaccac gccgcacatt tttttacgtg gtttatgaga aagctccatg
     1321 cagtttgcaa ccagagtttc cataaccgat acacaggagg tcagagccgc aaatcccatc
     1381 atcaggaaaa acaggattgc caccggacgg ccgaagattc ccatggcttc aaataccttt
     1441 ggcaaagaga taaagatcag gcttggcccg gaagccattc catcgtttcc aaggaacaca
     1501 aagacagccg gaatgatcat cattcctgca agaaatgcaa ctccggtatc aaaaatttca
     1561 atctgatttg tcgccttatt caaattcact tcattcttta cataagaacc ataggtgatc
     1621 ataattccca tcgatacact taaagaaaag aacagctggc tcatcgcatc cagaagaatc
     1681 tcaaggaaac gctttactgt cagaccgcta aaatccggtc tgatataaac agccagtccc
     1741 tgaagaccgg tacgaacagt accatctgca tccgtatgag acagggtcag tgaaaaaaca
     1801 gcaataacca gaatcagaag gatcagcccc ggcatgataa ttctggaaaa cttttcaatt
     1861 cccttttcca caccacaaaa tacgatccag gcggtcagtg ccagaaagat cagcataaat
     1921 acgatcggtg caatatccga tgtgataaac gatgtaaaat atccatctgc tgccgcttcc
     1981 tttccatcag acacaacata aataaaaaag tactttgcga tccatccacc gatgacagaa
     2041 tagtatgtca tgatcagtgc cggaaccaga aaggtcaaat gtcccaaaaa tttccacttt
     2101 ggattaagtg cttcaaaagc tctcaatgca tttttctttg tttttcgtcc gattgcaaca
     2161 tctgttgtca gaagtacaaa accaaaagta agtaccagca ccagataaac cagaagaaac
     2221 agaccacctc cgtcttttgc acagagatac gggaatctcc agatattccc gacgcctacg
     2281 gcacttccgg ctgctgccag gacaaatcct atggagccgg aaaagctgct tcctttttct
     2341 ttcatttcaa tttcccctct tcaaataaag tttgataaag gctgaatctc ttcagcctaa
     2401 tatttctata ttaagccaaa taagaagatg tgtaaacggt aaaattttct tttcacgaaa
     2461 ttttactcct ttgagccgtg catactccgg atatagaaag caatctgata gggcattctc
     2521 tcttccggtg tcttcagttc atttcccaga atttttctga tattgccaat tcggtataac
     2581 actgtgttac gatgagtata caattctgca gccactgcct ggatacttcc attatgtttc
     2641 agataggatt ttagcgtttc cacgtaatcc gcgtggtatc tgcgatcata ttcttccagt
     2701 gcttcaagac attctctttc tatttcttcc agaatcttct tgtcttctac cctgtaaagc
     2761 aggcgaaaca acccgcagtt atcatttgtg ctgccgcttt tgccctctga taacttacag
     2821 agagctgtga aatatctata catctgctcc cgatcccgac acacaattcc aatcctggca
     2881 tacgcctttt tcctcttttt aaggcttttt ctacaagatt acacaaatac tcttccgaaa
     2941 gtgcattggc taccagcaca aaatccgaat tataataaaa gaaactggca ttgtgcgaaa
     3001 tg
//
LOCUS       DAWDWH010000235         2961 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_11645_length_2961_cov_10.631452, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000235 DAWDWH010000000
VERSION     DAWDWH010000235.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11645_length_2961_cov_10.631452"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          864 a          572 c          794 g          731 t
ORIGIN      
        1 atcgtgaaaa caatcaagca gtgcaatgtc ttcgttggga agaggacttg ttgcggatgg
       61 ttttcttaca tcagagcgtc cgatcagata atcggtgctg acatggaaaa tatctgccat
      121 tttgatcaga gatttcagac ttggcagatg ctttccgttt tcgtacgcac tgacggtttc
      181 ctgagatact tccagctcca gactgaggcg aagttgtgtg atattctttt cggcacgtaa
      241 ttcacgaatg cgatttttca tttgtaacct ctccttctcc aatatcatac aatttatact
      301 tgtatcgctc agaggcagct gttataataa aaacaagtat aacctgtaaa atatatgggg
      361 agaacaggta aagatgccaa aacttcgaaa atcagtcgaa agacagaaga gaagcgggag
      421 aaacgttcag aacgtctgca atcgtaaaca gcttttcaag ggagatcgaa gtgatctttc
      481 ccggagcttc cagattgctc agatgtgtcc ggctcatcga tatcagggcg gcaagttcct
      541 cctgtgtgag tccgcgcagt ttgcggtaac gggcgatatt tagcccaagc tgctgtagtt
      601 gtctgggata atttgtgttt gacatgagtt gatacctccg ccaaaacttt atatgggaaa
      661 aatgagaata aaaaggctca gcaggttcat tttgcaaagc cacatattgc aaaacaagag
      721 aaaagatatt ataataacaa aagattataa aagaaatggc tcagtgtttt tccatctgtt
      781 ttctgtaaca ggcataagat aagacttcgt tcagtgccgg ggaggccata tcctggcagg
      841 ttgcaaagag gagatacatt tcggcactgg caaagggata tttctccgga cagtcggtca
      901 tgccgaccag atagtcaagt gaggtatcat aaaattctgc cagaagcttc agtatatcca
      961 gaggtggcac acgcaggccg ttttcgtagt tgctgtagga agaacggctg agatggattt
     1021 ttgagcagac ataggactgt gtgtagtttg cttttgcacg gagaaatttc agacggtttg
     1081 caagatcgtt tgtgttcatg aagaacctcc catatagtat atttgttgtt ggtgatgcag
     1141 tgaaaaatgc cattaagcca taaaacggct cctgtgcaga catatcgtgg ttttggacgt
     1201 ttgcccctga catcttcaca gttgagtata acataaaagc cttgggtttg gggaaaataa
     1261 cagagtttga ggaagagaag tgggaattga cagaatgaaa tggaaatatt tgcgttatgt
     1321 aatgaaaaac aaaaacagta aaaatccgtg gaaacggatg attgtaagat ggtacacgat
     1381 gttcttaagg cgaggatgcc gcagagaacg gcggtatctg gcaaaaaggt atattcgcgt
     1441 cgcaccgccg aaatacacgg caaaagatat tctgcaaaaa gcaggaaagt aaacagattc
     1501 agcggcttaa atagctgttg tggtaacgcc cgtcaaatgt gacatcttcc ccgaaccagg
     1561 cgggcggaca gaaagaattg gcacttgctt cgtccgggaa ttcgacttcg gcaagaagca
     1621 ggcctttgca gggttcataa aatacatcca gttcgatggt aagaccattc tccataggga
     1681 tcagataacg tctcttggag atcagtttgc cgtcgatttt ttctttcaga tgggcataac
     1741 cttctgcacc gagcggcagg ttgtattctt cccgtgccat caggccgccg gatttgtagg
     1801 tgaggatata gtcgtcgtcc tgacggcgga tgcggacgac cggtttgcgg cagagatagc
     1861 cctgctcgat cagaacactg cggcaggatt caagatccgc aggaagattt ttttcatcga
     1921 tcagatattt tcgttcaatt tccataaatc agtacctcct gaaaaaagaa tatcgcagaa
     1981 aacaaaaaag tgcaagtaca agtagattgg gactggaaag aagcgtctgt ttttggtaga
     2041 atgtcagata gatcaacagg aaataacagg gtatgcagat accggaacgg aggataaaat
     2101 gagtagagtt gggatttttc ttgcagatgg atttgaagag atcgaagggc tgacggtggt
     2161 tgacatctta agaagagccg ggatcgatat ttccatgatc agcatcaatg ggaaaaagaa
     2221 agtgacggga gcacatggga tcgctttaga tacggacgaa gatatcgtac agtgcgatcc
     2281 ggataaactg gatatgctgg tgctgccggg cggtatgccg ggcacgacga atcttgcggc
     2341 atgtgaaaag ctgacggagg cactgaaaaa ggcagaccag gaaaaacgcg ggattgcggc
     2401 gatctgtgcg gcaccgagcg tgcttggtga cctcggattt ctgaaaggga aaaaagccgt
     2461 ctgctatccg ggatttgaaa gccgtctgac cggagccgaa gtgcttgcag ttccggtcgt
     2521 aacagacggg cacattacca cgagccgcgg catgggaacg gcgatcgcat ttgcactgga
     2581 actgacgaaa agactgaagg atgaagagac ggcgaagcag gttggccgtt cgatcattta
     2641 taacgtatga tatggacgat acgtgatctt aagaaaaaag cacggaaact tctgaaaaac
     2701 tgttactggc cggcggttgc agtctgtctt ctgacgacga tgctgaccgg atggatcggc
     2761 agtgcgggca tcggtatcgg caggatccag gcagaaaatg catccgggct tgagatgtca
     2821 gagcagcgga tgcgagaact gaaagatgat ctgaaagacc gcaatccgat cgcagaatat
     2881 aagatcagca ttgtacagaa acagctgaat ctgctggtct ttgtctttgc ggtcacagca
     2941 tcgatcgtga tcgtggtgct t
//
LOCUS       DAWDWH010000236         2910 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_11842_length_2910_cov_10.412609, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000236 DAWDWH010000000
VERSION     DAWDWH010000236.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11842_length_2910_cov_10.412609"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          917 a          555 c          769 g          669 t
ORIGIN      
        1 ttcttgttag atatggaaaa aacatgataa aataattaga aagggggcga ttgccatgac
       61 gattcgagag atggcagagc aaagagaagt ggcttatttc agcccgtatg catccctcag
      121 cagcaagtcc aggggacgcg accgggaaga gccgccatgc gatatccgtc cggtctatca
      181 aagagaccgg gatcgtattt tacattctaa agcatttcgc agactgaagc ataagacaca
      241 ggtttttctg ctgccgcagg gagatcacta ccgcaccagg ctgacccaca cgcttgaggt
      301 agcacaaacg gcgaggacga tcgcaagagc cctgcggatg aatgaagatc tggcggaggc
      361 gattgcactt gggcatgacc ttgggcatac accgttcggc catgcaggcg agcaggcgtt
      421 aaatgaaatc tgcccgtttg gttttgctca ttttgaacag agtgtccgcg ttgtagaact
      481 tcttgagaag aacggaaaag gtctgaatct gacctgggaa gtccgtgacg ggatccgaaa
      541 tcacagaacg tccggtcatc cttccacgct ggaaggaaag atcgtacggc tttcagataa
      601 aatcgcatac atcaatcacg atattgatga tgcattgcgt gccgggattt taaaagaagc
      661 agatgttccg gcagaatacc gggaagtttt gggtgatacg atccgggaac gtttaaatgc
      721 tctgatccac gatattgtga tccacagtca gggcaaagat gacattgtca tgtcaccgga
      781 gatggaaaaa gcgatgcggg gaatgcggaa ctttctgttt gagaatgtgt atcacaatcc
      841 gctggcaaaa agagaagaaa aaaaggtccg gcatctttta caggaacttt atcagtacta
      901 tatcgagcat cctctggaac tgtcagagga atatttaaac ctgatggatg cggggcagga
      961 gaaagagcgg gttgtctgtg attacatagc agggatgacc gatcagtatg ccatgcaggc
     1021 gtttgcagac ctttatattc cgaaggcatg gccggtaaaa tgaataagca ggaaataagg
     1081 agataggtat gtattatccg gaagaactgg tcgaagagat cagagagaga aacgatattg
     1141 tcgaagtgat cggcacttat gtcaagcttc agaaaaaagg aagctcttat tttggacttt
     1201 gtccatttca taatgaaaaa tccccatctt tttcggtaag tccggacaaa cagatgtact
     1261 actgtttcgg atgcggagca ggcggcaatg tatttacgtt cattatggaa tatgagaatt
     1321 acagttttcc ggaggcactg aaatacctgg cagaccgggt cgggatcaag cttccggagt
     1381 gggaatattc agaggaagaa aaaagacagc aggatctccg catgcaggtg ctggaaataa
     1441 acaagatggc agccaactat ttttattatc agctgcgtac ggagagtggg gctcaggcta
     1501 tgcagtacct gcaaagcaga cagctggatg atgagacgat caaacggttt gcacttggat
     1561 attccagcaa atacagcagc aatctatatc agtattttaa aagcaaaaaa atatccgatg
     1621 aattattaaa agaatccggg ctgtttaatg tggatgaaaa aaatggcatg tacgataaat
     1681 tctggaatcg tgtcatcttt ccgattctgg atgtcaacaa ccgtgtgatc ggtttcggag
     1741 gccgtgtgat gggagacggc aagcctaaat atttaaattc gccggagacg atcgttttcg
     1801 ataaaagtcg caatctgtac ggactgaaca ttgcaagagc agcaaggaaa aagtatctgc
     1861 tggtctgtga aggatatatg gatgtgatct ccatgcatca ggcgggattt tcaaatgcag
     1921 tggcatcgct tggaacggct ttgacggaga agcatgccac acttctgaaa cgttatacgg
     1981 acgaagtaat cctgacgtac gacagtgacg atgccggtgt gcgtgccgca ctgcgggcga
     2041 taccgatcgt aaaacgtgca ggactcaggg caagagtgct gcatatgaat ccttataaag
     2101 acccggatga atttatcaaa acccttgggg cagagaagtt tcaggaacgc attgatcagg
     2161 cggaaaacag ttttatgttt gaaatttccg tgttggagag aaattatgac atgaaagacc
     2221 cggaaggaaa aacttctttt ttccaggcgg tatcggaaaa actgatggag tttgagcagg
     2281 agctggaacg tgaaaattat attcaggcag tcgcagagaa atatcacatc ggatttgaca
     2341 atttaagaaa aatggtgaat cgcagtgcga tgcgtgcaat cggcagggaa gtaccgaaaa
     2401 gtactgtgga ccgagaacga aaagaaatcc ggaaaaaaag tcaggatgac ggcatgaaac
     2461 agtcacagag actgatgctg acctggctga ttgataaaaa aggattgtat gcaaaagtaa
     2521 aaccatacct ggatgcggat gatttcacag aagatcttta tcatcaggcg gcacagcttt
     2581 tgtttgaaca gcttgaaaag agcgagctga atccggcaag gatcatcaat cattttgaag
     2641 atccggaaac acagaaagaa gtcgcatcgt tattccatgc caagttaaac ctgcagtcgc
     2701 aggcggaatt tgacaaagca ttgaatgaaa cactgcgaaa gattatcaac aacagtattg
     2761 aatttcgaac gaaaaacatg aatccggcag atattgcggg attgcagaaa atcttactgg
     2821 acagaaagaa agttcaggat atgccgaaga tcacatatca gatgtgagat gaatcctgca
     2881 tccgcaggcg gcagcagcaa gacagcacag
//
LOCUS       DAWDWH010000237         2897 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_11895_length_2897_cov_7.273399, whole genome shotgun sequence.
ACCESSION   DAWDWH010000237 DAWDWH010000000
VERSION     DAWDWH010000237.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11895_length_2897_cov_7.273399"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          667 a          802 c          536 g          892 t
ORIGIN      
        1 cttctaccgt ggttgctgtc gtgtcaatat cccagccttt ttctttcaga gcctttacca
       61 cataagtgac ctgaggtgct gcaagtccga cgctttccag ctctttataa tgctgaaaga
      121 cctgttttgg tgtatcatca aacaaaatct ttccgtcatt tactacaata atacgatcta
      181 catatctggc aacatcttcc atgctgtgcg acaccagtat aactgtgatc ttacgttctt
      241 tctgaagcag tgctacctga tccaggatct catcgcgtcc tttcggatca aggcctgcgg
      301 tcggctcatc caggatcaac acctgcggat gcatcgcaag gatacctgca atcgccacac
      361 gccttttttg accgcctgaa agttcaaacg gtgatttctt ataatagctt tcatccaagc
      421 caacctgtgt cagtgctttt tttgcccgct cttccacttc ttctttggat aatcccagat
      481 ttttcggtcc aaagcagaca tcagaaaaaa catcgacttc aaaaagctgg tgctccggat
      541 actggaagac cagaccgacc ttacttcgca gggtacgcat gtcgtaacct tcctgataaa
      601 tattttctcc atcataataa atcgcaccgg aagttgcccg aagcagtccg tttaaatgct
      661 ggatcagcgt agatttcccg gagccggtat gaccgatgat cccgataaac tgtccgtccg
      721 gaatttccag actgatctca tcgagtgctt ttttctcaaa cgcatcgccc tcactgtaaa
      781 cgtagcttac tttttttaat ttcattgaca taatgctttt accaactcct ccgtacttag
      841 tacacattcc ggcagatcaa caccggcttt tctcagttcg tgtgccagaa gagtcacctg
      901 tggcacatcc agacggtatt ttttcagagt gtccacctga gcaaaaatct cgaacggtgt
      961 cccctgcata acgatttttc catcatccat cacgattact ttatctgcat agataacttc
     1021 ttccatataa tgcgtgatca aaatggcagt tactttttct acctgattta acgcacgcac
     1081 ggcacgaatc acttcttttc ggccgttcgg atccagcatt gcggtcggct catccagaac
     1141 gatgcatttt ggatgcattg caaccacgcc ggcgatcgcc acacgctgtt tttgtccgcc
     1201 ggaaagtttg tttggagaat ttttgcgata ttcccacatg ccgacacttt tgagactctc
     1261 ttcaactctc tcccaaatct cctcggttgg tattcccatg ttttccggtc cgaatccgac
     1321 atcttcttcg accaccgtac cgatgatctg gttgtccgga ttctgaaata ccatcccggc
     1381 cgttttacgt acctcccaga tattcttttc atcccgcaca tccataccgt ccacatacaa
     1441 ggtaccgtct gtcggtgtca aaatcgcatt gatctgcttg gcaagcgtgg actttccgga
     1501 accattatgc cccagaattg ccacaaaatc gccttttttc acatccagat tcacaccgtc
     1561 cacggcacgg atcgtctctt cgacttcccc attttcgtca tactttttat attcaaatgc
     1621 cagcttcgcc gcctcaatga ttcccatggt ccaccatact ttcctgtatt tttaagatac
     1681 ttttacgcaa gttttcggag tgcttttttg cactcgcatt ttattgtaaa ggatttagat
     1741 gttaattaca agtctcttcc agctgttttt ttaaatatct tccgactact gccgaaaaat
     1801 tatcaattct gcggatatag gcaaaatctt tgccgaaaat tctccgctcc gcctgaagat
     1861 ccccttcctc cccagtaaaa acaccgagta cggatacacc cgcatttcgc agatgccgta
     1921 cttccattgc cgtatctttt gtgccataat caccgaaata aggcctggga ttgcggctgt
     1981 tcgggcgatt gactacaata tcatttggtc ttccgtcact taacacgatc aggattttgt
     2041 tttcttcgtc tcttttcaga agccctgcgg ctgcagcacg cacagccagt ccgtcccggt
     2101 tgttggaaga gccgtaaaat tcaaaaatac gcaggtttgc ttctcttcca tcctcgtatt
     2161 cccgaaatct ccgcatgacc gtataatccc agaacgtgca gaaactcata acccggtgtg
     2221 ggattccaac attggtgagt gcctcactga tcataaaagc ctgcagtgcc acatggctct
     2281 gtctgcttct ctgggaaccg ctgccgtcga tcagcacttc aaccacaaaa tcggtattat
     2341 aacgcctgct ctcctgcaga aacaattttc tgttctctgt ccgtccgata ttccagaggc
     2401 gatttggcaa gattctacca gattctccca cataggtttc tttttcgttc ctggctacca
     2461 gtgcacgttt taaaacattg gtcagcatat caatattctg ttttgccatt ctgcggttct
     2521 gctcgtatac acgccagttg acttctttcg tccttctggc atactctgac tgggcgttga
     2581 ctttcaccat atttgcaagg atcccatctg taaaatataa agtacagtct gcatgtgccc
     2641 ctgtgcagat ctgatggttg atgcgttctg cctccagcgg tgtcaggtaa ctcctgccat
     2701 aattgagttc aatgtaactg ttcatttttg caattgcttc atcatccagc ccgatcacct
     2761 gcgtactgcc cttttgctcc tgttcctttt tttctttttc cttctcagaa aaagaagtca
     2821 tggcatctgc cagctgatgc tgcatcctcc gataagcatc ttccgccgct tcttcttcca
     2881 gaaaatcctg ccagtca
//
LOCUS       DAWDWH010000238         2869 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_12015_length_2869_cov_11.587420, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000238 DAWDWH010000000
VERSION     DAWDWH010000238.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12015_length_2869_cov_11.587420"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          739 a          727 c          592 g          811 t
ORIGIN      
        1 aaacacattt aaccatatta agcaatatat ttagttactt ataaattcct cctttccagc
       61 tatttagaaa attttctaat tactttatat atatcactct gttttcccgt tgtcaatcac
      121 tatttagaat tttttctaaa tagtttttta ggaacaagcc ataaaccatg ctgcacgttc
      181 acaggcgtaa ttttgcggct tgattttttc taattttcac ataccggaat cagaatttcg
      241 gtcacaaatt ttccggtgtc atttgtgatc ccatagtcga tgagggtgat ctctctggag
      301 aatcctgcga ttttgagctg atgttctttg atataggaga gaagtttccg ataatatttt
      361 tctgcctcct gatgtccgcc gcagaagcgg atcgatacgc tcagggtatc cggcaactgc
      421 tcgacttcac cctggtagtt ttcttcgtca tccagcacca gaaatacccg gtcataagca
      481 tccagttgac cgttttccag cttttcttcg gatatgccaa cacccacttt tccaaggaag
      541 gtcagagaat ccggctggtc tttttccaga cggcggatcg catattccag atccagatgc
      601 gaatggatct tcaggctgtc ccggatccag acgatccggc aggctttggt ctgctgcatg
      661 cggatctcat ccagcttcga tgaggccgca tcctggatct gatggattct gtggtcgatt
      721 ttccgttcta tgacctccag ctgacgcttc ttttcccgaa tcatctcttt ttgattttgc
      781 agtttttctt cgatcacctg aatgtcgcgg ttggcgagga aatctgcaat ctgctccagc
      841 ggcatatcga gcacccgcag atagcggatc gtattgagca cctcaaactg gcggatacca
      901 tagtaacggt aactgctttt ttcctcgata aactccggga cgataatccc aatccgctca
      961 taatgacgaa gcgtcccggt gctgatatgg aacatttttg ccacatctcc aatgcgaaat
     1021 aatttttctt cttccatgct tatcctgctt tctgccattc ataaacaacg tgttatctta
     1081 catctgatct atgctttttt gaaactctca ctgtgctatc tgctgtctgc acattctgca
     1141 atcttttatg ctgttttagc cagacgaatg ccgccacgca gatcagtgct gagagcaaag
     1201 aaccgcaggc agtcgcgatc cccatcggga aaagggtctg tgggaaccat tttgaggtca
     1261 ggtaagctcc cgggattcgc acgcacagga tcgcaacgat gttgtggaaa aaggaaatcc
     1321 cggactttcc gtaagcacag aagtaaccgc tgaagcagaa atggatcccg gcaaaaaagc
     1381 agtcaaaaat ataaccgcat atgtactggc ttccaaatgc gatcacagtc tgatcggatg
     1441 taaacactcc aacgaccggt gatgcgataa actggatcag gattgcgata aaaatgccga
     1501 atccgccggc gatcagcaga gcgtagtgaa gcgtctctct ggcacggtcg tgctttccgg
     1561 caccgatatt ctgtgccgcc agtgctgaca cggaagaaag catcgaggat ggcacaagaa
     1621 acagaaaact gatgatcttt tccacaatac cgacggctgc cgcctggttt aatcctcgac
     1681 ggttggcgat gatcgtaata acaagaaatg cgacctggat aaacccatcc tgaagtgcca
     1741 ccggcacgcc gatgttcaga atctgtccca tcactcgcct tttcggccgg agattctgct
     1801 ttttcagggc aatccccatc ttctttttcc gaatcataaa aagcgagatc acaacgctga
     1861 ccgcctgaga cagtgtcgtt ccaagtgccg ccccggcagc tcccatgtga aaagctccca
     1921 taaacagata gtcaagggca atgttaatca cgcaggcgat ggcgataaag tacatcggac
     1981 tttttgaatc accgagcccg cgaaaaatcg aactgatcac attgtaagcg atgataaacg
     2041 gaatgccgac aaaacagatc gtaaggtaga tcctagtacc tgccacggct tcctgtggcg
     2101 tggacataac ctgaaggatc ggattgacca gaagcagaag gatggcaaac atgcctgccg
     2161 ccaccacagc aaacagtgtg accgtatttc cgatggcatt tgccgccctc ctcttgtcgt
     2221 ttgctccgat cgcctgaccg atgctgacgg tcgttcccat cgcaagtccc acgatcataa
     2281 ccgtgagcat gtgcatgacc tggctgccga ttgagacagc ggtcgtatct gccacgcctt
     2341 caaactgtcc tatgataaaa agatccgcca ttccgtaaag cgtctgtaaa aaataagata
     2401 ataaataggg cagtgaaaag tacacgatgt tctttaagac actgcccgag gtaagatttt
     2461 tttccataag gctatctaaa gctcctttcc atgtccggca acgttttatt ctgccatttg
     2521 ttgctttatg ataaacctta caataattgc aaagtcaatg caagttttta aaataatttt
     2581 tgtactacaa agtctctttg attttatcca gctgctcttt cgaaatttcc gtcacctgca
     2641 aaatctcttc ttctgacata gttccaagtt tcaggagctt ttttgcaatt tcccggcttt
     2701 tttcttccat gccatatttt cttccccgct aaattccact ctggactcca cgtttcattc
     2761 cctgctgaat tccatgttct tctactaaat ccgataaatt acacatcaaa tgcatctcct
     2821 ttccgagctg atcgctcata tgtaactctt tctggctcaa tacacgctt
//
LOCUS       DAWDWH010000239         2854 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_12083_length_2854_cov_13.662022, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000239 DAWDWH010000000
VERSION     DAWDWH010000239.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12083_length_2854_cov_13.662022"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          886 a          520 c          784 g          664 t
ORIGIN      
        1 atttttatat cgacaggttc tgctaaatga taaaaagcct ggggaatgtg tcagactatg
       61 gagaagaaag gcaggagatg aaaatgtgtc agtatgacag agaatcaaaa aaagcggaag
      121 aatttatcaa tcacgaggag attcttgaga cgcttcgcta tgcggaagaa aataagcata
      181 atgaagctct gatcgctcag atccttgaga aagcaaaaaa gagaaaggga ctggaacaca
      241 gggaggcgat ggtgcttctg gactgtgata tcgaggagaa aaatcaggag atctacgctc
      301 ttgcagagca gatcaagaag gatttttacg gcaatcgaat tgttatgttc gcaccgctgt
      361 atttatcaaa ttattgtgtg aatggatgcg tatactgtcc atatcatgca aagaataagc
      421 atatcagccg caaaaagctg acacaggaag agatccgaaa agaagtgatc gcactccagg
      481 atatggggca taagcgtctg gcactggaga caggggagga tccggtcaac aatccgatcg
      541 aatatgtgct ggaaagtatc aagacgattt acagcatcaa acataaaaac ggagcgatcc
      601 gccgagtgaa tgtaaacatt gctgcgacaa cggtggaaaa ttaccggaaa ctgaaagagg
      661 caggaatcgg aacttatatt ctttttcagg agacctatca taaagaaagt tatgaaaaac
      721 tgcatccgac cggaccgaag cacaattacg cttaccatac tgaggcgatg gaccgtgcca
      781 tggagggggg gattgacgat gtaggcatcg gtgtactgtt tgggctggaa ttgtaccgtt
      841 atgaatttgc agggcttctg atgcacgcag aacaccttga ggcggtacat ggtgtgggac
      901 cgcatacgat cagcgtgccg cgtatccgca gagccgatga cattgatccg gaaacctttg
      961 ataatggcat tgacgatgat acgtttgcaa agattgtggc atgtatccgt atttctgttc
     1021 cgtacaccgg catgatcgtc tctacgagag aaagtcaggc ctgtcgtgaa cgggtgctgc
     1081 atcttggtgt atcacagatc agcggcggtt ccagaacgag cgttggcggt tatgtagaac
     1141 cggaagcgga agatgataat tctgcacagt ttgatgtcag tgataatcga tccctggatg
     1201 aggttgtaaa ctggctgatg caccttggtt atattccgag tttctgtacc gcatgttatc
     1261 gcgaaggaag aacgggagac cgttttatga gcctgtgcaa gagcggtcag atccagaact
     1321 gctgtcatcc gaatgcattg atgacattaa aagaatatct gatggattat gcatcagaag
     1381 ataccagaaa aatcggtctg aaactgatcg agaaagagat tccgaatgta ccaagagaaa
     1441 agacaagaga gatactggag taccacttaa aagagattga agacggtcag agagattttc
     1501 gtttttaatg caagagtgtc aggaggaaga agcctatgag tctgaatgca acgccggcgt
     1561 cagaacgtat ccatatcggg attttcggca aacgaaatgc cggaaaatca agcctgatca
     1621 atgcactgac cggacagaat ctggcaatcg tatccgatgt ttccgggaca acgacagatc
     1681 cggtttataa agcgatggag cttctgccgt taggtccggt ggtactgatc gacacgccgg
     1741 gaatcgatga tgaaggtgag cttggaaagc ttcgagtaaa gaaaagttat caggtgttaa
     1801 ataagacaga tattgtactc cttgtgtgca gtgcagttga ggggattaca gaagaagagc
     1861 agaaattact gaagcagatc gaaaaaaagc agataccctg tatccttgtc atgaataagg
     1921 cggatctgct ggaagagtgt caaaaagggc agcagttcaa agagagagca gaaaatgctg
     1981 catctgtgag aggacagaag attccggtat tgtgggtcag tgctgcaaaa aatcttcaga
     2041 tccgtgaact gaaagaaaag attgcttccg gaatagaaca gaaaaagcca gaatatcctt
     2101 tggtagaaga tctacttgag atgggagatt ttgcagtcct ggttgtgccg attgataagg
     2161 cggctccgaa aggcaggctg atcctgccgc aacagcagac aattcgcggc gtgctggaag
     2221 caggtgcaac agcggttgtg gtaagagacg atgagctttg ggatacgctt cagaatctcg
     2281 gaaaaaagcc aaagcttgta atcacggaca gtcaggtgtt tggcaaagtg gcaaaagaaa
     2341 taccgtcaga tgtaatgttg acttcatttt ccattctttt tgcacgctac aaaggcgagc
     2401 tggaagtaca ggtacgtgga gcaaaagctc tggatcatct gaaaaatggt gataaagtgc
     2461 tgatctgtga gggatgtacg catcatcgcc agtgtggtga tatcgggaca gagaagcttc
     2521 cgaactggat ttgcaaacac accgggcagg agatagagtt tcattttaca agcggcacgg
     2581 agtttccgat ggatctgtca gaatacagaa tgatcattca ctgcggcggc tgcatgctca
     2641 acgagcggga aatgaaatac cggatcgcct gtgcacagga tgctggtatt ccaatcacca
     2701 attatggcgt ggcgatcgct tatatgaatg ggattttgaa aagaagtgtg gaagtttttc
     2761 cggaaatatg gaaaacacta tagtaataga gataagtatt ttttcaagtc gaacgcacgt
     2821 gagacttggt gcgtgattct ggtcagcgaa agct
//
LOCUS       DAWDWH010000240         2839 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_12148_length_2839_cov_12.131825, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000240 DAWDWH010000000
VERSION     DAWDWH010000240.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12148_length_2839_cov_12.131825"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1391..1400
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          866 a          554 c          755 g          654 t
ORIGIN      
        1 aagacactgc agcttggcat cggtgatacc attcaggttt ataaggccaa tatgatcatt
       61 ccgcagatcg ccgaaaacct gaccaggagc ggaaacatag agattccgca gaactgtccg
      121 gtgtgcggcg gcgtaaccag gatacagagg acggcagatg tggagacctt atactgcacc
      181 aatccggagt gtcaggcaaa gaagatcaaa tcttttgcac tgtttgtcag cagagatgcg
      241 atgaatatgg aaggaatgtc ggaagcgact ctggaaaaat ttatcggaag cggatttatc
      301 catgaatttg cagatatttt ccggctttcc cgatataaag atgcgatcgt tgagatggaa
      361 ggatttggcg aaaaatccta cgaaaaactg atggcgggaa tcgggcaggc aagaaatacg
      421 acactgccgc ggctgatcta cagccttgga atcccgaaca tcggtgttgc aaatgcgaag
      481 atgatctgta aggcgtttga ttatgacctg gaaaagatcc gcactgcaga tgccgagacg
      541 atggcgggga tagatggcat tggagaggtg atcggagcag cggttgccga ttatttccgg
      601 gatgcgaaaa aggcagagca gctggatgat ctgcttgagg agatccatct ggaaaaatat
      661 gtgccaaatg aaacagaaca gatctttgca ggcaagaatt ttgtgatcac aggctcactg
      721 attcattttg caaaccgcaa tgagctgaaa gcaaaaattg aagaaaaagg cgggaaagtc
      781 acaggttctg tcacttcaaa aacggattat ctgatcaaca atgacacaca gtcatctact
      841 tcaaaaaaca agaaagcaaa agaattagag atacctgtca tttcagaaga agatttcctt
      901 ggtatgctgg aacagaggtg agaatagcat ggcagagaaa gaaaaaaagc agtcggtaca
      961 gacgattcag acaaagacga gggtagaaaa aggaaaaagc agtattgcca gtgtgatttt
     1021 cggcaggacc ggtattatca tactgctgat cctgttgcag tttggctttc tgatcgcggg
     1081 atacacgtat ctgcagggta agatgtattt catcgattct gtcgtgaagg cgggagcagt
     1141 tattcttgtt atttacttaa caaataatcg gagcaatccg gcttttaaga tggtatggat
     1201 gacgatcatg ctggtggcac cggtgtttgg tgtgctgctc tatatttttg tggagtttca
     1261 ggtcggagtg cggtggctca atgcaagact ggagcacatg cacgaaacga cgaagaaaca
     1321 ctggacacag gatgaagctg tgcaggagca gctcaaagaa gaagattctg aggtcgcatc
     1381 gctggcacat nnnnnnnnnn aatacggcgg atttccggca tatgaaaaca ccggcgtcaa
     1441 atacttcagc tgtggggaag ataagattga agaactgatc cgtcagctgg aaaaagcaga
     1501 aaaatttatt ttcatggagt attttattgt ggcgaaaggc tacgtgtggg atcgggtgct
     1561 ggaagtgctc aaacgaaagg cagccgaggg cgttgaagtg cgtttcatgt atgatggtac
     1621 ctgtacggta tcgcttttgc cgccgaatta cccgaaaaaa ctcgaacagg aagggattgc
     1681 ctgccgtgtg ttttcaccga tccggccggc tctgtccaca catcagaaca accgcgatca
     1741 ccgaaaaatc gtggtgatcg acggcaaagt ggcatttacc ggcggcatca acctggcgga
     1801 tgaatatgtg aaccgcaaga tccgttttgg gcactggaag gatacggcga tcatgctgga
     1861 aggagacgca gtgcgaagct ttacgctgat gttcctggag atgtggaacg tggccaaaag
     1921 cgaaaaagta gaagagtttg atccgtatct ggatgtcata tacgaaagag aaaaacattc
     1981 cggcgaaggt tttgtgattc cttacggcga cagtccgatt gacggagaac acgtaggaaa
     2041 aatggtgtat atggatattc tgaatacctc aaaacggtat gtacatatca tgacacctta
     2101 cctgatactg gataatgaga tgatgacagc tttgaaattt gcggcaaaac gaggggtgga
     2161 agtgatcatt attatgccgc acgtgccaga taaatggtat gctttcgtcc tggcgaaaac
     2221 gtactacaat gaactgctcg atgccggtgt gcagatttat gaatatacac cgggttttgt
     2281 gcatgcaaaa gtatttacaa gcgatgaccg caaggccgtg gtcggaacca tcaacatgga
     2341 ctaccgcagc ctgtatcttc attttgaatg tgcagctttt ttatatgaga acagtgaaat
     2401 cccggcggtt gagaacgatt tccaggaaac actgaaaaaa tgtcagaaga tcacacagga
     2461 agattacaaa aagcaaaaga gctttgacaa gatcgcagga agtatcctgc gtgtatttgc
     2521 accgcttatg tagaaatagg acgacggtgc cacagaaaac gaaacactga aggaggacaa
     2581 ttatcatgcc gatcaaggtt cagggcgatc tgcccgcaaa agaaatactg gaaagagaaa
     2641 atatatttgt gatggatgag aatcgggcga tgcatcagga tatccgtccg atccagatcc
     2701 tgatcgtgaa tctgatgccg ctcaaagaag agaccgagct gcagcttctg cgatcccttt
     2761 ccaatacgcc gctccagatc gatgtgacct ttttaacgat gaacagtcac gaatccaaga
     2821 atacctctac cagccatct
//
LOCUS       DAWDWH010000241         2813 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_12253_length_2813_cov_11.867658, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000241 DAWDWH010000000
VERSION     DAWDWH010000241.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12253_length_2813_cov_11.867658"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          867 a          551 c          707 g          688 t
ORIGIN      
        1 catgcctcac gctgatgctg ttccagaagt ttatccagtt ctttttgaaa atttatctta
       61 ttagccataa aagccatcct ttctgtaata cttataataa aaaataatat tgtcattaaa
      121 gaataccata cgaatggttc cgtacttcgt actcgtagtt gcagactttt gtccctgctg
      181 ttcttagttt atcacaatcc cggaaagcag acaattcata atcaaaactt ccccatgcat
      241 cataaaataa attagaaaaa aagagaagga gaccgtgatg tcagagcttt tggaagtgaa
      301 tcatgtcagc tatgcctatc acagcataga tggcgagacg gaaacattgg cggatatttc
      361 ttttcatata aaagaagggg aatttgcagc aattgtgggt ccgagcggct gtggaaaatc
      421 cacgctgctt tcactgatct gcggactttt aaagccggaa aaaggcgaga tcctgctcga
      481 tggcaaaagc atcgcaggcg aaaaagagcg gatcggctat atgctgcaga aagattatct
      541 gtttgagtgg aggagcattt acagcaatgt gatgctgggg ctggagatcc agaagaaaaa
      601 gagtgaaaaa tatacgaaac gcgtggatca gatgttaaaa gactatggat tgtgggactt
      661 tcggaatgta aggccgtcgc agctctcagg cgggatgcgg cagagggcgg cactgatccg
      721 cacactggca cttcagccgg aattgttgct tctggatgag ccattttcgg cacttgatta
      781 tcagactaga ctttcggtct gtgaagatat ttaccggatc atcaaaaaag aacagaagac
      841 ggcggtgctg gtgacgcatg atctctctga ggcgatcagc atggcagacc ggatttttgt
      901 gctgagcaaa cgcccggcag ggatcaaaaa agaaatcaaa cttgatttta atatgaaaga
      961 acgcacgccg atgcaggttc gaaatgcacc gcagtttcgg gattatttca atcttatctg
     1021 gaaggagctg aatgaacatg actaagcctt ccgaatcgca gaaaaactat atccggacgg
     1081 taagaaggag aagacgcacc gtgcagttca tgcgtctttt tcttttcctg ttttttgtga
     1141 ttttatggga agtggccgca gaattttcgt ggatcgacag ctttattttc agcagtccgc
     1201 tgcggatcgc aaaaaccgcc tggcagatgc ttcaggatgg aagcctgacc atgcacatcg
     1261 gtgtgacgct ctctgagaca ttgatcagct ttgccctggt ggttatctgc ggaatcctgc
     1321 ttgccattct gctgtggatg ctgccgatgg tatcggagat cctggagccg gcacttgtgg
     1381 tactgaacag cctgccgaaa tctgcactgg ctccgcttct gatcgtgtgg ctcggtgcca
     1441 acatcaaaac gatcattctg gcgggcatgt cggtggctgt tttcgggacg attttaaatc
     1501 tttatacctg cttttcgcag agttcaccgg agaaagagaa actaatctat acgcttggag
     1561 gaacaaaaaa agatacgctt ctgaaagtcg tgctgcctgg aacggtgccg aacatcttaa
     1621 gcattatgaa agtcaacatc ggactgtgtc tggtcggtgt cgtgatcggg gaatttatcg
     1681 gggcgagaag aggacttggc tatttaatca tctacggctc ccaggtgttt aaactggact
     1741 gggtgctgat gtcgatcgtg atcctctgcg tgatcgccat gcttttatat gcactgcttg
     1801 gcaggctgga agcagcctgc ctgaaaagat tataaaaaat acgatataca gaaaaatcag
     1861 agtgctttgc ggtgtttttt gcggtactcc gccggagaaa gcccggtttc acttttgaac
     1921 atccgggaaa atccggaaaa attggagtac gaaagcgagg aggcaatgtc gctgatgctt
     1981 cttgtagtgt cggtcagaag acattaaacg gaacttatgc atgcgaaaac tttgatctgc
     2041 tgacatctgt cccacgcggt gaatggggat ttgacggtat ggtcatgaca gactggggtg
     2101 caggaaacag agccggtgtt gacaccatga tgcatgcagg aaatgacctg gtaatgccgg
     2161 gaagaacaca ggatcgtatg attgcagcac tgcagggaaa tccagttgga acaactgctg
     2221 atccgaatct tgataaaaca ctggttcgcg gtgatatcca gaaatgtgta tccagggttc
     2281 tgactatgat catgagatct tctcagttcg gtaaaatgaa ttctaaagta gatgtaaaag
     2341 cacacacaga gacttatgat aacctggtaa cttactcttc cgttaagaaa gaagaagtaa
     2401 agacaaccgc agtgaagaaa ctggaagacg agctgaagaa agctcaggaa aaagcagaca
     2461 aactggaaaa agaactgggt gaagcaaacc agaaaacaca ggctgcaaat aaagaagtag
     2521 aaaaactgca ggcaaaactg gatgtacgca caaagaatat caggcttaag agtgcaaaat
     2581 ctaaaaaagc aaaacaggca acgattagat gggcaaaagt aaaaaatgta gatggatatg
     2641 tagtagaata ttctacaaaa gcaaacatga aatctgcaaa gaagaaaaat gtaaaggctt
     2701 ccacaatcag ccttactgtt aaaaacctga aaaaaggcaa aaaatactat gtacgtgtaa
     2761 gaggctacaa agtcatcgac ggacagaaag tttacacaca gtacagtgca aag
//
LOCUS       DAWDWH010000242         2738 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_12609_length_2738_cov_10.336564, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000242 DAWDWH010000000
VERSION     DAWDWH010000242.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12609_length_2738_cov_10.336564"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2194..2203
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          558 a          762 c          556 g          852 t
ORIGIN      
        1 cagtcataca tctgcttatc gtttgcggat ggatcatggc ggatatcgat cagcagaaag
       61 actgctttta actgtgagga tttatgcaga taatcttcga tcatcttgcc ccatttctgc
      121 ttttcgttct gcgaaacttt ggcataacca tagcccggaa ggtccacgag ataaaccttc
      181 tcatccgcag acaccatgcc ctgtgcttct ttttttgtct ctctgcgtcc ggtcacggca
      241 tagaaattga tggtctgcgt ctttcccggt gccgagctgg tacgtgcaag agactttcgg
      301 ttcatcagcc cgttgatcag agaagatttc cctacatttg actttcctgc aaatgccact
      361 tctatttgcc cgttttccgg caatttgctg gtaatgccac agacagtttc cagttctgca
      421 ttctttacga tcatctcgtt tcctcctgtt ttccttttgt tctatttctg actgcaaagt
      481 gccagttcca gcacttcatc catattttcc ataaaacgga tatccagtcc ttttttgatt
      541 tcgccggata tctcctgcac atccacacgg tttttctccg gcacaagcac cctgtgcata
      601 cctgcatttt tcgctgccag cagcttttct ttgagtccgc cgattggcag cactctgccc
      661 cgcaatgtga tctcacctgt catggcaaca tctgcacgaa ccgggatctc cgtgatcgcc
      721 gacaggatcg ctgttgccat cgtaatacct gcggaaggtc cgtcttttgg aaccgccccc
      781 tccggaatgt ggatatgtat atcgtgttcc cggaagaatc cccggtcgat gttccactta
      841 tctccgacag atcggatgta gctgattgct gtctgtgcgg actctttcat cacatccccg
      901 atctgaccag ttagcttgaa atcgcctttg cccggcatgc agtttacttc cacctgcaag
      961 gttacaccgc cgacactggt ccatgccaga ccgcgtacga tgccaacctc atctttttca
     1021 ttggcttttt ctacgtggta acgttctttt ccgagataac ggctcaggtt gctctctgtg
     1081 atgcggactg ccgttttttt ctcttcgagg atctcttttg ccgttttcct gcagatctcg
     1141 ccgatgcgac gctccagtcc ccgcactccg gcctctcttg tataagatgt aatcatcttt
     1201 ttcagagcac cgtccgaaac ggtaaaggta taattttcca atccatgttt tcggatctgc
     1261 tttttcagaa gatgttcctt tgcgatatgc agtttctcat tctccgtata gctggagatc
     1321 tcaatcagtt ccatacggtc aagaagcgga cgcgggatcc cctgcacatc gtttgccgtt
     1381 gcgataaaca aaacttctga aagatccacc gggatctcaa tataatgatc gcggaatctg
     1441 acattctgct ccggatcaag gacttccaga agagcagatg aagtatcgcc tttgtaatcg
     1501 ctgctcacct tgtctacttc atccagcagc atcagcggat ttttgacgcc tgccattttc
     1561 agtccgttcg caatacgccc gggcatcgct ccgacgtatg ttcttctgtg gccgcggatc
     1621 tcagcttcat cccgcacacc gccaagacag atgcggacat attgtttgtt cagtgcttcc
     1681 gcaatggatt ttgcaatgga agtcttaccg gttcccggcg ggccaaccag acagaggatc
     1741 ggactgtccc ctttcctggt cagcgtccgg actgccagaa attctaatac acgctctttt
     1801 actttctcaa gtccgtagtg atcctgatcc agctgctttc tcgctgcgga aatatcttcg
     1861 ttatcctcag acattttatc ccacggcatt tccagcagtg tctcgatata gccacggatc
     1921 actcctgctt cagaagtatt gcctgccgaa ttagaaaagc gtttgatctc ttttcgtatc
     1981 ttctctttga cttcttcgcc tgccgtcagc ttttccgttt cttccagaaa atgctgtgca
     2041 tcatccagcg ttgtgtcatc gcccagttct tctcttatta atttcagctg ttctcttaaa
     2101 atatattctt tctgattttt gtcgatacgt gcctttacct tttcctgaag ctgcactttg
     2161 atccgcatga tattctcttc gtttgcaaga atcnnnnnnn nnngcggtca tacagaaaaa
     2221 cagcttccag aatcctctgt ttttcttcat agtacagtgg aatatggatc gcgatctgat
     2281 ctgtcagatg ctccagatct tcgatctcca tgatctgacg caccagctcc tggctgatct
     2341 tgccgtttgc ctgtgcgtac ttctgaaaca gttctttcag attgcggatc atcgcctgct
     2401 gtccgacttc atcgcctttt cccggataat cctgatcttt gacatccggg aacgttgcaa
     2461 ctttggcgtc cagatatccg gcagatgctt ccatttccag caattccgca cgctccaggc
     2521 cttctaccag tacgcgtata atatttttcg gtaatttcac aagctgtttg atctccacaa
     2581 cggtaccaat gtgatacaga tcatcgatcc ccggatctga agcttccgga tctctctgtg
     2641 ttaccacaaa cagtttctgg tccgctgtca tcgcctgttc gatcgccttt atcgatttat
     2701 cccggcttac atcaaaatgc acgatcatcg atggcagg
//
LOCUS       DAWDWH010000243         2712 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_12723_length_2712_cov_9.984945, whole genome shotgun sequence.
ACCESSION   DAWDWH010000243 DAWDWH010000000
VERSION     DAWDWH010000243.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12723_length_2712_cov_9.984945"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          578 a          699 c          493 g          942 t
ORIGIN      
        1 gatcatagag agcactctat tctgactcaa tcttttcaat ctccttcttc aggctgtcat
       61 atatctccgg caagatgccg gttttcagtg aacgctcgat cccgtgattt ctgatcgctt
      121 tttcaaggca ttccccggat ctgcaaatat atgcacctct gccgtttttc tttcctgtcg
      181 catccaggat gatcccttcg ctttctgttt tcaggatgcg gatcatctcc cgtttgcttt
      241 tcatttcctg acatcccacg catttccgca tggggatttt tttaacctga ctcatgtttt
      301 actcctgatc gccctcttca ttcagcatat cttccgttta tttgtcagaa gtttcttcgt
      361 atgcttcttc ataatcaaaa gtatcctcat ctgctgcttc tgtatcagcg tcttcttcgt
      421 aatcgccctg catcagatat tcatccagct cgccggattc tcttacctgt gtctcactct
      481 tgatatcgat cttgaatccg gtcagttttg ctgccagtct tgcattctgt ccttcttttc
      541 cgattgccag agacagctga taatccggaa cgatcacttt tgcggtcttc tcatcgtcat
      601 ctgcgataac agagattact tttgccggac ttaatgcatt ctcgatcagg attgccgggt
      661 tttcatccca gttgatgata tcgatctttt ctcctcgcag ctcttctacg atcgtattga
      721 cacgcacacc gttcatgccg acacatgctc cgaccggatc tacatccgga ttgttggacc
      781 agactgccat ctttgttctg cttccggctt ctcttgcaat actcttgatc tctacagtac
      841 catcctttac ttcggttact tcggactcaa acagacgttt taccagctcc ggatgggtac
      901 gtgatacaac aactttcggt cccttcggtg tgtcttttac ttccagaacg taaagcttga
      961 tacgctcggt cgggcggaac acttcacctt ttaccatttc attttccatc aggacggtat
     1021 ctactttacc aaggtttacg ctgacatttc tgccgacata acgctgaaca ataccggtaa
     1081 tgatatcctt ttctttctga taatattcct caaaaagtac ttttctttcc tcttcacgga
     1141 ttttctgaag gatcacgttc ttggcattca tggtagcgat acgtccgaat tctttggatt
     1201 tgatctctac gtttacgata tcaccaagct catatttgga gttcagctgt tttgcgtttt
     1261 ccaggctgat ctccatgatc ggatcttcta ctttctctac aaccgtcttt tctgcgtgca
     1321 cgttgaactc acaggttacc ggatcaataa atacctttac attgtctgct tttccaaagt
     1381 ggtttttaca tgcctgaagc agggactgtt cgattgcttc cagaagggta tccttgctga
     1441 tgtttttttc tttctccagt acatttaatg cttccaacaa ctcgctattc attttctttt
     1501 atcctcctat tatgtttaaa aatcaaacgc cagtcttacc agtgctgtct cggaacgctg
     1561 gaacgtcatt tcgttgtctt ctatcactat ggtgaagctg tctttgttga attcttttaa
     1621 gatacctgtg aattctttct gtttgtcgat ggcacggaaa gtgcggactt cgatctcttt
     1681 tccgatgctt ctttggaaat ctttgtcctt tttcagcgga cgtccaagtc ccggggaact
     1741 cacttccaga atgtaggaat cttcgatgta gtcttcttca tccagtttgt cactcaacgc
     1801 tctgctgatt acttcgcagt catctactgc aatcccgccc ggtttgtcaa tataggcacg
     1861 cagataccag ttaccggcct cttttacgta ttctacatcg acaagctcga acccgtgtgc
     1921 atccacaagc ggctgcagca gttcttctgt ctttgcttca taagtctctc ttcttgtcat
     1981 tcgcttcacc ctttcttata caacaagaat gagtgggcga actgcccact cacactctgg
     2041 taatcttcta tcatcactcc aattataaca ctactgctgt taaaatgcaa gactttgttg
     2101 aaaatttctt attatttaat ttttgcactt tttgcctgtt tttttatcag gaaaccctcg
     2161 ttttgactgc ttttgcatct cgtttgctga tccgcaggcg atttaaatgt acttctttgt
     2221 ctttgtatat agcgatgtca tctccgttcg gtgtgagcag atgtacctgc aaaagctgat
     2281 ctttcgcatt catcttcata ccgcggacac cgattgccgc cttctttttg accggaactt
     2341 cttccagcat aaaacgcagg aaatatccat ctatgctttg caggacaatc tgttcttcct
     2401 caccgatcag taaaactgca atcagtttat cgccatccgt aagttttgtg gatgcgatcg
     2461 tccgtttgga gacatcaaat tccgtcccct ctaccttttt gatcatgccc tgctgcgttg
     2521 caaacaacag atttgtgctc ttgaccgcat tcaggccttc gatgcgaaga atttcttctt
     2581 ccgtgctgga ataattgcag agattgtcaa gcggtgtacc tttgtcacga aaacgtccat
     2641 aaggaagatc tgccactttc agaagatgca gacgtccggt atccgtaaaa atacagagct
     2701 tgtcggtatt ca
//
LOCUS       DAWDWH010000244         2699 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_12772_length_2699_cov_24.855522, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000244 DAWDWH010000000
VERSION     DAWDWH010000244.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12772_length_2699_cov_24.855522"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          558 a          745 c          608 g          788 t
ORIGIN      
        1 aaatattctg ccatcgtaag tccggtaaga ccgacacgca tacgtgctcc aggcggctcg
       61 ttcatctgac cgaacaccat ggtggtctta ttgataactc cggattcctc catctcgtga
      121 tagaggtcat ttccttctcg tgtacgctca ccaactccgg taaatacgga atatccaccg
      181 tgctcggttg cgatattgtg gatcagctcc tgaatcagaa cggtctttcc tacacctgct
      241 cctccgaaca gaccggtctt accgcctttc tgatacgggc aaagcagatc gactaccttg
      301 ataccggtct ccaggatctc ggtagatgtg gactgttctt caaaagaagg tgcttttctg
      361 tggatcggta aatattcgac gccttccggt gccggctttt tatcaatcgc atctccggtt
      421 acattgaaga tacgtccaag tgtcttttct ccgaccggaa cggtgatcgg tgcaccggtt
      481 gcgatcgctt cggcaccacg aaccagccca tcggtcggtc ccatcgcaat gcatcgcacc
      541 gtatcgtcac ctaagtgctg tgcaacttct gccgtcagca caccgccgtc cggagctgcg
      601 atatggatcg cgtcgtagat ctttggcagc ttgccttccg gaaatttaat atccaggacc
      661 gcaccaatga tctgcgtgat ctttccttga atttgttctg ccatgtttcc tcctagtaat
      721 gccgcatatg ctgcggcaga cgtttctatt caacagcctc ggctcctgca atgatctcgg
      781 tcagctcctg cgtgatcgag ccctgtcttg cacggttgta ctgcagttcc aggctttcca
      841 tcatgtcctc tgcattgctc gtagctgcat ccattgcctg catacgtgca ccgttttcgc
      901 tggctgccgc ctcgtttaac gctccgtaca gaagacttgc gatatatttt ggtataatca
      961 tatccagggc ttcttcctcg cccggcttgt agttcatcgg cgtattccgc tctgcatcgg
     1021 acagctcttt gtcactgaat tccaccggaa gcagctttaa aagtttcggc tcatgtacca
     1081 ccgtgttttt gaagctggta tagacaagat aaatctctcc gatcttctct tcctcgaaat
     1141 ctttcaggac tctctggctg atctccaccg catccgaata cagaggggaa ttgatcacct
     1201 cgctgtaatc ttctgcgatc tcatagcctt ttcgttccag tgcttcatgc ccttttcggc
     1261 cgatgttata gatcagaagc tcttttcctt ccagttcgcc ctgcgtgagc attttcacga
     1321 tgttggagtt gtaaccgcct gccagtcctc tgttggatga gatgacgatc acaccctttt
     1381 tccccgattt tccgcttttc agatacgggt gttccacaat gccggtacgt gcaagcatac
     1441 tggtcaccgt ggcatacata tggttaaaat acggtttgca ttcttccgct ttttccctcg
     1501 tcctctgaag ctttacggtg gaaaccagct tcatggcttt tgtgatctgc tgtgtgctgg
     1561 cgatactgtt tctgcgcctc tgaatatctc tcattgaagc catggtatca tcctcctgct
     1621 ttcttttccg tcatcatgct gctttattca gctgcagaaa attttgcttt gcactcaccg
     1681 atcgcctgga tcagtttctg ctctgcttcg tcggaaagta ccctggtctc tttgatgctc
     1741 tccggaatct ccggatattt ggtcttgata tattcaaaca gtgcttcttc aaactccaga
     1801 actttttctg tcggaatatc cagcagatgt tttctggtag ccgcatacag gatgataacc
     1861 tgatattcta ccggaaccgg tgcatactgc ggctgtttga ggacttcacg gatacgctcg
     1921 ccctgtgcca acttttcttt ggtatcggca tccagttcgg aaccaaactg tgcaaaagat
     1981 gccagctcac ggaactgtgc cagctctact cggatcgggg cagcaatctt tttcattgcc
     2041 ttgatctgag cggaacctcc aactcgtgat acagaaagtc cggcatttac cgccgggcgg
     2101 aatccggcat tgaacatctc tgtctccaga tagatctgac cgtctgtgat ggaaataacg
     2161 ttcgtcggaa tataagcgga aacgtcacct gcctgtgtct cgatgatcgg cagtgccgtc
     2221 agagatcctc cgccaagttc atcggaaagt ctcgccgcac gctccagaag tctggaatgc
     2281 agatagaaaa cgtctcccgg ataagcttca cggcctggtg gacgacgcag aagtaaggaa
     2341 agtgtacggt aagctgtcgc atgtttggac agatcatcgt aaatgatcag tacgtcctct
     2401 ccattctcca tccattcttc tccgatcgca catccggaat acggtgcgat atactgcagc
     2461 ggagcaagtt cactggaagt ggatgctaca acggttgtat aatccatggc tccgttttct
     2521 tccagtgtct ttacgatatt ggcaacggtc gatgctttct gaccgatcgc tacataaata
     2581 cattttacat tctgaccctt ctggttgatg atcgtgtcaa tcgcgatcgc tgtcttaccg
     2641 gtctgacggt ctccgatgat cagctcacgc tgtcctcttc cgatcggcac catggagtc
//
LOCUS       DAWDWH010000245         2696 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_12789_length_2696_cov_8.604317, whole genome shotgun sequence.
ACCESSION   DAWDWH010000245 DAWDWH010000000
VERSION     DAWDWH010000245.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12789_length_2696_cov_8.604317"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          984 a          413 c          630 g          669 t
ORIGIN      
        1 ttacgaggtc tgcgaagcag gaagcccatt ggctttagac aatgggtagt tcacctgtag
       61 aataaaggag gtatctatga agaacataaa ctgggctgta cttggtacag gtgttattgc
      121 aaatgaaatg gcagttgctc tgaaaaagaa tggaaagaat atttatgcag tcggcaatcg
      181 aacccatgaa aaagcagtta tttttgctga aaaatatggc atcggcaaag tctatgcgga
      241 ttacaatgaa atgttcaccg acccggatgt tgatgtgatt tatattacaa caccgcacaa
      301 tacgcatctt aattttatga aaaaggccat tgaaaacggc aaacatattc tggtggaaaa
      361 atcaatcacg ctcaacagcg acgaactgaa tgaagcattg acacttgcag aagaaaaagg
      421 tgttatcgtc ggggaggcaa tgacgatttt ccatatgccg atttataaaa agctcagaaa
      481 aattctggag agtggggcac ttggaaaagt gaatctgatc acgatgaatt tcggcagttt
      541 taaagagtac aatatgggca accgcttttt taaccgcaat cttgccggcg gtgcaatgct
      601 tgatattggt gtttatgcac tttcttttat ccgctggttt atggattcga aaccggatca
      661 gttattgtcg caggtgaaac aggcaccgac caaagtagat gagcaggcgg gattattatt
      721 gacgaatgcg gaaggtcaga tggcagccgt tatgctttct cttcattcga aacagccgaa
      781 acgtggcatg atttcctgcg agaaaggcta catagaggtg atggaatatc caagagcctg
      841 ggaggcaaaa atcacttata ctgacagcga aaaaacagag ctgatcaaag ccggtgaaaa
      901 tgcagacgca cttgcatacg agctggcaga tatggaaaaa gcaataaacg gtgatccatc
      961 ctgtatgcat ctggattata caaaagatgt tatggatatg atgacagagt ttcgaaaatc
     1021 ctggggattt acctatccgg aggaagaaaa ataattttcg actgtgataa cagaaaaaca
     1081 gaaattccca aaagactgta ccgttgtatg aaacagagag tgaatatttc agaaggaaag
     1141 aatccaggaa cgacagttaa gaaaagtgga ttctgcttag aatttttgct gcatgggaat
     1201 atagcaacga ttacaggata aatttgcgaa aggatgtaca actatgaatt atacagaaag
     1261 agcaaaacag ctcggttttt cagctgcagc tgttatggac acgaaaaatc tggttttcaa
     1321 accggagtac cgcgttttct gcgaggagaa tcgatgcgga aattatgatc ggaatcctgc
     1381 ttgtccaccg gaaagcggaa gcgtggaaga aatgaaagaa aaagcattat cctacaacaa
     1441 aacgcttgtc ttacagacaa cgcaaagcaa tgatatggat tataaaaaag caaaacttgt
     1501 ccacaacagg ctgacagaat ctctggcaga agagatgaaa aaagacggaa aaagtgatct
     1561 tttgatcatg actgccggtc cgtacaggca tcattcctgt atgtcagcat attgtgtgga
     1621 tgctcaggaa atggcagatg cagtagggat gacctgctgg ggaaaagatg gaaaagttcg
     1681 ctatttttcg caggttcttt ttcatgatac cggaaagtaa tatcgggcag aaggaaaaga
     1741 cataaaagac ggttacagta tatgtttcat tcggttgatt ggatacggaa aaagaagaaa
     1801 caaaaagatt gacatgcatc acataaacta ttataataat cacaaaacag aaacaaaatt
     1861 tgagataaac gaagattcga tattattctt tattcacatc caatgccatt cggcaggaaa
     1921 atccaacaaa atagaagacc tgcacgaagc ggtatgataa atgatatgac ttgtatttta
     1981 cagatatcag ttttacatca aaatttccat gctgcttcta atgtgcagaa aaacaaaaag
     2041 gagaagctta tgggagaaaa ggacattaca gagaaaatac tggaggatca taatgatgtg
     2101 tttgctgata ttataaatgg tcttgttttt gcaggagaac agaaaatcac tggtatgcaa
     2161 aaaagaatat taaagcattg atgaagattc cagatggaat ggaaaagtat attaatgatt
     2221 ataaaattca gattttcgag gttgcatggc tgacagaaga agaaatcgaa aggtttcaaa
     2281 gcgattttcg tattgtggca aatttttttg cccagaaaag aaaaaataaa gattatatac
     2341 cggatgatcc aacagaaatc aaatatgtag atgaagtctt aaaacttctc aatgtgatga
     2401 ccggagataa acgctatgaa aagatatttc agaaaaagaa aggagtgcga agtatgtgtg
     2461 atgtagcaga aagattggaa aaaatgggca ttgtaaaagg aatcgagatc ggaagactgg
     2521 aaggaaaaga agagggaaaa gtagaaggaa aagcagaagg aattctggaa ggcaaaatgc
     2581 aggtatatag aaatcttctc aaaaagggat ttacggaaaa agaagcaagg gaaattacag
     2641 aaatttcgtg atatgtcggt tgttcttttc ctttacatcg ctgcaaaaag ggtata
//
LOCUS       DAWDWH010000246         2689 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_12812_length_2689_cov_11.497342, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000246 DAWDWH010000000
VERSION     DAWDWH010000246.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12812_length_2689_cov_11.497342"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1546..1555
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          651 a          679 c          536 g          813 t
ORIGIN      
        1 aaaaatcacg aatgtaggtg gttttaccgc tacctgtgta atgtaataca gacgcagacg
       61 tttgccttta tctgaaggcg gctgctgcat ggccactgcc tctgtcatga tctcgttgag
      121 cacaccggtc tgtacacgca tggtctgatt ttcaatgatc gtatcgacca tgtcaaacag
      181 ttttaccagt ctctgtccgg tcagtgcgga gacaaacata atttccgcat atggcatata
      241 agaaagtgta tttcttactt cgttcgtata tttataaatt gttttatcat ttttctcgat
      301 tgcatcccat ttattgacta cgatcacaat gcccttgcct ctgtcatggg caataccggc
      361 aatcttggca tcctgttctg tgatcccctc tacggcatcg atcaccatga caacaacatc
      421 ggcacgttcc acagctgtga ccgtgcggat gatgctgtag cgttccagtt cttctttgat
      481 cttgcttttt cttcgcagtc ctgcagtgtc gataaagaca tattctttgc cgttccaggt
      541 aacagccgta tcaatcgcat cacgtgtcgt tccggcaata tcggatacga tacagcgatt
      601 ctcccccagc agtctgttta tgattgaaga cttgccgaca tttggcttgc cgacaacggc
      661 gatacgcggt ctgtcatctt cttcctcctc ggtatcatgt tccgggaaat ggcggatcac
      721 ttcatcaagc atatcgccga tccccagttt ggaagatgcc gagatcggga tcggatcgcc
      781 gataccgaga ttgtaaaact cataagtatc tgccataaat ttctggaaac tgtcaacttt
      841 gttcacgacg aggatcaccg gctttttcgc acggcggagc atatctgcga cacgtgcatc
      901 tgcatctacg agtccctgcc ttacatccgt cataaaaatg atcacatctg cggtatcgat
      961 cgcgatctgt gcctgttcac gcatctgtga cagaatgata tcgctgctgt ccggctcgat
     1021 accgccggta tcgatcatgg taaaattttt gccaagccac tctacgtccg cataaatacg
     1081 gtcacgtgtc acgcccggtg tatccttgac gatagaaata ttttctccgg caagaatatt
     1141 aaagagtgtg gattttccta cgtttggccg ccccacaata gctacgattg gtttactcat
     1201 atttattcac ctcatctgta taggaatgtt cgattgtctc tgtttccaat aaaagggcct
     1261 cgatcagtgc ccgaccgctt gattttacaa tattaatgtt cacttgtaaa gatttttcca
     1321 gttcccccac cgtcacatca tccagaaaaa catcgtatcc ggtacggaga atattgcacg
     1381 gaagcagcag ctgctttcca agttctttgc cggtaagctg ttttttcaga tcctgtccgg
     1441 tcagaagtcc ggagaccgtg atcttttcac cgaaaaactc gttgacgatc ggatagatgt
     1501 ggatggttac gttcggatat ttttcgcaga gctggtctgc gatctnnnnn nnnnncgatc
     1561 tgctgtatga caggtgccgc cagcagtccg gttgcaaggg aaacggtatg tttccggtca
     1621 tcgcctgtcc ggttttccat ctcttctttt acttcatcaa ggagcagccg catcattccg
     1681 acgccattct ccagctgaag atagccatca tactgttcgg ccggtggaaa cggctgttcg
     1741 gccaaaatat accattcatc cgatgcatgg ataaaatgga tgccgtattc ctgacagacc
     1801 ttttcctgcc atccatggat cagatccagc actttctttg catcttcttt atcaaaaggt
     1861 tccagcggaa aaagcccgtc ccgatacttt gacagcccaa ccggaacgac agaaacactt
     1921 tgcaggtacg gaaggtattt tgtcagctca cgtatgctga aatccagctc tgctccatca
     1981 ttgacgcctt tgcacagaac gatctggccg ttcatatgaa tccctgcctc atagaaacgg
     2041 tccacttttt tcagagcctc cccggcaaat cgattgtgca gcattttgca tcgaagatcc
     2101 gggttcatcg tctgaaacga aatattcatt ggttccatat gataatatac aatgcgatca
     2161 atgtcatgat ccgacatgtt tgtcagtgtg acataatttc cctgcaaaaa agaaagcctg
     2221 gaatcatcat ctttaaaata aagtgtatca cgcatacctt ttggcatctg atcaataaag
     2281 cagaaaatac atttgttatg gcaagaacga tagttatcca tcagaccatt ttcaaactca
     2341 atcccgatat catcttcata ttctttttct atctcaagct cccattcttc cccgttcgat
     2401 ttgcgtatca gaagctctac atattcatca ttcatcagat aatgataatc cagaatatct
     2461 tcgatctctt tcccgcttac agaaaccagt tcgtctcccg gttcaatttc cagttcttct
     2521 gcaatgcttc ccggcagtac ttttgatacg atatgtttgc ctttttttct catacttttt
     2581 tccttttctc tctctgtcag aaatatcttt tctgcacttt gtgaactact cggcaactga
     2641 gttaccagag catctgaaat ctggcgggat accgtctttt ttcagggtg
//
LOCUS       DAWDWH010000247         2687 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_12821_length_2687_cov_7.204027, whole genome shotgun sequence.
ACCESSION   DAWDWH010000247 DAWDWH010000000
VERSION     DAWDWH010000247.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12821_length_2687_cov_7.204027"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          722 a          696 c          535 g          734 t
ORIGIN      
        1 tcgtccagtc tttttttaac aaaccggatt cttttgtctt tttctgtccg gaaatgcctc
       61 taagatttgg cttcttcgct tcgccaggat cctttcaggc cttttctgcg gacgctgaaa
      121 tgacagtaca tggctgtaac agcactgacg atcagcagaa aatagaaatt cagacctctt
      181 gtcaggcaca tggacgccgg aagcagtgcc cctttaaaga cctgtccaaa ggatgctttg
      241 tacatgatct ctgtgattcc ctgtgctccc ggaagcggca gcatatcaac tgcaatataa
      301 acagatgcct gaaggatcat gatactgagc acagattctc cggtaagtcc catgcctttg
      361 tagatcagcc aggtcataaa aaatacactg catctctgga taaacgtcag gatcagcaca
      421 aatgcgatct tgtttttatg cccgatcaga aatacaactg cttcatggta ctgatctgcc
      481 atctcgatca gtttttcttt tcgttttcct gattttttga gaatgtgcag ttttttgagc
      541 actgcttcgc cgccaagtac cagactctta aagcagcgcg ggctgatcat gacaaacagc
      601 aggatcacca ccagtgccgt attcagaaac agtccaagat aataaagata aatatagttt
      661 ccaagatatc ccatcagctg cgtgcggtaa aaaagcaaaa tggcaacgcc cataagagcc
      721 agtaccagtt tgtataccac cgccaccgtc ataagcacca cgctgctgtc cgatacttta
      781 tgcccttctt tctgcatgta ataaagctgc atcggctgtc cgcctgttgc agacggtgtg
      841 ataccggaat aaaaaaatcc cacaaacgaa tatttgatgc aggaaaaaag aggggttttc
      901 ccctccagac tgccaagaag ataccagatc ataatgcctt ccgcggatgt aaagaacaaa
      961 gccgtaagag ctgcagccac cagataaacc gggcttaaag tctgcatggc agcaactacg
     1021 gtattcatat catttccctg aaaaataacc acaatggtag cggtcaatag taccaggaaa
     1081 aacgccgcac tgatcaaatt tttacgattc ataagctgct cctatcatta aataaacctt
     1141 tctgttaaaa tcatgcagag atccttcgaa aatggtttct gtctgttcca gttcgtaaaa
     1201 atcccttggt gtcacaagcg agaaatgctt ctggaaaagc gaaatgccat tctgctccag
     1261 gatattcgtc acaaaagaag aacaggtata atgattcttc tgataaaacg gtatctgaaa
     1321 caggatgaaa ggcagtccga taatacagta gtgatatcgc tttctttgtt tatagcactg
     1381 caaaagctgt cttgcaatac tttttttctg ttctgccgta cactgcatac ggactacttt
     1441 atattttgca tgcggaaata ctgcctcgat ctccagcgta ttttcttttt caaagccggc
     1501 gatcagcgga acggccggat tccttctccc cacactgtag gcctctctta gatcggcatc
     1561 catcgaaagg accacatgca cataagggat cccgatcacc ctacggatga tggacgcaaa
     1621 gaatcccggt gtatccacaa aagccagata aatctgttcc atagcttctc tccttccgat
     1681 cagttaaaat gatagcactg ttttgccagg cgataacctg ccagtgtaag tgccgtccct
     1741 gcttttccag gcagataagt aaaaccgctc acggtcccga agcgaagctt ccgataaaga
     1801 ccgtttcctc ttcttctcat atactcccac agttcgtttc ttttttccat tgcctcttcc
     1861 gttccgatca gcaaaagatg aatggaagaa atcgacagca tgattgacag attccgtacc
     1921 agataatccg ccagttttgg atgttccttc tgtacttgct tcaaatccgc acacccggaa
     1981 accatttttg tgaccagaat ctgttggtcg atccttttca tcagcacctg ctcattgacc
     2041 gactgatcct ctcttccaag gaaataatgg tagagatcca gatccaggta caggatcgtc
     2101 attacatacg gaagcggctg gttggcaaac agattgtcca cataaaacgt atgccgcggc
     2161 aggcgtacgc ctgaccttcg aagtacttct gtccggcaga tctgagcgtg catcacaagg
     2221 tactgcgatg gatgaaattt cccgatggta tcccaagtac agacttttcc attgcgaaat
     2281 acattctcat agcccatccg tttctgtttt ccttcatata aatggtcgta cagatagttg
     2341 cagatgatca gatccgctaa agccccctgt ctctttcttt ttcggatctc cgtcaataac
     2401 tttttcagag cttcctcatc cagccagtca tccgaatcca ccactttgaa atattctccg
     2461 gctgccagag acagtccttt gttcactccg gctccgtgac caccgttttt ctgatgtacc
     2521 gctctgactg tttccggata cttttctgca tagcaatctg cgatttcacc ggtccgatcc
     2581 gtggagccat catcgatcac gatgatctcc acctcctcgc ctcccgccat cagcgacctc
     2641 aggcatcgct gcatgtaatc ctgcgaattg tagcatggca ctgcaaa
//
LOCUS       DAWDWH010000248         2665 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_12919_length_2665_cov_10.916858, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000248 DAWDWH010000000
VERSION     DAWDWH010000248.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12919_length_2665_cov_10.916858"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1133..1142
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          833 a          490 c          710 g          622 t
ORIGIN      
        1 actgatcgag attctgcaga gacttgcaga aggcggaaat actgtagttg tgatcgaaca
       61 caatctggat gtgatcaaaa cagcggatta tatcatcgat atcggaccag aaggcggcga
      121 taaaggagga accgtgatcg cgaaaggaac gccggaagag gtggcgaaag ctgagaagtc
      181 ttataccgga aaatacgtga agaaatatct gtgaagattt tcttaatgtg caaatgaatg
      241 ttgtaaaaaa tagtttggta aaaataggac gccatgatga ttcgaaaaca atctcctgac
      301 acgcatcgtt tcctgttcgg ttcgctcgtg tgttagccgc ccttcgccta aactcgcctc
      361 ttcgaggctc agacagcggc tctctggggc ggcaacatcg caaacctcac gacggaagcc
      421 tcgctaccgt cagagtgatt tgttttcgaa ttcatcatgg ctgtattatt ttacgcacta
      481 tgttttacaa tgcggttttg tttgcacatt gccaaaagct actggcctgc ccggggtcgc
      541 ttaagcggaa agctttttca aaccacagat atttgctaaa aaactttccg tttccatagc
      601 gtaatcggct gggcaggagg tgtggaggct atttcttctt tcaacacaag aggagatgtg
      661 aagtgtagaa ttgtgccgga cagcaaaaaa gacagaggca cagtgacggc acaaatcgaa
      721 caggaaacga tgcgtgtcac gtgacctgtc tttttacgtc tggcactctg taacactttc
      781 tgttcattct tttgtactga aaaagaaaaa acggaacttg cgacataaaa atgaagaaga
      841 cacctgcggt gccttcttca aaggaaagag aaaaattaaa atattttgag ggagtactac
      901 gcaacgtgtg ggtgttgcgt agtgtgagtt atgaaatata ttagctcttc actaatacag
      961 ctacagtata gaatagaaat gtgttaaaag tatgtcttaa atattaataa aaaatgaaaa
     1021 gaagaaaaaa ataaaaaccc cttttcaaga agaaagccgt tgtatataat aaaccaagga
     1081 cagacagggg gaagatcctg atggagaaag gagaacacag gaatgaattc aannnnnnnn
     1141 nntggtgtac gtgaaaggaa aaggcgttgt tttaaaagag ccttccgtag tagcctttga
     1201 ccgtgataca aataaaatca aagcaatcgg agaggaggca agactgatgc ttgggcgtac
     1261 accaggcaat gtcgttgcaa ttcgcccgct gcgtaagggc gttatttctg attataccgt
     1321 gaccgaacag atgctgaagt attttatcca gaaagcgatc ggcagaatgt cttttcgcaa
     1381 accgaggatc agtgtctgtg tgccgagcag cattacagag gtcgagaaaa aggcggtgga
     1441 agatgcaacc tatcaggcag gtgcaagaga agtttctatt atagaggagc cgatcgcagc
     1501 agccatcgga gcagggattg atatttccag accatgcggc aatatgattg tggacatcgg
     1561 agggggtacc tgcgatgtcg ctgtgatctc tctggacgga attgttgtga gcacttcgat
     1621 caaagtcgcc ggtgatgatt ttgacgaggc aatcatccgt tatgtaagga aaaaacataa
     1681 tcttctgatc ggagaacgta cggcagaaga tatcaagatc aatattggaa ctgcgattga
     1741 acgtccggat cagaaagtaa tggaagtaag agggagagat cttgttaccg gcttgccgaa
     1801 aaccgttcgt gtgacttcag aggagacaag agatgcttta aaagagacaa cttcacagat
     1861 cgttgaggca gtacatggcg ttctggagcg tacgccgccg gaactggcag ccgatgttgt
     1921 ggatcgtggt atcgtgttga cggggggcgg ttcactgctt gggggcctgg aagagctgat
     1981 cgaagagaaa acaggcatta atacggtgac tgcagaagat ccgatgaccg cagtagcaat
     2041 cggcaccggg aaatttgtgg agctgacagc tggcaatggc aaactgatag agtaagggga
     2101 tttaagcaga aattcatgga taaaataaaa ggatatatag atcatctgat ttttcggaac
     2161 agtgacaatg gttacacggt tatggttgtg aaccacgaag gagaagaact aacctgtgtg
     2221 ggtaccttcc cggtgatcac agaaggggag ctgatcgaag cgaccgggca ctatactgaa
     2281 catgcagttt acgggacaca gttccagatc cagaaactgg aaataaaaga tccggaagat
     2341 acggcggcga tcgaacgtta tcttggttcc ggtgtgatca aagggatcgg aatcgcactg
     2401 gcagccagga ttgtgcggaa atttaaagaa gatacttttc gcattatgga agaagagccg
     2461 gaacgactgg cggaagtcaa aggaatcagt gagaaaaagg cccgtgagat ctcagaacag
     2521 gtatcagaaa aaagagatat gcgaaaagcg atgatctttt tgcagcaata tggtatttcc
     2581 aatacacttg gagccaagat ttataagcag tacggagcaa gactgtacgc tgtgattcag
     2641 gacaatccat atcagatggc agacg
//
LOCUS       DAWDWH010000249         2658 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_12959_length_2658_cov_11.305801, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000249 DAWDWH010000000
VERSION     DAWDWH010000249.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12959_length_2658_cov_11.305801"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1350..1359
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          837 a          478 c          684 g          649 t
ORIGIN      
        1 cctatgagca agctcatgtg gatttagacc ttttgaccct ggcatcatat catatgaaaa
       61 attgaagtgc atgaaagtag gtgtagatta tgtgtggaat ttgtggaata gcaggaaaat
      121 acgacaaaca gaagccggta atcgaaaata tgatgaaaag tatttatcac agaggtccgg
      181 acggcggcgg aaactatata gatgaggatg ttgcactggg atttcgaaga ctgagcatta
      241 tcgatctgga ctgcggcaat cagccgatgt tcaatgagac gggtgaagtt gtgatcgtat
      301 ttaacggtga aatttataat tatcaggaac ttacgaaaga actccaggca aaaggacatg
      361 tgtttgcaaa ccattctgat acagaatgcc tgatccatgc atatgaagaa taccacgaga
      421 aaatgctgaa aaagcttcgc ggaatgtttg ggtttgccat ctgggacagc aaagagaaaa
      481 cattatttgc agccagagac ttttttggca tcaaaccatt ttattatgcg gaaatcaatg
      541 gaaatctggt ttttgcatcc gagatcaaag caatcctgga atatccggag tataaacgtg
      601 agctgaatga aaaggctctg agccattatc tgagcttcca gtattctgct atggatgaga
      661 cttttttcaa aggcatctat aaattaaatc cgggatgctg tctgaaatac aaagacggca
      721 aattaaaagt ttcccgttat tttgatccga ctctggtacc ggagaagcat caggatgaga
      781 aagagatcgt agaagagatc aaggagcgtg ttgccgagtc gatcgaggca cataagatca
      841 gtgacgtaga agtaggttct ctgttatccg gtggtgtgga ttccaactac attgcagcac
      901 agggacattt ccagaataca tttacggtag gatttgcaga agacaacggc agatacagcg
      961 agatggagcg tgcaagagag attgcagatt atgcgggaag tacctgccat cagaaagtga
     1021 tcacaccgga agaatactgg gcagtgctgc cgaaagtgca gtatcatatg gatgagcctt
     1081 tagccgatcc gtccgcagtt gccctttggt ttgtagacga actggcagca agcaagctga
     1141 aggtagttat gtccggagaa ggtgcggacg agttgtttgg cggatatgtg atttatcatg
     1201 aaccagtctc tttacgtccg tttaaagtat tgccggcagg tctgaaaaag aagatcaaag
     1261 aaaaactgga agacaacaaa aagaatttta agggaaagaa ttatctgatc cgcggatgta
     1321 caccgattga ggagcgtttt atcggaaatn nnnnnnnnna tccaaaggaa aaagcaaaag
     1381 tcttaaagag cagcattccg gcagaagatc cgaagacact gacggcagcc tgttatcaga
     1441 aaaccagaaa actggatgat accgcacgta tgcagtatat tgacctgaaa ttctggctga
     1501 tcggagatat ccttcagaaa gcggataaga tgagtatggc acattctctg gagacgcgtg
     1561 ttccgtatct ggatattgaa gtttataaga cggccagaaa actttctttt gataataaaa
     1621 tccgcaagac acagacaaaa tatgccttcc gtgaagctgc acacagcatt cttccggagc
     1681 aggtggcaac acgcaaaaaa ctgggattcc cggtaccgac acgtgtatgg ctcaaagaaa
     1741 agaaatatta tgataaagta cgtgctgcat ttgaaagcga agcatcgaaa aaattcttcc
     1801 agacggatta cctgatcagg ctgatggatg atcattataa tggtctggca gataacagcc
     1861 gtaaaatctg ggcagtctat accttcctgg tatggtacga tgtctacttc ggtgagcagc
     1921 agagcgctta ataaaatggg agaactttac ttatgataaa gaaaaaaggt gcgatttttg
     1981 atatggacgg tacgcttctg gactctatgt gggtctggag gcagattgac gtggattttc
     2041 ttggcagaag aggatttgaa gtgccgtcgg attatctgga gatgatcaca ccgatggggt
     2101 attaccgggc tgccgagtat acgattgagc gattcggact gaatgaaaag ccggaagatc
     2161 tgatacagga atggcatggg atggcagcag aagcctatgc gaaaaaggtg gagctcaaat
     2221 cccatgcaaa agaatatctg ctgaaattga aaaaggccgg tacacgcatc gctgcggcga
     2281 cctcatcggc ttacgagctg attgcaccct gccttgagcg aaatggcgtg ctggagtgct
     2341 ttgatgcatt tgtgactacc atggaagtgc cacacggcaa agatttcccg gatgtttatc
     2401 tggaagcggc aaaaagactg aaactttcgc cggaagaatg ccttgtctat gaagatatct
     2461 ataaaggaat ctgtgcggcg aaaagtgcag ggtttaaagt tgtggccatg cgggaagaag
     2521 catctattga tgaccaggag aaaatccggc aggcatcaga ttattacata aaagattttg
     2581 tggaactcct taaataaaaa ttgaccgttg gtttcctggc aggtgtaaag aactgaaatg
     2641 ccgcacattt taaaatag
//
LOCUS       DAWDWH010000250         2619 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_13156_length_2619_cov_10.063963, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000250 DAWDWH010000000
VERSION     DAWDWH010000250.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13156_length_2619_cov_10.063963"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          725 a          552 c          613 g          729 t
ORIGIN      
        1 tcataccgaa gtatatgtgt atgaaagagg aatataaaaa tcagttagag tacataacgc
       61 atttctatat ggatctgtcc tggcgaagcc agtatgtagg tgtgcttcat atgatgtatg
      121 agggcatcca ttattatttc attgataatg aatactacct ggcaggacca agaccatatg
      181 gaaatattta cgaagatatc gagaagtttg cctttttctc aaaggcagca ctctcagcac
      241 tgccgctgat cggatttaaa ccggatatcg tacactgcca cgactggcag accggtctga
      301 tcccggtatt tttgaaggac aagttccacg agggcgagtt cttctgcaac atgaaatcga
      361 tcatgacgat ccacaacctg aaattccagg gtgtgtggga tatgaagacc gtaaaggata
      421 tcaccggact tccatcctac tattttacac cggacaaact ggaagcttac ggcgatgcca
      481 attacctgaa aggcggactg gtttacgcag atgcgatcac gaccgtcagc cgtacctatg
      541 cagaggagat caagacaccg ttctatggcg agcatcttga cggactgctc aatgcaaggg
      601 cacatgacct gcgtggtatt gtaaacggca ttgattatga tgaatacaat ccggagacgg
      661 atagatatat agtaaaaaat tataacgcaa agaatttccg caaagaaaaa gtaaagaata
      721 agagagcact tcaggaagag cttggtctgg aaaaagacga caagaagttt atgattggta
      781 tcgtatcacg actgacagac cagaaaggat tcgacctgat cgcctatatg atggatgaga
      841 tgtgccagca ggatgtccag tttgtgatcc ttggaaccgg cgaagagaag tatgagaata
      901 tgttccgcca tttcgcatgg aaatatcagg gcaaagtatc tgcaaacatc ttctattctg
      961 aaacgatgtc tcataagata tatgcatctt gcgatgcatt cctgatgccg tccctgtttg
     1021 agccgtgcgg tctcagccag ctgatgagtc ttcgttacgg cacactgcca atcgtgcgtg
     1081 agacaggcgg tctgaaagac actgtgatgc cgtacaatga gtatgaggga accggaacgg
     1141 gattcagctt tgcaaattat aatgcacatg agatgttcaa cacactgaag tatgcacagt
     1201 acatttatta tgagaaaaag cgtgagtgga acaagatggt agaccgtgca atggcagctg
     1261 atttctcctg ggcaagttcc gcaaaacagt acgaagaaat gtacaactgg atgcttggat
     1321 gctgaacaaa aaggatgtaa gagattggaa aacgatctta agaatcaaat aaaagaaaag
     1381 cttcttcgaa acgaaataga tcgaagaagc ttttttatgc ctttcttctg ttttaatcag
     1441 agaggtgcct tttggaatgt ttcttttttg gaatcagttt ggaccttgtc ttggaacgtt
     1501 ttacaaatct tccgtccgca ccatagcggt aataatattt gccgatcttt acgatcttat
     1561 ttttgaccat atgaccgctc ttatcgacgt aatactcgcc aagctgggca tttgttgcca
     1621 tgtatccggt cgaataaaag tagtaccagt atcctttgat gctctgccaa gtctttgcag
     1681 agtaggtgcc atccacattc cggtattttg tgccctgggc ggtcttgata aatttcgctt
     1741 tgtatttttt gccgactgcc acgcgggaac cgtttttgtt gacgtagaca ttgtggttgt
     1801 ccacccagcg gcttctggcc atcttgccat ttttcatcag gtatttgtca ccgatccact
     1861 ggcttgtcag catggcaccg ctctccgaga agaagtagcg atcgcctttt aaatccagcc
     1921 atccggtgtg gaccgttccg tcggcattaa aatagtaagt atagcctttg atcttacgaa
     1981 atacagaagt aagataggtg ccgtcttttg tcttgtaacg cagtgtattt tcttctgcat
     2041 tgtactccca gtcggcgtgg acggtgtatg acggaatcag cagagccgca cagaggaccg
     2101 ggatgatgca ggtatgtttt aaattgtgaa gtttcaaaaa aacccctcct tgtaaaattc
     2161 agaaattttt ttcagccgaa gaagactccc acttcctgac tgcaacaggt tattactaca
     2221 acagtatccc atagaatccg aattctgaca aggaagagtt tttaaaatta agaaaaactt
     2281 atgacagacg catgtttcct aaccctggga aaggacggag cgaacgtgct ggatttcttc
     2341 ggcggtcgcc tttgcggccg gtacatagta ctgtttgctg cgggcaagct gatagatctc
     2401 ttcctgagaa gtttcacact ggttgcggaa ctgctttaaa acctgtttca gctgcatatt
     2461 ttcggtctgt gggatcattt cgccatagcg gaccagttct ccgttgatgc cggctaatgt
     2521 gtcggctacc attgtctttt catctaacat cttttcttac ctcctgttac tgcagaaatg
     2581 tcatcagctg ctgtttgcac tgcatcgcgg actgtgcag
//
LOCUS       DAWDWH010000251         2600 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_13258_length_2600_cov_8.110020, whole genome shotgun sequence.
ACCESSION   DAWDWH010000251 DAWDWH010000000
VERSION     DAWDWH010000251.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13258_length_2600_cov_8.110020"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    995..1004
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1786..1795
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          579 a          675 c          501 g          825 t
ORIGIN      
        1 tttaattctt ctttgccgga aacagaaact tctgtttttc catctgcccc ggatacagtc
       61 acatcgcttg gaacttccgg tcttgtaatt tcaagtgtcc agctgacatc cagtcgaaat
      121 gtttctttgc tgtctgaaac aatctgaata taataatcca cgtccttgtt ccagcagata
      181 ctgacgctcg tacttccctg aacctgatgt gccacatctc cgatctgccc tgccagttcg
      241 tatccgccat ctgctttctt ccagactgct acatagtagt ctgtgaaata atcctccgaa
      301 ttgctcacta acaagtcttt ccatacatct ttcgtcggct gataatgata ccagccggcc
      361 ggaaccctcc agtaagcacc atcataatat gctctggttt cttttaccgt ttccgtaccc
      421 cacggagccg cactctgtat ctgtgcggat gctgacaact gtgtctgtgc agataccggc
      481 agaatattaa cccacaccag aaacaggcac aatgggagca ggcagcatat gatccgccgg
      541 aactgttttt ctcttttctc ttccataaaa ttctttcctt tccttttttc ttcattatag
      601 caaggccatc tgccattctc aacaaaattt tatcgttttg cccggtattt tttacggaga
      661 tatcgacctg cctatgacag gtcttttttc ttccagtttc ctgttttact gcttcccgga
      721 gccaataagg cactgaactc gtttttgttt gtcaaactcc agcagaaata gctcacattt
      781 gtctgatcca gccttttcag ccatttcccg gtatttatcg tactgatctg accattgcca
      841 ctcgcatccg acatgctcat tatggctcca ttcatttgca taaaaatgca gagaatacat
      901 gatattttta taacccttga tcggactgtc cgccacttcg ttgtgcgttc cgtccgatcc
      961 aagctgcgac catgtcggcg tgcctgtttt tacannnnnn nnnngattaa aagtgatcgt
     1021 tgaaggtgat agttgtaata ttaaaaaacg cccccaaaca caattcaatt agtaatactt
     1081 accaattttc attgcattta gaggcgttta atcacttctc gcttggacac attagccaaa
     1141 aatagctatt tatttgtagt taacgatctg accgaactct tctttcagag atgctcctcc
     1201 aaccagtccg ccatcgatgt ctgcctgagc aaacagatct gctgctgtct tggagtttac
     1261 agatccgccg tactggatac ggatctctgc tgctgtagcg tcatcgtata cttcagcgat
     1321 acactcacgg atcttgctgc atacttcctg agcctgctct gttgtagcag tcttgccggt
     1381 tccgatagcc cagatcggct cgtaagcgat aacggccgct tttgcctgat ctgctgtcag
     1441 tccctggaat ccgatcttaa cctgctgacg gatgaagtcc atggtcacgc cctgctctct
     1501 ctgttccaga gattcgccgc agcacatgat cggtgtcagg ccatgctcaa atgctttgtg
     1561 catttttttg ttgatatctg catctgtttc tccgaagtat cctcttctct cggaatgtcc
     1621 cagaacaacg tatttaacgc ctgcatctac cagcatattc ggggagattt cacctgtgta
     1681 ggcacctttc tcttcgaagt acatattttc agcaccaacc tggatgtttg ttccttttgc
     1741 agcttctact acaggaacga tatcgatagc cggtacgcag aatacnnnnn nnnnnaatac
     1801 tacatcaact tcatcatttt ttaccagtgg tttcagagtt tcaactaatt ttactgcctc
     1861 actcggagtc atgttcattt tccagttacc agcaataatt ttctttcttg ccatgatctt
     1921 atcctctttt cacatattgt aaaacatcgc ctttggctcg tttaagtaaa cactagaact
     1981 caaacatcgc cttcggctcg ttttcgttaa gtgtttactt atcgttagct gctgctacgc
     2041 ccggcagttc ttttccttcc aggaattcca gagatgctcc accgcctgtg gagatgtggc
     2101 tcatcttgtc accaaatcca agctggttta ctgctgctgc agaatcgccg ccgccgatga
     2161 tggttgttgc atcggtatct gccagagctt ttgctactgc gatcgtacct gctgccagtg
     2221 tcgggttttc gaatacaccc atcggtccgt tccaaacaac tgtttttgct gtttttactg
     2281 catctgcata cagagctgct gtttcttttc cgatatccat agcttctttg tcaaccggga
     2341 tattatctac agatacattc tctacttcga tcggagcatc gatcgggttc gggaaatctg
     2401 cggaaacaac tgcatctacc ggaagcagaa gttttttgcc aagcttctca gcttttgcca
     2461 tcatctcatt gcagtagtcg atcttgctgt cgtctaccag agatttacca atctctttgc
     2521 cgtgagctgt ctggtaggta ttggccattc agccgcgaat gatcagtgtc tggcatttct
     2581 cagggagggt ggagagaggg
//
LOCUS       DAWDWH010000252         2568 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_13409_length_2568_cov_11.989654, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000252 DAWDWH010000000
VERSION     DAWDWH010000252.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13409_length_2568_cov_11.989654"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1063..1072
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          756 a          501 c          685 g          616 t
ORIGIN      
        1 gtaacattta atccttgtct ggattatatc gtaggtgtgg aagattttca actgggaatg
       61 acaaaccgca cctgttcaga gcagatgttt gccggtggaa aggggatcaa cgtctctatc
      121 gttctgaaaa atctcggcat tgagagtacg gcacttggat ttatcgccgg tttcaccgga
      181 gaggagatcc gcagaaaagt cgagaagatg ggcattacat ccgaatttat ccagcttgag
      241 aatggatttt cccggatcaa catcaaactc aaatccatcg atggaacgga gatcaacggc
      301 acaggacctt cgatcggaag tgaagaactt gaaagattgc ttaagaaact ggatacactg
      361 aaaaagggag atgtgctgat ccttgcaggc agcatcccgg caagtcttcc ggattctatt
      421 tacagtgaca ttatggaacg tctggaagaa aaagaggtca tgatcgtggt ggatgcgaca
      481 aaagaccttc tggtcaatgt actgaaatac catccgttcc tcatcaaacc aaacaatcat
      541 gaactgggcg atatctttgg agtggagatc agagagcgtg accaggtagc accatatgcg
      601 aagaagttac aggagatggg agcaagaaat gttctggttt ccatggcagg caaaggtgca
      661 gtgcttcttg ctgaggatgg cagcgtatat gaaagcccgg caccgaaagg taaactgatc
      721 aatgcggttg gagccggaga ttccatggtg gctggtttta ctgcaggtta tatgcagaaa
      781 ggcagctatg aacatgcatt caaaatgggc gtttccaccg gaagtgccag tgcattttct
      841 gagaattttg ccacaaaaga agaagtagaa gcgatttata aaacattgta aagatgccag
      901 ggtcaaaatc ctgaaactat gatgtcgaag tgaggaacaa ttcgtatggc atttttgaat
      961 tagcttcttg tagaaaatct atcacaaaaa agaaatgtaa gaaaggagaa cttatgagga
     1021 tcacagaact gcaggataag cgaagcatca ggatcgacgg tgnnnnnnnn nnctctcgac
     1081 cagatggtcg aactgatggc aaagagtgga aaatcaatga cctggaagct tatcgccagg
     1141 aagtataccg ccgtgaagag gaaggaacga caggtgtcgg agaggggatt gcgatccctc
     1201 acggaaaagg tgcatttgta gataagccgg gactggcagc gatggttgta aaggacggtg
     1261 tagattacga ttctcttgat ggagagccgg tacatctgat ctttctgatt gcagcaccga
     1321 ataccaaaga caacgtacat ctggacgtac tgagcaaact ttccgtactt ctgatggatg
     1381 aggatttttc cagagcactg cagaatgcaa aaagcccgga agaatttatg aagatcgtcg
     1441 atgaagcaga tcaggaagca ccggatctgg atgaaaaact gaaagcagac gaagcgacct
     1501 cggaagatca gttccgtatt ctggctgtca cagcatgccc gaccggtatc gcacatactt
     1561 acatggcagc ggaaggtctg gaaaaggctg caaaagcgaa aggatgtttc attaaagtag
     1621 agacaagagg ttctggcgga gcgaaaaacg tgctgactgc ggaagagatc aaaaatgcag
     1681 actgcatcat cgtggcagca gacacaaaag ttccgatgga tcgattcaat ggtaaaaagc
     1741 tgatcgaagt tcaggtttct gacggtatca gcaaagcgga taaactgata gagcaggcaa
     1801 tgtccggaaa tgtaccggtt tatactgcat cggaaaatgc agaagtttcc gagaaaaaag
     1861 caggaggctc caaaggccat cagatttata tgcagttgat gaatggtgtt tcacacatgc
     1921 tgccattcgt cgtaggcggc ggtattttga tcgctcttgc attcctgatc gatggacttt
     1981 gtgtagatat gacatcattg acagcagaag cacgacagag tttcggttcg atcacaccgg
     2041 tggcggcaac actgaaatcc ataggaaatc ttgcatttgg atttatgctt ccggttctgg
     2101 caggatttat tgctatgagc attgcagaca gacctggtct ggcagtcgga tttgtgggtg
     2161 gtgcaattgc agcaaacggc aagtctggtt tcctcggtgc attagtagca ggttttctgg
     2221 caggctatct tatccttctc ttaaaaaaat tgttcggaaa acttccggaa gcactggaaa
     2281 aaattgctcc agtccttctc tatccggtat tcggcattct gatcgtggga cttgttatga
     2341 cgtttgtcgt agagcctcca atcggagcat tgaacacggc actgaacaca gctcttacaa
     2401 acatgagtgg ttccagtaag gttcttcttg gcatcatcgt agcaggtatg atggcagttg
     2461 acatgggtgg cccgttcaac aaagcggcct atgtatttgg aacggcgtcc atcgcagcag
     2521 gaaattacgg aatcatggca gcagttatga tcggcggtat ggttcctc
//
LOCUS       DAWDWH010000253         2561 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_13451_length_2561_cov_8.129689, whole genome shotgun sequence.
ACCESSION   DAWDWH010000253 DAWDWH010000000
VERSION     DAWDWH010000253.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13451_length_2561_cov_8.129689"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          762 a          568 c          494 g          737 t
ORIGIN      
        1 gtatgatttt tctcatataa tatccctttt ctactgtttt ctgtttgtgt aaatcaaagc
       61 atttacttct gtaacagtat accctgtcac ctaacatcgt gcaaccgtga aaatgtaaaa
      121 aaagtcggca ccaattccca tatctgcaag gaatatatgc caactttcat ttttgcaaaa
      181 aaataaagct gctgacggga atcgaacccg tgacctccgc cttaccaagg cgacgctcta
      241 ccgactgagc cacagcagca tttccataag cggacatttc tgtcgctcac agttgataat
      301 attaacagaa aaagcatacc ctgtcaacag gcaatttgat ttttttgcct ttgtgttttt
      361 ttacatttat ttctcgatat ttttctcttg aaatctttta aatccggcgt ttttatgata
      421 tgattaacct ttaaagaaac attcaggagg gaaagagatg gaaaacgaaa ccgtttctaa
      481 aaattttatt gaaaacatca ttgataaaga ccttgcagag ggtgtttatg ataccgtaca
      541 cacccgcttc ccaccggagc ctaatggata cctccatatc ggacatgcca aatccattct
      601 tttgaattac ggacttgccc aggaatacaa cggcaagttt aatatgcgtt ttgatgatac
      661 aaaccctaca aaagaaaaaa gtgaatttgt agaatctatt aaagctgata tcaaatggct
      721 tggtgctgac tgggaggaca gacttttctt tgcttctgat tactttggac agatgtatga
      781 agcagccatc aaactgatca aaaaaggaaa agcttatgtc tgtgacctga ctgccgatga
      841 gatccgccag taccgtggaa ctttgacaga gcctggaaaa gagagcccgt accgtaaccg
      901 ttctgttgaa gaaaatctcc agctttttga agaaatgaaa gaaggcaaat atgccgatgg
      961 agaaaaagtt cttcgtgcca agatcgatat ggcatctcct aacatgaaca tgcgtgatcc
     1021 ggtcatctac cgtgttgctc atatgaccca ccacagaacc ggcgacacct ggtgcattta
     1081 tccaatgtat gactttgctc atccgatcga ggatgctatc gagggaatca cacactccat
     1141 ctgtacactg gaattcgaag accatcgtcc gctttatgac tgggtcgttc gtgagctgga
     1201 atacccacat ccgccgaaac agattgaatt tgcgaaactg tatctgacca acgtcgtaac
     1261 aggtaaacgt tatattaaga aactggttga agacggaatc gtagacggat gggatgatcc
     1321 acgtcttgtt tccatcgcag ctcttcgcag aagaggcttt acaccggaat ccatcaaaat
     1381 gttcgtagat ctctgcggtg tctccaaagc aaacagctcc gtagactatg caatgcttga
     1441 gtactgtatc cgtgaagacc tgaaattaaa acgccctcgt atgatggcag ttatggatcc
     1501 aatcaagctc gtgatcgaca actatccgga aggacaggtt gaatatctcg atgctccgaa
     1561 taacatggaa aacgaagcac ttggaacccg taagattcca ttcagcggtg aattatatat
     1621 tgaacgcgat gacttcatga tcgatccacc acgtaaatat aagagacttt tcgttggtac
     1681 agaagtacgt ctgatgaatg cttattttgt aacctgtaca ggatatgaag cagatgatga
     1741 cggtacagta cgagttgtac attgtactta tgatccggca acaaaaggcg gaaacgctcc
     1801 ggacggacgt aaagtaaaag gaacgattca ctgggtagaa gctacaaatg ccaaagaagc
     1861 aactgtacgt ctttacgaaa acattgtaga cgaagaaaaa ggtgtataca acaaagaaga
     1921 tggttctctg aacgtaaatc caaactctct tacaactgtc actgcttatg ttgagccggc
     1981 tcttatggaa gcaaaaggct atgacagttt ccagtttgta agaaacggat tctactgtgc
     2041 agatattcat gattctaaag aaggtgctcc ggtatttaac cgtatcgtat ctctgaagag
     2101 ttctttcaga cttcctaaat aaacacaact atttcaagag aggcagacca taaaggtctg
     2161 tctctctttt taacataaaa aactgccgtc ctacaaatgc agtgcattca taaaacagca
     2221 gttctactta tccatgaagg attatttatt cctcttctgt atcttcgtct tctttttctt
     2281 ctttgatcac ttcttcaaca gcctcttctg catctgctgt atcagcagtt tcttctactg
     2341 ctttctctac agcttcttct gcttcatccc aggatgcaaa atcttcgctt gtttctttct
     2401 tttcttcttt ctcagcagtt tcttcttcgc ctgtctcatc ttcgaaatca tcatcgaagt
     2461 catcaaaatc atctaacgga tctttcttca gtacaagtgc tgcgataact cctgccgcag
     2521 cggcagctac cgctccaact gctacatatc cccaatattt a
//
LOCUS       DAWDWH010000254         2446 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_14072_length_2446_cov_7.775826, whole genome shotgun sequence.
ACCESSION   DAWDWH010000254 DAWDWH010000000
VERSION     DAWDWH010000254.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14072_length_2446_cov_7.775826"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          738 a          498 c          550 g          660 t
ORIGIN      
        1 gagctgacga gatcgctgta tggctgctgc gtacattcat ggataaactg aaatactgcc
       61 atacctaccg tgattctgag ccgacaactt ctatcctgac aatcacatca aacgtagtat
      121 acggtaaagc aaccggttct ctgccagatg gaagaaaagc aggcgagcct ctggcacctg
      181 gagcaaaccc atcttacgga gcagaacaga acggtctgct ggcatctttg aactctgttg
      241 caaaacttcc gtacgaagat gcactggatg gtatctccaa tacacagacc atcaacccgg
      301 gtgctctggg acacgatgat gcagagcgta ccgaaaatct ggttcatgta ctggatggtt
      361 acttcaaaca gggtgcacat cacctgaacg taaacgtatt cggaaaagaa aaactgatcg
      421 atgctatgga gcatccggaa aaagaagaat acgcaaactt cacaatccgt gtatcaggat
      481 atgctgttaa atttatcgac ctgacaagag agcagcagct tgacgttatc gcaagaacct
      541 gccacgacag aatgtaatat ggaaaacggg gcaaaaacag gatatgtcca ttcgctggaa
      601 agctttggat cggttgacgg tccgggtgtg cgttatgtga tcttcttaag cggctgtgcg
      661 atgcgatgcc agttctgtca taatccggat acctggaata tgacagccgg caaaagctac
      721 acagctgatg cccttctgaa acaggcagta aaataccgca cttattgggg aaataagggc
      781 ggtattactg tcagtggagg agaaccactg cttcagattg attttctgat cgaactgttt
      841 cgcaaggcaa aagagatggg gatccacaca acgctggata cgagcggcaa tccttacacg
      901 gaaaaagagc cattctattc aaaatggctg gaactgatga aatacaccga tctgattctt
      961 ctggatatca agcatattga tgaacaggag catattaaac tgaccggtca gaccaacaaa
     1021 aatattctgg ctatggcaga aaagttatcg gatatgggaa aaccggtatg gatccgtcat
     1081 gtactggttc cggagcggag cgaccgtgat gattacttgc accggctggc agattttatt
     1141 cacacgctga aaaatgtgga gcgtgtggaa gtactgccat accatacgct gggcgtgttc
     1201 aaatgggaaa atttaggaat tccatatcag cttgaaggga tcaatccgcc aagtgcagaa
     1261 cggatcaaga atgcagaaga gattctggcg ttcaggaaat agaaataaca ggaaaatttt
     1321 aaatgtgcaa aggaacaatg tcctgaagga cacataaaag tgtcttcctg ggcattgttc
     1381 cttttgcaca tttggaaagc ggacattcac aatccgcttc tctgcgttca aatttcaata
     1441 tttcacgagt tttttaaagc agagaaaatt tactccacaa ccggaggtac aaaatgttcg
     1501 atcgcatttc gcagtttcgg atgattagcg gatggtaacg ctgcgttttc ggctgttata
     1561 cgtatagact ttttcatcca gatcttttaa aacctttata tttcttttta cagtatctcg
     1621 ttttctaaga aaagagggag catcgataaa agtgaccttt gcatggatat atttgaaaaa
     1681 ggattctctc ttgtcgttgt ttccatgatg cggagcctga agatacgttg ccttcagacg
     1741 tttgtgataa gttttgataa gtcttttgcc aatgctgctc acaatatctc cggtgaaaag
     1801 catacttgtt tttttaccgg aaattttaaa aaccagagaa gaaccattgc agatatcttt
     1861 ggtcgtatct ttcagattac ggctgtaacc attaaaaaat tcaaaatcca gaccggcgaa
     1921 ggaaaaagta tcatctgctt ttgcccaccg gatcttggga ttttttctta caagacgtga
     1981 aaacttctga aatacctgat attcatccca tggatattga tagtgtctgt aacgggaagc
     2041 gttgatcttt ggtgcaataa cctgatcaat ctgtatgccg tccggattgg caaaaatctg
     2101 attgaaagct ccgacatgat ccggatgcgg atgtgtgata atccagagat ttacctttcc
     2161 gcccagtttc tttatggtat cgcgtactct tttcgcatca taatcccatc caccgtcaat
     2221 tacgatcagt ttgccgttgc ttcctttgat cgtataaaac atggactgct gaaaatcaga
     2281 atcagatcca tattgtgtga ctttccagga agatgcataa actttttctt cgcagaacgg
     2341 atgcaggctg aagaataaaa aaagtccgac agacaagaaa aaccgaaaag tatataaatg
     2401 aaaaagctgt gttttatttt tcattgggat ctctccttta gtgata
//
LOCUS       DAWDWH010000255         2420 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_14230_length_2420_cov_7.734884, whole genome shotgun sequence.
ACCESSION   DAWDWH010000255 DAWDWH010000000
VERSION     DAWDWH010000255.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14230_length_2420_cov_7.734884"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    940..949
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          763 a          454 c          641 g          552 t
ORIGIN      
        1 tggatatttt gcacaggaag tgccggatat gaataccgag cagcgggtga tcgattacat
       61 caaagatgtg gcagagtatc tgccgacaaa agacgggaag atctcggcat ctcagatgct
      121 ggagcgattt ctgtttacac cggaaatgca gtatgcaccg gtatccaagc tttctggcgg
      181 ggagaaaaaa agactgtacc tgctgaaagt tatttttacc ggggcaaatg tatatctgtt
      241 tgatgagctc tccaatgata ttgatattca gacgctgacg attctggagg atttcctgaa
      301 cagttttccg gggattgtga tcaccgtatc ccatgaccgc tattttctgg acaatgtggt
      361 ggaccgcatt tttgaatttg acggaaacgg tcatttgcag cagtacgaag gtggttatac
      421 cgactatctg gaaacaaaac agaaaagaac atcacaagaa acatcggaaa gcgggaatgc
      481 acttttcggg aaatccggaa agggaaagtc tgaggaaggg agttcaaaat cttccagaga
      541 ctggaagcag aaccgtaaga caaaactgaa attttcctat aaagaacaga aagaatttga
      601 aacgatcgat gacgacatcg cacagttaga agatgggatc gccgctctgg aagctgagat
      661 tctggcaaat gcaacaaatc cgggaaaatt aaacgaactg atgaaagaaa aagaaaaggc
      721 ggagcatgca ctggaagaaa agatgaatcg atgggtttat ttaaatgatc tggcggaaaa
      781 aatagagtca cagaaaggtt gatgaaagtg gaaacattcc aggcaatggg agccgttccg
      841 gtcaaagacg gaatcgtctt ttcgatggaa cgcaaaaaca acgaagaagc cagcctgctt
      901 ctttataaaa aaggcagtaa agaagtaata caggaaatan nnnnnnnnnc tgtgtcaaag
      961 cagaaaaaat agcatctgca cgatatgaat acaatttttg catagatgga aaagtaactt
     1021 tggatcctta tgcaaaagta ctgatcggaa ccgggaaatt tggtgaggag catccggagg
     1081 gacacgaagt ccgctgtgcg atgatcgccg gaaattacga ctgggaggat gaccggaaac
     1141 cgcagatcgc atacgaagat gccgtgatgt acagctttca tgtacgtggg tttacaaaac
     1201 agcgttattc cggtgtgcgt cataagggaa ccttccttgg aatcaccgag aaggcggaat
     1261 atttcaaaga gcttggcatc aatcagatca agctgatgcc ggcctatgaa ttcactgaaa
     1321 tggagtctgt gaaaacacat gcccggtacc gtaaagaaga agagcttccg aagcgtctga
     1381 actactgggg ctatacaaag gcctttcatt ttgccccgaa acgtgcctat gcagcgacaa
     1441 aagatccggt gcgtgaattt aaagatatgg taaagaccat gcaccgtctt ggtatcgaag
     1501 ttctgatgga attttatttt ccggaaggat gcagcccgcg ttatattacg gaatgtctgc
     1561 aatactgggt gcaggaatat catgtggatg gatttcatgt ccgcggcgtg cagggcatct
     1621 gtaacctgat ggcgacggat ccactgtttg cagataccaa gcttttaaat atttatttcc
     1681 cggtggagga gatctatggc aagaagaatc tgccaaaaaa acggatggtg gcagagtgca
     1741 acgatgggtt tatgatcgat gtccggcgtt tcttaaaagg agacgaagag agcctgaaag
     1801 catttgcaga gcggatgagg cggaatccaa agggcagcgg gctgatcaat tacatagcaa
     1861 gccatgacgg gttcacatta tgtgacctgg tatcttatga agaacgtcac aatgaagata
     1921 acggcgagca gaaccgtgac ggaagagtgc agaattacag ctggaactgc ggagaagaag
     1981 gaaaaagccg caaaaagaaa attctggagc ttcgaaaccg tcagatgaaa aatgcatggt
     2041 gtatgctgct tttgtcggca ggaacgccga tgatccttgc cggggatgag ttctgcaact
     2101 cgcagcttgg caacaacaat ccatattgtc tggacaatga gatttcctgg cttgactgga
     2161 aaggatataa atccggaaac agtgaaatgt tccgctttgt aaaagatctg attgcatttc
     2221 gcaaaaagca taaaatcctg catatgggac aggaactgag catgacggac agcttatcct
     2281 gcggttttcc agggatttcc tatcacagca gcagtgcgtg gtacggggaa ctggacgggc
     2341 agaaccgtaa gatcggtgtc atgtactgcg gcaaatatgc cgatgaggat gaactgatct
     2401 atattgccta taatatgcac
//
LOCUS       DAWDWH010000256         2389 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_14434_length_2389_cov_6.546272, whole genome shotgun sequence.
ACCESSION   DAWDWH010000256 DAWDWH010000000
VERSION     DAWDWH010000256.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14434_length_2389_cov_6.546272"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1948..1957
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          591 a          572 c          426 g          790 t
ORIGIN      
        1 tttattttac attgaatttt ctgtcttttt aatcgctaaa tatctctgta ttgcattttt
       61 attgaaacac ttttataatg atgccagaat tcaaaatcaa cttcagaaat gccggaggca
      121 ttcttgaaca cataagttta agaaaggaat tttttcatgc tatataaaca acaatctttt
      181 tccaactttg aaccagatct tcgctatctg gactcttttc ctttttccag agaagaattt
      241 ctcttctttg acattgaaac aaccggattt tccccgcgaa acacttctct gtatctgatc
      301 ggtgcggtct gctttctgga tgagcactgg actttccttc agtggtttgc cgaaactcca
      361 tcggaagaat gtgaaatttt aaacgcattt cttgcattct gccggtcatt tcgtgctgtc
      421 gttcatttca acggtgacgg ttttgatatc ccttatcttc ttttcaaagc agaaaaatat
      481 acattgtcag ataccttatc atctatgcag agcattgacc tttaccgcct gacccggcca
      541 tacaaaaaat atctgaaact gacgcacatc aaccagaaaa gtctggaatc tttccttggc
      601 attgagcggg aagacctcta caacggcggc gaactgatca aaatctatca gagctactgc
      661 cagttaaaag atgcggatgc tctttcgctt ctgcttctgc ataatctgga cgatctgaaa
      721 ggtatgacaa aactgctgcc gcttcttgcc tatccgctgt ttttgaaaga ccaggattat
      781 acgctccgga atcttttctg ggaagattct gtgaacggac agccgcttct gatcgcagag
      841 ctggatctga aatatcctgt tccaaagcag atctccaacc ggctttcctc gtgctatctt
      901 acgctccagg aaggcactgc caaactgatg atctatgctt tcgagggcga attaaagtat
      961 ttcatatctg actataaaaa ttattattat attcctttgc aggactgtgc cattcataag
     1021 cacgttgcct cttatctcga tccacgtgaa cgcatccctg caaaagccac aacctgttac
     1081 cagcgtcacc ggggactttt tcttcctcag gaaaaagcac tgttcaaacc tgttttccag
     1141 gaaagctatc attcggattc tttctggttt gaatgtacgc aacagtttat gaattcgccc
     1201 tctttattat gtttatacat aaaaagcctg atttccattt gaaatcaggc tttcctgccc
     1261 tgcaggttct cttttgtctg aaccttacag gacactcagc actccctttt tgagatgtgc
     1321 aatattactc ttcttctgcc tctgttgtgt cggcagcagc ttcttctttc tgcagtgctt
     1381 tcatgctcag gctgattttt ttgtcttcgc cgttgaaatc tactactttt gctgtgataa
     1441 cctgtcctac gctcagtacg tcagatggtt tatcaacatg ctcctgggaa atctgtgata
     1501 catgaagcag tgcgtctaca cccggctcca gttcaacaaa tgcaccaaaa tcagtcattc
     1561 ttgcaacttt accttctacc acgttgcctg ctgcgtattt ctcgccagct gtcagccatg
     1621 gattctgatc cgggaatttc aggctcagtg caatcttatc gccgctgatg tctttgatca
     1681 gaacagaaag tttttcgcca actttgaata ctttctttgg attttcaaca cgtccccagg
     1741 acatctctga gatgtgcagc agaccgtctg ctccgcccag gtcgatgaat gcaccgaagt
     1801 ctgtaacgtt tttgataacg ccttctacta catcaccaac gtggattctt gcaaagagtt
     1861 ctttctgcat ttctgctttc tttgcaacca acagctgttt acggtcgccg atgattcttc
     1921 ttctgcgtgg attgaactcg ctgatcannn nnnnnnncga tgaccgtacc ttcgactacc
     1981 tctccattat gaattgtttt taatgattcc tccagcattt gttcaaagct catttctgac
     2041 attccttgtg aacctcctca ataatattat ttggggttga tgcccctgct gtaataccta
     2101 cgcaactcat ggactgcatc gaatgtacat ccaaatcttc aagtgtctgt atatagtaag
     2161 tattcttgca ttcttcttta catatttcaa atagtttctg cgtattggaa ctgtgtctgc
     2221 ctccaattac gatcatggca tccacatcgg cggcaatttt tctcgcctcc tgctgacgct
     2281 cttcggtcgc attgcaaatc gtatttaaaa caagtatatc ataacgcttt tttgaaagaa
     2341 tttcaactaa tttattaaat ttcttgtaat taaatgtcgt ctgagacac
//
LOCUS       DAWDWH010000257         2352 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_14606_length_2352_cov_9.861559, whole genome shotgun sequence.
ACCESSION   DAWDWH010000257 DAWDWH010000000
VERSION     DAWDWH010000257.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14606_length_2352_cov_9.861559"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          723 a          440 c          661 g          528 t
ORIGIN      
        1 ccgacgctgt tttcttaccg ggaacgtata caggtaaatg aaatatatat aaaaaaagat
       61 gctctggcaa ggctgacgac gcagatacaa agctgtgccg atgagatggt acgtgacggt
      121 gcacctcatc cgaccgcttt tgagatcgag acggcacttt gctttctcta tttcagggag
      181 gaaaagtgtg atctggtcgt tctggaggtc ggcatgggag gagctaccga tgccacgaat
      241 gtgataaaga cgaccgttct ggaagtgctc gcatcgatca gcatggatca tatgggattt
      301 cttggagaca ctctggcaga gatcgccgcg tgcaaagccg gtatcatcaa gccggatacg
      361 actgtggtca gcatgagaca gcagccggaa gccatggaag tgatccggca ggtctgtcaa
      421 aggcagaagg cggatctgcg tatcgcagat gcggcaaatg caacggacat cgtatatggg
      481 tgtgaagagc agtatttttc ctatggcggg tatgacagcc ttgagatcca tctggcaggc
      541 tcttatcaga ttgcaaatgc cgttctggca gtggaagcgg taaaagccct gatcgatctt
      601 ggctatgcca tttcagaaga agcactgcgg agtggtctgc aaaaagcaag ctggaagggc
      661 agatttacgg ttcttcaaag agagccatat tttattatag acggagcaca taaccgcgat
      721 ggggcaaagg tactggctga ttcagtacgt cagtattttc cgaaaaaaag gctccattac
      781 attatgggtg tttttaaaga taaagaatac gaaacgatca ttgatcagat gtctgtattt
      841 gatgcggatg tgatcacggt agagacaccg ggcaatccgc gtgcactgcc tgcggaagaa
      901 ctggcagaag catggaaaaa gaaaaaagcg gatgtgacat ttgagaaaaa gatagaaaat
      961 gcggtaagaa aaagtctgga aaaggcgaaa aaagacgacg taatcctcgc tttcggatcc
     1021 ctctcgtttc ttggtgagat tgtgaaagct ctggatacaa taagaagtga ggtttgaaaa
     1081 ctatggtaga tcaggaaaaa gtaagagaag gggtacgact gatactggaa ggtatcgggg
     1141 aagatataaa cagagaggga ctgatagaga caccggaccg tattgcgaga atgtactgtg
     1201 agctggcagg aggcatggat cagaacgcag cgactcatct ggagaagcgt ttccatgtgg
     1261 acaatgcgga tatggttgtt gagaaggata tcacatttta ttcgatgtgc gagcatcaca
     1321 tgcttccgtt ttttggaaaa gcacatgttg catatattcc aaacggcgag gtagtcggtc
     1381 tgagcaagat cgcaagaaca gtagaggttt ttgcaagaag actgcagctc caggaacagc
     1441 tgacggcaca gatcgccgat gcgtttatgg acaatctgca cccgcagggc gttatggtta
     1501 tgatcgaggc agagcatctc tgcatgacga tgcgtgggat caaaaaaccg ggtgcacaga
     1561 cagtaacgac cgtgacacga ggtgtgtttg ataacaacaa agaactgcag gatacttttt
     1621 atcagatggt gcgtttataa tgagaatgga acgtgtaaat cagattttgc agcacacact
     1681 ttatcagagc tgccgcagac agattgatga aaaagaaaaa gaccggattt tctgtggaca
     1741 tgatatggga catctgctgg atgttgccag actggcatgg atctttaatc tggaagcaaa
     1801 tcaggagata tcgaaagagc ggatctatgc agccgctctt cttcacgata tagggaggca
     1861 tattcagtac gaaaacggaa ctccgcacca gatcgcaggg cttccgatcg cagagcagat
     1921 cctgacggac tgcggatttt atccggaaga gatgaaagag attctctatg cgatcgaaaa
     1981 tcaccgcaac aaagagatgt taaagcggga tgatcttgcc gggatcttat accgggcaga
     2041 taaaatgtcc agatgctgct ttggctgccg tgccgaaaaa gagtgcaact ggagcacgga
     2101 aaagaaaaat atggagatac agtactgagg cagaatatac gaaaagagga aaagcagatg
     2161 aagataggaa acaaagaatt tgatctgaaa aatcatgctt atgtgatggg cattttaaat
     2221 gtgacaccgg attctttttc cgacggcggg aaatggaacg ataagggtcg ggcaatgcgt
     2281 catacacagg aaatgataga acagggagca acgatcattg atatcggtgg cgagtccaca
     2341 agaccaggtt at
//
LOCUS       DAWDWH010000258         2309 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_14846_length_2309_cov_10.247560, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000258 DAWDWH010000000
VERSION     DAWDWH010000258.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14846_length_2309_cov_10.247560"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          616 a          547 c          488 g          658 t
ORIGIN      
        1 aatccgggtt cagaaaatct acagaatgga tcactgaacc agaacaaaat ctcatggatg
       61 aatgacagca ggctttccgg cattgatccg gcaaagctac agatgctctt ttctatgagc
      121 ggccaggggg catcgaaaag tcagaatgaa ctgatgccgt ttcttatggc tgtcgccgca
      181 cgttccaggc aaaacggtat gacctttaca ccggaagaaa ccaatctgat catagaagtt
      241 atgaaacagg gaaaatcaaa agaagaaata gccaggattg aaaaaatccg catgatgatg
      301 cgtctgatca aataacagaa gcataaaaaa gcttccagac ggatttctcc gaaatggaag
      361 cttttgtttt attttccagc ggtttctttt tccaggatac agctgcgaag taaggtcagg
      421 gcattgaccg tagaaagctc acggatcttc tgacggtttc ctttgaaatg aaattccctg
      481 atgcgaagtt cattctgata tgcacatgcg atatagacaa gcccgaccgg cgtcgtttct
      541 gtccctccgt ccggacccgc aagtccggtg atggaaacgc agatatcgga accagtgata
      601 aatgccccat ttttggccat ttctttggct gtcttttcac tgaccgcccc atatgttttc
      661 agcgtgttct tttttacatc gagcaatttc cgctttgcac ggttggaata ggcaacaaat
      721 ccctgcttga acacttctga aacaccggaa acattggtca gtcttgctga gagcatacca
      781 cctgtacagg attcggctgt cgtaagtgtc agatttttct cttttaaaag tgccacaatg
      841 ctctcttcca gtgtcgttgc ctcatccgtc gcataaatat gactgccgaa gcgttttttc
      901 aattctttga gcatcggttt taccagcttt ctggcttctt tttcatccga ggctctggca
      961 gtcacacgca gatggacttc gcctgttttg gcgtaagtgg caatcgttgg attttcctgt
     1021 gcctcgatca gatcgagaat ctctgtctct accaggctct ctcccacacc gcacaatttc
     1081 accatgacgg aataaatagt ttccggctgc agtctgttca gataaggaaa aatattctga
     1141 tgaaacatcg ggatcagctc atttggcgga cctgggagca ggatcatgga ctttccatct
     1201 ttttccatga taatgccggg tgcggttccg ttgtggttgt cgatgacgat gcatccttcc
     1261 ggaaccagag cctgtttcca gttgttgtct gtgataattt taaacggact gtttttaaaa
     1321 tattcttcga tgcgttcttt gctgtgtgca tcttctacca gtggaaaccc catcacagca
     1381 gcagccgtct ctttggtcag atcgtcctgt gtcggaccaa gtccgccgca gaggatcacg
     1441 atatcagaac ggtcaagtgc cgtccggatc gtttctttta atcgctcttc attatctccg
     1501 accacagact gatgatacat ggataatcca agcatggcac actgtctggc cagaaaagca
     1561 gcgtttgtat tgacaatgtt tccaagcagc agttctgttc caacacatac cagttctaca
     1621 accatgttgt aaacctccta acgtattttt aattctgcat tttcagaacc tgtttgtttt
     1681 tggcgatata atccacaagt gatactacgg taaggaccag tgcaagatag atcatcacct
     1741 gaccgatcat atcaaaaaca ccgccaaagt ctgcaaccag aagaatgatc atgatcattt
     1801 gagagattgt ttttgctttg ccccaccagc ttgcggcaat tacaatgcca ttatctgctg
     1861 cgatcagacg aaggcgatcc agatccataa ggcagatcat tgcagcacat accagcagtt
     1921 tatctgcgag cggatccata aattttccga aatttgtgac ctgattgttc tttcttgcaa
     1981 gataaccgtc cagagtatcg gtcaggctcg ctgcaataaa aataccgagt gcaatatact
     2041 tatccacact tgtgatatcg ccgagcataa acagtacaaa aaacggaatc ataatgacgc
     2101 gaagaaccgt taacgtattt ggcacattca ttttgttttc tcctgtcttt gaaatatagt
     2161 gaatgatgcg gctgacatca ttctgtgagc cataggtggc acttttgcaa atcccataaa
     2221 cgcaggtgcg tatacagaca tctgttttgc atctgtttct cccttgaccc attccaaaca
     2281 attatacggc aagaaaacct atttgtcca
//
LOCUS       DAWDWH010000259         2285 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_14991_length_2285_cov_11.862780, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000259 DAWDWH010000000
VERSION     DAWDWH010000259.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14991_length_2285_cov_11.862780"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          492 a          588 c          476 g          729 t
ORIGIN      
        1 gcacagacaa accacatacc ttccgccttc agttcttcga tcgtcttgcc gatgttggtt
       61 acttttgcaa ccggtgtgta gttcaatgct cctgcggaga ctctggctac ggttgctgtc
      121 agtccgactg ctcttctttt cgggatcaca acgccgtgtg cccctgcaag gtttgcggta
      181 cggatgatcg cacctaagtt gtgcggatct tcgatgttat ccaggaaaat gataaacggt
      241 gcttcgcctt tgtcttttgc gttctgcaga atatcagaaa tttctgcata ctcataggcc
      301 gccgcacttg cgatcacgcc ctgatgatgt cccgtctgag aaagctgatc cagacgttct
      361 tttttcacaa actgaacaat cgtatcatgt tttttggctt cccgcaggat tgttctcacc
      421 ggaccgtcct gacagccgtc cagtacaaac agtttgtcga tcgttttgcc ggagcggaat
      481 gcttccagca cggcatttct gccttctatg gtaagttctt cgtatctcat gcctgttcct
      541 gacctttctt tttctgactt tctgccatat caagaccggt ctttatcagc tcgatgatac
      601 gtttcgtatc ctctttcaga taaagatatc ccatcaaggc ttccagtccg gtcgctttac
      661 ggtaatctgc caccgttgca ttttttgctt ttgtgtaaga ctttgcattg cgtccgcgtt
      721 tgtatatcgt gatttcctct tcgctcaata ccggcatcag gttttcgatc atctctgcct
      781 gagcagaagc tttgacgagc tttgatgcac gctcatgcag cttctgcggc ggacagttcc
      841 catgtttgac cagaagtgtg cggatcacca ggtcaaaaat agaatctcct atgtatgcca
      901 gtgtcagcgg ggaataagtt ctcagatcct gatcttccag ctgaaattct tctttcaggt
      961 attctacaaa agaatctatg ctcttttcca ttttactcct tctcttgtgt cctcaagaac
     1021 gattcctttt tccagcagtt ctgcacggat ctcgtctgct ctctggaagt ttctctcttt
     1081 tcttgccacc tgacgctctt cgatcagttt ttcaatctct tcatccagga tctcttcttt
     1141 ggtctcgaaa acaagtccca ggatatcgcc aagctccaga agttcatcca gaagactctg
     1201 tacaaatgct tttgtgctgt tttcattcac attgctgttt gcaagacgca ccagctcaaa
     1261 tacaactgaa atggcatctg cggtattgaa gtcgtcatcc atggcatcat caaatttctt
     1321 tgccaggttc tttgcttctt ccagtgctgt catctcttct gccgtcatct ctgcctcttt
     1381 tgcagctccg gcataatgtt tcaggctgtc tgccgcatta cagatacgtg caagtcctgt
     1441 ttttgcagac tccatcagat cagcactgaa attcagcgga cttctgtaat gtgcactgag
     1501 catgaagaaa cgcagtacct ggaggtcata tttctcactg atctcacgca cggtaaagaa
     1561 attgccaagg gatttggaca ttttgcggtt atcaatattt aagaacgcat tgtgcagcca
     1621 gtatctggaa aagatcttgc cgttgcaaca ttcgctctgt gcaatctcat tctcgtgatg
     1681 tgggaatacc agatcctctc cacctgcatg aatatcaatc tcttcgccca gatatttttt
     1741 cgccataacg gagcactcga tatgccatcc aggacgtccg tcgctccacg gagacggcca
     1801 ggacggctcg ccttcttttt tcggtttcca cagtacaaaa tccagcggat cttctttctg
     1861 atcttctccg gttaccagaa gggaacggtt tccggaacgc agatcatcga gatttttatg
     1921 ggaaagtttg ccatattctt caaaacggcg tgtgcggaaa tatacggtac cgtttacgtt
     1981 gtaagcatac cctttctcga taagcgtgga gatcatgtcg atcataccgt caatttcctg
     2041 tgttgccagc ggatgtgtgg tggccggttt gacattcatg cctgccatat cctttttgca
     2101 ctctgcaatg taacgctcgg aaatctcttt tgctgtcacg ccttcttcgt ttgcctttgc
     2161 aatgatcttg tcgtcaacat cggtaaagtt ggatacataa tttacttcat aacctttatg
     2221 ctccaggtaa cgtctgacgg tatcaaacac gatcatcggt cttgcatttc cgatgtggat
     2281 cagat
//
LOCUS       DAWDWH010000260         2268 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_15113_length_2268_cov_10.482151, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000260 DAWDWH010000000
VERSION     DAWDWH010000260.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15113_length_2268_cov_10.482151"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          518 a          593 c          453 g          704 t
ORIGIN      
        1 aatcaggaaa ttggcgaaat ctccgacacc tgcggtcgat gatcctttaa aaagcatgtg
       61 cagtacgact ttgatcagtt cgataaacat gcctgcgacc ggtcccatcg caaaggtacc
      121 gatcagaacc ggaatttcac tcaagtcaat cttatagaaa gacggtgcaa agaaaagcgg
      181 tatttcaaac atcatcaaca caacggaaac cgcccccaac ataccaatct gcaccagatt
      241 tcttaagtta cttctttctg cagttgtttt cactgcattt gtacctgaac tgttcatacg
      301 tccatctctc ctttttgaat ttaattcaca ggaagatctt cgtctgatac gatgccatca
      361 ttttgaaata aaaagccctg aatctgttct acagattcag ggctttgata taccatcgta
      421 tgttcttctc tcatccagac tttgactgtc ggtcatggaa tttcaccatg tcagccgctt
      481 acgcgggtcg cggactatac cgccggtggg gaattacgcc ccgccccgaa gatttctgct
      541 atctgcaatt ataagataat tttttctttt gtcaagcatt ttcggaaaac ttttacaatt
      601 tcatggacag agaaattctt tttttcttga ggtcgatgcc gatcactttc acatcgatga
      661 tatcgcctac ttttacgaca tccagcggat gttttacgaa ctttttactg ctcatctcag
      721 agacatgcac cagaccgtcc tggtgtacac cgatatccac gaatgctccg aaatcaatga
      781 cgtttcgaac ggttccttta agtaccattc cttcttttaa gtcttccatc gccagaacat
      841 cgcttcgaag gatcggtttt ggcatttcat cacgcggatc tcggccaggt ttttccagtt
      901 ctgccgtgat gtcacgcagt gtcggctcgc cgatttccag ctcttctgcc atttttttat
      961 aatctctgat ctttctggaa attccgttta aatttccggc tgtgatgtct tcttttgtat
     1021 agccgagaca ctccagaagt ttgcctgctg ccgcatagct ctccggatgg actcctgtgc
     1081 agtccagtgg attttctccg ctgcgtatcc tgaggaatcc ggcacactgc tcaaatgctt
     1141 ttggtcctaa ttttgcaact ttcagaagtt cttttctgtt ctggaagctt ccgttttctt
     1201 cgcggtatgc aacgatattt ttcgcgatca ctttggaaat accggaaaca taggaaagca
     1261 gggatgcgga tgcggtattt aaatccacac cgacacggtt tacacagtct tccacgactc
     1321 cgcccagtgc ttcgcccagt tttttctggt tcatatcatg ctgatactgt ccgactccga
     1381 tggattttgg ctcaattttc accaattctg ccagcgggtc ctggattctt ctggcgatag
     1441 atgcagcact tctctgtcct acatcaaaat tcggaaattc ttctgttgcc agtttactcg
     1501 cagaataaac agatgctccg gcttcgttta cgatcacata ctgcactttt tccggaatct
     1561 catggaaaag ctctacgatc acctgctcag attctctgga tgccgttcca tttccaacgg
     1621 aaaagagcgt gatgtggtac ttgcggatcc attcttttaa tttttctttt gccgcctgga
     1681 tctttttttc attagtcggt gctgtcgggt aaatgacggt cgtatccagc acttttccgg
     1741 tcgcatcgac aaccgccagc ttacatccgg tacggaacgc cggatcccag ccaagcacaa
     1801 cctgtccggc aatcggaggc tgcatcagaa gctgttccag attcttgcca aacacctgta
     1861 tcgcaccatc ttctgcctgc tcggtcagtg cattgcgaat ctcacgctct actgccgggg
     1921 cgatcagtct gcggtaactg tcctcgatca ctttttccag gaattcggaa gtctgcggat
     1981 tttcacgcac catgatctgt tttcttaaat aaccaagaat ccgatcttcc ggagcttcca
     2041 gttttacggt caggaatttt tccttttcgc cgcggttgat cgccagcaca cggtaacctg
     2101 ccatttttgc aactggctct tcatactgat agtacatctc gtaaacagaa gctgctttct
     2161 catcttttgc ttctgtcacg attttaccct ctttctgtgt caggctacgg atgcggatac
     2221 ggtaatttgc atcatctgaa atcgtctcag ccagaatatc acctgctc
//
LOCUS       DAWDWH010000261         2264 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_15142_length_2264_cov_8.403803, whole genome shotgun sequence.
ACCESSION   DAWDWH010000261 DAWDWH010000000
VERSION     DAWDWH010000261.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15142_length_2264_cov_8.403803"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    233..242
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1084..1093
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          744 a          426 c          585 g          489 t
ORIGIN      
        1 cgacaacctc agtagccgga aatgcggaat ttacagcgga agggataaat aaaggcagag
       61 gacttgccat tttaaaagaa cgtcttgcga ttccaaaaga gcagatcatt gcgatcgggg
      121 ataacgaaaa cgatgtggag atgttcaaag aagcagggat ttccgttgcg atggaaaatg
      181 caaaagatcc ggtaaaacag caggcagatt ttgtggctgc ggataatgac cannnnnnnn
      241 nncgacgggc atttaccggg cacttgcgaa actgaaaatc gtccggtaaa tgcagaagcg
      301 aattgttgat gcaaaatagg ataaagcact tcacaattac ttgacaatat gcacaaagca
      361 atgtaaaatt aatatgttgt acttgtacaa tgcgagtaac aaaaaggcat ttaaagaaac
      421 attgcagctg tgaagataaa gcagttacag atgctttaaa tccgtccgtt acttgaaaat
      481 tctataagga ggtgctcctg tgggcccagt agtaattgct attgctgttc tggtgactct
      541 catcatcgcc gtccctgtta cagctgtcgt agcgatctct taccgcaaga aagtggtaga
      601 gacaaagatc ggcaatgccg acgagaaggc aagacagatt gtagatgacg ccttaaaggt
      661 tgcagagaca aagaaacggg aagcactgct cgaagtgaaa gaggaatcct tgaaaaccaa
      721 gaatgaactg gaaagggaaa caaaggaacg tagagcagag ctgcagcgtt acgaaaaacg
      781 tgtactgtcc aaagaagaag cagtggacaa aaaagcggac gcaatcgaaa gaagagagac
      841 aggcttagcg gcaaaggaag aggaaatcaa aaagagaagc aatgaagtgg aaaagctgac
      901 agaacagaga gtacaggaac ttgaaagaat ttcaggattg acctccgagc aggcgaaaga
      961 atatctttta aaaacggttg aagatgaagt aaaacatgac actgccaagc tttataaaga
     1021 gcttgagagc agagcaaaag aggaagccgg caagaaagcc aaagaatatg tggtcaatgc
     1081 catnnnnnnn nnnctacgat ctctgttgta cagcttccga atgacgaaat gaaaggaagg
     1141 atcatcggac gagaaggcag aaacatccgt acactggaaa ccatgacggg tgtggatctg
     1201 atcatcgatg atactccgga agcagtgatc ttatccggct ttgatccgat ccgccgtgag
     1261 gtggctcgta tcgccctcga aaaactgatc gtagatggca ggattcatcc ggcaaggatc
     1321 gaggagatgg ttgagaaagc acagaaagaa gtagagaaca tgatccgtga ggaaggtgaa
     1381 gcagcaacac ttgaagttgg tgtgcacggc atccatccgg agctggtgcg tctccttgga
     1441 cgtatgaagt tccgtaccag ttatggacag aatgcactga aacattccat cgaggtggca
     1501 cagctttccg gacttctggc aggtgagatc ggtgtggatg tccgcatggc aaaacgtgcc
     1561 ggacttctgc atgatatcgg aaaatccatc gaccatgagg ttgaaggttc ccatatccag
     1621 attggtgcag atctttgtaa gaaatacaaa gaatctccgc ttgtcatcaa tgcggtggaa
     1681 gcacatcacg gagatgtaga acctgagagt ctgatcgcat gcatcgttca ggcagctgat
     1741 acgatttcag cagcaagacc gggtgcgaga agagagacac ttgagactta cacaaatcgt
     1801 ttgaaacagt tagaagatat taccaactcc ttcaagggag tagacaaatc gtttgctatc
     1861 caggcaggac gagagattcg tatcatggta gttccggaac aggtcagcga tgctgatatg
     1921 gttcttctgg caagagatgt tgcaaaacag atcgaggcag agctggaata tcctggacag
     1981 attaaagtca gcgtgatccg tgaaagccga gcggttgatt acgcaaaata gcatttaaac
     2041 ataccccgca taccatagcg atatggtttg cgggattttt ttgtgctgat tgcatcagat
     2101 tctgcatgtg tttgacttga aataaggcta ccgcaatccc actggcttta gacggtgggt
     2161 taaggtagcc aaaaatcaga aattatagta tactcatggc atgggaacct ggaaatctaa
     2221 aaacagacac aaatacctat tacaatatca cattattttt gtct
//
LOCUS       DAWDWH010000262         2258 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_15173_length_2258_cov_10.790740, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000262 DAWDWH010000000
VERSION     DAWDWH010000262.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15173_length_2258_cov_10.790740"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1698..1707
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          616 a          474 c          580 g          578 t
ORIGIN      
        1 ttttgaacaa caggaaaaat ggcatggcaa tgatgttgct gtttatcatt ttttatgcag
       61 cagctgcagg catcatgctt gtcagtgcga ttaccgagtt gattccgctt atgctactgg
      121 cgatcatctg gatttccatc ggctggatcc cgtttctggg acttaaagtt ctgaaaccgc
      181 aggaagctct ggtgcttact ctgttcggta agtatgtcgg cactttgaaa gatgcaggat
      241 tttactgggt aaatccattc tgcagcagtg tgaacccggc agcaaagacc aaactgaacc
      301 agagcgggga tgtggatggc ggaaacagtg cgaaatccgt gctggttgcg gcaacaggtg
      361 caaatgcttc tgcaaatgca tcggttccaa acaaaaagat ttccctgaag atcatgacac
      421 tcaacaataa ccgtcagaag atcaatgatt gcctcggtaa tccggtggaa attgggatcg
      481 ctgtgatgtg gcgtgtgacc gatacggcaa aagctgtttt ccatgttgac aactacaagg
      541 aatatctttc cctgcagtgt gacagtgccc tgcgaaatat cgtgcgtatt tatccttacg
      601 atgtggcacc gaatgtcgat acgaccggtg acggcattgc cgatgaaggc agcctgcgtg
      661 gttccagcga aattgttgca ggacgcatcc gcgatgagat ccagaagaaa gtagcagaag
      721 ccggtctgga gatcatagag gcacgtatca cctatcttgc ctatgcaccg gaaatcgcag
      781 ccgttatgct tcagagacag caggcatcag cgatcattga tgcgagaaag atgatcgttg
      841 acggagcagt cggtatggtt gagatggcac tggagcgtct gaatgagaat caggtagtcg
      901 aattagatga ggagcgaaaa gctgccatgg tctccaatct tcttgtcgtg ctctgtggaa
      961 atcacgatgc acagccggtt gtaaattcag gaagtctgta ctgatatggc agagggcaga
     1021 ccgaagaaac agatcccgct caggctctcg gcaaaactgt atgatcagat cgcagcctgg
     1081 gcagaagatg attttcgctc cgtaaacggg cagattgaat atctgctcac agaatgtgta
     1141 aggcaacgta aaaaaaacgg caagtatgta tccgaaacaa tggatgaacc gctggaatta
     1201 gacatctgag atgtaaaaag aaaaagtgtc ggaagaatga aaggttcttc cggcactttt
     1261 gtgtcacttt gtatttaaaa atatttttgt aatcttaacg tttttcaatc ggaacataat
     1321 ctttaatctc agaaatgcct ttgtatgcag gacgaataat ttttccgcta ttggcaagct
     1381 cttcgaggcg gtgggcactc cagccaacca cacgggcgat cgcaaagatc ggtgtgaaga
     1441 gctgcgtagg cagatccagc atactgtata caaatccgct gtagaagtcc acgttggcac
     1501 tgacaccttt gtagatcttg cgttcttcgg caatgatctg tggtgcaagg cgttccacca
     1561 tagaataaag ctggaactct tcttctctgc ctttttcttt ggaaagtttc tctacgaaac
     1621 ccttgaaaat attggcacgc ggatcagaaa gagagtaaac agcatgtccc ataccataga
     1681 tcagaccggc gtgatcgnnn nnnnnnnaaa gcgtctttgt tcagcagggc acgcagataa
     1741 tcaccaactt ctttttcgtt gttccagtct ttgacggttt tcttcatatc ttcaaacata
     1801 cggacaactt tgatgttggc accgccgtgt ttcggacctt ttaaagagcc aagggaagat
     1861 gctatagtag agtaggtgtc tgtaccagaa gaagatacca catgagtggt gaacgtagag
     1921 ttgttaccac cgccgtgttc catatgcagc accagtgcaa tgtcaaggat gcgggcctcg
     1981 aggttggtgt atttactgtc tggacggaga atatggagca gattttctgc agtggaaagt
     2041 gatggatcag gcatatggat ataaagactc tttccatcgt gataatggct gtatgcctga
     2101 tagccatata cggaaagcat cggaaacagt gcaatcagct gcaagcactg acgcagtaca
     2161 ttcggaagtg agatgtcgtc tgcacggtca tcgtaagtat agagtgtcag aacactgcgg
     2221 gcaagcgtat tcatcatgtc ctggctcggt gctttcaa
//
LOCUS       DAWDWH010000263         2201 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_15575_length_2201_cov_8.352283, whole genome shotgun sequence.
ACCESSION   DAWDWH010000263 DAWDWH010000000
VERSION     DAWDWH010000263.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15575_length_2201_cov_8.352283"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    570..579
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          636 a          421 c          618 g          516 t
ORIGIN      
        1 atgcattacc ggaggctgaa agaactggat ccggatgata tctgggaatt tttgcgagaa
       61 ggaggggatt tttgatggga aatctgtgga cagaagaaca gaagcaggtc atctggcttc
      121 gaaaccggaa tattctggtc tcggcggcgg caggttccgg aaagacagcc gttctggtag
      181 agcgtatttt gcagaagatc acggacaggg aacatccggt ggatattgat cgtctgctga
      241 tcgtgacgtt tacaagagct gcggcggcag agatgcgtga gcggatcgga ctggaaatcc
      301 agaaaaggct gctcgcagat ccggataacg agaatctgca aagacaggaa acgctgcttt
      361 tccatgcaca gatcacgacg atcgacagtt tctgtcagtc ggtgcttcgg acatattttc
      421 atctgatcga tctggatccg gcgttttcca tcgtggatga aggagaggca aaacttctgc
      481 ggcgagatac ggcggaagag cttctggaag aaaagtatca ggagggcgat acggactttt
      541 tacagtttgt ggaatgtttt gcaaacggan nnnnnnnnnc aaacggaaaa aatgatgaga
      601 atctgatcga aatgatctgg caggtctatg aattttccag aagtcatcca tggccggaag
      661 aatggctgga agaatgtaag aaaaattatg atattgatac actggaagat ctgatctcaa
      721 gtgaatggat gaaaatgatc tgtgagcagg tcgatcagac cctgaatgat ctggaaatga
      781 tccgaacaga agccctgaaa gtcgcaaatt cgccgtacgg accgtggatg tacgcagatg
      841 ccctggagca ggacggcgag atcttaaaac agctttcgaa aggcaatgat tacgcagaat
      901 atgccagacg ttttctgaac atcagaaaat ttgcagtgct ttccagaaaa aaagatgaag
      961 aagtatcgga tgaaaagcgg gaacaggtaa agctttgcgt gatcagatca aaaaaggcat
     1021 tgcctctttg caggagcagt atttttacca gagtccgcag gagatgttag aagagctgaa
     1081 ggcgggaaaa gtgtccgcac agatgcttct gatgctggca tccgagtttg gattgcggtt
     1141 tacggagaaa aaacgggagc gaaatctttt ggatttctca gatctggaac atctggcact
     1201 gcagatcctg gtgaaaaaag agaatggaaa tgtggtgcca ggtgaagcgg cactggcttt
     1261 ttcaaagcag ttcgaagaga ttatgatcga tgaatatcag gacagcaaca tggttcagga
     1321 gctgatcctg accagcattt ccagatgcag catggggcag aacaatctgt ttatggtcgg
     1381 tgatgtcaag cagagcattt accggttccg tcttgcctgc ccggagctgt tcatggaaaa
     1441 atacgatacc tatacgacgg aagactccga aaatcagaag atcgagctgc acaaaaattt
     1501 ccgaagccga aaggaggtgc tctttgatgt caattttatt tttcgcaaga tcatgcaccg
     1561 gaatctcgga aatgtagaat atgatgataa tgcggcactc tatgccggga tcgaatttcc
     1621 gaaactggtc accaatgttg agacggatga tctgacggca gcaaaatctc cgctttccgg
     1681 aagtgagatc ctgctctttg atgcagattc ggtgacagag acaaatgcaa gagaggccga
     1741 agcacgtatg atcggcggac agatcaaagc gatcgtcgga agagagctgg tctatgacaa
     1801 agaaagcagg cattaccgaa aagcagaata tcgcgatatc gtcattttgc tgcgttcctt
     1861 aagcggctgg tcggaagaat ttctggcagt tttatcggat atgggaattc cggcgtacac
     1921 cgggacgaga agcggttatt tttcggcact ggaagtgcgt acggtgctgt cctttctgcg
     1981 tgtcttggat aatccaagac aggacattcc gtttaccgca gtgctgacgt ctccaatcgg
     2041 cacgtttgcg acagaagaac tggcgaagat ccgtacggag aatccggaag tttcttatta
     2101 tgaagcgtgc cgggcgtatg aaaatgcggg cagcgacggg attttaagag aaaaacttca
     2161 gaagtttttt aaagtgctgg aacatttccg cagttgtgtg c
//
LOCUS       DAWDWH010000264         2166 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_15858_length_2166_cov_6.945997, whole genome shotgun sequence.
ACCESSION   DAWDWH010000264 DAWDWH010000000
VERSION     DAWDWH010000264.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15858_length_2166_cov_6.945997"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    558..567
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1321..1333
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          478 a          522 c          435 g          708 t
ORIGIN      
        1 gctcgatatc tgcttcacac tcatcacgca gacacgcaag gaacggtgtc atctgctcgc
       61 tcagacgttt attggtgcca tctgtcagat attttacagc tgcatcgtaa atggtcttga
      121 actcgttacg catatcatcc aactctgctg tcgtaactgt tcctgcttct acttttttct
      181 ggatctctgc cagcttgttt ttcaggtcaa cagactcttc aaattttgcc tgtcttccgg
      241 ctgtctgaac cggtccctga tagatcatgt gttttgccac ttcacgcagg gctgtggaag
      301 aatctgattc cagtgcggta acatggtcaa tgtacttaaa ggaatcttcc catgcctggt
      361 ctccttcttc tgtggcatcc catggtttcc agctatagtc tgctgcggta aagatcgcta
      421 ctttggaagg ttctgagtgc tgcatcgggt tgaggatgat accttcatat ttatgaccgt
      481 caattccggt atgcaggatg gtattgtgac cacccatgat cagacctgtt ttatagttat
      541 cgttgcacgg ccagtttnnn nnnnnnnctg caacgccatt ggatgctaca ttgttataga
      601 agttatctgc aaagtttgca cttacctgtc cccatactgc attaccggtc atcagcatat
      661 gtacctgtct gttggcagaa gcattgatgc ctttcagttt tgcagaggaa ccatttccca
      721 tgtagtctgt cgggcagtac aggatatcgg ttttcagatc cggatatttg gtgtttttca
      781 gttctgtcag ccagtttgtc aagtcattga gcagtctgct taagtttgct gcacttgatc
      841 cggtcgcatc atcctccaga agggcaatct gacgcacgcc ggattcgatc acctgcagat
      901 attttgcttt taccgttgcc agatctgcat cgtaattgtt gaaattaaat ggactgttca
      961 taaacgtatg cagagcataa acgaagaaac atttgctttc attgcctgcc tgtgcaagtt
     1021 ttttgatctt ctcaagatct tcctttggat acagctctct ccactgtttg ttgtggtaag
     1081 gatcgtcttt cggtgcgaac acatactgat tcatcttgat ctcgccgccg aatttcatca
     1141 gatcaatacg gtcttccagt gaccacggat ttccataata tccttcgata aatccacgga
     1201 actgtacttc tgcatagtct gtcaccgtca gctctttgat ctttttgtct gcggaaagct
     1261 gttcaaaaat acgtttcagt gtggtgacac cgtaaaatgc cgcatcggtg tctttaccaa
     1321 nnnnnnnnnn nnncttgccg tctttgacat acagcatata agcatcgtaa ttatcaaagg
     1381 tcgcctgaac cgctccgttt gcttttccgt aagtatctgc tgtatccccg gaaccataaa
     1441 caccaacgat cagctcggtc ttgtctgacg ctgcactttc gtttccgatc agtttcagca
     1501 cattcagcgt gtctgtgata cgtgtttttg tgtaatcgtc aatgtcactt ccgatcgaca
     1561 gtttcacctg atcggtaagc tgcatttctt tgtcaccgta ggtgatctcc tgcggtgtcg
     1621 ggtagatctc atactcacct gctgtttctt ttacgcctgc ggatacagga atacagaaca
     1681 gcataagcat tgccagaaaa gcaacgagct tcatacccag tgatgttgtt ttctttttca
     1741 tgtgtttcct ccttttcttt ttgtacccct gcacaaaaac ctctttatct tagtcataaa
     1801 caggcaggga tacgttactt ttactataac aatttccgta agttgcgtca actgttatct
     1861 tcaatctctg tcggaaaaga tgcctgcttc tgccgggatg aatacatgat ccagaactgg
     1921 atctgtcggc tgctttgcat tttttaccgc ctcttcacag aaatagtcct gtgcacagat
     1981 cagcatcttt ttccctcttt tatcaatctt ttcatagctt ccgtccggct gcaggatatg
     2041 agccttcaga ttatcagcaa gctggatatc cagaatatgt tttgcctgct gtttgatctt
     2101 ttcatctttt aatggaaata cgatctcaac acgtttgtcc aaatttcgcg gcatccagtc
     2161 cgcact
//
LOCUS       DAWDWH010000265         1838 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_18658_length_1838_cov_9.515423, whole genome shotgun sequence.
ACCESSION   DAWDWH010000265 DAWDWH010000000
VERSION     DAWDWH010000265.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18658_length_1838_cov_9.515423"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1138..1147
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          376 a          545 c          322 g          585 t
ORIGIN      
        1 cttatatggt atcgtaagcg gaacattttc aaaaactctg gcattatttg tgataacttc
       61 cccgatctca ccgtttccgc gtgtgactgc cttaaaaagc gtaccttcac ggtatgtcag
      121 tacgacggtc agtccatcca ttttccagga gagcagcact ttatgggcac cgacaaaatc
      181 tttcagcact tcgacttctt ttgtcttgtc aagagaaagc atcggctgct cgtgtcgctc
      241 tttcggaagc tcttcgagca cttcataacc gacctgtctg gtcggacttc ctgccaggac
      301 gatcccggtc tcttcttcca gtttctggag ctcgtcatac agccggtcat actcaaaatt
      361 gctcatgatc tcacggcttt cctgataata ggctttccct gcttttgtca gaagctctgt
      421 cagctccttc atcctctcgc gttttttgtc catgttcccc tcctgctctt tcccgctttt
      481 ttacttcctg cccgtaactg ccggtaaagt tcctgcattt ctgcttccgt cacatcccgg
      541 tactgtcctt ttggcagatt accaagctca atgttcataa ccctcacccg caccagtttc
      601 ttcacacgac agccacaata ctcgcacata cggcggatct gtcggttcag tccctgcgtc
      661 agaatgatac ggaagcggta ttttccgatc ttttccacct tgcacggtct ggttctggca
      721 tccagaaccg ggatcccacc ccggctcatc tgctcgacaa attcttctgt gaccggttta
      781 tccaccgtca ccagatattc tttttcgtga taattaccgg cacgcatgat ctgattgatg
      841 atatctccgt tgtttgtcac caaaagcagt ccttcggaat ctttatccag ccttccaacc
      901 gaaaaaacgc gtgtcggata ctgaagcaca tcctccacgg tcacatcgtt ccagcgtctg
      961 tccgtcgtgc agactacgcc ctctggtttg tgaaacgcca gcagaaccat tttctgccct
     1021 ctgctgcatc gttttccgtt gacggtgatc tccatctcgt ccgttacctt cctgccattt
     1081 tctgcacgca cgccgtccac aaatactttt ccttcttcga tcaggcggtc tgcttcannn
     1141 nnnnnnntag tacagtataa cacgttgtac aactgccgtc aaacactctc ttttatccgt
     1201 tttttgcact ctttttttct tttttccgac aatagttctg caaaatccga atattctttt
     1261 cttcaagatt taaaaattcc cggacaatcg tttcagactc ttttccgcaa atttccagct
     1321 tctgcgttgc tttctgcata tcacttctgc gaagccgttc ttcttttgcc agcatattcg
     1381 ccatatatcc ggtatttaca tttaatgccg tcttcgcctg cagcattccc caccgcttcg
     1441 cccgctccat cacaccaagc agcggcggaa catttcccgt ctcaaacaga cagtttgccg
     1501 ccttttcttt gatcccggaa tagcctgccg cctgtatgtt cagatccagt gccagctcat
     1561 cgtcatagac ctttcccaac acagtataaa tatcctgaag cgacctgctc gtatttttca
     1621 caatggtttc cagtaactcc tgttccgcat aagaaatcat atcctgcctc cttgcatcat
     1681 ctctctgcct ttttgctgat ttaggatatg catttttctt tttttctata cttcagcttt
     1741 tcatactgca ctcattgaat ctgagcatgc tgtattctct cacagtgaac tacccattgt
     1801 ctaaagccaa tgggcttcct gcttcgcaga cctcgtaa
//
LOCUS       DAWDWH010000266         1815 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_18883_length_1815_cov_16.641477, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000266 DAWDWH010000000
VERSION     DAWDWH010000266.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18883_length_1815_cov_16.641477"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          541 a          328 c          509 g          437 t
ORIGIN      
        1 aatatgttca cagccggaag ggagaagagt ttgaggtgca ggcgtttacc ggagtggaaa
       61 gtctctgtgc gtgtgcgaaa gagaaaacaa tagagattct gctgatttcg gggaaattat
      121 tgtgtgaaca ggtcagggaa ctgcagatcc gcaaagttat ggttctttcc gaaggagagc
      181 agatctgcga actgtcggaa tattccgcag tttataaata tcagccggcg gacagtctga
      241 ttgcggaggt catggattct tatgcgagag taaaggaagc accggcagct gcacttttca
      301 agccggaagt ggaagtgacc ggtatttatt cgccggtcaa acgcaccggt aagacttctt
      361 ttgcactgac actcggacag cttctggcaa gtacaaaagc ggtcctttat ctgaatctgg
      421 aagaatatgc cggttttgat gtattaatga accgacagtt tgacggggat atcagtgatc
      481 tgatgtattt ctccggaagt ggcaaaggca atctgatcag taaacttgga ggacttgtgc
      541 agacgatcaa caatctggac tatattccgc ccgccttttc cccgtttgat ctgcggagta
      601 tcaagtgcag cgaatggctg ggactgatcg aagatctctg ctcctacagt tcgtatgaag
      661 ttatcctcct ggattttgga gaacaggtgg ataaccttcc ggaacttctg aatcgctgtg
      721 gaaaaatcta tatgccggtc agagaggaca gcatggctgt gacaaaaatt gctcagtatg
      781 aaaaggtcat ggttgcaaga gaatatgaag aagttcttgg gaaaaccata aaactgaagc
      841 tgccgtttca cagcagtttc ggtaaaaagg aacattatgt ggagcagctg atctggagtg
      901 aacttggaga ttatgtaaga cagttaatac gcaaagaaag cgggtgatgc attgtggagg
      961 gaatggaaca gaaacaggaa aaactgtatg cccttttaaa cgagaaactg gatcatacgg
     1021 aagaaatatc ggatgaggaa atccttgtgg aaattgataa gctgatctgg gaggaagcaa
     1081 aagggcagta tatgccgctg tcagagaaaa tcacgctcag gcagcaactg tttaacggaa
     1141 tccgccgact tgatattttg caggaacttc tggaagatga gagtgtgaca gagattatgg
     1201 tgaatggaac agacggcatt tttgtggaga gagaaggcag aatacgttgc tgggagcatc
     1261 gttttgtttc gaaaacaaaa ctggaagacg ttgtgcagca gattgcagga aaatgcaacc
     1321 gcattgtcaa tgaggcgatt ccgatcacgg atgccagact ggaaaacgga gatcgtgtaa
     1381 atattgtact gccgccggtg gctgtcaatg gaccgatcat tacgatacgg cgttttccga
     1441 aagagccgat cacgatggag cgtctgatcg agcttgggag tgtttcaaaa gaggtagcgg
     1501 actttttgaa acaacttgtg tgtgccggat acaacatttt tatttgcggc ggaaccggtt
     1561 ccgggaaaac aacgttcctc aatgcacttt cggattatat tccaaaagac gagcggatca
     1621 tcacgattga agataacgca gaattacaga tacagggcgt ggaaaatctg gttcgtctcg
     1681 aagcaagaaa agaaaatacc gaaggaaaac atgcagttac gatccgggat ctgatcaaga
     1741 gcagtctgcg aatgagacca agcaggatta ttgtcggaga ggtgcggggc gatgaagtcg
     1801 ttgatatgct tcagg
//
LOCUS       DAWDWH010000267         1700 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_20209_length_1700_cov_7.250456, whole genome shotgun sequence.
ACCESSION   DAWDWH010000267 DAWDWH010000000
VERSION     DAWDWH010000267.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20209_length_1700_cov_7.250456"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          474 a          361 c          471 g          394 t
ORIGIN      
        1 aaccactcct gtgcaagcac atcgtggttt caggcttttg accctggcat catatatatt
       61 ttgaccataa cgacatccta tgttatagtt gtagttatga gaaagatatt ttatgagata
      121 gggacaacaa aaaaacagaa agaagatacc acaaagcgag aaatggtcct gttttgtttg
      181 agtgacctgc acgggacctg ttacgaacca ggaaaccggc cgatcatcga gtgtgcctgg
      241 aatcagaaac cggatctgat actggcttcg ggcgatatgt taagcggaga cagggaggag
      301 ggcgatgcca tagcagtggc tctgctctcc cgtctttcgg atcttgcacc ggtctgtttt
      361 gtaaacggaa accatgagat gtgtgtggag agacatcagc cggagcgata tcagaaattt
      421 atggatgatc tgaaaaacgg cggcgtgcag gttctgcaca acgagacggc atggttctgg
      481 atcgacggtg agaaagttgc cgtgaccggg tatgagctgc cgtgggaata ttaccggcgg
      541 ttcagccgca gacatccgtc cgttgtcggc atggagcgaa gcatcggaaa acgaaaacag
      601 gatgcatacc agatccttct gacacacaat ccggtctttt ttgaagaata tgctgcctgg
      661 ggtgcggact ttacttttgc cggacatctg cacggcggtt ttatccgttt gccgtatttt
      721 ggcggagtca tcagcccgca gttccagctg ttcccgaagt acgaccacgg tctgtatcag
      781 atggatacaa gcagcatggt cgtcggtgcc ggcatgggta atcattccgg cctgtttcgg
      841 atcaacaatc cgaccgagat cgttaccgtg cggatcaaga ggtgaaatta tggggattgc
      901 agtaaaatta caggcttttg aaggaccact cgacctgctt ctgcacctga tcgaaaagaa
      961 taaagtcagc atttatgata taccgatcgc tgagatcacc gaccagtaca tggcgtatct
     1021 ccatgaaatg aaaaagcagg atcttggcgt gatgagtgag ttccttctga tggcggcaac
     1081 gcttctggat atcaaatcac ggatgctgct cccgaaagaa gtgaacgaag agggcgaaga
     1141 ggaagatccg cgtcaggaac tggtgcagca gctgctcgaa tataagatgt ataaatatat
     1201 ttcttatgaa cttaaggaac gcaggcagga tgccgctcag gttctctacc ataagcagga
     1261 tctgccggaa gaagtgaagg catataagca gccggtatcc acagaagagc tgcttgcggg
     1321 cgtgacactt tccaggatgc accggatttt tgtggatatc atacggcggc agaacaaccg
     1381 catcgatccg atccgcagta cgttcggcac actgcagaaa gaagaagtag acatggatca
     1441 gatgcgggaa aaagtggcag aatatattga gcagcacgaa gtctgcaatt ttcgggaact
     1501 gcttcttgga caggcaacga aaatgcaggt tatcatagca tttctcatca ttctggagat
     1561 gatgaaggaa ggcgttatta cgatcgttca ggaggaaagt ttcggtgaga tccacatcgc
     1621 aaaaaacaag acagatgaaa gcggcagttg aggcgatcct gtttgccatg ggcggatcgg
     1681 tggaagtcga aaagattgca
//
LOCUS       DAWDWH010000268         1663 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Lachnospiraceae bacterium isolate gSpreadC_ID_3121
            NODE_20665_length_1663_cov_14.584577, whole genome shotgun
            sequence.
ACCESSION   DAWDWH010000268 DAWDWH010000000
VERSION     DAWDWH010000268.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996577
            Sequence Read Archive: SRR8800861
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lachnospiraceae bacterium (human gut metagenome)
  ORGANISM  Lachnospiraceae bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Lachnospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20665_length_1663_cov_14.584577"
                     /isolate="gSpreadC_ID_3121"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1898203"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1372..1381
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          533 a          359 c          410 g          351 t
ORIGIN      
        1 gagtagatat cgtacgtaaa gtgtcgttgg agggggaggc ttccgacgaa cgaaggttat
       61 accgcgttac ggtctcgctt ccgctactgc acaaagaacc aggatatttt atcctttatt
      121 tttgtgcggc atttttttac ccaaaaacag ctaattaccg caaaaagcgg ttaataaaga
      181 aaggagtaat gaacatcatg ggattcaaat cagacattga aattgcacag gagacaacga
      241 tggctccaat cacagaaatt gcagacaaag caggtattga ccgtaaatat ctggagcagt
      301 acggcaatta taaagcaaaa attgactaca atctgttaag agaaaaagaa ggcgaagacg
      361 gcaagctgat cctggttacc gcaatcaacc cgactccggc aggagaagga aagacaacca
      421 catctgtagg tctggcagac ggaatgtcaa gacttggtaa aaaagttatg gtagctcttc
      481 gtgagccgtc acttggacct gtattcggtg taaaaggcgg agcagccggc ggcggatatg
      541 ctcaggtagt tccgatggaa gatatcaacc tccacttcac aggtgacttc catgcgatcg
      601 gtgcagcaaa caacctgctg gcagccatga tcgacaacca cattttccag ggcaacgcac
      661 tgaacattga cccgagaaag atcacatgga agagatgcgt ggatatgaac gaccgtcagc
      721 tgagaaacgt agtagacgga cttggcggaa agacaaacgg aatgccaaga gaagatggtt
      781 atgacatcac cgttgcttct gagatcatgg cagttctgtg tctggcacgt gatatcacag
      841 acctgaaaga aagactgtct aaaatcatca ttggctatac atacggaaag atcgcagagc
      901 agaaaccggt taccgcaggt gacctgaatg cacagggagc tatggcagct cttctgaaag
      961 atgcactgaa accaaacctg gtacagacac tggagaaaac accggcaatc gtacacggcg
     1021 gtccgttcgc aaacattgct catggatgta actccgtaac agcaaccaag atgtgtctga
     1081 aactggctga ttacaccatc acagaagccg gattcggtgc tgacttaggt gctgagaaat
     1141 tcctggatat caagtgccgt atggcaggtc tgaaaccaaa cgcagttgtt atcgtagcaa
     1201 ccgttcgtgc actgaaatac aacggcggcg ttgcaaaagc agacctgaac aacgaaaatc
     1261 tggaagctct ggaaaaaggt atgccaaacc tgctgaaaca tgtaagcaat atcacaaacg
     1321 tatacaaact gccttgcgtt gttgcaatca atgcattccc gaccgataca gnnnnnnnnn
     1381 ntacgaactg gaaggttcta tcgaagataa actgaatgag atcgttcaga agatctacgg
     1441 cggaagcaga gttgtcctga cagcaaatgc aaagaaacag gcaaaacagc tcgaagatat
     1501 gggatttggc aactgcccga tctgtatggc aaaaacacag tatagcttaa cggatgacca
     1561 gacaaaactg ggtgctccga aagattttga gattactgtt cgtaacctga agatctccgc
     1621 aggtgcagga ttcatcgtag cactgaccgg tgatattatg acc
//
