LOCUS       DAWDXZ010000001        12971 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_1981_length_12971_cov_10.105838, whole genome shotgun
            sequence.
ACCESSION   DAWDXZ010000001 DAWDXZ010000000
VERSION     DAWDXZ010000001.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 12971)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12971)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12971
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1981_length_12971_cov_10.105838"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7574..7583
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8945..8954
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3232 a         3441 c         3234 g         3044 t
ORIGIN      
        1 ttcaccatag gagttccagc cgaggatggt cgtgaaagca aagagggtaa gtccgatgca
       61 gaggatgatg ctgccgtacg tcgggaaggc catcgtaaag gcggcattgg taagcgctgc
      121 cccgttgacg tcgcccatcc aggcaccgct gacgactaag acgaggcccg tcaaggtgca
      181 gatgatgatc gtatcgatga aggtaccggt catggagatg aggccctgtt cagccggcca
      241 tttagtttta gcagcagcgg caacgatcgg tgcactgccg agacctgatt cgttggagaa
      301 gacgccgcgg gctaccccat ttcgcatggc aaccaatacc gtagctccga ggaaaccacc
      361 ggcggcagcc gtcggagtga aggcgctgac gaggatctgt tcaatagctg ccggaacctt
      421 gtcggcaaag atgaccaaga aagcaaccgt cgaaataatg tagataatgg ccatagccgg
      481 aacgacttta gacgctacgc gggcgatgga ctgcagaccg ccaatcgtaa tggctgcaac
      541 caatacggtc aggacgactg ccgtatattc gacaggaatg cttgtcgtca gccgcgtaat
      601 ttcaacgata gagttgacct gagcaaaggt cccgataccg aagaaggcga ccaagacgcc
      661 gaagaaggca aacaagaaag ccaagggttt atatttttta cccaacccat tttcgatgta
      721 gtacatcggg ccgccggcga tttcgccgtt cttgtcgacc gtccgatact taacggctaa
      781 acaaccttcg gcgtatttcg tagccatacc gacgaaggca gccatccaca tccagaaaat
      841 ggctccggga ccaccggcat gaacagccgt tgcgacgccg acgatattgc ccgtaccgac
      901 ggttgctgcc aaagcggtgc acaaagcttt gaagctgtct acgtccccat cgccctgatt
      961 cttcgctgta aagatgagct tcaaggctct cggcaatttt gaaatctgca gcagacgaag
     1021 gcgagccgtc aataagatac cggtcccgat aagcaggatc aataaaggcg gtccccaaat
     1081 aaaactgtca atggcattca atgtttccaa catcttgcat actccccttt tagtgtattt
     1141 tagtataata acataaaaaa agacctatca tcatcactct gaagagatag gtcctaaaag
     1201 gtcgcagaaa aataaaggct ttcttccgct agctctgtcc ttttgcctga gagattagaa
     1261 acaatatcct tgcaccttcg gcgcccaaat gggactctcc agagtcgtgt ccacacacgg
     1321 tcccaccccc ttaggggcac ctgagagttt gactccgtcg gtaggctgtg ccttctcccg
     1381 tgtgtttcgt tcacgcaata tgtatgatgt gctgttatta taacgcggct gttcaaaaaa
     1441 tgcaagccct ttgtggaaat ttgtccgtat tgacgatact atcgggacaa ggactcccca
     1501 gagaaagcat aaggaaggag ttcttcccaa gaaacgaccg ctgtgtcgcc cttcatatta
     1561 gccatgataa tccgcggaat gcggaactcg gccatgactt gacggcaggc gccgcaggga
     1621 gcgcaagggc cgtccgtatc ggctacgaca gccagtgctt taaacgccct ctccccttcg
     1681 gaaagagcct taaaaatggc cgtccgttcg gcacagttac tcaggggata gctggcattt
     1741 tcgatgttgc agccgccaaa aacccggcct gactccatca ggacggcagc accaacggga
     1801 aagtgggaat aggggctgta cgaatgacgg cgatagtccc gggccagttc gataaggtcg
     1861 gcatcactca taaatagagg gctcccttca ggtggtccaa agcccgttct gccgtctgct
     1921 gccacttgag cggcgtcaag gcaatgtgcc cgtctcgaac gcaggccaca tcactgtcgg
     1981 ggggagccgt cgtcatgtcg atgtcgccga cgatacgata gcagacactg ccgtcgagat
     2041 cgcgtttttc agcgatgacg ttatggtaaa tctggacggt ctgggacgta atgcagacat
     2101 tttttaagct gatatcgcct ttcggaggta cgttaacgtt gacaatcccc ggaaaacgac
     2161 cttggagtgt aattttctga atcaactgcc acatcaaacg agccgtttcc acacagcgtt
     2221 cttcagtcat cccgatcatg gatacggcaa aggccggaat cccataatac ggcccttcca
     2281 tggccgctga caccgtcccc gaataaagga catcgctgcc cagattataa ccgtcgttga
     2341 tgccggaaat gatgaggtcc ggccggtcat ctttgagaaa atattccata gccatcttga
     2401 cacagtccac gggcgtaccg gaaacggcaa tttcccggat atcatcttct tcacggggga
     2461 gttcctgaca gtaaagggcc ttattaatcg tcatcgaatg ggacgcagca ctgcgctggc
     2521 cattcggcgc aacgacagtc agccggcagt catgggccga aagttctcgt tttaacgtct
     2581 gcaggcccaa cgccttcaaa ccgtcatcat tggtcaacaa aatgtgcata ataaagcctc
     2641 ctacttttta ttttccttcc gcagcaaggc tgcgctttca tacgacagtg ctcgtgtatg
     2701 cagggtgtga ctccatttca tcttatgccg atccttgaac ccgaggaagt tgacgggaat
     2761 ccagctgtac ataaaaatcg gatagatgat gagatacagc caagatttac gaggaacctt
     2821 gatttgcagt aagacgataa taggaataag atactgcccg acgccaataa tcgaccagat
     2881 ttgaacaggt acaatcgtat ttaaaatatt ggtataaaag ggccagtaag aattaagcca
     2941 ggccaacagg gcgtacaccg tcgagcccat cataaagaaa ggctgcgaca aggtaaggat
     3001 gccgtcaagc atgcgaatat tcccggtctt gatgccccga gcaaacaact taggaataaa
     3061 gcgctcgccg cagtcacact gcccttgagc ccagcgcttt cgctgacggc aggactgcat
     3121 gaagccaacg accttttcgt cataaatgat cgcgtcatga gcccaacagg tgcgcacgcc
     3181 ctcgaggagg accttcatgg agaattccat gtcttccgtc aagcattcgc agccccagcc
     3241 atacttgcgg acgatttccg tggcaatgca catacccgta ccgcctaaag ccgtcgacaa
     3301 tccgatgttg tacttggcca ggtgccacat gtggttaatc agccaaaaag cgatggaaaa
     3361 agttccggca atccaggaat cggtcggatt cttagaatcc atgtagcctt ggatgacctt
     3421 ctcccctttc aagaggtgat ggttcatttc ccgcaaaaat tcaggatgga ctaagttgtc
     3481 ggcatcaaag acgacgaagg catcgtactg tttttcttgg gccagcattt gagaaaacat
     3541 ccagtccaag gcgtatccct tcccgacttc ttccttattg gtccgaactt tgacgatagc
     3601 gccgtgatcc cggcaaatat cggccgtatt atccgtacag ttgtcggcaa cgacgtagat
     3661 gtcgtacata tccttcggat aattaagctg ttttaaattg tcgatgagct gccaaatgac
     3721 gcgctcttcg ttatgcgcac aaatgacggc agcgaacgta tgcttggggg ctgccgtact
     3781 gtgctcgcgc cgcttcatca agccgaagca ggccaacgta aagtaatata gtgaataaaa
     3841 aacgatgatg acttgtatgg gtatcataac gatatccaaa atatgttcca tatgcgactc
     3901 tccctaaata cttagcgagc tgctgcgacg ttcatggccg tattgactaa cccgaagata
     3961 atgtacagta agaatggcag ggtaataacc aagtgccaat cgacgtacag aaccaaggca
     4021 ccgataatca cagcaatagc cagcgccgac ttatggagca catcgggact ggcaccttta
     4081 aagtcggggt gatgaacctg gctgaccaga ttatacccca agaggacgac taaaatcgcg
     4141 accagccaag ccggaagcac ggccccggaa atgacgaagg tagcaatgag gcagccgccg
     4201 ttcggaatag gcatgccttg aaaatagccg tggacgacgc cggtattgag attaaaacgc
     4261 gccaggcgaa tgccgcccaa agcagcgtaa ataatagcag gaatcgcgcc gacccagcca
     4321 agggactgta aagaataggt ataaatcaag aacgccgagg cagccccaaa agatacgacg
     4381 tcagacaggg aatccagttc cttgccgaat tcaccggcca cgccgagagc tcgggccgaa
     4441 cggccgtcac aggcatcaca gacgacagcc aagaggatgc agatcccggc ccaaacgtat
     4501 tccccatgga gtgtcatcat catactcagg acgccaaaac tcagattacc tgaggtgaac
     4561 agacacggta ttatcttctt catgaaacaa tcctccctat caccgattca ccgccgcgaa
     4621 ctttatcgcc ttttttgacg gtgatttcaa cgttgtcacg tacatagatt tctgcacagg
     4681 acccgaattt tatcataccg tacagctggc cgtgctgtaa ttcatcgcct aacgtgaccc
     4741 aggagacaat gcgccgggcc aggacaccgg caataatatt gactaaaatg tccgtacgag
     4801 ttgaatgaat accgatagaa tagcgttcat tttcataacc gacaccgtct ttataggccg
     4861 gacggaatcg accgcacgta tattgctgga agtcaatcgt tcccggaagg ggtgcccgat
     4921 tgacgtgggc atcgaaaata gacaggaaaa taatgacttt ccgacacctt tgattcagat
     4981 aggtgtcttc atcgacctct tcgataccga caacggtacc gtcagccggc gacactaata
     5041 aatggtcatc cgtcggcggt ctgcgccgag gattccggaa aaaatacata aaatacaagg
     5101 ccagcaaagc cggcaggact gccgcatagg cattgatgaa ccagcctgcc gccagggcca
     5161 gtaaaagcgg cacaatgata aatgggtagc cgtcttctac aatataaggt ttgtttaaga
     5221 catcactatt caatttatca cctcaactca ttggcttcgc ttgctgccct ttacagccag
     5281 tacaaaccgc ggcagggcca gcatgcgctt aaagcgtgtc ggctctttaa gaagacgata
     5341 gagccattcc aagcggtttt cctgcatcca tttcggagct cgagctgctt tgccggctaa
     5401 gacgtcaaag gtcccgccga cacccatgca gacgcagggt ccgagttcat acaagtgctc
     5461 gtgtatccat ttctcttgtt tcggcacgcc gagagcgact aaaaggattc gagtcccttt
     5521 gctgccgata tcttcgataa tttgtttttc ttcatcatca gagaagaacc ccgaatgagt
     5581 cccgacgaca ttgagcggcc cgagctgctt ctgcatattg actgcagcct gttcagcaat
     5641 gccgggagca cctccaaaga ggtagacagc cgtttggcgt gcagctgctt cttgcagcag
     5701 actgcacgcc aaatcgacac ctgtaacgcg ttctttaaat ttcttgccct gttgttctgc
     5761 agcccacaag acaccggccc catcgggaac taccaggtcg gcctgctgca ggatctgcgc
     5821 cagttcggaa tcggactgag cccgcatgac catctccgca ttggccgtcg caatggaagc
     5881 agcacggcct tcgtcgatac atttgaaggc cagtgcctgt gcttctttca tggtgacgtt
     5941 gtcgatggga atggacaaga tatcgattat actagtcaac gtatccctcc gccctgctta
     6001 gtttacgctg taattcggag cttctttcgt gatattgaca tcatggggat ggctttcacg
     6061 aagaccggct gcggtaatct ggatgaactg cgtattttca atcatttcct tgatgttgtg
     6121 gcagccgcaa tagcccatgg atgcgcgaag accgccgacc atctgataaa tcgtatcggc
     6181 agcaggtcct ttataaggta cacggccttc aatgccttca ggaacgagct tcttcgaatt
     6241 cgaatcttcc tggaagtaac gatctttact gcccgattcc atggcagcca gggaacccat
     6301 gccgcggtat tctttgtagc ttcggccctg gtaaataacc gtttcgcccg ggctttcttc
     6361 tgtaccggca aggagattgc ccatcatgac gacgttgccg ccggctgcaa tggccttagc
     6421 catatcaccg gagtatttga tgccgccgtc ggcaatgata ggaatgccgt agcgctgtgc
     6481 cacctttgca cattcgtgaa cggccgtaat ctgcggtacg ccgatacctg cgacgatacg
     6541 ggtcgtgcag atggagcccg gtccgatacc gactttaacg gcgtcgacgc cacattcaat
     6601 gagggcttct gtagccgcac cggttgcgac gttgccggca atgaccggaa ggtgcggata
     6661 gagctttttg atttctttca acgtattgag tacgcccaag gaatggccgt gtgccgtatc
     6721 gatgacgacg acgtcgactt tggcagcgac gagagcatcg aggcggtcgg tcatatcatg
     6781 ggtgacaccg acagcggctg cgaccaagag gcggccgttg ccgtctttcg ccgaattcgg
     6841 atatttcgta gctttttcaa tatccttaat cgtaatcaac ccctgcaggt tgccttcttt
     6901 atcgacgagg ggtaattttt caatgcggtg cttgcggaga atttctttcg cttctgccag
     6961 cgacgtgccg accggagccg taatcagatt ttcctgcgtc atgatgtcac cgatacggcg
     7021 gctcatgtct tcttcaaagc gcatatcacg gttggtaatg atcccgacga gtttgccgtc
     7081 gaccgtaata ggtacaccgg agatgcgata tttccccatc aattcattgg catctgccaa
     7141 aagattatcc gggtgaagga aaatgggatc gatgatgatg ccgtgttccg accgttttac
     7201 cttgtcgact tcacgggcct gagctgcaat ggacatattc ttatgaataa cgccaatgcc
     7261 gccttcgcgg gcaacggcaa tagccatagg ggcttccgtt accgtatcca taccagaact
     7321 cataatagga atattcagtt tgatatcgcg ggtcaggttc gttgatacgt ccacttcttt
     7381 cggaagtacg tcagacttag ccggaaccaa gagaacgtca tcaaatgtca aacctctcat
     7441 accaaattta tcagatctca tatttgcacc tctgttttga aaaatataag aaataaaagg
     7501 ggtaaatccc cttaaatagc ttatccctta ttataactga aatctcctgt tctgtacata
     7561 attttagagc aatnnnnnnn nnngcaaagg acttgccaaa acgccgcttc agcgcttctt
     7621 ctgccacctc cggatttccc gaggtctgcc accactgccg tgcttcatcg cgggccgctt
     7681 ccaagataat tatatcgttt atgatgtcgg cagcctttaa gtcgggcaga ccgtgctgga
     7741 gatagccaaa aagctgtccc gagccgcgaa gaagtaaatc cttttccgcc agggtaaagc
     7801 cgtcgtgaat cgtttccatc catttgagcc gctgtttcgt ctcgtcactg ccgcccttgg
     7861 ccatgagaat gcagtaagcc tgcaaatcgc cgcgtccgac acggccgcga agctgatgga
     7921 gctgggccag gccgaagcgc tcagcgccgt cgatgaacat gacggtcgca ttgggaacgt
     7981 tgacgccgac ttcgatgacg ctcgtcgcta ccaagaggtc gatatctccc ttttgaaagg
     8041 cctccatgac ggcctccttt tcttttccgg ccatgcggcc atgaacgagg ccgcacgtgc
     8101 ggttcttaaa gaatttatga cgcagctctt cgtaaaggtc ggtcgcggcc tgaaggtcga
     8161 cggactccga ggcctctacc aagggacaga cgacgtagca ctgctggccg gattccatga
     8221 gcttgtccat gaaggtatag acccgccggc gcagggcttc accgacagcg taggtcttaa
     8281 ccgtctttcg tcccggcggc agttcgcgga tggacgacac gtcgagatcg ccgtacagcg
     8341 acaacgtgag ggtccgggga atgggcgttg ccgtcatgaa taaagcatgg ggctcctgcc
     8401 ctttccgggc cagggccgac cgttgacgaa cgccgaagcg atgctgttca tcagcgacga
     8461 ccaatcccag gctgcgaaag acgacatctt cctggatcaa ggcatgagtt ccgatgagga
     8521 tatcaatcgt accttcttgg aggtcttcta agagctgctg ccgctcttta gccgtcgtcc
     8581 ggccggttaa aagggcgatg ctgacatcga ggccgtcaaa gagccggaga aattcttcat
     8641 aatgctgagc cgccaaaatc cccgtcggcg ccatgagcgc cccttggtag ccgttttcga
     8701 cgattttggc cagggccagg gctgcgacga cggttttccc cgagccgaca tccccttgga
     8761 caaggcgctg catggccact tcactttcca tatcattttg gatatctaaa aaggccctcg
     8821 tctgtccttt ggtcagccta aaaggcaggt gctcatagaa agcgcgaagc agacggccgt
     8881 tggggccgca tttaatgccc tgccgcttcc cttcacggtg acgacgtaat aagaggccga
     8941 gctgnnnnnn nnnntcgaag agttcttcaa aggccagttg gcggcgagcc ccttcttttg
     9001 cctccataga gggtggaaaa tggatcgttt cataggctgc aagggacggc atctgtgaaa
     9061 aatccgaacg gcgccctgcc tcccaaccgg ggaggatgtc caaatgaacc gccgctatat
     9121 cgagtgcccc gcggacggct tttctcacat cctgctgccg caaaccgtcc gtcaggccat
     9181 agacggggac aaacccggag ccttccaccc ttccttcttc cgtatcgggc gaattcatct
     9241 gtctccggcc gtaggcccat tctaatttcc caaaggcggt cagagtcatg ccgaccttaa
     9301 acagccgttt cttgaaggcc tggttgaacc aaaccaactc gacagctccg gttccctcgc
     9361 tgagggcaac tttcaacagc gacactcccc gtctcggccg tgcctccagc accgaagcca
     9421 cggtaccgca taacaagacg gcttccccgg taccgcacgg cgcctggccg attggccaaa
     9481 cgtggctgcg atcttcataa tcgcggggaa aatactgcag gagatcggtc accgtcgtaa
     9541 gatgcagccg ggcaaaaagc gcttccttac gcggtccgat gcctttaata tcacgaatag
     9601 aaaattccat aaaaaaactc cttgtcaaga actttcattt attgtatcat actttgtagg
     9661 ctttttataa ataaaacttg cgttgcccta tgatttatga taagatttat acgttgtact
     9721 gtgatggtgc agggaggtgt aactaatggc aaacttttgc gaagtatgtg gtaaaggaac
     9781 aatgagcggc atgaatgtca gccattccca tgtaaaaacg aaaaaaacct ggaaaccgaa
     9841 tatccagcga gtccgcgcta tcgtcgacgg cgaaattaaa cgagttaacg tctgcactcg
     9901 ttgccttcgt tccggcaaag tccagcgtga tgtttaatga ctatcgaaaa agcgttgcct
     9961 acgaaggcag cgctttttta atgccaaaaa gataccgaaa acgccgccct tcttcacaag
    10021 gaatcgagcc ctcgagacga gtaaaaacaa aaaggattgg tcctcagacc gaatcctttt
    10081 ttattttccc cggaagggct tatccctcat cagggtatag ttccttattt cgtttaacga
    10141 tttccatgac taaattagcc gtttcttcga tagctttcga cgacacgtcg atgatcgggc
    10201 aatggaggcg ccgcataaca cctctggcat attctaattc ttcgttgatc cgctcgatat
    10261 tggcatagct ggcattggca tcgagcccca tactgcgcag ccgttccgaa cgaatgaagt
    10321 tcagtttaaa gggatcgata atcaggccga cgattttacg ggccgggatc tggaacaatt
    10381 caggcggcaa gttgacttcc ggtacgagag gaaggttagc tgccttgacc cgtttgtgtg
    10441 ccaaatacat ggacaagggc gtcttcgagg tccgcgatac cccgacaatg acgatatcgg
    10501 ccttcaaaaa gcccatggga ttcttgccgt catcgtattt tacggcaaat tcaatagctt
    10561 cgacacgctt aaagtattct tcgtcgagct gatgaatcat cccggccttc aacgtcggtt
    10621 ctgtatcggt caatgtgccg acaccggaca gcataggccc gatgatgtcg acgacggtaa
    10681 catccgtatc ggccgtcaag tccttcaatt tgcgccgcaa agccggagaa atgatggtat
    10741 agcaaatcat ggccttatcg gcttctgcct tacggaccaa ttcttcgatc tgctggtccg
    10801 agcgtatata cggtacacgg ataatattaa attcttgttt gtcgtattga cttgccgctg
    10861 cccgggcgac gcgttctgcc gtctcaccca gggagtcgga gcaagcgtaa ataatcggct
    10921 tttccaagat tatctcctcc ctaacccaca ttctacaaac aggcggttaa tattggtctt
    10981 tgaaattctt ccgatgacct gcagttcttt tttatcatct tcatcactgg tgataacgtt
    11041 gacgacaggc agacagtcga tttcatattc atttaaacgc tgggcggcga cgatagcctt
    11101 ttcatcaggc tgtacataga tcaccttact caccggcgtc atgaccatcc tcaccggcag
    11161 cgtcctcaaa tcattgctgc ccatagccgt cttcaacaaa tcctttcgcg atacgacgcc
    11221 ggccaggata ttgtcggtcc cgacgaacac cgtccccaca tcttctgtaa acatggcgat
    11281 aatagcgtca taagcgctcg tatgctcgcc gacgatgacc ggctgagaca ggcaagaatc
    11341 gatggtaata tccgtaatgg cccgcgaaag caggtctttt tccttattgc ctgtaaaaaa
    11401 gtagcctaac ttcggacgcg catcaatcag tcccagcatc gtcaaaatgg ccaggtctga
    11461 gcgcagggcc gcccgtgtca catgaaggcg attggcaata tgctggcctg taatcggact
    11521 gttttccttt acaatctgta aaattttttc ctgacgttcc gataacatag cgtcttttca
    11581 cctcctatgt accaatagca ctctcattcg cttactatca tatcatatat agggataatc
    11641 cgcgatataa agtatacatt atttatagtc acatcatatc gaatagtcct acccgaaaaa
    11701 ggcggtccaa tcatttcgtt ggaccgcctt tcttcggctc acttcgtcac gggacgatta
    11761 ccacgtataa tcttctgtat cagcacggcc ttttccggtc aaggaatcga ggaggattcg
    11821 ctgttcgacc tgggcaatat gcttccgcgt cgagatgacg gcatcgggat tgacggaaat
    11881 ggagtcaatg ccgctcttga ccaagaattc tgcaaaatca ggataaacgc tcggtgcctg
    11941 accgcagata gaaacggtct taccgtcctt atgggccact tcgatgagat ggcggatagc
    12001 ccgtttgacg gccaaattcc gttcatcaaa gagaggcgct acggtatcat tgtcgcggtc
    12061 acaacccatg accagcatcg ttaagtcatt ggaaccgatg gaatagccgt cgacgaactg
    12121 attgaactgg tcagctaaga tgatgttcga cggaatttca gccatgagcc atactttaaa
    12181 gtccggaccg cgatggaggc cttcctgtgc catgaggtcg acaactttct gcatttcatc
    12241 gacacggcgg cagaagggaa tcatgacgtt gaggttcttg aagccatatt cttcacggac
    12301 tttacgaacg gcctgcagtt caagtttaaa ggcttctacg tatttaggat cgtagtagcg
    12361 ggatgcaccg cgccatccga ggagagcgct gctttcttcc ggttcatatt tatcgccgcc
    12421 tttaaggttg cggtattcac tcgatttaaa gtcactcagg cgcagggtta ccggacgcgg
    12481 agccaaggcc tggcagactt tgcggatccc ttcggacaat tggtcgacaa cgtggtccgg
    12541 acggcccgtt tcgatgagat ataagggatg ttcatgaatg aaggtcgtcc aaataaattc
    12601 ttcacgcatg aggccgatgc cgtcacaagg aagatcgccg tatttttcag ccaggtcagg
    12661 attgccgaga ttcatataaa tcttagtgcc cgtaaccggt accgattcgg cagcgacgac
    12721 agtcgtgccg ccagcaccac cggcagcttc tgaaccttgc ggtttgatca agtcggcgac
    12781 gacgccttca tagacaatgc cattggtagc gtcgacagtg atttcagctc cgtcgggaat
    12841 ggccgttgtc gcttcgacgc tgcggctctt ggtcccaacg atacaaggaa tgcccaattc
    12901 gcggctgacg atggaagcat ggcaggtcat gccgccggca tcagtgacga tagctttggc
    12961 tttcttcata g
//
LOCUS       DAWDXZ010000002        11667 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_2194_length_11667_cov_9.511884, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000002 DAWDXZ010000000
VERSION     DAWDXZ010000002.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11667)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2194_length_11667_cov_9.511884"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    988..1022
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8594..8603
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2845 a         2723 c         3210 g         2844 t
ORIGIN      
        1 cgtcttctgc aaaacgcgaa gcgagacggt ctaaaaatgc cgatatatcc tgacagtctg
       61 catagccgaa ttgctgcaaa aatgaagaaa tgtctcgccg ctgtatgtac cttaaaacta
      121 catttggacg gtatacatac agcaaggtgc gcttaggaca ggcatacaag atgcgaaaat
      181 ataatccttt agcattatat ttatgattgt aattttcaat taaatcatca taagtttgac
      241 cgtcctcgag ccgggggaga cagagaaggg atgcctgttt aagtcccgct agagtcggag
      301 aacagtgatt aattagcaca caatctaaat tcatatcggt atgccctcct ttcgctttgt
      361 tgcctttagt atagtttatt tgaaatcgat tgtcaatata aagatgataa ccattttcga
      421 ataattaaac ttttcgcagc gggtgaacct gttcacaatg cctgccgaat ctttcacgag
      481 acttgccaaa tatagtataa taaaagagta ttaaagtaga ggagggtgtg ttatggaagg
      541 cgctagcgct acgaatcatt gtttcggctg cgggcgtgat aacccgattg gtctgaaact
      601 ggatatgcat atagaagacg gccgctgtgt ggcttatttt acaccgaagg cagagcatga
      661 aagctatgga gatcggatgc atggcggtct gaccagtacc ttattggatg aagtcatggg
      721 tgactatgtc tttcgcacgg aggggaaacc ggcctatacg gcacggctcg agatacgctt
      781 tcgcagcgcc atccgcatcg gcgaaacgat taaaatcgaa ggttggccgg aagtacaccg
      841 tcgtcgctta tttatcatga aagggcgtat aacccatgcc gacggcacgc cagctgccga
      901 agctacggca gaattaatgc tggcaaaata ggaggtttta gaatggatag aacggatatg
      961 acccaccgca tgtatgacat taaaggannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnccgttagt ggctaagaaa tttgaagccc gtggctttga ggcgacatac tgtcccacgg
     1081 ttgaagcggc attgaagcaa gcgatttctt atattccgga aacggatgtc gtctcttggg
     1141 gcggttcggc ttcgatcaat gaaatcggcc tgcgtcccta tgtcttggaa cacaatcagg
     1201 tcatcgaccg cgactctgcc aagacgccgg aagaacgggt tgaattgatg cgtaaggcct
     1261 tgctctgcga taccttcatc atggggacca acgcagccat tcaggatggt cagctcatca
     1321 acatcgacgg caacggcaac cgtgtggcag ctctttgcta cggcccgaaa caggtcatcg
     1381 tcatcgtcgg catgaataaa gtcgttccga cggttgaaat cggtattgaa cgggcccata
     1441 cggtagcgtc gccgaaaaat atcggccgct ttcagaatgt cgggacgccc tgtgctaaaa
     1501 cgggcatgtg tgctcactgt acgacaccgg aaagcatttg tgctcaagtc gttcggacgc
     1561 gggtctgtaa gccccaggga cgaattaaag ttatcttagt tggagaagat ctgggctttt
     1621 aagccttaat ttacctatcg aggggagatt tatatgagaa caggaacggg tgtcattgaa
     1681 gaggtttgtg ccgacggatt ggtcaagatc cagaccaatc gcagtaatct gtactcgccg
     1741 tgcagtgaca gcatgtgcgc tgacaacgtc gtcatcgacg ccgtcaatcc cattggtgcc
     1801 aagaaaggcc agtttgtaca gtttaatatt cccgatgccg gcatgggggt caagggcatc
     1861 gtctgtttcg gtgtgccgct gctgctcatc atcgtcttcg gcatcatcgg ctacatcttg
     1921 ggcggtcagg taaaggcgac gccggaaatg acggcctttg ccggcatgat cattggcggt
     1981 attatcggct gccttattct caaaggatac gaaaagaaga tcaataacaa tcaaaacaag
     2041 gcgacgatta cgggcatcat tgcctgattt cgccaggact atctgtttga tagccaaatg
     2101 caaaaaggct gcacaccgtg tgatgtgcag ccttttacgt atgaagttat acgatatcga
     2161 tttgacaatt ctgcataatc ttcaaagcca tttcatgggc ctccggtgta gagccggcgc
     2221 agcaggagct gtcgacgacg atttcttttt ccggataatg ggccttcaat aaaatcgcat
     2281 tggaaacgac acaaatatcg gtacaaagcc ccatgaggac gagtcggtca taatctttga
     2341 cttcatcgac caaggccggg cagccgaaag acggcttttc aaaaatctct gccatgagcg
     2401 tgtaaggcag gagctgtttg cagatgcgcc agccccgcgt atccttgatg cagtgggcaa
     2461 tggggagttt ttgaccttcc cgggtttcca gataatcact gtcatgggta tcctgcgtaa
     2521 aaatcagatc cgtctcttgg tcggtatctt ttttgctggc aacgattttt gcttcgacat
     2581 ggggaacgat ggcttgagcc tcaggcgtgc ctaaggagcc gtcgataaaa tcattttgca
     2641 tgtcgatgat gatatatgct tgcgtcatac agccctcccc tattctttag gaacgaagtt
     2701 ctttcaagta ttccgggacg tccgggcagg tcatgagacc tgacatcaag gctacgccgg
     2761 taacttggga gtcctgaagc tgggatactg tcgtggggtt tacgccgcct aaggcgtaga
     2821 cgggaatgtt tacggcgctg caaattgcct ttaccgtatc gactccgatc ggtttatccg
     2881 ccggtttgca gtctgtcggg aagacgtggc tggccatgat atacgtggcg ccaagttctt
     2941 cggctcggac ggcttcttct acggtatgga cagaaacgcc aagggtcgtc atataataca
     3001 ataaggaaga gtggctttca aggtatgaca aggagcattg gaaacgagga atgtgaagtc
     3061 gaatggcgac tttgccaaaa tgttgaagga tacacgtctt ttggtgaatg ctgcagaggc
     3121 gaagtgcccg tttggcatag tccgtgtagt catcttcaga gagcgttttt tcacgaagga
     3181 cgatgaagtc tacgtcggat gcagcaattt gttcgagctg gtcccagtag tttgtcgccg
     3241 caagttcgtg gtttgtaatg gcagctactt ttaacacaat tatcacctga atattagagt
     3301 attttatact acaaatatac agtaatctca tactaacgtc aatgattata ccaatagaat
     3361 ataatagggc cctcctatga gttaaacgct ttagaacatg cgggtttacg acaaaagcag
     3421 gtcggggtgg attaggtcct gacctgcttt tgtcatagtt ctatataggt atttaatagt
     3481 ggatatcaga taagtattac aaataaatat actcgctcgt gaggggctgg aggcccatgt
     3541 tcttgatgtc agcgtacatc tggtcgaagg aacggccgtc ggtgatttca aactgttcat
     3601 cacccatttc cgacccgtct tgatgcaggt ggccgccgat gccgacgcag acactggccg
     3661 ataccttggt ggctgcaatc ttcatgatgg cattgcggaa ggaagcactt tcacggctcg
     3721 atacgacgat gttggcgtaa ggcaggaata agcgataggc acagatgatt tgcgtcagct
     3781 tccgttcatc gacatctttt ggattgattt tgtcgttgtt gatgatcggg cagagacgcg
     3841 gacaggacag ggagatttca gcgtgagggt acttgcgctg catgagccag gcgtgcatgc
     3901 ccgttgccag ggcgtctttc tggtagtcgt cgaggcctaa gagggctgcg aagccgacgc
     3961 cgcgcatgcc gccgagaata gcccgttcct gagaatagaa gcggtacggg aagattcgtt
     4021 tatggccggc caagtggagc gtttcgtatt tgtcggaatt gtacgtttcc tggaatacag
     4081 tgacatagtc ggcgccgcat tcgtggagat aggtatagtc cttggtgttg gtcgggtata
     4141 tttccatgtt gatgacacgg aagtactggc gagcgatttt gacggcgttg ccgatgtact
     4201 tgatgtcgct catcttcgga ctttcaccgg tcagcatgat gacttcttca atgcccgagt
     4261 cggcgatatt cttgcattct tggtgaagtt cctcgtcgtt cagacgggcc cgttttattt
     4321 tgttgtgcga gttgaatccg cagtagatgc agtgattgtc gcagtagttg gcaaaataaa
     4381 tcggcgtcat gatggaaatg ttgttgccga agtggcggcg cgtgaccaaa tgggcttttt
     4441 gagccatttc ttcgaggaac ggggcagctg ccgtcgataa gagggccatg aagtcccgcg
     4501 ggtccaagcg ttctttcgat aaggccatgc ggacgtcttt agcggtaaag tcttcattgt
     4561 taaatgaatc gtgaatggcg acgacttggt cgagcatgtc ggaatcgagg atttccatgc
     4621 ccggcaggta ggtcatatgg tcgatacgtt tttcttctgt catgatcgca ttcctcctca
     4681 ggcacctaag aaaccggtaa gcggatctga agccgatgcc gtttcacgga tgcggccgag
     4741 gccggacaga taggcgttgc ggccggcgcg aatggcttcg ccgaaggcct tggccatgag
     4801 aggaaggtcg ccggccgttg cgatagccgt attggccatg atagcagcgg cacccatttc
     4861 catggcttca caggcttggg acggacggcc gatgccggcg tcgacgatga tgggcagatc
     4921 gacttcgtcg atgagaatct ggatgaagtc ctttgtcgat aagcctttat tggtgccaat
     4981 cggagaggct aagggcatga tggcagcggc accggcatcg acgagggccc gggctgcgta
     5041 gaggtcagga tacatgtagg gcaggacgac gaaaccttct tttgcgagga tttccgtagc
     5101 cttgatcgtt tcgttgttgt cgggcaggag gtatttggag tcgcgcatga cttcgatttt
     5161 gatgaagtcg ccgcagccga tttcccgcga taaacgagcg atgcggacgg cttcttcagc
     5221 cgttctggcg ctggacgtat tgggcagcag ggtgacgtct ttggggatga agtctaagat
     5281 attgtctttt tcaccggctt cgacgcggcg cagggccatg gtgacgattt gtgcggcgcc
     5341 ttgttcgact gccgccttga tgaggtcgac agaaaatttg ccggagccta agatgaaacg
     5401 ggacgtgaat tcatggccgc caaggaccag tgtatctttc ttttccattg ggagacactc
     5461 cttttatatt gtgtaggttt tctgcttagt cagaagggtt agcccccgcc gacaaagtgg
     5521 acgatttcca tggaatcgcc gtcttttaag gtcaccgtgt cataagcttt acgcggtacg
     5581 atcgtgccgt tcagttcgat ggcgatgcgg tctgtgcgat aagcctggtc ggtaagataa
     5641 gacagcaggt tctggccgtc tgctgcgatg tccttgccgt tgatgcgtat catgctggcc
     5701 ctcctttcta tgcattaaaa aaaggccgca cgaagactta cacccaaggt ggtgtacccc
     5761 ttcgtgcggc cttttgtttc ggtctcactc tacctttgat tatagacgat tgcctgaaaa
     5821 agtcaatcgt tttatgccaa tcagtaccaa acggtatccc tgaccgatat ggtcgaaata
     5881 gggcggactg gcggcgtgac agggaacata gtataataaa attataaact cacgagaggt
     5941 gatgactgtc atgaatgaac ggatggattg gacggatatt gcccgctatt tttacttgcc
     6001 tgatgagcgg ctgcaggcga tttcccgtga tttctcggcc gatatcgaca gggccttggc
     6061 cggtgaggcc ggcgccacgg tttcggctga aaaatcgtat gcatccctgc cggaagggga
     6121 tgaaaagggc gtatttttgg cactggactt tggcgggact aatgttcgag cctcgcggat
     6181 tcgcctcttg ggacgacatt gctatatcgt cgaaaaaaag gtatcccgac cgctgaaagt
     6241 ggccggtgag tacgattact tgtcctcttc gacgacggcg tcagaactct ttgatttctt
     6301 ggcccgtctt gtcggcaaag tagctcgcag cggccagtct tatcgactgg ggcacgcctt
     6361 ttctttcgcc ttgaagcgga ccggtatcga agacggccgc ctcctctcct ggtctaagga
     6421 aattgccgtt tccggggtcg aaggtgagaa cgtcaacgcc ttgctgaaag cggcgctccg
     6481 gcggcaggga ctcagcgaca tcgagcccgt ggcccttgta aatgatacga cggccctctt
     6541 gctgtcggcg tcttatacga tggagaaggt ccgtatcggt atggtctgcg gtaccgggtt
     6601 taatgcctgt tattatgagc cggaatggaa tatgatcgtt aacttagagg ccggcgatta
     6661 caacggcgtt acgggtcatc actgggatga ggctgtcgat gccggatcgt ctcagccggg
     6721 gctgcataaa ctggaaaaga tgatgagcgg cgcttatgcc tcggaactct ttcgccaaac
     6781 gctgttgacc tactttgaca ctcaggaact gcctcctttt acgacggaag caatgaatga
     6841 tatcattggg aacgatgatg atcaacaggg ccggcttacg atggggcggc tgtggaatcg
     6901 catcgtctgc ttggaagatg tgcgccccat aaggcatatc ggagcggcta tttttgtgcg
     6961 ttcagctcag cttgtcggcg ccgtcggctg tggtattctg catcatttat ataaagagct
     7021 gcccattccg agtcagacca ttgccattga cggaggtgtt ttaaaacacg ttcgaggtgc
     7081 cttggttatg atggaagatg ccatgcaggc ctgccaaaat gaaggtccta atcgggaaaa
     7141 acagatttcc gtcgatccga ttctcatcca agatggggtc tccgtcggtg ctgcgattgc
     7201 ggctgctatg agcataagat agtttttata agaaaatata tggatttgcc aaggaatcgc
     7261 cgggtatccc ccttttcagg cggcgcattt ttcggtacaa tataatatta cagagacaac
     7321 catagacatg gcgacgccac agaggggaga aggggcctta ggccggatgc aatgctgcaa
     7381 ctcgacaaat ctatcagcaa accattttat gtacaaattt acgaatatta tcgccaggaa
     7441 atagaatcga gccgcatggt agccggtatg cgtctcgatt ctgtgcgtga attggctcaa
     7501 gctgccggca tcagcaagat gaccgtggaa aaggcctact accaattggc cagtgaaggc
     7561 tatatcctgc gccggcacaa agcacgctat gaagtagcct ccttgggccg gcgggaacaa
     7621 ctgccggaag aaggccgtcc aatgcgggtg caggaaatcg tacatcagcc ggtgtatgac
     7681 tatgacttcg ccagcggcga catggccttg gagcgattcc ccctcgatat ttggcgaaag
     7741 tacatgaatc gtgttttgtc agagccggat cgcctgttgg cggccaatga cgaccagggc
     7801 gtccctgatc tgcgccgtgc tctgagccgt tacgtctatg aaacacgcgg tgtccatgca
     7861 gcgccttcac agattgtcat cggcgccggt acgatttccc tgctgggcat attgacgcac
     7921 ttactgcgct gtaaatacga ctgcatcggc gttgaagagc caggattccg actggggcgg
     7981 gaaatctttc gcaacagcgg ctattccatc gttcccattc ctattaaggg cggtctcctt
     8041 caggtcgatg atctggagca aagtaacgtt cgcttggtct acgtcagtcc gtcccatcag
     8101 tttccgacgg gaacggtcat gccggccggc attcgccatc gtctgctcca atgggcagga
     8161 gagactgacg gactcattat cgaagacgac tacgacagcg agcttcgcta ttacggccgc
     8221 ccggtaccgg cacttcaggg actggaccgc agtggccgcg tcatctatat gggggccctt
     8281 tcgaaggtat tgcctttttt tgtccgcctg agttatatgg tgcttccgcc ggaactcatg
     8341 ccgttatacc atgcccggcg aggtctcttt cggcagggcg cttctgtgcc ggaacagtgc
     8401 gtgttggcgg aatatatcca gagcggcgaa ctttcacgcc aagtccgccg gctgcgcaag
     8461 gattatcagg aaaaaggcga attcttgagg aaactcctcg tggaggcctt tggccgggaa
     8521 atcgtcgtcg gccaactcgt ttccggcatc tactgtcacg tccgcctgca cagctccttg
     8581 tcagaatcgg attnnnnnnn nnnctgccgc gtcttatcca tgcagtcttt ttatgaaaca
     8641 ccctcacagg aaaccgacaa tgagtttctc ctgtcatttt caaaaattcc cagctgtaag
     8701 ctccgtgatg ccgttgcggc cctgcatgca gcatggtcag agaaggaagg ataataatgg
     8761 cgaaaaattt gcggtttatt caatgcggag atcttcattt aggaagcccg ttccataatc
     8821 tggccgccat cgatgatcgc tggcagcgta tcatcggcaa agctccggtc cgggcctttc
     8881 aaaaaatcgt acagctggct atcgataaac aagttcacgc cgttttgatt acgggtgatg
     8941 tctataccag tgccaatcac aatctcacgg ctcagctcga ttacgtgcgg ctgcttcata
     9001 aattggccca gcatcaaatt caggtctttg ccgtccttgg caatcacgat cctctggaag
     9061 cttggaaggc aaaaatacct ttcccgccca acgttcatat cttttcggca gaggctgtcg
     9121 aacgggtacc gtttacggcc aaagctatgc taaaggcgaa cagcgggaaa atctggcctg
     9181 gaagttcaac cgtgccgccg gagatacctt ttctatcggc ctgctccaca cccaagtcgg
     9241 cagtcgtgag tcgacgtatg ctccgtgcac ccttgacgat ttaaaagaat gcggcatgga
     9301 ttattgggcc ttgggtcata ttcataaaca cgaaatttta tgtgaaaaac cgtatatcgt
     9361 ctatgccggc aatccccaag ggctggactg cacggaaagc ggtcctcggg gctgctatta
     9421 cgttgaagtc ggcccttatg gaacgacgaa tatcgagttc gtcgatacca gtatcgtccg
     9481 ctgggaaagc attgatattg ccatcgacgg cctcgagtcc gtatcggaac tgcgcggtgc
     9541 cgtgcgcttt gctaaagaaa aagtgcgccg tgatatcggg aaaccaacgt tcttgaccgt
     9601 taattttacg ggcagcggca gtatgtacca tgtcatcaac aatccggaag cgacacagta
     9661 ctggctggaa agctggcgtg aagaagaaca gggaaaatat gccttcgtca tggtcgaacg
     9721 gctgcgcaac ctggcccgtc cgaaaatcaa tatggatgaa cggagtaaat tgcccgactc
     9781 cattggcgat tatttaaatg ttttcgacaa tttagaaaaa ttggagcctg ctgaaaaagc
     9841 aaaggccctg cgggatatcc tcatgaatcg gcccgagttc gaacgcctcg gtgcgtatgg
     9901 ccgggcgatt tctgatgacc gcctgctgga aaactttgaa aaagccaagt ggctgggcat
     9961 gcagcgcttg ctggaacata gaagaggata atgtcatgaa gattatacag atggaattag
    10021 ctgatttcgg gatttatcat ggtgtcaaat ggaatccccc ggaaagcgga ctgatcgtca
    10081 tgcacggccg taatgaaagt gggaagacga cccttatgaa gtatgttcgc tccatgttct
    10141 tcggctactt gcggggcgac tggcacggct atttcggcag tatggatatc cgccgggaag
    10201 acggaaaaga ataccggatc gtccgcaatg aaaaagaata taccttatcg aacggcgacg
    10261 aaaaccttca ggaagaaccg gccgatttgt ggtggcacgg gctggatcgt cagacctacg
    10321 ataagatttt cgccatgggt cttgaggacc ttcagggctt taaaatcctg tccaacgaag
    10381 ccgttcgcaa tcactttttc agcattgaag gcggcgtcgc catggggctg gcccgcagtg
    10441 aagtggccaa agagatgggg gaacttctgg tcgcatctcc ccaggggaaa aagcccatca
    10501 atgccctcct caatgaacag aaagacttcg accgccggat cagcagcctg gcctttgatg
    10561 aagatgaatt cgcgaactta caggaaacgg agcggacgac acatgaaatc gaaaaccgca
    10621 tccgtctcga tattgaagaa tcaaagcagc agatcgagca gatttccatg cccatcgcag
    10681 cttgggacgt ctataaacgt ggtcaggatg ctctgcagca gatgcagaat ttagctgacg
    10741 tatcgcagtt tcccttagat ggcgctcaaa aatgggccga tatggatcaa aagatcaagg
    10801 aaatcgacga acagattaag cacttgaaag aaatgaacca aaagggaccg gcttttcaag
    10861 aatcatggaa gcgctggctc gtctgcggtc ctcaaattga ggatatttac cgcagtattc
    10921 cggcttggaa gcaaggcctt gatgaattaa aagaacacga agataaagaa atagactggg
    10981 attttgaaaa aaacaaacag gcagaggtct tgaaagggtg gactgttacc ggtgaaattc
    11041 cggaaacggt cgactggccg cgcggaatgg ctgcggcttc aacccttgaa cacagccgtc
    11101 aggaaatgga aaagtggcag gctgccaagc ccaagaacgt ctcgtcgccg gaacttgatg
    11161 aagaagatac ggaaaagtcg aaagacgagt ggaatactgt tgggacagcc gtatcagcca
    11221 ttcagtcggc cgttacggaa cggcagaccc tgcaagaaca gctcgattgg ctaaaaaatg
    11281 gggctacggg cccgtcgaag gcctttatcg tattaggtgt gctctttctc ttagccgcgg
    11341 tcgcctgcgt cgctgcggta tatctggcag actttgactc aaccctcgga ctgggtgccg
    11401 gggcggcctg tgccgtcatc gccgtcatct ccctggcccg tcaaaaaacg gggaccgatc
    11461 gtattccccg taaaattgct gaaatagaga atcaattagg gaagcttaat cagcagataa
    11521 gcgacctttc aaaagacgct gaaatcaact tagatgtgga ggccgccaac gatcgctggc
    11581 agcaggaatt agacggcgtc cggaaacagt acatggactg gcagacgaag gaatcaaaga
    11641 atgcctggca gaaagaacaa aaagtga
//
LOCUS       DAWDXZ010000003        11202 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_2271_length_11202_cov_10.100745, whole genome shotgun
            sequence.
ACCESSION   DAWDXZ010000003 DAWDXZ010000000
VERSION     DAWDXZ010000003.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 11202)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11202)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11202
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2271_length_11202_cov_10.100745"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2289..2298
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2950 a         2534 c         2778 g         2930 t
ORIGIN      
        1 gaatcgcatt gcctcccatg aacaagctct tctttccatt ttgcttgatg gaactgagga
       61 tatgaagggc ctacgccaga tgaaaggtgt aacggttaag atggatgatc ctgatcttac
      121 gaaacgtgac cttattacag ggattgagtt cgataatatg tcagcagata aggccagaga
      181 agaaatggaa aagcgtggtg tcgttgcctt tgaacgcctt gcctccagta tttattccgg
      241 gcgcatgttg aaacagtttg attcaccagg agttttgcgt atttcacctc ttcatgttaa
      301 cagtgctgat gatatcgtaa ctttcttaaa agttgccgaa gaagtggctg ctttatagct
      361 ctgatattgt aattatatag aagggagcaa tccaatatgt tactcaaaaa actagggtta
      421 ggtacccgca ttctcattgg tatcgttatc ggttttatta tcggctttgc ttctcctgat
      481 ctaacaaaaa tgttgtcccc tttaggtgaa gtattccttc gcatgttgaa gatgcttatt
      541 gtccctttgg tctttttcag tattaccagt ggtgtctgta agatggggga tgtcaagcaa
      601 ttagtcagta tcggacttcg atttgttgct tatatcttaa tcacatccgg gttagcagct
      661 gctttaggga cgtttgttgg aatgttctta ggcattggtt cgggaaccac cgagtttctc
      721 caggctgggg ctaatgtcaa aaatgttgac tataatttta ttaataatgt agttagttgg
      781 tttcctgcga atgtgttcga gtcttttgtc aaacaagata tgttgcaaat tatcgttttt
      841 tccttatttt taggggttgc aatcttattc ctcagcaaac aggttaatga ttttgtaaaa
      901 atcattgacc agggcgctct cgtcatgttg aaaattactg attttgttat ggcattttct
      961 cccataggta tcgcctccct catggctacg atggttacga ctatcagtgg ggctacggta
     1021 aaggatgtaa tcgctctgat tgccgctgat tacattacct gcatcatttt cttcctaatc
     1081 atgtatccgg ccatgttgaa aggtcttgcc ggattaggcc ctatccgctt tttccgtaag
     1141 atttcgcagc ctatgatagt tgccattact acgacatcgt cggcagctac cttgccggta
     1201 tctcttaaaa ctgcagggca aaaattgggc ttgccggaaa acgtgtatgg atttacactg
     1261 cctctgggta atacttgcgg gatgaatggt tttgctgtat ttattggtct ggcatgcatc
     1321 ttttgtgcca atatttatgg ggttgaaatt acttttgcta aattagttct ctacatattc
     1381 ttaggaatta tcctttctgt tggcgctgct ggggtaaaag gggctggtat cgtaatgagt
     1441 accgtactct tacaggcgat tggtatgcct ctcgatgtta tccccatctt tgcagctatt
     1501 tggccggtca ttgatccgcc gcacaccttg ttgaataata cgagtgatct cgtcggtaca
     1561 gcgattattg gcaaacgctt aggcaagatg gacatggaca tttatgaagg cagaaaagta
     1621 attacggatg aaacgtaaag tagtgtatta gaagagagcc atggatttgt gtttgtacat
     1681 gaatccatgg ctttttatct ttctatttag ttttctgatt ccgtactatc tgtcatctta
     1741 tttataagcc ggttcccatt tgtaggtttt gatgcaggct tcgggatcgg agacgtcgtt
     1801 tctattcaag ccttcttttc cggcttcttt agctacggcg atggcgacgg tttctgaaaa
     1861 ttggtcgagt ttggttaccg gcggcaggac ggcggcgccc ggtttatttg catctacgat
     1921 gccgccgatg gcgtgggcgg cgacggagat cattttatcg gtcagaagtt tggcccctga
     1981 ggcgatgaca ccaaggccca gacccggata gatgagggcg ttgttggcct ggccgatgtc
     2041 gtaggtgacg ccgttatagg tgacgggatc ggtcgggatg cccgttgcca ctaaggcttt
     2101 gccgtcagtc cagcggatga ggtcttcggc cttggcttcg gcaagttctg tcgggttact
     2161 gagggggaag atcatgggcc gatcggcttt tgaggccatt tcttttacga cggcttcggt
     2221 aaaggcaccg ctcttggtcg acgtgccgac gaggatggtc ggatgaacgg ccttgacggc
     2281 agcctctann nnnnnnnnat cgaataagag gccctctttg tcgacgaggt aaaaacggct
     2341 cttggcatca tcttctgaca ggccctgatc gatcatttcc tgataaatcc gtttggcaat
     2401 gccgcagcct gccgtgccgg caccgaagca catgtaacgc tggtcggcca gtttttcgcc
     2461 ggtaatggcc agggcgccga ggacgccgga caaggtgatg atgcccgttc cttcgatgtc
     2521 gtcgttgaag acgggatagg tatcgacgta tttttggagg aggacggcag catggccgcg
     2581 gccgaagtct tcaaagtgga ggtagaggcg ggggaacatc gattcaactt gttttacgaa
     2641 tttatcgacg aaggcatagt acgtgtcatc gtcgactcgt ttatggcgca ggccgaaata
     2701 aaggggatcg tcaaggaggc tttgacggtt ggtaccggca tcgatgacga cgggtaggac
     2761 cttggccggg tcgatgccgg ccgctcccgt atagaccatg agtttccccg tcgagatacc
     2821 gacgccgttg gtgccccagt cgccgattcc taagatggct tcggcatcgg tgacgacgat
     2881 gagttcgata tcgcggccca tggcggtatt tttcagggac tcttcgattt gttcgggatg
     2941 gtctacggaa aggtaggctg cattttgcgg ggttatgaaa aactggctgt attcgcggat
     3001 gctttcggca atgcccgggt cgtagatgat cggcatgaat tcagcgatgt gctgtttgaa
     3061 gacgtagtaa aagagcgtcc gattgcggct gaaaatattc atgaggtaat ggcgcttttc
     3121 gctgacgttg ggcttcgtct gaaaattgcc gtatacctgc tgtgcctgga cttcgatcgt
     3181 ctgctctgtc ggcggcaaga gaccgataag gccgagttca gctctttctt ccatggaaaa
     3241 tgccgtaccc ttattgagga agggatcatt taataattca taaccttttt tcataggcct
     3301 actcctttca gaaacgataa attttttgat ttttttcatt gtatcatagc ggataaaaat
     3361 tggacagtat catatctatt tgttatagca tttttttagg ggaatgcagg aaatgcccat
     3421 ctttgctata atttaggagt agggaaagga gtgtttctat catggatttg accaagacca
     3481 gccgctttat cagcctcgtg ttgcggcata aaccctgggc ggccgatgtc cgactcgacg
     3541 agcatggctg ggccgatgtg gatgaactca tacagggcgt ggggaaaaaa tatcctctcg
     3601 accggcagat gttagaagaa atcgtcagga cggacaacaa gcagcggtat tctttcaacg
     3661 aagacaagac taagatacgg gccaatcaag gccattccat cgatgtcgac gtcgagcttc
     3721 ccatcgtgac gccgccggac atcctctatc acgggacggg tgaaaaatac gccgaggcta
     3781 tcgagcgaga aggcttggta ccgaagtcac ggctctacgt ccacctttcg ggcgacgtga
     3841 agacggcgga aaacgtcggc cgccgccatg gaagacccgt catcttttgc gtgagcagcg
     3901 gcgacatgca tcgaaaaggc cttgtctttt accgctcggt aaacggtgtc tggctgacga
     3961 aacgggtaga gccacagttt ttgacgcggt attaacgctc cttaaaacgt aatccgaaat
     4021 atcccatggg aagggccgta cccgtgaagg agaacgtttc gaggcgtcgc tcttttagca
     4081 tctgcattat ctcctcggtt tcatctaaaa tgcgggcagg atactccgac ctcttaggtc
     4141 ggggaggaat gcccccctca cctcactttc taaaaatact atgatatcag caaaatatat
     4201 tgtataatat ttttgatgaa agtaatataa ctttagaaag gcaggtgata aaatgtccga
     4261 cctaaccaaa actataaaac ttcgcatcaa tatttctcca gaacaggaac tccttcttcg
     4321 acagatgact gaacaatatc gtcaggcttg taactttgtg tcaaagcata tttttgataa
     4381 tcaatttaat ttggcttacc aaagccttaa tagagagtta tacagtaacc ttcgtagtca
     4441 gtttggttta aagtcacagc tagcacagtc tgcaatcaag acaactattg cccgctataa
     4501 gacagtcaag caacagcttt tccaaaatcc atacaaatac aaggatgaaa atggcaactg
     4561 gaaatatagt accaagacac ttgagtggct ttggaaacct gtattgttca gccgtccgca
     4621 agctgattta gttcgtaatc gtgactatag ctttgttgat gatgggaaaa cactgtccat
     4681 taataccctt aacaaacgaa cgaaatgtac atttcgaggt gagcatttcg ccgaatatct
     4741 tgacggttcg tggagccttg gaacggcaaa actagttgaa ctcaaaggag tatggtatct
     4801 gcatattcct gctactaagg ttgtcgaaga gtttcagaca gagaacgtcc gccacgttgt
     4861 cggtattgac cgtggattgc gttttttaac tgtcagctac gatggacagg gcaaaactga
     4921 atttatttct gggcgaaagg ttgccataaa acgacataaa tttcaggaag ttcgttgcca
     4981 gcttcaatcc aaaggcacga aatcagcaaa gcgtaaacta aaagctattt caggacgaga
     5041 gaaccgttgg atgtctgatg taaatcatca gatttctaag acactcgttc agaaatatgg
     5101 taaagatacg ctatttgtac ttgaagattt aacaggcgtg agtttcgagg aatcgaatct
     5161 atccagaact gcaaaacaga aatacgatct acgaagctgg tcattttacc aattagaaca
     5221 atttctaact tacaaagcgc acgaaaatcg ttcagaagtg ctaaaagttt cagcaaaata
     5281 cacttcacag cgttgtccaa agtgtggaac tatccacaaa gaaaaccgtg accatcacag
     5341 acatctatat agttgtcagt gtggatatcg gtccaacgat gatcgtatcg gagctatgaa
     5401 tattcaactt cttggtacac tgtggatttc tggagacaac aaccctcgat acgaaagaat
     5461 aacaactgcc tcggagtaaa ctccttggca aaacgggcat tgtcaactgc ccgatgatgt
     5521 aggcggaatt agggagataa tatcgctaat acctatgtaa ctccgactta caagctccca
     5581 cctcaaacac cgtaggtgtt aggtggtgag cagttgactt aaaattgcaa aatcgtcaac
     5641 gagtctgttg gtcaaatcat ttgactaatg ggctcctttt gttttatagt taaatagtaa
     5701 aaagttttga ctaattatat cccgatgttg gactcatcca ctcatattta aaatcccggg
     5761 tgatgaaagg cgtcgggata gcttggaaca taagggggga atgtcatgaa agcggtcgtc
     5821 atctatgaag ccggcggcgt cgacaagctc atctatcagg acgtcgatac gccgacggta
     5881 aaaccgggct ggtccttggt gaaggtaaag ggctttggca tcaatcgctc ggaaatattc
     5941 acccgagagg gctattcgga atcggtgcac tttccgcgga ttttgggcat tgaatgtgtc
     6001 ggcatcgtca ccgacagcac ggaaccggaa aggattcccg aagggacgaa agtcgtgtct
     6061 attatggggg aaatggggcg ggcctttgac ggctcttacg ctgaatacgt tcttttgccg
     6121 aacgaacaga tttacaaggt gcagaccgac ttagattggc cgaccatggc cgccgttccg
     6181 gaaacgtacc acacggcttt tggaaccttg aaaaatttga agatcgaaga ctccgatgcc
     6241 gtcctcgtcc gcggtgcttc aagcggcgta ggcgtggcct ttgccaaact cctgaaaggg
     6301 aaatacccaa aggcgcactt gtacggatcg acgcgcaatg ccggcaagac cgaccggctg
     6361 atggaagccg gatttgacga agtcatcgtc gaccatagcg gttgcctcga tacggaatta
     6421 tcctttgata aaattgccga cttagtcggt ccatccgcca tggccgacag tttggcccat
     6481 ctgaaagatg acggaattct ctgcaatacc ggcctcttgg gcggaaaatg gaccctcgac
     6541 gatttcgacg tcattatgga attggggagc ggcaagtaca ttacgggctt ctattccgga
     6601 ctcgtcgatg aaggggcttt gcaggccttg ttcgacatga ttgcgaaata ccatatcgac
     6661 gcggcgccgg agaaaatctt ctccttacgg gacattcgag aggctcaggc ttatttggac
     6721 agtgccaaaa gtttcggtaa ggtggtggta atcaatgact gacatttatc aacagacgaa
     6781 ggcctattct cggattcggg accatcaact gtcgattttt gagaattacg ccagaaaacg
     6841 gggccttcag agcaagtcca tgctcatctt gatgtggatt tactacaatc ccgaagggat
     6901 tccccaaaat gagattgccc aaaagaccta ttcgacgaag caagtcgtca atgcgacgat
     6961 caagtctttt aaagaaaagg gctacctccg ttttgaagaa aatcccggcg acaagcgcat
     7021 caaacgaatc aaactgactg aagaagggcg agcttacgcg gcgtcggtcc tggacttatt
     7081 ggaagaagcg gaaatgctgg ccatgagtga gctcacacca gatgagcgag atcttttatt
     7141 acgcgttagt cgtaagttta atgatgcact ggcaaggaat ataggtgact accatgattg
     7201 aatttaaaaa tgtgagtaag atttacgaca agaaaccggc cttacagcac atgagcttaa
     7261 ccattaagag cggcgagttc tttgtcatcg tcgggaccag cggcagcggc aagacgacga
     7321 ccctaaagat gatcaaccgc ctcttgacgc cgacggaagg gacggtcctc atcgatggga
     7381 gcgacatcag tcagctcgag ccgagaaagc tgcgcatgaa catcggctac gtcctccaga
     7441 ccggggccct ctttcccaat ttgacggtct atgaaaacat cgagatcatc ccggaaatga
     7501 agggctggcc caaggcagag cggcagaaga caatccgcgc ctatctgtcc ttggtcggcc
     7561 tcgatgccga tcagtatatg aaccgctatc cccgtgacct ttccggcggc gaacagcagc
     7621 gcgtcggcat cctgcgggcc atcgtggcca aaccggacat cctcctcatg gatgaacctt
     7681 tcagcgccct cgatcccatc agccgcaatc aattgcagga gctcatcaag cacctccacg
     7741 aaacctttaa aatgaccatc gtctttgtca cccacgatat ggccgaagcc gtaaaactcg
     7801 gcgaccgcct ctgcgtcatg agcagcggtg aagtcgtaca gctcgacacg ccgcaggcgg
     7861 tcaaggacca tccggcccat gaagttgtca aacgtttttt ctacgagggg taaactatgg
     7921 aaaacttaat gagtacattt agtgaacgac agggcgaatt ggtacaatta ttgttggaac
     7981 acgtgcagat ttcgatgatt gccctctttg tggccatctt actggccgtg ccctttgcca
     8041 tctttgtcgt aaagtataag aaagcctcgg aagtaaccct gcagctagca ggaattttac
     8101 agaccatccc gtccctggca ctgttggggc tgttcattcc tttcctggga atcggcaagg
     8161 tgccggccat cgtggccttg gtcatctacg gcctgtttcc cattttgcag aacactatta
     8221 cgggccttca gggcatcgat cccctccttc aggaagcggc cgaagccttc ggcatgagcc
     8281 gtatggaaaa gctgaaaaaa tttgaactcg tcctggccat gccgtctatt atcggcggca
     8341 tccgcacgtc gtcggtcatg ctcatcggga cggcgaccct cggagctttg atcggtgccg
     8401 ggggattggg gaccttcatt ctcctcggca tcgatcggaa caattcggcc ctcatcctca
     8461 tcggggccat tacggcggcc ttattggctg tcgtcgtcag cgccctcatc aagttcttag
     8521 aaaaacgctc gttaaaagtc atcggcctgt ccttcgtgac ggccttggcc ctcttggtct
     8581 tatcgacagt caacatcggc ggcctgtccg ggaataccct catcgcggcc ggaaaattag
     8641 ggtcggaacc ggaaatttta atcaacatgt acaaagacct gatcgaagac gaaacggata
     8701 tcaaggtgac cgtaaagccc agtttcggga agaccagctt cttatatgaa gctctgaaaa
     8761 acggctccat cgacatgtac cccgaattta cgggaacggt cacgtcgtcc ttgattaagc
     8821 cgcctatgac gggcttgtcg aacaatccgg aaaccgttta tgaagcggct aaagcgggaa
     8881 tcttgaaaca agatgacttg atcctgctgg aaccgatgga ataccaaaat acctatgcca
     8941 ttgccgtgaa acgctcctat gccgagcagt acggcctcaa gacgatttcc gatttgaaaa
     9001 aggtggaaaa cacggccaga gccggtttct ccttagaatt taacgaccgc gaagacggca
     9061 ataagggcct gaaatccctg tacgacctga atctttccgt tgtcaccatg gagccgagcc
     9121 ttcgttatga agccatcgac aaggacaatg tcgacgtcat cgacgtcttc tcgacagacc
     9181 cggaaatcat aactcatgat ttagtcatgc tggaagacga caaaggcctg ttcccgccct
     9241 atcagggagc acctctcctt cgggccgaca ccatcaaaaa gtatcctcaa ttggtaccgg
     9301 tgttgaataa gctggccaac atgattacga cggaagaaat gctcaagatg aattacgaag
     9361 tcgatatcga aggcaagccg gccgccgacg tagcgcgggc gtacttagta tcgaaaggac
     9421 tgctcaagga gtagcagaaa gacacaaaag gccgacagaa ctagtacaaa ctagctccgt
     9481 cggccttact ttatgagagg cttacatttt atcaatcaat ggtgaacgag taaaagatta
     9541 gtcttcccgt tcgttatcca tcggttcgcc tgttttcgtt acttctacgg ctttggcata
     9601 gctttctaac agcagccgat agttggtgaa aatacggccc caaatctgag cccaggcgtc
     9661 ggcttttggt gtatgccagg tgcgctgtaa ttcggctaat acggcaccgg cgtcagttat
     9721 aacgaccccc gcttgggtaa ggcgcatcat ggtagcgata gctgcttctt gtgaatacgt
     9781 accggaagcg tcgggcactg caaatacacg gtatccttcg tttaccgcgg aaagagccgg
     9841 taaggccatg caaacgctgg ttaccgtacc ggcaatgatt aaggtctttt tgccggttgc
     9901 ttttacggcg tctacgaatt ctttgcaatc ccaagaatta acttctccgc ggcgagctac
     9961 atataccgca tggggagcgg cttcatggag ttccggaatc aacggaccgt ttggaccttg
    10021 ggggacggaa gcggtggtaa taacgggcat atttactaac gtcgccgctt cagccagcgc
    10081 aatggctctg tcacgaatgc gcaggaaatc aatatcgcca atacattgga ataaaccgct
    10141 ctggtggtca atgagcagca tggccgcatc attcacgtca atcatggggg cttttccatc
    10201 aaagtttttg taaagttcca tagtaatctc ctttcgtcta aaacaaccat caataagtaa
    10261 gccttacacc ttgattataa taatggctat attcgaaaac aatatacaga aattggaata
    10321 tgtatatagt tagatgatag gttctcaaaa taatatacag aaattgaaat gtgtctagag
    10381 ttggtctcta tttttacaga aaccctattc tccgtgataa aataaaagat aatcattcat
    10441 ctatgatttc aacagtaagg agaaacacat tgactcaaag ccgaaataaa aagacagaaa
    10501 cgaaacgaca cgtatccagc tatactcgaa atcacataaa agaaaaatta ctgcgtatgt
    10561 tgaaaacaaa gcctttccgg gacatccgta ttaccgtcct ttgccaagaa gcacatattg
    10621 ccagaggtac tttctattta tattacaaca cgattgatga cttattagag gagattatta
    10681 acgatagcct ccatctcatc gatcatatga cgccaaatga aaagaagggt tctcttaaag
    10741 aattgcaagc catgttaaac caaacgaatg atgccgaatt accgagggat cggtctctgt
    10801 tccctccctg ccataaatat ttggaacttg aagactatcg ctttctggta aaggatcggg
    10861 atgtagctcc gatgatcgtt gagaaaatct atcagcatga aagtcctaaa attgttccgg
    10921 tactcatgaa agagctgcat atgagccaag atgaggcaga actccttttt caatttatcg
    10981 tcaatggaag cagtgcggtt aaccgaaatt tattggcact aaaagaaaac gagtggtatc
    11041 gcctgcaaaa aagaatcctg caatttattc tacgagggct gaactgtgat aaatggagtt
    11101 aaatcgcgat attatgccgg gcatcctatt cagccgcata gatggcagca cttcgtgtgt
    11161 tgatgaggcc ggcggaaggg gccgttaatt tggcggatac gt
//
LOCUS       DAWDXZ010000004        10912 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_2323_length_10912_cov_7.462375, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000004 DAWDXZ010000000
VERSION     DAWDXZ010000004.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 10912)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10912)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10912
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2323_length_10912_cov_7.462375"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    303..312
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2017..2026
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7858..7867
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2680 a         2903 c         2632 g         2667 t
ORIGIN      
        1 gacaagatga aagaatacac cccttttaaa tccggtaaag tacgtgaagt ctatgacgca
       61 ggtgacagca ttatcatggt tgcaacggac cgcatttccg ctttcgacca tatgctgaaa
      121 aacaagatca ccaaaaaggg cgccatcctg acgcagatgt ccaaattttg gttcgatatg
      181 acgtccgaca tcatcccgaa ccacatgatc tctgtcgaca acgctgacat gccggaattc
      241 ttccagcagg aacagttcgt cggcaacagc atgaaatgcc agaaactgac gatgatccct
      301 atnnnnnnnn nnatgcatcg tccgcggtta tatcaccggc agcggctggg aaagctacca
      361 gcagaacggt acggtttgcg gcattaaatt accggcaggc ctcacagaat cagaaaaact
      421 cccggaaccg atcttcacgc cgagcaccaa ggctgaactc ggtgaccacg atgaaaacgt
      481 ctccctcgaa gaaggcgccg cttacatcga caaagtattc ccgggaaagg gcatggaata
      541 cgctgaaaag attcgcgact acaccattgc cctctataaa aaatgtgccg aatacgccct
      601 ctccaaaggc atcatcatcg ccgatacgaa attcgaattc ggtctcgatg aaaatggcac
      661 catcgtctta ggcgacgaaa tgcttacgcc ggacagctcc cgcttctggc ccctcgaagg
      721 ctataaagca gggcaaagcc agccgtcgta tgacaaacag ttcgtccgtg actggctcaa
      781 ggccaacccg gacagcgact acctcctgcc tcaggacgtc atcgacaaga cgattgaaaa
      841 atacgaagaa gcctatgaaa tgctgacagg caaaaaaatc taatcccatc caggcatcaa
      901 aaaaaggact gcacccatcg ggtgacagtc ctttttcgtt atctaccaga cttttacctg
      961 aatccactga tcgttataat acttatcttc ccgttcccct aagtagcggt acgtttcctg
     1021 tccggcatag gcttcttttg gcgggaagac gacgggatta tccttataat cttcatcttc
     1081 ttccatctcc ggaacggcga tattcggcgt cgaataggtc aggtattcaa agttcttcag
     1141 agcaatgtcg ggacggctca tgaagtcgat gaatttatgg gcattttcga cgtgggccgc
     1201 atttttagga atgacccagg aatcaatcca aaggttggtt ccttccttag gaatgacgta
     1261 ttccaaattc cgattggcca agatcatccg cagggcttcg ccggaaaaga cgacacccat
     1321 ggccgcttcg ccggagacga gtttatcttt cacatcgtcg atgccgtaag cctgaaccaa
     1381 aggcttctgc gcaatgagca ggtctctggc ctgtgcaatt tgggccggat ccgtcgtatt
     1441 tagggaatag ccgaggagtt tcaaggcgac catgaaggaa tcgcggggcg aatcctgcat
     1501 gatgatttcg ttcttatact tttcattcca caaaatggac cagctgtcga cagggtcatc
     1561 gaccatggtc ttattataga cgatgccgac ggtgccccaa cagtacggaa cggaatagcg
     1621 gttgcccgga tcaaagaact ccgactgctg ccagaactgc tctccgacat actgccgcgc
     1681 attgggcagc tgcgaatagt cgaggggctg cagcaacccg ttgtgaatca tcttttgaat
     1741 catatagtcc gacgggcaaa tgacgtcata gcgttcggcg cctgccgcca ctaacggata
     1801 catactttca ttggtgtcga attcatccat gatgacgcgg atgcccgttt cttcttcaaa
     1861 ctgtgtaatc gtatcggggt cgacatagtc cccccagtta tagacgtaga cgacgttgtc
     1921 tccgtcttcc tccccttcca ggaacccgca gcctgataag gccaagacac aaaagagtcc
     1981 catgaggagc agtcgaatat atttcatgac aggcctnnnn nnnnnntcag tcggcagcgt
     2041 atcgatgccg gcgcccttcg taaaatgggt gatgatgaaa tcatcggcag acatggtaaa
     2101 ggccagcatg aatccggcta agagactggg acgcagctcc ggcaggacga cgcggcgaaa
     2161 ggcaacaaaa ggcgtggctc caagatctcg tgccgcatcg tagcagctgc ggtcgaggga
     2221 caggaactga ggcatgatgc acaagatgac ataaggcagg ttaaagacga catgagccat
     2281 aaggatggaa ccataaccaa ggcgcagacc catgccgaga aagagcagca tcagtgaaat
     2341 cccggtaacg atatcggcat ttaaaagggg cagactggct accccgagga ggaccgagcg
     2401 ctgcttgcgt cccataccgc gcaaagccat gcaggtaatg aggcccagga tggtcgccag
     2461 agccgccgac gtcaggccga tggtaatcgt attctgcaag gcttcgagga ttccttcatt
     2521 ttcaaagagt tcaccgtacc actgcaaagt aaaccccgtc cagtgaccgc gatagcggct
     2581 ggcgttgaag gactgggcaa tgaggacgcc gatgggcgca tagagaaaga gaacgactaa
     2641 ggccacatag ccttttttcc atttttccat catttcttca cctgcccttc actgcggtca
     2701 aagtaagccg tcatcaccat attgacgatg atgaacagca tcagcaccag ggataagccg
     2761 ctccctaagt gccagtcgta agccattgta aattcctgtt cgacaatgtt gccgatgagg
     2821 aggaccttat tgccccctaa aaggccgctg atgaagaacg tcgtcagcga aggaataaag
     2881 accatggtaa tgccgctgat ggcaccgggc agggacaagg gcaggatgac cttggtaaag
     2941 gtctgccagg aactggcccc aaggtcgcgg gcagcggcaa tgacgttgcg gttgattttg
     3001 ctcaaagata cgtagagggg caaaatcata taaggtaaaa agttatagat catgccgatg
     3061 acaatcgctg ccggcgtgtt gatgagcacg acgtccggca gggacaaaag gcgcaagaac
     3121 tgattgagga taccgttctt ttcaagaatc gtctgccaag ccatggtcgt cagcatggaa
     3181 ttcatccaca taggaaggac gaacaacaga aataagaggg tactcttact ccgcttttct
     3241 tcagtcaaaa tgaggcatag cggataggcc acgagaagac agacaaccgt actgactaag
     3301 gccaggagca aggacagttc cagggatttt aaatattccc agtgagcaat gaggctgata
     3361 ttcgctaacg taaaagtccc gctgctgtcg gtcaggccgt accaggcgat ggccacgagg
     3421 ggacagaaaa taaacaggat tgcccaaatg aggaagggca acgtaaacag attgcggatc
     3481 gctgccgagt taatcatttt ccatcgccct ttcgtcttcg gattccggtt tattcatgat
     3541 ttggatatta aaaggatcga cttccaagct tacttcgtcg cccggttcat aattcttcgt
     3601 cgatttgacc agccattcga tattccgggc ctgaatagta atgttatagt tcatgccttc
     3661 aaagatgact ttagtgacaa cgccgttgaa gccgccttcc cgggccggcc gcacttccat
     3721 gtcttccgga cgaatctgaa cgtcgacggg acgattcgta ccgaagccga cgtcaacgca
     3781 ttcaaaatca tagtcggcga tacggaccaa tttatcgtgg accatgaggc cgtcgacgat
     3841 gttgctgtcg ccgatgaagt cggcaacgaa agcattttcc ggttcattgt agatgctttc
     3901 cggcgtgccg atctgctgga tatagccttg gttcatgacg acgacgatgt cagacatgct
     3961 gagtgcttcc tcctgatcgt gggtaacgaa gacgaaggta atgcccaact cttctttaat
     4021 cttgatgagc tcgagctgca tactgcggcg cagcttccgg tccaaggcac tgagcggttc
     4081 atcaagaagc aatacccgag gctcattgac gatggcccga gcaatggcta cgcgctgctg
     4141 ctggccgccg gagaggtcga cgatgttggc gtgttcatag ccgtcgaggt taacgagttt
     4201 caaggcatag cggatcttat cgcggatgta gtcgcggggc ttgccgtgaa tcttgggccc
     4261 gaaagcgatg ttctcggcga catccatgtg cgaaaaaagg gaataattct ggaacaccgt
     4321 attgacctga cgccgattgg gcggcaactg ggtaatatcc ttcccatcaa agaggattcg
     4381 ccctttatcg ggtgtttcaa aaccggcaag gagccgcaaa gtcgtcgtct tgccgcagcc
     4441 gctcggtcct aaaagggtga caaatgaatt ttctgaaatg gtaagattca actcgtctaa
     4501 taccagatga ccctcatagg atttggatac attgtccaaa taaatcaatt cttttgccat
     4561 tctcacttct cctttttatc caacaggact ctaaaaaaag ggatttcgca caagctgtcc
     4621 cttatgcgag gtcccttctt tctatgatgg aatgtggttc ctgccccgta tgcgaagtgc
     4681 cttactaaaa ggcaccgtct ttgcaggaag cctgatttag gcttcctgat acattaggta
     4741 tactttattt tattatagca tataccgttg tattttttca aagacaggat acactcttcc
     4801 gtttcaggac agttcaacgg ggacaaaatc aaaggcctcg acgtcgaata atcccgtatc
     4861 gagtaattta agttttggaa tgaccggcag gctcatgaag cacagcgtca tgatgacatc
     4921 gacatcggta ctgacaccta agagactgcg agccttgtca taaaggtcat cgagagcggc
     4981 gtcgacttcg gcagccggct tatcgctcat caggccggca ataggcagcg gaatggcagc
     5041 caataccttt ccgtcaagga tggcggccat accgcccttc tgtttttcca agacttcgac
     5101 agcagcagcc atatccttat tgctggcgcc ggcaacgatg atattatgag agtcgtgggc
     5161 aatggaaacg gcaacagctc ccttctgaag cccgtagccg cggagcagac cgagtccgac
     5221 gttacccgta ccgtgatggc gttcgacgac ggcgactttg atgatatcaa ccttttcatc
     5281 aaagacgaag tcgccctttt catcgcgccg gatctgagct tcgccctttc cggtaacgac
     5341 gccgccgggc atgatatcga tggtccgcac atggtcggct ctcgtaaagg gaggcagata
     5401 atggccgttt tcagcgactt tctgcccttc gataaaggtc gcccggacct tgaagtcctg
     5461 taaatcttca aagaggacga tgtcagctcg cttgcccggt gccaaggcgc cgcggtcatc
     5521 gaaatgatag cattcagcag catttaaggt gcccatggta atggctgctt ccggcgagag
     5581 accggcggca acgcataagc gcaggtgttc gttaaggtgc cctttttcaa aaattgtctt
     5641 aggatgaagg tcatcggagc acaagaggaa ccgccgcaag ctttccggcg tcacggcctt
     5701 gaccaatttc ggcaggtcgt ggcaggccga gccgttgcgc aagagaacgt acatcccggc
     5761 ctggacccgt tcttcggcat cacttttgct gaagcattca tggtccgtca aaatacgggc
     5821 cgaaatgtag gcctggagat ccttgccatg gacgccggga ctgtgaccgt caatgggttt
     5881 tcccgtacca taggccgtaa tgagcttatc gaggacggca tcgtgtcctt caaggatacc
     5941 cgggcagttc atgaattcac cgacaccgag gatgcggtca tctgccagag gcgcttccat
     6001 gtctttcgct tcaagggtgg caccgctgtg gtcaaaaggc gtagccggca cacaagacgg
     6061 caccatgaaa tggatatcga gggctgtttt ttcagcggcc ttcatcatgt attccaagcc
     6121 ctttaggcca cagacgttga cgatttcatg gggatcggcc acgatggtcg tcgttccgaa
     6181 aggtacgacc aggcggccga attcttcagg actgacataa caggactcga tatggatatg
     6241 gctttcaatg aggcccggtg ccgcataggc acctttcgca tcgatgacgt tcttcccttc
     6301 ataagggccg ccgacgccgg caatgacgcc gtcagaaacg acgatatctc cctttcgagt
     6361 ggtctgtgta tatacatcta caatatggca atctttaatg acaaggtctc ccggttcaag
     6421 gccccgggca acggcaatac gccgttttaa ctggttccgc gtcactgtca taatgtaccc
     6481 ccttatttcg ttaatatcat atattttatt gttctgactc attgtaccat attctccctt
     6541 tttttgacaa aagtattgcc caaatatgct aaagccccta taatgataat attccataat
     6601 atctacttgt cattattcat tatattcatt tatggcagta ttaaaaaagg agtgtgaaca
     6661 agttatggaa aaccaaccgt cttttctcga ccgtattttt catctccgtg aaaacggcac
     6721 gacggtacgc accgaaatcc tagccggggt tacgacgttt atgactatgg cttacattct
     6781 ggccgtcaac ccgacaatca tgagcgccgc cggtatggac aagggcgccg tcctgacggc
     6841 aacggccctg gcttccctca tcgggacctt gtgcatggcc gtttttgcca actatccctt
     6901 cgctttggca ccgggcatgg ggctcaacgc cttctttgcc tttacggtcg tactgcagat
     6961 gggctacacc tgggaaatgg ccttagcagc cgtcttcgtt gaagggatca ttttcatcgt
     7021 cttgtccctg accaatgtcc gcgaagccat tttcaacgcc attcctatga ccctgaaaaa
     7081 ggctgtttcc gccggtatcg gcctcttcat tgcccttatc ggcctcttga atgcccaaat
     7141 catcgtcgcc aatccggcta cgaaaatcgc cctcttctcc tttaagcagt ctgccgctac
     7201 gggcaccttc aataccgtgg gcatcacggt tctcctggcc atgatcggta tcatgttcac
     7261 cggggtcttg atggtcaaaa aagtccgcgg caacatcctt tggggcatcc tcttcacgtg
     7321 gatcctggcc atcatctgtg aacttaccgg cctctacgtc cccaatccgg aattacaaat
     7381 gttcagtgtc atcccggatc tctccggcgg tgccgctgcc tttaccccgg ccagcctgag
     7441 cccgattttc ggacaactcg acttcagccg cgtcttcagc ctggatttct tagtcgtcat
     7501 gttcgccttc ctcttcgtcg acatcttcga caccttagga accctcatcg gcgtatcgtc
     7561 caaggctaac atgctcgacg aaaacggccg tctgccccgc atcaaaggcg ccctcttagc
     7621 cgacgccgtt gcaacgacct ttggtgccgt cctcggtacc agcacgacga cgaccttcgt
     7681 cgaaagtgct accggcgtca gtgaaggcgg ccgcaccggt ttaacagccg tctgcgtggc
     7741 catcctcttc gccttatccc tcttcttatc acccttcttc atggccattc cggcctttgc
     7801 tacggcaccg gccttggtca tcgtcggctt cctcatgctg actgccgtcg tcggcatnnn
     7861 nnnnnnnaca tcacgatcat tgccatgcct ttctgctaca gcatttcaga aggtatctcc
     7921 ttcggcatta tctcgtatgt cttcatcaac gtcttgacca acaaacggga aaaaatttcc
     7981 ctcctcatgt acgtcctagc cgtcatcttc gtcttaaaat atatcttctt ataaggtgat
     8041 agcaaaaagg ctgtaccccg tgagaaacaa aatcacggcg tacagcctct ttttttgtgc
     8101 ccaattttaa ggcggccaaa cacagcctca atccacgcac ccgtttgggt gcgacgaaga
     8161 accggtggtg gaaacgaaat gaaaagagaa gtttcaatcc acgcacccgt ttgggtgcga
     8221 catgtcgctc gtcagttagt cattgacggc gacttagttt caatccacgc acccatttgg
     8281 gtgcgacatg gggttgtagt atccctatcg cttatccact cagtttcaat ccacgcaccc
     8341 gtttgggtgc gacagcgaaa tttttatttt tccgcttctt tcttttacat cgatgtatct
     8401 aagttcccac taccgagtct tattcggcag tgattaggga caaggtttac attttttcta
     8461 ttttatcccc tcctttcacg gggaattatt atggtcactt ccccttcgca tttatcatcg
     8521 tctttatctg ctcgggcttt gttaagacta ttatagcaca cgtgattttt ctttctgtaa
     8581 gaaaaatcag tttttcctca tctttcacgc tgtactaaag aactccctat taggaatgtc
     8641 tctgtcctct ccgaactttc tcttgatcct ccttacattc tctcctcgaa tgacttataa
     8701 tagaaacatt ccagcatcat gaggagcttt accattatga aaatcacgca gatactttat
     8761 acaaattcat tctcaggcct atcctgccgc ggcgaagagc gtttcctgcc ttacggcgag
     8821 ggtatttgtc ttgccggccg tcctgtgccg acggcgcggt tatggaaaaa gttaaaagcc
     8881 aatccggtcg taacctttcc cgatcagacg ggagatatga ccggtcaatg tcacctaacc
     8941 gaggaaagtt tcacgccgcg caaggctgaa tataccttac accgcacgga caatatcgag
     9001 acaaagaaga ttgccacggt aattatcgag caattttctc ggcccacggc catcaagggc
     9061 cacaaggtcc gcggccgcat tgccgtcacc gatacgacag cgccatcgga tacggctgaa
     9121 gccttgaaac tgaccggcgt cctcgatacg gccggacaga ttgccttggg actgggattg
     9181 attcgcctcg tctataacga taccccgccc gttttcgacg acgaggctac aagcctgcgc
     9241 gacgaattga ctcagaccaa ggccgcattt caagcgttgc aggaaaatta tgacggctat
     9301 gtgaagacgc tggaacagtc cctccaagaa tgggaagccc gctgtcccca agaggattta
     9361 gagcaaggcg cctttcccct tcccgacgac tacacggcac gccttgaaaa agcgaccgat
     9421 acctttacgg tctgcgtcgc cggcggcaat gacttgtggc acgaaaaaat caggactcgc
     9481 tttcccgact ggacgatttt ggagaacaag aatttcgacg gccaaaaatt acagggcgcc
     9541 gacattctga tcatcaacac caatcacacc ggtcacgccc tagtccgcaa agcctgccag
     9601 caagccgctt ctcaaggcac gatggtctgc tataccagcc gatacaatct caatgccgtc
     9661 gctgccgaca ttctctacgc cttgggagaa tagtctgcca ctgtcaaatt tccataaaac
     9721 gcagcattcc agaacaaaaa aagtccttcc ggagacaggt ttcaacctgc ctgtgaagga
     9781 ctttttgctg tcatattttg attacactaa gtgtttcggc gccgacgaga tggccccttc
     9841 gaggctttct ttttcggcga agtagatgcg ccgcgccatt tcatcgacct tatgggtcaa
     9901 ccacatatag gccgtcgtcc cttcacggta atcggcttcg gcatacatgt cgatgcggcc
     9961 gtcttcgcgc agctgattga cttgttggaa ggccgtgtca tttcccttgg gatagatggc
    10021 aaaggtcgcc ccgccgaatt tattgatgac cgaaaaggcc ggcacatcac tgggcccgtc
    10081 ggcaatgtaa atcatattcg gaaaggccac gcggcgatag gcgtcggaca tcttggcgtt
    10141 gacatcgata tcgggccgtt gaggaacgcc cttattgatt tcgaacaagg cacgcgtctt
    10201 cgtcgtgttg tcgatggtgt agccgatttc actgatgatt ttctgttgat tttcgtcaac
    10261 gtcctcaatg aattcacagc cccaaatagc ttcgacgtaa ggcatgacag ccgacccctt
    10321 gataatggcc aacatccccg tgctgacgat atagtgttcg accttgatat tgtattcctg
    10381 atagcgagga ttattttcta cgacggcttt gagattcttg aaaatatcgg gaacaccggg
    10441 ataatacttc tgtttttgac caaagccccg gagtttttca ttgttcagtc ccttaaaagt
    10501 cccttttcgt gtctccttaa tgaactgatt caaataaatc gtatcgccat tgacccggat
    10561 accttctttg gcatactgag cgggcaagtc attgacttcc ttccaaaact cggtcttatc
    10621 gatatggtat tcttcaaaaa tcggatcctg catattcccg tcaataaggg tcttatcaaa
    10681 atcccaaatg acggcgatga tgttctgcat aggcttcacc tcgctgcttc actcgaagaa
    10741 aaaagagctc tatattcgct taaaaggaaa aggtatcttt cagtcccaac cattgctggt
    10801 ctttatcact catcgtaagg ggcgtgccat cggccagggt atagccggca gcacggcagc
    10861 ttccgtcata atttcctttc aggccgggcc aaaggatgcc gatttccgga tc
//
LOCUS       DAWDXZ010000005        10812 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_2341_length_10812_cov_8.917635, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000005 DAWDXZ010000000
VERSION     DAWDXZ010000005.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 10812)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10812)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10812
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2341_length_10812_cov_8.917635"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6351..6360
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8768..8777
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2677 a         2793 c         2578 g         2744 t
ORIGIN      
        1 cgacgtcgtt gccgcggctg acagcgatat aataagccag cagctgcaag ggaacgatag
       61 ccaggatgga aacgacgaag tcatccgaag caggaacctt gatcaaggta tcgacagtat
      121 ggacgacttc tgtgtcgtcg tcctgagcga tgccgacaac aaaggcatca cgggctttga
      181 cttccttaat attgctgatc atcttgcctt tgacttcgac ctgtgtcgcc aaggcaatga
      241 cgggaacacc ggcttcgatg agcgacagtg taccgtgttt caattcaccg ccggcatagg
      301 attcagcatg gatataagaa atttctttta atttcaaagc accttccatg gccaaaccgt
      361 agtccatgga acggccgatg aagaagacgt cttccttatc tttgaaggca ctgcacaaac
      421 ctttgatcgt atcgacctga tcaaagatct gctggcactg atccggaagt ttttccaggt
      481 gagctaagat ttttgcttcg cggtcagccg gcaaattgcc gttcaatcga cccatgtaaa
      541 gggccaaaag gagcaaggtt accagctgtg tcgtgtaagc tttcgtcgaa gcgacagcga
      601 tttcagggcc tgccagggtg tacgcaactt ggtcggcttc acgagaaatg gacgatccga
      661 cgacattggt aatcgccaac gaacgggcac cgcggcgttt cgcttcttta agagctgcca
      721 tggtgtcgat ggtttcgccg gactggctga cgacgatgca cagcgtcttg ctgtcagtta
      781 agggatcgcg atagcgatat tccgatgcga tatcgacttc aaccggaatg cgggccaatt
      841 tttcgataaa atacttggcc aagagccctg catggtatgc cgtaccgcag gccgtgatca
      901 aaatcttgtc gatagatttt acatcttccg gcgtccagtt caattcgtca aaggaaacat
      961 gctttccgcc atctacaatg cggcggccca tcgtatcctt gatagccttc ggctgttcat
     1021 agatttcttt gagcatgaaa tgttcaaagc cgcctttttc agctgcttca gcatcccagt
     1081 ttacttcaaa gactttcttt gaaatcggat tgccttcaag atcggtcacc caaaccttgt
     1141 ccttcatgac gatggcaatt tcgccgtcat tcatgatgta ggtcttacgg gtgtagctga
     1201 ggatggccgg aatatccgag gcaatatagt tttccccgtc tcccaagccg acgatcaacg
     1261 gattgtcttt tttggtgcaa atgattttat ccggttcaaa tttgcacatg aaaaccaagg
     1321 aataggagcc gcgcaggagc gtcagaacct tgcggaccgt acgggtaaaa tcaccgtcgt
     1381 aaaggtcttt gcaaaggtga gcaatgactt ccgtatccgt ttccgaacgg aagtggtacc
     1441 cttttttaag caggccttcc ttcaattcca tgtagttttc aatgatcccg ttgtgaacga
     1501 tgacgaaatc gccattttca tccgaatggg gatgggcatt gatgtccgac ggaccgccgt
     1561 gcgttgccca acgggtgtga ccgatgccgg acgtcccgac gacgggatgt ttcttgactt
     1621 cttctgccaa gttggccagc cgacccgctt ttttgcgaat agcaatagca ccgtcatgat
     1681 agacggcaat gcctgccgag tcataaccgc ggtactccag cttgcccaga ccatccatta
     1741 aaaagtctgc cgcttccctg tctccgatgt aacctacaat accacacata gttatatcct
     1801 cctgatataa agttttttcc ctcttatgat gaagttgtcg acaatgttac cactgtaatt
     1861 ttccatcata tgacgactga aggtgtgccg agagacatcc gctgacaatt cgatacttct
     1921 ctacctcgtc tacctgaatc gcttcaggta ctagcgctat ccattataac gttaaggatt
     1981 tccaaacgcc tgcatataaa aacaggaatg gagctcaatg aactccattc ccacacttta
     2041 atatatttgc aagattttgt caatacctac ttagaaggcc ccatttcatg ttcgacgaca
     2101 acggcgatat catcgacgat tcgttccaat tgtaccaggt ccggtccttc tgccatgaca
     2161 cgaatcaagg gttctgtccc tgacggacgg acaagaatgc ggccttcatc gccgagttct
     2221 tcttcgcccg aagaaatggc ggccatgata aggttgttgt cttcccagcc tgacttagtc
     2281 tgaacgcgaa cgtttttcaa aatctgagga tagcgagtca tgagagctgc caattcagat
     2341 aagggtttat tctgctgttt catgatgctc agcagctgca cggccgtaag caggccgtca
     2401 ccggtcgtgt tgtaatcgag gaaaatgata tggcccgact gttccccacc gagagaatat
     2461 ccgtcttttc tcattttttc caagacatag cggtcaccga cagccgtgat ttccgtcttg
     2521 cagcccattt ccttcagggc tttatggaaa ccgatattac tcatgaccgt accgacgacg
     2581 gtatcctgtt tgagtcggcc ttcggccttc aatttcatgg cacaaaggag catgatctga
     2641 tcgccgtcca tatcctggcc cttttcgtcg acagccaggc agcggtcggc gtccccgtca
     2701 ttagcaatgc cgatatcggc accgtatttc aagaccgttt ctttcagact gtcgaggttg
     2761 gtagaaccac agttttcatt gatgttgatg ccgttcggtt tattatggat ggcaatgact
     2821 tcggccccca gccgttcgag gatttccggc cctaagaggg aagccgaacc gttggccccg
     2881 tcatgaacga ctttaagacc ggaaaggtcg atatccgtcg atttgcaaat aaaatccgca
     2941 tattcctgct gcaagtcggt ccaagcagaa atcgtaccga tggcagcccc tgtcggacga
     3001 ctctggaggc cggcccgttc atccttcaac atatactctt cgattttatc ttccatttca
     3061 tcgggcaatt tatagccgaa gccatcgaaa aattttatcc cgttgtattc aaacggattg
     3121 tgagacgccg aaatcataac accggcatcc atatgatgcg ttcttgtcag gtaggcaatg
     3181 gccggagtcg gaataacgcc tgccatatag cagtcaccac cggccgatgc gatgccggct
     3241 gccaagatat cggccagcat ggtgccggaa cggcgggtat cccttccgat aaggaaggtc
     3301 gggtgttctt tattctgccc aaagatatag gcagcggctc tgcccaaatg atatgccaat
     3361 tccggggtca acgtgtcgtt agcaacgcct cgcactccat ctgttccaaa caatctagcc
     3421 atttacttta cctccaaagg ttgatattta cgaatgattt cgacagccgt ttccaagccg
     3481 tccaaaaagg cgctcataat gccgcctgca taaccggctc cttcaccgac cggatagagt
     3541 ccggctgtat cgagagccat acggtcgtca ccgcgaacga tacgaacggg ggcactggtc
     3601 ctcgtttcga cgccggtcat gactacgtcg tcatcgtcaa aaccgtgaag gcgccggcca
     3661 aaatacggca gtgcttcggc cagtgtcgct gtgacaaagt ccggcaggac gtcgtgcaaa
     3721 tcagcccagg tcacgccggg acgataggta tggacagcgc cctcggacgc cgcaccgggc
     3781 cgttttaaga aagagccgac agtctgagcc ggtgcatggt agttgctccc gccggcctta
     3841 taggccagtt cttcataacg gcgctgaaac tcgatgccgg ccaaaggccc gtcaccgccc
     3901 atatcgtcgg gcgtaacgtt gaccacgacg gcactgttgg caatgccgct gtcccgcttg
     3961 taaagactca tgccgttggt gacgacccgc ccttcttctg acgccgctcc gacgacgacg
     4021 ccgccgggac acatgcagaa ggaatagcac gaccggccgt cgggactgtg gtagaccaag
     4081 gcgtaatcgg ccggttccag accaagttct tcagccgggc agccgtattg gtcgcggtcg
     4141 atgacatcct gcgaatgttc gatgcgcacg ccgacggcaa aaggtttctt ttccaatacg
     4201 acgccccggt catggagcat gtaatacgta tcgcgggcac tgtgccctac gcccatgacg
     4261 acgacgctgg ccgggtaggt atccttccca ttgatcgtta ccgacttgac ccgtcccatt
     4321 tcatcgcggt caatggctgt gacacagctc gaaaagtgga cttctccgcc tttggcttcg
     4381 atcatatggc gcaggttctt gacgactcgc cgcaggacat ccgttcccac atggggatta
     4441 taggcataca ggatttcttc cggagctccg gcatcgacga aggtctgtaa aatctgccgc
     4501 agcatggggt gattgatgcg cgtcgtcagt ttgccgtcag aaaacgttcc ggcaccgccc
     4561 tctccaaact ggacattcga ttccggttta aacgggccgc cgcgccaaaa ggtttccaca
     4621 tctttggccc gggtatctac gtcgcagccc cgttcaaaga ccagtggcgc ataaccgtgt
     4681 tcagccaggg ccaaggcagc tgccagtccc gacggacccg tgccgatgac gataggccgg
     4741 ctggccaatg attcactgcc ggggatgatt tccggcgccg gcgtttctgc gatttggcgc
     4801 acgtccttat tatcacggca gcgcttccac accttctttt catcgacaaa ggatacgtca
     4861 acggtaaaca cgaaatagat tcgcggtttc cgtcgggcat cgatggcccg gcggacaatg
     4921 cagataccgg aaatatcgct gcggctgcac cgaagtttct tagcaatcag cgcttccaac
     4981 ggcttttgca tgggcacagc cgtacgaata ttcaatactc taagcacaaa gtttctcctt
     5041 ttctactcat catttcggat atggaggacg gcttcgatct catcaggata catattgatg
     5101 ccgtctaccg gcgggatgat gaccttccaa gacttttctt ccttcatatt ttcaacatta
     5161 atgtccggca aatcgatgga actgatactg cgcagtgtat cggcagagcc cctgatttga
     5221 atgctcgtcg gctgaattgt caccgtttta ttcgccgtct ggccggttat atttacgttt
     5281 aaggggacgc ttttggacat agcgtcatga ccgatggaaa ccttgacatt actctgccac
     5341 ggcgtcattt caagtccctc gacaggcgat ccgtcttcgc tgtacaagtg aattggcgcc
     5401 atcaaagcaa aatcactgtt gcgctggttg accggcactt cgatgacggc ctggctgacc
     5461 tcttggatca ggcggcgggc accactgacg acaacctttt ccggaacgat tttcagttca
     5521 ctgataaagt ctttttcatc taagttgccg acgatatggg gcgtcagttt aaattctttg
     5581 accgtataga catcgacggt tatcttcgtc gacgtcatgc tttgtttttt cagttccgtc
     5641 ccgctcggta tttcgacgtg aatcggcacg tcgaccgtcc cttcctggac gtcggacaaa
     5701 tcgatgtagg ccttgatatc cgaaggacct acgttgatga tcgtattgcg cggccccgaa
     5761 aggcggacat aaaccgtctt gggtacattc gatgtaatat attgagaatt gaggttttcc
     5821 acggtaatgg gaacggtata gctcccttca ctgagcggat tttgttcgtt gatgatgacg
     5881 aaccacaaga cgatagccag gagaaagcag atgattttaa gctgccaatt tttatcaatc
     5941 ttgttcatac tagggcctcc atttaaagat attcgccagg ccctgaggtg tccgggtcaa
     6001 aaatgtcttg aggacattgc gcagggcggt gccgtcaaga tggcgataaa tatggccgcc
     6061 ataagtataa gaaatggatc ccgtttcttc actgacgacg ataatgacag catcggtcag
     6121 ttcactgatg ccgatagccg cccggtggcg agtccccaat tccgtcgaca aggaccgatc
     6181 ggcggtcagc ggaaggaggc agccggctgc cataatgcga ttttcccgaa tgatgacagc
     6241 tccgtcgtga agaggcgtat taggaataaa aatattgccc aagagctctt gtgaaacgag
     6301 cccttcaatg gcaaccccgg tgtcaatata gtcattaaga ccgacttcgc nnnnnnnnnn
     6361 acttcgcgtt caaatacgat aagagcccca gtgtgcgttt ttgataagga ctcgctgata
     6421 gctacgattt cgtcgatgag ccgttccagt tcttccatac tgatgacctg gccggaccgg
     6481 aataaacggc cccgcccgat ctgttccaaa gcccggcgca attcgggctg gaatacgacg
     6541 ggaagggcga caagaatgac ggtcatcccc tgctgcagca tccagtttat gacgtgcagg
     6601 tcgaggactc tactgactaa ggtaacgacg cccaggacca agaggccctt aataagcgcg
     6661 acggcccgcg tattgcggat caggatgaat aacttataga gcacaaaggc gacgagtaaa
     6721 atatccaata tgtccagcaa tcgaaaactg ctgataaaac cttgaaaagg taagtacata
     6781 gggcttcact ccaggctata tataagacaa taaaaataaa ataggtactt ttattttaca
     6841 atattttaag cataaaaaaa agggctgtcc gtaaacgccc tcttgacagc caaatctgag
     6901 ccgtccttat cggggacggc ccttcatgag ctgaatctta tactctattc ttcaccggga
     6961 acagccactg cctgttcctt ggtcatgcct tgatgtacca ggcggaattc ttcctcctgc
     7021 ttggctaaca tttcaaaatc aatggacttc aatctggccg ccaaatcacg ctgaatatcg
     7081 gcaaaggcag cataaatgga acacatattg ctgcagccgc gactgcacga gtccatgtca
     7141 ttgaggcagc gctgcaattc cgtctgccct tcgacggctt cgatgacatc aaataacgtg
     7201 atttccttgg gaagtcgctg caaggcgaat ccgccttcca caccgcgata ggatttcatg
     7261 atgccggcct tagtaaggct gcgcatgatt ttcagcaaaa aacgttctgg tatattctgt
     7321 ttttcagcca gcgctgcgcc ggtaattttc gttccttccg ggagccctga cagataaagg
     7381 accatgcgga atgcatagtc cgtagcttga ttcaatctca taatgctacc cccttacatc
     7441 tttcatcctt attatacaat aaaagacaaa aaaagtatag ttttggtttt catattcatc
     7501 attcccgcag catatttcga ggatcaaaat accggcaccc acgtcacgct tcaccttaga
     7561 ggataaaaaa aactctctct gagacaggac aagattttat aatttcccct gcctgccgaa
     7621 gagagagctt tccatatact atcgcatcct ttcggccagc cttcgccggt ctctcaaagg
     7681 agctgaatga ttttaaagat aattgctgac atggccgcac tgataggaat ggtgacgacc
     7741 caggcgatga gcatactgcg tgctaccccc cagtgaacgg cacgaacgcg ttcggccgag
     7801 ccaatcccca tgatggaccc ggaaacgaca tgagtcgtac tgaccggaag atggagaaag
     7861 gttgccgtaa agatgacaat cgacgaatta aggtcggcag caaagccgtt gatcgattcc
     7921 attttaaaaa tcttggttcc catcgtcgaa atgatgcgcc agccgccggc tgacgtaccg
     7981 agagccatgg acgtcgcaca ggcaatcttg acccataccg gaatttccat ggtatcgata
     8041 taaccgccgg acaaaagggc caaggcgatg atccccatgg ctttctgggc gtcgttcgag
     8101 ccatgagaaa aggccattag acttgccgac aggagctgca tcttgcggaa ttgacgattc
     8161 aaagccaacg gcgaataacg gcgaacgacc cacaacaggc cgatcatgac aaaatacccc
     8221 gtagccaagg ccacgacagg tgataaaatc aaggataaaa taatttttcc aatgccgccc
     8281 gtatcgaggg ctgccgggcc gacactgata cagacagcgc cgatgacgcc gccgatcagc
     8341 gaatgggacg aactgctcgg tatgcggtaa taccaagtaa ggacattcca gataatcgaa
     8401 ccgatgaggg cggctacaat gacgtccaaa ctgatttcgg acggattcaa aactaaatcg
     8461 ccgccaatcg tcttggcgac gccggtactg atcatagcac cggcaaaatt aaggcaggcg
     8521 gccatcataa tggcatggcg aggggaaata gcccgcgtcg atacgctggt agcgatggcg
     8581 tttgccgtat cgtgaaaccc attaatgtaa tcaaacaaaa cggccaacag tacgatggcc
     8641 caaattaacc aatgctcagg catatttcaa tacgaccctt ctgaaggtaa cgacaagatc
     8701 ttcgcactca tcggttacgt cttcaacaga ttgaagaatt tctttccact tgataatttc
     8761 gacaggannn nnnnnnnttt taaacagact ggccatttct gcatgataga gatcatcgca
     8821 ttcttgttcc agtttcaaga ccttcttgct cctagcttcg accttcaaat aatttttctt
     8881 gaggtccttc atatacccga tggatttgcg aatttccttc atagccttcg ccgcaatcgt
     8941 cgccatttcc ttggcaccgt ctgttgcctc tccgacctgg tacatgcaca tcttatccat
     9001 aatttcactg atattatcga ccgtcgcatc gagctggtcg atcatctgct ggatatcctc
     9061 ccgatccatg ggagtaatga atgtcttttg cagccgcttc atggtctcca tgaccaaccc
     9121 gtccgcctga tcggccaagg catcaatttt ttccaacgcc gtttctttcg aaatttcacc
     9181 attaatggcc tggctcaaga gttcagccgc ctcatggcct agtgccgcat gttggtccaa
     9241 cagcgaaaaa aacttttctt ccttgggctt taagtggaat gccataatca tcgatctcct
     9301 tatcgaatag ataaaaacaa gccttctcaa aattaacgag attttttcat gacctttaca
     9361 aggctttaca aaaaattata tcatatggcc tgcctttatt ctacaaaagg agtccttttt
     9421 ctgaccgagg caagtgcaat ctatgcatta caaagatgct gtcatcattc aagcggacaa
     9481 agttaaatta ctttaaattg cccctataac ggctgtgagc tatactatag tcaacgactc
     9541 ccacttatag aagcgggagc ttgtaagtca gagaaacata ggtggtagcg atgctgaaga
     9601 caacatctcc ctaattctgc ctacatcatc aggcagttga cgatgcccgt tttgcaaagg
     9661 agatttactc cgaagcaatc gtttttctct cgtagcgagg gtgattctct ccgaaaatcc
     9721 acatcgtacc aagatgctgg atattcatag ctccgatacg gtcatcgttg gattggtatc
     9781 cgcactggca gcgatacagg tgttggtgag gcttacggtt ttccttatgg atagtagcgc
     9841 acttcgggca acgctgagaa gtgtattttg ccaagacttt caatacttcc gagcgatttt
     9901 cgtgagcctt ataggtcaga aactgctcaa gctgatagaa agtccagctt ctcaggtcgc
     9961 aattttgttt agcggttttg gaaagatttg tttcctcaaa gctgacaccc gttaaatctt
    10021 ccagcacaaa cagcgtatct ttgccgtatt tcgtaacgag tgtcttagaa atccgatgat
    10081 ttacatcgga catccaacgg ttctctcgtc cggaaagggc tttgagtctg cgctttgcag
    10141 attttgtgcc tttggattgg agctgctgtc tgattttgag gaacttatgt cgtttggctg
    10201 caatctttcg cccagaaacg aactccgttt ggccttgctc atcgtaactg accgtcagga
    10261 atcgcaagcc acggtcaatc ccgacaacat ggcggacatt ctccttctga aaatcttcaa
    10321 ccactttggt gacgggaata tgcagatacc acaggccttt gagttcaatt aattttgctg
    10381 tgccaagatt ccatgagccg tccagatatt ccgaaaatgg cttgctttca aaggtgcacc
    10441 gggttcgttt gccaagggtg ttgatagaca ggctttgtcc gttatccacg aagctgtaat
    10501 cacgattgcg aaccaaatcc gcttgcgggc gcttgaagaa gatgggcgtc caaagccatt
    10561 cgagcgtttt cgttatgtat ttccagctgc catcttggtc cttgtatttg tatggctttt
    10621 ggaagagctg ctgcttgacc gtcttatagc gagctatagc cgtcttgatg gacgattgtg
    10681 caagctgcga ctttaagcca aacaacacac gaaggtcgct gtatagttct ttgttgaggc
    10741 tttgataagc caagttgaat tgattgtcga agatgtattg agacacaaaa ttacaagcct
    10801 gacgataatg ct
//
LOCUS       DAWDXZ010000006        10545 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_2385_length_10545_cov_10.318303, whole genome shotgun
            sequence.
ACCESSION   DAWDXZ010000006 DAWDXZ010000000
VERSION     DAWDXZ010000006.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 10545)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10545)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10545
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2385_length_10545_cov_10.318303"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1894..1903
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6470..6479
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7496..7505
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9713..9722
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2674 a         2684 c         2808 g         2339 t
ORIGIN      
        1 cttggaaacg gtgtggaaga agtcagcttt gattctcgtc ctatcgagga aaacctgtct
       61 tcagaagagg cagatgatta ttgggataac atccttgaac agggcgtaga cgatatggaa
      121 gaggctcccg attacagtca gatccatgaa accgtcgttt ccgatgcgga accgacggcg
      181 cacgacgttc agccgaccaa ggctccgtcc ttagccgaag aacgggatgt cctcagtcac
      241 gatatggacg atatcaaacc gattgaagtc gaacagagca gtgtggctgt cgaactcagt
      301 gatgaaggca gcagtgctgt gcctaaagcg gagggcaaga aactgtaccg cctgccgtct
      361 gtttccattc tgaagccagg tccccaacat acggtcggtc tcagtgacga agttcgggaa
      421 aatgctgcta ttttaaaaga aaccctgcag agcttcaaca tcgatgccaa gattttaaat
      481 gccagccagg ggccgtccat tacacgctat gaactggaac cggctgccgg tgtcaaggtc
      541 agtaaaattg tccacttggc cgatgacatt gcccttaaat tggcagcgac ggatatccgt
      601 atcgaagcgc cgattcccgg caaggctgcc gttggcattg aagtgccgaa taagaaactg
      661 accggcgtca acctgcgcga cgtcatcgaa agtgaaacct tccaaaaggc cgcaagaggc
      721 gttcctgtct gcttggggaa agatattgcc ggcaatccca tcgtagccga tttgaccaag
      781 atgccgcacc ttctggtggc agggtctacg ggttccggga agagtgtctg catcaatacc
      841 ttcattgcca gcatcctctt taagcagcgg ccggaagatg taaaattgat cctcatcgat
      901 ccgaaagtcg ttgaattatc aaactacaac ggaattcctc atttgctgac cccggtcgtg
      961 accgatccca agaaggctgc cagtgtcctg cgctgggccg ttcgcgaaat ggatgaccgt
     1021 tacaagcgat ttgccctcac ccatacgagg gatatttctc gttataacga acttcatcct
     1081 gaggaaacga tgccctttat cgtcatcatc atcgacgaat tggctgacct catgatgatg
     1141 gcctccgatg acgtcgaaaa gtcgatcatc cgcctgggcc agaaagcccg tgcctgcggc
     1201 atgcacttag tcctggccac gcagcgtccg tcggtagacg tcctgaccgg ccttattaag
     1261 gccaatgtgc ccagccgtat tgcctttgcc gtatcgagtc aggtcgattc tcggacgatc
     1321 ctggacatgg ccggcgccga taaactcatc ggtaaagggg atatgctctt ttatccgctc
     1381 ggtgcttcca aaccgcttcg cgtccaagga gcctttattt ccgattcgga aatcgacgag
     1441 atggtcgaat ttatcaaggg gcaggacgga cctcattacg acgagtcggt ccaaaaggca
     1501 cagtctgaaa acgccgaaga cagcgtcgat ttttttgaag atgacctcat gcgtcaggcc
     1561 atcgatatgg ttctcgagac cggccaggct tcaacgtcta tgctccagcg ccgcttccgc
     1621 atcggctata cgcgggctgc ccgcatgatc gatatgatgg aagctatgca catcgtcggc
     1681 cccaataacg gcagtaagcc gcgggatatt ttgatgactg ccgacgaagt ccaacagaag
     1741 tatttgggct gaacccctgt accctttgtc gtatctgtta tataataaag gatacgatta
     1801 tagtaaatag ggagagaaag acagtgaagg aatcttcgat cggtgacgta ttacgattag
     1861 cgagagaaca acagggtctg accttacaag acgnnnnnnn nnnccctcaa tataaagcgg
     1921 gaatacttgg aagccttgga agccaatgaa tatgatgcca ttcccggcgc cgtattcgtc
     1981 aaaggcttta tccgaaacta cggcaatttt ttggaactgg acggcattgc cttggtccaa
     2041 gaatataagg cctccgttga agaacggacg ccaagacccg aagtgcggac catcgttcct
     2101 gccaaacatc aaaaaaatag gaaagacagt aagaagaaaa agacgcggca gggaaattgg
     2161 ccggaaatta cgattgtggc cggcatcatc atttttctgt tgctcatcat ttggatttta
     2221 atgtaaataa ggagccatat gcgcgagaat ttcttagtaa ccagtcggga agatatgaaa
     2281 agacgcggct gggatcaact tgattttgtc tacatcaatg gcgacgccta tgtcgatcac
     2341 cccggttttg ccgcagccct tatcggccgc gtcctcgaaa gccggggcta tcgtgtcggc
     2401 ctgatttccc agcctgactg gcacagtgca gacgccttta aagccttagg ccgtccgcgg
     2461 ctggccgctt tgattacggc aggcaacctc gattccatgc tcaatgaaaa gacggcggct
     2521 cgaaagttcc gcagtcgcga cagttattca ccgggtgggg aaacggggca tcggccggaa
     2581 cgggcgacga ttgtctatgc caatcgcatg cgcgaggcct ataaagacgt gcccctcatc
     2641 atcggcggta tcgaggccag cctgcgccgc tttgctcatt atgactactg gtccgataag
     2701 gtgcgccact cgatactcct cgacagcaag gccgatatcc tcagttatgg catgggggaa
     2761 cactccattg tagaaatcgc cgatgccctg gctgaaggta agtccgtcgc tgagatgtac
     2821 gacatccgcg gcatctgcta cgtgaccggg aaaccgccta tttcggacaa gaccgtcgtc
     2881 tgcccgtctt atgaagaggt ccgggccgat aaagcggctt ttgccaaggc ttttaaaatg
     2941 cagtatgaag agcaggatcc tttttacgga aagaccatca tccagccgtc agaaaaccgc
     3001 ttcgtcgtcc agacgccgcc ggcactgccc ctttcgacgg aagagatgga tgccatttac
     3061 gagctgccct ttcagcgtcg ctggcatcct gattacgatg ctgccggtgg ggttccggct
     3121 ttgcaggaag tacagttcag cctgaccagt cagcgcggct gtttcggcca ctgtcatttc
     3181 tgcgccattg ccagccacca gggacgcatc atccagcacc gcagccagga atccctcgtt
     3241 cgcgagaccg aacggatgac gaagcttccg ggctttaaag gctatatcca cgacgtcggc
     3301 ggtccgacgg ccaactttcg tcacgtggct tgtcaaaagc aactgactga cggcgtctgc
     3361 cgcaatcgcc actgtatcgg cagtgaaacc tgtcagaacc tcaatacgtc ccatgacgat
     3421 tatctgcagc tcctgcggaa aattcgtcat gtaaaaggcg tgaagaaggt cttcgttcgc
     3481 tccggccttc gctatgatta cgtattggcc gacggcaaca aagaattcgt taaagaactc
     3541 tgccagtatc acgtcagcgg tcagctgaaa gtcgcgccgg aacacgtcgt caaacacgtg
     3601 accgacctca tgggaaaggc cgatgtcaac gcatttctga cctttaagga ctggttcgat
     3661 gaagccaatc gggaactggg caagaaacag tacttggtcc cctatttcat gtccagtcat
     3721 ccgggctgta ccttaaaaga tgccgtagcc ctggccgaat ttctgcggga catgcatatg
     3781 cagcctgaac aggtccagga ttttatcccg acgccgggca gtttgtcgac ggccatgtat
     3841 tataccggta tcaatccctt gaccgggcaa aaggtctacg tggctcgcag ggcagaagaa
     3901 aagcagatgc agcgggccct tatgcagtac aagaacccgg cgaattatga tattgtctat
     3961 aaggccttat gcctggcggg ccgtcgtgac ctcatcggtt acgggccgaa gtgcctcatt
     4021 gcaccacggc gtcatcaaag tcagtcaggc cgtccgcctg tccgttccgg acaaaggcgg
     4081 aaggatttga atcgaactag aaaattcagg tgaatacaat gaagaaatac gtgccttttg
     4141 tcgtgctcag cgtcttcgtc atcttactgt tgggtctggc gtggcccatc ttgatgaagc
     4201 accagcagga agaagcacaa cgggcttatg aagccggccg tcatctcgtc atcttttcgg
     4261 atttgcccca ggatgtcaat gacggtttgg cccgagaatt ttataagcag aagcatttgc
     4321 gggtgcagat ttattctaag accgacagcg atatgcgcca gtcgctgggg aatatcaacg
     4381 gtcctaagcc ggatatcctc atcgcttcgg aagatgattt gcgcacgcaa aagcaaaatg
     4441 gcattctcca gccgtatgag tcggcctata ccgacaatat cccgtcgtcc atgaaagatg
     4501 tcgatggcat gtggagcggt ttatggtaca atccgatggt ctttgtcgtc agtcgatctt
     4561 attatgaacg gcgaggccag ctcctttcta cttgggatga cctgttgacc gatcctcaga
     4621 tgctgctggc ctttccggac ctggctgcca tggatatggc cggcgaattc ctctgttctt
     4681 tcgtagagat gaagggcctc gatgaaagtg aacgctacct gcgtgccttg caaagtcacg
     4741 tcgcttccta ttcgaaatcg atgtcggtta acgtccgccg cgttgccagc ggggaagccg
     4801 atatcggcgt agccgatgct tcgtctgcca cgcagtatcg aaatgacgga gcgcctattt
     4861 atatcatcta tccccaagac ggtacgtcgt actggctgac ggggatagct gtcactaatt
     4921 ggtgcgaaga cggagaatta gccaatgctt ttgccgattg gatctattct ccggaggcta
     4981 atgccgtctt acagcaaaag cacattttca tgagcgatgc cctgccgcag gctcagcctg
     5041 agctcgatgc caacggacgg ggactcgtct tattcccggt aaaaaaatta tattctgatg
     5101 aaggccgccg cagcttacag tcatggtgga tcaaatcaat tcgcttcgga aaggaaaaat
     5161 aacgatacat ggaaaagatc ggatttgtca gcttaggctg ttctaaaaat ctcatcgata
     5221 cagaagtcat gctgggcatc ctccgcgacc gccacatgga gatcacagag gatctctccc
     5281 agtctgacat tattatcgtc aatacctgta cctttattga aaaggcaaag caggaatcca
     5341 tcgataccat cttgcaggca gcccgctata aaacggaggg caactgcaag attctcatcg
     5401 taacgggctg cctcagccag cagtataaag aagaactgat gaaggaaatg ccggaaatcg
     5461 acgccctctt gggtaccggt tcatgggacc gcatctggga agccatcgat accgtcaaaa
     5521 agggccgtaa agcctgcttc atcgacgatg tcagtcattt gtacgatcag catacgagcc
     5581 gcatgcggac gacgccgacg tatagtgcct atgtaaaaat cggcgaaggc tgcaacaacg
     5641 gctgctcttt ctgcattatt cctcacgttc gggggaaatt gtacagtcgt cccatcgaat
     5701 cgattgtagc tgaagtgcag cagctggctg ccgacggcgt aaaggaaatc aacctcatcg
     5761 cccaggatac gacgagttac ggcgtcgata ttgccggcca ttctctgctg ccggatctcc
     5821 tgcgtaaatt ggttcccatc gaaggcatcc gctggatccg gctgttctat ctctatcccc
     5881 attactttac ggatgaattg atggatctca tcgtgaagga agataaaatc tgtccctatg
     5941 tcgatttgcc gctccaacac attgccgaac cggtcctgcg ccgcatgaac cgccgcgatt
     6001 cggaagacga tgtgcgccgc ctcattaaaa aactgtgcag tcacggccgc aagcttaccc
     6061 tgcgctcgac ctttatcgtc gggttccccg gtgaaacgga agctgaattc caagctctct
     6121 gcgactttgt aaaggaaacg gaatttgatg acgtcggcgt ctttacctat tctcaggaag
     6181 aagggacgcc ggcggccacc atggacggcc agatcccgaa agagataaaa gaagaacgtt
     6241 atcaccagct catggccatc caggccaagg tgtcggaaat ccgcaaccaa gaactggaag
     6301 gttcggtgca tacgatgtta gtcgaaaaag tagaacaaga aaaaggtgtg acccaagccg
     6361 tcggacgcat tgaaatccag gcgcccgatg tcgatggcct gacctatttg gaagatgccg
     6421 agggtgtaca gcccggggac atgatcccgg tccgcattgc ccagggcttn nnnnnnnnnt
     6481 tgcctatgat ctcgtggctg aacggctcga ttaaccgagg tgattgggaa tgatagtaga
     6541 acttgttacg acgggctctg aattattgct gggtgaaatt accaattata attcggccta
     6601 cttatcgaaa cagctcaatg aaatgggata cttcgtcgtc tatcatacga cggtcggcga
     6661 taacccgaag cgtatgaaag acgtcttgaa gacggccctt tcccgggccg atatcgtcat
     6721 tacgacgggc ggtctcgggc cgacacaagg cgacatgacc aagattatcg gtgccgaatt
     6781 gatgaattgc cccctggttt atgacccggc cattgccgat gcggtccaag cctgggtgac
     6841 agcccatcac ggtacggtga cgatgaccga aaaccagaag cgtcaggcca tgattcctga
     6901 aggcgctgat gtctttgaca acgaggccgg cactgcgccg ggcgttgcca tgcagggcgg
     6961 tggtaagaca ttggtccact tgccgggacc gcccagtgaa atgcgatgga tgtatgaaaa
     7021 tcggctgaaa ccatggcttc ttcagacttt tggcagccaa gggtccatcc gctctctcta
     7081 tatcaagatt tatgatatgg gtgaagcttt tattgaagaa cggctgatgg atctcgtcaa
     7141 agaccagtcg aatccgacct tggccatgta cgcccgcccg ggattcgtcg aagtccgcct
     7201 cacggcccgc gccgacagtg aagaacaggc cgaggagctt ctcttaccgc tcgaagaaga
     7261 agtacgccgc cgtctcaatg accaaatcgt cagttttaat gatgagacga tggctgacgt
     7321 cctgggacga gaagtccgtc aggcgggcta tatcgtcagt gcggccgaat cctgtacggg
     7381 aggcttggtc gggtccctta ttaccgatgt ccccggtgct tccgactatt ttcgtggttc
     7441 ggccggaacg tactgcaatg aaatgaaaga gaaactcctc ggtgtctctc atgagnnnnn
     7501 nnnnngcggt acggaagaac agcctgtagg ccttgtctat accggcgtca gcggcccttg
     7561 ggggactttg gtccaccgtg acgtctatca cgggggccgt gctgaaatca agcgccgagc
     7621 tgccgtacgg gccatctatt atgtagtgcg ctatcttgtg aaccataaaa acaaataacc
     7681 gttaggagga atgattttgg acgcgaaaca gacagcactt gaaaatgcaa tgcgcaagat
     7741 tgaaaaagac tttggtaagg gcgctatcat gcgcctcggc gacattgctg acaagatgaa
     7801 cttagaagtt atctcctcgg gttccctggc cgtcgacttg gccgtcggcg tcggcggata
     7861 tccccgggga cgagtcattg aaatctatgg ccccgaatcg tccgggaaga cgaccctggc
     7921 cctgcacgcc attgcgtcag cccagaagaa cggcggcatt gcagccttta tcgatgctga
     7981 acacgccctc gatccggtct atgcccgcca tctcggcgtc gatacgaacg agctgctcat
     8041 ttcccagccg gataacggcg aacaggccct tgaaattacg gaagaactcg tccgtagcgg
     8101 cgccgttgac gtcatcgtcg tcgactccgt agccgccttg gttccgaaag ccgaaatcga
     8161 aggcgaaatg ggcgactccc acgtcggcct ccaggcccgc ctcatgagcc aggctttgcg
     8221 taaattgacg gggatcattt ccaagtcgaa ggccatggtc atcttcatca accagctccg
     8281 tgaaaaagtc ggcgtcatgt tcggcaaccc ggaaacgacg accggcggcc gggctttgaa
     8341 attctatgcc tctatccgca tggaaattcg taaaggggaa gctatcaaat ccggcggcga
     8401 tgtcatcggc aaccgagctc gggtcaaagt cgtcaaaaat aaagtggcac cgccctttaa
     8461 acactgcgaa ttcgatatca tgtacggtca gggcatttcc cgtgaaggga cggtccttga
     8521 cttgggcacg tccatggata ttattgaaaa gagcggcacc tggtactctt acaaaggaga
     8581 acgactggga caaggcaagg aaaatgtcaa gattttcatg aaggaacatc cggaaatcac
     8641 ggacgaagtc gaaaagatca ttcgcgatac cctggcggca gaaccggaaa aattcgacga
     8701 tgtcatggat gaagaaatga tggaatcggc cgctgaagcc gtaccggaaa gcgacgaagc
     8761 ggaataaatg acctatagtg aggcctatga aaaagccctg cagctgctca atgtccgctt
     8821 cttaagtgaa ggcgaactgc gcaccaagct gcggcgacgg gacgtcagag acgacgtcat
     8881 cgacgaagta atggctatgc tgaaagagga acacttcatc gatgatgacc gcctggctcg
     8941 tcaggtctat gcctattatg tacggaaaaa gcagtacggc catctgtata tcgccaattg
     9001 ccttcgacgc cgtcaactga cggtgccgga cgatattgaa cgtcctgatg aatacacctt
     9061 ggcagaaaat cttgccgagc gaaagttttc ccgccggtac gatgaaaaga gaaaggcggc
     9121 gcgatatctc caataccgcg gtttttctcc ctccgtcatc gccgaagtca tcgcttcctt
     9181 tacggttggc gacgagggtt gacaatcggt caaaataggg tcggagacgc caagtccatc
     9241 ttgacattga tatgaaaaaa aattacaatt aaaggagccg gtttgataca ctggtccata
     9301 ttttgcataa ttgtaggttt tttgaacatg aagtaaattt tacatactca taaaaaaagc
     9361 cggacggtaa gttcggcttt tttttgtgaa ggggaggtga atttgttact atgctgacta
     9421 ctactatggc tgctgtcgta gccattgttt gtatcctcat cggcgtcggt gccggttatg
     9481 tctatcgcaa gaaaattgct gaaggcaaaa tcggccaggc tgaagtcgaa gcgcagcgga
     9541 tcattgccac agcccagcag aatgcggaag ctcgcaaaaa agaactggtc cttgaaggca
     9601 aggaagaaat ccataaactg cgaagcgatg ccgagcggga gaacaaggaa cgtcgcacgg
     9661 aattacagcg ctttgaacgg cgtttgatgc aaaaagaaga acatttagac aannnnnnnn
     9721 nnaagtgacg cgcatgcagg aaaaagtcga agccattcac gaacagaaga tggccgaatt
     9781 ggaacggatt tccggcttga cttatgaaga tgctaaagaa ctgcttttga acaacgttca
     9841 aagtgaagta aaacatgaaa cggcacagat gatcaagatg cttgaagaac aggctcatga
     9901 agaggccgaa agcaaggcta gggaaattat ttccgtggct atccaacgct gtgctgccga
     9961 ccatgtcgct gaaacgacgg tgtctgtcgt gtccctgccg aacgatgaaa tgaagggctg
    10021 tatcatcggc cgtgaagggc gaaacatccg ggctattgaa atgctcaccg gtgtcgacct
    10081 catcatcgac gatacgccgg aagccgtcat cttgtcgtgc ttcgatccga tccgtcggga
    10141 aatcgcccgc attgccttag aaaaactgat tgctgacggc cgtattcatc cggcacggat
    10201 tgaagaaatg gtcgaaaagg ctcgtaaaga aatcaaccag aagatccgcg aagccggtga
    10261 acaggcgacg tatgaagccg gggttcacgg actccatccg gaactcatta agattctcgg
    10321 ccgcttgcgc taccgcacca gctatggcca gaatgtactg cgccattcta tcgaagtttc
    10381 ccagctggca ggcattatgg ctgctgaatt gggggtcgat gaaaagctgg ctaagcgagc
    10441 cggcttgatt cacgatatcg ggaaagcctt ggaccacgaa ctggaaggga cacacgtctc
    10501 tatcggtgtc gaaatcgcca agaaatacgg cgaatcgaag ctggt
//
LOCUS       DAWDXZ010000007        10095 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_2483_length_10095_cov_7.798307, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000007 DAWDXZ010000000
VERSION     DAWDXZ010000007.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 10095)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10095)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10095
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2483_length_10095_cov_7.798307"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    360..369
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2547..2604
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4726..4735
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6732..6741
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7247..7256
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9333..9342
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2801 a         2343 c         2279 g         2564 t
ORIGIN      
        1 ccaatatttc ttctaaggcc ttgtagcccc cgtggcactg gatttctaag acatcttcag
       61 ctgtatatga atgaggcccc ttcatgatga gcagcaaaac ttcatcgatt ggccggccgt
      121 catcagacac gatcataccg tgctggatgg aacggtcccg acggttcgcc aaaggaacct
      181 cagaggaact cttaaaaacg gcatcgccga ccgcataggc cttgctgccg ctgatacgaa
      241 tgacgccgat gccgctttca ccgagcgggg ttgcaatggc cgcaatcgta tcggccgtat
      301 catacatcat atatgactcc ttccatagtc taaaaggagt tgtcagcgac aactcctttn
      361 nnnnnnnnnt gcgttatcct tgataataaa tgacgacgtg acggtacgga tctttgcctt
      421 cgctttccgt ccgcacttcc gagtcatttt gcagggctac gtgaatgatt tttcgttcat
      481 agccattcat cggctctaag gtaacggact cgccattttg tttgacccgg ttggccaaac
      541 gatgagccag ctgcttcagc gtttcttcac ggcgatggcg gtagttttca acatcgagca
      601 taataaagta ccgtgcattc tcaccttgat tggtcgtcag gttggtcaaa tattggaggg
      661 catccaaggt ttgaccgtgc ttgcctataa gaatgcctaa gttcttgcca tggagatgaa
      721 gaaggatctt atcggattta atcatctttt caatgactac gtcaatgccc atgttcttaa
      781 ggacttcctg aaggaaagcc ttgcccttgg cagccgcttc ttctgccgaa aaaacagctt
      841 cctccggttc atgtacgacc gcttcagaaa tcccgtcttc ttccacatcg gatggtgaga
      901 cttctggatc ctttgcatct tcagacgttt cagctatttg cggttcttca acgagaactt
      961 ccttttccgg ttcggcctga acgacgtcag acggtaccgg cgtgacatcg gccttttcag
     1021 taatacggac ccgggccggt ttactgccaa atccaaagaa accgcttttc ggttcttcta
     1081 aaatctcgat atcgacttct tctttcattc tgccgaggcg caccagaccg gagcgcacgg
     1141 catcttcgat cgtttttcct gttgcttcga tagtactcat gcttctgctc ctttattctt
     1201 gaggtgagcc ataatcatct gctggcccca ctggaaaagg ttagaaacga cccagtaaat
     1261 gaccaagccg ctcgggaagt tgaggctgat ccaaccgata aacagcggca tgacgacttt
     1321 catgattttc tgctgtttcg cttgagcctc cgaggcagct acctgtgcgc cggacatctg
     1381 gttctgaata aagtacgtcg aaatggccga caggatcggc atgacgtaaa taggatccgg
     1441 ctgaccgaga ctgggaagcc ataaaaagct ttcaaaggca ggatcataag gatactctct
     1501 tagagcatag aatatagaaa taaggaaagg catctgaatg agaatgggaa ggcagcccga
     1561 caaggggtta acgcccattt cccgatacag tttagacatt tcttcctgca tcttcttctg
     1621 atttccctta tatttcttct gtatctcttt aatcttaggc tgcaaaagct gcataccttc
     1681 catggaacgg atctgtttcg ccgtaagggg agacagcacc aatttaataa tcaaggtgag
     1741 caagataatg gcaatgccat agctcggata gcccatgttc tgggtaaaag cataacagta
     1801 attcataaat gtcgtcataa taccactgag gacatccatt attccactga aaaagcccaa
     1861 tgtgctcaac tcctgttcag ttgtaaaaat aaggatttac ggtacgggat catatccacc
     1921 cttatgaaag ggatggcatt ttaaaatgcg ccttattgcc aagtaactcc cttttaacgc
     1981 accgtatttc tgcaatgctt caagagcata ggcagaacaa gtgggataaa accggcagca
     2041 tggccggtga agcggcgata taaataactg gtagccccga atcagggcta tgaaacacca
     2101 tttcatgcat catcacttct ttttcaatac cctggcatgc cggaataact gcaaaataga
     2161 ctgctctgct tctgcatatc cggcatattt cagactgctg cggccaatga ggactaaatc
     2221 gaaaccgtcc ttcagatcaa actgatggag tcggtatact tccttcatca gacggcggca
     2281 gcggttgcgc tcaacggcac aaccgatttt tttccccgtc acaaaaccaa tgcgggtcgg
     2341 ttgttttggc gaccgcggca ggacgtataa aacgacgcgg cgattgacat acgacttccc
     2401 gcggcggtat acgagctggt attcgcgatt tttacacagc cgttgctctt tcgttagttc
     2461 atacatacat aattgtccta gtaatgctag aaaaacagaa aaattccaca tgccggaact
     2521 tttctgttta tctcgtaata attaagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnncttagt cttcattctt tcacgaaaac catgagttct
     2641 ttttctccac aatgtattcg gctgatatgt ctgtttcaag ctagacacct ccttttccat
     2701 cattgactgt aattaattta caccataaca ggttaattat agtaagtgaa tgaaggcctg
     2761 tcaagcctta tttttagtag attattccat ttgagaatac cgtatgcata agtattccag
     2821 caaaaattcc ttcccataac cggggttttg ttgtagaatg tggatagata aatacagtta
     2881 tcaacaatcc atccacattt tgtggataag tggaaagaat cggaaactcg atgtatatcg
     2941 gctttccggc tttttacatt tcatttgttc acgttgtcca caatgaagtt gtacacatat
     3001 gaacgcccat tatacacact taaatttttc gtacagacag gaaggattat tatggatctg
     3061 ctcagtttat gggaaaaaat gttagccaat ttgaaacaag tcttgccaaa agaaattttc
     3121 actaattggt ttgaaggcaa catcattccc tactcgtatt ccgaagaaga aaatctgctc
     3181 atcctcgaca cgatgaccaa ttttcttcgc gattatgtca gcaaaaagta taccgatgtc
     3241 ctggagacaa tcgccgaaga agtcattggc cataaaacca gggttcagct cattaccagc
     3301 caagaaaaaa cgccgaccca cgacaaaacc gaagccgtcg ccgaaacgat tacccttccg
     3361 ccaaaacccg ggacaacctc ccacgtcgga aaaccggccc aggcagtccc taccgaatgg
     3421 gttcaacctg aacttcccat gaacgatacc atgccggcca taaaggatgc cgctccgtta
     3481 tcactgacaa cagaatccag tccttataat aataatttaa ataaagatta tacctttgac
     3541 aacttcatcg tcggcaacag caatcgcgta gctacggccg ttgccaagtc gatcgctgcc
     3601 gctccggctc aaaaatggaa tcctttttat atttacgggg acagcggcct gggcaagacc
     3661 catttaatgc atgccatcgg ccatgaactg ttaaaaaact tcccgaatat gcgtctctta
     3721 tgcattacca gcgaagactt cgtcaatgaa ttcatccaat gcatccagga ccagaatacg
     3781 gaaagcttcc gccgccggta ccgcaacgtc gacgtcctct ttgtcgatga tatccagttc
     3841 ctcggccgcg gtgaaaagga aagctctaag gaagaattct tccatatccg cgtcctctta
     3901 gaagccttgc agaccaagag taatgtcacc ttgccgttgg ttaaacatgc aatcagctat
     3961 atcaccgatg cgcaagatga aaagaagtat attaccatcg atgaaattac gacctatatc
     4021 tgccaacatt ataatatcaa ttataaagaa ttgatgggca aaaaaaagac caaggatatt
     4081 gccctccctc gtcaaattgc catgtatatg tgccgtgaac tgaccgggaa cacttatcct
     4141 catatcggaa cggctttcaa tggtcgtgac catacgacgg ttatgcatgc ctgtaccaag
     4201 atcatggaac gagccaaaga agatcgtcat ttcaaagaac atatcgaaaa gatgatgaat
     4261 gaaatccgcg gtgtggataa ctaggttgat aaaaatatcg atagattgtg gataaggtaa
     4321 aaaaagaaat ccacagtgga aatgtggaca aaaaataact tgttatccac attctatcca
     4381 cacctgtgga tagctgaaat tggctcttat tcatcactta tccacaaatt cacaggccct
     4441 actactaaga ctactatttt ttaattcaca tttaattaat ttactcagcg cagaaaaggg
     4501 gatactccat tgagactgaa actaaaaaaa gaagatctga acaaaggctt acaagctgtc
     4561 caaaaagtag ctcagaacaa aaacaacaat atgtcttctg aaaacggcct tctgattaaa
     4621 gcccttcacg gcattatcga atttcaggcc aatgattatg acatgggaat caagatcacc
     4681 gttcccggaa tcattgaaga aaaaggagaa gcctttattg ccaatnnnnn nnnnnaaggc
     4741 gacaagctga aatttgaatg cctgaccatc aaccccgatg actttaatga agtcgaaatc
     4801 gtcgaaaacg gttatgccag ctttaaaacg acgagtgtcg tcttaaagga tttaatcgat
     4861 aatacgtctt atgcctgtgc cactgacgta gcacgtccga ttttcatggg gaccttcctc
     4921 gatgtccaag gcgatcagat gaccatggta gctacggata cgcaccgcct ggctctgaaa
     4981 aagacgaccc tcgatgaacc ggtgaataat ccgctgcagg ctgttatccc cagccggctc
     5041 ttggcggaaa tttctcgtca gctgccaact gatattccgg agaccgtcga aatcacagcc
     5101 gtccgcaact acctggccgt tcagttcggc aacgtctaca tccgtacgcg ccttatcgaa
     5161 ggggacttcc ccaattaccg ccgcgtcatt ccgacggact ttaaatgtat tgccaaagtc
     5221 aatcgctccg aatttactgg cgctgtggaa cgagcttcca tcgtagccaa agatgctcag
     5281 tataatgtca tcaacttcct catcagcgag ggccagatta aattgatgag tcagcatccc
     5341 gattacggta ccgtcgaaga ctacgtctcc tgcgacatgg acggcgaaga attggatatc
     5401 tccttcaatg gaaaatttat cctcgacatc ttgaaacatt gtcagagcag cgacgtcatc
     5461 ttgaaatcga aagaaaacag cccgatgctc gttcaggata aggatgacga ttcttgtgta
     5521 tttgttgtaa cccctatgag gacaaaataa tggaaacaat taaaattgaa acgccgatga
     5581 tccaattgga ccagtttttg aaatgggcca gtattttaca aagcggcggc gaaatccgct
     5641 ttttattgga tgaagataaa attttagtaa atggaatttt atgcaaagct aagagaaaaa
     5701 aactctatcc gggagatacg gtagaaatta aggatgtcgg tacatatttg attaaaggtg
     5761 aataattatg atgattgagt cggttcgact gcaccggttc cgaaattatg agaatcaaaa
     5821 aatctccttt cctaaggcga cgattctctt atatgggcaa aacggacaag ggaagaccaa
     5881 tcttctggaa tctttatatt taggcggaat cggcaagtct tatcggggca ttgccgattc
     5941 tgatctcatt tcctgggagc acgagaaagc cagcgttatc atcgatttta accgtaacga
     6001 tacgacacag cagataaaag tgatcttgtc caaactcgag aaaaaagaac tgtgggtcaa
     6061 tgaaacaaag gtatcgcaga aagaattgat cggaaccttg aatgaggtcc tgttttcgcc
     6121 cgatgacctt cagctcatca agggcagccc ttctttgcgg cgccgtttta tcgacatgga
     6181 gatttctcag gtcagccggt cttattaccg ggatttactg cagtataacc gggccgtgtc
     6241 gcagcgcaat ctcttattga aaaagatgaa atacaacggc aatcaatcgc ttgatgaatg
     6301 ggatcgtcag attgccggtt ttgcagcgtc catcgtaagc aaacgcttag aagctcttca
     6361 gaaattatcg tttttggcag gggttctcca taaaaggctc agtcggggaa aagaaatgct
     6421 ccatctgacc tatgtccaac cttatgggac tggggagggc cgccatcaga ctgatccgaa
     6481 atggtattat gattgcttac agcagcatct ccctgaagat atccgccgtc aagctacgtc
     6541 ggtcgggcct catcgtgacg atctcttatt ttctgtcggc caaggggatt taaagaaata
     6601 cggttctcag ggacaacagc ggacggctgt cttggcgctg aaattgtcgg agctggaatt
     6661 tatcaaatcg gagaccggcg aataccccgt tctcttattg gatgacgtca tgagtgagct
     6721 cgatcaaagc cnnnnnnnnn nagggagggt ggtttctcat ggagaagcga agtcttaaac
     6781 ttgaaaaagc cggacttgcc attcctgaag tcttggccaa acaccgtctg ctccttccct
     6841 accggatgta ccaggctaag cgggactgga aaaaaatcgt aggcgagcaa attgctaaat
     6901 attcatatat attagatttt aagaataatg ttattgtcat tgccgtcatg aatcccgttt
     6961 acatgaatta tttatttatg tataaaaata aaataataga ggaagttaat tcctatattg
     7021 ggcatcaggc tattgccgat gtccgttttg tcaaaaaggg gaaaaagccg gttcagacgg
     7081 tctatgaaac cttagaagga gaagaaaaag atgtccttcc tgaggggaat atccgccaag
     7141 tcattcttga cgattcgacg gtggccaaca ttcgtcgtga aacggaaaac ctcccggacg
     7201 gcttacggga acgggtcgta cagcttcgtt ttgcccaggc taaacgnnnn nnnnnnggct
     7261 atcgtaccgt atccgaaaca aaagggcgcg gctattttac agtctttcga tctccccgtg
     7321 cgagaggtcg gagtttcgtc agaacggaat tggcgctctt tagtgattac cggggaggca
     7381 gtctattctc tgcccctgac cggggagacc atgcttcgcc gatatcagaa atgtttgcag
     7441 attttgtcgc attgcgaaca ggcgtaaggg cggtacgtat ttaaaaatta ttgattttac
     7501 ggtataataa agtgttagtt gcctagttaa tcattaaaaa ttgcaagaaa caggagttga
     7561 gaatttatat gtcgaaagac aaagcgcgat atggtgccga acagattcaa gtattagaag
     7621 gcctggaagc cgttcgtaaa cggcctggga tgtacatcgg cagtacgtct gaacgcggtc
     7681 tgcaccatct ggtttatgaa gtcgtcgaca atagtatcga cgaagccttg gccgggtatt
     7741 gtacacatat cgaagttacg attcaggaag acaacagtat caccgttgtc gataacggcc
     7801 gtgggattcc ggttgccatg cataagatcg gcaagcctgc tgttgaagtc gtattgactg
     7861 tcctccatgc cggcggtaaa ttcggcggcg acggctatcc catttccggc ggccttcatg
     7921 gcgtcggtat ttctgtcgtc aatgctctga gtgcctggac taaggtcgaa gtaaagcgcg
     7981 acggctatct atgggacatt tcttttgccc gcgggttgac ggagtcgccg ttgaaaaaaa
     8041 tccggccctt ggaagccggg gaagaaacgg gaacccgagt ctcgtttaaa ccggatcatg
     8101 acattttccc cgatacggta tacgatttca ataccctgaa gacccgcctg cgggaactcg
     8161 catttttgaa taaaggctta aacatcacac ttacggataa aagaggcgaa ggcaaacaag
     8221 aagtatactg ctatgaaggc ggtctcgttt cctttgtttc cttcttaaat gaaaaacacc
     8281 aggccgttaa tccgacggtg gtcagcatcg atggtgaaaa aaataaggtt atcgtcgata
     8341 tcgctatgca gtataacgat ggctacagtg aaaatatcct gacctttgtc aatgatatct
     8401 ttaccgaaga agggggcacg cacctcagcg ggttccgttc cggtttgact cgtaccatca
     8461 acgactatgg ccgcagtgcc ggtattttaa aggacaatga agataattta tccggtgaag
     8521 acgtccgcga agggttgacg gctgtcataa gtgtcaaagt ccgggaacct cagtttgaag
     8581 ggcagacgaa gaccaagctc ggaaatggcg aagtcaaagg catcgtcgat aacatcattt
     8641 ctgatggctt gaaagaattt tttgaagaac atccgtcgga agcaaaggcg gtcatcaata
     8701 agaccatttc ggcgtctcgt gcccgccagg cagcccgccg tgcccgcgaa ttgacgcgcc
     8761 ggaaaaatgc tttggaaatc agtagtcttc ccggtaaatt ggccgactgc tcagaaaaaa
     8821 atcctgatca gacggaaatt tacttagtcg aaggtgactc ggcaggcggc agcgccaaac
     8881 agggccgaga tcgaagattc caggctatcc ttccgctccg cgggaaaatc atcaatgtcg
     8941 aaaaagctcg tctcgataag attttggcta acgaagaaat tcgctccatg atcacggctt
     9001 tcggtaccgg tatcggtcag gaattcgaca tcagcaaacg ccgttacgat aaaattatca
     9061 tcatgaccga tgccgatgtc gacggtgccc atatccgcac gctgctcttg accttcttct
     9121 atcggtttat gaagcccctc attacggaag gccacgtctt catcgctcag ccgccgctgt
     9181 atcaggtacg caagggaaaa caggtatggt atttctatac cgatgaatcg atgcagagca
     9241 agctcgatga attaggacgc gacaacaatt ctgtcgtcgt ccagcgttat aaaggtcttg
     9301 gtgaaatgaa tccggaacag ctctgggaaa cgnnnnnnnn nnctccaagt ccacttggaa
     9361 gatgctgtgg aagccgaccg tattttttcg gtcctcatgg gcgataaggt cgaaccgcgg
     9421 cgtaaattca ttgaagacaa tgcgaaaaag gtccgtaacc tcgacttata atcaggagaa
     9481 cctatgccga aactttctcg aaagataaac ttatttaccg attccgtcat tgcccgcatg
     9541 tcaaaggtag ctgaaggctg cggggccatt aacctgtcag agggctttcc cgattttccg
     9601 ccgcctaagg ctatgctcga ccgcttggcc gaagtcgcca aggagggacc tcatcagtac
     9661 agtctcgata acggggcttc gaacctgcgc cgggctctgg ctgattatcg aaaaagcttt
     9721 accggaaggc gccccgatcc ggaaacggaa atcgtcgtta cctgcggcgg tacggaagcg
     9781 ctgatgacgg ccatgatggc catcgtcaat cccggcgaca aaatcatcct cttttcacct
     9841 ttttatgaag cctacgcaac ggatgccctg ctctgcggcg ccgagccgat ttatgtgtcc
     9901 ctcaaagggg atgattacac ctttgacggc gatgaattag aagcggcctt taaacagcgg
     9961 ccgaaggcaa tctttatttg taacccgtcc aatccctgcg gtaaggtttt ttcgaaagaa
    10021 gaactgctta ccattgcgtc ccttgtcgaa aagtatgata cctttgccgt catcgatgaa
    10081 gtctatgaac acatc
//
LOCUS       DAWDXZ010000008         9591 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_2601_length_9591_cov_10.155726, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000008 DAWDXZ010000000
VERSION     DAWDXZ010000008.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9591)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9591)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9591
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2601_length_9591_cov_10.155726"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5053..5062
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7298..7307
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2303 a         2334 c         2504 g         2430 t
ORIGIN      
        1 tggccccttg gtcaagtggt ctaagacatc gccctttcac ggcgagaaca tgggttcaaa
       61 tcccgtaggg gtcaccacat gggcgattag ctcagctggg agagcgcctg ccttacaagc
      121 aggatgtcag cagttcgatc ctgttatcgc ccaccaaatt ttattcaggg acagtgcatg
      181 aatgatatct tcatgcactg tcccttttct tgtattatga tggggtgtac cgaagttcac
      241 aatatgtacc accgtccaag gcggtttcat gggaacaaaa cggcccttgt cggttgtatt
      301 cccgatgcat ttcctttatg atggaggcat agatgtttaa agtacagaca ggaggcctac
      361 agcaatggca gatacgataa aggactttaa gacgatgcag cagaaattga atttagacgg
      421 cgccgattta tgccgcttgg ctcaggattt tcgccggacg gcggaagatg ccctggccgg
      481 taaaaagagc tctctttcgg ccctgcgtac ctatttggga ctcccgacgg gattggaaac
      541 ggggaccttc atggctcttg atttcggcgg gaccaatgtc cgggcttcac gtatcacctt
      601 atccggcgca gatggattta cgattgaaac caaggtatcg aagcctttaa gaggtgacgc
      661 ttatgactac acgacttcgt cgacgacggc agaggccttg tttgacttca tcgccaatat
      721 cgtaaaagaa gcggccggcg gcaatgagcc ctataagttg ggatttacct tttcctttgc
      781 cgtcaatcag acgtcagcca aagatgccag cctcatcgcc tggtcgaaag aaatcgatgt
      841 ccccggtgtt gccggtaagt cgatcaacgg tcttcttaag gaagccctcg ttcgcaatgg
      901 aatggaccgt attgaaccgg tggccctgtt gaacgatacg acagcgaccc tcttgtcgtc
      961 tgtctatcag cataatccgg cccagatcgg catcgtctgc ggtacaggct tcaatatgtg
     1021 ttattatgaa ccggcacttt tgatgatcgt caacttagaa gccgggggtt tttcgggagg
     1081 accgcgtacc gattgggatc gccgtgtcga cgaagaatcg cagcagcccg gagatcattg
     1141 ttttgaaaag atgattggcg gccgttactt gaacgagctc ttccgcgtca tggtgatgga
     1201 ttatttggat cgcgacgatg ttccccgttt tacgacgcgc tgtatgaatg aacttttctc
     1261 cggtgaaaag acggtccgcg acgttctcgg ctttgacgtg acgctggccc aaaaagaagg
     1321 cctctgtgcc ttgggcggag ctgtctttac gcgggcagca cagatgatcg gcgctgccag
     1381 cttcggcatt ctcggccatt tggcgggaga agaagaactt aaggaacagg ccattgctat
     1441 cgaaggcagc cttgtcgaaa aaatcgccgg cgtccccagc gttattgaag gggctgtctg
     1501 ccgttcctgc atgggtctct ctgaaggaac gccgattcac attgtgccca acagccacgg
     1561 cgcctccatc ggggccgcca ttgctgcggc tctctgcgtc tgatgccggt tcccgactct
     1621 tgacgccctt cccctaattt tctataataa aagcataatt ccggcccttg cggaaatctc
     1681 cgcaagggct tttttgcaag ggaggagcgg ccatggcact ggaggaactc atcaatgaac
     1741 accgacaaga attaaataat accgatatgg gtatctggaa gtatatcgta cagcaccggg
     1801 ctgaggttcg ccgcagttcg attcacgaat tggctcgagc ctgcaatgta tcgacgacga
     1861 ccattgtccg ctttgcgcaa aaattgggcc tcgaaggctt tggcgaattc aagctgctcc
     1921 tcaaacggga acaggcgaaa tccttcgagt atgatgccca taccttggaa gggctgaatc
     1981 gattttatgc ccaaaccgtc gataaggtct gcaatcttaa ttttgatcgg gccagttccc
     2041 tcatccatga agccaatcgg atttttacgt attcttcggg ctatgtgcaa aataacgtcg
     2101 ttcaagaact gaagcgcctc ttcttctatg acaatatttt gatttacgat gtgccggcgc
     2161 gggaagaatt ccgcaatctt gtccattccc tgactaagga cgacttgttt atcattgttt
     2221 ctttttccgg cgaatcgccg gccgtaaccg aattgggcca tctcctgaag gcgcgggata
     2281 ttccctttat ttccgttacc cgtctgcagg ataataccct ggcctcgttg tcgacggtca
     2341 atttctatat ttctccgggc cagttccaac tctacgataa ggaagaggaa cacgtcccct
     2401 tccagtctat gatgccttac tttgtcctca tcgaagtctg gtatgtcaat tactgtatgt
     2461 actgtcatcg cctgcgccaa ggtgacgaat gatatgtacc aaagtacgta ttttgtacca
     2521 gattacagcc ccgtgccgca gtgccggaaa gccgcgaaac tattgtgttc gcggctcttt
     2581 ttttatatcc tgttagtaaa gatagcttca aggaatacat cgaatgacgt cttcgctata
     2641 tgaccgagaa tgctaaggac ttatattttt attttaaacg ggaggaaact gttatgacat
     2701 tcagcaagga acgcagtatg caggaattgc agcgctttgg cggcgctatg tatacgccgg
     2761 tcattttatt tgctttcttc ggcttgactg ttgccgtgtc gattattttc agtaatacgg
     2821 gactctttgg cagcttagct gaagagggga ccttgtggta cgatttctgg tacgtcgtcc
     2881 agcaaggtgc ctggacggta tttaagcaga tgccgatcct ctttgccatc gccgttccca
     2941 ttggttttgc caagaaagaa caggcacgct gtgccatgga aggcttcgtc atttatatga
     3001 ttttcaacta cttcatttct ggcctcttga gtctccacgg cagttatttc ggcgtcgatt
     3061 acagtcagga agccggagcc ggtacggggc tggctatgat tgctaacatc aagaccttgg
     3121 atatgggcat gctcggcgcc atctttattg cctgcatttc tgcctatctc cacaatcgct
     3181 tctatgatac ggaaattccc gactggctcg gtatcttcaa aggtccggct ttcgtcgtcg
     3241 ccgtcggctt tttggtcatg ctgccgacag cccttctctt ctgctatatc tggccgccca
     3301 ttcagcacgg tatcgaacag ttccagttct tcctcaagac cagtggtatc ttcggtgtct
     3361 gggcttatac gttctccgag aagatgctcc tgccggcagg ccttcatcac ttcatttacc
     3421 tgcccttcat ttttgggccg gctgtcgttg acggcggcat tcaggcctac tggttgggac
     3481 acatcaacga ctttgcaacc agtgcccagt cattgcggga tatgttccct cagggcggtt
     3541 ttgcccttca cggcagcggt aaagtcttcg gtcttcccgg tgcagccttg gccatgtatt
     3601 actgcgccaa accggaaaag cgtaaaaagg tagcagccct cttgatcccg gctacgatca
     3661 cagctgtcct ctgcggcatt accgaaccga ttgaatttac cttcttgttc gtcgcccctg
     3721 tgttgtacgt cgtccatgcc gttttatcgg cgacgttgtc gtccatcctc tactttatcg
     3781 gcttgtctgg caactttggc ggcggcctca tcgatgcctt cgtccaaaac tggattcccc
     3841 tgttccgctt ccacgccggg acgtatatca tgcagattgc catcggcctc tgctttaccg
     3901 ctatttattt cgtcgttttc cgtttcttga tccttcactt cgactttaag acgcctgggc
     3961 ggaccgatga tggtgaagac gataaattgt ttaccaaggc ggaatataaa gccaagaaag
     4021 aaatggaaaa gaagggcatt gccggtggtg ccgatgcctt gaaagctcag gtcttcctcg
     4081 actgcctcgg cggtccggac aacattcagg aagtcaccaa ctgcgcgact cgtctgcggg
     4141 tcacggtcaa cgatccggat aaagttgccg ggacgagcaa gtttaccaat gccggggcct
     4201 ttggcttggt gaaaaacggc cgtgccgttc aggtcatcgt cggtctgtcc gtgccgaacg
     4261 tccgcagcca tttcgatgcc ttgctgaaag gcgattatac gccgattgct gaaaccgtgc
     4321 cgacagaacc gaaagcggct gaatcggatg gcactaaggc ggaacccgtt gctgctgtcg
     4381 cttctgtcga tgaagattcc cgctccatgc ggctgaaagc cttcgtgtcc ggcgacctcg
     4441 tcgatatcag cgaagtccct gatgacgcct tttctcagat gatgatgggc gacggcgtag
     4501 ccattaagcc ttccggcgat acgattgtgg ctccggcgga ctgcgttgtg tcgatgatta
     4561 tggaaagctc cctccacgcc gtgggcttgg aactgagcaa tggcgctgaa atcttgattc
     4621 acatcggtct cgatacggtc aatttgaacg gtgaaggctt taccctcttg acggaacagg
     4681 atgcacaggt aaaagcaggt gatcccttga ttcgcttcga ccgcgatgtc attgctaagg
     4741 ccggctatga cgatatcgtc atcatggccg ttaccaattc tccggaatat ccgcagatga
     4801 agaaagaacc ggaccagcct gtcgtcgctg gtgaaacacc gattgtcatt ttctaggagg
     4861 tttttatgga tacgttaaaa tggtggcaga aaacagcagt ctatcaggtt tatccccgca
     4921 gctttcagga tacgacagga agcggcgaag gcgatattcc cggcattacg cgtcatctcg
     4981 actatttaaa agccttgggc atcggtgcgg tctggttgac gccggtctat ccgtcgccca
     5041 tggtcgacaa cgnnnnnnnn nncgatatca gtgattatac ggccatcgac cgtcgctacg
     5101 ggaccctggc cgatatggaa aaactcattg ctgaaggcaa gaagcgggat atccgcatcg
     5161 tcatggattt ggtttacaat cacacttcgg accggcatga ctggttccaa gaatcgaaac
     5221 ggagccggga taacgataag gctgactggt atatctggcg tgatgccaag gaagacggtt
     5281 cggctccgac gaactggcgg tccatcttcg gcggttcggc ctggacctgg tgcgaagaac
     5341 ggcagcagta ttacctgcat acctttgccg tcgaacagcc ggatctgaat tgggaaaacc
     5401 cagacgtccg ccaggccttg tatgacgcgg ccaacttttg gctcgataaa ggtgtcggcg
     5461 gtttccgtat cgatgccatc gtctatatca agaagccggc tgtctttgaa gacggccctg
     5521 tcgatggagc tgacggcttg tcgtcgatcc atcagatgat tgccaatacg ccgggtatcc
     5581 tcgactttct ccatgaattt cgcagccagg tctttgacgg tcacgatatc ttcaccgtcg
     5641 ccgaagctaa tggggtggca ccggaagagt tgccccaatg ggtcggcgaa gacggcgttt
     5701 tcagtatgct ctttgaattc agccattcga acgtggaatt tacggatgat gaaatttggt
     5761 gcaaagcctc ttcgtggccg ctgacgaagc tcaaaaaagc actgtctgcc agccaaaggg
     5821 cgacggctga taacggttgg tatccgattt tctttgaaaa tcatgaccgc aatcgctgtg
     5881 tcaatcacta cttcccggag ggaacggata cccaagcggc ggcgaaagcc atggcgacgg
     5941 tcttgttcgg gcttcggggg acgcctttca tttatgaggg gcaggaatta ggcatggcca
     6001 atacggcgtt tgaacggatt gaagactata acgatatttc gactcatggc cagtatcaga
     6061 tggctttgga ggaaggtctt gaatcggcag aagccctgaa ggtcgtacag ttccacagcc
     6121 gggacaatgc caggactccc atgcagtgga cgacggaggc gcaggccgga tttacgacgg
     6181 ggacgccgtg gcttcccgtt catgacgact ttgcctcctg ctgcgctgaa gttgaagaaa
     6241 aagatgatca atcggtcctt tcttactatc gtaaactcca gcatgaacga ttccgggggg
     6301 aagcgtcgca gattttgctg cagggcagct atgaagaact cttggctgac gatgagtcca
     6361 tctacgcttt taaacggact ttgggtaatc aggctgtcct gatcctggtc aatttcacga
     6421 atgatacggt tcgttataat gtcgaccttg tacagggcgc ctcggtactt attgggaatt
     6481 ttgaaaatcc ccgggccggt gaattacgac cggcagaagc cgtcatctat cgtatatgag
     6541 gaggaatatt atgttaaaag ttgctgccag tgattacgac gggactttat tccgagacga
     6601 tatcattaca gctgaagatg cggaagccgt tcgtcagtgg cgggctgcgg gccataaatt
     6661 cggcgtcgtt tccggccgtg accacggcat gctttggccc cagctcaagc actacggcat
     6721 tgaatatgac tatttggcct gcaataacgg aggccttatc agtgacggac agaatcgggt
     6781 cctttgggaa gcccgtatcg agccggccgt tttaaagaaa gccagtgaac tgcctctggt
     6841 ccgaaaatct ttccatttct ctttttcatc agaagaccgg acctatcttt gccacgatat
     6901 ggaggggacc tgggtttggc gcgaggctga acagtgggac tataccttgg ttcctattac
     6961 agaagaggat atagagcact tgccgcagat cattcatcag ttttccttag ggtttacgaa
     7021 cgcggaagat gccctggcgg ctagtgagga agtcaacgct aattttggca acgtgattca
     7081 tgcgtacccc aatcgctgcg ccgtcgatat tattccggct gacatcagta aggaacaggc
     7141 catttcccat acgctcgccc tcttcgggtg ggacggcgcc gaaatcttgg ccattggcga
     7201 tgaaatcaac gacctgccca tgatcctggg atacaacggc tatactgttt ccacggcccg
     7261 accggccatt caggctcagg cccgcaaagt ctatgacnnn nnnnnnngcg taggggccat
     7321 gctgatcgat aacttataag aaaagaggtc cttacgatgt attttaataa aaaagcctgt
     7381 gcggtcttag cggccttgac gttggaactg tccggcgcgg catgggcggc atcggccgac
     7441 agtttctccg atgtaccgaa agaccattgg tcctatgaag ccctcgatta tctggctcag
     7501 gaaggggtca tcgaaggcat gggcgacaat acctttgaag gtggccgcac gatgactcgc
     7561 tatgaagtgg ccagcatcgt tgccaaagcc atgcagaagg gcggcggaag ctttggcgat
     7621 aaggcggttc ttgaaaagct ccacggggaa tatgctgatg aaatcagcgt ccttcagcag
     7681 caagtagcgg ctaacaccaa gcagatcgac cagaatacga aagatatcga tgaattaaag
     7741 aacggcatcg ccggcaagat ggaactcagc ggttttgtcc gcgtccagta cgaccatgat
     7801 aaatacagtg gtcaggtgca gaataacagc gacctgtaca aggataataa tcgcttctat
     7861 atgagcctta atggggcata taaagtaaac gacaactgga aagctaaatt ccagctagaa
     7921 aaaaattcct tctacaacgg cggtcacgac cacgagaatg aatcgggttc atgggcagcc
     7981 gacgggaaag acaagggccg tgccaagcgc tggtccggtc acgatggtaa tatccagcgt
     8041 atctgggtcg aaggaacgga tcctaagacc ggagcttgga tcaacattgg ccgggcatgg
     8101 cgcggtttag ggcagcaaaa cgtcctcttc ggaaccgaat ccgatggtgt tcaagttgcc
     8161 gtaccaatca aggggacgaa tctgatggcc agtggttttt ggatggcttc gaccggggcc
     8221 ggcaaccacg aaagttggta cggccttggc acgtggggga atgtagggcc gcgagccggc
     8281 atcaacttgg cttatgccct caactccaag aataagggcg atgtttacga aaagaacacg
     8341 acccatcttg aaacttgggg cgaagaaact gtcgattacg ataaagccta cgtcttaagc
     8401 ggctggtttg acgtctcaaa agacgtccgc ttcctggccg actatgttcg tactaatgcc
     8461 gactatcaaa acagcagtac cttcctgcgg ttgaactaca aagaaacgga cctgaagaag
     8521 ccaggaacct ggcaggctta tgctcgatgg tatcgatatg gaacgaacgg taccattgct
     8581 ggcgacgatg aatggggctc cctgttctac aatggcagtg ccggaagtaa aggctggatc
     8641 ctgggctata aatacgttcc ctggaagaat atcgaatggg aaacgctgta ctcgaaacaa
     8701 gaacagattt ctaactccga taataagcga accctcatcc gtacgcagat ggacttccat
     8761 ttttaaagta gcaagaaacg actaaatgtt ttagaaaatc ccctgggtaa tgtgaaccgc
     8821 cccccctaaa gttggaccta aaaatctaac tttaggggag gcacatcata ctggctcagg
     8881 ggactttttg gttatggaat agactttctg attataatat tgccatctta tggataggag
     8941 atatttgaga tccttggatt tcttgaataa tccgattttt attcatcaga atgctgagtt
     9001 agacagaaaa taaggtttcg tttggatata aatgagacga aaaagttttc gttgcatttg
     9061 cgtttcttta ttactgataa tgctgttcgc attactttgc cagtcctaga tgcggttcct
     9121 caaatgacac aggatgaatt gctcgtcttc aaggtgttgc cggaaaatcg cctcatgagt
     9181 cgaaaagaaa tcgaagagca aactccattc acgagagtta aagtaatacg tgtattaaat
     9241 gaactggttc aaaagaaggc catacgcaag actggggtgg gacggggaac gaaatatatg
     9301 cgggcataaa aaataagctg cttcagtgaa atcgtgtaaa tgatatgctc ctcttcaaga
     9361 aacatgcgat tttattattt ctcatgtcta ccccgaagtg gacatatcat acttttttgt
     9421 gcgaggtctg ttttgctatg ttttccttct tgacgcttct tttccctctt gatataatta
     9481 aataacacaa aacaacaaat ataaacaacg aattgtgagc gaactatgtg aagatgaaca
     9541 tgaggtgaag gatatgctac cggcagaacg acggcaattt attttaaatg a
//
LOCUS       DAWDXZ010000009         9484 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_2622_length_9484_cov_8.355181, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000009 DAWDXZ010000000
VERSION     DAWDXZ010000009.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9484)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9484)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9484
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2622_length_9484_cov_8.355181"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3094..3103
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4870..4879
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6071..6080
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7051..7060
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2221 a         2843 c         2318 g         2062 t
ORIGIN      
        1 ggttccgaca cggaccgggc ctgaagtgcc ggtgccgtct cggcctgtct ctgcagcccc
       61 gatatgatcg gaccggaagc gcaaaaagaa gatgtcatct ttaatatgac acaacaacag
      121 ctagccgatt tagttcttgt cgaaagtcac agccacggac catttccaca gatcgatatc
      181 cagcaagctg attccgcgaa tatagtcaat catatcttat tcctcacggg ataccgttcg
      241 tcggaaatat aagcccttag tgattatgta attgcatctc gcagacagaa gttctactat
      301 ggtagcatag ctttacatcc tgcccgtgat gtacaaaaat cagttgaacc caaaagcgag
      361 gtgttactat gaatactcat aaaaacattg acacagtctg tccctactgc gggtcaggct
      421 gtgtcattac ttgtacggta gacacagaaa cgaatcgtgt cattgaagca aaaggatctc
      481 cggaaggcgt caacaacgaa ggccgccttt gcttaaaagg cctttatggc tgggactacc
      541 tgaatgatcc tcaaattctt accaagcgaa tccgccatcc gatgatccgc aagggcggca
      601 aacaaagtcc cttagtcgaa gtatcttggc aggaggctat cgaatacacg gcatcgcggc
      661 tcaaagccat catcaaagag tatggccccg acgccgtcat gggtgccggt tcggcccgcg
      721 gtccgggcaa cgaaacggct tatattaccc agaaattcat gcgcgccgtc atcggcacca
      781 ataacgtcga ccactgcgcc cgcatctgcc acggcgcctc cgttgccgac cttagccaat
      841 cggtcggtga aggtgccatg tcagtcagca ttccggaaat cgaagatgcc gaagtcattt
      901 tcaacatcgg ttacaatgcc ccggtctcac accctattgt agcccgccgc atcgtcaaag
      961 cgaagaagaa aggctgtaaa atcatctgcg tcgacccccg cctgacggaa atgggacggt
     1021 tggccgatat ttacctcccg ctaaaaggcg gtaccaacat gcttctgctc aatgccatgg
     1081 cccatgtcat ggtaacggaa aacttagtcg accgcgattt tatcgaaaag catacgacag
     1141 gtttcgatgt ctatgccgaa gaaattgcac aggacaagta cgccccggaa gccgtggccg
     1201 ccgcaaccca gttagatccc gacgcgatcc gcaaggccgc ccgcctcttt gcttcctcga
     1261 aacacaccgt aaccctgtgg ggcatgggca ttacccagtt ttctcaaggt gtcgaatgcg
     1321 tcattgcctg ctccaacctc agcctcatga ccggcaacta tggccattac gctaccggtg
     1381 tcggcccggt ccgaggccaa aacaacgtcc agggaacctg tgacatggga ctcctgccca
     1441 accagtaccc cggttatcag cccgtaacgg acgaaaccgt ccgccgaaaa ttcgaaaagg
     1501 cctggggttg tgtcctgcct gacaaagtcg gactgcccct gacccgcgtc ccggccaagg
     1561 tcctccgtga aaaggacccg aaaaagcaga ttcatgctta ctacatcatc ggtgaagacc
     1621 cggctcaatc ggacccggac cttccggaaa tcagacggac actcaatgcc atcgatttcg
     1681 tcgccgttca agatattttc ttcaacaaaa cctgtgaaca cgccgacgtc gtcttcccgg
     1741 ccacagcctg gggcgaacac gatgccgtct actcctcctg cgaccgccgc ttccagcgcg
     1801 tccgcaaact gatcgagccg cagggcgact gcaaaccgga ctgggaaatt ctctgtctct
     1861 tagccactgc catgggctat cccatgcagt accataacac ggaagaaatc tgggatgaaa
     1921 tgcggtctct ctgtcccaaa tttaccggcg cgacctatga aaagctggac gccaatcacg
     1981 ggatccaatg gccctgtttc tcggaagacc caaatgataa gggaacacag tacctccata
     2041 aaggcgctca ctttgcaaca gccgacggaa aaggccgttt ctattacttc ccgtggacac
     2101 cggtaaaaga agtcgaaagc cccgattatc ccctgtcctt atcgacggtc cgcgaagtcg
     2161 gccactattc cgttcgaacc atgaccggca actgtcgcct cctcgttgac ctggccgatg
     2221 aaccgggata cattcagatg aatcccgacg actgtcaaag tctcgatatt gccgacggcg
     2281 acttagtccg agtcatctct cgacgcggac agaccatcac ccgttgtcag gtttccgacc
     2341 gagtctctca aggcgccacg tacatgacct atcaatggtg gattggcgcc tgcaatgaat
     2401 tgaccatttc cgccttagac cccaagagca gcacgccgga atataagtac tgtgcctgcc
     2461 gcgtcgaaaa aatcgacgac caaaaacagg cagaaatgag tgtaaaagcc cagtacgccg
     2521 ccattcgcag ccgcatggga attcaatcgg aaggaggcct cggccatgtt taatcatatt
     2581 aagacagatc cggaactctg tatcggctgt aagacctgca tggcagcctg cgtcgccagt
     2641 cacaccggca aggccttatt cgccatgaaa ccaaacagct tcgactttac gccgcgcgtt
     2701 cacgtcgtgt acacgtcaaa aatcaccaag gccgtccagt gccaacagtg tccgaaaccg
     2761 cgttgtctcg acgcctgccc ttttaactgc atctccctcg gggaagattc cgtcgtcatc
     2821 gatgaagacg cctgtcgggg ctgcggcaag tgttctaagg cttgtccctt tgacgccatc
     2881 accatgaccc gcatttatca cggcaatacc aagggccgcc cattccgaat cgtagcttat
     2941 aaatgcgacc tttgtgaaaa caccgagacg ggaaaaccgg cttgtatccc tgcttgtccg
     3001 acagaagcct taagtctctt cgtccctaaa gccgccgttt cggcaggttc caagaggact
     3061 ctgacaaaga tatagtcatt caaaccagta taannnnnnn nnnaaaagat atcgaattat
     3121 ggaaatcgta acgattaccg aagcaaggcg ccgtctccgc ggcgccttgt cttcctccca
     3181 aaaagaaatt gaaactgtct ccccggaaac tgccgtcggc cgcattaccg caaaagacat
     3241 gactgccgcc catccctatc cgccttaccg taaatcgccc ttcgacggct atgccctctg
     3301 cagccatgat caaaagacct acgacgtcgt cgccaccatc ggggccggcc tcgtctatga
     3361 cggaccggtt ttgccgggtc aggccgtccg cctgatgaca ggctgtgccg tcccggacga
     3421 ctgcgatacc gtcattcctc aagaatacac ggtcctccaa aataatcgga ttaaggtgac
     3481 agaaagcatt ccgcacggca gcaacatcat tcccgtcggc gaagagtgtc gtgaaggggc
     3541 cgtcatcatc cccaagggaa cgaaactgac agccggcgcc gtctccgccg ctgtcggcat
     3601 gggaaacgcc acctttccgg tcttcgccaa aacgaaaatc ttgttcctga cgtcaggaag
     3661 ggagttagtc ctccccggtc agaagcgaca cgacgggcag atttacaact ccaatgctta
     3721 ccttttccgc cccttactgg aagcgcaagg tgcagccgtt accttctatc atgtttcaga
     3781 tgcgccggaa tgcctcgacg ccgaaacgga aaaaatccgg gccttaagcc gtgacgccga
     3841 cctcatcatc agcaccggcg gcgtatcagt cggtctctac gacacgatgc ctcaaatcta
     3901 cgaacgcatc ggttccgtcc ggctttacaa ccgcatcacc atgcgtcccg gttcggcctc
     3961 atacggcggc gtcatccaaa gggataccgg caagcaaacg atggtcctcg gcctatccgg
     4021 caatccgtct gccgccttca acgccttcgt gctcctggcc ctccccatcg tccgccaaat
     4081 aaatggtgaa aaaaacagag acttgccgat cgtcacctgt cgattaagcc atgccattca
     4141 caaggaaaat cccgtcgacc gcttcgtcca agggcacatt tcctttgaag atggtaaggc
     4201 cgtctttaca ccgaatacga ctctcacgtc gagtgccctc ttaggccttg cccacgtcaa
     4261 tgccctgggc tgcatcaaaa aaggaacgcc tcccttgccg gaaggcgctc ccatcgacgc
     4321 gattttatta ttttgagatt accacctaat ccgttccccg tgagcaaaga ccttaaacga
     4381 atcgggacgc agcccacaga cgacggtaat gccgtatttc tctgccaaag ccacgcctaa
     4441 actggtcggc agggcccgcg aggccaagaa acggacgccc aggccgtgga ccttttcgat
     4501 aaccgactgc ggcacgcggc cgctgaatac taaaacggcc tgactgacgt cgatatggtt
     4561 catttcaaca aaaccgcgca gtctgtcgag aacgttatgg cggccgatat cttctgtgta
     4621 gacgaggatt tcgtcgccct ttactaaggc tccttcatgg acgccgtgcg aggcatggtg
     4681 ggccgtcgat aagctgtcta aaagtccgcc ataagagcag agctgctgtg ccgatatcgg
     4741 attaaacggt tcgtctctcg gtgcttccac gacgccttcc cgggtcgtca cccaagccgc
     4801 cccgtcatgc aggctgacgt cagataaaac tctgccgccg gcaaccttcc cctcagccaa
     4861 acaatagccn nnnnnnnnnc ggctgccaga acatcgacgt cttccggcga agatatgagg
     4921 gtactgaccc tcacaccatc gagataaagc ggcgtcatga cctcaatggc aatggcctgc
     4981 tttttgggct ccgccccctg atgataatcg acataggata aatcccggca tgatgcaaaa
     5041 tggagtgcat cttcttctct ttccatttaa aacccctcga ttcttattta aaacaaatca
     5101 tctcgcccca accaaaggag ggccgtcaat acggcgccgc ccaagcttcg tgccttatcc
     5161 gaaatggtaa agcagttgtg gtactcatgc tcacgcgggt cgatatcggc aatcttaaat
     5221 cccctggacg cgtaatagcc gtcgcgaatc aagcccctta cgagtcccgt taaggacgcc
     5281 ggcacgggaa ttcccgtcgc ttcagtaatc gccattccgt cgggaatcgg tacatccgtc
     5341 aggacagcca taggctgtcc tttttttacg cggtcgccaa tgcggaccaa gccatagacg
     5401 aaacctgcgg cgtcactgtg gatgacccgc tctttgccat agccggcaat gatccccggt
     5461 atgcccgtat tggccgcagc cgtgccttca cgtatgatgc gccctaaccg atggcctcgc
     5521 ttcgtttcaa tgacggcatc gacatcgcgg ccggccgtaa atcccggacc taaggccacg
     5581 gtatgtcgcg ccatgccgcg gttcgtcccc aagtttttct tagccaaaat ggcatcgacg
     5641 acgccccagg gttgaagcat gtgaatgctc tttccctcag gatcgacgag aagaggtatt
     5701 tcctgacgcc cccaaaccgt cgataactca tcgatcgacc gacatcgtcg acaggtcata
     5761 ttttctacga cagcctgtcc gtcatagacg gcttcacaga gagatacctg ccggcgaatc
     5821 gccgacggtc ggtctgcttc taagatgagg acgcgaaaac cggcctgaaa taaagcttgg
     5881 acgacaccgg ttgccaaatc gccgccgccg cgaacaataa tcaacgtatc cttcataaca
     5941 atcctttcta taaaagcccc tcatcctgcc aagcaggtga ggggttttat tgatttcttc
     6001 ctattttaat tatactgcat tttaagccca tcgtcaggac ttttaaaggc ttatttcttt
     6061 cggctgtacg nnnnnnnnnn ccttccaaag gaatggccgt ttggaactta ccgttccacc
     6121 tatagtaagc gccggcaaca gccggtgctg tcggaatcga cgtgatttca ccgacgccga
     6181 tggccccgta agcgacatcg acgccttcct tttcaatggc aatggcttcg acatccggcg
     6241 ccttgtcggc ccgcatcaat cccaaggtgc cgtacttggc cgtaggctta caatgatcga
     6301 gaggatactg ttcactcagg gcgtagccgc agcccatgat cgtcccgcct tcaatctgtc
     6361 cttctaaact cttgcggttg acgacgcggc ccacgtcgtg ggcggcgacc attttccgaa
     6421 tcgtcccgtc ttcattcagg atgcagacgt gggttgcata accataggca acgtgtgata
     6481 cgggattggc cacgtcagcc cccatcttat cggttttacc aagatattcg ccgatatatt
     6541 cccgaccgtt cagagcctcc aaatcgccgc ctgtaaagcg aacctgcgcc gttgtcggcg
     6601 attcctgctc aaccgtaaca ccttccaagt aatcactggg ccggcttgtc ccttcgtagg
     6661 tcaggccgtt gacacggaac aagtcccgcc gcaaatcctt cgcagctcgc cgtgcggctt
     6721 ccccggtcag tgtcgtctga cgggacccgg acgtcgtacc ggcatcgggg gatgtagccg
     6781 tatttggcat atgccattta atggcttcaa tgggaaggcc ggctgcttcg gacacgatct
     6841 gcaccaaaac agtaccgacg ccttggccga tacaggaagc cgacgaatag acatggaccc
     6901 ggccgtcact gacggcaaga cggcagcggc catagtcggg aaggccgaca ccgacgccgg
     6961 agttcttcaa ggcgcaggcg atgcccacat aagggccgct ctgatagtaa acgtctttta
     7021 cggcttccag cgtttcgaca agtcccgtcg nnnnnnnnnn gccggcggca tgggtacagg
     7081 ctctttgaag aaccgggccg ccgagggagg cataggctcc gttatcggtg acagcaacga
     7141 gtttcatcgc cgtcagtttc ccattttcat cacaggccgt cgtcaaatcc atactgaact
     7201 gatggcgctt cggatggatc agaatgcttt cctgacgcgt cagcttcact ttacagattc
     7261 gtttactgac ataggcaacg agggccgcca aatgctggac cgtcacgtct tctttcccac
     7321 caaagccgcc gccgacaaaa caattttcca cgactacttt ttcttccgga aggcccaaca
     7381 tcaatgccgt ttcccgccgc gtgtcgtaaa tcccctgatc cgacgagtaa atgaagacgc
     7441 cgtcatcgaa gggcatggcg acggcacatt ccggttctaa gaaggcgtgt tccgtccaag
     7501 gcgtttcgaa atgatgggtt accttatatt tcgacgccgc aatgacggca tcggcgtcac
     7561 cgcgaactaa atgttcatga gccagtatat tcgttcctgc cgaaggatgg accaaaggcg
     7621 ctccttccgc caaagcttcg gcagcacaag tcaacggcgg cagttcttca tattcgatgt
     7681 caacgagctt cttcgcttct gccagtatct ccggcgtttc agcggccacc ataacgatgg
     7741 cgtcgcctaa ataatgagtc gtcgtcccga cgggaatgag cgtgtcccaa tcgaccttta
     7801 aatggccgac cttgaccgaa ccgggaatgt cctcggccgt cagcacacag acgacaccgg
     7861 gcagggcctt ggccttttcc gtatggatgg cctttacaat cgctctcgga taggcactgc
     7921 gaatggcgct gccgtaacac atgccaggta aatcgacgtc atctaagtca tcggtatact
     7981 tccccgtccc cagcaccttg gtcctgacgt tgattctctg gcaatgatcg ccgacgccgc
     8041 gatacccgcc gacgacggga atgggcgtat cgttccgcag gatctccgct gccagggcaa
     8101 tgccatcgat gattttctta tagcccgtgc agcggcaaat attgtttcgt atcgccgccg
     8161 taatctccag cctggtcggc gtcgggttga cgtcgatgag ggccttgccg cacatgacca
     8221 tgccgggaat gcaaaaaccg cattggacgg caccgacttc accaaaggca tagacgaaga
     8281 cgtctttttc tcgcggtgac agtccttcga cggtcacgat gtgcttaccc tcaaggcgac
     8341 cggctttctg cacacaggcc ttggtcgcct tgccgtcaat gatgaccgtg caggtcccgc
     8401 aggcgccttc actgcagccg tccttgacgg atttcagccg caggtcacgg cgcagaatat
     8461 cgattaattt acgctccgga tcgacggcca cttcctggcc attaatccat aatttacaaa
     8521 tcctatccat aacagttcct ctttccgccc atcccggact ttcagtgata cgtctgtcat
     8581 ggctgccatt tcgcctgaaa tgcctcaagc gagggaaaat accagacagg aaatgatact
     8641 tataactcta taatctaatg atgctctcat agggcacgac gcgctttcgg ggaagcagcc
     8701 gtccatatcc gctctgccct aagaatacgt tgtcttgggc aatgatctgt ccccgcagga
     8761 atacttttga aatagtgcag tctgtcgtca tcccctcata cggggagtag ccgcaggtcg
     8821 tatggagatt gtccttgcgg atcgtccgct tccgggacgg atcgatgagg acgatgtcgg
     8881 catcacttcc cggcaagagc gttcctttct tcgggtagag accaaaaatt ttggccgcat
     8941 tagtactggt cacgcgaacg aattgaccga gcgaaagacg acctttgacg acgccttcac
     9001 taaatagcag gggcatccgt tcttctacac cggatacacc gccggggcag ctgcgaaaat
     9061 cagcggcatg ggcctgtttt tcttctaccg taaaagggca gtgatccgtc gcaacgacct
     9121 gaaccgtccc gtcccgcagg gcctgccaca aggcatcgtt atcgaccttt ttccgtaacg
     9181 gcggagccat catgtattta atgccttcca aaggcccgcc ctctgtaaat ttatcttctg
     9241 tcaacagtaa gtactgcggg cacgtttcag catatacatt ccgctgccct tcttgccggc
     9301 taaggcaaat gcggtccaca ctttcatgag ccgacgtgtg aacgatgtaa acgggggcat
     9361 cgccacaatc ccgggccgcg gataagaccc cactgactgc cgatgcttcg gcctcattag
     9421 ggcgcgtccg agcttgaccg atggggctaa gttcatctgg agacaaggcc tgtcgcaggt
     9481 aatt
//
LOCUS       DAWDXZ010000010         9069 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_2734_length_9069_cov_9.762370, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000010 DAWDXZ010000000
VERSION     DAWDXZ010000010.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9069)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9069)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9069
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2734_length_9069_cov_9.762370"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6851..6860
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7768..7836
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8239..8248
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8682..8691
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1842 a         2373 c         2480 g         2275 t
ORIGIN      
        1 aaccgacctc ctaaagttag atttttaggt ccaactttag ggggtcggtt caaacgcctt
       61 tcggactctt ttttgcatct ccggaaacgg gtcggccctg cccttcggag gcttccttca
      121 aaaaatgaca gcccgggata tataaaagta tgatttccgt aagaaagagg gaaggatgcc
      181 tggttccgtg gccgccggcg atggggcgat gaaaccgtag ttttggcgta ccgggtccat
      241 cagggacagc ctttatattt ccaatgatgg agctcatcgt gcaggctttc tacgacggcc
      301 tgccgggcca tatcgccata gactaactgg gtatgggctc cttttttcca tatcggcgac
      361 gacgggagct tcccgccggt gatgtgaccg gccagaaaat tttttaagcg ccggatgaac
      421 cacgcgggca ggcgtttgtg gtactttcgg tagcgaataa agatctggat gcggtcgggc
      481 atgatgcgaa agccttcgac gacggcgatt ttattgtatt ctttcatgag gtctaaggcc
      541 ttgattacgg ccttgcccga cggggagggc tgccagacga gctggtcttc gtcggtggca
      601 acggtcccga aaggggcttt tccgctgcag gcatagaggg tgatcaactg gcctcggggg
      661 accgtcgaaa aaggcggcag ccaggagggg atcggggaaa atgcttttag ggtcatgacg
      721 acacctccgt gaattgaatg gtgtgacggg ctgtcattgc ctttattgta cacgtccact
      781 ctgagaatac aattaaggga agatgttctc ctgttaaaac caaattagac cttgtttttc
      841 ctgaataagg tggtataata aagctatttc actttaggtt ctgtatactg caaagagagg
      901 atgattccat ggttcgcagt atgacttccg gcaacccgat cgggctgatc ctggccttta
      961 ccgtgccctt attgatcggc aacgtcttcc agcaattcta taatattgcc gacatcatca
     1021 tcgtcggccg gacgatcggc gtcaatgcct tagcggctgt cggcgccgtc gcccctgtct
     1081 atatggccgt ttttggcgtg accatcggtc tgtcggccgg tttttcggtc atcgtcggcc
     1141 agcgcttcgg cgccaaggat atggacggcc tgcgccgttc tgtggcgaca tcgacgatgc
     1201 tggcctttgc cattaccctc atggttacgg ccggtgtaag cctggccatg ccccttatcc
     1261 tgcgggtcat gaacatatcc ggtgtcctct atgacgatgc ctatcactac atgatcatca
     1321 tcgttctggg actcatggcg atgatggcct acaatctctt gtccagcatt tgtagagcct
     1381 taggggacag tcggacgccc ctttattttc tcatcgtatc gtctctcttg aacatcgccc
     1441 tggccctgtt gttcatcgtc gtcttcggct ggggggtacc gggatcggcc atcgccttgg
     1501 tcatcgccca gggcgtgtca gccgtcttgt gctttgcctt tatgaaaaag cggtttccca
     1561 tgctgcgcct gacccgctct gactggaagt ttgattgggc ctttgcctgg cagcacctgc
     1621 gcctgggcct tcccatggcg gtccagttcc tcatcatttc catggggatc ttgatcctcc
     1681 aatcggtctg caacaccttc ggaccggaga cgattgccgg cttcgtatcg gcgacgaaaa
     1741 tcgaacagtt ggccctgcag cccatgattt ccttcggcat tgccatggcc gtctattcgg
     1801 cgcagaacta cggcgccggt aagtttgacc gcatccgtat gggcgtccgc caatgttccc
     1861 tggtctccct ggccttctgc gcggcagcgg ctgttgccat gtacttttac ggccgcaacg
     1921 tcatcgcctt gttcacgacc gttcacgatg atatcctcat ggaacaggct ctcctttatt
     1981 taaagatgtc ggtcccgttc tatatttttt tgggccaaat ctttgtctac cgcaatgccc
     2041 ttcagggaat gggcatttcc agcgtgcctt tgatcagcag cctgttagaa ctcagcggcc
     2101 gcagcgtgtc ggccttggta ttggcttcga tgtggggcta cttcggcatt tgctgtgcca
     2161 gtccgatttg ctgggtcatg gcggccttgt ttacgggcgg ttcctactat tgggtcatgc
     2221 actccatgaa gaataaaagg cgctgcgtaa tcgaatagac cctgtcgtcc tcggacataa
     2281 aaaatcccgt ctccttttct tgggaggcgg gattcttttc ggttagagat gtttttcacc
     2341 ccagtgcttc aattccagca ggatgggaat catggagtgg gctttttcgg tcagggagta
     2401 ttcgacgcgg accggcattt cacggtactg tttgcgttcg atgaggccgt cttcctccat
     2461 ttcttttaag gattgggcca gcatcgtatt ggtaatgccg tagacggcgc ggcgcagttc
     2521 gccgtagcgc atgacgccct tgtcgtcgat gacgcagagc aagaggattt tccacttgcc
     2581 gccgatgata tttaagacct tttttaagcc gcagccctgg ccggcgatat tttcacagat
     2641 cgattcgtga ttcatggtat tctttccttt cgtagtccgt tttttcgtac tacctccaaa
     2701 aaaacttcgt acttgataag ttttacatac catgtataat atacgtatca aaagaaaaaa
     2761 tcaagtcatt tacagaaagg tggtcttcct catggaatac aaattcacat cggataattt
     2821 tcagcaagaa gtcttagaaa gcaaagaacc ggtcatgatc gacttttatg ccgattggtg
     2881 cggcccgtgc cagatgatgg gtcctgtcgt cgccaacttt gccgaagaat atgccggcaa
     2941 ggtcaaaatc ggcaaggtca acaccgatgc cgaaccgcag ttggcgaagg attaccaggt
     3001 catgagcatt ccgaacttcg tcttcatcaa agacggcaag gtcgttgacc aggccatcgg
     3061 tgccgtatcg gctgatgctt tgaaagaaaa actcgacgca ctcctctaag tggttatgaa
     3121 agaagggaac gccatgattt atgatgtatt gattctcggt gccggcccgg ccgggcttgc
     3181 ggcagccatc tatgccggcc gcaacaacca gtcggtcctg ctcatcgaaa agggccagga
     3241 cggcggtcaa atttcgctca cggcagatct tgaaaactat cccggccaga tgctggaagg
     3301 ggagagcggt atgtctcttt ccatgcgtat ggctcagcag gcgaagaact ttggcgttac
     3361 acgcgccctc gattcgatcg tcgaagccaa cctttccggt gacgtcaagg aactggtggg
     3421 gaaatccgat acgtatcgcg gccgtacggt catcatcgcc tccggtgcca accatcgtca
     3481 gttagggatt cccagtgaac ctgagctggt cggtcatggc atttcctatt gtgctacttg
     3541 tgatgccggc tttttccgcg gccttgatgt ctacgttgcc ggcggcggcg acagtgccgt
     3601 ccaggaagct atcttcctga cccgctttgc ccgcaaggtc accgtcgtcc atcgccgcca
     3661 tgaactgcgt gccgtcccgg ctttgcagga acaggccttt gccaatgaca aactggattt
     3721 tatctgggat tctgtcatcg acgatgccgg cggcgacggc aaactgcagt ggctttcggt
     3781 gcgcaacgtc aagaccggcg aaacgacgaa aatcgaggca cctgcagacg acggcttcat
     3841 gggcctcttc gtcttcctcg gcatcgtacc ggcgacggat atgtttgccg acgtcctgac
     3901 cttgaaagac ggctacatcg tgaccgatga acagatgcac accaacatcc ccggcgtctt
     3961 tgccgccggc gatatccgct ataaatcgct gcggcaagtc gtgacagccg ctgccgacgg
     4021 cgccattgcc gccatggaag cttaccatta cttgaaccac taatagcgaa gggcacgttg
     4081 actgaaactg ggtcagcgtg ctttttttgt tttgcagaca aagatgccct gcagctgtca
     4141 tggctgcagg gcatctttgg tgaaacgcct ctttctgagc gtctcgtttt attcgaagga
     4201 gttgatcaaa gccaggagct cttcttgatg ggccggatac cattcaccat aaggctggtt
     4261 tgctttgagg agggtttcgt tcaaggccag gaagaaagcc ggcaggtttt tcgaggtaat
     4321 cgtaccgatt tgttcggcga cgacgccttt tccgagggcg taacttccgc cggcatagat
     4381 gttgaaggcc ggttccatgg cgtcgccgac ttttttggca gcgccgcgca ggccgagggc
     4441 agccgtctgc tggacggcac agttcgacgg acagcccgag aagtggcatt tcggcaggaa
     4501 atggctgtcg acgccgcggt ctttcaaggt ttttgtgatt tcagccagca tgccgtgaga
     4561 gtcgcgcagg ccttgctggc agacggtggc accgatgcag ctgaccgagt gttcgaaggc
     4621 cgtcgtcgcc gtatcacccg ctgtgagggc agcgactttc cgggcttcat cagccgtaag
     4681 gttgatgata tacgccgttt catcggcatt gaggcggatt tcggcgcctt ctatcgtcga
     4741 gagcagccct aagaggtctt tgaaaacgga caacttcggc gtaccgccta aaggcttgta
     4801 ttcgacgtgg tacaggcctt cttgtttctg cttgcggatg cgggggtcgg cgaggaccgt
     4861 gtcgtcggca ccgctcttgg caagggccgg ttcttcgtcg atggagaagt cggggatccc
     4921 ttctttgcgg gctttttcga cagcgtcgcg gacggccttg cggaaggcgt cttcgccgag
     4981 ctgatcgagg atgaagcggg accggttctt ggcccggtgt tcatagtcgc cgtacttcat
     5041 gtagacgtcg cggccgtagc ctaagatgta gtaagggatg tccatgggat cgatgccttc
     5101 gtcgatgagg atgccgaagc gcgagccgtt gacgccgaag ccgccgccgg cgtagacgtc
     5161 gaaggtgtgg tccggtcgag cgacgaagcc caggtccttg aaggtcgcat ggccttcgtt
     5221 gtcgcggccg ctggagaagc tgattttata cttgcgcggc agtttgaaga cgggaatgag
     5281 gttgatgacg aagttggcgg cggccttaat ataaggcgtc atgttgaaat attcgccggg
     5341 agcgatgccg tggagcgggc tggccgtgat atttcgcggg ttgtcgccgc cggcgccgat
     5401 gcagtagatg ccgttctcga aacagtcctg gtagaggtcg aggacggatt gttcgttgag
     5461 atgatggatc tgcagggctt caccggtcgt gaaatggacg aggtccgtat tgtatttgtc
     5521 gagggcttct ttcaaaaaag cgatgtgagc gctgtcgaaa gccccgccgg agaagcggag
     5581 gcgcagcatg ctgctgttgg cgccccgttc gccgtagctg ccgaatttgc cggaaatgcc
     5641 tttatatttt ccgggcggca gttctttggc gtaaaaagcc tgggtcgctt cgacgaattg
     5701 ggggaagcgg ctgcgtattt cttcctttgt ttctgctgta atctgagtca ttttttaacc
     5761 accccttata aaacaatgga tttttgtctt attataacag actttaccgg tcggagcaaa
     5821 aagagaattg ccggaaaaat tgcattttct ttcaaatttg tttaacctat agttataata
     5881 taggcattga cataatctta cataaaatca ggtgatgggt atgcagttac gcgactggcc
     5941 gctgcgcaaa cggcttttgt tttctaattt cattatgatt tttattcccg tcttatttac
     6001 aggtatcgtc agcatcttca tcctgctggg tcttcaattc ggcaacatca accggaccag
     6061 tatcatttcc ttcgtttggc cggaaagcgg tcagaatatg gccatccagt tcgagctgag
     6121 ccgccttcgc gtccgctccg accgctacga cggtaatttg gcgcctttgc tgctggtagc
     6181 cgatcacttg gaggaccagg gtctcaatgt gtccatttgg aaggacggac aagatctcta
     6241 cgatacggcc ggcatcgacg gggattacct tcgccgtcag gtaacggacg gtgagccccg
     6301 aggccacgcc agcttttctt ggaccggcga aggcctgcgc ttttattacg tgtcacccca
     6361 tacggacgtc catctggccg tttccggcgc cgtgcccctg taccccgagt cggaattcat
     6421 cgatttatct tccaaagaaa tcttgaaact ggccttctat gtcctggctg ttttggccat
     6481 tttgctgacc gttgccgtcg gcatcgcctt gtcgcggtgg atggctcgcc agatcgtcga
     6541 gcccgtcgaa cggctgcggg acatggccga tgccatcagt cagggggatc tcgaccaccc
     6601 cgtgcccatc gtgtcgcgcg atgaaatcgg cgatacgtgc cggtcttttg aacagatgcg
     6661 gctccagctt cgttcggctc gggatacgcg ggaaaagtac gatcagaacc gcaaggaact
     6721 cttggccggt atctctcacg acttatcgat gccccttacg aaaatcgaag gctacgccag
     6781 cggcatcgtc gacggcattg ccaatacgcc ggaaaagaga caccattatt tgaccatgat
     6841 ccaggacacg nnnnnnnnnn gttggtacag accttgttcc tcttttcgaa gctcgattta
     6901 gggcagattc ccttccattg ggagacggtt gacatcgggg cctacgtggc cgactatgtc
     6961 ggggaacagg ggccgctcta tgaatcgcaa ggcctttctg tccgtttcac ctccgccgtc
     7021 gataaggcgg aggcacgtct ggaccggagt cagttccagc gggtccttga aaatatcctc
     7081 agcaacagtc tgaaatataa ggatcaggat aggggacagg tggaagtgtc ccttaaagac
     7141 gacggcgacc aaggctggct cttgaccgtt gccgatgacg gccgcggcgt cgacgatgct
     7201 gatttgacga aaatctttga aagtttttat cgcagcgata aggctcgcag caatgtggcg
     7261 aagggcagtg gtctggggct ggctgtggtg cagcagatcg tccttgccat gggcggcacc
     7321 atttgggctc gccatacaga accgaagggg ctcaccatct gcattcgatt cccgaaagga
     7381 gaggcataag atgaagcgga ttttaatcat tgaagacgac aagggcattg ctgaattgga
     7441 aagggactac ttggaagcca acggctttgc cgtcgatatg gcgtttgacg gcgatgcggg
     7501 ggagcagaag gcccttcagg aagcctatga cttcatcatc ctcgacatca tgctgcccgg
     7561 ccgggacggg ttccaaatct gccgcacgat ccgcgaatcg aaggatattc ccatcatcct
     7621 cgtatcggcc cggcaggaag acatcgacaa gatacggggc ctgggcctcg gtgccgacga
     7681 ctacatcgta aagcctttca gtcccaacga actcgtggcc cgtgtcaagg cccacctgac
     7741 ccgctatgaa caattgacgg cccgcgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctgt tttgacggcc ttgaaatcga
     7861 cgaaagcggc catcgcgtct tcgtgagggg gcaggaagtg agcctgccca ataaggaatt
     7921 tgaactcctc ttgttctttg ccaagacccc gggcatcgtc tttagcaagg aaaccctctt
     7981 tgaccgcgtc tggggcagtg aagccctggg cgaaacggcg acggtatcgg tccacgtcaa
     8041 ccgcatccgc gaaaaaatcg aagaagatac ggccaaaccc cgctatattg aaacggtctg
     8101 gggcgccggc taccgcttcc acggatagaa agcgattttt atagatcctt cccggtttat
     8161 tgtcagtctg ttcaaacttt gtttaacttt cctccctata ctgtagccat catcaccgat
     8221 gtatagaaag gaaaggatnn nnnnnnnnaa gaaaacggga tttttcacgg gtctcatcct
     8281 gttgacgacg gccgaatatt tctacctgtc caaggccatc atcaccgata tgaccttgtt
     8341 cgtcttctat tcggcctgcc tcatggcctt ttatatcgcc tacagtgaaa aacggccggc
     8401 cttttagtat gcagcctacg cctttgccgg tctctccgtt ttgaccaaag gccctatcgg
     8461 actcttgcag ccggggctca ttatcctgct gttcctctta tggcgccgcg atttaaaagc
     8521 cttgctgcac cttaaaatga tttccgggct aatgctcttt ttggccatca cagccatttg
     8581 gtatgtcccc atgtacctcg tgcacggcag tgattttatc agccagttca tcggcgtcca
     8641 caacgtcctg cgggcgaccg tatcggaaca tccgcgatac gnnnnnnnnn nccatggaga
     8701 cttatcagac gaaacatgaa acgcaggggt tgatttccct cgtccaacgg ctctggcaaa
     8761 gagtcctgtc acaaccggct gtcgtcctcg ccgtcatgac ggccatcctt tgttttgctg
     8821 ccaatcactt gctggtcgtt accgaccctg tcgaatcgaa ttacgccttg acggccaagg
     8881 aaatgctggc ttcgggcgac tatgtttcgc ctcgcatttt tggcaattat tggtacgata
     8941 aaccgatttt cttttattgg gaactcatgg ccgcttttaa actattcggc atcagcaact
     9001 ttgcggcccg cttcttcccg gccctcttcg gcgttgccgg catcctcttg gcctacgcct
     9061 ttacggccc
//
LOCUS       DAWDXZ010000011         9052 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_2739_length_9052_cov_9.178504, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000011 DAWDXZ010000000
VERSION     DAWDXZ010000011.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 9052)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9052)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9052
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2739_length_9052_cov_9.178504"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3043..3052
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4128..4137
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5660..5669
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1920 a         2198 c         2565 g         2339 t
ORIGIN      
        1 gacggcaaaa tatataaaga agtccccttg tcaggtcatc agggaacgga gacgattcct
       61 atcaagacgg accacggcta taatctggtc gtcgtcaaag atgataccat tggcatcgtc
      121 gaagcggact gcccggataa aatctgtatc ggcgagggct ttatcagccg tcccggggca
      181 accgtcgttt gcctgcctca taagctgctg atcgaagtca agtctgccgg gggcggcgaa
      241 ccggatgtca ttccggctca ttagaggtgg cttatgaaaa aaacgtttcg catcatcatg
      301 ttgagcctgt ttatcgctca atccttagtc ctatatatcg tcgaaggcat gcttcccgtt
      361 ccttttattg cgccgggggc taagctgggg ctggccaatt tgattacggt catcgccatg
      421 tactccctgc cgcgaaaacg ggatgtctgc cttattctct tcgttcgcgt tctcttggcg
      481 gccgcttttg gcggtgggat taacgcattc ttttacagcg tggccggtgc ggtcctcagt
      541 ttaggcgtca tgctccttct caagggagcc gggaagtttt cgattctcgg cgtcagcatg
      601 gccggcggca tcttccataa tctcgggcag gtcctcgttg cctcgctggt cgtggccaac
      661 cttaaaatca tgctgtacct gccggttctg gccgtagccg gagcgggaac ggggcttctc
      721 atcggcatta cctcggtatt taccctgagg catttgaaga aactaccaat ctataaaagg
      781 atgcaggaat aattacatcg gatatgatgg ccttagggct gtaagctgac ggagtctatg
      841 atttcgtcag ctaattttat cttatcatcc ttattttcat cacaggccac gacgacaatc
      901 caggtcacag gcccgtctga aaatccaagg aggcgcgccc gcatggatct tttttcgtag
      961 gtaaaggttg cgttgcggac tacggcgggt tttccgtcca gcgtccgttc ttcttgcgtg
     1021 gttgaaacgt tttcggctcc gattttggct aagaccgcct gtgttgtcgt atcgaccgtc
     1081 tgggcaaggt tctgtcggac ggcgtcttct tgttttaggt ggaggtcatc ggccattttt
     1141 cggacgtcga cacggttggt gtaaacggcg atgtaaagac cggcatcgtt ttcaccgtag
     1201 aggcgactgc cttcataata aggcttggcg gcgggactta cgggaatggg caggtcgaag
     1261 ctttgcaggt tgcccggtaa ttttatggaa atagccgaac cgaggccgtc gacacgtttt
     1321 tcttttaagg tgatggtata gcaggccgtc aaggtaaagg ccaaaatggg cagcagtacg
     1381 atcagaatca gaaatcgaat tcgcgtgtga atcataaaat acctcgcaga acgtagacag
     1441 aagatgatat ccgtttttct tcaaaccgct tatttacggg cttcacggaa ggcatcggcc
     1501 agtgagaaag taaggggccc ggtgacttta gtagcgtcga tgataggctg catgatgtgg
     1561 cggaattcat cgctcttctt ggataataca tatagttttg aagtgttgcg ggcatcccac
     1621 aaggcgtcgt gcatttggcc gtcgaaggtt tcgccaaggg tgctgaccgc tttatcaagg
     1681 gatatgacgt tgtcgagccc cagaagattg cagtaaatct gttggaaatt ctgccagtgt
     1741 tcgagcatcc agcggacgcg ttcatcttct ttggcattgg taattttgag acggagttcg
     1801 ctgtcgagct gacgccagtc gttttcgctc caggcataca tctcgtaatc gttgggacag
     1861 ccttcattgc accaggtgag aaagtgaccg aaggcttcag tgaaatgggg ctttcccttt
     1921 aacatttcca tcgtaatgcc cgtcaattct tggcaagtct gattcatggc cgtcgtgtat
     1981 tccggcatga cgtattcttt gtaggctgag atttcattgc cttcttcgtc tatcttgacg
     2041 gcaccgattt cgatgatttc gcccttacac gttcggcgtg cttctttttg gtctcgcccg
     2101 atggggttca tctcgaagtc gacgaatact gctttcatag gtcaggctcc tttctgattc
     2161 atgatgataa tttttattat accataataa aaaggcctcg tgcagaagat attatgcacg
     2221 aggccttgta gtgtctatct tttttgaagg ctgtctttta gaagcgatgg cttagaatgc
     2281 cgggagaatc gttccttgga agtgttcgtc gatgaactgt ttaatcgggg cagactgata
     2341 aatagcgagg acctttttgt aggtttcgtt gttttcatcg ccttggcgaa cgacgagtac
     2401 gttggcatac ggcgagtcct tcgattcaac gaggagggaa tccttctgcg ggttcagacc
     2461 ggccggaatg gcgtagttgg tgttgacgca ggccaggtcc gtgtcatcga gagaacgggg
     2521 aatctgagcg gcatcgagtt cgacgaactg gagatttttc ggattccgtt accgaagcgt
     2581 ctacggagtt gccgttcttc aaggtaatca ggccggcctg ttggagcagg agcaaggccc
     2641 gggcaccgtt ggtcgggtcg ttcggaatcg ttaccttggc gccgtcggcg acgtctttga
     2701 gatctttata tttatgggaa taaacggcca tgggcaggat aatcgtcttg ccgatggaaa
     2761 cgagctttaa gccctgtttc ttgacgacgt tatcgaggta cggctggtgc tgcatggagt
     2821 tcatatcgag gtctccgtca gccagggcct tgttgggctg gacgtagtcg ttgaattcga
     2881 cgactttgat gtcgaggcct tgtttggcag cttctttggc gacttcttcc atgacttctg
     2941 catggggacc ggcggtgacg ccgactttga tttctttctt gtcaccggag gcagcggaat
     3001 cgctggagga accgcagccg gcgacagcca ggcaggccgt ggnnnnnnnn nnaagacttt
     3061 ttttaccgtt attttcatgt aaatcatatc atttctgaaa gtaaatggaa gttgtcgctc
     3121 aaaaagaaaa gccgtcattc agatgacggc tcggattgcg attcgttcat ctgccggtaa
     3181 tataccgctg gacttggcac ctatcccgat tgggggttgc cgcagcgtca tcgggccatt
     3241 ccctcggctg ctcttgatga aagacaatgt gttgttgatg gtgtatatag taccattgag
     3301 agaatacact gtcaagtcct tttttgcttt tcttgagaat ttttttgtct catgttataa
     3361 tgaacctact catcggaggg ctttataccc gtattgtatc aggctggata agatgtcgca
     3421 gcagccattg tccggaggtc ctgcaagtcc gtatatttgc aggtcgacgt aagggcagtc
     3481 aggtctgttg tggtttttta tccggctttt tttgcgcaca tagcttcgag gtgataaaat
     3541 atgcagtttg aacgggattt acattggcag atttattcgg caaaagcctt ttactttatc
     3601 ggcggtttcg gctttacgac gtgggcgtcg ctcattcctt tcttgaaacg gaacctggcg
     3661 ataccggccg accacttggg ctttctcttg ctgggaatcg gcctcggggc cttgatcatg
     3721 atgatgctgg ccggtaccat tgcggcccgt ctcggctgcc gtcgttcctt gaccatcgcc
     3781 ggcttttgca tcgctgcctt tttgaatctc ttgtgctaca tgccggccta tgggctggct
     3841 gtcctgacgg cggtctgcct cggcgcttct ttaggcctca tggatgtcgt cgtcaatatc
     3901 aatggcatct ttatcgaacg aaaagttcgt acgcgcatca tgtcgggctt gcaggccatg
     3961 tggtctctgg gcaatttcat gggcgccgcc ttctttgcct ttttgctcca attccacctg
     4021 ccctggcagg gcgtagcctt tgccagttcg gctgtcatca tggcctgcgt tttctttttc
     4081 gggcctcact tgcacaatga tagccaggcg gctcagaaac ggccgcannn nnnnnnnggc
     4141 cgcacttcat acggcctatg ggccaaatcg ccgtcatcgg ccttattggt atgattgcct
     4201 ttctcgtcga aggcgctatc gatgactgga gcggcgtctt catgaccctt gaaaagggca
     4261 tcgatatctc ccggtcgggc ttggggctga ccttgtttac ggcttcgtcc ttcatcaccc
     4321 gcctggcagg ggatacgctg actatgcatt gggggccgcg gcgccttctt gccctgtccc
     4381 tgcccattgc ctttttggga tttatcggac tcttgacgat cagcgaaggc tggctgctct
     4441 tcctgtcctt cattttaatc ggtctcggta cggccaacac ggtacctatt ttctattccc
     4501 tcttggggcg gcagaaagtc atgcctattt ctgacgccgt ttcgtcggtc agtaccatcg
     4561 gctacgccgg catccttttg gcgccggccc tccttggctt tatcggccgg acctattcgc
     4621 tgtacgcgtc tttcagcctg gtcacggtcc tcttggtcgt catggcggcc atttccttaa
     4681 aaatcgtaaa agaataatcg ctgccgccgg aacgatgggg gcataaaaaa actacggcct
     4741 tatgagcaat ccctcataag gccgtagttt tttatgcgtc gatgactttt cccggattta
     4801 agataccctt cgggtcgagg gcccgtttga tggtcttcat gatggtcagt tcgcccttgg
     4861 gcgtgaactg ttccatgtat ttcagctttt tggcgccgat accgtgttcg ccggccaggc
     4921 ggccgccgac gctgtaggca taggcgtata cttgttgatg gaattcttcg acgtttttgt
     4981 cccactcttc gtcgctcata tccatcttgc agaggacgat gtgcaggttg ccgtcgccga
     5041 tgtgagcatt gattttaact tcaaagggat aattttgacc gatgtccatg attttttcga
     5101 tcgttccggc aatcttatga accggtacga cgacgtcgtc ggtcaggaat accttgctct
     5161 ggagttcgac tgaggtctgg cagttgcggc gcatattcca aatgcggtcg tcggcttcga
     5221 ggacgtttac agcccccgat tcttcacaga tcgtgcagac tttgtccatt ttggtgtcca
     5281 attcgtcttc ctggaaggtt tcgacggtga tgatgacgta aatgccgtcg tcgtaatggg
     5341 gcatgtcctt gtaatggcag taatcggcac agccgcggac gtacgagttg tccatgtatt
     5401 cgacactggt cggatcgata ccggctttca tcagcttggg gaccatgtgc agggccttgc
     5461 gcggatcggt gtagacggcc aggacgtcga agcggtacgg cggcaggggg atgagcttta
     5521 ccgtcacctt ggtgatgatg ccgagcgttc cttcactgcc gatgatgagc tgttccaagt
     5581 cgtagccggt cgagctcttt ttgaggatac ggccgcattc gacgatgtct cctgtcgggg
     5641 tgacgacttc caggcagtan nnnnnnnnng ggtgacgccg tagcggacgg cgcggaggcc
     5701 gccggcattg gttgccagat tgccgccgat gaggctgctt tcggcgctcg acgggtcgcc
     5761 ggcatacatg aggccgactt ttttggcttc gttttgaagg tcaatcgtgc ggacgccggc
     5821 ttcgacagtc atgtacatgc cgtcttcgtt cagttcgatg attttattga ggcggtccat
     5881 acaaaggacc aggccgccgc agacgggaat ggctccgtcg gcaagactcg tgccgccgct
     5941 gcggacagta atgggaaagt catactcatt ggccaatttg acgatggccg agatttcttg
     6001 ggcattggcc ggcaggactg cggcgtccgg cacgtggaac atggccgggt tcgtttcgta
     6061 gtccgtctgg tactgcaaga gcaggtcgcg gtcggtctta acgtatgtcg ggccggtgat
     6121 ggcctttaac ttttcaatga tttcgggcgt aataggttga aagtttttca caaaagggaa
     6181 cctcctttaa tttgtgttgt cttattctac cacgccatcc gtccttaggg aaggttttta
     6241 gaatttaaag tcgatcgttg ccgatacgcc gacgccctgg acgcggctca gattgctgac
     6301 gtccgtcgat tcgatcggga tcagggcgtt ggcacgggca atgccgttaa aggacgcttc
     6361 gagttgggcg acggccttta ctttatcgac ctgagccgtc ggtccgacaa cctggacggc
     6421 accgatgtat ttgcccgtgc cgacactgac gatgggaacg actttcgtcg cgtaatccgt
     6481 gccgacgccg ttcttcatga gcagtttatt gagaaaatcg ttgatggagt cgccgtattt
     6541 cgataccaaa acggtgatgc cgcctacctt taagatagag cctaaattga aagcctggac
     6601 ggcggtgccg ccggcaaaac cgagcaggga tgcaatggtg acagctgcga taacttgttt
     6661 tttcataaga aatacctcct aatgatgttt tgaaatggaa ctgccgcgag ccaatgaggc
     6721 ggctgcggcg aaaaagcaaa gggcagacaa caggatgaaa gagacgtgaa aggcgctgag
     6781 gacgccgtcc ttgtagacgg cagcctgttc cgtttcggtc gtcatgatga aggtgacggc
     6841 agccatgctg aaggcttgtc ccaagttgcg ggacaaggcc aggacgctcg aggcgatgcc
     6901 gtgatgcatc tttttgaccg agcccatgat ggcactgttg ttgggcgctc cgaacaaggc
     6961 tgcgcccagg ccgatgaggg ccaaaatcag gcctgcctta ccgacggaga tatcggtgaa
     7021 gacggcgaag gccaacagtc ccggtgcggt caggaacagg cccagggagg caatgcctcg
     7081 cggtccgtac ttatcggata aatcgccggc ccgcggcgaa aaaatcgcca tgaccagggg
     7141 ctggatcaat aagacggtgc cggagatcgc cggcgtgagg ccgaggacga cctgcaggta
     7201 gagggacaag agaaacgaca cggcataggt cgcgctgtac tggatgaggg cagccaggtt
     7261 ggacatggaa aaggtcaggc tgcgaaataa gtagagcggc aggagcgggt gaaaagaccg
     7321 gccttcgtgg acgacgaaga tgatcgaaag gacgagtcct gcgtaaaaga aaacaccatt
     7381 gtctgaaaaa gatgacaggc cgtataaggt gcagagaatg gcgctgaccg acaggaccga
     7441 gctgacggca ttgacgaaag gagccccgtt ttcataccat tctttttgga caggacggat
     7501 gaagaggtat gaaaggagaa ccagtacggc tgccaagaga aagatacagc gccagccgat
     7561 gaactccgtc aaggcgccgc cgatgacagg tcccaaagag agcccgagat acgtcaaagc
     7621 gacggattga ccgatgatgt ggccctgacg ggattcatcg gtcgtggcta agagcagggg
     7681 catataggat acatagatgc cggccaaggc gatgccctga agggccctca gtccgataag
     7741 gatgggaagc gtcggggcca aggcagcccc gatggatgac aaggccagga ctatcaaggc
     7801 ggcagagtac gttcttcgcc tgccgtggac gtctgatacc tttcctaaag gaagcagaaa
     7861 tgccgccgtc gtcatcagga acaaggtgac gacccagctc atgcggtccg gtgtcgtgct
     7921 gaattggtcg gccatgaccg gaatggcgat gttgatcgat gtagacagaa aagggccgac
     7981 aaagcttatc aggcaaattg aataaaaaat acgcttttct gtcgtcatcg ctgcacctca
     8041 ctcatgtcac tgtcataaat acattatacc agtatatgcg ggcagggtga attctttatt
     8101 aaaaaaacgc aatgtataaa actgttttgt ccttttgaaa aataagtttt tctcgtgata
     8161 agagatgtag aacttggaga tttatggtat agtaaaagaa aaattgttga aaaaggaggg
     8221 atttactatg gatttaggac ttcgaggcaa ggttgtcgtc gtcaccggcg gtacggccgg
     8281 catcggtctg gcaacggcca aggccttttt acaggaagga gcatcggttg ctgtctgcgg
     8341 ccggcgtcgg gaacttctcg atgaaaccgt aaaaacactg ggagaggctg tctttgcctg
     8401 tcaggccgat atgactgatg aagccgatgt ttcttccttt gcccatcagg tctatgctca
     8461 tttcggccgg atcgacgtgt gggtcaataa tgtcggagcc tctattgccc gtcagggcga
     8521 ctggtatacg ccttccgaaa tagacgctca ctacgcggtc aactttaagt ccgtcgtcat
     8581 gggcagtcaa gcggccattc cctttttaga gaaacaggga ggggtcatca tcaacgtatc
     8641 gtccctggcc gcccggtgtg cgacttcggg ccgggcgacg ctctacgggg ccatgaaggc
     8701 cgccgtcgta aactatacca ataccctggc cggggaagtg gccgccaagg gaattcgcgt
     8761 tctggccatc atgccgggct tcacccggac gcctctcgtt gctgaaacca tcgatgccga
     8821 tgaacttcaa aagcaggtca aagaatgtct cctgcgccgg cctgccagtc ccgaagaaat
     8881 tgccggcccc attgtctttc tggccagtca gcaggcggcc tatatgaatg ccgccgtcat
     8941 cgaggtctcg ggcggccgca acgtgacctt gaatcccggg tattcctacg gtgattgatc
     9001 tattttttac gaaatataaa aaaacttttt tgaaactata cttgacaaga ga
//
LOCUS       DAWDXZ010000012         8908 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_2775_length_8908_cov_7.968824, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000012 DAWDXZ010000000
VERSION     DAWDXZ010000012.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 8908)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8908)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8908
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2775_length_8908_cov_7.968824"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4393..4402
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7071..7080
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2303 a         2191 c         2049 g         2345 t
ORIGIN      
        1 tggagaggac gaagcggtcg cggtccgcca ttttcggctg tgccggatcg atatgcatcg
       61 tttcaaaata gaggacgttc aaaatatcga tgatggaaag ggatccgccg ggatgaccgg
      121 atccggcctc ggtcagcatg cgcacgatgt tgcggcgcaa ctgacgagcg cgtttttccg
      181 tttccatttt cacggtactg caatctatgt tcatacgaaa acctcctgaa tgggatatta
      241 cgtcataaca cgccggccca gaccggcgtg tctacgacta cttttttcta ttagccaatc
      301 ggatgcatga cgatttttgt ggcttcgccg ctcttcagca tttgataggc tttatcccat
      361 tcgtcgatac ctacttcatg ggtaatcatc ttatcagcgt tgattttgcc ttcgctcatg
      421 agctgcagcg tcggttccca atcgtgcgta ttctggctgc ggcttccgta tacgaccaat
      481 tctttttgaa tgatcttgga gaagtctacg ggtacttcgt ctttggcaaa taagccaatc
      541 tgtacgtact gccctttctt acggagcaaa tccatgccgg actgcatgga gggaacggca
      601 ccggtgcagt catagcagac atcagcgcca taaccgttgg tcagttcatt gacaagagct
      661 ttgacgtcat ctttctgtac atcgacgatg tattctacgc caaatttttc cttggcaatc
      721 ttcaaacggc ccatatctga agtaaggccg gtcatgatga cacggccgcc gcgggccatg
      781 acgacttgtg ccgtcaagag accgatcggc ccgggaccca ggacgaggac gatatctccc
      841 ggttttatat cggctttatg tacgccatgg taagcgcagc aggcagcttc cgtaatcgag
      901 gcctgacgag tggttacatt ttccggcagg atgtggcagc tctgggcacg ggccaggata
      961 tactgtgcca tggcaccgtc ctgctgtgtc ccaaggcctt tgcggtgaga acaaaggttg
     1021 tattcgccag cctggcaata ttcgcaggta ccgcagactt caaaggtcgt ttcactggta
     1081 acccggtcgc ctaccttgac gttggtcacg ccggggccta cttctgcaac gacgccggca
     1141 aattcgtgac cgatagtgag gcctttggcg ttgacgttgt agtgaccttc atacgtgtga
     1201 atatcactgc cgcaaatgcc gatatattct accttaattt ttacctggcc ttcgccggga
     1261 ttcggttccg gtttatcgat aacagcccaa ttacctgcgc cttcttgcat cttaacgagt
     1321 gctttcatga taactacctc ctataaaaat acacttttat gaatttaaat tctaatttta
     1381 gtggaaaata tcttaaaaat agtcaaaaaa atagatgcag ggcggtttta cgccctgcac
     1441 gggcatataa ttatgcaaac aatgcaaaga tctggaagat gatgaagttg acgaagttac
     1501 ctgcctggtc aatggaagaa atgagagcgg cgccgtcagg catcttaaac tgtgccatga
     1561 ggcccatgga cgtctggaat tcagccataa acgaagcaat gagtaaggat atgctgataa
     1621 caacgacgcc ggcgatgacg gagtgaatga tgttgccgcg ggctgcagct acgatcatcg
     1681 caacgaagaa gggaatggtc gccaagtcgc caaagggcag tacgatgttt ccgggaagga
     1741 tgaccgccat taacaaggta agcgggacga gaatcagggc cgttgcgata acagcaggat
     1801 ggccggtcgt aaccgctgcg tcgagaccga tgtaaatctt agcattttcc gagtcttcgc
     1861 caaagcgttt ctgtaaaaat tcacgagccc cttccgagat gggaatcaag ccttccataa
     1921 gaagcttaac catacgcggc atgagaacca tgacgccggc catagccatg ccgacctgga
     1981 tgattttacc gggattatag ccggcaagcg caccgatgat gatacctaag aacaagccca
     2041 tgaaaatcgg ttcgccgaag ataccgaagc gtttctgaat agaagccggg tccgctttga
     2101 ggtctttgat gaaaggaatc ttctggataa gccagataag cggaacgccg agggtggcat
     2161 aggaagctgc cgaaccggtc ggaacggaaa tcccttcgag gccgaagtat gtttccatat
     2221 acggtgccgt ccagtcagca attttcaaga tggcgatttc gaagagaaca gcggcaagga
     2281 tagccatgat gaagctgttc gacaaggcat aaacgcaagc accgcagaaa gtgaagtgcc
     2341 cgtagttcca aatatcgacg tccatcgtct tcgtcgtctt ggtgacgagc atgacgaagt
     2401 ttactaacaa accgattggg agaatcgcag cggcgatcgg cgatgcccaa gcaatacttg
     2461 ccgatcccgg ccaaccgaca tcgatagccg tcagctggat gccaaagttg gcaaccatag
     2521 cctgtgctgc cggtcccaag ttatcaacta acaaggcgac aactaagttg attcctacaa
     2581 aaccaacgcc gatagtcagg ccggagcgga aggattctgc cggtttaaca cggaacagta
     2641 agccgatgaa gaaaatgaat actggtaaaa taacggtagg cccaagagct aagaatgctt
     2701 gtaccatggg tgttaattgt tccattttat gaaacctcct ttataagaca cctctattaa
     2761 ctttttaatt cttcgataat ttttgcaatg gctttgtctt ttccaattcc cgtaaggaag
     2821 gggcggccat cgatgacggg gaatttatac tggtccttat cgacgacggt cgtcgtgatc
     2881 aaaagatcag cccgatcggc atagccgggt acttccatca ttttgcactg aatcatattg
     2941 atgtctaagt tgtgttcctt agcaattttc tgtacggcgc tgttgaccac cgtagaggta
     3001 gcaataccgg taccacaagc aattaaaact gtttttttcg tattcatgat aagttcctcc
     3061 ttatacacta ttattctgct gagttgttaa agacacgttc ttttatgaag tttacgatgc
     3121 tctgtttatc attttgcgtt ttcagatagg tcaacgcatc ttcatcttgg aaaaccccca
     3181 tgaggttctg caggagatcg atttgtgcgt gcggctcttt catagctaac atgacaacga
     3241 gatcgacgga aaccgtttct tcatcgtcac ccatgatgac aaagtcgacg ggcttcgtta
     3301 aacgcgcgaa acagatagcc ggttcattta cgtgttcggc atccgtatgg ggaatcgcta
     3361 cccctcctgc gacagtcggc aggccggtag caaagacttt ttcccgggca atgacagcgt
     3421 ctttatagct atccttgaca taccccagtt tttgcaaatg acatgctaac tgttctaata
     3481 aatcaaattt gtctttggct tctacattga ggagaacgag ctcctgctgt attgtactat
     3541 ccatatgcat cctcttttct ttatgtgata atttttatgg ttctgtattt gtgtctttct
     3601 cttgagctga ctacagtata ccaagtgaga actttccctc atagttatag ttacttccac
     3661 gcatgcggaa atttttcatt cttcatgggc cgatttattt cctaaatcga attgcggaaa
     3721 attcttacca gtaatacctt cctggaaaaa taaaaaagag agacggtttt aacaccatct
     3781 ctcttttcaa aaagtatttc gctataaaat tatgctttga ggacttctac aatttccgca
     3841 attaccttgt cttttccgat tcccgtaagg aaaggtcgac caccgatgac agggaactta
     3901 tattgatcct tatcgacgac cgtcgtcgta atcaagaggt cggcccggtc tgcatagccc
     3961 ggaacttcca tcatcttgca ctggataata tccacgttca aatcgttttc attggcaatt
     4021 ttctgtacaa ccgtgctgac aaccgtagaa gtggcaatgc cggtgccaca ggcaattaaa
     4081 acagtttttt tcgcattcat agtaacatct ccttacgttt tcaaatatta taatttatat
     4141 aggacgcagc cctgtagcag gaacttcgtg atccttatgg tatcacctcg gcaaagaggc
     4201 tcataaattg ttcaaaatcc cgaattttcc gcatttgctc ggcaccattt ccgtctttga
     4261 tgaaattata taaccgcaag aatacctttt cccacagtgc cgatttttct tgttcaatag
     4321 tcaacaaaaa gacgatctgc acctgcatgt ccccccaagg aatgggcttt tgcaaggtca
     4381 gcacggaaat atnnnnnnnn nngccgcctt gaacggcttg atccgtgaga aattccaagc
     4441 attcttcccg tgttttaaaa tctttattaa gaaagaagga gtcaccatgg aaaaagcctt
     4501 tcatcaaatc atcactgccg acttcatctt ggcgatggaa aacgacgcgt tggatttttt
     4561 cgatgtcttc tttttgcagc atgtgattga cgcttaagat tttcggcgat tccatcggta
     4621 atggtaccgt agagaagata aagtcggcat catcgatgat atcctgggtc accttgtatg
     4681 caggcaggac atcgacgact tgtatgctgt tgcggaaatg ttcccgaacc ttggccttta
     4741 agagcatcga aacccccagt cctgcagagc agacgatata cactcgtttt acttcatcga
     4801 gatccatcat gctgttccgg ctcatggcag ccccaaaatg gagcgcaata taaccgattt
     4861 cattttcatt aaactggaca ccttcgatat cgttcacaat ctttgcggca tagacggaaa
     4921 tctggaaagg cacgggatag tcatgcttta ccgtttctag aagttcattc cgtatggcca
     4981 tgtggaactg gacgcgggca atagcaatgc gcagatgcag catgagccct tctttcaagt
     5041 acgaatcgtc gctaaaatcg agaccgaact tttcatcgac cctgtccagt atggtttcta
     5101 ccaattgccg gatgtgatca tcattcaatc ccgattcacc atcgtggaac tttttccccg
     5161 tcaagaggca ctgagtcaga taaaacacct cgctaaaggg caaatcgatg gacagctttt
     5221 cgttaatact gttggcgatt ttctttgccg tatcgtgttc aaaggaaact tccagttttt
     5281 gagccagact agacgtaata gccaaggtat ggccccgttg tgaccgcaat acagataagg
     5341 caacctgtac gataaggtta tgctgtgccg tatcggtaaa aggcagattg cgaacttcag
     5401 aaatattgag caaaatatct tgatactgta catcagacgt ctgtgaaaaa atccgttctt
     5461 taacgagggg attctctact tcttgaagat aatcgacgat aaagctgcgg atatttccct
     5521 cttcaccggc gattcgcaag ccttctgttt tgtactgttc cagtgtcagg ttccattcct
     5581 ctagtttcag ccgcgtttcg gcaaggtatt tttttatcgt tgaaatacca acatgcatct
     5641 cttcagaaag atgcatctgc gtaaccgaat catcatcgag acacgctacg ataaaccgac
     5701 cgataatata attctgttgt gatacagact gtaacattcc tacctgagtt ttagattcat
     5761 cggaaagttg aagaagcact tctctcggtt ccttcacttc caatcgatag cctttattgg
     5821 gggcagcaat gatttcgctt ttaaaggact gcaaccgttc ctgtaacttt ttcatatccg
     5881 atcggacagt acgggaagaa acgccgatct gcgtggcaat gtcttcccca gtcatgcctt
     5941 ccggacgggt aatgagaagc tctataattt ttttctttcg tgactttttg tccatacaac
     6001 tatttccccc ttttgtatgg aagtttcgta tcggtatata ccgaaacagc atatatacgg
     6061 caatatcgta tataccgcaa cgctgtgtat accgtaatat tatgatatat atacataata
     6121 ttgttcactt tattataaca gtttccgtat ttacatcata gacggattta tttccgcaat
     6181 ttacggaaac ggcggtcatc gaaacgcata ggattttcac tttcaataaa tcatgaaaac
     6241 ctttgccgca tctgctttag gatgctgaaa aaaatacaaa gccactgtat cgtgaacggt
     6301 taatcgtcaa gtatacactt gaaaaaatat aatacacatt atttttgatg ccggtttccg
     6361 atgatatctt ttatacggcc aaccaatcgc cggcctgccc tttcagagac gtgaatactc
     6421 ccgtagccgt attccacaga ttcttctatg gtgtcaatca agaaggctcg gtctgtaaca
     6481 cgacgagcat cgtaaccttc gcccttcaag attttccaaa gacctcctgg acccttgtcg
     6541 ctacggtcac accagccatg attcgtctcc ctccgacggt cttccgtagg tatatagaaa
     6601 gcctttcctt atttcacata aaacaagctc tcctttaggt ctgcatgaga tatcatccca
     6661 tctcatgcag acttaaagga gagcctgtca aaatctattt ttctcccgat ttaagatcta
     6721 tacggttatg cttccaatcg gtttgccagc ccatcggtct caagaaaggt aatcacgttg
     6781 tctccaggta cagcgattat tggcggcttt tcccgattag ccactgggaa gctatgatta
     6841 gggcgatgcc gaggaggttc gtcaggttca agatgtcgcc ggtgagattc atgcccagga
     6901 taacggcagc ggctgtttct aaggaggcca ggacggggac gcggctcaga ttttcgattt
     6961 gctggaggcc caggtaatat aggatatagc ccaagacggt cgggatgagt gcgtagaaaa
     7021 agcccattcc taaaacggac ggcgtcatga ccatgggccc tccttgccac nnnnnnnnnn
     7081 gagaaggcag agggcagccc aaaagaagct ccacaggctc atgacgaagg cgtcggtacc
     7141 gtaaccagct accttgccga tgatagctgt caggccataa cagagaccgg ccatgatgcc
     7201 ccagccaagg ccccacaggg aaagggccgt caagtcgaag gtgccgccgg taacggccag
     7261 gacgcagccg acgatgttga ttaccagcgc cagccccttc aaaggtgtaa tcttttcctg
     7321 aaagaagagg gctgaaaaga cggccgtaaa aacggtggcc acattgagca ggacggcgct
     7381 gagggacacg ccgatggagg tgacggccag gctgtaaaag atattataga tgccgtgaca
     7441 gatgaggcct aacagggcgc agtaagccat gatttcaggc ttcaccttca tcgaggccgg
     7501 accgtttcgc cagcagacga aggctactaa gacgagccat gaaaacaaca cccgtaagaa
     7561 gctgatccat tcgacggatg ccccggccct ttccatatac caaatgaaga tgccgatggt
     7621 cccccacagg ataccggcca gcatgacttg tccgtttccg ttgaccttca tgacgatacc
     7681 tccttttctg tacattatta tagtcatcga ttatacttaa gtaaatctat aattctttta
     7741 tatttcatac agtattactt aatgaataag gtacaggggg taggtactat ggatacggca
     7801 cgttataaag cctttttagc agccgtcgaa acgggaagtt ttgccgctgc ggccaaacaa
     7861 ctagactaca ccgtatccgg tgtcagtcaa ctcgtagcgg ctcttgaaaa ggaagtcggt
     7921 tttcccttat tgctccgcag ccgccatggc gtcaccttgt cgccaaatgg cgagcgccta
     7981 ttacttccca tccgacagct cctcagtgaa gagaaacgaa tcgaggaact ctcctcagac
     8041 atacgcggcc tattgacggg cagcctcaac atcgccgcct attccagtgt cgctacgcac
     8101 tggcttccta aagtcatcgg ccgctttcaa agggattatc cccaaatcaa gattcacttg
     8161 atggaaggca tccgtcagga ggtcacggcc tggctcgacg acggcacggc cgacatcggt
     8221 tttctcagtt atcaggaacc catgccttac gactggattc ccctttctga cgatgaaatg
     8281 gtggccgccg tcccacccga ccatgaattg gcccccaatg gcccctatcc tctttcccgc
     8341 tgccaaaaag aaacggtcat catgccggcc ctcgggaagg acgacgacgt tttgcatctc
     8401 ctaaaaaaaa ataatctcca gcctcgtatc ggttatacga cgctggagaa tgcagctgct
     8461 atttcgatgg tcgaagaagg actcggcatc tgtatcatga accgcctcat tacccggaaa
     8521 cgggtgacgt cagccgtcct cctgcccctc gatccgccgg catccattac tctcggcatc
     8581 gcctttcttc ccggcaatgc cgcctctccg gcggttcgaa agttcgccca ttacgcgaca
     8641 aaaattacaa aacgtctacc aggagctgaa tgatgagatt ggcttcgtgg gccgcacaga
     8701 tggcgacacg aggggccatg agtccccggc cgggtttggc atttcgcgtc tgatcgccgc
     8761 atacgtagaa acggtccatg attttcttgg tctggatttc attgctgtcg ccgtagccgg
     8821 ccatgcccga ggcggagacg atatatttat cgggacactg ttccaacacg gtgtgaacca
     8881 acatcgtctt agcatcgggg ttgtcaaa
//
LOCUS       DAWDXZ010000013         8896 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_2781_length_8896_cov_9.837122, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000013 DAWDXZ010000000
VERSION     DAWDXZ010000013.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 8896)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8896)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8896
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2781_length_8896_cov_9.837122"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1605..1614
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4278..4287
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2053 a         2260 c         2469 g         2094 t
ORIGIN      
        1 ggaatttcgg actaccaaga gaaacttttt ggaccggccc tttcaagaga ccttggcgtc
       61 atgtatgccc tcgatgattt atttcagccg gaagggccgt tgaaagaggc gatggactta
      121 gcggctgatc tttatggtgc cgggagaacc tttttttcca tcaatgggac gacggcttgc
      181 attgaagcca tggttttagc agtctgccgt gacggcgatg aaatcattat tccccgagaa
      241 gcgcacaaga gcgtcatcag cggcctcgtc ttgagcggtg ctgtgccggt ctatatggaa
      301 agcctgtttg atgaaaagca ccaggtatcg ctggggccgg atgtatcgtc tttagaaaag
      361 gctgtcaagg ctcacccgag cgccaaggcc gtcgttttta cctatccgac ttacgacgga
      421 atcgccggca atttggaggc catggcggct tatgctcacg agaaaggtct ctttgtcctt
      481 gtcgatgaag cccatggggc tcacttgggc tttcatccgg actttcccaa agaagccctg
      541 caatgcggtg ccgactgcgt tgcccaaagc acgcacaaga tggccggctc tttgacccag
      601 acatcgatgc ttcattgtcg acaaggattt cccttaagcg atcgcttggc ggcgtctatg
      661 tctgtcgttc agtcgacgag tcctcattat tggttcttgg cctccctcga cagtgctcgg
      721 cagcagttgg ctcttcatgg gccggagctg ttgggacggg ccttggaact ggcccggtat
      781 gtgcgtcggg aaatcaatgc catccaaggg ctgtcgtcct ttggacggga tatcgtagac
      841 ggctctatcg tcaccggttt tgatgaaacg aagatcacca tcgatttttc cggcgtcggc
      901 ctcgacggca tcgaagcgga aaaagaactg cgaaagcagg gcatcgaagt cgaactgatt
      961 gcaggcaatc acgtattggc cctgattacc cttggcgata cgatggaaac ggcccaagcc
     1021 ctgatcgatg cctgccgggc tattgccgac ggtcgccccc gttgtcagtc cgctcccagt
     1081 gcagagccgc cccttccgat tccccaggtt gccgcagcgc cgcgaatggt ttggaacggt
     1141 cctgtggaag cgataccctt tgaggctgcc tgtgggcgta ttgctgctga aacggttact
     1201 ttttatccgc cgggcatccc cgtgttggcg gcaggagaat ttattacgga ggcgtgccaa
     1261 cagtatatcc gctataagat ggcccatggc tataaaccaa acggtccggc tgacgggacg
     1321 ctgcggacga tacgcgtaca gaaggaggat tcccatgaag ggtaaattat ttattattga
     1381 aggcggcgat ggaagcggca aggcgacgca gaccaaactc ttgacggaac atctgaagaa
     1441 agacggctat cccgtcatgg ccgtgagcta tcccgattat gacagcgatt ccagtgccct
     1501 cgtgaagatg tatttgcgcg gcgacttcgg ccgcgatgcg gacgccgtca acccctatgc
     1561 cgcgtccgct ttttttgccg tcgaccgctt tgcatcctat caaannnnnn nnnnaaatga
     1621 aatggaaggc cttttatgat cagggcggta tcgtcattgc cgaccgttat acgacgagca
     1681 atatgctcta tcaaatgatc aagatcgatg atgcggcccg gcgtaaggcc taccttgact
     1741 ggctcgaaga ttttgaattc cgtaagatgg gactgcctga gccggatgct gtatttctcc
     1801 tcgacgtacc tttagcggta acccaagggc tcatggctga ccgcgtcgga aaaacaggcg
     1861 gcgagaccgg agatatccat gaaaaaaacg ggcagtttct ggccaaatgt cacgccgctt
     1921 atgatgaatt ggcgactcgc tatggctggg tgaggattgc ctgcgccgaa ggcggacaac
     1981 tccgtccgat agaggaaatt catgacgatg tataccgctg cgttttggac accgggattg
     2041 cccttgtcaa aaaatagatt cggtcggatg gagacctgtc aggcgttgaa aatactagac
     2101 tttagaggga ttttttaata taataagtta ttaaggtatt tcttaatagg gaggtgaaaa
     2161 tgatggatga gaccttgttt gatacgatca tcggccatgg ccggttgaag cgaaaattgg
     2221 ctcgtctcat cgatgaagac cgcctccctc atgccatgat tttcgccggc cctgccggta
     2281 tcggcaagac tctgatggcc attgccgtcg cctccgcctt ggccggccgt cccgtctttc
     2341 gcgatttggc accggcaggc gatgaagccc ttattgccga cggcgatgac gttttttatt
     2401 tggctcctat gggtgccatg ttgaaggtcg atcaatttcg ccagcttcag gcgaaactgg
     2461 ccctccaagg cgaacgaggt cggcgccgga tttgcattat cgaccacgta gagacgatga
     2521 atgtggaatt tgccaaccgc atgctgaaga ccttggaaga gccgccgtcc ggtgtctgtt
     2581 tcattctggt gacggatcag cctgatttgc tgttgccgac gattctgtcg cgctgcgtta
     2641 cccttacctt cgatcccgtc ggtgacgatg aaatgaggga agggctgcag cgtcttcatc
     2701 ggggcggcgg taatttggac gacgccatcc tgtggggcgg cggtattgtc cgcaccgttc
     2761 ttcgttatct cgacgggcat ggaacggaga aagcacactt tgctgtggac ttcttaacga
     2821 ttatggccaa acatgcctgt ccttattcga aatggcttgt cgtatcggct aatttgaccg
     2881 atagtgatac gacggaaatc ttaggctgga ttagtctgtt gctgcgggac atggccgttc
     2941 tccgcagcgg agccggccgg gaatatgtcc gtttaaaagg ttatatctcc gatatgaccg
     3001 aattactgcc gtactggacg gatgatgccg tctttgccgt gtcgtcagtg cttcatgagg
     3061 cgctggaagc ggtgacgcgc catgtcaatg tccgcttagt ttgggactat gtcagtctcc
     3121 agacgatacg gctcaaagga ggattttaag tgatcgttgt aggtgtgcgt tttaaacctg
     3181 ctggaaagat atattatttt gatcccgata agattgagct cgccttggat gatggagtca
     3241 tcgtagaaac ggcgcggggc atggaattcg gcaatgtcgt cattccgccg cgggacgtcg
     3301 aggattcgga agtcgtccag ccgctcaaac cgattatccg caaggctacg gccaaggatt
     3361 tgcgccaagt tgagcgaaat aaagaacggg agaagaaagc cttcggcatc tgcctgacca
     3421 agattgccaa gcataaactg ccgatgaagc tcatcgatgt cgactatacc ttcgacatgg
     3481 ggaagatcat attcttcttt acggccgacg gccgtatcga cttccgcgaa ctcgtccgcg
     3541 atttggcttc cgtctttcgg actcgtatcg aactgcgtca gatcggcctt cgcgatgaag
     3601 ctaagatgat cggcggcatc ggctactgcg gccgcccctt gtgctgcgcg accttcctgg
     3661 gcgactttaa gcccgtgtcg atccgcatgg ccaaggggca gggcatgtcc ttgaacccga
     3721 cgaaaatttc gggtatctgc ggccgcctca tgtgctgcct gcgctatgaa aaccagctct
     3781 atacatcggg cgagctgaaa tgctgcaact gccagcagaa aggctgtgag cagcagaaac
     3841 cgccggctgt gggaaaacgg gtcatgactg atgaaggcgt cggcacggtc ctgcgggttc
     3901 atatgaaaga taagactgtc aaagtacagc tcgaatcggg ccataatatc aatgtatcct
     3961 ggtctgatgt aggggaagtg gatcatgaaa attgagcttg gtccccatga acggctggat
     4021 gatctggtcc tcgacgggat gaaggtcatt cagcgggacg atcagttctg tttttctata
     4081 gacactgttt tattagctca ctttggcgcc gttcctcggg gaccggccct cgatttgggg
     4141 acgggcacgg cggccattcc cttgattttg acggctcgcg gggcagggaa tttgacgggg
     4201 gtcgaattta accccgtcat ggccgatatc gccggccgca atgtggtctt aaacggccgg
     4261 gacgaccgca tccgcatnnn nnnnnnncca cgtacaaggc gattaacccc gaattcggga
     4321 atggtcgccg tcaggtgcct tttcagccgt ctatgccaat ccgccttacc gggaaaaaag
     4381 ccgcggggct tacagtgacg tcgacggcat ccggagggca tgccatgaag aaacagcctc
     4441 cttagacgac gtcctcgatg cggcgaaata cgccttgaag tatcacggcc gttttcgcat
     4501 ggttcatatt accgaacgga tggcggatat tttagaagcc ctgcgggccc gcgatattga
     4561 accaaaggtt ttgcagttga ttcatggtcg ccccgataaa gaagcgaagc ttttcctcgt
     4621 cgaaggcatt cacggaggcg ggaagggcct gtctgtcctg ccgccgctca tcgtccataa
     4681 ggccgatgga agttatagtg acgctgtgga tcgcttatat ggaagataag gcggtgtcct
     4741 ttaaagccgt cttttttctg tgaaaggagt attatgccgg aagtttttca aaaggggacg
     4801 ctctatctct gcccgacgcc aatcggaaat ttagaggaca ttacgtaccg cacggtacgc
     4861 tgcctgcgag aagctgacct cattgccgct gaagatacgc gtcatacgaa acagctgctc
     4921 atggcttatg atatcgatac gccccttacg agctatcacg aacataataa agccgaaaaa
     4981 gggccgcagc ttatcgagaa attgaaagac ggcctgatga ttgctctggt cagtgatgcc
     5041 ggcatgccgg ctatttgcga ccccggcagt gacatggtcc gtttggcctt ggaagccgat
     5101 atttccatcg tgcctcttcc gggggccaat gccggcctta cgggactcat cgcttccggt
     5161 atggatacga cgaggttcac ctttgtcggt tttttgccga aaacccagaa acatcgcctg
     5221 ccggcaatcc agtccgtgga agcctatgag gggacgctta ttttctatga agcccctcac
     5281 cgcattcggc aggtattggg tgaaatgcag gaagtcctgg gcgaccgaaa ggccgtcctc
     5341 tgccgggagc tgaccaagcg gtatgaacag tatctccatg gacatctcag cgaccttcaa
     5401 gccgcccttt ccgagcaggg aacgcgcggt gaattcgtca tccttgtcga agggatgaca
     5461 aaaacggccg aagaggccaa cactgaaaat gttgattatg cagccctggt ccgggaactg
     5521 atggaacagg gcgttgataa gaaagaggct gtccggactg tcgccagacg gtgcggcgtg
     5581 cccaaacgta gtgtatacca ggctgcgctt agtttataac accggctcat ttgtcggtat
     5641 aggagggaaa atcccatgtc tgaaaaaaga aagtattata tcaccacacc gatttactat
     5701 ccaagcgcca aacttcacat tgggcacacg tactgcacga cgattgccga ctcgctggct
     5761 cggttccacc gcctgcgcgg cgatgacgtc tttttcctga ccggttctga cgaacatggc
     5821 cagaagattc agcgggccgc tgaagaaaaa ggcgtgaagc ccatcgaata cgtcgatgaa
     5881 atcgtatcgc tcttccaaga gttgtggaaa aaactgaata tctccaatga tgactttatc
     5941 cgcacgacgg aaaaacgcca tgaaaaagtc gttcaggcct tgatgcagaa atcctacgat
     6001 aatggcgata tctataaagg cgaatataag ggctggtact gtacgccctg tgaaaccttc
     6061 tggccggaaa ataagctgcc cgataagtgg ctgaaattta ttgacgaaaa ccctgatttc
     6121 atccagccgg aatcgcgacg caacgaaatg atttccttcg ttaaaagcgg gctcgaagac
     6181 ctctgtgttt cccgtacgtc cttcgactgg ggcatcaaag taccttggga tacgaagcac
     6241 gtcgtctacg tctggttcga cgccttggtc aactacgtaa cggctgcagg ctacctcgac
     6301 gatcctgaaa agttcaaaaa attctggccg gctgacctcc atctggtcgg taaggaaatc
     6361 gttcgcttcc acagcatcat ctggccgatc atgctcatga gctgcggcct gccgattccc
     6421 aaaaaggtct tcggtcacgg ctggctcatc gtcgatggga cgaagatgag taaatccttg
     6481 ggaaatgtcg tcgatccaat tcctttgatc gaccgctacg gcgccgatgc acttcggtat
     6541 tacttactga aagaaatccg cctcggccaa gatggtaact tctccttgcc gtcctttatc
     6601 aaccgcatca attccgacct tgccaacgac ttaggcaacc tcttgcagcg taccctggcc
     6661 atggtcgaaa aatacgaagg cggcgtcgtc gccggtccga cggtgaaaga acccgttgac
     6721 gatgatttag cagtctgcgc cgttgaaacg gcaaagcagt ttgaaacctt tatggacgac
     6781 atgaagatca acgatgccat taacgacgta tggacgctta ttcgccgcag caataaatat
     6841 atcgacgaaa cgacgccgtg ggtcctcgct aaggatgaag cgaaggctca gcgcctgcag
     6901 acggtcctgt acaatttgat cgaagcacag cgtatcatcg cggccttggt cagtcccttc
     6961 attcccgtca gcgctgccga tatgtggcat cagttgggtc ttggtgattt ttccaaggca
     7021 aaccttcagg atgcccagca gtggggcctc tatccggctg atacgaaggt tgaaaaaggg
     7081 caggctctct tcccgcgtta cgacttagac gaagagattg ctgaagctaa aaccgacaag
     7141 aaggaagaat cagctccggt ctgcccggca gctcaggaac cggtaaaacc ggaaatcacc
     7201 atcgatgact tcggtaaagt cgaccttcgc gtcggcaaaa tcgtagcctg tgaaaaagtt
     7261 cctaaagcga aaaaactgct gaaactgcaa gtcgacctcg gtgtggaaac gcgccagatc
     7321 gtcagcggca tttccctgta ctataaaccg gaagacctta tcggtcactg cgtcatcgtc
     7381 gtcaccaact tgaagccggt caaactctgc ggtgttgaat cgaaggggat gctcctggct
     7441 gcctccgacg gcaacgataa tctgcaggta gcctttgtcg acggcatggc tcccggcagc
     7501 cgcgtacggt aatgcttttc gatacacact gccatctcaa tgacagtgct tacgatacag
     7561 agcgccggga aattctttct cgggcggccg cggccggcgt cggcttgatg ctctgtccgg
     7621 cgacggatat ggcgacgtct cgcgcctgca tcgacttggc caattcttgt gacggagtct
     7681 atgccgccgt cggcattcac ccggaactgg cggctacggt aacgccggaa gatgcagaga
     7741 gacttcgtca atgggtgcgc ggtgagaaaa aggtcgtcgc tatcggcgaa gtgggcttgg
     7801 actaccattg gccggagccg gagcgttccg ttcagcaggc cgtgttcatc gacatggtga
     7861 ggctcgccgt cgagctcgac gtgccgatta ttatccatga ccgcgaggca cacggcgata
     7921 cgttggatat ccttcgccag tacggcaagg gcatccgcgg cgtcttccat tgttactccg
     7981 gaagccttga aatgacggct gaactgcttc gtatgggctt ttactttggt tttgacggcc
     8041 ccttgaccta tccgaattct aagcgggcca gacgcgtcgc tgaaaacctg cccttagaga
     8101 gaatcctcat cgaaacggac agcccgtacc tgacgcctca ggcccatcgc ggccagcgca
     8161 atgaaccggc ctatgtccgc tatgtggcag aagaactggc ggcccttcgc catataagtg
     8221 tggaagaagc ggctcgtatt acttcggaaa atggtcggcg gctgtttaat atttaaatag
     8281 tataaactgt ggacgcgggg cggatatcat cgttgatttc ccgtcctgcg tctttttatt
     8341 ttacggaata tgagaaagga gctgtcgctt atggaaagag gaaagagacc aaaaatcgaa
     8401 ttgagtgtta cgggaaaatt agggcctatg ggctgtcatc gcggccatca tattggcgaa
     8461 acctttgatt atgacaccga acggggaaaa atctgcccca tggccatgca ctgtgccttt
     8521 ccctatatcg atattctgcg ctatggcggg acacttccgg gacagcctga aggagaagcg
     8581 gaattctgct gctctgatgc cgacgtggcc ctggtgttta aggctaaggt cgtcgagtaa
     8641 gggagaaaat ttttattgaa aaagggaacg gaaaaatgct tgacaggagt tactttattt
     8701 ggtatgatat tcaagtgttc tgaaacagac acgatacatg cggaagtagc tcagtggtag
     8761 agcaccacct tgccaaggtg ggggtcgcga gttcgagcct cgttttccgc tccatttatt
     8821 cctcaatagc tcagttggta gagcaatcgg ctgttaaccg atttgtcgta ggttcgagtc
     8881 ctacttgagg agccat
//
LOCUS       DAWDXZ010000014         8401 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_2891_length_8401_cov_9.658998, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000014 DAWDXZ010000000
VERSION     DAWDXZ010000014.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2891_length_8401_cov_9.658998"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    211..319
                     /estimated_length=109
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2347..2356
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4480..4489
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2185 a         2146 c         1935 g         2006 t
ORIGIN      
        1 tacgaggaac aaagattcat catgacgacg attacatttt ataaagaaaa gaatccgtaa
       61 aggttctgaa aagtaagagg gccttaaatt tgtgaaagtt ttcccatcaa aggtcaatac
      121 aacaaacaaa aaaaggcccc gcattatagc acaaagcgta atgcggggtc tctgttacag
      181 gttattagag ggccttttcg ataatcggat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnc ctggctgccg tcacggctga cgaggaattt cgtgaaattc
      361 catttaatgc cgttgccttc ggcgttgtcc ggatagtgtt cagctacgac ctgctgaatc
      421 aggcggccgc tgtctttatc cgtttcatag cctttgaagg gagcagcttc ggtgaggtat
      481 ttgtaaaggg gatctgcctt ttcaccgcga acgtcaattt tggcgaacag cgggaaggta
      541 acgccgtggt tcaggcggca gaaatcttcg atatgtttat tatcttccgg atcctgttcc
      601 aagaactggt tgcacgggaa accgagaatg actaagccct tgtccttata ttttttataa
      661 agagcttcca agccatcgta ctgcggcgta aatccgcatt tgctggccgt gttgacgata
      721 agcagcagtt tgccttcata ctggctcaag gcaacgtcgc cgccgcggat atctttaacg
      781 gtaaaatcaa atactttcat tgtgttattc ctcctgtttc tcttagacgt catgaattac
      841 gcagccacca aattccggtg aacacatagc gctgtcaagg gccggagctt ctttaacgga
      901 gaaagtttcc ttccacaggc cgtggaggtt gcagtatgca tagacttcgc cattatcgcc
      961 ggtttcaaaa tgagccttcg gttcttcacc cggtttgaga taatggatat ctacaccttt
     1021 atcgtcgacc aaggcaatcc attcaatata atgtttttct tccatcggat gaatgacaga
     1081 accaacctga acttccagat ggttgtcgat tttctggata caggggacgt gtttttcctg
     1141 tgcggcatca acggaaccag ctacgagatg agtcaatttt tggcctgcat aaattacttc
     1201 ttccggtgcc tgtgcgccga cgacgaccaa aactttacca tctgcatcat tgcggtaaaa
     1261 ttcaacatgc ttcatatcaa ttcctcctcc aggggatagc acccctcaat caaaaataaa
     1321 aataagctat ataatctaaa aaaattataa gcatttgctt cctaattaat actttttctt
     1381 atgtgcttat tataggtcaa attgtcatat ttgtcaataa cggagaatta ttattcttta
     1441 tataagtaag tgcagaaaga ccttacagac ttccattttc gatgatttta tgcaggtttt
     1501 acgggcttta ccgcccattc cgaaaagaca gatttcttat tataatattt ttttctccca
     1561 ttttaccact cgcctttcat caaaattccg tcgtatcgtt ccgcaacatg aattcggcaa
     1621 aggcagcctc cctccataaa atgaacaagg cctccacggc tgtgcataga aaagccgccg
     1681 caacatggct gttgcggcgg cagggctaac tttttggggt cacatcacaa cagttctgct
     1741 gcggcggccg ggcttttttt atatcagaat aagaaacgtc gttgtacatg cagatacgtc
     1801 aaaggcagtt acactccgtc aacgagatga gctgcatcca tctcgggaac gacatcataa
     1861 tggcggacgc ccctttcttc ggtacagcgg agaagtccct tatccataag atactcgaca
     1921 tgagacaagg tttctcctaa ggcaaaccac ttctgcgtcg gcggaaagtc tttccagggt
     1981 ttgccgcgca tggaccaagt catatggggt gcaatctcat aggccgtgct atgcggatat
     2041 tccgcggcca ggcgaaggac ttcacggagg cgtcggtcgt gatgagccag aagttcatcg
     2101 atacggccga cgaccgacgt atcaccctcc cgatgagccg gcaaagcttg gctgaccggc
     2161 aggtccttca ccttcttcag gctttccaaa taccgctgca gtgcctgctt catatggtac
     2221 caaacctgga tattcggcgt aatgtcaaac aggatgtggt cgctcgtaaa gaaaatcttt
     2281 tccttgggca tatagaggca gcacaagccc ggcgtatgcc ccggtgtatg aatgaccgta
     2341 agctcannnn nnnnnngtcg acgggatgca tgtaaatcgg gcagcccgct ttggcaaaga
     2401 agccgacaag gccggtatga tccgcatgga ggtgcgtcaa aaataaggac gtgtggcgga
     2461 gatcaatgcc gagctcttct aaaccgcggc acagggcctc acggcattcc ggacgattaa
     2521 agcccgtatc gatgaccaga ttctcgctct tacctttaat gacataggca tttaaatact
     2581 ggagcggatt atccggtaat acgacgcgga tgcggtatac atccggcgac gcatgcagtt
     2641 tttcaatcat aaggtccctc ctaaaggtac gtagaaaaag agactgccgc agacgcggca
     2701 gtctcttgta ctcatagttt ctttttccgc ggattgtagc ggttcatgcc cttgtcgaca
     2761 ccgaggcgca gacaaccttc aatggccgtc gcggcatctt cgatgccctg gagatacttt
     2821 tcacgcagtt cttccggaaa gggcgtcaag acgtgatcga tgacatcgtg atgagggagc
     2881 gggcggccaa taccgacgcg aaagcgggta aaatcggtgc agccgttggc aaagagactc
     2941 ttaatcccat tatgtccgcc gtcactgccg ttcttacgaa tgcgcagacg gccgacagcc
     3001 aaatccatgt cgtcataagc aacgtaaaca tcaggctgct ctaccttata ccaacgcatc
     3061 agaggcccca cggcccggcc gctgtcattc atgaatgtct ggggcttgac caagatgatt
     3121 ttttcatcgt caacggtcac ctgggccacg agggcatcca ttcgctcctg ccaggacgtc
     3181 acttcccagc gttttgccag ctcatcgatg acatcgaaac ccgtattatg ttttgattgt
     3241 tcatattgac ggcccggatt gcccaggcct acgatcatat gcacggtatc gtttccttat
     3301 ttatcgaaaa gacggctgac agagacgtca tggaagatgc gcataatcgc ttcacccaaa
     3361 aggggagcta cagaaaggac cttgatcttg tcgctctgtt tttccggcgg caagggaatc
     3421 gtattggtaa tgatcaattc ggagatgacc gatttctgaa ttcgttcgac agccggatcg
     3481 gtgaggacgg catgggcgca ggctgcaaat acgcgggaag ctcccatttc cttcaaggct
     3541 ttggcaccgc cgcacaagga acctgccgta tcgacgatat cgtcgacgag gatacaggtc
     3601 ttgcccttga tatcgccaat gatattcatg acttcggcaa cgcccggacg aggacggcgt
     3661 ttttcgatga tggcaatagg cgcactgacc cggtctgcca ggtcacgggc acgggttacg
     3721 ccgccgaggt ccggcgatac gacgacgagg tcacccaaat cggtttcttc ttttttctgg
     3781 ttaatatacc gagcgatgat ggaagccgaa ccgaggtgat cgacagggat atcaaagaag
     3841 ccctggatct ggccggcatg gaggtccatc gtaacgacgc gggtgattcc cgctctggtc
     3901 attaaatcag caaccagttt cgaggtaatc ggttcacggc cgcgggtctt gcggtcctga
     3961 cgagcatagc catagtacgg aacaacggcg gtaatatgtt ttgccgaagc gcgtttcagg
     4021 gcatccgcca tgatcagcag ttccatgagg gtatcgttag ccggctggca ggtcggctga
     4081 atgacgaata catccttacc gcggacgctt tcatcgatca tgacctgaat ttcaccatta
     4141 ttgaacttac cgacaaaggc cttgcctaat tctaatccaa ggtactcaac aatttctttg
     4201 gccaattccg gattggcatt ccccgtaaaa atcttcagct ttttcccgtc ttcgtaactc
     4261 ataatgagcc tccctgtatg aatattgtaa tatatgattc gatataaaaa ccgataaacg
     4321 ctaggtggag aaagtgttat ttcttatagg tatcgtcttt aacccagtct tcgatattgc
     4381 gctgccgagc gcgggcaacg gataaggatt tggccggtac gtctttggta atcgtagaac
     4441 cggcaccgac ataggcgtaa tcaccgaccg taaccggcgn nnnnnnnnnc tacgaggtta
     4501 ctgttgcagc cgacaaaagc gtgattgccg atcgtcgtgc ggtgcttgat ttttccatca
     4561 taattgacag taatcgtccc gcagccgata ttgacgttct gaccgacatc gctgtcgccg
     4621 atataactga ggtgaggcag ttttgtgccg tctccgacag tcgagttttt gacttccata
     4681 taatttccga cttttacgtg attaccgata accgtatcgg ggcggaagtg aacgaaaggc
     4741 ccgacttcac agccgttttt gatttggcag tcatgagcat acatgaactg aacatggtca
     4801 tcatcgccca tctgtacatt ggtcatgcgg acataagggc cgatgtggca gtcttcgccg
     4861 atgaccgtct gtccttcgag aatcgttccc ggataaagta ccgtatcgcg gccgatggta
     4921 acggtcgtgt cgacataggt attattagga tcaataatag aaacgccatc ggccatgagc
     4981 tggtccagtt tacggcgacg cataatttgt tctgcttctg ccatttgctg acgggagttg
     5041 acccccagcg tttcatcata ggacggagct gcaaaagcac tgacaacgcg gcccgacgat
     5101 acgagcatgc cgatgatatc ggtcaagtaa tactcgccct gagcattgtc attggtaacg
     5161 ttgtgcagca tatcccacaa aaggggcata tcaaagcaat aaatgccggc atttacttcg
     5221 tcgacagcca gttcttccgg cgtgccgtct ttttgctcga caattttgac gacctgccct
     5281 ttatcatcac ggataacgcg gccgtagccc gtcggatccg gcatatgtgc cgtcagtacc
     5341 gttgccgcag caccactctt ttcatggcag tcgatgagag ccgtaaaggt ttccgtcgtt
     5401 accaaaggag tgtcgccgca ggtaacgaga agtgtaccgg aagcgccttg caacaacggt
     5461 tccgcctgca gcacagcatg accggtaccg agctgttcct gctgtacaac cgtttcagcc
     5521 cggtcaccga ggtagtcgcg gacctgttct ccgccaaaac cgacaacaac aatttggcgc
     5581 gtcgttccgg ctgccttaac agcccgaagc acctgttcca ccatcggaac gccgccgact
     5641 ttgtgtaaaa ccttggggaa cttcgatttc attcgcgtcc ccttaccggc agcgaggata
     5701 agtgcaatag tatttttcat tcagcaatag cctccctgaa ttacatcact atataaatcc
     5761 ttttaaggat accacaaaac gaggcgaaaa aaaaggctgt tgcacaactt actgtacaac
     5821 agcccctttt cagctaatgg atatcctagc atcaacttga gattaaccta atacataaac
     5881 gacgacgtct tgacggccga aattaagggc ttcactagta gaatccatgg ccaagtcaat
     5941 acggtcaccg acaatagctc caccggtatc agcggcaacg gcatagccgt aaccttcgat
     6001 gtaaagctga gagccgaggg gaataaccga cgggtcaacg gcaaccatac cacgctggag
     6061 atactggccg gttgcagtgt atccactgcc gccgccatct tgagccgtat aagccgtagc
     6121 ttccataacc aaccgacgct ggtgaccttc cgtcgccgat gcagcttctt ccggaaccgg
     6181 gcgaccgctg gcttcagaga gcgcttccat cgtctgcgag ccgacgattc cgtcggcaga
     6241 cagaccatgg tctgtctgga actgtacgac agcctgctgc gtctgagaac caaaatcacc
     6301 atctgcacta ccggacaaat agcccgcatc ttgaagcata tattgaacac tctgtacgac
     6361 agagcctttc attcccattt gtacacttgc acaagccaaa gacgaaaatg caaatacaaa
     6421 gatcattgtc actaatatca acatacgatg tttcaaaata cgatttcgca aaaaaccacc
     6481 tcttcgaatg aatacaatct caagtacgtc ctgaattata gcaaactcat caccataagt
     6541 caaaaaattg gatacttaaa cctacttttt gattgtttta ttctccgttc taatcgtttc
     6601 cgaaacatca tatgtgcctt aatactcaag tatgacagat attttttagt acctagtcct
     6661 atttttctat aattattata catttttaca tatcttcgct gcctgttttc tccacaatat
     6721 ttcttataga ttgagcacct tcgacagagt cagtcagcac atagaggtag tcccctgctc
     6781 gtagacaagt ctctccatcc ggtatgatgt cgccgctgcc gcgctttacg tcaacaatga
     6841 ccgtatgaga cggccaggca atgcggccga tatattttcc atcggcctga cttccactga
     6901 cgacggtcaa ttccaagggg tttcgccgtt cactcgacga taaggcaggt ttatgcttgg
     6961 ccaggctccg ccggagcagc acttcataaa tcggttcccc ccgaaagagc tgggccacga
     7021 cgagggccac caaggaggcc gtacataagg ctagcaggtg ctcatatgag ccggtcattt
     7081 ccataatgag aatagtccct gtaaccggtg actgaacgga ggccgcaaaa aaagctgcca
     7141 tggaaataac gacgatgttc gacaagtaat acgatgaaat gagccccatc tgaatgaaaa
     7201 taattcccac cataccgccg gtcagggcgc ctaagaccaa catgggcaaa aagaaaccgc
     7261 cgggaacgcc gcagccgtaa ctgataaggg tgaacagaaa ttttccgatc agcaggaggg
     7321 caaacaactg aagtgacagc ggcagggtat acagggcatt aaccaactca ttgccgccgc
     7381 cgaggacgtc gggaaaaaca taccccaaca ccccggccat gagtaaggca aaggcaatgg
     7441 gcatgtaacg attgcgaaag atgggaagtt cataaaaata atggatattt agtaatcctt
     7501 tattaaatat taccccggca attccgacaa taattcctaa aattacggcc aaccacatat
     7561 agcgcagggg aaagtgcggc aagattccga aatgaaaaac aggttctcgt ccaaagaggt
     7621 agcgactgac catggtcgcc atcaatgccg ccgccatagc cggtgccaaa acgacgccgg
     7681 aaaagttgcg atgaagctct tccaaggcaa acatgacgcc tgccaggggc gcattaaaag
     7741 ccgctgccaa cccggcactg gcgccggctg tgatgaggta ccgttcctcc atccgcgtcc
     7801 gcccaaaggc tcggctcaac ccttgggctg tcacggaacc gatctgaatc gacggtcctt
     7861 cacgtcctaa agacaagccc agcccgatgc cgacgatacc accgaccagc ttaacccaaa
     7921 gtactcgcag ccagcgcagg cgcaggacgc ccaaaatgac acctttaatt tgaggaatcc
     7981 cactgccgcc tgcttgtggt tcataaatcc ccaagcgcca taataacaaa gctgccgcca
     8041 gcagcagcgc aaaccaagcc gcgtgaacag aaatgtcacc ccggctcaaa acattggcat
     8101 aaaaccactg tcgccacagg gttcccgttt ccaagcccca gcgaaaaaag ctgataaccg
     8161 ttcccgacaa taagccgact aaaatgcctt ctaaaaaaag ctgcagccga aatcgaggcc
     8221 aatggttcaa aacctttagc gccgcttcca accgtgtatc accatgcatc atattcacat
     8281 ccctttctga ttcactgtga aaattatacc acaggctcct gttgtttcgt ataataagga
     8341 tatatataat ataaatgtat atacctatcg aagcagtgtg agccaaaaaa tcagatccca
     8401 t
//
LOCUS       DAWDXZ010000015         8049 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3013_length_8049_cov_7.797223, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000015 DAWDXZ010000000
VERSION     DAWDXZ010000015.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8049)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3013_length_8049_cov_7.797223"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1045..1180
                     /estimated_length=136
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1578..1587
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2409..2418
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3380..3389
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3777..3786
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6418..6427
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1763 a         1997 c         2135 g         1968 t
ORIGIN      
        1 ccccaagaga cacggtctga gacgacacat caccacagtt atcgaggacg gcttctaagg
       61 gctgagtatc tgtatcgagg ccagccgtca aattgttcaa gctgtgaatc aagagggacg
      121 ggcgaaaata ctgccccttg actccattgg cgacgagggc taccgaatcc ggagcttcac
      181 caacacaggt actgacagct tgacagactt cttttgaaat ctcaagaccg cgcatgcctt
      241 caggaagggc cgtcccattg tgcaggacca ggatttttcc ggaacgagcc gtcatcccct
      301 gcgctgtatc ggtcacgatg gagcagtctc gtcggcttac gatcatagcg acgtccaatt
      361 ttacacggct gctgcataat ccgctgtacg taccggcagc ctgaaatcct tgcggtacgc
      421 caaagttatc ataaacctga ctcatactga ctccaccttt ctaatgtttt aactgtaatc
      481 ctgtggcctc gtcgatgccc atcatgaggt tcatattctg gatggctgca ccggaagcgc
      541 ctttgcccag gttatcgaat aaagaagtga ccatgatgcg ctggtcgttg ccggttacaa
      601 cgatttccat atcatcgtag ccgctcatgt cattagcact gataaaaccg cccttgtcgg
      661 ccccaaagtc gaggacgtgg atgaaggggc agcattcata atgtttggct aaaatctccc
      721 agacatcttt ggccgtgacc ttcttcgtca acagctggct gaagaaagga atcatgccgc
      781 tgtaataatc gtcgacgatg ggcgtaaaga ccggttcctg tgccaagtgg cagatggaaa
      841 cgatttcctt caaatgtttg tgctgctggc ccagaccgta ttggcgcggt gcgaatagat
      901 catcactttt atccccttcg tattggccaa tcatcttctt gccgccgccg ctgtagccgg
      961 tcagagaaaa ggccgtcacc ggataatcgg ccggtaccaa acctgccgcc gtcaagggct
     1021 gtaaaataga gatggcgcca ctggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgacggcttc ggcagccgca
     1201 tcatcgggaa ggcagaggaa ggcgatgtcg gcggctttca tgcaggcacg gatggcatcg
     1261 gggtcttttc ttttttcgtc agcgatggga atcaattcga tatcctgacg ctggctcagg
     1321 cgttcatgaa tccgaagacc tgtcgtccct tcatgcccat ctataaatac tttcgtcttc
     1381 acaatgcatc actcccagaa aaaacaatct atacttcaaa atagcttata ccttcatgtt
     1441 ttattgaatt ttaattcatt atagttgaat taaaatgttt tgtcaataaa atatgcattg
     1501 tttttacata attattatta taacttttcg tcacggtcct tcattcttga aaagataatg
     1561 aggctttcgt cccattgnnn nnnnnnnttg gcttcaagat aggccgtaat tccataaggg
     1621 ataaaggcct tatcggcagt atggccgaca ggcagtccct tgacagccgg cacccgggcc
     1681 agatgagcat aataatccag cacttcatcg gtcgtaaagt caccgtcatc gtgggaggat
     1741 gccgtaaaat caccatatac aatggccgac acacgggaca agagaccgct ctgccagagc
     1801 tgattcatca tccggtcgat gcggtatgag ctttcgcccg tatcttcgag caccaaaata
     1861 caccccgtgc cgtcgagggc atagggtgta ccgacgaggg atgcaagaat cgtcaaatta
     1921 ccgcctacaa taggaccttt ggccgttccc tcagtgatgg tcgtaagttt tcgtccgggc
     1981 ggcagtgcca agactccgcg ccatgattct cgttttaatc cgtcaaagaa ttgattcgct
     2041 gtataatcat tcgaaccgcc taaggtaacc atcaaaggac cgttagccgt gataaggcgg
     2101 ctcttttccc ataaggctat ctgaagggcc gtaatatcac tgaatccgac gacccatttc
     2161 ggatgggacg ccatatagtc ataatcgagc ttatcgagga tacgagccga gccgtagccg
     2221 ccattgaggc agacgatggc ctttacgtca tcgtcatgga ataagtcgtt catatcccga
     2281 gcccgcgctt catcagttcc ggaaaagaaa ccgtaggaac cggacgccgt cggcatgacc
     2341 ttgacccgat agccggcctg ctccagacgg gcaaccgccc ttgaaatatc ctgcccctct
     2401 ccatgcgtnn nnnnnnnnat gcgtggccgg cgcagccacg ccgatatagt caccggcttt
     2461 cagcggtgcc gggatgtgaa cggccttttc cgttactact cccccttgta gtagcgctcc
     2521 attccctgag catagccgta gtcatcgttt aaatactggc ccgtcttctg gcggtagcag
     2581 aggttgaccg tcacgaagtc ggcaatgtcc ttatgcttca gctgtttctt ccgcatggga
     2641 atttccagcc aggtattggt ggagtcattg tagatccagg cctgtccgcg gaaaatttta
     2701 taggtcaagg gataggttcc ttcgatgcgt cccgaatctt ccggcgaacg gaagaggttg
     2761 accttgaaaa tccagccttg tccattatct ttgatcgttt caagggagct gaggtcgacg
     2821 tacatttcat agcccatgcc gaagttgatg ggccggtaat tgctgttggc cgctaagggg
     2881 ctgttaaaaa aggcttgaga gccaaaagca ctgccgaagg tcaaggcggc acccaaggcc
     2941 gctgtcaata agctgtgttt tacgttcatg attatcatcc tttctgtcat tatgtttatt
     3001 atagcataat tcatgcggat aaaaccgtct ccggcagcgc cttatccata tcaaaactgc
     3061 cggcaaaaaa agaagtctcc catattcatg agagacttct gtatcatcag acggtgtctg
     3121 tgaaaagttt attccatatc gtaatcctga agggctgcga cgtgatgata gccgtcgttc
     3181 ttacctttat tggcaaagac gcgcaggact tcgcgagccg tgtccagaga ggtcaggcag
     3241 ggaacggcta attcaacagc catgcggcgg aggttgaagc cgtcgcgttc cggatgtttg
     3301 ccataggtca gggtattgag gaccaaatca attttgccgt tgcggatcat ggtcgcgata
     3361 ttctgaccgc cttcatggan nnnnnnnnnt gaccgatacc catgacttcg cctgtcgatt
     3421 tcatttcagg tccgagggcg atttcagcca ggccgatctt gctgaaggag aataccggag
     3481 ccttggcagc aacgtgtttc ttcgccggca tgaggccact gtggatgccc atatccttca
     3541 aggtttcacc aagggcgatg cgggttgcga tttcaacgat tgggaagtgc gttaccttgc
     3601 tgaggaaggg gacggtacgg ctcgaacggg gattgacttc gatgatgtac aatttgcccc
     3661 gcgatacgac gaactgaatg ttgaccgagc ctttaacctg gagttccaga gaaatgcggg
     3721 tcgtatagtc gacgatctgg gcgatcatat cgtcgtcgag gtgctgcggc gggtagnnnn
     3781 nnnnnnttcc atgatgcccg gaatgtagac gtcagttccg tcggcgacgg cgtcgacttc
     3841 gacttcacgg cctaccatgt attcgtcgat gaggatcgga tggtccggcg aagcgatgac
     3901 ggcttcgcgg agatagcgtt ctaattcctt ttcattatag acgatttcca tagcccggcc
     3961 gccgaggacg taagacgggc ggacgatgag ggggaattcc aattccgaag cgaccttcat
     4021 ggcttcttta tcgctggtcg caatgcggcc gtccggacga ggaatattga gtttagccag
     4081 gacttcatca aagcgttccc ggtcttctgc ccggtccatg ccgtcgacag aggtgccgag
     4141 gacggtgacg ccgcgcttag ccaagggcgc tgccaagttg atggccgtct ggccgccgaa
     4201 ctggcagatg acgccgacgg gcttttcttt ttcgacgaca gccatgacgt cttcgaccat
     4261 gaggggttca aagtacaggc tgtccgacgt atcgaagtcg gtgctgaccg tttccgggtt
     4321 attgttgatg ataatgctgt tcatgccggc cttgcgcaga gcccaggcag aatggacgga
     4381 gcagtagtca aattcaatgc cctggccgat gcggatcggg ccggagccga ggacgatgac
     4441 gctcttcttg ccgaggggct tgacttcatc ttccgtcgca tagcaggaat agtagtacgg
     4501 cgtagccgct tcaaattcag cggcgcaggt gtcgaccatc ttaaacgtcg gtgtaatgcc
     4561 gtggtcgacg cggaactgac ggacttcttc ttccgtcttc ttcgtaaatc cggcgatgac
     4621 ggcatcgggc atgctgtagc gtttggccaa acgaacgttg tcgatggtca agttattgcg
     4681 ctggaggtcg aattccacat cgatgatgtt tttaatcttg tggaggaacc acaggtcgta
     4741 accggtgatg tagtggatct tttcttcact gataccgagg cgcagggcct gagcgacgac
     4801 aaagagccgt tcatcatcct gcttctgcag gagttcgacg atatcgatga gggacttgct
     4861 ttccagcttc ttcatggaaa tgtagttgac cccgatttcc aaggagcgga cggctttgag
     4921 cagggcccct tcaaagcagc ggtcaaggga catgacttca ccggttgcct tcatctgggt
     4981 gcctaaggat ttatcggcca aggcgaattt ttcaaagggc cagcgcggga acttgacgac
     5041 gcagtagtcg agagtcggtt cgaagcaggc cgtcgtctta cccgtaacgg cgttagtaat
     5101 ctgatccagg gttagacccg ttgcgacttt agcggcaact ttggcgatgg gatagcccgt
     5161 cgctttcgaa gccagggccg acgaacggct gacacgggga ttgacttcga tgacgtagta
     5221 ctgattgctg ttcgggtcga gggcatactg gacgttgcag ccgccttcga ttttcaagcg
     5281 gcggatgata tcaagggatg ccgtgcgcag catctgatac tgcaaatcgt tcaaggtctg
     5341 gctcggagcg acgacgatag agtcgcccgt atggatgccg acggggtcga cattttccat
     5401 gttgcagacg atgatgcagt tatcggcctt atcgcgcatg acttcgtatt cgatttcttt
     5461 ccaaccggca acggagcgtt cgacgaggat ctgattgatc gggctgagtt tgatgccgcg
     5521 ggtggcaatc atgaacagtt cgtcttcgtc gtgggcgatg ccgccgccgg taccgccgag
     5581 ggtaaaggcc ggacggacaa tgatcggata gccgatttta tcggcaaatt caatggccga
     5641 cggaacgtct tcgaagatat cactttccgg aacgggctgg ttgatatcgt tcatggcctt
     5701 tttaaataat tcacggtctt ccgcttcttc gatagcggac aggcgcgtac cgagaagctg
     5761 gacgttatat ttttcaagga cgccggcatt ggccaactga acggccatat tgaggccgac
     5821 ctgaccgccg agggtagcca agaggccgtc gggccgttct ttttcaatga cttccgtaac
     5881 gtagtcgagg gaaatcggtt cgacatagac gcggtcggca atgtcttcgt cagtcatgat
     5941 cgtcgccggg ttgctgttga tgaggacgac ttccaaccct tcttctttca aggcacggca
     6001 ggcctgggta ccggcatagt caaattcggc ggcctgcccg atgataatag ggccggaacc
     6061 gataacgagt actttcttca atccttctac catgttattt ttcctccatc atcttgacga
     6121 attgggtaaa cagatacaga ttgtccgtcg gtcccggcga ggcttccgga tggtactgga
     6181 cgcacatgat gttctgggac gggacttcaa aaccttcaag ggtgttgtca tggaggttgc
     6241 ggtgagtaat gacgacctta tctttcggaa gagattcttc gtcgataata tagccgtggt
     6301 tctgagacgt gatatagacc cggcccgtgc ggatatcctt gaccggatgg ttggaaccgc
     6361 gatgaccgaa agtcaatttg cggctcttgc cgccgagggc caaaccgatg agctgcannn
     6421 nnnnnnnaaa tcggtttctt gcccatgagc tgtttaaccg tatcgatgat atcgggaata
     6481 tccttcgggt cgccggggcc gttgctgagg aagatgccgt cgggattgat ggccaatacg
     6541 tcttcggcct tggtcgtggc cgggaagata gtcaggcggc agtcgttgtt gaccagttcg
     6601 cgcaggatat tgcccttggc gccgtaatcc ataacggcta cgtgatagcg gccgtcgccc
     6661 atatgcttga tgtcccgcgt tgaaactttt tttacgaact gcgtttcaag gggcgtatca
     6721 aagagttctt taatggcgct ttcttccatc gtttccggta cgatgacgcc cttcatgacc
     6781 ccctggctgc ggatagcccg ggtaatggca cgcgtatcaa cgccgtagag gcaggggatg
     6841 tggtgtgtca taataaaggt agacagctgg ccttcattct gccagttgct cggttcttca
     6901 caaagttcac tgacgatgaa gccggcagca taaggacgat cggcctgttc gatttcgtgg
     6961 aacaagccgt aattgccgat aatcggatag gtcagcgtaa tgatctgacc ggcataggac
     7021 gggtccgtaa aggtttcctg ataccctgtc atgccggtat tgaagacgac ttcgccgaca
     7081 gccggttcat ccatcagcat attgccgtaa aatttctgtc cgttctctaa tatgaggaca
     7141 cctttcatta taaaacctcc ccgtttttca tcacaaattt accattgacg atggcagctg
     7201 ccgccttccc tttgaaaatc atttcatcaa aaggagtcga ctttcccttg gaatagaatt
     7261 tcgaggaatc gactgtccat tctttatcga gatcaaggat cgttaaatcg gcatctttac
     7321 ccggtgcaat gacgccggca tcgaggccga gtattttggc ctgggccgtc gacatgacat
     7381 tgaccagact catgagatcg atgatccccg tatgatacaa atgcgtcagc atggcgccga
     7441 cactcgtttc aaggccgaca aagccgctgg gcgccaaatt gaattcctgg tccttttctt
     7501 cccattcatg aggcgcatgg tcggtaacga cagcatcgat agtgccgtct accaatcctt
     7561 cgacgcaggc atcgcggtgg atgtccgagc gaatgggagg attcattttg tagcgcgttt
     7621 caaaggtccg cagggcttca tcggtaaaga tgaggtgctg cggcgtgact tcagccgtga
     7681 ccttaacgcc ccgcttttta gcctggcgaa cgaggtcgac ggcgttcttg gtactgatgt
     7741 gggcgatgtg gaccggcgtc cccgtcgctt cagccaggaa gatatcgcgg gcaacggcga
     7801 tgtcttcggc aacggccgga cggcccttca tgcccagttc gttcgatacg acgccttcgt
     7861 gcatattgcc gccttcgatg aggttgtcat cttcgcagtg gtcgatgatg atcttatgga
     7921 acatgctggc atattccatg gctttacgca tgaagtcgga attttctacg taatgaccgt
     7981 catcggaaaa ggcgacgact ccttctttgg ccatagagcc catagctgag agttcctggc
     8041 cctgttggc
//
LOCUS       DAWDXZ010000016         8022 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3026_length_8022_cov_8.197565, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000016 DAWDXZ010000000
VERSION     DAWDXZ010000016.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8022)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3026_length_8022_cov_8.197565"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1078..1087
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5503..5512
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1989 a         2178 c         1936 g         1899 t
ORIGIN      
        1 gaacaattat cggtctgcgc cgaagctacg aaagaagacg tcgacgctgc cgtccgcgcc
       61 gccgaagctg ctttcccggc ttggaaaaaa gtatccccca tcgaccgtca gaatatcctc
      121 ttgaaaatcg ctgatgttat cgaccagaac gccgataaat tagctctcat cgaatccctc
      181 gacaacggca aaccgattcg tgaaacgaga aacatcgaca ttccttacag tgccgaccac
      241 ttccgttact tcgccgccgt cctccgcgac gaagaaggcc gcgtttcgag cctcgatgaa
      301 aacatgatga gtatcgtcat ccatgaaccg attggtgtcg tcggccaggt catcccttgg
      361 aacttcccct tcctcatggg tgcttggaaa ctggctccgg ccttagctgc cggctgcacg
      421 atcgtcctca aaccgtcgag ctcgacatct ctcagcctcc tcgaattgat gcacctctta
      481 gaaggcgtcc tgcctgccgg tgtcgtcaac atcattaccg gtaagggatc tcgctccggc
      541 cagtacctcc tcgaccatcc ggacatccag aaactggcct ttacgggttc gacggaaatc
      601 ggccgcgacg tctacaaagc agctcaggaa aaattgattc cggctaccct tgaactgggc
      661 ggcaaatcgg ccaacatctt ctttgaagac tgcaaatggg acatggctat ggaaggcgcc
      721 gaattcggca tcctcttcaa ccagggccag gtctgcagcg ccggttcccg tatcttcgta
      781 cagcgcagca tttacgacaa attcgtaaac gaactggctg aacgcttcaa ccgcgtcaaa
      841 gtcggcctgc cttggctgaa agaaacgcag atgggcgctc aggttgacga acgtcagctc
      901 gaaaagatct taagctacat cgaaatcggc aaaaaagaag gcgctcgcct ggtatgcggc
      961 ggtgaaaaga tcaccactga cggcctcgaa aagggcaact tcatccgtcc gaccatcttc
     1021 gctgacgcca agaacgacat gcgcatcgcc caggaagaaa tcttcgggcc cgtcgtcnnn
     1081 nnnnnnnttc gatacggaag aagaagtcat cgatatggcc aatgacagca tctacggtct
     1141 cggcggcgcc gtatggaccc gcgacatcaa ccgtgccctg cgcgtagcca aaggcgtaca
     1201 gacgggccgt atgtgggtca acacctacaa tgccctgccg gccggcgctc ctttcggcgg
     1261 ctacaaactc tccggtatcg gccgtgaaac ggatagatcg acgatgtacc actacatgca
     1321 gaccaagaac atcctcatca acttctcgga aaaaccgaac ggctattacg aataatactc
     1381 cgcccgctca tttcctatca taaattgaca aaaacctcct ttcttcatat aatacagtat
     1441 tgtgaagaaa ggaggttttt ccatgaaaaa gatgctgtcc aaaaaagaaa aggcccgtcg
     1501 ccaaagcatc cgcctgtcgc cggccatcaa aaaaacgatc cggacgctcg acccggcccg
     1561 tatcctgacc ctcgacttag agacgacagg acttacggca gacgccgaaa tcattcagct
     1621 ttccatcatg aacggtttcg gtcaggttct cttgaaccgc tatttcaggc ccatccatac
     1681 ggagcgatgg gacgaagcga tggaagtcaa tcacattacg ccggaacaag tggcccacga
     1741 agatcccttc atcctctggg ccgatgaagt cagccgtctt ttcatggacg cagacctcat
     1801 cgtcggctac ggtaccttta acgatatcga gcgcctctat gaatcgggcg tcgtcctgcc
     1861 cgaccgcgat atctacctcg atctggccga agccttttcc ctcatccatt ataaggaaac
     1921 gaagaccatt acctatacca agcttcgaaa ctgtgccgcc tattacggct atgacagccg
     1981 ccgcagctgg cacgacagcc tgaccgatac ggacgctacc ttgttttgtt tccagcacat
     2041 gctggaagat gaccgggcca tctttaaaaa acgccgttac ccgcagctct gcggataacg
     2101 gcgtttcgtc gttttgcaat tatactcctc gcaattgagg atcccaaatg attttgtgga
     2161 gctgaatctg aaggtgccag tcagtcagct cgtgtttctt cataaagtcg acaatctgcg
     2221 ccggctcaat ttctccgaag acggcaccga cgtagacggc acacttcggc tgatagcgtt
     2281 catagacggc cagggcctgg ttgaggtcgg cttcatcgcc gacgacgaac ttcaggacgt
     2341 cctgaggacg gagcttttca aaattctctt ccagcatccg gccggccatg tccgacgaga
     2401 ggctcttata gtcgacggta aaccagacat ggtcgtattt gaagtaaggc tcgatgtcga
     2461 cgctgccatt cgtttcgata ttgacgctgt gcccttttag ggccgccaac agagcatgga
     2521 cgtcctgaca gagcggttcc ccaccggtca gggtgacgtt ggggtacgag acctgcctta
     2581 caatgtcgtc gacgtccatg tccttccctt ccgaaaaggc ataggtcgta tcgcagtaag
     2641 aacaacgcag gttacagcca cagaggcgga tgaaggtggc cagctgaccg gccttcttcc
     2701 cttccccgtc gatgctgtcg aaaatctcat tgacgcagag actatacttc atagacagcc
     2761 acgttccctt cgctttcctg gacctcggcc cggacgcaat gcggtacagt gtcacaaatc
     2821 cacttggcaa tgttttctgc cgtcggattg aaatcaaaga cgtcgtttaa attatgatga
     2881 tccatcttgg agtggaccag ttctttaatc tttttgaagt cgacgaccat accgttgtga
     2941 tcgagttctt cagcctgaca ggtcacatgg atgatccagt tgtggccatg gagattttca
     3001 catttactgg cgtagtcgag gactaaatgg tgagatgcgg agacttccag ccgcttggtt
     3061 acagtataca aacaatttcc tcccttatcg tgcatcaaaa ggcttataag cccagcttgt
     3121 agatgcggtt ggcgttatta aagaatacgt catcccagta agcttcaggg atgaaacctt
     3181 ttacaaatga aatgtagtcg ttaacattgg ccaagggcca gtccgtccca tacatgaccc
     3241 ggtcataggc gccgagatac ttgagccaat cccggagcat ggagatataa ccccgcttac
     3301 tgcgcaggaa ggccatcata tcgggcacct tgccttcgag gagtcccgac agatcgacgg
     3361 cgacgttgtg gttcttttcc aagacggcaa tggcatcctg caaaaaggga ttgccgaagt
     3421 ggcacatgac caactggacg ttgggatggc gcacggccgc ttcgtctagg gtcaggggat
     3481 ggctgtattt caacagggcg ttggccgttg ccgtcagccc ggtgtggatg gcgacgggct
     3541 tatcatactt ttcggccaat tcataaatcg ggtcatacca ttcgtcgtaa acgtagatat
     3601 ggctgtagcc cggatagagc ttgatgccgc agcactgact tttctttaaa ttctcttcga
     3661 cattggcggc aatgctgtcc atgtcatctt ttccggtcag gctgtcgatg ccgatgcagt
     3721 agctcatata atcgggatag ttatgttttt tagcatccag ggtccgatta ctcatgacga
     3781 cgccgtggac gacgttattt tcgataaaag cttggtgcag tgattcttcc gtattggtat
     3841 agccggcagc cagggcgacc tggtcgaagc cggggaaatc gcaaaaatgg aaatgactgt
     3901 caatcactcg taattctttt actttcattt ataggtccct cattccaaaa tcgccgtttc
     3961 taatgcaatc tggatcatat catcaaaaga cgtctgccgt tcttccggcg tgcaggcgtc
     4021 tccggtcaat aagtggtcgc tgacggtaaa caaccccagt gccttggcgc caaacttggc
     4081 cgctgccgta tagagaccgg ccgtttccat ttcgacggcc atgatgccgt actgggtcag
     4141 tttttcgtta tcgatttctt catcgtaaaa acggtcgaca gagacgacgt tgccgacccg
     4201 ggccgggacg ccaagagccg ctgcctgacc ataagcctgg cacaaaaggg taaagtcggc
     4261 taaaggcgca tagttaatcg agccgccgaa aatattgcga atgatcgacg aatcggtcgt
     4321 cgccccttgg gccatgatga cgtcgcgaag tttgatatca cggcgcatag caccgcaggt
     4381 accgacgcgg atcagctttt tcacaccgta gtcccgcatc aattccgtag cataaatgga
     4441 aatagaagga atccccatgc ctgtcccttg gaccgacagg cggacgccct tataggttcc
     4501 ggtatagccg aggatattgc ggattcgggt atagcagaag gtgtcatcta aaaaattttc
     4561 agcaatatat ttcgcacgca gcggatcgcc tggaagcaag atgcgatcgg cgatttcacc
     4621 gtcgcgggcg gcaatatgaa gactcatgta cagttccccc tttacacatc atctattgaa
     4681 ttcctttcat aacatgcctg aaagggcggt tatggagtga aaaacatgga agtccatgct
     4741 tgattttatc aatcctcaag tctatatgat accacattac tctagtttta gtcacatcat
     4801 tttcgctatt tttcatattt ttttcagaca aagcctattt gacaagacct agggaatttc
     4861 atataataaa gtaattaagg atgccccatt ttgactgccc ttattgcggt acttggcggg
     4921 catccttttg tcgtattgca gtttgaaatt gcctctcgct atgctacaat gtataagaag
     4981 cattaaaagg agcggtcgta tgtacgaaaa tcccttatct cacattttag aatataattt
     5041 agacttgggt aagacgaagc ccgatacggt ccatcgcgac gaaaggtatt gccccttctg
     5101 tgacgtcgct catttgaaga atgtcatgga aaagcggggc cacatgatct ggcttgaaaa
     5161 tgcctttccg gtcctcaagg actcatggca gacactcatc atcgaaaccg atgactgtga
     5221 cggcgagttc tccgactata ccccggaata cgcccaaaag ctcatcgaat tcggccttga
     5281 aaagtggcag caagtcaagg aaatgggcaa attcaaatcg gtcgtctttt accgcaacca
     5341 cggctatatg tccggcggca cgattcacca tccccattcg caaatcgtcg gcttcaaaga
     5401 ctatgactat cacgaagata tcaaaccctt ccacttcgtc ggtccgacaa tctttgaaga
     5461 agacggcctt tcgatccccc tgtccacacg gcccatcatc ggnnnnnnnn nntcatgcac
     5521 tccttcatcc gctttaacga cagttacaac atctttttct atgattttcc cgatgacgat
     5581 gccttatacg tcaaaatcat cccgcgcttt cttaccaacc ctctctttgt cggttacatg
     5641 attccacaaa tcgcaaatgg aggccattcg gcgcatttta tccaggaatt gcagaccagg
     5701 ctgcatgacc tatagaaagg acggtcctca tacatttatg cgtttatttg caataggtga
     5761 cttacattta tcgggcaatc cgccaaaaaa gccgatggaa gtcttcggcc cccgctggaa
     5821 aaatcactgg cagcgcattg ccgaagactg gaaagaaaga gtcaccgatg acgatacagt
     5881 cctcattgcc ggcgatacgt cgtgggccat gcgcctcgac gaagcccagg aggacctcga
     5941 tcaaatccgc gcccttccgg gcaaaaaaat cattatccgc ggcaaccacg actactggtg
     6001 ggccagtgcc ggaaaactga accgcatgga tgccgaaaat aatatgcact acctctacgg
     6061 ctcgacagcc atcctcgacg atggcacgat catctgcggc acccacggct ggatctgccc
     6121 gggcgacatc cattaccagg aagaaaggga tgccaaaccg taccgccgtg aactgcttcg
     6181 cgtcgaacgg acgctccagg aagcggaaaa aataggcagt ggccatacca ttctcatgct
     6241 ccactatccg ccggtctgcg acttgacgaa acccagcggt tttacagaac tcttagaaaa
     6301 atacaaggtg cccctctgta tcttcggcca ccttcacggt atgcagcccg gaacgatgtt
     6361 cccccgccgt tataacggta ccctgctgca cctggtgtcg gccgactatc gggactgcaa
     6421 gctccttgaa atcaaatttg acggtgagga ggcactgcca tgagtcaaat agcccaaaaa
     6481 catctcttag ccggcatcat cttcggcgac gccgaaacgg gcgaatacat ctaccttccc
     6541 ggtggcgaag tcggtaccga ccgaccgctc tgcgtctttg aacacgacgg tcaaaaagag
     6601 gatgtcgacc tcgaacaagc cggctacctc gtcgatcacc tgacgctgaa aaaatgcagc
     6661 catcccaccc tcgggaaccg ctctttttaa cataacaaaa aagaacctct ctgccctcct
     6721 gccgaactca ttcgttagga tctaacgaat gagctgcaca gaatcagagg ggttctttta
     6781 ttatgcgtta tccggcgttt gatcggcatc ttttcggagg gtaaaccaga aagtgacgcc
     6841 gccgttttcg ttcttcacgc cataagattc accgtggagc ttcaccaggg ccgccaccgt
     6901 gcccaagcct aagccgtggc cgccgaagat gcgtttcgga ctgcgggccc gcgacgagtc
     6961 gatcttatag aagggctccc aaatacgctg ctgatactct tcatcaatgt gactgccttc
     7021 attgtagacg cagattcggt accgctcacc gagatcttcg gcccaaaggc ggatatgttg
     7081 attggcatcg gtataatcga tagcattgct taagaaattc gttaaaatcg tatcgattcg
     7141 ttccggatcg ccatagacgg tcatggtctc aggcaaatca atcttggcaa ggattccctt
     7201 gtcttccatg actttggaga accgttcccg actttcctcg gccagagcac tgaaatcgaa
     7261 gacggtctgt tccagatgga aggaacccgt ttccaaacgt gataagctga gcagatcacg
     7321 aaccagttta tccatcttct gggtctcact atgtataacc gagcaatacc gacgctgtgt
     7381 ttcttcatcg atgtcgaggg tatcgagacc ttctgcgtac ccttggataa tggccaaggg
     7441 cgttttcagc tcatgggaca cggccgatag gaaggccttc cgcatgtgct cgacctcttt
     7501 ggctacgtcc aattgttttt tcagctcttc attcgattgg cgaagagccg tcaaggcgac
     7561 attgagctgg ttggacagga tattcaggct ctcaccgagt tcgccgattt catcgctaag
     7621 gttccctttc cacttattgg aaaaatcaag gtgagccatg gcgaaggcca ggttcttcaa
     7681 ttcgatgagg ggtttagtca tgcgctgagt caggtacaac gtactgacgc aggccacgat
     7741 cagccagacg atgccgcaga cgatgatgaa atgctggaca atatagatat tttcctgcat
     7801 gggcgcgacg gactggttga tgatgacgta cgtaccatcc tgaatgcggt cgacgaggtt
     7861 aaaatactca aaatcaggca tatccttata aagatgaacg gctcccaaca ggctttcatc
     7921 aggtgccttg ccgtcgagaa gttccatgag ttccttgatg tgtaccggcg gcttgccctc
     7981 cggacgatcg gcaggaactt cattttcctt gccaagggcg at
//
LOCUS       DAWDXZ010000017         7874 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3061_length_7874_cov_9.201305, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000017 DAWDXZ010000000
VERSION     DAWDXZ010000017.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7874)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7874)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7874
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3061_length_7874_cov_9.201305"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    605..614
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2232..2241
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4406..4415
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6140..6149
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1794 a         2076 c         2034 g         1930 t
ORIGIN      
        1 ttccctgtaa tacgtgaata tcatattaat cgattttttt taaaatgtaa aaagacgacc
       61 gcccttttac aatgagacta ccgtcggcat cgcggcattc acattcgacg acaatcgtat
      121 ttcggccgtg atggagaacc tgtccgattc cggtaatgtg ctcgccgctg ttgacactgc
      181 gaatgtagtt catattgatt tccaaggtcg taacctgaaa acccatcgtc cggcaggcca
      241 tccccatgag ggtatcacaa aaggctgcca ggacaccgcc tgaggcaata ttacgataat
      301 tacaaatgtg gcggctaacg tcaaagccga cgcggacgcg gccgacagct accgtttcaa
      361 tggtgcaggg catgctcttg aaatacggcg ttttggcata aatgccttcg atgatggcat
      421 gcattctggc agagtcggac aggccgacgc cagttcttgg ctcatccatg gtccgtacct
      481 ccttgctctt cgccgccgta cgggccggtc acgaagaagg ttcccttccc atgcgctaag
      541 tagcgatcgt catcatcgta aaaatcacaa agagcgacca tcgtcgtccg gccgttgtga
      601 atgannnnnn nnnntcggca cggatgactg tatcggcaaa ggccgggcga agatagttca
      661 tgcccagatc caaggtcagg accgccttgc cgtggacagc acaggcagct tccatcgccg
      721 agtcggccat ggagtacaac acaccgccgt acatcgtacc gttgggatta tcccattttg
      781 agcccttatt ctcaaaggtg agaacagtac actcctgttc agccttggct acatggaact
      841 gcataaaggt atcaaaagga tttctttgaa tctcatgctc taaccattcg cgatccatta
      901 tgactccact ccttaggcta atacataaat cccttatatt ttactatacg aataatcatc
      961 cgtcaaacaa aaaacaacga tttgtgcccc ttaaactgaa ctttaagaat gggaaaaaat
     1021 aaggtcccgc atagcggcac gagcctttat gcaagacctt atcactgtga catgcctgac
     1081 ctggtcttta cccccacact cgcctgccgg gacccttgct catagactgt tgtcccccac
     1141 taatagtcct ctcgcgtttt atgacgcggc aggtcttact tctaggccgc actatgatca
     1201 gtaccgcttt gggcacctta cgtggatcgc ttcgtctgcg actgacatcg actttcaacc
     1261 acagcacatg tcattgatac attatacggg acaggctgac aaattgtcaa cgttagaact
     1321 tatagcccat accgacctgc cagccgcgga cgtcgtcatc gtagttgtcg acatcgacgc
     1381 tgctgcggcg atatttgagg tgagcgtccc attccggcgt aatgtcgtag ccgacgccga
     1441 tttcataggt attgacgtca tcgccgacac cgacagaagc aaagccctgc caacgttcag
     1501 aaataggaac ctggccgcgg acaccgacct ggtagccgta ggattttttc gtgttgtacg
     1561 tatcggtctt aacgtaagaa gcaccggcat aggccgaaag gtattccgtc aggcggtaat
     1621 tgccgacgag ataattttcg ttgatcttgt cggaatcgtc acccttcgta tagttgttga
     1681 tgtactggat attccaacga tcgttcagga cgtaggtgac gtcgccgccg gtaaagcggc
     1741 tgtcggcatc gccgtggcct tctttttggt caaatccata gttgatgttg gctttcaaat
     1801 caccgggctg ctcgtttttg ataggggctg cccacgctgc tgacgtaatg gcaaatgctg
     1861 ccaatgtcgc cgttaaaata aattttttca tgacatgaca cctccaaata gaattccttt
     1921 aaagtacatt tatgatcttc cataatagcg gctcaggagt tcttcgcctg cggcgatgac
     1981 ctggctcatg ggaatcaccg aaataccttt atagttacct tcgcggataa ttttcttcat
     2041 gctcttgccg acttcataat ggtccgtcga ttcgaggacg atggcccggt ccgtataggg
     2101 accgtagagc ttgggattgc tcggcccgta gagggcaacc aggggcaccc gttggctgac
     2161 ggcgatgtgc atggggccgc tgtcattagt gatgaacagg gagcagcggc ggatggccga
     2221 ggccaattca cnnnnnnnnn ntcaccgatg gtaaactgac cggtagcgac gataggtttc
     2281 gaagccatct cccccgtcgc ttccttcacc atatcgagat ccatgtcgcc tccgaagaag
     2341 acgcacttga aaccccggcg gccaaagtaa tcagctacag cggcaaatcg cttggccggc
     2401 caccgcttct gaggaacggc actgccgatg ttaaagccga tgaggctgtc tgccggcgag
     2461 acgccgtggg attcataaaa ggcggcggcc ttgtcatccc aggcctttga agtcgggaac
     2521 tgcagcccat cgcttcgata atcgtcgatt ccgagctggg ccaggacgtt gatgtacata
     2581 tcggcggcgt ggagatggat gcggtccagc cgcgtatagc ggttcatcaa agggcgggcc
     2641 aggaaatggc tcatgccgac gaatttctta gcatggatga cggttgccaa aaaggacgtc
     2701 cgttcattgg gatgcaggtt gatcaaaata tcatagtgct gttgatgcag gcggcggccg
     2761 atatgccaca aggcaccgat tgaattgtcc ttccccttct tatcgacagt gatgacatgg
     2821 tcgatatcgg ggttataacg gacgacgtcg gcaacttttt cgtcgacgac gagggtgata
     2881 tcagacccct gggcgtggcg gcgcaggacc tgcagaaagg gcgtgatgag aatgagatcg
     2941 ccaagatgca tgagaaaggt gacaattatt tttttcttgc gtaaattaat catggttcca
     3001 ccttcttcag tacctcttca aaaaggttcc acaccgtatc gacagtaatc cgggccatgc
     3061 agtccggatc gtcgacgaga ggttcttccg gcgtggcttt cgacgtcggc gaaatgacga
     3121 gatggatata agcgctgtcc tgtcctccat aaggtccggt ccgttcagga gacgtagtgc
     3181 caaaaagggc gatgagctcc tttttgaggg cattggctat gtgaagggga cccgtgtcgg
     3241 cgctgagata aaccaggctg tcatcgatgg ccgccatgag ctggcgaaga tcggtcttgc
     3301 cggtatagtc aaaaaccagt cccgacgcat cccgttcttt aatccgacgt cccttctccc
     3361 gatcgtcatt gccgcctaag atgacagctg acaggccctt atcggtcagg cgttttaaaa
     3421 agcgactcca atggtcaaga ggccattctt taacattcca gcgagcaccg ggaacgacgg
     3481 cgatataagg ccctttgagg ccgtcttgca aaaggcgctg acgccaggcc tccttctctg
     3541 ccgtaatatc cggcaaggga aaaaccaagt cgtcgacacg gccgcctaag gcccgaatcg
     3601 tatcgaggta gcgctcgatg acgtggtcgt atttatgagc cccgaccagg cctttgctga
     3661 ctaaaaagct tccttcgcgc atttcccaat agccgtattt ctctttggct ccgctgagga
     3721 aggcaacgat ggcgcttttg gccaaaccct gcaaatcgag gcagaggtcg aaatgatagg
     3781 gatggagtgt cttgcgcagt tccatccagt atgacaagga tttcagccgt tttttatcga
     3841 catagatgac gtcatcaatg tagggctttc ccggcagcac cgaggcaaac tgccgatgaa
     3901 cggcccagac gatgcgggcg tcgggacaat tttgtcgcag ggcatggagg gtcggcagcg
     3961 aatgaagaac gtcaccaaga gaactcatct tgatgaccaa tatgttttta tacgctttca
     4021 cgttccttcc tttctgtgac aatagccttt acaaaggcat cgagcctgcc gccggtgaat
     4081 aagtagccgt ccatgccggc tcgccggccg cattcgacgt cacggggcat gtcaccgatc
     4141 ataaaggatc gggacaggtc gatatcgtag tcgcgggcag ctgccaggac catgcccggt
     4201 tccggtttgc gccaatcgct ctttttatca tacgctttta tcggcgcccc ttcgaggtaa
     4261 gggcagtaat agacggcagt aatcgtcccg ccggcagcgg cggcttcgcg gcacatccaa
     4321 tcgtggaggg tctttacatc gtcttcggtg taataaccgc gggcgacacc gctctgattg
     4381 gtgacgacga tgatggcata gccttnnnnn nnnnntcggc cgtcaagtag gccagggctt
     4441 ctttggcgcc gtccatccat tcaaaatcct tgatgaatcc gaggtacgat ttatcgacgt
     4501 tcaagacgcc gtcgcggtcg aaaaagacag cctttctcat gcgaaatagc cgccgttctt
     4561 ttcgaggatg ctgcagtatt ccttaacggc ttccgacagg atggtaaagc ccttgtcata
     4621 gccggctttc atgagatttt ccgtatcggc ctgggtgtag ctttgatatt tgcccttgag
     4681 aatatccggg aagggaacgt attcgatatg gcccttgccg caataatcga tgacgccttg
     4741 gacaaattca ttgaaggtat ggccgatacc ggtaccgcag ttatagatgc ccgacagttc
     4801 cggatgttcc cagaaataga agatgacgtt gaccacatcg tttacgtaga tgaagtcgcg
     4861 gatctgaccg ccgtcttcat agcctgccgt ccccttaaag agggtgattt tccctgtttc
     4921 ttggagttca tggtatttct ggaataccag ggatgccatc ttgtccttgt ggttttcctg
     4981 cgggccaaag acgttgaaat aacggaaacc gacgatctgg ctcttggcga taggctgaac
     5041 gcggcgaacg aaacggtcga actggagctt ggaatacgca tagggattga gggcctcttc
     5101 gcacgacgga tcttcacgga agccgtttcg gccgctgccg taggtcgacg ccgacgacgc
     5161 gtagaggaag ggaatcttcc ggctcatgca gtagcgcaaa agattcttgc tgccttcata
     5221 gttgttcttc atcatgtagc ggccgtcgta attcatcgta tccgagcagg ccccttcgtg
     5281 gaagacgacg tcaatgtagc cgcagtcaaa ggtatcatcg gccatctgtt catcgaagtc
     5341 ggcgtaatcc atataatcaa agaaatcgag gccctggagg ttgcggcact tgcggccgtc
     5401 ggtcaggtcg tcgacgatga tgatgtcgtc gataccccga ttattcagtt gctttacgat
     5461 attgctgccg ataaaaccgg caccgcctgt tactattttc atattttccc cttctttacc
     5521 aaatcagcta ttttctgaac gagtcccgtc gtcgaatacc cctcttcaaa agaaatgatt
     5581 tctacgcgct tgacggactc gcggccaatg acttcatcgg ccttataatc gccgcctttg
     5641 accaggatat cgggacggag gacctctaag agttccttcg gcgtgtcttc gtcaaagatg
     5701 acgacgtcat cgatacatcc caaggctccg agcagaacgg cccggtcttc ctgagctacg
     5761 agaggacgcg tttccccttt gaggcgccgt acggagtcat cagaattgag gccgacaacg
     5821 aggtgttgtc ccagcatggc tgcctgctgc agataaagga cgtggccgcg atggagaatg
     5881 tcgaagcagc cgttcgtaaa gaccacggtt tcccctttct gctgccaatc ccggattttc
     5941 ttggccatct catcccaggt caaggactga taaggctgcc accggacggg atgccaatct
     6001 tcccataatt tcaggagctc atggcgatga atgggatagg tccctacctt ggtgacgacg
     6061 ataccggctg cggcgttggc gatttgcagg ctggtgcgaa tggacaactt accggcaacg
     6121 gctgctaaaa aggcagcggn nnnnnnnnng cggctacggt atctccggcg ccggatacgt
     6181 caaagacttc ctgggcagtg gccggattgt tccagacctt gccgtctttt ccgacgacgg
     6241 tgattccctt ttcagaccgg gtcaccatga ggtgggcaaa ggaatactgt ttatacagct
     6301 catgggctgc tttgacgaca gcctggcctt cattggcaac aggggtcccc aagcagtcac
     6361 ctaattcttt catattcggc gtcacgaaat cggcaccgtt atacttgctc caatcgctgc
     6421 ccttgggatc gaccaagacc gggatatgag cttcattggc ggcttcgatg acgcttttag
     6481 acaaggaatc ggtaagaacg cctttttggt agtccgacag aataatccca tcaaggcctg
     6541 atgccagctg ctgtttcagc caggcgagaa gtgtttcctc ttccggttca gctaaaggcg
     6601 tcacttcttc gaaatcgaga cgaaccatct gctgccgaga gccgagaata cgcatcttgg
     6661 tcgtcgtcgc atggcctttt cgcacgacaa ggcctgagcc gtcgatgtgg gactcggtca
     6721 ataactcgtg gagcaagtcg gcgttttcat cgtcgccgat gagaccgccc aataagacct
     6781 ggcagtccaa gttagccaag ttggatgcga cattggccgc cccgcccaag acggacgtca
     6841 tgtgatcgac tttattgacc ggcaccggtg cttccggtga aatgcgttcg accttgccaa
     6901 aaacatagcg gtcgaccatg atatctccga taacggcaat tttcaggttc ggcaagacat
     6961 gataaaaaaa gtggtcgatg taattttctg tcatagcgtt cctcgattca atctcttatt
     7021 tttgttcttt cgtacccatg agatacttca attcagccat aaaactttct aagtcggcaa
     7081 actcgcggta tacggaagca aagcggatat aggcaacggc atcgacagag cgaagttcgc
     7141 gcaaaaccat ttcccctatt ttttcactgg acacttcctg agccagtgtg ttgcggatat
     7201 tgcgttcaac cttggtgaca atctcttcca gctgccggcg cgtgacttcc cgcttgttgc
     7261 aggcccgaag caggccgttg atcaacttat tgcggtcaaa cagttcgtgc cgtccgtctt
     7321 tcttgacgac gatgagcggt acttcttcca ccatctcata cgtcgtaaac cggcggccgc
     7381 actgctggca ttcgcggcgg cggcggatag ctttgccatc atcggttgcc cgtgaatcgg
     7441 ttacttttgt atctgcacat tggcaaaatg ggcatctcat ccttcttcct accttctttc
     7501 cgacgatatt ttcaaaaaaa gcgggtccgc cgggatccgc tttttttcat gctgattatc
     7561 tcatccacgc aggaatttct acgccgccgg cactatcttt tttatcgccg gattttgtcg
     7621 ttccgcccgt tccctgtccg aaagaaggaa tcgaagacgg ttctttaccg aaacccgtag
     7681 cgacgaccgt aatgcggatc gaatcttcca tgtccggatc gatgaccgaa ccgaagatga
     7741 tgttggcttc cggatcaacg gcttcagcca ctttttcagc agcttcgttg atttcgtaca
     7801 agctgacgtc cggaccgccg gtgacgttga gcaggatgct ctgagcgccg tcgatgctca
     7861 tttctaagag ggca
//
LOCUS       DAWDXZ010000018         7793 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3086_length_7793_cov_10.082321, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000018 DAWDXZ010000000
VERSION     DAWDXZ010000018.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7793)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7793)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7793
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3086_length_7793_cov_10.082321"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2591..2600
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4379..4388
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1890 a         2146 c         1865 g         1872 t
ORIGIN      
        1 aaatcgcata ttggagtgga acaggtaata cggaagcaat ggcaagtgaa attgaagcag
       61 ctgtcaaagc tgccggtgca gatgtagaat cggttcgctt cgaagatacc accgtcgatg
      121 atgtagccgg taaagacgtt attttgctcg gctgcccggc catgggtgct gaagaattgg
      181 aagactccat tgtcgaacca ttctttgcag acctcgctcc caaattaaaa ggcaagaaag
      241 tcggcctctt cggctcttac ggctggggct ccggcgaatg gatggataat tggaaacagc
      301 gtacggaaga cgctggcgcc accatcatcg ggacggctat cgtcaacgaa acgccggata
      361 atgctcccga gtgcaaagaa ttgggcgaac ttgccgctaa ggcataagtt gttttaactt
      421 catattcatt ctcctctagg aaaggggctg catacccgac acagtatgcg gtcccttttc
      481 tcatgtctta gctgtttttt tcgattggga acgtttttga tcccaatttt atacgttttt
      541 gtcaagtgcc catgcgatat cgatacagtc attctctact ttcatggtac gcattcccgc
      601 tggctatgct acaataataa cggattacat tcattctcat tttttatttt taaaggaggc
      661 tttattgcat gagagaccag gtcttttcta ttttacaggc tgtcggtcgc agcttcatgc
      721 tgcctgtagc catactacct atcgccggtc tgcttctcgg tatcggcgcg tctttcacaa
      781 acgccacaac catccagacc tatggattgg aaaacattct cgggaatggg accatccttc
      841 attccctgct caccatcatg agtgatgccg gcaacatcat cttcggcaac ttgccgatca
      901 tctttgccgt cggcgtcgcc atcggcatgg ccaaacaaga aaaggaagtc gcagccttgt
      961 cggctatgat tgcctttttc gtcatgcacg catcgacgag ttccgtctta aaattgacgg
     1021 gcaaggtact cgccgacaat accgtttccc cggacgtcct cgaaggtacc attgccaaca
     1081 tcaacggtat catgtccctt caagaaggcg ttttcggcgg catcatcgtc ggtatcggcg
     1141 tcgcctacct gcacaaccgc tttcataaga ttgtcctgcc cgatgcctta tcgttcttcg
     1201 gcggctcccg tttcgttcca atcatctcga ccgtcgtcta catgttcgtc ggcattgcca
     1261 tgtacttcat ctggcctgtc gtccagaacg gcatctttgc cctcggcggt ttggtaaccg
     1321 gcacgggata tgtcgggacc ctcatcttcg gtatcatcaa acgtgccctc attccgttcg
     1381 gcctgcacca tgtcttctac ctgcccttct ggcagaccgg cgtcggcggc tccatgatga
     1441 tcgacggcaa gctcatccag ggcggccaga acatcttctt tgctcagctg gcctcgccga
     1501 acgtcgctca tttcagtgcc gatgcaacgc gctacttctc cggtgaattc atcttcatga
     1561 ttttcggtct ccccggcgca gcccttgcca tgtaccgcac ggcaaaaccg gaaaagaaaa
     1621 aggtcgccgg cggcttgctc ttatccgcag ccttgacgtc catgttcacc ggtattaccg
     1681 aaccgattga attctccttc ctcttcgtcg caccgatgct gttctacgtc caggtcatcc
     1741 tggccggcgc agcctacatg attgcccata tcctgaacat cgccgtaggc cttaccttct
     1801 cgggcggtct cctcgacctc ttcatcttcg gcattctgca aggccaagac aagacgagct
     1861 ggatgtacat catccccgtc ggcatcatct acttcttcct gtattacttc atcttcacct
     1921 tcctcatcaa gcgctttaac ttaaagacac cgggccgtga agacgatgat acggcaacga
     1981 agctctacac caaagccgat gtcaaggccc gcgatgccgc agccaaagaa agctcagctg
     2041 acaaggcttc cggcgatgcc gtcagccaaa cgattactca ggccctcggc ggcagccaaa
     2101 acatcacctc tgtcgatgcc tgcgccaccc gcctgcgctg cactgttgct gattccgctc
     2161 tggtaaacga cgcagccctg aaagcaaccg gtgccgtcgg cgtcatcaag agaggtaccg
     2221 gcgttcaggt cgtctatggt cctcaggttg ccgtcatcaa atccaatctg gaaaattacc
     2281 tggcaaccaa tccccaggac ggccccatcg aagccgcccc ggaagccgcc ccggaagcgg
     2341 caccggttga agagccgaaa aaagaaacag ctccagaaaa atcagacgct gaaaaacgct
     2401 acttcaactc gccattcaca ggagccctcc atccgattac ggaagctccg gatgaagcct
     2461 ttgccggaaa aatgaccggc gacggattct tcgtatatcc ggaagatggc gtagtctatg
     2521 caccggagga cagtaccgtc accttcgtct tcgatacgaa acatgccctc ggcatgacca
     2581 ccgccgacgg nnnnnnnnnn aagataaaaa aatgagatgc cccttcggga gcatctcatt
     2641 ttttataccc ttaatagaga cttttataga tttcgtaaac gtcgtcataa gacaagggaa
     2701 caaagttatg ggccatcaat cggccctggt tttccatgac cgacttggca aattcagcca
     2761 gttccgcttc cttcaccccg tattcatgaa gggctttctt cggcaggatg acgttaagca
     2821 gtttttccag ctcatcatag acgccatcgg catcgcagcc taagatagcc gcaataaagg
     2881 cgttgagccg ggcaatttcc ccatccgatt taatcgtcat gtaatgcttc atgacgccgg
     2941 taaacatggc gtaattcgat tcaccgtggg gcacgtggta gttcgctccc aagggataac
     3001 tcatagcgtg aacggcagca caaccgggaa cgctgaaggc gatgccggcc caggtgctgg
     3061 ccaaaaggaa atctttcatc aagggaaggc gggcttccgg accgtggtcg cggatctgta
     3121 aataaccgct gaggattcgt tcaatggcct gatagctgaa gacgcggaaa ctggcgctgg
     3181 aatcaggcga caaggccgat tcgacagcat ggacgagagc atcgatagaa ctcgtcgcaa
     3241 agacgggaaa gggcagggtc ttcaataact gggaaatgag gacggcctta tcggcataaa
     3301 gggcgttact cgcaatcccc tttttcgtgt ggcggctgat gagggccatg accgaaacgt
     3361 tggtcacttc actgcctgtg ccgcacgtcg tcggaaccaa gatcagttcc cttccttttt
     3421 caatggccgt cttcccgtca aagaggtcga gaatcgggct gaccttcttc aaaacgaata
     3481 acttacaaag gtccatgacc gtgccgccgc cgatgccgat aattcgtttc acatcagccg
     3541 gtatatctcg gtaaatggct tcggccatct cgtcagacgg ctcgccgctg ccgtattttt
     3601 cctgaaaaag aacgtggcag ggaagattca agactttaaa attcggttca aaaatatact
     3661 cattggtaat gatcaggtcc cctgctccca ggtgaaaatc ggaagcaaag tcccggcacg
     3721 tatcataaga gtaaatcgtc ggtttaagct gtaattcaat cattcgaaca gactcctttc
     3781 aaaagcagta tggctattat ctacagtgta caaaaatgca aagcgttttt caaccgtatc
     3841 ggctccatgc catccgtgat ataatatacc tattatggga aaggacgggt tctggtgttc
     3901 cctctggtct tcaaaaccag cgtgaggggt taacagcttc tcaggtgggt tcgatccccc
     3961 ttccccgcca taataaagag attgcgaatc ccttgttgaa aagcatccat ccggacaagc
     4021 ccctaagaac aagatagaag acaacaaaca gcccttcccg attcggaaag ggcttattct
     4081 tttaaggaag aaccgctata tcgacggtca aaatttttga ccggccttat cttttttcag
     4141 ccactcaatg aggacatctt cacgccgtct ctcgatgttc tccagttctt caggcgtcca
     4201 gggataaaga ccggtccccg ttttagcacc gaggcggcct tcatcgacgc actgtttcag
     4261 gagagccgcg ccttccttat cgtcggccaa gtcggcattc agataggcgg aaatattgta
     4321 gaagacgtcg agaccgccaa gatcggccgt ttcaagaggc cccgtaacgc cgagccgcnn
     4381 nnnnnnnnca atcccgtcct gaacacaatg aatggcctcg cgaaggaggg caaactgcag
     4441 gcggttaccg acaaagcctg gtgcttcttt taagagcgcc gccggctttt tcccgatttt
     4501 ttccatgagc ttccacgtaa tatccaccgt ttctcggctc gtcttttcac cgggaacgac
     4561 ttccaccaag ggcatgaggt gcggcgggtt ccaaaaatgg gcgacgacaa accgttccgg
     4621 atggtccaag acggacgaca cttccgtcgg cgagaatccc gatgaatcgg tcgcaaaaat
     4681 gacgtccggt ggaagatatc cttctaattc ccggtacacc tgctgtttaa cgaccatatc
     4741 ttcagccacc gattcaatga caaaatcggc atcacgggcg gcttcttcaa tcgtcgtaac
     4801 gccgtgaatc cgcttcacca aggccctttc ctcttcatca gtcagcagtt cattagcctt
     4861 cataatggcc agcgtcgccc gaatattccc catagcccgt tcaatactgg catccttgcg
     4921 gccaaacatg tttacgtcat agccatgacg ggcaaagtca atggccgtgc caaaacccat
     4981 ggtgccgcag cctaaattgc aaatccgttt aatctcgttt acttgtttca tatcgtatct
     5041 cccgttcgct gtgatatgcc ggtgaatggg ccaagaccgc gcaaaaccgg gtaagccacc
     5101 atcttaccgg ccttatagta tatataataa aggaccggcc tgtaaatctc aatataaaga
     5161 tgagtgaaca ccgcctaaag catagctgca ctacacaaag acagcgtaat catctgcacc
     5221 gaaggctgcc gccccccctt tatagaaata gaagcctcca aatcatggta aaagggcggt
     5281 ttctccaaga ttcgaaggct tttatttcta tgagcaggtt tttcataact cgtgcttttc
     5341 cgtcagttac cagcttgtcg tgctggttgg taacggtagt ccggaataca atccgatgtt
     5401 tggcgtcgtc tttttcaatg cattcaaccg ttgccgtcaa ggtatctcca tacaatacag
     5461 gcgctaaaaa gctgagctcc tggttcatgt aaatcgtatc gataccgggc agttcggttc
     5521 ccagtacagc cgaaatgaga ccggctgaaa tcataccgtg ggcaatgcgc tgcttaaaga
     5581 gcgaattctt tgcaaattcc gcattgacat ggaccgggtt gaaatcgccc gtaaccccgg
     5641 caaaagcata cacgtcgtgt tcagaaatcg tcttcgacac cgaggctctg tcgcctacat
     5701 ggatgtcatc gtacgcaata tttcttgcca tcatatcacc tccttaaaag aatatctata
     5761 cttctaagcc catgggctta tcttcaaaaa tacgactgtc catcaatttg acatccttca
     5821 tgatcggctt aaattccatg tgagccaata catctttttc taaatcgatg cccggtgcaa
     5881 tttcaatcag ttccatgcct tccggcgtca atttaaagac ggctcgctcc gtaacgtaca
     5941 gcacgggctg tccgacagaa ttggcataat cgccgctgaa ggtgatttgt tcgacctgtt
     6001 tcaggaactt gtggttatgt ccctcctgat cgatgatcaa ttttccgtcc cggaccgact
     6061 ctttcagacc gccggccgta aaggtgccgc aataaatgac ctttttagag ttctgcgtaa
     6121 tgttgatgaa accgccgcag ccggcaacac ggcctttgaa cttgctgaca ttgatattcc
     6181 cgtgcgcatc ggcttcagcc aggcccaaga tagccaggtc gacgccgccg ccatcgtaaa
     6241 agtcaaactg gtagggctga tcgatgatgg catgggcatt ggtcgcggcc ccgaagcttc
     6301 cgccgccggc aggaacaccg ccgatcgtac cggcttccgt cgtcaagacc atgccttcaa
     6361 caccttcttc tgcggcaatg gaggaaacgc cttcgggcat accgataccg aggtttacga
     6421 cggcatcggg aatcaattcc atggcgcagc ggcgggcaat gattttgcgg ttagtcagtt
     6481 ccatggccgg cagtgaatcg acgggaactt tcaaatcgcc gttaaaggcc ggattgaatt
     6541 catgatccca agtcatgaga tgatcggctg tatcgctttg aacgatataa tcgacgagta
     6601 cgccgggaat cttaatatct ttcggatcga gggtgccgcg agcagcgact tttttgacct
     6661 gagcaatgac gattccgccg caggcatgga cggctgccgc aatggacagg gcttcgccgg
     6721 tcccgatttc ttcttccatg gaaatattgc cgtcctggtc cgctgtcgtg ccccggatga
     6781 tgcccacgtt aagggacagg ggcttatacc aaagccattc ttcgcccttc atattgacga
     6841 cttccactaa gtcttccgtc gtctgagcat ttaattttcc gccttctaag cggggatcga
     6901 cgaaggtgcg caggccgacc ttggtaatcg tccccggacg ctggccggca atatcccgaa
     6961 accactggga aatcgtgccc tgcgggaagt tataggcagc tactttattg tccataacca
     7021 gcttaccgat gcgcggcgtc aagttaaagt gaccgccgac aatgcagcgc agcaaccctt
     7081 cttcgccgag atgattaagt cctcgttcac cgccgtcccc ttgaccggcg gcaaagacga
     7141 cgtttaaatc tcggggcgtg cccgtttcca gataagaagc ctggattgcc ttcaatacgg
     7201 cttcggggat aatcccgccg acaaaaccgc tggtagccaa ggtagctcca ttttcaatct
     7261 tttgcacagc ctcagcagct gttataaatt gtgccatatc aacatctcct ttatcgtaac
     7321 taatctatcc gttagtgaag gcctgtaacc gagtaaataa ggatgactac aaaaacggca
     7381 aaggtcttca agagggtaat tgcaaaaata tttttataag aactccgatg agttaatccg
     7441 caaacggcga gcaaggtaat gacggcgccg ttatgaggca gggtatcgag accgccagac
     7501 gccatggagg ctacgcggtg aaggatttca ggacccatgc cgatagacga ggcccatgcc
     7561 agccagtctt gggacatcaa ttcaagggca atggccatac cgcccgaagc agatccggta
     7621 ataccggcca aggtcgtgac gctgatcgct tccgatacca acggcgtacc gccaatatgg
     7681 atgttgatca aagcgtgggc tacgtgggaa aagcccggaa gctgcgaaat aacattgcca
     7741 tagccgactt ctgatgccgt gttcatgatg gccaagagag aaccgatggc acc
//
LOCUS       DAWDXZ010000019         7782 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3090_length_7782_cov_10.724861, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000019 DAWDXZ010000000
VERSION     DAWDXZ010000019.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7782)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3090_length_7782_cov_10.724861"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1575..1584
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4114..4123
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6253..6262
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1840 a         2198 c         2166 g         1548 t
ORIGIN      
        1 tcgccaacgg cctgaaatac ggcggcgacg aaaatcggat gaagacggcc tttcatgaac
       61 agatgcacgt cgaagccatc ggcttccagc ggtatctcga ctatctcagt gccatcgtca
      121 ccatcgctcc cctgttaggg ctcttgggga cggttacggg catgatcagt accttcagcg
      181 tcctcgataa cggtgccggt gcctcggcca tcagcggcgg tgtcggcgag gccttagtcg
      241 ctacggcaac gggcctctgc gtagccatcc tggccttctg cgtctatacc tatttctcgc
      301 atcagctcga cacgatcgtc accgatacgg aacgggtctg cctcgacgtc atgcaggccc
      361 aaaaagcagg ataggaggcg aacgtcatgt tttctcacgt cagaatgaag aagaagcccg
      421 tgttcatgat catccccatg atcgacatca tcttcttcct cctcgtattt tttatgatga
      481 acagcttgca gaccgtcgcc caaaaagccc tttccgtcca gcttcctcag gcccagagcg
      541 cttcggctcc ggctcagatg cccttgatcg tcaccgtcga caaggacggc catattaccc
      601 tcgacaataa gccggtcagt atcgacgaag cgtcgacttt gatgaagaag cacatggccg
      661 ataatgccaa tgccgccgtc gtcctccagg ccgataaggg cacggctcac ggccaggtcg
      721 tagccgttat ggacatgttg aaatctgccg gcgtcaagcg gctggccatt gcggccaagc
      781 agaaagggtg aggtccatgg acagccgtat ttcttggaaa aaggcttatg gcggatccct
      841 ggtcatccac gccctggtct tcggcctgtt catgtgcttt ttagccagct ccgtcgctca
      901 ccatgaagaa gaaaaaatgt acgtcatcga cctcgatgcg tcggaactga caggcgccgg
      961 gagcggtcat gccggcggtg gcggcggagc ggccgatctc ttccccgata agttgtccga
     1021 cgcggccgtg gccgagcgaa gggctcaggt cgaacaggct tcgtcagcct tggcagctcc
     1081 atcggccata tcgccggtag aaagcgcagc ggccaatccc tctccgacaa attctgcttc
     1141 gtctgatgga ggcagctctg cggcaggcgg ggaaggaagc ggcagcggca gcggctcggg
     1201 aagcggcgaa ggcagcgggt ccggctcggg ctacggttct ggttcgggca gcggcagcgg
     1261 cgacggcatc ggtgacggag aaggctacgg cgaaggaagc ggcagtggtc agggcagcgg
     1321 cgacagcggc agtgacggta cgggtaccgg cgcctttgat accgacggct tttggtccgc
     1381 cgtcaatgcc aataaaacct atcctcccat ggctgtgaag cgcggcctga ccgggacggt
     1441 ctacgtgacg gtcaccctcg acggcagcgg taactgcatc ggcgtttcgg ccagcggcga
     1501 cgggctgctg gctaaagcgg cgatgaaggc cgtctacgcc gcctgccctt atcccaatcc
     1561 gaccgggggc gacannnnnn nnnncttttg aattacagtg aggagaagca ttatgaaaat
     1621 aatcgtactt tactcatccc gcacgggaaa tacgaaaaaa gtggctgagg ctattgccag
     1681 tgccctgccg gccgggacgc cgtgcctgcc ggcagcgaaa gcaccgtccg acatcgaaac
     1741 ctacgatctg gtctttatgg gctactgggt agaccggggg acggccaatg ccgaagccag
     1801 gaccttcctc gaaggactgc accatccgaa ggtcgccctc tttgcaaccc tcggcgcaga
     1861 tccccggtcg gaacacgcgc aaaagagcct cgacgaaggt gcacggctcc tgcccgaagg
     1921 gacggacgtc gtcgggacct tcatctgcca gggcgcagtg gatccgaaaa tcattgaaat
     1981 gatgtataaa aaatttcatg ccggccatcc ccacgggcgg acgccggaac gggatgcccg
     2041 ccatgccgaa gcggccaagc accccgatga agacgacttg cagcgagcct ttgattttgc
     2101 tgccgatatc ctgcactggc tggaaggagg cgggcaatga gcttacaggc ttattacgac
     2161 agtctgtcgc cggaagagca ggccctgcag tttggcctct ccggcggcga tcccttaggg
     2221 cacgccttcc cgagaaagcg cgtcgtccac gccggattga gcggaaagat catcgctccg
     2281 gccgagtcgc agcagatctg gcaggacgtg atgagccgtc ggcctgccgg gcgggacgtc
     2341 caaacggcat acatccacat tccgttttgc aaaacaaaat gcttgtattg cggtttcttt
     2401 caaaacggaa ccgagcagga ggccgaagac gaatacgttc gctgtctcgt cgacgaactg
     2461 gaacaatctg ccgacagccc ccgcctgcgg gacggtttga tccatgccgt cttcatcggc
     2521 ggcggcacgc cgacatcgct gtcgccggct aatgccgact tgcttttgaa aacgattcgc
     2581 cgctgcctgc ccttggccaa tgactacgaa ctgaccttgg aagggcggat ccacgacgtc
     2641 gtcccggaaa agatggatat ttggtttgaa aacggcgtca accgcgtgtc tctcggcgtc
     2701 cagtcctttg atacggctgt ccgccgctcc gtcggccgcc tcgacgacga ggagaccgtc
     2761 cttcgccgac tcgatgacgt aagggcttat aaccaggcgg tcatcgtcat cgatctgatg
     2821 tatggcctgc ccggtcagac catggatgtc tggcgccgcg atatgcgcct tttgacggaa
     2881 tgtgccgccg acggggccga cttatatcag ctcaacgtct tcgacggcag cgacttgaat
     2941 aaagctattt ccgaaggtcg attggaacgg gcggccgaaa cgtccgtcca agcgcagatg
     3001 ttccacgagg cgaaagtcta tttggaacaa cgggcttatc ggcgccttaa tatctgccac
     3061 tggagccgga ccaaccggga gcgcagcctg tataacgtgc tggcccggtc gggggccgcc
     3121 atgtttccct tcggcagcgg cgccggcggt cacgtcgacg gctacgaaac gatgcttcac
     3181 cgggccattt cgccgtatca aatgtttgtc cgccaggggc agaagccctt catggccctc
     3241 atgaaacagt cgcccctgcg gccgctgatc gacttcgtcc aggtccaaat ggaacagggc
     3301 tatctcgatt tgaacggtct catcgccaag gaccgccgct taggcgattt gaagtggctt
     3361 tacgaccttt ggcaggcgcg gggactggtg accgacaacg gcaccctcta tatgctcacc
     3421 gaagccgggc agttctggca ggtcaacctg acccagacga cgctggaatg catgcagtat
     3481 ctcctgacag gagaatcggt catgaactta gctgccgtcg ctgctcaaga ttcagccaag
     3541 tccgaggcca tggcggcggc gatgaaaaaa atgaaagaga agggcgttca gcccggcatg
     3601 gaagccatga aaaagatggc agaagcgatg cagcatcttt caccggaaga actgagcgct
     3661 gtcatgaagc gcatgggttc tatgtaactc ccctatagat gggccggccc ttcaccggtc
     3721 cgaagcgact gctgcccgga gccactccct tgcttcgggc ggcagttttt acttgtattt
     3781 gacaaggttt acggctatcc attaaaatta taatgagagt caatattatt tataaaggag
     3841 gctctaatga atacgctcta tctctatgag gatgaggcaa ccagaaaaat agcccttatg
     3901 gtcagccgaa tggtcggcat gggggcctgc gcgttttatg aatcgcctcc gtcgtggcag
     3961 cgttacggcc gggttgtctt ggtcatgggg gaaaggggag acatgctctc gtcccttccc
     4021 gaatggcggg agcccctttc agggaaagcc ttggttcttt tgcttacggc caagactgaa
     4081 atcagagaag gcccggcggc tgtcgaagag ggannnnnnn nnnggtgttc tgacgactgg
     4141 aagcaacggc accttgcggg ctacgcccat cgagtacgtt ttcgtcgggc aaaagctcta
     4201 tttcttcagt gaaggcggag aaaaattcat tcatctctac cgaaatcccc gggcggcctt
     4261 tgccgtcttc gatgctttta ccgatacgga ccacttggga ggccttcaaa tcgaaggctc
     4321 cgtacgcctg ttagaacccg ctgacccggc ctatcaagct gcggcggccg ccaaggggat
     4381 ttccgaaaaa cggctggcag ccatggccgt cgttctccac ggcatcgaat tgacgccgca
     4441 gcggatgacc tttgtctggt ccggctttgc caaacggaag cagcctttac gccagactcg
     4501 gtgcatactt taaggaggtt atcatggatt atacgacgac gatcaagatg gttcgccacg
     4561 acggaccgct gacggtcctg tctctagaaa atgaaacggg gacggccgaa atgaacaact
     4621 gggaagtcta ccccggcatt accctcagct atctcgaagc ccatatggaa gaattttcct
     4681 gctatgcgcc cctgcggccc gacgtctttg ccatcaatca ctgcgaagaa ggccgcatcg
     4741 aatgccgatt taaaaacgga gaatacctgt acatgggcaa gggcgacatg tccgtcggcc
     4801 gccgcagctg cagcgattac tgccatatgg cttatttccc gtcatcccac tatcatggct
     4861 tgtcggtcct ggtcgatgaa aaacgggctc aatccgtcgt ggaccgcctg ctggaacagg
     4921 agagcttctc tttgatgtcc ctgtgtgacc gcttttgcag cgattccgat ttcggcatca
     4981 tcgttcagga aaacgaggcc atgaagcatc tgttttatga actctaccac gtgccgccgg
     5041 ccatccgcca tcgatatttc cgcctgaaga ttttagaaat ttttctcttc ctcagtacct
     5101 ttgaaggcaa gatgcgcctg gaccgcccgt acctgacgcg gcgccaagtc gaaaccgtga
     5161 aggcggtcca ccaggaattg gtggctgatc tgagctgcca ctgcacgatc gaagcgatgg
     5221 ccgaacgcta caacatggcg ccgacgtcgc tgaagcgctg cttcaaggat gtctacggga
     5281 cgacgatgta tcagtaccag aaagaactgc gtatggctaa agccatggaa ctcttggtga
     5341 agactgaaaa gagcgtcctc gaagtcgcca actgcgtggg ctatgaaaac agcagtaaat
     5401 ttgccgtggc cttcaaaaag acggccggct gcctgcctcg ggaataccgc aagcagcagg
     5461 ccttttcgga ccgattggag gcctgaggtc acaggccggt gaaagcgctc aatcaggcgg
     5521 aggaagacct tgatttgtga aaaaaatgtc gtaatggagt gctttttggc tgaatggagt
     5581 agaatgtgac tacgagatat ggtacccttt taaacgagaa acattttcaa aataactaat
     5641 gaaagagggg acttatcaaa cgatgaggca taaaagcgct gcttatgtaa cttggggcat
     5701 actgatggcc ctgtccctgc cggcctatgc ggcagatgtg caggatacgg aacaaggagt
     5761 gacgacgaag aatgtcgtcg ttacggcgac caggacggaa gcggaggtca agaccgtgcc
     5821 gcagaccgtc gaagtcatta ccgccgagga cattgaaaaa ttaggcgcca ccgacgtcta
     5881 ttcggccctg cggttggccg ataatataca gatcatgaat accagcaccg gcttcggtca
     5941 ccgcatttcc atgcgcggca tggccagcaa cagtgccctt atcctgatca acggccagcg
     6001 gacggccatt gaagatacgt cgacgacaca gaacctgttg gccctcgacc gactgaacgt
     6061 ccataccatc gagcgtatcg aaatcgtccg cggtgccgcc tcggcccagt acggttccga
     6121 tgccctgtcc ggcgtcatca acatcattac caagaagtcg tccggcgaac cgtcggttac
     6181 catgggcgtt gccacgggga cgaccaacat atccaattac taccacatcg atttagggaa
     6241 gcagggcaag ttnnnnnnnn nnaccttcga tatgaatttt tccaaggacc gccagtggac
     6301 ggaacaccaa gtatcgggcc ttccggtcaa gaacctcgaa gggccgaagc agagctacaa
     6361 tttcagcggt acctatgaac tcggcgaaaa taagaacctc aacctcgacc tcggttacta
     6421 taaagataag ctgaccggcg actggagcca taaagaatat aacctcggcg cctggggcgg
     6481 catcgttcgc ctggccgacg ccaagctgga aacggaacgg cgcgacgcca gcctgagcct
     6541 gaccggtaag accgagaacg acgactatat gatccgcacc ttctacagca agctgaataa
     6601 attccgcttc ctgccctata cggccttggc caaagaatac ggagaaacga ataactacag
     6661 catctggggc attgaaggca agaacagcca taagatcaac ggcgaccata ccctgacgta
     6721 cggcacggaa tacaatcgct acagcgtcga cggcgtcaac ttcggaagcg acggaagcga
     6781 cggcaaggac gtcagcactt acgccgccta catccaggac gaatggctca tcggcgacaa
     6841 atgggtcatc atcccggccg tccgctacga ccatcacagc cagtttggca gcaagacgac
     6901 gccccacatc ggggcgacgt acttcctcaa cgacaacaac cgcttcaagg tcaattgggg
     6961 cgaaggctac aaggcgccga gcgtcagcga actctacatg gactataccc acatgggggt
     7021 cctgaccttg ggcaatcccg acctgaagcc cgaagaatcg aagaattggg acatcagcta
     7081 tgaaggggaa tggggcaaga ccttcggcaa actgacctac ttccacaacg atattgaaaa
     7141 catgatatcg acgcataccg tcggcagtgg tcgccgcggc tggagccagt attacaacat
     7201 cgacggcacg accaagaccc acggcgtcga attgacctta ggccgcaaac tgtccgaccg
     7261 ctgggatacg aaggtgacca gcaactggac gtcggccggc aataaatcgg catcggcaga
     7321 agcatcggcc cacggcgtcg acggcattgc cgacaacatc acgacctggc agcttaccta
     7381 cgacgaccat aagaaaacgg gctggaatgc cacgatttgg gaacagtggg tccataatta
     7441 ctacgaaagc gacacccata aagcctacaa ctatcaaacc ttcaacttcg tcatcaaccg
     7501 taaattcaat gaaaagaccc gccttttcgc cggcatcgac aacgtcttca ataaaaagat
     7561 cgacgccatc tacctcgacg gacgcatttg gcggacgggc attgaaatta ccttctaata
     7621 cacctgcttc ttcctcgtaa gtaccggtca gccaatccct ttttccatat agccatgttc
     7681 cggacgaatg ccgttcgggc atggcttttt ttggagttaa cgcgactatt cgtttgacaa
     7741 taaaagtcga aaaacgtata ctaaagaaaa gtcaaagtac ta
//
LOCUS       DAWDXZ010000020         7249 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3283_length_7249_cov_8.148179, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000020 DAWDXZ010000000
VERSION     DAWDXZ010000020.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7249)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7249
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3283_length_7249_cov_8.148179"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3890..3899
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4863..4872
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6422..6431
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6918..6927
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1868 a         1592 c         1882 g         1867 t
ORIGIN      
        1 atcacattgg gtaatagttt gcggcgtgtc ctcctggcat cactggatgg cgtagcaatc
       61 acttcgatta aaatcgatgg tgttcttcat gaattttcga ctattcccgg tgttcgggaa
      121 gacgtttcgg aaatcatcct taacctcaag caggttcgga ttaaattcct ggatgatgat
      181 gtagatgaag ttgacatcac tatcgatatc tctggtgaat gtgaattcac tgcaaacgat
      241 cttaacgtaa actcagacat tgaaatttta aacccggatc tccatatcgc aaccctcgat
      301 acggaagctg acatccacat gcagtgccat atcgaacgcg gtaagggcta tgttccgtcc
      361 acgcagaaca agaaagaaac ggatatgatc ggcgttattc ccatcgattc catcttctct
      421 cccattgtgc gcacgaacta cagcgttggc aatatccggg taggcaacga aatggactat
      481 gattcgttga cccttgaagt aacgacagat ggcagcatca cgactgaaaa tgccgttgct
      541 aaggctgcat cgattatgat cagctactta aactacttcc agaacatcgc taccgatgca
      601 aaggtaaacg atgaaaaagc tatcttcact gacgacgatg gcgaacccgt ggaagaatcg
      661 accgaacccg ataagggcaa aatcaagatt gaaattcttg acctgtctgt ccgcgcttcg
      721 aattgcctga aacgtgcaaa tatttacacc attggcgatc tcaccgaacg gacggaagat
      781 gacttgtcga agattcgcaa tctcggcaag aaatctgtcg atgaaattat tgaaaagtta
      841 gaaatgcaca actactactt aaaatcgagt aatgaagaat agttcgagga ggatgaacaa
      901 tggcttatag aaagttggga cgagacagct ccgcacgtaa agcattgctg cgcagcattg
      961 tcacctcttt attccagcat gaacgcattg aaacgacgga agcaaaggca aaagaacttc
     1021 gcaaagtcgc tgataagatg cttaccctcg caaaacgcgg tgatctccac gctcgccgtc
     1081 aggttttggc atacatgatg gatgaagatg ttgtcaaaaa actgtttgac gaaattgcac
     1141 cgaaatacac cgaccgtcag ggcggttata ctcgcatcat taagactggc gttcgtcagg
     1201 gcgatgctgc tccgatggta atcatcgagt tagtataaac gtatgaatga gaaggagctg
     1261 tcctgagaca gctccttttt gtatatgctc atccggaggt tccggaaagg gcgccatttt
     1321 ctggaaaagg agtgatacta tgccgtatcg tatgatgtta gccgtaaatt tcaccccgga
     1381 aagactggaa caagttaaac tgttggcctt gcttacgaaa tcgaaaatca aggccgtaac
     1441 ggatgaagaa aagggagaaa ccgtcggcaa agtgctgggg ctgaccgatg aagaaatcgc
     1501 cgaaatctct gcgatgagac aggacacgga agaagcgaga gttcatgaaa agatgaatga
     1561 agatgaaaac ctgccgccgg ttacgaaaga agccctcatt ctctgtggtt tcgacaataa
     1621 agtcgtcaat ctcctcttgg acagcattcg ccgaggccgt ttgaaaaacg tacctttaaa
     1681 agccatgctg acacccaata acatttcttg gaccattgaa acgattttgc aggagctttc
     1741 gaaagagcac gaatatttcc ataaaaagag gtaaggacta tgcgtccaac gacactttgc
     1801 tttcccgttc gatcggacgg gaaagttttg ttaggccgaa agaaacgcgg ctttggcgtc
     1861 cgtaagtgga acggtttcgg cggtaaaata gaaggcggag aaactttttt gcagtgcgct
     1921 gtaagagaac ttcgggaaga aacgggttta atggccgatg aaggagattt tgaattggtc
     1981 ggcttcctgg acttccattt tagtgctgca cctgaactgg atcatatcgg ctacgtctac
     2041 atcctgcgcc actggcaggg ggcagtctgt gaaagcgaag aaatggagcc ccgctggttt
     2101 gcacctcatg actttccgta cgacgagatg tggaagggcg accggacctg ggttcccatg
     2161 gtactaaaag aagactgtgt gaagggcatc attacctttg ctcccgatgg agaaaccgtt
     2221 caggatatgg aacttcagcc cttttatccg cagcggctcg aagattagtt tttggctgaa
     2281 aggcggcaga ccggaatgaa tcgtgatttc gtgatttcac aatttaaaag ctatgtggca
     2341 aattttgacg ctgtcgatga gggaatttcg ctgaaattcg cccattcttt gcgggtcagc
     2401 gcttggtgtg aaagactggc aagggcctta cacctttcgc aggatgacgt ggatttagct
     2461 tggattatcg gtgtccttca tgatatcggg cggtttgaac aactgcggga gtaccatacg
     2521 ttcatcgatt atcagtctat ggatcatgcg aaatatgggg cacggtatct atttcaggat
     2581 ggtcatatcc gtgatttttg gcaggatgac agtcaagatg ctgtcatgga agccgctatt
     2641 tctcagcata atgtgtatcg actggccgat gacttatctc cgcggcagcg actgttttgt
     2701 cagctcatcc gcgatgccga caagcttgat atctttaaag tctgtgccga tggcctgcgt
     2761 aaaggtcaga atgtgtggcg tgtcgatgac agcgacgttg aaaaccagcc tatttcagag
     2821 gcagtcatga aagaagcccg gcagaaaaaa ctcgttccga cccaggtgaa aaagactttt
     2881 atggattttt atgtcggtac cctttgtttt tattttgacc tcgtctatga tgagagccgg
     2941 aaactggcca gggagcaggg gtatttcgca gagcttctcc gttttcattc acaaaatccg
     3001 gacagtgagg cgaaattgaa tgaaatacgc ttcctcatcc aaggcgactg aatttttccg
     3061 gcatctcgat gtcggatcct atcaatacgt aatggagggc ctatcaacgt ggaaattttg
     3121 attactggcg gtgccgggtt tattggatcg catatcttgg aagctttaca ggggcggccg
     3181 gatataaagg tcgtcgtttt cgacaattta tccggcgggt cccgcgaaca tgtaccggcc
     3241 gggatggagc tgattgaagg ggatgtctgc gatcaggaag ggctggatac cgtattcaga
     3301 aatcattcct ttgcggctgt catccatttg gcggcccaga cgatggttcc gttctctgta
     3361 tcgcagccga ttgtcgattg ccggattaat ttagaaggcg tccttcaggt cttggaagga
     3421 tgccgaaaat acaaggtccc acacattctt ttttcatcga gtgctgccgt ttatggggat
     3481 aatttaaata ttcctctgga tgaaggagag cgcttggtgc cgacgtcgcc ttacggtgtt
     3541 accaagatga cgacggaaca ctacttgaga gtatatcatg agctgtatgg gttggacgct
     3601 acggtgtttc gctttgccaa tgtctatggt gaacggcagg gggaaaaagg cgaaggcggt
     3661 gtcgtcagca ttttctgtaa actcttgtcg gaaaatcaag ggattaccgt ctatggtgac
     3721 ggcaatcaga cgcgggattt tgtctatgcc ggtgatattg cccaagctat tatccggtcc
     3781 ctgcctctta cgggctatca tacgataaac gtctcgaccg gaactgaaac cagcatcaac
     3841 gaactgatcg aaagctttga aaaagccgtg ggtcatgccg ttcctgttgn nnnnnnnnng
     3901 cccgtaccgg cgatatcctg cggtcggtct taagtaataa ggctttggaa tcgctccttt
     3961 cctttgtgcc tccgacggat cttgccgaag ggattcgccg cacctacgag tggtactgcc
     4021 gtgccgctgc taagtgaggg gcatcgcctt gacaggtatg gtaaaacatg acacaattaa
     4081 ttagatatgc ctatataagt gtacaggagg agagctcatg gcagatgata gaatcattgt
     4141 ggctttggat gtccattcac tggaagacct gaagaagctc gtagaaacac tgggcgaccg
     4201 tgtttctttt tataaagtcg gcatggaact gttttacagt gccggacccg atgcggtgcg
     4261 gtacttaaag gatcagggaa aacacgtttt cctcgatttg aaagtacacg acattcccaa
     4321 tactgtcggt cagagtattc gtgccttgac ccgccttggc gcagatttca tgacccttca
     4381 cgggaccggc ggccgggcta tgatggaagc ggcagccgaa gccgtccatg atgaagcgga
     4441 aaaattaggc atcgaacggc cgcgtttgct ggccgttacg gtattgacca gcatcgatga
     4501 agacgcttgg aaagagatcg gcggtcgaga ctgcattgct gattcggtaa agaatttagc
     4561 gaaaattgcc aaagaagccg gtatcgatgg taccgtatcg tcgccgtatg aagccaagga
     4621 aatccgttcg atgaacgggc tggacttcct catcgtcacg ccgggcattc ggccgacttt
     4681 tgccgttgcc aatgaccaaa agcgcttcac gacgccgtct caggcactgc gcgacggagc
     4741 ttcccattta gttatcggca ggccgattac caaagcggac gatccgaaag aggcagccgc
     4801 aaagatttta gcagaaattc agggggtata acttattatg ttgacagaag aagaagtaag
     4861 acnnnnnnnn nnaatgtaca gaccgttatg ggacccatga ccggcggtat cctcttggcg
     4921 catgaagtcg gcaaagccct gggtacgcgg gctatcttta cggaacgtga aaaaggcgtc
     4981 atgactttgc gccgcgggtt ccatatcgaa ccgggtgaac gggtccttat cgtcgaagat
     5041 atcgtaacca ccggcggatc ggtccaagaa gtcgtcgatg tcgttaaaaa agccggcggc
     5101 gtcatcgtcg gtgtcggcct cctcgtcgac cgcagcggcg gcaaggctga attcggcgtg
     5161 ccgaaagaca aggtacaggc cctgctgcac ttgacggttc cgacgtacaa gccggaagaa
     5221 tgccctctgt gcaaacagag tatccccatg acagaacggg gaagccatca tttaaaataa
     5281 tctgggtcag gagttgtttt atccatggaa atgttatcgg ccatcgcccc gttttttgtg
     5341 gccggtttga tggggaccat atttgcccgg tttacgggaa tgaatatgag catgtgtgtc
     5401 ctcctcctgt ttttgtatat gggagccaaa ccggtcgaag ctatctcggc tatgttgatg
     5461 tttaatgtct ttacgtattt taccgtctat tcgcagcttc acgtcatgcg gatcaaaagc
     5521 tttgcctttt tccccggcgt gcggctggcc attccgatct taattaccgt cggcgtggca
     5581 gcactgaatc cctttgtcgg tatcgctttc tttatcctcg tgttcctgct ggaaatcttt
     5641 gccaagattt accgggaaat gaatcccaag tcacggcctt cgaagtggca gatcgtccag
     5701 atggtcattc ctgccggtat cttagtgacg ataggcgcgg ctgcagttca gttcattccg
     5761 gaaaattatt actatatcgt cggcggcgtt gccattttgc tgtatgctgc catcatgtgg
     5821 cgcatgggcg atcggcgtaa gggtacggct tggtgggaca aatggctcta cctgtcggct
     5881 ttgcttaccg gcctcagcgg catcgatggg acggattgga ttacggctat gcgccgcaat
     5941 cctgaatcgg tcctgtctaa atgcttcccc atcgtcatca atggggccat gttgattgcc
     6001 ctggtggcct cttacgccat gtaccgttat ttttcaatcg gtgccttatt tgccaccatc
     6061 ggtacggctg tcggtattcg cttgttcggt ctatatgaac attccggtaa gggcagtttc
     6121 tcgtacctca ccttgggagt cgccgtcttg acagccttag tgttcatgat cgtccagcct
     6181 gttcctacgg ggttccccct cgtgcctatg gctgatgaaa caggtttctt tagcttttaa
     6241 atgagtgagg atgcatcgtc ttgatgggtt ctccttgcat taggtatgag ccatccggaa
     6301 atatcataat aatgtgccat ccccttacgt tagatttaaa gatataacgt aaggggatat
     6361 tttttagaaa agtgtagtta gaagctagag agctgttgtc ataaatattt acaagggaga
     6421 cnnnnnnnnn ntcaagacaa ctgggatgca gcggcggttg attttgccgc catgctggaa
     6481 aaatccctgg ccaagaccga taatctgctt acttctagga ataactttcc caaaggtatg
     6541 attaccggat ttgccaagaa tgctcccgag gaagtccgcg ccatgtatag ggacctcttt
     6601 gatgagcgta aggacgtcta tgatcgcatt gctgccttta agaagcagtc ttctgttctt
     6661 ctcgatacat acggcaacgg agctaaacag cactatcaaa atgaaaacgc cataacaacc
     6721 tatctttggc tgcggtatcc ggatacatac tatatttata agctcagcga ggtaaaaaaa
     6781 gtagctgatg tcttaaacgg tgattatcgg tttaagaagg gagcctatag ggataacctt
     6841 caacattttt acaaactcta tgatgaaatc tgtgcggaac tgaagcaaga tacggagctg
     6901 gtggagctct ttcgaaannn nnnnnnngat atgggacctt agcgaacgaa tataataaag
     6961 atagagagca taaggcgatt ttatgaatcc ggcagcaatc gtggcggcca aggaggcgtt
     7021 cggttctgtc gaatcgaaac cggggaagat catcttttac aagtgagtgt ggagatgaac
     7081 ttttgattac cgtatgttac ttggaaaata tataatactt tgcaaggtct gaggaggtac
     7141 gcttgatgtt cgataaatct aagttagcgg acgttctggt tcgatacaag aaagatttct
     7201 tgcctaagcg ctgggaaagg gaaaaataca aatgggaagc agttaaatg
//
LOCUS       DAWDXZ010000021         7225 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3293_length_7225_cov_8.665969, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000021 DAWDXZ010000000
VERSION     DAWDXZ010000021.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7225)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3293_length_7225_cov_8.665969"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1023..1032
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2350..2359
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2724..2845
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4129..4138
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6453..6462
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1791 a         1936 c         1625 g         1711 t
ORIGIN      
        1 cctgccgggt atcaaagtag agaccgtcgt ctgcgaagtt cccgtcgaca aagtcgtcga
       61 agtcgctgaa agggtctgca aaacaggtaa gcccggtgac ggtaagattt tcatttaccc
      121 cttggcagat gccgtccgca tccgcaccgg tgaccgcggc cctgtcgcca tcatggacga
      181 aaaataagaa tgtctctaaa agcaggtatg cgaagcacga gccttcgtat gcctgctttt
      241 taggctaacc gctaaatcaa tagtatatat atatcaatat atataaaata agtaatagta
      301 cccgtcgacc ttcttgttta aaatagatac atacttgctt atcagcacag tagcgtatgt
      361 gaatatttta acaattcgaa gggatgatca tatatgacga acttacgtcc taagaaaacg
      421 aagaaacgct ggttcatgcg gtttctctta tttattctca cagccatcaa ttacctggac
      481 cggaccaaca tggccgtcgc agcctccagc atgagtgatg aactcggatt cagcgccgcc
      541 accatgggcc ttttattctc cgccttttcc tggtcttacg ggctgatgca gatccccggc
      601 ggttgggtca tcgaacgatt tggctctcac atcgtctata cagcctcgtt attcttgtgg
      661 tccttattca cgatgttcat gggtcttggg aagtcctttg cctccctctt cggcatccgc
      721 atggctatcg gcgctgccga agccccggcc tttccgacca acagccgcgt cgtcgcagcc
      781 tggttcccgt ctcatgaacg ggcgacggca acctcgattt atacagccgg cgaattcatc
      841 ggtctggcct ttttaacgcc cgttttattt tggatcttag aaacctacgg ctggcgggaa
      901 atattttaca tcaccggctt tatcggcatc gtcgccagcc tcggttggtt ctttctctac
      961 cgcgaacccg gtcccgattc aggtgccaac caagctgaat tggactatat ccgccaaggc
     1021 ggnnnnnnnn nngcctcgga aaaacaaaag attacctggt ctcaaatcgc agaattattt
     1081 aaacaccgcc aactctgggg gatttattta ggacagtttt ccaatgccag tacgatgttt
     1141 ttctttttaa cctggtttcc gacgtatctg atcatggcta agaacctgcc cttattaaaa
     1201 gccggcatct acgccatcat tccttacgtc ggtgccctta tcggcgtcct cctcggcggc
     1261 tactggtctg actccatgct gcgccgcggc agttcccttt caaaagccag aaaactgccc
     1321 atcatctgcg gtctcctctg ttcgatgacc atcatgacgg ctaattattt cgactctctc
     1381 aatgccatca tcatcgtcat ggccctggcc tttttcggtc aaggcatggc tgccatcgtc
     1441 tggagtactg tcggcgacgt ggcaccggcc cattccgtag gccttgccgg cggcgtcttc
     1501 aacttcatcg gcaatttagc cggcatcatt acgcccatcg tcatcggctt tatcgtccaa
     1561 gcgacgggct cattcgtcgg cgccatgata tttgtcggct gcgtcgccgc cattggcgtc
     1621 ttctccttca tcttcatcgt cggtgacctc caccgcatcg aggactgact cagatggctc
     1681 cccgctgcca gcgggacagg agaaaattcc ggaaccgttc tacgacgggc aatacctggg
     1741 attggcgcag gagagccaaa aagagataac gccgttcaaa agaatcggca atcggtatcc
     1801 gatctagatt ataatattga agtgtataga aatcaggtaa aatggcaacg ccatagccgg
     1861 ccgctacaaa gccggccatg gcgttgtctt cttctacatg gcaggcggtc tttaaatgaa
     1921 cctgagcctg atcgaggagt ttattaatca tctcgtttaa gccgctgctc ttatcgaagg
     1981 cgataagagg atacggttcc agttctttta aggagacaga gccacggttg ctcaaagggt
     2041 gccccttagg gacggctacg accaattctt cttcggccac gggataaaag aggacgtccg
     2101 gttcgttttc aacggaagaa cagagagcgg catcgatttt ttcctctttt aactctttaa
     2161 tcagggctgc cgtcgtccct tgatggaagt caaaggtaat gccgccatta ccgtcaatag
     2221 cctgaaattg agtaatcatt tctggcacca aagtatagcc catggtataa ataaatccca
     2281 gcgtaatttt tccctgatcc gggcgcgtat aagctttcat caaatcaacg ccgcgttcca
     2341 gttcctgcan nnnnnnnnna gagcgtttca cataaggcaa aaaaagatga ccgtacttat
     2401 tcaaaaaaat atttcgtccc cgtttttcaa agagctgaat tcccagctct ttttccattt
     2461 cccgaacggc tcggttaagg ctgggctccg taatatacag ggcttccgca gccttccggt
     2521 aatgttccgt ttccgccaat ttcgtaaaat attgaagttg ctttaagttc ataacagact
     2581 ccttctatat gccgtttaat caatctttgt gataaatcct tatatatcaa tagcttatct
     2641 attaatattt caatctcatt ttctatcaat aaaaaacaat cttatgaaaa ccccaaatgg
     2701 caagacaagt caaagctata tggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnncaact tccttcgtat tgtaaaatga gtttatcttt
     2881 gttttatcga caatttctcg gtatcgattg cccttattat atcattttta atagcttttt
     2941 tgaactgttt ttgcctattt taagaaattg tgttatcagt atttcatccg tataataagg
     3001 gcagatcaaa aatatagttc tacaattcac atataccttt agtactatat agaaggagga
     3061 tttctcttta tgagccagca gtatccacat attttcgaac cctttacggt caagcggacc
     3121 accttccgca accgcgtcat catgcccccc atgggatcca atcttgccac cttcaccggt
     3181 gaagtatcag aagaaatgaa gaattattac ggtcttcgcg cccgtggcgg cacagccttg
     3241 attaccgttg aaaacgcctg tatcgactat cccttggcaa cgaacggaac gaaacagctg
     3301 cgcatcgaca agaagcagtt catccccggt ctctacgaac tgaccgaacg gattcacaaa
     3361 tacggtgccc tggcatcgat tcagctcaac cacgccggtg cttcggccta cccggaacgg
     3421 ctcgacggcc tccaagccgt atcggcttca tccgtccctt ccaaagaagg caatccggca
     3481 ccgcgccccc tcgataaatg tgaaatcctc aacattgtcc agaagtatgc catcgccgct
     3541 cagcatgcta tccaggccgg cttcgacatg gtagaaatcc acggggggca ttcgtacctg
     3601 ctggaccagt tcttatctcc cctgtataat aaacgtaccg atgaattcgg tggctcttat
     3661 gaaaatcgtg cccgctttgc ccgcctcgtc gtcgaagccg tccgcaaagt tgtcggccct
     3721 tgggttccca tcgtattccg tctgtcagca gatgaattca tcgaaggcgg caacaccttg
     3781 gaagactcgt tgaaactcct cgaatacatc gaaccggaag tcgatatcat caacgtatcg
     3841 gctgccgtca acgactccat ccagtaccag atcgataaat gcgacatgcc tgacgcctgg
     3901 cgagtctacc tggccgaagc cgtcaagaag aaattcaaca aaccggtcat cgcttccggc
     3961 aacatccgca atccgcaaat cgctgaccag gtcattgccg aaggtaagac cgacttcatc
     4021 gccatcggcc gcggcctcat cgccgatccc gattgggccc gcaaatcaca gttcggccgt
     4081 acggactgca tccgacattg catcaactgc tacatcggct gaggctatnn nnnnnnnncc
     4141 ttcgggaaag acgccgatat cgtttccaaa gcgaccttca aagagctctt agaaaagcac
     4201 cccgttgaca tgcatctcag cacgtcctta gaaaaggtct gcccggacaa attcattgcc
     4261 tcccacaacg gtgaatctgc cgagtatccc ttcgactacg ggttcatttg catgggcctt
     4321 gttcccgaca tcccgtcgga taaccccttt gaagcctatg ccaaagaaca gggaatcccc
     4381 ttctgcaact ttggcaacag ccgcaagacc ggtcagatta tccacggcac ggaagtaggc
     4441 cgtgacatcg tcgctacgat cgacatgatt ggcggcttcg acgactaggt aagcagaaaa
     4501 caggatactg ctgctgtttc aagcggcggt gtcctgcttt cctatctttt tgtatccctt
     4561 catggaaaga cgattcaaat attaaaatac gatattcctt tattctcaca acaggaggat
     4621 tctatcatgg gtaaaattgc agttacagaa cgcgtcacgg gtcatacgga attaatcggc
     4681 ctcatcgcct atccgattcg ccacagcatg tcaccgacga tgcataacga agccttcaaa
     4741 aaattgggcc tcgattacgt ctatgtctgc tttgaagtcg acaactcgac gctggaagac
     4801 acggtaaaag gccttcgcgc tatgaaagtc cgcggctgga acgtttccat gcccaacaag
     4861 gggccgatta cgcagtacct cgacaaattg tcgccggcct ctcagatcat tggcgcctgc
     4921 aacaccgtcg tcaacgataa cggcgttctg accggtacca ttaccgacgg cgtcggcgcc
     4981 atgcaggctc tcaaagacga aggtgttgaa tacgtcggca aaaaaatggt cgtcatcggc
     5041 gccggcggtg ctgcaacggc catccaggtc caggccgccc tcgacggtgt ccgggaactg
     5101 acgattttca accgcgacga tgaattcttt gcccacggcg aagaaacggt caagaagctc
     5161 cgcgaacgga cggactgcgt cgtcaatatt taccatcttg aagaccacgc aaaattaaaa
     5221 gaagaattag acagcgccga catcctcatc gacagctctt cgtgcggcat gaagcccctc
     5281 gaagacgtcg tcgcccttcc ggataaatcc tatctccgcc cggatttggt cgtcatggat
     5341 acggtctatg ctccgcgtca gacgaagttg atgagatggt gcgatgaagt gggctgcaaa
     5401 aacttcaacg gcctaggcat gatgctctat cagggcgccg ctgccttcaa actttggacg
     5461 gggaaagaaa tgcccgtaga cttcattaaa gacgtccttt tctaaaccgt cctaaccccc
     5521 gagagacgtt gtcaggctgc cggccgtccc cctgacgata gggccagctg ccggccgtcc
     5581 ccctgacgat agggccagca gccgccggcc ctgacaagtt ttctcattgt ctgcatcatc
     5641 aacgtgcgtt ataatagcgt catgaaagca taggaggatt taaaatatgg ttaaaattgg
     5701 caacgtcact ctcgacaacg ggatgcctaa aatctgcgtc cccatcgtcg gccattctgt
     5761 cgatgagctc gttcaggagt gccgttatct ccaagataaa atttacgatg tcgtcgaact
     5821 ccgtatcgac ttcttaaagg acgtcacgtc cctcgaagcc gtcggcgaag ccctggatgc
     5881 cgttcgccgg gaacttccca acggagccat tctgttcacc ttccgtacaa aggaagaagg
     5941 cggtgaaaca gccgttccgg aaacttacta cttcgacctc atcaactacg ccatcgaaag
     6001 cggtaaagtc gacgccgttg atgtcgaata cttccgcagc cgcgaaagca tcgagaagac
     6061 cttggctgct gccaaagccc acaacgtcac cgtcatcatg agcaaccatg atttcgacaa
     6121 gacgccgtct ttcgatgaca tcaccggccg cttgacgggt atgaaaaagc tcggcgccga
     6181 cgtggcaaaa ttggcctgta tgccgacgtc tgccaaagac gtcctgacct tgctgtcggc
     6241 gacggaagcc gtaaaagccc agtatccgga tgaaccgatc atcaccatgt ccatgggaaa
     6301 actcggtgcc atcagccgta tcagcggcga aatcttcggt tcggccctga cctttggttc
     6361 tgccaaaaaa gcatcggctc cgggccagct cgaagttacg acactgcagc aaatactgcg
     6421 agcccttcac taatagaata agacataaaa tannnnnnnn nngttacgat accggccaaa
     6481 ttaccggcaa agctgaatac accaccggcc aggcccaacg attccttggg agcaacgtcc
     6541 ccaacggtac tccaaatgat ggcggccatg ccttggccaa aaaaggataa ggccatgata
     6601 gcaatgatga tgtccagtga atcaaagtaa ttcgctgcca tgatcgacat ggagcagatc
     6661 aagccgcagg caataggcag ctttctggcc ttcgataaag aagcgccttt cttcaacatg
     6721 tgatcggacc agcgaccgcc gaggaggaca ccgatgaggg cgccgaaata agggatgatg
     6781 gcataaatcc cggcttttaa cataggaaga ttttttgcca taatcaagta cgtcgggaac
     6841 caagtcaaga agaagtacat cgtgctcgta ttggcaaact ggctgatata aatgccgatg
     6901 agctggcgat ggcgaaacag ttctttgagt tgagccatgg tgattttttt agacgtaccg
     6961 gcctcttcag cctgtccccc accttgacga atatagtcga gttcagcctg attgacgcct
     7021 tggcagtcct tcgggtcccg gtatagccaa taccaaatga cgctgactcc gataccgatg
     7081 acaccggtaa tatagaagac ttctcgccag ccgaaggtat ccaaaatcca aaacaaaacc
     7141 ggcgttaaga aagccaagcc aataaattca gcagccgtat agatagacgt cgccgtcgcc
     7201 cgttcatgat cggggaacca ggccg
//
LOCUS       DAWDXZ010000022         7191 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3306_length_7191_cov_8.488229, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000022 DAWDXZ010000000
VERSION     DAWDXZ010000022.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7191)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3306_length_7191_cov_8.488229"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    833..842
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2157..2166
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6075..6084
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1654 a         1902 c         1853 g         1752 t
ORIGIN      
        1 gtattcgaga gctggaagtc gacatccatc gactcatcgg tcttaccgcc ggaaagggca
       61 ctgacaggaa tcgtcccttt ggcgctgatt ttataagggt cgcggctggc actggcttgc
      121 tggaccgtaa tcatgccgcg acgaatatta aacagggcaa aggcaccgct gatggaaatg
      181 ccattggccg taccgcttcc aagctgagcc gatacgtcgg cctgaaggtc atcagaggtc
      241 ccgctgaggt cagcggcaaa atcgatgggc gcatcgacga cgaaatcagt cttgcccaat
      301 acagtcatca aggccttaga cgggaactgt ttcgctgcca ccgataaagc caccgggcca
      361 tgaagggcat aggaaccctt ggccgctaag gtgccgtctc cgattttaag agccagctta
      421 ttgatattga cgatgccttg ttccagtctg acgtcgacgt cagacggatc gatggtttcg
      481 ccgccaaaag taccttggct gatattcccc ttcatagaga ctgtcggatt gtcactggta
      541 ccgctgatat caatctgacc gttcagtttc ccggtaatat cctgtaccgg catatccgcc
      601 actttaatca aaccggcaat gtcgccgttg acgaccgtcg cctggccacg aataaaggag
      661 gttttcgtat tatacatcaa attggcatca taattgccgt cgagctgtga gaaatgcaga
      721 ttggtcagcc gcagtatgct gtcgtaatag gcaaaatcac catggacgtc gttgaggacc
      781 atatgattaa taaccaaatt atctgccttc aaagccccgg atgccgtcgg agnnnnnnnn
      841 nncgtcggag cggacatagt tcctccaata tgcgccgtca gatcaaaaga accgctgcgc
      901 ggcgtctgat tctgcggggc cagtcgggaa acgtcgatat ttttcccggt cacatcgaga
      961 tccagcgtat caccaataga accttccaag gctaccgaag ccgtatagga cgaaatatcc
     1021 ccgttcgaca aatagacggt tccctgattg aggcggtagt cagcactgat attcttatac
     1081 aagtaaccgg cataggaacc gctggtcagt cggaaggaac cggcagccga cggattgtca
     1141 agggttccct ttatcgtcac cgtattatcg gcccagcccg tgagcggcaa atcttccttg
     1201 ccgacaaggg gcagcagttg ttccacgcga atatgggacg tcgtaagcga agcgtcgaca
     1261 gtccggctgt ccagatcggc acgaccggtc aaggtatgca ggccttcacc gcgccgccag
     1321 tagagggccg gaatttcaat ctgttttccc tgaccgtgag ccgacccgtt aatgctgtca
     1381 aagagcatcc ctttgatttg gccgtctttg gcactgaagg ccaagtccca attttgtccg
     1441 tcaatatgtc cggcagcatc cgcggtcccg ctcataggaa cagacaatag cggcgaagcc
     1501 atatcgaggg ggatatcatg ggctgtaaag gaaaattgcg gtcgttgttg atcgacatca
     1561 taggagccgc tgatatcaag actgccgccg ccgacatcgg catgcccttg attgacgttg
     1621 acgacatggc cgtcataggt cacatcggct tgagcccggt ccgctgccag tccattgtag
     1681 gaaagattcg atgcctgtac cgtccccgtc gccaggatag tgccatcctt actgtttccg
     1741 ccagctcgga acgagcccga aacgaggccc ataaccccga ccggcatatc ggggacagcc
     1801 gtcaaatcaa ggccgcttac gtcggcttcg gcggcatagg cacctgtatt tacatcatat
     1861 tgggccttcc ccttgatgta ggcgccaaaa gcccgggccg tcagagaatc gacggtcacc
     1921 atctgaccgc tgtagttgac gtcagccgaa gcctcatcga cggtgtagcc gttataggca
     1981 aagttatgaa taccggcagt tcccttcact cggacatcct ctgtcgagcc ccagacaata
     2041 cctttaaaat ccgccgtccc gctggttcct tccgggatgt agcctgcgaa agccgtcaca
     2101 tccgcaccgg gtacgtcgac gttgagattg aaaacgggaa catccccatt ggttacnnnn
     2161 nnnnnntggc caagccctcg actcctgaca gggtaacgtc gttggtcgta agatcggcat
     2221 ggccttttaa ttcgtcaatg gcaacgccgc ttacatccaa tttaccgtcc gcaaaatcgg
     2281 cctgaccgga catgaaaaag cccttgtgac tgtcagcaac gcggaccttg gcatttttga
     2341 cagtgccctt ctgaaggacg acgtcgtttg aggccggaat gaggaaggac gcataccggg
     2401 cgtcgacggc atcggcattg acgacaaagt catactgccg actggaagca taccttccat
     2461 gagccgtgat tttctgtccg tcgacattca tatcggcatc gatgtccatg gacgggtact
     2521 tggcgaagga aacactggcc ataacgtcgt tcccgacgac gagggtatta tcaggcaaca
     2581 gcgcccgtac cgtaccgtca tgaacacgga tcacgccgcg aaagccggaa tcactgctgc
     2641 tttccgtcgg tttcaaaatc gacgtgatat tccacgatcc gtctcgattc tgccacagat
     2701 gaaggaccgg ttcattgacg tcggctgtat ccaatgcctg gaggatttcc ccttgcttta
     2761 acgccgttac aatcttaccg ggattgacat agatgcggac gtcctcgcct tcgaaaacga
     2821 tgcggccctg cgaatcttta ataaccggtt ttgccaagac cacttggccg gtcaaagata
     2881 tatcaagaga agagaacccc aaggttccat tcagtttact gtttgcagtt gtcgtaatca
     2941 tattgctcgc catttgggtt aaagccggtt tttccatcca caaggcaatg ccggcagata
     3001 gaaaaaaagc ggaaaaaccg caggcagcaa tccatttttt cttcataagc aagctccaca
     3061 tacgaaaaaa gagagaatta cacgttctaa ttctctcttt attctaccta tggtattgcg
     3121 gaaaatcaag aaatagatga caaatcggaa cgtttctccg aaaattgtta aaaacctgtc
     3181 atcgtcctct tcccttaata ttgaccgcct gttccgttca aagacggatg gatgacgcct
     3241 gattcgacgt cgacacccat gacttgttca agtttggcaa tgccgacgtt gtagtcgtac
     3301 agggcctgga tgtggttgac ctgagcttca ttgaggctca gctgggcatc aagaacgtcc
     3361 aggttgatgc cgacacctgc ctgatagcgg atgttctgaa tacgatagtt ttcgcgggcc
     3421 tggtcgacaa tggtctgcgt ttcttcgacc ttttgcgctg cgctgcgaat attgaggtag
     3481 gcctgcttga cggcgagctg taccgattcg cgcgtggcct gttccgtttc ccgtgcaacc
     3541 aagaggtctt gtttagcctg gttgaccttg gctttagtcg cgccgccgtc atagaaggtg
     3601 taggttacac cggcgccgat attccagctg cggcggtcgg aatcgacacc ggggaagtta
     3661 ttatccgacc agctattgcc gccggtaacc gaaaccgtcg gtttattgcc ggcgtcggca
     3721 atagagatgt ttcgttcagc ctgctgtacc gcaagggctg cctggaggac ttccgggcgg
     3781 tatctcatgg cgtagtccgt ggcttcctga agggaaatgt tgtaagtatt aaagggcagt
     3841 tgatgatcgg ccagattgag ctgcgtctgt aacggcaggc cgaggatatt atttaaattc
     3901 gattcggaaa tggccacatt attttcggcc gtaacggctg tcgtcttggc attggccagg
     3961 gaaacagcag acgacaagac gtcgacgcgg gctttcgtgc cgacgttgta ctgggcctgg
     4021 acgttatcta aatggccctg gagattggta acggcctcct gatagacgcc ttgtaattcc
     4081 tgataggcta agagggcata gtagccttca acagccgaat atttcgtcgc ctgttcgaca
     4141 cggaggattt cttcctgggc atcgacttta cccaatttgg caacgtcaat actgccttcg
     4201 accttaccgc ccgtatagag aggaatggtc acagccaggg agtttccaaa ggcattggaa
     4261 atggcatttg tgctccggga cgaagcgagg ctggcgttcg tagcccgcgt acgcgtagcg
     4321 ctgaaggtgt agtcgacgac gggggctctg ccggctctgg cttcatcgat ggcatattct
     4381 gccttgcgca gggagtactt ggagttcttg atatcgcggt tgtaatcgag ggccatctga
     4441 accgttctgg gcaaggtcaa atcgacgacg ccgttcgtcg gcacgcggcc gacgacgatg
     4501 accggatcga tttcattttt cttagcccgt ttagccgctt ctttctcagc cagtttcgcc
     4561 gaatcatcgg caatcttttg ggccagtgtt tcacgcattt ccggcgtaat atccgcggtc
     4621 tgggcattgg ctttggcacg tgccttcgct tcggccttag cagccttttt ctgctgtgcc
     4681 tgctgggctt tgatttcctt atcttccgct ttagcccgag cctggacttc atcggcctgg
     4741 gacagatcga caggacggac cgatgccgtc gtatcgtcag cggacgcaac ggcgactgtg
     4801 cttatggttg taatcatcat ggcgatgacc aatttcttat acagtttgtt gttcatcatg
     4861 ggtaccccct gttttcttta tatagaattt gctaaaaagc ataattaata ccataatagt
     4921 tgccgccatc gcgacgactc atgggccgcg tgaactggct gaaaaggtag agatccgacg
     4981 tcagccgata ctgggctttt aagcgaaaac gccagtcatc gggatcgtac ccttcaaggg
     5041 acaggcgaaa aggcccgtcg tggaataaat cagtaccgat accgaggtcg ccgttgatca
     5101 atccggccct caaatcaaag atgccgttac gacggccgta ctgtaagttg aggttcgtac
     5161 cgttaccgat attttcagct ccgatcaagc caaagctatc gccgcggtac accctaaaat
     5221 ttacattgcc gctcgtttcg ttcttcgtcg tgttatataa gagtccgcct tcgccttcga
     5281 ctttgatatc cgatgaaccg ccaaggattt tattgacctt ggtcgttatc tgggccgtat
     5341 tatcgagcgt cgtctggata ttggcctggg tcttggggtc agtcgtcatc ttttccatag
     5401 actgggcgat attttcaaag cgatcggtaa tcgattttat attggccgcc gtttcccggg
     5461 cattggccga ggtcgcaccg tcaccatcga gctgctgcat agacgcattc atttgcgccg
     5521 tcaccgccgc catgttagcc gtaatcgctt caacattggc cgcgttggca tcgagcatct
     5581 gactggtccg gcccgtaata gcatcgatat tttgaatgga accgcgcatg gcagcctgag
     5641 ttttttcatc accgataacg gcattgaggc tcttgaccat ctcatcggcg ctccccatga
     5701 gtttgctggc gctgtccatc atatcgtcca tggtcttgcc ctgcttgccg tcgatataat
     5761 cgccgtcagc taagaggtgg ctgcggtcgg caccgggaga aatagaaacg attttctccc
     5821 ctaagagacc gtccgttgta atgacgattt tcgagtccct cggaatttct gttcccttat
     5881 cgattttcat ggtcacgtcg acgccgtttc gcgacggcgt tactttttcg accttaccga
     5941 catggacgcc tacgtagcgg acagaatttc ctttttgcag cccgttggca tcattaaatt
     6001 cggtatggat gacaaagccc ggcttgccaa agagttcgat atgggccagg cccatgacga
     6061 cgccgctgaa taagnnnnnn nnnnactttg gcttctgagc tccacttcat agactatccc
     6121 ctctttcttc cggaagacga ccgccgtgga taaagcgttg aacgtcttcg tcatcggttt
     6181 gggcaaaact ttttttatcg gcaatgagcc gaaagcgccc atcctgtaaa taagccaacc
     6241 ggtcggcgac aaaaaaagcc gaatccatat cgtgggtaac gatgaccgag gtggcgtgaa
     6301 tctgtttttg catgctgacg accagacgat tgatatccat gcagcgcagc ggatcgaggc
     6361 cggccgtcgg ttcatcatag aggatgacct gcgggtcgag ggcaatggcc ctggctaaac
     6421 tgacgcgttt tttcataccg ccggataaat cattgggcat catatcttca atcccatcga
     6481 gaccgacgag atggagtttt tccttgacga ttcgctgccg cgtcgccgca tcgtcgtgag
     6541 tatgctgacg aaggccgaag gctacgtttt caccaaccgt catggaatcg aagagggcgg
     6601 aatactgaaa aaccatcccc atgtgacggc gaacctccat cagctggtct tcatcataag
     6661 tggtaatatc cgtatcatgg aagaaaacat ggccccttgt cggcttttgc agaccgatga
     6721 tgaggcgcag gatcgtactc ttgccggaac cgctggcacc gaggacggcc aaggtctctc
     6781 cctgatgaat gtctaaatta atgttgtcca gtatgacctt atcgccataa gcgacggtca
     6841 catcgcgaag acgaatcatg atcctcctca atataataaa acggataaga tataattgca
     6901 gataaaaata aagatgatcg atgtcacgac ggatttcgtc gtcgcctgac cgacgccttc
     6961 agcaccactt tgagcattca tgccttcatt gcaggcaacg agggcaatga taccgccaaa
     7021 aaacatagct ttgaccatgc ccaggacgac atcgcttatg ttcgtaaata attcaatcga
     7081 atttacatac gtaaaatgcg tcagaccggc cccgagggtc gccacgccgt agccgccggc
     7141 cgtcccgatg acatagccgt aaaaagccag catcggcacc atgatgacgc a
//
LOCUS       DAWDXZ010000023         7132 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3332_length_7132_cov_7.924686, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000023 DAWDXZ010000000
VERSION     DAWDXZ010000023.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7132)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7132)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7132
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3332_length_7132_cov_7.924686"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    394..403
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2527..2536
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4470..4479
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1771 a         1545 c         1834 g         1952 t
ORIGIN      
        1 gtcgctggag ctacgggaga tgtattagaa gctgtcgttg ataaaatgat tgcggataaa
       61 aacatatctg tagaatatgc caaagacttg attacaagaa tgtaattaaa ggaggttccc
      121 gcaatgaaaa agacgtgttt ggctgacatc tggggtgata cggttgatct gtatgactta
      181 gggaagggaa tcgtcatagg aattgttata agcattagtt gttatttagg agcccatgaa
      241 ataattatta cacaggctcc ggaaatagca cctaaattag tttctgcata ttcgttacta
      301 tttgggattg gtggatgcgt tatttctgcc gtcgtatctg caaagttatt taatcccaaa
      361 cggacactga acgaagaaga attttctgaa aaannnnnnn nnnaaagtag tgcacgaaat
      421 gcaagacttg aaactatatg aattattcta tggacacgat aaaaataaat cgtcggaagc
      481 cttaaaagga gatgaaacat gatggagggt tttgttgtca gtgatttatt gtgggcttta
      541 gggatggcat tagctggcgg catcattttt tccggtgtcg gtttaatttc tggcacggat
      601 gaaacatcaa ccattgctcc tcttacccta ttaatcgttt tgttaggggt cccaccggta
      661 gccatttttt cctggtttat ggccgctgcg atttctaaac acatggtaca tgccatcccc
      721 acagctctaa tgggggtgcc gggagataca atggccgtcc ccatgcttga agatgcttct
      781 gttttgagga aactgggagt tccgcatatt gctcttcgta aaatgatatc tggcgggatt
      841 attgctgctt ttttagcctt gccaatcagt gtgggctttg cttctttatt agcacccttt
      901 gcagatattg ttaaagcgtg ggctcccgtt gtatttactg tagcagctat tattattgcc
      961 tatacttctc aaggcagatg ggccagtttg tttgctctga ttccgttcgc cttctttgtc
     1021 caagggttaa ataaaatggc tattgcaagt attgggcatg gcgtatttat tagtatgttt
     1081 cttgggattg cgattggtcc gatgtttgcg gatttaatga ccatattatt cccgggatcg
     1141 cgccatcttt tagcaagaag tcaggcacga gaattttggc ttgctccgga gttaaaaaca
     1201 tggaagggat actttcccaa tccagtgaaa attctatcta agcatcagat tttatacaat
     1261 gcgttgactg cggctgtttc agcacttaca tttacgttca gccctgttgg catgaccgtt
     1321 atgatggggg aatttgtagg taccagaata aaagggttat accaacggat gacgacaacg
     1381 ctggcagtta tgaatggcac gacagaagcc acttatattg cagaaacggt cattcctttg
     1441 cttgcttttg gtatcccact cagtcccgtt ggattgggac cagcagcgcc attgtttaat
     1501 gcaccgcctg ttttttcagc acagccggtt cataatttac ataatttttt aacccctgta
     1561 gattacttgg tttatggtta tattgggttg ttcgttgctt ttctggttgc ctaccctttt
     1621 tcgatgaact atgcacggaa agctagtgct tgggtattga aacatgttag ccaggaagct
     1681 attatcagca tgtttgccgg tttgattgtc gtaatatcgt actttgaagc aggcctggta
     1741 ggtatttcca ttgctgtgac tattgctgtg tttgggggta ttttgaacag atattttggt
     1801 gtccatacag gcgtgcagta tatggtctat tatgcctcgc catggcttat gctaaccttg
     1861 cttggaataa aataaccggt cgtataaagt tggttatttt aagaaaatca aagcggcatt
     1921 gatgatattg cattccatat catacttacg ggaacgctaa attatatttc acacataaaa
     1981 aggcgattta ctctgaaata acagagtgaa tcgccttttg tatgattaag tcttaaatat
     2041 agttaaatct ttaagcccac acgaagctgg ccatggagag agaccccatg gtgcaggtgt
     2101 tgttaaatac ggtggccgaa tcttctccgg cggcgccgag acagtaaagc agcggcatat
     2161 agtggtccgg tgtaggaacg gagtaggctg cgttttcgtg ttcttggtag ttgatgacgg
     2221 catcgatatt gccgttaagg acgttgtcta cgatgtattg gctaaagtgt tctgcctggt
     2281 cggaaccgcc gcggttgttc cattcgattc gccgcaggtt gtggacgatg ttgccgctgc
     2341 cccaaatcag gtatccctgc tcacgcaagg gggctagttt tctcccgatt tcgacacact
     2401 gttccgttct cagatggctg tttaccgaaa gctgtacgac ggggatagaa gcatcgggaa
     2461 acatatgaac catgacgctc caggttccgt gatcgatgcc ccagctgtcg ttgatagaaa
     2521 cgtcgcnnnn nnnnnncgct gcccaggcgg tcacagactt ctttcgtaag gtctgaacag
     2581 cctctgacgg gatatttaag ctggtagagt tcttctggga agccgtacat atcgtatact
     2641 tgacgtggtt tttcggcgct ttgaatatat gtacccggcg cataccaatg ggcggatatg
     2701 gctaatatgg cttttggctt ttccgggctg tttagaatgt cggcgccgat tcgctgcatt
     2761 tcgcgcgtaa gatcattttc atcgatggcc agcatggggc ttccgtgtcc gatgaagata
     2821 acaggcgtct ttttcatata acaaatcccc ctttgtcttg taattcgtaa atataaaccc
     2881 cttttagtat atcatataca tactttacgt caacaaaggg ccttgtttca tatattcaat
     2941 tgagagaagt gttttactga aaaacaagtg ccttttgaga atggccttat tgcccggaag
     3001 tccttatttt ggtatactac atagtaacgt acaggttacg gacttttttt agggataacg
     3061 actgtgcgtc cttttgggcg cccgagttat ctcttttttt tataggagga gttaccatgg
     3121 aaattcgtca ctatcgctgg aatgatggaa aaacgctgac tgtcggagaa agaacgctga
     3181 ttatgggggt cttaaattat acgcctgatt ctttttcgga tggaggcaag tggaatgata
     3241 tcgacgcggc cttacgccat atggaagaca tggttgccga tggggccgat attatcgata
     3301 tcggtgctga atcgtcccgc ccgggcttta cgccgatttc ggcggctgaa gaaattgaac
     3361 gattggaacg aatactgccc cgcctcgtcg atgcttgtcc ggtgcccgtt tctgtcgata
     3421 cgtataaagc cgaaacggca gactatgcca tgagcacggg agcccatatc atgaacgaca
     3481 tttggggcct gcagtatggg ccggaaccgg ggaaaatggc ttcagttgct gcaaaacacg
     3541 gcgtccccgt cgtcgtcatg cacaaccagg acagcacgga atacggcgac atcataggcg
     3601 atatgaagcg cttctttatt cgttcgtcca tgattgccga ccgggccggg atgcgtcaag
     3661 accaaatcat taccgatccg ggcatcggtt ttggcaagac ttttgaacag aacgtctatg
     3721 tcatgaagca tctgcgcgaa ttgacggtcc tgccgtatcc catgctgctg gggacgagcc
     3781 gcaagggctt tatcggaaag atcctcgacc tgcccgtaac ggagcggatg gaagggacgg
     3841 ggacgacctg tgtggccgga gtcctttcgg gatgtactat catgcgtgtt catgatgtaa
     3901 aacctattgt tcgtatgtgc cgcatggccg atgccttagt ggataacttt tgataaaaat
     3961 tgtattattt atgcatagat ggtgatctgc ttcttgaccg ctgtccattc tggaagtata
     4021 atagcccaaa tactgcggac gtcaaaagct gtgccgcaag ggggagatgg ttctatgaag
     4081 tgtgaaagtt tacgaggcct cgtcgtttgc cgggattttt tgaaggatcc tgtcatcaag
     4141 gccttgatgg atgttcagga agatacggat caagaggctt cgttttcacg gcgagccggt
     4201 gcggccgcct tattggaagc ggctgaacgg ttggggcttc agggcaatgt gctgcgccag
     4261 tattttttat accttttttc tgagggacgt acggtagctg ccgatgttat ggaacgggtc
     4321 ggtaccgcag gtacgggact gactcgggct ttggctttcg atatgaaact gatatttccg
     4381 tacttacagc gtcagccttc tgacttcttt gatatcgaat atttagatga ttatgagccg
     4441 gcttccgtca caaacgacgc ctgcattcgn nnnnnnnnnc tgcattcggt ccttagagaa
     4501 agcttttcaa ggggcggata cgccggaaaa agccacggcc gtcctgctcg attactatgc
     4561 cgccttcggc cgcgggaaac ttgctcgttt catggccttt cggttgtctg atgacggcca
     4621 gctcatcggg attgaaaatt tcccgcacct cgattggaat gatctcattg gctatgctgc
     4681 ccagaaggaa aaactcatgg ccaatacagc ggcttttgtc agcgggaaag aagcgaataa
     4741 cgttctcctt acgggatctc gcggtacggg caagtcgacg gcagtcaaag ccttggtctc
     4801 acgatatcat gaacaaggac tgcgcctggt tcagcttcag cgtgaccagc ttcagggact
     4861 acagcccctg atggaaaagt tgggcgctat cggcagcaag cgtttcatct tgtttctcga
     4921 tgacctgtct tttgatgaaa atgaagcgtc ttataagtac ctgaaatcgg ccatggatgg
     4981 tggtgttacg ccacagccca aaaatgtggt tatttatgca acttccaatc gccgtcatct
     5041 gctcaaggag acctggcatg accgcaatga tgaatgggaa gaagtctacc gtgacgataa
     5101 caccaatgaa gccatttctt tatcagaccg tttcggcctc atccttcatt acgcagcgcc
     5161 gacgcaggat gaatatctgg aaatcattgg ccatgaattg aaaaaagccg gcattgatct
     5221 cgctccggaa aagctgcgaa tcgaagggct gcgctgggag atggaacatt ccggccgcaa
     5281 cgggcggatt gcccagcaat ttgtgaaatg gtacctcaac aatcgggact cctaaaaata
     5341 ttaggaagtc aagcgtttaa ctaaagaatt tcaaattaat ttcaaaaaaa tcattgacaa
     5401 acgaggcgtc attttgtaga atgtgttcaa gcttttcaaa cgagccgaac tgataagggc
     5461 tcgcaaaagc atagagtaaa atctgatgac gggaaagagt aggtccttcg gatactttca
     5521 gagagtcggc gggtggtgcg agccgatagt gaagatcgat tgaaaatcat ccctgaagag
     5581 gagtgctgaa gtatactaag cactgacgac ttttccacgt tataggaaac gcgtatgttg
     5641 gtacgttgta gagagcctgg cgcagcaatg agccgggaat cagggtggta tcgcggaata
     5701 aaaactccgt ccctcatagg gataggagtt tttttgttac ctctttcgac gcatctctaa
     5761 attccaacga gaataaatta ttttatactc aaaggagcga tgttattatg gcaaaggttt
     5821 tttatgatca agatgtaaat tgggatgtca tgagaggtaa gaaggtagct gtcgtcggtt
     5881 atggcagcca gggtcatgcc catgccttga atttgaaaga aagcggcgta gacgtcgtcg
     5941 tcggtttgta cgaaggcagt aaatctgtcg aaaaagcaaa aaaagccggc cttgaagtca
     6001 agaccgttgc cgaagccgtt aaagaagcgg acgttaccat ggtcctcatt ccggacgaaa
     6061 aacaggcgga tgtctatgct tctgaaatcg ctccgaattt aaaatccggc agtgcccttg
     6121 cttttgctca cggctttaac gtgcacttca aacagattgt cccgccggct gacgtcgatg
     6181 tcttcatggt agctccgaag ggacccggtc acctcgttcg ccgtaccttt gtagaaggcg
     6241 gcggcgttcc ggacgtattc gctgttgaac aggatgcgac gggaacttgt tttgatttag
     6301 ccctggctta tgcccgcggt atcggcggaa cgcgtgccgg cgtcatccag acgacgttcc
     6361 aggaagaaac agaaacggac ctcttcggtg aacaggccgt tctctgcggc gggatctgcc
     6421 agctcatcac caacggtttt gaaacgctct gcgaagcagg gtatcagccg gaaatcgctt
     6481 actttgaaac cttccatgaa atgaaattga tcgtcgacct catgtatgaa ggcggcatgg
     6541 ccaagatgcg taaatccatc agcgatacgg cggaatatgg cgattatacg gccggccccc
     6601 gcgtcgtaac ccaggcttcg aagcaggcca tgaaagacgt cctcaacgac atccagtccg
     6661 gtgcctttgc caaagattgg ctcctcgaaa acaaggccgg cggccgggct cacttcttag
     6721 ctatgcgccg tcagcacgcg gaacatcctc tcgaacaggt cggtgaaaag cttcgcgcca
     6781 tgatgccctg gctccacagc aacgatcaga acgactaatt tcttcatggt ccatagacca
     6841 tgctcttaat gacatgataa cgaggtgaat agaatgaaca tgaccggcgc acaagctgtc
     6901 cttgaatgct taaagcgaga aggtgtagat gtcgtattcg gctatcccgg cggagccgtc
     6961 ctaaccttat acgacgaatt atataaagcg cagttccctc atattttgac gggacatgaa
     7021 cagggagctg ttcacgctgc cgacggttat gctcgagtga cggggcgcac cggagtctgc
     7081 tttgcaactt ccggcccggg agtctgcaac atggtgaccg gcattgcgac gg
//
LOCUS       DAWDXZ010000024         7000 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3374_length_7000_cov_9.624910, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000024 DAWDXZ010000000
VERSION     DAWDXZ010000024.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3374_length_7000_cov_9.624910"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1290..1359
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1452 a         1694 c         1988 g         1796 t
ORIGIN      
        1 tgcggtgacg gcgaaagaaa cgccggtcct caagaaggcc gtccgttggc ttgacgacta
       61 ttttgccggt cgcatgccgg ctatcatgag ccttccacta gcgccggagg gtaatgaatt
      121 ccgccgccgc gtatggagcc tcctctgtca gattccctat gggcagacga ctacctatgg
      181 cgagttggcc aaagccgtgg aagcccagac ggggaagacg atgtctccgc aggccgttgg
      241 cggcgctgtc ggtcacaatc ctatttcggt tatcattccc tgccatcgcg tcttaggggc
      301 caggggtaag gcaacgggat atgccggcgg catcgataaa aaaatagccc tccttcattt
      361 ggagggcttg cttgtcaaag aatgatgtct tacgtttttg tcagggcttc gatgaattga
      421 ccggcaatac tggacaggta gcgatgtttg gcggtgatga cggccaggcg cgtgcagagc
      481 cgtggttcgt cgaggacttt catttggagc gtcgtcagcc ttgacggtga gagggctgag
      541 gccgggacaa cggcatagcc taatccggct tcggcccaaa ggagtgtcgt ccgggcatca
      601 tcgtttcgac agaggatgga cggtgtaagg ttgtgtcctt cgcagacgtc gaggaggagc
      661 tgattgaatc ggcggtagac gatgaggggc aggccgtcta aatcagggat ggtcaaggtc
      721 tctttttcgg gggccggatc ttttccggca taggccagca ccatcggttc ttccggcaaa
      781 aaggtacagt cgaaggcttc ctgcttaaaa ggcgtgcgga cgatgccgat ttcaatcagg
      841 ccctttcgca gcatatctaa gatgtggaag gtattgccgt catagatttc aaagcggatg
      901 ccgcggtggc tgtcgtgaaa ttgccgaagg gccggtcgca ggatgatact gcctgaggac
      961 gagacggtgc cgatggataa ggtaccgcgc agtccgcttt taaagtcgcc gatttcccgg
     1021 cgtgtcgagt cggccagggt gatgagctgg cgggcccgtc ggtagaggat ttctccggca
     1081 tcggtcaagg tgacgctgcg cggttcccgc tggaagagtt cgacgcccag ttcttcttcg
     1141 agggctttca tttgctgact caatggcggc tgagccatat tcaggcgccg ggcggcggcc
     1201 gtgatttggc cttcttcggc gatggccagg aagtatgtga gttgacgaag gtccatagtc
     1261 tctccttttt tgtggcatat gatttacgtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc aatatagata tacctatggt
     1381 atactagcca tgtaaaaaaa tccctttaat tttcagaaag gatgaatctc atggatcttg
     1441 tcacaccgaa acgagacgcc catttttatt ggacgctctt caaatcgact tttttaatca
     1501 gtgccttcac cgtcggcggc ggcttcgtca tcattccctt gctcaagggc aaatacgttg
     1561 acgaatacca ttggatcagc gataaggata ccctcgatat ggtggccatt gcccagtcta
     1621 tgccgggcgt cgtcgccgtc aacagtgccg tcatcctcgg ctaccgcatg gcaggggttc
     1681 tcggaacctt ggtggccctt gtcgccacgg tcctgccttg tttgattacg ttgaccatta
     1741 tttcttacag ctatgattat tttattcaaa atgcctatat taaaatggtt ctccgcggca
     1801 tgcagtgcgg ggcaacggcc ctcatcgtta atgtcggcat cgatcttttg gtcaaacaag
     1861 gaaaaaagaa gctgatacta ccgatgctga tcatcatcgg aacctttgtg gccaacttgt
     1921 tcttcgatac caatatcatg gtgctcatcc tcatcgacgg catcatcggt ttcttgttca
     1981 tgcgcgatgc gcagtataat tagggggtgc cgtcatgatt tatgctttgc tattttggga
     2041 attttttaaa gtcggcatct tctgtgtcgg tggcggctat gcctctatgc cccttattca
     2101 ggccagcgtc gtcgatacct tccattggct gaccatgagt gaattcgtcg atatctttac
     2161 gatttcgcag atgacgccgg ggccgatcgg cataaacgcg gcgaccttcg tcgggacccg
     2221 catcgcaggt cttggcggtg ccgtcgtagc gacgctgggc tttgtaacgc cgtccttttt
     2281 cttgggtatc tttttagccc gcatcttttt gaagtacggc gatatcggac cgattcgcgg
     2341 tatcttgaac ggcctgcgtc cggccgtcgt agctcttatc tgcgtagcca gcctgagctt
     2401 catcctcttg gctttatgga atacggaaga gctgccctct gattggctgg ccttcagcag
     2461 cggcggactc gtcatcctca tcgccgctat cattgcaacc cgtaagggaa tcggcgtcat
     2521 caaggtcctc ctcggttccg gcgtcttagg tctggtactg gggatggccg gccttatcta
     2581 attttatagt aatcatcggt gataccatgc aggaatcggc tcggctgata gctctgccgg
     2641 gcataccctt ggtgccagct cagatacagc ctcttttttg cacgcgtcat ggcgacataa
     2701 aagaggcgta tttcttcttg tatacttcca ttttgtcggg accaataggt cgggaagaca
     2761 tggtcttcta aactggccat aaagacatag tcgaattcaa ggcctttggc ttggtgaatg
     2821 gtgatgatcg gtattttcgg gtctttggac agcatgctgt cgagttcgct gttggaaagg
     2881 gccgtcatct tgagaaattc ggtcagggcg tcgcgcggat tctggccggg atcgtccgtt
     2941 tctttggcga tgcggtagag cttgcggatg ttttccagcc ggtcgatgtg attggctgca
     3001 tcggaggtat cgcgatgtgt ttgataccag gctttgacgc cgctgtcgtt catgacggac
     3061 gtaatgactt cggtgggccg ggccttatat gacaagcgcc gcaaggcgtc catttcacgg
     3121 ctgaaggggg caaagagggg gagatagatg gccatcgcgg gtcgccctgc cccttccgtc
     3181 gggcggatct tctcgtcgat ggcatagacc aggagcccgg ctgtggccag aatgtcgtca
     3241 aaggcgtcgt gcgtcggttt atggccaatg ggaaagtggt ggctcaaggt gtctaattta
     3301 tggttaatca agttcggata aaaacggcgg tagatgtcga gggtatcgta atagggaaag
     3361 tctgcccttt cggaaatgcc caggcgctgg agttcgctgt ggaggatgga caagtcatag
     3421 gtgacgttgt ggccgacgat gacggcgtct ttggcaaagg ccaagaactt tgtcagggct
     3481 tcttgcggcg gaattccttc ggcggccagc ttttcgtcgc tgatgtggtg gacgtagtat
     3541 gccgtgccga cggagcggcc ggcctttaca taggtgttga acttatcctt tacctgcccc
     3601 ttggcatcga ggcggatggc ggcgatttgg atgatttcat cctgcgtcgt gtccgtaccc
     3661 gtactttcga cgtcgaagac gaccagccgt tcctcggcta aggccgtcag caacagccca
     3721 taagggtcgc ccgcttggtg ggacagggga tcgatgaaat cgctgagcat gaggccgacc
     3781 tttcgatagg aatcgctttc aatggtctcc aatgtacgcc ggccgatgcg ggggacgaag
     3841 cgttggacga ttcgcttcag gctgaggttg tcgtgcttat tgagcgagag tctaaggtag
     3901 gccaggacgt ccttacattc ctgccgtttg aacaagtgaa actggtcgat gcaggtaacc
     3961 ggcagctgct ggttttgggg gagcttttgg ttgtgcgagg taatgcccat ccagatcagc
     4021 ttagcctggc ggttgtcgcg gacgagaatg caggtcttca gttgttcttc cggtggcagg
     4081 gccttgattt cccggaaaat ccagcgggct tcctccatcc aggtgtcggc tttatgaagg
     4141 acgatgggtt cgccgcgttc ttttgacgcg gagatgcccg gccgggtata gattttctgg
     4201 acggtcgcca gccccatgcc gcggaataag aacgtctgag cggcatccag caggagttgg
     4261 gtagatcggt aattttggat gaaggtaatt tcccggggat gataaccggc tctaaattct
     4321 ttccaaatgt attcgggata ggagccgcgc cattcgtaaa tggtttggaa gtagtcgccg
     4381 caaaggagga tgttgcggcc cgggaagaga cggctgatga cggtgtactc gacttcgctc
     4441 gtatcctgca tttcgtcgac cgagatgaag cggaactttt cccgccagcg gcggcagatg
     4501 gcatcgtctt ggagaaattc ataggcaccg gtaatgaggt cggtaaaatc gatggcgtgg
     4561 ttttcggcaa gggcggcgtc gtactcccag acaaggtctt cgccgtagtc cgcgagccac
     4621 cctttgaggt catcctgaat attggtaaaa tcgcgcaggc cggggaagac tcgtttcttt
     4681 ttttcaaatt cgatgaaggt ctgaagttcc tgtaatgccg gacgcggtgt cgtttgtgta
     4741 agctctatgg cgccgataat ctgtttacag tcttcatcgt cataaatcag gaaatcgttg
     4801 tagcggtcgc tttgccgctt ggtctcttct ttgatgaggc tgtagcaaaa gctgtggatg
     4861 gtctttacgg tgacggcgaa cccttcttcc ggaactgttt cgatgatacg gttcttcagt
     4921 tccttgcagg cccgattggt aaaggtcagg cataagattt ccgaaccttc ggctttcttt
     4981 ttttctatga tattggcaac cctcgctgcc aggacccggg tcttgccggt cccggccggg
     5041 gcattgagta aaatggactc tgacagctct tgtacgactt gctgctgctc ggcgtttaaa
     5101 ttcatggata ttcctccgtt cgatcgcgtc tttgctttat tgtaacacag tccccgatag
     5161 ggggatactg tcttaccata gaaaggcggt ttttcattga caggcaggtg tgtttttgat
     5221 agaatcaaag tgtgtttgat aaatatttta atcaataagg agacgcacta tgacaaaatg
     5281 tttatggaca ggtttttatg ccgtttgtaa acagtctccg tgtatctaac tgaaagccca
     5341 aaggtgattt ctgtcacttt tgggttttgt tattcctaag gaggggtctc tgatgaaggt
     5401 agctttaatt atgggaagcg actctgattt tcccgttatg aaaaaagcag tagctatttt
     5461 taaagaattt ggggtagact atgaagtctt attggcatcg gcccatcgga cgccggcagc
     5521 ggttcgtgaa ttcgtcacga cagctgaaga acgcggtatc ggggcttttg tcgtcggcgc
     5581 cggtatggca gctcatttgc ccggtgtcgt agccagctac acgacgctgc cggtcatcgg
     5641 cgttcctttg gaaagcggca gtttgcaggg catggatgcc ctcttggcca tcgttcagat
     5701 gccgtcgggg attcccgttg cgactgtcgc cattaacggc gcaaagaatg cggccctttt
     5761 ggctgttcag atcggcgctg tcagcgatgc cgaattagcg aagaagtaca aggcttatcg
     5821 cgaaaacatg gccaatgaag tcatggctaa gaaccagaaa ttacaagaag acgtaaaagc
     5881 cctgtaaggg gattgcgtcc tatttttctc aataacagga ggtttaattc atgttttacg
     5941 atccgatttg ggataagcag catgaagaat gtggcgtctt agggattttt gataaacacc
     6001 ttgatattcc ccgctacgta tattgggggc tctttgccct gcagcaccgc ggccaggaaa
     6061 gcggcggcat tgccttgact gacggccagg atatccatac ctaccgcggc atggggctca
     6121 tcagcacggt ctttgccgac ggcgtaccgg aagaagaagg ggggcctgtc ggtatcggcc
     6181 acgtccgcta ctcgacgacc ggttccaata acccgcgcaa tatccagccc ttggcggtat
     6241 atacaccgat gggacagttg gctctggctc ataacggcaa cctgaccaat acccgggctc
     6301 tccgcgaaga actcgaccgc ggcggcgctg ctttccagac gactatggat agtgaaatca
     6361 tcgtcaacct catcagccgc agccagaaat ctaccattga agaacgtatc atggaaagca
     6421 tgaaccgcat tcaaggtgcc tactctttgg ttctcatgac caaagacacc ctttatggcg
     6481 tccgtgaccc ttatggtttc cgtccgttgt gcatcggccg taccgatgaa ggcggttggg
     6541 tcctggcttc ggaaacgtgt gccctcgacg ccattggcgc taaattcgtc cgcgacgtcc
     6601 atcccggcga attcgttcag atcaatgaaa acggggtcaa atcgacgatc ttcgccaagg
     6661 caccgcgcaa gcaggtatgt tcttttgaat acatctattt tgcccgtccc gacagcgtca
     6721 tcgacggtca ggacgtctat caggcccgcc tgaacatggg tcgtgaaatg tggaatgaaa
     6781 cccagtacga tgcggatatc gtcatcgctg tccccgacag cggcaatacg gcagccatcg
     6841 gctactccca ggcttcgggc attcctttca aacatggtct catcaagaac cgctacatgg
     6901 gccgtacctt tattaagccg aatcagaaac agcgtgaatt agctgtccgc atgaaactca
     6961 acgtcgttaa atccgtcgta aagggcaagc gcatcgtcat
//
LOCUS       DAWDXZ010000025         6867 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3431_length_6867_cov_8.629477, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000025 DAWDXZ010000000
VERSION     DAWDXZ010000025.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6867)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6867)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6867
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3431_length_6867_cov_8.629477"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    466..475
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4133..4142
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5425..5434
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6000..6009
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6536..6545
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1544 a         1915 c         1883 g         1475 t
ORIGIN      
        1 gcgctgtaag aaggggacct tccagaaaag gaccgggccc tacggcgcct tttgggtctg
       61 cagcggagcg acgtgcaaca ccaagtgtgc cgacgtcgac ggcgtgccca gcctgtacgc
      121 caagtcctga tgacggccat atgtcaaaag gacggatata caaaagggcc gccctatggt
      181 attataaaga ttaagctatt ttttttcata gctttttgtc catataggag taagaatcat
      241 aaatgaaacg catttattta gataatgccg cagcgacgcc tatggatcgg gacgtcctgg
      301 acgccatgat gccgtatctg acgacatctt acggcaatcc gtcgagcctc cattactttg
      361 gtcaacaggc tcgggctgcc gttcagaagg cacgcggtca agtcgcacac tgcctcggtg
      421 tcagtggtga agacctggtc tttaccagcg gcggaacgga agcggnnnnn nnnnnacgtc
      481 cccgtcgatg gagacggctg cgtctcagca gacgatgtgg aagctgccat cagggacgat
      541 acggccctca tttcggtcat gtacgctaac aatgaaacgg gcatgatcca gcccattgct
      601 gccatcggag cagtagccaa ggaaaaaggc gttgcctttc atgtcgatgc cgttcaggcc
      661 ttcggctatc tgcccgtccg gcctatggaa gaaaatatcg acatgctcac cgtctgcagc
      721 cataagattt atggtcccaa aggcgtcggt gccttgtata tccgccacgg actcaaggtg
      781 acagccgaag cctacggcgg tcctcaggaa caccgcctgc gggcagggac cgaaaacgtc
      841 gccgccatcg tcggtttcgg cgaggcagcg gccttacttg aaaaagaacg ggacgatcgg
      901 gccaaagccg cccgggtttt gaccgacatc gtctatgaca agctcattca aggcgacgat
      961 cggttccgct taaacggcaa tcgccgtcag gttctgccca atatcatcga cttcagcgtc
     1021 gacggggtgg acagtgccat tttactcatc gccctcgatt tacagggcat tgccgtatcg
     1081 gccgggtcgg cctgcgaagc cggtgccgtc gaagcgtccc acgtcctaca ggccatgggc
     1141 gtcgaagaaa agtggctgcg cagcagcatc cgcctgtccg tcggcaaaga caatacgaca
     1201 gaagatattg aacacgccgt gtcccggatc cgggacgccg tgacgaccag tagaggagat
     1261 tagacatgaa acgagtcgcc gtagctatgt gcggcggcgt cgacagctcg gttacagccg
     1321 gtcttttaaa gcagcaaggg tatgacgtca tcggcatcac cctgcgcctg tgggaagaag
     1381 acccggcctg caccatcgac aacgtccacg cctgctgttc cctcagttcc gtcgacgacg
     1441 ccaaagccgt cgcttcggtt ctgggcattc cccattatac cttggatttc cgcgaactgt
     1501 tccgccgcga tgtcatcgac tatttcgtcg attcctatcg ccggggcaag acgcccaatc
     1561 cctgcattgc ctgcaataaa tttattaaat tcggcctgct ctgggaaaaa gccaagcagt
     1621 ttggcgccga tttcctggcc accggccatt acgcccgtct ggcaaaagac gaacagacgg
     1681 gaatctattc gctcttacgc ggtatagacc gccgcaaaga tcagtcctac gtcctctatc
     1741 agctgaccca ggacatcttg ccgcacctcc tgttccccat ggccgatctc gagaaggccg
     1801 acacgcggaa actggccaaa gagtggaacc ttccggtctt caacaagccc gaaagtcagg
     1861 atatttgctt cattcccgac aacgactaca agggcttttt ggaaaaggaa cagaaggata
     1921 tctttaagcc cggcgacttc gtcgatgaat cgggccgcgt catcggccgt catcagggca
     1981 ttgccgccta taccgtcggc cagcggcggg gactcaacct cggcggtccc ggcggtccct
     2041 actacgtcac ggccttagac gtcgaacata atcgggtcat cgtcggttcg gaaaaagacc
     2101 tgttttcaaa gactgtcgaa gccagcgaag cgacgtgggt cgaacaggtg ccgacagcgc
     2161 ccttccgggc cctcggcaag atacgctatg cggctaacga atcgccctgc acggtctatc
     2221 ctgacggcga cgacatgcgc gtcgagttcg atgaagccca gcgtgccgtt acggccggcc
     2281 aggccctcgt gctctacgat gaagaaacag gggaacgcgt cctcggcggc ggcgtcatcc
     2341 aataagaaag aggggaaaca tacggtgtcc acagatcata tcagaaactt ttcaatcatt
     2401 gctcatatcg accacggcaa gtctaccttg gccgaccgga tgctggaaat gaccggcact
     2461 gtgcccgaac gggaaatgga agcccagtta ctcgacacga tggaccttga acgagaacga
     2521 ggcattacta tcaaagcgca gtcggcccgc ctcatttatg atgccaaaga cggcaaacag
     2581 tatacgctga acctcatcga tacgccgggg cacgtcgact tcaactacga agtatcgcga
     2641 agcctggccg cctgcgaagg ggccctgctc atcgtcgacg ccacccaggg cgtcgaagcc
     2701 caaaccttgg ccaatgtgta ccttgccttg gaacatgact tagaaatcat ccccatcatc
     2761 aataaggtcg acctgcccag tgcagacccg gaccgagtct gcaaggaaat cgaagacgtc
     2821 atcggtctcg atgcctccga cgctatcatg atcagcgcca agacgggcct cggcgtcgac
     2881 caggtcctgg aagccattgt ccagcgcatt cccgccccgg ccgacgaaga agataagccc
     2941 ttgcgggccc tcatcttcga ctcccacttc gatgcctata agggggctat cgctaacatc
     3001 cgcatcatgg aaggcagctt aaagccgggc cagcggatcc gcatgatggc ttcgggcaaa
     3061 gaatttgaag tcatcgaaac gggcgtcttc ctgcctcaga tgcatcccgt gccgtctctc
     3121 ggctgcggca gcgtcggtta tttggcagcc agcatgaaga acgtccgcga ctgccgcgtc
     3181 ggcgacacgg ttaccttggc cgaacggccg gcgtcggaag cattgccggg ctatcgacgg
     3241 gccgtgccca tggtctactg cggcctctac ccggtcgaaa cgaatgatta cgataatttg
     3301 cgcgacgcct tggaaaaact ccatctcaac gacgcggccc tgctctttga accggaaacg
     3361 tcgtcggcct taggcttcgg cttccgctgc ggcttcttgg gcctcctcca catggacgtc
     3421 gtccgcgaac ggttggaacg ggaatacgac ctgtccctga ttacgacggc gccgtcggtt
     3481 atttatcgcg tctactgcac ggacgggacg atggtcgaag tcgacaaccc gtcgcagatg
     3541 cccgatatga ccaaggtcga ccacatcgaa gaacccttgg tcaagacgac gatcatcatc
     3601 cccaaggaca tggtcggtac ggtcatggaa atttcccaga gccgccgcgg ccagtacgtc
     3661 accatggact acctcgacga tacgcgggtc tccttggtct ataacctgcc tttgtgcgaa
     3721 atcctttacg actacttcga cgtcttgaag tcggcgaccc gcggctatgc gtccctcgac
     3781 tatgagctca acggctacca gtcgtcgcct atggtcaaga tggacatcct cattaacggc
     3841 gacgtcgtcg acgccttgag cgtcatcgtc caccgcgact ttgccgcccg ccgcggccgg
     3901 gccctggtcg aaaagctgcg cagcctcatt ccgcgccagc tcttccagat ccccgtccag
     3961 gccgctgtcg gcaataagat catcgcccgc gaaaatgtag cggccctgcg taaagacgtt
     4021 ttggccaaat gctacggcgg cgatatttcc cgtaagcgca agctccttga aaaacagaag
     4081 gaaggcaaga agcggatgaa acagttcggc accgtcgaaa ttcctcagga agnnnnnnnn
     4141 nntatacgtt cacattccct tttgccggca taaatgtttg tactgcgatt ttccctccta
     4201 cagcggcctc gaacgctatc tggaaagata tgttccggcc ctctgccggg aaattgcggc
     4261 cacgccctat gccgggaagt ccgtcgatac gatttatatc ggcggcggta cgccgtccat
     4321 tctttccgca aaggccgtgg gagacatcct tcggcagctg cggcagacct tcgaggttac
     4381 ggcggaggct gaaattacga tcgaagccaa tccggaaagc ctcgacgggg aaaaggcggc
     4441 agcctacgtt tcctacggca tcaaccgttt gagtctcggc atccaatctt tttccgatga
     4501 gatgctgtcc tttttgggcc gcattcacac ggcaaaggaa ggggagcggg cccttcgtgc
     4561 cgcctgggac gccggcatcc gcaacctgtc tatcgatctg atgtacggcc tgccgggcca
     4621 aaccgtcgag gacgtccggc gcgatgttca gcgcttggga gaccttcccg tggaccacgc
     4681 ctcgatttac agcctcatcg tcgaagacgg gacgcctttg aaacacggtc tttctaaggg
     4741 cctttggaca cttcccgatg acgaggttgt cgaagccatg ggccaggccg tccacgatca
     4801 gatgcacgac ctgggattcc accactatga aatttcaagt tatgcaaaag ggccttttga
     4861 atcgaaacac aacagcaagt actggatcta tgagccctac atcggctttg gcgtgtcagc
     4921 ccactccttt gacggtacgg cccgctgggc caacatggcc aatatccctc agtatatcga
     4981 aagggccgga aaggggagcg tcgaaggcga acgcatcctt atcgacgcta agcgagccgt
     5041 cgaggactac tgcttcttag ccctgcgccg ccgcgtcggc atcgataaag aagactatga
     5101 ccgccgtttc gggcagccca tcgaagccga tttcggtccg gtcatcgatt cgctcgtcgc
     5161 tcagggcctt ttggaaaata cgaaagaatg ggtccgccta agcgatagag gactttctta
     5221 cggcaactac gtctttggca agttcatccg ctgaactttt tacgcggata atccccaacc
     5281 cgggataagc aaaaaaatga accgtcagga atcgacgttc ctgacggttt ttctgtatct
     5341 tctctgaggt cggttcgacg ctcattcgct tcggcccgga tctgtgcttc ggctttcgcc
     5401 gtgttttttt cggtattctc gaggnnnnnn nnnngcaaag tggctctgac tgtcgaagcc
     5461 gcaggcggcg gcgatggcgg caatgctctt agtcggcttc gtcaagagct ggtcagcgcc
     5521 ctgcatgatc cggtatttta agacgtattg gatgggcgac aactggatcg tcttttgaaa
     5581 gcagcgcagg cattcacgct ggctgattgc ggcggcgtcg gcaatatcgg tgacggtgag
     5641 gggttcttca aagtgggtgt ggatataagt caacatcttt ttgatccgtt cttcgtcaag
     5701 actttgcatc tctgaagggg catctccgtc gggacggaat tgatcgtaca gaaagagaca
     5761 gatttgtgac agttcttctc ggacggtaaa ttcaaagcct gacggttcgt ccgtcatggc
     5821 tttaaaagcg gtgcggaagc ggcgagctgt cgtgtcgtct tggcttcccc ggcagtaaaa
     5881 ggccgaaaag gccggacagg tgaggagggg acgaatatac ttcacccata gaatcgagtc
     5941 gtcactgccg gaaatccgcg acggacagaa gacaccggag cgcaggtcgc attccggatn
     6001 nnnnnnnnng gccgcatgaa gggtattgcc gttgacggcc aatatatcgc cggcggtcag
     6061 atggaacgtg tgagccggga cctttgcggt cattttcccg cctgcgatgt agatgatttc
     6121 cagttcatcg tgccagtgcc aggggacgtt atcgcctatt tcctgctggt aatggacggt
     6181 gtagccggca caggggaagg ctgcgtttcc gtggggtttt atttcttggg atcggtcgtc
     6241 gaccgggata gaacagtctg tcaatatcat ggggacacct ctttatttgg ctgttttatt
     6301 atagtatacg gctgaatttt cattgtatca agacctatat gacggtattc ttataataga
     6361 gaagggaaga aaccacacgg ccgcgatgcg gcgaaggagg tcttgatgat ggtattaaaa
     6421 ttttacttca ttcccaacat ggaaaccttt acggctctcg tcgccgccag ccggggatcg
     6481 gtcctggtcc ggcgggatga agcgtttcaa agccttaaag gcgatggcga cgcctnnnnn
     6541 nnnnnccttg tcctttataa aggagagaac cgcgcggggc cgcggcgtag aaattcgttt
     6601 gagtgatacg cgggattact tttccttcgt ccgcttcatg gtcggggctt gtctttacaa
     6661 ttgacagaat atacgtgccg taacggataa atgtacgatg aaaaaaaaca atttccatag
     6721 cggaaagttt tattcgatcc ggggccgatg caggtgaaag ccgactccaa gggcgttgac
     6781 aaagttctga taaatcgtat cagaacctca taagttattg acaaacagcc ataatctgag
     6841 tataatttca ttgtgtgcag taacagc
//
LOCUS       DAWDXZ010000026         6774 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3475_length_6774_cov_9.222801, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000026 DAWDXZ010000000
VERSION     DAWDXZ010000026.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6774)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6774
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3475_length_6774_cov_9.222801"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    956..965
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5135..5144
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1451 a         2012 c         1813 g         1478 t
ORIGIN      
        1 ccgtcacagc cggtaagcag gttgccgtct tggtaccgac gacggtcttg tctcagcagc
       61 acttccagac ctttatggaa cggttcggcc ccttcggtgt ccacgtcgaa gtcctcaacc
      121 gtttccgttc ctacaaagaa aagaaatcga tcctggcccg gaccttgacc ggcgatatcg
      181 acatcctcat cgggactcac agcctgctca acaagaacgt taaattcaag tccctgggcc
      241 ttctcgtcgt cgacgaagaa cagcgtttcg gcgtcgccca aaaagaaaaa tggaaggcct
      301 gggccgccca tatcgacgtc ttgtccctca gtgctacgcc gattccgcgg accctccaca
      361 tgtctttggt caacctgcgg gaaatgtcgg tcatcgaatc gccgccgacg gaccgcttcc
      421 cggtccagac ttacgtcgct gaatacgacg cccgcatcgt cagtgacgct atcatgcgcg
      481 aaaagaggcg gggcggccag gtctttttcg tctataatcg tgtcgcttcc atcgaacgca
      541 tgaaggacga actgcaggcc ctcctgcctg acgtaaccat cggcatcgcc cacggtcaga
      601 tggccggcag catgctcgag tcgatgatgt tcgatttcta tgaaggaaag tacgacgtac
      661 tcctctgctc gagcctcgtc gaaaacggcc ttgacgtagc caatgccaat acgatcatca
      721 tttacgacgc cgaccacttc ggcctgtctc agctctatca gatgcggggc cgcgtcggtc
      781 ggagccaccg catggcctat gcatacttct tgtaccgcag ggacaaggtt ttgtctgaag
      841 tcgctgaaaa acggctccaa gccatcaaag aatttaccga actcggttcc ggttttaaaa
      901 tcgccatgcg cgaccttgaa atccgcggtg ccggcaacct cctcggtcgg gaacannnnn
      961 nnnnngtcgt ctcttcgaaa gaagagctgg acgaactgcg ccgcgaactg gaagaccgct
     1021 atggccgacc cagttcgtac gtcgaaaagc tccttcaggt gacggaaacc cgtctcgaag
     1081 ccaaggctca gggaatggtc ctcatcgccc agaagaagga cgtcctcgaa atcaagtggc
     1141 gccgcgtctc ggacatgccc gatttgacga aactcgaaaa gcccctgcgc agccgcttcc
     1201 gcctcgttcc cagtataccg gtcatggtcc gcgtatccct ttccggtatc gccgatatcc
     1261 tcgactttat ccagaccttg ctgacggcct tttcctccct tcgaaaggag gattcccgtg
     1321 actaactttt tacgcggcgc catggtcctc accgtagccg gcatcgtcgt caagatctta
     1381 ggcgggttga accgcatttt cttatcgcgg ctcttaggcg gcgagggaat cggcctctat
     1441 cagatggcct atccggtcta catcctgctc ctcagcatca tcggcgccgg tattcccatt
     1501 gccgtgtcga tcatgattgc cgaacaagcg gcccgaggcc attacagcgg cgtccgccgc
     1561 atctttcacg tgaccttggc ctttatgacc gtcgtcgccg tcctctgcgg cctggccctg
     1621 ccttttggag cccaggctat gatcgagttc ggcatcgtcc gcgacagtcg ggccctgccg
     1681 gccttagctg tgttggcacc ggccttaagc ttatcggtca tcgtctgctg cttccgcggc
     1741 tatttccaag gcctgcagct catgacgccg acggctctgt cccagatggc cgatcaattc
     1801 gtccgcgtct gcaccatgct cgttctcgcc tggattttcc tgcccagagg tcttgagtgg
     1861 gcggcggccg gtgcttcttt cggtgccgtt cccggggcag ctgcagggct catcctcatc
     1921 gccttcttat actaccgtca tcaacggaat ccgttacccg atgacgacgg cgtcttcgtc
     1981 atccagtcgg cgcggcacat catccgccgc ctcgtcgtct tagccgttcc cgtagcggca
     2041 gccaacatgc tccttccggc cgtagccagc atcgatctct ttatcgtgcc tctgcggctg
     2101 gaagcggccg gttacagtac ccatgaggcg acagccctct atggctacct gacgggcatg
     2161 gccaacggcc tcgtccaatt gccggcaatc ttgacgattt ccttggcgac cagcttagta
     2221 ccggccgtct cagcggcctt ttcccagggg agaagggacg tcgtcctgtc ccggacccat
     2281 acggcgatgc gcattgccaa cgtcattacc atcccggcct gcttggggct ggccgtgtta
     2341 gccgtgccca tttcacgtct cttgtatgct acgccggctg cagggcctgc tatcggcgtc
     2401 ctgtccattt cggtctttct cgtcggcctc cagcagatta ccagtggcct tctgcagggc
     2461 atggggcgga cggccgtgcc gctctacaat atggccgctg cagctgtcgt taagattttc
     2521 ttgagctggc acctgacggc cattccctgg ctcggtgaag tcggggccgc ttgggcgacc
     2581 aatgccgatt tagccgtagc tacagccttg aacctctatt ttgcccgccg cttcgtcggc
     2641 tacggcgtcc agtggggcta tgtcggccgc ttgttcctgt cgggggctgc catggccggt
     2701 acggcttgga tcatccacca cagcctgctc aacctggtag ggaatacgtt ggccaccatc
     2761 atggccatga cggctgctgc cgtcgtctat ctcatcggca tctttgcctt ccgggccgtt
     2821 gccgtaacgg accttgccaa actgcccgtc atcgggtcga aacttgccgc catggctgaa
     2881 aaattaggaa aataatacag atataagagg attgccttcg taagggaatc ctcttttttt
     2941 cttgcttttg gcaaatccct ttgctatact ataagaaaat ggttgattca atcaactaat
     3001 aaaggggaca ataagatgaa ttacttatgt gtcggtcacg atttcggggt tatcaatatg
     3061 cggtcccagg cctatattac cgaagcctgt cagccttgga acctgtctta ttcagaatac
     3121 gtgaccctca tgaccctcta tgatttcgac ggctccagcc aaagcgagct ctgtaacgag
     3181 atgaaagccg ataaggccct cgttgctcgc agcctgaaaa ccttggaaga gaaaggctac
     3241 gtctgccgcc gccaaaagga cggcgaccgg cgctttaagt ttatctatct gacgcctaaa
     3301 gccagggata ttcaagggga gttggcggga tttttagaaa cctggattac ctatctcgta
     3361 gaaggttttg atgaaacgaa gcgcgatgag atgcttacca tgctgcacgc cattgccgag
     3421 cgggctgatg aagccgatat acgcgctgtg aagaaaaagg gggagatgat acgatgaaag
     3481 ggtggcatat gaagtacatc ctcgccgcag ctgccatcgt ggcggccgtg actttagccg
     3541 gctgcggcag tgagcagacc gcggccgaac cggatcaatt ggtaaagacc atggaagtcg
     3601 gaaaaacgaa tgacagtacg gccggtacct attcggggac ggtaaaaggg cgctatgaaa
     3661 gtaagctctc tttccaggcc ggcggccgga ttacgacgcg caatgttcag ctaggaacgc
     3721 gggtcaaggc cggcgacgtc ttgatgaccg tcgatcccaa ggacatcatg caggccgtca
     3781 accagtctca ggctcaggtc gatgcggcag cggctcagct ccaattggcg gcgtctaatt
     3841 taaagcgcta taaggaacta tatgcctcgg atgccgtcag cgccgctgac ctcgatcagt
     3901 tccagacagc ctatgaccgg gccgcagccc agtataatca ggctcaggcc ggccagcagg
     3961 cccaggacaa tcagctgtcc tatacacaac tgacggccga tgccgacggc gtcatcgctg
     4021 ctgtcagtgc cgaagtggga caggtcatcc cggccggtca gcctgtcgtc accttggtcc
     4081 atgacggcga cctggaagtc accattaacg taccggaaaa taaactttcc gactttcctg
     4141 tcggaaaagc cgtgaccgta tccttctggg cccttcagaa ccagaccgtg tccggcgtcg
     4201 tccgcgaagt ggcgcctatg gccgatgccg tatcgcggac ctatgaagtc aatattaccc
     4261 tgcaaaatcc gccccagggc ctgcaattgg gcatgacggc atcggttgcc aatgccggtg
     4321 aagaacgggc tgccgccgat acggtcgtcc tgccgttatc ggccatttat cagaccggca
     4381 gtacgcctca ggtctgggtc gtcggtaaag acaagacctt gtccctgaaa aatgtgtccg
     4441 ttgaaacctt tggcgacaac agcgtcaagg tcacaggcct tcaaaaaggg gatattgtcg
     4501 ttacggccgg cgtccataaa ttacgggccg gcgaaaaagt ccgcattgag ggggacgatg
     4561 tatgagaaac ttatcagaag tatccctccg taaccgggcc ttagtctggt atttcatcat
     4621 cctcgtggcc attgccggcg tcttttccta tatcaaattg ggacggatgg aagacccgac
     4681 gtataccgta cggaccatga tcgtcaccgt cgcctggccg ggagctacgg cagaacagat
     4741 ggaagaacag gtgaccgaca agattgaaaa gaaactccag gacacgccgc acctcgatta
     4801 ccttaagagt tattcccggc cggggcaatc ggtcatttac gtcaatttgg acgataaggc
     4861 gccgcaaagc agcatccgcg atacgtggca cgaagtcaga aacttgacgg aagacgtcaa
     4921 acgcgacttg cccgacggcg tctacggtcc gtactatgac gaccgcttcg acgacgtctt
     4981 cggctccatc tatgccctca cgggcgacgg ctattcttat gaagaactgc ggcaaaaagc
     5041 cgaaaagata cgccgcatga tgatgaacgt cgacgacgtc agcaaagtcg aactcgtcgg
     5101 tgaacagtca gaaaaaatct atatcgaagt ggccnnnnnn nnnnggccga actgggcatt
     5161 ccgccgacgg tcatcgccaa tgccgtcaag ggccaaaatg agatgacgcc ggccggcatg
     5221 gtgaacaccg agtcggacaa cgtttatctt cgcctgtccg gcgtctttaa ggacgtcgaa
     5281 ggcatccgca acctgcccat caatgccaac ggccgcatct tccgcttagg cgacatcgca
     5341 accgtcgaac ggcgttatac cgaaccggcc gaccctaaga tgttctataa cggcaagcct
     5401 gccgtcggca tcgccctgtc catgaaggac ggcggcaaca atttaaagct cggcgaaaac
     5461 ctgaaaaagc ttgaagccag cgtcaaagcc gaccttccgg ccggcatgga aatgaaccag
     5521 gtctcggacc agccgcagat cgtcaaagag tccatcagtg actttacgga aacactgtgg
     5581 gaagccatcg ccatcgtcct cgtcgtcagc ttcatcaccc tgggcctgcg gacgggcctg
     5641 gtcgtcgcct tctgcattcc cttggtcctt acgggcgtct tctgcgtcat ggaaatcacc
     5701 ggtatcgacc ttcataaggt atccttaggg gccctcatca tcgccttggg cctcctcgtc
     5761 gacgacgcca tcattgccgt cgaaatgatg accgtccagc tcgaaaaagg gaggagccgc
     5821 atcgacgccg cctgctacgc ctttcgggct acggccaagc ccatgctgac cgggaccctc
     5881 attacctgcg ccggcttcat tcccgtcgcc ttttccgatg gcgcagccgc tgaattttgt
     5941 aaagccctat ttccggtcat cacggcagct ttggtcctgt cgtggatcgt atcggtcatg
     6001 gtagcacctc tttttggcta ctacctcatc aagcccaaac agaaagaggg cgaggacccg
     6061 gaaaagctgt ataccagtcg gttctacgtc tttttccgca aggtcctgaa ctggtgcctg
     6121 acccatcgcg tcatcgtcat cggcgggacg gctgcagtct tcgtcatttc ggccgggctg
     6181 acctatttta ttccgaaaga attctttcct ccgtccctgc ggccggaact catcgttgaa
     6241 atgaccctgc ccgagggctc ctctatggaa gcgacccaga aagaagcgtc ccggttggct
     6301 cagtaccttg acggccagca ggataaattg aagaattact cgtatcacgt cggccaagga
     6361 gcaccgcggt tcgtcctgac tgtcgagccc gtccttccgg ccgataatta cgcccagttc
     6421 gtcgtcgtcg ccaaggatac agaatcgcgg gaagccctgg cagcctcctt gaagacggag
     6481 ctggccgata agttccccaa cgtccgcagc aatattaagc acatctcttt ggggccgccg
     6541 tcggattatc ccatcatgat gcgcgtatcg ggttacgata aggacaaggt caaagcctat
     6601 gccgaagaga ttgccgcccg cattgccaaa gacccgaata cggaaaacgt cttcctcaac
     6661 tgggatcaaa aagataaggt tatgcacgtc gagctcgacc aggataaact gcgggccatg
     6721 gggatttccg gtcaggatat tgcccagacc ctgtatacgg aactgtccgg ggcg
//
LOCUS       DAWDXZ010000027         6615 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3546_length_6615_cov_8.167226, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000027 DAWDXZ010000000
VERSION     DAWDXZ010000027.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3546_length_6615_cov_8.167226"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    850..877
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3887..3896
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5470..5529
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1405 a         1793 c         1505 g         1814 t
ORIGIN      
        1 cactcgataa tcagttcgtc atggacatgg ccgacgatgg cgcagcatcg cagcgtctgc
       61 atggcatagc agaggaggtc ccggctgatg ccctggacga tgttctccac gaacttcggg
      121 ccatagcttt cgagccgttc ccacttcttc gttgcgccga tgccttcata ggtgacggat
      181 tccccgccga agcggttctc gcctatccgg ggctttacgt aggaaagccg ccgtccgctg
      241 gggagctgga tgaacagcat gccgctctga tacaggaagc ggatgcagcc agtccgcatg
      301 gggatacgtt ccttgatggc tgtcttcacg gcggcgtcca cctgccacca gaaatcgacg
      361 atgtgcggat tggctgaccg ccaggactgc accagaggat acagctcatt ttccgtaagt
      421 cccatgtcca gggcgcccat agccttcaat gcgccaacag agccgccata gcccagggcc
      481 agttctgcga ttttcccttt ctgccggaga tacccgttga cgccatgctt ttccacggga
      541 acgccgaaca tagagctggc cgaagcacag taaatgtcac cattcctggc aaagacatcc
      601 gaacgccatg tttctcctgc cagccacgaa agcaccctgg cttcaatggc cgaaaagtcc
      661 gctacggcaa acttcagccc cttccgtggc acaaaggccg tacggatcag ctgggaaagg
      721 acattgggaa tggaatcata caggagttcc agggcttcat aatttccctg gcgtaccagt
      781 tccctggctt ccgagaggtc cggcagatga ttctggggca gattctgcag ctggatgtgt
      841 cggccggcan nnnnnnnnnn nnnnnnnnnn nnnnnnngaa ctggaacatc cctctggccc
      901 ggccatcctc gcaggccgtc atccccatgg cctggtattt tcggaccgat gacttggcca
      961 gcttctgccg gagcagcagc acactgcgaa gcggttcttc tgccgtcttc agcagtttct
     1021 gtacctgctt cttgcccaag gaatccgtct tcatcccatg ctgttccagc cagccgatca
     1081 tctggatgac ggagttcggg tctccaggcc cgtcttttcc ttcagcatag ccatcaggct
     1141 gtcccggctg cgggcatcga tgacgatggc attttcagcc agcgcccggt cgatggcgat
     1201 gccccggtca ttgatttcct ggtcgagatg atattcatcc catatcggtt ccgggacggg
     1261 atacttcttc agccgctcct ggatggccat ttccacttcc acatcccgtt tgttgtagga
     1321 cttaaacagt atccatttat ccggcgcatg ctgaggaagg tttcttgtcc tgccgccatt
     1381 cgatttggtt tccttacagg gaacgcagaa atagcggatc aggtctttgc cttccttcat
     1441 cttctggctg tccagcttca gcacggctcc tgcgccttcc agggaaaggg gcaggcccat
     1501 ataggccgac cagatcatgg agcatttcca tcctgccgga ttgaggaacc tggcacagtc
     1561 ctggaaaagc ggatgatggt cacggaacgg gtccaggctc atccccaggt cacacaggta
     1621 gcgcgacagg cagacccgtt caaagctggc attgaacgcc cacttggtaa cggattcatc
     1681 ggtcagggca tccaggatag cgttggggat acgctctccc tgcgccaggt caacgacctg
     1741 cactttgccg ccgtccaccg aatatccaaa aagaagaatt tcaaaggctg gcgattcggc
     1801 atatttgtac acgccgcatt ttgccagatt gatatcgctg aatgtttcaa tatcgatact
     1861 gatggttttc atacgcttca cctcgaaaaa acggcgaggc acaaggcccc gccgctattc
     1921 actactactt atttccggaa ggcttccatc tgcttgcggt ggtactcttc ttcccgttca
     1981 tcccggtgcc gagccatttc ttcatcccgc tggtcttttt tgatatccgt atagatcata
     2041 gccacgaaga atcctccggc gcacagtgca accaggcagt acaggccatc cagaatcagt
     2101 ctcatcatag tttccataat tgcacctcct tatgccagga aatcatcatc gtcagccgta
     2161 gcaaagtcat cttctgcacg cggcttgccg ccaaggggtt caccatcacg gattttctgc
     2221 aggttgttca ggccgcaggc gatacccttg ttgccattac tgttgaaggc atagaagttg
     2281 atggatgcac ggccatagac gccggagtag acttcagagc gttccaggat atgctggcag
     2341 tcagcatcaa cgatgcccgg cttggtcgcc gagttggcat tgacgaagaa gctgtcttta
     2401 taggcatcat cgcccgggcg ttccaggtcg ccgtcacgga gcggcgtttt gatggcttcg
     2461 agagccggta cagtgcggcc attgcccttg agcttgcttt cgccttcttc gtaagcagcc
     2521 ttgatggcag cgcggatttt ttctacggtc tttgtatccg acttagggat gatcaggctg
     2581 acgctgtatt tcggcgtacc gccattgatg gacttcggtt cccagacgtt ggcataagac
     2641 catctggtat tgactccggt aatcactttg cacggattga cataattctt ggacataaca
     2701 agttcctcct tatttttcat cattgaaatc atctgccgcg gtgtacatgg ccggacgctt
     2761 atccgattcc ggcaccagga ccggcttgcc ctgcggcttt tccactaaac ctgacagcag
     2821 ttcttcgaac cgcttcttgc cgagctgttt cgtcatcgcc gtgatgccga gcagcttctt
     2881 ttcatacggg ttgaaccctg tcttttctac agcttctgcc accgcttctt catttacgta
     2941 gcggcggttc gaccggcctt cgaccagttt ccatccgctc cactgcttgc cggaaagagc
     3001 ctgctgcaaa gcatattcct tgacatcccc ggcccagttc accagttcat cggccctctc
     3061 caggacggct tcgatttctt catcctgcag cgtggacggg acggcgaaat cataccgggc
     3121 cagttccaga ttgtactcgg cccgcttgcg gcaggttgcc ttgatcttgc agaagcggca
     3181 gtggtcaccg gctttatatt cgccctcacc tttcgccgcc agttctgccg caggcttcag
     3241 caccgtttcg gcccactgga gcagttcttc cttgctcatg gtgcaggtgc tgacgttgtc
     3301 ccggcggggc tggaagatag tcatggacac ctggcggata tcatagatgc catcaaacag
     3361 gttcagcgca ccgagggcat agcacatcat ctgcggattc ttctcggaat ccaccaggac
     3421 tcccaggcca tgcttgtaat cgatgactgt cagggtatcg tcggctacga tgaggcagtc
     3481 ccccgttcca aatcctcctg gcacccattt ggaaaagtcc agccgctgtt cgatcataat
     3541 cagcggatcc ttgcaggatg ctttagctgt agccaggcat tccatgacga actgcgcgta
     3601 ttcatcagtg cattccgcca tctcctcatc aaagaacgtg agtcccttcg tcgggtcttc
     3661 cagtttctgg cccaaggccg tcttcacctt gaattcacag agcgtatggg catccgtccc
     3721 ctgccgggcg aattcactgg aggtatccgg cagcttggca cattcctttg cagatggtgg
     3781 gcaggccagc cagcggtagc aggaagatgc ggacagcacc gcatgtttat ccggcatggc
     3841 caatcacctc cagttccttc aggaacgctt cgtactgtgc tgcatcnnnn nnnnnnctgc
     3901 atcaataccg gacagcttgt ccgccccata tttctggatg aggctgcgga cttctgcggt
     3961 gaatcccttg cgggccttgt cggcagcgac tttgcggaca tcttccagtg tcagcggctt
     4021 ttccaatttc tctgatttcg cttccgaagc tgtcggatta tcttctttca ccgccatgac
     4081 ttcggaaatc ttcagcagcg ccttaccgca atcgcccagg gctgctgcca gtttctgcag
     4141 ttcatcgttt ttcatatgga ttgactcctt tcacatgtct ttgcattgat aagagatgga
     4201 tatttctggc aatactgcgg gtcgtgacgc tgatggccat cagcaccgcc gccagttctc
     4261 ggtccagctt ttgctgctga gccagttttt caggtgtctg tgtgtacatc atcttgagtt
     4321 cctccttcct gaatggctta tccgttcgcc cttcaccagt aataggacaa ccgtgtcatc
     4381 gttaagtacc gatttcaaaa aaaatccggc cacatttttt gtgaccgggt cttttttctt
     4441 cttaacggaa gtctttgaga tgttccttca ggagtgcata cagcttgtgt ttgcgcttgt
     4501 tcaccgcttt ctggctcaag ccgacggctt tcccggtcgc cgcttcgctg gcaccatcgg
     4561 caagcatcag caggatggtc cggtcgatat cctgcaaggc cgccagttct tggcgcagcg
     4621 ctgccagtaa ctcttccttc acgacttctt cctctaggtt gaagtcatca ggcaccgcca
     4681 gttcatagtc atcctgcgcc ctgtcagctg atacctcatc ggtaccatgc ctttgctggc
     4741 gcttaccttc ccgccaaagc ggacgcatga attcatagta ctgctctttg gttgctggaa
     4801 tcaggatagt acggacctta cggcaaccga tttttgacca gcggatttca cagtccttgt
     4861 atttctcggt gatgactgtt tctggcgtga gttccagagg aatgtaaaaa cgtttctcat
     4921 tgcttgtctg tcttttgtcc atttttgtct ccattccgtc cgagaagaac ggcggagaca
     4981 aaaagctggc actgatacag catcagcgcc agttttctga tagcccaaat aggcatagaa
     5041 aaacacggtg gatgcatgat cagcaaagta taaccatcgg ctgtactttc tgctttatgc
     5101 aaccgccgcc cttatagcgc tattcgaact acgatttatt tgtggccagg ggctctctcc
     5161 cttgcctacc tatagtctat gccaaaatca aaatcgtgta attgacatga agtggcgtaa
     5221 aatggaataa aatggtttgc tgaaaaaagg ccaaaaaaaa ataggcttct caccttagtg
     5281 agaaacctat tgcaaatagt ttatccttga tatccgtcaa tcaagccata tctccctatc
     5341 cctttgtttt ttgtaaagtg aataagattt gcaccttcat aatcgataga tggaatttca
     5401 ttttcaataa caatcgtttg tggagaatcc gcccttgtca aaaaatatct aaacaaatgc
     5461 tctttcatgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnc agcaaatctc aatgaaattt taaaatcatc aagttgcttt ttcaactgcc
     5581 ggattttagg ttcaagttct cctcgtattt tttcttcctg ctcatttctc tgattaataa
     5641 gcatatctct tttctttgac aaagcagcat attcttcccg tatagattcc tggactgatt
     5701 gcaaatccct gacctgcatt tcaatttttt gttcttctgc tatcgcagaa cctatacaat
     5761 cctcttcatg ttcatgagga agttctccat tgcagaaagg gcacctttct attgttttga
     5821 ttttttgcga aaccatattc ccttccgcaa taaaggtcag gcgcttaata tccgccttat
     5881 attgtgataa aaggagttga ttccgattca tcaaaacctc atcttccgaa atctgccgca
     5941 ttactttatt gattttctct ccaatttcat gacatgaggt caaagcttct ttaaggattc
     6001 ctttagcagc tccgatttca tcgattgttt gattgatttt attctgcaat tcaacgggtt
     6061 gaagcaaagg taatgtatca tcaaaagatg actttttcct ttctaagaat cccatgcctc
     6121 tgttgacaat tttcagcact ccctcatctt ttgctttctt tattttggga tcaataaaag
     6181 ccgcctttgg aagatagttg ttacccgtcc ccaagtacaa taatgcggtt aaagtcgggg
     6241 ttcctacctt tcctcctaat ccgtgctttc ctttaagaac actggcttta tcatgaattc
     6301 tgtcctcatc tatgatgaac gtatgataaa acgttctaac cgtcaaagcc tgcggttttc
     6361 cttcctgcgt tttaagaatc ttagtatcaa gaggaatgtc caaaagcttc atccagacgt
     6421 cattaatagg tggattcttt ttccctccct ttatcttgta agtagctgtt tctataccgt
     6481 ctacatgact aattacttcg aaagatttgg tattaatatc ccggctgata gaaaggcttt
     6541 ccccattgac atctattcct atttgaatct tctccacttt caaattaata tcgaatcgat
     6601 gagtaccggc tccca
//
LOCUS       DAWDXZ010000028         6604 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3553_length_6604_cov_8.557032, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000028 DAWDXZ010000000
VERSION     DAWDXZ010000028.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6604)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6604)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6604
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3553_length_6604_cov_8.557032"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    288..297
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    831..840
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2997..3006
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5249..5258
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5827..5919
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1635 a         1798 c         1706 g         1332 t
ORIGIN      
        1 catcaacgtc ggctgcattc cgtccaaacg cctcattacc gaagcggcaa cggctccgac
       61 gtctgatttc gccgcgaaag ctgcgtatta taaaaaagcc gtcgaaggca aacgcgaatt
      121 gacgggcgcc ttgaacaagg ctaactacaa taaactgatc caggccggcg tcgaagtcat
      181 cgatggcatg gcatccttcg tcgatgcaac gcacgtcgaa gtcaagacga aagacggcgt
      241 cctccagctc gaaggcaagc agttcgtcat caacaccggc gctacgtnnn nnnnnnngga
      301 tatcgacgtc ttcctgcctc gcgaagacaa ggatatcgcc gataatatcc gttccgtcct
      361 cgaagccaag ggcattgaaa tcgtaaccgg tgccaagacg acgaaaatcg aaggggcaac
      421 cctctattac accgtcggcg atacggatac ggtcttagaa ggcgacgcca tcttgatggc
      481 taccggccgc cgtcctaaca cggatggcct ggaagctcag aaagccggca ttgccctctc
      541 cgacagaggc gctgtcgtaa cggacgaaca cctgcggacc aatgttccca acatctgggc
      601 tgccggcgac gtctgcggca atctccagtt cacctacatt tccctcgacg acagccgtat
      661 cattatgtct gacatgagcg gtaaaggtga ccggacgaca cagaaccgtg gtgccttctc
      721 gtactcggtc ttcatcgatc cgcccttctc gcgggtcggc ctcagcgaaa gcgaagccaa
      781 ggccaagaac atcgaatatc gggtcatgaa tctgccggct gcggccattc nnnnnnnnnn
      841 cgtcctcaag gctttagtcg gtgccgacga ccagatcctc ggcgttcagc tcttctgcgc
      901 tgaatcccat gaaatgatca accttgttaa actcgctatg gacaacaact tgaaatacca
      961 ggtcctgcgc gacttcatct atacccatcc gacgatgtcg gaaagcttga acgatttgtt
     1021 cgctttctaa atttgtaaca taaccacact cagacagcgg ccgtcttcga atcattttcg
     1081 gagacgccga gccgctttgc atataaggat acagcctgcg gcttgctgtc ggcgttggaa
     1141 taaaaagctt ctcgggttcg cagcagcgga gttcgggaag ctttttttga aaggatgata
     1201 acatgagtat tagtaaggaa accctcgttt cccgtaaaga aattatgaaa ggacgggtgc
     1261 tccacgtaac ggtcgacacc gtgtccattg atgacggtca gggtggggaa aagaagacgg
     1321 ccacccgtga agccgtttgg catttcggcg cctgcgccat tttgcccttg accgatgacg
     1381 gcaagattat ccttgtccgc cagtaccgct atgcagcagc agaagctatg ctggaagtgc
     1441 cggccgggaa aatcgaacac gacggcgaat cgccggatta ctgcgccgcc cgggaactcg
     1501 aagaagaagc gggcgtgacg gctaaagaaa tcctgcccat gggctacgtc tatacgtcgc
     1561 ccggtttttg caacgaacgg atttacctat atttagccag aggccttgaa aaggggaaac
     1621 agcacctcga cgacgacgaa ttcatgaacg tcgaatacta tacgccccaa gaagtggaag
     1681 ccctcatcga taagaacgaa attaccgatg ccaagaccat tgccgctttt gccaaggctc
     1741 gtaaatatct gccgggatta taggaagggg gagcacatga aattcaatcg catcatcgtc
     1801 atcgttaccg acagcgtcgg cgccggcgga gcccccgatg ccgaacgctt cggcgataaa
     1861 ggggccgata cctacggcca catcgacgcc aaggtcccct tgaacctgcc taatctgcgc
     1921 cgcatgggcc tcgggaaggt tgccaacatt catcatcatg aagatacgga cgtcgtcggc
     1981 tcctacggcc tcatggaaga aatctccatg ggcaaggata cgaccagcgg ccactgggaa
     2041 tttatgggaa accccgtcga aaatcccttc ccgacctttg aaaaggcctt tccgaaagat
     2101 ctccttgata cctttacgga aaagacgggc tatggctaca tcggcaatga aatcgcttcg
     2161 ggaacggaaa tcatcaatcg cctcggcgcc gaacacttca agaccggtaa gcccatcgtc
     2221 tatacgtcgg ccgacagcgt tttccaaatc gctgctcaca acgacgtcat tcctttagaa
     2281 gaattgtacc gcatctgcga cattacgcgc cgcgaagtct gcgtcggccc atatgaagtg
     2341 ggccgcatca tcgcccgtcc cttcatcggc gaacagggcc attttgtccg taccggcgac
     2401 cgccgcgact acagcagcct gccgaagcgg aagatggtct tctcctactt atcggaagcg
     2461 ggatacgccg tcgtcggtgt cgggaaaatc ggcgatatct atgcccatat cggcctgacc
     2521 gagtcgtatc acacggccaa taaccacgaa gacatggtgg tcctgcggga acaattagcc
     2581 gcccaccgca agaatcaggg cctgctcatg gccaacttcg tcgacttcga cagcatgtac
     2641 ggccatcgcc gcgacgtcga aggctacgcc aattgcctgg aaagcttcga taaggactta
     2701 ggacccctca tggcggaaat tcaggatgac gaacttctgg tcatcacggc cgatcacggc
     2761 aacgatccga cctggcacgg cacggatcac acgcgggaac gcgtcccggt cctggcctac
     2821 agcccgtccc ttaaagacgc tgtcgacctc ggcattcgca agacctatgc cgatttgggg
     2881 atgaccatca tggataattt cggcctcaca ggccttcaat tcggaaccag ctttttaaca
     2941 gaactgaggt gatcctatgt ggccgatgga ctgcagcgcc catgaacgag tcggcgnnnn
     3001 nnnnnnggaa gaaaagtttt tagaccaagt gcgccgcgtc ggcatttccc tcattaccca
     3061 gtccggtgaa atcgcagcgg ctgataaatt gctatatgcc ctgcgcgatg cgacggcaac
     3121 cgtcgaaagc atccccctca ttgccagctc catcatgagc aagaaaatcg cctccggcgc
     3181 cgatgccatt gtcctcgacg tcaaatacgg cgacggggct ttcatgaaga ccaaagaacg
     3241 ggcccaggaa ctggccaaaa tcatggtcag catcggccgc ctcgccggtc gtccgacgcg
     3301 ggctgtcatt acgtctatgt cggcgccctt ggggacggcc atcggcaatg ccctcgaagt
     3361 cgacgaagcc gtcgaagcct tgtccggtaa aggcgggcgg cgtctcatgg aagtcgtcga
     3421 agccctgggt gctcagatgc tcatcgtcgg ccaaaaagca gccgatgaag cccagggacg
     3481 ggctatgatc cgcgaactga tcgcttccgg aaaaggcctg tctaagttta aagaatgcat
     3541 caaggcccaa gggggcagta cgtcgtggat cggcaagcgc ccccttacca aggcggaaca
     3601 ggtctttacg gcagtcagta ccgacggcgg atatatcaca cgcatcgatg ggagggctct
     3661 cggcgaaatc gccatggaaa tgggcgctgg tcgggcccgg aaggaagacg ccatcgagcc
     3721 catggtcggt atccgtctct ttaaggaact ctacgatccc gtacggcccg gcgaagcctt
     3781 attcaccttg tacggaaaga aaggcgacga tatgatggcc ctcgctcagc gcgccgccga
     3841 ccatatcatc gtgaccgacg aacaggccgc tgttcaggaa cctgtcgtca gcgacatcat
     3901 cttataaagg gcagagaaaa ggccttgtat cgttttttag aagcgtacaa ggcccctttg
     3961 ccaagaaagg aacctcatga aagaaacacg gcaaatccct gtgcagaagg ggaaaaccta
     4021 tgaattgtcc attgccggcc tgggaacgag cggcgaaggc attggccgct atgaaggctt
     4081 tacggtcttc gtgccctatg ccctgcccgg tgaaacggtg cgggcccggg tgacgatggt
     4141 taagaaaacc tacgcctcgg cggccttaga acaaatcctg actccgtcgc cggatcggga
     4201 aaagccgccc tgtccggtct atgaacgctg cggcggctgt cagctccagc acctcagcta
     4261 tgaaggcgag ctgaaggaaa aacggcagca ggtaaaagac gccatggaac gcatcggtca
     4321 cttgcagggc gtcgacatcc tgcccgtcct cggcgccaag acaccgtgga actaccgcaa
     4381 taagatgcag tttcccgtag ccgcggcggg taagcgaaac gtcgccatcg gctgctttgc
     4441 agcctcgaca catgaagtca tcgacgttga aaactgtgcc attcaaaaag aagccaataa
     4501 cgtcatcgcc gccgtcgtcc gtcaatggat gaaagatttc aagattccgg cctacgacga
     4561 agacgcacgg acgggcatcg tccgtcacat catgggccgc gtcggcgtcc atacgggaga
     4621 agtcatggtc tgcctggtaa cggcttccga catggtcccc catatgaaag atttggtcca
     4681 atacctgcgc cgcgacgtgc cgggccttaa gagcgttgtc caaaacgtca acaaacgcca
     4741 taccaacgtc atcttaggaa ataagaccag gaccatttac ggcagcggca ccattcacga
     4801 ctccatcggt tcactgacct tccatatctc ggcccagtcc ttcttccagg tcaatagcga
     4861 acaggcccag cgcctttatg aaacggccct cgactttgcc gatttgaaag gcggggaaat
     4921 cgttgccgac gtctactgcg gcacggggac gatcaccctg ttcttagctc agaaggccaa
     4981 acaagtctac ggcatcgaaa tcgtagactc cgccatccgt gacgctgtcc gcaatgccaa
     5041 ggacaataaa gtcggcaacg cccaattcat cttgggcgac gccatctata aactgcccga
     5101 actcatccgt aacggcgtcc ggcctgacgt catcgtctta gacccgcccc gtgccggctg
     5161 cggcgaaccg gtcttaaaag ccatcgccaa cagcaaacca aagcgcgtcg tctacgtctc
     5221 gtgtaacccg gcgacattgg ctcgcgacnn nnnnnnnnaa caatcacggc taccatacac
     5281 aaaaagtaca gcccgtcgac atgttcccac ggactcacca tgttgaagcc gtggcgctca
     5341 ttacaaggga aaagtaagga tcgagtgatt gagcctcatt cggaatttga aaaggggaga
     5401 gcatcagaaa aaatgggctt tcatacggtt gaatccgttg aaaacaccga gtggagtaca
     5461 aagggaactc ctgtcatgga agtcaaaatg gaaatggatt tgtgagtaca taagcggata
     5521 catccccgtt taatgtcatg aagcgaggct ctcagtcact atcgtgaaat agatgattgg
     5581 gagccttttt atatgcaaaa ttttataaat tctgcaaggg gttaatgtaa catggttgtg
     5641 gggaaatttt aacgtaatcc gctggaaatt atccacctct gaggtagtgg atgaaggcga
     5701 actggcgact ttgcgtaagt aattaagcca aataattgct ctgtggatag ttttttagta
     5761 taatcagtaa tatgataacg gattgattat aatactgaat tgcaaaataa taaccattca
     5821 gcagtannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tgcagccttg ccattaccgc
     5941 ggcagcaggt tttaatgtgg ctgaggcagc tcatggagac aaggtaaata ttgccgagcc
     6001 cttcaaggct actgtccttg tcgatggcct tggttctccc tgggaagtcc tttggggacc
     6061 ggacgattcg ttgtgggtaa cggaacggca gtcgaaatcg atttctaagg ttgacccgaa
     6121 aacgggtaag catacggtgc tgtatacctt caaaaatgcc gtggcagcgc cgcctcatca
     6181 aggggttctg gggctggcat tagatcctca gttcctgaag ggtaataact atgtctacac
     6241 ggcctatacc tatgaagtca atggtgaaaa gcatgcccgt attgtccgca tgacctatga
     6301 tcctaagacc gagaccctcc gtgatgaaac ggctatcctc gataacctgc cggccagcgt
     6361 tgaccataac tccggtcgtc tccgttttgg accggacggc aagctgtact ataccatcgg
     6421 tgaccaaggg tataatcagg gatctcacgt agacaaggaa atccaggcgc agatgctgcc
     6481 gacggcagag caggttgctg ctaaagatta cagcctctat ccgggcaaag tgttgcgtct
     6541 caataaagac ggctccattc ccgatgacaa ccccgtatta aacggtgtcc gcagtcatgt
     6601 atat
//
LOCUS       DAWDXZ010000029         6597 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3558_length_6597_cov_9.446347, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000029 DAWDXZ010000000
VERSION     DAWDXZ010000029.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3558_length_6597_cov_9.446347"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1521 a         1574 c         1904 g         1598 t
ORIGIN      
        1 tacctccagt cgatgaaggc cttccttgaa aaggaactgg gcgttgaaaa agttcgcctg
       61 ctctgcccct tgtcggatgc cccgcagtgg ctcttgaagg acatggaagt ggccagcgtc
      121 gaagccgtaa tccgccagga atgccgcaag gcgcggcggg tcattgccga tccgatttat
      181 gctcggttac tgccggatga gcctgataaa tttatttcca tgcctcacga agcctattcc
      241 ggccgtcatt atcacgacga tatgcctatt tttgtcggct cgtcgtttac ggcgtggatg
      301 aaagataaac tgaaataaga aggagactga ctatgcggaa atttgccatt tatggaaagg
      361 gcggcattgg gaaatcgacg acgacggcga acgtgtcggc ggccttggcg tcgattggaa
      421 aaaaagtcgt ccaaatcggc tgcgacccga aagccgactc gaccttgaat ctgctccatg
      481 gcgaaccggt catgccggtc atggattact atcgccaata tacggaaggt ccggacttcg
      541 atcatatcgg acgggaaggc tacggcggcg tcttctgcat cgaaacgggc ggaccgacgc
      601 cgggccttgg ctgtgccgga aggggtatca ttgctacctt tgaccttatc gacgaattgg
      661 aagtctttga aaaactttcg ccggacgtcg tcatgtatga cgtcttgggc gacgtcgtct
      721 gcggcggctt ttcggcaccg atccgcgacg gctatgccga tgaaatcttc atcgtcacgt
      781 cgggggaaaa gatggccctc tatgcggcgg ccaatatctt taaagccgta gaaaacttta
      841 agagccgcgg ttatgcccaa gtaaaagggc tgatcctcaa tcgccgcaac gtcgatgatg
      901 aatatgaaac ggtcaaggct tttgccgatg aacacggtct gcctatcgtg gccgacattc
      961 cccgcagcaa ggaaatccag ttttatgaaa acaagggaat gaccgtcgtc gaaggcgatc
     1021 cggagctgcc cgtaagccga atctaccgcg atttggctca gcggctgctg gaagtataga
     1081 aaggaagtaa ggccatggga atgaatgaca cgccgtcagg cgaacgcgtc catatcggat
     1141 ttttcggccg ccgcaatgcc gggaaatcaa gtgtcgtaaa cgccgttacc ggccagaacc
     1201 tgtctatcgt atcggatacc aaggggacga caacggaccc cgtttacaag gccatggagc
     1261 tcctgccctt ggggccggtg gtcatcatcg acacgccggg catcgacgac gtcggtgccc
     1321 tcggtgaaga acgggtgcgc cggacgaaac aggtcctcaa taagaccgat attgccgtcg
     1381 tcatcgtcga tgggacggaa ggcttggctc cggctgatga agaggtattg gccctcattc
     1441 ggagcaaggg cctgccttat cttatcgtct ataataaggc cgacctggtg aagcatgtgg
     1501 ctgaggccga tgatcatgca attcatgtca gcgccttgac cggcgacggc atttacgaat
     1561 tgaaggaaaa actggcccgg ttgaagccgg ctgacgccga ggctcccatc gtcagcgacc
     1621 tcattcatag cggcgacatg gttgttctcg tcgtgcccat cgattcggca gcgcctaagg
     1681 gacgcctcat cctgccgcag cagcagacga ttcgggaaat cctcgacagt aacagtgcgg
     1741 ccatcgtcgt caaggaagat gaactgcccc ataccttgtc ctgctttacg aagaagccgt
     1801 cgttggtcat tacggacagt caggtctttg gacgggtctc ggctgatacg ccggacgata
     1861 tccgcctgac gtccttttcg attttaatgg ctcgccataa gggcctcttg gaatcggctg
     1921 tccgcggtgt caccgccgtc gataagctga aagacggaga tacggtcctc attgccgaag
     1981 gctgtacaca ccatcgccaa tgcgacgaca tcggtacggt aaagattcct cgctggttga
     2041 aggagtatac gggtaaagat atctcgattg aaacggctag cggccgcgat tttcctgaag
     2101 atttatcgcc ctatgccatg gtcattcact gcggcggctg catgctcaac gggcgggaag
     2161 tcgggtaccg catgaaaacg gctgtcgatc aggccgttcc cattaccaac tatggcattg
     2221 ccattgccta tatgaaaggc atcctgcggc ggagtctgag tgtcttcccc gatttacagg
     2281 aactgctgtc ggcacattag gcgagcaaga cgaaaaaagc agcccgtgaa aggcgggttg
     2341 tgaggtggat ttatggcaaa cttatcgctt aaacttgaac aaaaaactaa attgatgcaa
     2401 gtccagcgcc tgacgattca gcttttggct ctccacggtc aggagttgat ggactttctc
     2461 caggaacagg taacggataa tcctctcctg gacatccgct atcacgacgt ccgcggagcc
     2521 ggggataaaa ctgaaaagcc catcgacaat attaaaagcc gcagtgcatc cctcgaatcg
     2581 cagctcatgg ctcagctccg cgtcgtgtcc gtgccgcggc cgattctctt ggcggcggga
     2641 ctggtcatcg gttctctcga tgagaaaggc tttttccaag gtgatttatc ctccttaggt
     2701 gacggctacg gcctttcgga agaggacatg gaaaaaggcc tggcactggt ccagaccttt
     2761 gatccgccgg gcatcggagc ccggaatatt tgtgaagccc tgcttatcca gacgcggcgc
     2821 cgcagtgacg ggccgcccaa gacagaagaa ctcttgaccc tgcactatga tgatttcctg
     2881 cgggggaagt ggtcgaaaat ccagcagggg ttggatttat cggataagga tttgcgcgac
     2941 attcgggatt ttttgaagac cttgtccctt cagccggccg gtcagatcga gcaggatgaa
     3001 gtttacatcc gccccgacgt cgagatttat cttgaagagg gcgggacatt agctttccgc
     3061 tggctggaag agcttcccga cgtctatttt cgcgatgatt tatacgccga gtatggagcc
     3121 cagaaagata agaagacact ggcttatatc cgcaaagccc ggagggcttt taacgatctg
     3181 gcgtcggctt tggcctatcg ccgcctgtcc attgaacagg tcatgaactg tctcttgact
     3241 cgtcaggaag gctattttct ccacggtcag cccttggagc cgctgcgcca aaaggacatt
     3301 gccgaggaaa cgggcctcag tacggcgacg gtaagccgcg tctgccgcaa ccggtacgcc
     3361 ctgtttggcg gtaaggtata cgggctgcag tcttttttag ctacggctta cagccgtcag
     3421 cgcgaggaag aggactttgt ttctgataag gccattatga agaaaattca ggcgcttatc
     3481 gcaaacgagg acaaggagca tccctattcg gatcaggata tagccgacta tttttcggcc
     3541 atccatattg ccatagcccg gcggacgatt acgaagttcc ggcaaaaatt aaatattccc
     3601 aacagcaata tccggcgccg atgtcgatga ggccccttac ggccgcatct tattgaagat
     3661 ggtctgccgc gatacgttga gcaatttggc tatatccttt tggctatagc ctttttcttg
     3721 aagctcttcg atgaggattt gctggatggt cacctctaaa tgcttcatgt tgagggtcaa
     3781 agacgccgtg tcgaggaggg cgtctttttt attttggtcg gcaatgtcga agagggcatc
     3841 ataggacttg tcgagggtgt cgccgaagat gaagtagcgc agggcgacgc tgtggagctc
     3901 gcggatgttg ccccaccagt tgtgctggac gaggactttt tttaaggccg ggctgagttg
     3961 gcatttttga tggcttaagg tttcgacgaa atgggtgaat aagtagagga tgtcgtcagg
     4021 ccgatcgttt aaaggcggca gattgaggcg cagggtgttg atgcggaagt ataagtcgcg
     4081 gcggaattcg ccggcttcga ccgaggcgat gaggtcggcg ttgccggctg agatgatgcg
     4141 gatgtcgagg gggatgacgt agtctgagcc gaggcgcatg acctgtttcg tctccaagac
     4201 gcgcagcagt ttcgattgga tgctgggggc catactgttg atttcgtcga ggaaaatcgt
     4261 tcccttgtgg gccatttcaa agaggccggc cttgccttct ttgcgggcgc ccgtgaagga
     4321 gccgctgacg tagccgaaga gttcgctttc gaggaggtct gtcgtcaagg cggcgcagtt
     4381 gatggccacg aaaggcccgt tgcggcggtc gctggcgttg tggatgctct gagcgaagag
     4441 ttctttccct gtaccgcttt cgccttgaat caggatgggg gcgttgtact ctgccatggt
     4501 ggcggcccgc tgaatgactt ttttcatgtt gccgtcgacg gtcttgatgt cgtcgaagtg
     4561 atgtctggcg gtcatgccgg tcttggtcag cttatagcgg atattttttt ccaggcgctg
     4621 cagctctgtg acgtcgcgga gggtcatgat atagtgggag ctgctgtcca tgaggaagaa
     4681 gtcggagttg gcgatgaggg tatagggcgg acggcggatg atcgtttcgt ggaaggacgg
     4741 tttgttccct tcgacccagt ccgggaagat atcgcggata tttttcgtcg tcgccgacac
     4801 gcccagaaag tcggcagccc ttttgttgag atgggaaatg gtgccgcctg tggaatagag
     4861 gatgatgccg tccgtgacgt gggataagat ggacgacatc atggacagct gttgattgtc
     4921 cttttctttg aatgacagca gttcttgggc gtagagcagg accgacagca tcgtcgattc
     4981 gctgggcttg atggggtaaa tcggcatcgg actgggattg gcgatttggg gcaggagggg
     5041 acagccgacg atggccgtgt cggccgtgac cggtatctgc cgcacccgtt cgaggagttc
     5101 caagtgcgtc ttatcgaggc tgtagcgtgg gtaaaatacg attttctccg acagctccgg
     5161 cggacaggct tccttgttga acatgaagtt ttcattgagc agcaagtgga tttttttgta
     5221 attcggggca atggcccgca gggcatagag ggcgtcgttg gtcgagctgt acagacagag
     5281 gatgggaatg gtgtcgatgg ccttggtcag tatctggtgg gcgccgctgg tggtgatgat
     5341 gaccttggtt cctttttcga ctaaggcatg ggcttgggcg acgaggttgg gaaagtcgat
     5401 gatttcgacg tcgatgatga agtggtgttg ttctaatttc tccttatatg tgtctaagat
     5461 ggtgtgaacc atctgctgca tggaaaggtc ggaaatcagg aaggcaattc tatccataag
     5521 tttctccttt atatttcatt aaaaattacc gaaaagttct ccttctatca taaaaaaata
     5581 tgaagagaat gtaaagtatg taacgaattt tttacatatt ttgaatatca attagcttat
     5641 caatatgaaa aaatggctta aattcgataa tatcatagtt ggcatgatta tttcatatat
     5701 attgtcgaac agataaatga gctgattcaa cgaatgagga ggtatgaatt atgaaaaaat
     5761 tattgtccgg tgacgaagcc atcgccagag gcttatatga agccggcgtt tcctttgctt
     5821 cggcttatcc cggtacgccg agtacggaaa tcttggaaaa cgtctcgact tatgatggta
     5881 tgtactctga atgggcaccg aatgaaaaag ttgctatgga agcggctatc ggcgcttcta
     5941 tgtctggtgt ccgctctttc gcagccatga agatggtcgg tgtcaacgta gcagctgacc
     6001 cgctgtttac gttctcctac ttaggcatga acggcggttt tgccttcgta tcggctgacg
     6061 atccgggaat gcattcgtcc cagaatgaac aggacaaccg caactatgct aaattcgcta
     6121 aaatcgccat gtttgaaccg tcggactccc aggaatgcat cgacatgacc aaagaagcgt
     6181 accgcgtcag cgaaaagtac ggcgttccca tgatgattcg catgacgact cgtgtctgtc
     6241 attcgaagag cctcgttgaa gaaagtgatc gtgtagaggt tccggccgtt ccctataaga
     6301 aacagcgcga aaagatggat gccgtcccgg ccatttccaa acagcgccgc tacgtcgtcg
     6361 aagaacggga acaaaaattg aaagaatacg ctgaagaatg tccttttaac gtcgtcgaaa
     6421 tgcacgatac gaagatcggc atcatctctg ccggctcggc ctatatgtat gccaaagacg
     6481 ctttaggcga caaggcttct tacttgaaac tcggctttac ctaccccttg ccgatgaaga
     6541 aaatcaaagc ttttgccgat tccgtcgatg aactgtacgt cgtcgaagaa ctcgatc
//
LOCUS       DAWDXZ010000030         6593 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3563_length_6593_cov_10.389263, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000030 DAWDXZ010000000
VERSION     DAWDXZ010000030.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6593)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3563_length_6593_cov_10.389263"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1524 a         1619 c         1770 g         1680 t
ORIGIN      
        1 tagagaaggc tccctgccag gcagggagcc tttttcgtta tcgtcgttta ctatcccaag
       61 agatcctgtt cctctgtatg gcgcttaaac caagcttggg cataggtgca gctggtcgcc
      121 gtctttttat tgcgcttacg gatttcatcg gcagcggctt tgaccaattt tccggcagcc
      181 ccttggccgc gcaggctgtc atcgacgaaa gtatggacga tgtcatagtt gccgtcgtca
      241 cgctggacga aatcgacttc accgacgacc tttccctgtt cattttcgat ccaaatctta
      301 tggtcactgt attgtaattc catgttaaaa cactcctttc gcaagggatg atgaaaaacc
      361 aacctaccag ttcatggtaa cctcgatgat ctgcccgttg cggaccgaac ggctgcgccg
      421 gaaggctgcc atatactcct cgacgggaag gttcggattt tcatagacta agagaaaatt
      481 ccgtatgaac tgggcatggg taaagactac aataaattct tccgggcgcc gcttcaaaag
      541 ggccagtgtc cgctgaatgc gctcgatgag atttcgatag ctttcggccc cttctccatc
      601 gacgtaatcg ggatcgagat tacgccaata gtcgatgact ctgggccgcc gctgggccga
      661 tgtcgtcccg acacaagtgg ccggcgccaa atagacaaat tcatggacga catcccaaat
      721 ccccgtggcc gcatcgggaa atcggccgat agtatacatg gccgtctgct gtgcccgaag
      781 atacggtgag gtcacgatga gatccggctg tttcggtatt tgcagggcca gggcctgagc
      841 ctgtttcagt cccaattcgg acagaggaat acttttgtga tcgtccgtcg cttcaccggc
      901 attggcaatg ctctcgccat ggcggatgag ccatatcctc ttcatagtaa ccacctgctt
      961 tctgtttcat tgtatcacaa actgtgcgtt ctttggaagg aaaaaggagc cccatacaaa
     1021 acgaaggctg ccggagcagc cttcgccttg gggtatgtat atggttttga gcgtgaatct
     1081 atagactgag caaatctata gtcgtgcaaa cattatttat cggccaattc tttgtatact
     1141 tcgtatttct tagcagcaaa ttcttcggcc ttggcaaaga gttcttcggc aatttccggg
     1201 aaggtacgcg taagggctgc gtatctcgtt tcgccgagga ggtaatcgcg gaaggactgg
     1261 gtcggttcct tggaatcgag gatgaacgga ttctttcctt cttccttaag gagcgggttg
     1321 tagcggtaga ggtgccagta accggcttct acggcgcgtt tcatttcatt ctgagcgttg
     1381 cccatgccgc ctttgatacc gtggttgatg cacggtgcgt aagcgatgac gatggacgga
     1441 ccgttgtaag cttcagcttc tttaacggct ttgatgagct ggttggcatc agcgccgaga
     1501 gcgacctgag cgatatagat atggccatag gtcatgagca gacggcccaa atctttcttg
     1561 ttgaacttac ggcccgaagc tgcgaactga gcgacagcac cgatcggtgt agccttggac
     1621 gactgaccgc ccgtattgga gtaaacttcc gtatcgacga gcatgatgtt gacgttggca
     1681 ttggtagcca tgacgtggtc caaaccgccg taaccgatgt cataagccca gccgtcgccg
     1741 ccgtacatcc aaatcgtctt cttgatgacg tgttctttat gagccaagat gtctttctga
     1801 aggtcagctg ctttgccggt cagcttcgct gccatcaatt ctttgaggta cgtccggctg
     1861 atgccttccg taacttcacc ttcgttggcg acaccgatag aatcgagcca agcttggata
     1921 gcatcgtgga gagcgccttc cgtcagcggc aggagttctt ccgatttcag gatgattgct
     1981 tcgcgctgct gatccatgga aagggacata ccgagggcaa attcagcatt atcttcaaag
     2041 accgagttcg accaagccgg accgaaacct tcgtggttcg tcgtatacgg aatgctcggc
     2101 atagcagagc cccaagcctg ggtacaaccc gttgcattgg caacgatcat gcgcgatccg
     2161 aagagctgcg ttaaaagctt catgtaagac gtttcggtac agccggcgca ggcaccattg
     2221 aactcgagga gcggctggtt gaactggctg cctttaacgg agaatttatc gagacccgtt
     2281 tcttttaccg acaaggtaag ggcatagtca aagttttcac tttcatgacg ctgggtatcg
     2341 aggggtttca tggtcagagc cttagccgga caagcgtttg cacaggcgct gcagctatag
     2401 cagtcgagag gcgaaacctg aatgcggaat tcataatctt cgaggccttt gccgatggct
     2461 tttttggtct tgaagctttc cgggctgttg gctttttctt cagccgtcaa gaggtacgga
     2521 cggatgacag catgcgggca gaccagggcg cagcggttac actgaacgca cttgtcgaca
     2581 tcccattcag gaaccttgga agcgacaccg cgtttttcgt actgcgtcgt gcccagcgga
     2641 taggaaccgt cagcgtaagg aacgaattcg ctgaccggaa tcgaatcgcc aatctgaagg
     2701 ttcgacggtt cgacgacgtt cttaatgaag tcaggaatat tgcgcgtatc ttcgacgacc
     2761 ggatcggacg ggaggtcttt ccattctgcc gggacttcga cttcgacgag gtcagagatg
     2821 ccacggtcga cagctgcctg gttcatggag atgacctgtt cgcctttcgc aaggtaggtc
     2881 ttagcgatag cgtctttcat atgtttaacg gcatcatcga tgggcatgat gttagccagt
     2941 ttgaagaagg ctgcctggag gaccgtattg gtacggttgc tgccgaggcc gatttcttcg
     3001 gcaatcttag tcgcattgat gatgtagaat tttacgtggt ttgcagccag ttggcgttta
     3061 accttgttgc tcaggtggtt ggcgacgtcc gctttatccc agttgcagtt gagcaggaag
     3121 atgccgcctt ctttgatttc gctgacgatg tcataggtgt ccatgtaagc ctgtttatga
     3181 catgccagga agtccgccgc ttttacgtag tacgtagccc ggatcgggtt ggtgccaaag
     3241 cgcaggtgcg acttggtaac gccgccggat ttcttggtat cgtattcgaa gtaagcctgg
     3301 acgtacatat cggtattatc gccgatgatc ttgatggagt tcttgttggc accgacagta
     3361 ccgtcagaac cgaagcccca gaacttgcag ctgatcgtcc cttccgtatc gacggcaata
     3421 gggatatccg gcatggagag atgggttacg tcatcgatga tgcccaaggt gaagtggttc
     3481 ttcggctgga gggcttccat gttgttgaat acggcgttga tggctgccgg cggaacgtct
     3541 ttcgacgaca agccatagcg acaggctaaa acagtaacgt cctgtttaat gtgagataca
     3601 gcagcgcaaa catcttcgta aagcggttcg cccaaggcgc ccggttcttt cgtgcggtcc
     3661 ataacggtaa tcgtgcgaac cgtatccggc atagctttca tgaagtgttc gagggagaac
     3721 ggacgataga gacgtacttg gaggaagccg actttcttgc cgtctttgct gagtttatcg
     3781 atcgtttcct ggattgcgcc ggtaacggaa cccatagcga cgacgacgtg ttcagcatcg
     3841 ggagccccgt agtaatcgaa gagatggtac ggacggcccg tcagttctcc gatctgatcc
     3901 atgtagtcct gaacaatgta aggcagacgc tggtaccaga ggttgttagc ttcgcgggac
     3961 tggaagaaca tatccgggtt ggtaacggta ctgcgcatga ccggatgttc cgggttgagg
     4021 cccgtgtgtt tgaaatcata aagtgctttg cggtcgatca atttgcccaa ttcttcggcc
     4081 ggcagcatct gaatcttttg aatttcgtga gacgtccgga aaccgtcaaa gaagtgcatg
     4141 aacggtacgc ggcctttgat cgttgccaag tgagcgacag ctgcgaggtc agcacattcc
     4201 tgaacactgg cactgcagag catggcccaa ccggtgttgc gacagcccat aacgtcagaa
     4261 tggtcaccga agatagacat ggtgtgagtc cctaccgtac gagctgcgat gtgcaggacg
     4321 atcggtttca attcaccggc cagacggtgc atgaccggga tcatcagcat gaggccctga
     4381 gatgccgtaa aggaggtaca gaggctgccc gtttcggaag cgccgtgagc ggcaccgata
     4441 gcaccggctt cggactgcat ttcaaccaac ttaacggtct ggccgaataa gttcttacgg
     4501 ccgttggctg cccatacgtc aacgtgttca gccatcggag acgacggagt gatcgggtaa
     4561 atagttgcga cttcggtaaa gttataggcc acgtgtgccg cagcttcgtt accatccaaa
     4621 gtttttacga tttgcttaat cataaagtac acttcctttt cttaaaatga acataaattt
     4681 aatccggact cgtataacgt ttaacgtttc tatgctttta gtatagtcgt tggagctata
     4741 aaagtcaagc atagtgttaa tgccatttta tcatcgatac gtgtatagtt atacgtttta
     4801 cgtaaacact acgtaaatag ttaaaatggt acgtccactc tggaagtctg ccgcagcagg
     4861 catatttatg ccaaaagaga ggaccttttg tgatagttat gtcacaaaag gtcctctctc
     4921 agtggatatt cgatttttct cgctttacaa agaagtacta ttgaagtcca ttagtaccaa
     4981 cgagtcatag gcaggtcgtt cttacttccc ttggtcatca agatctgatg aagatcgata
     5041 ttgccgatgt agaaggagac ggcgcagccg cagagataga gatcccacat gcgggcaaat
     5101 tcttcgcctc tggcagcgat gacttcgtcg cgaaccttct ggaagttgtc gaaccagcac
     5161 atcaacgtct tgtagtaatg acggcgcagg ctttcgacgt cgatgacctg gaagtcattg
     5221 tcataagcaa ggcttacgat ttcacggagc gacggcaagg ttccgcccgg gaagatgtat
     5281 tttctcatcc acgggttgcc gatgctttcg tcgtggccgc tgatgtagtg gagcaggaac
     5341 atgccgccgt cttttaagag gtggctggcc gtttccatat ataaaggata gttggaacga
     5401 ccgacgtgtt ccaacatgcc aacgctgacg atgcggtcat actggcggcc ttcggcaacg
     5461 aggtcgcggt aatcgacgag gtctatctga accttatcct gaaggcccat ggcttcaatc
     5521 cgttcctggc ctttcttcca ctgttcctta ctcaacgtgc aaccatagcc tttaataccg
     5581 tatttcttag cggcttcgat gaggaggtag ccccagccgc agccgatatc gaggagggtc
     5641 atcccttctt caagggccag tttttccaaa atatagtgga ctttattgtg ctgagcctgt
     5701 tccagtgtgt cgtcgtcctt tttaaagtag gcgcaagagt aactcatgga cggatcgagc
     5761 catttggcat agaagtcgtt gcctaaatca tagtgcgacg aaacctgctg cttctgagcg
     5821 ctcttcgatt ccgacggata gagcagacgc ttaaagaagg atttatccag cgagaactga
     5881 ctgttttgcg acaagaggca gcgcaaggcc gtgaataagt ctccttcgat ttccacatct
     5941 ttgcgcatat aggcttcacc gagagccagt gacgtcgaag ccaggagctc tttcttcggg
     6001 atgtctttgt tgatgacaat attgaacaag ggcgttcctt cgccaatggt ataagtctta
     6061 tcgtgaagtc gtacggtaaa acaatctcta tcgaagcgac gtaaataatg aactagaaaa
     6121 ttatctaaaa tattcataga tactcccctc tctgttataa aatagattct gtccgggcga
     6181 tgccgcagac gacgttttca agaatcatca tcgtcgtcac ggcgccgaca ccgcccggaa
     6241 cgggcgtgat ggctccggca cgaagagcgg cgtcttcaaa ggcgacgtcg cctacggtct
     6301 taccgtcgac gcggttgatg ccgacatcga cgacgacagc accgggtttt atcatatcgc
     6361 cggtgacaag gccggccttg ccagccgctg ccataatgac atcggccctt tggcagacgg
     6421 ccttgagatc attcgtccga ctatggcaga cggtcaccgt cgcattgcgt gaaagacaga
     6481 gctgagccag cggttttccg acaacatggc tgcggccaat gatgaccact tcttttccgc
     6541 tcagcgaaat atgataataa tcgaggatag ccatgacagc ctgaggcgtg cag
//
LOCUS       DAWDXZ010000031         6521 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3600_length_6521_cov_8.520878, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000031 DAWDXZ010000000
VERSION     DAWDXZ010000031.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3600_length_6521_cov_8.520878"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1553..1562
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3526..3535
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1846 a         1539 c         1729 g         1387 t
ORIGIN      
        1 gcctttcggc caggggttca aggatatgtc gccgccgtcg aaggagctgt tcaagctcct
       61 gatggaaggg aacatcatcc atggcggcaa tcccgttctc aaatggatgg ccggcaacgt
      121 ggtcatgcgc caagacccgg cggggaacat caagccggac aaagaaaaat ccgtcgaaaa
      181 aatcgacgga atcgtggcat ccatcatggc actagaccgc tgtatccgca acaggacagg
      241 cagcggcagt gtctatgatg aacggggcgt tatttccttt tagaataaat cactgtgact
      301 tcccgtacgg gtcagcgtca acgttagaat gtcggtctcg actttgtaaa tcagcaacca
      361 atccggcaga atatgacatt cacggaaacc agcgtagtct cccgacaggg catgatcccg
      421 atacttggca ggaagcaatt gctccatcac aagacagtca agtacttcct gaaaaagctc
      481 cggggtatgc ccaggctgct tgattacccg cttgtaatcc ttgcggaatt gtttgcggaa
      541 gcggatctta agcattcagg gcctccatga catcttcaac agaatcataa gtcttgctga
      601 gattgcgatg atgatttaca tcgtcaacag ctcttaaggt ctctagctca ttaggagaaa
      661 tacgcaaatc gaacgggata ccgccatagc ggacaacggt gcgtaggaac atattgattg
      721 ctgtagaagt cgggataccc aaatccgcaa aaatagtttc tgcctgatgc tttaaatctg
      781 catcgacacg aagattcagc gtttttgtat cagccatatg aatcaactcc ttttattgat
      841 attataaagc taaatgagga aaatgtaaag taatttactt tacaatataa ggacggagat
      901 attatgcaaa tcccattttt atccagcctg ttccgtacca gggacaagcc tcagaaccat
      961 tacatcggca cggatttcca ctatctcttt ggaccgtcaa cgagcggtaa gtcagtgaac
     1021 gagtttacgg ccatgcagac aacggcggtg tatgcctgtg tccgcatcct gtcagaaacg
     1081 ctagcagccc tgccgctcca gctgtaccgt tacacgcccg gcggcaagga gcggatttat
     1141 gaccatccgt tgtatcatct gctgcatgat gaaccgaacc cggagatgac ttcgtttatc
     1201 ttccgggaaa cgctcatgag ccacctgctc atctggggca atgcttacgc tcagatcatc
     1261 cgcgaccgat tagggcgggt acagggactc tatccgctgc gtccagacaa gatgaccgtc
     1321 tgccgggatg accggggaca gattttttat ctatatacca agacgggaga tgagaatccg
     1381 aacatcaagc cgtacgggca ggtggcactc cagaaggaag aagtactgca tatccccggc
     1441 cttgggtttg acggcctggt cggctattcg ccgattgcca tggcccgcaa tgccgtgggc
     1501 atgaccatgg cctgcgagga atatggtgcc tctttctttg ccaacggggc cannnnnnnn
     1561 nnagcggggt gctggagcat ccaggcgttc tgaaggaccc ggccaaagtc cgggattcgt
     1621 ggaatgccgt ctaccggggg acgggcaatg cccacaaggt agctgtgctg gaagaaggtg
     1681 tgcgcccaga cgggcgtcat tgatagtagt gtttggtact accacccaca atcatgggtg
     1741 agttgacctg ctttaccgca atgcgaaagc tgatacggga acatagcacg gcaggaaagc
     1801 ggtaagttac ccaaaggcta taaggtacga ctgaaccgca acaacaatcg gatatgaggt
     1861 ttaagttatc tactgaacgt gagacctgag tgtccatttc cgaggggaat tgggaaatta
     1921 gcctattacc cattccgtga ccggctgtct ttacatcctt caaagacagc atgattgcaa
     1981 atgtcacggc acgagcagga gaacctgtgt taaagggtct aaagcggaac cgacaatccg
     2041 agcataccaa gcaatgacgc taactgggga taccctaaag acggatgccg aaaggctata
     2101 gtctatagga cttgaatacc gcctatgggt acggagcgtt cgtagtagtc tgagagagtt
     2161 aatggctctt acatggcgaa ggaacgcagt ttatgcaact ctaaaaggaa agatgaaagg
     2221 gaggagaaac ctcgatgaag ccaacatctg aaattttaaa gcgaatgtac ggaaattctg
     2281 aagagcattc agacggtatc tacacgcggc tctataggta tcttttgcga gaggatatct
     2341 acatgaccgc atacaagaac ctttacggaa acaagggcgc aggaactgag ggtgtggata
     2401 atgatacggc tgatggtttt ggaaaggaat atgtgaatca gattattgat gaactgaaaa
     2461 acctgaccta tgagccaaaa acagtaaaac gtgtctacat tcctaagcgc aacgggaaaa
     2521 tgcgtccatt gggcattccg tcattcagag ataaactggt acaggatgcg atacggcaga
     2581 tacttgaagc aatctatgag cctgttttca gtgcccattc gcacggattc agaccaaata
     2641 gaagctgtca tacggcgctg aaagaaatca gtcgttcgtt ccgcagtacg aagtggttta
     2701 tcgagggtga tattaaaggg tgctttgaca acattgacca tgcggttctg ctgaatctgc
     2761 tttccgagaa gatcaaggat agcaagttca taaatctgat aggaaagttt ctgaaagcag
     2821 gctacatgga aaattgggaa taccacaaga catacagcgg aacaccgcag ggcggcatac
     2881 tttccccgat tcttgcgaat atatatctgc atgagttgga taagaaagta gaagccatgc
     2941 aacaggaatt caatgcgtct gctgaatatg cctatacacc tgcatacggt aaaaaggtaa
     3001 gagagattgt caagctgaga aagcgttacg gtgattgcga tgatgaagca gaaaagaaag
     3061 aactgctaaa acagattcat aagcttgaag tggaaaagcg cagattgcca tacaaggacg
     3121 cttccgacaa gaaaattgcc tatgtacgct atgccgatga ctttattatt ggtgtcagcg
     3181 gaagccgtga agatgcggag agcataaagc aggagcttac gttgtttgtc gcaacaaaat
     3241 taaaactgga actgtctgac gagaaaacaa aaatcacgca cagttccgac aacgctcatt
     3301 ttctcggata tgacatcaac gtgcgcagat gtcaggaatc caaaaggaaa accaacgggg
     3361 ttgtacaaag gacgcttaat aactctgtag aattgcttat tcctatggag cggattgaga
     3421 agtttatgta tgaccgtgag attgtcatcc aaggtaaaga cggcagtctc attccatggc
     3481 aaagaaactc gatggcaggt cttacagacc ttgaaattgt agatannnnn nnnnnctgta
     3541 attattacag cattgccagt aatttctcaa agctgacgta tttcgtttat ctaatggaat
     3601 acagttgcct gaaaacactt gctaagaagc ataaaaccag aatatcaggc ataaagagaa
     3661 tgttcaagtg cggtaaatca tggggcgttc cctatgaaac gatgaaagag aaaaagcgca
     3721 tgatgattgt gaaattctct aacttcaaac ggggaaaagt ctttgaagaa ccaaacattg
     3781 atacggtaaa gaaccatatt catttaaaca caagaaattc tcttgaagcc aggttgaagg
     3841 cttgtaaatg cgaattatgc ggtgcggaag gtgacggaat ttcctttgaa atccatcaca
     3901 tcaacagaat gaaaaatctt aaagggaaag agcaatggga aatggcgatg attgcaagaa
     3961 aaaggaaaac acttgtcgtt tgtagagaat gccataagaa aatccatcat tcgtaagagc
     4021 gtaaatggaa agccgtgtac atcgagaggt gtaagcacgg tttggggaga ggcatgtgca
     4081 aaccgacatt ggaaacaatg cacggcggca cttgcctact ctacatgaag taccagcaga
     4141 tcggcatccc gccggaagaa gcacagttcc tggagacgcg gaagttccag ctcgatgaga
     4201 ttgcccggct ttaccgcatc ccgccgcaca tgatcggcga cctggagaaa agttccttca
     4261 ataacatcga gcagcagtcc atggaatttg tgaaatacac ccttgacccg tgggtcatcc
     4321 gctgggagca ggccatgcag aaagccctgt tcctgccgga agagaagaag cagtatttcc
     4381 tgaagttcaa cgtgaacggc ctcatgcgcg gcgattacga gagccgcatg accgggtaca
     4441 gcatcggccg gcagaacggc tggctgtccg ccaatgatat ccgggagatg gaagacatga
     4501 atcccgtgcc ggatgaagaa ggcggtaatc tataccttgt caatggcagc atgacgaagc
     4561 tcaaggacgc cggggccttt gcccagaagg gagaaacgaa tgaaacataa attttggaag
     4621 tgggtgacca acgaagcacc ggattctttc ggcagtaatc gcacgctcta cctggacggc
     4681 cagatttccg acgaaacctg gtggggtgat gaagtgacgc cgaaggcgtt caaagatgaa
     4741 ctgaacgcag gcagcggtga catcacgctc tggatcaaca gcccgggtgg ggactgcttt
     4801 gccgccgcac aaatctataa cctgctcatg gattatccgg ggaacgtcac cgtcaagatt
     4861 gacggcctag ctgcttcggc agcttccgtc atcgctatgg ccgggaccaa ggtctgtatg
     4921 tcgccagtgg ccatgctgat gatccataat ccggcgaccc tggcctatgg cgaccaggca
     4981 gagatggaaa agaccatcgg catgctgagc gaagtcaaag agagcatcat caatgcctac
     5041 gaaatcaaga gcggcctggc ccgcacgaag atttcccaca tgatggatga cgagacctgg
     5101 ctcaacgcga agaaggctgt ggaactgggc tttgccgatg aaatcctgtt cgaccagaag
     5161 aagggaaatg aagaacagcc ggaagccatg ctctacagcc cggtcacggt gacgaattcc
     5221 tttgtacaga aactgaaacc aaagaaaccc ttgcagaaag tgccagccgc tttcctggag
     5281 aaccggctgg cattgctcat tcattaagga ggacaataat ggatacgatt ttagcactgc
     5341 gtgagaaacg caagaatctg tgggatgcgg cgaaagcctt cctggatacg gcccgtgatg
     5401 agaacggcat ggtgtctgcg gaagacgcgg cccggtacga caagatggaa gcggatgtag
     5461 taaatctcgg caaggaaatc gaccgcctgg aacgccagca gcagcttgat gcccagctgt
     5521 cccagccgac aacgatgccg attactgaac tccctggcgc aggccagaat ggagcagaaa
     5581 agaaaggctg tgcgtccgat gcctatcgta aggctttctg ggacagcatc cgccataaga
     5641 acttcattga tgtacagaac gccctgagcg ttggcacgga tgccgatggc ggctatctgg
     5701 tgccggatga attcgagcat cagctcatcg acaagctcca ggaagagaac ttcttccgcg
     5761 gcctggccac cgtcattcat accagcggcg accgcaagat tcccgtcgtg acgggacatg
     5821 gcgaagcgtc ctggatggaa gagaatgggc tctatccgga cagccaggat accttcggcc
     5881 agcagtccat cggggcatac aagctgggga cggctatccg tgtgtcggaa gaactgctga
     5941 atgacagcgc tttcgacctg gaaagctata tcgctggcga atttgcccgc cgtatcggta
     6001 cgaaggaaga agaagccttc ctcatcggtg acgggaagaa caagccgact ggcgtgttcc
     6061 cgtccgcgga gctgggcgtg acagccaatg gcgcatccat cacctttgat gatgtcatcg
     6121 acctgtatca ctccctgcgc atcccgtacc gccgcaaggc tgtctggctc ttgaacgatg
     6181 cgaccatcaa ggccctgcgc aaaatcaaag acaacaacgg caactacatc tggcagccgt
     6241 ctgtcacggc cgggacaccg gataccatcc tgaaccgtcc ctgctacagc acatcctttg
     6301 ccccggaact ggcggccggc agccgtccca tgctcttcgg cgatttcagc tactactgga
     6361 ttgccgaccg ggaataccgc tccttcaagc ggctcaacga actgtatgcc gccaacggcc
     6421 agatcggctt cctcgccagc cagcgcgtcg atggcatgct gatgctgaag gaagcggtcc
     6481 aggccctgga gatgaaagcg aagggataag ccatgattgt g
//
LOCUS       DAWDXZ010000032         6498 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3608_length_6498_cov_9.415800, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000032 DAWDXZ010000000
VERSION     DAWDXZ010000032.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6498)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6498)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6498
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3608_length_6498_cov_9.415800"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2986..2995
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5696..5789
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1411 a         1751 c         1728 g         1504 t
ORIGIN      
        1 atgaatggct gcccaaatga ggcgcagcgc aaaaggcgaa atgccgtagc ccacgagggc
       61 cccgagaatg atgccgacaa tataagtcat gacggccatg atggtcgtcc caaaaatact
      121 gccgtggaaa aattcccctt cgatttctgc actgataaac ggcgtcccga taagctggtc
      181 ggcaatcatg acaaaaatga cgaccatgat caggatgatg acgcctttca tgatattatc
      241 agctacaatg cgggcattaa acttcttcgg tttccgttct ttatagtctg aacccgtttt
      301 atcttccata cgtaaaaaga cacctctctc tccacatctc aactgtaact acactagttt
      361 accacatttc cggacaatgt accacaattg ccccgtaaag tttttggttc tgaaggcagg
      421 cggcacctgg ctcgaccgtt tcactgcctt caaaggttaa aaggtaacgc caaaaatgcc
      481 atgacgaccc gttcatgaca tttcagcaac agacaattat tcacaaagat aggtgtagag
      541 tttctcttcg acaggcttat cgatgacaaa tcgaaaattg acgatgggca gctgcacgcc
      601 ggcatcgttg gcaatggtcg tctctacggg atcgccgagg acctttaaat cgccgatata
      661 gtagaagttt gacccttcgg catcgctctt tttcacgaac agcagcttcc gcgtacggct
      721 gtcctgaata gccggaatct gggaagaggt caggcgaacg cgggcccggg tcatccaact
      781 gaactcctgc ggggatatga atcggtcttc gtacttcgta ttggctgaaa tatcgtcaca
      841 cttttcatag gtaacgaaga tgggacacgt atgtccgacg atttgatagc cgttcagcgt
      901 cccttcgcgg ttcgtatcgt aattgagaat gcgcgatacg tcttttcggg aatagcgtcc
      961 atagcgtaca aaaccgccga cgatatcggg atcatcttga atatacaggg attcaaagcg
     1021 atacgtccca taatcgagga catcctgggc ataggcctta aattcggcgt tctgtttcaa
     1081 ggattcaaaa tacggcgtcg accgatagac atggttttca aaagaaagga ggggcaaacc
     1141 tccgtatttc tgctgcgcct ttttgccaaa aaattgcaga cttaataccc gggcagcatg
     1201 gtccatcgtt tccgtcgaag gcaggaatcc gtacaatcgc tccatctctg ccgcaaggtc
     1261 cgccgtcgac cacgacggtt cggccaggac atgacgaagg atcaggattt cttcgatgcg
     1321 cttcccattt gaaagttcta ggctcatcag cttcagcacg gaccggtggg cctcattcag
     1381 cgtcgacggc tgcggctcca tatactggga aaattcatag aaggactctt ttttggcaat
     1441 gaacagcatc ggatccttat cgccaaaacg gacgaaatcc atcatcatgg gctctctgcc
     1501 caaacggcat ttcatgtcgc gataggcgtt tttcaagtcg tctaaatcgg ctaaagcctt
     1561 ctttttgtcg atggccgcat agattcgctc cttggcaatg tcgtcgaagt ggaccgacgt
     1621 cggtccgggc aggaaattgg tctgcatcag actgcggaca aaatccttat cgtaggtccg
     1681 gtcgccaaag agggcaatgg gcagcaaaaa gttattttca tagttgccga tgaagtcgag
     1741 gacttccaaa taccgcttat agggatactt tcgaaggccg cgccccagct gctggacgaa
     1801 gacgatggcc gatgccgtcg gacgcagcat gatgacttga ttgacctgag gaatatcgac
     1861 gccttcatta aggatatcgc aggtaaagat ataatccaaa tagtcaccgt cttcttcggc
     1921 cctggcttct aaatgaccga tggcgtcctg ccgttcacgc tctgtcgagg cgccggtcaa
     1981 ggccacggcc cgtcggccgt gtttgacaaa agcagccgcc agggttcgcg cttcttcgac
     2041 gctgcggcaa aagacaaggc ctttgacgcg gtccgtatca ctgccgtaga ggtcggcata
     2101 atggagaatg tgccgcaccc gttcttcaca ggtcagggcc gtaaaatcac tgttgtcatc
     2161 gaggacctcg ccattgaccg tgatttcgct gacaccatga taatggaagg gcaccagcat
     2221 attttcggat aaggcgtcgt gaaggcggat ctcataggca atctggtggt gaaacaaggc
     2281 gtaaatatcg aaatcatcgg accgttccgg cgtcgccgtc atgcccaaga ggaacgttgg
     2341 cttaaagtag tcaagtacct tctgatacgt cgccgctccc ccatgatgga cttcatccat
     2401 gacgatgtag tcaaaggcat cgggagcaaa ggaatagagg atgtcatcac gggataaggt
     2461 ctgtatcgtc gcaaagagat aaggcttgtc gacgtctttg tcgtgccctg tcaatagacc
     2521 cgtgtcggcc ggatcgacgc cgactttgac gtagctggca cgagcgcttt tgacgatgag
     2581 ctcgcgatgg acgacgaaga ggaaccgccg cggcctcagt ttcatgacat cgaaggccga
     2641 cagataggtc ttgccggtcc ccgtcgccga aatcaacagc ccccggtctt ccccctggtc
     2701 acgcagcctc tgaatgcccg cgagggccgc cttttgcata gcattgggct gaatcttatg
     2761 aaaaggcaga tacaagcccc tttttctcgg attctggcgt ttataaatgc tttgataggc
     2821 ctggatccaa tcttcatcga cgacttccgc cgtatcccat aaggcttgaa attcctcttg
     2881 tgtctgacgg atgagggccc cgttttcagc cgacgtaaaa aagacgttcc attcctgatt
     2941 gtgcgtcaag gcattggccg tcatattgct gctgccgatg accatnnnnn nnnnncctgt
     3001 gattttcgat gagggcatcc ttcaggcagg caatgccgct cttggtcaga aaggcgacag
     3061 aaaacagaaa ctggtcgcag acgtagagct gcgacaagat ggtgtcgagg acctttcgtt
     3121 tcttttcttt attattcgtc aggattttcg gcacatagcg ctggtcggct tcgatggtat
     3181 agtcgataaa gccttcgcgc aggcactgtt caagggctcc gtattccgcc atgactccgc
     3241 ctactttccg gctaatttaa gcatgatcgg cacgtccgcc ggagcccagt ccaaggtcgg
     3301 cagatctttt ggcgccaacc acttggcggc gacgtgttct tttcggatga aatcaggatt
     3361 atccagccgg cagtaaaagg catggaggct gatggaaaaa tcgggatagt cgtgatggac
     3421 ggtcatatag aggtcttctt cccggaccga tacatggacg tccatttctt cccgcagttc
     3481 ccgttcaatc gcttcgtgct tcgcctcacc gggctcgatt ttcccgcccg ggaattcata
     3541 cttaaagctg acgtagtcga acttcccctg tcctcgttcc atacagagga tctttccgtc
     3601 gtattctaaa atcgctgcca ccacttccaa atgcttcatc acacgtcctt ctttcctcgt
     3661 ttttccctat tttaccatgg taaagacttt ttgcctactg aaagccggct tttcccaaca
     3721 gaatcgggat gatccgccaa aatcaagcaa aggccttttc aatagcctcc cgaagggtgg
     3781 ccacgccgac gaagcggcag tcctccggaa aggcaccggg atcgacgtct tcgaggttcc
     3841 ccttcggcat gataaaggtg cgaaagccca ttttcgccgc ttccttgacg cggcgcaggg
     3901 cctggggcac gcggcggaca tcgccggtca ggccgacttc tcccatgacg acggtccggc
     3961 tgtccatgga gatgtcgcgc aggctggaat agacggccag gaggaccggc agatcggctg
     4021 ccgtttccgt caggcgcagg ccgccgacga cgttgatata gacgtcctga ctgccaaggg
     4081 cggcaccgag gcgcttttca aggacggcca ggaggatgat gaggcggtta taatccatgc
     4141 ccgacgccgt cctccgggga acggcaaaga ccgagtgggc cgtcagggcc tggatttccc
     4201 ccagcatggg ccgcatgccg tcgaggacgg cggcaacggc agagccggct tggtcggccg
     4261 cccgttccgc caggaggagc tgagacggat tttccagttc tgacaagccg ccgccggtca
     4321 tggtgaacag gcccgactcg ttggtcgagc caaagcggtt cttaatgccc cgaaggatgc
     4381 ggaactggta gttgcgttcc ccttcaaaat agaggacgac gtcgaccatg tgctccatca
     4441 tgcgcggccc ggcaatggcc ccgtctttcg tgacgtggcc gatgacgatg accggaatgg
     4501 cttcactttt ggcaaaggtc ataaggcggc tcgtcccttc gcggatttgg gataaactgc
     4561 ccggcgtccc ttcgacagac tggctgtaca tggtctgaat cgagtcgacg atgaccaggg
     4621 ccggccgccg tttatgggct tcacttaaga tattgtccaa ggacgtatcg gcctgaatga
     4681 cgagattcgg cgcggcaatg cctaagcgct gagcccgtaa ctttatttga ccggccgact
     4741 cttcgcccga gccgtagagg acggcgccgt tcttatcaca gacggccgcc gccaattgga
     4801 ggaccagtgt cgatttgccg atgcccggat caccgctcaa gaggatgagg gctcccggca
     4861 cgatgccgcc gccgaggacg cggtcgagtt ctccgattcc cgtttcgacg cgggtcatat
     4921 cttctaaggc gatgtccgac aaggaggtgg cccgcgacgc cggtccgctg cgggccatgc
     4981 tttgggccgc cttaggggcc tcaggagcca cctgttcttc tgccatcgta ttccactcgc
     5041 cgcactgagg gcagcggccg agccagcggc tggaaacgct gccgcagctg gtacaaacgt
     5101 aacgagtttt tgcttttgcc atactttctc ctttgcactg tgttttagtg cgattcataa
     5161 aaaaggcttc gcatgacaag cactgccttc atgcgaagcc ctttcatcat tctgtcgtaa
     5221 agacgagctt cttcttatct aagctgtcga cgtgaatcgt attgccgtct ttgacgtcac
     5281 cgcttaagag catttccgaa atcgggtctt cgaccagttt ctggatagct ctcttcagcg
     5341 gacgggcgcc gtaggcaaaa tcggttccat ctttgacgag gacgtcatcg gcggccgtcg
     5401 tccagttcag gcccacaccc ttttcgccaa gacgcgccgt cagctctttg agcatgatat
     5461 cgacgatctg acgaagatcg gactgagcca ggggcttgaa gacgatcatt tcatcgatac
     5521 ggttcaagaa ttcaggccgg aagaagcgtt tcacgtcctc gaggattgcc tttcgcgctt
     5581 cgttgaaagc cttttcttca ctgccgtcac cgtcaccgcc ggtagcaaag cccatgaccg
     5641 gaccggacgg acggagatgg gaggccccga ggttgctggt catgatgatg accgtnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cgccgaccag gcgggagacg ctgtattttt
     5821 ccataaattc gctcatgtcg atacggatca tgttgtcggg gctatcgaag agttgggccg
     5881 ctaaggtacg ggccaattcc gtcttgccga caccggacgg gccgaggaac aggaaggaac
     5941 cgataggacg gtttgcatct ttcagtccgg cccgggcacg gcgaacggct ttcgctacgg
     6001 ccgtaacggc atcgtcctga ctgatgaccc gcttatgcag ctcttcttcc aagtggagca
     6061 ggcgctgcga ttccgtttcc gtaatctgct ggacggggac gccggtccac atggaaacga
     6121 cgtcggcaat gtcattttcg gtcacgacga gtttttcatc gtcatgctgc ttcttttcct
     6181 tctgcagggc cgaaatttcc gaagcgattt ttttgccggc atcgcgcagg gcggcgcatt
     6241 tttcaaagtc ttccccatcg agggcggctt ctttttcctt cttgatatcg gccagcttct
     6301 gttccaggac ctgcagttca tgagacggcg catagacttt catgcggacc cgtgccgtcg
     6361 cttcgtccat gacgtcgata gccttatcgg gcaggtagcg gtcggaaata tagcgggccg
     6421 acaatttgac ggcagcccgt acagcttcat cggtgatacg ggccttgtgg aaggcttcgt
     6481 accgatcgcg caggccga
//
LOCUS       DAWDXZ010000033         6447 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3629_length_6447_cov_9.307103, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000033 DAWDXZ010000000
VERSION     DAWDXZ010000033.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3629_length_6447_cov_9.307103"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1619 a         1724 c         1591 g         1513 t
ORIGIN      
        1 ccgatagtcg gtgtgaatcg gatctaccca ctgagggaag tattccgaca aatcagtgtc
       61 acgaagaagg ccgcctgttt cgatggacca gcgaactata tcttgagcca gttcgccttc
      121 ataaagggag cggcaatgac tgtcccgcag gagttccaac gagttcgcaa gttccggcag
      181 ggtcaggata tctcctgctt ggagcgtcct tcctcgggga aagaaggtat caaacagggg
      241 gcgaaaaagc ggatcctccc gataatctcg aaaggcgtcg gcactgtaag ccagcatggc
      301 agccaaatta ggcatgacca caaacccttc ccgagcatag gtgatggccg gttcaaacaa
      361 ttcatgaaac ggaagtttac caaaacggcg atgcacttcg gcccacgtcg caggtgcacc
      421 gggcaccatg accggatacc agccgcgcag tggcatttcc tcatatcctt cagccactaa
      481 tcggtctcgc gtcagggctg ccggagacca accactgcca ttgataccgt gcaactttcc
      541 atccatccaa aggagggcaa aagcatcgct ccccaaacca ttgctggtcg gttccaaaac
      601 ggtcaggcaa atggccgtcg ccaaagctgc atcaacggcg ttcccgcctc gtttcaacat
      661 atcgaggccg gcctgggcag ccaaatgctg agacgtgcag accatgcccc ggcggccgta
      721 aactacctct cgatgagatc cataagggta gacagtacca tcaaaattca taagacctta
      781 ctccttttct aaaaaatact aagcggaaac ctatctaaag aaagacttcg acaaaattca
      841 gacaaatcct tgtccgccac cttcacgaac ggcttttttc tcgttataat agtaattgca
      901 tcatgcattt cgagaagaat ttacaaggta aggagtagaa gcaagttgaa acggtgtatg
      961 ttaatcatca accctacagc agggcgtgag agagcaaaat accacaaaga aaatttaaga
     1021 cgccaattgg agagcatgtt cgacgacgtg gaattacgag aaacagagaa ggcaggagac
     1081 gccaccctat gggcaaagga agctgccctg atcggattcg atgccgtctt ttccatgggc
     1141 ggtgacggaa ccctcaacga aacgatcaac ggactggccc aggccaataa accgatcgac
     1201 ttcggattca ttcccttggg caccatcaac gacttggcca gagccttaaa catccccctc
     1261 caccctgaag tcgctattga tatcctgccg cgctgcaaga ccgtcaaagt cgatattgca
     1321 aaagccaatg accgctactt catcaatacc attgccaccg gaatcatgcc cgaagccgtg
     1381 ggccacgtat ccatcgagca gaaaacccgc ctcggcccgt tggcttattt tctgaccggc
     1441 atcaaagcca tgcaggccca tgaaacatct ttatttaaaa tcacgacgcc ggaaggttcc
     1501 tccgtctacc gttcgcccct catcgtcgcc atgctgacca actctgtcgg cagtttccgc
     1561 aatctggcac cccaggcgcg agtcgatgac ggaaagattt ggctgggaat ctttaaagat
     1621 ttcaactacc tggatttaat caaggtcatt ccggaatttt tatccggaca gcccttgacc
     1681 tcggaattga tgaccttaaa agcattggaa gaagttcgca ttgaacttct gagcgatcat
     1741 cccctttcga ccaatatgga cggagattcg gggccgtctt ttcccctcga tatcaaggtt
     1801 cttccatcct tcttatcggt ctatgttccg gagtaaacaa gcaaaaaagg agtttacatg
     1861 tttctaaaag gaaattttct ctatacccca tcgcaagcag aattcatcat ccttgaaaat
     1921 caatatatcc aagtcgaaga cggtaaggtc ctgggggttt tccaagacct tccggctcat
     1981 gcagcccctt catcggtcgt cgattacgga aaggacatca tcatcccggc ctttatcgac
     2041 ctccacatcc atgcgccaca gtacatcaac cgcggcttgg gctttgacga agagctcctg
     2101 ccttggctgg aaaactatac cttccccacg gaaggccgct tttccgacac ggactttgct
     2161 gcccgtgttt accgtgcctt cttgtcgcgt ttaaaagccg aagggactct atgtttttcg
     2221 gcctttgcca ccattcataa ggatgccact tggaaactca tgcgcattgc cgaagatatg
     2281 ggtttaaaag gatacgtcgg gaaagtaaac atggacagaa acagcccgga ctatctaacg
     2341 gaagatacag aacgctcttt agccgatacg gaagagctcg tcctccgcag ccgcgaagaa
     2401 ttggaccgta ttcgcttcat cgcaacgccg agattcgtcc cgtcgacgac agaaaagctg
     2461 atgatcggcc tcggccgcct ctgcgaaaca tacgatcttc ccgtccagtc ccacctgtca
     2521 gaaaaccgtc acgaagtcgc ttgggtcaaa gaactgcatc cggaccttcc gtcgtatact
     2581 agtgtctacg atgccttcgg gctgcttcgc ccacataaaa cgctgatggc tcatgccatc
     2641 tatctgtctg acgaagaaaa gcgtttactc aaagaaaaag acgtttactt gacccactgt
     2701 gcccagtcca atgcaaatct gaccagcggc atcatgcccc tccgcgaaaa tcttacgaag
     2761 gggttgacct gcaccattgc cagtgacgta gccgccggcc ataccccggc catgaacaag
     2821 caaatcgcct tgaccatcga aatttcaaag ctctattcac tgaatcatcc gtcagaaaaa
     2881 gccctcacca tcggtgaagg cttgtacttg gccacgaagg gccccggccg tttttatgga
     2941 aatacaggca gtttcgaaac aggcttcaat tttgacgcct tagtcatcca catggacgat
     3001 gatatcgaag gactggaacg gacccccttt gaaaagcttc agcaattcat ttacgacggt
     3061 gacgaccgca acatcgtcgc ccgttatgta aacggaaaga aaatctaatc gcaaaaaaaa
     3121 gactgtcgct gagcgacagt cttttatatt attcagccgt gtacggcaag agtgcgatag
     3181 cacgtgcgcg tttgatagca acagtcagtt tacgctgatg tttagcgcaa acgccggaaa
     3241 tacggcgcgg caagatttta ccgcgttctg ttgtgaaacg gcgaagcttt gcagtatctt
     3301 tgtaatcgat cagttcagca tgatcaacgc agaagctgca tacttttctt ctcggttttc
     3361 tcgagcgatc tcttctcatg tatagtttcc tccttcaaga attagaaggg gcgtgtctga
     3421 ccttttagaa tgggatttct tcatccgtgc tggagcccat gctgtcaaag ccggcaccgg
     3481 cagcagccgg tttcggctgc gctacactgg caggctgtgc cggagcagca gcttgctgat
     3541 gttctgatcc cattgtctgg gcaacaaagt cgcaaacgac ttccgtgcga taccgtttct
     3601 gaccatcctg gccgtcataa gaacggaccg agatacggcc ttgaacgaag acacggctgc
     3661 ctttggttaa gttattgcca cagttttccg ccaaattacc ccaagcaaca caaggaataa
     3721 aatcagttaa ttcccgcggt tctgaactgc cggtcggaac atacgaccgc gtgcaggcaa
     3781 cggtaaacat agcgacagcc cgtccggtct tcgtaaaacg gatttccggg tcccgagcca
     3841 aattccctaa taattgtact gagttcatac ctttgcgcct ccggttattc gtcgatcttg
     3901 acgatcaagt gtttcagaat agtatcgtgg atcttgatga cacggtcgat ttctttgatg
     3961 acttcaggtt cgccttcaaa gttaatcaaa acgtagtagc catcagcctg tttcttaaca
     4021 gggtatgcca gatgacgttt cccccaacga tctacctttt cgatactgca gccgttcttg
     4081 gtaagaaggt tcgataccaa ttcaacgatg ggatctactt ctgcttcttc gaccggtcgt
     4141 gcaataaaca tgacttcgta cttgttcacg aaatcacctc ctcttttgga catatgaccc
     4201 ttcccgaaga agggttagga gcttgacaca aatcaagctt tttaattata tcacagacct
     4261 atccggaatg caagaaaaaa gacagtcttt ccgccgtcaa aagcaggaaa gactgtcttc
     4321 ggttacattt atttaaagta gtttacgccg gatgtgaaga tgggttggag cttattgccg
     4381 tcaatgtttt taaagacatc gtcggcgctg aaccgttccg tatgagccat cttgccgagg
     4441 acacgaccgt caggactggt aataccttcc acggccagga ccgactgatt cgggttgtat
     4501 tcgctttcat aagaagcttt gccggaaagg tcgacgtact gcgttgcaat ctggcctttg
     4561 gcggcgagtt cacgaatctg ttccggcgtc gcaataaagc ggccttcccc atgggaaata
     4621 gcgacggtgt gaatatcacc ggctttacag ctgctgaacc aaggactcat gaccgaaacg
     4681 actttggtgt cgaccatacg ggacaggtga cggccgatgg agttgtacgt caaggtcggg
     4741 ctgtcggcct tcaaaggctg aatcttgcca tagggcagaa ggccgagttt aatcagagcc
     4801 tggaagccgt tgcagatgcc gagggctaag ccatcgcgct gatacaagag actttcgagg
     4861 gcttcggaca gggccggatt ccggaacaag gtagcaatga acttgcccga accttccggt
     4921 tcgtcaccgg cactgaagcc gccggggaac atgatgatct gtgctttggc catgcgtttt
     4981 gccatttctt caatcgattc ggccaagtcc tgcggcgtgt tattgcgaag gaccatgaca
     5041 tctgtcgttg cgccggcccg ttcaaaggca gctgccgagt catattcgca gttcgttccc
     5101 gggcaaacgg gaataaagac gtgcggagcg ccaaaggctt cactgcggcg aggaccatag
     5161 gtcgtaaaca agggcagttc ggcgtcgcct tggccatcct tatccttaat cgggaaaatc
     5221 tgattcagcg gtttttccca agcttcctga aggtcactca aggcaatcgt tacgccgtcg
     5281 gccgtgatat ttgccgacgt ggtcgtctta ccgaccaggc tgacctgatc gcgtttactc
     5341 catgcagcag ccgttgtact gtcggtttca actaagaggg cgccgtaacg gaggttaaac
     5401 agttcctgta cagcaaagtc tgcatcgacg tcgaatccga ttcggttgcc aaaggccatc
     5461 tgagcgacag ctgctgcgat accgccttga ccgacggcat gcatcgcttt gaccttaccg
     5521 tcaatgacgg cttgatggag gaagtcacaa tgctgtttaa agatatcgaa gtccggcatc
     5581 ccttcgccgt cacgcggcaa atcgaagagg aggacggcat cgtcagaagc cttaaattcc
     5641 ggcgatacga tatgatcggt atgttccgga gcaatggcga aagaaacgag ggtcggcgga
     5701 acggtgaggt gttcaaaggt accgctcatg ctgtccttac cgccgatggc ggcgacttcc
     5761 atgacctgct gagcgacgaa ggcaccgagg agggcgctga ccggctgccc ccaggccgtt
     5821 tcatttttgc ccaggctctg gaagaattcc tgcatcgtaa ggtatacttt cttacggtcg
     5881 ccgccgaggg caacgagttt agcaatggac tggatgacgg catagagagc gccgtggaag
     5941 ggactccaaa tagcgaggtc cggatcgtag ccgtgggtga aaatgctggc cgtcgtcgta
     6001 ttcccgtgtt ctacgggaat cttagcgacc atcccttcga ccggcgtctt ctgatattta
     6061 ccgccgaaag gcatgaggac cgtgcgagcc ccgatggtgc tgtcaaagcg ttcggccaag
     6121 ccttgttccg aagcgatgtt caaggttccc atcgtatcga gccatgtctt agcgacgtct
     6181 tttacatccg atgccttgaa gaaatcctta tcttccggtg ccgtgaccaa agccttgcgc
     6241 cgctgagtaa cgccgttggt atcgaggaag gcacgggtaa tattgacgac gttctgattg
     6301 cgccaatgca tgaccagccg tttcgtatcg gtagccacgg cgacaatcgt cgcttccaga
     6361 ttttcttcag cagcatattt catgaattta tcgacatttt ccggggctac gacgacggcc
     6421 attcgctcct gagattcaga aatagcc
//
LOCUS       DAWDXZ010000034         6407 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3645_length_6407_cov_7.786052, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000034 DAWDXZ010000000
VERSION     DAWDXZ010000034.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6407)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3645_length_6407_cov_7.786052"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1788..1797
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2284..2293
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3615..3721
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1580 a         1681 c         1445 g         1574 t
ORIGIN      
        1 gaagaaaatg cgatgacgac acctttcttt tccggtacgt actgtgccca atgcaggtgt
       61 ttgtcgacga tatacttaac cgtttccggc gtacggaaga cgaaaccgtg gagcgggcaa
      121 atgatatcga tggggagctt agacgctttt ttaaggacgt tcatgacctg cgggccatat
      181 tttccgacga tattgacata gtagcggcga gcttcatcga ggtaattttc ttcaaaatca
      241 gcctggtcag cgaagacgtt gccgacggta ccgaagtggc cgaaggcatc ggcactgaac
      301 aggacgtgat ccgttacatc atacgtcatg atgacttccg gccagtgaac catgggggct
      361 ttgatgaact gcagggtgtg cttgcccaat ttcagctgat cgccttcgtt aacggtcatg
      421 tagcgatccg gcataggatg gcggaagaac tgttcaaaaa gcttaaaagt agacggctgg
      481 ccgacgatct tggcttccgg gtatttttcc aagagagcca aagccgagct ggcgtggtcc
      541 ggttccatgt gctggagaac aaagtagtcc agtttgcggc ctttgagcaa gtattctacg
      601 tcttcaagaa attcgtcacg gcacgattta tccatagcat cgatgaggca cgtcttttca
      661 tcatcgatga aataggtatt atacgtaaca ccctgaggaa gggggaagat ccgttcaaac
      721 aattccgtac ggaaatcgtt gcatcccacc caatagatac cgtttttaat ttcttctaca
      781 caatgcatat atgtataacc tccttaaata gtagcagcac agctatgctg taatgtcaat
      841 tatcacgttc ctatcatacc atcttttcga taaataggaa atactttaaa tgaaaaaact
      901 taccaaaaga tatctattct ttattagttc tgtccgaggt ttgggcagaa tcgctgtcag
      961 aagtcgattc acgttcgccg gactgacggc tcttcgaggt atcggagcga gtatcactat
     1021 ccgattcttt ttcatcgctc tgagattcgc tgcggctctt ctgttccgat tcagaggcat
     1081 cactgcgctg ccgctcccgc gtcgtactcc ggctcggtac tggtgctgaa tcaccgattg
     1141 attcagcgtc cgatacgaca ctgttgataa gctgttccag tacgtcccgt tcagcctggg
     1201 acaaatcgtt atttccgctg agcatatccc gcgcctgctg agctaagctc tgagcctgcg
     1261 aacgagccga actgccgctg acagccccat tgcggtactg ctgtttcaaa ctgcgcagtt
     1321 catttgaaat ttcattggcc ttgctgcgaa tacgggccgc tgccaattca ccactgcgct
     1381 gtttccgagc gtccgcttcc tgcatctttc gatcttcttc acgattttcc aacagttctt
     1441 cctgctgctg ttcggccact ttatcaagcg atgctgtcgt aacatgagtg ccgacgtagt
     1501 attcataacc catgtacccc aaaatgacga tgggaacaca gagaagcatc atttcaaacc
     1561 ggcgcatctg ggctgcccgc ttccagctga tgacgctttt gcggcgatgt tccataataa
     1621 ggacgatgat taaaatcagt ccgatcaaga acaaggccat ataaatgaag agtttatgcc
     1681 aaaccgtcgc ctgcaggctt atcgtagacg gctgggaatc cgacagctgc cacaaatagg
     1741 tactggcctg atcatcggct ttggtgctgt tgctggtaac ctgcgtcnnn nnnnnnntat
     1801 cattactata atccatgatg gtataattgc gcttttggaa ctcatcggca tcggccttca
     1861 agctctgctg cccttctgcc gtagccgaca ctttcaaaac atcctgcgtt tcactgccat
     1921 cgtcatgaat gatggtctgc gcccgaaatc ctgataagag gaaaaatgca gccaacagca
     1981 taacggtcat agccagtttg gcaatctttg aatacccttt cataaactcc tcgctttcta
     2041 tcactcttct tttggcggta ccatcccgga atcgacgaga tgcggcctat caaacagaga
     2101 acgtccggcc ttatccgccc gagctttgtc catagcagcc gcgttactga gctgctctaa
     2161 cggaacgccg ttgacaaaaa tcccttcgat gacgccttct ccccgcttga tgcggatact
     2221 ggcgatgata ctgtcatcgg tgcgaaggct gtagataccg tcagccattt ccggagacag
     2281 gcgnnnnnnn nnnttccggg aatacaacgc gaacataatc ggtcccaaag gtagagattt
     2341 ccagccgcgg cacagcacgc cactgataag gaatcccgtc gacagaaata ttatgccagc
     2401 gccagccgac ataagccagt gcggcaaatt gaatcaatac gactaacccg aagacaatcc
     2461 agcgatgacg gccttccggc gaaaaagaaa gatgtatgcc ctgcaaaggc ggtgaaaatt
     2521 tcattccgac ttgcctcctt gatgggacca tggcgactgg gtaaagtgtt ttttgcgttt
     2581 cggttccggt ttcatatgac tccacggcga ctccgtaact gccggagatt tttctttggc
     2641 cttttcctgg gaaacggcca tctgctgcgg cacggcttcc gtcgtttcac gaataacctg
     2701 acgaggcggc acgaagtcat tccccgtgct ttcagtcggt acggacaggg taaataccat
     2761 cggggaatcc gtcgtattta ccgggacgga accggccttg gacgaattcg tatcagacga
     2821 agcctcaata cctgattcgc ccagcccgag ttcagccgga gacggaatac ggatatcctt
     2881 catccattct tcgaccgttt catccatttc gttttcctca ggagaatacc acaccggcat
     2941 ttcttcttct ggagcctgtt cttctgtagc tgacgcatca tcatttcgga cgcgtgccgc
     3001 cgaatggcga agtcttgctc ttcgtgaagc tctctgtgcc gctgattttt tatctttctg
     3061 catccgcctg aactggaata cgattaaggc caagacgata atggccaaaa tcggcgccag
     3121 cggcgaaaaa tgcgtcccga aaacgaaggt caggactacg ctgaccaaaa tgacgatgcc
     3181 gaatttaaga tgagcctggt gatcgggctt aaatccctga gccgtttcat agacaccgag
     3241 aacaacgatg tacaaggatg aaagaatgag gggaatggac gaataaaggg ccgatcgaac
     3301 gagaatcgtc tcaaaagcca ggatgacggg aatcaccaaa tacaaagccg ggctccacaa
     3361 ccgcttcttc acggttttcg ccatcatgcc tgccatgaat acaaagagga ggaccgtaac
     3421 caaatcggtc gtccaatgaa aattctggac ctgagcaatc ccatcccagg caaagggcag
     3481 gcaggaaatg aggagcgaac cgacagcggc aaaccgcccg aaccaacgga agggcacgcc
     3541 ccaaggccgg tggatgtcga tggaataacc taccagcatg attgtaacgg ccaagctgcc
     3601 caaaaggagg aatgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nagccgcaac agagcataaa aaatagacga caggcagaca aagcacggca caagtaatga
     3781 catatgacgc cacatcccaa ccgatataaa aagtctgttc agccatggct aatacaacaa
     3841 aaatgcccaa acaatggatg agcgcaatcc cttcagctgc cgccgagccc tgccgtttct
     3901 gaaacatggc caggcctaca ccggcctgag tcacgagcag cagcacaccg gccaaggtca
     3961 tgcgggccgg gaccgacacg tagtaccata attgggaaag gccccaaatg acggccatga
     4021 caacggctgc aaatcccagt gccgataaaa acgcatataa aggcagggtc gtcaaaagag
     4081 aatttctttg ttcatccctg ccgtaagatt tccgtatttt ttcacaggct tcttctgtaa
     4141 tccatccttt agaaacccag tccgtgacct tgtggtctag ccattcattg gtattccgtc
     4201 tcatacttgc ctcccaggcg ccatcccgga acgaggcact ccgggaccct tcgtaatgtg
     4261 atatttcagg cattcctaaa aaagcacacc tgtctattca gtatatcata aaatgactct
     4321 tttccctatg gcatccgtca aatttttaca tattcccaaa agaatacctt cccctcatcc
     4381 gttataacca aaccaatccg caaaaagcca tgacggcacg aaaaaagggt ctccagggat
     4441 aatccctgcg agacccttta accagcctgc ggcagaacaa ggccatgaag gcattgcgtc
     4501 accgttattt tacgatcaat acggggcaga ccgaatgcgt gacgacataa ctgctgaccg
     4561 aacccatgaa taagcccttc agcggtccca ggccgcggct gcccatgaca atcaaatcgg
     4621 cattgtactt cttcgctacc gacaagacag caggccccgg cgagccgact tcaaagacgc
     4681 tcttgacttc gatgttttga ggaatcgcct tggcaaactc atcaaggatc cccttccctt
     4741 cttcttccat gtccagggca acctgttcgg ttacgtagcc actggcattg ggaatctggt
     4801 caaaattcga gatgaccgag acgatgttgg caacatagac cagcgtaatc atagcgccca
     4861 aagatgatgc aatgacgacg gcatgttcca aagctcgttt cgagttgacc gaaccgtcgg
     4921 tcggaacgat gatgttatta tattgcttca tatgaatccc tccttataat gccttatcgt
     4981 taagtatata ttcgtcactc cctcggccga ttcctgcctg gcaaaaaaat tatttagaaa
     5041 aaaacgcttt atttttaaaa gctcagctcg aatccagcct aaatgacagt gtaaaatgtg
     5101 aaaatagagt ataaactttc attttcttat gatataatgt taattatagc atacataaat
     5161 ttactttttt gtaacgatta agaaaggtgg tttttatgac acacgaactc gtaagcgcga
     5221 ccttacccgt atggagcatc attccttttg ccgggatgct cctatccatc gccatcgttc
     5281 ccctcgttcg accggaatgg tgggaaaaac acatgcttgg cgtagctttt gcctggtctt
     5341 tattattcct tattcccttt gcagttgctt atggcatcgg tgagtcatgg ttccgccttc
     5401 tggaatcggt cctcctcgac tacataccct tcatcgttct tctcctgggt ctgttcgtcg
     5461 ttgccggcgg catcgccgtc cgtgggactt tagccggaac gacgaaggtc aactgtcttc
     5521 tcttattcat cggcacggct ctcgccagct ggatcggcac gaccggtgct gccatgctgc
     5581 tcatccgtcc tatgatccgt gctaacgctt ggcgcaagaa taaagtccac ctcatggtct
     5641 tctttatctt cctggtagcc aatatgggcg gctgcctgac ccccctcggc gatcccccgc
     5701 tcttcatggg cttccagcgc ggcgttccct ttacgtggac cttccatctc ctgccgattc
     5761 ttctcttgaa cctggtcatc atgtttgcca tctttgccct gatggataag catttttata
     5821 ataaggaaat aaaagaaggc cgctcgcctc atgacgccat tgctgccggc agcaaagaac
     5881 cgatccgtct tgaaggggct cataacctga tttttatcgc tatgatcatc atcggcgtat
     5941 tagccaacgg cctcctgccg gaaatgcatc cgttctttgc caacggtgcc ggcatccata
     6001 tttacgacga aatcgtcttc ccctatgcaa ccttagctga aatcatcatc atcttagtcg
     6061 ctgcattcct ctcgattaag acgacaaaaa aatcgacgcg agacctcaac gaatttacgt
     6121 atgctcctat tatcgaagtc gcaaaactct ttatcggcat ctttgtcact atgattcctg
     6181 ccttgattct gctcaaagcc cacggggctg aactgggaat gaacaatccc tggcagatgt
     6241 tctgggctac cggcgccttg tcgtccttcc tggacaatac gccgacgtat ttggtattcc
     6301 tgcaaactgc cggggcgctt ggcgccacgg aaggcatcac gacctccgtc ggtacggttt
     6361 ctcaaatcat gttggaagcc atctcagccg gtgccgtttt catgggt
//
LOCUS       DAWDXZ010000035         6395 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3656_length_6395_cov_8.545426, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000035 DAWDXZ010000000
VERSION     DAWDXZ010000035.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3656_length_6395_cov_8.545426"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    244..253
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    537..546
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1525 a         1783 c         1509 g         1558 t
ORIGIN      
        1 atggaagcca gttcatcttt tgccatcttt tccaaggtct ctgaagagcc gtccggaaac
       61 agcaaggtgg caatattggt cgtcccctgg aagccggcac cttcggcgct gacatcgttg
      121 gctacgagca tatcgagatt ctttttctga agtttcttgg taccgtattc gatgacattc
      181 gtcgtctcag cggcaaaacc gaccaggacc tgatgggttt tacgctggcc aagaccatag
      241 aggnnnnnnn nnnaatcggc aacggccgcg gctttaatga ccaagtcgca gtcgtcataa
      301 tacgattcga cagccgcttt catgtccctg gtactgccga cgtcgatgcg ccgtgcaagg
      361 ccgacaggag ttttcaagtt gtccgtcgta ccggcaacca gagtaacttc ggccccggca
      421 gcaaccgccg ctttggcaat agcaaagccc atgcggccgc tggaatgatt gccgatgaag
      481 cggaccgggt cgatgggttc ccgcgtgccg ccggccgtaa caatgacttt cttcccnnnn
      541 nnnnnnccct tgagcaggtc atttttacag gccagcaatt cgatgtaatc gacgatgacg
      601 tccgggtcgg gcaggcggcc gatgccggtg atgccgcagg ccaggtatcc gctgtccggt
      661 tcgatgagat gatagccgaa ttttttcagc ttagccatat tatcctgggt aatggggttc
      721 aaatacatat tgctgttcat agccggagcc atcagaaccg gcgctgtcgt tgccatgact
      781 gtcgtcgtca gcatatcgtc ggcaatgcca ttggcgatct taccgatgat attggccgtc
      841 gccggtgcaa tgaccaggac gtcggcccag cgggccaggg caatgtgttc gacgttgaat
      901 tcaggaacat cttcaaacat ggaaacggca acgggatttc ctgagatttc ccgcagcgtc
      961 agcggcgtaa tcaactccgt tgcatgggcc gtcatgatac acttgacttc agctcctgac
     1021 ttccgaagtt gactggcaac ggatgcggcc ttaaaggctg caataccgcc cgtaatgcca
     1081 aggacaatct ttttcccttc caaactcatg gcaagtacct ccgtgtcatt taatgccgtc
     1141 gcgaggggct tcataagtaa tcttattctg gtcgatttct tccatggcga tggaaacgac
     1201 tttgttcgta tcgacattga ccaaggcttc atcgtggtcg gtcaattcac gagcccgttt
     1261 ggcagccaag gtaaccagtg tatatttgct gtctactttc tgcatcaaag attcaagggt
     1321 cggtttaatt aacatagttc atctctccta atcatttata caattctttc gtatgggcaa
     1381 tgtttttagc attgcgcatg actttacact gttcggcttt tatgatcgat gccgcttcat
     1441 cgatggcggt ttccaaatcg tcattgacga tgacatagtc atagcggtcg atccagtcga
     1501 gctcttcgcg agctttggac aagcggccgt caataacctc ctcgctatcg gtctggcggc
     1561 tgcgcagtcg gtgttccaag acagaacggc tgggcggcac gatgtaaata aagatgccct
     1621 tgggatagcg ttccttgacc tgcatggccc cttggatatc gatttccagc atgacgtgct
     1681 gcccttcgtc cagcatttgg tagacgtatt ttttcggcgt accgtaataa tgatcgtaga
     1741 ccttagcgtg ctccaaaaaa gcatcttctg agagcagttg ttcaaaacgg gcattgtctg
     1801 agaaataata attgactccg tcgacttctc ccggtcgagg ctgacgggtt gtcatggaaa
     1861 tagaatattg gatattggga aactgtttgc gcagggccgc gcaaatcgtc cccttcccgg
     1921 ctccggacgg gccggataca acgatgagca gtccggaatc attcatagta ttactccctt
     1981 tcttcttccg ctgcatcgat ggcatcagca tcactgcctg ccgatacgat acggtgggaa
     2041 atggtttccg gctgaagagc cgacaagatg atccagccgt tatccatgac gatgacggac
     2101 cgcgtcttgc ggccaaaggt cgcatcgatg aggttgtttc cgtctcgggc atcttggata
     2161 aggcgcttga ttggagccga ctccgggctg atcatggcta cgaccttatc ccccatgacg
     2221 acattgccga agccgatatt taataaattg aagttcatca ttcgatattc tgcacctgtt
     2281 cgcggatttt ttcaatttca tttttaagct gcagcaccga ctccgtgacg gctaaatcac
     2341 cggccttgga gccgatggta ttggcttcgc ggttcatttc ctgaatgagg aaatcgagtt
     2401 ttcgaccgac ttcaccgtca ctttgaagca tggtgtgaag ctgattcaaa tggctttcaa
     2461 aacggacgac ttcttctgtc acgtcgacct tatcagaata catggccact tcctgcaaga
     2521 gtcggtcttc gtcgggcacg ggcgcctgcg ccttatcgat gagctggcga attttctttt
     2581 ccagccgctc ttgataagcc gccgtcatgg catccttacg accggcaatt tcttcgacga
     2641 ggcctttcat gacagccgac cgggacagca aatcccgacc gatgttggcc ccttcgcgaa
     2701 ggcgcatggc aaccatgctg tctaaggcgc cctgcaaagc cgcttcaaag ggcggccagc
     2761 tggcatcggc atcgatagcc ggcggcgttt ggacgaacca atctttggtc agggccatga
     2821 cctcacccaa gccgacggga ctaccgtcaa aaaagcgatt cttggctcct tcaagggccg
     2881 tcttaacggc gcccagtgca gcatcgtcaa tatggatttc cggcgctgcc ggcgagaggt
     2941 cttgaacggt cacaaagaca tcgaccttgc cccgcttcaa gacggcttta atgccgctgc
     3001 gcaggcggtc ttccaaagcc aggtacgcat ggggcatccg tatattcaat tccaaaaaac
     3061 gttggttcac tgcccgcatt tcaatggtaa cagccagttt gccgtcattg ccggttcccc
     3121 tgccaaaacc agtcatactt cgcatgttag aaccccctta ataacctatt caatacctca
     3181 ttatagccta tccggcgtcc atattgcaaa ttttttatat cgatgccacc gtatgcccgt
     3241 ctttcatcgc ccttacagaa agctgctaca cagccgattt tccggcacag ccccttgcct
     3301 ttctccgccc gtttgctaca ataaagtaaa gaaaagagtg aattcgacct atcccagatt
     3361 ttcgttaaga accattctat ctgacggagc tgatttctgc ctttcatctc cctgccttgc
     3421 gtcaggaggt ctgccttatg agcaaacatt acccgaattt ttcccccttg ctggactgca
     3481 tagccgcttt cttcggaatg atatacgcca tttgcctttt aattttggtc cttctcctgg
     3541 ccgtccccat cgtaggtgtc atcatctatc tttgttcttc cctgctgttc ccgacagact
     3601 gggtgcttcc catcacagcg gccggcacga tactctgtct attcataaca gcgatttcgg
     3661 catggtgtga atggtgattt tccctcatcc gccccttctt ttctgaaggg gattttttat
     3721 atcccttcac aggaccttta caaacatggt atgataaaaa ggattgaatt ttttagtgta
     3781 ccagataagg aggtacttct atgaacttag acggtatcac attacactgc attaccaacg
     3841 aactggcgga cattctccaa ggcggccaga tatctaaaat ctatcagctc gacgcccggt
     3901 ccctgtactt ccgcatcttt aatgatacag gccttcacca tttggtcatt actctcgatg
     3961 attctccgcg cctttacatt gccgacacgg tcccgccgac gccggatatc ccgacggggc
     4021 tctgcatgtt tctccgcaag tattatgaaa acggacgcat cgcttccatt gcccaactcc
     4081 atttagaccg acttatcgaa atcgccgtcg atgtcctcga tatctccggc aaggtcgtca
     4141 cccgtaagat ccatgtcgaa ctcatgggaa aatacagcaa cgtcattttc acggaagacg
     4201 gagtcatcat cgaagccctc attaagaccg gtaaaaacaa acaggccgtc cgcaccattg
     4261 ctcccaagga gccctatgat tttccgccga atttcatgcg catggatccc ttttccttct
     4321 ctgcccaaga actgacggat atgatggaaa ccggcggcga cgaaccctta aagacctgga
     4381 tgctcaagcg cttcaacggc atgagcaccg tcgtcttgac cgaattatcc cacgactccg
     4441 gcatcgacac ggccaccctc gtacaggatc tgtccccggc tgaccgcttt gcttggtgcc
     4501 gtacggtcga agcctttggc aagcgcctcg ccgggatttc cggcgcctac gtctataccg
     4561 tcaaaggcaa ggaagtcatc ttccccttgg aactaaacag cctctcccag tatccgcggc
     4621 gtcacttccc cctcatcgaa acgtatctca gcgaatacca ggccacgcac cgcagcctga
     4681 acggcgaaca ggaacagctg caaaagaagg tcgccaagct cattgaaaag caaaaacgaa
     4741 aaatcaagcg cattgccgat gaaatgaaag aaacgaagaa aatcgacacc tataaattgt
     4801 acggcgactt gctcatgatt tacgcttatg aaaagcacga ccatgaaaaa caagtaaccg
     4861 tcaaaaacct cttatccgaa aaccaggaag acgtcaccat cgccctcgac ccggcctact
     4921 ccatgaccga caatgccaac cgctattaca agcgctatac gaagctgcgc aaccgcaaac
     4981 agatatcggc tcagctccac gaggaaaacc aacagcattt agactatctc tactccctcg
     5041 aatatgcctt ggaaaacacg acgacgaaag atgaaatcac ggacatcaag gccgaaatga
     5101 aacagatgaa cctcatcagc ggccataata aagataggca aaagaaagaa tcggctcagg
     5161 acatcttaac catcgacgcc gacggcctga ccatctgggt cggccgcaac aatcggcaaa
     5221 atgacttctt gaccttgaag aaagctcatc cctacgacct gtggttccac gtcaaggacc
     5281 aacccggttc ccacgtcatc ttagcctcgc acaacgccga tccgacggat gcccaaatcg
     5341 aacgggcagc acaactggcc gcctattaca gtaaaggccg ggacagctct aaagtcgaag
     5401 tcgactgcac cctcgtacgc catgtaaaga aaccggccca cgccgtcccg ggctacgtca
     5461 tattcaccaa tcagacgaca tacatcgtcg agcccaagaa agaatagccg catcgaaatc
     5521 aggacgacaa aaagctctcg caactgcgag agcttttctt atttctgttt caaataccag
     5581 ctcaaaggct tggcctttgt cgtcgtatgc cccttatctc cgccggccaa cgaaaagatc
     5641 agggaatcac gatacatctt cgctaccgct tccagcgtat ccaggcggta gaaagcttct
     5701 ttaagatcgg ttcccaaggt aacggcaccg tgccgttcca tgaggaatac tgaggctgtt
     5761 tcagcggcag caccgcatcc ttcagcaagt tccaacgatc cgggaggcgc atagggcaca
     5821 gtcggcactt cgcctaagac catggcccct tcggtcacga tgtcggctgc aaaaggaata
     5881 ccggctacgg tcagggccgt cgccgtaacg ggatgagcat ggatgatggc atgaacatct
     5941 tgctgcttcc gatagatgtt gagatggagg gccgtttccg atgacggttt ccccatcccc
     6001 tcgaaaacga tgccgtccag attgacgacc aggaggtcct tcggatggag ctcgcccttt
     6061 ggaaggcccg acggggtaat gacgataagg ccgtcgtcac ggcgataact gatattgccg
     6121 tcacaggcag ccaccaatcc gcccttcttc atggcctttc cgatggcaca gagtgttttc
     6181 ttattatcca acattcgtcc tccttagcgc atagacggca tattgcggcc gttgtagatt
     6241 tccagcattt cctgccggac ttggcggcgg atttgctgct gttcccgttc actcaaatcg
     6301 tctttggtaa tattgaacag gtagttattt aaatcgaagt cgtggaggac catcttcgta
     6361 tgaaaaatat tctcctgata gacgttgaca tcgat
//
LOCUS       DAWDXZ010000036         6346 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3684_length_6346_cov_7.948498, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000036 DAWDXZ010000000
VERSION     DAWDXZ010000036.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6346)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6346)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6346
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3684_length_6346_cov_7.948498"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    724..733
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1292..1301
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3092..3101
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1519 a         1688 c         1570 g         1539 t
ORIGIN      
        1 ggatcatgtt ggtcgaaccg gctttcgcaa cagggacacc ggccgtcaag acgacgagat
       61 caccggcttt gatgaggtcg ttatcgaggg ctgcaaagat agcattgtcg acgacagcat
      121 cagagtcaac ggcttctttc ccgttaatgg cctgaacgcc ccagttgagc tgtaagcggc
      181 gaacgatggc ttcgtgcgga gatacggcaa cgatggggca gtccggacgg aatttagaaa
      241 tcatctgggc tgtgccgccg cgttcggtcg aagcgatgat agctgcagca tggaggtctt
      301 tggcaattca aaaggtctcg acgtaaagtt gctttccgta aaggtagcga cgcgagccat
      361 ggtttcaacg gcttctaccg gatagagacc gccggccgtt tcgccgctga gcatgatggc
      421 atcggtgccg tcgatgatgg cattggcaac gtcactcgtt tcagcacgag tcggacgagg
      481 gttgttgcac atcgattcca acatctgagt tgccgtgatg accggttcac cggcttcatg
      541 gcatttgcgg atgaggactt tctggagcat cgggacttct tctgccggta cttcgacacc
      601 aaggtcacca cgggcgacca tgatgccgtc gctcatctta atgatttcat caatattatt
      661 gacagcagcc tgacattcga tcttagaaat gatcttcatt tcgctgttgt gcttttcaag
      721 cagnnnnnnn nnncggatgg taaggacgtc tttgccgcgc tgaatgaagg aagccgcaat
      781 gaggtccata ttcatacggc agccaaattc aatatcggca gcatccgttt cagagacagc
      841 cggaaggttg atggcgatac ccggtacggc aacgcgctta cggtcgctca tcttaccgct
      901 gttgaggatt ttcgtatgaa tatcttcgcc ttcgacgctg acgacttcga ggtttaccaa
      961 accgtcggat aagaggatat ggtcgccggc cttaacgtcc tggggcaatt ctttatagtt
     1021 catagaagca atcgtttcgt cgccttctac gtcacgagcg gtcaaggtaa attcattgcc
     1081 ggcttccatc atgatcgagc cgtttttgaa tttgcccaaa cggatttccg ggcctttcgt
     1141 gtcgaggagc agtgctacgg ggattcccaa ttttttggaa gcagctcgaa ccatgtcgat
     1201 gcgctgttta tgttcttcat gagacccgtg ggagaagtta aaacgggcaa cgttcatgcc
     1261 ggctttaatc atcttttcaa taatttccgg annnnnnnnn ntcttggttt tttttagcat
     1321 acagatatct ccttttctac tcacatagac gtaacgattt acgtttctgt tcccagtggt
     1381 acgatttttg tcccactagt gcaatatacg gctattgctc acttgcgatt gtaccgtatt
     1441 cccccaaata ttgcaagata aaaagagggg agactttata aattccccct ctttttgtta
     1501 tctttttgtt agaattacca aaggtgaacc cgcttcgcgg gtgccacgta cattccgtct
     1561 cccgatgtga ttccataggc tttataaaag ccgtccgtcg ccgataaggc accgttgaca
     1621 cgagcttcac caattggatg gggatcgttc tggatcaagt acagcagcat ttgatcggtc
     1681 atcttagttg caaaaatagc cgcaaaggaa tggtagatat cgcgcagaac ggtttcatcg
     1741 cccttggcaa gctccgttgc aaccgacagg ccgccgcagt cggcaatagc ttcattactg
     1801 accaacttcc cattttcatg gagacccttg ccgacggtat agttatcgta ataaggacca
     1861 aaagcagcag acagtttctg aaaggcctcg gcatcctttt tcgtccacca gttttttaat
     1921 cgtccttcac tgtcatactt actgccattg ggatcaaagg catgagagat ttcatggccg
     1981 atgacgacgc cgatgccgcc gagattggca ccgcgcgaag ccttagcatc atagaacgga
     2041 gcctggagga taccggccgg aatggtgatg ctgttgtttt cgggaatata ggcggcgttg
     2101 acgtcctgag gggcaaaagc ataccacttg tcgggattaa aattcgtccc cagaagagca
     2161 tggacctgct gccgttctaa gacgctgtta tgcatgatat tggtcagtaa atcgccgccg
     2221 tcttgaggcg caatgacgtc gggcatgctt tcaatgatcg gtttgtcatc gtctgccggc
     2281 ccccctacaa agacgcgcag actgttcagt ttttcaatgg ccttggccct cgttttggga
     2341 ctgagccaat cgttggcctc aaggcgcagt ttgtagacgt ccctaatttc ccggatcatg
     2401 tccttgacat cgtccacgac cgccgccgta ctgtgatttt tcatataaat ctgaccgacc
     2461 tcataggaca gcaagaattg gaccatacgg ctggcgcctt ccttgtcggt atgagctttg
     2521 gcaatgccga aacgctggcg caaatagtca cgctgaaggt cgcgcagttt gatatgagcc
     2581 ataggggcaa agccgttata tacctggtaa acggcataac tctttaagag gtctaaatta
     2641 tcggccgtat agagcgcatc gaagtgctgt aaataatcgg gatttgacag gaaaaactgt
     2701 ttttcttttg tcaaatcaag gccggctaat atcgtttgag cccccatatg gggcatgagt
     2761 ttttttagct caccttgcga tacgaggcga ttttggacgg tcacgtcatt tcgctgttca
     2821 ctggtcaaga gatggtgccc cagtttttgt tccatggcaa aaatagcttc gctgtgggct
     2881 gccgccttat ccgacgtttc tccggcctct tccaaaacgt gggcaacgta atcacgataa
     2941 gccttccagg cgccgggttg aggttctttt tccaattcat ctcgcgtaaa agacggctcg
     3001 gtcacaagga ttcggggaat atagcgcaga ccggttggaa tgcggtctgg catatagtcg
     3061 ataaagacac cggcccctgt cttttcactc annnnnnnnn nagggccttg gtcaactcgg
     3121 tcagattcct tgcctctttg atgggttccg tcaaagcctt cagcttcccc ggagccgttt
     3181 catttcgttt ttcattatcc aaggcgctga catagatgtc ggcgattttc tgttccggag
     3241 agcctttcgg ataggttccc tgtttttccg caatggcttc cacctctttc cttaaggcct
     3301 ttttattttc taaggagcgt tccgaaaacc acgaccacga ggcctctgtc ggctcgattt
     3361 tcttttcctg taagacgccg tgattgacag cctgataaaa attatccttc tctgagacat
     3421 ccgcagcata gaccgcaccg aagcccatga gactcatcaa gatgaccgcg gcgagctgcc
     3481 gttttatact gcccttcata agtgcaccat cctctccaag attcaaggct ctcctttgag
     3541 agctgctttt tgccaccacc aaaacttact gcagtgccgg atccgcaacg ccgttggcgg
     3601 caaaggccgc cgcccgatca cgacaggttc cgcaaacacc gcacggttta tcgccgcctt
     3661 cgtagcagct ccaggtcagt tcatagggtg cccccaggcg cagcccttct ttcacgacac
     3721 cggccttatt taaggacaat aacggcgctt ctaaatggca ggttcttccc gatccttcat
     3781 aaatagccgt attcatagcc gtttcaaatt ccggggtaca gtcaggataa gcccgtccgg
     3841 cagcatcgtc ggcatgggct ccgtaataga tcacttcggc gccgacactg acggcaatgg
     3901 ctgcggcaaa agacaaaaac aatccgtttc taaaagggac atacgtatcg accgtccctt
     3961 ctcctccctt ttctgccaat tgatcagcgt aggattccgc ggcgattttc ttgtgacttc
     4021 cttgtaagag cgaactgtcg cttaaagaaa aggcctggga taaatcgact tctcgatgag
     4081 cgacgccgta atgagccgca acgtttctgg ccgattccaa ttccttggcg tgcttctgtc
     4141 cgtaatagga cgtcagcgct aagacgtctt ctttcttata tttttctaag gccatggcca
     4201 gacacgtcgt cgagtcgacg ccgccgctga gcaatacaac acatttcata aaaagcctcc
     4261 taatggataa ttaagtttct cacagctccc cggctgtcac tttggccgtt ttcccttgcc
     4321 gaacgatgca gacgataccg attaaaatca tgaccatgcc gatgagggtc atcatcgtca
     4381 gcggttcatc caggaccaag acgccgctga ttacgccgac gacgggaacg ccgaggatgg
     4441 cgattgccgc cttggccgct tcgatttggg tcaacagata gttccacatg aaaaaggcca
     4501 aggaggaagc catgacgccg ttatagagca gacaacccac ggccagtggc gtccactgga
     4561 tcgtcccgct ctccataacc gccgcctgaa tccccagggc caaggcgccg acgaccatct
     4621 gccacgtcgt gtattgaatg aggttacagt cattagactt catgcggcgg tcctgaattt
     4681 taacgataat cccagctgcc gcccaggccg ccgcaccgat caaagtcagt acgacaaggg
     4741 atacatcgcc gccgctgtcg acattcatga ggagacacat accggccata ctgacgatga
     4801 tgcccatgac ttttcgccgc gtcaagtgct cgcctaagaa gaaatgcgcc atcaaggcca
     4861 tccacacggg catgctgtaa ttgaggacgg cgaccaagcc cgctccgagg gttaccatgc
     4921 cgatttgaat ggccgccgta ttgacggcaa tctgcaaaaa tcccgtaatg gcaatccacg
     4981 gccaatagcg acgcggcggt atcggcagtt tcaaccaaaa acagaccgcc aacagtacca
     5041 aggccccgat aatgaatcgg tagcaggaaa aggtaaccgg cggaaaaaag agattggctt
     5101 ctttcatgac gacccagttg aagccccaaa ccagataaat ggcagataat aacttaatca
     5161 ccgcactccc tcccatgaca agtatatatc tccattataa cggttcgcat ccgatttgtc
     5221 gcggtcaaga tataccatgt atcttataag tcgatgtatg ccgataattt aaatcattat
     5281 atggataatc tagtcgttac cgcagccatg tcgtctcggg tgtcgacatc gaagagttcg
     5341 gccgcatcgc cgggatgaca gacccaggta tgatccgact gaatcagatg acggcccccg
     5401 acatcaccgg aaagagctaa gagttcatct ttgcaggagg ctcggaacaa ggccgggctg
     5461 caccgccgct ccccattgtg catacagccg acagccgtac cggaggcgat aaattgacgc
     5521 aaaaagagag aaatcgtctg ccccgtcaag agtggctggt ctgccgggaa aaagccccaa
     5581 acatcccggt cgggataagt gccgacgccg attcgaatcg aagcggccat gccttctaag
     5641 gcctgagggt tccaaaggac gtcgatatgt tttcttcggc aggcctcggc aatgtcatca
     5701 tgggcggtaa cgacggcgac ggaacagtca ataccggaca atggcaaaaa agcctgtgcc
     5761 ccctgacaca ggctggtaag cccataggaa tataaaggtc ggcccgctgc cacagccgat
     5821 agtttattgc ctccgaaacg acggccaaag cccgacgcca taaggattaa ctgtatcttc
     5881 acggtagatt cctcgcttcc tcttcatagg ccgtcatacg tcccggccga gacagatgct
     5941 gcaagattcg atggccgcac tgaaagagag cttcgtcatc aacctgattc aggatgaccg
     6001 tcgtccggtc ccaaaaggag ccgagacgag ctaaacagcc ttcctccata atgcgggcca
     6061 agagtttttc tgtaatgacc gcatcgtccg aaagtcctaa taattcatag cgaaaacagg
     6121 cctcaccgac agtctgtccc agtgccgaca ggccggccag acagtaaacg gcatcgctgt
     6181 tttcgggaat caccggttca tgaggtccca tgaccttcaa aggcatgcga cgcgatccgt
     6241 cgccttcgac taaaacgagg tctgaaagct cgcaaagagc ctcataaaca ccatcgccgg
     6301 gataggttat tttcccatta tcaagggctg tgccgacgac gataat
//
LOCUS       DAWDXZ010000037         6324 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3697_length_6324_cov_9.142447, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000037 DAWDXZ010000000
VERSION     DAWDXZ010000037.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6324)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6324
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3697_length_6324_cov_9.142447"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5721..5730
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1538 a         1642 c         1618 g         1516 t
ORIGIN      
        1 aatcaaattc gttcatttat gtgctcagct cattcaagtg attttattca catagaaata
       61 aaaaaaagaa ggaaactgtc atataaggga caatttcctt ccatgaactt ccataacccg
      121 atgatgaatc ggtcgggtca atcatttcta tttaagaaag cacttacata ccggtactcc
      181 gagacggctg ggccgtcgtt tcaattccgt gacgggtctg gtatttcaag aatgccatca
      241 gtaccaaagc cactacgaag gctccgacga agaagtacag cgtcgtagcg tagctgttcg
      301 tcgtttcttt gatgatcgat accaagaccg gaccggcaac gccggcagcc ccccaagccg
      361 tcaggatacg gccgtgaatg gcgctgagat acttggtccc aaaaaggtcg ctgaggtaag
      421 ccggcatgca ggaaaaaccg ccgccatagc aagtaatgat gagcaggagg aggatttgga
      481 agctgaaagc gctggttaca ttggcaagct gccagaaggc caggatttca atggcgaaga
      541 ataagacata tgtcgacgaa cggccgatat agtcggaaac ggtagcccaa gcaatgcggc
      601 cggcaccgtt caagagaccg atgaggccga ccatagaagc tgctgccatg ggagacatgc
      661 cgacgacatc ctgggccatg ggagaagcga cggccaagag ggcgatgccg caggtaatgt
      721 tcgtaaagaa gagccaccaa atggcgtaga actgccacgt tttcatggag tcgcgaacgg
      781 taaactgagg ctgtgcatcg gcatattcaa cgttcgattt cttttcagca gccgctgccg
      841 aatcttcttc gggaggcgct aaatataagg aagaggccgt cataatgacc atatatacaa
      901 taccgaggat gatgaaatta gccaccagac caaaagactg aaccagaatt tgcatggcag
      961 gacctgcaat caagctggca aaaccgaagc ccatgatagc caaacccgtt gcaaagccgc
     1021 ggttttcagg gaaccactta accaacgtcg ataccggcgt aatgtagccc gtccccaggc
     1081 cgatgccgcc gatgacgccg tagaacaagt agagcatggg caggctgtgc aggtagaggg
     1141 cagctgccgt accgaacata gccaagacga agaaggtcat cgacaagagc ccggatttgc
     1201 gaggtccgat tttttgaaca aaactgccca agaaaccggc cgacaatccg aggaataaaa
     1261 tagctaaaga aaaagtccac gtcgtttcct gcaggctaaa gcccatagct tccatgatcg
     1321 gcttggtcaa aacgctccaa gcgtagacac tgccgataca aatatggagg ccgatagccg
     1381 acaaagcgat gagccaacga tttcttttca tgatccatta tctccttcca ggtagggagc
     1441 caagaggtct gaaaaaaaat ataccgaccc ccgggcccaa gaatatgtta gtagcataca
     1501 cttgcccaga cggtcggcat gaaatggctt cgtacagttc acgtggttgc tccacttgaa
     1561 tccgtcagtc ctgtacgaat ctgctgatgt tattatacga atattgattt attttgtcaa
     1621 tatcaaattt taagcggccg gagaaaaagg aacgtaatcc ttcgattcct gtgactggcc
     1681 ggcagctttt tgtgtcgttg ctgcactcga cgattgttcc tgcccctgct gagcggctaa
     1741 ttcagcagcc tgctgctgct tttcctgaat atccgataag gtctggcctg ccggtgcatg
     1801 gcgcacgatg tagacatttt ctgccggttc cgcatctttc agagccgaaa aagcgtaggt
     1861 catcagtttc tgggcatcgc catagcggtc actcgaattc atgatcgatg cgatgaccgt
     1921 ccgaccgtct ctcgttgccg aaacgacaag acaaggacca ccggcattgg tcgtaccggt
     1981 cttgatgccg ttggcgccgg aaaagccgct ggtcaaaaac tcgttggtcg acgtgcagtg
     2041 cttaaatccg ccgtccatat agggcatgtc gaattcgctt cggctgacca tatcctgaaa
     2101 ttcaggaatc ttcatgccat aggcagcgat aacagccatg tcgcgagccg tcgagtaatg
     2161 gttcgtatcg ggaaggccgt gaggattggc aaagtgcgta tgagaagcgc ctaaagcagc
     2221 tgctttttca ttcatcttgg cgacaaaatc agattctgtc ggcgtcaccg tttcggcgac
     2281 ggcaacggcc gcatcacagc ccgatacgag catcatgccg gtcatgccat tactgagggt
     2341 aatctgatcg cccggtttga tgccgaggac cgaagcatca ctttcgatgt catagatatc
     2401 attcgtaata ttaaggggtt tatccatcag attttgactc ttcccgagct caatgccgag
     2461 gatggccgtc ataattttcg tcgtactgcc gggataggcc aggtcatcgg catttttcgc
     2521 atagagttca tcccccgtat cggcatccat gacataggct gcatgagcct cgatatccgg
     2581 tttctgcagt tcatcggcaa agaccggcgc tgccatcgtc atacaggcag cagcagccaa
     2641 acaaatcatt tttttcaaag acataattgc acctctcctt tataaaggat tttttacagc
     2701 cattttcggc ttgcgcggat attttttagg actttcgcca gtcttttgaa tatagatgac
     2761 ggcccgcacc tcatcgagtc ccggaagctg aacggccttc acatggtcca ccgtaccgcc
     2821 taaaatcttc aaggcctttt cagcttcttt cagttcgtcc tcatactggg cgccctttaa
     2881 agaaatgaaa taaccgtcct gtttgacata gggcagagcc cattcggcca gaatatttaa
     2941 acgagccacg gctcggctgg tcacgacgtc aaaggcactg cggtagatag gctgatgggc
     3001 cgcctcttcg gcccgaccgt gaaggaaatc ggcagtaagt cccaagttct gcaaaaaagt
     3061 aagtctcttt tgcagggagt caaaaaaggt aaccttcaaa tccgggcgat agatgagcag
     3121 gggcaggccg ggaaaaccgg cgccggttcc caaatcaaga acggacgccc ccggcgtaaa
     3181 gcaatccgct tcataacagg aaagactgtc ggccatgtgt tttatcgcta cttctttagg
     3241 ctcggtaatg gccgtcaggt tcatgacctt attggtttca attaattgtt catgaaagac
     3301 gtcgaattgg cggagctgtt cctccgtaac cgaaagtccc atggctgtaa gagccgattg
     3361 taattcactg ccaaacatat cacttatccc cccggttgtg ttgttccaag agaatcagaa
     3421 gggccgtaat atcggccggt gaaacgccgg aaatacggct ggcctgacct aaggacaagg
     3481 ggcggatttc atcgagcttc tgccgcgctt cggcagacaa agtgtcgatg tcggcataga
     3541 cgatgtcttt gggcattttc ttcttttcca gacggttcat cttatcgacc tgttccatct
     3601 gacggctgat gtagccttcg tacttaatca tgatttcgat ttcttcctca acgtcttccg
     3661 ctaagtccgg caagtcaaag acagtctgta gggacgcata ggtcacgtca tcacgacgaa
     3721 gaaggtcata ggctgtgagg gccgtatgga taggcgctgt gccgagtttt tcaagtttcg
     3781 ccaagttatc tttagacggc gtaactgaaa tttccttcaa ggccgaaatc cctgcctcaa
     3841 tagcctgttt cttatttgtg aatgcctgat atcgttcttc tgtaaccagg ccaatatcat
     3901 agcccttctg ggtcaggcgc aaatcggcat tatcctgcct caggatgagg cggtactcac
     3961 tgcggctggt catgatgcgg taaggctcgt tcgttccctt ggtgactaaa tcatcgatga
     4021 ggacgccgat gtaggcttcg gaacgggtca ggacgaaggg atctttatgc tgcactttta
     4081 aagccgcatt gataccggca atcagccctt gagctgccgc ttcttcataa ccgctggtgc
     4141 cgttggcctg gccggctgaa aagaggccgg aaatcttttt cgtttccagc catggcgtga
     4201 gctgcgtcgg atcaaggcag tcgtattcga tggcgtagcc gggacgcatg atttttacgt
     4261 cttcaaggcc cggtatcgtc cgcaggaagg cgtactgaac gtcggcaggc atactggtac
     4321 tcatgccctg gacgtacatt tcttctgtcg attccccttc cggttcaacg aaaagctggt
     4381 gccgcttttt atcggcaaag cggacgattt tcgattcaat ggacgggcag taccggggac
     4441 cgatgccttc gatgacgccg ttgcacatcg gtgcccgatc aaggttgtcg cgaatgattt
     4501 tatgcgtttc ttcattggta aaggtcagcc agcagcaagc cttgttgcgg ttctttcgtt
     4561 ccgacaagaa ggaaaatgca tggccgtctt tatccccttc ctgcttagtc atttcatcag
     4621 tccgaagggt gcggcggtcg acgcgggccg gcgtaccggt cttaaagcgc atgagcttga
     4681 tcccggcttt taagagcgat cccgacaggt cttctgccga acgctggccg atggggccgc
     4741 cggaataggt gcagtcgcca atgatgattt tcccctttaa ataggttcct gtcgccaaga
     4801 tgacgcagga tgccttatag acttcgtcca gttccgtccg cacgccagaa accttaccgt
     4861 cttcgagcag caagtcagta atcataagct gtttgacatc gagattttcc tggttttcca
     4921 gggtcttggt catgtccatc tgatacagct ttttatcgga ctgcgcccgc agcgaataga
     4981 cagcaggacc cttgccggta ttgagcatcc gcatttgcag gcacgtttta tcggtattga
     5041 ttcccatttc tccgcctaag gcatcgattt cccggaccaa atgactcttt ccgggacctc
     5101 ctacggccgg attgcagggc atgagggcaa tattatctaa atttaaagta gccatcaacg
     5161 tcttggcacc taagcgggca ctggcaagag ccgcttcaca gccggcatgg ccggcgccga
     5221 tgacgatgac atcatatgta tcgacggtaa acatagttcc acctctttat tttcccaaac
     5281 agaatcggct gaagatttct tcaacgatat cgtcatgaac ggtatccccc gtgatagagc
     5341 ccaagaggct ccaggcttcc cgcaagtcta cgacggcaaa atcgacaggc atgccgtctt
     5401 ccatcgtctt cagggcacgg ctcaaggcct cccggctttg gcggaccagc tcaatatgac
     5461 gcgtattggt taaaagagca ctgcccgatt gattcgtacg tggcagggcc tgttccaaaa
     5521 gccggtcttt taaaacctcc aggccttcgc cggtactggc cgaaatagac aggatcggat
     5581 ggtcataagc cgtaagatcg ccttcgccga cgacagccgg caaatcgagt ttattgagga
     5641 cgatgagggc atttttatca gccgttgccg ctaagatgct cttatcttcc tcagaaagag
     5701 gccgagaacg gtcgatgaca nnnnnnnnnn gtgatatcgg ccttgtccat ataggattta
     5761 gccttcgtta cgccaatctg ttcgacccgg tcatcggtct cgcgaattcc tgccgtatcg
     5821 accaaacaga cgggaacgcc ctttaaagaa atccattctt cgagaacgtc cctcgtcgtg
     5881 cccggcacat cggtgacgat agcccgatcg ctttgaagca ggcagttgag caggctcgat
     5941 ttgccggcgt tcggtgtgcc ggcaatggct gccatgacgc cgtcacggat cattcgccct
     6001 tcccgcgaag cagtcaacat ctcatcgagg gcttcaattt gagtccgaat agaaccggca
     6061 acatctggga ccgtaatatc ttctaagtct tcttcaggat agtcgatcat gacttccagg
     6121 cgtgtaataa aatcagtcag ttccttgcgc atggcaccga cggccttcga aagccgtcct
     6181 tcaagctgac tgacagcatt ggtaaggccc tgatcgctct tggcctggat gacatccatg
     6241 acggcctcgg cctgagccag gtcgatacgg ccgttcacga aggcccgctg cgtaaattcg
     6301 ccgggattgg ccaggcgggc ccca
//
LOCUS       DAWDXZ010000038         6300 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3711_length_6300_cov_8.869175, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000038 DAWDXZ010000000
VERSION     DAWDXZ010000038.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6300)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6300
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3711_length_6300_cov_8.869175"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2430..2507
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3221..3230
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4754..4763
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1414 a         1742 c         1519 g         1527 t
ORIGIN      
        1 atatcatagc cacatcaaca aaaaacgcca taaacgcgac gacgcaaaat agtacgttcg
       61 aacccctcgt tttcagagag ctgccggccg gtgcaaggca gtacaggagc cgaaccgaac
      121 tcattgccga gcggagacgc tgaacaaagt aggccgacac gggaactccc gttacagagc
      181 atggatatcg ccgatggccg tatccgagag tgcatccgag aggatgaaac agagtggtac
      241 cgcggaagga tataacgcct ttcgtctctt agcaaatacc ttgcagagag acgaaaggcg
      301 ttttcgtttc tctccctgtc catcaaagga ggaaactatt atggccaata tttcatcgat
      361 taaaacggca tccctcagca tgtgcttttc cgtcggtgcc gtcctgttca gttctcacgc
      421 cggcggcggt tttgccacag gcaatcagga aaacgtctac ttcgtcagcc tgggctggct
      481 cggccccatt acggcgatca tcaccatgct gctctttacc ctgaccatca aagaagccat
      541 gaacatgtac aattcgcggc acctcaccag ttaccgccag ttattccaga acttataccg
      601 cccctttacg ggcatcgaat acttctttga agccttcttc tatatcatgg tcttaatggc
      661 cgtatcggct acgatttccg gtgccgcttc cgccatcaat gcccaaaccg gcctcaacta
      721 ctacgccggt atcggcgtcg tcggcatcat catcttcttc ttgacgatct tcggtgccgg
      781 cctggtccgc cgagcaacga cgtacatggg catggccatc ctggccatgg tcgccatcat
      841 tttctcggtc ggcatctatg cggcaggttc cgttgaagga acccaggcct ttacagccgt
      901 catggcccag gacttttccg ccaacggttt cagcaacgtc ccgacagcca ttctccacgc
      961 cttcacctat gccggtttcc agtgcgtcgt catcccgacc atgatcgtcg tcggtacgcc
     1021 cttagtcacc cgtaagaact gcgccatgtc gatgtggatt tccttcgcca tgaacgccgc
     1081 tgccctgacc ttagccgtcg tcatgctctt aggctggtcc ggcttctacg gtaaatcgac
     1141 actgccgacg ctgaccagca gccaggccat gggattaccg tggctgacca tcgtctacag
     1201 tgccttcctg atgctgtgcc tcatttctac cggcgtcacg acggtcttcg gcttcaccgc
     1261 tcgtttttcg gaaatcaata tcctgaaaaa aatcagctcc cgctcggtca tccgcgctgc
     1321 catggtcacg gctttcatca tcatcgcctc gatgaccgtt tccattgccg gcctgaccaa
     1381 tatcatcaaa tacggttacg gctactgcgg atacgctgcc atcgccatca tcatcgtccc
     1441 cttcctgacc attggccgct ataaaaataa acgctatgcc agtgataagg aataccgggc
     1501 tcgcatcgac agcatgaacg aaaatccgca aacgtcacag gctttcaagc cgactgccga
     1561 agctgccggc cattaattga gataactcaa ggtatcatca aaagacacgt ccgctcacag
     1621 acagacaaaa tgccttgcat ctacgagggt gcaaggcatt tttatcgctt atgatatttt
     1681 ttattcgact aagcggtcag gcactttcag tttcatgaaa ccgtatgtgc cattggcaat
     1741 catcagcaag tcccggtcct gtacgttgag tttctgacgg atttcagcat cccgattcgc
     1801 caaaatcttg gcactccaat tgggatcgat gagggacgag catccggcag cgacgatttc
     1861 cgaatgggcc agtgccgctt ctacgtcggc acgagtgcgg acgtcaccga cggaaatgag
     1921 gggcatgcgg ccgccaatgg cgtcgtggac ataggccagc atcgattttt cctggtattc
     1981 cggaaggttg gtcttgcggt cataacgggt cagggaaata tggagataat ccaatttctt
     2041 atcggccagt ttatcgacga gccacaaagt ttccttcaaa ttgaagcccg gatccgtttc
     2101 ttcccgcggc gaaaaacggt agccgatgat gaagttggga acgttcatgg catcgatggt
     2161 ttcaatgatg ccatcgacaa cgcgttcaat gaaggtatag cgcttttcca ggcttccacc
     2221 ccatttgtcg gtgcggcggt tggaatgggg tgaaaagaac tggtgcagca agtagtggtt
     2281 ggcaccgtgg atttctacgc cgtcaaaccc ggctttgaca gctcgaacgg cagccttttt
     2341 aaagttttcg atgatttctt cgatttcgtc atcccgcagt tcccgcggcg tttcggcacc
     2401 tttcttggca gcagcgacgg cgctgggggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncta caggctgttc
     2521 gccttcgatg gtcttgctgt cggtcatgcg gccggcatgg aagatctgaa ggatagcccg
     2581 ggtcccgtcc cgttttatcg tcgaagccaa tttgctgaga ttgggaatga atgcatcgtc
     2641 atggacgctc aattccccgg tccacccctt gccgttcggc tggatatagg ctacgcccgt
     2701 aacaaacatg ccgacagcac cggtgcgcat gccgtaatag gcgatttcgt cacctgtgac
     2761 cgtgccgtca taataactca tgcgcgtcgt catgggcgga atagccaagc ggttctttac
     2821 cgtaaggcct cgtttgaagg taagggtatc cgttaagtta gtcactgtct tcacctctct
     2881 tgaaagatta tgtcagtata cgtttaatag aggcacgcca tcatgccggc atttcgtgct
     2941 cagtcccggt cggacgcgct taaattcgac gattcccatc ggaattgaat gtagccgtaa
     3001 taacagtgcc tgcatcgtac catctagcgt ggtttgtttt atcatacccc aggccttcca
     3061 acccgtcaag agacgtcatc aaaagaagaa ggagctgccc cttgaggatc tttttctcag
     3121 gcacagctcc agtttcttat aattcaaata cgtctctgcg gcgcaggggc aagtattgtt
     3181 tttcatcccg cagttggcgg gccttttcgt aatccaaatc nnnnnnnnnn agttcttcac
     3241 cggctcctgc caaggccgcg acagatccgt ccggtccggc aacgatggtc tgaccacaga
     3301 aatccatgac gccttcttga ccggtacggt tgaccatgac ggcatagaaa ctgttctgga
     3361 aagcagggat ccgaatttcc cattggaata catccagcgg ttctgtcgtt tcattggcca
     3421 cagccgtaat gaccagatcc gcccctttca gcgccagcgt ccggaagctt tccggataat
     3481 gacggtcaaa gcagacgata aggccgatat tgccggctga tgtcggcacg acgacgaaac
     3541 cgtcatcgcc gggcgagaag taatcctgct catagaaatg gtgggcccgg acaatatggt
     3601 tcttgcggac aatggctttg atttccccat tttcatcgat gaccatcatc gtcggataaa
     3661 tctttccgtc ccaagtcaat accagtgaaa agacgccgat gatgccttct tcccggcagg
     3721 cacggcacag tccttcgata tgcggatcat ccaacggaag ggcgatattg tccttagaaa
     3781 gaccggcata ctgcggtata tattccatca gctgcccttc cggaaagcag accagctgtg
     3841 cgccggattc tttcgcctta tgaagatagg tcagggcctt ctgataattt tcttctaaat
     3901 tttttcccaa ccggagctgg gcacaagcaa tgcgtaccat gagttacctt cctctccttc
     3961 tttattgcaa gatacgctgc aggcggcgaa ccgtttcctg caggacttcc atagaagccg
     4021 caaaaggcat gcggatacaa ttttcagcac ctcggccaaa ggcatccccc ggcgctgcag
     4081 caacccggcc ctcggtaagg aggtattcac ttaaggccag ggaatccatg ccgcggtaat
     4141 caatccgtgg taagacatag aaggcacctt gaggagcccg gcaggtgacg caggggattt
     4201 cattcagggc ctgtacaaaa tatcgacacc gttcatcata ttcccggcac atgcggctga
     4261 cgtcggcttc acaatcgagg gccgtaagag ctgcactctg cacgaaggat gtcgcacagt
     4321 tgagaatatg ctgctggact ttcgccattt gccgaagcag atccggatcg gcagcgacgt
     4381 agcccagacg ccagccggcc atggcatagg cttttgaaaa accgttgagc gtaatgaccc
     4441 ggcctttcaa ttcgggcagg ctggccggac tgatatgctt gaacccggaa aaaatgattc
     4501 gttcatagat ttcgtcggaa aagatgagca aatcccgctc acaagccact tcggcaatgg
     4561 ccttcagttc atcagcctgg cagacgcggc ccgtcggatt attgggcgag cataaaataa
     4621 tggccttcgt ctgcggcgta atgaatcgct caatctcact cttgcgaatc tgatagttat
     4681 cctcataaga caaagggatg ccgatagctt tggcccccgt caattcaata atgggcttat
     4741 aggatacatc ggcnnnnnnn nnngttcaaa atacagggct tcatcaccgg cctggaggca
     4801 ggtaaaaagg ccgatgaaaa gcgctaattt cgtactggcc gtcactaaaa tcccttccgg
     4861 aacggcatcg atgtgatttt cgacctgcag tttctgtgcc agtttccggc gcagttccgg
     4921 caggccctga ctctggatat aagaggtgaa tccggccttc atatctgtat aggcccgttc
     4981 gataatcggt gacggcgtct taaaatccgg ttcaccaccg gccaggttaa tgatatcctg
     5041 gccttcttct ttcatctgtt ttgccaaact catgagccgc accgttgcag acggctgcag
     5101 ctcctttacc cgttggctga taaaatcacg catgactgat ccctcctgtt tcaattactg
     5161 ccacttcttg gcaaagccca tcaaatcggc cgtcgtttgg ccttgctgca acagaacttt
     5221 caattcgtct tctttggctt tcttagcttg tgatttttca agaacttctg ctgctttttc
     5281 cttgggaatg acgacgacac cgtcggcatc gccgatgacg atgtcaccgg gatgaacgat
     5341 tcgtcctccg cattcaatga cagcattgat ggtaccgcat gtttctttca gcgtcccgtt
     5401 cgggcagacg cctcgggcaa agacgggata gttcatgtcg atgatatccc gcttatcccg
     5461 gcaggccccg tcgatgacga cgccggcgat tcccttggcc cggcaggatg tcgcaaagag
     5521 ttccccaaag acaccggcct ggagatatcc cccgcactga atgaccaaga cgacgcccgg
     5581 cttggcttcg gcaatagccc ggtgaatcgt caggttgtcg ccgggcacgc aggtcacggt
     5641 catggcataa ccggtcattt caaaatgata gtccaacggt tttatagcct catccatact
     5701 cccttcccgg ccgttcatat cacaaatgtt ccctgtcggg atacctttaa aagcactggc
     5761 aatatccata gtgattcccc tctcttactg ttcttcgtgt gtctgcaaat gatgatagcc
     5821 catctgtgcc cgagcttctt tcaaggtctt accgctcttg accagggcta caatcttttg
     5881 ttccgtctct tcaatggatc gggcgatttt cagcgtcgtt tctacggcct gcaagggaat
     5941 gcagacggca ccgttttcat cggctacgat caaatctccg gggcaaacct tgaccgacga
     6001 aatagaaaca ggttcgttaa tcgaagaaac ttcaacgcgg tccttgccgg tcatcatgta
     6061 attgcctttt gtaaagatcg gatattttaa cgcttgaatc gtcttaacgt cccggcatac
     6121 gccgtcgatg accgtaccgg ccacgccttt ccattcagcg acctgggaca tgatatcccc
     6181 ccagacagtg cagtactgtc tgccttcatt ggcaatgacg acgacatggc ccgtcggcac
     6241 atcgtcgagg aaatcgccta cagtcccctt agtttgaccg cagggaatat actgtacggt
//
LOCUS       DAWDXZ010000039         6224 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3751_length_6224_cov_8.558113, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000039 DAWDXZ010000000
VERSION     DAWDXZ010000039.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6224)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3751_length_6224_cov_8.558113"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1736..1745
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4302..4348
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5334..5343
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1507 a         1719 c         1544 g         1387 t
ORIGIN      
        1 gcgcgaaata cgttggcatc gggcccgacg tcaaagacca ggctcatcga tttggccggc
       61 gacaggacgg agtctcgatt cacggtgacg ccgacggact gacgggcctt cgtttctcgc
      121 acagcgtcgg cctgggccgt tatgggaatg tcactgcccg gttcgtgacg gcccgtaatg
      181 ccgcagccct tttgccggca gatgagttga agcttctgaa ggacgtctgc ttcaagggcc
      241 atgaggcaag tatcggccaa ggcattgaac aagagagaat ccatgacgtc gccgtctttt
      301 tcatatcggt caagctggcg gctgacggcc tcgcctaacg tcaggatcgc atagaccttg
      361 tcgtcggtaa acgccaaaac ggccttagcc tgcatcaacg ggcgcaggac cggtaccaat
      421 tcctgacagc gagcaaggct cttgtcccgg ccaagaccgg ccttctgata gccggcaaag
      481 tgcagcgcct gatcgacgtc gggaacaatc cgttgtcccc ttaaaatacg aatatcagcc
      541 atgacctttt cccccttaca taattattta agctatatta taacgaaaat cgaaggagac
      601 tgccaccgat aattgcaaat ttatgggatt ttgccgctga caggctaaaa atcgtcccac
      661 ttccacgagt ttaggcacgg cttcctcgtc agttcccctc atagaccggc gtaccgtcgg
      721 cgaccatgcc atctcccttc ttgacggccc tttccgattg gcgtataata acttggtaag
      781 actttacgcg ctgattacat gggcctatag ctcagcggta gagccaccgg ctcataaccg
      841 gttggtcctt ggttcgatcc caagtgggcc caccaattac aacgctttct tcagcaaagg
      901 agaacccgtt acagcacgac acaaaaatac gacgacctta tcccagagat aacaggtcgt
      961 cgtatttttt tgctttttat gccgttaaaa agggcctggc cgcggcacct gaggcgggat
     1021 tccaagaggc tgccgtcagg tatccttgag acttcccgct tcttataaat caataatatg
     1081 tgtagttcct gttgtccgac gacgcggtcc ataaccgatt ctgacgcatc cctatgccgc
     1141 aggcgtccat aatttcaaag atgccttaaa ggacgcgcgg aaacgacttt accgggtgac
     1201 tgtcaccgcc aggttttagc agcatccctc acccttttcg tctcccttcc ttgcagcgca
     1261 tcgagcatcc ggaacagttg ccgccgcatg aatttccgcc gccttttaga acgctgcgga
     1321 cggcaaggac gaataacacg ccgatgacgg ccaataacag aaaactcgcc gcattcatgc
     1381 gtatctcacc tcactttcgc tagacgaacc ccatgaggag tccgatttca cggacaataa
     1441 aagcgacaac ccaggcaatg gcacactgta ccacgacggt aacggcggct gcccgggccg
     1501 accccatctc ccgacgcacc gttgccaccg ctgccacgca gggcgtataa agcagggtaa
     1561 acaccaggaa tataagggcc gtaaagggcg taaacaacgt cgacagggcc gatacatcat
     1621 cacctaataa tacggacagc gtcgaaacga cactctcttt ggccgtaaag cccgttatca
     1681 gggccgtcgc cacccgccaa tcgccaaagc ccaggggctt gaaaatcggt gccgtnnnnn
     1741 nnnnnttctc ccaaatgagg cggacgacac tcttggccga cggcagccga tagttcggca
     1801 gttccatgac gaagggcacg ggttcgcctc tatacacccg ccctttcagc agcagcgcat
     1861 agaggatacc gcagaaaatt ccgaagaaat ataagccgat catgaccaca gcctgccact
     1921 gtttcgggaa aaaggcactg accatgagcc cgtaaatcgg cagtttggca ctgcagctca
     1981 taaagggcgt gaggaggatg gtcattttgc gatcccgttc actggacagg gtcctcgtgg
     2041 ccataatggc cgggacagaa cagccaaagc cgatcagcat gggcacaaaa ctccgaccgg
     2101 acaagccgac gcgccgcagc agtctatcca tgacaaaggc gacgcgggcc atatagccgc
     2161 tgtcctcgag gatagagagg aagaaaaaca aggtgacgat ggtgggaaga aaactcaaga
     2221 cgctgccgac gccattacag atcccatcga tgaccagggc atggaccagg ggattgatgg
     2281 cggcggccgt cagtcccgcg tcgatcaagc ccgtcacggc atctacgccc atggccatga
     2341 gctccgacag gccggctccg ataaagttga aagtcatgac aaaaatgaag gccataattc
     2401 ccagaaaagc cggaatggcc gtatattttc cggtcaatat ccgatccagg gcgacgctgc
     2461 gtcggtgctc ccggctttca tgaggcttta cgacggtctt cgcgcagaca ttttcaatga
     2521 aagtaaagcg catattggca agggccgctt cccgatcaag ggcgctgtcc ttttccatga
     2581 cgcgtacgat ttgttccaac gccgtttggg cttccggcgg caaggccagc gctttcccga
     2641 tcaaagggtc attctcaatc accttggtcg ccgaaaaacg gaccggaaga tcggctgctt
     2701 tcgcataggg ctcgatgaga tgaaccgtgc tgtgaatggc tcgatgcacg gcgccgacct
     2761 gatctcccgg catgtcgctt tcggcacaga aatcaatccg ggccgggatt tcccgatgcc
     2821 ttgccacatg aatggcatgg tcaacgagct cgtcaatccc ttcatttttt acagccgaaa
     2881 taggcactac cggaatgccc agggtttgct ctaattcgtt gatgcggaca gaaccgccat
     2941 tggcccgtac ctcatccatc atattcagcg ccaggaccat ggggattccc aactccatga
     3001 gctgcatggt caggtacaaa ttgcgctcaa tattggaggc gtcgacaata ttgatgatcc
     3061 cggacggcct ggttttcatc aggaaatccc gggttacaat ttcctcactg gaatagggcg
     3121 acagggaata aatccccggc aaatcggtaa ccgtagcttc cggataatgg cgaatggtcc
     3181 cgtcttttcg atcgacggtg acaccgggaa aattcccgac atgctgcttg gctccggtca
     3241 attgattaaa caaggtcgtc ttgccgcaat tctggttccc ggctaaggca aaggtcaagg
     3301 gctgcccttt ggcaatggcg tccgccttgt catggacatg gtagtccccg tccgttccca
     3361 attcccccac gccgggatgg gcaatgggtc ggtaggattc cgattggctc ttgggctgcg
     3421 ccttttgatg aatatcctct acctctatct ttttcgcttc atcgaggcgc agggtcaatt
     3481 catagccgcg cagttcaatt tggatcggat ctcccagggg cgcgatttta cggagcgtaa
     3541 cctcggtctt cggcgtcagc cccatatcca ataaatgatg ccgaagggca ccgctgccgc
     3601 caatggcacg gatataggca ctctgtttcg gccttaattg atccatggtt atcatgtcgt
     3661 tcatcgtatg tctactcttt tgcaatgtgt tcccggcctt tctcaatcac agggcatatc
     3721 tgcctgtcag ccaacggatt gcagatttgt cggccgatac tgtgtgtttt atcaaagggc
     3781 ttgctttccg tatcttgtct gcaacccctt cccttcaagg attgcaggca agatacctgc
     3841 atcctatatg gataggactc aatggcttta tacatctatt caggcagcca tcaaataatc
     3901 gtttaatcgt tatatctgta gtatgatgct atatgagaat aaagtcaatc cattccctta
     3961 cagtctgcca aaagggcgga aaccatgtcc aaacgagcag cagagggcac cttgagagat
     4021 tccgcatgac accatcaccg agaaacagcc agccgcatcg gggcggcacc tgcgtttgat
     4081 atgaggaaaa ccatggaccc cctgacaaga aaagcagtcc cgaacaaggg ggactgcttt
     4141 tctgctatcg cctgatcaac atcctataat gtacactcgg actgcttcac caaggcgtcg
     4201 gcgcatattt ttttactttg cagccgttat cacaaggcca agacattttt tctcaacgag
     4261 gctcttaatc ggtagccgtc aggcgatggt gcttctcccg gnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa taatacgctg agcccgcagg cgaacgaagt
     4381 tcgtcagccg tgccaaggtc ttttccggca agggacaggt cggatgattt tcaaaggaaa
     4441 agaccgtcag cctcttaaga gcctctatat ggcggggctc cacaaagaat tgggccagga
     4501 tatcgaaggg catcgacgcg ccttgcctgc cggacgtccg caggtattca tcgatatgtt
     4561 ccaattcata ggaattggca tcatagagca aagaacaacc attatcaaac aacggtgccg
     4621 gctgtaataa tcggcccgtc cgggtatcgt aaagatagcc gaaatttcca tagtggcggt
     4681 ccttattgca gattaggctg tcaaatatca tcatatcagc atattcgcac cgtccgtaga
     4741 gctcagccat gacgtgctgc atctctaaac tataggtctg aaaagcattc ctatcaagac
     4801 ctttatagga aaaatacgta taggcatcga catagcccac atcggtactg gtgaacagtt
     4861 ttcccaggca taccgtttcc ctcgtcccgt cggaatgagt ataggtttcc agctcataaa
     4921 gagcgtgaga aaatgccatg acggcagcga ccttagcggc gtaccattcc agcacggcct
     4981 gattcaagcc ttcaggagac ggcataaaat cgactccctt tcttaaatag atatcgccgc
     5041 cttttcgaat ccagcatttt ctcagcatcc cgcaagtcgt gatttccggc gtcgcaatca
     5101 tgccggacac agagcctccc gccccggtaa aagcaatctg tgtcagcctg tcatcgaagg
     5161 gattatcgta taaattacag tccgcccagg aagtcgaatc atccgcagcg gtaatccaat
     5221 agacatcatt gagggacaag gctcgcgtca catcaacata accgaggaac cggccgccgt
     5281 caagggaagc gcggatagac tcggaaaaac gacgattccg gggaacttta cggnnnnnnn
     5341 nnngtggaaa agcctttctc ccgtaagagg gccgcctatt ttaagagcca tgggcaagag
     5401 ggcctcctgt tctgaataga acttattgat ttcaatccga tactttgtat acgaggcgtg
     5461 agcgatcatg gctttctcta gatacaggga aaaatcaagc aatgccgtat cgtgagatcg
     5521 cagggtatag ctcttacttc tctcttcttt catcacactc ccctccttaa gtgctcatcg
     5581 acagccgttg actagtaaga gcggccgtac atcgaaaaaa attcagccaa gcgttcttcc
     5641 aagcactcca ttaaaagctc cctcaagggc tggatctcgt cgtccagctc ataggcttcg
     5701 agggcctgaa aatactccgc ctgctgtgct ttttgtatcg aaagaggcgg atagccggcc
     5761 gctactaagg ggtaattcat catcaggcgg gctgtcggtc cgtttcccga caaaaaaggt
     5821 tggatcttac tgaattcacc gtggatccgg gcggcccgct caaggggact agaaaagttg
     5881 tcggcggcaa tataatccgc caatgttttc attctctcac ccacactctc ggccgtcgcc
     5941 aaaacagcct gtgatccgcg gatatagacg gggtactccc tataacgacc gtccatcgta
     6001 tctaaaggcg ctgccaatct acggatgtct cctatcagcg cttctgtcaa gggaaggccc
     6061 gccgctgccg cctctctcag cgctgtccag gcccggtcgt aaccgcgtac ggcctcgtag
     6121 tcagcgactt tggtcccggc cggaatgatg ccgtcgaaga ggatcatctt gacgtcgagg
     6181 acggtcagaa ggctcccttc aatccccgtc gaatcatggg taaa
//
LOCUS       DAWDXZ010000040         6106 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3811_length_6106_cov_7.028921, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000040 DAWDXZ010000000
VERSION     DAWDXZ010000040.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3811_length_6106_cov_7.028921"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    737..778
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2087..2096
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1534 a         1350 c         1550 g         1620 t
ORIGIN      
        1 ggtgtgcgtc ggttcttgtc tgtgtatgat tatttcaatc aatgtcgcca tggagtattt
       61 ttttgagttc ggttaccgca atgccgatcc cgggcgtatt gccgcccagg tcgtatcggg
      121 tgtcggcttt atcggtgccg ggacgatttt agcgaatcag aaagaacgca gtgtccgcgg
      181 gcttacaacg gcagccggca tttgggccgt atcggctatc ggccttgtcg tcggtgccgg
      241 atatctggtg gtagcgattg tggcgacggc gcttatcttt ttggtcctga ccgtcttcgt
      301 ccgcttagat gcccgactgg aagggcagtt ccggaaaaaa tacatcatgc atgtcgtcat
      361 gaaaaacaat atcgaccaag cccggagact gtcccatttt gtcagcgacc atcagctcca
      421 tattaaaaag ttccacaatt acagtgacga tgaggctccc ctttcggaaa tagagttgga
      481 attgacggcc ctgtcccatg tggatgcgcc ggaaatcatc agtgagctct tggcggtgaa
      541 gggaatcaag gaagccgatt tttatgccga tgacggtcag aaaaaagccc ttgtcggtcc
      601 ttagagatac tgtatacaat atacttcatg tgtatacagt attttgcgcc cttttctata
      661 taaaaatagc ccttccggtg ctataatgca gagcgttgga acctttagat gtgctaaagg
      721 ggtaatggat agagagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta
      781 tcggctgtta tgccggtgat gatgagaaac tggcctgtct gcccgtcgta gaagaaatct
      841 accgcgcctt gccaatgcgc gattttatca cctatgcgcc gcctttgggc ttagagtctt
      901 atcgtcaagg ggccattgac gaagcctttg aagactgccg tccgcaaggg tatgccgatg
      961 ccgtggcaac ggccggcggt accggagcga ttcacattgc tatcgccaac tattctgaag
     1021 tgggtgacgt cgtccttacg acgaactggc gctggaatat ttatgatgcc ttgtgccagg
     1081 aaatcggccg tcggctccgg cctttttcca tgattgattc agaagggcat tttaatattt
     1141 ctgctttttc agaagctgta tcacaggcct ttgaacaaca agactcgctg ctgatcattc
     1201 tcaacacgcc ggctaataac ccgacgggct ttgccttgtc cgacgaagaa tggaatcagg
     1261 ttctcgacgt ctgtcgcttc catgaaaaga gcggaaaacg actgagtctg ctcgttgata
     1321 tcgcctacat tgcctatgcc ggtgaaaaga ataaagtgcg ctcctttatg cagcagttct
     1381 cgaacctccc ggcccatatt ttttcgatgt ttgctttcag catgtcgaaa ggctataccc
     1441 tgtacggtca gcgggccgga gcccttatcg gcgtgtcgtc cagcaaggct gttatcgatg
     1501 aatttgccga cctcggaaag tattcagcca ggacagcctg gtccaatgtc aaccgggcgg
     1561 ccatgacgct gctgacggaa atacgaggcg ataaatcact gaaagcccgc ctcgatgccg
     1621 aacgcctggc ttttgccgat aagcttcacc gccgcggagc catcttcgtt gaggaagccc
     1681 gacgctgcgg tctgcagcac gttccctatc aaggcggctt cttcatttcc gttccgacgg
     1741 atcaatcgac tgccctttgt caggcccttc aagaggatct tatctttgct gttcctttag
     1801 ccgaaggcgt gcgcctgggc atttgttcca ttgccgaaag caagatgaag ggattggcag
     1861 ggagaatcaa gaaggctgtc gatgggcttg agggaatgaa taacctctcc atggccggta
     1921 aataattttt tacaataaaa gacaaaagga tgaccaaaat ttttgtgttg gtcatccttt
     1981 tcatttgcaa aattctctca gaacctgtat aatgtaagta taaatttata attatatact
     2041 atttatatga agttgtaaag attgtgagta gaacgggtaa tggacgnnnn nnnnnnattg
     2101 gcacggcata tggaacatcg gagcaacttt ttttattcca tatggagcaa agttcaagca
     2161 taggcagagg cccttgagct gcgtgtgact ttataaattg agaaaggtgg gacaactgct
     2221 gctgcctctg cagacataga tcggaagacc gcgtccgtgt aaggattggc aagtcttttc
     2281 acttcggggg aaacaactgc atatggagga tacatctgcc ttcgttgcga acatcttgtt
     2341 ataggacaaa agctctcctg tattctgtgt tttagcagca gtgagctgca tgaatggatt
     2401 gaatcgattt atgggttgta tgttgggcgg agccgtcggc gatgccttgg gctttgtcat
     2461 tggtaaagac gatttaaaga cgattcataa gaagtacggc ccttatggat tgcgcacggt
     2521 gctaaagtcg gcaaagaacg gcaataagag tatcatttcg gatgatacac agctttcctt
     2581 atttacggct gacgggatgc tgtgggccga tcacgacgga ctggaaccga cggaaggttt
     2641 gtaccgctct tatatgcgct ggtactatac gcagacggag cgcatcatcc gcccggaaca
     2701 ggaaaaatgg atgaatcgcc agtcccatga agtcgcactg gaatacgaca tgatggggga
     2761 acaggcttta tttgcccgcc gctctcccgg gaaaacttgt ttgacggcct tggcgtcagg
     2821 gaaaaaattg tcccgtgatg aaaccatcaa tcacagcagc ggcaacagta cggtcatgcg
     2881 ggcagctcct atcgggctct tttatgaagg cgatccggag aaggcctttt ttgtcggctc
     2941 tcaggctgcc agtctgacgc acggcaatcc caaagcattt ctgtcgacgg caacgataag
     3001 cgccatcatc gcttctttgg cagtgggcaa ggatattagt acggctctcg gcggggccct
     3061 ccatattctg cagcgtgagc cagaggggac gcgtcttttg aaattgctcc tgcgatctat
     3121 tgatgaagcg gttaccgacc gtaatcccgt ccggtctatg aagaaaatcg gtctcgggaa
     3181 aaaagccgat gaggctttag ctttgtccat ttactgcgta ctcaagacgg gaaccttgaa
     3241 agaagcggtc ctcatggcct gcaaccagga cggcgacagc gatacctgcg gggctatctg
     3301 cgggaacatt atggggacca tctttggcga taaggctatc cccaagagct ggagctgcaa
     3361 tttggaatgc gatgtcttga ttcggagatt gtcgcagtgc ctttatgaac acaatcggct
     3421 gcagcaagtt gcaagtgaag ctgtcagcca ctgatatttc ttctgaaggc tttttttctt
     3481 gacattatgt gtgcatccca tataatgaga ttattattct tggcgtcgtg atgatgccgg
     3541 atcgggggag cgcttatgat tgaatccatt gaccgggcct tggccgtttt acagctcttt
     3601 ttaaaggaag agcggcccct cagtgtaacg cgtatcagca aagtcatgga actccataaa
     3661 agtacggtaa gccgtacgct ggagacgctg gaacaccgcg gatttattca aaaggatgaa
     3721 gaaacgggca agtactggct tggactgcag gcgtacgcct tgggaatgtt gtttcgcgaa
     3781 aaggataaat ttcataaagt ggcctatccg tatgccaagg ccttgtccct gcaatgcaat
     3841 gaaggcgttc atatcacgac ctttgcccat aatggcgcct cctatccgca gcacatcatc
     3901 ttggaaaaga ttaagtctcc caggaccatc gatacggcac cgcctatcgg cgccgtccgc
     3961 ccggcctact gctcggcatc gggcaagtgt ttgctggcct tttcagagga ctaccgaaag
     4021 gcttatacgg gctgtacctt gaaaaaattt acagaatata ccgttactga ctggaaagaa
     4081 ctcttgccgg cattggacga aatccgcaat cgcggctatg ccatcgaacg ggaggaagtc
     4141 gagctcggca tggcctgtgt ggcggcaccc atctttcgca gcggtcgcat tatcgcggcc
     4201 atcagtgttt ccggaccggc cgaacgagtg gcagatgcga atcgggagac cacgattcag
     4261 gctgtggtca agacggccgg tgatatcagc caggccttgg actgaggacg gagtcatttc
     4321 tcttgcgcaa atgtaaaatg ttcttcgatg tgaattgttt ggtataaaaa tcatgcgaaa
     4381 cgggaatata ctacatgcat ttgcagaaat agacttttat ggtacaatag gcatggatac
     4441 tacattcggg tcacaagatt gtctgttgtg aaatgaaaaa atctttcctt ttcaattgca
     4501 gcagtgtggg aacagcttgg gatcttatcc atgattcgag gcgaaaaaga atgcaaacta
     4561 caatcagtat ggctgcaacg acagtgccac gtgtggccta cccatataaa acgcgcaatg
     4621 ggggcgctac tgttaccatt tttgtgcctt acaattgcca taatcattgt cctttttgta
     4681 tcaataaggc tgaatatgaa gacatgaccg gcttttcgga agaagccatc tgccggagta
     4741 ttcgcttgat ggacagcatt acgccttctt gtgattttgt ttttacagga ggcgaacctt
     4801 ttgctgatat ggaatcactg cagcgcatgc tggactgcat tcccgtgaca cataaggtat
     4861 atatcaacac cacccttccg gtttttgaaa cacagacgga agacgatatc gtcgctttta
     4921 ctcagcggaa tcgtcataaa attacgtgca ttaacgtttc tcgtcatatg cagcattatg
     4981 tggaagaatc gaatgatcag cttctgacgc gattggccgt tcctttccgc attaactgcg
     5041 tcctgtataa gaagtacccc gtcgatcaga taaaaccgta tttggaacgg tttattaaac
     5101 tcgaaggtgc gtcgatccag ttccgctttg actatacgga aacgacgccg gaaaacttgt
     5161 acgaagaaga aaatgatcag attcttcacg acttaaaaaa tattgccgag tatacaggcc
     5221 tggacggttg ccgtatgcgc tgcggttttc atttcgatta tcaagggatg gaattgacct
     5281 accacaagac cctgccatac agcacggtca ttgaacggga tgaaaaagac ggaaagaatt
     5341 acgatatcct ctatgacatc ctgatcaagc agaatggtgc cattcattct gactgggatg
     5401 ggacgcccct tgatcttgaa gcttaccgta aagtcgtctt tgaaccgtat gatctccgct
     5461 ggattcaacc gtcgcggacg gggattggca aacatctgcc tttcttgaag accgcttgcg
     5521 ctgtttttca ttaattgaat atttttgtat gaccgataag ggattgctga gcggcagcag
     5581 tccctttttt tggctctttg tcaaatggtg gggtggccaa ctaaatcatc caacgcgaat
     5641 ggacgtgagt gagtttttct tgctctcgtc catttttttg ttagggataa gaggacgttc
     5701 acagcaaaat cttttttatt agatgaatga acaatctgac aatggcatat gataaaaaat
     5761 gcaagtgagt aggttgaggt tggaaaattg ggatagaaaa attttactac cgtgttatga
     5821 ccataaaacg gtagtgttta aggtccttgt ttcagatgtg gggagtgaat tgctaaatca
     5881 aggaaaactt tctggaaatt atgatttgct atatggggcc atattcgtcc ttttgataag
     5941 ggacgaatga aaaacatagc ggccttatct gacgcttcat ctctaattga attgacttag
     6001 cgaaagggac tttacggaca gggacagatg tcggagacca tattaatgac taaaatgaaa
     6061 gatattgatg ctatcttgat tatattttat aatatcctat tctttt
//
LOCUS       DAWDXZ010000041         6034 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3849_length_6034_cov_7.457936, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000041 DAWDXZ010000000
VERSION     DAWDXZ010000041.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6034)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3849_length_6034_cov_7.457936"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3174..3183
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1604 a         1575 c         1470 g         1375 t
ORIGIN      
        1 gaaaaaggac ctgtcaccta ggtggcaggt cctttcttgc atactcgatc agatagaaaa
       61 ataacgcacg gcttatacgc ggacggcaac gtggtctttg aattcatcca tcatagcctg
      121 acagcgtttt tcatcattgc ctaagagaat ggccaaacgc cccatgattt cagcaccgcg
      181 aaggatgacc ggttcaatgg acggatcttt tacggcatta gctatctcga cggagtgttc
      241 cggcataggc acgtcgccca atgctaaatg aaggtggagg gccgggcact ggtggctgac
      301 gttgcccaca tcggagctgc cacagtcctt cggcgcttcg taggcgattc ccatatcgga
      361 caggacttgc tccgccgcct tagttgctgt ttcattccag accatattgt cgaatttgca
      421 gcccatctgt ttaacgtcat acgtcgtttc ggttgcaatg gccgcccctt caaagcattt
      481 catgaccttg gcaacgaccg tatcgaggta ggctcgttcg ctgtggcgga tacagcattc
      541 cacttcagca taatcaggaa cgacattcga agctctccca ccgtcgacga tgtacgaacc
      601 gattcgcgtt tccggccgga cgtgctggcg cagcatatcg atcccgtgaa gggccagcat
      661 ggcaccattt aaggcatttc ttccgttcca aggttcgccg gcagcgtgtg ccgtctgtcc
      721 atggaaggca atgcgatagt catcgagggc caaaaagtga gagttggcat tggtcttggt
      781 cggcgatata tgaaccatca ccgccaagtc atagtcgtta aacacgccct gctcacacat
      841 gatgacctta ccgccgcgca attcttcatc gggtgtaccg atgaggtcga cgtcaacggg
      901 aagggccttc agttccttta atgcggcagc ggcaagaaag ctcatggccc cgttggccga
      961 atgaccgcag ccgtggccga cgccggggag ggcatcgtat tccgcaagga gagcgatttt
     1021 aaggacagcc ttttcagacc gggacacggc ggcccgaaag gctgtcttct ggcccgtaaa
     1081 atgttcttct acagccatgc ccaaactccg gcaggcatcg atataatgct gacaagcatg
     1141 aaattcttcc ccggacagct ccggatcatt gacaatggca taattcaatt ggaaggcctg
     1201 ttccttatgg agatccaagg ccgatttcaa agtagaaaca tgtttcataa taacgagaaa
     1261 ctccttttat ccgacgataa acatatataa ggggatgacg acgatcccca tgacaacggt
     1321 gataaacgtg acctgcgtcg agtaacggac atcggctccg atggacttgg caacgaccgt
     1381 catctgggtc atggccggca tactggcctg catggtaaag acttggcccg acataggcgt
     1441 tacgggaatg aaaggcaaga gggccagtac acaggccgga cagacaagaa atcgaccgat
     1501 taaagcaccg acgacgtcgc ggtcccaatg gatatcagaa aaggaaatcc cggccatttc
     1561 aatgccgatg accaacagcg acaacggcgt tgccatattg ccgacatatt ggaaagacgt
     1621 gagcaggaag tccggcagtt taatatttgc cagaaccaaa gccaggccga tcagaaagcc
     1681 catgaggggc ggagaaaaaa cctttttgat cgtctgagcg gaaaacaggg gcatattccc
     1741 cttcccgccc tgactgtcat tgacgatatg gtaaatgccg agagtccaaa acatcgtcgt
     1801 attggccata taatagagca tgaccgaggg cacactctgc gtaccaaaga gggccaaatt
     1861 gaccggcagg ccgataaaaa tcgtattggc gataaagaaa caggtgacga atacgccctg
     1921 ccgttcccgg cgaatatgca gcaccttggc agcaatgcgg ccgacgatat aggccaggaa
     1981 tatcgaggca aagggcaata tgagatccgg ggcaatacga atcaaagagt catgggtaaa
     2041 atccttttcc atttgacaga gcatatacag gggcaggctg attttcatga ccagttttga
     2101 aaaaacagcg ctcatattcg agccgaacca cccttttttt gacagtataa cgccgatggc
     2161 gatgataaat aaaacctcaa aaattccttc taatccatgg agcacgcctt ccaaaaaatc
     2221 gcttcccttc tacaaatggt ataactccgg ccgccggtca cggaagacat taatccggct
     2281 gcggaaacaa tcgacgatgg acaaatcaat cgtttccgta aggacggcat cgtcactgcc
     2341 gccttccacc aaaacattcc cctgaggatc gaccagcagt gaatgaccgc cgaatttaag
     2401 tcggccacag aagccgcagc tgtttacagc ggcaacgaaa aactgatttt cgatagcccg
     2461 ggcccggttg aggagctgcc agtgttccag ccgctcttcc ggccaggcag ccggcacgaa
     2521 gagaaccttg gctccggcta acgccgccat gcggacccat tcaccaaagc ggacatcgta
     2581 gcagatgatg gaagccatag tcacgccgtc gagagaaaaa acattgagtt ccttaccggc
     2641 cgtaaaggcc ttatcttcct tgcccggagt aaacaaatgg cacttgtcat aagaagaaac
     2701 aaggctgccg tctcggcgga caacgtacgt cgtattataa aggttccctt catgacgacg
     2761 ggcaatagag cctcctacga tatgtatatt acaagatttt gccagatcct gtaaaaaagc
     2821 ctgtgccaac agcccttctt catccgcttt ttctcggaca tcggagggat aaaaagacgt
     2881 atcccataat tccgataaga cggcgatatc ggcaccgtcc gcagctgcgg cctgcacatg
     2941 gcggcgtgct tgttcaaagt tcgcttcccg atcgccgggg acgatggaaa attgaatcag
     3001 tgaaattcgc ataatttcag ctcctttcaa aacgtacaaa cagcattggc atccaaccat
     3061 actagcattt aaagataaat atattataga ggatgatctc aaaaataaca attccttgca
     3121 aatccccgct tcccttgtta tagttaaatt actttaatac gcaaagaagg ggtnnnnnnn
     3181 nnnatcttgt catctgagac aggaaggtaa ccgcggaccg acgcgatata caggaaatat
     3241 cacatcgccc ttgatgttcg tcggcgaagg ccccggcggt gtggaagatg aatacggcgt
     3301 tcccctcgtc ggcccgtcgg ggcagctcct cgacaaggcc ttgtggagcg tcggtttgac
     3361 ccgcgaccgg gtatacgtca ccaatatcgt aaaatgccgg cccaagaaca accgtacgcc
     3421 aaccttagac gaaggccgtc actgtgccga catccactta atcaaagaaa tcgacatcgt
     3481 gcagccaaag gttatcgtct gtctgggaaa ggtcgccttc caatacttct acggcaaggc
     3541 agccagtatc atgcgcaatc gcggcaagtg gtttacctgg cgaggatatc ccgtcatgcc
     3601 gacctatcat ccggcctttc tcctgcgtca gaccgggcac gacctggtcg aatcgaaatg
     3661 gcaggtctat tacgatttca aggctgccgt tgaaaaagca aaagctgaaa atcccgatta
     3721 cacctaccag agcccggaaa aaacgaatct cttagaacaa tacacaccac tgaaagaaag
     3781 ccgaagtttt tgattcaagc caaaaagcac tgccatcatc gcgtgatggc agtgcttttc
     3841 gtttgtcctg taggtttgct gtacattcgt aaaaactacg ccagctcagt caaaatgccg
     3901 gtcatagaga tgactgtttt ccaagaattc ttcaaccgtc gggaagccga gtttctttaa
     3961 gagttcgatg acgtaaggat tttccatagg agtacgggag aaggcctgtt tcccataagc
     4021 ctggacgtta gccttgccgt cttccttttt gatgatggca cgaggaccgc cgacgcagcc
     4081 gccgatgcag cccatgcctt caaagaagtt tccgtcgcgg ttgccttcca aaatgtcgtt
     4141 aatcatggcc ttacaagccg gaacgccgtc agccttgcgg gtcttgatgt cgaccttgcg
     4201 gtcgggatcg agctggtgca ccgtcgtcgt gaccgcttcg gcaacgccgc ctgcataggc
     4261 atagctgatg ccggcttcgg aagcgtgagg tacgttgtct tccggcaggg attccaaatc
     4321 gatatcggtt acagagaaga aatcctttac ttcctgatac gttaagacgt ggtccacagc
     4381 accgaccaaa tcgggttcat tccgttccgc ttttttagcc aggcagggcc cgataaagac
     4441 tgtcttagcc ttgggatgaa gggcctttac ggtccggccg ccggcaatca tcggcgatac
     4501 ggatccggga acgttcggca agagctgatg atagagcttt ttaatcatag caatccacat
     4561 ggggcagcag cagctcgtca gctggaactg atcttcactc tgaatgttgc gatcgaattc
     4621 caaggcttct tttaaggtca ggatgtcggc aaagacagcg acttccaaca tgcccgtaaa
     4681 gcccaagcgt ttaaaagctg tccgcagctt gcccatggtg acattaggcc caaactggcc
     4741 ggcaacagcc ggtgcgacca tggcataaat cggtccgtcg tattcgtgaa gatcctgaac
     4801 gacgggaatg atgtccttgc gggtcttcaa gttgtcgagc ttgcaggcat cgacacaggc
     4861 ctgacagccg acgcactggc tgtcgtcgac ttcaacgcct tccgggccgc gtttgatagc
     4921 cccccaatcg caagcatctt cacagctgtg cggttcatca ggagggcaga ggcagggttt
     4981 tactcgccaa accagcggat ggtcatcggg atgagctaaa caatcgatca atctcggttc
     5041 cccttcaacg ccaataggtt tcccatcagc tgctcgtttg agcatttgct tatataattc
     5101 atctaatgtt ttcatacata aaccccctta aagagaataa cgaagccaaa tcagttagtt
     5161 tcattataac agatttttaa gggggctgac agttttttta ctgaaatttt agtctaaagt
     5221 caaaagtaat gccattttaa acggcccatc ggcagttacg gcatggcggc cgtttttcgc
     5281 aaaagcaaaa ttttcaccgg ctttaatcgg aaaatccttg ccttcatagt tgataacagc
     5341 cttgccttca agggcgaaaa taagagcttc acccggggcg gcgtgttcgg aaagacccgt
     5401 tccggcccca aagctcatga cgacgaattt catcttcggc gactggatga tgtcgcggtt
     5461 gatgatttta ccgtcctggc aggggacgag atcggccaaa gaaaagagtt ttccagtatc
     5521 gatattcata agagattcct cctcaatcgt aatttcaata tagacggcac cgccctggct
     5581 tctcataccg acggcaacac ctgccggtac ggccacgacc tgccctgaag ggacttccct
     5641 atccccaacc tctccataaa gggacaggct tccggaaagg acataaacga ccttggcctt
     5701 ctgatacgtt tcaggactga tatccgtatg agcggccaaa gaaaaataaa acaaatgata
     5761 gtcgcgttca tgggcgactt ccttagaaat cgtgcagcct tctacaggca gattctcttc
     5821 cgggatagaa aaaacaacac cttttcgttc attcatcatg tatccctcct tcttatggct
     5881 atagtatacc ttttgagtag cgttttagct gttgtaaatc caacaaaaaa agaagaaaat
     5941 agaatgtccc ccaaaacaca tccccctgcg acccatctat agaatctgaa atcaaagctt
     6001 tacaaaacgg gactttactg ttaaactgat atag
//
LOCUS       DAWDXZ010000042         5963 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3884_length_5963_cov_10.598341, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000042 DAWDXZ010000000
VERSION     DAWDXZ010000042.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3884_length_5963_cov_10.598341"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1394 a         1432 c         1568 g         1569 t
ORIGIN      
        1 ggtcgcagtg gatttcatca ctgatgatcc ataagtcatt ttcttccacg atatcggcaa
       61 ctcgctttaa ttcgtcagcc gtccatacgc ggcccgtcgg attatgagga ttgcaccaga
      121 tgaccagttt catcgtagga tcggcggctt tttgggcaaa atcttcaaag tctatcgtat
      181 aatgaccgtc ttcatcacgc cgcaagggag agcagacata ttctttgccg gaatattcag
      241 ccgcatgctg aaagaagccg tacgaaggcg tcgtgatgag gaccttttca tcgtctttta
      301 caagattttc gacgatttca tataaggccg ggataactcc ggaagaaaaa gttaattctt
      361 ctttgggaaa ttcccaatcg tacatctttt tgcaccaacc ggaaaagacc ttataataag
      421 agtcatcata caggccggta tagccaaaaa tccggcggtc gattcggtct ttcatggccc
      481 ggcaaatttc cggcggcgtc gcaaattcca tatcggctac ccacatgcgg ataaattcgt
      541 catctttaaa gggaaatttc tttttccctt ccacgtcctt gaaaatatag ctgcggaagc
      601 cgtcggtatt taaggcccga gtcccccttc tatcaatgat ttcatcaaaa tcgtaatgca
      661 ttacaaacac cccttctttc ctccaaaacc ggaaacaacc tgtttccgcg gacaatatgt
      721 tcgatgaaca aagtatatca aaggccttgt gtgtcgtcaa aaaggtattt tccgcttaag
      781 tgaatacgat tccgcaaaaa aaaaaagacc ctgcactatt ccgaatgcag ggtcttcatg
      841 taagcggttg ttatctcata ctatttattt ctatacgtgt ttcaaaagga ggagagtttg
      901 gttaggaaac tctttgatta tctgaggatg gcaccggaga tgatcatctg ctgtacctgg
      961 ttggtgcctt cataaatctg agtgatctta gcgtcacgca tgtagcgttc agccggatat
     1021 tcacgagtga agccgtagcc gccgaatacc tgaacagcat cggtggtaac ctgcatagct
     1081 acgtcagaag cgtacttctt agcgatagca gcatcgacgg agtaagattt aacatcgggc
     1141 tgggccatct tccaagcagc ctgatataca gcaaggcgag ccgtttcaat cttaagtttc
     1201 atatcggcga gcatgaagct gatagcctgg aatttaccga tcggtttgcc gaactgcgta
     1261 cgttcttttg catactggag agctgcgtta taagcgcctt cagcgatacc gagagcctga
     1321 gcaccgacgc cgatacggcc gccgtcgagg gtcatcatag cgatcttgaa gccttcgcct
     1381 tctttaccga ggaggttttc agccgggatt cttacgttct ggaatacgag ttcacgctgt
     1441 acggaagcgc ggatacccat tttaacttct tttttaccgt aggtgaagcc ttccatgcct
     1501 ttttcaacga tgaaagcgct catgccttta gcacccttcg atttgtccgt cgaagcaaag
     1561 acaacggtga tgtcggcttc gccgccgttg gtgttgaata ctttagaacc ggtcaaggtg
     1621 tagctgccgt cttcgttctt aacagctgcc gtagaaccgt tcagggcatc ggtaccggcg
     1681 ttcggttcgg taagaccgaa agcgccgagt ttcgtgcctt ctgccaaggg tttgaggaat
     1741 ttagctttct gttcggccgt gccgaatttc caaatcggcc aggagcagag catggtatgt
     1801 acttcgaagc tgagggcaat ggaggggtcg actttagcga cttcttcgca ggctacaacg
     1861 aggctgagga agtcagcgcc aacgccgccg ttttcttctt cccagggaat accgagaagg
     1921 ccgagggtgc ccatttcatc gaggatagcg cggtcaaaaa cttctttttc atcgcggtct
     1981 tttacagtag gagctaattt tttttcagca aaatcttttg cgagattggc aatatcttgc
     2041 tgtaattcag aaagtttaaa atccatgatt gaatttcctc ctaaaagatt atatgtatat
     2101 ctttatattt aaaaacggcg aggccgtctt taaagcaaca ttgatagccc aaagcccgct
     2161 agaaacgggc ttcaagctat gcatcctgcc agggaagaca ggttatattg tggccggctc
     2221 ctcacggggt cccgcccaag tgaaaccaag gcttagagaa tttccgagaa ggcctggata
     2281 cgcgtgcgga gctgttccag agacggagat tcaatatcga ggtcgaggac ggtgtagcgg
     2341 acaccggctt cttcaaattc cggtttgtaa atcggatagt caaattcttc aggatcgcag
     2401 aaacgcatca tgcagacgac gacagcgtcg gccttgtatt tcttggtcat gtcgatgagc
     2461 atctgtccgc gcggtttgtc cggattcagg gccaaggggc agccgtcgaa atcctgccac
     2521 tgctgagcga gctgttccaa gggatcgatg ccggacggta catccgtacg gaactgacgg
     2581 gattcctgag ccaggtcgtc ggcaacgacg gcaatgttga attcgctgaa gatatcgagg
     2641 aattcatccg gttcggccat gatgcccgaa aggacgactt tcttgccatt ccacggctgt
     2701 accggttctt tcttgacggc gtccatgaac caacgagctt tgatgacgtc gtgacgtttt
     2761 accggcgtga agatttgcgg atactgagcg gcaacgtcgc agaattcgcg catagcacgg
     2821 cggttttcgt tgtatacgtt gatggcttcc tggatggcaa ggtcggaaat cttgacgccg
     2881 aggatgcgtt ccaattcagc gcggacgtgt tcgtattcgg ctttgaggaa gtcgacggcc
     2941 gggcgaatct tgcggttctg cggatgggta aatacgatga ccggagcctt gccgtgccat
     3001 ttctggctca tgcagcgcaa ggtgtcgcag ggtacggaga agatgacggc atcgaggcag
     3061 tcataagtac cgttgagctg gagttccata tcggcctgca tcaaggaaca agcaaaggga
     3121 ggcagatagg tacgggcagc ggcaatctgc ggtttctggg caccgaacat accgacaggg
     3181 agacagccgg ctgcgtatac gatttcttcc ggggcatagt aaggcatcat gccgaccagg
     3241 cgtttgccct gacttttata gtttaaaacc gtcttttgag gatggttgga tacttcttga
     3301 aatttgctga taagttcatc aatctgactc attagtgatt tcctccttca ctggcttttt
     3361 tatctgccat catttcggtc aaagcttcga cacgggtatc gaactgagct tctgagaagt
     3421 ttcgggcatc ggtctggtca ccgtcgaaga tgacatacgg tacgccgagg ttcttctgaa
     3481 cgcgctggcc gccttcgttg ttgaggaggc tcatgttctt gcaggaacgg ttctgatgca
     3541 tgatcaggcc gtcgcatttg ccgtcgcgga caactttttc aagaacggca ccgcgctgtt
     3601 cgaggcagca gttgatatag gttcgggaat aagcttctgc catggattcg aggttgcccg
     3661 gttcataaga aacgtcccac atgccgggat aagcagaacc ggtcatgaga gcgccctgac
     3721 ccttgagttc tttaaaggta tggccgagag cgatccaaac ggcaatgcct tcccaggtaa
     3781 cacgagattt ttcattgtcg ccgaaggccc atttcttatt ggcaactttt tcatccaatt
     3841 ccttgaggaa tttgttgaac gtgatttcag agtagttcaa agaacgagcg gcaacggcga
     3901 gacccatgta gttgaagagg tcaaaaccgt tgagcggcga cggtttgtat ttgaagtagg
     3961 tcgcaatctt gttccaggca gcgatggagc gctgagtctg tttctggact tcaaagaatt
     4021 tttcatagtc aaaggggcgg ccgcagagtt cttcgagctg agaaatagca tgtttgaatt
     4081 cgccgacgat gtactgttta gcgtgtttcg taaccgggaa ttcatggttg aacggtacgt
     4141 cgatgttgat gagaggaacg ttcagttctt tggccaagtt ttcataccat ttgaggaggg
     4201 tattgcaaat attgttgcac gtgaggacga cgtccggaag gggaatggga gaagccggag
     4261 aattcttcaa gacttccgga gttttgcccg taagggcttc ctgtttgatg agttccatat
     4321 agcccatgtt gacgcggcag taggaacaga tatcctggtt atagccttta ttttcggcaa
     4381 cttcgagcat agccggagca ccatgacggg caccgatgcc ggcagcgtga gtttccggat
     4441 agacgatggc aatatccatg gccgtgcaga cttccggagg tgctaccgag gcggaccagc
     4501 aaacgaggcg gccttctttt ttagctttgc gtgcgtcttc atcgactttc ggcaagaagt
     4561 aaccaagggc gtccttggcg ctcatgcttt caatatctac tgttttttct tcactcatga
     4621 ttctgttctc tcctttgact tatttcttga ttttatcata agcaatcaga gcggctccca
     4681 aggcgccaac ggcctgagga tggagggcta ctctgacagg gatacctaat tctttcgata
     4741 cggcatcgac gacaccgggg tcgcgggaac cgccgccggt catggtcagg tcaccgccga
     4801 caccgacgcg gcgcacgagg gcgcaggcct tggcagcaat ggactgatgg acaccggcta
     4861 cgatgtcttc tttagggatg ttcttcgaca agagggaaat gacttccgat tctgcaaaga
     4921 ccgtgcaggt actgctgaca gccgccggat gcttcgattt gaagtaccag tcccccattt
     4981 cagacatggg gatttccagt accttggaca tgacgtcgag aaaacggccc gtaccggcag
     5041 cgcatttgtc attcatggca aactgcatga ccagtccctg actgttaagt ttaatcgact
     5101 tgacgtcctg accgccaata tcgaggatgg tcgtcgtgcc cgggcattcg aagagagcgc
     5161 ctttagcatg acaggtaatt tcgctgactt cgcccttggc gcagtcgacg ttgaagcggc
     5221 cgtaaccagt ggcgatgata ttggccatat cttctatctt taagttcgtc ttttcaaaga
     5281 cttcgtccaa gacccgttcc ggaccggatg aaccggtacc gatttcaacg acggcttggg
     5341 caatgatttt cttgccgtct tccaaaatga cggctttcga agaagacgaa ccaacatcga
     5401 ttccgagagt atacatgatg gtcacctttt ttcacaaaaa tcaattctga aacggattgg
     5461 gattgctgcg attgatgggc gcaacggttt ggacgtattc gacgaggata ccggcgtccg
     5521 tgctgggacg gcggaagttg acgacgatgt cgtccgttcc ctggacggct ttcttttcca
     5581 gcatacagcc ttcttgctgg ccttccatga tctgccgtac tttttcgacg tcgtcgactt
     5641 caaaggcaat gtgggcaata ccgccttttc cgtgattgaa gtccttcaat accccttccc
     5701 gagggatgat gaattcaatg ggcgtactgt ttttatcttt cttcgtaaag ataaggtcgg
     5761 catggtaggc atcgacgaag ccggcatagt cgacttcaag gccattcgtt tctatgaaat
     5821 catgggcctt ttccaaggtc ggcaggacga tgccgatatg gtggagccgc atgggatgcc
     5881 acggttcacg gagctttcct ttttcagccc ccatggcctt tacgaaggga tcttcgccgc
     5941 ggcctgtaat gggtgccgtc gtc
//
LOCUS       DAWDXZ010000043         5941 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3896_length_5941_cov_8.752633, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000043 DAWDXZ010000000
VERSION     DAWDXZ010000043.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3896_length_5941_cov_8.752633"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1570..1688
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5179..5188
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1414 a         1761 c         1478 g         1159 t
ORIGIN      
        1 ataacttccg atacagagcc ctcgtcccag tgtcgaatcc ggcagatagc gcttcatatc
       61 ggctatcgta tcgtgtaagt cgctgccgcc ggaggtcgca aagggaatga ccgtcttccc
      121 ggaaaaatca taggcttcat aaaaagaccg gacgaccatg ggctcgcggt accaccagat
      181 gggatggccg atgaacacca catcgtagtc gtccatggcg gctacctgag ttttcaaagg
      241 cgggcggata tcgtcattta cttcctgatg agcctgacgc gaacagggat cgtggtctcg
      301 gggatagaaa cggtcagttt ctatttcaaa caagtcgccg ccggtctgcc gggcaatttc
      361 ccgggccacg gcgcgagtat ttccgctatg ggaaaaatag gcaattaaaa tcgtatgctt
      421 catcgttgtc cctccttctg acaaggttat tatgccacgc cttgccttga ctgagaatcg
      481 tcaataagtt accatagcat taaccaaaag tttatggagg ctatgatgga tattcaaaac
      541 ctgcaaactt tcctggctgt cgccaagctg ggaagcttta ccaaggcggc cgaacagaac
      601 ttcatttccc caacagccgt catgaagcaa atcaataaac tcgaaagtga actggactgc
      661 accctcttag agcgatcgtc agccggcatc cgcctgaagc cggaaggcga ggttttcctc
      721 acctatgccc agcagctgat tgagctctcc cacgaggcct atttggcctg ccataaggcg
      781 acgaaccaag ttttcaccct acgcctggga acgtccctcc tccatcccag ccgccccttt
      841 ttatcgattt ggaaccgcat caaggacaag ctgccccaat acagcctgac catcgtccag
      901 ctcccaagcg acctcgtcac ccaaaaccgg gaatacgaag ccctgggaaa gacctgcgac
      961 atcctcatcg gcacctttga ccaggcgacg acgcacacct tggtcgacgc cgttccctta
     1021 ggctcctatc gcttcagtat cgccgtccgg tcggacaatc ccttagccga aaaagacctg
     1081 ttagccatca aagacttgga aaatcagcac ctgctcatgg tcccgaaggg catcagccat
     1141 aaaaatgatc tgctccgagc tcagatggag aaagagttag cccattgcca aattctcgaa
     1201 acaccgggcc gttacagtct cgataccttc aatgccgcca tcgataaaaa tgctgccctc
     1261 atcaacctga cgccgtggga agacatccat ccgggcctgg tcagcatccc cttggcgacg
     1321 gatatcaccg tcgactacgg catcttagcc gccaaagatg caccgtctcg ggtcaaagaa
     1381 tttctatcga ccatccgcta ccagctcctc acgcaaatgg gcgaatacgg tccggttaca
     1441 cctgtcgaag aaaatccctg acagccgact aaaacacgaa ctccaccgtc tcttgcaaga
     1501 acgtttccat ttttccatta ccgggagtga aaatcatgat tgataaaatg ctcattcgcg
     1561 gcgccaaggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnaa aacatcgacg tcgatatccc ccttcataaa atcgtcggca ttgccggcgt
     1741 ctcaggctcg ggcaaatcgt ccctggctct cggcgtcctc tacgccgaag gctcccgccg
     1801 ctacctggaa tccctgtcga cctatacccg ccgccgcatg acccaggcgt cgcaggccgc
     1861 cgtcgacgaa gtcctctatg tgccggcagc cctggccctt caccaacggc ctggcgttcc
     1921 gggcattcgc agcacctttg gtacgggcac agaactctta aacagcctgc gcctcatcta
     1981 ttcccgcctg gccagccacc gctgcccgaa cgggcactac gtccggccct ctttaaacgt
     2041 ggccgccgaa aaagccctcg tctgtccgga atgcggcgtc catttcatgg ccccgtcagc
     2101 agaagaactg gccttcaact cccaaggcgc ctgccccgac tgcagcggca cgggaacggt
     2161 ccgcaccgtc gatatggcaa ccctcgtccc cgacgaatcc atcagtatcg acgacggcgc
     2221 cgtcgcccct tggaacagcc tcatgtggtc cctcatgact gacgtctgcc gagccatggg
     2281 cgttcgcacg aacgtgccct ttcgcgattt gacggccgaa gaaaaagaca tcgtctacca
     2341 cggaccggcc gtaaagaaac acattttcta taagtctaaa aactccaacg aagcgggcga
     2401 actcgacttc acctactata acgccgtcta caccgtagaa aacgccttgt ctaaggtcaa
     2461 ggacgaaaag ggcatgaagc gcgtcgaaaa attcttgaaa gaagaagtct gcccgacctg
     2521 ccacgggacc cgcctgtcag aagcagccag agcgcctaaa gtacaaggta tctccctcga
     2581 tacggcctgc accatgaccc tctcggcctt aacggactgg gttcaagccg ttccggaatc
     2641 tctcccaaaa gacctgattt ccatggctga cagcatctgt gaggcctttc aaaacacggc
     2701 caagcgtctc ctcgacttgg gtctcggtta cctcaccctc gaccgctcgg cgggaaccct
     2761 ctcgacgggc gaacggcagc ggatgcagct ggcccgggcc gtccgcaacc ggacgacggg
     2821 cgtcctctac gtcctcgatg aaccgtccat tggcctccac ccgtcgaaca tcgtcggcct
     2881 tacgggcgtc atgaacgacc tcatcgccga cggcaactca gtcatcctcg tcgaccacga
     2941 cacgcagatt ttaaaagaag ccgactggat catcgaaatg gggccgaaag ccggcatcca
     3001 cggcggtcac gtcttggccc aaggaacgat agaatccatc gaaaaaaatc ccgtctcgca
     3061 gattggacct ttcctgtcag gcaaggccga gaccaaggtc cgcggcgacg tcccgccgga
     3121 tgagctcttc gccaagagtg agattcgcct ttctacttcg gccattcaca cggtcaagcc
     3181 cctggacctc accatcccca agggacggct gaccgtcgtc accggcgtat ccggttcagg
     3241 caagacgacg atggtcctcg aaagcctcat tcccgccatc gaagccgtca tagcgaatcg
     3301 tcccctgccg gctcacatta acagcgtcga caccggcggc atcgaacagg taaaactcat
     3361 tgacgccacg cctatcggca tcaacgtccg ctctaccgtt gcgacctatg ccggcgttca
     3421 tgacgaactg cgaaagctct tcggaaagtc acctgatgca aaagataaag gctataaggc
     3481 ccgaaacttc tcgtacaaca cgggctccct gcgctgcccc gactgcgacg gcacaggtac
     3541 catcagcctc gacgtccagt ttctcccgga tgtgcacatc ccctgcccga cctgtcacgg
     3601 ctcgcgctac gccccggccg catcgggcgt caaattgacc aaccaagaag gaatatcccg
     3661 gacgctgccg gaaatcatgg ccatggatgt cgacgatgcc gtcgctttct gcgccgaatg
     3721 gaagggcgtc tctcaaagac tcacaaccct cagccgcctc ggtctgggct acctgactct
     3781 cggtgaagaa acgccgggcc tttccggcgg cgaagcccag cgcctgaaac tggccagtga
     3841 aatgtggcga acccagacgt catccctctt cgtcttcgat gaaccgagca tcggcctcca
     3901 tcccctggac gtccaagtcc tccttcgcgt cttccaagcc ctcctcgata aaggggcaac
     3961 agtcgtcgtc atcgaacacg atctcgacgt cataaagaac gccgactaca tcatcgacat
     4021 gggccccggc ggcggcgaag acggcggccg catcacggcc acgggcacgc cggaagctat
     4081 caaacagaac aaagacagca tcaccggccg ctatttataa cacaaaaggc ctcatcactg
     4141 acttctttat ctttccaata gagaaatcga tgatgaggca tcttattttt tacagcaaat
     4201 tccatacaaa agaacctcca ccgatcaagt tgaccgatgg aggttctttt gctgttatct
     4261 gctatttatt tttggcagca aggcgggagt ttagatttat cccccgtctt tgactgtccg
     4321 ctcgacgagc agctgcccga gcagccgccg cagcagccgg ctgttccgtt gaacaggccg
     4381 cggacatgac ggatgaagta cgctacggcg atgatgacga cgatagcgac aataatcgtt
     4441 gcaggactca taccagaacc cccctttcta tgggatagaa ggtcttatgc gccgagaccg
     4501 gccataagag ctaactgata ggtaacgtaa gccaggacat aggccaagac gaggcacatg
     4561 gcatcgctgg cgaggagcat cttccaggaa cccgtttcac gcttgatgac agccatagca
     4621 gcgacgcaag gcatgtagag caaggtgaag accatcagcg acagggcaac agccggattg
     4681 aagttcgggt catcagccag gaaggtgatg aggttggctt catgagtatc gtcagcctgg
     4741 acgctgtaaa tcgtagccaa ggtcgaaacc atgacttctt tagcggccgt gcaggcaacg
     4801 atggcgacgc cgatcttcca gtcataaccg agcggtgcga tgaccggttc gatgaagtgg
     4861 cctaagcggg ctgcatagga ctgggaaatc ttttcagcat tctgctggct gtcgagttca
     4921 gtcgtagcat cgtcagcagc gactttggcg tcgaataagg gcagtgcctg agcatatacc
     4981 gtcgggttcg aagcttcgag atcggcaaaa gcatcgggat actgcggttt ttcttcgaca
     5041 gcttccgcct gctggacgct ttcaacatct tcttcttcgc tgtcttcttc ttcagcggca
     5101 gcgtcggcag cggcaatcat cgattcatac atgccgttca attcactgtt ttcttcgatg
     5161 gacgtaaggc cataggtcnn nnnnnnnngt ctggagaata ccagcctgct gctgttccat
     5221 ctgagccgta acctggtctt tagcggcatc gaagtcctga gaatactgga catccatcgg
     5281 atatgccgtg aggaaccaaa cgatgatcga agcgccgagg atgaaggtac cggccttttt
     5341 gaggtacagt acgcttcgtt cccacatgtg catgaggacg cctttgaggg tcggaatgtg
     5401 gtacggcggc atttccataa caaacggttc ttgttcgcct ttaaaggtcg tatgacgaag
     5461 aaccaaggca acggcaacag cgacgatgat acctaaaagg tagatgccga aaagaaccgt
     5521 accgccataa ccggaagcgc caaagaaggc gccgatgagc agggtataaa caggaagacg
     5581 agcgccgcag ctcatgaagg ggcacgccag gatagtgacc aggcggtcct tttcattatc
     5641 gagtgtacga gcggccatga tgcccggtac gttacagcca aagccgagaa tcatagggat
     5701 gaaagacttg ccgtgcaggc cgagagctct catcgcgcgg tcgataagga aggcagcgcg
     5761 ggccatgtag cccgtgtctt cgagaagact gataaagaaa tacaagagga cgataagcgg
     5821 tacgaaactg aggaccgaac cgacaccgcc gatgacaccg tcgacgagga gcgagcgcag
     5881 ctgttcatct tcaacgaccg tactcaacca gtcaccgagc atgccgaaac cttggtcaag
     5941 c
//
LOCUS       DAWDXZ010000044         5867 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3930_length_5867_cov_7.893840, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000044 DAWDXZ010000000
VERSION     DAWDXZ010000044.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3930_length_5867_cov_7.893840"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1248..1257
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1434 a         1507 c         1485 g         1431 t
ORIGIN      
        1 ataaggccgg caatataggc caccgtcaga ttatcggcaa aggcgacgcc taagaaaaag
       61 acggcataga gaacgctgcc aaaaaaagaa acgatacggc ggccaaagcg gtcggataag
      121 ggaccggaga agaaactgcc gatgaggcag ccaaggccga gagaagccga tacccagacg
      181 acgccactgg catcatagag gccgccggca agctgtgccg acccccattg ctgggccaaa
      241 gcttgtttat aatggccgag aatggatgcg gccacaccaa ggatcagata gttaagatat
      301 aatacgatag ctgtaagata caacttttta ctggtctttg ttacttccac gttctattcc
      361 tcctgatagc actttatgtc tcccaaatca aggattcggg agacacgttg ctattcgcaa
      421 taagtgctta gatttctaag gctgcattga aggcatcggt cgtcgccgta agggcgctgc
      481 caggtctgcg gcacgaattt agagacttct tcattgagcg ggttgtagct gcgggagccc
      541 atggccaaga cgatgctgtc gaagccgcgt aattcgcctt tcgttccgtc agccatttca
      601 tagttgacgc cgtctttaaa gaattccgta actttagcgc ccggtacgag ttctacgccg
      661 tggcgttcga aatcgttgaa gatgaagacc cggttttctt tggaaacgtc agcagcgagc
      721 tgctctttca tttcgatgac cgtgactttg tgatcccgtt cgcagaggaa ttcggcagct
      781 tcacagccga ccataccgcc gccgacaacg agggcttttt tgccgatttc atagcggccg
      841 ctcagcatat cctgtgccgt aatgtagtcc gagtcagcca ggcccgggat attcaggata
      901 agcggtaccg aaccggtcgc aaagatgacg gcatcgggag ccatgtcttt cagcagttcg
      961 accgtcgctt cggtgttcat cttaatggtg acaccggctt cttcagcgcg gacgatgtag
     1021 ctgcggatag ccgaggtcag atccccttta tcagccggga aagcggctaa gcggaagttg
     1081 ccgccgagtt catcgcctcg ttcgagaagg gtgacctgat gaccgcgctg tgcgcagatg
     1141 cgggcagcat agagaccgcc ggggccgccg ccgacgacga cgactttttt gcgcttgtcg
     1201 gccggcacga tttctgcttc acgaccgcat tcagggttca gtgcgcannn nnnnnnncgt
     1261 gaacggcacc gggagcccac atgaattcgt catggacgtg gacggcacgg ctgacatgga
     1321 gggcgttgac gccgcattct tcgacaagat atgctgcaac ggctgctgca tcctgaatcg
     1381 tctgaccgcc ttcaatgtca tcgctggcgt taatacggca gaggatcggc atcttgccgc
     1441 ccgtcttttt gcggatgttt tcaatgatca accgcggcag gcgcatgcgg ttttcaaagc
     1501 aaccgccgaa ttcgtcgaga cgattgttgg tacgggacga aatgaaggtg ctgaccaagt
     1561 aaccgtgggc acagtggact tcgaccatat cgatgccggc acgctgagcg cgggcagccg
     1621 catcgccata gcattcgacc aacttataga tttcttcacg agaaatagcg accggtactt
     1681 cgcggcccga cgagggagcg atggcactgg cggcttttaa gggaaagccc gtaagcgccg
     1741 agttgccttc cggaccggca tgctgaagct ggatagaagc aatagcgcca ttttcatgga
     1801 ttgcatcagc gactttgcgg aagctgtcga ctgtatcatc ggaaaagagg cacggtttgc
     1861 gcgggccgcc tttagcttca ctgtaaacga cggtagattc aatcgtaatc agaccgaagc
     1921 cgcctttggc acgagctgcg tagtaagcag ccgagcggtc gctcaatgta ccgtccgtat
     1981 tggcaaagtt gttgcccatg ggcggaacga cgaagcggtt gggaacggta acagaaccaa
     2041 tctgaatcgg ttggaacata gcgttgaatt tcatatcgat aatctccttg agtatgaata
     2101 aacttaagcg aagaattttt caaaaacttc ttcatgaggc atatccttgc cagtgaagag
     2161 tttaaaggca gctacgccct gccagaggag catgccgatg ccgccgacag ccgctttgca
     2221 gcctgcttcc ttagcatcca agatcatctg cgtatctttc ggattataaa ccgtatcggc
     2281 aacgactaag tcggagcgaa ggtattcttt cttaatcagg ctctggccgt cgaggggtgc
     2341 catgccgact ttcgtagcat tgaccaagat atccgttttg gcaatggctg ctttcaaggc
     2401 ttcttcatcg gctaaatcgg tgaccgaaac cttgcaggaa aggtccgctg ccgtaatctt
     2461 ttcgaccgtc ttttcggcat tggcaaaaaa ttcgtcttta cggttgaaga tggcaacttc
     2521 agctgcgcca tcgagggcag cctggcaggc aatggccgta gcggcaccgc cggcaccgag
     2581 gagaacgatt ttcttgcctt tgatgtcgac tccattttcg tggaggttat ggacgaagcc
     2641 gacgccgtcg gtgttgttgc cgatcaactt gccgtcctta acgacgacag tgttgcaggc
     2701 accgattaat tcagcagccg gagacagttc atcgaggtac tgagcaatgg ccgtcttgca
     2761 gggcatggta acgttaaaac cgccgatatt caaggtgcgg acagctgtga tgacgtccgg
     2821 cagctgatcg atttgtacat cgaaagccaa ataagccgca ttgatgccga ggcgggcgaa
     2881 cgtataattg tacatgaccg gggatccgga atgaccgacc ggtgaaccga ataaaccgaa
     2941 taattgtgta tgtccatcaa ttcttgcttc catgtttaaa actcccttct cttactatat
     3001 cacagccgat tagccgcaga tttcaggcca aacctgttct aatactttct ttgtgccttc
     3061 aaagttttct ttggcagctt ccttaacgcc tttagcaacg atgacatcgc tgatgacttc
     3121 gacgccgcag accttcggtt caatgccttt gtcgcggatc atcttgacga agcctgccgt
     3181 atcaccgtag cccgtaccgg gaatcatgcg gtcatgcatc gattcgtcac ggagaatagt
     3241 cggtgcataa ggatgctgct gaacgtcgtt gatctggatg gagataattt tatctgccgg
     3301 aatgtcggcc aggtacgacg gatcgtaaga ctggttagcg cggatccagt gccagctgtc
     3361 aaggatgagt ttggcattgt cgcagccgct gcctttgacg acagcccagc ccgttttgac
     3421 gtcggggata ccggagtacg gcatgggttc aacgccgatg atgtatttgc cggcacggtg
     3481 gcacaattct ttcaatttct gagccgtatg ttcaacggag tatttttcca tcaaaccgat
     3541 attgatgtgc tggacgttga agaggtcgca catatggaag cagatctgtt ctttatactt
     3601 ctgttcatac gagcggtggt cttcagccca catagtgatg tattcgactt ccgtaacctt
     3661 catatcgtat ttgtcgagga tggccaggat gtcttcgtca tgaaggcctt ccgctaaggc
     3721 atcgacatag gtttcagcac ggaggccaat gccttcaaag ccggcttctt tcgcagcgct
     3781 gacgcgttct tcaaaggtac actgatcgcc aagggtccag gaacttattg taatcgggga
     3841 ttttttagac ataatagata cactccttaa tataatataa ataatgtgtt atatattctc
     3901 atcgctgcct acgcagtaaa aagctattta tagagcaagt ccttaatttc atcgacgggc
     3961 atcgttccgc cggtataaag ccggtaggct tcagcaccct ggaagagcag catgccaagg
     4021 ccttcaaagg ttcggcagcc tgcggcttta gcctgtttca ggagcttcgt ttcagccggc
     4081 gcatagacga catcggtaac gatcaaatcg ggacgcagta cgtcgagacc gtcgatgatg
     4141 ctcttatctt caaagggcgc cattcccaag cgagtggcat tcgaaaggat atcgctgtcg
     4201 gcaatttcac gtttcagcag gtctttatca cccaaatcat ccaaggtgat cttaacgccg
     4261 ggcacgcact gacagatagc ctttacattg gcttcggccc gagaccagaa actatccttc
     4321 aagttaaata cggaaatctc ccgtacccct tctaaggcac attggatcat gatggctgag
     4381 gcaacgccgc cggcaccgac taaggtgatc ttcttccctt ttaccgacac accaccggtt
     4441 ttaagattga gcgtatagcc tgcgccgtcc gtaatgtggc cggtgaggac accgtcgtcg
     4501 ttgacgatgg tattgacgga accgactgcc ttggctgccg gcgatacctt atcgagaaag
     4561 ggaatgacgg cattttttaa aggcatggtc acattagcac cgcgaaagtt caacgttttc
     4621 agagaatgaa gggctttttc gacattgaca tcgaaagcaa aatacgcaaa gtcctgattg
     4681 aatttctgaa aacagtaatt atacatctgt ggagacttag aatgatcgac cggggtcccg
     4741 atgagaccga gcagccgggt atgtcctgaa atatgcacga ataaacgctt ccttttcata
     4801 agtaagtgta aaaatcaaag atgcggcagg ctccgttgtt tttcttcgac gcctgtcctt
     4861 cgatgggtct atggtaccgc attgtgaatc atttaacaaa tcaataattt tcatgttttc
     4921 atagactaaa actatcggcg atgataaaat ggtcttatat gtaactaaat gtaatagctg
     4981 cggcgacgac agttcgccag gaggacgacc atgaatcttt cccatctcag atatttcgtc
     5041 aaattagccc agataaaaca ctataccaaa accgccgaat tgctctgtat tacccagccc
     5101 agtctgagtc acgccatttc ccaactcgaa aaagaactcg gtgtccctct cttcgaaaag
     5161 gggcctcacg gtacggactt gaccggcttt ggcaagcagt ttttcgccag cgtcaattca
     5221 tcactcaata ttctcgacaa cggtatcgaa acgataaaaa gcggctcccg cggcaacgga
     5281 atcatccgta tcggctgtct gcggacactg gggatccgcg aggtcccgga actggctgcc
     5341 gcctttaaag cgtcccgccc ggataaggat atccgcttta ccttccacat cggcctgtcc
     5401 aaccgcctca tccaatcctt agcagaaggc accttcgacg tcgtctttgc atcacgcccc
     5461 cagaccatgt ctcaatttga agcgacggcc attgccaaac aggagctggt cctgattgtc
     5521 cccaatgacc atcccctggc tcaaaactat gtcgtcgacc tcagagacac cttagattat
     5581 ccctatgtct tcttcgataa gacgtcgggc ctgcgtccca tcgtagccca tatgtttgca
     5641 gaaatcaacg aaactcccaa aatagcctat gaagtatcgg aagatcaggt cgttgccgga
     5701 cttgtcgcac agaacttcgg catcgctgtc gtccccttca tggatatgct cttacagcta
     5761 gacgtcaaga ttctgcccat ttcccatcct tcatacgaac gaaaatttta tatgatttcc
     5821 aataagaacc aatatgcccc gccggtcgtc caagatttca aaaaatt
//
LOCUS       DAWDXZ010000045         5849 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3939_length_5849_cov_8.753711, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000045 DAWDXZ010000000
VERSION     DAWDXZ010000045.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5849)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3939_length_5849_cov_8.753711"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    224..233
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1248..1257
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3759..3768
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1311 a         1324 c         1669 g         1515 t
ORIGIN      
        1 gtgtccatgt tggacgtgtt gacgtagatg agttcgacgc cgtgttcggc tgcgtattcg
       61 ggggatacga cttctttggc tctggcaagg cgggcctcgt tgatatcggt aacgacgatg
      121 cgtttcggtt tgtttttgta gttgatgccg tagctgacag caccgaggcc catggggccg
      181 cagccgccga ggatgatgat gttgccgcct tctttggtgc ccannnnnnn nnnttattcg
      241 tgtggtagtt ggcgtgatag ccgccgatga tgcagctcat gggttcgcca agggaggctt
      301 cgaagaagct ttccccttcg taaggcatga gggcccctaa ttccatgact tcgtgaggga
      361 agatgcagta cgtggcatcg ccgccgaaga attcatagga gtaaccgggg gaagccaggc
      421 tgcctttgta gttcaaggcc ggctgctgag caaaacgcat gcccggtttg aactggtctt
      481 gccatttttt gccgactttt acgatgacac cggcaaattc atggcccgtg atgatcgggt
      541 tggtatcgat gttctgcgga gctcgtttgt ggttcttgcc ctgttcaacg agtttgtatg
      601 tcgacatgca gatgctgtcg gtgatgattt ttgctaaaat ttcgtcgtct ttaatttccg
      661 ggagttcaaa ttcttctaag cgtaaatcgc gggtaccgta cattctgact gcttttgttt
      721 tcatggtaaa atcctcctaa ataggtaagt agctgtaatt ttttatatga gtgacgaagc
      781 tgtttatgct tcgaggtttg caaaggtgtt gaagatgtcg tcggcagagc tgtggagcaa
      841 gtcttcgacg tcgtcttcat cggaacaggt gatggcgata ttgcttaaaa tatcgaggtg
      901 ttcgtcaccg acaccggcga tgccgatgac gagttttacc gtttcaccgc cccaatcagt
      961 gccgtctggg aaggtcatga cgactaatcc ggattttttg acttcgccct tgctggcttc
     1021 aatgccgtga ggaatggcga cggcgtttcc gatggctgta ttaaagactt cttctttatc
     1081 gagcatagct tgggtatatt tttcgctggt atagccgtcc tcagagaaaa tctttccgat
     1141 tttcttgatg atatcttctt tgggctgggg cgggcagttc aggatgatgt ttttcttctg
     1201 taaaatcgta ttcatagaga acgtctcctt ttatttttcg ttgcgggnnn nnnnnnncga
     1261 cgagcatgcc gtattcggga gctgccataa aattggtaat cgtaatcaga cgggcgttgg
     1321 gagcactggc tttagctcgg tcggcgagat ccttgtggca aacgacgagt tgggcgtcgg
     1381 ccggaacttc agaaaccgat gcgtggctga cggtgatgtc ggtcagtcct gcttttttga
     1441 attttttttg caggacagcg gcacccatgg cgctggagcc catgccggca tcgcaggcga
     1501 agacaacgtt ggtgatatgc tttaaatcga caggagctgc agacgttgtg gtcgctgcct
     1561 tcttcgattc ggctttcatg tcctgcatct tctgctgtgc ttcttcgagg gattcacccg
     1621 atttggcatt ggacaatttg atgatcggcg atgcgatgac gaaggacacg accgttgcga
     1681 tgacgatgct taagataacg gcaaaggttg aacctttggg agccatggcc aggtaggaaa
     1741 tgaggctgcc gggagccggc gggccggaaa ggccgaggtc aaagatcatg ttgtagaaca
     1801 gggctgcgac gctgccgccg atggtggcca ggagcagggc cgggttcatg aggatatagg
     1861 gaaaataaat ttcgtgaatg ccgccgaata cgtggatgat caaggcaccg ggagctgact
     1921 ggcgggtcat cttgtctttg gcaaataacc agtaggctaa gaggacgcct gcgccggggc
     1981 cggggttggt ttcaatcatg tagaagatgg atttgcccga ttcagcgacc tgttcagcac
     2041 cgaggggagt gaagatcccg tggttaatgg cgttgttgag gaacaagact tttgccggtt
     2101 ccacgaagag cccgatgagg gggaagatgc cggcctgcag taggaattgg acgcctgccg
     2161 ttaagaagga gaggatgacg cccatgaccg gtccaatcag ttcgaagccg atgatggcca
     2221 tgatcatgcc taagataccg acggagaagt tattgaccag catttcaaaa cccggtttaa
     2281 tgtggccgtc gacagcggcg tcaaactttt tgatgacaag gcctgccagc gggcccatga
     2341 ccatggcacc taagaacatg gtgtacgtcg taccggtgat gacgccgact gttgcgatgg
     2401 cgcccatgac ggcaccgcga aggccgccga cgatcttgcc gccttggaag gcgatgagta
     2461 agggcagtgc ataggtcagg aacggtccga ccattttagc taactgttca tttggcagcc
     2521 agccactggg aatgaagagt gccgttatga gaccccaggc gatgaaggcg ccgatgttcg
     2581 gcatgaccat actgctgagg aagcggccga aggattgtat ttttgctttc atagtaagcc
     2641 tccaatttgt aaccataatt ccattgacgg agcagaactc ggcactccgg actgctctct
     2701 tgatggttac agtatacctt ccgtacatac tgaaatcaat taaaagtaat ttaactttgt
     2761 ccacattcaa tgtgacaact atgaaggaat cagtgctggc ctggcgtaag ccgcagctgc
     2821 cgcccctttc aattggtggt ataattaaga aaaacgacag ggaggagatg agcggagggc
     2881 tctttcggtc gtaattatgg atcacgagcc gctcatggcg gcacaattaa aaaaatgcct
     2941 gttagcagtg gatccggaca taaccgtcgt cgctatttgc tatgaagggg aacaagcggc
     3001 agcctgcctt catgaatata aaccggatct cgtcttcttg acgattgaaa tgccccgcat
     3061 cggcggtctt gccattgcca aggccattca gggcatggaa ggagaccggc cggatatcgt
     3121 cttcgtcacc ggccagcgcg actttgtgtc ccatacgctg cggctgacgg ccctggacta
     3181 tttagtcaag cccgtaaccg aaggggaagt tgctcaggcc gtccgaaagt tcagggccct
     3241 gcacataaaa aaagaacctt ctccgagaga agattctttg gcggatacgt cggctgatga
     3301 cgggccgatt tctcttccga cgcggcggtt ttccgtcgat gagggagaga agataaagct
     3361 cattgcctgt gaggatattc gcttgattta tgccgaaaag cgccggactt ttttagtgac
     3421 cctggaaggg aaaacctatc ctactcattt cagcctgatt cagtttgagc agcggttgcc
     3481 ggcatcggta ttttttcgct gtcaccgcaa ttatatcgtc aatatcgatg aggttcggca
     3541 aatcgaacca tggttcaatc atcaatacgt attgattctt caagggcagg aaaaggaacc
     3601 gattccggtg ggccgctctt atataaaaaa actgaggcaa tatatttatt tataatccta
     3661 gggcctgagc aaaatagcct tttaaaatcc gctgcagatg gcaacggatt ttttcttcgt
     3721 catggccata aacggcctga gaaaaggcgg aatcactgnn nnnnnnnnat ggcactgctg
     3781 atggagccca ggacgtcggt gtgaagacgg ctctgttcgt cctggggcat gactaaaaac
     3841 agaatcgtcg agatgccgtg aaaatacggg ttagtaaagg tgtctttgtc aggaacggcc
     3901 gtatagaata aggcctgatt taccgccgtc gttcggcagt ggagcagggc gaagtcctgt
     3961 tccgggaaaa tctggctgtt cagccgttcg cgtttgatga tggcatcaaa cagttgggcc
     4021 gactgttctt catcttctac tgcctccgat gccagtgtgt ggacggcttc ggcaaaggta
     4081 acggctgccg gcagtgtata ttggcgataa gctcgcagga tatcggcgat tcctttcgac
     4141 agaagctgcg tcttatccag gaccgctacg aaatcgtctt cttccggttt atcgatgaaa
     4201 actcgttttt tggggacatg ggcatactct tcgattttgc cggtaatacg atgaagttcc
     4261 gacggcgaaa tgagggggga gacgaagagt acgtcggcgc cgggaaggga tatgggcagg
     4321 ctggaaacga agaagtccgt ctgttttaga acttcttccg ttacggaatc actgccgtac
     4381 gtctgaagct gtacgttttt aggcagttta ttttgaagac gggtcatcat cagtcgggca
     4441 atgccgaagc cgctggcaca gacgatgccg atggagacac tgcgcgtaaa ggctttgttt
     4501 cccttgattc gttctaaggc ggcaccgaag tgaatggtca gatagccgac ttcatcatcc
     4561 gatacggggg tatcgagaga cagggataag acgttagctg cccgctttgc tttttgaaag
     4621 acgtcggcat attcctcctt gatagcgtcg agtaagggat tgatgatatt taaatggtgc
     4681 tgaaggcgga tgatggtagg ctgtaagtgg acgaagaggc cttgaagaaa ctcgtcgtca
     4741 cagcgaaggt cgtaagctaa acgggtatcg aaggcttcaa tcatagcgtc gatgacgcgt
     4801 aagagttccg attcatcgac gctgctggga agatcccaaa gatcattgtc ttgataccgt
     4861 attttcgcgc ctttgatgtg aagcaggaga tacgaggctt caatgggcgg catcgtaatc
     4921 tgaaattccg ctttcatggc ggcgacgatt cgtcgtgcca gggcggcgtc ttcgctgtcc
     4981 tcaagaatgg gaacaacctc ttcgtccgta tcgtcaggct ggcccttggc aatgcgttcg
     5041 acggcgatgg cgatgtgaat gatcagtccg attttagccg tgtccgtaaa ttggtcgatc
     5101 tttttttcgt ctaatttcac gaggacgtca tagacgcgct gaatcgtatg ggtgttcagc
     5161 agctggtaga cgcccttatc ggtgacatgg gccaaggctt tcgataagga ttcgtcggcg
     5221 ttttgcaggt ccttgaaggc gtcggcactc gtcgtttcgc tgatgaagcg gtgcatggct
     5281 tcgcggtaat ctttttcgct gcctgtgagc aggacaccgt aaccctgttt tttcaggacc
     5341 tttaaattat ttttttcaag ccaaggggat aaggactcta aatctgaagc cgctgtcgct
     5401 tcactgacac cgagcagggt gctgtaatag agcagttttt tcggcattcg gtcgcgtaaa
     5461 agctcaaaga gcaggtaacg gcgccgcgta tctttatcgc tgaagtccat ggcggacgca
     5521 tcagaaaggg cttcggccag atttttctta tcaaggggcg acccgtctaa acggatgccg
     5581 gcccgttttt ggcggaccag tttcagctta tacgaggaca atgccttttc ggtcccttcc
     5641 agttcgcgtt ggaccgttcg cttgctgatg ccgagatcat cggccagggc ctgttcggag
     5701 aggtagtctt cctcttttaa taagcgtcgc agaatcgctt gcatgcgagg tgtaaaatcc
     5761 atgtgctcac ctcctgtggt ttcatttaaa tgcaggcagg atactccgac ctcttaggtc
     5821 ggggaggaat gcccgcttca cctcacttt
//
LOCUS       DAWDXZ010000046         5778 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_3986_length_5778_cov_11.021143, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000046 DAWDXZ010000000
VERSION     DAWDXZ010000046.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3986_length_5778_cov_11.021143"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    987..996
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1393 a         1498 c         1387 g         1490 t
ORIGIN      
        1 aggccgcatg gggatggtca gtacgtcgtc acagcattcc aaaaattcat cgggcaagcc
       61 gttcccttcg ttgccaaaag ccagaataac cggccggtcg taatcggctt cgacatagga
      121 tacggtccct tcggctgccg ttccatagat gttccggtcc tcatcgctgc agaattggtc
      181 gagctgttca tcggttacat cttggacgac aggcacttta aaaatcgccc ccatggcgct
      241 gcggaccgtc ttatcgttgt agacgtcgac acagccttca ctgagaagga cagctccgac
      301 gttagctgct gcagccgtcc tgaggatagt ccctaaatta cccggatcct ggacggagcg
      361 cagatagagg acggcctttt ccggagcaac ggccggcaag gcgtcaaagg tataggcaaa
      421 aaagggcagc atagcagcca gcccttgagg cgtcttggta tcctttaatt tatcgtatac
      481 gctgtcggta acggcataaa aagtccatcc taattcaagg cctctggcat agacttcgtc
      541 aaagccggca agttcccgtc ctttaggcga cacaaagcaa atggcatcgg gaatccccat
      601 aacagcggca tcaccgatga cccgcaggcc ttcgacaaag acgcgctgat gagcttgacg
      661 gccttttttc tgcttcaagg agcaggccat ctttacccac tggttatcgc gggacacaat
      721 ttcgttcata gtacctcctg tcatcaacat ggaaaaaaag gccctgccgc acgagcgaca
      781 ggacctcata agccatcact actgctgatc cggcgtatcc tgatgtccat tgttcttcag
      841 caggctttcg cgattctgct gaatgctgga taaggtcttt tccaatgttg attcaacgta
      901 tttgaggacg tcgccggcat aggtaatgga gctgtttttc agctgttccg acgagtcatt
      961 ggcagtttgg ataacctggt tggcacnnnn nnnnnncctg ccgtaccagt tcactttctt
     1021 cggtcaattt ggcgatgtag tctttcgcct gagctaccat gccgtcagcg tgatgctggg
     1081 catcggcaat gaccttgtct ctttcggcca atatcttttt ggactgttcc aattcagccg
     1141 gcatggattc tttcaaatcg tcgatgatct gaaggatttc ttcttcttct accatttttt
     1201 tgttagtaaa gggaactttt gaagcgttca ttactaagct ttccaaatct tctaacagtt
     1261 tatccgaatt catgtagtct ccactcctaa gcattgtact tgtctgcaaa cttcactttt
     1321 agtttctttt caacgtaatc gggaaccata tcatcgagct tgccgccaaa gcaggccagt
     1381 tcgcggatgc ccgtcgagct gatatatgaa tactgggtac tcgtcatgat gaataccgtt
     1441 tcgatgcgtg gctccacttt tttcatgagc agggcccgtt ggaattcata ttcaaaatca
     1501 ctcagggccc ggaggccgcg aataatgacg gtcgcaccgt gatcgatagc gtattcattg
     1561 agaaggccgt gaaagctgac gacttcaata ttgggaatat gccgcgtcgt ttctctcagc
     1621 atctcttctc gttcggccat cgtaaacaac ggcttcttta cgggattttc aaaaacagca
     1681 acgataagtt tatcgactag tttgctccct cgttcaaaaa tatcaatgtg tccattggta
     1741 acaggatcaa aactgcccgg acaaataccg attcgcactt ctttcaaact ccttcccggc
     1801 gacgacggat aaaagcaatc cgcgtgtcat ttccatagga tttttctctg atgacctcat
     1861 acaatgtgtt ctccgttatg ccttcattcc ggctgtattc catcagaatc aatccaccag
     1921 gagccaacaa atcgtattga cctatcaaaa caattgtatc gttcaccaaa tttttgttat
     1981 acggcgggtc ggcaaagata taatcaaact gccgcccttt cagccgtgcc aaagcttttg
     2041 ccgacgggag ccgaaggacc tcagcgcgtt catcaaagcc gcagagcgac gcattctgac
     2101 gaatgagggc ctgcgtataa tggtcgatga agacggcaga atcggcaccg cggctcaagg
     2161 cttctaatcc caaggcacct gtacccgcaa agatatcgag aacccttgcc ccggccagac
     2221 tgcctctgct ttcaagcata ttaaaaagac tttcccgcgt gcggtctaag gtcggccggg
     2281 tcagcattcc tttcggcgac ttcaaaattc gccctctggc actacccgat atgattctca
     2341 taagatcacc tgctcgtaca taatcaatta tacacgtttt ttagtacata tcaatctatt
     2401 ttttctgaat tcgttcgacc ttcagttctc gtccctgtat caagcggata atattatcct
     2461 tatgacgaac gacgacgaga atggcggcca ggaggccgaa aatcgtcagc ggcagcggct
     2521 cgccaaagac gtacatcaga atcggcacca aaatagcggc tacgatggag ccgagggaga
     2581 cgatgcgcgt aatggcaacg atgacgaccc agacggcaaa gacgatgagc gtttcccacg
     2641 gcgccaagaa caagatgatg ccaaggccgg tggcaacgcc tttaccacct ttgaatttca
     2701 gaaaaacaga gcacgaatga ccgacgatgg aaagcagccc gccggcaatc atgagataaa
     2761 tctgatcagc cggagtgccg atgccggcct gggaagcaag ctgccccagg tagacaccga
     2821 gcatgccttt cgcgacgtcg cagaggagga ccaaaaggcc gccgacagcg ccgaagaccc
     2881 ggtaggcatt ggtcgctccc gtattatggc tgccgtactc acgaggatcc gtatgataca
     2941 ggaccttgcc gatgataagg ccgctcggaa tggagcctgc gagataagcc agaaccaaga
     3001 agaggatacc ggtcatcata agcggtcacc atccttctta ccacgcagga taagccggat
     3061 gggagtgcct tcaaaaccaa aggtttcgcg cagtcggttt tccaaaaagc gcagatacga
     3121 aaaatgcatg agctggggct cattgacgaa aaggatgaag gtcggcggct ggaccgaagc
     3181 ctgggtcata taatagattt tcagcagtcg cccgccttta gccggcggcg gattggtaag
     3241 ctgggcatct tcgatgagtt catttaagac gctcgtcgat acgcgcatat gctgctgttc
     3301 cgatacgaat ttgaccatat cggccaggcg attgatacgc tgcttcgtaa gggccgaagc
     3361 aaagaggatg ggcgcgtact ggaggaaggc cagttctcga cggatgtctt cttcaaactt
     3421 atggctcgtc ttgctgtcct tttcgacgag gtcccacttg ttgacgacga tgatgcagcc
     3481 tttgcccgct tcgtggacgt agcctgcgat cttcgtatcc tgttcggtaa cgccttccgt
     3541 cgcatcgatg accaggacgg caatgtccga ccgatcgacg gcccgcagag cccggacgac
     3601 gctgtaccgt tcgacaggca tgtcgatttt ggccttgcgg cgcatgccgg ccgtatcgat
     3661 gaggacgaag tccgttcctt cataagacca atgtgtatcg atagagtcgc gggtcgtacc
     3721 gggcacgttg ctgacgatga cccgatcctg ccctaagagg gcattggtga gcgacgactt
     3781 gccgacgttc ggacggccga cgagggagat atgaatcgta tcgtcatctt cttcaagggc
     3841 cggtacttct ttgatattct tcaggacttc atcgaggagg tcgccgagat tcatcaagtt
     3901 gacggctgaa atcccgatgg ggtcacccaa gccgagatta taaaattcat agatgttcat
     3961 ttcctgttcg acactgtcga tcttattgac gaccaagatg accggtttcc cgcaggaacg
     4021 caggatatgg gcaatttctt catcctgagg ctggatcccg actttcccgt cgacgacgta
     4081 taagatgacg tcggcttcgt gaatggccag ttctgcctga tagcgcatgc tggtgaagat
     4141 gtggtcattg gaatcaacga attcaatgcc ccccgtatcg atcatggtaa attcccgacc
     4201 gagccattcg gcatcaaaat aaattctgtc gcgcgtaacg ccgggaatat cttcaacgat
     4261 ggaaattcgt ttcttgacaa tcgcgttaaa cagcgtcgat ttgccgacat tcggccgacc
     4321 gacgacggcg actaagggtt tatacatggc attatccttt ctataaacga aaaaaggata
     4381 accggtacag cccggctatc ctctttttac aaagccttag ttgttttctt cttcgttatc
     4441 ctggttagcg aggtagtcgc gaacttcctg ttcttcttca tctttctgaa tagccagaac
     4501 gctgagagaa atcttcttct tatctgtatc gatgccgatg atcttaacgt tcatgacatc
     4561 gccgatgtcg accaggtctt tgacagccac gccgcgtttt tcagttaatt cagaaatatg
     4621 gagcaagccc tgcagttccg gatcgatagc taagatagca ccgaaaggca agagtttctt
     4681 aacttcacct tttacgatat cgccgacttc aaatttttcg atagctgcaa tccaggggtt
     4741 cttctgaagt gctttcaagg agagggaaat acgattccgt tcctgatcga acttctgaac
     4801 aaggacttcg agttcttctc cggcctggag aacgtcttcg acagcattga tgcgcttcca
     4861 agaaatatca gaaatgtgaa gcaggccttc tacgccgccg agatcgatga atgcgccata
     4921 gggcatgatc ttaacgacag tacctttgat gacagcccct tcttcaatgt gtttgagggc
     4981 ttcttcgcgt tttgcttcgc gttctgcttc aagtacagcc ttacgggaca ggaccagacg
     5041 gtttttgtgt tcgtcaatgt cgatgacttt gacctggaag gtttcaccga cgaggtagtc
     5101 caaagacttc acgaatttga tatcgccctg agagaggggg ataaatccgc ggagagattt
     5161 gatagaaacg acaagaccag ccttgttcgt ttctttacca acacattcga cgagctgatc
     5221 attttcgaat gcttcgcgaa cttctttcca gtcttcgact ttcttcatct tgacgaggga
     5281 aacgacaata gctccttctt ctttgatgtt gttggcaatt tcaacggtca gttcatcgcc
     5341 gactttgaca aagtcacttg ctttttccgg agcgggaaca gccatttcct ttttcggcag
     5401 aattgcttcc tttcgtaccg atggaaacat aggcagcatc atcattcaca gaaataaccg
     5461 tgcctttgac cacgtcacct ttctttggat tttcttcata gtcatacttg tccaacaatt
     5521 cttcactcat tacttctgtg ccttcagctt gcaaattttc cataatctta acaacctcct
     5581 gtattatcca atccggcgta gaagcgccgg ctgtaatgcc tatatgattc ataccactga
     5641 accagtccgt atccaattcg gctgctgttt caatgtggta tgtcgggcaa tcctgttcac
     5701 ggcatacctg cgcgaggcgg ccggtgttgg cgctgttctt gccgccaatg acgatcatgg
     5761 cgtccacctg acctgcca
//
LOCUS       DAWDXZ010000047         5683 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4047_length_5683_cov_7.249467, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000047 DAWDXZ010000000
VERSION     DAWDXZ010000047.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5683)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4047_length_5683_cov_7.249467"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1036..1045
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3669..3678
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1245 a         1437 c         1596 g         1385 t
ORIGIN      
        1 aatccggcgc atcgtcacga ccaatccgaa aatcgtcgct gtcggtgaaa ccggtctcga
       61 ctatgaccgc atgttctcta aaaaagaaaa ccagctctac ttttttaaga aactgattgc
      121 cctggcagaa gaactggata aaccgatgtt tcttcatgaa cgcgatgccg gagacgattt
      181 tttagcctgt tttaaaggcc atgaaggggt ctgtccgaag tccgttgtcc actgctatac
      241 cggtgataaa gaaacactgg cccgtatgct cgatatgggc ttttatatcg gcattaccgg
      301 ctggatctgt gatgaacggc gagccgatgc actgcgcgag gccgtgtcca ttttgcccct
      361 ggatagggta cttctcgaga cggatgcgcc ttatctgacg cctcgcgggt ttcacttgcc
      421 acggacgaat gttcccaata atattaccta tgttgccagc accttggcta agtatatgga
      481 tgtttctgtc gaagccttga cggttcaggc aaaggccaat acggagcgac tgtttcgctt
      541 ataagggttt tgttaatttc tccccgatta aggaggtctt tttatgaata ttgtgcagta
      601 tacgaaggcg gcgtccctcg aagaagcgtg ggaattgaat caaggccgcg gggccgttgt
      661 cctcggcgga tgctgctggc tccgcctgtc gccccaacgg cgcattgctc aggccatcga
      721 cttgtcaggt cttggcctcg atgcgataga agaacaggat gactctttta aaatcggagc
      781 catggtcact ctcggagcct ttggcagtca tgaaggactg ttgacctata cccatggagc
      841 tgtaaaagaa gcgacacgcc atatcgtagg gactcagttc cgcaacttgg cgaccattgg
      901 cggcagtatc agcggccgct tcggcttttc cgatatttgg accctgctct tggcgcttga
      961 tgccgacgtt gaattgtata agggcggacg cgttccgctg tctgattttg gaaagaccgg
     1021 gcccgggaag gatatnnnnn nnnnnatcgg tgcccgtccg ggacgagccg aggtcatgac
     1081 actccctgtg gatacggtta aggcccttgg ctgtgaaaag gccggtcggg aatttgccga
     1141 aaaagtgacg tatgaaacta accaacgcgg ttcttccgaa tatcgccatg atatggctgt
     1201 cgtcttatgc cggcgcctgt tggctcaaac gatgggagat gatgtctgat gatgattacc
     1261 tattggctta atggaaaaaa atcctcggcc gatatcgagc cggggatgat gctttatgat
     1321 tttctccgca gccggggatg ttatagtgta aaatgcggct gtgaaacgac aaactgcggc
     1381 ctctgtaccg tttgggtcaa tgatgtgccg gtcttgtcct gcgccgtact agctgtccgc
     1441 atcgatggct gtcaagttac gacccttgaa ggcctccaag aggaagccaa ggaatttgcc
     1501 tactatatgg gacttcaagg tgccgaccag tgcggcttct gcaacccggg cttcgtcatg
     1561 aacgtcctgg ccatggctcg ccgcttcgat tcgccgacgg aagacgaaat caaggaattt
     1621 ttatcgggta acctctgccg ctgtacggga tatatgagtc atatgagggc tattaaagag
     1681 tattttaagg ccaaggggaa ggaggtccgg tcatgatgga atttaaatcg atcaataagg
     1741 tcgtagccaa gaaggattcc caagccttgg ttacggggca gcctgtctat acggaagaca
     1801 tcatgcccaa agactgcctc atcgtaaagg tgctgcggag cccccatgcc agagccatcg
     1861 ttaaaaaggt caataagagt gctgctgaga aaattccggg catcgtttgt atcgtaacgg
     1921 ccgatgacgt acctaagtcg cgatttacca ttgccggcca gacctatccg gaaccgagtc
     1981 cttacgaccg gcttattttg gataaacaga tccgttttgt cggcgatgcc gtagccctcg
     2041 tggcagggac gaatcaagcg gcagtcgata aggcgctgcg ggccatccgc gtcgaatatg
     2101 aagtccagga accgctcctc gactttacga aggccttgga caatgatatc gtcgtccacc
     2161 ccgaagacga ttggaagccg ttagtcgacg tcggcggcga caaccagcgc aacctcgtcg
     2221 ctaaagcctg cgaccgtcac ggcgatatcg atgccgtcct ggcttcgtca gacgtcgtca
     2281 tcgaccggac gtatcacact cgtcagaatc agcaggcagc catggaaacc ttccgagcct
     2341 atgcttataa agacgcattc ggacggctga ccatcgtcag ctcgacgcag gtacctttcc
     2401 atgtgcgccg cgtcgtttcc catgcccttg aaatccccaa atccaaggtt cgggtcatca
     2461 aaccgcgtat cggcggaggc ttcggttcga aacagaccgt cgtcatggaa gtctatccgg
     2521 ctttcattgc ctggctgaca ggcaaaccgg cctatatggt ctacagtcgg gaagaatcgc
     2581 tgaccgtatc gacgccgcgt catgaatcgc agatccgcgt cgttctcgga gcgtcgaaag
     2641 acggccatat ccggggcatt ctcctcgatt ctttatggaa cgccggcgcg tatggtgaac
     2701 atacgccgac gacggttact ttatcgggac acaagtcgat tcctttgtat aatgccgctg
     2761 aagcctttca attcagctat acctgtgtct ataccaatac catcgctgcc ggggcttacc
     2821 gcggctatgg ggcgacacaa ggcctctttg cgctggagac agccgtcaac gaattggccg
     2881 aagcccttca tatgagtccg gtggaactgc ggtttaagaa tatggtccgc caaaatcagg
     2941 tcctgccggc ctattttaat gaaacggccc tcagctgtac cctcgataaa tgtctggaac
     3001 gcgtcgtttc catgtccggt tgggacgatg cgtcgccgcg aaaagtcctg cccaacggtc
     3061 atatccaatc cgtcggtttg gctatcgcta tgcaaggttc cagtgtctcc aacgtcgacg
     3121 tggcctcggt tacgatcaaa gtcaatgatg acggcttcta tacgctccac gttggggcta
     3181 ccgatatggg aaccggttgt gataccatcc tcgcccagat tgctgccgaa tgcctcatgt
     3241 gttctgtcga cgacatcgtc actgacggcg tcgataccga tacgtctccc tatgacagcg
     3301 gctcttatgc ctcgtctacg acgtatctga caggcaatgc cgtcgtcaag acctgtgaaa
     3361 gcttgattca tcggatgaaa gaattagcgg ccattaaatt ggggctgtcc gatacggatt
     3421 tggatgacct ggaatttgac ggcaaggcca ttacctgcct tcagaccggt aagtcgatta
     3481 ccgtccgcga tctcggcaac ggggccatgt tcatgaacgg cattgccctc gaggcgacgg
     3541 aatcgtgcta ttcgccgacc agtccgccgc cgtatatggc cggtgctgcg acgatcgatg
     3601 tcgatcccga aacggggaac atcactctct tgaaatacga tgccgtcatt gactgcggta
     3661 cggtcatcnn nnnnnnnngg cccgcgtcca ggccgaagga ggcatcgtcc aggctatcgg
     3721 catggctctt acggaaaacg ttcagtatac gccgacggga cggctgcaga ataattcttt
     3781 catgcagtat cgcctgccga cgcgtctcga cgtcggtccc gtcaacgtcg aatttgaacc
     3841 gagctatgaa ccgaacggtc ctttcggcgc taagtccatc ggtgaactgg tcatcgactc
     3901 gccgtccccg gccattgctc acgccgttta taatgcaacc ggcgtatggg tccgcgacct
     3961 tcccattacg gctgaaaaac tctatcaggc tatgaaacat aagacggtct gagttatata
     4021 ttctgaaaga caacgaaacg ccattccatg acggtgtatc atggaatggc gttttttgtg
     4081 atggcgttat ttatttttca agggaactgt tgatgcggac gccgctcatg aagggaagga
     4141 ccggtttggt gtcttcctgg actgtttccc ggatgccttc aaaagtcgtg gtcagcatta
     4201 acttcagacg gttcgtctgg ttgaccgagc tggcactggg gtcgtagtcg atggcgacga
     4261 tattcgtttc agggaaggcc gctttcaagg tcttgatcat gccctttccc gtgacgtggt
     4321 tgggcaggca ggcccagggc tggaggcaga cgatgttctt ggccccgcga tggatgaggt
     4381 cgatcatatc ccccgtgagg aaccagcctt caccggcttc atggccgagg gacaagaatt
     4441 tcttggcttc gtcggcaatg tcataaatcg acgtaatggg atcgaagtgg cggctcttgg
     4501 caacggcctg ttcgtatggt ttgcggtacc attcgaggag gcggacgccg gtcttgctga
     4561 ataaggcgtc tttccagctt ccggaaaggt acttatggcg gaactggtcg tcgagcagac
     4621 cgtagaggaa gaagtcggcc aggccggaga cgatgatttc accgccttct tcttcaatca
     4681 tgcggacgat gtggttgttg gcaatggggt ggaacttgac gtagatttcg ccgactaagc
     4741 caatgcgcgg cttcttttcg tcactcattt cgatattgtc gaattcattg atgaggtcgc
     4801 gaaggttgcg cttgaaggat ttaaagtcgg ctttcttgag ctgctgtgtg catttttcct
     4861 gccacttatg gaacaaggct tcggccgtac ccttgacctt ttcataaggc cgcgtgcggt
     4921 acaagacttg ctgcagggcg tcgccgtaaa tcatacccat gatgaggcgg tgccagaagc
     4981 cgagccccgg ctggaaaccg ggctgcttgt tcatgccctt catattgagg gataaaatgg
     5041 caacctggga catattggca tcgttcaaag cttttcgaat catgccgatg taattggtgg
     5101 ccctgcagca gccgccggtc tgggaaatca tgaccgtcgt atggtttaaa tcgtattttc
     5161 ccgactttaa ggccgtgagg atttggccta aactcatgat agccggatag caagcgtcat
     5221 tgtggatata ggccagaccg gtatcgatgg cttcctggtt ctggtggggc aggagttcga
     5281 cgtgatagcc ttcgtgttcg aaggctgaca tgagcatggg aaaatggatc ggtgtcatag
     5341 acggggccag gatgacgttc tttttggcgt cttctttggt gaagatcgtc ttttcatagg
     5401 aatacggttc gagggtgtaa tcttctttcg gccgatgatt catgacgaac agcaaggagc
     5461 gcaggcggat gcggatggca ccgaggttcg agccttcgtc gattttcagc agggtgtgga
     5521 tcttgtggcc gtgggtcagg atatcctgta cctggtcggc gacgatggcg tcgaggccgc
     5581 agccgaagga attgagctct accagttcga ggttcggatg ctgggtaacg tactgggctg
     5641 cccgatagag gcgggagtgg tacgaccact ggtcgacgac gcg
//
LOCUS       DAWDXZ010000048         5666 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4064_length_5666_cov_8.938870, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000048 DAWDXZ010000000
VERSION     DAWDXZ010000048.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5666)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4064_length_5666_cov_8.938870"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1375 a         1345 c         1547 g         1399 t
ORIGIN      
        1 ccggcggtac cgtttactta tgcagtgccg acggtgacgg caatatggtg tctttcatcc
       61 aaagcaatta caatggcttc ggttcgggca tcgtcattcc gggtcgtggt atttctctca
      121 atgacagagg gcataatttc tccatggaca cagccagtga caactgtctc ggcccgagaa
      181 aaaaatcgta ccacacgatc attcccggtt tcttgacgaa agatggggag gctgtcggtc
      241 ctttcggtgt catgggagcc tttatgcagc cccagggaca gctgcaagtc ttgctgaata
      301 ccattgacta tcaccttaac ccacaggcgg cccttgatgc gcctcgttgg cagtgggtcg
      361 gcggcaaaac gattgaagtc gagccggaca tgccgcagga tgtcatcgac ggactgcggg
      421 cacggggcca tgatatcatc gtcaaaggcg atattcagac ctatggccgg gggcagatca
      481 tttggcgcag tgaagcgggc gtgctggtcg ggggaacgga gcctcgggcg gatgggaccg
      541 tggctgcctg gtaacgggcc tttcttttct tctcgtctaa aatatgatag gatggagaaa
      601 acaaaaggag gtattattcc ccaatgaaac cgatgaaacc aaaactttcc ttgttagaca
      661 cggcaccgac gccgctccag tatttatccg gcttgtcaca ggagttattt cgcgaaatat
      721 acattaaacg cgatgattta acgccctttg gcggcggcgg taacaagctg cgcaaattag
      781 agtatttgat gattgaagct ttgaatgctc aggccacgac catcgttacc gtcggagcac
      841 cccagaccaa ccacggccgg ttgacagcgg ctatagcggc caaattcggt ctgcactgca
      901 tcatcgttgc cgtcggagaa cccgaggcgg aattgagcgg caacttgttc ttagacggta
      961 tctttggcgc ccgtgtcgtc ttaaagcgag atgacgggcg agaccacgac gtacagctcg
     1021 aagaaacggt caagaaggtc atcgaagctg aagtaaagaa aggtgaacaa gtttacttca
     1081 ttcccatggg cggcagcgat acgacgggcc ttctcggtta tatggactgc gcgcgtgaac
     1141 tcgatgctca ggcaaaggac cagcaaatca gcggggctac ggtctacgtt cccgtcggca
     1201 gtatggggac ctatcttgga ctgtactgcg ggcttaaatc gatcgaatct gatttaaaac
     1261 tgatcggcat tgccgtcatg ccctttgata tggaaaaatt aaaggcctac tttacgcagg
     1321 ccaaagaaga atacggattt aattttgagg gtgacttcca cgtcgaaacg ggatatatcg
     1381 gaaaaggtta caatgaaccg gaaccgagaa ttcgggaagc catttacacc atggcccgcc
     1441 atgaaggaat tttgctcgat ccctgctata cggggaagat gtttgccggt gtcctgtcga
     1501 tgattaagga aaagaaaatc aagttgggac gccaagtcat cttgctccat accggcggta
     1561 tgcccggcct ttatgcagct cctcatcggg aaaaaatcga acgggacgtg aaagatcaga
     1621 tccaagtcat aaaataagga caagaaagct cttagaaaaa caggtgctgc aggatgaagt
     1681 aggataaggc acccatgaca gctgtgcagg ggatggtcat gacccaggcc gtgaccatct
     1741 gacgggcgac gctccagtgg acggctttaa ctcgctgggc cgacccgacg cccatgatgg
     1801 agccggaaac gacgtgcgtc gtcgatacgg gcaggtgcag caaggttgct gaaaagatga
     1861 cgacagctga gtttaagtca gctgcaaagc cgtggacggg atgcatttta aaaatcttgc
     1921 cgccgacggt gcggatgatt ttccaaccgc cgacactcgt ccccaaggcc atggccaggg
     1981 cgcagaggaa ttttaccagg tcagggactt ccaagctggt gataaaaccg ccggatacca
     2041 gggccagggt gatgataccc atcgatttct gggcgtcgtt ggaaccgtga gaaaaggcca
     2101 tcagggccgc tgatacgact tgcagccggt tggcaaccag gtttactttg gccggtttaa
     2161 aggaatggca gaccatgaag atgatcttca tgatgatgaa gcccgtaatg atagccgaga
     2221 taggcgagat gatgagggat aagacgattt ttaagatccc gaagccgttg agggccgctg
     2281 ctccggcaga aaccatgatg gcaccgatca tgcctccgat gagggcgtga gacgagctgg
     2341 acggcatgcc gacccaccaa gtcaggaggt tccagataat agcgcctacg aggccggcaa
     2401 tgatgatcat ttcattgatc aaggtcggcg aaacgacgat atctccgcca atcgtcttgg
     2461 cgacgcccgt cgaaatcatg gcgccgagga agtttaaaat cgcagtgccg atgatggcga
     2521 caccgggccg aatggctcgc gtcgatacgc aggtggcaat ggcgttggcc gtatcgtgga
     2581 aaccattgat aaagtcaaaa acaagggcca ggatgacgac catgatgatg agcgacgtat
     2641 caagcatact tcatggtcac ctctttaacg gtatttgcca gggtttcgac gttgtccgaa
     2701 gcttcttcca gcgtccccag gatgtctttc cagcggatga cgttgagcag gtctttttct
     2761 tcaccgctga acaggcggga aatttcctgt ctgtaaagat ggtcgatttc cgattccagt
     2821 cggtttaagc gttcggcatg gcgcatgagg ttagcttggt tgcggtcgat gtcttttaag
     2881 agggagaaaa tcttattcat ttcttccccc atgaggacca gctgctcggt catggtaacg
     2941 gcggcgtccg gagctgtggt aacgtggtac atgctggtac gcattaaagc tccctgaaga
     3001 gagtcgatgc tgttttcgag ctggcaggtc aatttataga aatcttcgcg gtcgattggc
     3061 gtgatgaaaa cgcgggacaa cttgaagatg atatcttgat tggtttttga tgattcatgt
     3121 tctaatttca cgatttcttt taaatgaagt gaaatcgtgg aaacgtcttg cataacttca
     3181 tgagcgatga cagcgccgcg atggaaattt tgggcattgg ctacgagata gtcaaagaat
     3241 tcttcgtgct tattgctgat actgaacata gttattcctc ctatacagac atgaaaaaca
     3301 ttatataaag gatatatcag tattttgcaa agaatatgta aagaataggt aaaatataat
     3361 gatgcgcttt gatttttttg cggcgatggt ataatggagt cattgctatt attttataac
     3421 ggtactgcca tatacggtcg tggaatacgg ctcgcgatgg tgctgattta ttctgaacat
     3481 gaggtgactt ttacaaatga atgaagaacg aaccgcacag attcatcccg tcgacgaaat
     3541 gctgccgact ggccaactgt ttgcttatgg gctgcagcac gtcctggcga tgtatgccgg
     3601 tgccgttgcc gtacctatca ttgtagccca ggctttgcac ctgtcgccga tggaattggt
     3661 ccacctgatc aatgctgatt tgttttcctg cgggattgcg accttgatcc agacactggg
     3721 tttttggaaa gtcggggcgc gcattcctat gattcaaggc gttacctttg cctcggtcac
     3781 gccgatgatc cttatcgggg ctgaacacgg cattacggcg atttatgggg ccatcatcat
     3841 tgccggttcg tttactttct tgattgctcc gttttttagc cgattcattc gatttttccc
     3901 gcccgttgtt acggggacta tcattacgat tattgggatt acccttctgc ctgtcgctgt
     3961 aaactggatg ggcggtggga atccaaaggc acctgatttt gcctcgccga tgaatttatt
     4021 cctcgctttc attactttgg ttattatcat tgccatttat cgcggtggca aaggcttttt
     4081 gagcaacgta tccgtcttgt taggccttat cggaggtacg attattgcca tggcgttagg
     4141 gcagactgat ttttcggaag tcggaaacgc ttcctggttt ggtttggtta cgcccttttc
     4201 ctttggcctt ccgacctttg acatcggctc catcttatcg atgatggtcg tcatgatcgt
     4261 cactatggta gaaacgacgg gcgactgcat cgctatcggg tcgatcgtag gcactcctat
     4321 cggacgtgac cgtttgtccc gctgccttcg ggctgacggc ttgtcgacgg tcatcggcgg
     4381 tatcttgaac agcttcccgt atacggcctt tgcccaaaac gtcggtttga ttgccgttac
     4441 ccgcgtcaag agccgcttcg tcgtcgccgc ttccggcatc attctcattg ccttaggtct
     4501 ctttccgaag atggccgccg tcgtagcatc gattccgaat tccgtcttag gcggcgccgg
     4561 cgttgccatg ttcggcatgg tcattgccag cggcatccga gcactgagca aggtcgagtt
     4621 tgacggcacc tacaacctga tgattgttgc cgtcagtatc ggcgtcagca tgattaccct
     4681 gacggcgccg aatttcttcc acagcttccc gtcgtgggcc aatatcgttc tccacagcgg
     4741 gattactttg gggagtatca cggccgtcct gctcaatttg atcttgaatg gctgcggcag
     4801 ggaagaggat atggatggca attaaacaac tgtaaatgaa agtaaattcg ttttacctga
     4861 aagcgaggga tttttttgct gaagaaaatg gtggccttaa cctgtgccgt tgctttgagt
     4921 acaggcgcgg cctgggtaca tgcggaagaa ctcaaaacgg caccgcctcc ttttgcggtg
     4981 aaggttgaaa taaaaccggc ggctgcacca cagacttcaa atacgtcttc gcagaagacg
     5041 gtgacccagg ctgcttccaa ggctcaaccg aaggccgaaa aggctgttaa aaaatcgacg
     5101 gagaaaaaaa tcggcgtcaa tatagccagc cgtatactga ccctctatga aggcgataca
     5161 aaaatcaaaa tgtatcctgt cggggtaggc cggacgtcga caccgacgcc gacgggtttt
     5221 tataaggtac aaaataaaga ggtgaatccg acctggatcg atcctgacga tacgagcatc
     5281 caaatcgcat ccggtccgga taacccgatt ggctatcgct ggattgggtt tcatggcaat
     5341 tacggaatcc acgggacgaa caatcccggt tctatcggag gctacgtctc gaatggctgc
     5401 atccgtatga aggagcccga cgtggaagac ctgtatcaat acgtatcgat cgggacgccg
     5461 gttacggtat attatgaccg cctggtcatc gatgtcagtc cggaacacgc cgtatcctac
     5521 tacgtctacc cggacggcta cggctggcag gccttaagtg tagcgcaggt aaagaaagcc
     5581 ctggccggct atggcgtaga agactttgct gaattcaaag agatttcgga taaaataaac
     5641 cgttctgacg gcaatgtgac ctacgt
//
LOCUS       DAWDXZ010000049         5621 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4090_length_5621_cov_7.554977, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000049 DAWDXZ010000000
VERSION     DAWDXZ010000049.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4090_length_5621_cov_7.554977"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    939..987
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3791..3800
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4893..4902
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1433 a         1248 c         1478 g         1393 t
ORIGIN      
        1 ctgtgacgaa gttattaagg acgacagttt ttgtctggga agcgggtgat ggtatgaaga
       61 atagaacata ttcagaggaa gagtatttac agattgcaga aattcaacac tttgccttct
      121 gccctcgcca gtgggccttg gattatttgg agggacagtg gcaggaaaat gtcttgacca
      181 ttgaaggtca tgccctgcac gataaggccc atgaccctat gagtcgggaa aagcgaaagg
      241 ggaagatcat cgtacggggg atgcgcgttt tttcagcgtc tttggggatt tccggcgatt
      301 gtgatatcgt ggaatttcat caaaagaagg atggtgtctt tattccttct tttgagggaa
      361 cctacgaggt gatacccatt gaatacaagc ggggccggcc gaaggaagga gaggaagatg
      421 tctttcaatt agccttgcag gccctctgct tggaagacat gttttgtatg accattccct
      481 acggctacat ctattacggg gaaacgaggc ggcgggaaaa ggcaatattt tcagaggagc
      541 agcgggaaag aatcatgaag ctcttgggac agatgcgcca atatatccaa aggggatata
      601 cgccgaaagt acggcgccgc aagggatgcc ggaagtgttc cttagctgat gtttgcctgc
      661 cgaaattaaa gcaagatact tcggttcaag attatttaga aaagaagttg atcgaatgaa
      721 gcctttattg aataccttat ttgtaacgac ggacggggtc tacctgtctt tagatggaga
      781 aaatatcgtc gtcttaaaag atcaggccgc actggctcga tttcctctcc ataattttga
      841 gcaaattacg accttcagct atttgggagc cagcccggct ctgatgaaaa aatgcgccga
      901 tatgaatatt gccctgaatt ttatgtcgcc tcaggggcnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnagc tttataacgg ccgctgggta ttggaacgga
     1021 tgatgcgcga tcatgcgatg cggctgccca tggaggcgtt ccaaaaaacg tcaaccctta
     1081 tgaaagaggc ttgtcagtcc ctgatgtcgt ctgccgactt aggcgaaata cggggaattg
     1141 aagggcaggc cggagcggct tacttttccg ttttcgatgc gatgatactg aatcagaaag
     1201 acgacttttt ctttacggag cgaaatagaa ggccgcctct cgaccgaatg aactgcttac
     1261 tgtcctttct atacgccatg ctggccaatg atgccggagc agccttagaa ggtgtcggcc
     1321 ttgattctta cgtgggattc ttgcatcgtg accggccggg acgccagtcc ctggccttgg
     1381 atttgatgga agagcttcga ccggtcatgg tcgaccgcac ggctctgtcc atggtcaata
     1441 ccaagaaaat caagggcagt gacttttatg aggaagaagg gggtgccgtc ttgttgactg
     1501 acgaaggacg ccgaaaagtc ttgtcgtact ggcacgatca caaagaagcg acgattcagc
     1561 atcccttctt acaggaaaag atgaagtggg gcctcgttcc tcatgctcag gccttgttgt
     1621 tggcacggta tctgcgcggt gatttagacg gatacccgcc gtttatgtgg aagtagggat
     1681 gatcatgtat gtcttaatca cttacgatat cagtaccgtt gacagtgccg ggaaaagccg
     1741 cttgagaaag gtagccaagg tctgtcagaa ttacggacag cgcgtccaaa actccgtctt
     1801 tgaatgcaaa gtcgatccgg cccagtgtaa aaccttagaa ctgaagttgg agaacatcat
     1861 tgacgaagct acagacagcc tgaggtttta ttacttaggc aagaagaaag aactgaaagt
     1921 caaacacgtc ggggcaaagc cggcttacga tatcgagagc actctcatca tatgaagtgc
     1981 gcaagggaag ttcccttatt tttctcctgt ttgtggtttt tagcaacggt gaaaagataa
     2041 atggggaatc attgtcgtgt gatttagcct gattgtggag atgtgaccct atttttacga
     2101 ctgtttattg gggaaaaccg ctttgctgcc gcccctcctc atgggcggga tagacgccgg
     2161 ctgtattaat cggaagagag ttaccgattg gcggtgattc cgtaatgagt tttttttaag
     2221 ttaacggcct atggcgattg aaagggcggg ggcgtgaatg gtataatagg tgtgtaagtg
     2281 tcacacactt ttgatttgta tcgattaaaa gtgtgtgtgg attgaaagat atatgttgtg
     2341 gttagcttac cacaaacgat atcgcaccct ttttgggtgc gcggattaaa agtgtgatgc
     2401 ggcaagtggt tctttgaaac tgcttgccgc atcttttatg cagacacatt tcggaaatta
     2461 cggcagcaag atgagaaagc tcgcagctgt ttgatctttt ctgttatata tcgaaatcct
     2521 gagtgggtac atattgggca aaggccttga aaatatggta tacttaatga gcataagtga
     2581 ggagtgatct tttgaaaggt attgtattgg ctggcggaag cggaactcgc ctgtatccgc
     2641 tgacgacagt gacatcgaag cagttattgc cggtctacga caagcccatg atttattatc
     2701 ccttgtcgac cttgatgttg gccggaattc gggatatttt gattatttct acgccgacgg
     2761 atttgccgaa ctttgagcgc ctcctcggcg acggctctca ttacgggatc catttgagct
     2821 ataaggttca gccttcgcct gacggcttgg ctcaggcctt tatcctcggg gaagaattta
     2881 tcgatggaga accgtgtgcc atgattctcg gcgacaatat tttctacggt gccggcctga
     2941 cccgtcatct gcagcaagcc gctaagatgc aggaaggggc caccgttttt ggttactacg
     3001 ttgacgatcc ggaacgcttt ggcgtcattg aattcgacgg tgagggaaag gctatttcta
     3061 tcgaagaaaa gccgcagcag ccgaagtcga actacgccgt aacgggcctt tatttctacg
     3121 accgccacgt ctgcgaatat gccaaacagg tgaagccctc ggctcgcggc gaactggaaa
     3181 tcaccgactt gaataaaatc tatttggaaa aaggaaaact cgatgtcgta accctcggac
     3241 gtggttacgc ctggctcgat acgggaaccg tcgacagcct gagtgaagcc tcttctttcg
     3301 taaaggccgt ggaaaaccga gcggggatcc aaatcgctgc cttggaagac attgcttacg
     3361 gtcacggatg gatcaccaag gaagttctcg aagagagcgc tgaaaaatac ggcaagagcc
     3421 cttatggaca gtatttgaag aaagtcgctg aaggacggtt cctgcgggac gaataataca
     3481 ctacaggaag gccttatcgg aaggttacat cgagaaatgg gaggaaattt ttcatgaata
     3541 tcatcgtcac cggcggtgct ggatttattg gctctaattt tgtcttttat atgttaaaaa
     3601 cacatcctga agatcggatt atttgcttgg ataaattgac ctatgccggc aacttgtcga
     3661 cgctgcagcc ggtcatggat cagccgaatt ttcgcttcgt aaaagccgat atttgtgacc
     3721 gaaaagctgt ttatcagctc tttgaagaag aaaaaccgga catggtcgtc aactttgcgg
     3781 cagaatccca nnnnnnnnnn tgtcgatcgc tctattgaaa atccttcgat tttcttagaa
     3841 acgaatatca tggggacggc ggttctcatg gatgcttgcc gcaagtacgg catcgaacgc
     3901 tatcaccagg tatcgacgga cgaagtatac ggtgatttgc ctttagatcg tccggacctg
     3961 ttctttacgg aaacgacacc tattcacacg tcgagtccgt acagctcgtc gaaggcttcg
     4021 gccgatttgt tggttctggc ttatcatcgg acctatggac ttccggtgac ggtgagccgc
     4081 tgctccaata actacggtcc ctatcatttt ccggaaaaat tgattcccct catgatcatc
     4141 aatgctttga acgacaaagc tcttccggtc tacggcgacg gccttaatgt ccgcgattgg
     4201 ctctacgttg aagatcactg ccgggctatc gatctgatcc ttcgcaaggc ccgtgtcggc
     4261 gaagtctata acgtcggcgg ccacaatgaa atgaagaaca tcgacatcgt taccttgatc
     4321 tgccgggaac tggggaagga cgaaagtctg attcagcatg tgaccgaccg caagggtcac
     4381 gaccgccgct acgccatcga cccgacaaag attcacgagg aactgggctg gctgccggaa
     4441 acgaagtttg ccgacggcat caaaaagacc atcgcctggt acctggaaaa caaggattgg
     4501 tggcagccta tcgtctccgg tgaatatcag acctattatc aagagatgta cggccatcgc
     4561 ggttaaaccc gatgaaggga aagagggaca tactatgaaa attttcgtga ccggtgtcaa
     4621 tggacaactg ggacatgacg taatgaatga attggccgat cgcggtcata ttggtatcgg
     4681 cagcgatttg gccccgacat acagcggtat tgctgatgac tcggctgtca ccaaggcgcc
     4741 gtaccgaccc ttggatatta ccgataaaga agccgtatca caggctctac gtgaaatccg
     4801 gcctgacgcc gtgattcact gcgccgcttg gacggccgtc gatatggctg aagacgacgg
     4861 actcgtcgat aaggttcgag ccgtcaatgc cgnnnnnnnn nntgttaaga ccatgctcag
     4921 tgtcggcaag acccacgata cggtccgtgt cgtcaacgac caaatcggga cgccgaccta
     4981 tacctttgat ttggcgagac tcctcgtcga tatgattgaa accgataaat acggctatta
     5041 ccatgcgacg aatgaggggg gctacatttc ctggtacgac tttacctgcg aaatctaccg
     5101 ccaagccggc tatacgacca aggtaattcc cgttacgaca gcggaatacg gcttatcgaa
     5161 ggcaaagcgt cctttcaatt cgcgcctgga cagacagaaa ctcgtcgaag ccggctttac
     5221 gccccttccc gattggaagg atgccttgaa gcgctattta cagtaacggt ctgacacggg
     5281 ccttacaaga agataaagga gatataaatc aatggggcag attacagtag aaaagaatgt
     5341 cggaaatatc gaaggcctct gcatcatcac accggccgtt cacggcgatg accgcggttt
     5401 ttttatggag acctataatc agcgggatat ggaagaagcc ggttttcata ttaatttcgt
     5461 ccaagacaat cagtctatgt cgactaaagg cgtcttgcga ggccttcact ttcaaaaaca
     5521 cttcccccag tgcaaattag ttcgcgccgt tcggggcagc gtctttgatg ttgccgtcga
     5581 ccttcgccgc caatcacaga cctatggaaa gtggtacggt g
//
LOCUS       DAWDXZ010000050         5619 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4091_length_5619_cov_8.475557, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000050 DAWDXZ010000000
VERSION     DAWDXZ010000050.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4091_length_5619_cov_8.475557"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    219..241
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5389..5398
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1472 a         1345 c         1481 g         1288 t
ORIGIN      
        1 gggcggtgcc gatacgctgc gcggctatga agacgatgaa ttccgcggca acaaaatgta
       61 tgaagcgacc attgaatatc gttaccccat tgctaaaaaa atccagggcg tcgtctttac
      121 cgacgtgggt aatgcctggg gcggcgtaga aaacattcct tggtataaag aaaataataa
      181 gattcactat tccggcggta tcggcttccg tattacgann nnnnnnnnnn nnnnnnnnnn
      241 ntggtccaga gacggattgc tgccagtatt cagactgtcg gcaatcatgt atttctcaat
      301 caggacagcg aagacattgc cggccgctct gaggaatacg agcgaatcgt caacgatatc
      361 atcaatcgcg tcctcatcgg ctataccgta gagtctatcg ccatcaaccc ggggagccgg
      421 accgacctcg atgtccgtat ccggccttgg ggcgatacga tccgcaaggt gaacctgacc
      481 gtcgattacg gggctttgcc ggaaatggga cgccagatgg ttgataaaga cctcagcggt
      541 gccggtgaca tggtggaaaa cctgctcatc ggtctgcccg tcgatgccct cgattgggcc
      601 aatggtgccg tcaaatcggt catggaaacg gaattggaaa cgagagttcc cgagttttat
      661 ccgcatatcg tgattcgcgg cggtcaggaa acggacgtga cggtctatat gatgcctaaa
      721 cttccggtcg ttcgcaacgt tcgcgtagcc attaaaggag aagatgtgcc taaggtcatc
      781 tttctcagcg ctcggaacaa tctggagcat acctatgccg gtttggaagg gttgccggtc
      841 gctttcgtcc gtcgtcatga accggacgtt atggcggatc tttccggtga tgtgaagcgc
      901 caatgggtcg tcaagcacta cgggctccag gtgacaccga ccctttctgt cgatgaaaat
      961 acgacgatct atgtcacacc gaaaacggat ttttatgaca tccgcggcgg tgcctatctc
     1021 gatgtgggcc gcaggaacag tgatgacgat gataccgttc tcatggccca tctgggccgg
     1081 aaaatcggca gtcaccacga agtgtacggc gaggtcaaat ttatgccttc gcagcttgag
     1141 tggaatatca ttcccgggta cttctaccgt tttacggccg gtaccgagat gggctatcag
     1201 tttgaaagcc tcgacgacag ccatcacctg tggtttcggc agccccttgg cagccgttgg
     1261 gccctgcggt atgaccgcga tatgacgcat accgaaaatg aaataggtct tcagtatcgt
     1321 gtggacgatt acgtcggctt agagtatgtc gtatccgacc atgatcattg gcttcgaatc
     1381 ataggttatt tgtaagataa aggagatttt ctattatgat gtctgaaatg ggtaagaaac
     1441 gcaatattaa aattttctcg gcagtcattg ccgctatctt tgtattcagt atcgccggtc
     1501 ttgctatcat gcagacgggc aatccggtaa atgcagcgcc ttcgagcaat atcggcgtcg
     1561 tcgataccag caaaatcatt acgccggata accaggatgc cgttgaagta cagaaacagc
     1621 tccagcaggc cgaagcagat atgcagaaac agtttgaaca gcagtcggcc aacatggatg
     1681 atcagcagaa acaggaactc ttccagaaga tgcagggcga actgactacg aaacgtcagg
     1741 aactctttaa aggcctgaaa gataaggttg atacggctgt cggtaatgta gcccagacga
     1801 aaggcctcag cctggttgtt gaaaagagcg tcgttctcta tggcggcacg gatattaccg
     1861 atcaggttac gaaagaactg aaccagaatt cgagtgcttc ctccagcagc accagcaaca
     1921 atcagtaagg agttcttatg aaacggatat atgcggcagc cggtgccgtg gttctcttcg
     1981 tttgcctctt ggccggctgg tggtttttca accatcggga tcaatccgtg ccagcggcag
     2041 cagcgacgcc ggtctatgcc tatgcagact tggaacacgt cgtcatgagc catccgcgct
     2101 acagcgagta tcaccggtta gaactggaat acaatgccat ggttgcccaa tatcagtttg
     2161 aacaatggaa ttattcccgt aaggctcaag ccgaaggcgt gtcgatgcag cagtttgcgg
     2221 ctgtcgatgc agccggtacg gcggctctca atcaggaatt acaggctaaa gtcgccatca
     2281 agcaggacga gctcaacaac cgtttaaaac agcagtatga cgccctcgtc gaagaaaaga
     2341 aaaagacgac accggtcata tcagatgccg atgccttaaa aatcgtcaat ctccagctga
     2401 agctgaagac cttagacctt tcaaaagaag agcgggcagc gacggaagaa cagctgcaag
     2461 ccctcttgag aggcagtaat gcagaggtgt tggtgactgg cagtacggcc aatgaaatcg
     2521 ctgcggctat ggagccctac aaggctcagg ctaagaagga actcgaagac tatgcccagc
     2581 aggtcaagac cgaccttgaa gggcgcaaac ttcagagtca agcggttttc caacagcaaa
     2641 tgggggaact gaaaaatcgg cccgaaccgg ctgtgtggaa taaagagtgg aaagataagc
     2701 tcgatgcaaa agaaaaagaa atgaacgatg tgaaagaagc catcatggca gatatccgtg
     2761 ataaggcggc tgccgttgct caggaacagg gcatcgacat gatcttttcc gactatgaag
     2821 gtgtcggtac agccgccgat gtgactgatg atatcattgc caaattggca taacaggagg
     2881 cttttaggaa tgaaaaaagt ttggaagcgc tttggaatcg tcgccgtcat ggtagccctt
     2941 acaggggctc ttgccggctg cggcggtaaa gataatgtag gtgttgtcga tatgtcgcgc
     3001 atacagaaag aagcgcccct tgttcagcaa tataagcaga agacggaaga taagcagaag
     3061 gctatcgata aagaactgca ggatgcgaaa cagaccatgt ctgctgaaga ttttcagaag
     3121 aagcagcagc aggctcagca ggaactcaat attttcggcg ccagcatgca gcgtcaattc
     3181 atgtcggatc ttgaaagccg gttaggtgat atcgctaagg ataaaaacgt cggcatcatc
     3241 gtcatgaaag aagccgttcc ttccggcggc atcgatgtta ccgatgatct tattgccaaa
     3301 atgcagtaaa aaggggttgt atatagtgaa taaaacagta caggaattag cagtattgct
     3361 gggcgggacg gtcatcggca acggtgatgc cgtcattggt gacgtaaaaa gcctggaaga
     3421 agcaggtcaa aacgatatta cctttgccgt tgatccgtat acggaatatt tgccgcaggt
     3481 ccatgtcggc gccgtcattg tcgacaaaga agtaccggcc ggtgataata cactggtcat
     3541 cgtagaaaat ccccgtttgg ctttttcgca gctcctggtc ttgtttcacc cccgtcagtc
     3601 gattacgccc ggtattcatc cgacggctat cgtcgatgag actgctgtca tcggggaaga
     3661 tgtctccatc atgccttatg ccgtcatcgg caaaaacgtt gaaatcggta aggggtctgt
     3721 catttatccc tacgtctttt taggtgacca tgtcaaaatt ggcgaaggct cggtcctcta
     3781 tcccggcgcc gtcatccacg aacactgcgt cctcggcaaa aatgctgtta tccgggctca
     3841 tgccgtcatc ggtggcgaag gcttcggctt tgccacggaa aaagggaagc atacccgcat
     3901 tccgcagatc ggaaacgtca tcctcggtga cgacgtcgaa attggcgcct gcacctgctt
     3961 ggacaatgcg accctggggg cgacgaaggt tggtagaggt actaaaatcg ataacctggt
     4021 ccacctgggg cacaacgttg aaatcggtga agattgcttt atcattgccc agtccggcat
     4081 tgccggcagt accaaggccg gcaatcacgt tatctttgcc ggacagacgg gctgtacggg
     4141 ccacgttacg attggcgaca acgctgtttt tgccggtaag tcaggaatta tcggcaatat
     4201 caaaggcaat cagacctatg ccgggttccc ggcacggcct cacatggaat ggagccgcac
     4261 ccaggtattt ttgaagcatt taccggaaat ggccaagacc atcaaggacc tgcagaaaaa
     4321 gattgcccag ttagaacaga aattagaaca gcagtaagag gacaagctta tgtacacctt
     4381 catgaaatgt ctgagctgga ttatctgcca tcttcctgac ggctgctgcc gcagcctggg
     4441 cattttttta ggttcttttt tctggacttt tgtcccgaaa aaacgaaaag tattggctca
     4501 ggagcaaatt ttgaaatgcg gcattaccga cgatccgaac gaggccatgg ctattgccaa
     4561 agccagtaca cttcgctttg gtccgatgat catagaagtc ttacggtatc cggtctatac
     4621 gaaggaaacc ctcgatgaga aagtctcctt cgtcggccgc gagcatttag acgcgctcaa
     4681 ggaaagcggt gacggagcca tcatcatggc ttcccatgcc ggaaactggg agctcctcgg
     4741 agccgtcctt gccatgaacg gctatcccct catttccgtg gctcaggagc aagacagtca
     4801 tagtgccgat aagttcatca acgaatatcg ggctatgatg aagcagcacg ttacctataa
     4861 gacgggcatt cgcgatatgg tgaggctcct ccgggacggt cactatatcg gcctgctcat
     4921 ggatcaggac ccgggatata cgggtatcat ggttaaattc tttggcctcg atacgctgac
     4981 ggctgacggg ccggccaaga tgtccggtat cgccaactat cccatcgtac ctatctttat
     5041 ccgggaagac aggccgtacc atcacgtcgt tgaagtacag ccgccgctgc gcccttatac
     5101 ggcagatcac aagctctcaa aagaagaaaa agcgcgtatc atgtacgaaa cgacacagga
     5161 actcaatgac cgtttagaag cccatatccg tcaatatccg gaagactggt tctggttgca
     5221 caatcgctgg aaatggacga aacgttataa agaaaagcaa gctgaaaaag aagctgccca
     5281 atagtgaact gaccctctga cgtcggattt ttggtcccac gttagggggt cagttcccag
     5341 gcagcttctt tttttggtaa atattaacag aatgacgaca aatgtccgnn nnnnnnnnta
     5401 ttatgcgcgt tcttatgttt tttatagccc ttctttgttt cgtaacggca gggatcctgg
     5461 aaattaccaa tatcccgggc attcttgctt taatcgtcgg tcttgttgcc cttcggtttg
     5521 cctttcgggg gaagaagttc aagcctaaag tcgtcgtttc cagcggcaat gaagaactcg
     5581 ataaactcaa cacagacctc tttaagcggg ccgtcgaag
//
LOCUS       DAWDXZ010000051         5561 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4127_length_5561_cov_8.258082, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000051 DAWDXZ010000000
VERSION     DAWDXZ010000051.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4127_length_5561_cov_8.258082"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2575..2584
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3364..3373
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1491 a         1251 c         1426 g         1373 t
ORIGIN      
        1 acgttctgca agcggttact gacccatttg cgggtcaacg gcctgcggcc cttgggaatc
       61 tccctcgacg actacttcta taatcgggaa gacacgccga aaaatcctga cggcagttat
      121 gattttgaat cccttcgggc catcgacgtc cccttattca atcagcagat cgatgccctc
      181 aatcagggca aggccgtccg cttggctcgt tttgacttcg tcaccggtaa acggtacttt
      241 gatgatacgc cggttaccct ggaagcggga cagccggttg tcgtagaagg tctccacgcc
      301 ttaaatgatt ccctgaccta tatgctgccg cggtatgaaa aggtaaagat ttccttaggc
      361 gtcctgaccc agattcggat caacgaccac aaccggattt ctacatcgga tacgcgcatc
      421 atccggcgca tggtgcggga tcagcagttc cgcgaccgcg gtcccatggc aactatggat
      481 atttgggccg acgtccgccg cggagaagaa cagaacatct atccctacca ggaagatgcc
      541 gacaagattt tcaatacagc cctgccttac gaactgtccg tcctgaaacc ctacgccgaa
      601 cccttgttgg aatccatcga gaaggacagt gttcactatg ccgaggctca gcggctgttg
      661 aaattccttc gtccctttaa aggtgtcgac gcttccgtcg taccgcgaaa ctccctgttg
      721 cgggaattta tcgggcctga aaaaagaatt gacaaagcct aaccagatag atataatagt
      781 atagtcgagg tagaaatatg aaactacaag tcgaagaagc actaaaagaa gcggggaagt
      841 catttccctt ctcttttgaa tgtccggctg ccgaactggg tgatgtggac gaatttcctt
      901 ggaaagacgc caccgtagct atcgacggga cgtatggatt cgatggcaat catatcgttg
      961 tacatggaaa catacgggta tccggcggct acacctgcag ccgatgcttg tcaccggtat
     1021 tggtggatcg gaatgaaaca ctgtctgaag tctatggtac ggaagcaggg cttccagagg
     1081 acgtattgcc ctacaatggg gaatacatcg atttgacgga gacaattaga gaaacgttga
     1141 tcctcagcga gcctatgagg gtgttgtgcc ggcctgactg taagggactt tgtccgcagt
     1201 gcggtgcaaa cctgaatgac ggaccctgta gttgtcctac cgacaatatt gacccgagac
     1261 tcgacgtctt ggctcaatta ttacgatcca aagaagagcg ctagaaataa cgtaccatgt
     1321 gatctaagga ggtgcagacc atggcagtac caaagaacag attatcccag actcgtcaga
     1381 agatgcgtcg cgcaaactgg aaattgacag caccgggttt cgttgaatgc ccgcagtgcc
     1441 acgaagcaat gatgcctcat cacgtatgcc cgacttgcgg ctactataaa ggcaaacagg
     1501 ttgtcaacaa cacaacggcc gaataaggtt gtacataaaa aagccggttc atgaacatgg
     1561 atttcatgaa ccggcttttt tgtgtgcttt tttgtaggaa agaaagactg attccctgtg
     1621 aaatctgtac tccctttgag ataggtcagg gattggcctt tttgtattca tcgacttgct
     1681 ttttcaggca ttttaaaaag gcttcggacg ccctggacag gagctgatat tttttccagg
     1741 ctacgacaca ggataaatac cgggccggtt ctaaggggac ataggtgata tcgttatcct
     1801 gttgctgata aaggccggac atggccatgg cgttggtaag gcctcccttt atgagtaaag
     1861 aggcgtttcc tatgaggtca aaagagccga cgatattcag atcttcctgt ttgagggttc
     1921 ctccggacca ggattcaaag tcaaaggagt gtgtcgttcg gcgtgatgat acgagaaggg
     1981 gcatatcggc tatgatgtcg ggggtgatgg tcttgtaatg gctccacgga ctgtcacttt
     2041 tggtaatgat gccgacttgg tccctttcag gaagggcgag ggattcgaaa cctgacgcat
     2101 cgaaggggtc gatgaataat ccgaagtcga tcaagccgtg ggacagtttt tcttcgacga
     2161 tatcggcatt gcccgtaaag aggttgcacg taacctgcgg gtgctggagg tgaaggtccg
     2221 taaaggcatc ggttaagaag tgagcaatcc gcgtttcgcc ggcaccgagg cggatacagc
     2281 ccgttaagtc ggtctggtca tcgctgattt cagcttctgt ttgacggacg agagacagga
     2341 tttcctcggc tcgttggcgg aggtggatgc catcttcagt cagtttcgtg ttgcgattgg
     2401 tccggatgaa gagctgcttg cctaactcct gttctaaatc ggctatttga cgcgataagg
     2461 tcggttgggt aatgtgcagg gtgttggccg cttcggtcat gttttcttct ctggcgacgg
     2521 ccagaaaata ttctaaagtc cgtatttcca tagcttcctc cttgcatatg acatnnnnnn
     2581 nnnnttacat tcctgactac ttttgttaag ataagagtgc ctcaagggag aggtgttcga
     2641 cacgtcgagc gatgaattcg cttctgctgc agttcttaac ggaagggatg ggaagaaagg
     2701 aatccgtctg aaaatccttg gaatacattt tgaaaatgtc taggtattga atataagact
     2761 tttacattcc tgactacttt tgttaagata agagcgtccc aagggacagg cgttcgatac
     2821 gtcgaaatat aaatgcgtgc cttctgattg tttttgacgg acagcacgct ttgaaataag
     2881 aaacatgcct gaagcgtatg tctggaaata agatataagc tttttacatt tcaaggacca
     2941 tctgctaaga taagtccttg taagacgggc aagaagggag gccatgagga tgggacctga
     3001 atcatggaag agtttagtcg atgtcggctg ctcagcggaa tgcattgaac agtataaaag
     3061 attgactgac gatgaacagc gcttccttta cttgcgtcag taccgacggt gcctgttgaa
     3121 taagatccac gacaagcagc agcagctgga ccgcctcgat tacttactgc atcagttaaa
     3181 aaagggaggc tgattatgag tttattaatt gtctactatt cttggtcgaa cggcaatacg
     3241 caaaagatag ccgaacaact tcgactggcg acgggcgccg atatggcccg cattgaaacg
     3301 gtaaagcctt atgaaggcga ctatatggac gtcgtcaatc agggccagcg cgaagtgaat
     3361 cagnnnnnnn nnncggacat ccgcgagctg gcgcacgacc cggctgacta tgacgtcatc
     3421 gccgtcggaa cgccgacctg gtggtacacc atggctccgg ctgtaaaaac ctttttggaa
     3481 gacaatgatt ttgcgggtaa gacggtcatt ccttttatga ccaacggcgg atggccggga
     3541 catgtcatca aggatatgaa gcaggccgcc aagggggcct cgttcggacc ctctatggaa
     3601 gttcagttcg acagcagcgg cggcagtgac ctggtaactg atattggaga cgttgaccgc
     3661 tggataaaag atattaaaca gacatatagt atgtaggagg acatggatat gacattagaa
     3721 gaactcaaga aaaatagcat ctttgccgta ggcggagaaa acgtagatta tgcacagtac
     3781 tttgacggca tgagttactt gaatatgctg tccttgaaac aagtcattgt cggcaacgtt
     3841 acctttgaac cggcttgccg caatcattgg cacatccatc atgcctcaga agggggcgga
     3901 cagatgctgc tggtaacggc cggccgcggc tggtaccagg aatggggcca accggctcag
     3961 gaactccatc ccggcgacgt cgtcaacatt ccggccaacg tcaaacactg gcacggtgcc
     4021 gccaaagaca gtgctttcca gcacttggcc attgaagttc ccggtaagga tacgagtact
     4081 gaatggtgcg aagccgtttc tgaagccgat tacaatcagc ttccgtaagg cggtgcccta
     4141 tggtcattaa aggatatatc aaattggttt tggctttgag ctttatgctg ctggccggaa
     4201 cgctgtttat gggaacggaa ggagcctatg ctatgagcaa cgatacacaa tctaaaaaag
     4261 ccgtaaaaat cgtacagact gccggtcgcc agcggttagg tgattttgcc cccgattttg
     4321 cccgctataa tgatgacatc cttttcggag aagtctggag taagaacgat gtcctggcct
     4381 tgaaggaacg gagcatcgtc actgtttcgg ccttgatcgc tcagggcctt gtcgattcat
     4441 ctttgaagtt ccatatggaa acggccaaga aaaatggcgt gaccaaagat gaaatggttg
     4501 aaatcattac ccagctcgct ttttacagtg gatggccgaa agcatgggcc gcattctcct
     4561 atgcgaagga agtctacgga gatcagtaat gcaaagcgtt cttaattatt aatgacagcc
     4621 agtggttgcc attaataatt ttaagaaaaa atatcaaaaa tcaattcatt gtaatattga
     4681 tatatctcga aatgtggagt ataatacaac catgctaaag attgattttg aattagtaat
     4741 ggtttttaca tagaaggaga aataaattat gaaacaagga atgttcaaac aagtattatc
     4801 agccatggtt ttgaccatgt ccctggccgg aggcgcagcc tttgtttcgg ccgattccat
     4861 ccagtacagt gacaatggcg attataaagc cgctgttatc gaaacggccc cggcctcgac
     4921 tccggtagtt gccagccgag tctacagtga acataacgac caccaggccg tcatcaactt
     4981 ggatgcaccg gcagccaaca tcgtagccgt tcgcggttac agcgaaaacg gtgattccgt
     5041 cgcagcatcg gctttcaatg cctaacgaga ttacataaaa cgatacatta taaatttaga
     5101 aaattctgac catgtatatg acgatggttt ttatatagaa ggagagacac tataatgaaa
     5161 aagaaccttt ttaaacaagt attatcggca gtcgtattga ccatgtcctt ggcaggcgca
     5221 gcctttgttt cggccgattc catccagtac agtgacaatg gcgattataa agccgctgtt
     5281 atcgaaacgg ccccggcctc gactccggta gttgccagcc gagtctacag tgaacataac
     5341 gaccaccagg ccgtcatcaa cttggatgca ccggcagcca acatcgtagc cgttcgcggt
     5401 tacagcgaca acggtgattc cgttgcagca tcggctttta acgcctaatg agattacata
     5461 aaacgataca ttatagacaa agaaaattct gaccatgtgc atgacgatgg tttttatata
     5521 gaaggagaga ccccattatg aaaaagaacc tttttaaaca a
//
LOCUS       DAWDXZ010000052         5550 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4133_length_5550_cov_9.350318, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000052 DAWDXZ010000000
VERSION     DAWDXZ010000052.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5550)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4133_length_5550_cov_9.350318"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1041..1050
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3234..3243
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4050..4059
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1302 a         1558 c         1413 g         1247 t
ORIGIN      
        1 gccgcaaaat ctgccaggtc gacgtaccgc aggaacgggc agccatgacg tattcctgct
       61 gtttcaggct gaggatctgg ccacggacga cacgggccat attgagccaa taggcgatac
      121 ctaaggctaa ataaatgttg atcagccccg gtttgaaaac gaccatcagc aaaatgacgt
      181 agagcagcat gggaatggag taaaggatat cgacgacgtg catcatgatc cggtcgatcc
      241 gtcctccggc caaggccgca atcccgccgt agagaacgcc gatgaagacg ttaataaggc
      301 tggccaccag gccgatggac aaggaaatgc gggcaccgta caagacgcgg acaaagaggt
      361 cccgtccgag tgagtcggtt ccaaaccagt gtgctgcaga cggccaggca ttggcatcag
      421 aaaaactctg atcggcataa gtatagggtg ataaaaaagg tccgatgacc gcaaaaaggg
      481 tcatgacgat gacgatagcc atgccccaaa gggccaaggg attgctcttc atccgacgcc
      541 aaccgcggtc cctcagcgtt tgaggtgcca catagggttc ccttcggtcc gcctccgtaa
      601 tcggtgtgaa atccatggct attcctcctt tcggctgtcg acggtaatgc gcggatctaa
      661 gagcggatag atgaggtcga cgagtaaatt catcatcatg ataagaaaac tgtaaaaaat
      721 agtaatcccc aagatgaccg tgtaatcgcg gttataaata ctggtaacga agtgacgccc
      781 caatccggga atggcaaaaa tcgtctcgac gataaagctc ccggtcatca aggccgcagc
      841 caagggcccg atataactga cgacgggcag gagggcgttg cgcagggcat gacggtacaa
      901 taaggaccaa gaaccaatgc ctcgagagcg agccgtccgt atataatcct gtcccaaggc
      961 atcgacgagg ctagaccttg tcagacgcat aataaaagcc gtcggctgcg cagccagggc
     1021 caaggccggc aggatgacat nnnnnnnnnn ggacctttcc acaaagccgc cggcaggaca
     1081 ggccaagtaa aagcgaagag ctgtatgaga acggcagcaa aaataaaact ggggaccgaa
     1141 acgccgagag tagcgccgat catcatcccg taatcaaccc aggtattctt ataccaggca
     1201 gccgccatgc cggcaagaat accggccccg atggcaagga gaaggctgat aagcccgagc
     1261 tcagccgata caggcagggt ttcggcgatg atatcgttga ccgttttacc gggatatttg
     1321 taagacggac ccagatcgag aacagcggca tggcggacgt aatcgacgta ctggttccac
     1381 aagggttcat cgaggtgata ccgggcttcc accgtcgcca tgacagccgg cggcagtttc
     1441 ttttcctgcg taaaaggacc gccgggaatg gcgtgcatca gaccaaaagt aatggtaatg
     1501 accgcccata ggacgataca ggcgccaaag aggcgctttg cgagataaat ggccaaggag
     1561 aatcattcct ttccaacata gatactatta attataaggc cctatcttta tcatgacaag
     1621 gcaatggccg ttgttattat cctggcggcg gcgtataata gtaaattgac tacgttaaca
     1681 aatcgaaaga aaggtgatcg ctgtgaactg gaaactttgt ttagcaattc taacgtgtaa
     1741 cgtcgtcttc atgtcctcaa gctacaccat gctcatcccc ttcctgccta tgtatcttac
     1801 ccgagacctc ggcgtcgatc cggcgtcggt caacatttgg tccggtatcg tcttttcctc
     1861 gacctttctc atcagtgccg tcatggcccc catatggggc cggatggccg acaaaagagg
     1921 caaacgactc atggcgattc gcgccagttt tcttttatcg atcagttatt ttctcggcgg
     1981 tatcgtaacg acaccgctgg aactgacttt catgcggatg ttccaaggct ttgcgtcggg
     2041 tctgtggccc atggaactgg ccatcatgac cacctatgct ccacccaaaa aactgggcat
     2101 ctgcctgggc gtcatgcaag gggccctcac agccggcggc atcatcggcc ccctctttgg
     2161 cggcatctta gccgaaacct tcggcatgcg ccaatccttc tttttagcag ccgccgcttt
     2221 gttccttaac tttttagtct tggtcttctt tattaaagaa ccgccgacgg acgcggcacc
     2281 gtctgccgat accatcagtg ccgacagcaa aagcggccaa gtcagcctgt ggaagacgcc
     2341 tgtcatccgc ctgatgctca tcagcgccgc cttagtgcag gctgtcatcc tcatcgtcca
     2401 gccgatttta acgacctata tttcttacct tgccggagac atggacaacc tggtcttcgt
     2461 agccggcctt atcttctccc tgggcggttt tgccagcgcc attacggctc ccctctgggg
     2521 ccgtttcggt cagcgccacg gtttcgtaaa gaccctgcgc atcgtactgg ttctggcagg
     2581 tattttcttc ctcatccagt cccttcccga tacgctttac accttcgcgg ccagccaatt
     2641 cgccgtcggc ctcttcttct ccggcatcta tccgtcgatc aatgccatct tagcggaaaa
     2701 gacgagtgcc aatatcaaag gccgggtctt cgggctcatg ttctcggccc agcaggtcgg
     2761 cgccatgggc ggcccgattt taggcggcat cattgccacc tatatcggaa tgaagtacgt
     2821 cttttgggga gccggcacga ttctcttgtg cctcagtctg gccatcacct ggaaggtaca
     2881 ccatctgcac ggtgaaaaat tggcggatta acacttaccc ccgggtaccc cgattatggg
     2941 tgtagtccgg aaggagtttc ggcgataata ccttttcggt aatgccggca gcggcacgtt
     3001 cttcatcaaa gcgacgaacg tggtcgagat tcccctttcc gggatgggcc tgctcactgt
     3061 agaccgccgc ttcggtcccg gcaatccggc caaagacgat gatatccaag agactgttgc
     3121 ccatgagtcg gttcttcccg tgaataccgc cgacgtcttc cccggccgca tagaggccgg
     3181 ggatttttgt ttcactctta gctccgatac agataccgcc attttgataa tggnnnnnnn
     3241 nnnaaaatcg gctgttttcg gatatcgatg ccgaattttt catacatatg aatcatagcc
     3301 ggcagtcttt tttccatcgt cccctcccca tgaagaatat cgaccatagg cgtatcgagc
     3361 cagacggctt ggccgtgcag ggcatagacg cccttgttcc gttcttggca ctcgcgaata
     3421 atggccgcgg ccacgacatc tctcgtttct aaaggatgga ggaaggcatc gccgtcctta
     3481 ttgaccagca tggcatcgag agaacggacc ttttccgtga ccaaggctcc ataaatctga
     3541 ggcgggtaag cggcccctgt cgggtgatat tgaatagcgc cggcataaag gagagggacg
     3601 ccggcccgat aggccatgac cagcccatcc gccgtcgatc cataatgatt cgacgtcgga
     3661 aagccttgat agtggagacg cccggcacca ccggcagcaa ggatgacgct ttttgctcga
     3721 acgacgtggt attcccgggt atcgaaatca tacaagacgg caccggcagc ccggccttcc
     3781 ccgtcaagca gcaattcgac agccggcgaa tattcgatga catcgacgcc ggcgttacgg
     3841 acttcatcgc gaagaacacg catgatttcc gctcccgtat aatccccgca ggcatgcatc
     3901 cgtttatagg acgtcccgcc gccgtgggtc gttaccatgg ctccgtcacg gtctttgtca
     3961 aataggacgc ctaaggagct gagccactga atggcttccg gcccatgaag aaccagttca
     4021 gagagcagtt cgggaatatt cttataatgn nnnnnnnnng accgccgccc atggcgtcca
     4081 aataatgagc cgccggcgaa tcattttcct tatcggcagc ctgtatgcct ccttcggcca
     4141 tgcccgtatt ggcatctccc aatcgtagtt tcgtagccat gaggaccttc gctccatggc
     4201 gggccgcttc aatagctgcg gcagcaccgg acccgccgcc gccgatgacc aggacgtcgg
     4261 tctcaaactg gatatggtcg acatcgacgt cggcttcatc catgcggctc tggccttgta
     4321 agaggacggc cagttcagac ggtacttgat cgcccttatt gggtcctacg gacaggcgtg
     4381 taaaaccgtc ttcgtgataa tcaggatgaa attgttttaa aagggcatcc ttttcttcag
     4441 ccgtaaaccg cggcaatgtt tccgacaagc ggatactgcg cgtcgcctcg acccgcgcct
     4501 gagacgcccg catttcttcg gtatatatca tgaggtcctc ctatttttca atcgtccgcc
     4561 cttcgtataa gcggcgcaat tcatcgtcat cggcttccat caaccgctgt acatcggcaa
     4621 tatactgccc ttcttgaatc gcttttacgc ggtcttccaa atgacggctc tttttggcaa
     4681 tatacttacc catgaggcgc cgtgccaaga ggccgaccgg tccgtggctg attttcgccg
     4741 gacagctggc agcacagata ttgcagcaaa cacagcggaa ggactctttc gcacagccgg
     4801 cataatcacc tttctgggcc agggcgatgt atttcatgac ttgaatccct tgaggacagt
     4861 tccgcgtaca ggcattgcag tgaatgcagt tataaatctc aggataaagc tgaccgacga
     4921 catttccggc aacaggcaaa tcatccatgt catattccat ctttttgatg ggaaacgatt
     4981 ccaaccggcc gacacacatg ccgtcttcaa ccttcgtttg acaggccagg gccgaatgcg
     5041 tttcagtctg ccctttcaga cggtagacga cggcgcaggc cccgcaaaac ccgcttcggc
     5101 atccgcagcc gcgaaccagg cggaagccgg agtattccat agcccgcata atcgtcaaat
     5161 cttcaggaac cgaaaacatc ttccccagta aatagacggt taccattttc atagctattt
     5221 ctcctttaat gaaaaaggtg ctgccgcaca tgcggcagca ccttttattg ctttataaac
     5281 cgtaatctta ttccaatact tccagcaagc agtcgccgct gatgatagcc tggcctttga
     5341 cggcatagat ttcaccgaca gttccgtcga taggcgacgt aatcgtcgtt tccatcttca
     5401 tggcttcggt aacgactaag ggttcgccct tcttaacttt ctggcccttg gaaacgagga
     5461 cattgacgac cgaaccggac aaggtggcac cgatgtcacc ggccttatca ggatctgcct
     5521 tacggcgaga aacggtcttc atgtcgacgc
//
LOCUS       DAWDXZ010000053         5514 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4157_length_5514_cov_8.904012, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000053 DAWDXZ010000000
VERSION     DAWDXZ010000053.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4157_length_5514_cov_8.904012"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    416..425
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1383..1392
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5001..5010
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1368 a         1413 c         1301 g         1402 t
ORIGIN      
        1 gtcgccgctg actatccgca gattgaaagc gatgataaaa tcgtcgacaa cacctgcatg
       61 cagctggtca tgcgtccgga acagttcgat gtcatcgtca tgccaaacct ctacggcgat
      121 attatttccg accttacgag cggcctcatc ggcggcctgg gtctcctgcc ttccatgaat
      181 atcggcgacg attacgccat gttcgaagcc gtccacggca gcgctcccga tattgccggc
      241 aagcacattg ccaatccgac ggctcttctt tggtcagcct gcatgctcct tgaacatatc
      301 ggcgaaacgg ccattgccga aaagattcgc aaagccgtcg atgccgtcct ggcagaaaag
      361 aacgccctca cgccggacct tggcggaaag gcgacgaccg aagcctatcg cgacgnnnnn
      421 nnnnngccgt agctgccaaa ggctgcggcc ttcttgtttt taaacttcag accgcgaatc
      481 atttccatga taccgccgat agagtagaga aatccgtagt tgattgtcgg tgagccgact
      541 aaaatagcct tcgatttgaa gacttccgtg acgatatcgt tcttatcgtc gacggctgaa
      601 ttatacaact tgaccgtcag cgtcggatcg gcctcacgaa ggccttgggc aatcgattca
      661 gccatgcgcc gcgtggcgtt ccacatcgta tcataaatga tggtaatctg attttcctga
      721 tattctttag cccacttctg atacgtttcc acgatttgca ggggattatc gcgccagatg
      781 acgccgtggc tggggcagat catgttgacg ggaacgttca tctttaagat ctcatcaatc
      841 ttacggatga ccatgggact gtagagattc aagatattgg cataatactt catggcttca
      901 tgatagagtt ctgcctggtc gacgcagtcg ttatacatcc attcgctggc aaaatgctga
      961 ccgaagccgt cattactgaa gaggatattg tcaccggtca tataggtaaa catcgtatcc
     1021 ggccagtgga gcatgggcgc ttcaatgaat accagcttac tgtcgcccag atccagggta
     1081 tcgcccgttt tgacgttgac gtaattccaa tcttggtgat acagcccgcg aagaatggct
     1141 tcgccttttt gggtacagta aatgggaacg tcggggattt cccgcatcaa agccggcagt
     1201 gccccactgt ggtcattttc attatgattc atgacgatgt aatcgatttt attcaagtcg
     1261 atttcctgtt ttaagtaatc gacgaattct tcatcgaagg gtttccatac cgtatcgatc
     1321 aaggcgactt tctggtcgcg aactagatag gaattataag acgaaccctt ttccgtcgaa
     1381 tannnnnnnn nnattcatgg ccgtgaaact tattcagttc ccaatcgatt tttccgaccc
     1441 aagtaacttt ttctgtaatt tttcttccca taaggcatac tcctttcgcg gcagactagc
     1501 tgctgactca cgacataccg aaacaagagg gcttatcact gccctttctg agttcagtat
     1561 atccaatgag gggacaaata tccattgcaa ttccaacaaa attcaacatc cttgccaaaa
     1621 aagtatactt tggttaaagc ccttatccat cgtcacaaac tgtctgaatc gatgaatcca
     1681 tgttataatt taattacaaa aacttattct gaggggagaa accactatga atatcaccgt
     1741 cttaggctgt ggccgctggg gcagtttcca tgcctggtat gccgaccata tcggtcatga
     1801 cgtgacctta tggggacgac cgggttctaa aaacctgagc cgcctgaaag aaacaaggaa
     1861 aaatgattat cttaccttac cgaacgcaat caccctgacc gacgacctga aagaggccat
     1921 gtccggtgcc gatatctgca ttatttctat cagtgcccag cagcttcgtt cctttgccaa
     1981 ggatattcgg agcgccggat acgacaagca cttgcccttc gtattggcca tgaagggcct
     2041 cgaagccggc agcggcaagc ggctgacaac ggtaatggca gaagaacttg gggaggccgt
     2101 acggactgcg gtctgggtcg gccccggcca tgtccaggac ttcattagcg gcatgcccaa
     2161 ttgtatggtc atcgcctcgg cagacgatga gttgacccat ttcttagtcg atacttttac
     2221 cagcccgctg atccgcttct attacggcca agatcttctc ggcattgaaa tcggagctgc
     2281 tgccaaaaac gtcgtcggta ttgcagccgg catgctcgac ggggcccata tgacggcctt
     2341 aaaaggtgcc ctcatggccc gcggtgccca tgagttatcg gttttagtct ctgccatggg
     2401 cggccaggcc atgaccgtgt acggcctcag tcacttaggc gactatgaag cgaccctctt
     2461 ttcgccgcac agcaacaacc gccgcttcgg ccaagatttt atcgaaggaa acactttttc
     2521 aaaacttgct gaaggcgtca gcacgaccga agccctgtta gatctatcgg cccactacgg
     2581 cgtagaactg cccattacgt cggccgttca ttccatcata caggaacatc aggaccctca
     2641 tgaagtctta aaaaatctct ttctccgctc gaccaaaagt gaataagtac gacaaaagga
     2701 gctgtaccca ttgattgagg tacagctccc ttttttcatg cctttatttt tcttcttcat
     2761 catcttcaga ataatctgga ttgcgagtga cgaggatttt atcgacgcgg aggttatcca
     2821 tatcgacgac ttcaaaggta aagtccttcc atacgtaggt atccgtttcc cgtgggatcc
     2881 ggccgacacc ataggtaatc aggccggcca gggttttata caagtcttct tcttcgtcag
     2941 gcagttcatc ttcgatgccg aagaaatctt taaattcttc aatcgtaagc agtccttcga
     3001 taagccagtc gttcggtccc cgttggatga tgcgattggc ttcttccttc ttttcttctt
     3061 ccccggccgg catgatgccg actaattctt cgaggatatc gtgaagggtc aggatgccgc
     3121 taaagtttcc gtattcatca aggacggcga cttcatgaac gctgtgttcg cgaaagacgt
     3181 ggacgacttt catgatgtcc atcgattccg gaatataaat cggtttgtgg acgcattctt
     3241 ctacaataac aggcagagac gccggttttt gatggcgaat attttcataa tacttctgaa
     3301 agatttcact gaccgatacc atgccgatga agtcatccaa agacccccgc ccgacaggaa
     3361 gattattgtg attgaaagcc accaattcat tcatcatcgt cgcttccgga tcttccacgt
     3421 cgagccaatc gacttgggtc cggttagtca tgatgtcgcc gacgtccata tcggccaggc
     3481 gaaagacgcg ggtgaccagc tccggttctt ctttttcaaa agccccgagt tcgacgccct
     3541 gctctaagag caattttatt tcgtcttccg taaccggcag cacttccggc ttcttgacac
     3601 ccatcatttt ggtgacagcc gtcgtcgacc acgacaggat ccagacgaca ggcgtacaaa
     3661 tggccgaaaa gtacagcatg ggacgggcca gcaagcaggc tactttttca ggattgtcaa
     3721 tggcaatccg tttaggaaca atttcaccga ggataatcgt caaataggta atcaatacga
     3781 cgacactggt cagactgatc gtataggcat acggttcaag gcctggaatc ttggctacat
     3841 aaacggataa agggccggcc atttccgtac cgccgtagac accggtcagg aggctgatca
     3901 aggtgatgcc gaactggacc gtagaaaaca tcttattcgg attttcacgc agtttcagga
     3961 cgatagccgc cgacttgcta ccttcatcgg caatcgcttc caatcgagat tgatgactgc
     4021 tgacgatagc catttccaat aatgaaaaga cgccgttgcc aaagataagt aagatgatga
     4081 ccagtaactg ttttcctagg gaaaactcca cagtagatac actctccttt tagattacca
     4141 ccaaggtcct acaccgatgc cgattccacc gccatgatgc catccgctgc cccagccaat
     4201 accgataccg acaccgacgc tgggattatt gcgggacttt gattctctgc ggtccgcttc
     4261 atcgacgacg gtaatttctt tagtcaccgt gcgaagggaa tcgccgattt tcacatcgac
     4321 gacgagatcg actttccccg cttccagcgg gcggatattc atatgctccg taatatcggc
     4381 aattttcgta ttgaggctgg tcgccttgat atcataggga atcattcccg gtacatgaac
     4441 gcggatgata ccgtaactgc caagagggat tttttgcgga tacacgacga cttcaacctt
     4501 atccatcagc ttttcggcac tgccgtagat aagggatgcc tgctggtcgt tctgaagctt
     4561 ccaggcactg ctctcatctt tagccacaaa atagtttccc tttaagacgt gggagtccgt
     4621 atcgtaagct ttgacgtgat agaagtagtc gccgacgacc tgcttgtcaa cgcgaaaata
     4681 ataatcgcct gtaaggcctg tcgtctgcgg cggcaaggcc ataagcaatc cggttacctt
     4741 gtcataggta cggccttcaa gaatataaaa atcactcaaa ttcttcatat ttaccgtcgg
     4801 atcagtgagg atgacggctg acgactgtcc cgtctgcgta ccttccggaa cgccgtcggc
     4861 caggactgtc atcgtactga ctgtcagaca aagggcagcg agaagcaggt gccatttttt
     4921 catcggtaat ccctccttat gataaggtaa cgagataaat tattttttta gtttatgaat
     4981 gccttccaga cgatgcatgg nnnnnnnnnn tcggctaagg tttcatcgcg gacggcaaac
     5041 tgcaatcttg ccaagtgatg gacaggttct ttaaaaaagg tcgatgccgg aacgagggcg
     5101 acaccgattt tttccgctaa atcccggcaa aaggcttcat cgtcatcata cccgtaggta
     5161 ccgatgtcga ggagcatata aaaagctcct tgagggacgt tatgagctat gccgatggca
     5221 tcgaggccct tcatgacgaa atctcgcttt ttccgataga gctctcgaag gcactgatag
     5281 tagtcaggac cgaactccag accggttaca gccgcttctt gaaggggact gggagcactg
     5341 atcgtcagat agacgtgcag ctttttaata gcatcggtta ctgatggaga ggcaaagata
     5401 tagcctaacc gccagcccgt aatcgaatac gtcttcgata gggagccgca ggaaatggtc
     5461 cgttcataca tcccgggaag ggtcgaaaaa taggtatact ccgtcggttc gtag
//
LOCUS       DAWDXZ010000054         5512 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4159_length_5512_cov_9.840205, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000054 DAWDXZ010000000
VERSION     DAWDXZ010000054.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4159_length_5512_cov_9.840205"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2007..2016
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3681..3690
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1337 a         1575 c         1616 g          964 t
ORIGIN      
        1 ctgcttgttt tcgatgatga cgtcttccag gaggtcttcg tcttcttcat acattttaat
       61 gagatggtga agggccgtgt tgcggcgcag gcgcatgagg cgctccatga cgatgccatt
      121 gcccctgagg gccgatgtga agtaggtcaa ggatttctgt aattccaaga gcatgaaaaa
      181 ttccttgttg cgcatggaat ggcgcagtgt ttcttcgaga tcatccgttc ggtgattgat
      241 ctgcattaag taacgaagga ataaggttgc cgaacgatag agtatttgaa ataagaagcg
      301 cgtctgttta atggtgtgga acaggggata ggtgtggagc tgagaggctt caaatccttt
      361 gccgataaga caattttgaa ctttgataag ggtattacgg ccatcgtcgg gcccaacggc
      421 agcggtaaga gcaacatttc cgatgccgtc cgctgggtca tgggggaaca gaacatccgc
      481 caactgcggg gccagcgtgc cgaagacatc atctttgccg gtacacaaac ccgccggcct
      541 cagggtgcgg ccgaagtatc gctctatttt gataacagcg accatgccct ggatacggaa
      601 tttacggaag tcgtcgtcac ccggcgcctc ttccggtccg gtgacagtga atactatatc
      661 aaccgtcggc cgtgccgctt aaaggatatc catatcctct ttgccgacac cggtatcggt
      721 caggattcga tggccgtcat cggccagaac cgcgtcgacc gcatcttgaa cagcaagcct
      781 gaagaacggc gggtcatctt tgaagaagtg gccggcatca gccgttttaa ggggcgaaaa
      841 gccgaaggac tgcgaaaaat tgcggaaacg gaacgcaatt tggaacgcat cggcgacctt
      901 accagtgtcc tcgaagagca gctggaaccc ttgcagaaac aggctgaaac actgaaggag
      961 ttccgaaccc tcgacagcga acgcctggct tatgaaggga ccctgaccct ccaagaactg
     1021 cgcaacagcg aacgcctctt ggccaaagcc gaaaacagcc gccagtcggc atcggcggaa
     1081 gaacaggaag cggccaaagc attgaccgat gccgaacagc ggcgcaaggc cctcatggcc
     1141 tccatggcgg cggaagaaga aaaactgcgc cgcctcgatg aggaagccat ggccgtgcac
     1201 aacgaattgg acagcctgac ccatcgccag gaagcctgca ttcagcgaaa agccgaactg
     1261 gctgagcagc aggctgccga tgaaaaggat gaaggccagc tcaaagagcg gcagacgtct
     1321 tgggaggcca aggagaaaga actgcggcag cagaaggaac aaaagaaaac cgagctggca
     1381 gcggctcaaa aggccctggc cctgaccgaa accttagtca cccaggcgga cgacaaggct
     1441 aaacaagcag ccgacgccct aaagaaacgc ctcgatgcca attccagccg cagccgcgac
     1501 gccttcatgc tgcgctgcga ccgcgatgac ctgcgccgcc gcttagaaga aaatgccgac
     1561 gcctgtgaag aagcccttcg cgctttcgaa gaaaaaaagc aggccttgga agacttgaag
     1621 ggccgcagtc aggacgtcga agaccgaaga gaccagtggc agcaacgcct tcgagaccgg
     1681 gaaaaggatg ttgccgctca gaaggaggcc gctgccaagg cgcaggtcct cctgggggac
     1741 gtcgaacggg actatcgccg tcttcgcagt gacgtcgacg gggctcaaca gcgcatcggc
     1801 gtcttacgga gcatggaaca agaacacgaa ggcttgggcc gggccgtcaa ggtcgtcctt
     1861 acggcatcgc agccctggcg cagagagctt tgcggcgccg tcggcgaatt atgccgcatt
     1921 cccggtaagt ttgccgtcgc cgtcgacatt gccctgggcg ccgcggcccg gtacgtcgtg
     1981 gccgaaaacg aaggggcggc caaacannnn nnnnnntatg aagaaaaata taagacggtc
     2041 tttacgtctt tattggggaa gaccctcttg gccgatacga tggacacggg gtcggccgtc
     2101 gccaagaagt acggccaccg tctgcgcatc gtctgcctcg acggcacgca gttcaatgcc
     2161 ggcggctccc tgaccggcgg cagtacgcga aatcaggaag gctccctcat cagccgcaga
     2221 gccttgatgg aagacttgca gcagcacctt gacgacggtc aaaaaaagct ccaatggttg
     2281 acggaaaagg gcaaggcttt gcgccgaacg gctgaagacg ggaaacaggt cttggaggcg
     2341 gccgaagaag ccctgcaaaa ggccaagcag gaatcgggcc aggccgcatg gcaggctgca
     2401 cagctcgctt cggaattgcg ggaccttgaa aagaccgtga ccgatatgga taagcgcgtc
     2461 gaagcccttg acacggcccg ctcggatatc caagccgacc tcgtttccaa agagaaggcc
     2521 ctctcggccc tggaagaaga accggaaacg gacattcacc agtggcaggc cgctgccgat
     2581 gcagccaagg ccgaagcgga gcggtgccgg aagaacctga cggaatgtca gatcacggcg
     2641 gcgaccttaa aagaacaggt ccgccatggg gaagaacagc tgactcagca cgcttcctgg
     2701 caggacgaat tgacgagtca gcaggacatc cttgcgcaac ggcgtaagga ccttcgcgag
     2761 cgccgccaaa cgacggaaag gctcttacag gagctggctc agaatatcga agtgaagaag
     2821 gccgaaacgg ctcgttccga tgaggccaaa gaagctttct ataagagccg cagcgaacgg
     2881 ctgcagcaga gccatgacct ggacgaactc gtcgacgacc tgcgtaagcg cagtcagacc
     2941 tggcagcagc gcctcaatgc tgccgatatc cagatggaaa aatataagag cgatatcagt
     3001 catcatgaag aacggctggc catgcagggc ctgagtcggc aggaagccat ggatcggcgc
     3061 cgcgaaggct cgctgaaaga gcttcacgac aaggtcagtc agttaaaatc acaaataacg
     3121 gccttaggta ccatcaaccc ggctgccgaa gacgactatc aggcagccct ggataaacaa
     3181 gaattttaca agaagcagtg cctcgattta gaagaatcga gggaaaagct gcgggccgtc
     3241 gtcgccgaca tcgacaaggc catggccgac caattcggtc aggcctttca agatatcggc
     3301 gtccatttcc agcagatttt cagctgcctc tttgaaggcg gaacggccca tttggccctg
     3361 accgataaag aacacatctt agagtccggc gtcgaaatct acatccgtcc gccgggaaag
     3421 aaacagcagt ctctgaccct cctctcgggg ggcgaacgag cccttacggt catcgccctg
     3481 ctcctggcct ttttggcctt tcatccggcg ccgttctgtc tggtcgacga agtcgacgcg
     3541 gccctcgacg aagccaatgt agagcgcatg gccaagtatc tccagaatta cagcggctcg
     3601 acgcagttca tcgtcatcac tcaccgccgc aagacgatgg aagcggccaa tacgctcctc
     3661 ggcgtcacca tggaagaaaa nnnnnnnnnn tatcgcggct gctgtcggtc aaagtagatg
     3721 aattattaaa ggaaggaaca tgatcatggg atttttttca aaacttcgaa aaggcttaga
     3781 aaagacgaaa cactcgctca ttcaaaatat agagaccgtc gtccgcggct acgcgaaaat
     3841 cgacgacgac atgtatgacg acttggaagc ggtcatgctg accggcgaca tgggcgtcga
     3901 aacgacggaa tacctcctca atcagatccg gaccggcgtc aagaaccggg atatcaaaga
     3961 cgggaacgac gtcgtacctt accttgaagg gtgcatcgtc aagcttttga atgaaaacga
     4021 tgaacccctg ccggaaacgg aggggacacg ggtcatcttc gtcgtcggcg tcaacggcgt
     4081 cggcaagacg acgaccatcg gcaagttagc gaaatactat gaagaacaag ggaaatcggt
     4141 catgctggct gccggcgata ccttccgggc cgccgcgtcg gaacagctga ccatctgggc
     4201 cgaacgggtc ggcgctccca tcgtcaagca tcaggaagga gccgatgcgg ctgccgtcgt
     4261 cttcgacgcc acggcttcgg cgaaagcccg taacatcgat atcctcctgg tcgatacggc
     4321 aggccgattg cagactaagt ccaacctcat ggaagaactg cggaaaatgg cccgcatcgc
     4381 cggccgcaac atcgatgggg ctccccagga aacgctgctc gtcctcgatg ccacgacggg
     4441 acagaatgcc gtcagccagg ccaagctctt cggcgacgtc gtgcccttga ccggcgtcgt
     4501 cctgaccaag ctcgacggca cggccaaagg cggcgtcatc ctgtcgatta aacgggaact
     4561 gggcgtcccc gtccgctggg tcggcgtcgg cgaaggcgtc gacgatcttc gtcccttcga
     4621 tgccgcccag tttgccgatg ccctcttcga caagaacgtc gccgcccgtg aagagggtgg
     4681 cgaataagga gggaggaggt catgaccatc gtcgaagaca tgggctcgaa acttgcggcc
     4741 ctgcgccggg aacggcccct cattcatcac attacgaact ttgtcaccat ggacgactgc
     4801 gccaatgcga ccctggccat cggcgcctcg ccgacgatga ccaattccgt cgaagaagtc
     4861 gccgaaatgg cggcggccgc gaaagcactg gtcctcaatc tgggcaccct ccagcagtgg
     4921 accctggagg ccatgatcct ggccggaaag gcggcagccc gtaagggcgt tcccatcatc
     4981 ttcgacccgg tcggggccgg cgggacgacc tttcgcacgg aagcggccct gcgcctggtc
     5041 cgggaactac ccctttcggt catccgcggc aatgcggcgg aaatcacctg cctggccgat
     5101 cataagagcg gccgcaacct cggcgtcgac agcctggtcc gtcccgtcga cggtacgttg
     5161 gcggccgaac ttgccgataa attggcggcg accattgcca ttaccggcgc cgtcgacatc
     5221 atttccgacg gcacgcagac ggctgccgtc cacaacggct gccccatgct gacctatgtg
     5281 tccggcacgg gctgcatgac gtcatccctc atcggctcct ttgccgccgt cagcgacgcc
     5341 tttacggcag ctgtcgccgg cgtcacggcc atgggcatcg gcggcgaact ggcccttgaa
     5401 cgaggtggca gtgccggtcc cggttctttt cacgcccgcc tcttcgatgc tttttacagc
     5461 ctgaatggcg atctgttgca acagtatgga aaggtcttga ttacccatga at
//
LOCUS       DAWDXZ010000055         5509 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4163_length_5509_cov_8.471764, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000055 DAWDXZ010000000
VERSION     DAWDXZ010000055.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4163_length_5509_cov_8.471764"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2106..2168
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1226 a         1463 c         1416 g         1341 t
ORIGIN      
        1 tctccttctt taacagcggc accgacaggg gctacactga cgacgctgcc gctcaatacg
       61 gttatatagg atacatcagc cgcagctgcc ggcatcgcga gaagaccggc gagtccggct
      121 aatacggcga gtctgtaaag ttttttcatg acggctccct ccattcctat ttcactgaaa
      181 gagcgaaaca gttataaatc atcaaaacta tcttttaagc ggtccatttc tcggacggcc
      241 ttgccggcac catgggccac gcagagcagc ggatcttcgc aaagataggc tgccatgccg
      301 gtcgtacggg aaataagacg gtcaaaacta tcgagaagta atccgccgcc ggtgaggacg
      361 atgccgtggt cggcaatgtc cgccgccagt tcgggcggtg tcttttctaa gatggaaacg
      421 atcgtcttga ggacggtctg aatcggttcg tcgatggcca ggcggatttc ctgtgaatcg
      481 atgtcaatcg atacgggaag tcccgtttcc gtgctgcgcc cgcggacgtc ggccgtcatg
      541 cggcggccgt cactcatagc cgtgccgatt aagattttca gctcttctgc cgtgtgctgg
      601 ccaatggtga cgtgctttat cttttccaaa taacggatga tggcttcatc aaaggtattg
      661 ctgccgatgc gcagggattc actgatgacg atgccgctca ggctgagaac ggcgacatcc
      721 gtcgtgcctc cgcccatatc gacgaccata gacccgttcg acgtagcgat atcgaggccg
      781 gccccaatgg ctgctgccag cggttcttcc atgaccgtcg tctttctggc tccggcctgc
      841 atggccgctt cgatgacagc ccgccgttcg acgttggtaa cgctcgacgg gacacagatg
      901 acgacgcggg gtttaaaaaa caaggacttc ccaatggccc ggcggatgaa ataccgcagc
      961 atgtactccg tcgcatcgta gtcggcaatg acgccgttgc gaagggggtg gtaggcatgg
     1021 atgttgcgcg gcgtccgccc gatcatagcc tgggcttcgc ggccgatggc aacgacctta
     1081 ttttccatcg tatcgacggc gatgacggcc ggctcattga cgacgatgcc cttattttta
     1141 acgaatacaa taatgtttgc cgtccccaaa tcaatggcaa tatcactggc aaaccagttc
     1201 gataaaaaga acgagcggga atgccgtgcc ggctgtacct tttttttagt tttcttcttc
     1261 ctcaatattg acgcgaacaa tatctgcacc tagcctttgt aattttaata caaaattatc
     1321 gtaaccacgg tcgatgtggt atagataacc gacttccgtt tcgccttcag cgaccaagcc
     1381 cgccaggacc agggcggctc cggcacgcag gtcggtggca ttgaccgagg cgcctttaag
     1441 ctgttctatg ccttctacga tggcgctgcg tccttcgatt cggattttag cacccatccg
     1501 tttaaattca tcgacgtgca tgaatcggtt ttcaaagacc gtttccgtaa cgacgctcgt
     1561 tccttggcag agcgtcgtca acgccatgaa ctgggcctgc atatccgtcg gaaagccggg
     1621 ataaggcaag gtctttatat cggccggccg caagggctca tggccgatga cgcggatacc
     1681 gtcgatttct tcctccactt cggcaccgac ttcttttaac ttagaaataa ggggcttcaa
     1741 atgctctgaa atggcatttt caacgaagat gttgcccttg gtcatagcgg cgccgatcat
     1801 aaaggtaccg gcctcgatgc ggtcgggaat aacggcgtgg ctggcaccgt ggagttcctt
     1861 gacaccttcg atgcggatga cgttggtacc ggcaccgcga atattggcac ccatgctgtt
     1921 cagataggtt gccaaatcga cgatttccgg ttcttcagca gcattttcca agaccgtctt
     1981 gccattagcc atggaagcag ccatgatgat gttttccgtc gcaccgacac tggggaaatc
     2041 aaggtaaatc tgagcgccct gcagcccatt tggggctacg gcttcgatat cgccgttttc
     2101 aaggtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnga tgacggcacc catggcttca aaggccttga ggtgcaagtc aatcggacgc
     2221 gcaccgatgg agcagccgcc gggcagggaa atctgtgctc tccccttgcg ggccaagagc
     2281 ggtcccataa ctaaaaagga cgcacgcatg cggcggacca aatcatacgg tgccgtcgtc
     2341 gatgtcaact cccgggcatc aatgataagg gtatctcttt cggggtgttc tatatggacg
     2401 ccgagggatt cgatgacgtc gcagacggta tggacgtcag cgagcttcgg aatctcggtc
     2461 aatgtacttt tcgtcgttcc caacatagat gcgacaataa tgggcagtac ggcattttta
     2521 gccccactga cgcgaacagt gccctgtaag ggctttccac catgaataac aagtttctcc
     2581 aattctggtg cctcctaata gtattcgagt agttttatct aaatggcctc caaagacgcg
     2641 atgcctgaag atgccattct attgagcata taattactat caaattattc taccataaac
     2701 ggaaagaaaa aaaaaggctt ggcgcctagg cgacaagccc ttttttagga ataccttgta
     2761 aaggaaatgt tataggtgga tactccattt cgcttcttta ttgaagcggt ggaaggtatc
     2821 gataaaacga atcgttccgc tgcggtaacg catgacgacg gacatcgtcc gggcgccgcc
     2881 gccaaagtac tgaacgccgc gcatcatggg cgtattggta atggctgtcg ccgagaaaat
     2941 gcagtcatcc ccttttacca gatcatcgag ggtcatgacc ttgttaatat ctttgatgcc
     3001 catatccaaa cagcgctgcc gttcttcgtc ggtataagga atgaggcggg cctgcatatc
     3061 gccgccgagg cacttcaagg cggccgctgc cagaacccct tccggtgcac caccgattcc
     3121 catgacaacg tgaacgccgg aaccttcgat accggcgtcg accgccggtg aaacgtcgcc
     3181 gtcggtaatg agcttgatac gggcaccggc gctgcggcac tcgtcgataa ggtctttgtg
     3241 gcgcggccgg tcgaggatga cgacggtcaa gtcatcgatg cctcgttcta agccctcggc
     3301 gatgcggcgc aggttttccg gcaccgactg attcaagtcg atacgcccct tagcccgcgg
     3361 tccgacgcag agcttttcca tatacatgtc cggcgcgtga agtaaatcac ctttcccagc
     3421 aatagccatg accgaaatgg atccgtcctg cccttttgcc gtcaaattcg tcccttccaa
     3481 gggatcgacg gcaatatcga cttccgtacc gccggcaccg actttttcgc cgatatataa
     3541 catgggggcc tcatccattt ccccttcgcc aatgacgacc gtaccgtcga tgggaaccgt
     3601 cgacaagatc gaatgcatac cgtcgacagc cagttgatcg gcgccattct tatcgccctt
     3661 ccccatgaga cggccggctt ttaccgctgc cgcttccgta acgcggacaa actccaaggt
     3721 caatacgcga ttcataagga acctccttgt ctaaaaataa cgaaaacaca tatatagtat
     3781 tatttctagg caaagtaaaa aactcctgct tttagacagg agttttttta acagcatctc
     3841 agggacgaga cttttatttt tctttcgtaa tgacgtcgca gtgcatgtac ggacgaagga
     3901 cttccggtac tacgaccgag ccgtcagcct gctgatagtt ttcgagaata gcagcgaccg
     3961 tgcggcctac agccaagccc gaaccgttta aggtatgaac gaattccggc ttcgatttcg
     4021 ggttccggcg gaagcggatg ttagcccgcc gtgcctggaa atcttccgta ttggaacacg
     4081 aagaaatttc acggtatttc ccctgggccg ggaaccagac ttcgacgtcg aaagtcttgg
     4141 cagccgagaa accgatatcg cccgtgcaga ggcagacgac gtgataggga aggcccaaag
     4201 cctttaagat atcttcggca ttctgtgtca gtttatcgag ttcatcgtag ctcgtttccg
     4261 gagccgaata ttttaccatt tcgactttat ggaactgatg ctggcgaatg aggcctcgcg
     4321 tatcgcggcc ggcagaaccg gcttcagcgc ggaagcacgg tgtcagggac gtgaagtaaa
     4381 tcggcagttt actgccgtcg atgatttcac cgcggtaata gttggtaagc ggtacttctg
     4441 ccgtcgggat catatacatt tcttcgcctt cgacattgag cttatacatg tcttccgcaa
     4501 atttcggcag ctgacccgta ccggtcatgg aatcgcgatt gacgatatat ggcggaatga
     4561 cttcggtgta gccgtctttt tccgtatgtt ggtcgagcat gaaattgtac acagcgcgtt
     4621 ccaaacgggc gccggcgccg acatagtaat agaaacgggc accggtaacc tttgcggccc
     4681 gttcagagtc gagaaggcct aagcgttcgc cgacgtccca gtggttcaag ggttcaaagt
     4741 cgaactgttt cggttcgccc cacttgcgga cttccgggtt atcgctgtcg tcgttgccga
     4801 ccggaaccga cggatggcac atattgggga tcatgagctg gatggcgtgc atatcggctt
     4861 cgacagcctt gacttcctta tcgaggacgt cgatgtcgtc gccgagtttc ttcatagctg
     4921 caatctgggc gtcggcgttt tctttattgc gtttgagctg gctgatttcc ttggtaactg
     4981 tattgcgttc attcttcatg gcttcgactt tttgaaggat ttcacgccgt ttatcgtcta
     5041 aggcgacgaa ggcgtcgacg tcggcatggg cgtggcgatt cttaatattt tcctttactt
     5101 cttctacgtg gtcgcggatg aatttcgtat ctaacatgat agctgcttcc ttcctctgta
     5161 aatatacttt aactacctcg ctgcgatagt attataaccc tttttgttca ataccactga
     5221 acaaataccc catatgattc gtcgaattga gcaggaccaa acgccaggtc ccatcctgcc
     5281 attctaagac gttgatgcag gtattgtcct gcttgatctg ccaaaaatgg cttaaatcaa
     5341 ggcccagaac gtcacaaatg atggccttgt ttacggcatc gtgagctgcc accaagaccg
     5401 tcttgccttc atatttctga acgtaatcat cgaaggccgc ccggacacgg ctgcggacgt
     5461 cttccaagga ctcgccgccc ttgcccggca tggtgaccgt gtgaggcgc
//
LOCUS       DAWDXZ010000056         5450 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4204_length_5450_cov_9.517331, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000056 DAWDXZ010000000
VERSION     DAWDXZ010000056.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4204_length_5450_cov_9.517331"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1851..1860
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2299..2308
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3416..3425
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1605 a          985 c         1356 g         1474 t
ORIGIN      
        1 gcgggtactg gaagacagag tcagcgtacc aaaatccccg taaggggact gaaacgtttg
       61 ggcgttatgc ccgttcggat gccagtcgta gcgagtcagc gtaccaaaat ccccgaagtt
      121 aaatagactc cttttagaaa aaggagtttg tattataggg gactgaaact tatagtttgg
      181 gcgttacagt atgacgtttg gtcacagagt cgtattacca aaatccccgg agttacatgg
      241 actctttttt gaaaaaggag tttgtaccat aggggactga atctgacgct ctgatgtttc
      301 tacttttgtc gtagggtatt ggctgcatgc cttgcctgtt ggggagggat attcttacac
      361 cggccccata cctacttgga ctccttttag aaagggagtc atacaatagg ggactgagac
      421 gggtttgtcc gtaagccaac taatatgcct tccagcgtcg tattaccgtt ttcctcgcaa
      481 ggggtctgaa tgcacgacga aagacctatc gtcttttttg gatgataggt ctttgctttt
      541 tgatatgagg ggtactctat ccccgcataa aatatacttt tctgaaaaaa tttgatataa
      601 taaagacagc aattttgcga gctccggacc tgtgctgcaa cacggggcgg agctttttaa
      661 tttgcttaga tgagcggatt gttgctgcaa gtagaaagaa ataaaaaaga cggttgttgc
      721 atatgataca gcaaccgcta tcgataattg ttaatgaaaa tggacgaatc agaagactga
      781 aatttaatga gtatggagga atatatatgc cctcgttgga atggattgga aaagataaag
      841 ttgtaaatca tcatctggaa gtgccgtatc atgtgttgga gagacagtac agctatgatg
      901 aggatggaca gcaaacagaa gataatggca gtgaaaatat gattatccac ggcgataatt
      961 tattggcttt gaagtcgctg ctgcctaaat atgaggggcg catttcctgc atatacattg
     1021 atcctccgta caacacgggg aatgaaggct gggtttataa cgacaatgtc agggatcctc
     1081 gcatcgaaaa gtggctcggt gaagttgtag gcaaagaagg cgacgacctg tcgcgccatg
     1141 ataaatggct ctgcatgatg tatccgcggc tgaagctgct gcagcgattg ttggctgatg
     1201 atggaactat atttgttagt attgatgata tagaagctat gcatttaaaa ttgctgatgg
     1261 atgaaatttt tggtaaaaat aagtttatgg ctattttagt ttggaaaagt aggcaaaaca
     1321 aagacaatcg taatgtcact aatatttcta tcgatcatga atatgtttta tgctatggca
     1381 ctaaattaaa agggtgtaaa agagacagca atagatattc taatccggat aatgatccta
     1441 gaggtccttg gacaagtgcg aatatggtgg gactagcgac agaaaacgaa cgtcctaatt
     1501 tacattatga tttgattaat cctgaaacag gaattaatta tggaaagccg ttaaaaggtt
     1561 ggcgctatga ccgaaataca atggcgaaat taattgaaga aaataaaata ttgtggcctt
     1621 catctcctga tggaagacct agaaaaaagc aattcttttc ggagttatct gacagttata
     1681 cgggttattc atctttaatc ggaaaagatt gttataccag caatggtacg aaggaacaat
     1741 tagaaatttt tggttcttca atatttgctt ttccgaaaag caaattgctt gtatctgaat
     1801 tgttaaatca agctacagat aaagactcta ttattcttga cagttttgcc nnnnnnnnnn
     1861 ttatgataaa gttgaagggc caaaaatggt tgacggccat ttgaaggtca tcggtatcga
     1921 aagagcaaaa gagcatcaga aggggaccgg cggaaatttt agtttttatg aattagggga
     1981 accgctgttt catggagagt tgctcaatga agaagtcaca atcgatacga ttcgggagta
     2041 tatctatttc atggaaacgc ggcaatcttt gtcggaagaa aaggctgatg agccgtatta
     2101 tctcggttct tatgtaggga ctgcttatta tttctgctat gaaaaggatg aggcgacgac
     2161 actgaaccgt gaatttttgc ataccgtcaa gacgaaggcc gatcattatg tcatctatgc
     2221 tgaccgctgt acactgagtg atgctgagct ggcaaaattc cagataacgt ttaaaaagat
     2281 tcctcgtgat attgcacgnn nnnnnnnnac tttaggaggt ggcagcatgg aactgaaaac
     2341 atatcaaaaa caggtcatgg accatctcgc ggattacttg accagcttgc aggaaacaaa
     2401 tagtttaaag gaagcctgga aaaactattg ggagaaacaa gatattgccg ttggcgatgg
     2461 cggtgtcccg tcttacaatg atgccattga gggtgtccct catgtttgca tgaaagttcc
     2521 gacgggaggc ggtaagactt tcatggcttg cgcgtctttg aagtatcttt ttgataatct
     2581 gccgccggac cgcccgaaag tcgtggtttg gctcgtgcct tccaatgcta ttttagctca
     2641 gaccgtacgt aatttatcga cgcccggtca tccgtaccgg cagcggttgg aacgggattt
     2701 ccagaaccgt gtggaagttt gtacaaagga aatgctgctt tccgggcaaa atttttctcc
     2761 ggatacggta cgggaaatgt tgaccgtctg tgttttgagt tatgattctc tgcgcattaa
     2821 aagtaggaaa aaggatatcc gcaaggttta tcaggaaaat ggcaatctga atcgatttgc
     2881 tgtcgaactg ccgtatcgtg aggcgcgttt agacaatacg ccggatacgg cgctgatgca
     2941 ggttctgcgt caattgacac cggtaactat tgtcgatgaa agccataatg ccggatcgga
     3001 tttgagtaca gagatgctta ataatctaaa tccttctttt gtcctggatt tgacggcaac
     3061 gccgcgaaag aacagcaata tactggtata tgttgatgcc cgtgaactga aaaaggaaaa
     3121 tatggttaag ctgccggtta tcgtctacaa ccgtcagaac cgacagagtg tcattcaaga
     3181 tgccattcaa ctgagaggga atttggaaca gatggcaaaa tcggaagccg ctgccggagg
     3241 gcgctatgtg cggcctatcg tcctgtttca ggcacagccg aatattcgag aagacagcga
     3301 aacttttgaa aaactaaaag aaaaattgat cgatatcggt ataccggaag aagagattgc
     3361 cattaagacc gctgaggtcg atgaattagg aatggtagat ttgctttctc cagacnnnnn
     3421 nnnnnactgc cggattcgtt atatcataac catcaatgcc ttgaaggaag gttgggactg
     3481 cccctttgcc tatatcttgg cctctttagc caataagacg tctaaggttg atgtagaaca
     3541 gattttaggt cgtattttga ggcagcccta tgccgagaag aatgaacaaa atttactcaa
     3601 cttggcctat gtcttaacgt cgtcgaagga tttccactat acgttagaaa gtgttgtccg
     3661 aggattgaat gaagctggtt ttacccgcaa agattatcgc gtgggatccg ctgctccaat
     3721 atcggatatt agcatgccgg gatatgaaga aataagttta gacttagaga gtgagccggt
     3781 agaaacggta tcaagtgatg atagttcgga aagtacggaa gcggataatt tttcagatat
     3841 tgatagccgg caggtacagg tagggcttca gtcttctgca aatgattcgt ccgaagattc
     3901 gccgactttg caggatatga cttctcaggc ttctgaacaa gccgaacagt atgagagtga
     3961 attaaaggct tccgaggaat cttcgctaga aggtggtgag ttagggaata tgctgcatca
     4021 gtactccatt cagacaaact ggcaagatga aataaaacta ttaacgctgc ctcagttctg
     4081 cgtagaaacg gctcccgacc tgtttggcgg agcctatgca gtgttgaatc cggttcattt
     4141 attggaagga ttttcactca gacagcaaga tgcaacgatt aattttgcca tggctgaagg
     4201 tgacatgtat aaagttgata tcagtgacta cggggaagct gtgcctaaat atcaacggac
     4261 ccggcaaatg gagagcgact atcttcggga atatctggcg agggttccgg aagctaaaaa
     4321 agtaaatgag tgtacaaagt tactgtgtgg tcagctcaat aagtttaata attcatgctc
     4381 agcaggcgat attgcagctt atgtagggcg tgtcatcgaa cagatggatg cagatgaagt
     4441 cgcgaatatg gaaacagcac taccggtcta cgccaacaaa atccagacga aaatcaaaca
     4501 gctccaagcc gtttatcagg aaaagacctt ctatgaatgg ctggattccg gcaaaatcat
     4561 ttgtcaaagt aactatcaga tgccaaaaat cattacgccg gcagatgcta cagattctat
     4621 ccctaaatcg ctgtatgagg ccgaacgcaa tgacatgaac atttttgaaa ataaggtcat
     4681 cgatgccgtt gtcggattag ataatattaa atggtggcat cggatcattg accgtaagga
     4741 cttctgcatc aacggtttta tcaatcatta tccggatttc ctgctctgta cgatgaaggg
     4801 aaatattatc ctgctggaat cgaaaggaga ctatttagac ggtgatgaca gcaaaaccaa
     4861 gctaaaactc ggtcgtcgtt ggcagtctgt tgcaggcagt aaatatcgtt attttatggt
     4921 gttcgataag agagcgctgc agcaagaagg tgcctataca ttagaggaca tgctcggggt
     4981 attgcagtca ttgtaaaatt aaaggtcgta aaacgaaaaa atggcagccc tttaatctgg
     5041 gactgccgtt ttttttataa gataacggag gtcagtctgt ctagtcgctg cggtacgaaa
     5101 cttcaatcta cgccagaata gacattgatg gccgcaatgg ctggctgaaa gtttcaacca
     5161 agcggagcct gagagacctt gtatttgtcg atgagcttgt ggaagatggc agccttgttg
     5221 gggattcgga aattagatgg ggcggacggt ataagtgggc ttattgtaga caggtgtagt
     5281 accttcgctt ataatgagaa taagagaact gtagggggtg accgtgatga acagagcgat
     5341 gccgattggt gttgaagact ttaaaaaagt tcgcgagaac tattattttg tcgataagag
     5401 cgattttatc cgtcagctca tcgaccgcca cggagatgtc accttgatta
//
LOCUS       DAWDXZ010000057         5449 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4206_length_5449_cov_8.809974, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000057 DAWDXZ010000000
VERSION     DAWDXZ010000057.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4206_length_5449_cov_8.809974"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2033..2042
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1349 a         1391 c         1329 g         1370 t
ORIGIN      
        1 atggcctttt gcttttcttt cgacaggata ggaatgcgga ctttccgcag gtcaccgata
       61 gcgatgtttt ggatatggct gcctttagcc cagtaattca gttcactcaa gcccagagtg
      121 ctttgcaggt agagcatgag gtaggccggt tcaacggtat ccccatggca acgcaggata
      181 tagacgttgc ccgtcaccag cacctgttca tcttgaatgt cgtcggccaa ggccgtttta
      241 aagggcgcca ttttcgacaa gaggacgtcg cctggttgca agcaggccga ttctcctcgt
      301 tggggcaatt cttttaagta gggcaaatcg gtgcggatgc ggccgttttc aaggtcctgg
      361 accatcagat agcggtaagc cgtcggctcc ggactgacca attcgtccag gaggccggca
      421 ccggtcacga agccccgcga tacggtacag acgtcatcga cagtccatgc tttttccagg
      481 cggggatcag tcttggtcag gtaacgctga ggctccaaca cataccaatt ggccgccaat
      541 tgggccatgg agaggcggca gctctgttcc gtatccttct cgtaacacgt aaggatgctt
      601 tcgacatcct gatcggtcaa gacgttctgc cgtcgttcgg ccttgaaaaa attcgtcccg
      661 tcgaccatgc gaacgctctc gttgttttca ctgaggacca gcaggtaaaa gccgatggca
      721 ctgtttccga gcaggcgttc cggcaaggcg atgacccctt cgatccagcc gcggcgaagg
      781 aattcctgac ggacttcccg gtcgacatta ttatataagc ccttgccagg catcatgacg
      841 acggcccgcc cggacggggc gtgtctcata gcggctacga cggcaaagac ccattcggcc
      901 gaaatgctgc gcttttctcc gaaaaagaag gagctcaaag ccggataatt gcggacttga
      961 tcggccagat cccggtaccg cggccgcaag ggaacgcggg taaagatttt attcggctct
     1021 ttccccctgg cgatggtcgc cggttccagt acatcgccgc aaatatgagt aatcggaagt
     1081 tcggccaaga ccgtgcgcag gacggcgccg acataggatt tcttatcctt ttctacagcc
     1141 gtacaggtaa tccccggaaa ggccttgcat gaggccaagg caaaatcgcc caagccggaa
     1201 caaagatcgg ccagtttgtc gccctcttgc agctgcagaa gacgtccggc caaggaggtc
     1261 acgccggccg gtgtttccgt agccgaaagg gaccgataga acggcaggga cgccaagatg
     1321 tcggccacat cgttttcacg aagactttct gccgcttcga agaggatcga actgtagcgg
     1381 gaaaagattc ccttcaaata agtccgtacc gtcccatccc aaggaccttc gtccaaatag
     1441 gccagcaatt catcggccga ctgccgttgc tgaagccata agacgagagc gaaaaaggcc
     1501 aactcgtcaa tgccgtcatg ccgctctagg tcggcctgca tcatctcttt gacaatatag
     1561 cgatattccc taactcgtct catcacacat cccccctttt caaatagacc ggatggacgc
     1621 cctgcaaggg acgcatgtcg gcttcataaa tcgtcaactg acggaacacc ggccgagcca
     1681 actcccggat ggccgcccaa tctgccggca cgccttcgtc cagatcgaag tccaaatacg
     1741 aacgaggctc taacgactcg tacatgcggg tgtaacccgg tacatcttca aagacctggg
     1801 gataaaaagc catggccgca tcgaggatca tatccggatc attcggaccc ttggggacga
     1861 tgacgtcaac ccgctgtccc cgacgcttaa agccacgagc cacttcaaac cacatgcgac
     1921 tggaaacgcc gcgccacaac tcaagctcaa taccattgat tccgtcgtaa cctgcatgac
     1981 caaagaatac acacttatca aaaccagcca taattatcgt cctccttgta ttnnnnnnnn
     2041 nnttgtatta gaataactat tttgtatttg tttaatcata gtataacata tttttctata
     2101 ttgtcaaatg gtttttaata accttttata aaagtagtaa aaaaacaagc agtcaaaaga
     2161 ctgcttatct tcttaatctg aaaacagaac ggattgctcg gcaatctttt gtcgttcgca
     2221 tggaaaccat gaaaacgcgg ccgaagttgg atacgttgat caaaattata caggctctcg
     2281 atttaaaatt aaaattaact gtcactgctt tataatgcaa aaccaacaaa tcccttgaac
     2341 cggtattccg attcaaggga ttttctcttt ctgtacaaga tgaaaaaaac accgtcacag
     2401 tttcggctgc ggcggtgtat ctctatactt gccttcatcc ctaatccgat tcctcttcgg
     2461 gcaaggcctg ttcatcttct tcccgactga agaaacccgg agcgtcttct gcgcggtggc
     2521 ggatgggaat catgaaaatc cacagtttct cgtcgccttc ggcgaagcgt tccagctcgt
     2581 tcttagcgac gtagatgagg tcctccgtag gccattcggg atggggcagg attttcaaga
     2641 gggcttcccg catcgcttcc cgggtccagt aactgatgta atagccgtac tggtcttccc
     2701 gttcccgata atcggctgtt ttcgtcagga agtcgaagat gccttgagga atattttttt
     2761 taattttggc gaagataacg tagtcccaag ggaaatattt ccggaagacg tcattggtcc
     2821 agattccttc cagacgcatt tcctcggcgt agtagtaata tcggctgttc ggagcccatt
     2881 cactcaaggc gtcattccag agactgttga ccggcatata ggacgattcg gcctcgaagg
     2941 ataagaccag ttgtccatct ccgaggctct tgtacgattc ttctgtaatt ttgcctcgga
     3001 tggccccgcg ccaaccgtat tcacagtcgg gatacaggag gtctatgatg ttgttaaggt
     3061 gctgttcgta aaattcccgg tcgacgaagc gtttcgtgtc ttcatcggct tcgtcatagg
     3121 aatggaggga aaaggcctgc agcaggtggt ttccgatatt ccggatttcc tcttccggcc
     3181 cttcgaagcg atacttagtg taacaaatat tggccatgat gttgtctcct ttcagcgaag
     3241 tttccccttt tggcgaagtt cttccaaaac gggaatgaaa tagtgatgac gttcgcgcac
     3301 cagataatgg atccggcgga tgatagcccg cgttccaaag ctcccattcc ccagcggaag
     3361 gccaatcgtc gaagccacga cgtcggctaa gtcatcggga gagagattag gcagcggcac
     3421 cagagccagc agctcccggt acagttcctc ccgtggccat tgggagtaat agacgtcgtg
     3481 gtcccggcta aaaatattgg aatcggagta ttgataaagg gtatggcgag tgaaaattcg
     3541 agcgagctct tccggcgtct cttttgaaag actggcccgg acggcataat cgcctgaaaa
     3601 ataatgatgc tccacatcat tggattcggc atcgccatcc cggagatcgc cataggccaa
     3661 ataatagtag cagctgttgg gcgcccactt acggagcatg agattccaaa ggtcgcggta
     3721 gttgccgccg tcaacgttca cggccatgga ccacttgccg tttggcagct gccggcaagc
     3781 gtcggatctg atgtcgccgc agacagagcc attgtgccag atgtccttcg atccctcaag
     3841 ggcattgagg atatggacca ggtcatgact gccgtcatcc cgcacaaagc caagggctgc
     3901 gagctcctta tcccgttcac tgggcggatg tgattgactt ttgaaaatgc cgttgagacg
     3961 ggtccgcagc gagaaaagct cctcctcggg accttcgaaa taataatggg taaacatagg
     4021 catacctcct gtcggaaaag acaagaatgg caaaaagtac gcaaaaaagg cgcacgtata
     4081 gaaccattct ttttaatagt tctaaaacat gcgcctaaac agtcaatcat tcggcttcga
     4141 ttagtatatc ttgtttgcgt caaaaagtca agccataacg gaactctcga tattatgttg
     4201 aattctatta acggcaaaat cgctccatta tacattaata aacagcaata caacaacctc
     4261 cggacatagg aatcagctcc tacatccgga ggttgtttca cttcctgctt actaatcgtc
     4321 ataataatcg cactggcgag aatccttggc taatcaccta ggaagtttca gtcccctatg
     4381 gtacaaactc cttttttcta aaaggagtca atttagctcc ggggattggg gtaatacgac
     4441 acaataaaga cgttgaggta accgtttcct caactgggga gtttcagtcc ccttacgggg
     4501 attcgggtaa tacgacgaga ggttctgtgc tacgattgct accgtgagct gaacacgttt
     4561 cagtcccctt acggggattt gggtaatacg acagcgcagc tcactcctga ggtattcaag
     4621 cagtcatacc tgtttcagtc cccttacggg gatttgggta atacgacctt tgaaaagttc
     4681 gctgtggtaa gagacaccat tcttaagttt cagtcccctt acggggattt gggtaatacg
     4741 actaaacgcg tatcccagca atgcgataac gcgcatcgat ggtttcagtc cccttacggg
     4801 gatttgggta atacgacctg aaaatgcctt tttctttaag gcattcagct gttgtttcag
     4861 tccccttacg gggatttggg taatacgaca aaagaaagat tataggactc ggtttaacgt
     4921 ctcgagagtt tcagtcccct tacggggatt tgggtaatac gacaaaagaa agattatagg
     4981 actcggttta acgtctcgag agtttcagtc cccttacggg gatttgggta atacgacatc
     5041 agccgaacag ttgtcggcgt tcaagtatcg ccgtgtttca gtccccttac ggggatttgg
     5101 gtaatacgac acccattcta tctaccattt ctcttggaga aatatagttt cagtcccctt
     5161 acggggattt gggtaatacg actcttctaa tgagtttctt gatcgtagct ataatatacg
     5221 tttcagtccc cttacgggga tttgggtaat acgacactgt ctctcagtac ccgctctggg
     5281 agcgggctca caggcgattt tttcaaacct ccggcttttt tcctcttttc gagggacccg
     5341 ggtgaaaaat aaaaatcggg cagtgtcgtt ttttccctta gttacgccat ttatagccgt
     5401 ttttgaaatt ttcaaacctc ccggggtttt cgccccaaaa tcggttttc
//
LOCUS       DAWDXZ010000058         5436 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4216_length_5436_cov_8.963576, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000058 DAWDXZ010000000
VERSION     DAWDXZ010000058.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4216_length_5436_cov_8.963576"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1198..1207
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1713..1722
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2627..2636
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1211 a         1398 c         1403 g         1394 t
ORIGIN      
        1 cctttcatga agggcaggta tttctgatga agggcagcgg cctttttgac gtcgccgtct
       61 ttataggcct gaatcatggc atccatttcc ttaccgatga cgtggccggc aacggtgatg
      121 acaccttcgc cgccgacggc aacgataggc agggtcaaat tgtcttcgcc gctgtaaagg
      181 tggaagcctt ccggagcatt ggcagcgacg taaccggcga agtcgaggct ggcgctggcg
      241 tctttgaggc cgatgatgtt gtcatagtca cgagctagtc ggcagaccgt ttccggttcg
      301 atactgccgc cggtacggcc cggtacgttg tagatgaaaa acggcaggga cgtcgccttg
      361 gcaatggctg caaagtgggc gtaacagccg tcctggttcg gtttattgta atacggtacg
      421 atgcacagca agagatcgac accggtggct tcggcctttt tagcgatggc aatggactca
      481 gccgtattat tggtaccgac attgcccatg ataagggtct tgcctttcaa ttcgtcggca
      541 acgactttaa aaagcttgag cttttcttct tcggtcatga cggctccttc accggtcgta
      601 gcaccaagga ggatgcccga accttcgtcg gcatacttct tagccagttc gacagcggct
      661 tcataattga cggaaccgtc ctgattgaaa ggcgttatca tagcaatgat gatattcgga
      721 aaggttaaca taaagattca tctcccatcg tgatctcagt gaatcatatc gtgatccagc
      781 atatattcgg caatctgcag ggcgttgagg gctgcaccct tgcggatctg gtcgccggaa
      841 acccagaggt tgaccgcctt cggattgtat aaatctttgc gaatacggcc gacgtagcag
      901 tcgtattttt ccgacgtaaa taaaggcata ggatattcca tcgctgccgg atcgtcctga
      961 acctgagcgc ctgggaaggc atcgatggct ttacgaacgg catcgacggt caattcatca
     1021 gccgtttcta cgtaaatcga ttcggcatgg ctgcggaaaa caggcacgcg gactgtcgtc
     1081 gccgtaatgg caatagagtc gtcatgaagc attttgtgcg tttcgttgac catcttcatt
     1141 tcttccttgg tataatcgtc ttctacaaac ttatcaattt gcggaatgag attaaagnnn
     1201 nnnnnnnttg ctgggcaaga ttttcggttc catctcccga ccggctaaga aagcttccgt
     1261 ttcttcgcgc agttcgtcga gcccttcttt cccggcaccg gacacagcct gataggtgct
     1321 gacgatgatc cgtttgatgg gcgacagatc atgaatcggt ttcaaagcca tgagcatgat
     1381 aatcgtcgaa cagttcggat tggcaatgat gcccttatga agggcgatat cgtccggatt
     1441 gacttcggga acgaccaagg ggacgtcggg atccatgcgg aaagtgctgg agttgtcaat
     1501 gacgacgcag ccgcgtttcg ccgcttccgg tcccagttct ttactgaccg agccgccggc
     1561 aaagagtccg atatctaccc catcgaagga ttccggcttg gcttcttcaa cggtatattc
     1621 cttgccattg accgtcaatt tcttgccggc cgaacggtgg gatgccaaca acttcaattg
     1681 gctgtacggg aattttcgtt cttcaatcag gcnnnnnnnn nnccggcaac agaagcaccg
     1741 cgggccccgt ccaggctttc atgggtcttg tccggagctg ccttaacacc ggcagctttc
     1801 cgcgcttcat cgattttatc agccgtcatg atggccgtcc ccgacggagc gtcgagttta
     1861 ttgttgtggt ggagctcgat gatttcaacg tccggcagat agggcgcgac ttccgtcgac
     1921 aacttcatca tcaaaacggc gccgatggaa aagttcggtg caatgaagat actggtcttc
     1981 ttttccaaag ccagggcatg gagctcatga cgctgttcgg cagtaagtcc cgtcgtaccg
     2041 acgaccatgt tgatgccgtg agctatgcag gttttagcat ttttaaaaat gacggcaggc
     2101 gtcgtaaaat cgacgacgac gtccggctta atatcgtcaa gggccttggc aaaatcgcct
     2161 tcgatggcaa ggcctaaatg accgatacct gcgacttcgc cgacgtcttc cccgacgtgg
     2221 tcgatactga caccgccggc gacggtcagt tccggatctt caaagacggc tttgacgacc
     2281 tggcttccca tgcggccgtc tgccccattt actaatacac gaagcatatt gattccccct
     2341 gtagttttat gatttgatag cgtattttta ttttaaaatt atctttaact atatacctat
     2401 cgcggacatt cgtcaacctg tatctgtcac ttcgaaggct gccttacgat cccttcacct
     2461 gttgtacgat ctgtctggcc gcttcaatac tggcttttgt cgtatcgttg ccggcaaaca
     2521 tgcgggcgat ttcaaggacg tgttcaccgt cagaaaggct gcgtacctgg gtgaccgtcc
     2581 gaccgtcggc ttccttctta tagatgaaga actggtggtc ggcaatnnnn nnnnnnatgg
     2641 ccgccgtctg cggcaagtgg gtgatacaga aaatctgtcc tcggccggcg agtttccgga
     2701 tatggtcggc gacctgaagg cccgtctgac cgctgatgcc gacgtcaatt tcatcgaaaa
     2761 tcatggtctt atcggcatcc tggccggacc cggtcgtctt caaggccaag gcaatacgcg
     2821 agacttcacc gccggaggca atttttgcca agggctgcat agcttccccc gtattggccg
     2881 aaaaataaag ttcaatgccg gaagcaccgt ccggtgaaat ttccttcata ggctctatgt
     2941 ggaaggcaat gcggctattg ggcataccta gcttgtttaa ggtctcagtc ataaccttgc
     3001 tgaaccgggc atcggcttta tggcgcaaat ccagtaaaac ccctgcctga tgccgtacag
     3061 cctccgcttc tttcgccagc tgcgcctcga gttttgccaa gtggctttcc gactggtcaa
     3121 ggcgttcata gtcttccttg gctttggcca aaaactgcaa aatgtcagcc acggtgacgc
     3181 cgtatttgcg ctttaatttt tcaatggctg ccatgcgaga ctgcatggcg tccaaagcgt
     3241 gctcgtcgaa gtcgaaggaa tcggcatagc gaagaagagc atcgtggata tcttcgattt
     3301 cataggacaa ggtttctacc tttcctgaca aggcactgaa ggtatcgtcg taggtcgagg
     3361 ccttctcgag gctgcggtga atcgtctcta actgttcgat agccccggtc tgacgctcgc
     3421 cgccttctaa cgcaaagatg gcatcccgca gattatcctt caggtgttcc gcatgggacg
     3481 ccttattgat ctttccttca agctcatcgt cttcgccttc ctttaactcc gccccttcga
     3541 tttctttgat ttgaaaggcc aggatactga gcatccgagc cttttcgcct tcatcgcggc
     3601 gcagggcctt gatggccctt tccgttttat gccacgtgac atagagctcg tcatacgttt
     3661 ctctggcctt acggacgtcg tcattgaggc cgtctaaaat ggcgacgtga taggcaggat
     3721 taaagatgag gctgttgtcg aactggccgt gaatatcgag aagataatcg cctacgcggc
     3781 gcaggaccgc cgtcggcacc aaggtgccgt taaccagaat ggtccccctt ccgctgcgct
     3841 ggaactgccg ggaaatgatg agctgtccct cgtcaacttc gatgtgatta tctgccaaaa
     3901 gggctgccaa tttggcaggc atatcggcaa aaaagaacga gccttcgacg agaaagccct
     3961 cggtgccgtg gcggacgaag gaggccgacg cccgttttcc ggccaacata ccgatggcgt
     4021 cgaggagaat cgatttgccg gcccctgttt cgccggtaaa aatcgtcact ccgtcctcta
     4081 agtccaaatg caggtcttca atcagcgcaa agttatgaat gtgcagggac tgcagcataa
     4141 gaacctcatt ccttcatcaa cgatacgatc aatttaatca atttaggaac tgtttctgtg
     4201 tctttcacaa tgagcaggac cgtgtcgtcg ccggctaagg taccgatgac gttttcccag
     4261 cgggcatggt ccatagcagc ggcaatatgg ttggccgatc ccggcagggt atggacgatg
     4321 atctgattgt aagccgaatc gacgcggacg atggagtcct ggaagagacg ggccatgcgg
     4381 ttcttggaca tgagcatgtt ctgttccggc gacaaagcat agcgataatg gccgtcactg
     4441 gtcgggacct tgatcagcat cagttctttg atatcacggg acaccgtcgc ctgggtgact
     4501 tcaatgcctt cttcctgcag tgccttggct aattcctcct gtgtttcaat cggtctggac
     4561 tggacaatct ctttaatctt tgtgtagcga aatcttttca taatgacctt tccctctctt
     4621 ctgtacgaca tataataagg gcggacagtg gacctcattg atgggtttcc acgacgagac
     4681 gtgaaaggcc ccttggggta cgtccttcaa aaatgattgc agcgctcttt cttcctgagc
     4741 gccggcctca gtaccgggat aagctgctat tataattatc ccatttatcg caattttatt
     4801 caagcctatt tttagagccc ttagtgttat ttcataatcc gtatggatat gatggtctcc
     4861 ggacggcaaa tagcccaaat tgaataccat gacatcgatg ccgtcaggga ccgtttccaa
     4921 gagttcgtca tgagagccac actggcagcg gacatcgcgg tcggtcagct tttcttccag
     4981 aagccgctgt ttcgtccgtt caacggcaca gggctgtata tcaaaggcat agaggacggc
     5041 atcggccggc atgagttttg ccaaaaaagc cgtatcatat ccgttcccac aggtcatatc
     5101 gacaatcact ccggcccggt ggacataggg atcgaggagc atgtgcgata ccgtaacggc
     5161 attgcggaat ggatacatat caatcatttc ccatcatctt agtaaataaa ttactataaa
     5221 aataattttg gttaaaacga atcagtttca gtacgtgatc ggcttttcgg atttcaaggg
     5281 tcatattgcc catcaattcc tgatccatca cgccgtcaac ggagacgcgg acgctgttgc
     5341 ggctgtgggc cgacgtgact gaaatctgat cgtcgtcgcg caggaccagg ggtactttca
     5401 ggagcagatg cggacagatt ggcgttacca tgatgc
//
LOCUS       DAWDXZ010000059         5339 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4267_length_5339_cov_11.031226, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000059 DAWDXZ010000000
VERSION     DAWDXZ010000059.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4267_length_5339_cov_11.031226"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4789..4798
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1241 a         1285 c         1521 g         1282 t
ORIGIN      
        1 atcccttatt gaagaaggat taaaccggat atatgaagcg gtccgcaagc attccggctc
       61 cgtaccgtcg gctgattgat gacacctatc ttttgttgag gcactgtggg cagtgaactg
      121 atttttttga tatatgaggt gtttattttg aacataagaa cagcaacatt aggagattta
      181 caggccgtta cggccgttgg agccaattgc tttccgcctg cggaagcgca gcgacagacg
      241 tcgaatttaa agaccgtctg acccattatg gcaatcattt ttagctgctt ttcgatgaag
      301 cggacctgac ggacgatatg tatgccgatg cgtctcttca caacgaccag ggggcctggc
      361 aggtgatctt tggcgtcaaa tacgatttcg tcctgccgcc gtcagggcta tgtcggaaaa
      421 ctgatgcagg ccgccattga tgctgcccgg catcagatgc gtcttacttt ctaatcgaaa
      481 atagaaccta gagaggctgt ttcatgcgat aaaaatcttg catgggacag cctttttgga
      541 tgcttaaatg gtatataaaa taacatatga acacttgaac atatgttatt ttaatggtat
      601 cattaggcat ataaagaatg tcttttaaaa gggggctgcg ttatggcaga gcatacgtta
      661 catgaagaaa actgcacttg ctgcggtcat catcaccatg atgacaaata cgttgaaaaa
      721 gaagaacact gcagttgctg cgagggtgag gaatcggtca gtcttgattc tatcggtacc
      781 tgcagctgct gccacaatga agaagtagaa gaggaagaag ggggcgcctt gtggaccctg
      841 gtcgtcggaa cccttgtctt tttaattacc tatgtaaccg acctcgttcc cgaagctttt
      901 tcacttcccg cttatatcgt cgcttattta ctcctcggct atagtgtcct tcgcgaagcg
      961 gctgaaaata tcatgaagcg tcagcctttt gacgaaaatt tcctcatggc cgtagctacc
     1021 atcggggcct tcgccatcgg tgattatccg gaagccgttg ccgtcatgct cttcagccgt
     1081 atcggggaag ctatggaaga tcgcgctgta aaccgcagcc gtaaacaggt ggcggctgct
     1141 atcgatatgc ggcctgacgt catccaccgc cttatggaag acggaagcac cgttgaagtt
     1201 ccggctcgtg atgctgctgt aggcgacatt gccgtcgtcc gcgtcggcga ccgcatcccc
     1261 ttggacggtg tcgtcgtctc cggcgacagt cttctcgata cgtcggctct gaccggggaa
     1321 ccggttcccg ttacctgcca tgaagggact gaagtcatgt cgggcagcat caatacgacg
     1381 ggcgtcctcc acgtcaaggt taccaagcca ctggcggaat cgatggtcac taaaatcttg
     1441 gaatccgtcg aaaaagcggc ggccagaaaa cccaagatcg accgctttat cactcgcttt
     1501 tcccgcattt atacgccggc tgtcgtcgcg atcgccgcgg ctacggctat catcccgtcc
     1561 ttgattacgg gcgaatggca ttactggatt tatacggctt tgaccttcct tgtcatcagc
     1621 tgcccctgcg ccttggtact cagcgtgccc ctcagcttct ttgccggcat cggtgccgcg
     1681 tcgaagcagg gcatcctgtt taaaggcggt attgccgtcg aacagcttaa aaatatcggc
     1741 gctgtcgtct ttgataagac cgggacgatt accaaaggaa acttcgtcgt ccaccaggtc
     1801 ctgccggccg atggctttga cgaagaagag gtgttccgcc tcagtgcatc ggtggaacag
     1861 gcatcgaacc accccattgc ccgcagcgtc gtcgaagcgt ctgaaaaacg aggcttgacc
     1921 ttggcacaac cggaagccgt tgaagaattt gccggccagg gtctgaaggc cagcgttgac
     1981 ggtcacgtcg tcctctgcgg caatcagaag ctcatggacg gtcatgccgt ggttatgccg
     2041 acgatagaag cggctgaata cggtgccgaa ctgttcgttg ccgtcgatgg ccgctatgcc
     2101 ggccgtatgg tcgtgtccga cacgatcaaa gaagatgccg tcgatgccat taaggctatt
     2161 tccggtctgg gcattacgac ggccatgctt accggtgatt ctcagcaggc tgccgattac
     2221 atcgcctcca agaccggtat cgacgacgtc cgggccaagc tcttgcctca ggataaggta
     2281 cgacaaatgg atgaacttcg taagaaatac ggtccggtta tgttcgtcgg cgacggcatc
     2341 aacgatgcgc cggtcctggc cggggccgat gtcggggcag ctatgaacag cggtgccgat
     2401 gcggccgtag aagcagctga cgtcgtcttc atgcattcca aagtcagcgc cgttcctaag
     2461 gcccttcatc tgtcgaaaga aacgatgaag attgccaagc aaaatgtagt ctttgcctta
     2521 ggcgtcaaag ggctggtcat ggtcgccggc tttgccggtt acgcctccat gtgggctgcg
     2581 gtattcgccg actccggcgt cgctgccctg tgtgtcctca attcggtccg catgctctat
     2641 aaaaaataac tgaccatctc ccgatgacag ttccataaca gaaagccccg tcgaagaaat
     2701 ttccttcgac ggggctttgt tgtagatgct gcttgattgc tatttcgtaa actgcagggg
     2761 gactgtcgat acgacgacgt ctaagtaagg cagggtacgg agtcttagca gccagccgct
     2821 ctggttgtga agcaggcggt gccataattt tttcgtttca aattccggga tgaggacggt
     2881 aatcgtatct ttgggagccg tctctttccg cagctcttgg ataaaggtga tgagcgggtc
     2941 tgtcaactgc cgatagggag actggagcat gatgagctcg atgtcaggtt ccaactgctg
     3001 ccacaaggtt ttgatctttt tgccccgttc atcgtcgctg tagacgttga cggcatagat
     3061 tttatctttc gaaccgctga tgatcttggc gtagcgaatg gctttggcta cggcttgagt
     3121 cggtgaagca atagggatga tgatgatatt gcggcctaag tcggtggtca tgaccttcct
     3181 gtattcctcc ggtgtcagca acagctgttc ccggacgtca tcatagtgat gatggacttt
     3241 aaggaacata tagaccaagg tcgggatgag caggacgatg atccaggcac cgtagatgaa
     3301 tttggtgatg aggacgatga cgacgacgca ggccgtgacc agggcaccga agccgttgat
     3361 gacggaaaag aggcgccagt ttcgttcccg gcattgtctc cagtggacga ccatggccga
     3421 ttgggcaatg gtgaaggaga ggaatacgcc gatggcatag agggaaatga ggtgctcaat
     3481 attaccctgg aagaggacga tcaagatgcc ggccatgagc gtcaggactt ggatgccgtt
     3541 agaatagctc aggcgttcac cgcggttggc aaagtagcgg gggacgtagc cgtcgacggc
     3601 catgaaagaa aggagcggcg gcaggccgtt ataggccgta ttggcggcga tgtagaggat
     3661 gaccatcatc aaaatctgga tgacgtagta aatcaaggtc cggctgaaga tatcttcgac
     3721 aaggagagac aacagggtat agccttgtgt cggtaagagg tgaagataaa tgatgaggta
     3781 accgaggcct ccgagcatga ttgccaggat acaggccatg acagccgtcg tttcgatggc
     3841 gtttttttgc tgaggcgtct tgaacatggt gacgccgttg gcaatggctt cgatgccggt
     3901 catagaacta cagccgttgg caaaggcccg caggacgatg agcatcaggg cggcctgatt
     3961 caatggggct accgtttcga cagcgacggt atgagccgtt agggtgccgt tccagatttg
     4021 atagaagccg ccgattaagg tgatccccat ggcagccaag aagaagtacg tcggccagac
     4081 gaagacggtt gaggcctccc tggtaccgcg caggttgacg atcatcatca ggatgagaac
     4141 gccgaggtcg agatagacgt gataagggtg gaggaccgga aaggcactga ccagcgccgc
     4201 cgtccctgaa gaaatggaaa cggcaacggt caggatgtaa tcggccagaa gcgccgctcc
     4261 tgccgtgagg gccgggtaga caccgaggta tttcatggcg atgccgtaag aaccgccgcc
     4321 gcccggattg acccggacga cttgggtata ggacagggca acgatgaata ataaggcaat
     4381 gatagctaag acgataggtc cgaaataacg gtatgtcgct aaggtcggta ccgaagccag
     4441 gacgatggca atctgttccg gcccgtaccc gacggatgac agggcatccg atgagaaaat
     4501 aggcagggcc ttccattttg gcatctgttc gccctcgagt ttggtattct ccaaaggctt
     4561 gccgagaagg tggcgactaa tcgttttgaa tagcatgata gcctcctttt tttattgtaa
     4621 gaaactgcat tttgctaaat atagttcagt ataatcccac tttattatcc tgtcaactga
     4681 gattttaccc ttatcattct cgttgggaat gaggcattga aacccagcgt aaaaaagaca
     4741 tcctttgacg caagggaaag tcaaaggatg tctttttcgt aaggaggann nnnnnnnnga
     4801 ggctacgaga gcttctaatt tagcaagtct tgcattgaga tcctgattct ggctttccag
     4861 ggcttcaatt ttttgattct ggtcagtcag ttctttatcc tgcgattcca atttaacggc
     4921 catttccgac ttgctcatgt gagcgtaagg ggaagaagag ccgattttga aggaaacgcc
     4981 ggcgctgtac atgttgtcgt tgttgccgat ggtagcggca aggtttacca tggtgtcttc
     5041 attgggacga tagaaggcgc cgagggcggc agcgtgttcg ctcttgtagg agccgaatcc
     5101 gacggcgaag gagcccttgt catcggggtc gaaatctaaa ggatggaggg atgccaaggc
     5161 agcggcaccg gcaccgacgc ggttgacgtc gcggccaatc tggttgatgc ggccgttcat
     5221 ttcgttcatg ccgttaccga tgttgttgat cgtccggtcg aactggcctt tattgatggc
     5281 atcaccggct tcggttgctt cgccgacgtt gtgaatggca ttgccgccca tgttgatgt
//
LOCUS       DAWDXZ010000060         5333 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4271_length_5333_cov_6.279651, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000060 DAWDXZ010000000
VERSION     DAWDXZ010000060.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4271_length_5333_cov_6.279651"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1984..1993
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2436..2445
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4486..4568
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1473 a         1338 c         1021 g         1398 t
ORIGIN      
        1 tatctcaact cctttatagt tacacaattg ccggagtatc tcgcccagat cacgtctata
       61 ttgattgaaa ataatttttc gtctatactt aggtgtgaag acgatatgat atttgcacaa
      121 ccatttcgta tgtgctaagt cattggcttt cttcgccata tgaatcacct ttccttttta
      181 tgtaatagtg gcttggacac ctctattata cggaaaggtg atttttttgt ataactattg
      241 gaacacaccc gcatagcggg tagtttattg tttcgcaggc gtggcctgct caacagggta
      301 aaccccatat ataaaagaac aataaccacg cagtcttcta actgcgtggt tattgccttg
      361 gcaatactat ggtgaaggcg gtcaaccaaa gatctgaact catcgataac tgccttttat
      421 ttaattacac ctgcgtctga atgattccgc agttcccagg caatacggac actctcttcc
      481 aattcttcca gcccataaaa agcggccaat atatcttttc caggaacgat gggtccatga
      541 ttagctaaaa gaaaggcatc actgcaagaa atacaagctc gaaaagcagc aaaaagctct
      601 tcactccccg gttcagcata aggaactaag ccaatggtcc ctaccttcat cgtaagatat
      661 ggcgtatagc gcggcacaat atcctgcacg ttatcatgtt gaatacaaga ccacaaggtc
      721 ccataaaaac tatgggtatg caaaacagct ccgatttcct tatctttttg ataaaaagct
      781 tgatgaagag gaaattcctt actgggtttc cgtccctcta taagggttcc gtccatatca
      841 agttgggtaa agtcagtcgg cttaagccgc ccgaaacagg ttcctgaact cgttatataa
      901 atttgattgt tatggcgaac actcatatta gccgatgatc ccgttgcttt atttcgttta
      961 aacaaagatc gcgctaccca gcacgcatct tctaatttct gttttaaaat cttatccatt
     1021 catatctctc ctgtcatcgt taaagcccga ccaaaaaatt cttcttgccc aaaatttccg
     1081 gacttcaaga caattctcat ttcagggttt tccaatggtg ccataacagg aactccgggg
     1141 gcaatacttt gaccaatcca ataggcttga tatcctaacg ccttagtaac ggctccagaa
     1201 gtctcgcctc cggcaacaat gatatgccgt accccttctt ctttagcaat aacggctgca
     1261 gctgctagca tgttttcaat gagcaatgaa taggtcagca gagtttctcc tcgaatttcc
     1321 tccagatatt gcggcgtttc tgaactgtaa ataaggacat cctggtcttt gtgagcccgc
     1381 aggaattgtc gaatatcgtc tagcccctgt tccttcttca aaatcttatc cggataaagt
     1441 ttataggaca aaccgccccg ttcctgaaac caagctattt gctgcagcgt agcctttgaa
     1501 cagcttccgg ccagcaaaac gcctcgtcct tcacttccgc gaagagcttt ccgatcatac
     1561 cttttatgtt gcagcaaata ttcggcccaa gccgtaagta tacccgaccc tccggtcaac
     1621 aatttcatcg cgccaaacga cgaaataatt ttttcaccat cttcatcggt ttcataatcg
     1681 ggaataaaat atccccggcc tttcaactga ctgtgcgccg cgagttcgat atccttccac
     1741 gaaagatgcc gactctcata tcgactctgc ggacccataa gacggataag attactgtcc
     1801 ctcatgggcg tcaaaggatg attacgcata tgactttctt caagtttaac accatgaacg
     1861 taaagaacag catcttttac cgtccgccca ttaaccggga gcgacgggca taagagggta
     1921 ttttctacct gtaacctttc catcacagca tcacaaatag gtccgatgtt ccccttgggc
     1981 gttnnnnnnn nnnacataaa aatgcttggc tccttgtttt tccagccagt caatcgcgtc
     2041 taatgaatcc accacggcag aatctctttc ctgcgtcctt gttttcaagg caattacaac
     2101 ggcatctgta tcggttcctg tgatatcgtc ttcagggatt ccgttaaaca gtaacgttcg
     2161 catccctcct tttaccaaaa aggaagctgc atcactggct cccgtaaaat catctgcgat
     2221 acaacctaga atcatccttc ttcactccca ttcgttcaat cagtcgatat agtatcatta
     2281 ttttttcgat aaaattcttt gaatcaaagt tttggcacta atagtagggc tggtaacgat
     2341 ttcagtgtct gttgtaaatc cgccctgtaa tgcccgcacc atagaaattt gtgcaaaaac
     2401 aatcttgtcc accttaccgt caaagttccg ggcagnnnnn nnnnngcctc ttggataagt
     2461 ttattatgtt tcgcttcatc cccgtcattc aaggcggtaa aagctgccgg aatcacagca
     2521 acctcgacct gaatatcctt ttcctgctgc gaagccatct caagcaaaag gcgtttagtc
     2581 gtaggtccgg ccgcaggaac tgtcgcaatc actccgacac gagaagcctt tttgactaca
     2641 taggccaaca tagccaggtc agcgctgatt aagggaacgt cgacatatgt ctgcagttct
     2701 tcagcatatg gactcatcaa cgaacagttg agcatgatac cttgagcgcc tgctgcttct
     2761 gcattccatg ccatctgtaa gatctcacgt cttacccgag gcgttgcccc cttcgatcga
     2821 agttccggca tcaccccttc atttaaaaag ttaatgcatt caatctcctt tcccagcgtt
     2881 tgaaatgcgg aattactggg tccgatagaa ttcatcgtcg tatgaattaa tcctattttc
     2941 atatattgcc tctctattca ttgagccatc ccctttcaat gaatcgtcaa gaggatggcc
     3001 ttcttatacg aacctaaact aataatcggt acagtcccaa gacccctgtt gccgctgaaa
     3061 taatcagcgc aaaaaaagct accacaaaaa gatctcggaa aagtatgttt tgattatgtc
     3121 tgcccttcat gccttctgct tcatcttcca tagccacggc tgacataatc agagcgccta
     3181 tcgtagaaat aggactgaag ccggttatcg ttccgccaat aacaaccatg gcaacgagct
     3241 ctgtaggatc taccccggga agagatgcag caatcttacc tgtcgtcgga atcagtgtcg
     3301 ggaatacgac gccaagaccg gagctaaaca gggacataac acctgatatg agaaccataa
     3361 tagaaggtgc cgtttgaacg gtcatcatgg aggttaaaat agaagccaag aaatcgacac
     3421 ctccagaaat caacatgact tgcatcaaca tgccgacccc caggaccatc aagataatat
     3481 tccaagggat cgctttgatt acagcttttt catttccata gccaagagct agtaaaatcg
     3541 tcccaatcaa gaaagccgta agacctacat tcatcttaaa taccaaaaca cagatgagca
     3601 gtgccgctaa gcccattaac gtaacaattt gatttttatt aaacgttaat tgactttcca
     3661 tttcaacgtc agctataggt tcgctgactt tccatccttt aaagtaaata aagcagctga
     3721 taaagataaa aacttcgcag atcagcataa aatacatgat tggcacggtc gcatacgtag
     3781 tcgccgccaa cccttggtct tccatcaact gtagtacgac agccgcttcc ggcgtaatgg
     3841 gagacatgcg tccgccctga acgcccatct gtcccactaa gcagagtaat atcggattca
     3901 aacctgatga gcgggcaata ggaagggcca acacgggaat gatggccagt gacgggatgg
     3961 ctccgggacc tgccgcacaa atcaaaactc cgctgataaa catcatgata taccccatca
     4021 tcttccatga tccgacgagg cgaacgagtt tcgatgctaa aatggtaagt gaatcatttt
     4081 gagataaaat cgaaaataaa taggttacgc ctacgatggt cagaaaaagg gatgtagaaa
     4141 aacctttcat aatcaggctt ggcttaatct gataaaaagt ccccagcaaa acagccatac
     4201 cgatacagag aattccgatg ttcgtcttgc gcactacccc gattacaatt gccaaaagca
     4261 atgcaataag cgatacaaca ctagcactca ttgggagcac ctcccagcgg atagagttcg
     4321 gcccaagaca gccagttccc ctgttcatgc aagtcatagc gtctcacaaa ggaaagcgtt
     4381 ccatccgaca taatctgaaa tactgaaagg caggccggtt caaaacgcca accgtctcca
     4441 tcaggaaccg tcatcgattt ttgattgact gctataaata cagtcnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnngt gattctgcac catggcagct gctgcggccc attaatttag gttctcccat
     4621 ctgttcatcc aatttaaaaa tggcaatatt attttctcca gccaccagcc ggtcttgccc
     4681 ctggtaagtc tccgttttat cggctcggtt tgatacataa agatattttc cattgggatg
     4741 aagaacgcct acaccgcctc gctgccgtac cgccgtacgg ttcgaaagag actgcagggt
     4801 ttccgtccta aagatcggat caccgtcaac aagtccattt ttcagcgcat aagtaaaaat
     4861 catagagcca cgttcgatgg ctaaataccc ccacttatgg tcagcactga aaatcagatg
     4921 ccgcggaccg aattctacag gaaattcttt ccgcccttta aagacagctc gcccgttttc
     4981 tgaggaaaag gaaaatacac ttacttcgcc tcgttcctcc ggcttgcctt cctgtggcgt
     5041 attccctctt gatatcgtaa tgatgtgttt ctgatccgga gaaaaaacag cttgatgcgt
     5101 aaagataccg gcctttaaag gagcatcctg ttcaacctca ccgataacgc ttccgtcgtc
     5161 cgccagccga tataagcgaa tctgccctga cttattgaag gaaacagccg cgtaacggct
     5221 agtctgatca agactgatgt gaataggtcg ttcaggaagc ggataatact cccctttcag
     5281 agaaagactg ccatcttccg ccccgatttc cattacctgc aggccatgga tgg
//
LOCUS       DAWDXZ010000061         5324 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4277_length_5324_cov_11.054470, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000061 DAWDXZ010000000
VERSION     DAWDXZ010000061.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4277_length_5324_cov_11.054470"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1077..1086
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1298 a         1250 c         1515 g         1251 t
ORIGIN      
        1 attacgatga taccagaaca ttattgggcg ccgttgagcg ctcttttaag ccgacgacta
       61 cactgatcga tgtatttttc ccggaagtac aaacgttctc cacagaatac gtagatatgg
      121 aattccggaa aggcggccga ttgatggcgc cgtttgtcgt tccgggcggt aaaggcgtga
      181 acatggcgcg taccggctcc attatccgat cctataaggc tcccttgatg cggcctaagc
      241 gcaatatcga gcctgccgac atcattatgc gtggatttgg tgaaacggtg tactccacta
      301 aaacgccggc agaacgtgcc gctgaaatcc gagcccgtga cctcagcgaa ttgattgata
      361 tgtgtacacg tcgtcaggaa tggatggctg cacagctact tattaacggt gagtacgatg
      421 tgcagggctt cgccgatgac ggtagtattg ctcatgtgga cacgatttct ttccctgaat
      481 tctccaataa gacaacgtta tccggctctg atacttggga taactcgact gcggatattt
      541 taggggccct ggaagcagaa tcccagaaga ttcgccgatc tgccggtatg atcccgacga
      601 tggctatctg ctctagcaca gtagctaagt atatcatcaa caataccaag ctgcgggacc
      661 ttatgctgat tcccagccgc gataacatgg cactgatgag cattcagccg cagtttgtcc
      721 gcccggactt gctccgtgtt gggtatattt ccgcgttgtc gcttgaactc tacacgtatg
      781 acggtgggta tctggatacg gacgataact tcatcccgta tatcccggat gattacatca
      841 ttatgggcgt ccccggccgc ggtaaacgtc tttttggggc tattacgcag ctcgaggcag
      901 atggccagta tcacacctat gccggcaatg agtacgtacc taaggttacc gcagacgtgg
      961 aaaatgatgt aagctcgttg gcgttgtcca gccgctgcgt catctgcccg gaattccttg
     1021 atgactgggc tgtaatcaag gtcaaatagg aggctgccat gaaaattttg gttaaannnn
     1081 nnnnnnggca gtgttgctga ccttccggat gacgtcgcta aggtattagt cgaagcgtcg
     1141 cctaaggaat tttcggcggt ggatgcggct gatattgccg cgccgtcggc gcccgttgaa
     1201 gatgtgcctg tcgacgactc ggaggtagta gagttgccgc ctgtggattt tgctaaaaat
     1261 cggaaaaaat gagccgccga gacgacagaa tagctgatac gctggctaag ttgtaccagg
     1321 acgacgtaca agagcaggac agcgctgaaa agcaggttac ttttaaggat catgtagcta
     1381 tggacgctga tatattcatc aatctcgatg aatttgccga cgaacataac ctcaatggtg
     1441 atgtcgttaa ggccatcgta gagagcccga cgagtcagga aaaatggctg actagcaggc
     1501 agtataatct gtacgatggc cttgagggca tgttgtacat tgtgcattgt cgtaagcagg
     1561 acctgtccat ggtgccggta tatggccagc gatttgacct tgacggcact atctgcatcg
     1621 tcgacagcgt cattgatgac atgggtatcc ttacgattga gctgcatgcg gaggtgacgt
     1681 aattgattag tatcgactta accggtaccg gacaagtgga gcgggtgctt cagggctttg
     1741 ccggcggcgg gaaagtactg agtgctatca agcaggcatc ccgtcgcgca gctgttacgg
     1801 cgcgtaaggc cggggctcag gagatccgga gcatgtacac aattaaggca ggcgacctta
     1861 agagtgcggc agttattaag ccggacgcct tagggacgac catccacatc agaggcccgg
     1921 aagagccggt aactaagtac aaggcaacac gtcgccggaa ggggattttc gtctcgatta
     1981 aaaagggcag tggctctatc gttccgcggt cctttgacat gccggggcgc ggttttgcgg
     2041 ctcgtgaagg acggcccaga tatccggtaa caggcctgtt cgggccggct gtacctcagt
     2101 tgtacggcaa tcctgccgtc gtagcgagga tgaccgacga aggtatggcg atgtatgaaa
     2161 aacggcttat gcatgagctg gaacgattgg caggtggtta gcaatggtgc cattggacgt
     2221 aattactgat ttgcaggaat ttctgaaaaa caaaatgcaa gcatatagtg gtcttaccgt
     2281 agacggtgag gccattaatt gtttcagggg gtttttaccg cgggcgatgt cgccggaggt
     2341 caaagagcgg ttatgcccgg caatcgttgt tgggtattca ggcgtcatgg atcgggctga
     2401 tgaatccatt gtatcggtcg tcatatcggt cgtcactcgt gaccaggaca tgctccacgg
     2461 cgccgatgag ctgttccatg tgctggagta catccggcat tgcctgctga gtaataaccc
     2521 ggtatgcgat aagtacgacg tcaaaaacgg tacgatggaa acgtcggtac cagatgagca
     2581 accgtatccg caatggtggg gccggattga ttttgaggtc catatcccac agccagataa
     2641 tattgacgcg tttttgttgg gaggtccaga aaatggatga caaagaaaaa gtagcagctg
     2701 ttgaaaacgt ggacacaaaa gaaaatgtgc ccacaaagaa ggctgcacga tacactgggc
     2761 cggttatcta tattggccct ggattccgtg actcacggct gaatcattgc atgatttttg
     2821 ccggcggcat cccgtcgccg gaatcggacg acgaaatttt aaaacatctg ttcgtgccgc
     2881 cgtctgagct taatcaggcc ttggcagatg taagaactaa ggggactgcg ctgaatacgt
     2941 tctatcgggc ggcagtcgac cgacaaggag gtaagtagat atggcatttt ttcacggcat
     3001 caagacgtcg gaaatcgata cggcggttgt tgcaacagcg cagacgactg cggggcttcc
     3061 ggtagtcttc gggacggcgc cggtgcattt ggccagtgcc ccgaaaatta atgagcctgt
     3121 cctctgctac agctggacag aagcggtaca gcaattgggc tatcatgccg actgggataa
     3181 gtacacgtta tgcgaggcga tgtacgccga atttaagctt ttcgctgtcg caccggttgt
     3241 atttatcaac gttctggacc cgtcgaagca caaaaagacg gtgcccagta ccgcagtcac
     3301 ggtagctaat aagacggctg ttatcgaggc tgacgttatc ctgagtacgc ttaaggtatc
     3361 cagtacatcg tctgggaccg cggctacggc aggaacggac tacacggcag cctataacga
     3421 tgacggcaac gtacttatca cgttgcttaa ggacggcgca ctggctgata gcacgactat
     3481 ctatgttgct tatgatgcgg ttgacccgtc tatggtcaag gacgctgata tcatcggcgg
     3541 tgttggcagc aacgacgcgg caaccggcat ggagctgctt gatactatct atcctaagac
     3601 cagtcttgtc ccgggcctgt tggccgcgcc tggatggagt catcatccgg aggtcgccgc
     3661 agtcatggca gcgaaaagca agaaatttaa tagcctgttt agctgtaccg tcttagtgga
     3721 catcgacacg acacaggtta agtcctacag cggttgcaac atgtggaaaa ctggcaacgg
     3781 ctatacacag aataatgagt acgtcggctg gcctatgggc cgcatcggcg accactgtta
     3841 ttacatgtct acgcttgcga tgggccgcat cggtcagacg gacgccgcca atgacgatgt
     3901 gccgtatgag tctccgtcta ataagactct gccgattacc ggcttatgcc tcaaggatgg
     3961 taccgaggtc acattggacc ttacacgggc taacttgctt aacagccagg gcattgtaac
     4021 ggcgattacg tcgccggcag gctgggtgct gtgggggaac tataccggcg cgcatcctgg
     4081 cacgacggac cccaaagatg tatttctctg cgtccgccgt atgttcgact gggatgatac
     4141 cgtattcgtg ctgacctact ggagtagact cgataagccc ggaaaaccgc gtaatatcaa
     4201 gactattttg gactcggaac gtatccgttt gaacggcctt gtctcgcgcg aatatatcct
     4261 ggacggtagc attgaatttc tggaagaaga aaacccgacg acggaccttg cggccggcat
     4321 tttccgattc cataaaaagc ggacgccgcc cgtgccgatg caggaaatcg atacgatttc
     4381 cgagtatgat acatctgcat ttgcggcgtt attccaataa gggggtgagc atatatggcg
     4441 attaataaaa tgcctgaagt gcttaatgat tttcggctgt atgacgagtc gggaggcacg
     4501 ttatacggcg tggctaagct ggagttgcct gattttaaat cgatcacgca gaccgttaaa
     4561 ggtgtaggcg tcggcggtga aatcgaggca ccggtacttg ggcaatttga atcgatggaa
     4621 accaagattt cccacaacat caatagcgag tggaatctta aattagtcgg cggtcaggcc
     4681 gttgccttgg aagcccgcgg tgctaatcag tactgggaca gcgcggcgaa caagtacatt
     4741 attgacacca tccgcgtcgt tatccgcggt cgctctaagg caatgtcggg cggctcttgg
     4801 gagcctgcca gcactgtcga ctccgacaac accatcgaga cgacatacat taaatacgag
     4861 gtcaatggca agactatcct cgaggtcgat aagtatgcca acaaatttat tgctggcggt
     4921 gaggacttga cgcaacagat tcgcaaagcg ttaggcatct aggaggcgca tgatgaaaaa
     4981 agaagttgaa gtagtagaaa ctaccgtcgt agagacgcct gaaaaggcgt tggcggtgcc
     5041 gtccgattgg gtcgtcgagt tgtctaaggc cctgccggat ggcaagaaaa cactggtact
     5101 ggattttgac aaaatcacgg ggtataccct tcttagctgc gaaaaggccg cgaagaaaga
     5161 ggacgccggc atggtcgtac cgtctctgtc tcaggtgtat caggcccatg tggcggctat
     5221 cgcagctgat gtcaagtacg acgatattct ggcactttcg gcacgggatt ttaccgcggt
     5281 catgattaag actcagggtt ttttattagg ttcgggctcg cagg
//
LOCUS       DAWDXZ010000062         5286 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4306_length_5286_cov_9.089658, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000062 DAWDXZ010000000
VERSION     DAWDXZ010000062.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4306_length_5286_cov_9.089658"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4648..4657
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1256 a         1440 c         1400 g         1180 t
ORIGIN      
        1 gtcaaagaaa tcgtacaatt cttcgaacaa agcccgatta gagaccgccc ttgcaaaaaa
       61 aacgacagcc cgcttgttct cttgaaaatt ccgaggtccg tatataagtc gcgacaagtg
      121 ccaaaaatag gaaatactac gcatgaaatt cctcctatca agtacaatcc aatagaccta
      181 ttatacgggc aggccggcgg gctgtcaatt attcatgttt taaaagagct tataaccgtc
      241 ttaagatgtc cggcgatacg ttggacaaat catgaacgcc ggtaatcatc atggcatcca
      301 tgatttcccg tttcaaagca tctaagtaga gcttgacggc ttcagcctgg ccgccgatag
      361 cggcgatggc gacaggacga ccgatgagga cggcgtcggc accgagggcc aacatcttaa
      421 ggacgtcttc cccatggcgg acaccgccgt cgacgaagat cgtaagacgg ccttttacag
      481 ccttggcgat ttccggcagg acgtcggccg ttccgaccat accgtcgagg atgcggccgc
      541 cgtggttgct gacgacgatg cctttggcac cggcatcggc acaggcaacg gcttcttcag
      601 gagacatgat gccttttacg ataaagggca gggacgtcga tttgaccagc tcggcaatgt
      661 cactggcgct cttagggccg acgggctggc caaagatatg catattgatc agcgtcgacg
      721 catcgatatc gctggcaacg gcaaaggcat gggccttttc agcgactttt atcttttcaa
      781 tgatctggtc gttgctgcgc ggtttgatcg tcggaatgac cgtttcttcc cgttttttcg
      841 aagctacgat ccccgattca aacatgtatt ccatgccgcc gtcaccggta aagcacaggg
      901 tgccggcatc gaaggcgcct ttggaaacgg actcgttata atccatttca accgtagccg
      961 ggtcaccggg gaccttggaa ttgaggatga cgccgccgac gggagcgacg aggaccggca
     1021 tgctgatttc tttaccgaaa atcgtaaccg ccggattggg gtcaagttca ctgtccatac
     1081 tgcgcatgac caggccatat tcggccagtg cttctacgtt gcgcaggaac gattttgccg
     1141 tccgcagacc gccaaagccg ggaatcatcc ccttacaggc cttgccgtcg cattcgggac
     1201 agacgaagca gcccttcatc tttttgcgag cgagttcgcg agcttcttgt aaatccatag
     1261 aaaaaccctc cttcagttta taaaatatat aaaaaattta aatagtaagc aaaaataacc
     1321 gttttcagga ctagtcttcc cttgtttatc ccaatgataa acaaatttca tacgaggctg
     1381 ctgccagcga atgtaacgca gctgccgcgg agacgtaagg tgcttaacgg gtaaagattt
     1441 tattaaaagc ccgtgtccta taattctgct ccagcatcct cacatgagac aagaaggccg
     1501 gatcgtcaaa atacttggct ccatagggac aggattggac gcagcgctga cacttgatgc
     1561 agatgcccgt tacccggtcc ggccggtcct tatcgatgga acccatagga cactggcggg
     1621 cacagaggcc gcaggacgtg catttttcca tatcagtctt agggacggcc tttaagaatt
     1681 tcgccggctg cccgtcttcc cctttaggaa tatagtaagg ggcttcggca tcgccgtcta
     1741 cgaccaggga atcggtcggc cgattttctc cttgaaggaa gtgaacgacg ccatgggcca
     1801 agctcctgac gtcagccagg tcatcctcat cgggacggcc tgaggccagt atcttagaaa
     1861 aggcatgctg ggcgacaaag gcaccggcac cgacgaggtt gaaatcgttt tcctttaaga
     1921 tggcacagag ttcggccagg gaattgtcaa aggagcggtt gccgtaggtc accatggcga
     1981 cagccggcgt ttttttaccg aataattttt ctctgtacgt cggcataatc ttattgggaa
     2041 gctttccggc gtaggtcggc ccgccgacaa cgacgagatc ctccgcggaa aagcgatgtg
     2101 tctcttttcg ctcagccgga aggtccaagg atatcgtctc aaggggcacc tgaagggcct
     2161 cggccacctg gccggcaagg gcgcagacga tgtccttcgt gtgaccggtc gggctgaaat
     2221 aaacagcata gactttctga atcttcaaat aaatccctcc caacacgagg aacgataagt
     2281 tgtatacctc cattatatag aaaacaggcg ctaaagtcac tgactgcgcc tgttttcttt
     2341 ttattttttg tggtacaatc aaatccgtaa ttgacgacga aagaacgagg tgtctcatga
     2401 aaaagaaagt atatgccgtc cggaacggcc gcaagacggg attattcatg tcttgggatg
     2461 actgcaaaaa acaagtcgat gccttcccgg gggctcgcta taaaagtttt accgatgccg
     2521 acgaagcctg gaaatggttg aacggtgaag acgtactatc ggttcgaaaa ccggtgcgat
     2581 caaaggctcc gaccttaaga ccgacgacag cgtccctgcc ctccgatgag gaccaggatt
     2641 ttgtcatcta caccgatggg tcctgcctgc gcaaccccga cggtcccggc ggctgggccg
     2701 tcgtcgcccg caacgtggcc acaggcgaag tgaccgaact ctcggacggc catccgtcga
     2761 cgacgaataa ccgcatggag cttcaggcgg ccatcatggc cctgcgcttt gcaccggaag
     2821 gcacaaaagt cgccctctat acggacagtc aatatctgaa aaacggcatc actaagtgga
     2881 tggtcaattg gaagcgccgc ggttggaaga aagccgacgg ccagcccgtc ttaaaccaag
     2941 gacaatggat ggaactcgat aagctctacg gctctcataa agtcaccttc cactgggtca
     3001 aaggccacgt cggcgtcgcc ttaaatgaac gctgcgacca actggccaag gccgaagcga
     3061 tgaaatatcg ctgagacgtc agcttacggc acggatgagg ttaaagacct gctgagccgt
     3121 gcgggtcatg ttgcccatag ctgtgtcgcg gtccatggct tcctttaagg gcatggcctt
     3181 atcgacgatg gaaaagaagg cgtcgatgcc ctgccggtta cactcgccgg catcttccgt
     3241 cgtgcagccg gccagggcga tgaccttagc cccgtactgt ttggcaagct tggccacacc
     3301 gattggggcc ttgcccatgg ccgtctgggc atcgaggcgg ccttcgccag tgatgacata
     3361 atcggcatcg gcgacgacat cggctaattt aatagaatcg agtacgattt ggatgcctga
     3421 acgaaggctg cctttcagat acgccaagaa ggcaaagccc agtccgccgg ccgctccggc
     3481 accggaggcc tgactgtagt cttttcccaa ataggcttcg gtaacggcag caaaggaacg
     3541 gctggcctca tctaaagctt tgacatcgga cgctgacgcc cccttctgag ggccaaagac
     3601 agcggccgct cctaactccc catataaggg attggtcacg tcacaggcga cgtcgaaggt
     3661 gcattctttt aaggccggca gaacatgact gtcgtcgacg gaggcgatgt cccctacggc
     3721 aaggcccccg gtcccgacga tttgtcctcc tttatcgcga aagacatagc ctaaagcctg
     3781 gagcatgccg acgccgcagt cactggtcgc actgccgcca aggccgatga tgaagtggcg
     3841 gatgcctcct tccatggctt tggccatgac ctggccgaca ccataggtcg tcgtatagag
     3901 aggattgcgc agctcttccg gtaccaaacc gagaccggca gcccctgcca tttcaatgac
     3961 cgccgttctc gtctcgggca aatatccgta ggaacaagaa acatcatttc ccaaaggacc
     4021 ctgaacgacg gcgctgcgaa gctccccgtt catgccttcg acaagggctt cgacggtccc
     4081 ttctccgcca tccgccaagg gtttgacgac gatctgtacg ggctggcttc cataggcttt
     4141 acgaacgccg gcttcgatgg ctctcccggc ttcgaccgac gtcagactat tttttaatga
     4201 atcgatggca atgacaactt tcatattccc cactccttta tacactatga gttatctaca
     4261 tatttattgt gcctctagtg taacagagga gttttttcat ttctatgtta cccataccaa
     4321 gaaacgctgc catttttcgg ttttagaacc atggcagcgt tgcgatatac agggtgacgg
     4381 catcgccgaa agaccgcggg tcgagacccg tcgtctcggc gatttttttc agccggtact
     4441 ggagggtatt cttatggata gagaggatgt ctgctgtctg ctgaatggaa aggttttgcc
     4501 ggtaatagac ctgcagcaaa tgaatttcac tgtccgtcag gccctggcag acatcctgcc
     4561 gatagcggcg ccgaaggggt gcgctcaggc tctgcagtaa aaaggccagt ttcatgtcgt
     4621 cggcatggac actgaagacc tctcggtnnn nnnnnnncct ctcggtcggc cgcggcctga
     4681 agggctaatt tggcaaagcg atacgattca tagacgtggt gaaaaggctc ttccgtcccg
     4741 atcccgacgc ggacagacgg ataaaactgc cccttccacc gggacagccg cttatcccaa
     4801 agaggataga tatcttgcgg tatgatgagc acgaagctgt cgggatagat ataggtaaag
     4861 agggaaatac ggagttcccc gcaagctgtc gtcatggcct tgcgaaaatc gtcggcacgg
     4921 ccggccccat cctgccaacg aaagacggct gccacatagg aatgttccgt cagttcgggc
     4981 cgttcgtcag ccatctgctg catcgtatcc cggtcatgat aaataagggc catgacctgg
     5041 cggctgcggt actcctgttc actgaaggct tcttcattga ggccgtattc tttaaggaat
     5101 acttcacaga tcttggtcag caaaaaaccg tactggctga cgacttccgg ttcacccgta
     5161 ataccgatga cgccgaggac ttgccggtcc atgataatcg ggtagttgac gcccttactg
     5221 acaccatctt ccggtcggtt ctcatgaacg gtttgaacct ggcgcagcct ggctgccgct
     5281 gccccc
//
LOCUS       DAWDXZ010000063         5237 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4335_length_5237_cov_10.175994, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000063 DAWDXZ010000000
VERSION     DAWDXZ010000063.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5237)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4335_length_5237_cov_10.175994"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1167..1176
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3532..3541
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1290 a         1291 c         1367 g         1269 t
ORIGIN      
        1 agaaacaaaa tctatttatg ttttttcgag tgtctacttg acgggtagca gttcacgaac
       61 ggtaggagct ttttcgctgt atcagcggat aaacttgatg aaattgtctt ttctcggaga
      121 cgcagccggc tccggcccgt cgccgtatcc gacgatacag tggccgacgc cgactaagtg
      181 ttccggaagg ccccattctc gaagtaaaga ctttccttcg gggagttcaa attcttcacg
      241 ggctctgttt atccaacatg aaccgaggtg cagggctgcg gcggcattca taagattacc
      301 taaaataaga cagccgtcag accaggcgtt gggggacttt tccatgtcgg ccagaacgac
      361 cagggccgtc ggggcaccgt acagaggatc cgtgtcggtt cctttgattt tggcattcat
      421 ggccgtcaag cggcctaagg tgtccggatt ttgaacgacg acgatggtac agggctggat
      481 tcccttggca ctggcggcat tcattccggc ttggaggatg aggtggagat cttcgtcgca
      541 gatttgggct tttttgtatt ttcggacact cctcctgttc gtaagggtgc ggatggtttc
      601 gttcataaaa atcactcctt gaaaagttgc ggcaattcga ttcgagagct cgccgctatg
      661 attgtttgtc agtcctatta taggtcgtcg acgttggcag gaaaagggga gggatgagac
      721 aaaaaaggat tcttttttcc tgtcaggcag tctccggaga cggggcctta atctattgct
      781 tgatactggc gatttgatgg aaaaatatgc tataataaaa cgattagata gtgatgaaga
      841 tagtatctag gagcgattgc attgaaaaca caagcaccga agctttcaca ggagtatatt
      901 caaccgctgg ccgatgtctt caaggttttg ggagatccga cacggcttcg cattttacgc
      961 cttctcatgg atggagaagt ttgcgttcgt tgtatttctg aagaacttgg catgggtcaa
     1021 tcggctgtat ctcatcaact gcgtattttg cgggatgccc gcttggttca gttccggcgg
     1081 gaaggtaaga acgtgcagta ttccttggcc gatgcccacg tatttacgct gctcaacgtc
     1141 gggttggagc acgtcgccga ataaggnnnn nnnnnnatgt atctttgttt aatgcgccac
     1201 ggaaaggcac agcctttttc tttaaaaaag ccggataaac agcgggaatt gacggaaaaa
     1261 ggctgctgtc aggccgcttc tatgtcctct tgggccgcat cgtggtggcc ggagggaaaa
     1321 acggcacttt gggtcagtcc ctatgtacgg acttgccaga cggcatctta tttggaacaa
     1381 gagctgccaa tcgggcagtt ccatacccat gaagccctgg cttcgggaga actcgttgcc
     1441 gtatatgatc aaattttgag tcaggaaaaa tcggacgtcg tctgcatcgt cggccattcc
     1501 cccttcctcg accgctgggt tcaggcctgg acggggacag ccgtcgactt caagaccggc
     1561 agcatggccc ttttggactt tgacgtccat ggcgggcgaa tcggctcggc ctctttgctt
     1621 ttctacgttc atccgaaagc cttgaaattt ttggaggcgt ctcgtaaaaa ataacgatgg
     1681 ttggaggttt tatctatgat gaaacatgta ttgtccattg ccggtaccga tccgtccggc
     1741 ggagccgggg cggcagccga ctgcaagacc ttttgtgctc acggctgctt tgccttgaat
     1801 gtcattacgg ctgttgtttc acagaataca cagggcgttc gcggatatat ggacgtcaca
     1861 ccggaactga tcgcatctca aattgatgcc gtatttgaag atatcgacgt cgatgccgtt
     1921 aaaatcggca tggtcagcgt gcccgaaacg attcgggtca ttgctgaaaa attgaagcag
     1981 taccgtccgg ccttcgtcgt catcgacccg gtcatggttg ctaccagcgg ccatcgtctg
     2041 ctggctccgg aagccgaaca gaccttgaag gacgtattgc tgcccttagc cgatgtgttg
     2101 acgccgaacc ttccggaagc ggaagtattg accggtaaaa agattgaaag ttttgaagat
     2161 atggaagggg cggcgaaagc cattgccgcc atgggagcta aggccgtgtt ggtaaaagga
     2221 ggtcaccgca ttgccgatgc gacggatgtc ctgttcgacg gcgaaacatt ccattactat
     2281 aagggaaaac gcattgaaag cacgagcact catggaacgg gatgttctct gtcgagtgcc
     2341 attgcttcta atctagccaa cggcatgagt ctgtccgatg ccgttcaggc tgctaaaacg
     2401 tatgtctttg aaggaatcgc ccatgccgaa ccgattggca aaggccatgg cccgatccat
     2461 catttctatg aattatataa gaaagcaggt gtcaattgat atgaacatta catactcttt
     2521 gaaaaaggcc tgctgcctgg tcatgacggc agcttctatt ggcggcgtcg ctttggcagc
     2581 aacgcctcag gatacgtatg aagaagcggc ccgcaatatg gcggctcatc cccagggggc
     2641 ttataccttg accttaggta tcaagatgcc cttcgtcggt gacggcgccg tcgtcaatca
     2701 gatcaatatc caggaacagc ctttcgtcgt cgaatcgcaa gtaaagaccg aaggcttcgc
     2761 ttcccaggcg atggataaaa ttcaccagaa caaggcttat ggcgtccaga acgggaaaaa
     2821 actcgacgtc tactatatgg accaggaaaa agacggccaa tggatgaaga aaagttatga
     2881 cttaagggat tctaaaccca ttgccagcta tttgacgaac gatcacaacg tcctttctgg
     2941 cgtaaaatcg gttacccgtg ccggcggaaa cgactatcat gtcgtctttg atgcatcccg
     3001 tctctatacg gcctcggatg aagccaactg gaagaaggaa ggcatgaccg atgaacaggt
     3061 ccgcgtgttg gccaaggtcc ttcagggact gcagaaagtc ggcgacgtac agactgtcgt
     3121 taccatcgat ccttcgacga agcgcatttc ccgcatcagc ctgcccttga cggaccagct
     3181 ccgaagcttg gccttgacct tggttgacga atttggccgc aatgatgctg aaaaagccgt
     3241 atcccagtct ttcatcaaaa tgagcgaagt caatttgacc attgattata cggccttgcc
     3301 ccagggaacc cggctgactg tcccggaaaa tatcatcaag acagctaaaa ccggaaagta
     3361 attgtaaaat ttttgtgtaa gaatgaagga ttattcaaaa aaaataagct agattgttga
     3421 cagcgtcccg gaaataacgg aaaatatgat agaaggaatt atttccacta ctgttcgttt
     3481 tatcttcata taggatagac ctgcagctcc gatagggggc tccggttgga annnnnnnnn
     3541 naactatatt tatttttagg aggtatattt tggctaaaaa agaaaaactc atggtaattc
     3601 ccttaggtgg cttaggtgaa atcggcaaga atatgacggt tatccagtac ggaaacgata
     3661 ttatcgtcat cgatgccggt ctggccttcc ctgatgacga tatgttcggc atcgacctcg
     3721 tcatcccgga catgagttac ttgattgaaa accgggacaa agtacgggct gtcgtcatta
     3781 ctcatggtca cgaagaccat atcggcggct tgtcctactt actcaatgaa gtaaacgtgc
     3841 ccgtgtacgc aacgaaactg gtatgcggcc tcattgaagg gaaactcaaa gaaaaccaca
     3901 ttacgaatta caccttgaac gaagttcatc acggtgacga agtacagata ggctgtatga
     3961 aagtcggctt tatccacacc aatcactcta ttcccgatgc cagtgccctc tatttccgca
     4021 ctcctgtcgg aacgatcgtc catacagggg actttaaagt cgatatgaca ccggtcgacg
     4081 ggaaacagat ggacatgcac aaatttgcag aattaggccg ccgcggcgta ctgctcctga
     4141 tgtcggacag taccaatgcc gaacggccgg ggtacactga atcggaaacg acggtcggcc
     4201 atgctttccg caaggccttc cgtgcagcta agggccgtat tatcttggcg acattcgcct
     4261 cgaatatttc ccgtatccaa caggctatca atacggccat ccaattcaaa cgtaaggtaa
     4321 ccgttctcgg ccgcagcatg gtcaacaacg ttcaggttgc cattgaactg ggctacctcg
     4381 atgtgcccga aggcgtcctc atcgaaccgg atgagctcaa ccgttatccg gctgaccagg
     4441 tcctcatcct gacgacgggc agccaaggcg aaccgatggc cgggttgtcc cgtatggcat
     4501 ctaataacca ccgcagcgtc agcatcatgc ccggcgatac ggtcatcatt tcggcaacgc
     4561 cgattcccgg caatgaaacc ggtgtcggcc gtaccatcga caacctcatg cgcctcggtg
     4621 ccaatgtcat cgccggtcgg gataagaaaa ttcatgtatc cggccatgcc agccaggaag
     4681 aactgaagct catgctgggg ctcattaagc cgaactactt cgttccgatt cacggtgaat
     4741 accgcatgct tcggacccat ggcgacttag ctgtcatgat gggcgtcgac aaagatcacg
     4801 tcctcattgg cgacaatgga cagatttttg aattttccaa tcgctccggt cataagacgg
     4861 gccatgtcaa tgccggccgc gtcttcgtcg acggcctcgg tgtcggcgac gtcggcaaca
     4921 tcgtcatccg cgaccgccag cagctggcta tggaaggcgt cgtcattgtc gtcatgacct
     4981 tggcaaaggg cacgagccat cccttggccg gtcccgatat cgtatcccgc ggcttcgtct
     5041 atgtccgcga ttccgaagaa ctgatccgcg aagcccacga ccgggttgcc gctgttctcg
     5101 aacgctgtga agccggaaat attcgcgaat gggctgtcat caagtctcaa gtacgcgata
     5161 ccctcagtcg gtatctctat gaaaagacgc ggcgccgccc gatgatttta ccgattatta
     5221 tggaagtata aaacgtg
//
LOCUS       DAWDXZ010000064         5233 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4342_length_5233_cov_9.773078, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000064 DAWDXZ010000000
VERSION     DAWDXZ010000064.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5233)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4342_length_5233_cov_9.773078"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1325..1334
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1380 a         1348 c         1301 g         1194 t
ORIGIN      
        1 agatttcagg ttttatctta aggctttact gtaaattaac tatacactaa ggagagtgga
       61 cacatcatgg attttaactt aacagaagta caacaggatt tcatgaacct cgctagagat
      121 ttcggtgaaa agagattagc tcctacgatc atggaacgcg accatcaggg catttatgat
      181 gacgcactga tcgctgaact gctcagcttg ggcatcacag gcgcttactt tgaagaaaag
      241 tacggcggtg ccggcgatga cggcggcgac gttttgagct acatcttggc tgttgaagaa
      301 ttagctaaat acgatgctgg cgtttccatc accttgtctg caacggtttc cctctgtgct
      361 aacccgattt ggcagttcgg cacggaagaa cagaaagaaa aattcttggt tcccttggtt
      421 gaaggcacga aactcggcgc tttcggcttg accgaaccga atgccggcac ggacgcttcc
      481 ggccagcaga ctgtcgctgt taaaaacgac gacggcacgt acaccctgaa cggctccaag
      541 atcttcatca ccaatggcgg ccatgctgat atctacattg tcttcgctat gaccgacaag
      601 agcaaaggca accacggcat cacggccttc atcctcgaag acggcatgcc cggcttcact
      661 tatggtaaaa aagaagacaa gatgggcatc catacgtccg tcacgatgga actcgtattc
      721 caggacgtca aagttccggc tgaaaacatg ctcggcgaag aaggcaaagg cttcaagatc
      781 gctatgatga ccctcgacgg cggccgtatc ggcgtcggcg ctcaggctct cggcatcgca
      841 gaagctgctt tggcagatgc tgttgaatac tccaaacagc gtgtacagtt cggcaaaccg
      901 ctctgcaaat tccagtccgt ttccttcaaa ttggctgaca tgaagatgca gatcgaagct
      961 gctcgcaacc tcgtttacaa agctgcttgc ttgaaacagg aaggcaaacc gttcacggtt
     1021 gaagctgcta tcgcaaaacg cgttgcttcc gacgttgcta tgcgtgtttc cactgaagct
     1081 gtccagatct tcggcggcta tggctacagc gaagaatatc cggctgctcg tcatatgcgc
     1141 gatgctaaga tcactcagat ctacgaaggc acgaacgaag ttcagctcat ggttacagct
     1201 ggtgctctgt taagataatt gaagtttatg atgcaggctt ggcatcagcc aagcctgttg
     1261 cataaaaaaa gactttagga ggcaaacgta aatggaaata ttggtatgtg tcaaacaggt
     1321 tccgnnnnnn nnnnacgtca aagtcacgtt actctccatg ggtcctgaac aggctaaaga
     1381 tgtccttcgt gaaggtctcg ctatgggtgc tgacgatgct tatctcgtat ctgaccgtaa
     1441 actcggcggc tccgatacgt tagccacggg ctatgctttg gcacaggctg ttaaaaaagt
     1501 tgctgaactc aaaggcatcg aaaaatttga tttggttctt tgcggcaaac aggctatcga
     1561 cggcgatacc gctcaggttg gcccgcagat cgcttgcgaa ctcggtattc cgcagattac
     1621 gtatgctcgc gacatcaaag tcgaaggcga caaagtcact gtccagcagg aaaatgaaga
     1681 aggctacatc gtaactgaag ctaatttccc tgtcctcatc accgctgtaa aagacttgaa
     1741 tgaaccgcgt ttcccgacca ttcgcggcac gatgaaagca aaacgccgtg aaatcccgac
     1801 cttagacgct gctgctatcg gcgttgacga aacgcttatc ggtttgagtg gttctccgac
     1861 aaaagttcgt aaaactttca ctccgcctca gagatccggc ggtctcgttc tcaaagttga
     1921 agacgacaac gaacaggcaa tcgtcgacca gctcatggaa aaactgactg cccagaaaat
     1981 catttaattc aaggaggaac agtgaaaatg gatttagcag aatgcaaagg catttatgta
     2041 attgctgaac agtttgaagg caaattacgt gatgtatctt ttgaattgtt gggtcaggcc
     2101 cgcatcttgg ctgacaccat tggcgacgaa gtcggcgcta ttcttattgg taaagatgta
     2161 aaaccgttgg cacaggaatt gatcgctcac ggtgctcata aagttttcgt ctatgacgat
     2221 ccgaaactcg aacattacaa tacgacagca tatgcaaaag ttctttgcga cttcttcgaa
     2281 gaagaaaaag ctaacgtatt cctcgtcggt gctaccaaca tcggccgtga cctcggccct
     2341 cgcgttgcca acaccttgaa aacgggcctt acggctgact gcacgcagct cagcgttgac
     2401 gacgacggca agaccatcgt ttggactcgt ccggctctcg gcggcaacat catggctgaa
     2461 atcatttgta aagacaaccg cccgcagatg ggtacggttc gtccgcacgt tttcaaaaaa
     2521 ccggaagctg accccaatgc aacgggtgaa gttatcgaaa aagaagttaa agtttctgat
     2581 gctgacatga tgactcgttt cgtcgaactc attaaaatgg gcggcgaagg cgttaagatc
     2641 gaagacgctg acatcatcgt ttccggcggc cgcggcatga atggtgaaga accgttcgct
     2701 acgggcatcc tgaaagaatg cgcagacgtt ctcggcggcg ctgtcggtgc ttcccgtgcc
     2761 gcagttgacg ctggctggat cagcgctctc catcaggtcg gccagacggg taaaaccgtt
     2821 gctccgaaga tctacattgc ctgcgctatc tccggtgcta ttcagcactt ggctggtatg
     2881 tccggctctg actgcatcat cgccatcaac aaagacgaag atgctccgat cttcaaagtt
     2941 tgcgactacg gtatcgtagg cgatgtctac aaagttcttc cgatgctcac cgcagctatc
     3001 aagaaacaga aaggcctcgc ataagctact tgattgtgaa tgataaaagc actgtctctc
     3061 tgagacagtg ctttttttgt gaaatattcc gggtggaaag atggtttctc tcacttgaca
     3121 aggcccgaaa cgtgccgtat aatttacgca taccttagat ataagagaaa agctttgaaa
     3181 aggacaacac tttacagctc ctttatgaca gagagaggta cgttgctggg agtaccttat
     3241 aaagactgaa aagttaccgc cttagagctg agtttctgaa ctgagtaaga aactccggac
     3301 gccccgttac ggcgcttgag tacgctggga tttttcccgg tgaactaggg tggaaccacg
     3361 tgagtaatgc tctcgtccct tgaggacgag ggcttttttt attaaggagg atgtttcgta
     3421 tggcagaatt actgttgaaa gatggaagca aaaaacaatt ttccgatggc atttctcttg
     3481 cccaggccgt aaaagaactg agcaacagtc ttgctaaaaa agtattggtc gcaagggtaa
     3541 acggcgaagt cacggacctt cgtgaaacta tcgtcgacgg ctcgacggtc gaattcttca
     3601 cctttgccga tcaggaaggg aaggataccc ttcgccatac agcctctcat atcatggccc
     3661 aggcgatcca gcacctctat ccgggcgtca agttcgccat cggcccgtcc attgaaaacg
     3721 gtttctacta tgacctcgat acagaacacc gcttcacaca ggacgacttc ggtgccattg
     3781 aagctgaaat ggctaagatc gtaaaagaaa atcttcccat taccaaagaa atcctctccc
     3841 gtgccgacgc gttgaagatg ttcaaagatg ccggccagga ttataaagta gaactcatcc
     3901 aggaccttcc ggaagatgcc gtcatctccg tctatcgcca aggcgacttc tacgacctct
     3961 gtgccggtcc gcactgcgaa tcgacaggcc gggttaaagc ctttaagatc cagaccgtcg
     4021 ccggtgccta ctggcggggc gatgaaaaga ataagatgct ccagcgtatc tacgggacgg
     4081 cttttgaaaa gaaatccgaa ctcgacgact acttgaagat gatggaagaa gctgaaaagc
     4141 gtgaccatcg taaattgggc aaacagctcg gcctgttcat gctcagcgaa tacggcccgg
     4201 gcttcccgtt cttcctgccg aagggcatga tcgtccgcaa cctgctcatc gactactggc
     4261 gcgaagttca ccgccgctac ggctaccagc aggtcatgac tccgatgatc atgaaccgcg
     4321 aactctggga aacttccggt cactgggatc attacaaaga aaacatgtac ttcaccaaga
     4381 tcgacgatga agactatgcc atcaaaccga tgaactgccc gggcggcatg ttggtctatg
     4441 ccaacgaacc tcattcctat aaggaactgc ccctgcgcat cggcgaactg ggactcgttc
     4501 accgtcatga attgtccggc gccctccacg gcctgttccg cgtccgctgc ttcactcagg
     4561 acgatgccca catcttcatg atgcccagcc agatcgaaga cgtcatccag gaaacgattc
     4621 gcctcttcga cgaagtctat gctaccttcg gtttgaccta ccacgctgaa ctgtcgactc
     4681 gtccggacaa ctccatgggc gacgacgcta cctgggaaat gacgaccgat gccctgcgca
     4741 aagccatgga tgacttcggc ctcgactaca gcatcaacga aggcgacggc gccttctacg
     4801 gtccgaagat cgacttccac ttgaaggact ccatcggccg tacctggcag tgcggtacca
     4861 tccagctcga tatgctcctg ccggaaaaat tcaacctgac ctacaccggg gaagacggcc
     4921 agaaacaccg tccggtcatg atccaccgcg tcgtctacgg ttcgatcgaa cgcttcatcg
     4981 gcatcttgac cgaaaactat gccggcgcct tcccgacctg gctggctccg gtccagatca
     5041 agatcctgcc gatctctgaa aaacacttgg cctactgcca ggacgttgcc aagaagatgg
     5101 aagccaaagg catccgcgtc gaagtggatg aaagcaatga aaaactgggt tacaagatcc
     5161 gcaaggcaca gatggaaaaa gttccttaca tggccgtcgt cggcgataag gaaatggaaa
     5221 acggtgcctt cgg
//
LOCUS       DAWDXZ010000065         5230 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4345_length_5230_cov_8.554396, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000065 DAWDXZ010000000
VERSION     DAWDXZ010000065.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4345_length_5230_cov_8.554396"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1406 a         1247 c         1380 g         1197 t
ORIGIN      
        1 ttttccggat cttcactttc accggcggca atgccgatca tttccgtccc ttggaaggag
       61 aagccggcga ccatgaagat acccaacata gccgtgaagc cgccgacgaa gggcgcttca
      121 tcgacggtcc agttgacgaa gcccggtgac ggttcagagc cgaggcccaa gatcaagagg
      181 gtccctgtaa acaggaagat caagaccgtc aggaccttga taccggcgaa gatgaattca
      241 ctttcgccgt acgaccgggt tgacaggtag ttgagggcga ataagatgac taggaacaag
      301 ccgctccaga gggctgccgg tacgtcgggg aaccagtact tcatgatgag ggcgccggcg
      361 acgagttcgg ccgcaacggt aatggcccag ttgaaccaat agttccagcc caaggcaaag
      421 cccaaggact tgtcgacgta gcggttggca tacgtttcaa aggagccgct gaccggaagg
      481 tatgaagcca tttcacccag tcctgtcata agaaaataga ccatgatgcc gatgagggca
      541 taggcgacga gtgccccacc cggcccagca gtgctgactg tttcaccgcc ggcgacgaac
      601 aagccggtac cgattgcacc gccgatggcg atcatgttca tgtggcgggc tttcagactg
      661 cggcgcagct tttgctgctg ctgttgagtt tcttccatgc aaaatcacaa ccttttgtat
      721 taaattgata actagaatct aaaaaaactc atacgaatat attatatagg atgacagcga
      781 taatgacaaa taaatattct gcaattatgc aagaattata tagggaagcg aatcatttgt
      841 agccttttag cggcttttcg gctataatgg tagtgttatc atgcgtaatg ggaaaggata
      901 gattatggaa ttagaagaat gccgccggga gattgactta ttggaccggg acttggccaa
      961 gatactggaa cggcgcatgc aggtcgtggc tgctgtcgcc gcttataaaa aagagcatca
     1021 catggaagtc tatgatccgc gaagggagcg tcaagtgctg gataaaattt ctaatttgac
     1081 ggagcagaaa aatttggccc cttatttgcg ccgcatctac cagtgcatca tggatgaaag
     1141 caaaaaatac gaacgggaac acatggggat aggctgatgg aagaacttat tgtacgaggc
     1201 attgccgaac gaattttatt tgaggcctcc gattcggatt accgcgtctt tcgcctccat
     1261 gatgaaagcg acgatacgac ctataccgtg actggtcacg gaacgaagcc cctcgtcggc
     1321 gaccgcctgg aagtaaaagg ccattgggta cagcataagc gctatggacg acagtttgcg
     1381 gccgatggtt ggagccgcgt cattcccgaa tcggtcgacg gcattgaacg cttccttgga
     1441 agcggcgccg tcaaaggcat ggggcctgct ttggcccacc gcgtcgtcgc ccgttttggc
     1501 aaggatacga tggagatttt ggaaaaggat gctcagcgct tacttgaaat cgaaggcatc
     1561 gggcctaaaa aattggccgt cataacggaa tctttctacg aagaaaaaca gatcaacgac
     1621 atcgcctacg atttggaaca acacggcgtc gccggacgct atgccggtcg cctcctgcag
     1681 aaatacggcg acgatgtgaa atacgtcttg accgaagaac cgtatcggat gattcatgaa
     1741 atcgacggct tcggctttaa gacggccgac caaatcgcct tggcgtacgg catggaccgt
     1801 cagtcgccgc agcgccttgg tgccggcctg gcctacgtcc tccagaccat gacccaaaac
     1861 ggccacgtct gcattcccga tacggaattg gtgcggcggg cggcctttct cctgcaggtc
     1921 gatgccctgg gcctccacga tatcttacag gataccgtcg atatggggca attacggacg
     1981 gcggattttg aggggacggt ctacgtctat acgccggaag cctatgaaga ggaagaagcc
     2041 attgccggcc ggatccgcga gatgactgag atgaaacctc tttccatgaa aacgcatgtt
     2101 cagctctttc tggaccgctg gcaggattcc tgtcattttg aactggcaga caagcagcgg
     2161 gaagccgtcg aaaaatccct ccagtcgggg atgactgtca ttaccggcgg tcccgggacg
     2221 gggaagacga ccgtcgttca gaccatcatc cgcctggccg aacaagaagg cctgcgcatc
     2281 ctgctctgtg cgccgacggg gcgagctgct aagcgtctgg ctgaaacgac gcagcgcaag
     2341 gcgaagacca tccatcgact cctcattccc gacggccatg tcggcaaagt ccagtccttt
     2401 gaatacaatg aaacgaagct cttgccggcc gacttagtca tcgtcgacga agtgtccatg
     2461 ctcgatatgg aaatgatgta ccacttactg aatgccttga agccccagtg ccgctgcatc
     2521 ctcgtcggcg acgccgacca attgccgtcg gtcggagccg gtgccgtcct ccacgacgtc
     2581 atcgcctcgg aaacggtgcc tgtcgtccgc ttggatacga ttttccgcca aaaagaaggg
     2641 ggccgcatcg taaccaatgc ccaccttatc aacaacggcc gcctgccggt ggtcaacgaa
     2701 gacctggaat tccgcttcgt cgaaatcgag accgaagccg acggggcgac gaagatcagc
     2761 gccttgtacc gcagtgaagt tgaggaaaca ggtgacgaat ttgccgtcca ggtcttgtct
     2821 cccatgtaca aggatccctg cggcgtcgac aacctcaatc agctcattca ggagcggctc
     2881 aatccgcccg ttcaagggaa gggtgaattg aagggccgcc acatgatttt ccgcgtcggc
     2941 gacaaggtca tgcagaagca taatgattac gaaaaaggcg tcttcaacgg cgacatcggt
     3001 caaatctttg ccgttcagca tgacatggtc tacgttcgtt atcccgaaca ggacgtcaag
     3061 tatgaaggcg cggaaatcga cgaaatcacc ctggcctatg ccattactgt ccataagagc
     3121 caggggagtg aataccatac ggtcatcatg gccctggtca acagccattc catcatgctc
     3181 cagcgaaacc tcttctatac ggccgtcacc cgggccaaac gaaaagtcat cttagtcggc
     3241 actaaacggg ccgtccaaac ggctgtccag aaccagcgga cgagccgccg ctttaccttg
     3301 ctcatcccgc ggctccaagg agagctgctt acgtaatatt ttgcgacagg aggcattttt
     3361 atgtcactgc tcacccttat taccaacctc ttctatccgc cgacctgccc ggcctgcggc
     3421 gtcctgatga aacgggacgg cgactggtgc ccgtcgtgcc tcgctcgagt gtggcatccg
     3481 cggcgcatca accgctcccg acaattgaag gccctcgacg cctgttactg tttggccgac
     3541 tacgacgggg ccatgcgccg tctgattcat gatatcaagt acaagggcag agagggaaaa
     3601 tgccgatcct gtcgggtatt gctggaccga tttccctggc ccgaacgact gaaagccatc
     3661 gacatggtcg ttcccgtgcc cttagcaccg gagaaaatga aaagccgcgg cttcaaccaa
     3721 gtcgaaagcc ttttcaaaac atgggctgac gatcattatc gctgggccga catcctccag
     3781 cgtttgcgtc ccaccgatgc tcagtggcat cttcccagaa gtcagcgttc cgaaaacgtc
     3841 caccgcgcct tcggccttcg ggaaacagcc gatgtcaaga ataagcatat cctccttgta
     3901 gagcaaagat gcttgggatt gatacaatat tgggaacggt gtgcatagcg gaaatgtcca
     3961 tatatagcga ggttttggca aacgtcggca gatggccggc attttttatg ttctgaaatg
     4021 caggtagaat cagaaggtat cgtataagtt cttagcaaca ctgtggatat gccattaacg
     4081 atagcaatgg catcgttaat ggtatagaaa gataatttgc cgtgttatcc gacttgtttt
     4141 ttattgtttg ttagtgttgc tatggataag ataaaaaatg tagtacaata ttattatcaa
     4201 tggaaagtgt gttatcgtct ggatgaggtg ataattcatg cgggtttaca attatgcatc
     4261 ttttgctaag gtgttttcga agggcatcat caagcctaat atgactaaaa ttgctaagat
     4321 tttatttgag ccagtcatta gtcttggaga ctgtgtcaat agacatgggg aaccatatac
     4381 catcaataat aagtcggcaa gcctttggta taaacaagaa tcagacattc caggcaattt
     4441 gaaaaaggca gtgaatgatg gccgtgtata taagggaata gggaaatatt ttactgatga
     4501 agttttggat aaagtcctta gtggggttaa gattgatgat atggtggctg agttgctaaa
     4561 cctcatcaag gaatcagatt tgtctgaaat ggaaaaaggt gatttaaata aactgtatgc
     4621 gggtggtgat ttgggaggat ttttaggtag agcgttcttg tatgccatat tgcaaaacaa
     4681 tatgaaaaag gatgcatctt atgaggacta ttcgaaaaca gcccaggcaa cagccgcaga
     4741 tattaaccat gaaatagaag ctttcaggca gtttgttggg caatttaaaa agcctactcc
     4801 gctgtctcca cctttgacta ttgcaacaga agaaatgaaa tatgtaacag agctattccg
     4861 cgtttatcag gaaaaaacag gagtggaatg taattgtgta aaggatctcg attcatgccc
     4921 taatatgaaa aagaatttta acagacaaag aaaagattac tatttagcag aaactattag
     4981 gaggggactt cgagataccg tagcaccaca ggaggaggaa aattttgaag cagtaaaaga
     5041 agaaatgtat gaaggtgtcg taacgacgga agaaaaagat tattcttgtg gctatgacag
     5101 aatgaacgct gtaatggagc atgcgacatt ggttgagctt tcttctaatt tacaaactgt
     5161 gactctgaat tggattggac caggggaaaa gaaaggcatt tgtcatatgc tggtcaatga
     5221 tgagaaactc
//
LOCUS       DAWDXZ010000066         5202 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4361_length_5202_cov_8.497377, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000066 DAWDXZ010000000
VERSION     DAWDXZ010000066.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4361_length_5202_cov_8.497377"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    528..537
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3239..3248
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3674..3683
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1577 a         1051 c         1298 g         1246 t
ORIGIN      
        1 ggctcattaa tagtgatcaa gctaagacga cacaattctg gattaactta aaagaaaaat
       61 cgattaaaga aatctatcgg aaatacttgg ctgaacaaca taaatatacc agaccgtcag
      121 aagacgattt gaaaaaattc gatgcactca tggaaacatt aaaagtaaaa gaactgtcgg
      181 cattaaccaa caatgggcgg cctaactcaa tggaccgcgc cgaaaaaaac agaaaggatg
      241 cgcaggggat attttttgag ggagcagcaa aagaagcatt tgactccggg attgttaact
      301 tagaagggtt aaaagacttg tacgaatttg aatttttgga aatgtcttat acccggaatt
      361 ggtcagaaga atggtgcatc gacgcagcaa tcgaatcgct ccgtagacgg caagaacata
      421 agaaaaacgg aacctgggaa ggtaaataac atgaacaatg acgatgaatt aaaaaataag
      481 caagcggaag cattagaaga agttatgtcg ctcggtgagg ctgccgannn nnnnnnncac
      541 cctgaaaaca tggtgttccg gaagtaagcg gaactaccct aaatttgaat gctatgagtg
      601 tcgcaagagc ggcaagtatt ggttaatcac tcggtccggc atggaaagaa tggccggacc
      661 ggaacccata aatgaagaac aggagaaata aaatggacga caagttatat aatgcgctat
      721 caaaaatata tcgtaccctc atagaatgga aacggcatcc ggaaagcctg gaatggacgg
      781 ccaaagccat ggaagaaatg agcaagctaa aggaagactt cggggtcgaa ttcaacgatc
      841 ctgaattgca ccgtcaatgg caagagcgcc aagcggccga aaaaatcaag caaacggcgg
      901 ctgatacgcc agcacctatt aaaaagccct tggaagttgg cgacgtcttt ccgtgggaaa
      961 tgtccgaaga aatcagattc acgatgaatc catttccgac attattcttg agttgggaaa
     1021 agataacgga atctgagtta gttgccatag aaaacgggag actagacttc cgggtaacct
     1081 ttttcgaggg ggtgacattc gttttgacaa aattcggcga cctacggtgg atggccgccc
     1141 cgtataacat ccatctggat ggagacgtcc ccgcgcaagc gattattaac atcccggaag
     1201 ataatggatt agtactacat acattcttag tagaaaaaga agtcaataga attaaggcta
     1261 tccgggacat tgtactcccg cacggcatca gccgccggtt gattagctgc aatcaagtgc
     1321 agctggaaac agcattcaac cctcaaaagt acctcgaaaa actgaatgat atatataaga
     1381 aatttccaac atcggcgcta ttagcgatgt cccacgaacg acttatataa ttatcaatag
     1441 cgcctcgcta gtcggcatta ttgctggcta tgtggggctt atttttttta aaattattca
     1501 aaatacaccg atatcggtat tgacagtaca ccgatatcgg tgtataatgt aatcaaggaa
     1561 aggggataaa gagaaacccc agataacagt taaatctaag gaggaataaa ttatggaaag
     1621 acgggtagta gtaaaattgg acaacggaca ggtgttaggc acgatttgga cgaatcggat
     1681 gctcgacgtc gacgaagcga tggagcttct cgagatagag cccactgatt acgataagaa
     1741 attcgtggca gagggctaca aagctgaata cttttatggt cccgatggga agggattcca
     1801 tatcagcgaa gtagtatttg acgacgagga atagaaaagt tcatccggac aggtaactgg
     1861 cctgcccggt gttaaccaaa aggaggatat ataatgagct cgatcagaat agatgtaaaa
     1921 aatgataaag ccttagtata cgcgccttat aatcctgatt tccgcacctt gattagccct
     1981 atcggcaggt ggaccggaag atcatgggaa gttgccgaag gttgcatagt cgacgtcaac
     2041 cgaatgctgt ctatctgcta tggttacgat cccgataacc cggatgactt gatagatatc
     2101 gaagttacca ctaagaccaa aatagctggc gatggatacg atggaattaa catgttcggg
     2161 cggcagatcg tgtatgctat cggaccggat aacggcgcgt ggctcggtga aggaatcatc
     2221 gttatccagg gtaaggcaaa ttcaagaggc tcccgaaacg gctggaaaac tatcctctct
     2281 aaaggagcaa tatttaagat tttggacgtt ccgaaaaatg cccttgagat agacaatgac
     2341 ttcagggaaa tgattgatat caagatttta aagtatgagg gttagataaa tagcggcaaa
     2401 agatgctgga caaaaataat taatatgata taatgaagtc aagcaagggc atctcggttt
     2461 tcccgagatg tagtcacagg tcgcgaggcc tgtgcggatt gaaagatata tgttgtcgtt
     2521 gtcttgacta aactaaaaga caacaacgaa agtgtcgccg ccttatggcg gcgcgggtta
     2581 aaacatgtac gaagagagca tcatccttta tggatggtgc ttttttcgtt gacagtcaat
     2641 tacatacgtg atagactata tccatggggt ggcagtaagc cgccaaaaaa aatatcggag
     2701 cgtactacgt gatagtacaa ataaatccct actctattga gtagggattt ttagttttcc
     2761 ggaagacctg cggcgccgat cggtgtcgta gtacgtcaaa aaaaaagcat gcccggaagg
     2821 gctatcagga tgagccgacc agttaaaatg atgccggctt ttttcgcgtt attatgcaat
     2881 cgtccgtaaa gacggaaatg acttatatcg gctttgaccg atggtgtcct attagggtgg
     2941 ctacacggtg gctacatata gccgcccatt atatgccgat aaatgcttga ttattggcta
     3001 aaattatcta aagcgcatta catgctacag aatggacaaa tattcattgg tgtctgtgtc
     3061 agaatttttt ttaatacaat tttttgcatt aaatcagcag tcgtggccaa tctctgcgaa
     3121 tcatgataca ataaaaaaca tacaagcccg agacttttta ggaaacggtg atgagaggca
     3181 tcatcagaaa aacgggtaaa aaggggacga atatgaccga agacagagat aagtatcgnn
     3241 nnnnnnnntg gacaatttgg cgaagccccc cggaagttta gggaccttag aaaaacaggc
     3301 caagcaggtg ctcctggcct gggggcagtt tcataaagag cttcggccga agcatcttat
     3361 ttttgcggcc gacaacggtg tcgttcattc cggtgtcgtc gcacaattag ctgatattac
     3421 ctatatgcag agccggcaca tggtagacgg cacgtcggcc gtgacctgct tttgccgcag
     3481 tcagcagatt ccctacgccg tcatcgacgt cggcatcgat agtgaagatg ccgtcggcat
     3541 cgatcgcaag atagcccggg ggacgcggga tttttccaag gagccggcca tgagtgaagc
     3601 tcagcttcaa caggccctgg cggccggtcg ggaacaggta cagcaggcta aggccgaagg
     3661 aatcaatctc ttgnnnnnnn nnnggtaata cgacgacgtc ggctgctgtg ctgacggcct
     3721 tatcgccgaa aaaaatgacc tggctttccg ggttcggttc ggccaggggg aattacaagc
     3781 tgctgtttca caagcgccgt gtcattgccg aggccatgaa gcgctatgaa gggcagttgt
     3841 cgtcaccgga agcggccctg cgctgtgtcg gcggattcga tctggcggcc ctgtgcggcg
     3901 ctatgctggc ttgtgccgat gagagaatcc ctttttatat cgacggcttt attacggcga
     3961 cggccctggc ctgtgccgtt gccatggatt ctcgggttcg ggattatgcc ctgccctctc
     4021 atatttccag ggagcccggc atggctcagg ccttgcgcct atgcggtatc gacgagtatg
     4081 acgtgcctct tacggccggc ctttccttgg gagaagggac gggggccgtc ttgggcgtta
     4141 tcctgctcaa gacgatgatg tacgccgttt ggcatatggc caccttagac ggcatcaatc
     4201 aggaagctca ggaacgatat gataaacgcc aaggagatgc gcaccatgga tgacgtaacg
     4261 agagaaaagg cccgggctgt ctttgcccga ttaggcctga cagaagaaca agcgatagaa
     4321 cttttttata agcggaccat tgaggtcgga cggatgcctt ttgctgcgtc tgaggaaaaa
     4381 acgcggaaga tgaagaaaaa gcggatgaac gagcgttttg ccgaatggga tgctttctaa
     4441 tttgtttaca tgttgttcag cagaaagcca acgattttaa ttgttggatg aatgattgta
     4501 gatattttat tttattttat ctacggatat gctatgatat aaatatggaa acgacatatt
     4561 ttagaacaaa aactaccgtg tcattcatta actatcattt tgtattctgc ccaaagtacc
     4621 gacgtaaaat atttcttatt ccacgggtgg aagaacgttt caaagaacta actcgacaga
     4681 aatgcgagga actcaaaatc gaaattctcg cgatggagtg ccatattgac catgttcatc
     4741 tttttttgaa atgctggccg aagcagtcgc ctgccgaaat tatgaaggag ataaaaggag
     4801 cttcttctca tgttcttcgt aacgaatttc caaaactgaa agggatgtcc agcctttgga
     4861 caagaagtta tttcgtttcg actgctggca atgttagcag cgatacgatc aagtggtata
     4921 ttgatacgca gaaaacaagg gggtgagttg caatgataaa gacctacaag tttaagcttt
     4981 atgccgcaaa acgcaataaa aaacttcatc ggcagattaa tgccgcaggt cttgcctaca
     5041 atcattgcat cgccctccat aagcggtact ggcagctata tcataagttg ctaaacaaat
     5101 ttgctttaca aaaacactta actcagctca aaaaaattga gcggttcagt tatctcaaag
     5161 aaataggttc acaggcattg caggatgtca ctgaccgcat cg
//
LOCUS       DAWDXZ010000067         5189 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4369_length_5189_cov_10.701208, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000067 DAWDXZ010000000
VERSION     DAWDXZ010000067.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4369_length_5189_cov_10.701208"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1261 a         1301 c         1377 g         1250 t
ORIGIN      
        1 gcgttctcag acggaagctc gtgtcgagag agaccgtaaa gtcttttaaa ttccgattgt
       61 tgacgccgat gatgcgggca ccggccttga tggcccgaag gacctccgtt tcgtcgtgag
      121 cttcaaccaa ggccgaaagg cccagagaat cggcaagttc aaagaagcat ttcaatcgtt
      181 catcatcgag gatggcgcag attaaaagga cggccgaagc accaatggcc cgggcttcat
      241 aaatctgata ctcatcgatg atgaaatctt tgcgaaggac cggcgtctga accgcctcgg
      301 caatatcctg taaatacttg ggacttccta agaagtaatc ccgttcagtc aatacggaaa
      361 tagccgccgc accggcctgt tcatattccc tcgcaatggc ttcataggga aaatcaggag
      421 caatcagtcc ttttgaaggc gaggctttct tgacttcaca aatgaaggaa agacccttct
      481 gtttcaggtt atgttcaaag gggaacccct cttttttcgg agctgccagg gcccgttctt
      541 tcatctccga taaggaaact cgtgccttat cctctaccag cctttttgca acagctgccg
      601 ttaaatcatc gagtatcatg tgctcacctt cttacgagtt gctgacagca atgcagcgat
      661 tcagcgtttc catagccttg ccgctgtcga tgagatcggc agccattttg ataccggcac
      721 cgatggaatc ggctttaccg gcaatgaaca aggccgttcc ggcattcaag aggacggcgt
      781 ttcgtttcgg cccctgaaca gttcccttca ggatgcctcg cgtgatgtcg gcattttctt
      841 ccggcgtgcc gccgacgagg tcttccttcg ttcccggcac catgccgaag tcttccggcc
      901 gcaggataga cgtccgataa tatccgtcac ggagttcaca aatcgtcgtc ggcccgttcg
      961 gcgaaatttc atcgagtttt tcctggccgt aggcgacgat tcctcgtttg acaccaaggg
     1021 tcgacaatac tttcgccagg ggttcgacca ggtattcatc gtaaacgccg aggaggatca
     1081 tcgtcggctt agccgggctg gtcaagggcc cgaggatgtt gaatacggtc cggaatccca
     1141 gttccttgcg gatgggtccg atatacttca tagcggcatg atatttctga gcaaagagga
     1201 agcagaagtt cgtttcttcc agcatcttaa gtgccagttt cggatcctgt tcgatattga
     1261 gccccagtgt ttccaggcag tctgccgtac cgcacagaga cgaggcggct cgattgccgt
     1321 gtttggctac tttaacaccg gccgaggcaa taaccatggc cgacgtcgtc gaaatattga
     1381 aagaatgggc accgtcgccg ccggtaccga cgacttccag gacatccatg cccggatgag
     1441 gaacgggcgt tgccatagag cgcatggctt cggcacaacc tgaaatttca tcaatcgttt
     1501 cggccttggt gctcttcgtc gacagcgccg ccaagaaagc ggcattctgg gtcggtgtcg
     1561 tcttgccgtt catgatttcc agcataactg cctttgcttc gtcataggtc aaatcacctt
     1621 tatttacaat cttgataatc gcatctttaa tcatcgtcgt tacctcctcg taaggactct
     1681 atctattata attttaagaa attttcgacc atcatcggtc ccgtcggcgt caatatcgat
     1741 tccgggtgga attgcaagcc gtatgtagga taatctttat gggcgacggc catgatttcg
     1801 ccgtcatcgg tccgagccag gacacgaaga tcatcgggaa gggtatcggg atcgagggcc
     1861 agcgaatgat accggccgac gtcgaccgtt tcgggaagtc ccttaaagag tggcgtttcc
     1921 cggtcgaggg cggcgacgga ttgtttgccg tgcatcaatt ccccggcgta ggaaatggtg
     1981 ccgccgtaga cttcacaaat agcctgatgg cccaagcaga cgccgaggat agggatcttt
     2041 ccctcaaaat agcggatggc ctgttcacaa atcccggcat ccgacggccg acccgggccc
     2101 ggcgaaatga tcagatgatc cggatgcagg ccctcaaggg cttctacggt catttcatcg
     2161 ttgcggatga ccttaatgtc cggattcaac tgtccgatga gctggtagag attataagaa
     2221 aaactgtcgt aattatcgat gagtacaatc atttgtcaat ccctccattg gcccgctgca
     2281 gggcgatgac gacggccttg gctttattca ggcattcctg gtgttcctgt tccggtacgc
     2341 tgtcggcgac gatgccggca ccggagcagg cgtagacgtg attccccttc ttataggcca
     2401 ggcggatgcc gatacagcaa tccatattgc cggtaaaatc gatgtagccg acggcgccgc
     2461 cgtaaacgcc gcgcttatgg ccttccagtt catcgatgat ttcgcaggcc cggattttcg
     2521 gagcaccgga caaggtgccg gccggcaagg ccgcggcaat ggcatcgacg gcagtcttat
     2581 cgtcccttat cgtactgtgt acggtagaac cgatatgcat gatatgagaa aagcgcagga
     2641 cgttgagata acgttcgaca tggacgctgc caaagtcact gatgcgtccc aagtcgtttc
     2701 ggcctaagtc gacgagcata ttatgctctg ccaattcctt ggggttgtgc aggagttcgt
     2761 cttccagttg attatcttct tccaccgtct tgccccgcgg cctcgatccg gctaagggaa
     2821 aggtaaagag gtcccgccct tcgaggcgga tcagcgtttc cggagaagaa ccggcaagct
     2881 cgatatcgtc gccggagaag aagaacatat acggcgacgg gttgatcgtc cgcagcacgc
     2941 ggtacgtatc gaggaggctg ccttcggctt cagcctgaag gcgattggct aagacgactt
     3001 ggaagatgtc cccttcgcgg atataatgtt tccccttttc aaccatttgg cagtattcct
     3061 gctcattaaa gaggggttta aagtcgctct ttaactgaag gggctgggcc ggtgccttct
     3121 cgccatggcg aatgaggtct tccatacgct gtagttccaa catagcccga ttataatttt
     3181 cttctgcccc gtcgaggcga acgttgacga tgaggtaaat ctgctgcttt aaattatcga
     3241 agacgatgac cttgtcgaag agcatgaggt cgaaatcatt aaagccatcg tcatcagcgg
     3301 ccttgagtgt cagtgacggt tcagcatatt taataaaatc gtaggaaaag tagcctacca
     3361 acccgccggt aaagggaggt aacccttcca ggcgcggcgc cttattgtcg gctaaaattt
     3421 gagaaatgag ctgttccgga tggtccgtat gaatctgtac ttcagccccg gtcttgattg
     3481 taaccgttcc gtctttgcag aacaggccca tctgaggatc gtatcctatg aaggtgtatc
     3541 ggccccaacg ctgagaatct tccatacttt ccaagatata gcaatgagag ctgatatgct
     3601 tgaccgttcg caagacttca atcggggtac aactgtcggc atacatgctg actccgacag
     3661 gaacgacctt atattggcct tggtaggctt ttacctgttc taagctgagc gtttccatac
     3721 aaacctccta aaaaaataat ccgtccatga caatacgatt tgtcaaggac ggatttaagt
     3781 tccgcggtgc caccttgttt taggggttga gccctactct cgacggaata ccaacatatt
     3841 cccggtaact gacgtatacc aacacgtcgc aggatactca gccttgcggc ctttgactgc
     3901 gccctcagcg gtccatttaa cgagctgcgt cggccggctc tcagcacccc ggctctctgt
     3961 acgtgcacgt catcgttttt acttccgcct caatggtttc atggacgatg ttcaattgat
     4021 gtttgtatga tacaccgcat gatttaaact gtcaaggtag tttttatata atttttaaga
     4081 taatcgtttt acttgtgttt aagcccacac ttttagggag aaatttccgt cttacatcat
     4141 ggcaatgatc ggcgagatga tcaagaggat accggcgaat acgatgagta ccagcgaaat
     4201 tcccttatat ttgtgaaggg cattgacttt gtaaaccaag tacgccggga taaggcaggc
     4261 gatgataccg aagatcggtc cgagaagggt caggagcttc aagaccggtg cattgaggac
     4321 gatggcgccc caggcaacgg caacggcgaa gatgaggatg ccgaagctga ccattttctt
     4381 attgatcttg tcttccggca tgaagcggtg caggagattc atggcgatcc cctggcaggc
     4441 ttcgcggaat ccgaggaaca cactgaagaa ggccgtcata acggcgaaga cgttgaggac
     4501 caggctgaag atcttaacag cgctgccgtc gaagcccttg gcaacgatag ccagtgcaga
     4561 aatgttctgg gaataagcga gaacggcttg atcgtgaccg agcgccaggt taaaggacat
     4621 ggcatacgag aagatcgtaa tgaacaagac ggagaaagcg attttcatag cccgcatggc
     4681 tttgtagcga gcgacgtgga tcgatttgtt atgagagcgg taagaaatga ccatggggct
     4741 gatgctgggg ataaacagaa tcgaggtcaa ggttacaggc agcatggcaa tcgtgttttt
     4801 cacgaggtag cctaaatcgg ggaaaacgcc gatattggca atgttccagt gctggatcat
     4861 gatgccgcct aaacagatga cgactaacaa tttcgttaaa accatcaatg tcgaaatccg
     4921 gaacaagagc tgttcaccac gggaagcgag aaggaccatg gcacagatga tagccagccc
     4981 ataaaaagga ttgtcggaca ggagcgtatc ggtgacgccg aaagaataga ggaaggatgc
     5041 actgtcattg gtcagtgccg tcgaatacat gaaaacacag atgagaatac tgaggaagta
     5101 aaggatgccc agcaggaaac cccagttttt gccgagatag ccgctgataa cgctgggata
     5161 gtcttcacac cgcggcgaat cggccaggg
//
LOCUS       DAWDXZ010000068         5159 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4396_length_5159_cov_7.649099, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000068 DAWDXZ010000000
VERSION     DAWDXZ010000068.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4396_length_5159_cov_7.649099"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2870..2879
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3801..3810
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4672..4681
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1247 a         1395 c         1313 g         1174 t
ORIGIN      
        1 aacggcagtc ggtgccttgt ccggctcttc cttgggagcc tgatcaagag cttgaaaatc
       61 gaaaatcttc cgcttttcat gccattggcg cagggactgg gaggcatcct gcaggccttc
      121 gacggctttt ccggaaacgc gggccgcttc ctggccggcc ttatcggcta cgacggctac
      181 aggttgagaa atcgaccaat tcttccaaat cagaaaggta acgatagccg ctcccacaat
      241 ggccataaag gccccaaaaa agccgagaaa ctgctgcagc accgaggata cgacggcccc
      301 gatgatacct cctccgacag acaagctgga cggcaggaat tccgtccctt ccggaatgac
      361 caacaaatga ataagcccca aaaggagcac ataaatccaa gtgccgatga gccaggaccg
      421 cgatacgggg caggccttgc gaacgacaat atacttcagc cccagaacga ccaaagacag
      481 gctgacaaca atccgtccca agccaaagat atacgataag acattgtcca cattatgtcc
      541 gatacgaccg gtatcgatgc ccataaaccc gacggtcgcg aagacgccga ataagatgca
      601 gaaaataccg ataatctcat attttaaaag gggcgacttc tctgcccgtt tggcagtccg
      661 agtccgccgg gtcgtccgct tgcggcgggg gcttttaggg gtccttccgt ttgccaaaat
      721 agttcacctt ccactaatag attacttgtc actgcggcgt ttatgccagg cagcggccat
      781 atctttttca tggccataag gtctttcctg ataatacgac gcacctttca gggcctccgg
      841 caaatactgc tgatcgaccc aggcgccgtc aaaatcgtga ggatactgat aatttatacc
      901 gtgccccaac ttggcggctc cgctgtaatg actgtcccgc aaatgagccg gaatgtcgcc
      961 gcagtcttta cggcgcacat cgctgagggc cgcatcgaca gcaaggtaag cgctgttgct
     1021 cttgggtgca caggcaacat agatgacggc ttccgacaag atgatccgtc cttcaggcca
     1081 gccgacaaat tgaacggctt gggccgctgc attggcgatg accaaggcct ggggatcggc
     1141 aaggccgacg tcttcagcag cacagatgac caggcggcgg gcaataaagg acggctgctc
     1201 gccggcttcg atcatgcggg ccaggtaatg cagggccgca tcgggatcag aaccgcgcat
     1261 gctttttata aaggccgata ccgtatcata atgggcatcg cctttcttat cataggtata
     1321 caccttatga ccggctactt tttcgatggc ttcctgtgtc aacttcccgc cgacaggaac
     1381 gaccatggca acctgttcca acagattaag gccgacgcgg gcatcgcgat cagccaggcg
     1441 ggcaatggtt cccagcattt cgtcgctgcc ttcaatgccg aggtttccca atcccttcac
     1501 cgtatcggtc agggccctgc gcagcacaga cacgacggcc tgttctgaca agggctgaag
     1561 ggaaatgacc cgcagccggg ataataaagg tcggttgact tcaaaataag gattttccgt
     1621 cgtggccccg accaaaatga ccgtcccgtc ttcgacataa ggcaggagca cgtcttgctg
     1681 actcttattg aagcggtgga tttcatcgat aaagagaatc gtccgctttt gatataaatg
     1741 aaccgcttcc ttggcagctg caatcacttt tcgcagctct gccgtcccgg cggttacggc
     1801 attcaaggtc acaaagcggc tgtccgtttc cgttgcgatg acatgggcca gcgtcgtctt
     1861 accgacgccg gacgggccat agaacaagag cgacggaatc gtatcccgtt cgatcatcat
     1921 ccgaaggaaa ctccccggac ctatcaattc atcctgtcca tagacgtcat ccaatgattc
     1981 aggccgcatg cggacggcca gcggcgcata ctccctgccg ctccgttcat cggccatgga
     2041 aaataaatcg tctccgctca tttgtttgct ctccagacta aatagaacgc cgccaagagg
     2101 ttcttagcgg cggaaacata catacattcg tgaacaagaa aagagcccca ccatgccgtt
     2161 tataaaccga gttttgagcc tgcctaagca ggtgggtgtc ctcctatatg ggtcgacagt
     2221 ccagtgaagg cattgcctcg caatatagag accgaactcc caatgtaaaa agtgttggtt
     2281 caaaacatcg agaccattta cgtacacagt agggtttctt cttgtacata gtataataaa
     2341 atactcaaga aaaatcaagc cttgacaatg tgaaaagggg ctgctgccca tacagcatcc
     2401 ccttttcaag accttagcag ggcccggaag gacctgttaa cgactttatg acaccgatga
     2461 aaatcggtct tattttacaa cctttttaac cgtttcgacg acattttctg tcgtgaagcc
     2521 gaattttttg aacagtgtgc ctgccggggc cgaagcaccg aaggacttca tggaaaccgt
     2581 agcaccgtcg aggccgacgt atttgcccca accgaaatcg gaagcggctt ctacagcgac
     2641 acgggcccgt acggctttcg ggagtacctg ttctttatag gcttcatcct gctgttcgaa
     2701 gagttcgagg cagggcatgc tgacgacgcg gacatcgata ccggccttag ccagttctgc
     2761 tttagcgttg acagccaatt caacttcaga gccgctggcc atgatgatgg catcgggaac
     2821 ggctttcgaa gcgtcggcaa tgacgtaagc acccttcaaa gcatctttan nnnnnnnnnc
     2881 atctttactg ctgccggaaa gctgcggcag gttctgacgc gtgaggacca aagccgtcgg
     2941 cgtcttctgg ctggtaacag ccaaatacca accggcagcc gtttccgtag catcggccgg
     3001 acggaagacg ttgacgttgg gaatggagcg cagcatagcc agctgttcaa tcggttcatg
     3061 tgtcgggccg tcttcgccga cgccgatgct gtcgtgggtc aggacgtacg tcgtcggaag
     3121 tcccatgagg gcagccaagc ggatcatcgg tttcatgtaa tcactgaata cgaagaaggt
     3181 accggcatag gggcgaacgc cgccgtggag ggcaagaccg ttggtcatgg cacccatagc
     3241 cagttcgcgg acaccgaagt ggatgttgcg gccgctgtaa tcggctttgc tgaaggaacc
     3301 ttcgcccttc atttccgtct tgttcgacgg agccaggtca gcactgccgc caaagagctg
     3361 cggcatacga tctttcaagc ggttgatcat cgtgccggac aggctgcgcg ttgcctgcgg
     3421 tttatcttca taagcccaga attcttcatc gtttaagagg gcgttggcat cgatatcgga
     3481 atggtaagca tcccatttag ccttcatatc cgggaaggct gcagcgtaag cgtcgaacag
     3541 tttctgccaa tcactttcag cagctgctgc ttttttgctc agttcttcat aatgtgcata
     3601 gacttcgtcg ggaacgtaga aggtttcttc cggcttcggc cagttgaggt ttttcttcat
     3661 agcgacgacg ttttcttcgc cgagaggcga gccatgagca tcggcagaac cctgtttagc
     3721 cgggcagccg taaccgatct gggtcttaat ggtaatgaac gacggatgtt ctttatcggc
     3781 ttttgcttct tcgatagctt nnnnnnnnnn tgccttccat caggcagccg tcgccgcaaa
     3841 gagcaaaggt gtagtggtcg acgacgggat agtccggttt attgaaaaca gccgccaaat
     3901 gagcttcggc catagccatg ccgacagcca tgccaaggcc tgcaccgaga ggacccgtcg
     3961 tagcttcaac gccggtcgta tgggcatatt ccggatggcc cggtgtcaag gagccgtctt
     4021 gacggaactg cttaatatca tccagggtaa ggccgtagcc atacaagtga agcagcgaat
     4081 acagcagcat cgagccgtgg cctgctgaga ggataaagcg gtcccggttc gcccatttcg
     4141 ggtcttttgc attgtggttc atgtgatttg cccaaagttc ataggcaatc ggagcagccc
     4201 ccaagggcag tccgggatgg ccggaatttg ccttttggat tgcatccgcc gacaatacac
     4261 gaatggcatt tacacaagtc gtatcaatgg tgctcattag tcaaatcgct cctttttagg
     4321 ttcgttaaat ataaataaac atttacactt tattatgtaa aaaaaggact gtcctgtcaa
     4381 tggcaaaatg tcttttcgaa tatgaattca ttcgcttggc tgtcatttag gatagccaaa
     4441 aagaattatg ctataataaa aaggcctgta tatttcgcat agaaaggata ggatcatgac
     4501 taaaaaatac tttttctttg acattgacgg gaccctgggt cttggaattt caagcatcat
     4561 tccggctgac agcctctatt gcctgcgccg cctcatgaag gccggccatt ttgtatccat
     4621 cgcctcggga agattacagc acgatgctca gacctttgcc gaccgctatg gnnnnnnnnn
     4681 nccggccgtc gtagccgacg gcggcaacag cttgtcctta aacggagacg tgttcgaaat
     4741 gaccggcctt ccccttgaaa actgcaaatc ccttttacac gaattggatc ggcgcggctt
     4801 tgcctgggcc gtcgtcaccg acaacacgat ccatcgttac accccctatg agacctttcc
     4861 tcacgccgac ccgcgcaact atatgaaaac cgtcgtcaaa cccatcgata tcgacgagtt
     4921 gacgacggtc tataaaatca tgtacgcccg gccggagaaa aaagatgatg aacccgattc
     4981 atacggtctg cctcacttgg aatatatcga ccatacatac ctcatcgaac cgacggataa
     5041 gcgccgcggc atcctgcgca tgctgtcccg cgtcggcgga aagccggagg acgccgtcgt
     5101 attcggtgac ggattcaacg acatcaccat gttcgaaaag ccattcttcg ccatcgcca
//
LOCUS       DAWDXZ010000069         5137 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4415_length_5137_cov_8.052932, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000069 DAWDXZ010000000
VERSION     DAWDXZ010000069.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5137)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4415_length_5137_cov_8.052932"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2047..2056
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2925..2934
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1184 a         1271 c         1359 g         1303 t
ORIGIN      
        1 ttcatgaata ggttcctcct ctaattaaag cagttgtatg attttttcag ccgccccttc
       61 atcggtgacc agcgttcccg tatggcaggc ccgcatgacg ccgacaatgg cttcagcttt
      121 gctagcaccg ccggctacgg cgatgacgtg agggtgatat ttaatttctc gcagggaaat
      181 gccgatatta ttcgtcccgt acaaaatctt gccgtcgagg ccgcagtagc agcccagcga
      241 ttccccacag gcaccgaggc tgtgcagggc atcgcgctgg agtgtcgtca ttcccctttt
      301 tcgggccatg cggtcggcgc ggccaatgcc gaagatgacg acctgcgccc gggccgtcat
      361 ggcgacgttt tcctgggtct ggcggtcggc cttcagcatc atctgcaagg cagaatcact
      421 gagtccttcg ggaatatgaa tcatacggta tgaactgtgt gtcttggcag cgacattggc
      481 agcaatatag ttggcctgta cttcgacgtg gttccccatg ctgccccgag ccggcaaaat
      541 ggtgacattt gccaggcatt ccggcagcat ccgggccact tcagccatgg tcgatccgcc
      601 gctgatggcg acggtgtcga cgctgccaaa aagatcgttt aagagcaggg caccgcggcg
      661 ccctaattct tccttcgccg tcttatcact gtcgctgtca ccgggaacga tgtagacatt
      721 cttcagaccc agcttctgcc ggatttgacg ctggatatcg tcgaggttat tgagacgcag
      781 aaagcattcc aagagatccg gcatgagccg ctttccttcc ggttccatgg caatgcccaa
      841 ggtcgtaaag cggacgatgc cgctgcggcg cagaacgtcg acgtgagacc ggacgacgcg
      901 ttcagaaagc ccggaaaggc cggctaaggt ccggcgtcct accggttcgg catcggcaat
      961 atgccgtaac agtaaatatc gttccttaat ggtccccata acatcgggag ctattttttg
     1021 aaagatactc agcgtatctt gttccatctt actctctccc ttcgaaatgc ggcagaacga
     1081 cggagatcat cactgatcga tcaggctttt gcctgtcatt tcactgggaa tatccattcc
     1141 ggccaaggtc agcatcgtcg gagcgacatc ggctaagatg ccgtcgtgga gatgaacatt
     1201 tttgtgttct tcactgacca agatgcacgg tacatgattg gtcgtatgag ccgtataagg
     1261 gctgcccgtt tcgggatcag ccattttttc agcattgccg tggtccgcaa tgatgagcag
     1321 ctgtccatcg acagcatgga gggcttcgac gattttgccg acgcaggtat ccaccgtttc
     1381 aacggctttg acagccgctt caaaaacacc cgtatggccg accatgtcgg cgttagcgaa
     1441 gttgagcatg atcatatcgt atttatcggc cttgatctgt tcgatgactt tttccgtaac
     1501 gataggagca ttcatttccg gctgcaggtc ataggttgca accttcgggg acggtaccaa
     1561 gatacggtct tcgcccttgt tcggttcttc ttcgccgccg ttaaagaaat acgtgacgtg
     1621 agcgtatttt tccgtttctg caatgcgaag ctgggtcttg ccggctttgg ccaggacttc
     1681 acccaaggta tggggaatcc gttctttgtc gtagacgaca tgggccggaa gatcatcttc
     1741 gtagcgggtc atggtcgcaa agtagacgtc caattttttc ggacgcggga attcagcaaa
     1801 gtcaggaacg acgaaagccg tcgtcagctg acgagcccgg tcagggcgga aattgaagaa
     1861 gacgacgctg tccccatctt caacgggatg tttacggatg accgtcggga cgacgaattc
     1921 atcggaaacg tcggcttcat aagaatgttt caagcacgtg gctgcatctg gttcctgcgg
     1981 tccttctgcc aaggctacgg cattgtaagc tttttcaacg cgttcccagc gtttatcgcg
     2041 gtccatnnnn nnnnnncggt ccatggcgta ataacgaccg ctgatggtgg cgatttcgcc
     2101 gacgccgagt tcggcgcatt tatcttccag ttccttcaag aattcaccgg cactgcgggg
     2161 cagtacgtcg cggccgtcga ggaaggcatg gacatagact tttttcaagc cgaagcgttt
     2221 ggccatttca aggaggccga agaggtgctg ttcactgctg tgaacgccgc cgggagagac
     2281 gaggcccaat aaatggaggg ctgaatcgtt tttcttggcg ttttccatgg caccgaccaa
     2341 ggccggtttt tcaaagaagg gaccttcttt gatgtctttc gtgattttgg ttaaagcttg
     2401 gtatacgatg cggccggcac cgatattgag gtggccgact tcggagttgc cgatctggcc
     2461 gtccggcaag ccgacatctt caccagaagc accgaggaag gaatggggat attcttccca
     2521 aagtttcggc aaaaccttga ggttcgcctg ggctacggca ttatcttttt ggatatcgct
     2581 gcagccaaaa ccatccatga tgattaacat tactggtttt ttatttgcca ttgtagtgtt
     2641 ttactccttt ttgtagtaaa gaaaaacaaa aagggctgtc gccgcagcga cagccccttt
     2701 cagcgctttt cgatgtttca atctgatttg taacgggagg ctgtttaccc gttgggaaat
     2761 gcgtcgctac aaattagtgt ttgctgttgt aatcgttcat tacggcaatg aggttgagga
     2821 ccttgttgga gtaaccgatt tcattgtcgt accaggaaac aactttaacg aaagtatccg
     2881 taagggcgat acctgcttta gcatcgaaga tagaggtaag cgggnnnnnn nnnnacgtct
     2941 tcttcagtgt agccgaggat gcctttcaat tcgccttcgg aagctttttt catagctgcg
     3001 cagatttcat cgtacgtagc cggttttgcg aggttaaccg tgaggtcgac gacagaaacg
     3061 tcgagggtcg gaacacgcat ggacataccg gtcaatttgc cattcaattc aggaatgact
     3121 ttgcctacag ctttagcagc acccgtgctg gacggaatga tgttgccggc agctgcacgg
     3181 ccaccgcgcc agtctttcat ggaaggaccg tcaacggttt tctgagtagc cgtcgtggag
     3241 tgaacggtcg tcatgaggcc gtctttgatg ccgaaagcat cgttgaggac tttagcgatc
     3301 ggagccaggc agttcgtcgt gcaagatgcg ttggagacga tggtctggcc tttgtatgtg
     3361 tcggtgttaa cgccgcaaac gaacatgtct gcctgacggc cgtcttcacc ctgtttggaa
     3421 ggagcggaga gaacaacata tttagcacct gctgccaagt gtttttcagc gaggtccatc
     3481 gtgaggaaac gaccagtcga ttcaacgaca tattcagcgc cgacttcgtc ccatttgagg
     3541 ttagccggat ccatttcagc cgttacgcgg atttttttgc cgttgacgat gagctggcta
     3601 tttttggcgt caacttcaat cgtgccatcg aagcgaccat gcatggtgtc gtatttgagc
     3661 atgtatgcca ggtattcaac cgggccgagg tcgttgatgc ctacgatttc aatatcatcg
     3721 cgagtctgag ctgcgcggaa aacaaaacga ccaatacgac cgaaaccatt aataccaact
     3781 ttaatcattg tcgatacact ccttttaaaa acgaaaataa cttttgtata taattctaag
     3841 aacttaaagg ccttgctact tacaatgtaa gtatgccaca tcgggccgaa ttttgcaata
     3901 ccatggacgc tttacgtccc aatttattgc attcccaaag atgcagcagc gatgccgacg
     3961 tattccttga tggccagaca ggaattgagg agataggccg attgcggcac gacagtatag
     4021 ggcgaccaaa tcggattcat attgctgcgg tagtcagacg gatagggaat gaagtccatt
     4081 ccttcacggc ggaagaaccg agctgcccgc ggcatatgga aggcgctggt cacgacgatg
     4141 ggcttcttcc agccctgctg acggcaaatt tctttagaaa aagcagcgtt ttccgccgta
     4201 tttcgacttt tatcgtccat aaaaattcgt ggttcgggaa cgccaaggga caggagcatc
     4261 cgcttttcaa tctgcgattc attggcctca ccttcgagga cggttccacc ggataacagg
     4321 atgggcagat tctttgcttt ttccaagcga agggctgtca agaatcgatt ggccgccgca
     4381 cttccgactt gaccggtgcc gtcgacatca gggacaccgg cacgggagcc gccgccaagt
     4441 acgatgatga cgtcgccgtc gagagcttcc agggacggtt gttcataggc catttccaaa
     4501 ggttggatga ggatgtctga atcgggctcc gtcgacaaca ggtagaagac ggccgtaatg
     4561 atcagcatgg cccaccggcc gctcgccttc tttcgaaaaa gccaggccgt caaaatcata
     4621 aaggccaata cgatactgcc tatgggcaaa atccaagcgt aaatccattt aataagatac
     4681 aacatgtacg tgacaccttc ttacgactct gctgcgagtc tgttcgatga gaggcactat
     4741 cagaaaaagg gagatgcccc atcgtcgcta ctattatatc ataagacccg ttgaagacca
     4801 gttggcaatt tgtaaaatat atgttatact taatttgaat ctctatgggg gttctccatc
     4861 catggggatg taaaggtttc gacgtggatg gttgtatcat gaatagcgag ccggggtttc
     4921 atctgcccgt aaatcggtga accttttaaa ttaaacgcaa aagaaaacaa ttttgcttta
     4981 gctgcctaag ggcggctaac gtctcttgct gctaatcctg cgggcagttt gcagacgtca
     5041 cttagcggga taccacatgg ttttgcctgg cagccgtgtc gggaaactca caggctggaa
     5101 tcgtctagtt tgtccttgta cgaaaacggt tcaagat
//
LOCUS       DAWDXZ010000070         5125 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4425_length_5125_cov_10.255424, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000070 DAWDXZ010000000
VERSION     DAWDXZ010000070.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5125)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5125
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4425_length_5125_cov_10.255424"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2116..2125
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1055 a         1238 c         1517 g         1305 t
ORIGIN      
        1 atgcttctga atggtaggcc gataggaagg catctccgat gaggaccgat atctcttcat
       61 cccagacggc atcgaagggt ttactgtctt tcagcctggc ttcggttaca tgacgggttt
      121 caatgggtcc ttcgcggggc ttgatgagat atgaaatgcc atggacgttc ccgtcactaa
      181 tccaggccaa ggccagccgg caaatactat ttggattgtc gtttgccaga tctgcggcaa
      241 tggcaatcga tgaattgatc tctcgccgcc gcagggattc cagcgccttt tgggtattgc
      301 tgttcatgac gggcaacctc cgtgagtgaa gctttcttag tggccgcgtt gtttatagta
      361 cgggcggaag gtgcggcgct gccgcttcgg tgtctgttta agcttgaagt agttgtgttt
      421 tacggtgcgg tttttttcgt ccgtaaacgt attgacttca aagagattca acgattcgat
      481 gaagggaacg aatttcttgt acccgaagtt gcggacgtcg aagtcggaga actgtttgct
      541 caggagattc cccaaggtgc cggagaaaat ccagccgttg tcgtcggagt tttcttcggt
      601 cagtttgatg agggcctggc ggatggttga gaagtctttt ccgggcgtct tgccggaaga
      661 aggagtctgt tctgacggag ccggagtatg ttcgctttcc gtttcactgg gctgggtttc
      721 cacaggatga gtttcagctt cggctttttc cggctttgca gcatccgctt ccggggccgt
      781 ttcttcatgt tcggcctgtt cgatatcgtc agcatcgtgt tcttcggctt cttcccaaag
      841 gacgtcgagg tagagaaact tgttgcaggc tgagataaag gagcggggcg tcttgctttc
      901 gcccatgcct aagacgtact tttgcgattc ccgaaggcgt gatgccaaac gggtgaagtc
      961 actgtccgat gatacgatgc aaaatccgtc gagattgctc tggtacaata aatccatggt
     1021 gtcgatgatg agggccgagt ccgatgaatt tttgcggatc gtattgctgt actgctggat
     1081 gggctggatg gcgttgtcga gaatcgtgtt gcgccatgaa gccatctgcg gcgtcgtcca
     1141 gttgccgtaa attcgtttgt agacgacgtt gccaaggtta ttggcttccg aaaggatgac
     1201 gtcgatatat ttactggaaa tattttcggc gtcgatgaga acggcgagtt tcatttcttg
     1261 attcatagtt gttttacctc tgttttcatt acgtttcatc aatacatgtg gccttagcgg
     1321 ataaattcaa agcgaaattt ccgacccggt gtaatggtgt cgatgagaaa ttcttcttca
     1381 ggcagaattt tcgcagctat attagtccgt tcttccggct caagttctcg ttttacaatc
     1441 tgcacttcgc ccatgtagcg cagaaaatcg acattgtcaa tggtgatgtc gccgcggcga
     1501 agactgcgga atccggcact gtccggttcg acgatgcggc cgtctaaaag gcttcggctg
     1561 ttgccggcgc ggacgacgtc gcgtgacggg tcggggcggg acgtaaaggt atgggacagg
     1621 aggtcctgta cataggggtc gcggattaag aaacgggctt ttaggacgac gacacctgtt
     1681 tcttcccggc cggcgcggga cagttcgcga agctccgtaa gggaaggccg gtcgtcgccg
     1741 atgaagaccg actgcagtcc taaggcggcc agatggcggg cggccagact gacgggctga
     1801 cggcgatgtt cttcgagggt cggcagtcct tctgataagg gagctcggcg tccctcgctg
     1861 ctggcaatga aggcacctac ggaaagcccg taggattgga gcaggttcgt ctgggcgatg
     1921 aaataatcgc ggtcaaggcc tgtgtgcggc ctggggtaga agttgtgcag tccgtcgacg
     1981 gcttccaagc gagctccttt tttttgtagc tctttcaagc tgtcttccgt caaggtcgag
     2041 gcattgagtt gtacctggat gacctgactg tagagggcta ttttttcggc atcgaagccg
     2101 tcgtcgaagc gaacgnnnnn nnnnnccatt cctaagaggg ctgccgaggc cggagagatg
     2161 tcggcgacga cgtccatgct attttcgcgg gctgatgcca ggacggcctc cagttctcga
     2221 tgaaaggctg cgacatccgt ttcaggaata tggaaagagg tgaacagacg gtgaatgccc
     2281 aaggctgccg cttcttcaat aagtcccgtt ttttctcgga tgctgcctcg gaggccgggg
     2341 tagatcgaaa tgcctgtgtc catttattca tcctcctttg gatcatcaaa acccagcaga
     2401 tatgtagcta tgaagccggc tgcataggct gccaagaggc ctaagagata catggggatg
     2461 ttgtctgttc cggcagccag gggcaggccg gaaataccaa ttgtggcagc gcctaccata
     2521 taggctgcct ggacggctcc gccgacagca ccgccgatgc aggccccgat gaagggtcgc
     2581 cctaagggga gtgtaacgcc gtagatgagg ggttcgccga tgcccatgag gccgaccggg
     2641 agggccgagg caatggtctt tttcaaccct ttgtttttgg ttttcagata gacggccaga
     2701 gaggctccga cctgaccgcc gccggccatg gccaaaatcg gcagcaggat ggtcacgccg
     2761 tagcgggaca ataattccgc gtgaatcggg gtcagccctt ggtgaatccc gaccatgacc
     2821 atgggcaaaa aggtcccgcc taaaataaag cctgtcaagg ccccgccgct tccgatggac
     2881 tttgtggcgg catagccgat ggcttcggat aaatagccgc ccaagggctg acagatgaag
     2941 agggccatga aggtcgagat gacgacgacg gctaagggcg tgacaaagag gtcgaccata
     3001 tccggaacga tgcgatggag tttcttttca agccatgatg aaaagaagac gaccaagagg
     3061 acggcgatga tgccgccccg gccgggaacg aggttatccc cgaagagcgt gatctgtgcc
     3121 agaccgggat gactgaggac ggcagccatg acgccgccta acatgggaga tccgccgaat
     3181 tccttggcgg cattgatgcc gacgaagata ttcagtccga aaaaggcggc gttgccggca
     3241 atggccagca tttgaaataa aggcccggcg gccagggtcg ggtcgacctt cacggcgata
     3301 ttgagcaggc ctgtaatcag accgcaggca ataaaggccg gaatgatggg gacgaagatg
     3361 tgggcgattt tctttaaggc ctgtttggcc ggcgtcgcat ttttctgtcg gatttcttcg
     3421 tgcagggctt tgccgtcaaa ggggctttgc ggacttcctt ctgcaggctt ttttgccgat
     3481 ggcttgctgt tactgtcatc atcgacgatt tttgcaaaag cctgatagag tttcgttgcc
     3541 tttccggggc cgacgataat ctgccattcg tcgcccgaac ggtttacgcc catgacgccg
     3601 ggaagtttcg ccaggtcttc tttggtgaaa tgctcgtccg ttacggtcag gcgcaggcgg
     3661 gtcatgcagt tgtaggcttt ttggatattt tcggagggac cggtgaggtt ataaatatcc
     3721 ctggccagtt tatcgtaatc catgggaagc ctccctgatt acggcagcga cgtagccctt
     3781 cgatgccgct aatttggcgt ctgcttctgc ggcagatagg ccgaggagta ccatgacgat
     3841 agctgttttg gcatggccgt tactttctgt caaggcgtcg atggcatcct ttcggctgca
     3901 gccggtggcg gtcatgacga tgcgccgggc tcgttcttcg agcttcttgt tggatgattt
     3961 tacgtcgacc atgagattgc cgtatacttt tcctaagcgg atcatggaac ctgtcgacaa
     4021 catattggca atcatttttt gagccgttcc ggccttcatc cgcgtcgagc cggtgacgac
     4081 ttccgggccg acgacggcac agaggtcgat atcggcacag gccccgatgg gggagtttgg
     4141 cgagcagtca atggcaatgg cagcggcgcc gatacttttg gcataggtca gggcgccgac
     4201 gacataaggc gttctgccgg aggcgctgag gccgacgacg acgtcgcggg cggtaagctt
     4261 tttttctctc aaatcggcct gcccgaggtc cgacgaatcc tccgcccctt ctttggctcg
     4321 gaaaatggca tcatagccgc cggcgataag gccttggatc tgttcggggt ctgtgctgta
     4381 ggttggcgga cattcgacgg catcgaggat tcccaatcgg cctgacgtgc ccgatccgac
     4441 atagaaaaga cggccgccct gacgcagccg gtcagaaatg acgtcgacgg cacgggcaat
     4501 ggccgggatg atttccttta tggcttcggc aactttatgg tcttcatcat tgatgacgtg
     4561 cagcatggcc tccgtcgaaa gccggtcgat ctgttgactg tgaggatttc gctgttccgt
     4621 tgccagtgat tctaattcaa tcatagtatt cctttcttac cttctttcct cggtgatgaa
     4681 gagcggtctt ggcggctctt tcaggaaagg tctctatata tctaattata gagagtgttg
     4741 acagtagttg caatagggct ttttttcgga aggcatgcct ttattttttt gctgtttcat
     4801 ggtattatta atctgcatgc agcaggcctg tgtgtcatgt gcgcccatag ctcagctgga
     4861 tagagcagca gtttcctaaa ctgcgtgtcg gatgttcgag tcatcttggg cgcaccattt
     4921 ttttatttgt aaattatata cttactatta atccataata ctattaccgg aaaggtgggt
     4981 ttcctcatgt ctgatatgaa ggaccgagtt gcttctttcc atgaagaagt catgcacgat
     5041 gccgatttta cgctgccgga ggcgattgct gaagccaaac gctgcctcaa ctgcaaagtt
     5101 cctcagtgcc gcaagggctg tccga
//
LOCUS       DAWDXZ010000071         5115 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4436_length_5115_cov_8.702964, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000071 DAWDXZ010000000
VERSION     DAWDXZ010000071.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5115)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4436_length_5115_cov_8.702964"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1149..1158
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2366..2375
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3881..3890
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1186 a         1420 c         1376 g         1103 t
ORIGIN      
        1 cgggccattg aaataagctc tgaatacgac ggcgaagatg tccatatttt aggctacggg
       61 atcaatcccg acgatgaagc catgcaggaa tactgccgcg agttcaagca gcggcgcttt
      121 gaccgggcca tggaaatcgt cgaccgctgc ctgaaaaacg gctatgacat cgaccgcaag
      181 gtcgtcgaag acatggttca taagggcggt accatcggcc gtccccatat tgcccgcatg
      241 ctcgtcgaaa agggctattt ccctgatgta aagacggtct ttgacaaggt tctgtatcat
      301 ggcggccccc tctatgtgcc ctatacgcgg cggaccatcg atgaatgtgt ggccctcatc
      361 cgcaaggtcg ggggactggc cgtcttagcc catccgggcc tcttgaagcg gaccttggat
      421 gaagtcctga cccatgattt cgacggtgtc gaagtctatc atccaaagaa ccgcggccgt
      481 ttcgacgaat ttttggaatt agccaaaaaa cgcaattggt atatcagcgg cggctctgat
      541 tttcacggga ccgtcggccg tttcccagaa aagctcggca tctttaccgt cgatgctgaa
      601 aaggtacagg ctttgttgtc tcacgtaccc taataaaggc aggtgacaga catggaagtt
      661 gtcggtttta tcggcgccag cgggacgggg aagagccacc atgccctggt cgtcgcctac
      721 gattacgata ttcagaccat cattgatgac ggactcctta tcgatcacaa ccgcatcgtt
      781 gccggccggt cggccaagga cgaaacgaat cgcctcaagg ctgtgcgccg cgccgtattc
      841 aataacccgg acgacgccgc tgccatgaag gcagccttag ccaaagcagc tcctgaaaaa
      901 ctgctcatcc tgggaacgtc gcggcacatg attcaccgca tctgcgaagc cctcgagctt
      961 ccggaaccgg cgcggttcat ccgtatcgaa gacgtctcgt gctcggagga aatcgaaaag
     1021 gctcaaagta tccgcatcaa agagggaacg catatcatcc ccgtgccgac gatggagctg
     1081 aaaccccatt ttcgcggcta tctgctggat tcgatccgtt ctttctttta tgacaacaac
     1141 ggcaagaann nnnnnnnntc gcccttcgag cgttccgtcg ttcgtcccat cttcagttat
     1201 tatgggaaac tgacgttttc caacgacgtc cttgaaagcc tggtccgcca tgctttgagt
     1261 caaaatgaag gcgtgaccaa gatcaaccga ctgaaggtgg ctatcaacta taactcgacg
     1321 cgcaacggtc tggctattat cttgtccctg accattgcct acggagaaaa tataaagaaa
     1381 ctcatgagcc gtatccgccg cgatatccag cacgaagtgg aatatacgac gggcatggct
     1441 gtggaaatcc tcaagattac cgtgcgcgac atttcgccgc agcctccgga agaggaatca
     1501 tagagggtac gacctgaaag gggctgctga cggcagctcc tttttcttga cagcctgaat
     1561 cggcggacgg tataataagg tgaagcatgt gactgacgga agtggagtta ccacggggag
     1621 catgattcat aagagccgac cgtctgggcg aaacgttcca gaagtgtcgg tattttttta
     1681 ttttgggtct atcaggaggt atcacgatga acgaactgaa attaaaatac ggctgcaacc
     1741 cgaaccagtc gtcgtcccgg gtctatatga aggatggcag cgcccttcct tttacggtcc
     1801 tttgcggccg tcccggctat attaatttgc tcgacgcctt taacagctat cagttggtca
     1861 aggaattgaa agaagcgaca ggccttccgg cagcggcttc cttcaagcac gtcagcccgg
     1921 ccggcgccgc cgttgccacg gagatgagtg ataccctgaa gaagatatat tgggtcgatg
     1981 atctgccctt gtcgcccttg gctacggcct atgcctgcgc ccgcggtgcc gatcgcatga
     2041 gttcttacgg cgatttcgtt gccctctccg atgtctgcga cgtcgaaacg gcaaccctct
     2101 tgaagcgcga agtatcggac ggcgtcattg cgcccggcta cagcgacgaa gccctggcta
     2161 ttttaaagac taagaaaaag ggcaattact gcgtccttca gatcaatgcc gattacaagc
     2221 cccagcctgt cgaatcgaaa gaagtctacg gcgtcacctt tgaacaggaa cgaaatgaag
     2281 ccaagatcac ggccgatatg ctgcagaaca tcccgacgca gaacaaggac atcccggcgg
     2341 ccgctcagcg cgatctcatc atcgcnnnnn nnnnncaccc agtccaactc cgtctgctac
     2401 gtaaaagacg gcatgaccat cggtgtcggc gctggtcagc agtcccgcgt ccactgcacc
     2461 cgtctggccg gcaataaggc cgacgtatgg tggctccgtc agaacccgaa agtcttgaac
     2521 ctgcccttcc gcgacgacgt ccgtcgtccg aaccgggaca atgccatcga cgtctacatc
     2581 tccgacgatt acgaagacgt cttggctgac ggtatctggc aggatctctt tacggaaaaa
     2641 ccgcagcccc ttacgcggga agaaaagaaa gcctggattg cccagctcca cggcgtagcc
     2701 cttggcagtg atgctttctt cccctttggc gataacatcg aacgggctca tcgcagcggc
     2761 gtcgattaca tcgcccaggc cggtggttca atccgcgacg acaatgtcat cgaaacgtgc
     2821 gataaatatg gaattgccat ggctatgacc ggcctgcgcc tcttccatca ttaaacaaaa
     2881 tacccttgcg gcaactgtaa gataggtttt cgcagatttg ggcccatggt gagacttccc
     2941 gtcgtcatcg tgggcccttg ctgtatgtac cattatgctc gatgatgaag aaggggggat
     3001 cgcatttttt tagataattt tgccaaggtt cctctgcggc gatataatga acatatatta
     3061 tatagataaa taaggaggga attctatgac acaatatagg tcggtctgcc cctatgactg
     3121 tcccgacgcc tgcggcctct tggtcgacgt tgaagacggt cgcgtcgtct cggtgaaggg
     3181 cgatgaaagc catcccttta cgcgggggac gatatgcccg aagatggctc actatgaacg
     3241 gacggtctat tcgccgcggc ggctgacgac gccgctcaag cggacgggac cgaaggggag
     3301 cgggaaattt gagcccatct cgtgggatca ggccctagct gaaatcgcag cttcgtggaa
     3361 aaaaattatt gccgaagagg gaccagaagc catcttgccg tactcctacg ccggcaccat
     3421 gggcatcgtc cagcacgatg ctctgcatgg cttgttttat cggctaggtg cgtcggaact
     3481 cgaccggacg atttgcgcac cggccaaggg gcaggggttc aaagacatca tgggcggtac
     3541 cttgccgacg gcgccccagg aagcgcaaaa cagcgacgcc atcttcttgt ggagcattca
     3601 catgatggcc accaatatcc acttcaagca cgacgtcgac gctgctcgtg aaaaaggggc
     3661 tgctatttgg tgcatcgata cgtatcggac caagacggcc gatttggccg atcacttcat
     3721 cgccattcgg cccggtaccg atggggcact ggctctcggc atgatgaacg tcctggtccg
     3781 cgacggtttg accgacgaag cctttctgtc tgaaaaagtc cgcggttggg atgaattgaa
     3841 agaaaaagta atccctcgtt atacgccgga agaaacggaa nnnnnnnnnn ccctgacgga
     3901 agacgacagc atccagagcc tcttcgtcta ttcgtcgaac ccggcctgta cggcgccgga
     3961 tcaaaataaa gtcctcgccg gcctttctcg agacgatctg tttaccgtcg tccacgaacg
     4021 cttcatgacc gatacggccc gctacgccga tatcgtcctg ccggcgacga cgtccctgga
     4081 gcacgacgac gcctattatt cctacggcaa ttacacgatc cagtgcagct atgccgtcat
     4141 cgaacctctc ggcgaaagcc ggtccaactg gcgtcttgcc tgtgatctgg ccaaggctct
     4201 cggcattacc gatccctttt acaatcagtc ggaacgggac cttgtcgata aactcgtggc
     4261 ctcgacggtg aaatggcccc ttcccgtcga tcaggactgc ctgcgctcag gccggccgac
     4321 acctcttccc atgccggaag actatcagac gacttttgcg acgccgtcgg gaaagatcga
     4381 aattcgaaac gatgccctga caccggccct tcccgattat atacccctcg atcccgatga
     4441 gggaaaactg gccttcgtca acggcgccga tatgcgcatc ctcgactcgt cctttaatga
     4501 acgggatgaa ctgacggcga gccatacgat ggacctcttc gtccaccctg aggatgccga
     4561 ccgctacggc attgctgacg gtgattccgt gaccctcagc aataaacggg ggcaggccga
     4621 ttttacggtc cgcatcagtg accgcaccgt accgggccag gtcgtcagtg aaggtgtatg
     4681 gtggcgcgaa cgggtcaaag acggcggccc gtcggtcaat gtccttacca gccagcgttt
     4741 gaccgatgaa ggccggggca gtacttttta cgacgtccgc gtcgatctga caagtaaaaa
     4801 tacttgacag gtcatccctt ttcgggtata ctgtcacagt acgctgtttg aaaggcggtg
     4861 aagccatgct tgaagacgta gtccgtaagg gacggctcct cgatctgtac gggccgttat
     4921 tgacggagaa gcagcggcgc tgtatggaaa tgtatttcga tatggacctg tccttgagtg
     4981 aaatcgggga agaactgcag atttcccgtc aaggcgctta cgacatgctc aagcgcgctt
     5041 cccattcgct ggaaagctat gaacagcggc ttcagttgct ggcccgcttc gatgcggtcc
     5101 gggaacagat cgatg
//
LOCUS       DAWDXZ010000072         5058 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4470_length_5058_cov_9.313812, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000072 DAWDXZ010000000
VERSION     DAWDXZ010000072.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4470_length_5058_cov_9.313812"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2684..2693
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3942..3951
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1090 a         1379 c         1416 g         1153 t
ORIGIN      
        1 cagctcttcc cccgtgtcgt cgaggtagag atggggatac atatcgctga tatgcatttc
       61 catacggatg cgcaggtctt ggcgatgttc atcgggttta tcggcaaagt actccttgtc
      121 gctctcgagg agataaagcc ccatggtgat gatgatgggc tgtgacggat cggcataatc
      181 gtcttcgtcg acgttgcggc cgtcgctcat atatgccaaa aggcgcagga gaccgctctt
      241 gccgatggca ttttccccga cgatatagtt tgccttttca tgaaaggtca tgtcgacatc
      301 ttgtaagttt cgataatttt gaatgcgcat gtgtgtgata tacacaacga ttcctccttt
      361 ttccggatat ccttactata ggtattcgac aaaaaacgcg ctgctccctt cttttgagag
      421 acagcgcgtt attccatttc ggcaaggaga cttagcggcc gcttttacgg caaagtttcg
      481 ccaatcggcg agccaaatca aaggtcgtct ggcctccctt catctgccac tgccagttgc
      541 cgtcggcgac gccgggaacg ttcatccgag cttctgccgg gagggccaac agatcctgca
      601 tgggcacgat gaccgtttca gcccggcggc ggtaggtgta ggcgatgaca gcctgtacca
      661 gctcttccgg cgagacgtcg tcagaaaggt ccagcatgcg gcgaatatga agctgttcgc
      721 tttcagaaag ttcctgttca taccatcccc gaatcgtatt gttgtcgtgg gtccccgtat
      781 aggcgatgca gttcggttct gtatcgaagg cgcgaatgcc gtcggaacgg tctttaagat
      841 ggaactggaa taccttcata cccgggagat caagggcgtc ttttaaggcg cagacgtcat
      901 cggtaatgac gcctaaatct tcggcaacca ggttcagtcg gcccagtttt tgatagacgg
      961 cgcggaacaa atcgaggccc ggcccttctt tccaggcacc gtctttcgcc gtcagcgctc
     1021 cctgagggac ggcccagtag gcggcaaagc ctcggaagtg atcgacccgc agttcatcga
     1081 agaggtcttt catgcggcgg aaacgatcgg tccaccactg atagccgtcg gcggccatgg
     1141 cttcgtaatc atacaaggga ttgccccaaa gctggccgtc ggcactgaag tagtcaggcg
     1201 gtacgccggc gaccgatacg ggccgcccgt cttcatcgag gtcgaactga tcctgatgag
     1261 cccagcagtc ggcactgtca tgggcgacga acatgggcag gtcgccgagg atggtgatgc
     1321 cgttttcttt ggcatacgcc cgaagctcgt gccactgacg gtcgaagatg tactgcatga
     1381 atttgacatg ggatacggat cctgccaatt cctttgcata ccgcgtaagg gctgcggcat
     1441 cgtggcggcg gatgtcatcg ggccattccg tccaaggacg ccccttaaag aaatcacgga
     1501 tactgcggaa caaggcgtag tcgtcgagcc agtaggcatt ggcctcacag aagtcccgga
     1561 agggctgcca ggaaatgatg aggtccttat cgtgagacag attccataag atgcgggcct
     1621 tttcggccgc cacctccgat ggcggcaagc ctttccccat agcgatgaaa tcggcgacga
     1681 cgtctttttt cagccagccc cagtcacaca gtcgttctaa agaaatgagg gattcgtttc
     1741 cggcaaaggc cgattccgag agatacggcg aatcgccggc caagggcggc atcagaggca
     1801 ggatctgcca gaccgattga ccggccgtct taaggaagtc gacgaatcga tacgccgccg
     1861 tccctaagac gccctttccg acagctgccg gcagtgacgt cggatggaga aggacgccgc
     1921 tgcgtttctt ttcggcctca ccggcctgta agatgaccgc ttctaagggc ggcagggtca
     1981 aggtcaggcg gctgttcaag gtcacttccg gttccattcg cttattcagg gcattggtaa
     2041 acttgccgta agccaatccg tcggtataca ggctgatggt ccgaagcgcc gacggattcc
     2101 ggttcatggc gacgatgaac agcccgtctt ctgccggttt cccaaagacg tctcggccgc
     2161 cttcgatgag cctggcaaag gcgtaaacgt cgccgtcggc gtagagcggg atgaaacggc
     2221 ccgtctgtaa ggcatcatga gccgtccgca aacgcgtcaa agccttggtc caaccgagaa
     2281 tatcccagtc ttcccgtccc caggggtagg tccgacggtt gtccggatct ttaccgccgg
     2341 tgagaccggc ttcatcaccg taatagatac agggcgcacc gggcatggtc atctgccacg
     2401 tcgtcaagag tttcaacctc tttttggccg tcgtttcgac atcttctacg tcggtgccgc
     2461 cggcggcact caggacggtc aagattcgtt cgatgtcatg gctgctgatg aggttgagca
     2521 tggcatagaa attttccggc ggataatttt cccgaaggcg ggtcaattca tcattcatgc
     2581 gccgtgcctc tttggctccg gtgacaaaat ccaccatcaa ggccctttgg gcgtagttca
     2641 tggcgctgtc gatgtcgtag cccgagaggt attcccgctg ctcnnnnnnn nnngctgctc
     2701 gctgtagctg accttattcg acgcgtcttc ccagacttcg ccgatgagga cggcgtcggg
     2761 attttcttct ttcaaggtct tatagaactg ccgcaagaaa ggaacgggca gctcgtcgat
     2821 gacatcgagg cgccagccgc ggatgccctg attgagccag tatttgagga cgctttcttt
     2881 cttgttaatg atgaaatcca tgtacgacgg cgtcgtttcc tcgacatcgg gcagatccga
     2941 cacgccccac cacgattcgt attcatccgg atagttggtg aatcggaacc attcgtagta
     3001 cggcgattcc ttcgattggt aagcgccgac gccgggataa tgaccgaagc ggttgaagta
     3061 gatgctgtcg gccccggtat ggctgaaaac gccgtcgagg atgacggcca tgccggcctc
     3121 tttggcagct ttacagaagg cggcaaagtc ttcattggtc cccagcatgg gatcgacgcg
     3181 gaaataatcg gccgtgccgt agcggtgatt actcgagctt tcaaagatgg gattgaggta
     3241 aatggccgta atacccaatt cttttaaata aagcagtttc tcctgaatgc ccttgatatt
     3301 gccgccaaaa aagtcataat aaacgatatc gccgacgcct tcccgcttgc agtagtaagg
     3361 cttatcatac caattactgt gaataacggc cccttctttg ccccaaaggt tttctccgtc
     3421 gccgctgcga tagaagcggt cggggaaaat ctgatagacg atggcatgct taaaccagtc
     3481 cggcgtatgg gcctctttgt cgtagaccgt aatctgatag gccggcggcg ccgcttcata
     3541 gacggcccct tccccgccga gctgttcgta attattcccg taataacgcg tcgtgtctcc
     3601 gtcgcttacg atgaagtaat accataagag gcagccctta tcgggaatgg gcacgacggc
     3661 tttaaaggcc tggtcctcat aagtcatgga gagatgcgat tcaccgacgt tactctgcca
     3721 caggcgcacc tcggcccggc aattctcccc ctgtcccgaa gcccccaggc gcaggataag
     3781 ccggcttcct gacgggacgg caccgaaggc agaccgataa taaaaatcat gagagttgtg
     3841 atacagtcgt aagtccatag aaaatccttt cttcttcatc aagaatcccc cgtcgcgcat
     3901 acccgacggg ggacccgata ctgagcccct tacagctgca gnnnnnnnnn ncgggaaggt
     3961 caggccgttg ccctgtttgc tgtacttatc gaagggctgt accgtatcct tgagtccgcc
     4021 cgtttcccgg acgataggaa tggatccgta gcgaagggcg atgagctggc tgagaccgca
     4081 cggttcatag cgcgacggca tgaggaacat atccgacgca gcgtagacct tatgagccag
     4141 ggcttcattg aacatgatat tggccgagac cttgttggga tggcggtaag ccagttcccg
     4201 gaaggcatct tcatagccgg catcgccggt cccgaccagg accagctgga cttcgtcatc
     4261 gacgagttca tccatgatcc gcatgacaag gtccaggccc ttgtcttcga cgaggcgcgt
     4321 gaccatagcg atgaggggta cgccgcgttt gatacgaagg ccgagttcag cgcgcagggc
     4381 gtctttattt tcccgtttgt ccgcccgggc cgtatcgaca gagtactgct ttttaatgta
     4441 cggatccgtt tccggattgt aagcatcgac gtcgagacca ttcaaaatgc ccgacacttt
     4501 gccgctgtta gcccggatat agccgtccag gccttccccg aagtagggat aggtgatttc
     4561 ctgagcgtac gtcgggctga ccgtcgtaac ccggtcggca tagaccaggc cggacttcat
     4621 gtagttgacg cagccgtcgc attcgaggtt gcccttatca aagagttcct gcggcaggcc
     4681 gatgatgtcg cgcataatcg acggcggata gatgccctga tacttcaggt tatggatggt
     4741 atagaccagc ttcatatcct tataaaagtc gtcgtggcgg aagtcttctt tgaggaagac
     4801 gcccatgagc cctgtatgcc agtcgttgca gtggatgata tcgggcttaa agccgacggc
     4861 gggcagcatg gtcagaacgg cacggcagaa ataggcaaac cgttcggcgt catcgccgta
     4921 gccatagaga ttgtcccgct tgaaatacca ttcgttgtcg acaaaataga cggtcaaccc
     4981 gtcgtgttct accgcttcga cgccgtaata gagctggcgc caagacatgt tgagcggacc
     5041 ggtcacgacg ggcttcat
//
LOCUS       DAWDXZ010000073         5057 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4474_length_5057_cov_8.438625, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000073 DAWDXZ010000000
VERSION     DAWDXZ010000073.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4474_length_5057_cov_8.438625"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1264..1273
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2413..2422
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4120..4129
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1498 a         1046 c         1246 g         1237 t
ORIGIN      
        1 gacggtaggg ggattttttg tacagatata ggtctaattt tctgtctaaa aattattgat
       61 tattttttag acaaatgcta tactacgcat aacgtatcga gaacgtgcgt ccgtattaga
      121 aaggagtgcc tacgatggcc gaagataagg gaagggaatt ggaacaacgg cagctctctt
      181 tgttcgaacc tgaagacggg cagacgaaaa gccccaatct gctgcgctgc tataagaatc
      241 gtctggtcag tattgaacac atgtcggtcc acgatttatt caacggcttc gatgaaatcc
      301 gggtcatcac tttttcttac gatgtcgcca tggttcattg gcttatgaag aagtttaaat
      361 atggaaaaat tcttctcgga gccgatttca tggtccggaa agatacccga acggcagaga
      421 aagtagccgc cattctggca ggggcagacg tggcagcaca gaaaatccgg aaatacagtg
      481 acttagttca aaaaatgtta gacggcaatt tggtcattca cagctccatc aacgtcttgg
      541 accaccgaaa attatacctc cttcgcagtg acgacggacg gactcgggtc atcacgacgt
      601 cggccaatat gacccgaagg gcatggtctg ccgaccaaat ggagaacgtc gccttttatg
      661 atgaaccgaa tgcttacaag gcttaccttc aggaattctt cacggcctgg aagatgtccg
      721 ccgagattcc ttataaggtg gtcgccgctg aagaaacgga cagtcctaaa gaggccaacg
      781 ccgtcttaaa gaaagtggtg gaaaccaata aagccgtcat cctgcaaacg ccggcagaag
      841 atgaacagac ggtcatcacc aactatcagt atgtcctgga tggcgataag gccagagaaa
      901 aatacctcga actgttgaag gatacgggga tccgaacgac aaaagacggg gtgattaagc
      961 tcaatcccaa aatcgtcgaa aaaatagagg tcaatgcgaa aaaagcaggg atacgcaaaa
     1021 tcaaactcaa gcaggtcgtc gagccatatc ctgaaatcac ctttgattat caaaatttgt
     1081 ccatgaatct ttccggggaa ccgatgaatc tgaatccgcc ggaagaggaa gttaaagccg
     1141 acatcaagta tctgttagcg atttttgaga aatacaactc cttcatcgga acggatccgg
     1201 aaaagcagca agaaatctac tataaactgc tcaatgtcat gtttgcttcg cccttttttg
     1261 cccnnnnnnn nnnatttcaa gataaaatta tcattttgtt aaaaataaaa gccatcttga
     1321 aattgatgac gggaaaggaa gatttaagag tcctggcagc taaaacctgt tcgagtaaaa
     1381 cggccatcgc cttgcaggta gaagggaagg gcgtccccct ttttatcgat gaaatcaata
     1441 atgcctactt gtcacacatg aagaccacca tcacggcaac caatcacttc tgcgaaaacc
     1501 tgcagcttga cacggtgccg atggttattt tcgcttcgaa tgatgtgatc gacccggcga
     1561 tggaattccg taaacgaatg atttttctca ccccgaaagg gagtattcct tctgatgtcg
     1621 atcaaacggc ctggctttct gccggcaaaa gtttgattgc cagactggga acggggctgt
     1681 atggtgaata tatccgacgc atgctttcca aggtaaggga tttaatcgat caaatgcaaa
     1741 cgaagggagg tgctggtttt cctaagggct ggtatccgga catcatgccc ctttcttcgg
     1801 aaaccttgat tgaaatcgtg aaggacttcg gtttcgataa gcccgattac ttcagaatgc
     1861 tcaattggag cgatgatttt tctgaaaacg cccgtcatat tgcagccgac gctttccagg
     1921 aaatcagaga gatgtacact tctaacaaga gagtattcac aattgacaaa aacacagtta
     1981 caatagaaac aggttcggat aaagagcgtg tgcgccagat taaatcttgg gcagccgtac
     2041 tgcccagaga agtcatcaaa gaagagccca tgtggataaa aacgggctgg atgatcatct
     2101 ttgatcgagg cgaattggaa aaaaggatgg gcatccgatt taaggacccg tggtaccgga
     2161 aatttttttg aaaatgaatc gaggtgaggg tcatgcggcg gctgtattta tacaaaaaat
     2221 tcactgaaga cggcgtggct gtccgggatg cggcagctga tatcgttcgg cctgatgatg
     2281 aagtattcgt cgtaacggaa catgatcgta agcacgaaga actcgataag gtcatcgaaa
     2341 ggggccaggc tcaggatatc ctctttatcg aaggcatgga tgatttggga caaatggcag
     2401 aagtcacgat cannnnnnnn nntcaaaagg ctgcggaaca tcatggaaaa gggcatctgt
     2461 ctcgtttctt gtgatttgaa aataacctat gaacacgggt tggaactgaa ccaaaccatc
     2521 gtcgggacgg tccttgaaat gcttgaaaaa acactgaaaa cggaccatcc cttggtactt
     2581 cgtccgcgaa atgccggacg gcccagcatc gactttcccg acggttggga tgagaaatac
     2641 gacgaatgga agggcgggaa actttcttcc aaagccttta tggaatggtc gggaatgaaa
     2701 aaagcgacct tctataataa ggtgacagaa tatagcgaac tattagagga agaagaaaga
     2761 taccgtaagg ctattaaaag ggccggctgg aatcgataac ctctttatga tagggagcag
     2821 gaatttctga aggatttgtc gaaattatta ggtatgtgag gtgatactta tgagtgaatt
     2881 gacctttgta tgctattctc gttgttctac ctgcaagaaa gcggaaaaat ggctggaagc
     2941 caacggcgtg tccgtgacgg tgcgcgacat caagggtgat aacccgacgg ccgaagagct
     3001 tcgtgagtgg cacaaaaaga gtggattgcc cctcaaacgc ttcttcaata ccagcggtct
     3061 caagtatcgg gaattgggac tcaaggacaa actggcagac atgagcgaag acgaacagta
     3121 cgaacttctg gcgacagacg gcatgctggt caagcggccc ttactggtcg gtaaaaacac
     3181 ggttctcgta ggattcaaag aagccgaatg gcaggataat ttgtaatata aaatgagcct
     3241 ccgtctgcga ggctcgtttt cgcatgcctt caaatcgttt ggccgttggc ccggactgga
     3301 gcgaaggaag gacgattact gaaggcgttg caggagcgct gattgaaata aggtactgtc
     3361 ggggaattgg cgaagagagc ggaatggaca ccgcctgtga aatccaggcg atgatgcgca
     3421 ttccatagac agaccgaaaa aagtcgtgaa gtgagatttt ccttgactcg ataccggact
     3481 ttgatagccg ggcaaggagt cttagtatga tgtttcggat agtgaaggat ttttacgagg
     3541 tgataacatg ttaggcgcaa tcgtaggaga tattgtcggc tcacggtttg aatggcacaa
     3601 tatcaagacc aaagaatttg aattattcca ctataaatgc aggcctaccg atgacagtat
     3661 catgagcctg gccttggcta aagccatctt agacagcaag gccgattaca gtaatctcgg
     3721 tgaacttgcc gtggacaata tgcagagcct gggccggaaa tatccgaact gcggctacgg
     3781 cggccgcttt aaacactggg tattcgccgt cgatcccaaa ccctacggca gcctgggaaa
     3841 cgggtcggcc atgcgtgtca gtcccgccgg ctttgcggcg aaaagcttgg aggaagccaa
     3901 attactgtcc cgaaaaatca ccgaagtcac ccatgaccat cccgaaggat tgaaaggggc
     3961 cgaagccgtc gtcgtggcaa tctatatggc cagaaccggc tccatcttac aagaaatccg
     4021 agacgtcatc gatcaagatt actatcccat ggatttcacc ctcgatgcca ttcgcccgac
     4081 ctacgaattt gacgtcacct gccaagggag cgtcccccan nnnnnnnnng atggcttttt
     4141 ttgaatcgac gagttttgaa gatgccatcc gcaacgccat ctccatcggc ggcgacagcg
     4201 acaccatagc cgccatctgc ggcagcatag ccgaagccta ctacggcatt cccgaaaaac
     4261 tgcggaaaca gaccctgatg tttttagatg aaagactgtt gaaaatcctg ctcgattttg
     4321 aaaaggtcta tccgccgatt gtggaaaaac gggtgtaagc gtttctgcag cgcaatgaat
     4381 agtgaaacta agctcccttg ttgaaaagcg aaagcaacca aacaatcgaa ctcaaatcat
     4441 tttattcttg aaattataag ctctgcaact cgtatgaata cgcggcttgc agagcttttt
     4501 catttttgga cagctgtggc ttagttataa tgagggagaa ggtgaaaacg tgatttctat
     4561 tcatcatgcg aaagacatac ttgtaatcag tggattggtg gcattctttt tatatgctta
     4621 aaaaaaagaa ttgcagaaaa actttgataa aagcatgttg cgtgtttatc ggtatagcct
     4681 tccttttttt gtaggaattt ttataacttt tatttcagtg ttagaatttg tgtattcgtt
     4741 ttactatgca gtaaaatatg ccccctggtt atttatatca gcttgcatgg gatatacgct
     4801 tgtgatttat agtgatggca ttttagcaaa ataccaaaat aatgaaagaa gatataaaga
     4861 aataatggat atagcaaaga attcggcaaa atggctaatc atcgctttat ttgcacttat
     4921 tatgaaagag tggtttattt aaataaagcc ttgacccatg gtatcgtagc agaaatctcg
     4981 tcttatattg ctggaatgga gaaggcttat aatttttgga gaacctcgtc ttattgtcat
     5041 gagggagaag gggaaaa
//
LOCUS       DAWDXZ010000074         5053 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4476_length_5053_cov_9.906963, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000074 DAWDXZ010000000
VERSION     DAWDXZ010000074.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4476_length_5053_cov_9.906963"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    510..519
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4407..4416
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1313 a         1284 c         1280 g         1156 t
ORIGIN      
        1 tttgttgttg taattataca gccattggaa tcagtcgtca atagattatt aagtatattt
       61 ccatgtttta tcattaagtt aagttttaca aaatgtaatg ccgtttatta taacgtttaa
      121 cttaaggtaa ataattattt atgccaaaat atccgccact ttttgctgta aaaccggcac
      181 gacatcggct ttaaaccagg gattagatgc ctgccagcgg aaattcagcg ggctgggatg
      241 gacgagtggg aaataatcgg gcaaataggc ttcgaagttt cttaccgtct cggtcaggtt
      301 acgccccttc cttcctttta aatagtaatc catagcgtat ttcccaatca gtaaggtcag
      361 ggacaggtcg ggcatgagag ataaaatatc aggatgatat tcagcggcaa tgaatttgcg
      421 cggcggctta tcgcctgttt tcgccttgcc ggggtagtaa aagtccatgg gcatgatgga
      481 aacggctgtt gagtagaaaa cagaggagtn nnnnnnnnnc gttcatcttg gcgaagttta
      541 tcaatgatgt cctgcaaatt cataacatat tccctccatt cgataaaaag gcagaaacct
      601 gattccagat ctctgcccac aaaagattat atcaaaaagc tgtagtatat gatgggtatg
      661 aataaggccg gcaaaaagtc agcgacctta aatttcgtca gttgaagcag attgagggca
      721 atggccaaaa tcatgaggca gccgacggca ttgagttcgc ggacgacatc gggtgtcatc
      781 agcggcagca agaaggtcgc taatacatag aatatcgttt cataaacaaa aagtacaatc
      841 cccacgggga cgacgccaag tccgtataag gcgccgaaaa taaaagacga tacggcgtcg
      901 atagcgcttt taaaatagag taagtcgttg ctccccaggg cggcctgaat gggtccgata
      961 atagccatcg caccgatggt ctccatcatg gacaaggtca caaacccttt gacgaattta
     1021 gaatcgtcgc cgcggctcaa atgcttttta aggaattccc cgaattgatt catccggtct
     1081 tcaattcgga tggccgtccc gaggaccgta ccgataatca tactggccaa gacgaggagc
     1141 atattctgac tttggacggc cccttgaatc cccatagccg ccagtcccag ggcgatgacc
     1201 cacagaacgg gagtcgtaaa tcgctcagat atccgatttt ttaatccgac cccaataact
     1261 gccgccaggg atgaggtcac tgcgttgaca ataacaccga tcataaactt gcctcctgac
     1321 ttttagacta cgttcattat agcaaaaaaa ggtgctgccg aacatcaagc aacacctttt
     1381 tataaaacaa aatacgttta cgccattact tatgagccgg cgacagcgaa gcacctgtcg
     1441 tctgcctgtc atctgcttca taattatcat caaaacgttt attcaggtag ttgacataga
     1501 gatacgcaag gcctgcccct gaaatattgt gccaagcgct gaaaatcgcg ccggggaccc
     1561 ctgccatggg aagggcggca aaatgggttt tggcaagccc cgtggccaag cccgaattct
     1621 gcatgccgac ttcgatggat acagcaacca tctttttcca gctcatgccg cagaggtgac
     1681 cgataaagaa tcccagggca tagccgaggc agttatgcaa aatgacgact aacaggataa
     1741 ggcccgagct gttgaggatg gaatcgcggc tggcaccgat gataccggcg atgaggaagg
     1801 aaatagccat ggctgacacg gccggaagat agtcgagagc agctccggca agcttcggca
     1861 gaaagcgctt gactaaaagc cccaaggcga taggaacgat gacgatttgt acgatggata
     1921 agaacatgct gacaggatta aaggaaatac gctgaccgat gatcagatac gtcaagaggg
     1981 gcgtcaagac cggagacaat aaggtcgaaa tactggtaat ggtgaccgat aagggaacgt
     2041 cgcctttact catgaaggtg ataacattgg acgacgtacc gcccgggcag gtcccgacga
     2101 ggacgacgcc tgccgtcaga gccggatcaa ggcgaaatac ggtcgccagg acgaaggcca
     2161 ggaacggcat gacgaaatac tgggcacaaa cgccgaaaaa tacgtcctta ggctgcttca
     2221 aaacgaggac gaaatcgctg gcgctcatgg tcatgcccat gccaaacatg atgatgccca
     2281 agagaaccga caagacggaa atgccgccga catggcctaa aacccaagaa aagctcgtcg
     2341 gcatcgtaag gccgatgatg agaaagatga tggcgatgac gccaaaaaac ttaccgatga
     2401 gagcacaaac ctgtttcata ataagcccct tttcatatta ttttatattc agataaaata
     2461 aatctcctgc cccattccct gcgcagcata agcgaagggc acggaagcag gagatagcat
     2521 tcagtcttac tgcatgacag cgccggtatt ggccgacgta acaagcttag cataccgtga
     2581 caagtagccc gtcttgacct tcggttccgg ctgcttccaa gcagctcggc gtttagcgag
     2641 ttcttcatcg ctgacttcca gttccagttt acgattcgga atgtcgatag aaatcgtatc
     2701 gccgtcgtga actaaggcca agggaccgcc ttccatcgct tccggtgaaa tgtggccgat
     2761 gcaggcaccg cggctggcac cgctgaagcg gccgtccgtc aagagggcga ctttcaagcc
     2821 catgccggtg atgactgccg tcggattgag catttctcgc atgcccggcc cgcctttcgg
     2881 cccttcatag cggatgacga cgacgtgccc ttctttgatg tcgccgcgga tgatagcttc
     2941 gatagcatct tcttcagcat cgtagcactt ggccgttccc ttatagacca agaggtcttc
     3001 atcgacagca ctttctttga taaccgatcc taggggtgct aaattccctt taaggacagc
     3061 aatccccccc ttattcatat aggcattgtc gacggagtgg atgacatccc gattgaggac
     3121 ttccgcatct ttaatgcggt cgcctacggt gccggtaatg gtcttagcgt cggtgtgaat
     3181 caagcccaac ttcgagactt ccttcatgac ggcgcagata ccgccggctt catcgaggtc
     3241 ctgcatatga taggttccgc cggggctcat cttgcagata taggcagcct tgcggctgat
     3301 ggcatcgaac tcgtcgaggg acagctgtac cccagcttca tgggcaatgg ccggcaggtg
     3361 cagggccgtg ttggtagaac cgccaatagc catgtcgacg gcaatggcgt tttcaaaagc
     3421 ttcgagggtc atgatatccc gaggcttcac atcgcggcgg acgaggtcca agacgacttc
     3481 accggctttt ttggccagca tgcggcgaag gcccgtataa gcagcgggga tcgtcccgtt
     3541 gccgggaagg cccatgccca agacttcagt catgcagttc atggtattgg ccgtaaataa
     3601 gccggagcag gaaccgcagc ccgggcaggc gtgaaattcc atttcatgca tttcttcagc
     3661 cgtcatctgg ccggattcga ccttgccggc ggcttcaaac atcgtgctga cactgatatt
     3721 gcggccctta tagcgaccgg ccagcatggg accgccactg acgacgacag tcgggatgtt
     3781 gagtctgccc gcagccatga gcatggccgg cacgatttta tcgcagttcg gaatgaggac
     3841 caagccgtcg aaagcatgag ccgtcgctac ggcttcgatg gagtcgcaga tcaactcgcg
     3901 gctcgaaagg gaaaaactca tgccccgatg acccatggcg atgccgtcac agacgccgat
     3961 agacggaaat tcgataggcg taccgccggc agcggctacc ccaagtttaa cggcctgggc
     4021 aatttcgcct aagtggacgt gccccggaat aatttcatta aaggcattac agatgccgat
     4081 caaaggcttc ttcaaatcct catcactata gcccatggca tggaacaaac tccgatgggc
     4141 cgttcgggta accccttttt ttacaacatc actgcgcata atatccatcc tctctttata
     4201 ccgtactggc agcagcatat ctattacata tccttcacgg ccgtttcccc tcgagagaga
     4261 gaaatctggc ccgtccgggc aatttcaatg atttcatagt ctttcagcat ttcaagcaag
     4321 gcttcaatac gatcttcccg gcctgtaatg cgcatggtca gcgaatgtcg ctggacatcg
     4381 acaatcttag ccttgaacaa attcgcnnnn nnnnnnattc ttcccggatt tcgggcgtca
     4441 gactccgtat cttgatcatg gccagttcat aactgacaaa gggggcgtca tcaaggttta
     4501 ccaccttgat gacgtcgatg agttttgaca gttgattaac ggcttggtcg agttcccatc
     4561 ggttttcgac ggacacgacg atattgatgc gggtcacatc ggattcctcc gtgtacccgg
     4621 cgttaataca ttcaatatta atggctctgc ggctgatgag gccggcgacg tgtgtcagta
     4681 cgccgggctt attgtcgcaa agtagtgcca ggtgctgttt cgtcactggc cttcacctcc
     4741 caataccatt tgatccaatc gttttccgcc gggaaccatg ggatacacat tttcatcagg
     4801 cggaatgaat acatcgagca gggccggtcc ctccgtttgg aataaccagg atacggcttc
     4861 atccagtttt tccggattgc ggacacagga gccgggaaca cccatggctg ctgaaagggc
     4921 cgtcaaatcc ggattgtcgg caatgaccga ctgcgaacag tgaccactgt aaaacatgcg
     4981 ctgccactga ttgaccatgc ccaagacgtt gttgtggaga acgacgactt tgacgtcgac
     5041 accgtactta tgg
//
LOCUS       DAWDXZ010000075         5040 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4488_length_5040_cov_8.549248, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000075 DAWDXZ010000000
VERSION     DAWDXZ010000075.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4488_length_5040_cov_8.549248"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    294..303
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4324..4333
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1371 a         1166 c         1309 g         1174 t
ORIGIN      
        1 ttatgaagta aaccattgcc agaaaaaaat ccgcagctgg attgagacgt taggagtgaa
       61 tctataagat gaacgatacg aaagtaaaag taacgattaa tcacgaaaaa atcctttggg
      121 cccattggaa ggacaatgcc acagccaggg ctttgttgaa aaagatgccc tttaccatta
      181 agatgtccaa cctctacgga agggaaatgt gccaccgctt cggtggcggc agcctgcctg
      241 taaaagcgtc gcagaataag gattacgccg taggcgacat ttctttctgg ccgnnnnnnn
      301 nnnacgtcga cggcgatatc cgtttctttg ccggtatggg ggaagccgat gttttatttg
      361 aagtcctaaa ggagggataa tcatgaaatt gtcgaagaaa atcacgacca ttttgatgat
      421 agggttggtg gcagccttgg tggccggctg cggggaagcg gcacaaaatg catctcagcc
      481 gcagcaaaat accagtgccg ctcaggcttc cggtgtcgat gaaagttcat cggccagcca
      541 aaaacgggcc attgaagata aggatatcgt cggcgtatgg atcaatcata tcgacaccga
      601 catcaaccaa aagattactt ttaatgccga tcacacgtgg acggaaaacc agcataatgt
      661 agacaacatc tattccggca cctggtccat cagcggcgac cacagtattt acctcgaacc
      721 ctacggagaa acgattgaaa tcaatcctga taatttgaaa gaaatgaacg tcgtccgcta
      781 ccatcacagg ctgacgaaag aagattcgta aggagaaagg agaattacta tgtctattcg
      841 tgataaagtc atcgtcatta ccggtgcatc ttcaggcatc ggcgcagcga cagccataat
      901 gctggccaaa caaggggcca aggtcatgct tggcgcccgg cgcacgcagc ggctcgaagc
      961 ccttgtccgc gatatcgagc atttaggcgg gaaagccgcg tattatcagg tcgatgtgac
     1021 tcaatttgac caagtccaag ggctggtaca ggccgcaatg gatgaattcg gccgggtcga
     1081 tgtcttattc aataatgccg gcatcatgcc caattcgcct atgagtgacg tccgcctcga
     1141 tgaatggaat gccatgattg acgtcaacct caaaggcgtc ctccacggta tcgctgcggt
     1201 actgcctatt tttaaggctc aaaagagggg ccatattatc actacctcat ccgtagccgg
     1261 tctcaagaac tacgtcggtt ccggcgtcta cggggctacg aaatttgccg tccgcaacgc
     1321 catggaagtc gtccgcatgg agtcggccca ggagggtacc cgtattcgca cgacgaccct
     1381 gtttcctgcg gccattgata cggagcttgt gcaacatatc ggagaaccgg cgacagcaga
     1441 gcggatgaag agtttttacc aagtccacag catttctcct gacgccgtcg cttcctgcgt
     1501 ccggtttgct atcgaacagc cggaagacgt cgacatcagt gaaatgacca tctatccgac
     1561 gagccaggct tgacgagtac gctttctgag attttttata atcgtaaggg caggagaaag
     1621 agtgctgatc cttcttttta ggagggcggc actctttttt tttagcattc aaacaatggc
     1681 ccaaaccatg gtatactaaa gaacataaag atatgagctg cgcctgtgat aagggccggt
     1741 aaggaggaat ccatcatggg aaaagaagag atatacgctt atttgacggc caagggaatt
     1801 tcctatgagg taacggatca cgaggccgtt ttcaatatgg acgaaatacg ggccgtggcc
     1861 ttgccctatc cgcagtggga tgccaagaac ctctttgtcc gcgacgataa gaagcagcac
     1921 tactacttga tttctgtcaa gggcgacaag cggatcgatt tgaaggcctt tcgcaaagcc
     1981 caaggcctga ggaacctttc ttttgcctcg ccgcatgact tgatggctat cttacatctg
     2041 actcccggtg ccgtgacgcc cttaggtctc ctcaacgatg aggcaagaca ggtccatctc
     2101 tatgtcgatg ccgactttca gggtcacctc ataggcgttc atcccaatga caatacggcg
     2161 acggtatggc tttcggctga cgacttgctc tccctcattg cagcacacgg gaatgaggcg
     2221 gagtttgtcg acgtttgatc gataagaatc tctagacaca atcgaaaaaa tgacctcttt
     2281 accggaaagc agaaaaaggg tatcatggac gtgaaataat actttttgtg taattaactt
     2341 gtctgtctct tatgacgcct tgttgcgtga tggcaaaggg tcggcctttg gggcagatgt
     2401 ctgtgttatc accgattggt gaaatcatga gtagaaatgg aggaaacgta tggaacggat
     2461 aattgtaaag ctcgtgaagg gatttaaagc gatcgatggg gcaggggtcc acctgacccg
     2521 cgtcttggga aacgctacgg tagaagcttt tgacccgatc ctcatgctcg attcttttga
     2581 cagtaccaat cccgatgact atacggcggg atttcccatg catcctcacc ggggaatcga
     2641 aacggtgacc tacctctaca aggggaagat ggtccacaag gatacgttgg ggaatgaaga
     2701 cagtatttcc gacggtgaag tccagtggat gaactcgggc tccggtatcc ttcacgaaga
     2761 acaggttccg gcgtcaccgc ggattttagg cgtccagctc tggctcaacc tgccgcagaa
     2821 agaaaagatg agccggccga cgtaccgcca tatcggcaat gatgaaatag aagacatcca
     2881 gatcgaggga ggctttatcc gtctgctggc cggagaatac aagggccgca aggcccatca
     2941 aggcgaccat ctgcctctca attactacga catccatttg gaagccggtg ctgaattcgt
     3001 cctcgacacc aaggccgacg actcggttat ggcctttaca ctcttaggtg atgccgtcat
     3061 tgccggtgag tccgtagaag ctaagacggc ggttaaactt tcagacggtg aaagcctgac
     3121 cctgaaaggg gctgaaggag gctcacagat attggtcatg cagtcacgag ctcttcatga
     3181 acccgtcgtt tggggcggcc ccatcgtcat gaatactaaa caggaattac agcaggcctt
     3241 tgaagaactg caaaataata ccttcataaa agaaaaactt gactttgacc ctcatcaggg
     3301 ctgagcctta aaaaaatctg tattcttatc ccttccggct gcggatatcc ccaccggaag
     3361 ggattttttt gcgctttttc gaaaagaaat aattgtgaat ttagtgttta gccaagtttt
     3421 tgaaggattt ttggtaaata tgtcgaaata tatatagtaa gtatttacgt aaccaccact
     3481 aagggggctt aattatgaac ggattgaaac gatgtgtgct ggccgggatg gcatcttgtt
     3541 tgctggtgtt gcctaatttt gctttagcag aggacgcttc cggcagcgag ttgatgacca
     3601 aagctgaaac aaaaacggaa gtgaagccgg ccttgccgga agacagtcat gatagtggac
     3661 tgtctcaagc ggttcagaat aaaaattatg aacaagcctt agacatcgtg aaccggcaga
     3721 ttgaagccga cactgacaat gccgatcttt atgcacgtcg cggcctcatt tatttgatga
     3781 tgggcagctc ggacgaagcc ttggccgaca gtgaaagggc gatttctctg tctccggaga
     3841 agccgtcgtt gtacctcaac cgcggctcgg tctatgaaca gcgacaggat tataaattgg
     3901 ctgtcaagga ttatacgcag gccttgacct tggcgaaacc gggcgaattc tatcaggtac
     3961 tgggctattt taaccgggcc cgagcctata ccaagatgga ggcctatgat caggccttgg
     4021 aagatattcg ccaaggcgaa aaattgatgc cctatttttc taaaaattat ttccttgaaa
     4081 gcctgattta tcagaagcaa gggaagaagc agctggcgaa gcggcgcatg gatattggaa
     4141 ccatgtatga gctcatgtac caaggccatt atttcttagc cggtgtcatg gctgaacaga
     4201 gcgatttgaa tgaaacggct ttgaacttgc tgcacaaagc ggttgaacag gagccgggaa
     4261 atcgggaagt ttactcggta cggggcttag tctatgctaa attagatcag ccggaaaagg
     4321 ggannnnnnn nnnattgccg atttaaccaa ggccttgtcc ataaaggaat cggccatgga
     4381 ctataacaac cgcggtgaat gctaccgcta tctcaagcag ttcaacaagg cccgcaaaga
     4441 ctatgccaat gccctgcgtc tggccgaaac tgattctgac taccatgccg tctacgacag
     4501 tatggggcag ctggccttcg atcagggcga ctacaagaag gccgttgaat atttgaccaa
     4561 ggccatttct atcgcgcctt atgaggatgg ctataaaacg cgcagccagg ctttccggaa
     4621 gctgggagac aatcaaaaag ccgaccagga tgaggcctcg attcaggaat tgactcagcg
     4681 cgaattgtta tcacaataat ttacttttca aattcccaca agatgtgcta taattgtagg
     4741 cgttattctt gagtgaaact gcctataata tacacatcat cgtattgaag ggggaatttg
     4801 atgaagaagg ctgttatagc ggccttgtcc atatgctgcc tcatgggcgc ggctacggcg
     4861 tcggcagcag aggtacaggt cagaacggag aaaacggtat cggcagcagt aagcgcgacg
     4921 gaagaagctc agaatgaagg cccgacgcag aaacaatatg tcccggcgaa aaatgccgcc
     4981 catcatgaag gttcgttgaa agaccgggtc catgctattt tgaacaacca agagagcgct
//
LOCUS       DAWDXZ010000076         5025 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4497_length_5025_cov_10.973441, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000076 DAWDXZ010000000
VERSION     DAWDXZ010000076.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4497_length_5025_cov_10.973441"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4735..4744
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1124 a         1514 c         1249 g         1128 t
ORIGIN      
        1 gcggcgatag agtgattcaa aggttaaatg gaggatgacc gcctcgttgt tggtgagact
       61 gcgccttttc agcagttcat acagcaaggc atcgaggtct tccatggaaa tactgaccgc
      121 cggcaggcgt tcgtggagca ataccggcgt gcggtcctcc ttcttgggcg tcagtttgat
      181 gatgcggacg aatccgccgg acccgcgccg cgactcgacc agatagcccc ggtcgttaga
      241 aaatcgcgta ctcagcacgt agctgatctg cgacggtgca cactgcagct catcagccag
      301 ctcattgcgt cgcaacacga tatcggaatt gttttcttcc aataatttat gtaagataaa
      361 ctgttcgatt ttatccgcta atacactcat cgtaaatcac ctcttaaaaa aatcgacggg
      421 tttaattttt ctttttgact ttcgtatagg tattttataa gcgaggtgtt actcttgaaa
      481 cagcggggac ccggttccgt ttattactac tttttaaaag aaaagaaatt actccttatc
      541 atcaccctaa gcggactatg ctacaacgtc ggcatgatcg ccggcccctg gttcgacggc
      601 cagttggccc agtccctcta cgacatgttc ggcggcacca aagaggcatc cgacatgtac
      661 gccctctgcc tgatatacgc cctggtcatc attgccgtcc agagtctgcg ctacgtaaag
      721 cggctgtacg tccgaaagtt tgccaacaac gtcagtctga ccatgaagga cctcctctat
      781 cagcagctgg tacagacgcc gacagcggcc atggagggtg aacatacggg aaccttgatg
      841 accaaagtca tttccgacat cgacacctgt gtcgaaggga tgcgtaaatt cacgacggaa
      901 atcttcgata cgggcgtcgt catggtgacc tacctgatca tgctcttggt gtacgactgg
      961 aaactgaccc tctgcatcct ggccttcccg cccctggctt attttctggc caatcgactg
     1021 cggcgcctgg ttgcggaaac ggctgcccgc agcaaagaaa gcagcggcgc cttgtcgggc
     1081 atcaccctgg accgcatcag caatgccctg acctaccgcg tctacggcga agaagacgtg
     1141 caaaataccc agtacgaacg ttacctgaaa gactatgaac agaagatgat tctagccaat
     1201 ctctgggaaa atacgatgca gcccatctat aaaatcatcg ctatgaccgg cacgatcctc
     1261 gtcgtctggc tcggcggccg caacgtcctc ggggagggtt ggcaagtgtg ggacatcgct
     1321 gccttttcga cgtacctctc ttgttttgtc aagatgtcca tcaagacctc ccatgcagcc
     1381 cgcctgttca atgccgtcca gaaggcccag gtgtcgtgga atcgcatcca gccccggttg
     1441 caggcctccc atcctctgcc ggaaccggta ccgacctatc cgccggcatc gctgacggtc
     1501 tccgacctgt catttgccta taaccccggt gataaaccca tctatcacgg cctgtccttt
     1561 tcggcgaccc ccggtgacat catcggcatc accggccccg tagcctgtgg caaatcgaca
     1621 ctgggcctta ccttcctcgg tgaaaagccc cacggcgggg agattttctt cggtaaccgt
     1681 cccttaggaa acgggcagac cggtgcagaa ggcatcgtcg gctactgcgg ccacgacagt
     1741 gaattgtttt cggcgagcct cgaagaaaac attcgcttcg gccttcccgg agacgtcacc
     1801 aaagtcattg ccgaagtctg tatggatgaa gactgtaaga ccttcccttt agggattcag
     1861 acaccgatcg gcgaaggagg tcagcgcctc tccggcggtc aacaggcccg tatcgccctg
     1921 gcccgaaccc ttttccatcg ccggccgctc atgattttcg acgatccctt tgccgccgtc
     1981 gacatggcga cggaacgaaa aatcttcgag cacctgcgac aatcctaccg ggactgtatc
     2041 atcctcctca tttctcaccg cctgtccctc ttttctgagc tcgaccaggt catttggctc
     2101 aaccctgacg gtacggccgt gacggcagac caccaaagcc tgtatgaaac tcatgacgat
     2161 tatcggaggc tctaccggat gcagcaagcc cagcgaggtg accgccatga ctaagatatt
     2221 ttggcaaacc gtaaggtcca atgtccggct catcgtcggc ctgtgccttt ccatcgccgc
     2281 ttctatcggc gtcaacgtcg ctccgcccct cattttgcag tacatcgtgg acagcctggc
     2341 cggcggccat tttacagccc tgcccttact gacggcaggc gtcgtctatt ttgccgtaaa
     2401 cgccggcagc ggcctcgtcg acgccttgaa agaaacgttg attaccatct tcggccaaaa
     2461 gattacccac agcctgcgca cggccatgtg ccaaaggctc agcatcctgt cggctcctta
     2521 tttcgtcagc catgaatcgg gagccgttac gtccctcttc gtcaacgacg tcgatacctt
     2581 ggaagacctc tttgactccg gcgtcgtcag catgattgcc gacgtcctga ccatcttcag
     2641 catcctcgtc gtcgtcttcc acctcagtcc cggcctgggg atcttattgt gcatcgccct
     2701 gcccctcttg tttctcctga cccgctactt ccaacagcgc atgctgaaag cccagcggga
     2761 caaccgcatc gccatcggcc ggacgaacga actcatcccg gaaacagtcc acaacatccg
     2821 caccatccac acctgccagc aagagcgcca cctgcggcgc cggtacggac agacgatcaa
     2881 ggccagtttt tccgctatgg agcgaagcaa tttctgcgac gccatctact cgcccatcgt
     2941 cattaccctg tcgacgctca tcatctgcat catgatggcc ctctcggtca gcagctcctt
     3001 cctgcaggac ctcttcggca tgaccgtcgg taccgtcgtc gccctcatcg cctatgtccg
     3061 cctcatcttc tcgccgattg aaagcatcgg catggagatc cagaatattc agtcggctat
     3121 ggccggcatc agccgcctga aagaattttt cgcccatccc gtagaagaac aaggatccac
     3181 agccgatttg aattttaccg ccccgccgct ggttatcgac gatgctgcct ttagctacgg
     3241 ccgtgaaaat gtcttccgcc acctgtccct ggccgtcaag aaaggggaaa cggtcaccat
     3301 tacgggccgc accggttccg gtaagagtac cctgttcaaa ctcatcctcg gcctgtacgc
     3361 gccccaagaa ggctcaatcc gcgtcttcgg ctgcgagcct tctcgcctgg ccccatcggt
     3421 tcggcgccaa gtcttcggct acgtagagca acagttccac gacgtcgccg gtaccatcga
     3481 agaccaagtg accctcaaag accctcgcgt ctcggaagaa gccgtcgaaa aggctctgcg
     3541 actcgtcggc ctgtgggatc tctgcacttc cctccccgag ggacttcaca cgcccttccg
     3601 acaagaatat ttctcccgcg gccaactcca actcctgtcc atcgcccgag ccgtcgtcat
     3661 gaatccgcaa atcctcttac tcgatgaaat cacggccaac ctcgacgcct taacggaaca
     3721 acaggtactg acagccctta aggctgcctc ccaaaaccgg accgttctgt ccatttctca
     3781 ccgcctctac gaagcccaag gcggccgcct cgtcgtcatg ggcaccgccc aaaagggccg
     3841 tccttcgtaa gacactgcca caaacaactc cacagactca cctgataaag cgcttctccg
     3901 gattttggcc cgtgggagcg cttcttggct ttgcagccgc cggaatcgga cgacgtcagt
     3961 gacaagacgc gcctttccat cctggccaaa gtctatgaag ccttagaaga agcacgaaaa
     4021 aaagccagct cccggaacgt atgaatacgc gggaactggc agaagatctc tgcgtcctgg
     4081 aaaaaggatg catttttgaa taggccctct cagacggccg ttacccctta tgggatagaa
     4141 gcaaaaggtg ctttgtgctg ttttaaaagg gatggcggct actgattaaa gatggtcagt
     4201 caccccttcg gcgcctcgat ggggtcgtaa tcggccggtt tatcgacgat ggacgcttca
     4261 tcgacggcag acggagccgg atcaaaaccg tcgcggaaca gcttgtgaac agcttcgatg
     4321 tacggcggaa caatatcatc catgaggaga taccgcttgc ggaagagatt gcgcttctgc
     4381 gacttaattt cgtcgtactt acggcgcttt tcctcaatgg gaaggcggaa ataccacgat
     4441 tcgcttttat cgggcacggc cccttcccca atccagaagt cattgaagtt ggtcttcttg
     4501 gaacggttgc ctcgcagcat gtggctgttg gtatagaagc cgtcgtcagt gatgacgtac
     4561 atatcgcgga tgcccaaggt ctggacaaag atacgcatca aatagaggat gaaattctta
     4621 ggccggtagc caaagagctt cttggtcagg atcttggtcg tttccaggcc gtgtttcgag
     4681 ccttggatgg taccgatata catggacggc gtaccgtctc cgttgtaatc gaagnnnnnn
     4741 nnnncggaag ttaaaatggt agatcatctg accttcatga tagagataga gcgacaagaa
     4801 cccttctttt cgctggcccg tatcaaagat gagccgcact tcaaggggca tggacggatc
     4861 gtcagacgtc cagagcgtat agcctttgcc ccaatagatt tcgcggatga catcatcgga
     4921 aaagaaggtc cgcaaaatcg tgaaatgatc cttcaaggcg tcgaggcgtt ggcgcatggt
     4981 cgaatttttg aacaagaaga cccgggtcat gacttcctgg aagtc
//
LOCUS       DAWDXZ010000077         4997 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4523_length_4997_cov_9.559085, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000077 DAWDXZ010000000
VERSION     DAWDXZ010000077.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4523_length_4997_cov_9.559085"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3174..3183
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4579..4588
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1340 a          964 c         1310 g         1363 t
ORIGIN      
        1 gctgttgctg gagatggata aaatggccga acatgaattg aagtacatgt caagcgaacg
       61 catgtatgaa ggtattatta ttgaagtaca tggcggcagc gttgtcattg acttaaaagg
      121 acgtctgggg cagcttcgga ttcctaagag gatgatcatt tccgactatg acttagaagt
      181 aggacaagaa gttggatttt tgatgtccta tccggaagtg tttgagcaag agccgaacga
      241 taaatacgtg cgagcaatcc gcaaccagca gcgtcttatg agagaacgaa aaaaagatat
      301 ctaattctga ttaaaggagg aaacgcctta tgcgtgtaac tgtatttgaa ggcttacagt
      361 cagaaatcta tgtgccgatt acacctaaat ctatttttac acccgtgacg aagcccttga
      421 gtgaaatgcg aattgccttg gcttctgcct gcggtgctca tgttaaaacc gatcctcgat
      481 ttaacttagc cggggatacg tctttccggg aaattcccga taatactccg acgtctgaac
      541 tcatgatttc ccacggggga tatgacaata cggacgctaa ccgggatatt aactgcatgt
      601 tccccctcga cagactccat gagttggtcg aagaaggatt tattaaagaa acggcgccct
      661 tccactacgg ctttatgggc ggcggcggtg accaggaagt attccacgat aaaacgggtc
      721 ctgaaattgc ccaaagattg aaagacgaaa atgtcgatgg cgttatctta accgctgggt
      781 gaggcacgtg ccatagaact gccgtgatcg tgcagcgagc cattgaagaa attggaattc
      841 ctaccattat tattgccgca ttaccacccg tcgtacgtca aaacggtacg ccgcgtgccg
      901 tatctcctcg tgttcctatg ggggccaatg caggggaacc gcataatata ccgatgcagt
      961 tgggcattgt acgcgatacg cttcaggcct tggaagacat tcagacgcct gggaagatcg
     1021 tgcccttgcc gtatgaatat attgcacata tttaataggc atgattgact gaaaagcagg
     1081 tgggataatg cgaggcgaat cgttacgtat acgagtattt cacatgcagg atgttactgt
     1141 gggagctcgt tttgagctga aaccggatcg tttacaagtt ccaacaacat tctcttacga
     1201 ttgccccgac attgaacaac tgaccattca tgtcttgcca ccgcattgtc gggatctcga
     1261 aacgaatacg attatggatt ttattcctat atcgacaaag gttttgggaa ctcccggtac
     1321 aggcattacg cataccctga ccggggtatg cgtggccctg tgcggggcga ttcgtgatgg
     1381 cgaacagatg catgagttcg ggtcgacaga aggcctatta aatgatcatg tcaaatgggg
     1441 acgcccgggg acgcctaaag aggatgatta cctgatcgta atcgatatgt tggctaaaaa
     1501 agattgtatt ttaacccgaa agctttgctt ggaaatgttt gctcttgctg atgattatat
     1561 ccagcatata cgggtgatgt tgaaggaaat ggatgggcgc gatgctgtcg aaacccatga
     1621 atatgaagaa gtatcgcatc cgggaaaacc gaaagtagca ctcgttcgac aagttgccgg
     1681 gcaaggtgcg atgtatgata cgatggtctt tccttttgaa ccgagcggat ttgcgggagg
     1741 agtttccatt attgacatga ataatatgcc tgtttttctt acggccaacg agattcgtga
     1801 cggaatgttg cgggctatgg tctagagaaa ggaggcgtaa tcatgggtat aggtccttct
     1861 acgaaagaga cgacgaaaca ttattttgaa gaccccttag taaacgtctt tgcaggagat
     1921 cctgatattg actttgccgg tgttattgta gttggaaccc cgcaggataa ccggatgaaa
     1981 catcttgtcg gacagcgcgt ggctgcctgg ctggaaggga tgcagtgcga tggcgctgtg
     2041 gtctccgcag acggatgggg aaattcagac gttgattttg ttaacacact ggaacaaata
     2101 ggacgacggg ggataagcgt tgtcggtctc aaatttattg gcaagcaggc aaactttgta
     2161 gtccaaaatg aatacaccaa gcgtgtattg gacatcaata agtctgttga cggcagagaa
     2221 accgagatca tcggtgaaaa tgccgttgcg catgtcgatg ctattaaagc cctagctgca
     2281 atcaagttgg ttatacgaaa acatgccgct ttagaaaacg ggaggtaacc ttatgattgt
     2341 acacattttg cttggcttat tagctgtctt attgatttat tttctgtttt tctatattcg
     2401 cgatttatac gcacatcgcc atgatctggg tcatggaagc tggattgtaa attgcatcat
     2461 cggctttgtg gtagactttt ttgatgtttt cgggattggg agctttgcaa cgtctacgat
     2521 gctgttgaat gtgaccaaac agttggaaca tgaccgattg ctgccgggaa cgttgaatgt
     2581 tgcttttaca gttcctgtca tgatggaagc gtatatcttt attaaaaatg ttgaagttga
     2641 accgcttacg ctgttttcgc ttatctttgc agccattgca ggtgctgcta ttggcggccg
     2701 gttggttccg aaacttccgg aacgaaaagt tcaactttat ttaggtattg cccttttgtt
     2761 tacggctatc cttatgattg gcaaacaagt tgggttcgtc gatttcttgg gaagcggtaa
     2821 tacggctgtc ggcttagatg gtgcgaaatt aattattgcc attgttttga actttgtttt
     2881 tggtgctttg atgacattgg ggtgcggttt atatgcgccc tgcatggtaa tggtctttat
     2941 gttaggcatg aatccgattg tcgcttttcc cgtcatgatg gggtctgcgg cggcattaat
     3001 gcctgttgca ggtaaagaat ttattgcagc aggaagctat gcaagaaaaa tcagcttgtg
     3061 ctttaccctg acggggatag tagctgttct tattgcgacc aacatcattg tcaatatgga
     3121 tttgaaactg ctgacatgga tcatcattgc tgttgttatc tataccagtt tcannnnnnn
     3181 nnncacatat gtccgcaaag gcttaaagaa ggccgtagcc gaataattta taaaggggat
     3241 atctcgctta gaggggacat cccctttttt atgtataaag tcggggaagg atgatgtggg
     3301 atggcatatt tagtaattgc aactgcggga cacgtcgacc atggaaagac gacactgatc
     3361 aaggccttga cggggattga aaccgacacg acggccgaag agaagaaaag aggcctgtct
     3421 atcaatctgg gctttgctta cttggattta ccgaatggac aacgggtcgg aatcgtcgat
     3481 gtccccggac atgaacgatt tataaagaac atggtggccg gcttgccggg cattaatctg
     3541 gtgctgctcg ttattgatgc cggtgaaggc gtaatgcctc agacgaggga acacatagac
     3601 atccttaccg ttttaggcgt tcgtgatttt attattgtat tgacgaaggt agataccgtt
     3661 gatgcagaac ttcgggattt ggtccgggaa gatattcgag aacagctgtc tgataccgta
     3721 ttgaaggatg ccgatatggt agagaccgat gctgtcagtg ggaatggtat tgaaacttta
     3781 aagaccaaga ttcaagaatg ggcagaacga aatccggcta tggaagaaaa aggggaagct
     3841 cgcatgaatg ttgaccgagc ctttagcgtc aaggggttcg ggacggttgt aaccggtacc
     3901 cttttagatg gtacggtagg gattggtgac gagttatatg tatatccgga acttaaaaag
     3961 acgaaggtac ggaacataca aatccatgaa caaaatgtga agcatgccgg ctctcggcat
     4021 cgtactgcca ttaatttagc caatattgga cttgaagata ttcgacgcgg cgacgtcgtt
     4081 tcgtcatcac cgtctttaaa agaatcgtgg atgcttgacg tcaaagttca gtgtttgaaa
     4141 acctctccgg ttgcgatggg cttatgggac cgcgttcgtg tgctgatcgg tacacgggaa
     4201 gtcatgggac gaatcgttcc tatcggagaa gattgtattc agccgggcca tgaaggcttt
     4261 ttgcagctgc gaatggaaga gtctgttttt gtgaaaaatg gagatcgatt tgtcctgcgc
     4321 tcttattccc cgatgatgac gattggtggt ggtgaagttc ttgatgcggc tccgcataag
     4381 catcggaggt ttcaccaaga tgtactggat agtcttaaaa ctaaagagga ggggaatctt
     4441 gatgcgatta ttagcgattt tctgatcaat actaaaaata tcgtagccac gaaggatgac
     4501 ttggctgcat atgttcatat atcgaaagca gaagtagcag acactgttaa ccgtatggtc
     4561 acagagggga tcctcatcnn nnnnnnnnct cttcctttaa gattcaattg acggaggcgc
     4621 aaaagaagca gctgaaacaa ttgcagaaca tggttgatga cgctggattt aatccggtga
     4681 aaattgatga tggaatacta ccgggaacag atattgcgga actggcccga ttgaaacaag
     4741 gggaagagtt catattcttg acccctgaat atatcattgc caaaaaaagg tatgaagagg
     4801 ccattgatat tatctatgac taccttaatg aacatgggaa aatgacgttg ggggattttc
     4861 gggacgctac gcattccagc cgaaaagcat ccatgttaat cctcgagttt atcgatgctc
     4921 gtcatattac tgcgagagag gaaaactatc gtattttcgg accggcggcg gataaggggc
     4981 gaattgcatc acctgat
//
LOCUS       DAWDXZ010000078         4973 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4533_length_4973_cov_11.340789, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000078 DAWDXZ010000000
VERSION     DAWDXZ010000078.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4973)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4533_length_4973_cov_11.340789"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3235..3244
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3722..3731
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1118 a         1331 c         1258 g         1246 t
ORIGIN      
        1 taaggctgcg gtaacctcga acgcgcgacg tttcgcggcg atgataagaa atagtaacgg
       61 cctcatccgt tggcagggtt cttaaaaagg acaggagctg ctttcggtag cgataggtgt
      121 cgacgagctc acccatagcc ggcgtgtaag ccccggccat gacctgcgat gcgtcatcaa
      181 ggtcttcggt ggcctgaagt cccgttcgta tacacgagat tcctcgttcc agaaagtgct
      241 gcttcatata agccgcccgc cgtacacctt catcgatggt aagcggctta tatcgccctt
      301 cttcatacat cgtggccagt tccgtcccct ttaggacggc gacgggataa atgcgggcta
      361 tatcgggctg gagacggcaa atcgccttcg tcgttcggca caggctgtcc caatcttccc
      421 cgggaaggcc cggcatgagc tgatgaccga cggtaaagcc atattgccgc agcaaggccg
      481 tcgccgcgac gacgtctgct gccgtatgac ctcgctgggc ctcctgcagc acccggtcgt
      541 ccatcgattg gacgccgagt tctacacagg tcagtccgta gttttttaaa ttcgttaaaa
      601 tatcttcact gatgcagtcc ggccgcgtcg aacagcgaag gccctgaata aggccttttt
      661 cgagagcttc ataagccggc cggagcaatt ttttttgcat ctccatggga atggccgtaa
      721 agctgccgcc gtaaaaggcc acttcccagt gacgcccttc accggctgag gccgtatagc
      781 cttgaataag ctctcgtatg gcacctgccg aaggcaggcc atgatggccc gtaatctgcc
      841 actgattaca gaagacacag cgatggggac agccgatatg ggggataaaa atagggatga
      901 tagaagtttt catggtcata aagtgtataa attgcgaaag aggagctgct caggcagctc
      961 cccgtctact tgccttttaa ccgcatgagc gcttcgtgcg ccgcgtgttg ttcggcatat
     1021 ttttttgatt ttcccgatcc ttcaccgagc accttgccat tgaccagaat ttccatgtaa
     1081 aaagttttat catggtccga tccttctgtc cggcaaaggc gatactcgat atgctgcgga
     1141 ccgtcttgct gtacgtactc ttggaaaatc gtcttgtagt ctttttcata atgaccactg
     1201 tcgacgaggt ccaaatagtc ctttaattgg tgaaggatga atttccgcgc ctcttcgtaa
     1261 gagctgtcga tatagatggc accgacaacg gcttcaaagg catcggccag catactggga
     1321 cgcgtgcggc cgccggttgc aagttccccc ttccccagga ggagatagtc acccatatgg
     1381 aacttcatgc acaccgaagc cagggggctt tcactgacga cactggctct ggcctttgat
     1441 agctgccctt cgggcatatg cggatagtgg agaaacaagt attcccctac gaccaagtct
     1501 aaaatggcat cgcctaaaaa ctcaaggcgt tcattgtagt agccgtgatt ggctttattt
     1561 tcattggcat aggaagaatg ggtcagggcc tggtccaaaa taaggaaatt atggaactct
     1621 ccaaggccca agacctgtat gaactgttct aactgttctt tacgagtcgt atccatcagg
     1681 tcagtcttct tctttattca gcatattttt tgaacagcag cgtcgcgttg tgaccgccaa
     1741 aaccaaagga gttggagatg gcaacggtga cgtcggcgtc aacggcttta ttcggaacat
     1801 agttgatgcc aaaagctttc agttcctctt ccggttcaac aaggttgatc gtcggatgga
     1861 ccttgccggt ttcaatcgtc atggcgcagg caatcgcttc gacggcgccg gcggcaccga
     1921 ggagatggcc ggtcatggac ttcgtcgagc tcatgagcat atctttggta tggtcgccaa
     1981 agacacgggc aacggcgacg gattcattaa agtcattgag gtgagtggat gtgccgtggg
     2041 cattgacgta gtcgacgtct tccggtttga ggccggcgtc tttgacagcc agggccatgc
     2101 acttggactg ctgaaggcct tccggtgccg gtgccgtgat gtggtaagca tcggcattgc
     2161 tgccgtagcc gccgatttca cagtagatgt gagcaccgcg ttttttagca tgttccagtt
     2221 cttcgagaac gacgatgccg gcgccttcgc ccatgacgaa gccgtcacgg tctttatcga
     2281 aaggacgcga agcatgagtc ggatcgtcat tgcgcgtgct gagggccttc atcgccgcaa
     2341 agccggcaac tgctcccgga gaaacggcag cttccgtacc gcctgccaag gctgcatcga
     2401 gttcgccccg ctgaatcatg cggtaggcat cgccaatgga gttggtcccc gttgcgcagg
     2461 ccgtaacgac actggcgcag tggccttgaa ggccgaagaa aatggatacc tggccggagg
     2521 ccatgttggc aatcatcttc ggtacgacaa agggattgac ccgggacggg cccttcgaaa
     2581 agaggccttt atataagtta tgcagcgttt ccatgccgcc gataccggtg ccgataatcg
     2641 taccgatacg gtcgcggtct tcttcgtcta agttgatttt cgaatcatct aaggccaatt
     2701 ttgcagcggc tactgaatac tgcgtggacg ggtccatgtg acgggcttct ttccggtcga
     2761 taccgaaatc ggcaggatca aaatccttga cttcgccggc aatgcgagtc gtatattccg
     2821 ctgcgtcaaa gtgggtaatg gggccgatgc cgctttcgcc gttgagcagg gccttccaaa
     2881 aagcatcttt accgatgccg acgggagaaa taacccctaa ccctgtaatt actacgcgtt
     2941 tttccaaagt tttcacctca ttataaaatg taacgcactt atcactcggg aatctcttcc
     3001 atttcccgct tgatatttgc aaatatatcc ttgaccggca ggatgtcatg aatttccatc
     3061 attcgtttgc cggaaaatac caggcctgtt tcaacgtccc cttgctgggc ccgcgtcaat
     3121 gcccggataa tacagtattt atgactgcac tttttcagac agaaatcaca cgtttccggc
     3181 ttcggtgccg tacccaaaag gattcgttcc gagaacggcg tacggatagc ttgannnnnn
     3241 nnnncttccg gtttattggc ccggaggtag acttctttta aagcgtcagc gccgtttgat
     3301 tcgacactgg ctgcaaatcg gctgcccatt tggacgccgt tggctccgag acggagcatt
     3361 tctgcaatat cgcttccatg caggacgccg ccggcgccga tgacaggaat gtcaacagcg
     3421 gcccgtactt ccggtacgat gacgcggctc gagcgattcg ttcccaaatg cccgcctgct
     3481 tctgcacctt cgacaacgac ggccgaagca cccagggatt gggatatttt agctagtttg
     3541 gcagaagaca caatagggac aataggcgtg cccgaatcct ttccccaagc aaacatatct
     3601 cgggaaaaac ctgcgcctgc cacaaccaga tcgattcctt cttcaattgc cgtagtaacc
     3661 aagcccttaa attcgcgggc tgccaccatg acatttatac ctataattcc acgaggagat
     3721 annnnnnnnn ntgtcgcaat tcgtcaaaag gcatgccaga tgccgcaata aggccgatac
     3781 cgccttcatt ggctactgct gccgccaaac gggctgtgga caaacggatt gccataccac
     3841 cttgaataat tggcaccttt gctacaagct tacctattct taattctggc agcttcactc
     3901 tgttttcctc cattcaagat accaggaggg gaagacacat atgcctcccc cttcaggctc
     3961 ttattgcaaa cttatgcttt cttttccttt tcaatgtaat caactgtatc tttgacagtt
     4021 ttgatttttt cagcaacatc gtcagggatt tcgatattga attcttcttc gaaagccatg
     4081 atcagttcta cgatgtccaa ggaatcagcg cctaaatcat cgatgtaagc agcgtccatt
     4141 ttgacttctt tttcgtcaac gcctaactgt tcaacaacaa tgctctttac tttttcaaaa
     4201 gtattttctt cgctcatgtc cattcacccc ctttcagtgg agtatgattg tttacattac
     4261 catgccgccg tctacattga gggtctggcc ggtaatataa ctggcatcat ccgatgcaag
     4321 gaacaacacg gcttttgcca cttcttccgg ctgagccacg cggcccatgg gaatcgtttt
     4381 aatcatggca tcgacagtgt cctcgccgag gacagccgtc atatccgttt cgatgcatcc
     4441 cggagcaatg gcattgcagc ggatgtttcg cgaagccagt tctttggcaa ccgatttggt
     4501 gaaaccgatg aggcctgctt tcgccgcagc atagttagcc tggccggcat tgcccgtttc
     4561 accgacgaca gacgtgatgt tgataatcga accgctgcgc tgcttcatca tcagccgggt
     4621 aacggctttg gtgcagtgaa aagcgccggt aagattggtg tcaagcacag cctgccagtc
     4681 tgcttctttc ataatcatga gcaggccgtc gcgagtgatg cctgcattat tcactaatat
     4741 gtcgatacgt cccaattcct tcttgacgtc tttgaccatg gccgtaacgg cggcttcatc
     4801 ggccacatcg cactggatgg caaggccctt acgccctttt tcttcgataa ggcggaccgt
     4861 ttcttcagca gcggcggtat tgccggcata gatgacagcg acgtcggcac cggcttcagc
     4921 aagtgtcaaa gctgccgcac gaccgatgcc gcgggagccg ccggtaacga tgg
//
LOCUS       DAWDXZ010000079         4915 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4571_length_4915_cov_9.212346, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000079 DAWDXZ010000000
VERSION     DAWDXZ010000079.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4571_length_4915_cov_9.212346"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1264..1273
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1865..1874
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3410..3419
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4361..4370
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1288 a         1253 c          981 g         1353 t
ORIGIN      
        1 gatcaacgtg ctatactatt ggtatagcat gaattattgc ataacccgag gacgcccatt
       61 ctcaaaaaat gcaatgtttt catgcttttt gctatatatg cagcagtata gatcagcctt
      121 ttacttgttc catcgcttat ggtcgtatgc tttgggattg cataccttat cccacttttg
      181 accgtttata acagctaccg ttccttctgc cgccagggtt gcaacacctg aagccgcttc
      241 tcgcgtctgt cctgccatat gaggggtaac aacgatatta tcatgatgga aaataggatc
      301 tgccggatca atcggttcat gtacgacaac atcagtcccg gcactgtgaa ttcggttttc
      361 atctaacgct ttattcagcg cagcttcgtc aacgataccg ccgcgagcac agtttatcaa
      421 aatgccatct tttttcatca tctgcaattc cgcttcccca atcatattcc gcgtttgcgg
      481 cgtcagcggg gtatggatgg aaacgacatc ggattccttt aagatatcat gcatatcttc
      541 acgatatcca tagccttgtg cctcaaccgc ttctcgttta ataaacggat cgtaaaccaa
      601 gactttcatg ccaattccgg cagccatgcc ggcaaaaata cttccgatat ggccgtaacc
      661 caacaaacca agagttttac cgcctaactc ataggcttta taggagctgc gaatgccaaa
      721 gttaccgtta cgcatttctt tgtcactgcg aatcatatct ttagcgcagg caaacatcat
      781 tgccatcgca tgttctgcaa cagaacgact attggcccct ggggcaatga cgacgggaat
      841 tccgagttcc gttgcagctt caacatcgac atcgtcaacg ccgacaccgg gtcgtccaat
      901 aactttcaat ttcgggcact gttccatcgt ttcccgatca atagaaccga ttcgaatgat
      961 aacaccatca gcatctttca attccggaag cataaccttc gggtcgccgc tatttgtaat
     1021 aacgacctct acaccggcct tttcaagaac agccatgcca tcgtcgtgca gacgatgtga
     1081 aattacaact ttagccatga attacatctc cttacgtcaa ttattcatac agtttaaaaa
     1141 catcttccaa ggccttttta atagccggat ccgtgcagtt cgacggttga cgagccggac
     1201 ctacgtgata accgcgcatg ttcaccgcaa gttttacgac agagttcgga ttacccatct
     1261 gcannnnnnn nnnatgacgt ttcggaaagc gcgaattttc ttctgcgatt cgttagcttt
     1321 ttcgatgttg ccttctttcc agtagttata gatgctgacc atcagttcgg ggaagacatt
     1381 ggagcagccg gaaatggcgc ccgtgccgcc tgccatgagt gcccaaagaa tcagggaatc
     1441 actgccggcc agtacattga gacgaggatc ggtattttca atatatttca gcatattatc
     1501 gaaattgccg ctcgaatctt taataccgat gatattttca tattttgcta aggttttaac
     1561 cgtcttccag tcaatggcgt tacccgttct tgccggaatg ttatacaaca gaatcggcag
     1621 gtcaacggct tccgcgacag cgctgaaatg ttcgatcaag tcagcctgag aagcttttac
     1681 aaagtacggc gtgatgatgg acaggcaatc gataccgccc atctgctgca cagccttcga
     1741 catttcgatc gtttctttcg tcgtaacgca gcccgtaccg gcgtaaacgg gaacccgacc
     1801 ttgaacggct tccacagcag cttcaatgat ttccaatttt tctttgaaag aaaatgcata
     1861 gaacnnnnnn nnnnggaaaa taccatggac gccggcttta atcagatgat cgatgaacgc
     1921 ctggtattcc tttttgttaa attcttcgtt ttctgtcaac gccgtaacaa cgggggtaat
     1981 caaaccttct gctttaaaca taatatattc gctcctttgt gcatctatag aaatttaatg
     2041 atctgcaaaa cgtttaccta aatcaacggc ataacacata ctgtctgcct tggcgatgcc
     2101 tttaccggca atgtcaaagg ccgtaccatg gtcgacggag gtacgaatga tgggaagtcc
     2161 taaagtgaca ttgacaccgg aatcaaaggc caccagcttc atcggaatat gtccttggtc
     2221 gtgatacatg gcaacgacaa tatcgtaaac accgcggtaa gccttcaaga aaaccgtatc
     2281 cggtgaaacc ggtccgtcca cgcaaagtcc ttctttcttt gcttcttcaa tagccggtat
     2341 gatttcatca atatcttcac gtccgaagat tccggcttcc ccactgtgcg gattgagtcc
     2401 ggcaacggca attctcggtt ccgtaacgcc taactgtttc atcgcaccat cagccaaatg
     2461 gatacattcc aaaattctgg ctttctttgc tcgattacaa gcttctcgca gagaacagtg
     2521 cgtcgataca tgaacgaccg acatcttttc actccaaagc atcatggtat aggctttggt
     2581 gcccgtaagg gttgcaaaga tttccgtatg accgtcaaat tgatgcccgc ctttatgaag
     2641 agcttcttta tttaacggcg ccgtcgtgac gactttgatg tcacccttca aggcatccga
     2701 aatggctctt tcgatatatc ggtaagcagc atctccggca acgggagaaa cctttccgat
     2761 ttcaaaatct tttccgatgg ccaagtctat aacagagata acattaatct ttcccggctg
     2821 aaattcttga atattctgaa tacgtaccag cggcgtcgtc aagcggagct gttggtggta
     2881 gtattggagt acatcatagc tgccgtagat caatacagac tgctgatact cttttttagc
     2941 atctatggct ttgagcgaaa tctccggtcc gattccggcc ggatccccca tggtgatgcc
     3001 cacaaattta tttgccactg tatctcctcc tttatcacaa catcgttgtt ttgttatgtt
     3061 gttatgtgtt tatttttgtt gtttaaatta tatttcactt gttcgaataa gtcaacaact
     3121 ttttataatg tcaacaatgc ctatttatca ttgttttttc tttttaattt gctgaaaatt
     3181 cttaaaaagc aattgctaag gctagatagt cagtggcttt atccccattt atccgcctct
     3241 ttaaatcatg atattttaac ataatagaac agataagaac aaaaaagcca ttagaatagg
     3301 caacattcct tacctattct aatggctttt aacgatttct atgcaccgaa aattcacggc
     3361 tccggcagat atcttaaaga ttctatacaa ttgtaaatat ctttttccgn nnnnnnnnna
     3421 atgggcaggc catttcgaac accgcccaac agtctccctt gaacaaaacc gggcacaagt
     3481 tcccgttcaa tccgggatcc gtgtgctgcc aaccggcgtg tgacagcaat ggccgtatct
     3541 ccgcccgtca aaaacagtcc gcttacggcg gcatccttta aaacagccgg caccagttga
     3601 ctcaaggttt gtttcgtaaa ctcggccagc gaatccgtag aaagcccctt ttcacgacca
     3661 taggcagcaa aatcttcgta cgcctgacgg cagtccgctc ccgtcaaaat gacattatga
     3721 ccttcctgta aacgtgagac aaccgattgt ataacgtcac cggtgcccgg cacttcataa
     3781 agttctttca tatccaaaga aacgatattc actcccttat ctcgacagta tgcaatctgc
     3841 gccatcgtct tgctgctaat gctgccgatg actgccaata ccggaagact ccggcaactg
     3901 acctgtaata tcccctgagc gagaccggct gaaccgatcc aaaggatttt ctttcccgtc
     3961 ttcatggccg ccatagcaat atgctccaac tgtgccggct cctttgtatc aaaggaatag
     4021 gctcgccccg tcaaggcaat cgctccctca gccagatcat gatagtcata atgcagtacg
     4081 ggttctgtca ggcaggccga caggatcttc tgaacattgt cttcttccaa agggtttctc
     4141 ggatcacggg cgatttccgt atcgagaagc ggcgtcccct gaacgcaaag acggccttgt
     4201 tctaccgtcc gtcccatttc gggataagcc ggcgcaaaaa tgatgagttc cggcccataa
     4261 ctgtctaaag cagcacggat ttcttcttgc aaatgaccgc gcagggtcga gtccactttt
     4321 ttgtacaaaa agcgacaccc cccttgccgc attacctgcg nnnnnnnnnn ataagcctct
     4381 tgaggcgata cgacgcggct ttcactatca atagccagac tattttcatt atcttcaatt
     4441 tgatctatat ccaggataac atcagcggga atgccccgtt tacaaagctg gacaccagta
     4501 tcattcgccc ctgtaaaatc atcggcgatg ataacaaaat gatgcatact accacctcac
     4561 tgcacatgaa taaagtttac ctgatgctcg tcaaacagcc cttgttcctc agccgtcgcc
     4621 gtataatcac tgatcaaggt gtcaacttcc cagatatcga aaatataata gagtttatgc
     4681 ttcttaaact tagaatgatc ggcaaggagg accaccttat cggacgcctg caaacaagct
     4741 cgtttaacat caattttttc agcattggcc gtcatgattc gccagtccgg cgtaatggcc
     4801 tgtgtcccaa aaaagcagat atcaaaatgc atggtctgca ccatattggt cgccaaaaca
     4861 ccgcaggtcg aattggattc gacgtcaata tgaccgccca aaagaatgac gtata
//
LOCUS       DAWDXZ010000080         4891 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4591_length_4891_cov_9.533706, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000080 DAWDXZ010000000
VERSION     DAWDXZ010000080.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4591_length_4891_cov_9.533706"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1169 a         1252 c         1210 g         1260 t
ORIGIN      
        1 gttccccata tcaacagaac catcagaaac ctgtttcaga cccgtaccgg aaccgccggc
       61 attgagggta atggtgacgt ctttgttcgt tgccttgaat ttttcagctg cgtctttaac
      121 taaaggcagc aaggccgaag aaccagagcc agtaatactt ccggaaacgc tcgcttgttt
      181 ggacgaatca ctactctgct gctgacttcc gcaaccagcg acgccaatag ccatagtggc
      241 tgccaaagcg atgagaacta attttttcaa cttcataacg aaatcctctc ctttaaataa
      301 aagaatctga aagatgttcc tgattctgaa tacagtctta tcctatcaac aggatgtaag
      361 gtttggatgt atgtattgta aaatattgta aaaaaatcac cactccccgc caggcctgat
      421 caggcctgga aggtaatggt gatttcagtt ccttcgtttt ctttactggt aagggcaatc
      481 gtgccgtgat gctgttcgac aatgtgtttg acgatggcaa gacccaaacc ggtacccttg
      541 attttctgag agcgactgga gtcagcacgg taaaagcgtt cgaagactcg cttctgctta
      601 tcttccggaa tgccgatgcc cgtgtcgcga acaatcaaaa cggcacggtg accgtcatga
      661 cggacggtaa cgtaaacgtg accgcccggg cggttgtact tcacggcatt gtcgaggagg
      721 ttcataatga gttcccggca caggccgcgg tcgccgagga tcgtcgtatc atccatcgta
      781 caatggatgg ttaccttttt ctccatgagg accggttcca taaattcgac gatttcttga
      841 ataagttcgc gaaggtctac gtcttccatc tttatctggc gtttaccatc ttcgatgcgg
      901 gccaggtgca tgatttcttc aatcatctgc aagaggcgca gggcttcttt gcgaatgagg
      961 gtaccaaaat ggacgatatc gtccttatta gtacagatcc ccgtcgacag cacttcggca
     1021 aagccgctga tcgacgtgag aggtgtcttt aattcatggg atacattgga cgtaaattcc
     1081 cgccgcagct gttcccgccg ttcttgttcc gtcacgtcgt cgacgataaa cagcatccca
     1141 taaaaatcat catctttata gaccggctga atcgtcaaga gataacggcg gtccgctcgg
     1201 atcaattttt cttcgcagac cccttccaaa tgctgcaagt ggctccacgg cgcatcgggc
     1261 atgagttcct ttaagttgcg ctgcagcagc gcctgggtat gatcggcgcc taaaaagtga
     1321 gcagccctca gattgagacc ggcaatgccg tacgatttgt cggtcaggat gacgccttcc
     1381 tgaaggtttt ccatgataag gcgcatcatg ttccgctgct gctccaaact tcgcaagttg
     1441 tcggtaatgg tctgactttg gaagaagatc ttatcgacca agggctgcag ctcatgatcg
     1501 accgacggcg gttccgtcat cttaacgtta acatcattga tttggcgaat aaaaagtgcc
     1561 gtctgccgca aaggactgag aagactggct gtcagaatac gcgacgctct gacacaggcc
     1621 agggccgaca gacctaagag aagcagagtc caaggaagga ggttcaagaa gtgggcataa
     1681 aaagtatccc gctcgagact gacccgtaaa atcgttccgt caggcaatcg cttggcatag
     1741 tagtacaagg ccttggaaac ggtctgcgaa tcgcggacgg ccatgccttc cccattggca
     1801 atagcggctc ttacctcagg acgttctaaa tgattttcca tgacgccctt atcataatct
     1861 gattcaaaga ggacgacacc atacgtactg atccaggtaa tgcgcaggcc gtcattatta
     1921 tgaaagccca ttttttgcag gtacagcgtc gtatcattgc cttcttcaat cgtcgtttcc
     1981 acgacattca tcgtcatttc caattcctgc tggatttggc gttgcgtaga gttccaaaaa
     2041 aaccaagtcg atatgagcag cgtgacggcc atgcaggcca gccccatgac cagcaggctg
     2101 gcgtagacct tacgcttcat aggccgcctc cgatgacata gcctacgccg cgaacggtat
     2161 ggatgatctt gccgccgtcc ccgagtttct gccgcaggct cttgatatgc atgtcgatgg
     2221 tccggctttc cccttcatag gtaaagcccc aaacggcttc catgatctgt tctcgtttca
     2281 aaacgatgtc cgtattgacc aaaaggtacc tgagaagttc aaactcctta acggtcagat
     2341 gacagggctt cccgtcgacg gaaacggtat gtttttcctg atcgagggtc accaagccat
     2401 aggtatagac gttggtttcc gtactctgtc gtgtcgaccg gcgcaggacg gcgcggatac
     2461 gggaaatgag ctcgacgatt ccaaagggtt tgcagacgta gtcatcggca ccgccgtcaa
     2521 ggcctttgac gatatcatat tcgctggtct tggccgtcag cataatgacc ggcagagact
     2581 tatactgcgg cgatttatgc agtttgtcga ggatgtcatt gccgctttca ccgggcatca
     2641 tgatatcgag cagcaacagg gccggcatcc gattttcaag agccggccaa aattcccggc
     2701 tgtcggcaaa ggtttctacc tcaaaatcct ggctgcgcag ggcataaccg acgagttcac
     2761 ggatattttc atcatcttcc acacagtaaa tcaaaggcat aggaacacat tcctttcata
     2821 aaaacaataa ggctatttta tacttattat aacctaatcg cgagaaaatc ggctgtaaaa
     2881 ttaccgtaaa actacaaata aagggactgc cttcatcgga cagtccccct ctatagcttt
     2941 ttacttccat atcttacaag tcaaagtcct tagcctgtac cttaacttgc cactcttttt
     3001 caacctgctt gtaggtaatg atggcgataa aagagtggag gtcgcggacc cacgtataat
     3061 tccgggttgc aatatcattg ttatcgagat tctgagccaa ggagaagatg accgtatccc
     3121 gcggggtcag gacatatccg acggaaaagc ctttctgtcg cggcttgtcc agtgtatcga
     3181 agcgatacgg tgtgtagccg ctgacattat gcttcttgta ccagaaactg gtccacagac
     3241 gatcgttgat gccttggttc aggatgaccc ctgaataagg atcgcggcgc tgggcatctt
     3301 tggtcgagta caagtcctta cggtagccgg caaagaacct gaggttcgcc ttggtccaca
     3361 agtgaatggg aacgtgagtg atttccgtcc ggaacccctt gtgagtgccg gtcttgacgc
     3421 cgtcttcggc ccagcggccc caactggctc tggagtcgat gtatatcccc gtcgagccga
     3481 gattgatgcg aggcgcaaag tactcgagtt ccggccactt cgtagcccag atattatcgt
     3541 cgttatcggt actttcttct ttgctgtagc caaagcggaa agtaccgtac tttgtgttct
     3601 tttgaatacg cgctgccggt ttgaatccaa tcttgctgcc taaggcatag ctggcataga
     3661 agctcagatc cccggactca ttgaggggta tattggcata gcctcggacg ccaaatccgt
     3721 catcgctgtt ataggtcgga cggggcagca aggtaaagag ataaggccgc gtattctgtc
     3781 gattatcgag acggccttcg tagtgtccat acgtaaacaa gcggacgtgt ttaaaccaca
     3841 gtgccgtatt ttcagacgta aacttttcgc cgggataaat gcggatatcg tcaccggtaa
     3901 tgtaatagtc cggcgtcttg gcgacggcat gaggcgtcgt aatgaggccg tgcttcacat
     3961 agccgatgtt gtcgtacatt tcggcatcgg tgccgcgaat atacgtcgtc gggccgataa
     4021 agccttcgat gttatccata ttcatgctgc tggtcttact gttataggta acatctttgc
     4081 ccgtcaaggc gttggtctcg ttgacataga cgacgggacc tgtcgtatga taaatgccgg
     4141 tattggtatt tccttcgaca taattcgcat ggacttcttc catcccctgc tgaatatcga
     4201 cattgccccg gcttttcaca tacccatcgg aattgcggac taaaaggtta tcggccgtca
     4261 tatggaccgg ctgctgaggc aagacgacat cctgagccgt cgcagcctta tctaaatcga
     4321 cgggaataat cgtcttttcc gtcaccggaa cggccatggt cttatcggta taaagttgat
     4381 ccgtatcggc tgccctgata gaaaaagtca gcggcagcaa ggcagcgacc attgccgctg
     4441 tccatgtttt attcattcgc gtcacctgct gtattcgatt gaatctggac gactgtttca
     4501 tcatcgtggc cggtattttc taaatcgaca ggcgtgaccg tccctgcttt gaggccggcc
     4561 tgcaaatcac cttcactgct gtctccgtca tgttcggcct ctgcactgtc atcaacatcg
     4621 ctgtcactga cgggattaaa cgacggaacg gttacgccgc tggccgccat atcggccgtt
     4681 tccatatcgt ccatgacacc gttttcttta aaaccgacga cttctgtatt ttccttcgtt
     4741 tcggcataca tcgtcgtgcc ggtcggaata tcgacttcat tgccgtggac aaagagacct
     4801 ccgactaagc cgacagggcc taagatgacg gcaccggcag ccgtggaacc tgaggcttct
     4861 gctcatctga aggtgacgac gttggtagtg t
//
LOCUS       DAWDXZ010000081         4877 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4605_length_4877_cov_9.083990, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000081 DAWDXZ010000000
VERSION     DAWDXZ010000081.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4605_length_4877_cov_9.083990"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    382..391
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1980..1989
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2676..2685
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3889..3898
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1185 a         1242 c          959 g         1451 t
ORIGIN      
        1 aaagaatatt ttggcacctc atctgtcggt tcaatgcgga tgccctttat atccatatgc
       61 gccttatcca aggttcgcag aataatctta ttctctgatt tttcgattac ttcgctgacc
      121 gccggctcct cttcactcga tgctaactgg agaaaggttc cccattgaac cccgttactt
      181 tccgtaaacc gggtttccgc actgcccgta ttggcatcag gaatccactc gccttcaatg
      241 gaatcaccgt cataaggcag ttgcttcaca ttccacacca attcttgatt ttcaatgatt
      301 ttatcttgaa gcccattctg ctgccagcag aaatcccatc ggctgtagtc atcgaaaaag
      361 gcttccgccg tcaaatcaag gnnnnnnnnn nggcgtatcg tcaactgccg gcgtccccaa
      421 ggggaaaaga taaccggagc ctgaattcca tatagtgccg ccagttgata gagccgggat
      481 tcccgacggc ggcaagacgg ttccaggacc aattgatatt gaaactgata ggtatccccg
      541 tctttatcgg tcacaatgcc ctcataggcc gctccctgtt cgatgacatc ctgcagtcct
      601 tcataggtcc ccttccaaaa gaactggcgg tgcccacagt ccctgacatc cgtttcttcc
      661 caatcggaac agaaaaaccc cagaccggat gtttcaacgg gatagaaatg gttttcctcc
      721 cataccccca actctgtttt gggaacgagg gccgtcgtaa tcttccagtt acggctccat
      781 ttcacccgat ccggcaccgt tttttctatc gtttctcgga ttcctttaag ttgatctcgc
      841 tcatactgac tcaatttatc aagagactga tgtattgttt ttttcatcgc ttcatcaaaa
      901 tcggcagcct caatgcgttc cattagctgc ttattgatag cctcaaaact ctccatggtc
      961 aaatcactat ccttacgaca tctgtatttt tgaacctgat aactttacat tgccggagct
     1021 gctcttaaga cttacgtcac cactcccttg aactcggaca tttccccgac tgataatcag
     1081 actttcttta ttgttttgca aagaaagggt tccatcatta tcaagggtta tatcgccttc
     1141 cgatttaatc tgcacaccgt cggtgacgtg tacttccgat tgtttgcctt tagcgtgcat
     1201 cccctgctca tctaattcaa caatcgtctg ggagccgacg gctaaattga ttttttcacc
     1261 gtccatcgta atgcggtttt tgccgacaat taactcgatt ttctcgtcat ccatgacaat
     1321 ttgattttct ccggataata aggtcaggga atctttcttc catatgattc ggcgctgaaa
     1381 atcattgccg ataaacttgt cactgacatt gcccgcttct tttcccaaag gatgattgcg
     1441 atgggccgaa tgggcataac attgtccatg taaaaaagta acttccacct gatttcccgg
     1501 atccggcaaa aagacgatac cgtcagcctt tccggtatag gcactgctgt atggaatcca
     1561 catctgcggt gaatcttggt cttcatattc cggttcattc tgcttgaagc ggacttgaat
     1621 ccgcccgagg cgttccggat cttcaacatt ggtaacgatt ccgtagagcc gcagcggtgt
     1681 ttcaaaggta aaatcaggct tttcgatatc cgctgtgtct tgatacgctt tcagttcata
     1741 ggtaaacaaa tctcggttgc ccttatactg tgccgtaagg gccgtaatga cgtatttctc
     1801 tttcagcgac gggaaggcca cggtacatcc cagttcaaga tagcgcggca gcgtcacttg
     1861 cagctgtttt tgtttttgca gtccgactcc cgtcttccaa cataaaatat cttcttcttt
     1921 aatcggtacc ggatctttat gccaggccca atccattttc agcaccacgc cctgcggccn
     1981 nnnnnnnnnt gtcctgcacc cataagggac tctgcaaggc cgctgcgcag cgccggatga
     2041 aggaaaaatt actttcccga ttttgaatga ccgtctgaac agcaggtatt tttttcagtg
     2101 attcagccac ctgcagcttc acatcgccgg atttcatatc cttggctaaa gccaatcgct
     2161 cgccatccaa tatgtcaccc atttttttgc cgctgtcctg aaagatacgg catcgttcct
     2221 gctgatccaa aaaatgagaa gaagatacgg cacagaggca gaggaccgac cgatggaacg
     2281 tctgttgtat gtcaacggtt tcaacgattc cgctgaacac gatcggtccg gttttatgca
     2341 aatcgaaaga ctggacaatc gtaatgggct ttccgacctg ctgtaagtaa aaactctctt
     2401 taccgtccgt caggtgagct gtcagcgtca agcggctgtg ctgtcccatc tcttttctta
     2461 ctgtacaagt ttcaacgacc agtccgtctt tgattccttc aatctgcatc tgtgccgcct
     2521 cctttctacc tagaatgttt caatcgattg taacgaattt tttctctgct tcaacactaa
     2581 cttgaagttt cccgcattcg attctcccca atcctcctca tagctaagga taaacatttg
     2641 cggaaagata tatgtcctca gtacagtatc agttcnnnnn nnnnntcctt cttgtgcatc
     2701 cgtaagttca acttgtcgat aaattccatc agcagtaaaa tcacggctcc aacgagatac
     2761 ctggattaca gcctctttgc tgtcatcatc aacacttccg gaaatagtca ccgtcgctcc
     2821 cacactgttc gatttctgaa aaacatgttc ttcctctgta tcaaaaagaa ccttaatacg
     2881 aatatcctga tctaaagaaa tatcattttt accacctgta atttttactt tgcacggcat
     2941 gattatccac tcctttattt attctgatct gacgaatcat cgctgatgat atcgatattg
     3001 tctaatactt cagagtcacc attgaaggta atcttcaatt gatttgaatt gggttcactc
     3061 caaccgacat cttcgccctt tttcaatacc agattgattt tgggattttc ggcaaactga
     3121 tcggctgcta acttccattt tgctgccatg tcccggatta acttagcgtc aatctcatcc
     3181 cgcttctttt tgaagatggg cacggctaag gcataaatga agtcttccat caatacgcga
     3241 taaacgggct tgtatttttg attttccatc ttcgctaaag tacgagccgt caggacatag
     3301 gtattcttcg ttttttcagc cgtttcatcg ccgcagaagg agaagccgaa catggaatcg
     3361 gtaatggcat tcagtaccga ctggctccaa gccgtcacat tttcccgatt gaacttagtc
     3421 gtcaaatgat cctgcacaac tttgtcttct aagtcgaggc gaacaaaatt catattatct
     3481 cggttagtaa tgataaaatc ccctaaaccg cggttcttta agcattgaag ctgctgactg
     3541 gcaactaaca ggccggctgc cggataggcc gcatcgatat aaatggccgg tacggtcagt
     3601 ttactgcctt ctgtatctcc ggttaccgag atgtaccgtt cacgcataag cgtaaagttg
     3661 ggataggcat agacggcatg gggataattg atgcctcttg attcaaacat gcgagccgta
     3721 tcctgaatat aggaagctgt aatctgtgaa aaagtgtttt ccggcccctg attcaaattg
     3781 aaggtcgtca tgatatgggc ttcattcaaa tccttcagca gtaccgcagc gacatctaaa
     3841 gtcaagtcat cgcctgattt agttttcttt tgttcatccg gctgaatann nnnnnnnngg
     3901 actggctgaa gtcaaagtca tcttccttcg tcaccggtgc tgattccgcc ttatccttta
     3961 cagacgggag aataccaaac cagcacttat cattgcctaa atcacggccg atataattga
     4021 tgtatttttt aaatttagca ttcaggcggc cgtcgacgaa atcagcaggc ttacaattgg
     4081 ttacgatcaa accgctttcc aaggtatccg tttcactgcc tgtcgccgtc gcatgatcaa
     4141 aaaagaccat gacacccatg agtttttcaa tgatcttcga cagttctttg atgaattcat
     4201 tcttcgcgtc tgaataaacg tcttcatatt gcttcttctg cgtttccagt acactgatag
     4261 cctgctgcac ttcatcagcc tttaaatgtt gcaaggggtt gtttacggca gtcggcgagt
     4321 acgcagccaa cactaagtcc ttgataaagg gattttcatc ctgccccgga tgatattctc
     4381 gatagtctgc ttcaatcatc tgataaatat cttcgcccac attatgctgt gatgatgcta
     4441 aaggtaaaac ttcagtagtg tccgatgagg atgccggcag aaactgcatc cccccttgcg
     4501 tatcaaaagt ccaatggccg ctggtcaact ttggaactgc ctcttcttga agctgtccgt
     4561 ccgtactcgt gccggcctgc aaaacatcta attttttcgt cacgtcatca atagccaacg
     4621 gcagtaaggc aatcacggac tgactgtact tttcagtgat ttcttttcga taggagtaca
     4681 ttttatccca atattccctg ggattttcgc cattatcccg ggcttcatga aatttctcag
     4741 tcacatattg aaaatttttc cgcagtcggg ctgcctcttt tacttctcga tcaggcgaca
     4801 gcatattcaa aagatctttg tcatcgaatt cataattcga ctgaccgctt tgcccctttt
     4861 gtttatacat gttggcc
//
LOCUS       DAWDXZ010000082         4785 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4686_length_4785_cov_10.118182, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000082 DAWDXZ010000000
VERSION     DAWDXZ010000082.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4686_length_4785_cov_10.118182"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1544..1553
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1067 a         1339 c         1224 g         1145 t
ORIGIN      
        1 tgcatcaggt ctgcaaagca ttcggcattc ccgatgacac agacgacgtc aaagcctttg
       61 acggaaaatt ggcctacgtc ctcctggccg acctctcccg cccgacaccg gatgactatg
      121 aaacgatcaa agccatggcc ccggctgaac ggcaaaaagt ttggaacgac ctcgacatca
      181 tccccatcag tgcttacaat gaagtctttg acgcctatca ccgcaccggc gtcggtacgg
      241 atggagactg gcagagcatc atgaaacagt tcctgcgctg cggacttgct ttctgcttta
      301 ccggcgtcgt cgctgccaac atcgccacag acgtcgtctt cggcatcggc catcgggcta
      361 cttcaaaagt aaatatcggc gctctgaaaa aaggctatgt caacatcgcc gttcacggcc
      421 atctcccgac cttggtcagt gaaatcgtcc gtatcggccg cacccaggaa ttcatcgacc
      481 tggccaaggc gcacggtgcc aagggcatcc aattctacgg cgtctgctgt tcctgcctgg
      541 ccgccatgta ccgctatgaa ggggttatcc ccctgtccaa tgccgtcggg gccgaactcg
      601 ttctcgggac gggtgccctc gacttgtggg ttgccgacgt acaggatgtt ttcccatcca
      661 ttatggacgt cgcccgctgc ttcaagacga ccgtcgtcac gaccagtgat tctgcacgcc
      721 tgcccggtgc cgaacactac gcctacgacc atcaccattc caacatcgaa gatacggaaa
      781 aattagccag aaaaatcgtc acccgggcca tcgaaagctt cgaagcccgc cgcgacgttc
      841 cggtcttcat tccgtcttat gaagtcacgg ccgatgtcgg cttcaatgct gaaaacattg
      901 ccgaagaatt caacggtttc ggcccctttg ccgatgccct taagagcggg cagatcaaag
      961 ggatcgtcaa catcgtcggc tgcaacaatc cccgtgtcgt ttacgaacgg gccgtcgttg
     1021 acgtggccga agaactcctg aaaaacaaca ttctcctgtt caccaacggc tgtgcttcct
     1081 tcccgctctt gaaactcggc ttctgcagta aagacggcgc cgtcaaagcc ggtgactccc
     1141 tgcagaaatt cctcggcggc aagctgccgc ccgtatggca catgggtgaa tgcgtcgaca
     1201 acacccgcgc atccgtcgtc ttaggcggta tcgcccaggc tgccggccat gacatcaagg
     1261 acatgcctta cggctttgcc agcccggaat ggtccaatga aaaaggcctc gatgcctctt
     1321 tggccttccg cctgttcggc atcaactctt atcactgcgt cgaaccgccg gtccaaggtt
     1381 cgacgaacgt cgaaaacttc ttaaagcgcg ataccaaaga aaccctcggt tcggtcatga
     1441 cggtcaacgt cgatcccaag gcactggcaa aacagattgt cgccgacatc gaagaaaaac
     1501 gccgcaaatt aggctgggac taatcccttt ctgtcgctat taannnnnnn nnngagagag
     1561 gatattttga tgttgtttta tacgcttaca tatcgcggct ggctacgata tcgacgccga
     1621 ggcctaagat atctttgacg atgacgtcgc ccgtctttac cggagctttg acgtgaacat
     1681 cgcgcagagc cagggcgcag tcgaggattt tccccttggg cagtgtcgtc ttggatacga
     1741 ccggcaagag cggcagttcg ccgccttcga tgctgaccgt cgacgtcagc atgcgctggg
     1801 gattggtcaa ttcctggtgg gcatacgttt cgccgcgagg acaggtgttg ccggtgacgt
     1861 tcgtcacttt accgtcttcc agggtaaccg taagcaagca gcccataggg cagttgatgc
     1921 agtttaattg tttcgtttct acgctcatat tatgcttcct cctttacgga aacagtccaa
     1981 tccgcatgat gacctttcgc cttggcagcc ggtacgttga cggcaatcat ttcactcggt
     2041 ttggcaacgg ggaggacttt cgtcagcagc gtatcgtcac cgcttttcac agtaaggcga
     2101 acttttttca tcggtttgcg gacacgcatg aagagtcgaa tcgtttcccc ttcagccgta
     2161 gcgcggacat gctggggaac gcaggttctg acttcgtcaa cggcttcaac ggcgatgtcg
     2221 gcaacgtctt ccggcagctg ttcctgtgcc ttcagggcgg catacttacc ggcgatttcc
     2281 gcttcttccg agacgaagtc gactaagtcg tggacgtgga cgacattgcc ggcagcaaag
     2341 ataccgggaa tgctcgtttc acgatgctgg tcaacgatcg gtccgttggt caagggatgc
     2401 atggcaattc ccaggccgcg ggacaattca ttttccggaa tgaggccgac ggacaacagc
     2461 agggtatcgc aatcgatatc gaattccgtc cccggaatcg gtttcatatg atcgtcgact
     2521 ttggctgccg taataccgct gacccggtct ttcccgtgaa tagccgtaat cgtatgcgac
     2581 agatacaggg gaatatcata gtcatagagg cattgaacca tgtttcgagt aagaccgttg
     2641 gagaaggggc agatttccaa aacggcctgc actttacacc cttccaaggt catacgacga
     2701 gccatgatca agccgatatc accgctgccg aggatgacga tttttttgcc cggcaagtag
     2761 ccttccatat tgaccatacg ctgggcagca ccggctgtga aaacgccggc cggacgatag
     2821 cccgggatgc ggatagcacc gcgcgtccgt tcacggcagc ccatagtaag gatgatggtc
     2881 ttgcccttta ctttcatcag gccgtgttcc gggctgacgg cggtaacggt tttatcctga
     2941 ccgatgttta agaccatcgt atctacgagg attttgattt ccggcttatc tttgaccaaa
     3001 tcataacagc gctgggcata cccgggaccg gtcaattctt ctttaaaatg atggagaccg
     3061 aagccattgt gaatacactg ctgaaggatg ccgccggctt cacggtcacg ttcgagaacg
     3121 aggatttctt tcgcgccgtt ctgccaggcg ctatatgcag cagacaggcc tgccgggccg
     3181 ccgccaacga tgataatatc ataaagttgt tcacccatta tgccttacct ccgctttctt
     3241 tattatagaa catataagaa ttacgcattt ctttccggac ttccgtaacc gggatgttca
     3301 attcacgggc caaaatctga gttacacgag gtccgcagaa accgccttgg cagcggccca
     3361 tgccggcacg agtacggcgt ttgacaccgt ccacagtgcg ggccccgacc ggacgatgga
     3421 tggcatcgac gatttcgcct tccgtaatcg tttcacagcg acagatgacg cggccgtaac
     3481 gaggatcttt agcgataagg gctttttggt ccgcatcgag catcatgcgg aagcagggct
     3541 gggccggacg gcctttttta taatcggact tcgccgaagc acccgtttca gatacgatga
     3601 ggtccaccag ctttttggca atggccggcg ccgacgtaag gcccggcgac tggatgccgg
     3661 cagcatggag cagtcccgtc gtcttttccg aaggtccgac gataaagtca cccgtattgg
     3721 aaacggcgcg gacaccggaa aattcggtaa tcgtagcacc gaccgggata ttggggacta
     3781 agcggcgggc tttgtccaga atttcgtcca tgccgggaat ggtaacggct gtatcatcct
     3841 tgtcatcttc cggcagatcc tgggcgttag gccctacgaa tacgttgccg tgcgtcgtag
     3901 cacagaccag gatgcccttg cccatcttag tcggcgtcgg gaaaataggc gaataaacca
     3961 aatctttctg agccgtcttg tcgaagagaa tatattcgcc gcgacgcggt ttaatcgtaa
     4021 agctgtcgtc accggccaga cggctgatat cgtcggcatg aacgccggca gcattgatga
     4081 cgtacttcgt ttcaatcgtg cctttatccg tttcaacggc ctttacggca ccgttttcta
     4141 cgacgatgtc cgtaacctta cagtcacgga tgacttcggc accgttgatg acggcatttt
     4201 ccgcaaaggc caacgccaag ccgaagggcc agcagatgcc ggctgtcggg gcaaagagag
     4261 ccgctttgat gtctttactg acattcggtt cttcttcgcg catttcatcg ccgctgacga
     4321 ttttcaaacc gggaacgccg ttagcctggc cccgggcgag gagttcttta aggacttcca
     4381 tctgttcatc gtcgagagct gctacatatg acccggacca acgaatttcc aaatctaatt
     4441 catcctttaa ttcatggtac atcgcattgc cttcaacatt catcaaagct ttcatgctgc
     4501 ccgtaggagc atcgaaccct gcgtgaagaa tggcactatt gccttttgtc gtaccggctg
     4561 ctaattccgg ttcctgttca accagaacgg cgcgaagatt atacttggat aattcgtgca
     4621 aaatggctgt gccggtaata ccgccgccga ttacgacgac atctgcttgt tttgtcatat
     4681 tcacaacctc tttctctgta aatgccttgg agtactatct ttgatgccct tattatataa
     4741 gttcgtgaaa ataattgcaa gtctattttt acattttttc aattt
//
LOCUS       DAWDXZ010000083         4783 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4688_length_4783_cov_9.674492, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000083 DAWDXZ010000000
VERSION     DAWDXZ010000083.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4688_length_4783_cov_9.674492"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2514..2523
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1076 a         1268 c         1285 g         1144 t
ORIGIN      
        1 gcatatgatt cctaactgcg tcgggccgat catcgttacg ctcaccttat cgattccgga
       61 agccatcttt acggaagcct ttttaagctt tatcggtctg ggagtatcgg caccgatggc
      121 gagttggggc gtcctggctt cagaaggtat taacagcatg cgctcctttc cctttcagct
      181 ggcctttccg gccttggccc tgtgcattac catgctgggc ttcatgttct taggcgatgg
      241 cctgcgcgat gccttggatc cgcagcatca aaaggagggg agccgatgaa tccgttactc
      301 gaagtgcgtc atctgtctat ttcattccac acgtaccgag gccttttaga agccgttcac
      361 gacgtatcga ttacggtccg gcccggtgaa accgtcggca ttgtcggcga atcgggatgc
      421 ggcaagtcgg ttacgtccca ggcggttatg aaattattgc cccagggagc tgcggaatac
      481 cggaacggcg agattttgtg ggagggacgc gatctcctgc ccttgtcaga atcggagatg
      541 aatggccttc gcggccgcga tatggctatg atttttcagg atcccatgac atccctcaat
      601 ccggttctca ccattggggc ccagctttgt gaagggcttg ccctctatca gaaactgtcc
      661 aagcaggagc agcggacaaa ggctgtagaa ctgttaaaag ccgtcggtct gacttcaccg
      721 gaaaagcggc tcgaccaata cccccatcaa ttatcgggcg gtatgcgtca gcgctgtctc
      781 attgccatgg cactatcttg ttcgccgaaa ctcctctttg ccgatgagcc gacgacggct
      841 ctcgacgtta cgacagaagc acaggtcttg gacgtcttaa agaaaatgca ggcagaactc
      901 ggtatggccg tcgtcctcat ttcccataat ctaggcgtca ttgcccagat gtgccgccgc
      961 atttacgtca tgtatgccgg taccgtcgtt gaagaaggga cggcagagga tattttttac
     1021 cgaccggccc atccttatac cagggggctg ttggcctctc ttccggaccc aacggatcgg
     1081 gataaggtac tgaccggcat tcccggccag gccccggatt tattccatat gccaaaaggc
     1141 tgccggttct atcggcgctg ccctcgggct atgaaagtct gtatggaagc agaaccgccg
     1201 cgggaagaag ccggtcccgg tcatcactat acctgctggc tggcagcggc agaaaggagg
     1261 atgggcgatg gcactcattg aagctaaagg ccttaccaaa caatttgtga ttgaccgcaa
     1321 ttggttaggt cgtccgaaaa aagtgttgac cgctgtcaat cacgtatcct ttacgattga
     1381 gccgggcgaa acggttggct tggttggcga atcgggctgc ggcaagtcga cctttgcccg
     1441 caccttattg ggactgtatc ctaaaaccga tggggatatt ttttataaag gccgtaatat
     1501 cggcgagcct gaaatcggga aggactatcg acgccaggtg cagatgatct ttcaagaccc
     1561 gtatgcctcc ttggacccgc gaatgaccgt cgaagatatc attagcgaac ctcttcgcaa
     1621 ctatgcgctc tgttccgacg atagagaacg gcgtcaacgg gtggccgatt tattgaccca
     1681 agtcgatttg agaccggaaa cggcccagcg ctatccccac gaattcagcg gcggccagcg
     1741 tcaacgcatc ggaatcgccc gagcccttgc cgtcgatccg gaatgcatct tttgtgatga
     1801 accgatttca gctcttgacg tatcggtcca ggttcaaatc gtaaacatgt taaagcgcct
     1861 ccaggaagaa cggcagttat cgtatctctt tattgcccat gacttgagta tggtccatca
     1921 tttgagtcgt cgcatcggcg tcatgtatct gggaaatctc gtcgaattag gtccgagcga
     1981 cgccgtcttt cataggcctc tccatcccta cacacagatg ctcattgcct ctgtgccgcg
     2041 gccggatccg cattatcaga gaacggccgt tgtttcgggt gaagttccca gccccctcga
     2101 tgtaccgtcg ggctgcgtct ttcatccgcg ttgtcctgat tgtgatgaac ggtgccgccg
     2161 tgaggtgcct gccctttgct ccgtcgggga agggcgcctg gttgcctgtc accatgtcaa
     2221 aggagggacg gactgatgaa aaaatatatc cttttattcg tggccgtcct catggccgct
     2281 tttctcagtg cctgcggcgt agaacaaccg gatacggtga gctacgtctt agaagcggag
     2341 ccgtctcgcc tcgatccggc tatgacgacg gccttggctg aaagcaatac agaattacag
     2401 atttttgaag gcctgacccg tcttgtcgat gatgttcctc agccggcctt ggctaagagc
     2461 tgggatattt cggccgatgg cagggtttat acctttcacc tccgcgacgg aatnnnnnnn
     2521 nnngagcgac ggtacgccta ttacggccgg cgatatcgaa tactcctgga agcgcgtcgt
     2581 caatcccgac gttgcttcgg aaaatgccta tatgctcttt tgcatcgata aggccgaaga
     2641 ttactttaag aagaaggcct cttctgatga agtcggtgtc aaggctctcg atgataaaac
     2701 cttggaagtt cgtttaaaag agccgacatc ctactttttg aatctgacag cttttcactg
     2761 ctattatccc gtaccgcgga agctcgtcga agcaaagccc gatacctggg ccgccgatgc
     2821 cgagggaatg gtttccagcg ggccttttaa gattgtcagt tggaatcatt ccagtgaaat
     2881 taaattggtt aaaaatgaac gatattggga tgccgatgcg gtgaagctca gccacatcga
     2941 tcttcccatc agcgattctc aggcgacgag attgactttg gtagaaagca atcaagccaa
     3001 tatgaccgtt gagccgccgc cggccgacca agaacggctg gaaaaaatgg ggctctataa
     3061 aattgctccg tatttaggcg cctattatta cgtctttaac gtccagcaaa aaccctttga
     3121 cgacgtccgc gtccgcaagg cctttgccct ctcgattcag cgccaaggcc ttatcgacca
     3181 tattatccgg gctgaaaagg aaccggccta tgcctgggtt ccgccgggtc tgaaagataa
     3241 ggccacaggc cgtgattttc gggaagaagg gggcaatctc atcagtgaag atccggaaag
     3301 ggcacgaaat ctcctgcgtg aagccggtta tgatgaaaac catcctcttc cgccggtgac
     3361 catcctgttc aacacgaacg agatgcacaa ggccgtggcg gaagcaatcc aggccatgtg
     3421 gaaggataat ctcggtgtgt ccgtatcgct catgaatcag gaatcgaagg tattcatggc
     3481 ctctcgggcg cagggggact accaggtcgc cagggcttcg tggattgccg attatgccga
     3541 tcccatgacc ttcctcgacg tctttgcgga cgaagaaaac gatgcccagt atcacaatac
     3601 ggcctataac gaactgattc gccaggctaa ggcgacgaac gatgaagccc ggcgcatggc
     3661 ctatatgcat gaagccgaac agatgctctt tgatgactgc gtcatcatcc caatctacta
     3721 tacgacccag ccttacgtag cccagcctta tattaaagga taccattggt cacaactggg
     3781 attggtagat tttaaaaatg cctatatcga tgaaggagaa aggacgtaac cctatggatt
     3841 atcgttgtgc cgcccctttg aatccgggcg atacgattgc cgtcgtggcc ccgtctgcgg
     3901 cccttgccga agatgatgcg gcccaaggca ttgccttttt aaaagaactt ggatatcacg
     3961 taacggtggg acaatccgta tcggctcacg acggctattt ggccggcagc gatgaactgc
     4021 gggctgctga catcaacacc gcttttgccg atgaccatgt aaaggctatt atgtgtcttc
     4081 gcggcggtta cggggcaacg cggatcttac cgcttctcga ttatgagtta atcgccgccc
     4141 acccgaagat tttcatcggc tttagcgata ttacggcgct ccataccgtc tttctgcagc
     4201 gctgccattt tcggcccatc cacggaacga tggtcatgtc cctggcccgt aaggcttctg
     4261 actacacacg ggaagaattg cgctggggat tggcccatcc ttatgctgca cgcacattgc
     4321 cttcggcgcc ggcgatgcat cccgagaccc tcgttcccgg gacagcgacg ggacctctct
     4381 taggcggcaa cttgatgctg ctgtcggtta ccatggggac gccttatgcg ttacaatcgg
     4441 aagggggcat tctctgtttg gaagaagtgg gagaagatgc ctattctctc gacagaatgc
     4501 tctgtcatat cgagcaagcc ggcctcattg acggcgtgaa aggcattgcc tttggcgaat
     4561 tttaccgatg caatcctacg gaaaggagca cctacgaatg gactgtcgac gaagtcattc
     4621 gtgcttatgc gaaaaaatgg ggaaagcccg ccgtcaaggg cctgtccttt ggacacggtg
     4681 ccgataacgc ttggcttccc ttaggtcaga cggtgcgcct tgaagccgaa acggatcggg
     4741 cggaactcgt atttttaccc taactgtagg aggaatcctg atg
//
LOCUS       DAWDXZ010000084         4776 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4697_length_4776_cov_6.846431, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000084 DAWDXZ010000000
VERSION     DAWDXZ010000084.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4697_length_4776_cov_6.846431"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    777..786
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3097..3106
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1113 a         1122 c         1396 g         1125 t
ORIGIN      
        1 ggtgacggca gttcctgctg ctaaggggga atgatgatga ccttatataa tcctttagag
       61 gttgtccgta cgaaatacct ctttttgacc gggaagggag gcgtcggcaa gacatccgtt
      121 gcctgtgcga cggcagttgc cttagctgat gccgggcgta aggtgctgct catcagtacc
      181 gatccggcgt cgaatctgca ggacgtcttt tccatggatc tgagtgatga ggctacgccc
      241 attccggaag cggccaatct gtcggtcgcc aacttagacc ccattcaggc ggcagctgcc
      301 tatcgggaga gcgtcgtcgg tccgtatcgc ggtctcttac cggaagcggc cctgcgcaat
      361 atggaagagc agttatccgg ctcttgtacc gtcgaaattg cggccttcaa tgaatttact
      421 cgcttcctga ccgatggcga gatcagtaaa aattatgatc acatcatctt cgatacggcg
      481 ccgaccggcc atacactgcg gatgctgcag ctgccgtcgg cgtggagcca attcattcaa
      541 aagagcaagc acggcgcgtc ctgcctgggg cagctggccg gcttggagtc aaaaaaagaa
      601 gtatataaaa aagccgtgga aaccttggct gacgagacgg cgacgacgat gctcctcgtg
      661 gccagaccgg acctgctgcc tctaaaagaa gccggtcgag cctcggcaga attggcggcc
      721 ctgggcattt caaatcaagt tatcgtcatc aacggtaaac ttacggcttg ggacgannnn
      781 nnnnnncggt ctcgaccatg tacgggccct gctcaaggga gaaatggccg aagagaaagg
      841 gaacgctgac gagatgaaaa aggtcagctc tctttcgtcg gtcattgatg acttagaaca
      901 aaagggtcgc aagatcatct ttaccatggg caagggcggc gtgggtaaga cgaccgttgc
      961 ggcggccatt gccatggaac tggctcggcg cggccaccgc gtccatctga cgacgaccga
     1021 tccggcagcc catgtaggcg acatcgtcga acagacggca ggcctgacca taagccgcat
     1081 cgatgaaggc gcggccttac agcagtatcg gaaggccgtg ttggacgaag ccaaagccaa
     1141 cggtttagga accgctgatt tggcctacat cgaagaagac ttgcgctcgc cttgtaccca
     1201 ggaaatcgcc gtcttccatg cctttgccga catcgtcgac cgaggcggtg accaaatcgt
     1261 cgtcatcgat acggcgccga ccggtcacac cctgctgctc ttggaatcga cggaaaacta
     1321 cgataaagaa atccgccgga ctcgcggcgt gacgccgccg tcggtacagc acttactgcc
     1381 gcgccttaaa agtgacgata cggaagtggt catcgtcacc ctgccggaag cgacgccggt
     1441 ctacgaagcg cagcgacttg aagatgactt gaaacggacg gaacttcact gccattggtg
     1501 ggtcattaac aaggcttttt gccgggccgg gacgacgagt cctttgctcc gggctaaagc
     1561 caatcaggaa attccctgga tcaataccgt tcatgaccat acgaagggcc atgcggcctt
     1621 gattccttgg aaatcggctg aagtgcgcgg ctctatgtta gaaaccttat aagcaagccg
     1681 gcatcgatac ttttgaagga ttggtgataa tcccgatgct ctgcagagac ggtcggcgct
     1741 ttgaccgaag gcagggctgc tgaccttggt ccgcattgcc ctggcgacca agtagatttg
     1801 attttgacat gaaaaaggac tctatgaaat ttcatagagt ctttttttcg tatgctgtta
     1861 tatgggaagg aggagttact tgccttcgta atagtagcga gtccacatga cgacttccga
     1921 gagagacggg tgaggggtca tggtttcgct gaaggtgtag accgatttcg gcgtcagggt
     1981 ccgcgtcaag ggcagggccc gcagtttctt ggccgtgtcc gaagcgatgt cttcgtggac
     2041 gggctgaatg gtgtgttcgc cgctcatgag gttgttgatg aaaggctgaa tgaggatcga
     2101 tgcttcaggg ccgatgatgt gggcgccgag gatgaatttc gtctttttgt cgacgactaa
     2161 tttgatgaaa ccgtcgtcgt cttgtcccgg ttcaaagccc atggcgtagc ccttggccga
     2221 agccgagtag tggttaatgg ccgtttcgac gtcgtagccc ttctttttgg cattttcttc
     2281 gctcaggccg acttgagcca cttcgggata ggtgtaggtg acggccggga tgatgtcgta
     2341 gctggcccag cgccagtttt ccggttcatt gcctgagaag aggttgtggg caatgatttc
     2401 ggcttcgtaa ttggctttgt ggcggaaggg ggccatgccg ttgatgtcgc cgagggccca
     2461 gatgccgtca gccgtcgttt ccaggaactc attggtttcg atgtagcccc gtttatcgag
     2521 gcggacgtcg gtattttcga ggtggaggag atccgtcgtc ggcttgatac ccggggcgac
     2581 gagtatttct tcggcagcca cttcggcttc ttcaccgctg tcgatgtcgc ggatcttgag
     2641 gactttgagg ccgtcgcgga cggtgacgct taccgtgtcc tgctttaagc ggacgtccat
     2701 gccgtagcgg cggaactgtt tgaggatgaa ggcagaaatg gcttcgtctt ctttcggcag
     2761 caggcgggcg ttgtgctgga cgatggtgat cttcgtgccg gctgcgtcaa agacgtgggc
     2821 aaattcacag ccgatggggc cgccgccgat gatgatcagg ctcttataag gcgtcttggg
     2881 gaaggattca ccgaagaagg attccgacgt cagatagccg acgtcttcga ggccctcgat
     2941 gcgggggatg ttggtccggc caccggtggc gatgaaaatc ttgtcgccct ggatttttgc
     3001 gtccgtgccg tcatttaagg tgacgtccaa ggtgtggttg tcgacgaagg ctgccgtgcc
     3061 gttgtaagtg tcgacgttgg ggaacttgtc gtagtannnn nnnnnnccct ggatggattt
     3121 actttcatcg attttttccc agagtctttt ggctaaaata tcccagtcga ggcgggcatt
     3181 gtcggcaaag acgccgatgc ggccgccttc gcggatttca cggatgcggc tggctgccgt
     3241 gaccatgacc ttagtcggga tgcagccgcg gttcaggcag gtgcctccga aactgccccg
     3301 ttcgataatg gcaacggaca acccttgggt aatggcggcg tcggcaatga tgttggccga
     3361 cccggtccca atgaggatga catcatattt tttcatgagc aagactcctt tacttttcag
     3421 cttcataatt catatttaaa tacaaccttt ttcgtatagt ataagcgaaa ggggacggtt
     3481 tgacaagtgc cgaagcccct tgtttcgtca tgatgggaaa ggagaaaaga tggtctgaaa
     3541 atgagtgatt ttatgggata aaaccaatgg ctatgacaca atctgtccat actatatggt
     3601 agactataat cagcgacaag actatcgtgt tgcaaagaat tcgtgaattt gtaatatttg
     3661 tcgaaaataa caaaggcctt tcttgtactc tttttttatt catggtaaca taatactatg
     3721 tatgacgata tggcatagtt gttgtgactt atgagttcat gacatcatta tatagtggag
     3781 gtatggttat gggattgatt ttaaatcgag atgacttggt gaaactattg caggaagatg
     3841 aagaaatccg tgatttgatt cggcagattg ccctagggca gcccgaagaa ggggaagctc
     3901 acgaagtccc ggaagcaccg gaagttccgg catcattaaa ggttttggaa ccactgaagg
     3961 aagcggtcta tattgcaaag aaagaggaac tcagttctaa gaagcaggaa cttcgacaaa
     4021 aattggaaga ggctcgaaaa gatagagacc tttggaaaca agcggcacag caggccgcgg
     4081 cggaaaagga agccgcagtt cttgagcgag accgggcaat caatgaacgg gacgccgccg
     4141 tgtatgaacg aaatcaggct gttcaggaac gggacgctat ggcagaccaa ttgaaacagg
     4201 ctgtccgcga aaaagaacaa gcctttcgtg ctatggaaga atctgaccgg ctgcggaatc
     4261 aagccgtccg cgcaatggag gaatcgaatc ataaggtaga agaagcgcgg cgtatcgttt
     4321 ctaaacttca ggctgaaatc acctttttgc aggaacgact gggtgaagct aagggaacaa
     4381 tcagctccct tacggcagat ttagatcata cccgggaaga acgagaagat ttaaaagatg
     4441 atttacagca ggctgaacaa gaattggctc aacgctttgt ccaaggttgg cagctctatg
     4501 aagcctacca agacgtcgat gactattata aagacctctt ggacggcgtg ttggctcgcc
     4561 ccggttttga agccttcatc gtaggcctat ctcaggaccg gtctttgcgg accctgtggg
     4621 atgtgacccg ggaagcagtc atgaagagtc gtgacgaggc tgccgggaaa atcctttgga
     4681 atctgttctg ctacagtttg gacttggtca atcggagccg gtcggaaaat ctctacgccg
     4741 tcgatgtcgt agaggtcggt gttccttgcg atattg
//
LOCUS       DAWDXZ010000085         4747 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4720_length_4747_cov_5.759591, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000085 DAWDXZ010000000
VERSION     DAWDXZ010000085.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4720_length_4747_cov_5.759591"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1016..1025
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1343..1352
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3761..3770
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1290 a         1321 c         1017 g         1089 t
ORIGIN      
        1 gcggacaggc tgcccacttc atagcgggac ggaaaatgct ccagggtgta ccctttttcc
       61 gccaacttcc gttctaagat ccgccggaca ccggcatggc gttcgccgat gatgagacga
      121 tggtgcataa gatccgacaa ggtcatttcg acgggcgtat cgtacccgtc cgaacagacg
      181 gcaacgatgg gttccgtaga aaataaaagc gaatcgtaat gcccttgatc aaacgaactt
      241 tcgacaatgg caaaatcaat gtttccctta tctaaggact ggagcagttt atcggcatcg
      301 gcaatttcca tatgaagatt catgtctcca ttctgcttga gaatgagtga caatttatcg
      361 agaattccca tgccggctgt tggcgtcgcg ccaaaagaca aggagcggtt atgctgctgc
      421 aagtcatgca gatgctcttt taaaagcgta tcatcatgac taatagccgt cgcagcattc
      481 cagagaatgt gcccggcttg tgtcaactgc agatgtttcc ccttgtaact aaaaagtttg
      541 gccccgtatt cgcgttctaa atatcgaata tgctgagaaa cggcaggctg cgtaatatgg
      601 agttcagcgg cagcccgggt ataattcatg tgacggcaaa cgcacaaaaa tgtatggata
      661 cgaaaatcta acatggcacc ctttcctttc tttgcgtttt ccataagata attttatgat
      721 atttcagtcg ttttatcaag aacaaagtgg gcaatttcag tatactgatg ggacgacatt
      781 gttcccaatg gaacgtattc tcaacaaggg cctgccaaat caccgcttcc ctctactctt
      841 gtttttttca tatccaaacg atccttagcc gtatccctat taatagatgt aatatatatt
      901 aactataatc gataacaaca aaatatatct tattaataat gattattgta taatagaaat
      961 aaaaaaacct gttatttcaa ggaaaggtac ccgatatgac aacagaacaa tatatnnnnn
     1021 nnnnnatgca gcttctggcc gtacagttaa acggccaggc tctccaatat attaccaatc
     1081 cttcgcctgc cgtccaggaa gcagccattg ccaacacggc ccgggcgatc cagtttatcg
     1141 acaatccctc agaagacatc aaacttcaag ccgtacgcgc cggctggaac aatttaagct
     1201 acctcgaaaa tccgtcgcag gaactcatcg acgccgcctt agaccagtcc ggctgggcca
     1261 ttaaatacat caaagatgcc ggccacgatt tgcagctccg cgccgtcaac aaaaactacg
     1321 acgccgtcaa atacatcaaa tannnnnnnn nngacctggc catcgtcgaa gatgcccaag
     1381 ccctgcggct catccgcaat ttgggaaaag aagattttct ccactacctg ggcctcaacg
     1441 tcctggtcag taaatacatc cccaaagata tcagcctcac gccggatgac tggcgcgata
     1501 ccctcaaggc tatcctcgcc caagaagacg tatccgaaga atacgtccgc aacttcatta
     1561 cctgcagcgc cgtcgactta tcgcctgtcg acaaggtact cctcgtcgat gactatggca
     1621 gcccaaaggc ccgacgcatt actgtagatg aaaaattgac catgaaatag gagtaacgac
     1681 atgaacttta cagataccta taaaagcgta gaactcgttc tcactgccgg tgaagttccc
     1741 cttctcgtcg gtgaaacggg tatcggcaag acctcccttg cccgcgaact ggcccgtcgt
     1801 cacgactgga gcctcgtcgg aatcgacgga aacttattaa aagaagggga aatcggcggc
     1861 cttccgaccg tcgaaaatgg aacgacggtc tacgccgtcc atcataaact tcgtaccatc
     1921 gacgaagaaa tcgctaaggg caaaacggtc ctcttgttca tcgatgaaat caatcgctgc
     1981 gaccacgccg tccagcaaga attgatgaac ctcatcttga accgcgaaat caacggatac
     2041 gccctgtcgc cgtcggtcca catcatcgcc gccatgaacc cctccgatgc ctacgactat
     2101 gaagtcgtcg aaatggacgc cgcccaagag aaccgcttcg tttggctgta catggagccc
     2161 gattacctgc agtggctcga ttgggcctcg tcggccggca ttgaagaaaa ggtcatggaa
     2221 ttcatttcca cctatccgga gtacctcgat aagaccaacg aagacgatat caacgccacc
     2281 cctcgcagct atgaacgaat ttcaaagctc tacaccatct ataaaaatga ttccggcagc
     2341 attccaaaat ccgtcttcca caacgtcatc cgcggcaacg tcggaaaact catcgccgaa
     2401 gaattcatcc aattcgtcaa ctccgatgcc tctcccctca tcacctataa tgacgtattc
     2461 agcgacgaaa ccctggctcc ggaattgagc ggccgcgttg ccggcgaaag ccatacccgc
     2521 ctctacctta cggccaaaaa cgtcctaaaa caattagact accacctttc ccaagacagc
     2581 gacaatcagg ccttattatc gcgcttcgtc gccttccttt ccctctatcc cgtcgatctt
     2641 cgtttaggtg tcatgaaaga catgaaagcc gccaacccca aagtctatgc ctgcgctttg
     2701 gataacgccg acttcatcga cagttacttt gcggcctttc gctccctgcg gtaaaacgga
     2761 tgaaacagtc catttccgtt gaagccactc tcctctacca gcaggcatcg ataatcgccg
     2821 acaatattct ccaagattcg gtccggcccg atccggaagc ggagataccg gctgattttc
     2881 aaaatgaatt tttcgccttc ctgggccgca tcaacggcca cctcatggaa gacaaggata
     2941 attttttcgg atactttctc ttccagatga caaagagtat ccgcttcgat ttggcgtccc
     3001 cgacaggtgt caactttaag ggcacccgct atcaaatcta cttcaatccc ctcctgttcc
     3061 tgactctgtc gccggaacag atggaaagca ccatcaagca cgaaatcctt cacatcgtct
     3121 ccctgcacct catccgagcc aaggaactgc gagaaaacta cagcaagcaa gccatcaccc
     3181 tggccatgga cgtcgtcgtc aatacctacc tcgaccatct gccgcctttt tcgacgacct
     3241 tggaatgggt caacaccaat ttttccctcc tcctaaagcc cttcgaaagt ctggaatatt
     3301 acgtcgataa gattcagacc gccctggaca tacggacaga gaacaaaaat ctgccggaac
     3361 cggaaagtga tgctgacgaa accgtcgccg tctcctataa ccccattacg acccacgaac
     3421 tttgggatga aagcgatccc gtcgacgatg aaaccttgaa aaagttcacg gaaaaatacg
     3481 tcgatgcctc gtcaaagggc gacctctcca actatttaga gagcatgata aaggctttaa
     3541 aagacaacaa agacagcctt ccctggcact ggtacctgaa aaaactgatc ggttcggtta
     3601 ccagcagttg gaaacggacg cccatgcgac gcaaccgccg ccagccggac cgcctcgatc
     3661 tgccgggcaa gctgcgctct catacggcaa aaatcctcgt cggccttgac atcagcggca
     3721 gcatcaccga cgccgaattc cgccaggcca tcggtgaagt nnnnnnnnnn ttcaccggtc
     3781 ttcgactatg ccaacaccca aaacatcgac ctcttggtct acttcacgga cggcaaagga
     3841 gaagaaaaac ttcaaacatc accgcgaggc tataaagtcc tctgggtcct ctcaggccac
     3901 ggtgaaaccc tctccttaaa gaacccctat ggccttgtaa aacgcttgtc caagctccct
     3961 gactacgacc ccagcctcga cttcgacgac gtccgaggcg gaggtttctc catggccaac
     4021 caagaacaca cgagtaagct ctaatatcca cattacaaag aaagaccgca cgcgtgggtt
     4081 gcgtgcggtc tttttatatg ccaaggttag aacaattttt catagaggaa gcgaatatcg
     4141 tcgagagaca attctttgca gttgttgacc aagagtcggg tctgctggct tccggccttg
     4201 accagaaaat cgaggtcctc ttttttaaca ccgaattctt ccaatttagt cggaatctga
     4261 actttttcta cgacatcttt gatttcggaa atgatatagt ccgctttagc atctgccgtt
     4321 tcagcgtatt tttccggata gaccgcatcg caaagggcag ccagtcgatc cgtgcaggca
     4381 tccttattga attccatgac atgggtaaag aggatggcat tggaaacacc atgggcaatg
     4441 tgatatttcc cgcccaaggg gtaggacaag gcatgaacag ccgtcgtccc cgagccggta
     4501 atggccacgc cgccataaaa ggcaccaata agcatttccg ttttggcgtc catgtcagat
     4561 ccatctacaa aggcttcccg gatgttgtgg aaaattttct tggccccttc aatggcatag
     4621 gtatcgctca aaatcgtcgc tttcttcgat gtgaagcatt ctacgacatg ggctaaggcg
     4681 tcgacaccgg tagctgccaa aaccttcggc ggcagatgac ggatcatttc ggcatctaaa
     4741 atgacat
//
LOCUS       DAWDXZ010000086         4725 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4738_length_4725_cov_8.729336, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000086 DAWDXZ010000000
VERSION     DAWDXZ010000086.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4738_length_4725_cov_8.729336"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1180 a         1129 c         1215 g         1201 t
ORIGIN      
        1 gttttggagt cttgcaaagg cgaggccttt tggggggagc atccgttccg gcagacgctc
       61 tgacagggcc tgttcaaggg gctggcggat gatggattcc cggttcatga tgccgccgcc
      121 gaggatgatt cgttcgggat tgaagagact gatgatattg gtgaggccgt cggccagatt
      181 ctgacagaag atatgaaaac agcgaagggc ctgagcatcg tcgctttcaa tcagttggaa
      241 ggcccggtag ccgtccattt ctttgggagg aatgcctttt ttgctggcga tttcacggca
      301 gagaaaggac gccgaggcga cgtcgtggaa ccggcctgcc gggaggctca tataggcgat
      361 ttctccggca gcatgagtgg cgccgtggag tacccgacct tcttcgatga gggctccccc
      421 gatgctggtg ccgacggtta cggcaaagaa agacgagacg tcctttccgg ctccgagcca
      481 atattcgccg agggcggcac aattggtatc gttttcgacg ctgacaggga gtttaaattc
      541 ttcctcgaac atcgttttcc aaggcgttcc tgtatagccg gggatggaga gggcgtattc
      601 taccgtgccg gattcggtgt ttacaatgcc tgccgtcgac agggcgatgc cggcaagcga
      661 tacttctttc tgctgctgtc ggacaaggtt tcggaccgca gcgacgatgg aaggcccgtc
      721 tccggagggg atgagacctc ttttgtggaa gcggccctct tcatcgatga ggccgaattt
      781 gattgccgtt ccgccgacat cgaagcaact gtacatcatt atagttcaca tcctttttac
      841 gagagaaagt taacttatac aaatattata catggatttg acaatttcta tgtaaaaggc
      901 ccctatactg ttcttatcaa tgtagtcttt taggaggtca tgatatgaaa cgacaatggt
      961 taaaaagagc aattctttct atggtagctt gtggttccct gttgggcgtc gtcccgggcg
     1021 tacaggcagc cgatgtccgg attttaccgg tagatacggc taaattcttg gcaggtgcta
     1081 aattcgactt tgatgttgaa gtttctaacg cgaaagactt gaagaacgtc gatattacga
     1141 ttaatggtca gaatgccaat caatttttcg gacagaaagt agaaggaaaa gatttgggca
     1201 acggtgttgt ttcttatcgt gtcaaccagg taaatttccc gaagaccggt aaatatacgg
     1261 tccgtgtttc ggctaccgat gctgacggca ccaattctgc cgatgcacgc tatacggttg
     1321 ttcaggaaaa agctcagaaa caggctaaaa acgtcatcct cttcgtcggt gacggcatgt
     1381 ccctgcaggc tagagaaatg gctcgtatca tttccaaagg cctgaccaac gggaaataca
     1441 acgatctctt ggctatggaa aaaatgcccc ataacgcact gatcacgacc agcggatacg
     1501 attccttgac gacggacagc gctaactcgg catcggctta tgctacgggt cataaatcgg
     1561 tcgtcaacgc catgggcgtt tatgaagact cgacaaagga tcctatggat gatccccgcg
     1621 ttgaaaacgt tgccgaaatc ctgaagcgta cgcgcggcat gtccatcggt gtcatcactc
     1681 aggctgaatc tacagatgct acgccggccg ctatgattgg tcatacgcgc cgtcgtgcta
     1741 accaggattt attagcttct cagtacttgg aagactatca tcgtccggac gtcatcatgg
     1801 gcggcggttc cagccgttac attccgcaga cggaaaaagg ttccaaacgc catgacaatg
     1861 aaaacgtcat ccaggaattc aaaaataaag gctacacctt cgttacgaac tcgaaggaaa
     1921 tgatggctgc cgacagtaat aaaccgctct tgggcctctt ccatccgagc acgatgaacg
     1981 tttatatcga tcgtgaaatg ctcaagaatc ctgaagtatt aggtaaatat acggatcagc
     2041 cgaacctcat caacatgacg aaaaaatcct tggatatcct cagcaagaat ccgaagggct
     2101 tcttcgctat gatcgaaggc gcatccatcg ataaacagct ccatgccatg gattggcagc
     2161 gtgctacgta cgatacgatc gaattcgata aggctatcga atatgctcag aaatggaata
     2221 aggaacacgg cgataatacc ttgatcatcg tcgttgctga ccatgctcac ggtgcttcca
     2281 ttaccggtac ctaccatgaa cacgacggca agaagggcat tgaagctgtc cgcacctatg
     2341 ccgattccat cttcccgacc ttcaaggatg ccgaccacga cggtttcccc gataatccgg
     2401 atcccgatgt aaccttggct gttcagtatg ctaaccatcc ggaatattat gaaaactacc
     2461 acttccagca gaaaccgacc tatccgacga tttcccagac tgttaccaaa caggaagctc
     2521 aggttgccgg cgataagacc gaataccatc aggtttcgac cgtcgttgct aaaggcaatc
     2581 cggctcgtca ccacgcaggc gatcccatgg aactcgttcc ggccaacatc cccgctacca
     2641 ttggtgatga aactcacgca gccgatgatg tcccgttgaa tgcagaaggc ccgggttccg
     2701 aatacttcaa aggtgtcatg gacaacacgg aagtattctt cggtatcatg cgtgcccttg
     2761 gcgtcgacgc tacgaaaaac gcaaaataaa aacggactac gatagcaagg cttatcttgc
     2821 tcggttacac cgccgtttca taaaaaatgc tgccgcaatt catattgcgg cagcattttt
     2881 ttgcttactg accgagaacg ctgtcgaggt aggcagcggt atccgataag aagctggccg
     2941 gataaccttc gatatcgccc ttatcgcagt gagctgcgaa ttccggatcg atgggcagtt
     3001 ttgccgtatg aggaatcgaa tatttctgcg ccgattcttc gacatggctg tgtccgaaga
     3061 tttcgtgttt ttcctggcag ctgggacatt tgaagtagct catgttttcg atcaggccca
     3121 agacaggaac gtgcatcatt tccgacatgt tcaaggcctt ttcaacgatc atggaaacga
     3181 gttcctgagg agacgtgatg actaagatgc cgtcgacggg caaggactgg aaaaccgtca
     3241 aggggacgtc gcccgttccc ggcggcatgt cgacgaacat gtagtcgatg tcgccccaga
     3301 tgacgtcggt ccagaactgt ttaacggcgc cggaaataac cggtccgcgc catacgaccg
     3361 gagccgcgac atcttccaac agcaggttca tggacatgac tttgatgccg gtcttggtcg
     3421 taacggggtt gatgccgtca tcgttgcccg tggcatgacc ggtcaagccg aagactttcg
     3481 ggatagacgg gccggtgata tcggcgtcga ggatagcagt tttaaagccg cggcgctgca
     3541 tttctacagc catcagggac gtaaccaagg atttacctac gccgcctttg ccgctgacga
     3601 cggcaatgac gtgcttgatg tgagaacctt cgtgaggcgc tgcctggagg ctctgaggct
     3661 ggttgcgctg tgcgcaggag ctgccgcagc tggaacagtc gtgtgtacaa ttttcactca
     3721 ttgggatcat tctcctttga acttttcttt ccggcacggc agcagtcgtg atggcacgag
     3781 ccggaacgtt cacaaacttg aatatggccg ccttcgatga cgaggtcttt gccatgaatg
     3841 agggcgtcgg ccagtttgtg gcgggctgaa ttataaatac cggtcaccgt cgtgcgagct
     3901 acattgatct gagcggcaca ctcttcctgc gtcagtccga cccagtcgat gaggcggatg
     3961 acttcatatt catcgattcc catggcaacc gtatcgtccg actgacgtcg gtcaaccggt
     4021 ccgaaggctg atgaaacagg cagtgaacaa acacgtcggc accgcggtgg tctcggcata
     4081 aaatccctcc tttacgctat ttattatagt tagatagctg acatatgtca aatttgataa
     4141 gcgttgccaa catattcttt attttccgga tgaccttgtc atcgtcgaag aaagtggtgc
     4201 agtttttctg aaagtagtag agaaaaccga attattctgt gataggaaac agcttttact
     4261 gtcttttaga tatagatgaa atgccgttta tcaataaaat ctatcaatgg gaggccgtta
     4321 aagttcccgg gtttttaaac ggattttata ataaatgaca gacgttaaaa gccaagattt
     4381 tctctagctg ttttactagg aaaggcttgg ctttttatga tatcattggt gattttttat
     4441 tttacttaca atgaactctc gcagtgctcc gacgtggcat atcattgaat tttcagcact
     4501 gataggatcg tgacgggcaa ttgcttctac tgtttttcgg tgatagatat acgtgttgct
     4561 gtcttttagg gaatcagaag tggcgacaat cgttttccta acagaggagg ttaaaatata
     4621 ggataaattg atgagaatac ggttaccgct ggcttcgcag atcatcgtat gcagcttgac
     4681 atcttctttt tcgtagtctg catttgactg tattaatttt tctac
//
LOCUS       DAWDXZ010000087         4694 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4761_length_4694_cov_8.762018, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000087 DAWDXZ010000000
VERSION     DAWDXZ010000087.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4761_length_4694_cov_8.762018"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2827..2836
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1099 a         1203 c         1252 g         1130 t
ORIGIN      
        1 gtactgaaaa tggcaaaatg agtatacccc aaggcgacag cgatgacgac gtaggcaacg
       61 gccgtaatcg agcatgaacc ggctgccaat ccgtcgagtc cgtccgtcag gttgacggca
      121 ttgctggttc cgacgatcat gacgaatacg aagagatagt agaacaagcc taagtcaacg
      181 gtcgtcgacg tgaaaggaat ccacaaggtc gtgggaaaat gaaggaaggc ttggaggatg
      241 tagcagaaaa cggcagccat gacgaactgg ccggccagtt tctgttttgc cgtaaggccc
      301 aaattgcggt gtttaacggc cttgatggag tcatccaaaa aaccgatgac gccgtgaccg
      361 agggtcagga ataaggccaa ggcctttctg atttccatcg gatcaaagag gaggcaagcc
      421 acggtcagcg ccgtcaggat gagaatgccc cccatggtcg gtgtaccggc tttatactta
      481 tggctttccg gcccttcttc tcgttcagac tggcgggcgt ggaactgttt cagttttttg
      541 atgacgaaag gtcccaggac aaggccgatg accaagctga cgatgaagct gaaaatcagc
      601 attttaaaca ttcacattac ttcctttcaa aatagggcag tattttttcc atagcaaagc
      661 tgcgcgagcc tttcaaaagg acggtgtcac cgtcccggag caaggccttc agctgacggg
      721 ccgccgcttc atgagaatcg gtcatataga cggctttcat gccggcatcc tgtgccgcac
      781 gggcgatatc ttcagccaga ttccccatgg tgatgagggc gtcgagcttc atatcggcaa
      841 ccaagcggcc gatggcccga tgctctcttt cggcccagtc gccgagttct cccatgccgc
      901 cgaggacggc gattttgcgg ccctttgtca ggagatccaa ggtacggata gcttcggcca
      961 tcgaagccgg attggcatta tacgtatcgt tgatgaaggt ataaggcccg aggtgcagga
     1021 gttcctggcg catggccatg cctcgatagg cagaaagtcc ggactgcagt tccgcgtcac
     1081 tgagaccggt caggtatcct gaggcaatgg ccgccaaggc attatagacg ttgtggcggc
     1141 caatgaccgg aacggaaaca tcaaaggtac ggtcgaagca gcggcaggta aagcgaaccc
     1201 ctttttcgtc acagacgaca ttctcggccc ggatctgagc cgaatcatcg atcccgtaga
     1261 ggacagaccg accttggcag agatccttca tcggtgcgac aaaaggatcg tcaccattta
     1321 agatggccgt tcctttggca tcgaggcctt ggaccaattc agacttggcc ttggcaatat
     1381 tttcctgaga acctaagagt tcgatatggc tcttgccgac gttggtgacg acgccgatag
     1441 tcggctcgcc gatatcgacc agttcggcaa tctgcccgag gccgcgcatg cccatttcga
     1501 cgacgcaggc ttcgtggtca gccgtcaact gtaacaaggt tttaggcagt ccgatttcat
     1561 tattataatt cttttccgtc tgcaatacct tatatttttc actcaagacg gcggcaatca
     1621 tattgcgcgt cgacgtcttc cctactgagc cggttacggc aatgacggga atggaaaaac
     1681 ggcggcggtg aaaacgggct aaatcctgat aagctttcag cgtatcagaa acgacgaaaa
     1741 tcgggaccgc agccccctta tattccggcc gcagttcaga gacgacggca ccggccgcgc
     1801 cagacgagag tgcctttaag acgaagtcat gaccgtcaaa agaagtcccc ttaaaggcga
     1861 cgaaaaggga gcccttttcc accgcccggg tatcggtgca aacgtcatga aaaagcacgc
     1921 tccctataga agagagcaga cgggctcccg tcgctttctg tacttcttca ctggtaaaat
     1981 cagccattat ttatctcctc gatgcaggtc gcgaacggca ttgcgggctt cttcgcggtc
     2041 gtcaaagtga atcgtaccgt catttaagat ctgatacgtt tcatgaccct taccggcaat
     2101 gacgatgatg tcattttctt gtgccagttc gacggctcga taaatggctt cacgacggtt
     2161 gacgatgact tcatgatgct gtcccgcctt aagcccttgt ttaacgcctt cttcgacttc
     2221 tgctacgatg gcatcggggt tttccgaacg cgggttatcc gaagtgacga tgggaatatc
     2281 ggcaagttct gcgccgatgc ggcccatgat ggggcgcttg gtccggtcgc ggtcaccacc
     2341 gcatccaaag acggcaatga cgtggttggg gttcatggcc cgtgccgttt ccaaggcctt
     2401 ttcaaggctg tccggcgtat gggaatagtc gacgacgata gcaaagggct ggccggcttc
     2461 gacgagttca aaacggccgg ctacggagtg gaaagtctga atggccttgt cgatagtggc
     2521 ttcgtcgatg ccttcggcat gggccgcacc gatagcagcc agtgtattgt aaacgttgaa
     2581 caggccggta atgttcatat tgagatggac attgacaaaa ggaccgacga cgtcgaagga
     2641 cgagcgtttc agttcgacat ttaaattcga acctttgagg tcggcatctt gttcaacgcc
     2701 ataggtcaaa acctggcagt ggcaagcatc gagaatctgt ttagaccagg gatcgtcgac
     2761 gtttacgata gccgttttat tgtctttgac atggtcggca gcggtcaggc tctggaagag
     2821 cttggcnnnn nnnnnnatac cggcgacgcg gtctaaggcc aaggcgtggc tggacacttc
     2881 catgacgaca tgggtcatgc ccttttcata catcagggcc aaaaagcgct ggagttcgac
     2941 gacgtccggc gtcgtgttgt gaatgggcag ttcttcatcg tcgataaggg cgtgaatcgt
     3001 gccgatgacg ccgacatgat ggccggcaga acgcagaata tgggcaatga tgtgggtcgt
     3061 cgtcgtcttg ccgttggtac cggtgacgcc gatcatgcgc atctttttgc cgggatagtc
     3121 ataaaagaaa gggaccatgt cttctaaggc atggtggata tcggggacct ggatctggac
     3181 cgttttcgac ggcaggtcga ggtgtttcgt cgtcaaaaca gcgacggccc ctttttcgac
     3241 agcctgcgct gcaaaagcag ccccatcgac atgagcgccg gaaatgcaga cgaataagta
     3301 gcccgattcg atgtcccggg agtccgaaga aataccggta actaccgtat cggcactgcc
     3361 gctgagatta taaattcctt taagttgttt gcaaagttca cctattgttt ttgtcattat
     3421 tatagaacct ccttgacggt tcatttcatt gtactgttct acattgtaca gctttatcgg
     3481 ataaataaca atgaggtcgt cgcacaaaag tgcgtcgacc tctcatttaa gcaattatcg
     3541 acggttcccc tttgcggcct gatcgctttg attcccatag agaatacgtg acggtgccac
     3601 cattcccagc tgtttcgtcg ccgtcgtata aattcgttcc ggcgtttcca atttggaaat
     3661 ttggacccgt aactgctcat tttcacgttg taactgctgt gcttgaagtt ttaattccat
     3721 gagttcgcca ctctgctgat acgtccaaga cgtaataaga ttagaagcga tggcaatcgc
     3781 tgcgatcatt ccaatgggaa ccagaacata tacggcaccc tgctggacag cctgtaccgc
     3841 agctgacctc cacaggggaa cgcttctttc ttcatgtaaa tttccgactt tccttgccag
     3901 catacgcaac acatccattt caataatcat tagcttggct ccttcattta cggataccga
     3961 cgcgaagttt tgcactgcgg gccctcggat tcaaatccag ttcctccggc gacggtaaaa
     4021 tgggttgtcg ctttactttt aggatggcct gatgatggca ggtgcaaacc ggcatatcgg
     4081 gcggacagat acaatcggtc gccaaaagac gcagcgtctg ttttgcaata cgatcctcca
     4141 gtgaatggaa ggtaatgaca cacaaacgtc ctccgcgctt taaacgctga gcggcatgtt
     4201 ccaaggaatc ctttaagatt cccaattcgt cattgacttc gattcggata gcctgaaacg
     4261 ttcgcttcgc cgggtgcggc ccgtccttac gagcgccggc cgggatggct tttttaataa
     4321 cgtgaaccag ttcttccgtc cgttctaagg ttttttcctg acgttctttc acgatgaatt
     4381 cggcaatgcg cttagcccac cgttcttccc cataatcttt gatgatacgg tacaagtcgg
     4441 cttcactgta accgtttacg acgtcgtaag cgctgcgccc ctggctgcgg tccatgcgca
     4501 tgtcgagggg cccgtcctgc atataagaaa atccccggtc cggtgtatcc agttgatagc
     4561 tcgatacgcc gagatcaaac atgatgccgt cgacggcatc gatgtgaagt tcgtcgagga
     4621 cttccgatat atacctgaaa ttgcgcggca cgagaatatg tcggcacggc gtatcagaga
     4681 ggcgttccgt tgcc
//
LOCUS       DAWDXZ010000088         4691 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4765_length_4691_cov_9.228214, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000088 DAWDXZ010000000
VERSION     DAWDXZ010000088.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4691)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4765_length_4691_cov_9.228214"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1178 a         1128 c         1245 g         1140 t
ORIGIN      
        1 atacggaacg agctgcctgc gtcctggcta ataaattgag ccgccgcggc gtccgtgata
       61 ttcgcatgta cgacgttgcc aaagtccatc cttcttttgt tacggctaag gcttgggaat
      121 acagccactt gatcctggct gcaccgacgt ataacttgaa cctcttcctg cccatggaaa
      181 acttcctcca cgacttgaag actctcatgt tccagaaacg caccatcgga atcgttgcct
      241 gccacagctg ggcatcggct gcgtataaga ctatggccga ttacgtagaa aacgaattta
      301 agtgctgcga actcctcgaa ccgacgatgg atctgaagtc gtctatgcgt gacgatcagg
      361 aatgcctgat ggatgaatta gccgataaaa tggcagaaag catcgaacag tatcccgatc
      421 cgaaaacctt gatctaagga ttaatcaatg agctgtccat catgaaaaag ggcttctgag
      481 tcgatttttg actcaagaaa ctgtctgttt catggtgggc agttttttct ttcactaatg
      541 cgaacatttt ttcgataaat ggcgtcattt gtcttttagg atgacccgtc gatgtggtat
      601 aatatataaa atacgatagg aggtggtacg atgatcattc gtgacgacag tgccaaaagc
      661 ggtgacaagt acgaacagga caaagcccgc agcaacggcg aagagctgca catcgacttg
      721 ggccgtgatt tgacggaaga cgaacagaag atatttgaaa aactgcgtga aggtttgaat
      781 ttataatgga caaggggact gcttgagaaa gcccatgctt tcgtcgggca gtctctttta
      841 aacccataga gctgcagctg cacgaggagg cgagagggtt gtggattggg aacgaatcga
      901 agaaaaatta catattttaa gcgatgctgc gaaatatgac gtgtcctgct catcgagcgg
      961 cagccacaga gcaaataagg ccggcggcat cggaaatgcg gcggcctgcg gcatttgtca
     1021 cacctggtcg tcagacggac ggtgtgtatc cctcttaaag atcctcatga ctaatcactg
     1081 catctacaac tgtgcctact gtgtcaatgc tgcgagccgc gacgcgaaaa gggctatttt
     1141 gacgccggaa gaaatcgccg atgtgaccat tgccttttac cggcgcaact atattgaagg
     1201 gctgttcctc agctccggtg tctatcgctc gccggattat acgacggagc tgttgattcg
     1261 gacggcgcgg atcttgcggg aagagaagaa atttaatggc tacatccata tgaagggaat
     1321 tcccggtacg agtgaggctt tgattcgaga actaggccgt tacgtcgatc gcttgagtgt
     1381 caatatcgaa ctgccgtcct cgtcgagctt aaaactcttg gcaccgcaga agaccaaaga
     1441 gaatatcctc gttcccatgg gtcatatccg tgaaggcatt gatcagtggc gtgatgaaaa
     1501 gaaacggtac aagaaggtgc cgtcctttgt gccggccggt cagacgacac agctcatcgt
     1561 cggggcgacg ccggacagcg accggacaat cctgcgcctc agccaggcct tgtatgacaa
     1621 ggtcgatatg aagcgcgtct attattcggc ctatgtgccg gccgtgacgg gttccaatct
     1681 gcctgctatt gcagagccgc cccttcttcg ggaacaccga ctgtatcagg ccgactggct
     1741 gatgcggttt taccagttta acgtcgaaga aattttgacc gatgaggttc caaacttcga
     1801 tttggatctc gatccgaaag cttgttgggc cctgcgccat ccggaagttt tcccccttga
     1861 agtcaaccgg gcttcgtacc atgatttgct tcgcgtgccc ggcatcggcg tcgtgtcagc
     1921 ccgccgtatc tgcgctgccc gccgtcaggc cttcctgtct tatgacagtc tgaagcgctt
     1981 aggcgtcgtg ttaaaaaggg cccgctattt tattacctgc aaggggaaat actacggtct
     2041 ttcgacggat ccggcctatg tccgaaggat gctcttatcg ggacagaagc agccgtctgc
     2101 ttacgagccc atgagtttat tttagaggca atgctatgat atatacttac gatggaagtt
     2161 ttttcgggta cctgtcggcc gtctttgacg gctggcatga cggcgttcat caaatcgagg
     2221 atatctgttt ggaagccgag gaggcctcac ttttttccga gtcacgcttt gttacgactg
     2281 acgacggcaa ggcacggcgc atcctggacg gccttttgcg gcaatgcggc cccaaggcct
     2341 gtcattattt ctactatgcc tttttgtcgg aaccgaaaaa aggcgggatg atcttactgg
     2401 gctatttgcg gaaggctttt tattataaag ggacatttct caatcaccgc agcgaggccg
     2461 atatttggac tgtctgcgag tgggcacgga agacgggtaa cgagcgccat aaactcttgg
     2521 gactggcccg ctttcaggaa ttgtccgacg gcatgcttta ctgccgactg aagccgacgt
     2581 gctgcgtcat tccggtcatg gcaccgcact ttatccgccg ccttccgggc gaacgctggg
     2641 ttctccacga tacgggccgg gggtttggtg tctattacga ccgggaaaag cccgtcatgg
     2701 tgacgattcc tgacgtacag gcggaaccgg aattgtcaga tgatgagctg gccattgaac
     2761 gggcttggca gaattattac cgcacgattg ccattccttc gcggaaaaat gaggccctgc
     2821 gccgcaattt catgcctaaa aaatattggc cgaccctcat tgaaatgaaa tgaaaaaaag
     2881 tgtttctttt tcccggcact tgatatataa tagtaacatt ggattggtga taggaaaggt
     2941 ggaatacatt gaaacctgtc ttagtaagtg tacagagtat ccaacgcgac ccggaagggc
     3001 aagagctgaa aatagagctc gtttctgaag gtaagtatta tatcaaagac ggtactcagt
     3061 atatcgtcta cgaagaaagc gaactgacgg aaatgaaagg cgttacgacg gtcatcaagg
     3121 tcttacccgg agggtccgtc ttattgttgc gtctggggaa acttcgccag cggcaggaat
     3181 ttcataaggg aaaggtaacc tactcccagt atgaaacacc ggttggcacg tttgacgtga
     3241 agatgaaaac ctatgaatgc cgtaccgatc tccaggatgg tatcggcacg atctatctgg
     3301 gctatgacgt ccatattgaa gatatcagtt ccatgtatac tcaattaacc ataactgtgc
     3361 aggaggataa agcgtaatgg aaatgaagga tttattgaag cagggaatta cggaagcctt
     3421 gaataaggcc atggataaag ggacgcttcc ggccggtgat tatccgagtg ttgccttgga
     3481 agtgccgccg cagaaagagt tcggcgactt tgccagcaat atcgccatgc agtcggcccg
     3541 cgttgcccat aaagctccga aactcattgc ccaggccatt gtcgatgaaa tggattatcc
     3601 ctggctggaa cgagctgaaa tcgccggagc cggcttcata aatttctttt taaagaacga
     3661 cgtcatttac gatacgctga aacaaatcct ggccgccggc gatgcttacg gccatgctcc
     3721 tttgcgtgag gacgattcgg tccaagtcga atatgtcagt gccaatccga cgggaccgct
     3781 ccacgtcggc catggccgcg gtgccgccta tggcagtacc ttggtcaatc tcctgcgggc
     3841 agccggttat aacgtccagt ctgaatacta tatcaatgat gccggaaacc agatgaataa
     3901 tttggcggcg tcggtcaatg ctcggtacct ggaactcttg ggccggccgg ctgaaattcc
     3961 ggaaaatggt tatcacggcc acgacatcat tgaaacggct caggccatta ttgatcagga
     4021 cggcgataaa tacctcgaca tgccggaaga agaacggctt gacttgttta aagaccgggc
     4081 ctatgtcgaa aaactgaagg cccttaagcg ggatttggaa cacttcaacg tttcttatga
     4141 caattggttc agcgaacgga cgctccatcc ggacgccatc aaggcggcct gcgatgttct
     4201 gcaggggcgg gggaaaatct atgaaaaaga cggtgccttg tggctgaagt cgacggatta
     4261 cggcgatgat aaggaccgcg tcgtcatccg tgataacggt gttccgacct atttggctgc
     4321 cgatattgcc tatcataaga ataagtacga tcggggtttt aaaaccatga tcaatatttg
     4381 gggtgccgac catcacggtt acgtcgcccg cgtcaaggct gccatggcag cccttggtta
     4441 tgatacagcc caattggaag tccttttact gcagatggta agccttttcc gagacggaca
     4501 gcaagttaag atgagcaagc gtaccggtca ggccattacc ttaaatgaac tcatcgaaga
     4561 agtcggcacc gatgcagccc gctacttctt catcatgcgg tctctcgata cgcagctcga
     4621 tttcgatttg gatttggcta aatcacacag caatgaaaat cccgtttact atatccagta
     4681 tgctcacgct c
//
LOCUS       DAWDXZ010000089         4686 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4773_length_4686_cov_7.825092, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000089 DAWDXZ010000000
VERSION     DAWDXZ010000089.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4773_length_4686_cov_7.825092"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    498..507
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3011..3020
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1069 a         1143 c         1194 g         1260 t
ORIGIN      
        1 ttttttcacg atatgtgcct gtgctaagtg acggttgcag atacccatac ctttcagtcc
       61 gagctgaaag gtattttttt atgtcctttt gccccgaaac taagacagga ggtatagatt
      121 gattgaaaaa gtgttgtgat tttctggtca gccatatggc cgtcttcgtc gtcctcatcg
      181 gcgttgtcgg agcgctgtgg ccgtggacgc tgaagtgggc tgctccttat gtcggctggc
      241 tcttgggcat cgtcatgttc ggcatgggca tgaccttgac cgtcgcggac tttaagactg
      301 tttttaagcg gccttgggaa gtccttatcg gcatctttgc gcagttcctg atcatgcctt
      361 tcgtggcttg gggcctggtc cgtatctttg ccctgccgcc ggaactggcc attggcgtca
      421 tcttggtagg tacctgcccg ggtgggacgg catcgaatgt catttcctac ctagctaagg
      481 gcgacgtagc cctgtcgnnn nnnnnnntgt tggctcccat cgttacgccg gctttgactt
      541 ggcttttggc gggcgcatgg atccatgttt ctttcttgtc gatgatgctt tccattgtcc
      601 agatggtcct ggttccggtg ctgctcggct taggggttaa ccatttcttt gcccgcggcg
      661 tcgaaaaggt actgccggcc atgcccgtcg tctccgtggt caccatcgtc attctcgtag
      721 gctgcgtcgt atccctcagt gcatctcgcc ttttcgatgt cggcctctta atggcggtca
      781 tcgtcgtcct gcacaacgtc ttcggactga ccctcggcta cctcatggcc aaactgttcc
      841 gcctcgattc gaaaaaatcc cggaccgtcg ctatcgaagt gggcatgcaa aattccggaa
      901 tggcagcgtc cttggcggtc ttgtacttca atccggcagc ggctattccc ggtgctattt
      961 tcagtgtctg gcacaatatt tccggatccc tcattgccaa ctacttctct cgccgcgatg
     1021 aaaagggcga tggcgtcggt gttaaagaat cgacagccca tgcggcataa tctattttta
     1081 taaaggagtt tatgatcatg aaaattttcc gtactgtcaa agaaatgcag gcattttccc
     1141 gtgaacaaaa gaaggccggc aaggtcatcg gcctggtccc gactatgggt gccctccatg
     1201 aagggcattt gacgctgatg cggacggcaa aagccgcctg tgacgtcgtc gtcgcttcgg
     1261 tcttcgtcaa tccgacccag ttcggcccca acgaagacta tgacgcctat ccgcgccgct
     1321 ttgaagaaga ctgccggaaa ctggaaagtg tcggcgtcga tgccgtcttc catccggaac
     1381 cgtcggaaat gtaccccaag ggttactgca cgtacgtcaa tgtcgacggc gatattaccc
     1441 ataagctctg cggtgcccag cggcccatcc atttccgcgg tgtggcaacg gtcgtcacca
     1501 agctgatcaa tattaccggc gccgatgaag ctttctttgg ccaaaaagat gcccaacagg
     1561 tcgtcgtcgt ccgccgtttc gtcagtgacc tcaatctgcc cgtccatatc aacatggtcc
     1621 ccatcgtccg tgaagaaagc ggtctggctc ggagttcgcg caatgcttat atgtccgaca
     1681 ctgaaaaaga agcagccctg gtcctctcgc gcagcctgag aaaagccaag acggcttatg
     1741 acggcggcga aaaagatgta gaaaagctga aagccattgt tacgtctgaa attgaagccg
     1801 aaccgatggc ttccattgat tatgtcgatc tcttttcctt ccctgacttg gaaccgatcc
     1861 aaacggtttc cgacgaaagc cttttggcca ttgccgtccg catcggtaag acccgcctca
     1921 tcgacaatat catcttaaaa tagtaaatct ttatattttc ttgacatttc ctccatatct
     1981 ttttcgcacc attttggtat actctaacca tagaaatgat gatgattttc tttaggaggt
     2041 tatatctatg aaaaagaagg tactgcttat cgtagctgct ctttccgtta tggcaggatt
     2101 ctctgctttt gcagccaatt catattcaga ccagaatgat tcttcttatt acaacaatgg
     2161 ttattactgt cacggcaatg gctactacag tgacaatggc gatggctacg acgatggcta
     2221 ttgccatggt ccgtacatgg gccaccatgg ctatcagaga taatcgtttg ttttaaaagg
     2281 gggaagttaa tatggtaatg actcattaca tggagctctt gtcgcttcat tcaccgtggt
     2341 ttttgattct cttcatgctg gtgccgatgg tattggcaga aacgattttg gcatcgggtg
     2401 ccttttcctt gctgtataag gatcatcgca gcgaaaaatg ggacagcctg agccacatct
     2461 gcggtattat tctcggggta ttcttcatca ttgcgacggt atatatcgtc acgtcttaca
     2521 ttccgaccat tcagtggcgc ggtcctatcg acatggtttc gatttgggct tacgtcctcg
     2581 gcgtcattcc tgccgttttg attctcttgc aggaattggg gattatcttc aagaaccagg
     2641 attctacgtc taagatcaag aatcatatcg tgctcatgat tctcttcgtt ctgttcaccc
     2701 acttggcgat ggtctttggg atggcagatc cgaaactggc cggctacgtt ccgacgcagc
     2761 aggaaatgca gatgcatgac aacatgcaga tggacatgaa tatgcctatg gatcacagcc
     2821 agatgaacca tgaccagatg aatcacgacc agatgagcaa tggtcagatg aaccacgatc
     2881 agatgggtca tgaccagatg agtcacgatc agatggattc gaataaataa gatttcgtgt
     2941 attttcaatc ataagaatgg actgcttctt cggaggcagt ctttttttga tggggtcgct
     3001 cctatagagg nnnnnnnnnn cggaaccaaa agccgcagat catgctgctc gatttgaaga
     3061 tgcccggcat ggacgggttt gaagtctgcc gtaggacgcg gcagttcagt gatataccga
     3121 ttatttttct gtcggcaaaa gatgatgaaa cggatcgttt ggtagggctt aatatcggcg
     3181 gtgatgacta tgtgaccaag ccttttcacg ttaaggaaat cattgcccgg gtctacagcc
     3241 tattgcgccg cactcggggc gaggtcctgc agcagccgac gtcgtacatg attcgcgatt
     3301 tggtcatcga caaggagaag ttcaccgttc ttcgaaacgg ccagcctatc gtgctgacgc
     3361 cgacggaatt taacctcttg gaagtcttgt cggcttcacc ggaccgtgta ttcagccgca
     3421 tgcagctcat ggataagata cagggaggat acagctttga gggctatgag cggactatcg
     3481 atacgcacat ccgcaatctg cgcaaaaagc tggagcctaa tccggccgaa cctacgtata
     3541 tcttgaccgt gtacggtatc ggctataaat ttgtaggagg cagccatgaa gaggtctgac
     3601 agcatcgtga tccgcctgac ggctttggta tttattctgc tgcttttgac gattgctatc
     3661 ttgctcattt tagtcaataa tcagatgaat aaccattttt cccaatacct gaccaatatg
     3721 tctcatatga tgggcaatgc ccagaacggt atgggaatgg gcatgagcgg tatgcacgga
     3781 tcgatgcacg ggccggcaga gagtacttat atttctgctg tccaccagtc cttgctgtgg
     3841 gtcggtatcg gcatgatcat cgtcagtgtt attgtcagct attttgtcgt tcgggaaatc
     3901 atgcgcccct tatcgaccct gaccggtgcc gtacaaaaaa tcaggaccgg aagttacggt
     3961 caaacggtat ccgtggaacg ccacgatgag gtcggtgtcc tgacggagat gtcggaagaa
     4021 ctggcgcgaa atgacaagat gaggcggcag ctatttgcta atattgccca tgagcttcgg
     4081 acccctctgg ccattctcca ggggaattta gaaggcatga tcgatgatat tattcccacg
     4141 gataaaaaaa tactcttgtc tatggccgat gagacggtgc gcatgggtcg ccttattcag
     4201 gatcttcggg acctgtcctt ggcagaaatc gatgaattga ccctccataa ggagccggcc
     4261 gatatcaata cgatgttaga acgggcggtc agtatgcttc agcctttatg tgaggaaaaa
     4321 tcgctgacag ttcgacaaaa cctaagtcgt gatctgccgt ctcttgtcat cgacgtcgat
     4381 cgcatcaatc aggtcattta taatttactg aacaatgcca tccgctatat cgacaagggc
     4441 tgtacgatta ccgtttcaac catgaaggtt gccgtagagg ggaaacctta tgtccaggta
     4501 caggttgccg atacgggaaa ggggattgct ccggaagatt tgaatcatat ttttcaatac
     4561 ttctatcgca gtgagcagtc acggaatcga aaaagtggcg gaagcggcat cggcctcgct
     4621 ttggcacagc agtttatccg cagccacggc ggcaacatct gggctgacag tacggtcggc
     4681 aaggga
//
LOCUS       DAWDXZ010000090         4660 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4791_length_4660_cov_6.662975, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000090 DAWDXZ010000000
VERSION     DAWDXZ010000090.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4791_length_4660_cov_6.662975"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4068..4199
                     /estimated_length=132
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1047 a         1265 c         1133 g         1083 t
ORIGIN      
        1 ttacggcatc aagcgcggcc tttactcgaa cgaagccggt atcggttcgg ctccgaatgc
       61 ggcagctgca gctgacgtca gccatccggt caaacagggg ttggttcaga tgttatcggt
      121 cttcatcgat accctcttag tttgtagtgc gacggctttc atggggcttt tctccggcgt
      181 tcccatcgtc aaagaagcag ccggtgccgc ttatgtacag agtgccgctt cggccgtctt
      241 cggcggattc ggaccgctcc tcattacggt ttgcatgctt ctctttggtt tctcgacgct
      301 tatcggcaac ttatactacg tcgataactg tatccgcttc atccataaag gggctccgtc
      361 tcagagcttc gtctatagct tccgcatcgt ctgcatcgcc atcgtcttca tcggtgccgg
      421 catgtcaatg gccgccgtct gggctattgc cgacatcctg atggccatca tgtgcttcat
      481 caatatcccg gcctgcttta tcctcagcaa agtcgccgtc aatgccatga aggattatca
      541 gcagcagcgt gaagcagggc aaattccggt ctttaaagcc gctgctatcg gtatcgacga
      601 aagcacggta cagtattgga aataaatcct cttgacaaat agggggagag accttacaat
      661 acgcttatat acaatgataa ttgtattaaa taattaagcg taaaagctat gaacaagacg
      721 gccgatatgg cgcagccttt ttagcgaacc ggggatggtg aaagcccggc agaggctgta
      781 tattggcgga tcacttggga gctgcagctg tgaaccagct gccgggtcgc atcgttatat
      841 tgcgtcaaag tggctgcgtg agcagtcaat agggtggtac cacggtgatt acgtcttcgt
      901 ccctttttgg gacggagacg ttattttttt tcacacagat aagaggagga agcatcatgg
      961 attacggtaa gactttacat ctgccccaga cggaattccc catgcgcggc aatttgccga
     1021 aacgggaacc ggactttttg aaattttggc aggatcatga catttataaa aaacgcttgc
     1081 agaaacggga cggagcgcct aagttcattc tgcacgacgg ccctccctat gccaatggca
     1141 agattcatat cggccatgct ttaaacaaag tattaaaaga cattatcctt aaatataaga
     1201 cccagcaggg ctgctatacc aagtatgttc ccggctggga tacccacggc cttccgattg
     1261 aacacgccgt catcaaggat acaggcctca atcgccacga aatgacgccc cttgacttgc
     1321 gtgctaaatg ccgcgattac gccttagccc gcgtcgaaga acagaagaaa gacttcatcc
     1381 gcctgggcgt tctcggcaat tgggaagatc cctacctgac ccttcgcaaa cacgtcgaag
     1441 tcgctcagat cggcgtcttt ggcgaaatgg ctaaaaaagg ctatatctac aaaggtctga
     1501 agaccgttta ctggtgcccg cactgcgaaa cggctctggc tgaagctgaa atcgaataca
     1561 acgacgataa atcgttctcc atctacgtca agttcaaatc cgtcgacgtc agcggccata
     1621 tgccgaaaga tgccgatccg gaaaaggtat ttgccctcat ttggacgacg acgccttgga
     1681 ccattccctg caacctggca atcagtgcca acgaaaacct ggaatacgtc tgggtccgcg
     1741 tcggcgatga atatctcctg atggctaaag aacgagttgc cgttaccatg gaggccggta
     1801 aggttacgga ctatgaagtt ctgcccgacg tcatgacggg caaacagctc gaaggcatgg
     1861 tcttcatgca tcccttctac gaccgcaagg tcccggtcat cctcggcgat cacgttacgc
     1921 tcgaagccgg cacgggcctc gtccacacgg ctcctgacca cggccaggat gactttgatg
     1981 tctgcatgaa gtatgaagcc tgggggctca aacctttggg cactgtcgac ggttccggcc
     2041 gctataccga taaggttccc ggctttgaag gccagttcgt cttcgatgcc aacgtaccgg
     2101 tcatcaagaa attggctgaa cagggcaccc tctttgcaaa aagcaccttc cgccatcagt
     2161 atccgcactg ctggcgctgc aagaacccga tcatctatcg ggcaacggaa cagtggtttg
     2221 cttccgtcga cggcttccgt caaaaggccc tcgatgccat cgatacggtc aaatggatcc
     2281 cgtcttgggg ccatgaccgc atctacaata tgatccacga ccgcggcgac tggtgcatct
     2341 cccgtcagcg tgtctggggc gtccccattc cgatcttcta ctgcgaagac tgcggggaac
     2401 acatcatcaa cgatgaaacg atttctcacc tccaggatat gtttgctaaa gacggttccg
     2461 acgcctggtg gatgtatgac gtcaaggaat tgatgcctga aggctataaa tgcccccact
     2521 gcggcggcac ccatttccgc aaggaaagcg acatcatgga cgtttggttc gacagcggca
     2581 gcacccatca gggcgtcctt aagcacgatc cggacctcga ttatccctgc gaaatgtacc
     2641 ttgaaggctc ggaccagcat cgcggctggt tcaacagctc gctcctcacg tccgttgccg
     2701 tcaacggcta tgctccgtat aaatcggttc tgacccacgg ctttaccgtc gacggcgaag
     2761 gccgcaagat gagtaaatcc gtcggcaaca ccgtagcacc gcaggaagtc atcgaaaaat
     2821 acggtgctga tgtcctccgc ctgtgggtat cgtcggctga ctaccagggc gatatccgct
     2881 tgtcgccgaa gatcctgaag cagctctccg acgtctaccg caagatccgc aacaccttcc
     2941 gctatctctt gggcaacttg gccgacttcg acccgtcgaa agatgccgtc gcctaccagg
     3001 atatgacgga actcgataaa tgggctctgc tgcgcatgga acaggtcttc gaagaagtta
     3061 ccgacgccta tgaaaactat cagttccacg tcatgtacca tgccatccat aatttctgca
     3121 ccgtcgattt gagtgccatg tacctcgacg tcatcaaaga ccgcctgtac acggaaaaga
     3181 cggatgctcc cgtccgccgc agcgcccaga cggcgatgta catcatcttg gatacgctgg
     3241 tcaagatcgt cgctccggtc ctgtccttca cggcggaaga agtatggcag aacatgcctt
     3301 ctgtcgacgg caaagaagaa agtgtcctcc ttacggattg gccggaagtt cataaagaat
     3361 atctgaatgc cgacctcgat gctcgttggg accacttcct gtcctatcgc cgcgacctga
     3421 cccgtatcct cgaaggagct cgtcaggaac acaagatcgg tcacgccctc gatgctgccg
     3481 ttaccatcta tgccgatggg gaagactacg atttcctcag ctcttggcag gatcagctgg
     3541 ctgccttgct catcgtcagc gaagcgaaac tcgtcaaagg cgaagctccg gccgatgccg
     3601 cagccggtga agaccacagc gacatgaagg tcgttgtcgt accgtcgagt gccgaaaaat
     3661 gcgaacgctg ctggatgcac accgacaccg tcggttccga tgccgctcat ccgacgctct
     3721 gcgctcgctg cgcccacgta ttaagcgaat aaattttcaa ccttgaaatt tccttatccg
     3781 taaggaagct catggttgtc ccgaaatgga tagtttatgc ctgtcaatga cagcataggc
     3841 tatccgtttt ttttgttgaa attttatgaa ttttatttta taataaatga agtgttctgt
     3901 tataatgtac atttgtattt ttggaacgat atgttctatg ggaggttttt ggtatgcata
     3961 gtttatggag tgccctaaat cgcatcagct tgatcaagca gatcatcgtc ggcctgatca
     4021 tcggcattgc ggtagccatg agttttcccg cagctgctca tgagttgnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     4201 gacgctcctt aagaatatcg ttgataatcc cgtccacgct ttgatgaccg gcaactacat
     4261 cggtatcctc ggctgggctg tcctcttcgg catcgccacc cgccgcgttt ccgaatcgac
     4321 gaaagccgtc ctcgacgact gtgctaatgc ggtcacgacc tgtgtccagt gggtcatccg
     4381 cctggctccc ctcggcatca tgggcctcgt cgccgattcc atttcttcca acggcattga
     4441 agccatcatc ggctacgctc agctcctggc cctcctggtc gggacgatgc tcatctttgc
     4501 cttcgtcgtc aatccgatca tcgtctatgt caagcttcgc agaaatccct atccgctcgt
     4561 ttggaagtgt atctcgacca gcggcgtcac agccttcttt acacgcagtt cggcggctaa
     4621 catcccggtc aacatggcct tggccaaaga actcggcatc
//
LOCUS       DAWDXZ010000091         4658 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4793_length_4658_cov_9.896806, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000091 DAWDXZ010000000
VERSION     DAWDXZ010000091.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4793_length_4658_cov_9.896806"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1141 a         1350 c         1167 g         1000 t
ORIGIN      
        1 gggcaacaga ctttcgtatc aactacttgg cccgtatcac cctcggtatc caagaagaaa
       61 tcatgaaaca aaataaaaca attcatttaa ccgttgtccc atataaagtt gaccagctcc
      121 aaaaacaact cgaagccatg cagaatgaat ttttcaacgg catcatcatc gctaacatgt
      181 ccaacaagga catggattat ctcaattcca tccatattcc ctgcccggtg gtcctgttca
      241 acagggaatc ggataaatac agcagcgtca ccatggacaa ctatcagatc ggctgcaacg
      301 tcgcccgtca tttcctcgac aagggcattt ttgacgccgg cgtcttaacc cacgacacaa
      361 ccttccccat catgcgtacc tggatgcagg gcttcctcga tacctttgcc caaaacggcc
      421 atccggttcc ggaagaaaac atcgtccttt gtgacacggc ttccgccagt gctgtcgccg
      481 ctacgaaaaa actctatgaa gaaaaacgga tgccccgggc gatgttctgc gaatcggacg
      541 tcctggccca cggcatgctc tatgcctgcc atgccttagg catcgcgatt ccggaggaag
      601 tagaagtctt caccatcggt atcaacatgg ctcagctcaa tgactatgcc atcccatcct
      661 tatctcgtgt cgatataccc atgggcgaaa tcggtcagca ctgtctgcat ctcatggtcg
      721 acctcatcga aaagaaaaaa gaaaatccga cggttcaatt cgtcgaaggc cgggaaatta
      781 tcggacaatc ttcaccggca gaataagtaa taattcaatt tctttccctc ccccttattt
      841 agcaaaagga acgaccccaa aaaaagattt ttacggggtc gttccttttc tatatccatt
      901 tatgcagcca tcaagactgt aaaaccgatt tcatcgccct tacgatgtcc gaaaattcct
      961 catcgccaag ggtccgtacg gaaaggataa gggcatcgtg acggatgcgg gccaaaacag
     1021 ggatatctcc ttttcgaagg ctttcttcta aggccgttac cgtcccgctt gtcggccgga
     1081 cagcaacgcc ccagccggaa aggacgacac cggggaaggc accgccgccg acggcatcgt
     1141 cgacttcaac gagcgatatt tcaaggggaa gaccggcctc cgataaagct gctgccagca
     1201 acttcccgcg tttatggcaa aggtcagaag gctgcaccag ggccgccaat gtcggaatct
     1261 tttgaaccgc ctctccgtcc cgatagatgc gaagcgtccc ttcgagaccg gctaaggtca
     1321 tcttatcgat gcgaagggcc cgaagcagcg ggtggcgtct catccgttca atataggaag
     1381 ccttgccgac gatgatgccg gcctggggcc cgcctaacag tttatcaccg ctaaaggtaa
     1441 cgacgtcgca gccggcttca atgacttctc ggaccgtcgg ttcatgaggg agtcccaatg
     1501 actccaagtc gataaagagg ccgctgccaa ggtcattgat gcagggaaga ttttgggcgt
     1561 gagccagtct gactaaatca gctgccgtta cgctttccgt aaagccgatg atgcggtaat
     1621 tgctggtatg gactttcatc aaagcgcccg tgtcttcacg aatagcccgt tcataatcag
     1681 ccaaatgggt cttattggtc gtcccgactt cacgaatcgt gcagccgctc tgttcaatga
     1741 catcgggaat gcggaaggag ccgccgattt caaccaattc acctcgggaa acgacgactt
     1801 ctttgccggg cataaggctg gccagtacca acaggaccgc ggcagcgtta ttgttgacga
     1861 ccagggcatc ttcggccccg gtaagctgac agaggagttc tcgtacatga tcatcacgaa
     1921 ggccccgttt tcccaatgct aaatcatact ctaaattagt gtagtgcccg acgagttccg
     1981 caatctgtcc ggcaatgtcc ggactgagaa cggcccggcc taaattggta tgaaggacgg
     2041 tcccggtccc attgatgact cgccgcaaat gccccttatc cgcgccctgt ataaaggccg
     2101 ccacggcttg ttcaaaaagc tccatagaaa aaggttcatt ccagagtcct tcgagaatgg
     2161 ctctacgcct atcgtcgaga acggcgcgaa tggcatcgcg aacaatatga cgcggttttt
     2221 ctaactgctg aaaagccgga cgctgcaaga gcgtatcgac atcaggcagc tggcgaagca
     2281 gctgttttat agcttcattc ataaacgtca tctcctttag ctgtatcatt agcggccctt
     2341 gacccgaaag cccggccatc accggccttc accgtaagac ccaaaccatc aaaataagtt
     2401 aaaatcaaga gggcctgacg gcggttaacc tgcaataggt ctcgcaacgt ccccaccttt
     2461 aaaacttcat gctccttgaa atactgagta acgacctcgg cggctcgtcg aaggcaggcc
     2521 ttcgataaga caaactccgg cgaaaggaag gtgaggctct taccctgtaa ggccttgagg
     2581 atttcccgta cgtccggccc caaagaaagg aggtcggcaa tcgcaacggg gcggtacccg
     2641 cttcgagcca atcgcctttc aatctccgac tggcattggc gctgacgggg tgaaaacgcg
     2701 aggcgaaaat ccttagccgc aacgaaattt cgcgacagtc ggcagatttt tcgcgccaag
     2761 agcaactgca gcagtgcccg accttctttt tcaccaaggc ctaaccgctg ccgccattca
     2821 ggttggggca tgccaaagcg caggggccac cgtttatggt actcatggag tacagaaagg
     2881 ctccgagcct gccatgactt gaaggcttcg ccatgaatcc agcccaggcg aaatggcttc
     2941 acccggcctt gatgttttaa atgttcgaga gccctttcaa cctccccaga aggcaggtcg
     3001 gttccggcag aaatagcctc aaccgtcgaa aacggagact gacagcactg taaaaactga
     3061 gcaatcactt cctctttcag attcgcatcg cgagctcgta aggccgtcat cagattctct
     3121 ttaaagcgac ggtgtttttc cggacgggca tcgaggattt ttcctccgcc tacggtatgc
     3181 atgggcgaaa acgaacgaag gataaaagga tccccgcttt tgacatagat ttcatctcgc
     3241 tccaaccgca gctgcaagaa accttccgta ccgggttcga tggcttcgac gcctaagggg
     3301 acgacacggg ccatgacttc ccgcgtcccg actaagaggc gcacacgctg ccagagtacc
     3361 agtggcggcg cctccctaag gcaggtcacc ttggcatcga tcatgcgcgt cgccgtcacg
     3421 tccggtaccg cacagaggac cgagccgcga ggaacgtcgc cgcaggaaat tccggccaag
     3481 ttaagtgccg tccgctgtga cggttccgcg cggaggacgt cttcctcata cacctgaatg
     3541 ttgcgaacgc gcagctgccg ccctccgggg taaagccata ggtactgctc cttttccacg
     3601 accccgtcca ggagggtacc cgtaacgata gttccgactc cattaagcga aaaagaacgg
     3661 tccacatgga gacggccatg accggaagcc tcttggggca gaatcgtttc ggccatccgg
     3721 gaaatggcat cgacgagttc acgcattccg cggccgctca aagcatcggt gacgacaagc
     3781 ggggaccctt ccgcagccgt accgatgaaa gcttggtcca tgcgggttaa ggactcctca
     3841 agggcttttg gggcaacggt atcggccttg gttacgacga cgataaattt acgaacgcca
     3901 aaaagggtca agatgccgag atgttcactc gtctgcggca tgaccccttc cttggcatcg
     3961 acgacgagca ggaccatgtt gaggcccggc aggccggcga ccatattctt tataaatttt
     4021 tcatgaccgg gtacatcgac gataccgcac cgcgtcccat cgggcagatc cagccaggca
     4081 aagcctaaat cgatagtcag gccccgtcgt ttctcttcag ccgtccggtc ggtctcgatg
     4141 cccgtcaaag cccgcacaag cgtcgtcttg ccgtgatcga catgaccggc tgtgccgata
     4201 acgatatgtc tggtcatcct gtcaaccttc ttttatctta gtatatgaaa cggaaaggcc
     4261 ttgacggcag gcatgatata aagcggccca ttccaccgcc tccttgtcga ataaggcagt
     4321 gagggccgct tcatcgttca actcgtattt caaagaaaaa ccacaggacg catcgatttt
     4381 tcccggcgta ggaatgatgc gcaccgaaag tcctgctttc ttcgcaaggc gttcaccttc
     4441 aatagcctct cgcgtcgacg gcactaagac gataccgtat tcctgaaccg ccattaccct
     4501 tcctgagcca aggcctgctc aacggcgcgt gccagctggt cggcacaaga cgtgccccga
     4561 tcctcgcagg gatttccttt taggatagcg acggcccgct tggcatcggc gtcttcaagg
     4621 agcttgccga gagccaaggt gttgccggga caaccgcc
//
LOCUS       DAWDXZ010000092         4611 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4840_length_4611_cov_9.260975, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000092 DAWDXZ010000000
VERSION     DAWDXZ010000092.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4840_length_4611_cov_9.260975"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2911..2920
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          846 a         1172 c         1273 g         1310 t
ORIGIN      
        1 tagttacctc tctatagttc aaaataaatg aagtataaaa ttaaaaacat cgattttatt
       61 taagcgtttg ttgatgctat actaacagag aaaaatcaaa ctgtcaatga gggagtctta
      121 tgattatgtt aaaaacaaca ccgaccttac agaaacccgg ctcactgctt cagagcgtcg
      181 tcctggtcat cgccgcctgc atcgtccacg gcgtgatgca aggcgtccac gacaattacg
      241 gcatcatgat gaccggactc attccctata cgggcatcga ctacgtgacc atcagctttg
      301 ctatcggcat aggcgccctg gtctatggac tggcccagcc ctttctcggc atcctggcct
      361 tgagacgatc tccggtcttc gtcattctgg tagggattct ttttaccgtc gtcggcttgg
      421 cggcgacgcc cttatgtacg agcccttgga cgctggtctt attttttggc ctcatcctgc
      481 cctttggcac gacgggcctt tgctttggca tcatcatggg ggccattacg ccgatgttgg
      541 gagagaaacg ggcggctgtc gtctcaggac tcgttcaggc cagtgccggc attggcgacg
      601 ccttcatgtc tccggctttg aatctggcca tttcaacctt tgatatcgcc tttgccatga
      661 gggctttgac cgttcccttt ttactgatga ttcccattgc cgtctggatg cactctgtcg
      721 gtaaaaagga aactcaacga gaagaggccg ttcacgaagg gaaggggact tcgaaagtgt
      781 tcctcattcc cctgctgaaa tttgccctaa aagaccggga ctatcgctta atcgtcatag
      841 gctttgcgac ctgcggcttt aacatgtcca tcattgaaag ccatctattt tcacagtatc
      901 tgtcctatgg catcgacggc agcacggcgt cgctgacact gaccgtctac ggtatcgcca
      961 ccatgatcgg tgccgccggg acgggctttc tcggagcggc tttcaagatg aagaacgttc
     1021 tggccggagt ctatgccgtg cgggtcggga tttccctggc ctttctgctt cttcccaaat
     1081 cggtggcctt tgcctttgcc gccacggcct tactggggct ctcaggagat gcgacggtgc
     1141 cgccgacgtc gggcatcatc agtaaaaagt tcggctcgaa aaacatggct gtcttgtatg
     1201 gatttacctt gatcgggcac cagataggcg cctttttcag cgcctatctc ggcggccttt
     1261 tcgtagatat cggctggggc tactcgcccc tttggctggt caacttctgc ttggccgcta
     1321 tggcggcaac ggccagtttc cgcatccgta atgaaaacga aagaaaagaa tatgtagggg
     1381 aaatctaagg tattttgacg acaagtcgaa agcataaagc cggagccttg tgaatctgat
     1441 gttcacaagg ctccggcttt gtatagaatc tgtttcgttt tactttctca ggttcggcgt
     1501 gcagtcgatg atgatcgtgt ctgcttgttt cataaccgtg aaattgccct tcgtcgagga
     1561 gaaggctttt tccagcgtat cgctgtgcca tttttggacg gtcatggtcg taccgccaac
     1621 atttttcgaa gacgatgact gtttctgggc atcttccggc aagaggtcgc tgatattcag
     1681 gtcttcgcca tcttttaagg caatggtaac cgtaacggcc cggccgtttt caaagacgac
     1741 ctggtagcgg ccgttatgga agacgcgaat cgtatcaccc tgggaatagg ggtggcccca
     1801 ttcgtcttcc caggcttttt gcgtatcgcc gatggtcggc aaggtggcga ccgccttttc
     1861 tttcgattca gtctggctct tcgtatcctg attggatttc gtatccttcg actgctgctg
     1921 cgtttgggtc tgttcttttt gctgggagtc cgtcggcacg gccgtcgaac atccggcagt
     1981 caaaagggta actaacatga gaacaatagc aagataacgt ttcataataa acctcccgtt
     2041 cattagcggc ggcggaggtt gtctccgccc ttatgacgct gtttgtggcg cttgtttcga
     2101 gttgtttgat aggtgctggc cggtaacttg tcgcgataag tcttctgccg cttcggcggg
     2161 gtgctgctgg cttttacttt gacttgtccc gtcagggtgt acttggtgat cgtggcctgg
     2221 atgttgtgtt caatccggcg gacccaatcg acatcggctc cggtggcgaa catgatggac
     2281 aggccgtcgc tgccggcccg gcccgtgcgg ccgatgcggt ggatgtagta gtcgacgtcg
     2341 tgagggacgt cgaagttaaa gacgtgggta atgccttcga tatcgatgcc gcgggcggcg
     2401 atgtcggtag cgaccagaat ctgggtcttg gccttggcaa agtcgcgcag gacctggttg
     2461 cgtcggcctt gggacatatc gccgtggagt tcgccaatgt tgaagccctt ggcaatcagt
     2521 ttgcccgcta agagggacgt ccgctccttg gtattgcaga agacgatggc aaggtaggga
     2581 ttcatctcgg tcagcatctg gcataatttc ggcagcttct gctcttccgt catcatgtag
     2641 atccgctgtt cgatattttc aagggcaatg gtatcgcctt cgagaatctt caggaccagg
     2701 ggattctgca tgtgtttttt ggcaaggccc ttgatcttat cggggacggt tgccgagaaa
     2761 aataggagct gccggcgttt cggcgtcagg ccgatgaggg tatcgacgtc ttcgaggaac
     2821 cccgactgca gcatctggtc ggcttcgtcg acgatgaccc ggcggacccc gctgaggtca
     2881 agggagttgc ggcggcagtg gtcgaggagc nnnnnnnnnn cgcggctacg tcagcgattt
     2941 ggcgggccaa ttcgcgcgtc ggcgccaaga tgaggacctg ctcggcgtgg acttcggtcc
     3001 ggatctgctg cagcgacggc aagaggaagg ccagggtctt acctgtaccg gtttgagcct
     3061 gagccagtat atcccggcct ttgaacaggg ccgggatggc ttgtgattga acggccgtcg
     3121 gttcgtcgat tccctgcttg tggagaagat cggaaaggtc cgacgtcagg ccgagagatt
     3181 gaaaattttt tgtcatatcg tgccctttct ctataggtgc taacaaagtg cttactaact
     3241 attattatta cacaaagccc ttgtttgcgc aaacttttct ttcctttggg gatatggtat
     3301 actaaagtga tttcgtttgc attgttttag gaggtcttgc ataaatgagt tggtggcgcg
     3361 atattttgcg gcagtctgtt ttcatgtgta tttttatcgt cctcattccc attggagcct
     3421 atacgattca cagtggttcc agtgctattg tggccgtcgt cagttatctt ttcctgtctc
     3481 tcgtcgttcc tacggcctac gtcggagctc aagatgccgt ttttggccgg gaaaggggcc
     3541 gtattcgccg ctgggtcgtc gtcttggtct ggctcttcat gctggccctt aaagtggccg
     3601 tcaaggtcta tctgggcagt tactggaagg cggcgccctt ttgggaatgg ccgaccttcg
     3661 gtcgtgatat cgtctttatt cttgccatgt atgctgaaat gagcctggtc atgctcgtat
     3721 cctatgggct gtcttctttg atgccgggtc gaaaggcggt gggatgatgc gtgttgaatg
     3781 gaaacagaag gggtacgact ggctcagggc tgttctttgg gcggcctgcg gcatcctgcc
     3841 gcttcccttc ggagcctttg cgtcgggaaa gccttatatt gccgccgcgt cgatcctcgt
     3901 cttttttatc ctgtcctttg ccgtcccctt gtggcttggt tatcgccgtc gtaaggcggg
     3961 acgatacgat gactatgcct ctgccggggc gactttctac ggcggtgccg ttgccctcat
     4021 cgtcgccgtg ggcctcttaa acggtctgat cggatcgtct ctttggcact cctattgggg
     4081 aatggtattc ctgttcacct tgtggatgct tttgaccatt gcggctcagt acggggctgc
     4141 gaaaggggtt gatttttggc atgctcggct gcggcgatac tggtattccc agttccttga
     4201 ccccatctta ttcagcctgc cccttccttg tgccgtcctg gggatgttcc tctttccggc
     4261 cttcagcgat gagtcggcct cagtgagcct cttcatcggt attatggcta tcatgggctt
     4321 ttgcttcttt gccatcagca tcttcgtcat cgccaccttt gccttttatt tcttccccta
     4381 taagcgttac ggctacagcc gaaaagagaa gatcgtccat ctcctttcca tcgtcgtcat
     4441 ggtcctcctg tgggtcatgg tccaaaatct cttgttcaat agcgatatgc aggtcctcgg
     4501 atatatcttc aaggccatgc cgattttgca gaataatccc ttagtcttcg tgacgccttt
     4561 tctgttgtcg tcgttggtca tcatcggctg tgtggctctt cgcaatgtcg t
//
LOCUS       DAWDXZ010000093         4568 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4881_length_4568_cov_8.709727, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000093 DAWDXZ010000000
VERSION     DAWDXZ010000093.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4881_length_4568_cov_8.709727"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3225..3234
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1040 a         1252 c         1111 g         1155 t
ORIGIN      
        1 atttctgttc ttccattgtt acctccttga atcttacagg tgataggcac tagtttggaa
       61 aaatcggcga ttcgcccaaa ctacaaccta ccaacctgtg ttctgaaaaa agggccgatg
      121 cagtcacatc agccctacag tatgcgaaat tatttacgca agttcaattt ttcgagaatt
      181 gcacgataac gatcgaggtc gtgtttctgc aagtaaccga gcaaattacg gcgctggccg
      241 acgagtttca acaagccgcg gcgggacgca tggtccttct tgtggctgcg gagatgttcc
      301 gtcaaatagg tgatgcggtt ggtgagcagc gcgatctgta cttccggaga acccgtatcg
      361 ttttcattgg caccaaattc agctacgatt gctttttttt cttctacgct taacattgta
      421 tctttcctcc atgtgtaaaa aattaaaact tattcgcaaa gtgctaagtg atcgtcggag
      481 tcatcgagac accgagcacc cgtacgacat agatattata ctataccctg ccagttgtgt
      541 aaagaatttc ctacataaat cgtgaaaatg tccctcaagt caagaccgtc cccttacaaa
      601 gacgacgacc tcagcggacg gtgtcgcagt ggtccttggt tgcatccgtg aggtagctct
      661 tgatgtcggc cccggtgcat ttgccgaggt cgtcccctgc gtcggaatcg tcaccaagtc
      721 cggcagattc cgaagcgtcg cgttcgacgt ctgcccgttc cggttcgtcc ggccgtaaaa
      781 atacccgggg ttcggacgca ccttcttccg gattgacggc atcatcatcg tcactttcga
      841 cgaaggacgg cgattcccgc aaggccacat cggcggccag tcctgacaga cccgtagcaa
      901 accactgccg attcccggat tggaagacct gctgttgttc atcataagta aggtcgtctt
      961 gtcgtaaatg gtcggccaaa acatctttcc aatcgcgggc atcccagcag ttatcggccc
     1021 cgacggccag atcataagca gtatccaagc gagagtgaag ggccttgcgg atttccctaa
     1081 cggcaatcgg gtcttcgcaa aggaccatca cggccagcgt atcgtcccgg gtgactccgc
     1141 tttcctgctg gaaccactgc caaaacccgt cgaggtcctt cgcagaccgg acatcgtagc
     1201 catattcatc ttgatgccgg tgccaatcga tggaaatccg attagcagcc tcgtcataga
     1261 ccagtgcgcc catttcaatg ttgttccctg tcatgatgaa tatgaatcgt ttcataatat
     1321 ctgtttccct tcttgtttca cagccgactg ccaagcctca aactgtcggt caagccgtgc
     1381 cttcgtttta tgaattttct tctctgccgc ggcaaaatcc gcctcggttc ttttttcttc
     1441 ggactcaaag tgagcctttt gaccgtccat ggccatcttc caaggctccg tctgccgcct
     1501 catcggctcc gaaaggggcc ggcgattcaa ggtcttgggc agcgggcggt tttgtttttg
     1561 aaccatcctt tgccgaaagg cctcatattc cctaccgatt tcgatacctt ttttatcgac
     1621 cttcatccgt gccatttcga ttgcttcttg cagattatgg gccataatat tacctcgtat
     1681 tcccagtata agccgagtcc gactgaaaat caatatcttc gacagcgacg ccccggtcag
     1741 cctggtccat aaccgaatca acccggtcct tatacgtttc gtagacggcc tgatcgtcac
     1801 ggagagcctg acgccattcg tggatgcgca tacggacgtc gtctccggtc ttatcgggat
     1861 agatttcctg ataggccgca atgacggcat cgcgcagttg attgatttta tcgaggctca
     1921 ttccctgagc cttcaagaag tcatcgaaac gaaccgacgt aaatcccagt gccttaaagg
     1981 cattgaccac ggcctgcact tcgccttgcg tcatattggc cctgccgatg gtgacgatga
     2041 agtctgacgg aatctcgatg ccggcgactt tgccccagcc ttcggtgagc cctgccgtcc
     2101 cgaccgtcag atagcgaaga cgattctctt tcagtttgcc atagtcgatg gcttcatcgg
     2161 gaatcatcac tttgagatag gcataatagt catggagatt gtcaaaatct tccagccgtt
     2221 tccaatcggg atgcttcaac tgcccctgtt cgtcgatggc acaagcctct tccagccgat
     2281 agccgatttc ttctacctta tcgtcttcgc taatgatgcc cagtgatttg gcaatcttgg
     2341 cgatgacctt agccgcagct tcaatgatga tttgcgctac aatgggccct aacacaggac
     2401 ctaagggacc gatagcgata gatgccaccg ctccaatgac gcgaacagct gcgccgaata
     2461 tttttcctaa attcccgcca atagccccgg ctacatgagc caaggccctg ccggctgtct
     2521 tgagagcacc gctgacacag cttccgatgg ctcggacggc accggtaaag gcccttccga
     2581 ttcctccgac aagagagcta aaaaatccca tacacctcac cctttcttac aagctatcaa
     2641 atccagtcga tcaagtccaa gcggtcaagg gcctcatatt taccaagctc ttttccgtcg
     2701 aggacatctt gaacccactc atccagcacg attttccacg ctgtcgatgg cttatccgtc
     2761 ggcagtacat ggcagagata cgtcaaaagt atgtctttcc cgcatttttg gccttcgcgc
     2821 cgtatttcgt cgttcaaggc atcgatcttg tcccaggacg attgggaatt ttcctgaagg
     2881 aggctgaaaa tggcatcatt cttcatcaat tccaaataat gttctacatc cgtttttgta
     2941 acctccggca attgttccag ccccggcagg attcccttgc ggtaatgccg ttgatagtag
     3001 ccgacagcga tgtcgtgaat ggccttcggc tgtatttcct gatagcccgt cgtatcggcc
     3061 agtttatcga cctgcacttg cacggccttg gtccaatcct gagaaaagac gacggcccgg
     3121 ccggtaacca agttggataa aaaggctttc tgagtctgat ccagggccat cgtattgccg
     3181 atcgtatcct tatcgtcttg ggcaaagatt ttatggacga tcttnnnnnn nnnnctgatc
     3241 ggcaatgatc agagattcgc cgtactttcg aacttcggcc agcatatccg taaagaccat
     3301 gacgccctgc ttcttgttct gactgtcccc gggctgatag cgactcagga ggcggtgagc
     3361 ttcttcaatc aaggtgatat gctggaacga tttcttcttt tggctggctt caaaatgagc
     3421 ggccttgacg gcctgcagca ggttggtcat gatgaggccc atgatcaggg atttttccga
     3481 agcgttcttg atttcttcca attcgatgac gacgttgcgc ttgatgatat cgcgaaaatc
     3541 aatggatcgc ggcgtgttga acatcatgcc cttggagccg accaacagtc cttgaatata
     3601 agcatggacc gtaccgagat actccgtgaa cagccgttcg tcaaagccct gttcgcggat
     3661 gatggtcggc atgatatcgt aaaagtcctg caaggtcgga aaggcataga cgccgtcggc
     3721 aaagggaccg tccgcatcgt caggaccttt ccccttccat agattggtac cgatgctcca
     3781 gcccttatct tcatagatgc ggtaaatcgc cgcttccaag agctgcggga tggcagcctg
     3841 catatcgaag gtcgcttcaa aggtggcctt aatcatatcg gcccgcgacg taatcgactc
     3901 gccgggaaag agttcaaagg gatttaagaa gaaaggagcc acatcctggg cgccgggcgt
     3961 aaagaaagta atgtccagtt caggatggac gacctgcagc aaccgatatt ccgtcttagc
     4021 cggttcgacg accaagaaag gcaggccgct ttgaaggagg atgttctgac aagtcgtcgt
     4081 cttgccgctg cccgtaacgc cggtaatgaa ggtgtgcatg ctcaggcttt tccgggacag
     4141 catgaccgga atccgttcct gcacgctgcc gcattggacc aatttcccta aaggcaggtt
     4201 gtcgtcgggc cgcttatgtt ggactgcatt gaggccgaat tccacttcct tacggagttt
     4261 caggccgatg acttctttct gcggcacgcc ggccaaaata ctgagctgat cggcagacag
     4321 ccaattgccg cagtacaatc gtccgccggc acaggccttg gaaaaagcac tgtacaaata
     4381 ggacttgccg gcatgacccg taaatcgata gggaatctgt aagttctcca accgattccg
     4441 ataaaaagcg tccgcttcgc caaactcacg gaaggtcaag ggcatgcggt tccccttggg
     4501 gccgctgtat aaggagccga tcgtattggc caggcggtat aaggtcgtcc ttttatcgct
     4561 gcacaaat
//
LOCUS       DAWDXZ010000094         4458 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4976_length_4458_cov_9.934363, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000094 DAWDXZ010000000
VERSION     DAWDXZ010000094.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4976_length_4458_cov_9.934363"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1116 a         1177 c         1157 g         1008 t
ORIGIN      
        1 cagcgccgtc ctcagcggca gcggcactat ggataaaaaa ggcaagaagg atcccggcag
       61 tgtcagtatc agcggcaatg cctcgtggag cggccgcagc ctcgatacgt actccttatc
      121 ggcagatttt gccaatctca atgtggataa tccttactat aatgggcccc ttacggggta
      181 tatcaacgtc acgcccggtg acggacgacc gaaaatcggc ggccttatcg atatcaacaa
      241 cgcgaccatt gacgtcccgc tgaccctcag cgaaagctca tcgacgcctg atttggacct
      301 cgactttacc ttatccttag gcgacaaggt ccgtctttat aactcggctc tctatgacct
      361 catggtgacc ggcgccgttt cctttaaggg cagcctggaa catccgaatc cgtcaggccg
      421 ttttgaagct acgcgcggtt cggttcacta cttggatacg aatttccgcg tttccaaggc
      481 cattgccgac ttttcacgct acgactcact tctgccgtat atcgatgccg aaggtatcag
      541 ccgcgtcggc caatatacgg tcatgctgac cctgcgcggt caggccgata acatggatct
      601 cctgcttcgt tcggatccgc cgctcaccaa gccgcagatc gtatcgctca ttaccctgcg
      661 caatggcagc ggccgccccc agagttccat cggtgaagat gatatgagtt ccctcctcgg
      721 ttcgggcatc cgcatgacgc tgaacagctt gggtattact cagagcctgg aaaaagcctt
      781 gtccttagat atgctgaccg taaccaacgg gtccctcgac ctcaatgata gaaatacgga
      841 tgtcggcaac aactactaca atattgaaat gggcaaatac ctgttcaatg actttatggt
      901 cacagcggcc tttggcctca atcacgatga caatcgtttc ggtatcatgt atgatttggg
      961 cagccgcttc agtctcagtg cctggacatc gagcgacaac aactttatcg gtgccctcta
     1021 taaatacaat ttctaaatct atctttatta tgtgcctcat ggaaacgatg aaaggaggtg
     1081 aaaggccatg ctggaagatt atttacagga actgcagaag ataaccttgt tgcagccgga
     1141 agaagaagct gctttgtggc aggcttataa agaaggcggc gatgccgggg cccgcagccg
     1201 tcttatcgaa cagtaccagc cactggtatt taaagaagcc atgcgctgga atatccacac
     1261 ggatcttttg ctcgatgccc tgcaagaagg gacactgggg ctcatggaag ccgttgagcg
     1321 ctatgaccac agccgcggtg tggcctttcc cttatttgct gttcatcgca tccgcggggc
     1381 tatcctcgac tatttaaagc gggaaggggc cgtcagtgcc atgtccctcg atgcaccgga
     1441 cgaacagggg atgaccctgc aggatacctt gtgcgatgac agagaggatc cggtggaagc
     1501 ggcgagtcgc cagttccttt ttgaaaaagt cagcggggta ttgcagcgcc tgcctgaaaa
     1561 agagcaggcc gtcgtcgaag gcgtctatct gaaagatgtc gagcagaagc atttagcgaa
     1621 ggccctcgat atcagtctgc cttacgtata ccgtctgcaa aagcggggca tccgcagggt
     1681 gagggggatg ctcagccgct ttattcacga cagccgggat taaatttttt gtaatttcct
     1741 aaatttggcc tgtttttagg tgaaaagaat tggaaaatgc tgtaaaatat agattatggc
     1801 atatttttgc tttcttttgc cggtataaac tcatatggcc tcatccgata ttataagcgt
     1861 attcgtttat acagaaagga gaaggctgta tgaaacgctc ccaaaacgat acccttcacc
     1921 gtgttcgggc tgttcagtcc tggctggaaa aggcggaagc atcttttgac aaagagtccg
     1981 atatcaaggg cgaactcaat ttgatgctgg ccgaggcgga aatgaagaat ttacgccgac
     2041 accgtcccgc ccataagcgc tttttgcgcc ttggagccat cgtcacggcc ctttgtttgg
     2101 tgacgggcgg ctggtatctt ttccatcagc cgcggcagac tgacttgccg cggtcggtag
     2161 aagcatccgt ttatggagaa cggccaaagc atgaatcagg cgatactgcg gacagcgcag
     2221 ccccgccggc tgcagtttcc aaagaagctg ccggaaagtc cggcacggat caggaaacgc
     2281 agctctctgt tcaatctttg ccgcaaacgt cgatggcagc aactcctgct gttgtcgaag
     2341 aactggctga acagccgtcc gctccggctg caggcgacca gcaaacggca tcgcccgctg
     2401 tgcgggaaga aaaggcagtc ctgtcggaca gccaattaca agctactgta caagaagcgc
     2461 gtcataccct acgcggtacc ggtatggcga aataactaat ggaggatttt tatgaatatg
     2521 cggaagaaga aactgctcgc gctgatggta accagcgcga taatgacttg ccccgtctgg
     2581 gcagaggata cgacggcgac gaccgggtat gaagcgtcga ctaccggaac ggttacgact
     2641 tcaggctcgg cggcagcacc gacggatgat gacaagaaaa cagcggtcaa tgctgtcgta
     2701 acttcgccga atgatgagtc taaagaagaa gcaatccaag ctgctaaatc aggccttacg
     2761 ggttatgaaa cggaagatga actggcttcc attgcagccg ggagaagtcc tgaagccgtt
     2821 cagcaggaag cggaagaagc ggctcgcaag gctgaagaag ccagaaatcc cgaacctgtc
     2881 gatacgacac cgcagatttc cggtgaaaaa gttacttata tgagcgggac cagtgatatg
     2941 cagttcgata cttccattcc cggatatccc atggcggtca tccaacccgg acaggacgta
     3001 tccctgccct acggtgaagc cgtaaccgat gccgaactga agccttatgt cgataaggtc
     3061 gcaacgtctg tttcggtcag ccccgtaccg gaaccgcaga ttgaacagaa catcctgcct
     3121 cgtctggcca tgcgcgtcgg cgatgccatc aacatggact acattcgtca tgacctcaat
     3181 gtcatcgggg cgacgggttt attttcaacg gttaaaccgg cctttaccaa cgtccctgaa
     3241 ggcgttgtcc tgaactatac cgttcaaatg aatcctgtcg ttaagaaaat cgacatcgtc
     3301 ggcaacgaat cgatcagcac cggcgatttg atgaaacttg tcgctacgac gccgggcacg
     3361 accctcaata cggccgtcgt cagccatgac gttgccaata tcaatacggc ctttgccaat
     3421 gccggctata tgatgagtca cgtatctgat gtccgtttgg acgacgaagg gaccctccat
     3481 attgcgatta ccgaagcccg cattgaaaat atcaacctcc gcggcaatac gaagactcgg
     3541 aataacgtca tcatgcgtga actgcgcatg aagaagggcg atatcttcaa taaaaatgcg
     3601 gctagccgca gtattcagcg tgtttataat acgggctatt ttgaagacgt caacgtccgc
     3661 ctcctccagg gccagaaaaa tgcccagaac gtcatcgttg aaatcgacgt taccgaacag
     3721 aagacggggt ccgttaccat cggtgccggc tactccgatt cggacggctt agtcggtatt
     3781 ctcggcctca gtgaaacgaa ccttcgcggt accggtgata aggtcaacat cagctgggaa
     3841 tttggcggca acaccgattc caataagaac tacatcttct cctatactca tccctacctc
     3901 aatgatgccg gagactccat cggcttcagc ctctttgacc gcgaatcgga atacgaagac
     3961 tataatgaaa agggcgactc cgttgccgac tatgaccgcc ggaccaacgg cttcaacgtt
     4021 acctacggcc gtgtccgcag tgaatacgtc agcgactatg tcaccttaga aacgaagaaa
     4081 acgaaatata ccgactataa tagcggggct gactatcagt cctgggttga tgggacgggc
     4141 gactactatg gttattgggg cggcagtgcc gatgcctatc gcaattattt aaagaataac
     4201 ttcggccgga ccaatagcat gacctggtct cacgttttcg ataaccgcga taacgtctac
     4261 gatccgacga aggggaaacg cttggccttc accggtgttt gggccggtca cggcatgggc
     4321 ggtgacttcg actacttcaa gttcattgca gaaaaccgcc tctattataa agtcggccgg
     4381 gctcacgtca ttgccgttcg tctcatgggc ggtattgcca ccggcgacat gccctacaac
     4441 gacctcttta ccttgggc
//
LOCUS       DAWDXZ010000095         4454 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_4983_length_4454_cov_8.693794, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000095 DAWDXZ010000000
VERSION     DAWDXZ010000095.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4983_length_4454_cov_8.693794"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    399..408
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1111 a         1135 c         1109 g         1089 t
ORIGIN      
        1 aagccaagtc ttgcgacgtt gaacgatacc gttccccacc ttttgcagga ccgataaacc
       61 cctatttttt atcgccatac ccctgcggat ggtgttgatg ccaatgccag gcatcggcta
      121 taatttgttc tactttactg tgtgtcggct gccagcctgt gatgcggcgg attttttccg
      181 atccggcaat gagcacagcc ggatcgccgg cacgacgcgg tgcttcttcc acgttaaaat
      241 cacgacctgt aacctttttg gcggcttcaa tcatttcccg gacgctgaag ccgttctgag
      301 agcccaaatt aaagtactgc gactcgccgc cttccgacaa gtagcgaagg acgcgtacgt
      361 gggcatccgc cagatccgtg acgtggatat aatcacgann nnnnnnnnat cacgaacaca
      421 agtcccgtcc ggcgtcggat agtctgtgcc gaaaatttta atgctgtcac gctgtcccaa
      481 agcggtctgc aaaatcaaag gaatgaggtg cgtttccggt gtatgatctt ccccgatggc
      541 accactgcca tcagctcctg cagcattaaa gtagcgaaga gctacatagt gcagaccgta
      601 agcacggctg aactgagcca acatcatttc aatcatgagc ttggtctggc catagacatt
      661 ggtcggctgg tagggcatat cttccgtaat cggcgtcttt tccggttcgc cataaacagc
      721 agccgtcgac gaaaagacga agtatttgac cttggcctga cggacagctt caaggaggca
      781 atatgagcct acgacattat tatcgtaata aatggtcgga ttttccatcg attcgccaac
      841 ctggctatgg gccgcaaaat gcatgacggc atcgatgttc atgtcttcaa gaatccggcg
      901 tacgccatca atatcgtgga tgtcgacgac gtaaaaggga atctcttccg ggacggctgc
      961 ccgatggccg cgggacaggt tgtcaatgac gacgacctta tggccttctt gaagcagggc
     1021 cttcaccgta tggctgccga tatagccggc accgccggta actaatacat tcatagctta
     1081 accttcctgc agttttttca ggcggccgcc gaggaatttc ttgtatactt cgacgccggg
     1141 ctgattgaag gcatcgacgt tggcaaattc gccttcataa gcaatggaca ggcagagcat
     1201 gaacatcaat tcgcccaaat gatacggagt gatggccggc atgatgtagt tggcactggg
     1261 acggccgtca ccggcaaggg cttcagcatt agagctgcga gcagcttcta agatctgact
     1321 gagaggcaaa ccgctgaaag cggccaattt cggatattca tcgtacagat gaggtaccgt
     1381 aacgtcatcg ggccagtttt cgacagagac gaactggacg actttatcct gcgggccttc
     1441 ctgatgttcc tgggtctgcg catgcatgtc cgtcgtaccg acagccacga tcggcgtacg
     1501 gccatagttg acgacattgc cctgtttgtc aaggcgcttg ccgagggatt cagccaagag
     1561 ctgaatatac cattctgaca aggatttcaa gctgtcggca tagggcatga gaacttccag
     1621 gctgcggcca tgatttttac cggccaggaa tttaaggaca ctgttgagaa gggccggatt
     1681 tttccaaata tccggattct ggcaagcgtg atccatatca cgggcaccgt caaggaactg
     1741 acggatgctg aaaccggtca aggcgcctac gatgagaccg acttcagaaa agacactgaa
     1801 tcgtccgcca atgccatcgg gaacgtagaa aataggccag cccgattttt tagccagacc
     1861 gtgaagcagc gtttctttat cgccgccgtc gtgagggtcc gtaacggcta cggtttccag
     1921 ggcaatgccg gcgtcgctca tagcttcttt caagaccatg tagttcgaca tcggttcgat
     1981 tgtcgagccc gatttagaaa tgacaacggc cattaccgta tagttcggct tcgtctggct
     2041 gtcatttttc aagaattgaa taaggcctgc cattttatga gagtcaacat tattgccggc
     2101 aaagaaggct ttcgggaagc cattgcgttc ttccgtgctc tttaaattcc aaaattcacc
     2161 gcactgaacg tcaaacaaaa ctttcccgcc caaataggaa ccgccaatcc ctaagaatac
     2221 gacggcatcg acgcggttgc gcgtcgtccg agccaattct tcaagggcaa aaatacgttc
     2281 cgggctgttg atgttgccat cttcaatata cggcagctgc gtaaaaagaa cggcttccgg
     2341 ttctccgtct ttggagagat gtcccggcat ggtgccgttc ttgcgcatgt tcatcaccgc
     2401 ttcatgagcc ttggtataga ccggtgaaaa gtcctttacc tctgcttccg tgacggcctg
     2461 agggccataa agattggtta cgtcaaaagt aaagcctgaa cgtaatttta actgtgtcat
     2521 gttcacttca ctacctttcg atagaataat gtcttattca cgtatgaaat cattatagca
     2581 cagaacgggt atactatgcc acctgcgggg cactgaatgc cccatgtaaa ccaaaagccc
     2641 ctgtacgggg ctttaaagac agcacgatgt tctgcttaga accgcagcat cgattgaagg
     2701 aagctttgtg tccgttcatt ttgcggatgt tcaaagacgt cttccggttt accttgttct
     2761 tgaataatgc cgtcagccat aaaaataacc cggtcggcca cttctttggc aaaagccatt
     2821 tcatgggtta cgatgatcat ggtcatgttt tcatcggcca actgctgtat cgtgcgcaga
     2881 acttcaccgg tcaattccgg atcaagggca ctggtcggtt cgtcaaagag catgatatcc
     2941 gggtccatcg ccaaagagcg ggcaatggcc acacgctgct gctgaccgcc ggaaaggcgt
     3001 gacggatagg tatctctttt ttcccacaag ccgacctttt taagcagttc ctcggcctga
     3061 ggcatgattt cttcggtctt catcttcttt acgacgcgag gcgcttccaa gatgttatcc
     3121 aacaccgtca tatgcggaaa caagttgaag tgctgaaagc acatgcccat gcggcgacag
     3181 atagaccgaa cgtcggcagc cggcgcatag acggcacgct ggctcccggc aggcgtactg
     3241 gccatggtct taccgtcgac gacgatagag ccgctgttga tggtttccaa ctggcaaagg
     3301 catctcagaa aggtactctt accgcttcct gacgggccga tgatagaaac gacttccccc
     3361 gcttctacat ggagggagac gtcttttaat acttccaaat tcccgaaact ctttcggata
     3421 tggtccattt caataaaact catgacaaga cgtcctcctt attcatcata gacggcgtag
     3481 cgtttttcca gataaccgaa aattttggtc aggataaacg taaagaataa atagaataca
     3541 gcagcaacta aaaaggccgt cgtatcgaaa tcgcgctgaa cgatggaccg cgtaatgcgc
     3601 aggatgtcgt tcatggccag gatgtagacg agggacgtat ctttgagcag gttaatcgtt
     3661 tcattggaca ctggcggcaa aacccgtttt acgacctgag gcaggataat gcggcgcatg
     3721 gtctgaatat aggtcatccc caggactttg gctccttcat actggccgcg gtcgatggac
     3781 tggatgccgc cgcggaagat ttcggcaaag taagcggcat agttaaagac gaaggcgatg
     3841 atggccgccg gaaaggccgg cagggtaaag ccgtcgatga taaagggcag tccgtaatag
     3901 atgaacaaga tctgcagcat cagcggcgta ccacgcatga cataaatata ggcatacatg
     3961 gtctttcgca agatggctat cttagaaatg cggccaacgg ccatgaggat acccaagggc
     4021 agactcaaga cgatggtaat aacaaaaatt tgcagtgtaa ttttcaggcc tgctgcgacg
     4081 gcaggcacaa tctgaattaa ataatccatg ctggctcctt tttcaggcac ggcgattcac
     4141 acaggcagtg tgcaacacgg aaaaacaacc tctccgtcca tgccggggac ctctccacga
     4201 cggggagctg tcagcgtcat ctgaccgaaa ggttgttttt cgtacatctt tcccgattac
     4261 cgtactttta tggtaatatc ttcgttaaac cacttctggg acaacttagt cattgtacca
     4321 tcttcactca tctgtttcaa gacatcattg agtttatccc gcagcagatt gtcatcttta
     4381 cgcaggccga cgccgaattg ttcgtcaccc attttgtcat caatgacttt aaactgaccc
     4441 ggcttcttgg tcat
//
LOCUS       DAWDXZ010000096         4404 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5034_length_4404_cov_5.958841, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000096 DAWDXZ010000000
VERSION     DAWDXZ010000096.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5034_length_4404_cov_5.958841"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1746..1755
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2972..2981
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3892..3901
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1078 a         1010 c         1161 g         1125 t
ORIGIN      
        1 ttcagacgga tatggaagaa taaagagggg aaaaaggtga agttccaact taaggactat
       61 ccatggatga aggccctgca aaattggtgg cagcgagtaa agccgacgaa gatgccggct
      121 tggtggcctg cctctttgca ggttgaccgg accgaaccat tggtggtaac gccggagcag
      181 tataaaaaat tcattcgaat cggagtcgcg acagcaattt tcattgtaat tgctgtcggc
      241 atcggtattt atgaattcgt ttcgctccaa caggctaaag accaagaaat gctgcatgaa
      301 cagcagctgg agctgatgcg ggataagacg acatcgctcc aagaaaaaat ggataaaatg
      361 gatgcactcg accaggaact gcggcaaatg gtcaagggat cgggggctgg tgacggtggc
      421 gtagcagggg gacagaagaa atctccggac ctttcgaatg ccagctacaa tgacctgtta
      481 ttggccacct ctcgtttgga aacgcgcagc aatgcccgta ttatcagctt tatcacctta
      541 aagaccgtcc ttagcgatac ggccggtcaa caagtactgc agatgcagca aaacagccgt
      601 atgtaccagg tcggcggcaa ttcaggcagt cccaacagcg catctaccgt gccgtctatt
      661 tggccggtca cagggaccgt tacgtctgaa ttcgggtatc gagggaatcc tattggcggt
      721 ggttccggct tccatgaagg gatcgatatc ggcgttgact acggtgtccc cgttcgggca
      781 acggcagccg gcaaggttac catggccggc tgggtcgatg gctacggcaa cctcgttgaa
      841 atcgatcatg gcaacggttt tgttacccgc tatggtcata attccatgct cctcgttaca
      901 gtcggacagg aagtccaggc cggatctatt atttcactgg ccggcagcac cggccggagt
      961 acgggacctc acgtccacta tgaggtccgg gtcaatggat cgccgacgaa ccccttgctg
     1021 tttttgcctt gataaaaaaa ctttggcaga ctttttttca aatgaggaag gaggactttc
     1081 atgaaaaaaa atgttcagtt ccttgtccta tttgtctttt tgtctgcctt tttgctgggc
     1141 ggatgtgcct ttgttcgcca gtatacaggc gttctcatcg gaaccaatct ggctctgagc
     1201 ggaaaaggga tgtcctattc ggcgtcaacc gaacgaggca tcgaattggc gaaggatatt
     1261 gtcaatgaac gcggcggcct gctgggcaaa ccggtacagc tggtatcggt agataatcac
     1321 ggaaagcccg aagatgccgt ggctgcgatt cagcagctga acgtacgaca cgtgtcggct
     1381 atcatcggtc ctgatataac cgactgtacg agagccgtca ttccaaatgt ggaagccgtt
     1441 aagattcccc ttatcagtcc ggccgggaca cagcccgata ttactgtcga tattcgggcc
     1501 aatgaagtct atcaatacat gttccgagca gcctttatcg acgcctctca aggtcgggct
     1561 atggtagatt atgcccgcca taaccttcat gccggcaggg cggctgtctt ttactatcct
     1621 gataagatgt atgcgcaagg actggcagaa tactttcgca gcgcttttct tgccggcgga
     1681 ggcgaggtcc ctatctttcg agctgtatcg gatccgggag aactcgttca ggttgtatct
     1741 gaattnnnnn nnnnncgacg gcgtcgatgt catttatctg ccgggatacg acgattggac
     1801 gattccggct attaaagtat tgagaagtca aggaatctcc gtgccgttgt tggggcctga
     1861 cggctggaac agccttaaaa tggaaaggga tattgagcct caatatctga cgaatcttta
     1921 ttataccgat cactatgccg gaaacgagtc gagtgaggct gctcgtcagt tttctcaagc
     1981 ctattatgaa aaatacggcg tttggcccga tggctatgca gcccttggct acgatgcctt
     2041 tatgatgacg gcagccgcca tttcacgctg tcagtccggg gatccgacag aggtcgctaa
     2101 agaattggtc aagacgattg actaccaagg ggcgaccggg acgatttccc ttgacgccaa
     2161 tcacgatgcg attaaagatg tgttcatcct gactttcaaa gacggagagc cggaacttgc
     2221 agcgacgatt ccacctgatt atcagatgca gtagggcaga tagcggttca tggccttgcg
     2281 gcgctccttc cagtccggca tgcggacggt catgacgtca taaaaatggc gggaatggtt
     2341 ggggtggatg aagtggcaga gctcatggag catgacgtgg tcaatgatgg cttccggcat
     2401 aatgaccaag taggtgttca tggtcacgat gccttttagg ggcatgcacg agccccagcg
     2461 agagcgcatg attcgttgtt tgaggaccgg ttctttgagg ttatattctt taaataaggg
     2521 cagcattcgt tggagactgt ctgaaaaaag gggtgctccg atgcgctgca aaagctgatg
     2581 atacagtttc tgcctcgtct gcggcgtatc ttccttgagt tccataaaca ccgtgtcctg
     2641 gtttcgccgg atggaattgc gaaggcctag acggacgtcg agacggtaag gcttgcctgt
     2701 aatcaagagg gtttcgccgt cactcagtgt caggaaggga gaatttttct gttgttcctg
     2761 catcttttgg aaggctcggc agataaggtc gctctttttt tgtaagaagg cttcgatata
     2821 ctcccagggc acacggggcg atgcggaaat gaacagggat ccgtcgcctc ggatatggag
     2881 attgatgttc tttacttttt tgcggacgac cgttacggaa aatgtccggt cctgagccgc
     2941 aaaagtatat tgttcactgg tcatagaaat annnnnnnnn ntcgtacaga taacgtaatc
     3001 agaatgcaga ttttattgta agtagatttt agatttttgt caaaaggatg gtatcgtatg
     3061 cttgatttta cggcatttga ttttgaaacg gccaataaat acgccaacag tgcctgctca
     3121 ttggcggcgg tcaccatgaa tgacgatcag tgtgtccgcc agggctatac gctgattcgg
     3181 ccgcccttta tggtattcga cccgggaaac attgaaattc acgggattca gccggaacaa
     3241 gtcgcccata aaccgaaatt cgatgccttg tgggggaata tccgaaccca tttagaaggg
     3301 cggattttgg tggctcatta cgccgttttt gatacgcgcg tcctgcgcag tctcctgaag
     3361 tattaccatt tagaaaaacc acaggcttcg tatgcctgca cggtagaaat atcgcgccgc
     3421 gtgtggccgg atctgcccaa ccataaactc gatacggttg ccggttatct gggctattct
     3481 ttccagcacc atcaagccct tgatgatgcc agagcctgtg ctcacatcgt cttaaaagcg
     3541 gccgaagccg taggcgcttc gtcgatggaa gaattgttgt gggagacgaa tttaaaatta
     3601 aaacagctat aatttactgt tggcagtcct tcacgattgc cggcagtatt tttttcctta
     3661 tttttgatgt atgctatctg ttttttgtat agcattacat tttatatgct tattttccat
     3721 agaggattac aaatccctac tatctgcgta taattaagac agtgattact atcctgaaag
     3781 gagcttttta acatgaaaat cgtcttatta gaatcgttag gtatcagtca ggaattactt
     3841 gactcttatg taaaaccgct tacggaaaaa ggctatgaat ttgcagccta tnnnnnnnnn
     3901 ntgccgatat cctcattatt gccaatatgc cccttaaggc agaggtcatc aacgcctgcc
     3961 cgaacttgaa atacatcgat gtcgctttta ccggtgtcga tcacgtggcc ctcgatgcgg
     4021 ctaaagcaag aggcataaaa gtcagcaacg cgtcgggcta ttcgaccgtt gccgttgcag
     4081 aactgaccat tgccatgatg cttgatttac tgcgccgcgt tcctcaagtc gacgcagcct
     4141 gccgggccgg aggcaccaaa gcagggcttg tcggcaacga attggaaggg aaaaccgtcg
     4201 gccttatcgg gaccggtcat atcggccatc gcgtagccga attggtccat gccttcggtg
     4261 ctaaggtcat cgcttataat ggtttctccc ataaagaaga tacggaatta atcaagtatt
     4321 tgccgttgaa agaattgatg gcccagtctg acatcgtcag ccttcattgc ccggtaacgg
     4381 aacagtctaa gggcatgatt aacg
//
LOCUS       DAWDXZ010000097         4350 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5073_length_4350_cov_10.699884, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000097 DAWDXZ010000000
VERSION     DAWDXZ010000097.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5073_length_4350_cov_10.699884"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          981 a         1022 c         1266 g         1081 t
ORIGIN      
        1 tcggtgattc gacgcagaca catgaagggg cgacggccga cacggctcgc gtcgttgctc
       61 gagccggggc gtctgtcttg gttacatcga aaacttcagc tgaagataat ccttatggga
      121 tggatgaagc tacggggaag ggaaatcacg cagctaacct taaaggcgat tatgatgtgg
      181 cccgcgtcgt tgttcacaat gcccgcagtg atttcggctt agatacgtat gcgtcgaaga
      241 ccggtacggc tatcggggct tatgccaagg ctaacggagt ccagtcagcc gcgcttggat
      301 ataaagccga ggtcatcaga gcggctgaaa atggattggc cttgggctat gccagccaga
      361 cttcggccaa gaacagtgtc gctctcggtg ctaacagcgt agctgatgaa gaaaatacgg
      421 tttctgtcgg atctgacctt ttgacgcggc gaattacgca cgttgccgat gggacggctg
      481 ccactgatgc tgctaccgta ggccagctcc agtcggcttc cgcctccctt catcaaaata
      541 ttcgctcttt agggacggaa attgatcagg tcggcgctgt ctcggcagct cttgccgggc
      601 tgcacccgct ccagtccgat aagggctttg aaatctctgc tgccggcggt gcctatgacg
      661 ggaaacaagc catggcctta ggcggcttct accacgctaa tccggatctc ctgttttcct
      721 tcggagctgc cacttctttt gatggtgacc ataagaccgc cggtaacgta ggcgtcactt
      781 tccgcgtcgg cccgaaagcc gaaaaaaata catccgtcga tatccgtacc tctgatgaag
      841 tcttgcagcg cttagacgcc ttgagccgaa aagttgacaa gttggaagca gaaaatcaag
      901 acttaaagaa taaattgaac ataaagtaat gcagatcgcg gatgaaataa ttatttgaca
      961 acgatattga gacgaatgcc aaaagcctgc cgtttgaacg gcaggctttt tgttccttgt
     1021 taacgatggc ggcgtcatct agctcatgca tcttgagtga atctgcgttt tctttgcgat
     1081 tgccttcagc agttttctac gatggtcagg gccttgagag cacttctttg ttataggccc
     1141 ataaaggcca gcatctgcat catccccggc aggaaggtat cgagatcgag ggactcttct
     1201 aaggtgtgtt cacggcgact gcggcagaag ccgaaggaaa tggccgggat cccctgactc
     1261 aggggaatat tggcatcggt acttccggca atgcaggttg tggtcaggcc ctgctgttta
     1321 cggatggccg taatcctccg gacgagggga ttctggtcga cttgactgca cggccgttcg
     1381 ccgatgcttt ttaaggaaat gcgaacatca cgggacggct tttctaagag ggcgtggaag
     1441 gccttgtcga ggctgtcaag agacgttccg tcttccgaac gaagatcgac ggtgaagcga
     1501 acctttgcgg caatggaatt gaccgtcgtg ccgccgctga tggtgccgac gttataagtc
     1561 gtcttgggtt cggccgggag cgagaggctg taaagcgaat cgatgacgga tgcggccgcg
     1621 gtgatggcat tggcgtttcc aaagcccatc cagctgtggc cgcccggcgt ttcgatggtg
     1681 acctcatagc gccgggagcc gacggccgtg gcgacgaagg tgtcgaaggt gcagtcgacg
     1741 gcgacgactt cagaaatgcg gccggcccag tcggtcatga tttggcgaat gcccttgaga
     1801 ttgcccaagc cttcttcgcc gacgtcaaag gcgaagagga taccctgagg cggtgtcagc
     1861 tttaagtcga aaatcatctt gatgatgaat aacaggccgg caatgctggc gctgttgtca
     1921 ctgcacgacg gggccgacaa gatatttttt gattggtgaa tcggtatctc ggacaacttt
     1981 tcaaagaccg tatccatgtg ggccgtatac aaggctgctt tttggccctt tttcagccgg
     2041 cagggataga ggatgttgcc gctgtcatca cggtaggctc cgtcggcgcc cagactgtgg
     2101 aggtactgta agatccattt gcctttgact tgttcccggc ctgtcggtga cgggatggaa
     2161 ataatcgttt tgaggagctc cagccactgg tcttggttcc ggtgtacata ggtttcaatg
     2221 atgtcgttca tgaatgtcat ctcccgtaag aattttcctt attataccat atctatggga
     2281 atgatgattt ttcgtattcc tgtattttcc ataaggtaga cgcaaaaagg ccctgtccgc
     2341 atgggacagg gccttggtgt atattatcct aagatttttt tcaaatcggc ttccggcgtc
     2401 gtcgtcgggg agatgttgaa gttttcaact aagtagttga ggacgttcgg cgagaggaag
     2461 gccggaactg tcgggccgag gtagatgttt ttcaggccta agtagagaag ggtcaagagg
     2521 atgcagacgg ctttctgttc gtaccacgag aggaccaagg tcaagggcag gtcgttgacg
     2581 ccgcagtcaa aggctttcgc caaggcgacg gcgacctgga tagcgctgta tgcgtcgttg
     2641 cactggccca tatccatgat gcgcggcaag ccgccgatgg tgccgagatc gaggtcgttg
     2701 aagcggtatt tgccgcaggc cagggtcagg atgacggtat cggccggggt ctgctgaacg
     2761 aagtcggtgt agtagttacg gcccgggcga gcgccgtcgc agccgccgac gaggaagaag
     2821 tgtttgatgg cgccggcttt gacggcttcg atgactttgt cggcaacgga cagaaccgtg
     2881 ccatggccga agcccgtcgt aaccgtcgta ccgccgttga tgccggtgaa gtgctgttct
     2941 tctttatagc cgccgagttc caaggctttt tcaatgaccg gcgtgaagtc cttgtcttcg
     3001 ccgatatggg ttacgccgtc aaaggagacg acttccgtcg tgaagacgcg gtcgatgtag
     3061 gatttcttgg ggggcatgag gcagttcgtc gtgaacagga aggcgccggg gacgccgtcg
     3121 aattctttct gctggttctg ccaagccgtg ccgaagttgc ctttgaggtg tttgtatttt
     3181 ttcagttccg gatatgcatg ggacgggagc atttcgccgt gggtgtagac gttgatgcct
     3241 ttgccttctg tctgctggag gaggcgttcc atatctttaa ggtcgtggcc ggtaacgacg
     3301 atgaacgggc ctttttcgat ggtcaaggga accgtcgtcg gaaccggtgt gccgtacgtc
     3361 gaggtgttgg cttcgtcgag gagggccatg caggtcaggt tgactttacg ataggaagca
     3421 gttcgtccat tccccagttt tcgctcaggg ccgaaaggcc tttgatgaag aaggcgccca
     3481 gttcgtcact ggatttgccg agcatcaggg catgataggc gtaggctgcc atgccgcgca
     3541 tgccgaagag aatgagtgat ttcaaggagc gaatgtcttc atcggcattc cacaggccgc
     3601 tcatgtcgta gtcgtcagcc ggggaatccg aaagagaggc cgattcagca tggactttgt
     3661 cgatcataac ctgcgtcgtt tcggtgttga agttgacgtt ggtaacggtc gtgaacaggc
     3721 cttcgagaat gacccggtag gtctgcgccg tcggaacggc ggtctgacag gtacgggcga
     3781 gaccgatcag agcgccggtc aatttgtcct gaaggccggc ttcagccgac gattttccac
     3841 agacaccggc accacccgta cagcctgtac cgaaagcggt ctgttcacac tggaagcaaa
     3901 acatctgttg ttcttttgtc attgaaaaaa acctcctcta tctttatcac aataaaaaat
     3961 gaatgaatac gtaagttatc tagtatgtct tcagctctta tcgagtatac gtagagtata
     4021 gcaggagtcg atgtggattt ccgttgcatt tacaacaaaa tgaaaattat ctttcctttt
     4081 ggaaacgaaa acgatgcttg cgaaatgagg ttttctacgg taatatagat gtatataatc
     4141 tatattataa tagattcgac agatatgtaa ataggaggaa ttcacgatgt cagataataa
     4201 aacctatgct gtgtttaaaa cgaataacgg cgactttacg gtagagctct ttgcagaaaa
     4261 agcaccgatt accgttgaaa attttgtaac cttggccaag gacggatttt acgatggaac
     4321 gattttccat cgcatcattg ccgacttcat
//
LOCUS       DAWDXZ010000098         4332 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5094_length_4332_cov_9.072481, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000098 DAWDXZ010000000
VERSION     DAWDXZ010000098.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5094_length_4332_cov_9.072481"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1759..1768
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2222..2231
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3496..3567
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1110 a         1111 c          983 g         1036 t
ORIGIN      
        1 cgactgcctt cccaactgcc cgaccggggc catcaccttc gtcgaacgcg aagccgctga
       61 ctacgacgaa gaagccgtca aaaagaacat ggccgcccag ggccgtatct ataaagggcg
      121 ctgccccgga agcgaagctc atgccgtaac cccggaagct gcaaaagaat cccgtcaagg
      181 agaggcttca caactccgtc aatggccggt ccagctcaaa ctcgtcccgc ctcatgcccc
      241 ttactttaac ggcgcccggc tcttgattgc tgccgactgc acagcctatg cttatgcccg
      301 tttccacgac gattttatca agggccatat caccctcatc ggctgtccca agctcgatga
      361 catcgactat tccgaaaaac tgacggaaat cattcgccag aacgccattc aagatgtcac
      421 tgtcgtccgc atggaagtcc cctgctgccg cggcctggaa atggccgtaa aaaaagctct
      481 ggaccagagc ggtaaatccc ttgaccttca ggtcaaagtc atcagtcccg acggaaggat
      541 aacggcctga ccgataacgc tatatagaga caaaaagatg ctgccgcaag tgcgacagca
      601 tcttttttgt taaacgttcc attcttcttt cagtgtggat aaacacatga acgtctgcga
      661 agaagcgacc gtcggaattt cattcagtac ctgcatgagg aaatgttcca aatcatccgg
      721 tgtctttacg agcactttta acaagtaatc gaaggcgccg acgacgtgat ggcattccaa
      781 cacttcagaa tactgaacga ttttcgcacg gaaatcctcg tcatcgcccg accgttccag
      841 gttgaccata atgaaggcgg tcacgttgta tcccgtcttg cggcggttta tcttcgcacc
      901 gtagccttca atgatgccgt tgtgttccat cttacgaatt cgttcggcaa cggcgggaat
      961 ggacaagccg acaactttac ttatttctgt tgccgatgct tttccatttc gtttcaaaat
     1021 cattaaaatc ttgtcatcaa tatgatccat ctgccaatac taccctccat agctttaaaa
     1081 tctattatac ctatattatc ttacaaagat cgcaaaaagt aaatagactt aaagttatta
     1141 attatttatt cactatactt tttacttcta tggaagtctt tttattctga aatatgaaaa
     1201 tcagcattta gatcagagaa atgatctcgg aatccgggtg tcatataaat ggcattatcc
     1261 gttccggtaa tttcacagtc cacaccttcc atactgtgga ccaaatcgag gcctttctgg
     1321 ggccccaaaa cgaagacgat ggtcgtcagc atatcactga gcatgccggc atggggcacg
     1381 gacccatcga taatgataga gtcgctcatg acaccggacc gagccggata acccgtacga
     1441 gggtcaaaga tgtgatggta gcggacaccg tcttggatga agtagcgttc ataatcaccg
     1501 gacgtggaca aggcctggtt ttcaatggcg acgatcccca aaaagtcctg gttattatcc
     1561 gaccggggat gcttgatacc gatgttccac ggctttccct tggtcgtctt gccgacagca
     1621 tacatcgagc tggctccgag gttgatgagg ccgctttcaa tgtgatgctt ttcgtaaatc
     1681 ttgcggattt catcgacggc atatcccttg gcgacaccgc ctaaatcgat ggccatcccc
     1741 ggttttgcca gcatgacgnn nnnnnnnncc gcaggaggat atctttataa tttaccttca
     1801 tcaaggcctg ggagacctct tccggcgacg gcacatgctg gtcggcattg ccgatatgcc
     1861 acaagttggt catgaccccg atggtgatat cccattcgcc ctgactcttg gccgaatagt
     1921 ccttggcgta ttcaatcatt tcataaatag ccggatctac ctttacatag tccttaccgg
     1981 cagcggcatt gatgcggctg acatcgctgt cttcattttg accggccaat ttatctaaga
     2041 cagccagccg ctcgaagcct tcatcaacgg cttctttcgc ctcctccccg gtcgcccgga
     2101 gagagaccga cgtatccatg gcgacgccgc tcctatcaaa agaagactcc ggatgcaagg
     2161 tgcatccgga gctgcttaat acgatgagga acgccatggt gagaagaacc catacatacc
     2221 gnnnnnnnnn naaagaacct ttgatcattt cattgatagc gcctgtcgga cacttggtca
     2281 tgcacgtagc gttgtcgcag ttgtggcaga cagcctgatt gacgacggcc aaatggtcga
     2341 cgatcttgat agcgccttct tcctgatggg agcagttacg catgcaggaa ccgcagccga
     2401 tacacatagc tttgcagttc ttcatggcag cagcgccaaa atctttggag ctgcagctaa
     2461 tagcaacttt ggcactgatc ggcagcaatt cgatgacgtg gttcgggcaa atggaagcgc
     2521 atttgccgca gcccgtgcag agacgtttgt cgataaccgg cagtccgtct tcacccatgt
     2581 gcatagcacc gaaggggcag gaacgggtgc agttaccaaa acctaaacag ccgaatttac
     2641 agaacttcgg accctgctga acgattttag cagcagcaca gtcatgaaca cctttgtatt
     2701 cataagccag ttttgcgatg ccattgccgc cgcggcattt gacgtgggca aatttcggtt
     2761 ccgtagcttc cgcaatttta ccggtcaatt tagcgacgat ttcagcaact tctgccttac
     2821 cgggaacgca gaggttcggc gcaacatcgg gatcaaggac gacagcttct gcatattttg
     2881 cacagccggc aaagccacaa gcgccgcact gaccttgagg cagtgcttct tttacttcgc
     2941 cgataagggg attttcttcc atagcgaatt tcttatccgc acttgcaagg ataaaaccga
     3001 atacaaggcc gatacaaacc atgacgacga cggccatgat tgcaataaat gagtaatcca
     3061 taatcagttt tcaactcctt ttgccttaaa tcatgccgga gaagccaagg aaagccaacg
     3121 acatcatgcc tgcaaggaca aaggcgatcg gtgtcccctt catagcatga gggatatcgg
     3181 caaaatccat tttttcacgg cagcttgcca tcaagataag ggcaatggcg aaaccaatgc
     3241 cggcaccgac agcgaagatg atgttttcga ggaaattata gccactggta acactgatga
     3301 ccgggacgga cagaacgata cagttcgtcg caatcagcat gaggtaaatc ccccacatgt
     3361 tatacagtgt cggagactgt ttcttgatga taatttccaa cagctgtacg aagcttgcaa
     3421 tcaagataac gaaggaaatc gtccgtaaga aggtcaggtt aagcggaacg aggacccagt
     3481 tgtacagact ccaagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnccc atgcccatgc ccatgccatc gaggaaggaa
     3601 acgacgacgc cgtttttcga tgcgaccgat tcagcacgag ccaagatgat agcaaatacg
     3661 acgaccaagt cgaggtatac gccgagggct ttgtacagat caggagcata agcctgtaag
     3721 aacagctgta cacaggttac gatcgtagcg atacacgtaa tataaaccgg aatacgtact
     3781 tttttgttga tcaatttgtg aatgatcgaa acgacgacgt tgttggcggt gataacgaac
     3841 cataccgtaa agcccatggt caaaccgttg acgatgctcg tcgttacagc aaggcccggg
     3901 cacaggctca aggccaagac gaagtacggg ttttcttcaa taatgcctct agtaaagatt
     3961 ttccaaagat tcattattat ttacccccca tctgtgctgc tttttcaaca gcctctttaa
     4021 cgcccaaggt gaaggcacgg gaagaaatcg ttgcaccagt caaagcctgg atcttatctt
     4081 ttgcgcttct gtcctttgtg acttcaagac cggcaatttt cttaccttta aactgatctt
     4141 tgaacggaga ttttgcgcct ttgtcaccaa gacccggcgt ttcattggct tcgaggaccg
     4201 tgtagtccat aaccgtaccg tccatggata cagccgtcaa aagcttaatc ggaccgccgt
     4261 atcctttcgg agccgtcgga atgatgtaag cgactttctg gccgcctttt tcagcaataa
     4321 acgtatcttc tt
//
LOCUS       DAWDXZ010000099         4231 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5187_length_4231_cov_8.239703, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000099 DAWDXZ010000000
VERSION     DAWDXZ010000099.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5187_length_4231_cov_8.239703"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    714..723
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1129..1138
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1128 a         1175 c          857 g         1051 t
ORIGIN      
        1 gtcggtcaat gcctgaagcc ggcgggtcga ttggcagctt ccttcaaata aagtctcaag
       61 gaaatgaact tgggtcgtaa aaataaccgg cgtgtcccag cgttcggtca ataacttccg
      121 ccattcccgc tcttcctcac tcatgtcttc cttgggcaat acgttggaat ggtgctccaa
      181 cacggccgca tcaagctgga atacgtcacg gatttccttg gcattttgat cgataatcga
      241 cgtataagga atgacgacga tgattcgctt tttatggtac ttttgggcct gagctaacgc
      301 aaagcgcata ctagcaaaat tcttgccgct ccccgtcggt accgacaagg tgtagatgcc
      361 cggtttctcc gttccaaagt gttggcaggc atcactgatt ttttgccgtt ccaaggcaat
      421 ggcccgggcc ttaccatcta ccggcaggac aaaggagtga agcttatctt ccaatttccc
      481 ggaaaagaga ttccacagct tatcgtaatc ccaattctgc gtcaaagagg agttcccttc
      541 gaactctgcc gaatccaatc gatcagcatc gactaaccag ctaaaaagca gcctttcgac
      601 catgccccaa taaaaccaat atttttggcg gttttcacga tttttagacg gacagcattt
      661 attaatgcgg tcctggaatt cttcgacttc ggcaacggct ttatggaaga gatnnnnnnn
      721 nnnacctgaa aaggcttttg cccctcaatc cactgactgc ggctcttaga aaaatcatgc
      781 aagaggccgg tcaacttcgc caaggcgccc aagccccaag gtgttgcggc aacttccatc
      841 ttctcagcca cgttcactaa gtgattcctc aaagcctgta ccacaacctt gccgtcctta
      901 tctcttcgaa aatgcgctat aatcgtcata aaagctccta gcaatacgac gtcaggccgg
      961 tctatgcctc aatctaagcc atataccggt tcacgatgcg gcttcctgac aagaaacaat
     1021 cgacattcat atatctctaa aactcattat actcctaaac gatatttact acaaagcatg
     1081 tagattagaa ttaaataaaa attaatgaaa gcatacctcc cttctctann nnnnnnnnta
     1141 aattgacatg tcatgtatcc tttgagccca ttatagtgct tcgttatttc tcaaatcata
     1201 acgtaatatc gagggccgcc tcgggaaaca acgcatacaa aaaaacgccg cgagtcctct
     1261 aaactcacgg cgttcctatc ctttaataga cttcgttatc ttttcgtttc gtcaccgtcg
     1321 gatggctgtc gataatgatc tggcggcgca ggcaagtggc atcgtggtcg ttgatgatgc
     1381 cgcaggcttg gaggtgggag taaatagtga caccgccgac atacttaaaa ccgcgcttct
     1441 tcatatcttt gctgattcgt tccgacaagc cgttgcggac cggaatcgct cccttggcat
     1501 gaccttgata gagaatcgtt tttccgccgg tgaagctcca gaagtagtcg cacaagcttc
     1561 cgaattcgtc gcgcagtttc tgacagcaac gggcattgtt gatgacagtc tgtctcgtcc
     1621 ggggcgtgcg gatcaggccg tcggtcttta ggatgcgttc cacatcggcg tcgtcgtaag
     1681 ctgccacctt atcgtaatca aagttgtcga aacactgccg gaaaatatcc cgcttgcgca
     1741 aaacgatcag ccaactgagg ccgcactgca ggttttccaa ggtcaaatgc tcaaacatct
     1801 gccgatcatc gtgaacggga acgccccatt cgttgtcatg atacgtcgta ttgagcggat
     1861 ccgtattggc ccattcacaa tatcccatct ttttctcccc tttcttaacc cgaaaggcag
     1921 ccctctcttc tcggtcctca tcaaatcaaa ggcttagcgc catgatagtc cttgaaacag
     1981 aggcggcagt cctgacgaac ccgttagcaa acacatgttt ccatatccta attataccat
     2041 cgaaggaagt tcctcgctac cgataaggga tgcgccctcg aaagagaatt tcccattgat
     2101 tctttcccaa acttttgcta taatcagttc aacttcatat gtaatggtac tgactgctgc
     2161 cgagtagtca gagtgcgccg tgttcctcat cgtcaggtct tagcagatga ggacgcggcg
     2221 cctttttagt ttttaggagg aaacaatgaa aacattcttt tacaaggaac cttatgagga
     2281 aaatggaaaa acggtcttag aggtcaatct cttcccggac aaatactgca actttaactg
     2341 catcttctgc ccggtcagcc gaaagttgac ccacatacaa acagaggaaa ccgtccattt
     2401 ggacgacatg gccccggcca tgggcgactt acatcaacgg ctccgtgaca tacgtcccga
     2461 cttagtcttt attaactccc tgggagaagc cctgctctta ccggaactcg aacagatcat
     2521 ccataccatc cacgaagccg gaattcccgt acgcctcctc tccaacggct acctctatgg
     2581 aaatccggaa tacgccagac tggccggtca ctgtgaagaa gtcattggcg aactgaaaat
     2641 ggctcacgaa gcagcctttc aaaaggcaca gcgccccctt ccgggctaca cccttcatca
     2701 gcatataaac aacatggtcc gattccgaaa aaactacagc ggccgcttcc atctggagat
     2761 caccatcgtc aaaggctaca gtgacgatgc ggactctctc gaaattttcc acaaggccgt
     2821 ccaatccata acgcctgacg aacttcacgt cgtaaccctg gagcctccct tcgataaagt
     2881 gttggccatc gctccggaaa ggctcgaaga gattacgcgt tttctgcaag aacctttgaa
     2941 cttctaattt cggtgaagct cagacgccct tagcgaaccg ccctgcaagg tcagccccct
     3001 cccaaaagaa aagccgcaag tccacagacc cgcggctttc atcctcgttt tagaataatt
     3061 gtttcaatcc ctttttccag caacgaatca aataatagat gcttaataat aatacgtccc
     3121 gttgaggcga taggaccagc ctaaccgatt catgccgtat tcaaaaatct tgttgggagc
     3181 catgagcacc gtcgtatgat ttcccgacat ggtattggtc cgataccgcc acatgtccgt
     3241 cttatactga gaaaaacgcc aagtcacgac ctcacccaac ctgccgtttt gaactctttt
     3301 tacagagaca gaaaaccatt tcccggtcgc actcgtccca taggttaagg tatcatccat
     3361 gacatagacg tctacattct ccgatgccat atggttgacc caaacgtcgg cagccccggc
     3421 agaaccggtg ccggcaaaga agcacataca tcccacacaa caaatcaata acgtcttcca
     3481 caaccgtttc acgacctcat ctcctttgtg aatagaatcg cctgtcaatc atacatatag
     3541 cagcgcctta atagtaatgc gtgccgtcac tcgaatagga ccatcccaac tgcttcatcc
     3601 catattcaaa gattttatta ggcaccagga cgcccgtacg acggctgccg gccatggtgc
     3661 tcgtgtaata ttgccacacc gtatcggcct taaagaaccg ccaagtcacc actttgtcca
     3721 acttgccatt ttgaacccgt ttgacagcta cggcaaacca cggtccctgt gaatcggttc
     3781 cggaagctaa gatatcatcc atgacataca aatccacatt ttccgaagcc caatgatcga
     3841 cccaaacgtc ggcagccttc gaaggaccgg cgcagccgaa gaggaccaga tatatcaaca
     3901 aaaagactgc caatgcctta gccaactttc tcacccgctt catccccttt actaaaaata
     3961 acgctctatc ctcatagcaa taggccatcg cagtacacgt catgatagag agtatcgaac
     4021 gatagaacaa ctaattgagc agccatctga ctggtagcaa atacgaaaaa ccccctccat
     4081 tcgattctat atacttccga ttatacaaag ccatcgtgac aaaaccgtct ctattcatag
     4141 aattcatgtt caaaaggccc tgtactgccg acgtttttcc ccctttccgc gtccgttccg
     4201 gtcacaatga attctctatt ttttagtcat c
//
LOCUS       DAWDXZ010000100         4230 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5189_length_4230_cov_9.367904, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000100 DAWDXZ010000000
VERSION     DAWDXZ010000100.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5189_length_4230_cov_9.367904"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2420..2429
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          908 a         1041 c         1142 g         1129 t
ORIGIN      
        1 tgcagcgcgt cggcatgatc gaccgggccg attactatcc ggctcaatta tccggcggcc
       61 agcagcagcg tgtcggtatt gcccgggcca ttgccgtcaa tccggacgtt atcttgtttg
      121 acgaaccgac gtcggccctc gacccggaac tggtcggcga cgtgctgaag gtcatgcgcg
      181 atttggctaa agccgggacg acgatgatcg tcgtcactca tgaaatgaaa tttgcttccg
      241 acgtagctac ccatgtcatc tttatggatg gcggcgtcat cgtcgaagaa ggttcatcgg
      301 aagatatctt caagcatccg aaagaagaac ggacgaaacg attcctgcac cgcattttct
      361 gtaccgcctt tgacggtccg gacgaagagg aataaaagaa gcataataga atataaaaag
      421 agaaggtctt cctctctatc aatcgatagg gaaggagacc ttctttgtat ggtgcagaat
      481 ctgtgttttg aggaatcaac cgatgatgac ggagacgatc tcgtccggtg tatcgacgag
      541 aatggaagct ccgtggcttt tcagaaattc ggtcggccga aagccccagg tgacgctgat
      601 gcagtcgagg ccggcatttt gggctgtctg gatatcgatt tcagaatcgc cgacgtatac
      661 ggcgtcttcc ggtgcgaggc cgaggtcatg gagcactttt tggaccatgt ccggagccgg
      721 cttacggcgg atttgcggct gttcaccaag gacgctgtcg aagaggtccg ggaaatagtc
      781 ggcagccaag tcctgtacgg caggattcgg cttgtttgat acgacggcca ctttgacgtc
      841 cttttcgcgg aggcggcgaa tcatggtgct gatgccgtcg tattcgccgg tctttacggc
      901 acagtgagcc gcataataag gcttatagat gtcctggatg cgggtgattt cctgagggtc
      961 gatttgcggg gtgaccttgt cgccgggctg gccgatggct tcgagttcat catacgaggc
     1021 gaggccctgt tcaatcgcta aggctcgcgt gatggcaacg acgacaccgc tgccgaagaa
     1081 gttgcgaaca tcggctggcg cataagcatg ggcgtggccg gtctgtgcta aggcatagtt
     1141 taaggcatcg gtcaggtcat ctaaggtgtt gaggaccgtt ccatccatat caaaaataac
     1201 ggcattgtat tttttggttg tcatgagaga gcctcctgca tgttcttttt accggaacag
     1261 tttaacgttc aacggcccgg ttgtcaagtc aggctgcttt ggtaaatctt ttctaagttg
     1321 tcctgcatgc ggtcgaggta agacttgccg tcttcactgg tttcaatggt gtggatcgtc
     1381 tctacgcggg cgccgacttc gcgggctaag gtttctgacg tttcgggact taccatgtct
     1441 tcgacgaaga tggtctttac gttatgggcc cggcagtaat cggcaagcct tgccagctgg
     1501 cgggcactcg gctcaccgct ggaaaagacc gattcgacac tttgttgagt caggccgaag
     1561 tctcggcata agtaggcaaa ggcggcgtgg ccggtgacaa aatccttgcg gggcgccttc
     1621 gcgaatttgc cggcatattc gtcctgcagg gcttggagct gccgggtgta gttttcggca
     1681 ttctgctgat agtaggcctt gttggccgga tcggcctcgg acagtgcatc ggcgatgttc
     1741 cgcacttcct gctgggcgtt gatcaggctg agccaaatgt gcgggtcata gtcgccgtga
     1801 tcgtgggctt cttcttcatc ggtgagtttg atcggtgtaa tgccttgaga ggcattgacc
     1861 gtgtgaaggg acgtattttt ggccgcatcg acgacttttg aagcccaagg ttccatgccc
     1921 agtccgttgt agacgaagag ctgcgattga ccgaggcgtt tgatggtctt cgtcgtcggt
     1981 tggaattcat ggggctccgt tccgtcggga atgagggtgt aaacatcgac tttatcgccg
     2041 ccgacggctt gggtaaattc ttttaaggcg tcaaaggtga cggcgacctt cagttttccg
     2101 tcgtctggta catcggccgt ggtgccgcag ccggccagga ctgacaaggt catagccgca
     2161 atcagaagca tcaagaaccg cgccgggaga cgggagtgat gagatgacat agataccgac
     2221 ctcctatggc gttcatcgta tcacgaccgc ctttttttgt caattacttg caaatgcctt
     2281 gcattttcgt ttgcgtagag atataataaa ctaccgataa ttttatggca gcggaggcag
     2341 ttatgatcga tttacaacac gtttacttct cttatgaaca cggcgagtat ttgctcgaag
     2401 atattaatat gaccattcgn nnnnnnnnng accattcgga atggggacta tatttccgtc
     2461 gtcggcgaaa acggcagcgg caagagcacc ctgattaaac tcattttggg actcctcgtg
     2521 ccgagccgcg gcgtcatttc caatacctat cgtcatcggg cttatgtgcc gcagcgcttt
     2581 gaaacgctga acagccagtt tcccatcacc gtctgggaag tgctcaactg ttaccggcaa
     2641 gtccttcatt taaaagataa ggacctcata cggacttgtt tagagcagat gggggtctat
     2701 gatttaaaag accagctcat cggcagttta tccggcggtc aatgtcagaa agtcctcatt
     2761 gccagagctc tcatggggca tcccgatctg ctcattttcg atgaaccgtc gacgggcatc
     2821 gacgtccgca gtcaggaaga attgtacccc tttattaagg atttaaatga gaatcaaggg
     2881 attacagtcc ttacggtaga gcataatttg aaatttgccg tccgcaattc gacgaaactg
     2941 ttccaagtcg tcggaggcca tggccacttc tgctctccga aggccaacct cgatgaatat
     3001 atgtcggtta cgatgggagg ggaagactga tgtttgaata tgcttttatg caaaatgcct
     3061 ttatcgtagc catcctcatt tccattgtct gccctctcat cggcatcttc ctcgtcctgc
     3121 gccgctattc catgattggt gacgccttat cccatgcgtc tttagccggc gttgctgtcg
     3181 ggttactcct cgattggaac cctgtcctcg gcgcctttgg cctgaccagc tttttcggca
     3241 tcctcattga agttctgcgc agtcgtttca gacgttatgc cgagttgatt ttggttatta
     3301 tcttgtccct ctccgtcggc cttgccatta ccatcatcag tgccggactg gttcatacca
     3361 atgtagagtc tttcttattc ggcagtatcc tcacggtctc gacggacgat gtgtacgccg
     3421 ttatcgccct cagcgtcgtt tccatggccc tcattgccaa gctctatccc caactggtca
     3481 tgctgacctt cgatgaagac ggggcccaga ttgccggcgt tcacagtaaa gtcgtcaatt
     3541 atattttttc tgtcctggta gcggcgacga tttccgtatc cattcgcatc gtcggtatct
     3601 tggtcatcag ctctctcata gcccttcccg tagcggcggc cctgcagctt cgaaaaggct
     3661 ttcgtcagac cctgatgtat tccatctttt tcagtttcgt cgatattttg tcgggtctgt
     3721 tcttgtccta ttatatcgat gctgcccccg gcggagtcac ggccctgacg gctgtcgccg
     3781 tcttggtgct ggtcatcatc tataaagaag ggattttatc atggagagaa aagaggcgtc
     3841 agtcatgagt gatacgtata aagactgcct tcgtcaacat ggaatgaaga gtaccaaggc
     3901 ccgcaatctc gtactggatg tcttgagctg taaacagaca ctcgtcacgg tcgaagccgt
     3961 ttatgcggaa cttctcgaag gcgggaataa ggtcagtttt tcaacggttt atcgaatctt
     4021 ggaaatgttt acccagcggg atttgacgga aaaaattttt cttcccgacg aagggaaata
     4081 cgggttttct ctgcggcggc cgggccatac acaccgttta atctgtttac agtgccatca
     4141 aatcgtagac ttaccgcact gtccgctcca tgattatgaa cgagaagttg aaaaaaagac
     4201 tgatttcgat atcgtcagcc ataatctcga
//
LOCUS       DAWDXZ010000101         4225 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5200_length_4225_cov_9.100959, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000101 DAWDXZ010000000
VERSION     DAWDXZ010000101.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5200_length_4225_cov_9.100959"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1028 a          996 c         1182 g         1019 t
ORIGIN      
        1 cgccatatgg aagtgggcat catgattgaa acgccggcgg cagcggtcat gagtgacgag
       61 ctggcaaagg aagtcgattt cttcagcatc ggctccaatg acctgactca gtacacactg
      121 gctatcgacc gtcagcagac gacactcgat tccttctttg atgcccatca tccggcggta
      181 ctgcgtctca ttgaacagac cattcaaaac ggccataagg ctggcatctg ggttggtatc
      241 tgcggtgaat taggggcaga tcttaccttg accgaactgt ttcttcgcat gggggtcgat
      301 gagctttctg taagtccatc ggctgtgctg ggcctgcgtg aaaaaatacg ttcccttgat
      361 ttgacaaaat aaattgtcca ttgtacagta agaaagagtc tgtacaacgt gagattactc
      421 attgtataca ggctcttcgt aatttcgatg ccccagggta tcgtggctga ggaggatata
      481 tggatagacg aaagctattt gtgtttgacc tggatggaac tatgttcgat acggaaatta
      541 tttcctatca gtgttggcgg gacgtatcgg cccgttacgg attccaaatg ggacgtgatg
      601 tatttgatct catgttgggc cgtgacaacc tgcgcgtccg ctctacctgt gaaaatgcct
      661 ttgggccgac ctatccctat gaccgggtgt gcaaggagaa gatagaactg cagctggagt
      721 actaccacac ccatgatatt cctatgaaac cgggattact ccatatcttg gaatacgcga
      781 aagaacaggg catcctctgt gctgttgcct cgtcgtcacc gctgaaactc attgagtatc
      841 tgctgaaaaa gacggctatt gattccttct tcaccgtcgt ccaaagcgga gaagaagcag
      901 cccatggaaa accggaaccc gatatctttc tcatggcttg tgacaaggcc ggcattgcac
      961 cggaagatgc cctggttctg gaagattcag aaagcggcat cctggcagct catgccgcag
     1021 gaatcccgtc cgtatggatc ccggaccttg tgacgattcc cgaagaggtg cagcagttgg
     1081 cttggcaatg ctgccaacgt ttggatgaag taccggctcg gctagaacaa aactatggta
     1141 agacgcatat gaacggctaa aaatgatgtg cccaaaaaag ttagatataa ccactgcagc
     1201 aaacaggctg cggtggtttt tctatgcaga ggcatggcta agtacgtagg ttgccggact
     1261 gaatctgccg tgtgtttcct cgggtgctta cgtatcggtt gagctgcatg aagcttagcc
     1321 ttaaaagggc atctactata aaaatccccc taaactttgg attttttgtc caatgtcagg
     1381 gggataatct gttgcttaaa gggacagatt caatggtttt cttaactgtg cagatttttc
     1441 caagccttcg gtacggggat gaggaaggcg aagatggagc ctatgacggc ggcaatccag
     1501 aggatttgca gggctgtcgt caggccccac tggtcggcac cccagccgac aaggggtgag
     1561 accagtccgc cgatggtcgt cgtaaggccc agggtgacgc cggaagcaaa gccgacgttt
     1621 ttcccaaggt aggtctgtcc taagacgacg atagggctgt aggcggcaaa gagggagaag
     1681 gcgacgggca ggagcagtaa tgtcgccgtg acgatattgg tgctgttgac caggaagaac
     1741 atggacggaa tcatgagcag catggccccg cgcagaatgc ggatgaagcc gaagcgatcg
     1801 gccaggatgc cgccgaggaa ggtgatgacg acgcccatgg agaagaggat tgataaggcg
     1861 agactccctg tcgtcggggc agcgtggagg acgttgatcc aaaagagggg aatgaaggcg
     1921 ttgcagagtg taaagccgat ggaacggacg aagatgatga ccgacagacg gctgaaggca
     1981 ccccattcgt tttccttgac ttcgccggga tgggcttttt gttctgcttt tttggattcc
     2041 tgtacgaggg acaggatgtg gggcatacgg ttgtagatga tgagggccag gatggtgttg
     2101 acgatgccga agatgacaag accgcggata tcaaaggcat aggcacaggc accggcgacg
     2161 atcggtccta cggcaaagcc ggcattgccg ccgacggaaa aggatcccaa ggccttgcct
     2221 ttttggtcgc cggcgatgct attgaccatg cgggctgctt cgggatgata gatggaggag
     2281 ccgaagccgc tgaacatgga gaagaggaag atgagccagt agttctgcgt aaaaccgaga
     2341 agggtcaggc aaatcccgca gaggatggga cctaagggga cgaaccaggg ttttgaaatc
     2401 ttatcggaat aatatccaaa gatcggctgc gacaccgagg ataagagcat attggcaaag
     2461 atgagggtcg cgccgtcttg gtagctgagg ttacaggtcg tgatgaagta gggcaggagg
     2521 gccggaatgg cgccttgtgc ccagtcgacg gaaaaatggc ccatcgtaaa gagatagaca
     2581 taggggcttt gttttttcat gaaggtcttc ctttcgtttt tttatcgtta ttattataac
     2641 acaagattgt tttctttttt ttggcgatat ataatagaat atatttattg ctaaaggagt
     2701 tgtgtgagta tggcatcaaa agaaatgtac gccctcggtt ctcaggcttc ggtcatccgc
     2761 gacctctttg cctatggcca ggaacaggca gccgtcgtcg gcaaggacaa tgtctttgac
     2821 ttcagtatcg gaaatcccac cgttccggct ccgtcctgtg tccgtgaagc gattgaagat
     2881 attttgcgcg aacgcgaatc ggcagctatt cacgggtata cggcggcagc cggcgacggt
     2941 cccgtccgtc agggcttggc tgattttatg aataagacat atggcgccga tgtacgggcc
     3001 gacaatttct atatgacctg cggggctgcg gcttctttag tcatcagtct caaagccatc
     3061 gtcgaagacc cttctgatga agtcatcttg gtggctccct ttttcccgga atacacggtt
     3121 ttcgtgcaga atgccggggc taaatcggtc attttgccgc ctgatacgga gcattttcaa
     3181 atctctctcg atgccatgga aaaggcgatt acgccgcaca cgcgggcggt catcatcaat
     3241 tcgcccaaca acccgtccgg cgtcgtctat tcggccgaaa cgtatgaaaa gttggcggct
     3301 ctcctcgaac ggaaatcgaa ggaaatcggc catcccattt atctcatttc cgatgaaccg
     3361 taccgggaaa tcatttatga cggccttccc attttgtacg taccgaagtt ttaccgcgac
     3421 acgatcatct gctattccta cagtaagtcc ctcagccttc ccggcgaacg tatcggctac
     3481 atcctcgttc ccgactgcgt caccggcgct aaagaggtct atacggcggt ctgcggtgcc
     3541 ggccgcttca tgggctttgt ctgcgcaccc catctgttcc aggacgtcat cttgaaatgt
     3601 atgggccaaa cgagcgacgt cgacgtctat gaccaaaacc ggaagttatt gtataatggt
     3661 ttaaaagaaa tgggttatga ctgcgtctat cccagcggtg ccttttatct cttcgtcaaa
     3721 tcgccggaac cggatgccaa ggccttttcc gataaggcca agaaactgaa ccttctcatc
     3781 gtgccggcag acagcttcgg ctgcccgggc tacgtccgca tttcttactg cgtcgatccc
     3841 gatatgattc ggcgttcctt tgcggccttt gaaaagctca tggcccagta tcgctgaaga
     3901 cggtcggtta tctaaaggga gagggccgtt tgactacata ggataaataa aatgtaaagg
     3961 atttggaata attttcttga cacgttctgt atctgtggta tactaaacaa gtcgttagga
     4021 aatgggccta tagctcagct ggttagagcg cacgcctgat aagcgtgagg tcggtggttc
     4081 gagtccacct aggcccacca tttctttatg actgacaatc ggggataagg aaacgtcatc
     4141 ccatacatgg gcctatagct cagctggtta gagcgcacgc ctgataagcg tgaggtcggt
     4201 ggttcgagtc cacctaggcc cacca
//
LOCUS       DAWDXZ010000102         4169 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5256_length_4169_cov_7.618619, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000102 DAWDXZ010000000
VERSION     DAWDXZ010000102.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5256_length_4169_cov_7.618619"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3635..3644
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          935 a         1061 c          911 g         1252 t
ORIGIN      
        1 atatcaccgc cggcaaaggc attgagcagg gtcgacttcc ccgcttttac ctcacctaag
       61 atggctgcat gcaggggctg atctaatttc gcatacagta gttccaactc tttgcgaatt
      121 tccggaagct tggtaaaatt cttatcatga tacaagggcg attgcgctaa tcgctctatg
      181 atttgcgcga tgtgctgctt aacttccatg gtgattcctc ctgtctgaaa acagaatatc
      241 ctttaacaac aagggtctta tggtgagtcg gctcctttcg cccagttgta acagatcttt
      301 tataatagcc tctttctgat ccgatgggcc atacagttcg gtatacgtat cttcccgcaa
      361 ggtctcgatg gattcatcgg cccggtcgag ataatcctca tattctttag ataatttttc
      421 tacagccgtg ccgagttgac ttgaaagacg cttctgtacc tttacagaca tactgtcccc
      481 aaacgtacgg gagagccatt tggccacgct gcgtccgata tccgtaacgg caaaaccggc
      541 aaaaatcaat ccgacaggcc ccaacaaggc ggcggcacca atggctaagg ctccacccaa
      601 acaaaactgc gccgttcctt cgtcgcttaa gtcccccgat atagccgcat cgttcacagc
      661 atttcccgta gaagccgagg cattcatctg ccgattcttc aataagggaa attcccggaa
      721 aggcgccagt gacatatgac gagctgcaat cgttgaaagc tggctgcaat ggtgctgtac
      781 taacaactcc aacgttttct caatctgacc ggggcgaatg attttatcca taatgtagga
      841 atttcggagt ccgctttcca tatcccaaag acggtcttca atcttgctcg tttcctgtga
      901 aacacggtcg gcttcctgct tctggaaatt cttcaattct tgctgtaacc gctgcaggac
      961 gtcggcctta tctttttccc aattttgaat tttatttacc cgttcgccgt ccattttctt
     1021 caagacgtca atcgcttcca tagacgctga gcgtacgtcg ccgtacaatt cctgacaatt
     1081 ccgggtcttg ctttgaacct gtaattgcaa agaattcgcc aggaaggtcc gattcaacaa
     1141 gtcaaacaga gcctttaaat tactttgctc caagagctgt tgtccgcgtt cgtagtcttc
     1201 ctgacttaaa tcgggctgct tcaaaagtat ttctgcttct cgggcctctt tggctgaaag
     1261 gggaataata tcgggaatca tgctgccata taatttttta gcctccgtta cgacttcgtc
     1321 gacgtctttg ccggcctggc gtatgagatc gatacgattg acgacggcaa cgaggttatc
     1381 aggatgtttt tcatacttgg cgtaaagctc tttcagctca ttatagtcac cggaactgtt
     1441 tatcttatct cctggtaaaa tccaaataac gccgtcagcc ttagaaaaat attcgttcgc
     1501 actggcatag accatgtcgt ctaactcttg acgcagtccc ggtgtatcga cgaggcggta
     1561 atgttctaat atcgaactgt cttgcactgg ccacgccgct tcgatgacag ccgatttata
     1621 cagttcgtac tttttcaatt cctgctgctt ttcttccagt tctttcggcg gggccccact
     1681 ggcggcaaag tctttcaatt tcttggaaat agccttacct gattctttgc acttcttctc
     1741 ctctgccgct aaaaattcct gcgcctcttc atacttcaaa ctcttcttcg ttccatcccg
     1801 aaaagtgagg gtacaatccg tcccttcacg ctgctggaaa atatcgattt tccacgtttt
     1861 cggcacaatg ccttcagcgg cctgctgctg ccccagcaag gcgttaatca gcgttgattt
     1921 cccattttta ccatttccca taatgaacag ggtaaaagga taggtcaagt cctctgctaa
     1981 ctgttcaaca gccgtacaat catcttgtat ctgcccgata taatcatgga gcttagccgt
     2041 cttcttatga ttgcaaaacc tgtcaatggc attcatatgt cgtttgatag gttccaaggg
     2101 agccgtcatt tgttgaagta agtgctcctg tatctgccgg ttatattccg cttcgatatg
     2161 ggcataaagg gactggacgg cggccactgt atctttcgta atcataagcc atctcctata
     2221 aaaagattcc ccggaccata taaagcacga ttcctatcat gatcatcaag ttgaagaaca
     2281 tcatatagga caatttttta atcatcgttt gtaagaattc agtctggctg atcaggttcg
     2341 actgcagcgt ctctttggtt tcctgaattt gtttttgtgt tgcctgaaaa ttctggcttc
     2401 tcgtaagggc gcgtttgttt tctgcgttct tttgatcgag ttgaatccgt tcgacatcac
     2461 ctatccgctg tttcaatgcc gtgccaacct gattttggtt ccgttccagc tgctcaattt
     2521 ggctcagcat cttccgattg cgctcgtcaa ttttatgtaa gagctgattt tgtttctcta
     2581 accaattatc cagccaggag tcgacgtgct gattaaaatt ttgcagagac tgttggctat
     2641 gagtcgatac gctttgatac atgtccttgg cttggcgaat atcctttacc agctcgcccg
     2701 attcagcctt cagcgttttc aaggtcccat tcagttcatt gacggacgct aaagccgaat
     2761 gacgtacgac atcgtcggtt acctctttcg tcacgtctgc aatcgtttta ataaactccg
     2821 cttccaatcc gtttgccata aaaatcaccc tttatcctcg tatttatacg cccttttccc
     2881 cgctgtattc agggatttca gcatatatag ccctttactg ttgttaaaat tttgttaaaa
     2941 aacaaatatt ttatacagta tattttctac attactgtaa tataatcctg ctggcaacta
     3001 attttcctct gcaatttttt tgcaacgaaa acactccctt tgaaggacgc ctgccggaaa
     3061 tcccggtatt tgtctggttc aaagggagta tctttatgtt acgattttac ggtcttacca
     3121 aaggtttgga gcctatttgc gtttcgcaac gcgtacccgt tctaccgtcg gattgccgtc
     3181 tttgtcggta tggacggtga accgttctaa ttcaggagca aattcggtga gaatcttgtc
     3241 cgacagggtt gcgccgttta acatggcccg gtatagcatg gcggcgaatt cgtagcgcgt
     3301 catggggcgg ttgccgtcga aggtgccgtc gggatatccc tgaatcatgc cgtttccggc
     3361 cagggttgcc acgtattcat acgcccagtg attctggggg acgtccggga agagctggag
     3421 cttcgacgta tcgatcttgc gtccggcgtt ggcgtcgagc atggccgact tcatagcttc
     3481 cagttcacgg cgcaggtcct tgatttcctt agccatggct acacgcgatg tcgatacacc
     3541 attgctctga ccgaacttga aggagacgcc ggcattgacc atgttgttgc cgttgccgac
     3601 ggttccgccg acactgaaca gagtatcttc gttannnnnn nnnnattgcc gtaaccggca
     3661 gcgaaatccc acttgtcgtc cggatcaaag tcgagcggat gaagggctgc caaagcggcg
     3721 gcaccggcac cgacttcatc gatgcggttg tccagttgat tgaccttgtt gcccaaattg
     3781 ttaatggcca ggctgttctg ggtaacttgc tgattgacgc cttggagctg ttcttccgta
     3841 gcggcacggc ctacggttgc aaagtctttg gaatcgaggg tcgtattggt caagcccgtc
     3901 acttcgccgt cagcaccatc taactcaata ataccaattt cacccatacc ggtcgtaccg
     3961 ttaagggata ctttcttgcc ttcttcggtg ccgattttca gtgaaccttc gccgccgtca
     4021 acgacgacct tcgaattccc ttcaccgagg gtaatctttt tgttgaggcc gaactttaat
     4081 tttgcatttt ctttatgtac gaagacgttc tggttgcctt cggcatcgac atggccggag
     4141 aagtcaaccg tcgtattctt gcttacatt
//
LOCUS       DAWDXZ010000103         4100 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5346_length_4100_cov_6.975031, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000103 DAWDXZ010000000
VERSION     DAWDXZ010000103.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5346_length_4100_cov_6.975031"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1198..1207
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1463..1472
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          889 a         1046 c         1076 g         1069 t
ORIGIN      
        1 ctgccgtcat cattcacaac ggcagtcccc gtcttttctg agcccgggtc aatagcaaaa
       61 atcatggtct tcctccctta cttaccggct tcgacgcgga tgtcaatctg cagcggtcct
      121 gccgtatagg tgtcggtttc cgccacagct ttgaggacga cagcggtccc ggcatggctc
      181 cgaatgcgat tgatagtgtc gaacatttcc gaggccgtca aagcgccgac gttgcccgtc
      241 aaaggatccg gcaagagacc cttagtctgg gcctgttggt ttacattgtg caggaagcgc
      301 atgacctgca gttctgtatt acccgacaga tcatcaccgg tcaacgtttc ttcaaagacg
      361 acgtcgccgc gatgataaat caattggtta tcatacattt cgacatgaac caaggccggc
      421 tcgccgagga tgatgttgcc ggcagcgatg atccgcacca atttttgcga tttaccggct
      481 ttttgcagcg catcgacggt ggcattgaat tcttccggag aaatatacag caaaatcgta
      541 ttttcatcag aaacgccgag atggcggcca atcatcgtgt tggtctgact catagcccga
      601 ctcagttctt cccgcgtctg ttcaatggaa agaccgccgc gaaggacgtt gctcgaaagg
      661 acttcgccga cgcggaatac gaccgtacct tcacggatat tggtcatcgt cgcgttgagc
      721 agcttaatgc gcttgtcaag gccgtcgatg gtatccatca ttttattctt tgtttcttcc
      781 agcatagcca cgtcctggcg cgctttttca ctgtcggcca gggccgagtc acgctgcttt
      841 ttcgcctcat ccatagccac ctgaatgacc gtcagctgac gagccttgtc gtcgcgagcc
      901 agttgagcct gctgaaggtc catttgggtg gcacggattt cttcgttttt cccatcgagc
      961 tgttcctgtt gttgttttaa cagttcttca ttagacgcaa tcagttggtt cttatcatca
     1021 agttccttgc caagctctac ggcctgctgc ataagctggc tgcgctgggc ttcgagctgg
     1081 gctttctgca tctgaagctg atgcatgccg aacaaagcga tgcggacgtt ttcggaaagg
     1141 atggacatga cgcctaaggt aatggcggcg atactgatgc cggtcaggat ggtgactnnn
     1201 nnnnnnntca ttatattata gactataaag gagacttcct gcaagcgtga aggaacagaa
     1261 aggctcccat tatcccaaaa cggtcgaagt cctatcgtat ttcagtcaca atcgagactc
     1321 tgattttcgg gctctatttc ataattgaat ctcaaaaaaa ggaactgcct atcggacagt
     1381 tcctttgctt acttcgattt cttatagact aagatgacac cggcgatacc gcagatgata
     1441 tcgggcaaaa aggcggcgat atnnnnnnnn nncgttcccc agggcattgc cgagggtcat
     1501 gatcgagtaa tagataaaga tgacgacgac actgatgcca aagccgatgg aagagcttcc
     1561 gcgctgcttc tgcattccca atggggcacc gacgacagca cagacgaggc tggccagggg
     1621 cagggcaaag cggttgtaca tttcgacttt catcttattg gtatcgacag agttttggtc
     1681 gaggagtttt atctgttcgc gcagttcacg aatggtcaac tcatccggtt ttttctgaga
     1741 ggccgaaatt ttatccggct tctgcgtcac cggaagggcc tggttttgga acttcatgga
     1801 ccgcgtcgtc cgctcatccg tcgagacgtc aaaaatcgtg ccttcgtgca tgatccattt
     1861 ctgtccgttc cagtcggccc tgtcagcctg ttcgacgcgc ttgagctggt cgttttcaaa
     1921 ttcctggacg gtaatatcct tcaattgcct tgattccgca tcgtactgcc gggcatacat
     1981 caggctggat atatcggagc cgtctgtatt tttgatgata atatgatcct gtgtcttcgg
     2041 cgcgacgttg tgctttattt cgtcgttaat aatccggtta taggcgttat tggccttggg
     2101 cacgacgtat tcattaaagg ccgtagcact gagtgaaata aacaaggcgg cgacgaatac
     2161 cggcatggcc aggcggatga agttttgacc gccggcccgc atgacgatca tttcactgga
     2221 acttgaaagg cggccaaagg ccatgagcga acctaagagg acggacatgg gaaaggtgat
     2281 gacgatgata gagggtatgg ccagcacgaa gacgcgaaaa gccgcccaag gagaagcgcc
     2341 gtactgattg atgaggttgg caatccggta cagcgtccct gtgccgatga aaacggccgt
     2401 aaaggcgcag acgccaaaca gaaaggggcc gataaattct tttaatatat acttgtctaa
     2461 tatacgcatt ccatactccc tgttacatcc tgaattcttc gcctaagtag aatttcttgg
     2521 cgaccgggtc attggcgatg aagtccggac tgccttcgag gaggattttt ccgtcactca
     2581 aaatataggc tttatcgacg atgctgagcg tttcccggac attatggtcg gtaatgagga
     2641 cgcccatgcc gcgccgtttc aaatggctga taatgccttg gatatcgttg accgctaagg
     2701 ggtcgatgcc ggcaaaaggt tcgtcgagga gaatgaaagc cggatcggtc accaggcagc
     2761 gggcgatttc gacgcggcgc cgttcgccgc cggagagctg gatgcccagt cggtcccgca
     2821 gctgggtaat ggaaaactct tccatcaagg cttccgcctt ttcatgctgc tgttttttgc
     2881 tgagcttcgt cgtttccagc agggccagga ggttgtcctc tacggtcagc ttacggaaga
     2941 tagatgcttc ctgaggcaga tagccgatgc cgtgggccgc ccgttggtgg atgggaagct
     3001 gagtaatatc gtttccgtca atcgtgattt cgccggatgt cggccgctcg atgccgacaa
     3061 tcatgtagaa ggtcgtcgtc ttccccgccc cgttaggccc gaggagacct acgatttctc
     3121 cttggtcgac acgaatgctg acaccgtcga cggcatttct ttctttatac gattttacga
     3181 gatctttggt ctgaatgaac atggataccc cttactcact gcccggcacg atgacgatgg
     3241 tcgagcggct gcgagtctga acagaattat cggcaagttg aatatcgaca ttatccccat
     3301 ttaaggaatt gccgttctgg gtcgcattga cgttgccccg gagatggacg acgccgtcat
     3361 tttggcccgg agtctgggtg tagatagcct gattggcata ggccaccagg ttgtccgccg
     3421 gcgattcgat atggacattg cccgtaccga cggcctttat ctgtttaaac caggcatcga
     3481 tgtggtcggc ccacatctgc gacccctgag cttcgagata gccgttgccg gtgacgatgc
     3541 tgtgatcctt gtcgaggtag tattctacct tatcgccgcg aactttttta tcttcataga
     3601 caccgtggac attccccgtg gctacgatat agttgcggtt cgtcgaatgc attttatctg
     3661 aattgagctg catattcggc tgctgtacgg caacattgcc ttccatgtcg gcttcggacg
     3721 tcttcaagtt gtagacgcct ttattgccgg tcatggtcgc ctggtcgcgg acgatgacga
     3781 cgttgcccgt agccgttgcc acctgcgtct taccgttgta ttcgagcttg tctgccgtaa
     3841 ccgatacggc aggattgccg ttgttatcgg cggccatgac cccggcagac ggaaggatca
     3901 aggacccgat aagggcagcg gctgccaatt ttcttcccaa gaattctatc ataattattc
     3961 tccccttgtc acttgcgcat ggccggtcag caagaccttg gtcagactgc tgtcggtttc
     4021 aaaagaatct gctttcagcg tcgtattatc atggcgctcc atgacgactt tgccgccttt
     4081 aatcatacgc gtatccatat
//
LOCUS       DAWDXZ010000104         4085 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5364_length_4085_cov_6.993797, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000104 DAWDXZ010000000
VERSION     DAWDXZ010000104.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5364_length_4085_cov_6.993797"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    639..648
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1770..1779
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2371..2380
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3560..3569
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          890 a         1030 c         1043 g         1082 t
ORIGIN      
        1 gtaacgtcgt cagtgactaa ctggagggca tcgcggtcac cggtaacgac gtcgacggca
       61 tagccttcag cggccatgtt tttcgcaagg gtaccgatga tgtcatcccc ttcatagcct
      121 tcctgttcga ggacggagat acccaggcag tcgaggactt cgcggatcaa ggcgaactga
      181 gggcgcaggt cgtcgggcgc ttccttacgg gtcgccttat agccgtcata caagtcgttg
      241 cggaaggtgt actttccctt gtcgaaggcg acggcgacgt actgcggatc gagttcgctg
      301 taaagcttca ataacatatt caaaaaacca tagacggcat tggtcggccg tccctgagga
      361 tcactcagat ggggaatggc aaaaaaagcc cgatagagca ggctgctgcc gtcgatgatg
      421 acgatattct ctttcatact ctaattggcc ctttctttaa tatatagtct agcggtgcag
      481 gatggagctc aggatgctgt acaggcagta gatgacaaag atgccgagca gataaggcat
      541 ggccatgacg atgagtccca aggcaccgat gatgacggcg acgacgcaga agacgatgac
      601 gccgatggct aattttacgc gccatgacag accgctggnn nnnnnnnntc ccgcagccgt
      661 tcttcataaa gggactgctg gtcgtggacc gtaccgtctt catcaatggt cggtccgctg
      721 aagttgtctc gttcgtgatc cgagagaatt ttgggatctt ctgaattcat aacgtaatac
      781 tcctatcttt agtaatttct gtttttcata cggcgttcaa tatcgcgttt ggcttctttt
      841 tcagccaagg cctggcgttt atcgtacagt ttcttccctg tcgctaaggc cacggttacc
      901 ttgaccttcc ccttcttaaa atagagattg aggggaatga gggtatagcc tttttcctgg
      961 atcttgccca acaatttgat gatttcatac ttgtggagca agagcttccg cgtccgcagc
     1021 gggtcatggt taaagcgatt tccctgttcg tacgggctga tatgcatccc taagagcagg
     1081 acttcgccgt tttcaatgcg gcagaagctg tctttcaaat tgaccttgcc ctggcgcagg
     1141 gatttgactt ctgtaccggt cagggcgatg ccggcttcat acgtttcatg aataaaataa
     1201 tcgtgacggg ccttgcggtt atccgaaata tatttcacac ctttagtcgg tgatgttgac
     1261 actagtgatt cctcctactt cttactcttt ttattgcggc gcactttggt attcttgcct
     1321 ttgcggtcgt ttttacggct gttcttctta tccttgcgga ttttcttatc cttcttttta
     1381 tctttcttcg agccgcctgc gccggcgaac caattgcgct tggtcttgcc ttccttgttg
     1441 tcgctgctct ttcgtttatg gcgccgttca ctgctggcgg ccatcaactt ctggacgtct
     1501 tccagcgatt catagcggcc gggaacgaag tcgatttctc ctttttcgac gttgacctga
     1561 gccaaggtga cttccatggc gtcgccgaga cgataaatct taccgccgaa ctgacctcga
     1621 agcgtataag acgtttcgtc gaattcgtag gcatcatcgg tcaacaggga aatgtggatc
     1681 aaaccttcga taccgttttc gaggcctaca aagagaccaa aggacgtgat ccctgtgaca
     1741 tgggcttcga aaggctggcc gatgaagggn nnnnnnnnng ttatcgctgt cgcggagata
     1801 gcaagagacg gtttcgttgg caatgagcat gctttcttcg acgatctgtt cggccagggt
     1861 ccgttcccgc ttttctaagc gaaggggcac gccttccggg tcgaggatga ccttgtattc
     1921 cgggaaatca aagtcgatgg ccccgcggcg aaggcgcata ttctttaaga ttgccgacag
     1981 ttcctgcaaa tcccggagca tgggcatgaa gggctgcaaa tcctcaggga tgatgccgtc
     2041 gaccagggct tttcggactt cgacgtagtt acagcggcgc ttgacgcgga tgacagccgg
     2101 tccgatgtca gatttgacga ccttgccctt atcatcgatt tccatgacac aggtcatgga
     2161 atatcggtct tccccggcgt tgaggctgca aataccgttg gacaggactt ccggcagcat
     2221 ggggatgacc cggtcgacga ggtagacact ggtgccccga ttataggctt cttcatcgat
     2281 ggccgagtga ggacggacgt agtagctgac gtcggcaatg tagacgccca ggcggaaatg
     2341 accgttgggc agttttacga cgtcgatggc nnnnnnnnnn ctttggcatc ttcactgtca
     2401 atcgtaatga gctgacggtc gcggtagtcg cggcggccgt cttcgaggac gacggtctgg
     2461 tcgatggcat cggcttcggc cgttacctct tccggaaagg caaacggcag gccgtggcgg
     2521 gccatgataa ccttgatgtc gaggtcgcgg tcgccgttat agccgagaac ttccgtcact
     2581 tccccttcgg ccttgcgatt ccgtgtcggc catttggtaa tcgtcaccaa gacgcgggca
     2641 ctgctgcggg cattgcccgt tttatcgaga ggaatgtaga tatcttcacg aatccgttca
     2701 tcatcaggct ttacaaaagc gctgtgtttt tccaattgga aggtaccgac taaggtctgg
     2761 ttagctcgtt cgacgatgga aatgatttcc ccttcctgct tgtggcggcc gttcccggca
     2821 tggatgatac ggaccgtgac cttgtcgtta ttcatggccg tgttgcggtt ctgctcggaa
     2881 atatagacgt cctccgtcag atcttcgctg atgacaaaac caaaggtcgg cgtgtatccc
     2941 ttatagatac cggtaatttc ctgcggcgtt ttggcagcaa tatatcgatc gcctgtaatt
     3001 ctcatgagct tctcactcct caccaattca tcaaatgttt caaatacgtc agctaatctt
     3061 gcaccggcaa tgccgagctg ttcggcacag tcttcgacgg tcgccccttc gggggccatg
     3121 gcagtacata tatttattat atcatgagcc agctttaaat ccatagtgct cctcccgaca
     3181 tgtccgctta taaaaaagag ccgccgcata tgcgacagcc ctcattagtt tcattaaaag
     3241 gtatgcatca atctgcccaa cacgaaggta cagacggcaa acaataccga taagatgata
     3301 gtcattttgg aaagcaaggc atccatgccg cgggcacgac tgccaaacag gtcgtcggca
     3361 ccgccgccaa agcctgcacc catcccctgg gtcttgctct tctgcgctac gacaacaccg
     3421 atgatgagca gtgccaaaat gacaacgatg atttctaatg cagtgatcac gttaatcctc
     3481 ctcacacaat gacgctaaca gtattattgt acacttattt agcctttcta tcaatagttg
     3541 atttcggctc tttctcaatn nnnnnnnnna atagtgctcc aatttgcgtt tgacccgttg
     3601 gagggcatta tcgatggatt ttacgtgacg atggagttcc cgtgacattt cattatacga
     3661 tttcccgtct aaataggccg tcaagacgtt ccattccaga tcactgagga tttcgttcat
     3721 cttcatctca atgtcgttga attcttcccg tccgatgatg agctcttccg ggtcgctgac
     3781 gcgaatgccc gacaagatat cctgaagggt ccggtcggaa tcttcatcat agatgggctt
     3841 attgagggat acgtaagaat tgagaggtat gtgtttctgc cgcgtcgccg tcttaatggc
     3901 cgtaatgatc tgccgggtaa tgcacagttc ggcaaaggcc cgaaaggacg acaatttatc
     3961 accgcgaaaa tcccgcgtcg ccttataaag gccgatcatt ccttcctgga tgatgtcatc
     4021 ccggtcggca ccgacaagaa agtaggcgcg agccttcgat tttacaaagt tacggtactt
     4081 gtgga
//
LOCUS       DAWDXZ010000105         4062 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5392_length_4062_cov_7.483154, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000105 DAWDXZ010000000
VERSION     DAWDXZ010000105.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5392_length_4062_cov_7.483154"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1062 a         1051 c         1041 g          908 t
ORIGIN      
        1 ggccgtctga ggctctcgcc ctgacagctt gttaagtata ctacgccaag aattccttgt
       61 caagcaaaaa gttgacgatt cctaaagttt cccttcaaag gcgtcccctt ttatagtata
      121 attatgtgga agttgtattt tattatttcc gtttacaaaa aaggaacagg agttgttacg
      181 tatgaacaaa aagtatttag ttatggcggc aactgtagct gcagctgcta ccttactcgc
      241 cggatgtggt tcttcagcag acaaacaggg cggtgctgcc gatgagcaga aaaccctcgt
      301 cgtcgggatg gaaccgactt tcccgccttt tgaattcacg gacaacggca agtatgtagg
      361 ctttgatatt gatttagcca atgccatcgg tgaaaagacg ggcatgcagg ttgaagtcaa
      421 aagcctcggc ttcgacgccc tcattccggc actgcgcagc ggccagatcg acatgattgc
      481 ttccggcatg gacgctactc cggaacgaca gaaacaagtt tcctttacag aaccgtactt
      541 ccaggacggc tattccgtcg tcgtccgcaa ggataacgac aacattcacg gctttgatga
      601 actcaaaggc cgtaaggtag gaactcaggt cggcaccaag ggcgtagacc tcggaacgga
      661 agccggcgca acggtcaagc agtacgatgc caacagccag ggctggatgg aacttcagag
      721 caatacctgc gatgccgtca tcatcaacac gtcggtagcc ctctactatc tgaaagaagg
      781 cggcgacaag gacctcaaaa tcgtcggtga ttccaagaaa accgatgccg gtattgccat
      841 ggctgtcgga aaagataaaa cagacctcct cgaaaaagtc aataaggcct tggctgacct
      901 caaagcagac ggaacgtatg cgaaactcta caaaaaatgg ttcggcgtcg acccgcagat
      961 gtaatccatg tataagacat atatcttcga cttcgactac actttggcca actcggaaaa
     1021 gggtatcgtc atgtgcttcc agctcctctt tgaagccgaa ggctatgaag gcattcccga
     1081 agacgccatt cgccgtacga tcggcatgac catgtacgat gccatgaaaa gccttaccgg
     1141 cgaaacggac ccggaccgga tctatgaact gattaaggaa tataaagtcc gcttctccga
     1201 caagtatatg acggccaata cccatctcta tccagagacc ctgccggttt taaaggccct
     1261 gaaggaaagg ggaagccgct gctgtatcgt atcgaccaag acccgttcgc gcattaacga
     1321 aacgctccgt aaatacgcca tggagtccct catcgacctc gtcgtcggca tggaagacgt
     1381 ggacaatgca aaacctgcac cggatggaat caatcgggtc tgcgaccatt tcgggctgga
     1441 taaaaaagac gtcctttaca tcggcgacaa taccatcgat gccaaggctg cacaaaatgc
     1501 aggcgtcgcc tttgctgccg taacgacagg aacgacatcg gagccggatt tccttcccct
     1561 gccccatgtg gcaatcatga aagacctcag cgatatcctt gatattccct aattggagga
     1621 acctactatg atgaagaaaa aagtgatgac cttggcagcg gcttcgatag ctgccttgac
     1681 cttactcgcg ggctgcggcg gcagcacaga acagtccgcc ggtgcgaagg gagacaagac
     1741 actcatcgtc gggacggaac cgaccttccc gccttttgaa tttacggaaa atgataagga
     1801 cgtcggcttc gatatcgatt tagcccaggc aatctgcgat aagttaggct acaagatgga
     1861 agtcaagaac ctcggcttcg acgccttgat tccggccttg aagagcggcc agattgacat
     1921 catcgccgcc ggcatggatg cgacggaaga acggaagaaa cagatcaact tcacggacgt
     1981 ctactatcag ggcggctaca ccatcgtcgt tcccgtaggc aatacggata ttaccggtta
     2041 tgattccatc gccggaaaga ccgtcggcgc ccaggtcggc tcaaaagcgg ccgactatgc
     2101 ccgtgaacac ggtgccaatg tcaaggagtt cgacaccaat acacaaggct ggatggaact
     2161 cgaagccggc acatgtgacg ccgtctctat cgaccaggcc gtcgcccagt actatcttca
     2221 gcagggcggc aaagacaagc tcaaactcgt cggtgctccg atcacctctc gcggtgtagc
     2281 catggggatc agtaaagaca aacaggaact gactgaccag gtcaaccagg ccttaaaaga
     2341 actcaaggca gacggaacgt acgacaaaat ctatcagaaa tggtttggca cagcaccgaa
     2401 taaataaagc gtaaagtctc tattcattcg aacggtgtcg accgtcaaca gcataagaaa
     2461 acagccatcc atcgggatgg ctgttttctt atgcttactt tttctgtggc tgctgcagct
     2521 ttgccatgcg gtccttcaga gacgtaaagg gcgtctgatt gtcagactgg agttcttcag
     2581 gctgaatcgt cgtatcacga cgagcagctt tccgtctgga ttcggcttcg gcaacccgcc
     2641 gttcttctgc ttcacgcgag ctctgtacgg cttcgcgaat aggctgaagc ttactatccg
     2701 gtacgaccag gaagcgaacc ggcaagttgg ccgcgcctgg cggcatgatg tgaatggtca
     2761 tatccgtccc ggccgggaag gtatccacgt attccatggc ataagggtca ttgagtccca
     2821 tagaatgacg atgacgaggc aattccacga tttttttatc ttctccgcgc tgaggatcgc
     2881 tgtagataag ctctgtaaca ccggcatatt caccgccttg gggattaaaa tagaggtgaa
     2941 tgtctccggt cccttgggta cgcagatgaa tggtgtaatc gaggccatag tttcccgtat
     3001 cttgcgacga gcgattatcc atgacgtccc gtccttgcag aaagcggtcc atgacaccgt
     3061 ccgccaagag gatatagccg atgccgctgt ccgtagagta aggcgtaagg gtgctcatga
     3121 agcggtcttt gccgtgaaaa gttccccgca gtttgacgga gtcactgggc aggtactgaa
     3181 gcttacggac aaaggctgtc ggatcttctt taaccggcaa aatcatggac gacagaatca
     3241 tcggttccgg aaggctgacg ttgatgatgc ctgaaaagag ctgatccgga tggacgacga
     3301 cttcattgag ccgttgtccg actaaggcat aatcatgagg ctgtaccgta accttattga
     3361 ccgtcatatt cccttcataa tacagggtcg acagttcctt gccgacctga taataatcgt
     3421 tactgggacg cgtatattga tagccttgaa ggaaggcgtc aatcggatag tcatggggat
     3481 tatacagcat ggctacgatt ttgcggtctg tcggctgctg attgacatga taaaagtaaa
     3541 ggcgtacatc accgctgacc atgtccccat agagaatgcc gtcgttttcg gcatattccg
     3601 gactgtcaga taagagcagc gtccccgtat caataggcgg cgtcatcgaa atggacttca
     3661 tcggttgaac gacaacccgg ctcatgacct ctgaatcgtc gattcccgga aatccggccg
     3721 ccaatggggt ccccataggg ataaaggccg tcaaaagacc ggcaatataa tcgatgagtt
     3781 aatccggact ttgtcaatcc tgactgagcc gccaattcgc tcatcgaagc ctccggattg
     3841 gccatgcgca gttctgccgc ttcgcgaagc ttagccgaca tacgctgata aagtcccgat
     3901 tcctgaatac gggtaatagc ctggcgctgg cgaacagcgg catcgaccgt tttctgaaga
     3961 ttcgccgttt cgcagttgac gacacgattg acgttgtttc gcatctcctt tacgacacga
     4021 acttcttcaa aggtaagata ggcttgggca gccccgatat ac
//
LOCUS       DAWDXZ010000106         4027 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5438_length_4027_cov_8.323515, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000106 DAWDXZ010000000
VERSION     DAWDXZ010000106.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5438_length_4027_cov_8.323515"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    804..813
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          964 a         1071 c         1049 g          933 t
ORIGIN      
        1 aaatacagtc acattggcta tgccgtattg gagtgtgaat catggaaaaa gtaattgcca
       61 tcatcggccc gacggcggtg ggaaagacag ccctcagctt ttccctggcc gatcactacg
      121 ggaccgacct catttccggc gatgcctatc aaatttatcg ccacatggat atcggcacgg
      181 ccaagccaac ggccgatgaa ctgacccggt accgccatta tttaatcgat atcgccgacc
      241 ctgatgagcc gtattcggct gctaaattct gctgcatggc aggtgaggct attaccaaaa
      301 tcaatgggga ggagaagatt cccatcctcg tcggcggcac gggcctttac gtccagtccc
      361 tcctcgaagg ctacgacttt caggcctcgc ggatgacgga agccgataaa cggcgagctc
      421 aggccaagtt agaagggctc gatgaggaaa ccctcaaaga ctatatccga aaagaaacgg
      481 attgggagcc gccggactgg cacgaactct tgtccaacac ccaccgcttg acgcggctgg
      541 tcagcgctgt cgaacagggc gagggaaaat ccttcgtccg ccagggcaag gcccgtgatc
      601 tcgtctatga cgcctatgtc atcggccttc gcctgcctcg ggaagttttg tacgcccgta
      661 tcgaagaacg agtcgacatc atgatagaag gcggttggat tgacgaagtc cgccatctcc
      721 tgaaccttgg tttttccctt gactctcagg ccatgaaggc tatcggctat caggaattgg
      781 gccgatacgt acagggtgcg atgnnnnnnn nnngtctctg gatttagaag tattacgaaa
      841 agaagcactg gcactttgtg cccctcagtt tgaacgcatt gataccattt gtctgaaaaa
      901 cacggaaaaa gtattgaaag cctataagga tgcgcaaatt tcttcctacc agtttgccgg
      961 cacatcgggt tacggctaca gcgacatggg ccgggaaaaa ctcgatgaag tctttgcccg
     1021 ggtattcaag gcggaaaaag ccttggtccg tcctcacttc gtgtcgggaa cccacgcctt
     1081 ggcgacggtc ttactgtctc tccttcatac gggagacctc atgatgagcc tcatcggcgc
     1141 tccttacgac acgatgcagg gcgtcatcgg ctatgcccgc catacgcctc attccttgaa
     1201 agaaatcggc gttctctatg aagaggtacc tttaaaggac aattcttacg acctttccgg
     1261 cattgaaaaa caggttggcg aaaagcagcc gaaactggtc ctcatccagc gttctcgcgg
     1321 ctacagtacg cgggcgtcgc tcaccattga agacattgaa aagatcatcg ccgtcgtccg
     1381 caaggtcagt ccgaagacca tctgcttcgt cgataactgc tatggtgaat ttgcggccga
     1441 acgagaacct atcgaagccg gtgccgatat catcgccggt tcactcatca agaatcccgg
     1501 cggcggcatt gctccgacgg gaggttatat tgccggcaag gcggaactcg tcgaagctgc
     1561 agccgactac ctgacggcac cgggactcgg tgacgaactc ggttcttatg ctgccggcta
     1621 ccgcctcttc ttccagggat tttttatggc ccctcacgtt acggctcagg ccattaaagg
     1681 ggctgtcttt gcagccgctg tctttgaaaa aatgggcttt cacgtatcgc cggccgttac
     1741 gacgccgcgc gtcgatttga ttcagactat ctatttagaa aatgagcaga atatgtgcct
     1801 cttttgccag ggaattcaag gcttttcgcc tgtcgatgcc catgtcacgc ccattcctga
     1861 tgatatgccg ggctatgccg acaaaatcat catggcagcc ggcgacttcg tccagggctc
     1921 gtccattgag ctctcggccg acggcccgat tcgcgatccc tacatgatct acctccaagg
     1981 gggcatcgtc tttgaacaca acgtattggc tatcttaagc gcggctcagt acatcgccga
     2041 acatcgggaa ggagcggctc atgattaagg aagtcctggt cgtcgaagga aggtctgacg
     2101 tcgcccgcat tcgggccagc cacatcgacg tcgatatgat cacgaccgat gggttcaact
     2161 taaaacccca tacgctggac cagattcgct gtgcttatga aaagcgaggc attattatcc
     2221 tgaccgaccc cgaccgggcg ggggaacaga tccgaaagtt cctgaccgat cgtttcccca
     2281 atgccaagca cgcctttatt ccccggaaag atgctattgc caacgatgac ctcggtgtcg
     2341 aacaggcatc acctgaagcc atccgcctgg ccttgtccaa ggttcgctgt gccgcctatg
     2401 aaccgcagga taccttttcc atgagcgacc ttaacggggc cggattgaac ggcagtcctg
     2461 acgcatcgga ccgccgggcg caagtcgggg ccatactcgg catcggctac ggcaatgcca
     2521 aacagttttt aaaacgatta aatcattacg gtgttacccg ccaggagtgg gaccaggcta
     2581 tccgccagat tgatgaggtg aaataatgaa agaagtcgac ttatctaatc ccgacgtcgt
     2641 ccgctacgtc gtcaaccgct ttggcctgcg catgaataag aagctggggc agaacttcct
     2701 cattcgtcac agtgtcgtcg atgacatcgc cgatgcggcg gatctcagtg agggcgatcc
     2761 ggtccttgaa atcgggccgg gcatcgggac cctgacccag gcacttgccg aaacaggggc
     2821 agccgtaacc gctgtcgaat tggatgacca tctgctgcct gtcctcgata aaaccttgga
     2881 acattacgac aacgttcgtg tcgtccacgg cgatatcatg cgtacggaca ttgaaaccct
     2941 catgaatcac aagcctttca aggtctgtgc caacctgccg tactacatca cgacgccgat
     3001 catcatgaag ctcttggaac agaaactgcc aattgaacgg atcgtcgtca tggttcaaaa
     3061 agaagtggct gaacgaatgg tagccgtacc gggccataag atctacgggg ctctctcggt
     3121 gtcggttcag tattatacgg aaccgaagat gctctttgaa atctcgccga agtgcttcat
     3181 gccggcgccg gaagtcacgt cggctgtcgt tgccatggat gtccgtaggg aacctcctgt
     3241 cgatttgatg gatgaaaaac gcttctttag tgtcgtcaaa gcggcctttc agcagcggcg
     3301 gaaaaccttg tccaatgctt tgaagaatac ggggatgacc aaggaacaga ttgccaatgt
     3361 cttggaagcg gcctccatcg acggaaagcg tcgtggggaa accttgtcgc tccaagaatt
     3421 tgccgacatt gccaactact gggtcaaaac gacgaagagt gagaccttgt aagcgaagga
     3481 gcgccaggac cgatcgccgt gccttgcgtt tgtgagagga ctccctatga atggagtata
     3541 gcgaaacctc cttcataggg agcaggcctc ttccacccca aggaagcgtg ctggcaaaga
     3601 tgttttaaaa gagcggtttt tctgtcgacg atgaggaact tacctggaaa ttacttgacg
     3661 aatagtatgt accgtgatat actaattaag tcttttgtgg gttcgaatcc cacccgctcc
     3721 gccacaataa aaagccgcac tgttaagcct ttataggttt gccagtgcgg ctttcttttt
     3781 ttgtcatgaa ataaatgatt gtcttatcgt tggatgtttg tcgagagaga gaccgatggt
     3841 attgtaatcg ccttcacata attttacgaa gagcaagaaa cagccgtgag ttttaagact
     3901 cacggttgtt tcgattaatc tgactgtcgc cctcgtcatc atccggcatc gatggagtct
     3961 cctggcgata ccatgtgggt gtgttttacg cgccaattcg tcattttatt gtaaagccag
     4021 agaccat
//
LOCUS       DAWDXZ010000107         4011 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5456_length_4011_cov_7.152427, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000107 DAWDXZ010000000
VERSION     DAWDXZ010000107.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5456_length_4011_cov_7.152427"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          951 a         1068 c          977 g         1015 t
ORIGIN      
        1 ttacgttcga gattcgatgc gtcgagaacg ttgacgatga tatccggctt ttcattgatg
       61 atgacgttac gggcaaccac ttcatcgagg gaccgcgccg tcaagctata cgtacccgga
      121 aggtcgacga agagcaaatc cttgccgtta aaggtgcgat ggccttcctt cttttcgaca
      181 gtaacgccgg gatagttgcc gacgtgctgc tttgaacctg taatgttatt aaaaatcgtc
      241 gttttcccgc agttaggatt gccggcaagg gcaatcttaa tctttcctgc attttccatg
      301 actactaaat acccccttat tattgatcca cggctaccga aatcatagcc gcttcattca
      361 tgcgcaaggc catagcacag cctttaattt tgacttctac cggatctccg agaggggcct
      421 ttttaaaaac agaaatacga gaacctttga cgacacccat atctaccagg cgatgttgga
      481 tatttccccg gccttcaagg gcttcgacaa tacctgactg accgactttc atgtcagcta
      541 acgaaacaat cttcatgact aactcccctt tgcttgccta gacgacggct tcctccgtcc
      601 tggcggctag tatagttttc agtgcacttg agctgctggt atgggaacgg acaatatcca
      661 cgttcttgcg ctgggcttcc ttcagagcgc agcggaccat actgtggctc acggtactgg
      721 taaacaacac cacgagatca ggcgacccga ttttcgactt aaaatcactg ggtacctgag
      781 taaatacttt ggccttataa tcgaattctt tgcaaatacg tttataacgg cacaccatgc
      841 aatcatttcc acctacgatg acgacactca tttacatcgc tcctttcagc tccgtaaata
      901 atactcacac atcccttcaa cgaattataa tatatttcat tacaaaatat attatatatt
      961 ttttcacata ctctgtcaac acattgctat tgaaattcat ttataaaaag aaagaagcgc
     1021 acgaaagtgc gcttcttatc tttacaaaat gcaccttgat gcatcctttt gaatatgtga
     1081 ttatatatcc taattaagac ttattctcta aaattaagac tttttaggct cgccgtttcg
     1141 gcatggatgg cccgagaatg cgaacactat tcaatacgac agccaaggtg gccgtattgt
     1201 ggatgaccgc ggcccagagg gggcttatct tccctaaggc accgagaagc atggctgccg
     1261 agtttacagc aatggtcgcc gtaaagttct ggttaatggc ccgcatcgtt tcccgtccga
     1321 tatggagggc atcatataac ttagccgggt cttccgaacg gattgtaatg gctgccgatt
     1381 cagcagcaat atcggttttg tttccgccga ggctgacacc gatatcggag aaagccaggg
     1441 ccggcgcatc gttgatgccg tcgccaacca tcatgacgtt gcctcttttt tgcatcttca
     1501 tgacgtagtt agccttttct tccggcagga tttcagcata atacgagtcg atatccatat
     1561 cctgggcgac ttcggcggcg acgtctttcg agtcgccggt cagcatgacc acttcatcga
     1621 tgccgaggcg gcgcatgcgg ttcaaggtct tcttcatgcc cggacgaatc ggatcctgaa
     1681 tggtcaagaa gccgaggtac tggccatttt tagcgatgta caacagattc ttgccccagg
     1741 tcttattatc gacgaccggc atgcctccta cgccctcttc ggtcatgaag cggcggctgc
     1801 cgacaaggat atccccgcct tcgtagtttt caaacggcgg tacgacagcc ttcatgcccc
     1861 tagcaacgac cgtttctgac gaagcatgtg gcggtgtctg ccagcccttg gcattgacgt
     1921 acttctgtac ggctacagcc agaggatgta cggaatgcat ttcagccgat gcggccaaca
     1981 gtatcatttc tttatcgccg acgccgtcaa tcgtttcgat atggtcgatc tgggggacgc
     2041 ccatggtaat ggtcccggtc ttatcgagga cgaccgtatc gatatcggct aagttttcga
     2101 tatagttgcc gcccttgatg aggatccctt tccgagcggc gacgccgatg gctgccgaaa
     2161 tggccgtagc cgtcgaaagt ttcaggccac acgagaaatc gatgaacagc aaattgagaa
     2221 ctctttgcca atctttggtc gccccgtaga cgatggctgc cccgataaag gagatcggca
     2281 ctaagagatt ggccatctta tcggcaaagt tctggaccgg cgcccggcgg gtctgggctt
     2341 cttcgacgag atggatgatc tgagccaggg acgtatcggc gccgaccttt tcgacgcgga
     2401 tgacgaggtc tccggcttca atgacactgc cggcatagac cggcgacttt tcctgtttga
     2461 tagccggatt cgattcgccg gtaatggaag cctgattgac agcggcgttg ccgtcgacaa
     2521 cggtaccgtc gacgcagatc ttttcgccga gatgaacggc aatgaggtcg ccccgcttca
     2581 gtgattctac gggaaccttg cgttccctgc cgttttcgat gatccagacg tcgcgctgat
     2641 ccagtttcaa aaggccggaa atctgacggc gtgcccgttc ggcagcgtat tcggtcatca
     2701 tttcagcccc gtttgacagg gccaggagcg tcagactcga ttccggcttt ccggacagga
     2761 ccgaggctgc taccgccgtt gccgtcaagg tatcggcgtt ggcctgatgg tcttttacca
     2821 ggccgaggat accgctttta atatagcgac gcgcgatgta taaggtaaag agtcgacgaa
     2881 acaagagcat ggatccgtaa aattccggat tgaccttgcg caggatgttc atagcaatga
     2941 aaccgccgac agagatgatg gcttcccgtc ggtacgtggc aacgtccatg tcttccggtt
     3001 tattttcctt tttccggccg tacttatctt caaaagcccg aatgaacagt ttcagcgccc
     3061 gtacatccag cgttttacgg taatagacgg taaccttttt ttcttcgaca acggccgaga
     3121 cgatatcccg cacctgacga agcttgacgc tcaggaccgg acgcatgcgg ctgggaattt
     3181 cgctgtgaag aaaaatcgta ttgcacaaat acatgattat tccctcctca agagcgaaag
     3241 tgcccaccac agcatctgcg gcccggatgg acgatcgccc atgaatactg ttttgcgaag
     3301 tccgcggata accaggaaca aggcagctaa ggtcgtcaaa tcgagaagat gcttcgtttt
     3361 tcggcgaatg accttattga agaaatcccc cgtcgctcga gctaccttga ggtacgccgt
     3421 ttcatccgcc cggcccgatc ctttgcggtc ggcatcatag acggcattga taatcccttc
     3481 aacggcgctg ggaaagaggc gggaacggaa gaaatcttcc aaaagatcca gggcagcttc
     3541 acttactgca tagaccaaaa tactgcccgt aacggtgttg acctcgatgg aactgatacc
     3601 ggtcatgaca ccgattttct tttccatgaa ttcggccaac cctgcattgc cgacaaggga
     3661 cgcagcccga tagcgacgac ggcccgggag tgcggaaacg caggcaaacc gcggcacatc
     3721 agctgccttt cctccttgca tcgaaggcgc accggcaaag gcgctgcctc cctttttctt
     3781 attatggaac aagtcgtaaa cggcctttcc gatcgacgtg cccaccataa aacctgaggc
     3841 gtatgacata atcaccttct cctttcagca tgggtcatga tatagttttc tacccgaacg
     3901 agttctcggt ttgtccgcag gacctgcggc cgataggtaa tcagtaccga acccgtaagg
     3961 acgttgatat cgacgttatc gatttcttta tagctggcaa tataggcgta t
//
LOCUS       DAWDXZ010000108         4008 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5460_length_4008_cov_8.730331, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000108 DAWDXZ010000000
VERSION     DAWDXZ010000108.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5460_length_4008_cov_8.730331"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1650..1659
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2110..2119
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2803..2812
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          944 a         1066 c         1131 g          837 t
ORIGIN      
        1 cgaccatggt gacgtcttcg gcaccggccc ggaccaagag gcggccgatg gaagctccgg
       61 cagcaccggc accgttgatg acgatgcggg ccgtcttgag gtcctttttg acgaagcgca
      121 gggctccaat gacgccggct aaggcggcga tggccgtacc gtgctggtcg tcatggaaga
      181 cggggatgtc catttcgcgc ttcagcgttt cttcgatgtc atagcatttc ggagatgaaa
      241 tgtcttccaa gttgataccg gcaaaagacg gctgcaggcg cttgaccgtt tcgatgaact
      301 ccttcggatc cttcgtgtcc aggcagatgg gaacggcatt gacgccgccg aataccttga
      361 acaggataga tttcccttcc atgaccggca tggccgcttc cgggccgatg tcgcccaaac
      421 cgaggacgcg ggtcccgtcc gatacgacgg caatcatatt gccccggcag gtcaggtcaa
      481 aggacagatc cggatctttg gtgatctccc ggcacggctg ggcaacgccc ggcgtgtaga
      541 gtacactcaa atcgtcgttg gtctgtaaga cggcccgagg gaaggtttcg agaatcccct
      601 gtttacctcg gcggatcttc atggcttgtt catcaaaatt catgtcaaaa aactcctcta
      661 caatagttta tgcgttaaaa atagcttgcg gttctgtaaa ttcgagcatc ccgtctttgc
      721 cgccttcacg gccgagaccg ctgtccttaa agccgccaaa aggcgctgtg acgtcgcgcg
      781 ggctgccgtt gacgtagacg ttgcccgaca agatgccttc ggcaaccttc gtcgcttctt
      841 ccttatcggg accccagacg gcaccgttga ggccgtactt ggtatcgttg gcaatggcca
      901 gggcctggtc gacactgtca taagtgagga cgcagaggac gggcccgaag atttcttctt
      961 gggcaatggt catgtcgttg gtcacatcgg taaagaccgt cggattgatg aagtacccct
     1021 tttcaggcgc ttccggacgg ccgccggcca ggaccgtcgc cccttcttcg atgcctttgg
     1081 caatgtacga atcgacgatc ttccactgtt ctgccgaagc gacagcgccg actttcgtcg
     1141 tttccaaggt cgggtcgccg accttatatt ccggcaggac cttgaggatg atgtcttcga
     1201 tttctttttt cgcttccttc gggatgatca gccgcgacag agccgtgcag gtctggccgc
     1261 tgttgagcag gagcgaattg aagcacatgc ggacggcctt ttcatagtcg gccccgggca
     1321 ggatgataaa gggtgatttg ccgcccaatt ccatgctgat gcgcttcata gaagccaagg
     1381 cccgctggga gaccttgata ccggctttgg tcgaacccgt aaaggatatc atggcgatat
     1441 tggggtcgtc gcagatagcg ttgccgattt caccgctgcg gccggggatg aagttgaaga
     1501 cgcccttcgg gaagccggcc cgatcaaagg cttcggccaa ataataagcc gttaccggcg
     1561 tagactggct gggctttaag acgaccgtag caccgactaa aatagccggg atgactttct
     1621 ggataacctg gcccaaagga tagttccacn nnnnnnnnng cgtgatgcag ccgatgaccc
     1681 ctaagggacg gcggtagacg acggactggg gcagtttttc gactaagggc aggtccgggg
     1741 ccaagtcgat ataagactgg gtccgtacga actggtaggc gatgtgattg gtcttaacga
     1801 aggaaacggg gatgcccaat tccttgacgg tggcatcgac catctgcggt tcaaactgaa
     1861 tgagctcttt aagcaatttt ctcataaaat caattcgttc ttccatcggc cttgccgccc
     1921 acgcaggaaa ggccttggct gcagcagctg aggccgcggc gacatcctgt tcaccggcag
     1981 cggcaacggt cgtgaaaact tccttcgtcg ccggattttc aacgtcgatg tattggccgg
     2041 ataagggagc tacccactgg ccatcgatat acaatttttt aaagtccatg aaaaaaacct
     2101 cctttgcgcn nnnnnnnnng atttatgcta ccattataga tagaaattct ccattgtgca
     2161 acaaaaaagg catcgtactc tgaaaaattt aagtacaatg cctttaccat tattttgcct
     2221 cgacttccaa ggcctccgat tccgacgcga aatgccccag aacagacccg ccgacaagac
     2281 cgacgacaaa gggaacgatc cagccgaagc cgtaggccga caagggaacg gcagcgacga
     2341 tggctttggc aacggccagg ctgctaaggc ccggcaggga ttccaagcag gcgaagaccg
     2401 tgaccaagta gaccgaaccc ttataggccc actgattggg cagcaaggga ccgacgatgc
     2461 ctaagaccaa taaggccagg cacggcggat aagcgacgcc gaagatccag ccgatgtagg
     2521 tgacgatgcc gtcgacaccg aggaaggccg tcagggccga gaggatcgag caggcgacgg
     2581 ccgcctgttt ataactgaca cggctgccgg aagtcgtgct ggtaaattcc gcaacggcag
     2641 cgatctgacc gatggctgtc gtcaggcagg ccaagacgac ggcgatgccc atgggaatca
     2701 tgacccaggt gccgatgcgg tggatgatgg ctaccaagag ttcagcccgg ccgatggact
     2761 ggggttagta ggaactgacg ctggccccca tgtagagcag gcnnnnnnnn nnaggctgcc
     2821 gtagatgatg agcagcagga cgaaggagac gacgccgcag tagatgagga ccttattgcg
     2881 ctggccgacg cccgtatagc ccttgccgcg aatggagctg aggaagacgg cagccatgat
     2941 gaagctgacc aggacgtcgc ccgtattgta gccgccgagg aaggcattga cgaagggatt
     3001 gtctgcggca ggttccgccg gcgtccccaa ggggaagaaa atcccggcaa tggcgatggc
     3061 caccaaaatg aaggccaagg ccggcgtcaa atacttgccc atgcggtcca tgacgttatg
     3121 cgggtcggcg acgaagaagt agcagagcag gaaataaagg acgactaaag gaatcatggg
     3181 cgctgccgga aaatttcctt gtacaccgag ctggatggcc gtcgccgccg tccgcggaat
     3241 ggacacgaag gtacagacca gcatcaaagc cgccaataaa atccggtacg tatattttcc
     3301 gaccttgtcc gtaatcgtat ggacgtcgcc gttgaggccg atgacgatga gcgtaaaaat
     3361 aggcaggaaa atccccgaca aggtcaaacc ggccaggccc gacaagaaat cactgccgct
     3421 gttgagcccc aagtgcggcg gaaaaataag attgccggcc ccgaaatagg tggcaaaaaa
     3481 agcaaagccc aggatgatga catcccgttt ccgaatcgtc tgtctgctgg aatcattcat
     3541 aagaaaagtc cccccaaccc catcataaag aataagtgca tcatacaaaa aagaggcgct
     3601 gccctcaagc tgcgcctcaa ttcacatcat atctttcagg aatagcctgt aggaattggc
     3661 accttaccat tggcaggttg ccgcagcgtc atcgggccca tccctcagct gctctggata
     3721 ttgcggtatg aaattttgta tgtattggta gtattttata aaacttttcg ctgtttgtca
     3781 ataacttttt aacaaaatta cttcttcgac ggcttatttt gacttcccgg gcataaatca
     3841 gagaggaagc attcgtcgca taaaggcttt cgggccttgc agacccgtcg tccgtgccag
     3901 atgagccaat gatgagcccg gctccaccat tccttgggaa tggccttctg cagtcccttt
     3961 tcgacttcaa ggggcgtctt gcctttggcc agctggagcc gattggat
//
LOCUS       DAWDXZ010000109         3994 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5476_length_3994_cov_9.919523, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000109 DAWDXZ010000000
VERSION     DAWDXZ010000109.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5476_length_3994_cov_9.919523"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1515..1528
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2567..2576
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3028..3037
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1004 a          903 c          996 g         1057 t
ORIGIN      
        1 atggttttta tatagaagga gagaccccat tatgaaaaag aaccttttta aacaagtatt
       61 atcggcagcc gtattgacca tgtccctggc cggcggtgcc gccttcgttt cggccgattc
      121 cgtacagttc agtgaaaacg gtgactatca ggccgctgtc aatttaagtg taccggctgt
      181 ttctaagagt gatgatattc gcggttacag cgaaaacaga gattataatg ccgccattaa
      241 cctgagcgta ccggcagctg taaagagcat cgattccgtc cagtacagcg aaaatcgtga
      301 ctacgttgca gcttcggctt ttgacaacaa ctaatcgatt tacagtaaga ctttcttgat
      361 tgacgaagac atttcaatca cttcatctcg taatagcgag ttacagatag attcagaaaa
      421 ttccaaccat gtgcatgaca atggttttta tatagaagga gatagattat gatgaagaat
      481 atgtttaaac aagtattatc ggctatggta ttgaccatgt ccttggccgg aggtgctgcc
      541 ttcgtttcgg ccgattctgt acagttcagt gaaaacggcg actatcaggc cgctgtcaat
      601 ctgagtgtac cggctgttag caagacagct gacttccgcg gctacagtga aaaccgtgat
      661 tatgacgccg cgattaacct cgatgtcccg gcagctgtaa agagcatcga ttccgtacag
      721 tacagcgaaa atcgtgacta cgttgcagct tcggcttttg acaacaacta atcgatttac
      781 agtaagactt tcttgattga cgaagacatt tcaatcactt catctcgtaa tagcgagtta
      841 cagatagatt cagaaaattc caaccatgtg catgacaatg gtttttatat agaaggagat
      901 agattatgat gaagaatatg tttaaacaag tattatcggc tatggtattg accatgtcct
      961 tggccggagg tgctgccttc gtttcggccg attctgtaca gttcagtgaa aacggcgact
     1021 atcaggccgc tgtcaatctg agtgtaccgg ctgttagcaa gacagctgac ttccgcggct
     1081 acagtgaaaa ccgtgattat gacgccgcga ttaacctgag cgtaccggca gctgtaaaga
     1141 gcgtcgattc cgtacagtac agcgaaaatc gtgactacgt cgcagcttct gctttcgaca
     1201 acaactaata acatcgcaac aacgatgtgt agatagatta agaaaattcc aaccatttgg
     1261 caaagccctt ggtttttatg tagaaggaga taaatcaaag agtgtcgatt ccgttcagta
     1321 cagtgaaaat cgtgacttcg atgccgctcc agcttttgac aattaataac agatagtgta
     1381 cagttagggt gcacctttga atttattata accatcaggc tcagcccttg gttttgatat
     1441 acagcatcag gctgtttgtg ccgtagaaat gatgctcctt attgaggata acttatggtt
     1501 tttcgtaagg tacannnnnn nnnnnnnntg gcggcggttc cattaaaaag aacggcgtct
     1561 accaagatat tctggatatt ttgcagaaaa atggtaagaa ggtcgctgaa atttccgggg
     1621 ttatgcccaa tccgacgctt gaaaagttat acgaaggtat tgaaattgcc cgcagccatc
     1681 aggcggattt cctcttggcc gtcggcggcg gttctgtctg tgactacacg aaggccgtag
     1741 ccgtttcggt ccattgcgac gaagatccct gggataaata ctttatccgc tttgaagaac
     1801 cgacctgtcc tatcgttccc ctcggctgtg tgctcactat ggccggcacg ggctcggaaa
     1861 tgaacgccgg ctcggtcatt accaacagtg aaacgaagca gaaaatcggc catgttttcg
     1921 ccagctccga cgtcatgccg aaattctcca tcctgaatcc gcggtatacc atgaccgtac
     1981 cggattatca gatgaaggcc ggtatgttcg atatcttcag ccatatctgt gaacagtatt
     2041 tctccggcac cgacgatgtg accagtgatt acctcagcga aggcctgctc cgttcagtca
     2101 tccactccag ccgcattgcc gtcaaggatg ctcaggatta tgaagcccgc agcaatatca
     2161 tgtggtcggc tacgtgggcc ttgaatacgc tcttgtcctg cggcaagccc ggtgactgga
     2221 tggtccatat gatgggccag gctgtcggcg gctataccaa tgcaacgcac ggcatgacct
     2281 tgtccgccgt ttccctgccg tattaccgct atatccttcc gtacggcctg ccgaagttta
     2341 aacgctttgc tgaaaatgta tggggtgtac agcctgaagg caaaacggat gaacagattg
     2401 cccgcgaagg cctcgaccgg atggaagact ggatgaagga aatcggcgtc gtcttgtcca
     2461 ttaaggatct gggtgctgac gaatcgatgc tcgacggcat tgccgacgtc accatgattc
     2521 ttcgtggcgg ctataaaaag cttgaccgcc aggagctcat tgatatnnnn nnnnnnaatc
     2581 gtaaggcata acaagtacga tccgtatgat agattaaaaa agggttcgca tgattgtttc
     2641 atgcgaaccc tttctggacg tcttcacttc gttgtgtctt gtaccagact ttggcctatt
     2701 tcataggcct ctttcagaca gatggggccg gctttttctt ggtaggcttt tttgtcgctt
     2761 tccgagaaga ccgacgattc atatttcgag taatcgttga actgcagcgt atcgtaggcc
     2821 caaagggtct tcggcggaat gccgaggatg cggtgggcga agtcttcctg ggcttctacg
     2881 ttttggggca ggtggaattc tttcgcctgt tcttccgtga cgttcatggt gtagataaag
     2941 gctgatggaa tctttttgcc gaagacggac gggatttctg cgctgtaaat atagtttgag
     3001 aagaagagcc gttctaaaaa ggcatgcnnn nnnnnnnatg catgccggac gtgaggctca
     3061 taaagtagat gggcgagccg aagaggaggg catctgcttc tttagccttc gtgaggaaag
     3121 gcgacaagtc gtcttttacg atgcacgtgc cgtggtcctt gtcttttcgc ttacagtaaa
     3181 aacaactgat acagcctttg taattcaagg aatagaggtc gacgtattct gtgtcggctc
     3241 cagaagctgc ggctccccgc agggcttctt tcaatagttt tgccgtgttg ccattgcggc
     3301 ggggactgcc gttaaaggcc ataattttca tcattcatca tcctttcttt taaatgttat
     3361 atcattatat cgcgttatat agatttattc aagaggaaac ctatcgattt aatgcatggc
     3421 gggggctgaa tctccgcgta tcttgtcgtt actgtataaa tagggggaat tatcatgacg
     3481 aaaaaaaaca tcgtagctat cttggcagcc gcctttatgg ctgttagctt atcggcctgc
     3541 agcagtgtcc gcactcaggc tgacggccaa tccggggcta cggaacagac gtcgcagacg
     3601 gcggcaaaac ctaaggtcga tgccgatgcc agtgcatcca tcaagggcgc tgcatcggat
     3661 cagaaagaag gacagcatac ggacagccgt cgggccgtca tttatttttc ttctcctgac
     3721 agcagcgaca tgaatgcctt ggaagggagc acgcagtatt tgggccgagt cattgctgaa
     3781 aagaccgggg ctgacgtctt ccgtatcgta ccgatcaagg cttatccgac cgattacagt
     3841 gcccatacgg ctcaggctaa gatggaacag ggggaaaatg cccgtccggc cattcaggat
     3901 aatttcggtg acctcagtca gtatgacacg attttcttag gctatccgat ttggtgggga
     3961 gatctgccta tgccggtcta taccttcctg gaaa
//
LOCUS       DAWDXZ010000110         3983 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5495_length_3983_cov_8.446283, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000110 DAWDXZ010000000
VERSION     DAWDXZ010000110.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5495_length_3983_cov_8.446283"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    879..888
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          943 a          997 c          990 g         1043 t
ORIGIN      
        1 tatcctatcg ctgggtttct ttaagatgcc gggctttaag gcctgtccca ttgccttggt
       61 acttgcctta gccgtggcac tgacggcgta tcaaatggat gggcaagatg ccgcttcggc
      121 ggccttagaa ggcgtggccc tggcgtgctg gcctatttta ttggttatca tcgcggcgat
      181 ttttacgtat aatctcgccg tacatacgaa gagtatggat attatcaaac atatgcttac
      241 gacagtcagc aaggaccgcc gcatcctgac ccttctcatt gcctggggtt tcggtgcctt
      301 catggaaggc atggccggtt tcggtacggc cattgcgatt ccggcgtcga tgttggcggc
      361 ccttggcttc aatcctatta agtccattct cgtctgttta gtcgctaatt ccgttcctac
      421 gacgtatggc tctattggga ttccggccag taccctggcc aatctgaccg gtctcgatcc
      481 cattcggatt tcttcctata ttagcctgca gctgctgccg ctcaatctga tttgtccttt
      541 tctcatcgtc cttattatcg gcggatccct gaaagccttg aaaggcgtct tcctgattac
      601 gctgctgtcc ggactggctt tagccgtacc ggagctcatc atttcatcct ttgtcggtcc
      661 tgagctggcc gtcatggggt catcggtcgt cattatggcc gtcattatct tgtattcccg
      721 gtttcaatcg agtgaggcac cggaatacac catggatatc catccggccc atgtcccgct
      781 taaagggggg ctgctggcct gtctgccttt tattctgatc tttatctttc tgctgatgac
      841 gtcgaagctc gtgcccttca tatatggccc actcatggnn nnnnnnnnaa tcgtcggtct
      901 cgatttatac cggtgaaggc ggcgttccct atacctttgt atgggtgaat acgccgggga
      961 ttctcatcct attggcagcc tttatcagcg gcaaggtgca gtcctccggc tttggggaaa
     1021 tgctgaccgt cctcggtcgt accattgtag gccttcgcag tacgatcatt accatcattg
     1081 ccgtcattgc aacggcaaag gttatgggat acagcggtat gacttcagaa atcgcgcaga
     1141 cggctgtcaa tgcgacggga tccttgtacc cggccattgc gcccttggtc gggtcgatcg
     1201 gtaccttcat caccggttcc gggacgtcga gcaacgtttt gtttggcaat cttcagactg
     1261 atgcagctgt cgccatcaat gctgataaag gttggttggc cgcggcgaat gcagccggaa
     1321 cttgtgccgg caagatgatt tcgccgcaga gcattgccat tgctgtcggg gcgatttccc
     1381 taaaggggaa ggataatgag attttacagg ctattgtgac cgtatatcct tattttaggg
     1441 ggagtcgttt attttggtca ggcctttatt ccctgaatca ctaggggaga ggcggacacg
     1501 atgtatgtcg tgtccgcttt ttttgttgtc aggaatcgat acactataat gttcttaata
     1561 caaactattc ctcaaacaaa cttagtactg aaattcacga accatgccta attatcataa
     1621 ttgaaaagag acagtacttt ttaatcatgc aaatttcagg acacttttta tatttatgat
     1681 attgattatt gggaacaaga tgggctccat attttgaagg aggacataaa agacgagagg
     1741 tcgtcgttgt aaagccattg taaattttta atgaggaatg cttacagaat agattcgagt
     1801 tttcctatca aaactattaa ttcttaaacg gaattaattt atagattatt cattccataa
     1861 aatgaacttt ctcaataata ttcgttgacg ttatcgattt taagtgatat cattctatta
     1921 agtaattgag atatggaaaa ttttcacaaa gaatgaacga tatcatgtcg aaccattcaa
     1981 tgtgcaggag gcaatcaggg gaatgagaaa agttaacatc attacggctg aacaagcagc
     2041 tcagctggta aaggataacg atacgatcac atcgatcggg tttgtcagca gcgcccatcc
     2101 ggaagcgttg accaaagcct tggaaaaacg gtttttggaa acgaatacac cgcagaatct
     2161 cacctatatt tacgcaggtt ctcaagggat tcgcgatggt cgtgctgccg aacacctggc
     2221 tcatacggga cttttaaaac gagccatcat cggtcactgg cagaccgtac cggctatcgg
     2281 gaaattggct gtcgataata aaatcgaagc ctataacttt tctcagggca ccctcgttca
     2341 ttggttccgt gctttggccg gccacaagct tggcgtcttc accgacatcg gtttggaaac
     2401 tttcttggat ccccgccagc tcggcggcaa actgaacgac gtaaccaagg aagacttggt
     2461 taaactgatt gaagttgaag gccatgaaca gctgttctat ccgaccttcc ccgtacaggt
     2521 cgccttcctt cgcggcacct atgccgatga atcgggtaat attacgatgg acgaagaaat
     2581 cgggcctttc gaaagtattt ccgtggctca ggccgttcac aactgcggcg gcaaggtcgt
     2641 cgttcaggtc aaagacgtcg tcgctcacgg cagtctcgat ccgcggatgg tcaagattcc
     2701 cggtatctat gtcgacgatg tcgtcatcgc cgctccggaa gatcatcagc agacctatga
     2761 ctgtcagtat gatccgtctt taagcggcga acatcgggct ccggaaggcg ctgccgagga
     2821 agcccttccc atgagtgcca agaaaatcat cggtcgccgc ggtgccttgg aactgaccga
     2881 aaatgccgtc gtcaacctcg gcgtcggtgc tccggaatac gttgcctccg ttgccggtga
     2941 agaagggatt gccgatacga ttacccttac cgtcgaaggc ggtgccattg gcggcgtacc
     3001 gcagggcggt gttcgcttcg gttcctcccg caatgccgat gccatcatcg accatacgta
     3061 tcagtttgac ttttacgatg gcggcggttt ggatatggct tatttgggac tggctcagtg
     3121 cgacagcacg ggcaacatca acgtcagcaa gttcggcact cacgttgccg gctgcggcgg
     3181 tttcccgaat atttctcagc agaccaaaaa tgttttcttc tgcggcacct ttacggcggg
     3241 cggcttgaaa gtagctgtgg aagacggtaa ggtcaagatc ttaaaagaag gcaaggccaa
     3301 gaaattcatc aaggccgtcg atcagattac cttcaacggt tcctatgccg cccgcaatgg
     3361 caaacgcgtt ctctacatta ccgaacgatg cgtctttgaa ttgaccaaag acgggctggc
     3421 cctcatcgaa gtcgcgccgg gcatcgatat cgaaaaagat atcttggctc acatggactt
     3481 caaaccgatt atcaataacc ccaaaaccat ggacgcccgc atcttccagg acggtcccat
     3541 gggactgaaa aaataaatct ctcgctgtaa aggagacctt actatgaaac caatgagact
     3601 ccatcacgta ggcattgtct tgccgacgtt agaaaaagcc cacgaattca tgcagaataa
     3661 cggccttgaa atcgactatg ccggttatgt cgacgcctat caggccgatc tgatcttcac
     3721 gaaattcggt gactatgcaa gcccgattga aatgatcatc ccgcactccg gggtccttac
     3781 caaattcaac ggcggccgcg gcggcatcgc gcacatcgcc tttgaagtcg acgacgtcga
     3841 aggcgtccgt aaagaaatgg aagccaagtg cccgggctgc atgctggaac agaaagccgt
     3901 acaggggacg gacgacatca tcgtcaactt ccgccgccct tcgaccaacc agggtatcct
     3961 cgtcgaatac gttcagacga cgg
//
LOCUS       DAWDXZ010000111         3955 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5521_length_3955_cov_10.242564, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000111 DAWDXZ010000000
VERSION     DAWDXZ010000111.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5521_length_3955_cov_10.242564"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          990 a          981 c         1017 g          967 t
ORIGIN      
        1 gttcagccgg aaagccgcaa gcgtctgatg gcggcccagt atctgcaagg ggttacgctg
       61 accgtcggag aaccgttata accggcatgc cgcatgcatg gccatacgtt ttatttagga
      121 ggattcatga acgtacgtga aattgccttt caaagcctgc tgaccatttg cagggatgaa
      181 gggtatagta atatcgtcgt ctcgaaggcg atacaggccc ataaattttc tgatcgggat
      241 cggagatttt atacggaact tgtttatggg accctgcgct gtcttaatta tttggactgg
      301 atcatttccc agcttagtac gcgcaaatta aataagctcg atccggtttg cctggccatc
      361 gtccgcttgg ggctctatca gatttttggc atggataagg tgcctgaatc ggcagcctgc
      421 aacgagtcgg tcaagatggc gacgaagttt ggcaataagg gcatggcaaa attcgtcaat
      481 gccatgctgc gaaacagcat tcgcaaccgc aattccttcg tcattccgga taaggaaaag
      541 gatatgacag cctacttggc cttgacgtat catcagcagg actggctcat tcgccgttgg
      601 ctgaaagatt acggcccgga ggaaacggaa cagctttgtc agtatttcga taccatcccg
      661 cctctgtgtc ttcgggccaa tacgacccgt accagtcggg atgagcttct taaagacctc
      721 accgaacaag ggattcaggc tcgtccgacg ccggtttcgc cggacggtat ctacatcgac
      781 ggtcacttgg ggatccacga actggagtgc cttaaagacg ggcgggccat cattcaagac
      841 gaaccgtctc agctcgtcgc ccatatcgtc gaccctcaac cccatgaact ggtcctcgat
      901 gtctgcgctg ctcccggcgg aaagacgacc catttagcgg ctatcggcgg tccgtcgtgt
      961 atcgtctacg gcggcgacat ccatgaacat aaattgaaat tgattgacta taatgcccaa
     1021 aaattaggcc ttaaaaacgt ccgcaccctc ttgcagaatg cctgcaccat cggtcagaag
     1081 tacggcggtc gggcagaccg cgtcttgatc gatgcgcctt gttccggtct cggagtcctg
     1141 cgccacaaga tcgaccttcg ctggcgcaag cagttgagcg atcttcgtac tttaccggcc
     1201 ctgcagcgtc gcattttgga aagtgcgtct cagtgcgtga agcccggcgg cgttttggta
     1261 tacagtacgt gtactctgaa tgatgacgaa aacagcggca tcgtcaacga atttctacag
     1321 aagcatccgg agtttcatgt tgaaaacgct gcggagaaac tggccgttcc gggacatgaa
     1381 ggccccttta tccagcttct tcctcagcgt gacggcatgg acgggttctt cattgctcgc
     1441 atgaagaagg agtcttgata tgatgaatct attcgatatg acaaaaaaag acctgcagga
     1501 cttcctcgtc gcccaaggga tgaaaaaatt ccgggccgat cagatttttc attatatata
     1561 taagcagtcg gttttttcct gggatgacat ggtgctcctt ccgcaaaagg atcgggacct
     1621 tttaaaagaa gtcctgccta ttcagatgcc cgacgtcgtg tcgcgccttg atgcaccgga
     1681 cggacggacc gttaaattgc ttttgcgcct taacgacggg cagaccgttg aaacggtgct
     1741 catgatgcac gattacggaa attcgatctg cgtttcgtcg caggtcggct gtgccgtcaa
     1801 ctgtgccttc tgtgcttcgg cgaaaaacgg ttttgtccgc aatctgtcgg ctggagagat
     1861 ggtggcgcag ttattggcgt tccgtcaaca cgtgacaccg gagctgcact ccatcgtcat
     1921 catggggacg ggagaaccgc tgctcaatta cgacaatgtc atcgccttca tgaagctcat
     1981 ccacgaaaag gataccctct acttaggcta tcgcaatatg accttgtcga cgtcgggcat
     2041 cgtgcctaag atgtaccaat tggcgaaaga agggatgccc attacgttgg ccgtatccct
     2101 ccacgctccc aatgatgctc ttcgtcgtcg tatcatgccg attaccgaca agtatcccct
     2161 cgatgccgtc ttgacggcgg cccatcatta ttttgaagag acaggccgga aggttaccta
     2221 tgaatatatt ctcatcaaaa atctgacctg cacccattct tgtgccaaag aattggcccg
     2281 ccttttcagt ggtaaaaata ttctcatcaa tgccattccc attaatgaca actatgacgt
     2341 cggtctctat cggcctgatg ataagtcaat cagtgaattc gttacctacc tccagcgcca
     2401 cggcgtgccg gtcacgctgc gccgggaaat gggcagcaag attcaggcgg cctgcggcca
     2461 gctgcgtata aaacgtgaaa atatttgaca ggtgacaggg aatatgatat gaattaatgt
     2521 gtacctataa cgaaacaccg tgagaggacg tgagaatttg ggcacatacg gtgaatccaa
     2581 aatcggcctg gttcggaaag taaatgaaga ttctttttat atcagccatg agcgaaacgt
     2641 gctggccgta gccgacggta tgggcggcta cgtcggcggc gaaatcgcca gtaagacggc
     2701 ggtagaagcc attgcttact attttaagaa ctttaattat gcggcaccgg tgcagctgga
     2761 aaaggcaatt cagtatgcca attccagtat catcagcaaa accttgatcg acccgtccct
     2821 aaagggaatg gggacgaccg tttccatggt gaccctggcc cggcatatgg ccttttgggg
     2881 ccacgtcgga gacagccgca tctatctata ccgggaagga gtcttttcac agatttctgt
     2941 cgatcatacg attgttcagg ttttactcga taaaggtaag attacagaag aagaagcgct
     3001 ggatcatccg cagcgccatg tactgaccag agcggtcggt gtcgactcca atctcatcgt
     3061 cgataccggg gcttttgaag tacagccgaa tgaccgtatt ctgatttgtt ccgacggctt
     3121 gacctctttc attggcaagc cggaactttg cgatgccatc ggcgactacg gccggacaga
     3181 acgggagatc atcaacgatc tctttgacca ggtctatgaa aatggggcca aggacaatgt
     3241 cacggcaatt ttaacaacca tataatcggg aacgactatg aaagtggtgg aatagttaaa
     3301 aatgattggt catatcttag acaatagata caagatattg gaaaaagtag gcacaggcgg
     3361 catggccagc gtatataagg cccaggatat tctcctggat cgtatcgtcg cagtcaaggt
     3421 gcttcatgca aaatacggcg acgaccacga ttttgtcgtt cgttttcggc aggaagcgca
     3481 agcggctgct aaattgtccc atcccaatat cgtcaatata tacgacgtcg gctacgatga
     3541 aaatgtccat tacatcgtca tggagtttgt acgcggcgag acgttgaaag agtatatcga
     3601 aaaacacggc catttgccga tcaatacgtc gatacagatt gctttcaata tcggcgaagc
     3661 ccttgaaaac gcacatgcta acggcatcgt ccactgcgat attaagcccc ataacattct
     3721 ggtgactgaa acggggcgta ttaaggtcgc agattttggc attgcccggg ctgtcaattc
     3781 cgtggaaaac agcgatcgca ccgttctcgg ttcggttcat tatttttcac cggaacaggc
     3841 cagcggcggc catattgatg agcggacgga tatctattcc ctcggcgtcg tcatgtatga
     3901 aatgatgacc ggcatcgtgc ctttcgaagg ggatacagct atcagcattg ccttg
//
LOCUS       DAWDXZ010000112         3929 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5556_length_3929_cov_8.315436, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000112 DAWDXZ010000000
VERSION     DAWDXZ010000112.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5556_length_3929_cov_8.315436"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2803..2827
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1104 a          818 c          944 g         1038 t
ORIGIN      
        1 ttgaaaactg ctgcagtacg gtccggatat cttccggaat ggctgccagg gtccagaaat
       61 acagaaagct acaatcagac tatcaaaaac agtttggtca caagccctct gctgacgaga
      121 tacgggcact gatgggaata agccggtcag aatatgacac catgcagcag gccgtcagaa
      181 tgggcaccat agcgagcctg tcagccccta caggtgagga cgatacaacc actatcggcg
      241 acaccatcgg caacgagggc atagaggatg ctatcattga ccgtatcgac caggaggaca
      301 tgaaacggga catacatgca gccgttgaca agctgccgga ccgtgaacgg gaagtaatca
      361 ccttgcgata tttcgagggg cttactatgg aacaggtcgg agagcttgag agccggagca
      421 tggagagcgt cagacaatgc caagctaaag ctctgagaca cttaagaagc ccaagttaca
      481 gacggccatt agaatgttat gcattgtcag acaggcagct ttctgcagca atgcacagca
      541 ctgtcagcgg attcaatagg acatggacca gcgccacaga aaaagcggct tttatcggcc
      601 ttgctgccga tcgtgtgcga tactaatttg atactaaaaa agtatataga ctgtaaaaac
      661 tgtctatttt gagcaataaa agtagtgcaa aaatgctaaa atatagtgta taatgttgtg
      721 gaaaacgcaa agccaatacg ttataataca acgaaaaaga ccagaaaaca ttgaaaaacc
      781 ttgctttctg gcctttttat aattccatat gatactattt tgatactaat ttgttgagtt
      841 tgtcggctac ttcctggtgc cggttcgggt acagatgccc gtaaaccttg ttgatcatct
      901 ccggggtatc gccgatacgt tcagcgatta gaaacggcgt gaagcccata tcaattagca
      961 tagatacgtg actatgtcgg agctcatgta tccggatgcg tttgatgccg gtgctctcac
     1021 agccgttttt tattgctgtc tgaagctttg attgtgtgac gggctgaaat atccggtcat
     1081 ttggctggat gtcgtaaaaa tgggtcagat attctttcaa gacatccacc aggaacggtg
     1141 gcagcgtaac agatcggacg ccccgcactg attttggcgg tcctacatgg tcggcgctgc
     1201 catatcgctg ataggtttta tttaccagca gagcgtcgtt atcctgatca aagtccgcaa
     1261 gggtgagcgc ttgcagttct ccaatccgta gcccggtaaa gaacagcact tgaaaagccg
     1321 tgtgtaatgc gggatctgtc actgtattca gaaacttccg gaactcttcc aaggtccaga
     1381 aattcaagcg cctaacaccg gctttgccca tagccccggc aagcttgcac gggttctgtt
     1441 tgagaccgta atatttcacg gcataattta atatctgagc aaggatggaa tgcaccagaa
     1501 agcaatatga ctcgctgtat tcctggtcaa tgatagcctg ctgccatttc cggacggctg
     1561 caggcgtgat cttgttgatg gccgtatcct tccagtatgg cagtatatgc ttctctatcg
     1621 tctgcttgcg cattctatag cttgtgaacc tcacacggct tttataatcc ttcagaaaca
     1681 attccaccag ggcggaaaac ttcatgtctg attcgcccgc ctgctgcgtg agaaagtcat
     1741 tttcccactc tttagcatct ctttggagct tgaagccccg tttcatcttc tggcgcttct
     1801 ggccagtcca atccgtataa tataacttca cataccatgt attatgcttt tcatctttat
     1861 ataccggcat tgtatccctt ctttcattcc gtggtgctgg ggagcggctt tgaaatgatt
     1921 gaaagggtgt gcatagtatt cctaccctta cgccctggga aggcagaacg attcgttcac
     1981 cctctgcaag gtgccgaaat ttcgacgaaa cggggaacta attcggtttc tatttttttt
     2041 tagaaagcaa gtttacaagt gtacttttgg atgccctgtt ttagggtagc cctgcaatgc
     2101 gatgttgtaa attgcgacat catggggtgt aacgttttgt gactccccat aatagggggg
     2161 gcatagtgtt ttaagatagc cctatataca atatctgacc gaacggtttg ttatggcagc
     2221 tcggcggtgt tttggcgttg agtctaaccg gtttcgcttt ccatttttag aaagcaagtt
     2281 tacaagtgta ctttaagtac ggtggttatt gtcaactttg acgataacta ttcgtcctga
     2341 tcggaagggg ccgaagattc gttttttggc tgtgtgttgg cacgatattc aggtattttt
     2401 gtaagcattt ccacctgttc aatagctttt tgctggccaa cactgttcaa agtatcaaag
     2461 tgatacaaaa gttcaatttg cttttcttcc gcaatgcctt cattgatcat atggctttca
     2521 actactgcag aaatttttcg cacaaagtct gaattataaa cggatggttc tgaacttata
     2581 tatccatttc tcaggtctga attgcggtgt acttcaaaaa ctctcaaacg tccatgtgcg
     2641 ttctgataat cttcaggctg aatgtcaaac ccgagcaact cactttctgt aacgtctaat
     2701 gcttcacaaa ttttttttat aatatcaaag cgcgccattc ttccatctcg tttagttata
     2761 gaatatagag tattaacaga tattcccgtt tctttggata agnnnnnnnn nnnnnnnnnn
     2821 nnnnnnncta attaggaagg aggcaaacat gaaaattaac agaaaaaaat tggatttagc
     2881 caaggcgaga gcctgcatgg gttcgaaaga aattgaagcc ggtggatttc ccaaaggcac
     2941 ctattgcgga atcctgcaag gccgcgaagt taaaccggaa acagctggca gacttgcccg
     3001 cgtcctgggc tgtgacgtaa cagacattat tgattaaccc gctggggagc ggctttgaaa
     3061 tggatgaaag gagaaagcat ggagtttaga acattcaatc aaaatgagga tgaaccggcc
     3121 aacggctgga ccggcgaaac ggtacggcat gcattgcagc agggaaagaa gcttgtcgtt
     3181 gatcagggcg gtgacatctg ggaacttgac caggaaggcc gccaaaaaga atactgcggg
     3241 aagatattga gaaaggagaa gcaaatgagc gaatacatga acgccaaaga actggccgag
     3301 atggtcagcg tgtcagaaag taaagcatac tgctttatcc gccagatgaa tgaggaattg
     3361 aaagccaagg gatattttat cgtccgcggc aaagtaccgc gtaaatacgc ccgtgagcgc
     3421 ttcggggtga gtgaataatg gcatcaagac gaatgtttag caggagaata acagattcta
     3481 gccggttcgt aagaatgccg ttgtctgcgc aggccttgta ttttcatctg gggcagcatg
     3541 cggacgatga cggagtagtt gaatcctatc ctgttatgaa gatgatagga gcaaacgagg
     3601 atgacttaag aatgcttata ttaaaagaat tcgtcattcc tctgaataat gattacgtgt
     3661 catatctgcc agattggatg gagcacaata aaatcagggc agaccgtaaa gaagacagtt
     3721 tgtatattga tttgctttta caggttgtac ctgatgccga aataaggcag gctaaaccgt
     3781 cgtattattc taatcaaaaa agcatttgtc tgacaaatga cggacaaatg acagacaaac
     3841 gacgggcaaa tgtccgcata ggtaaggata gtataggtaa ggatagtaaa gagataaaag
     3901 atgtaacgcc ttcgaaaaaa tcgaaggct
//
LOCUS       DAWDXZ010000113         3901 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5595_length_3901_cov_7.721269, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000113 DAWDXZ010000000
VERSION     DAWDXZ010000113.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5595_length_3901_cov_7.721269"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1016 a          990 c          900 g          995 t
ORIGIN      
        1 gctcaaaatc gacgtcgtgc aggaggccga cgagccccca aaagtcaacg tcatcgccgt
       61 accctaattc ttgcgcatac cagcgaatga ccccttcaac ggtcagggca tgcatgatat
      121 gaaaaggttc tttattatat ttttgcagta aagccaatgc ctgcgagcgt tccatagcgc
      181 ctcgcccact cttaatcatt gatttatctc acattatacc ataaccccgc ataaaaacaa
      241 ggtcgttcat catgtttacg cttataattt acattttata tcctgttttt cagtcgattc
      301 atgcgaaaat cttcgacaat ttgaatgaag ggacggtagt gaatggggtt ctctgtaaag
      361 ccaaagagat gggacagaca atggcaatcg gtgcagccaa atccgtcgat acgccaatcg
      421 gaaatcacct cgacagctcc cgccaattca tgttctaaat cctgggacgt gcgaatggga
      481 atggcagcaa tgacatcagc ttcctgccgc aggtagtcga tgtgccaatg gaatttttta
      541 cgcttacggc ggtgacgagc cagtcgggcc gctaaattct tcttagccga tccggtatag
      601 acgtagtagc cccttggaaa gtgcattatc cctttcgatc caatagcaat atcggtatca
      661 tctgcgattc ggatgacgaa aagatagtcg ccgctgtcat gattttcggc cgttaacacg
      721 gcttccggat aggttaggag tcttaccggt tccggccttg agaacgagga gtcccagcga
      781 agggccaagg ccttccaatc gaggagaggt gccgcttcat aaaaggcttc ggcaaaattg
      841 ggatccgtat ggtaatcggg aaggaaccac cgtgcccggt cccaatgaac gaggaataag
      901 acgcccgtac ggattccctt ctcgctcata gacgccaatt ctaaaatatg ctttctccct
      961 cgttcggtga cggcatcggg gaacatcgcc ccatcgtcac tgaataaggt acaggatttc
     1021 acttcgacga aaaatcgttc ttcgcccttt cctaaaagga gatcgaagcg gctgtcgcca
     1081 acagtcactt cacggcgaac gaggttccat tcttcccaac ccggaatgag atggttttcg
     1141 acaagccagg ctgccgtatc gttattatat tgtgtatcga gtaaaatcgg aacgccgtcg
     1201 cgttcgatgc cgacgacgtc atacggcgtc ttgcgcttgg gattgtctgc cggccgcaca
     1261 taaacaatcg tccccttaaa aagaagttcc cacatccttc cgggatttgg catatggcag
     1321 gtaatcacct gtccgtccaa gtcaacgtgg gcaataaatc gattgggtcg atctatgaat
     1381 ttacccttct gtatctgttt catttccata aaatacctca catatgagaa aaagaggctg
     1441 ccattgacag cctcttttta ttatatacag ttataaatta atcttccagc cattcgccgg
     1501 taaaagtaac ggcagccgga cctgtcatat agacgttacc gtctttttca tcatattcaa
     1561 tgtgaagggt tcccaagtac agctgaacgt cgacgctgcg tccggtaaaa ccattcatga
     1621 aagaaccgac ggcgcaggcg caggaaccgg ttccgcaagc caaggtagcg ccggcaccgc
     1681 gttcccaggt atggaccttg atcgtatggt cgtcgatgac ctggacaaag ttcatattgg
     1741 tcttacgcgg gaaggcctta tgggtttcaa atttaggacc taattcatga agggggacgg
     1801 catcgacatc ttttacatag accatcgtat ggggtacgcc catgagcagg ctggtaatct
     1861 tatacgtctt gccatctact tcaaggggtt cgtcgacgac cgggccttca gggccttcca
     1921 taggaatctg agaacgattg gtaaagggtt tccccatatt gatggtaact aaacttactt
     1981 ttccgtcatc gccgagaatg accttgggct tcatgatacc ggccaaggtt tcaacggtaa
     2041 attctttttt gtcaatgatc ttatggtcat agacgtattt tgcaaagcag cggataccgt
     2101 tgccgcacat ttcagcttca cttccatcgg cattgatgat gcgcatgcgg acgtcggctt
     2161 tatcactggg aacgatgacg atgataccgt cagcgcctac gccggtatga ggcgcacaca
     2221 gcttgatcgc taactgggag aaatccttgt cggctccctg cttatcttca aaaaagacga
     2281 aatcattgcc taaaccgtgc attttgacaa atttcatatc ttgtaccctc cattaaaaat
     2341 tatatactca taatgattga tcattaactg tcttgaatcc ttacagcatc tattatacaa
     2401 taactgaaag tgaaaagtat atgaaaataa tgttatctat attgcaaata atgctatttc
     2461 aagataactg aacttccttt ttaagaaaag tcaatcaaag agagaaggaa agactgacat
     2521 ggcacaaaaa acaggctact tatcgggtaa tctaaaactg ttccacctag tcgataaaag
     2581 gcagtggacc tgtccctacc attatcatga ctttgacaag atcaccctct ttttccaggg
     2641 acatgtatct tacgacgtcg agggaacggc ctacgattta aaaccctttg acatcgtcgt
     2701 tattccagcc ggaaaaatcc atcgtccgag cgttaccggc gacacgcctt acgagcgcat
     2761 catcgcctat atttctccgg cttaccttaa gacatacgaa cagcgcggtt gccctgtggg
     2821 ccaactattt tccgactccg cctcgcccat cctccgccag ccccaggaag cggacagcct
     2881 gtacaacgta tcctgccgtc tgaggcgagc ctggtcttat acggacccgg aagggaatat
     2941 cctgcaggag gccctgtttt cggaattcct catccatctt tggatggcta tgaaaacgca
     3001 gaaaatcggc tttgtaaaga aaaaacaaca gcaccaaaaa atcgcccata tcgtcgacta
     3061 cctcgaaaat aatcttaccg ctgacctgtc gattccggcc ctggccaaga gattctatat
     3121 gagtccggac tacttgatgc acctcttcaa aagtgagacc ggcatgaccg ccggcagtta
     3181 cattacggcc agaagactgc aaaaagcccg ccgcctcatg caggagggac gggctttgac
     3241 agagctttgt tacgaatgtg gatttaccaa ttattcgacc ttttatcgag cttggaaaaa
     3301 acgctaccat acgtcaccga aagagggcct catcgaaaag gacgatctct tcgatgaatg
     3361 aggatcagcg attgcggttg tagttgatca gactgccggc tttaacgatt ttcttttcat
     3421 cgtccgtcat gggagcgata tagagggaca gttccttcat gcccttggtg acgacatagg
     3481 ctttaatatc cttcatgtcg ccgtcgaggg cctttttgac gtcgggaacg taaatatagt
     3541 cgccgatttc aaaatccggt tcgccttcca tttggaaggg cagcatgccc cagttgatga
     3601 cgttggagcg gtagcgcttc gtcgcatact cgctgcagat attggccagg ccgccgagga
     3661 cgcgctggca gctggctgcc tgttcacggg ccgaaccgtc gccaggtttt acacagtaaa
     3721 tcatactgcc gatttcaacg tctttcatgg aaacgccgcc aaaacgctgt ttcaaggctt
     3781 caaagacagc ttttaaatcc ggatcgacac cggccgggca gtctcctgct aatcgagcct
     3841 gttcgacttt gtttacggct ttagcccgtc cgacgtattc cggatcacgg cgcgacaggg
     3901 t
//
LOCUS       DAWDXZ010000114         3846 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5660_length_3846_cov_6.192561, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000114 DAWDXZ010000000
VERSION     DAWDXZ010000114.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5660_length_3846_cov_6.192561"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    768..777
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1852..1861
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          971 a          934 c         1072 g          849 t
ORIGIN      
        1 tatggccggc atggtcaatg acgaaggccg gatctatgta tctgaaaacg agccccttac
       61 gacgcagctc attgctaaga gcctgcaccg ccgccagccg tttattgaaa acggactgcg
      121 ggcactcgag caagtcgacc tcatcgaacg cgacgaaaag ggcatgatcc gtatcgtcat
      181 ttgggatgag ctgcaaagct ttcatcgtga cgagaggcga agggagcaga cctctgaacg
      241 ggttcgccgc tatcgggaga ggcagcgcct gactcaagag gaagaagctc aacaagagag
      301 ttttcaagaa gaaagtctca tcgctgaacc ttccggtgaa gaccattcgg atactctgga
      361 cgaagaaccg gttgccctgc ccatcgatga gctcgtcgaa gaagctgtcg tagccgaaaa
      421 ggcgggtgac atgccgctgg gagaaattcc ttctacggat gaaggggaag tgtctcaaac
      481 agaaccggaa cggcttacag gcgaagcggt gacgacgccg aaggccgtga cgggctacgc
      541 ctcggtccgg acgtatcaga acctatttgg gcccatgcag ggcgattttg ctcatcgatt
      601 gatcaccttg gaaaacatgt ggggcagtga gcccacttgt acggccattg agatggctca
      661 cgaaaacgag gtcaataata taaactatat tgccgctatt ctaaaaaaca gcaacggacg
      721 acccatgcgc ggcaaacgaa aggaagataa gtaccatggc tatccgannn nnnnnnntct
      781 aagcgaatca tgaaaggcgg tgctttttcg gatcatgacg gacttttcgc atcggacgtc
      841 gtttttgaag gctgaaaatg atcgtgattc ccatagatat cgaaagccca agagatattt
      901 aatgccccgg cagggaccca tcgtcatcga aatggaaaac ttttttcaaa aaaacgcttg
      961 acaggtagtt actaccggtt gatatccttg tttcaagaag agcgatacaa gctacttctc
     1021 atacaaaacg tataaatctg aaaggttggt tatcgtgaac caaagtatca agaaaaaaat
     1081 atggagtcta tttttgatgg gaggtttgat gatgatgagt atgaatggaa atacggcagc
     1141 cgccgcttcg ccgaatggga atccctttgg cctcgtctac ggcaatgcca ttgaaaagaa
     1201 tgaaccgggc aaggtgaata tccgccctgt cgaatatgaa gtggatggta tcaaagtgtc
     1261 tgccaacctc tatttgccgg ccgattatga cgaaaagagc gataagaagt atgccgccat
     1321 taccgtggcg catcccaacg gcgggacgaa ggaacaggta gccggcctgt acgcccagcg
     1381 cttggctgaa ctcggctata tcacggtcgc tgccgatgct cgctatcagg gagccagcgg
     1441 cgggacaccg cgccttcgcg attacccgtc gaaccgtatc gaagacgtga gcggcatggt
     1501 cgattacctt tctacgctga ataaagtcga tcgagctcgc attgccgccc tcggtatctg
     1561 cggcggcggt ggttatacgc tggcagcagc ccagacggat aagcgcatca aggctgtagc
     1621 caccttgtcc atgtttaact ccggccgggt acgccgcaat ggcttccacg atgccgatat
     1681 ggcagggatt ccggctcgcc ttcaaaaagc ggccgatgcc cgggaaaaag aactttccgg
     1741 cgagatcgtc tatgaagggt tcctgcctcc taacgctacc gatgacgatc ttcgggctaa
     1801 gatggcagaa cttccggaaa ataccctcta ccatgacggt atcgaatact annnnnnnnn
     1861 ntgacccatc gccatcccaa tgcgacggga agctacacga cggaatcgct catgaagctc
     1921 atggcctttg acgtcgaaga tcggatggat ctcatcgatc agcctctgtt gatgatggcc
     1981 ggcgataagg ccgacaccct ctacatgacg gaagatgcct ttgccaaagc cagcggtacg
     2041 aacgataagg aattgttcct catcaaaggc gccagccata ttcggaccta ttgggttccg
     2101 gaatacgtcg atgaagctgt caataaactg gctgatttct acgggaagcg tctgtaagcg
     2161 taagcccata cgttgtaacc agttcaactg ggtatgaaaa gccctgccgg tatcggttta
     2221 gggcttttcg gcctaggggg attacgcctt gtcatggcgt ttgacggttt attttttgga
     2281 tatcccttga tacgggcagg gcctaaaaaa agtgcgaatg accttctttg agcgccgtat
     2341 ccggggatac catgagaaag gagacgattt tcgaagatga ttaagaggac aaccggggcc
     2401 ttttcgttgg attcaaccat ccgcgatgtc atccaggatg aggcattttc cggcttcggc
     2461 cgcctgctgt ttcccgtaga tctggcgctg ccgccgcaga tgacgttgaa ggatttggga
     2521 aaagcctttc tttggtacag ctatataaag ccggagcgga cggtgagcct cgtcaatgag
     2581 ctgaaagaaa gggtccttgc cggggagacg gttttttacg atatttacag tccggcggaa
     2641 aaggctgccg atccgaaaaa gaaggatacg gggttatttt tcttccgcgg caagcctcag
     2701 ggcaagactg ccattctcag cgccggcggc gggtttgtct atgtcggtgc catgcatgac
     2761 agttttcctc atgcccagga gcttgccaag gacggttaca acgcctttgc cctcatctat
     2821 cgcgtcggtg cccaaacggc ctgtgaagat ttgtcccggg ccattgcctt tatccatgac
     2881 catgcaagag aactaaatat cgatgtcagc gattattccc tctggggcgg ttcggcaggc
     2941 gctcggatgg cggcctgggt cggtacgtat gggacgggag cctttggcga aagaccatac
     3001 cctcagccgg cggcggtcat catggagtat accggattgt ccgatgtcac cggaagggag
     3061 ccgcctacgt ataactgcgt cgggacggcc gacggcattg cctggtatgg caccatgaaa
     3121 cgacggatcg aaggcattcg ggcccatgga accgatgccg acattgaaat tttcccgggg
     3181 ctgccccatg gctttggcct cggagaaggg tccgttgccg aaggatggat ccatcgagcc
     3241 gtttcttttt ggcagcggca gatgaaagat tgaaagatta ttttatgatt tggatgacag
     3301 cctttggctg tcagaaagga agcatcatga gtattttatt tatcaacgga agccccaata
     3361 aaaatggaaa tacggccaag atggctgcgg aactccttaa aaatcagacc tatgcaacgc
     3421 tgaacctcgt cgattaccgg atcaatgcct atggacagga cctgcccgga gaccagttgg
     3481 acaccgttct cgaaaagatt gaagcggccg atgtcctcgt cgtcggatcg ccggtgtact
     3541 ggcataatat ctgcggctcc gtacgaaatg tactggaccg tttttacggg aaggtttccc
     3601 aagggagctt atccgggaaa aaactcttct tcatcttcca gggagctgcg ccggaaacgt
     3661 ggatgctcga agcgggcgag ttcacgatga aacgctttgc cgccttatac ggcctgactt
     3721 atgtgggcat ggccaagaat gcgaaagaag cgggggacct ggccgcttcc ttataagtta
     3781 gaaagaatcc tgtcggacag gcgtgcctaa gaaggcggga gaaaagtgac tatttaaaaa
     3841 aagagg
//
LOCUS       DAWDXZ010000115         3806 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5702_length_3806_cov_8.506532, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000115 DAWDXZ010000000
VERSION     DAWDXZ010000115.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5702_length_3806_cov_8.506532"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          989 a          794 c          951 g         1072 t
ORIGIN      
        1 ggttcatcga ttgggatgta agatcacagg tgccgccggt ttattataaa ttgaagtggt
       61 ccgaagccga tcaatggata ggctatgact acgtcggaac ccagcggccg tgagatgtcc
      121 ggcggcaaga cgccgttacc gaacgaggga gcttttaggc ctcgtccact ttttgaaagt
      181 gaggggaaac tttatgataa aaaatatatt ccgggcctgc gtattttcgg cggccttact
      241 gatcgggagc atggcctcag cctatcatgc cgacctgacg tcggccgtca atctcggcat
      301 ggtcaagtct tacagccgtt cggccattaa tgttattgat gacgacggcg ctgtctatac
      361 cttcgtctat gacaaccgcc ccgactcgga ttggggcttt tggaagcagg aagacgacga
      421 tcattggcac caatgggcct ttggcgtaca gcaaccgtct catctggaag agttggccag
      481 ctgcggtttt tatgccctaa aagatttagg aaaattatag gccttttaca agggcttgaa
      541 aagcaaggaa ggaaatgata atcgatgaat cagatggaat gggatcgcat gatggctcaa
      601 aaactatata atccttttaa cgtcggtgac gggagtttcg agcgcgtcca tacagcccag
      661 cgccgtttca atgcctccga tgacttgaat gataaggaag cgttcgaagc gctgaaaaag
      721 tgttttgccg aggctcctga cgacatggtc ttgttggctc cggtctactt cgaccacggt
      781 gatcgaatcc gctttggccg ccatttcttt gccaatacgg gtctcaccat cttggatgaa
      841 catgacgtca cctttggcga caacgtcttt ttggcgcctc atgtcagtat ctatacggca
      901 ggccatccca tcgatgccga agtgcgcaat accaacctcg aatatgccaa gcctgtcacc
      961 atcggctctg acgtctggat cggcggcaac gtcgtcatca acccaggcgt caccatcggc
     1021 gacgacgtcg tcatcggctc gggctccgtc gtaactcgcg acattcccag tcacgtcatc
     1081 gctgccggaa acccttgtcg cgtcattcgg cccattaccg agaaagatca cgacgattgg
     1141 cataaacagt gggacgaata caaaaaagca gccgcagcat atcttttgcg ataacctcgt
     1201 aaagactggg aaggattgaa aaggcatttt taaataggaa tcctatgacc atacaaacat
     1261 ctcttgagtc ataaaacttg gacggttaca ctaaatgggg cgtgtcctgc ggagagagta
     1321 acgaaattat ttttaggata aggctaaatt gtcgagactc tccatttcgt gtcgctgcac
     1381 taagagtgat gactcgggag aaattctatt tactaaaaaa ggtgtgatac tatgaaaatg
     1441 aaacgattca ttcccctcgt atggatcgtc ggtctgatga tgacggtcgg tgttgccttt
     1501 gccagtcata cggcaggact gcagccgact caagggacca gggtcttttc tgagaagccc
     1561 cagaaagaaa gtgtgccggc cctctccaat aaggtcattg aaaacgtcat tgctgatgaa
     1621 ctccagatga cggcaattca gcaaagggag acttcctatt actatcatta tgttgacctc
     1681 aatgatgatg gagttaccga cgccctagtc cttgccgtag gctcctatac ctccggaagc
     1741 ggcggcaaca ccatgttttg gctctcgccg gacaaaaggg gaaagtggca gattcagcaa
     1801 aaatggaccc tcgtccatac acctgttatg ctgaccggaa atgacgttaa tggctataaa
     1861 ggcatcgtcg ttcgacgtgc cggcggcgga gctactccgg catggattct cctgacctat
     1921 caaaacggcc aatataccac cgtcaatgac ggtatcattc tgccctttgc cacgaaacaa
     1981 taagaaggga cgtttccgct atgggacgat ttgattgaga tttcgttaat ttgattcgat
     2041 gtgatagaat aaaggtatca tgatggcgaa tgaggtgagg atatggccta taacttgccg
     2101 gatgctgttt tgcaggatat ttctcggttt gctgagaagc acgggattcg tcgcgttctc
     2161 ttattcggtt cccgtgccag ggggacgcag acggagcgca gtgatattga tctggccgtt
     2221 tgtggcgggg attttgacgg cttttactgg gatattaaag agaatgtaca ctctcttttg
     2281 atgattgatg ttgtggattt ggacgccggt gtttctgaag ggctgaaacg ggaattggaa
     2341 agggacgggg ttacgctgta tgaaaaagtt ggataatttt tcaaattgtc ttcgtatcct
     2401 gaagaatgcc gatttcatgc gaggtcataa tgatgaaatc tatcggacgg gtattgtcgg
     2461 tcaattcaat ttgacttttg aattggcttg gaaggccttg caacaggtgt tgagagtaca
     2521 cggtgtcttt gaagctgaaa ccggttctcc cagagaaatc ttgcagcttg cctataaaat
     2581 cggcttttta gaagatgatg cgacatggct gctcatgctg aaacaacgga atcgttcggt
     2641 atatatctac aatgaagaag aaatcaattc cttgttagtt ctcattcgag atgagtttgt
     2701 tccagctttt gctttcttgg agaagacctt acaagaaaaa atggctggca ttgaagacga
     2761 tggcgattga ttttaattgg ctcccatgga attacccctg tccgcattgc ttgttaaact
     2821 gtattgtact ggccattaca attttttgcc ttaaagctct caatttctga tcgtacctgc
     2881 ttttagacgc gaaagaaccc tggatttatg cttttacatg aacccagggt tctttgtatg
     2941 tgctttagct aaaatcgatg ttgaagtctt gaaaatccct tattctcaag attttttgtt
     3001 ttatgaaata gatgttttta aaatgcaatt taatctaata gcagagataa agcgaatatc
     3061 agtcagtcgt tattctatcc tatactcttc aactgtttct atcaaggtcg ccttgaattc
     3121 ggcgatgagg tttcgaatgc ttcggtcggt gaagcgattc atcagaagat atccctgacg
     3181 taatggcggt gggtcaagca ggcggcaaat ctgcaggggg atttcttgac gcagggcgta
     3241 ggcgttactt tcctgaagta tagcccaata tttacctgat atcagtgttg ctattgctcg
     3301 gcataacgac gtaatacttc tgattaagca aaaactcaca agttacccct tggactttat
     3361 gggctgcaga gactagtgct aaatccaatt ttttgttgga aatcatattg tagacgtcag
     3421 atgtttgata ggtataaatc gaaaaattag cagaagtacg agataatagt ttttcgcatg
     3481 tcctatgtac aagcgggatt tggtagctgg caattgatga aatgagtaat gtatgagcaa
     3541 tcatatcatt gtcattttgc atctcttgat atgcatcgta gagttttcgg gcttggggaa
     3601 gaaattgttg gcctctttct gtcagtgtaa tttctttgta ccctttgtcg cggacgataa
     3661 gggagtatcc taagatattt tcgagttggc ggatacggtt ggaaatagtt ggttgggttt
     3721 tatatagaat ttgcgaagcc tgggttagat tgcgaacttc actgacaacg atgaaggttt
     3781 caattaacgg atagtccata atcaga
//
LOCUS       DAWDXZ010000116         3798 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5713_length_3798_cov_9.411168, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000116 DAWDXZ010000000
VERSION     DAWDXZ010000116.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5713_length_3798_cov_9.411168"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    995..1004
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2488..2542
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          844 a         1026 c          825 g         1038 t
ORIGIN      
        1 gtcatgttgt ccgtgttgct gtaagcggcc atatcatgcg gattgcacac cctcgacgcc
       61 tcaaataact gtatcttggt tgagtagata ctgcccatcg tcggcttgcg atacttaata
      121 gccgcccagg cgtcaccatc cactaaatac gacaagtacg cgatgtcttg catatcatag
      181 aacgaatgtt ttctgtataa gtcgcattgc accgactccg cccagagcgc gaactcctgc
      241 tgtgtacgat gctgccacgc cttagcatcg tcggctgtca tccccagtag cttatagtca
      301 atcttaggga cgactctaag ccctgcaccg atgacattag cccggttagt attaacagca
      361 ctcgcgccga tcggcgagtt gatgtacata tccgacgagc gattgcgcaa tgtgctaagg
      421 ttagcatcga tatcagcccg cggcgatgac ttcataggat tgtatccgcg gagcgtcgac
      481 cgggaccgac tagcaccgcc ctcgctgtat ccggtattgg taattttagc gtccaggcgg
      541 ctcgtcggat gccgtacctt acgttttttc ttactcatga cgacctccta atcgcgtagt
      601 actacctgtt tggaccggga cgtagtccga ctggctccgt cgatacttgc accagcagcc
      661 aataatttac tgatctcact tcggatttcg gccaaatcgg cgcgcgtcaa ggtgcgatta
      721 ccgattttat aggactggcc gccggttaag atagccgttt ccgcggccat atactgtttc
      781 agccgcatat ttaacacatc gctcattcta tacctctctt aatcacgcca aatttagctt
      841 tctttcgccg aactggctta ctctttttag taattggctc gcctttgttc tgagccagcc
      901 agcgttcaaa tttagttttt acggatataa aacacgccaa gttatatact ttcaggtcaa
      961 taggttcatt tcgatggtct ttcgcaacgt tggtnnnnnn nnnnatacga tacgcccgtt
     1021 gcgtttttct ggtaattttt cttctgccgt gaggcccttg aaatagattt catcgtatcc
     1081 gcgattccat agtatttcac tgatagcatc tttttcgggc ttatcaatcg gaaaatgcat
     1141 atatttagga ccgggcacct cgatagccaa gcgatccatg atatactgct tagcgctgtc
     1201 cgtgccgata aatattaacg gtatcgtacg ccctggcaga accgtttttt ttgatacctt
     1261 gtgtataata ggtacgccca tcgtcgacga cccacggatg gctagccgct gacgagcaaa
     1321 ccgcgccagg caatacctat ataccgattc cccgtaatgc ccgccggcat cgataaatgt
     1381 cctggttacc gtcaaacctg taccatccgc ataatgatat gtgcggtcca gttggtcatc
     1441 aagctgttgc catacggcgg cggtatcagg tacgcctaag atgatgtcct ttttgatgcc
     1501 ccagctttct tcattttctc cccatcctac aatctcatat tccagtcgat tgtcttggac
     1561 gtcgaccgcc gctgtcagca ttagcacgcc atcaggcagc tccgcattgt acggctctcg
     1621 ccgtgccatt agcttatcga tatcgtcata gctacggaca tgctcatacg gctcgccaaa
     1681 gcgcgtattg acgactacct tttcgcgctc cgggtctcct tgagcatcta cccattcctg
     1741 catgacgtcg gtccacgata cccacggcga cgcccagcag ttcacaaaaa aactgcgtac
     1801 gtggttacgt atcgcagtag gattttttgc aacgtacttc tgcggctgac gtcgcatttc
     1861 attttcggaa aatgtgaagc cacacgcagg gcatcgccaa gacacggact tgacatgtat
     1921 atgttctttt tttcggccat ccttgaccgt ctccttatca gtggtcatat ctcgatgtgt
     1981 caacagatga tactcgccgc agttcggaca ttgatgctgc cactcctcct gcgtaccatc
     2041 cagatactcg acctcgatac gggattctcc ggcattcgtg ggcgttgaaa ataagcccat
     2101 ggtccggtcc cagaacgtcg tcatacgttt tgcggccagg tctaccgggt cgccttctgt
     2161 gccggcgctg tccgggaatc tgtcgacttc gtcggccagt aatatcttaa tcggtcgaga
     2221 tgccagccca gcaggactat tcgcgccgcc catgataagg cggccaccgg gaaataactt
     2281 actcaagatt gtattaccgg actcgcggct cttaacatca cggaaaatgt ccctcaaagc
     2341 cttagtatca cgtatcatcg gcgcgatacg ggacttggag taatcctgcg ccatgtccac
     2401 cgtcggctgg atcatgagta ttggcgccgg tgctaagtgc gcaaaacgac caatgacgtt
     2461 attcatgatg tcggattttc cgacctgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nncgccgcga tacgttggaa gagcttaatt gtcttctttt
     2581 tcatcgctgt cacccctttc cgcgaacatg tccgggcgat agtcagataa ttccgtcaat
     2641 cgcgattgga tttcatccgt caacgcctgc atgatagtcg attcatcctg tcctgacagg
     2701 accggggcca atttagccgg gatgcccagc aactgcgacc gtagattagt cagcatatct
     2761 gtcatgatga tttcgacgtc cttaccttca tggacttcgt tattgcgttt agccagttct
     2821 aactctgcca gtcgacgttt agccgcttca tgcatggctt tttcgcggcc gtagtcaacg
     2881 tcctccctag actcgtactt atagctgtag taagcctcta tgttagacgg caaatcatac
     2941 tgattttcct cgttccgggt caatacaccg gaccctgcca gctggtttac ccgcctcgag
     3001 gtaatccgta atagttcggc caggtcttgc gtagagccca ttattacttt acgtgtccgc
     3061 ctagccaata caatcacctc aaaatgtaca acagagaagg aaatgggcca aaaaatcgag
     3121 aaaactagcc ctttttcggg gtgcgaaagc gccgcggcag aatttttctc gtagaaggac
     3181 ccgtgaaaaa attgcaacaa aaaagagaca gccaaatgac tgcctctcca tatgatgttc
     3241 atactaccat tatacgtact atgatagtgc acttcaatgc actctttact tcagcggtgc
     3301 aataacatcg tgatacagct tgtcgaatgt acgcaacgcc tgaccatgga gattgaatac
     3361 accgcgcgaa ctgtagttca ggtccatcgc aatgtcttcc catctccggc aacggatata
     3421 ccgtgcgtac agtactgccc gttcgcgctg atctggaagt cccataataa gctcctttgc
     3481 ctgtattctc atgccaatca atacattcca ttcctgattt actctttcgg cgtatttttc
     3541 cagccgtatg tatttattgg ccgtatctgc ctctttggtg cctgatacct tttccgtcag
     3601 gcttgatcct ttaatggcta agatatcagc cttgacttcc aagagttcct gctccacttg
     3661 ccggagcagg aattcttggg tccgaatacg attaagatat tcctttgacg tcattcgcat
     3721 tctcccatgt tgatcaagtc aataataatc gatttctcgt cctgatccac cacatacccc
     3781 catgacttga tagtgtcc
//
LOCUS       DAWDXZ010000117         3798 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5715_length_3798_cov_7.076409, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000117 DAWDXZ010000000
VERSION     DAWDXZ010000117.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5715_length_3798_cov_7.076409"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2332..2341
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          815 a         1061 c         1016 g          896 t
ORIGIN      
        1 ctttaaacat cataagtcca gcactccttc tgccacatca gccgtttcgt cgcctttatc
       61 gaccatggcc gctgctttct ggcgggcata atccaaggcg tcttcgcagt ccttggacaa
      121 tttaacggcc ttttcatata aatcgaggga ctgtttaagc gtcaaatccg gcgcttcgag
      181 gtcgcggacg agctgttcca gtttctcgta attgctttca aaatttttca gtttatccgt
      241 cgttcttgcc atatcgcttc acctctttca cgtctgtcac aatcgtaccg tcgacgagct
      301 gtatttcaag gggtttgtcg gcggctacgc tgtcgatggc ggtaatgatt tttccgtcct
      361 gtacgagctg gccgtagccg cggcgcacga ggttttccgg attcaggccg tccaggcggc
      421 cttttaaggc cagaattcta ctctgtcctt gttcgagctg ccgctgccac tgatggccgg
      481 caccttccat gagcaaggcc aattgagcct ttttcatttc caaaaagcgt tcatagtgcg
      541 gcaggttaag ccgaccggcc gcggcgtcga cgcggcgctt ttcactgtcg atacgccgca
      601 caatgatgtc atacgcccgt tccgccaagg cggctaagtc gagctgggct tcctcaaaat
      661 caaaaaaagc cagttctgcc gcatgggtcg gtgtcgccgc ccgtacatcg gcggcatagt
      721 cggccaaggt cgtatccgtt tcatgaccga tggccgtaat aaccgggacc ttagccccat
      781 agatggcctt tacgacagcc gggctgttga agcaccacag gtcttccatc gatccgccgc
      841 cgcggccgac gatgagaatg tccaagtctt ttcgcttgcc catctcatcg atggcggcag
      901 caatggtctt atcgacgcct tctccctgga cggggatggg tcggaggacg atgtcggcaa
      961 agggattccg ttctccggca atgcggcgga tatcatggag aacagcaccg gtcgctgaag
     1021 tcaggacccc gatgcgccag ggaaagcgag gcagttcttt tttatgggaa ctgtcgaaat
     1081 agcccttggc ctccaggtct ttcttaatct gttcgtactg agcctggagg gatcccgtcc
     1141 cctgggaaaa gagctgttca atgataaagg acagggcgcc gcctttttca tagacgttga
     1201 cggcgccgat gacgacgacg tgatcccctt cgcgcagggc ccttacggca gccgtaaccc
     1261 gactgcgaaa gacaatagcc cgtagagaac aggcttcatc acggatattc atataataat
     1321 ggccgttaga tgcctgcttc aaaccgatga cgttgccttc catggcaatg ctctgcagtc
     1381 ggtaatcctt ctgtatatcc tctttaatgc ggcggacgac gtccgtaacc gatgcatatt
     1441 tcatcatttc ttcccttcct gtagggccgc ccaaaaagca gctcccgaag cattgtcggc
     1501 actgaatccg tcctcggcaa ggtcgagtcg gatgtgcttt tttttgcagt atgtgcccag
     1561 ttcttggcgc agataggcat tggacatgac gccgccggcc gcgacgaggt gggtcatgcc
     1621 gtcagagacg gcgtaatcga ggaggcgatg aagtcccctc caaaccgtag ccagcgtcca
     1681 gcggcagagt cgaggtatat cggcgtcccc gtcctttaaa aaccgctgac cctgtgattc
     1741 gggaccggaa aaactgactt caccgtcttt aaaaaagacc gaggctcctt tgagggttcc
     1801 cgaaaacgac tgtgccatct tatcgacgtg gacaccggcc ggaaatggca gattcatggc
     1861 gacgcccatg cggtcgatga actggccggc gctgatgtcg ctcgtaccgc cgatgcgggt
     1921 gatgtcaagg cctttatcgt cggcaacggc ccggacgagc tccgtcgtac cgccagagag
     1981 atggataccg taaaaaggct ctttttcgat tttccccaaa ctgcgaagaa cagccagcaa
     2041 atgattttcc tgatggctca agcggtataa gggtacctgt aagacggccg atagagcccg
     2101 ggcatacccg aggcctgcca aaaaagccgg catatacgag tcgtcccggc gccgtggctg
     2161 gtcggtcacg gcaatagccc gaatccggcg cgtcttcagt actgctgccg cccgttcaaa
     2221 gagaaccggc aggttgcgcg tatgctgata gaccatgttc gactgggaca ggccgcggcc
     2281 gcctttggga accgtcagga tgatccgttc atcagcagcg acgccatagg tnnnnnnnnn
     2341 ngggacgtcg tatagcagct ggtatcgata ccgagatagg tatccatcag tctttcgacc
     2401 ttacgtaagc gccgaggatg ccgttgacga atttcgccga cttatcgccg ctgaattcct
     2461 tggccaggat aatggcttca ttgagtacaa tggacggcgc caacgtttct ttcgggtaga
     2521 ccagttcgca cagagccaag cgcaaaatat tgcggtcgat gcggttcatc cgttcaattt
     2581 cccagccttt gacgaaggga cggatgatgt cgtccaattc agcacgatga gccgtcgtcg
     2641 tttcgacgag gtactggctg tacgtatgcc gttccgattc actgagttcc ggaccgtctt
     2701 cagtcagcat atccgtcgtg atgaaatcgc tttccatgat ttcatgttcg tggtactgaa
     2761 tgtcgttttc ttcgtgaaat tcacgggaat ataagagttc gagcgcctct tggcgcgctt
     2821 tatgtctgct cacaaaaaac ctcctgttgg taactaatct gccggcggca gtaccaaatc
     2881 ttctatagta acagaaatat caaggccctt cgggtcggcc atatcctgca cagccccggc
     2941 ggtacacttt tggatggcga gggccaactc atgcaggtta acaccataga cagcacggat
     3001 gcccaagcga agggagacac tgccgtcttt tacggaaaca ctgcagcggg tcacggccgt
     3061 gacgccgtcc acgtctttgg ccgccgtttc ggcaatgttg cgcagggcct tatcactgac
     3121 ggcataagca aagtcctgat tgtcatccat cttgaccggc ctcctcgaaa accacttgct
     3181 ggacgatgat gtgaacggcc gcaacggggc aggccgtcgc cttttcaacg gcgtctttga
     3241 ccgtcttttg gacttccaag gcgacgtcag ggatgcggca gccatagcgg atgagcagcc
     3301 ggacggtcaa ctcaatcacc tgtccgtcaa tggagacatc gacaccgtga ttaggccgtg
     3361 tccggcccag tttttcagcc gccgcatcgg caatattcgt tcctaaagca gcgactgacg
     3421 gaaccttggc cgccgtcagc gaagcaatgg cggcaatgac ttgttgggaa atgtgaatac
     3481 tcccccattc attgacttct gtcgtttcca tggacatcac ctctttcatc caaaaaaagg
     3541 ggctgtcgca cgcgcgaagc cccttgaata ccctatgcta gcggctaaca cggtaatccg
     3601 ttaaccttaa gctctgccga tatactggcc ggttctcgta tcgatttgga gaacttcgcc
     3661 ttcattgata aagagcggta ctttgacgac ataacccgtt tcaagcgtgg ccggtttgct
     3721 gccgccggta gctgtatcac cacggacgcc ggggtcggtt tcgatgactt taagttcaac
     3781 ggaagccggc aaatcgat
//
LOCUS       DAWDXZ010000118         3794 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5725_length_3794_cov_8.309441, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000118 DAWDXZ010000000
VERSION     DAWDXZ010000118.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5725_length_3794_cov_8.309441"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    607..616
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3254..3263
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1007 a          978 c          987 g          802 t
ORIGIN      
        1 ttagataaaa ataaggagat tgatgaaaaa tgaaggtaat tttattagaa gatgtaaaga
       61 aactcggaaa aaagggggac atcgtcgaag tctccgaagg ctatggccgt aacttcctcc
      121 tgccccgtaa gttagcagcg ccgggcacgt cggaaaatat caacgatgcc aagcagaaga
      181 aagccgcagc tcagcataag gcccaggtag catcagacga agccgtcatc ttggccagtc
      241 agctcaagaa agtagagctc acgattcccg ttaaagtcgg tgaaggcggc aaagtattcg
      301 gagccattac cggaaagaac atcagtgacg ctgcgaagga acagtatgac atcgaccttg
      361 acaagaagaa agttgaaatc aaggatccga ttaaatccgt cggcgactac gatgtcacca
      421 tccgtgttca tccgaccatt accagtgtca ttaaaataca cgttgtagaa ggctgattca
      481 ctagccgcaa gaggttacaa tgaaagaatt attggataaa ctgttacagc gccataagta
      541 cttagaacct acggcggagg aagaactccg ccgccaggtt aatgtgcttt atggcgcttt
      601 tactggnnnn nnnnnntgcc agcaaatact cagccttgtc gtacattcat tctgaagacc
      661 cccgccagcg tctggtcgga ctacagcgtc tcatttacga agacgtatcg tacgataagg
      721 tgcccggtga cgacgaagtc atcaatgttc tcaatgagct tgaaatggtc cttgccgaaa
      781 tgctggcccg tcaggccgtt gaggaacgac tggaaaagaa aattactcag cgcatggacg
      841 aaaaacagca ggaatacgtg cgggaaatca agatggaact catcaaagaa gaactgcacg
      901 atgtagaaac gcagcagacc aagcgcaagc tcaaagaatt ggatgacctc gaaaaggtcg
      961 aacttacgga atcggtcatg gaacgggtac ggccgaaacg gctcagtgcc atcgtcggtc
     1021 aagaacgggc cgtcgaagcc atgcagagta agctggcttc cgtctatccc cagcatctgc
     1081 tcctctacgg accgcccggc gtcggcaaga cgacggctgc ccgcatcgtc ctcgatgaag
     1141 caaagaaact ggcctttacg ccctttggtc ctaaggctcc ctttgtagaa acggacggga
     1201 cgacccttcg ttgggattcg cgggatatga ccaaccccct catcggctcg gttcacgatc
     1261 cgatttatca gggggctcgc cgcgatttgg ccgatacggg cattccggaa ccgaaaccgg
     1321 ggctggtcac cgaagctcat ggcggcatct tattcatcga tgaaatcggt gaaatggatc
     1381 cgatgctgct caataaattg ttgaaagtca tggaagataa acgggttcgc ttcgaatcgg
     1441 cttattacga tgaagacgat cccaacgtcc cccaatatat ccgcagactc tttgccgaag
     1501 gggcaccggc ggatttcatt ctcatcggag ccacgacgag agacccgtcg gaaatcaatc
     1561 cggccattcg ctcgcgctgt gcagaaatct tttttgaacc cctcattccg acggacatcg
     1621 aaaaaatcgt ttctcatgca gcggaacaac tgggggcgtc ccttgaagac ggcgtcgcta
     1681 aatctattgc cgaatttacc attgaagggc gaaaagccgt caacattctg gccgatacct
     1741 acggattggc cgtattccag tcgggaaata aagatcaggt acgcattacc aaggaccatg
     1801 tccgccgcgt cgcccagatc agccgcctcg ttccctatgt gacggaaaag gccagcaata
     1861 cgccggtcgt cggcaaggtg ttcggccttg gcgttgccgg gtacctggga tctgccattg
     1921 aaattgaagc cgttgccttt ccggcgcgag aaccgggaaa aggatactac cgattcaatg
     1981 atacggcagg ctccatggcc aaggattcga tgttcaatgc ggcagctgtt atgcgccgcg
     2041 tgacggggaa agaactttcc gattacgacg tcaacatcaa ttttgttggc ggcggcaata
     2101 tcgacggccc gtcggcagga tgtgccatta cgacggccct tatttcagcc gtatccggcg
     2161 tgcccgtccg tcaggacatg gccctcaccg gggaaatctc tatccaaggt caggtcaaac
     2221 ccgtcggcgg cgtctttgaa aaagcctacg gggcctgcca agccggcatg aagggcatca
     2281 tcatccctaa ggaaaattct catgacattc cggaacgcca tctgggactc gatatccatt
     2341 atgccgctga tattgacgac gtactgaaat tgatgttggt caaatagggg gaatcatagt
     2401 atggaacagc gggtgccacc gcagaatgtc gaagccgaac aagctgtcct cggagctatg
     2461 ctcctaagcc acgatgcggt catcttggcg atggaacgac tgcagtcgca cgacttttac
     2521 cgggatgtcc accgcgtcat ctttgaaggc atggaacatc tgcaccggga aaataaagaa
     2581 atcgacgtca ttaccttaac cgaagagctg cggcgcatga agcgcctgga tgatgtcggc
     2641 ggcgtcgaat acgtcctgca gctgcccaat atggtcgcga cggcagccaa catcgaatac
     2701 cacgccaaca tcgtttctga aaaagccttg gcgcggaacc ttatttcgac gtgtaccgaa
     2761 ttgacgactg aagcctacga tggagaaaag gaaccggagg acctcctcga cgatgccgaa
     2821 cgaaagattc tgcagctgtc ggatacgaaa aaccgcggcg actttgcctc tgtcggcgcc
     2881 gtcgtcgaaa cgaccctcga taaaattacc aaactttatg aaaataaggc cggtcttacg
     2941 ggtcttccta cgggatttcg cgatctcgac cgcatgacat cgggccttca accgtctgat
     3001 ctcatcctcg tcgccgcccg gcccagtatg ggtaagacgg cctttaccct gaacattgct
     3061 cagaatgtcg gcgtccgtca gcgaaagacg gtggccttct tttccctgga aatgtctcag
     3121 gaacagctcg tacagcgtct gctctgccag atcgctcata tcgattcaca aaagctgcgt
     3181 acgggtcagc tcaacagtga tgacgaatgg acgcgcctta ccgatgcctg cgacaagctc
     3241 tatgaatcgc cggnnnnnnn nnnaaatgcg ttctaaggcg cggcggctaa agtcagagca
     3301 tggcctggat ctcattatcg tcgactacct gcagctcatg caggggcgta actcggaaag
     3361 ccgccagcag gaaatctctg aaatttcccg atccctgaag gccttagccc gggaactcaa
     3421 agtgcctttg atcgccttgt ctcagctcag ccgaagcgta gaaagccgtc aggataagcg
     3481 gccaatgctc agtgacctcc gtgaatcggg ggctcttgaa caggatgccg acatcgtttc
     3541 cttcttatat cgagaagact attacgataa ggaaacggaa aatcagcaca ttacggaggt
     3601 catcctggcg aagcatcgta acgggccggt gggctcggtc aaactttact ttaaaaatga
     3661 atttactttg ttcctcaact tggatacgca gcacgaagga taacgtgctg cgttcttttt
     3721 gaagagggaa agaaaaagag gttaattgca ataccaatta ggacactcga gacaattcgg
     3781 cctgaaattt agat
//
LOCUS       DAWDXZ010000119         3765 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5756_length_3765_cov_8.310243, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000119 DAWDXZ010000000
VERSION     DAWDXZ010000119.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5756_length_3765_cov_8.310243"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    693..702
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1850..1900
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          876 a          988 c          931 g          909 t
ORIGIN      
        1 gtgctccttg gcacatttcc aaatcagttc aaaagagaac tcaaaccgct gaatcaaggc
       61 gtcccgctca atttcattca aggtgtcacg ggaggctaat tcgtgaaatt tctccaaggc
      121 cctgacggct aatcgatacc gttcggttat gctttccaca aaatgccctc ctctcgaatt
      181 cgccggcata aagcggctcc ggcgtgatgc atatccacca catcgacgtg acgaggaatc
      241 gtagattctt ccaatcgttc tctcagcaac gtaatttcat aggaaatatc gtcccggcta
      301 tcgatggcta tatcaatatc ggagctccgc ttctgttctc ctcgtgccca ggacccgaag
      361 agataaacgc gcacatctcg ttctttgaaa tgctgcaaaa ccacttcttt tacgcgctgt
      421 acaggctctt catagatgct gtagactgtc ataataatcc ccatcttccg ataaatatct
      481 ttctcgtttc actatagcaa aggttcctta acttcgcaag ggattggcag gacagacgcc
      541 agacagcaaa aggctgccct gccgatattg gcgggacagc ctttccaatc cgggatgttc
      601 ccggcttttt ataactgttt gatgaggttg accatttcga tggcaccggt ggcgacgtcg
      661 aagcctttgt tccccgcttt tgtgccggcc cgnnnnnnnn nntcttcagc gatgcctgag
      721 caatgccttt agccgattcg ccgcaaacga ggtcgtagtg gctggtagca ccgcggatga
      781 cggcaccgag gcaaatgacg gcgtcgtatt tacccgattc agccattttc tgagcaatca
      841 gggggatttc aaaggctccc ggaacccagg ccgtcgtaat atcgtcatcg ttgacgccgt
      901 gacgatggag ggcatcgtag gcgccgccga gcagtttgct gcaaataaat tcattgaaac
      961 gagcgacgac aataccaaac ttcgcaccag aaccgattaa ttgaccttct ttaatgtgtg
     1021 ccataataac aacactccta ttctaaatta tatattgaag ttaaaatcca ttttccagca
     1081 gtttgtccaa cgtcaggcct gatgaggact gaggcgaacc gactgtgaat cgatggacgt
     1141 atttcccaat gacatctgtt tccaaattga cgatggctcc cggatgttta tccatgaggg
     1201 tcgtattggc catcgtatgg ggaatgatgg aaacggagaa ctgactgtta ccgacggaaa
     1261 cgacggtcaa actgatgccg tcgatggtaa tcgacccttt ttcaacgatg aagatttcca
     1321 tatctttcgg aatggaaatg gtgatgaccc gggcaatgcc gtcattggca atgcgggcta
     1381 cgtgaccgac gccgtcgatg tggccgctga cgatgtggcc gccaagtcgt cctccgacct
     1441 gcatggctcg ttccaggtta accttagaac cgctcttaat cgtgctgaac gacgaacgac
     1501 gcatcgtttc atgcatgacg tcagccgaaa aggtcgaatc cgtcaaatcc gttacggtca
     1561 ggcagacgcc gttgacggca atactgtcgc cgagctgtgt tccttctaat actttatgag
     1621 ccgcaatcgt caaccgaata gagtcgggac ggcggtccat atgggcaata gcccctaatt
     1681 cttcaatgat gccagtgaac ataggcaccc tccctattgg cattatatgc cgtaagcaat
     1741 acgttatttt catgcatctc tacagaatcg tagataagcg gcaggcattc atcgagctca
     1801 tcgacgccat aaccgccgac cgacggcagg gccccgacac cgccgataan nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn catagacttt atcgacgagg
     1921 cgggcatcaa agaaactgcc ctgaacagta cttccccctt cgacaaggat actggtcagc
     1981 ccttcttcat ggagtcgttt caaggcttcc ggaaggttga tggactgatt gtcttgaagt
     2041 agaggtgcca aaatgacccg aacgccggca ttttttaaag ccttcatctt ttcaacgggg
     2101 caggccgacg tcgtcatgac gatggtaggc gtcgtcttat cggttacgac cagggacttc
     2161 agcggcgtcc gtcccttagc atcgaggacg atgcgcaccg gctggtgcgg tgcctcctga
     2221 ccttctcgtt cgatacggca ggtcaacagc gggttatccg ccaagacggt gttgatgccg
     2281 actaaaatgc cgtcgtaaat ggagcgcaga tagtggccgt aatttcgggc ccattcgtta
     2341 gtaatccatt tcgaatggcc cgtccgtgat gccgtgcggc catcgaggct ttgggccagt
     2401 ttaacggcaa tgaaaggttt ttggaccatc atgtttttga taaatacttc gttgagctgg
     2461 gctgcttctt tcgacaacag gcccacttca acggcgatac cggccttgcg caggatagcc
     2521 gctcccttac cggagacgcg gggattgggg tcgagcatgg ccatgacgac cttgccaacg
     2581 cccttttcgg ccaacgtccg cgcacacgga ggtgttcggc cgtaatgggc gcaaggttcg
     2641 agggtcacat aaaccgtcgc tcccttggcc ttttccccgg cctctctcaa cgcccacact
     2701 tcggcatgag gggtgccggc cttatggtga tagccggtgc cgacgatttc accatctttg
     2761 acgatgacgc agccgacggc cggatttggt gtcgtataac ccagagctga ttttgccagt
     2821 tccagggctt ttctcatata ctctgccggt tccaatatat tccttccctt cttcttggat
     2881 ttgcaggcgg gaaaacgaaa aaaggctatg gatgaaaaat ctccatagcc ctttgagcac
     2941 aacaaaaaaa gacactgtcg tctgtctttt cccatccaga ctgtactgtc ggtaccgaaa
     3001 tttcatcggt tcaacccttg cgggttagcg gactgtcacc gccggtcagg aattgaaagc
     3061 ccagctttct caccttgcct caaagactat ttctattctt cttttcatgt acgattttag
     3121 cacatgaaaa ggagaaatac aagtccttat cgcaaggacg caatggcggc ttttcgatca
     3181 gccgcaccaa atacagcact gccggcaacg aggaaggtgg cgccggcatc tttgaccatc
     3241 tgagccgtct tatcgttgat gccgccgtcg acttcgatga cagcatcggt atttcctgca
     3301 ttctggagga gttccttggc ctggcggatt ttgtcaaggc tataaggaat gaacttttga
     3361 ccgccaaaac cgggattgac gctcatgata aggatgaggt ccaattgtcc ggcaacacag
     3421 gacaaggtcg atacaggcgt cgccggattg agggctacgc cggccttcat cccggcttca
     3481 tggatttgct gcagaacccg gtccaagtgg acacaggctt cttgatgaac cgtcatatat
     3541 tggacgccgg catcggccag gccgtcgaca tacgcttcgg gattggtcac catcaaatga
     3601 gcatcgaagg gaagtttcgt aaccgggcgc aaagccttga tgacgggcac gccgaaggtg
     3661 agattgggga caaaatggcc gtccatcaaa tcgagatgga tacagtcggc ccccttctgt
     3721 tcgacatctt taatttcttc agctaaccgc gaaaaatcag cggac
//
LOCUS       DAWDXZ010000120         3755 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5782_length_3755_cov_7.717297, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000120 DAWDXZ010000000
VERSION     DAWDXZ010000120.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5782_length_3755_cov_7.717297"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1012 a          789 c         1035 g          919 t
ORIGIN      
        1 tgaactcggt gctgatttga ccttgacgga aaccttcctg cgcatggggg tagacgaact
       61 gtcggtcagc cctccgtctg tattgccgct gcgtgaaaaa gttcgctctt tggatctcag
      121 taaataatct atcaaagcgt tgctccttcg ggagcagcgc ttttttgata ttttagccta
      181 aaatcctctt tacttttttg tataaaatgg tataataata aaaacaaatt taatttttat
      241 tcattttaat ggaggaattg gcatggatat gaaaaaaatg ggtactgcat tgcttacagg
      301 tgttttggcg gcgtcactct tagccggctg cggcagcagt caaaaagaag caggcgatgc
      361 caataagccg cttcgagtcg ctaccaatgc gaccttcgtt ccttttgaat ttaaagacag
      421 cgatcagtcg gccgaataca aaggctttga aatggatttg atccgtgccg tcgccaaaga
      481 aatgaatcgc gatgtcgaat ttaataatat tgccttttcc ggtatcattc ctatcatcca
      541 gcaaggggac atggacattg ctgcgacggg gatgacggtt acgccggaac gggctcagaa
      601 agtcgctttt gcagctcctt tctatgaatc gaaactggtc atcttaacgc cgaagaacag
      661 cggtatccat tcggcagcgg atctccaggg caaacagatt gccgttcaaa tcgggacgac
      721 aggggctaaa tatgctgaag aacagggata caatgtcaag cagttcgata ataactcgga
      781 agccatcatg gaactccagg ttggcggctc gccggcagct atcatcgata agcctgttgc
      841 cgattacttc ctgacccagg acggaaagga taaattcgat gtcatcgata tcagcaatac
      901 gaagccggaa tatctggcct ttgccctcaa taaggataat aaggaactct tgcagcaagt
      961 aaacgaagct atggaaaaat tgaagaagag cggtgaattc cagcagattt acaaaaaatg
     1021 gttcaacacg gatatgccgg atctcccgac cagtgccgat gccgtcttga aataaacggt
     1081 aatgaacgca tccttcttta gggactatta gcgataaaaa ataagcagcc tttaagggct
     1141 gcttattttt tatgataccg gtatttttgg atgcttgcga aactatgcaa gggtataaat
     1201 ctttacttcc ggatctcctg tccaaagggg ctgaaggccg acgaagacgt cgtttttgaa
     1261 gaggtcgctg gaaagataag cctgggcgtg ttcggcactg tcaaagccgt ggagtacctg
     1321 tacgtcttca tcgcggatga ggaggtcttt ggtcaaggct ccttcaatag tcgtcaggaa
     1381 gggctggcgg tagtccgtat atactttggc ggcagccggt cgattggcgg catcgatttt
     1441 catggtaatt tctaaataag cctttacgtt catagtatgt tcctcccttt cggttttctg
     1501 tgagctcttc ttcattatag cggccctact cggccgggag aagatataag caaatttatg
     1561 agtcactatt cattttaata gtaacttgaa gggataaaag ggagctacta taataaaatc
     1621 agcatcaaga aaggtggcgt gaacggtgta taagcctaaa ttagaaaagg atatccgatg
     1681 tcccttagaa tatggactgt ctgtttttgg cggtaaatgg aaatcgagga taatctgtgt
     1741 gttagctgaa aacggaagac tgcgatacag cgctatccgc agggaaatac ggattgacgg
     1801 ccaaggggta ctcatcggtt cctatacttc aaagtatctg caaatggtcc ggagcttatc
     1861 atcgggagga taatgagcat acgatgcgtc agtgtcaaaa atgtgattac aatcagcgga
     1921 ctgactgagg gagacctatg tgttcttata atccgagcag gatttttcaa cgggtacagc
     1981 cgataaaaaa agagagattg tcaccgagtg gatgacgatc tctctttttc atggataccg
     2041 tgcgtttttg caggatatgt catttaactt ttcagttcag gcattttgat gggtgtctta
     2101 tcgacgaggc ggacaatcag caaggtgatg atacagatga cccattcaaa gtagatgaca
     2161 tggggcagca ggcggacagc cgggcataac tgccagagaa tgaggccggc gatgccaacc
     2221 aaagtcgtcc aaaaggccgt gtttttacgg cacagggacg gtgcacagga ataccaaggt
     2281 aaaggctgtc gtaaggctca ggccaatgag cataaccttt acgatgccga cggcatagaa
     2341 ggccatcagc agggtgatgg cgccaatgat gacgatgatc aggcggttgg ctttgacgta
     2401 ggtcatgtcc gacatattcg gcttgataaa acgtttaaac acgtcctgag agatgagcgt
     2461 gccggcgccg aggaggatgg tgcaggccgt cgatacgtca gcggcccaaa gggcggctaa
     2521 ggtcatcccg gaagagaaag gatcgaggct catgatgatt tgcggcaggg ccagggtcgg
     2581 attgatatcg gggaactggg ccttggcagc caggcctaag atggccgata agaagccgat
     2641 agggaagatg agcaggccgc cgaggataaa tcctttacgg gcttctttgg cgcttttggc
     2701 accgcaggcg atctgtaccg gcccttgggc ggtgatggcc tgggtggtca tgacgatgat
     2761 ccagcccatg aggacggcga aggacaggcc gcccatggga gagaaccagt tgaagtccgc
     2821 tgccggaagt gctgcgttga tgccgctgat gccgccgtca cggatgagga tgcgaattag
     2881 ggaatagatg atgccaaggt aaatgacggt aacgcttaag atattggaca ggcccgacga
     2941 ccacaggcct ccgatggccg tgatgccgac gaagacgatg gcgctggtaa tcatgccgcc
     3001 ctgcatggaa aagatatccg gcatgagggc gtgcaggatg gagccgccgg cgacgtactg
     3061 cagggacgtg atgcagagca tgatgagaga caagccgatg acgctgatga tgcgggcttt
     3121 tttatcgtag ctccgttcaa agagttcggg aatggtcgta cagttcagcg aacggtattt
     3181 gccggcagcg acgaagccca tgacgatggc cccgatagcc caggcggcat tgtaccaacc
     3241 ggcagccagg ccgactttga cggcactttc ggcgacgccg acggtggaag ctgcgccgac
     3301 ggccatgccg gtaatgctga cggcgaccag gccggtggaa aacttacggc cggcaaagag
     3361 atattccgtc gggttttgac tggcccgatg tttgacgtac atggaaatgg caaataataa
     3421 gatgatgtat aagatgacaa tagcaagttg aatcgacatg ttccttgtag ctcctttttt
     3481 tggtattttg caacattggt tccttacaag ggaacaacgt gtgacaagaa ttggtaatat
     3541 tataggataa aatgatgaaa aatacaagga ttttatcgat gaatggactc ttttttttcc
     3601 gattttgcag ggaccattgc atgaggcgca taaatatggt atgatacggc cgaggcaaaa
     3661 gatagcatca cgcaatgtga gttatacttt ttttatgtct actatcgatt cattatctgc
     3721 ttggatctga ttcagaccga gacaggaagg attgc
//
LOCUS       DAWDXZ010000121         3752 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5786_length_3752_cov_8.083581, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000121 DAWDXZ010000000
VERSION     DAWDXZ010000121.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5786_length_3752_cov_8.083581"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1637..1646
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          853 a          929 c         1035 g          925 t
ORIGIN      
        1 cgcgacagct ttttatctta tttaccgctt tcagcgatgc agtcgttgat atcggcgatg
       61 agggcatcga cgtcgatgcc gtgagcaccg gcaccctgac cgatggtttc aaagtgagca
      121 gccatgcagc cgacgcagcc aaggccgtac tgctggaaga tttcgatgat ctgcgggtgt
      181 ttctgaacgg cttcaataat acccgtatct ttggtaatct gtgccatagt atattcctcc
      241 ttcttcgtta aatacaaccc ggatggtttt gaaggaacgt ccggattgag tctattatat
      301 cacacttttt ttagttgtgt gaagaaatgc ttcgttcctc taacagttga ccgtcaggga
      361 gaaaactgcg gaaggtcagt ttgtctgccg tcgcttctaa ggtcatgtag ttgtctgttt
      421 ccggctgagg cgcgacgtat tcatcgaggc tgtgctgctt ccagaggctg ggatagcgga
      481 cgttgccggc gacgccggtg atgatgtata aggggccgct ttcatcgcgc tggaagttct
      541 tgatatggcc tcggtcgcgg taggtgtgga gatgggccga caagacggca tcgacgccgt
      601 actggtcaaa taagggcatc cacagccgtc cttctgcaga gaagccttct tcacgcggtt
      661 ccgggcggtt ggcaaagccg tactgcaaag gatctttgtg catcaagacg accttccatt
      721 tcttcgtcgt cttggccata tcttctttga accaggccac ttcatcgtca tttaaggtcg
      781 gctcgaagtc ggccagttcc tgggcctggg tgttgaggac gacgaagtgg atgtcgccgt
      841 aatcgaagga atagaattgg ttctggtatt tattcatgtc gatatggggc agggcgaaaa
      901 gatgggtata ggcttcgggc atgcgcatct tccagtccgt gttgtaggtc tcgtggttgc
      961 ccatgatggg gacaacgggg atgcgggcga tcagcgggcc gacggcgtcg aaccaggcat
     1021 tccactggta atgggactgg ccgttatcga cgaggtcgcc catgttgacg aagaactgag
     1081 catcctgatt attctgccaa gctgcttgcg ccagggaggc ccagccgctg tagtcgctgg
     1141 actgggaatc ggggaagatg agggctttga aactgctgcc ctcagccgta cggaaaggcg
     1201 tccaagcact gcgcttgtcg ccgtagccga cgcggtattc ataggctgta cccggcgtaa
     1261 ggtcggtaac cgttgccgag tggatatatg tcgtcacgcc gtcatcgctg aaggcgtcgc
     1321 tggaagcggg aagggccata atcgtattgt cgtcgccggt cttgcggaat tccacgaccg
     1381 gatcgtcttc ggcgtagtcc gactgccaca taaaggtccg cgacgtcgtg ctgtcagccg
     1441 taatgacctg gcggatattc gaggcatcta ccgattcggt cggcaagtag tgggccgctg
     1501 ctgctttcgt ggctcgaatg ccctgtttgg cgacggcctt cgttcccggc agccaaaagt
     1561 aggctgctgc ggctgcaatg gccaggacgg cgacgatggg gatgattatt tttttatggt
     1621 tccatttcat agaattnnnn nnnnnntcat tattgtagtt attatagact atggagtcaa
     1681 ctctaagtca agaaaaaact tgacaaggga gggccttcga tataagatag atgcatcaaa
     1741 taggatatat aacgtcgatg agcgggagca agtaccgtca tgatacccct tgacagcgag
     1801 ccggtggcag tggaagtccg gtaagaggcc gtgatcggga atgggcccgg gagatgcgga
     1861 ctgaacgaag acagtaggtt ccgccggaat tttcaccgtt accttgaaaa gatgcatcaa
     1921 caggtgcatt agagccgggc catatggcca accagggtgg taccgcggag ctgactttcc
     1981 tttcgtccct atgtataggg gatggaaggt tttttttatg aatcaggagg taccattatg
     2041 gcagttattt tttccggtat tcagccaagc ggcaatctta ccttaggcaa ctatttaggc
     2101 gcactgcgca acttttctaa aatccaggac ggcaacgaat gttattactg cgtcgtcaat
     2161 cagcatgcca tcaccgttcc ccaggacccg aagcttttgc atcagcggac gcgggaactg
     2221 gcagctatct atttagcttc cggcctcaat ccggataagt cgacactctt cgtccagtcc
     2281 gaagttcccg aacacgccct cttaggctgg atgatgatta cctcgtccta cgtcggcgaa
     2341 ttggaacgga tgactcagta caaggataag gcgtcgaaac agggcgattc cattccggcc
     2401 ggtctcttga cctatccgcc cctcatggct gccgacattc tcttgtatca gacgaatttc
     2461 gtgcccgtcg gcgatgacca gaagcagcac ctcgaaatta cccgcaactt agctcagcgc
     2521 ttcaaccgcc tctacggcga agacatcttt accaggacgg cacgcgggtc atgagcctcc
     2581 aggaaccgac caagaagatg agtaagtccg atgataacga aacggctacg atttatctcc
     2641 tcgacgaacc gaaggctatt gaaaagaaaa tcaagcgggc tcagacggac agtgaaaact
     2701 ccgtccatta tgacaaagat cagaagccgg gcatttcaaa cctcatcgaa atcctctcgg
     2761 ccctgaccga ccagacccat gaacaggtcg aagcggctta cgtcggtaaa ggctacggcg
     2821 cctttaagaa ggacgttgcc gaagccatca tttcgacgtt ggaaccgatt cagcagcgct
     2881 atcgccagtt ggtcagtgaa ccggaactgg acgacatcct tgacaagggg gcagccaagg
     2941 ctcacgccaa agccagcctt acctatgaaa aggcctgcaa ggccatgggc ttgttccgca
     3001 aataatttca tatatagaca taaaaaaagc gagaccgtga cggtctcgct tttttgttgc
     3061 cataggagga gaggacttac atgccgactt tgttcatcaa ctgctgaatg agagcgcgct
     3121 gctgttcaag ttcagctttc tggctgttga cctggctggc caaagcctgg ttttcttggc
     3181 gaagggtatt gacttccgac gtcaaagcgc tgacatcgac ggacgtaccc tggcgataga
     3241 tgcggtcgcc tttgttgccg aatttgaagg aaacgccgac attgaacatc ttttcatcgc
     3301 tgatcgtcgt gccgaagctg aacatcgtat cagcattggg gcgatagaag gcgccgagag
     3361 ctacggcgtt gtcgcctttg tagttgccga gagcggcaga tgcggagaat ttatcttccg
     3421 ggttgaattc caacggatgg agacctgcca aagcagctgc accggcaccg actttatcga
     3481 cgcggtcgtt caagctgctg acgcggttat ctaagcctgt tacctggtcc tgtaagttgc
     3541 tgacatcact gttcaagttg ctgacgccta aggtatcttt ccagcctttg atatccgttt
     3601 ccgtcaaatt agagccgtcg cggtcagctt tctggtcgag ttccggttgg atagcatctt
     3661 taatggcact gtcgtcgaca gaaatcttat aatttacacg agatttatcg tcgttataag
     3721 tgctttcaat ttttacatta tccccttggg tc
//
LOCUS       DAWDXZ010000122         3751 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5788_length_3751_cov_8.308712, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000122 DAWDXZ010000000
VERSION     DAWDXZ010000122.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5788_length_3751_cov_8.308712"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2497..2506
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3133..3142
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          919 a          876 c         1013 g          923 t
ORIGIN      
        1 tattatactc attttttgtg cattcgtcat atatcaaaat gtacactgga cacggaaatt
       61 agcttgtata attacttttt cgcaatttat attctacatt atggaattgt agcttgaaaa
      121 gggccgccgt ggtataataa aaggcgatta cacaagtaga aagctacttt tgaagggagc
      181 gtcatactat gcatgataat gaaattattg aaggcattat tgccgaattt aaaggactcg
      241 ccaaacatcc gcgtccgtct catcatgaaa aagccgtcag cgattacatc gtaagccgcc
      301 ttcgtgaatt aggcgttacc gacgtcattc aagatgacgt ttacaatatc gttgccaatg
      361 ttccggccag caaaggctgt gaatcctggc cgattacggt tcttcagggc catatggata
      421 tggtctgcgt cgctaaaccg ggcgtcgact tcgacccctt gacgagtgaa attaaattag
      481 tccgtgaagg caatatcctc cgtgccgacg gtaccagcct cggcgccgat gacggcattg
      541 ccgtcgccct gtcgctgtac ctcattcagc aggacattca gcacgggccg cttcgtctca
      601 tctttacgat tgatgaagaa acgggcatga ccggggctat gaatttggac ccgaaacacg
      661 ttcaggatgt aaagtatatc atcaactgtg actctgaaga tatcggcatg ctcggcgtcg
      721 gttcggccgg cagcgtccat acccacttca atcgccccat ccattggcag gcaccgaagg
      781 gtaaacaggc gtttactatt gaagccaaag actttgtcgg cggccattcc ggcgaaacga
      841 tcaaccgcgg caagagcaac gccatcaaag ccctgagttt ggccctgcgc cgaattgaac
      901 aggccggtgt cgtttatacc ctggcctcca tcgaaggcgg cgttgctgcc aatgccattc
      961 cttcccaggc agcggctgtc attgtaacgg ctgacgaaca ggccgaatcg tccattaaac
     1021 aggcggttca ggaagaacag gcccagatcg ctgaagtata cggcagtgtg gaatcgaagg
     1081 ctcacttcgt cgtaacggca gccgatgtac cggctaaaac ctttgctgaa gaggacacca
     1141 aggctgttgt aaacctgctt aacgtccttc actgcggcgt ctttgccatg aatcagatgc
     1201 tgcccaaact gccggattta tcggccaaca ttgggacgat tcgaacggaa gaagacgtcg
     1261 tcgccattca gtacttcccg agggcatcgt ctgacggccg tttacgtgaa tttgccctga
     1321 cactgcccgt ttttgcagag cttacgggct gcactattga cttggctacg ccggaaccgg
     1381 cttgggccga aaatcccaag agtaaactcg ttcccttgtt tgccgacgta taccgtgaag
     1441 aagccggcag agaaccccgc attgaagcca tgcacggcgg ccttgaaacg ggctacctct
     1501 tttccatgaa ccagaatctc gatatcattt ccgtcggtcc gacgacgcac gacatccaca
     1561 gtgccgacga atcggtcgaa ctcgatacag tagcgctctt gacccgcatc gttgctaagg
     1621 ctctgggaaa actcgacaag taatcgattg atgtaaactg aatttaaacg tacccgcctt
     1681 acgggattgt cctgtaaggc gggtattttt atatctgtat gaggtcgtgc aaggtatcgc
     1741 ccaaggcgtc taagttgacg ggcaggcgat tgaggggaat ggctccgtag ccgatgagaa
     1801 tctggtgctg cgggctgtcg tcaggatcgg cccagtaggg tctcagcgag taaacgccga
     1861 cttcattttg tcggaagcgg cggaggattt ctgtttctgt acaatccgta cggagttcga
     1921 ggacgaagtg catgccgccg cctcctccgt ggatgcggac tccttgggga aagttttctt
     1981 ctaagtagct gcggatcaaa gcgtgctttt tacgaaaatc atttcgcagg gcgtttactt
     2041 gcttttcata ctcgccgctc tccatgaagt cggtcatggc cagctgaagg agagaaggca
     2101 cgggagaact gtagaaggga tactctgcag caaaatcgaa agacagagcc gggggcagga
     2161 cgacataggc caggcggatg tccggagcca tcgttttgga gaaggttccc aaataaaaag
     2221 tgcggccata ggtatcgatg gattggagcg acggaatggg ctgctcttca tagcgaagtt
     2281 cactgtcata gtcatcttca atgatgtaat ggccgtcctt ttcggccagc tgcagcagtt
     2341 ccaatcgatg cttgatgggc atgatttgcc ctaagggaaa ctggtgggac ggcgtgatgc
     2401 tgattaaaga cggcgggagg ccttgtaagg acggaaggtc gattcccccc gggccgaccg
     2461 ggacgtagtg catggcatag tccttgtgag aaaaaannnn nnnnnngcgt accgcgatag
     2521 cctggatttt ccatggcaaa ttcgtatttt tccggaggca tgagttgtgt gatcagactt
     2581 aaagaatggt ggtggccgct ggttatgatg acctgttccg ggcggcagcg gacgccgcgg
     2641 aaacgtttca gataccggca aagccattgc cgaagaacga acagcccttc cggcggctca
     2701 tagaggaggg tattccgatt ttcaagatcg gccagggcat tgtttaagga atggcgaaag
     2761 gctcggtttc gatagacgtt tcggtcgata cctccgtagc ggaaatcata atgaggcggg
     2821 cgtttttcga caggcggcgt gaacggttcc gctattttcg attcatacga ctggggcggc
     2881 agggcttcga cgtagaatcc ggagccgcgc gtgctgtaga tatagccttc taattccagt
     2941 tgacgatagg ccgtaatgac cgtattgcgg gaaatttcgt actcttgggc cagcttgcga
     3001 gtggccgtaa gtgcgctgcc ggcagtcagt tcaccggata agatttgctg tttcaggcct
     3061 tcgtaaagtt gcttgtatag ggactgagaa ctctttttat cgatgagcag catcgggggc
     3121 ctccttttag tgnnnnnnnn nnatgcaaga tagcttgcaa gatacagtgc gactgaaaag
     3181 gagaatacga ctatgtttat agaaaaattt tatgactgct tgaaacacga cggcattgta
     3241 gccgtagcca cggcatcgaa agaaggcaag gctcatgtag tgaatacgtg gaataaatac
     3301 atcatcgtta cagacgatga aaagctgctc attccctgct atggtttccg taagaccgag
     3361 aggaacatcg gtgaaaatcc ttacgttgaa attactctgg gaagtccgga catcatgggc
     3421 tacaagagta tggggacggg gttcctcctc atcgggacgg ctgaattcct gtcgtcaggg
     3481 ccgctttttg aagaaatgca cgataagtgc gattttgcca atcgcgtcat ggtctttacg
     3541 ccggaaaaat gcacccagat gatctgatgt aaagacattt acacgacgtt tagtcttcaa
     3601 tggcgtgcgt gtttttatga aggcttcaga ttatcaatgt gattttaacc ataagaaagc
     3661 cctcaaaagc tgccagtgca tggctgcctt tgagggcttg ttgtttttgc aacggtttta
     3721 ttcggtcata tttgatataa taggactgta t
//
LOCUS       DAWDXZ010000123         3705 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5845_length_3705_cov_8.399452, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000123 DAWDXZ010000000
VERSION     DAWDXZ010000123.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5845_length_3705_cov_8.399452"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    379..388
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          931 a          869 c          934 g          961 t
ORIGIN      
        1 tctgttgtag agattggcgg ctttttttgt atgattttct gattattcga tattgtatgc
       61 agggggagtt atgatgacac aacgtaaaaa agacttagtt ttagcagcca tggacggcaa
      121 accggttgaa cgcgtccctt ccggtttttg gtttcatttc ttagacgatg aaatccaggc
      181 caatgctttc gaacatccgg aattgacgga aaaggtcttc gccggccaga cccgctacat
      241 cgatgaagcc aaaccggact tcgttaaaat catgaccgac ggttttttcc cttatgaaaa
      301 tgaaacggct atgaacctta agacggcggc tgattttaaa aagattaaac ccttagccga
      361 tgacgataag tggtttacnn nnnnnnnngc agatcaacta tgcgaaacgc cttacagctc
      421 gttatggcga tgaattggcc atgttcttca acgtattctg tgccggaacg acggtcaagt
      481 tcatgcaggc cgacctcgat gccagtgaag ccttcctggc caatgccgtc aaggaagacc
      541 caaaggccgt acgtcagggc ttggatgtca tttcccatga tttggcgaaa cttgccaaac
      601 ggctgattac cgaaggcggt gtcaccggca tttacttcag ccttcagaat ttattgggca
      661 tttcgccgga gctgtacaaa gaggtcttgg aaccgggaga aaaagaaatc ttagccgctg
      721 ccaacagcgt cagcgactac aatattctcc acatctgcgg ttatgccggt caccgcaatg
      781 acctttcctt atataaagat tacgatgtaa aaaccgtaaa ctgggccgtt gtcgtagaag
      841 gcgtccccat tccggaaggc cggaaaatcc tcaacaatcg cccggcttta ggcggttttg
      901 gaaacttaga ttctgaattg ttgtataagg gctcgaaaga agaaattcaa gctgaaacga
      961 agcggatttt agcagaagcc ggtcgacagg gcgtccttct cggcgctgac tgcaccgttc
     1021 cgcgtgatat cgattggaat cattttgagt gggttcgtga agctgcccga taggcagaga
     1081 gaggaagatg aaaatgaagt ttaccatgaa aaagttaggg aaagccgtat tgacggcagc
     1141 attagtcggt gtcgtagcag cagtccttgc cggctgcggt aaacagagcg attctgccgc
     1201 cggtggcgga gacggccaga aaaaggtcgt caacgtggcc tttaccaact actacgtgcc
     1261 ttatgatttc gtcaatgata agggcgagcc cgacggcttt gaagttgccg tcatgcagga
     1321 agtagccaag aagctgcccc agtatacctg gaagtttaca ccgacctcgg acgatgattt
     1381 gctcatcggc gtcgaatcgg gtaaatacac aatcggtacg aagggaatct ggaaaacgga
     1441 agctcgggaa aagaaataca tcttcccgaa aaactacatt ggcgccagtg tcatcggctt
     1501 agtcatccgt aaagccgacg ctgataagat ccatgacatg gattcctttg cccagtacag
     1561 cggcaaactc gttcccattg cacctcagga tgcccgctac atggtcatcg atacctataa
     1621 taaggaacat cctgacaaac agatcaagct tcagtcgtcg gaagccttcc agattgccga
     1681 cgcttacagc tgggtcatgg aaggccgcta tgacggctac ctcgaagttg aactgagcta
     1741 caagaacaac atcgtcaaag acgatgcacc gtaccacaaa ttcaacgaca gcctgaccta
     1801 catccgctat aaaggcattc cgacctaccc cttgttcaat aagaaagaac agaaattggc
     1861 tgacgaagtc gaccaggcca tcgatgaact gcgcaaggaa ggcaagattg ccgaactgga
     1921 acagaaatac tttggcgaaa gcctgtctaa ctatattacg cagtaaaggc gaaggaggac
     1981 gttttgatgg atacacgtgt gtttaatccg tctgttatct tctcggttat gccggattta
     2041 ttgtcgtatc tgtcggtaac gctgctggta gccgtttcaa gcattgtatt aggttccctc
     2101 ttagggatgg gcctgacctg ggcaaaactc ggacgcatcc gggttttgcg ctggctggcg
     2161 cacgggtaca cttacatctt gaagtgtacc cctcctatcg tgctgctgtt cattgtcttt
     2221 tatggactgc ccaagctgat agaatcgatg acggattatg atattaatga taccaatcgg
     2281 gctctgtttg caatcattac ttttaccctt ttgttcggag cctatatttc ggaagtcttt
     2341 cgggcggctt atcaagctgt gcccaaagga cagtatgaag ccggagtcag catcggcatg
     2401 acgccgttcc agacctttta tcaggtcatg ctgccccagg cggcccttat tgccctgcct
     2461 aatttcggca attcgacgat taacttgctt aaagaagggg ccttggctta tacgatcggc
     2521 ctcatcgacc tgatcggtaa ggggaatttg atcatcgctc aaaattttgg agcctatagt
     2581 attgaaattt atctggcctg tatgatcatt tactggatca tgaccctgat cattgaaaag
     2641 ggatttgctc tcttggaggg cgtcctcgac aagggcgtcc atatgggtca tgcccgggcg
     2701 ggaggaggtg agccgtaaat ggaactcgat tatggtttta tgattgaaac gttttataag
     2761 atctgggacg gtatcggcgt taccttaggg ctgacggctc tgtcactgat catcgctctt
     2821 cccatctcct tttatatggc cgtcattcgc ctgcgggggc ataaactggg gacggggatt
     2881 acgagatttt atatttcctt tgtccgcgga accccgttgg tactacagat tttattgatg
     2941 tacagtctct tgccgagctt ccttaacttc gcattgaagt ccatgggctc gtccttcaat
     3001 atctttgaaa gcgtcaatcc tttctggtat gccgtcatcg tcttttctct caatactatc
     3061 gccttgttag ccgaagtatt ccgctcggcc ttgatgagta tcgataaggg acagatggaa
     3121 gccggcgtat ccgttgggct ttcggaatgg cagacctatc gccaaatcat cattccccag
     3181 gccatggtcg tcgccctgcc ggcggtcagc aatattacgg tgaatttgat caagggcacg
     3241 tcgctggcat tcctgatgac ggtcaaagac gtactggcta tcgggaaaat tgccgcctcg
     3301 tacggctata actatattga agcctatttg gatgtcttcg tcgtttacat catcgtctgc
     3361 acgattgtcc agctcgttta ccgcatagct gaaaaacgaa tcggtgcctt ccgtgccgtg
     3421 taaggagagt atgcctatgt taatgtctat taaaaacgtc cgtaagaagt ttggcggcgt
     3481 agaggtctta aaagacctca atatgaaaat caataaagga gatgtcgtcg tcatcctggg
     3541 ccctagcggt tccgggaaga cgaccttcct gcggtgtctt aactttctcg aacgggctga
     3601 cgaggggcag atggaatttg ccgacatgaa catcgattta agcaagcctt ccgataagga
     3661 gattgccgct gtccgcaaga agacggcctt cgtgttccag ggata
//
LOCUS       DAWDXZ010000124         3700 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5856_length_3700_cov_9.022771, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000124 DAWDXZ010000000
VERSION     DAWDXZ010000124.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5856_length_3700_cov_9.022771"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1696..1705
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          880 a          919 c          936 g          955 t
ORIGIN      
        1 agacacataa aacgagactg ccatagatat ctatgacagt ctcgttttca tgaggccgtc
       61 ctcccgggac agccccgcct aacctttaat acttatttgt cgtatgcctt ttctaagcgt
      121 acatagcctt cataacggtc cttagcatct tcttcggtct tttcaaagag agcttcggca
      181 atttccggga aggtgcgctg cagggacgag aagcgagttt cgcccatgag gaattcgcgg
      241 aagttatctt tcggcggttt ggaatcgagg atgaacggat tcttgccctg tttcttcaat
      301 tccggattgt agcgccagag atgccagtaa cctgcatcga cagcacgttt ttcttcgagc
      361 tggctctgac cctgaccgat cttgaggccg tggttgatgc acggtgcata cccgatgatg
      421 agggacgggc catcgtattc ttcagcttct ttgagagcct tcatgaactg gttcttgtcg
      481 gcgcccatag cgacctgtgc gacatagatg tagccgtact gagcctgcat catgccgagg
      541 tccttcttac gggtcttctt accggctgcg gcgaactgag cgacagcagc tgtcggcgta
      601 gctttcgaag cctggccgcc cgtattggag taaacttcag tatcgaagac gaggacgttg
      661 atatcttcac cctgcgccag gacgtggtcc aagccgccgt aaccgatatc gtaagcccag
      721 ccgtcgccgc cgaagatcca ctggctccgt ttaacgagga actgtttcat atcgtaaacc
      781 tgttccaatt caggtttgcc gtctttttca gcttcgagga gagcgatgac cttatcggac
      841 cgttcgcgag taccttcact cttagccatg ttttctttcc agtctttcag tgcagcctgt
      901 aattcaggac ttgcaacgtc catagccgca tcgacgtact gagccaagag tttccgttcc
      961 gtcttttcag ccagcatcat gccgaagcca tattcagcgt tgtcttcgaa gagagatgtc
     1021 gaccaagccg ggccatgacc ctgttcgttc ttggtgtagc cgcagtccgg tgcactgccg
     1081 ccccaaacgc tggaacagcc gtgagcatta gcaatgatca tgcggtcgcc atagagctgg
     1141 gtaataactt tacaatatgg ggtttcagca cagcctgcgc aagcgccggt gaattcgagc
     1201 aagggctgtt cgaactggct gccgataacg gtgttcttgt ccatcggatt cggtttaatc
     1261 ggcaggttga tgacgtaatc ccagagatct gctctgtatt cttcttcgtc gaagggtttc
     1321 atcgtcaagg cctgtttcgg gcagacatgc gtacagcttc cgcagccgag gcagtccatg
     1381 accgatacga cgatattgta tttgtattcc ggtgctgctt tgatttcctt atcctgatag
     1441 cctttaggag cgttcgcagc ttcttccggc gtcatgagga agggacggat agcagcgtga
     1501 ggacatacga aggaacactg gttacagccg atacactttt cagcatccca gctcggtacg
     1561 aacatggcaa cgccgcgttt ttcttcagca gccgtgcccg tcggccaacg accgtcttca
     1621 aagccgtcat atgcgctaac cggaaggtcg tcgccttcga gagcattgac aggaatggag
     1681 aagtcacgga agaatnnnnn nnnnncataa tcatgttgat gcggccgcca agaccgattt
     1741 tctgtgcaat accaacagcg tcgatgatgt agaattggac gtcgttgcgg gcgatggagc
     1801 gtttaatttt agccggtaat ttttcatcga gttcttccgg tttccatacg gtgttcagta
     1861 agaaggtacc gcccttcttg atgccccgca ggagatcgaa gccgtgcgta taggattgac
     1921 ggtggcaggc gatgaagtct gcgtcattga tgagatacgg catcttgatc ggcgtatcgc
     1981 caaagcgcag gtgagaaacg gtaacgccac cggatttttt cgagtcatat gcaaagtatg
     2041 cctgagcgta catatcggtg ttgtcgccaa tgatcttgat agcgctctta ttcgcaccga
     2101 ccgtaccgtc ggaaccgaag ccccagaact tacaagcttt caggccttca ccagccgtgt
     2161 cgacgttctg gacacgcggc agggatttat gcgtaacgtc atcgacgatg ctcaaggtga
     2221 agtcgttctt cggttcgaag gctttcaagt tgtcaaaagc agctacgacg tcgcccggta
     2281 agaattcttt cgaagcgata ccataacggc cgtggtaaac tttcggagct acgtcagcag
     2341 cagcgaagga agcagctacg ctgtggtaca gcggttcgcc gttagcgccc gtttctttcg
     2401 tgcggtccaa gacggcaatc ttcttaaccg tatcaggaac ggctgccaag aagtgttttt
     2461 ccgaccacgg acggaacatg tgaacgttga tacagccgac tttttcgcct tgttcaacga
     2521 gcagtttaac catttcttca atggtctgtg cgccagaacc catagcgacg agaacgagtt
     2581 cagcatcagg agcaccggtg tagttgaaga gatggtattc acgaccggtg atttcgctga
     2641 ttttcttcat gtagccttcc gcaacttccg gaacgcggtc gtagaatttg ttcaaggatt
     2701 cacgatgctg cataaagact tcagccggtt cgcagaagga aatagcttcc ggattatccg
     2761 ggttcatgcc gcgtttacgg aattcacgaa cggcatccat atcgagaagc ggacggagtt
     2821 tttcataagg aataacttcg attttctgga tttcgtgaga cgtacggaaa ccatcgaaga
     2881 agttaatgat agggatacga caggtaatag ccgttgcatg gacgacagca gccaggtcca
     2941 tgatttcctg aacactggat tcacacatca tgctgatgcc cgtgccgcgc atagccatgg
     3001 catcgtcgtg accggcaaag atcgtgtaac cgttcgttgc gactgtacgg gaagcgacgt
     3061 ggaagacgcc cggcagcaat tcgctggaaa ttttgaacat attactgagc atgatcatca
     3121 tgccctgaga ggacgtgaaa gtcgtcgtaa gggcaccggt attcaaagca ccgtgcatcg
     3181 caccggcagc acctttttca gactgcattt cctgaacctg gacggttgcg ccaaacaagt
     3241 tcttacggcc atgagcagcc cattcatcga cgtgttcagc catcggcgat gacggcgtaa
     3301 tcgggaagat ggatgccagt tccgtaaagg cataggcaat atgggcggct gcctcgttgc
     3361 catccatagt cttaaaaact ttctgtgtca taatactgtt cccctttctg taaaacatcg
     3421 taacttgaaa gaatccgtgt acacttggag tcatcccatt acattgcaag gacaaaataa
     3481 tcactataag ctatgtagct atggtattaa ctagatgact atttgttatc ctcattgtac
     3541 tatttataaa aattttacgc aagagctttc cagtaaaacg ttcatcttat gccgattctg
     3601 tctaaacgca tacatattta tgtaatcgtt gcatggactt tacgatgaac aaagattcat
     3661 catgacgacg attacatttt ataaagaaaa gaatccgtaa
//
LOCUS       DAWDXZ010000125         3681 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5880_length_3681_cov_7.070601, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000125 DAWDXZ010000000
VERSION     DAWDXZ010000125.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5880_length_3681_cov_7.070601"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1469..1615
                     /estimated_length=147
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2086..2213
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          727 a          928 c          817 g          934 t
ORIGIN      
        1 atcttatccg gatcgttcag agcttccttg acccggccga cgtcattgac ttcgttaatg
       61 gcatcaaagg ggcaggctgt cgcacactgg ccgcaatgaa cgcaaatcgg cagatcgccg
      121 gtactggaca gggagtaata gtcgagtacg gtctgcgtgt cggcacaggc tctgcggcat
      181 aaggtacagt tcttacactt agtcacgtcg aactgaacgg cacagttatg ttcatcaatg
      241 gggacgcggc ggtcgatgtt ttcaaagcgg gaatgaaatt caggcatggc gaatactcct
      301 tttcggtctg aaatttaaaa tgctgggtat gatgttggca gcgccaagaa ctatgtaaca
      361 ttttatcaca aactttgtta cttataggat aatacaaacc agcataaatt tccattgctt
      421 ttagagaatt cgagcccctt ttttgccata tttcctatgc ttttttatca tgtttaaaaa
      481 attacggtta ctcccctttt aaatcttcta attcacacca tctttccgtc aaagcatcta
      541 ctttctcttg cgtttccttt tgatcggcca agagagtttc cacttgttcg taattactgc
      601 cggcggcggc gatagccgcg ttgattcctt tgagcatggc ctcataacga ggcagttctt
      661 ccgtaatttt cttcaactct tcttcttggc gctgcgtcag tccggcctta atctgtaccg
      721 cttttatatt cgttttagta atagaaggtc cagaggcttt ttcacttttc ttttcttggt
      781 gatggctgta tttttcttcc aagtattcac tataatcgcc gtaaaatcgt tcccactgcc
      841 ccgcgtgtaa tgtgaaaagt ttatcaacca cccgatccag gaagtaacga tcgtgagaca
      901 cgacgaggac gacaccttga aaagtatcga gataattttc aaggaccgtc agggtcggaa
      961 tatcgaggtc attcgtcggt tcatctaata agagcacgtt gggctgatcc atgagcaggc
     1021 gcagtaaata aaggcgccgc cgttcaccgc cggacaggac tttgagcagg gtcgacttgc
     1081 cgacgccgtt ggcaccgata atcccgatgc ggtcatgttt aacgacgtga taatcgacgt
     1141 gttggaacaa gggcttgacc gggtcgtaac caaaggccac atcgtcgatg tcgaggatgg
     1201 ttttccccag gcgggctgac gtgtcgagaa actcgacttc accgatgggt ttgccgtttt
     1261 caatagcaca gagctgttca taccgttctt ttcgggcctt ctgcttcgtc gaccgggcct
     1321 gggcaccgcg ctgaatccag gctttttccc gacggatgag gttgcgccgt ttggcttcac
     1381 tggctttatc ccgctccagt cgttcctggc gaagttcgag atactggctg taattgccgg
     1441 catacgtata gacatgccct cggtcgagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccgtg
     1621 tggccttgta cgaactgcgg ttcctggggc agataatgga tgcgcgcctt gggattgaac
     1681 cacaacgtgc cgctgtccat atcggacaga ccggcaatga tcttgaggag ggtcgtcttc
     1741 cccgccccat tgagaccgat gagtcccaat ttctgcccgc tgtcgatggc gaaggtcacg
     1801 tcgtcgaaga ggacgcgaag gccgtatgat ttttttacct gttctactgt cagtacattc
     1861 attataatac atcactttct ctatctggat tttacgttta cactagcaca aagactgcca
     1921 agatgcaagc ctttcggccg gcctttgact atatacaagg cctggcaaat gtgataaaat
     1981 tagttgttat cgtgataaat ttctagagag ataaggggtt attatggcaa acaagtttat
     2041 ctcgggcacc ttgatcctga ccttgtccgg ttttgtcgtc aaagcnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtattct
     2221 cggcggtgaa ggcatcggca tctatcagat ggcctttccg atttacctct tggcactcga
     2281 tatttccagt gccggccttc ccattgcaat ttccattatt acggctgaaa aagtagccaa
     2341 agaggattac ttcggggccc agcgcatctt tcacgtatcc ctgtcgatgc tgtgtacgac
     2401 ggcctttgtc ctgagtgtcc tcatgttctt tggctccagc ttccttatcg atgaacatat
     2461 catccgagaa agccgggctt actactcgct catcgccctg gcaccggcca tcttctttac
     2521 gacgatcatc gccggctatc gcggctatct ccaaggctgg cagcagatga cgccgacggc
     2581 cttatcccaa attgtggaac agttggtccg cgtcgtcgtc atgctgggct ttgccgctct
     2641 cctgttgcct tacggcctcg attatgcagc cggcggcgcc agtctcggtg ccggagccgg
     2701 gggccttgcc gcttggctgg ttctcgtcta ttactattat aaactgaagc ggtcccttcc
     2761 cgtcgacgga cctgttttcg aacaggaaaa aatcaaggat atcttaaagc gcctcgtcgt
     2821 cctggcgatc cctatttccc tgtcgagcat catgctcccc gtcgtctcca acctcgatct
     2881 gctcatcgta ccgcggcggt tagaagctgc cggctatgct acccatcagg caacggccct
     2941 cttcggctac ctgacgggca tgtcggtccc gctcatcaac ttggccacca ttttgacagc
     3001 cgccatggcc atgagcctgg taccggctat cagtcacagc tttaccttga aagagtacgg
     3061 tgaaatccgg gaacggacgg caacgtccat gcgcatcgcc tttttggtca ccatcccctt
     3121 cagtgtcatc ctctacgtca tggcagaacc tatcgtcacc ttcatctaca acgccccggc
     3181 cgcaacagaa gcgacaaggg ccgtggccat cgccatctgt ttcttaggtc tgcaccagat
     3241 ttcaacggcc atccttcaag gcctcaagaa gccgaagatt ccggtcatca acatggccct
     3301 ggcctgtatc gtcaaagttc tctgcaactg gttcttagtc gccatcccgg cctttggcat
     3361 taccggggcc gcctatgcca ccgtcgccga catcggcgtt gcggccgtcc tcaacatgtg
     3421 cttcatctac cgtgagatcg gctacgtcat tgaagtcaag gtcatcttaa agaacatcgt
     3481 ctcggctgcc gttatgggcg tcgccatgta ctttatgtac ccttaccttt tcagccatat
     3541 cggcctcttc ttggccctct tcctatcgtg catcatcgga tcggtcatct acattggcgg
     3601 catggtctta agcggcggtg ttacccgtca agacgctgcc aaaatgccct tcattggacg
     3661 atttatttaa ccacagcaaa a
//
LOCUS       DAWDXZ010000126         3646 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5915_length_3646_cov_10.089390, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000126 DAWDXZ010000000
VERSION     DAWDXZ010000126.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5915_length_3646_cov_10.089390"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1085..1094
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          819 a         1069 c          978 g          770 t
ORIGIN      
        1 cgccgttcag gaagtacagg caaaatatgg cggccgcgta ccggatacga agaaggaagt
       61 acgggctttg aaaggcgttg gtgactatac tgccggcgct attttaagct tagcctacgg
      121 gaagaaggaa gccgccgtcg acggcaacgt cctgcggatt tttgcccgtc tctacgatat
      181 tgaagaaaac gttttgacga cggccgtcaa gaaacaggtg gccgccctgg tcgaagaaca
      241 gatgcccgaa gctcgtccgg gcgccttcaa tgaagccctc atggatttag gcgccacgat
      301 ctgcattccc aagcatcccc gctgtgaaga ctgcccctta ggggacatct gcctggcccg
      361 tgctgcaggg aaggagggcg acctgcccat tcgtcaggtc aagaagaagg cgcctgtcga
      421 aaagattacg gtcgtcgtcc cttgtcacgg gacgaagtgg ctcgtccatc gtcggccgcc
      481 gaagggcctt ctggcttcca tgtgggaatt tcccaacgcc ccgggaacgg gacaggacgg
      541 aatcgacgcc gtccgtcgga tgctggccga aaagggcctg caagtttccg tcgacgccag
      601 tccggccggg accttgaaga gtattttttc gcataaagtc tggcagctga ccgtttatga
      661 aggcgagctg accgacggaa atatcaaaga agaggagaac tggcagtgga ttgaccgcag
      721 cgactacgat gccctgcctt gggccggtcc ccacggtaag atcacagcca tggtataagc
      781 catgaaggga acttttttca ttacgttcat gatttttacg tcctgcgtct gcgtcctggc
      841 gtctttctta tgggactaca ccttccgctc aggccaccgc aaggccctga tcttggggac
      901 cctggggctg acattgttgt ccgttgccgg attcagccac tatgaagagg ccttcccggg
      961 cttgattctg tccggttggg acggactcta tgacggccga agcatggcgg ccctggtttg
     1021 gctcttggcc ttttttctgg ccttgccctg cctcatcgtc gtcgccctgg ctgcggcggt
     1081 ccatnnnnnn nnnnaccggc ccctcagggg atgacccgtc ggaccttcgt caagggcctg
     1141 gccgccctca ttccggcggc ggcctttgcc accagcggtg ccggcaatgt cctcggcgaa
     1201 agcgatttag atacgacgca ccacgtctta aggtataaga atttgccgga ctacctcgac
     1261 ggctataaaa tcggtcaact ctcggacctg catatgggac tcttcttcag ccccgagcgc
     1321 cttcaggaat cgatcgatgc cgtcgccgca cagggcgtca accggctgga aattacgggc
     1381 gatctcatcg atgagatcgt ccttctgccc aaggcccggg atattatcaa tgccaatgcc
     1441 ggccgttttc ctgacggcat cgatttttgc tacggcaatc atgaatatta ccgcaatttt
     1501 tcggccatta cggccatgct cgaagatacg ccggtccgca tcctgcgcaa tgcgtccttt
     1561 gaagccagcc ccggctacgg aaggggccta aaggggagga ggggcagcga cagccgaccc
     1621 ttctacattg ccggtacgga ctacagtttt gcccaagggg aagaagcctt tgccgaacag
     1681 cggcaggact atgtccgcca gaccttggac ggcgtgccgg aagatgcctt cgtcatcatg
     1741 ctggcccacc attcggcctt catcgacgaa gcttttgaac gcaatattcc cctgaccctc
     1801 tgcggtcata cccacggcgc tcagtttgcc cccatcggcc ccttggttac agctgtgggc
     1861 tttaaatacc tgcgcggcat gttccgcaag ggcgaaagcc ggggctacgt caaccgcggt
     1921 accggccact ggctgccttt ccgtgtcctt tgttcccgcg aagccagtgt ctttgaatta
     1981 cgaaaacaag cgtagaggat gatatagatt atgatgatca acgcagcagt gctccatatt
     2041 tttgatttca ataccaacgt ctgcatcgtg tcccagaagg ccctcgactt cagcagcgac
     2101 gtcgtctatg aatacgtcaa taagaggctg agccggatcc tcggcgatgc gtcccagcag
     2161 acgggcgtct tctatgcgac cagtgccttt cagcccaagc tccaagctgt catcgacggc
     2221 agcctgacct ttgacgaact ggcggccgat acggcccgcg aactctacca attggtggct
     2281 cactgcgatg aaccggagtc gacagacctc ttggtcatcg actttcagga tgacgacgac
     2341 gtccgcaaca tggccgtcct catgctggac aataagacgg cctataccca tcagatcctc
     2401 gacgacgaag ggacggtcta caacaagctc atcaagcact acgccatttt gcccggtatg
     2461 gctcagaagg ccgaagccta tgctctcatc cgcctcagtg acttttccat tcacttcgtc
     2521 gacaagaagc gcaagatgga tggcgaagac gtctacctcc tgccggaccg cctgctccag
     2581 tgcacgtcgg tcatttccag taaggaagcc gtcaaggtcg tcagcaagat cgctgaaaaa
     2641 gtggccgaag accacggtgc cagcacggtc gaagccttgt ccaaggccaa gacctattta
     2701 gtggaaaacg ccgaaaatgc cgattccttc tcgccccagg acatggggac ggaggtcttc
     2761 ggcagctcgc cggccatggc tcaggaattt cagcagcaag tggccgaagc taaactgcct
     2821 gacgtcgtgg ccattgaaaa ggaatacgcc cagaaggccg gccgcagcca taagatcaag
     2881 accgatacgg gcattgaaat caccttcccg tccgaatata tcgaaaacac cgattttatc
     2941 cgcttcatca acaatcccga cggcaccttg tccatcgagt tgaagaacat cggaaagatc
     3001 gtcaataaat agttattttg tccgcagccg acagctgcag cgaggtggcc cttatggata
     3061 tgtctcatta caataaagat tacgatacgt cccatccttt ccgcgtcgtc gccccttatg
     3121 aaccgatggg ggaccagccc gaagccattg aatccctggc cgaaggcatc gagtccggcc
     3181 aatgggccca ggtcctcctc ggcgccacgg ggaccggcaa gacctttacc atggccaagc
     3241 tcatcgaaaa ggtccagaag ccgacgcttg tcatcgccca caacaagacc ctggcagccc
     3301 agctgtgcag cgaattcaag gcctttttcc cggataacga agtcggctat ttcgtcagtt
     3361 attacgattt ttatcagcct gaagcctaca tcgcttcgac cgatacgtat atcgaaaagg
     3421 atgcgtccat caacgatgaa atcgataaac tgcgccactc ggcgacgatg agcctctttg
     3481 aacggcggga cgtcatcatc gtcgcttccg tgtcctgcat ctacggcctc ggtgaccccg
     3541 aagactacag cgaactcgtc gtctctttgc gcctgggaca ggaaaagacg caggaagaaa
     3601 tcttgaggaa gctcgtcgac atccagtaca accgcaacga catgaa
//
LOCUS       DAWDXZ010000127         3611 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_5964_length_3611_cov_7.690664, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000127 DAWDXZ010000000
VERSION     DAWDXZ010000127.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5964_length_3611_cov_7.690664"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    277..286
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3076..3085
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          860 a          995 c          903 g          833 t
ORIGIN      
        1 ttcaacatct acctgcccaa gcaggaatgg ctcctcttcg gcgtatcggc tatcatggcc
       61 atcttggtca tcatcgtcag cgtagcctct gcacggaaat gccttgaatt ggcaaagacc
      121 gtaccgccca gctacgatac gtctgaaacg gtagaagctg aagaattgaa acacctcgaa
      181 gaattagtta aatccgatcc gattgccaaa cgctttaaag aattgaccgt cgatcaacac
      241 taagaccata taaaaagact gcccctgtga cagcgcnnnn nnnnnnacat gctctcaggc
      301 agctgccggg acagtctttt ttatatgcat ttttatcgat accgagatcg ttagataacg
      361 ccggttccgt caaaatcggg aatgaagtca tcgctggcgc tctctttttc ctgaacaaat
      421 ccgtcttcaa taccgctgtc aaagagatac tgttccgcct catcgtactc ttcatcggtc
      481 aagggacggt tgatttccgg atagtctccc gcccggccgc aaggcacgta ttggcgcatc
      541 aaactgaata aaacctggcc gtcgtcgaaa tggttccgca cccaatcgat gacggctttc
      601 gtattgtcga cacagccggg aaggatgagg tggcggatga tgacgccttt tttcaaaatt
      661 ccgtcgttat cgagttcgta aggacctacc tggtcataca tgcgaagaat ggcttcggct
      721 gcgacctgcg ggtagtctgc agcaccgctg tagcgttcgg ctaaggctga atcgaggtat
      781 ttaaagtccg gcaaataaat ctgcacctta tctttaaatc gattgatggt ctctaccgat
      841 tcataaccgc cgcagttata gacgacgggc accggcaagg gatctgccaa gctctctaaa
      901 atggcagagg tatatggcgt cggcgtcacc agattgatgt tatgggcgcc cttttcaatg
      961 agctcactat agatttcccg gagccttccg ggcgtaatcg gaactccctt tcgtcccatg
     1021 gagatgatgg aattttggca gaagcagcag cgcagattac agctcgtaaa aaagaccgtt
     1081 cccgacccgc gtgtcccgct gatgcacggt tcctcccatc gatgcagggc cgctctggcc
     1141 acgacgggct ggagcggaga atggcaaaag ccgggcattc ccttctcatt ccaaaggagg
     1201 ctgcggacga caccgcagcg tcgaggacag tcattacata tcgattccat agtcacgcac
     1261 ctcaatcaaa aatctttccg ggatttaaaa tccccttcgg gtcccaagcc cgtttcaaag
     1321 ctttcatcaa ttccaactcg acggggtcag tatattttgc aaataaagct ttccgggcct
     1381 gaccgatacc gtgttcgccg gaaacagcgc cgcccagacg ataggctgct tcacaggcct
     1441 tatcttcaaa agccgcgata cgacctcctt catcggcctc atctgcaaag ccccgacgca
     1501 ggaggtccaa gtgaatattg ccgtctccgg catggctgac accgcggaat acgacgcccg
     1561 tcgcttcacc gatggccttc aactcgttca tgaaggacaa tagtttatcc ggcggtacga
     1621 cgaagtcttc catggcggcg accgggcagt ccgaagccga cgcttcggta aaggccttgc
     1681 gggccttcca aactttttcg gcatcggcca tgaagacttc gacagccccg agagatcggc
     1741 agatgtcatc gagattcatg cagtcatcgt caaggccgtc ttcactttga ctgtcaagct
     1801 ggataatcat ataggctccg ccttcagggc agttctgttt ttcatcgagc cattccccga
     1861 cgacttcgat gacatcgtaa tccataaatt ccaaacacgt cggcgtaata ccggccttgg
     1921 ggagcgccgt caccagcgac aaggcctgtt cggccgatgg aaagacggcc aggacgtgtg
     1981 ccgtcgactt tggcaagggc acaagcttta aggtcagttt cgtaataatg cccaacgtcc
     2041 cttcagaacc aacgacgatt ttttccaagg ggtagccggc cgtcatctta tggaggcgct
     2101 taccgagatt agcaattttc ccggtcggcg tgacgacttc aagagcatag atttggtctc
     2161 tcgtcgtccc atacttcacg gcccggttac cgccggcatt ggtggccccg ttgccgccga
     2221 tgcagcagct gtcgccgctg cacgggtcac cggcatagag aaggcccctt ttaccggctt
     2281 ccttttgtat cgtctcggta atgacgccgg cttctacgac catgtacata gccgcttcat
     2341 taatttccaa aatgcggttc atccgctccg tactgactat aatgccgccg cggccgtcga
     2401 caagggcacc gccttcaagg cccgtcccgg tccctctggg aatgacggga atgacggcct
     2461 cgttagccaa agcgacaaca gcagccaatt cctccgccgt tgccggtaag acgacggcat
     2521 cgggcagaaa aggcaccgca aaggtgcctt catcgcgacc gtagggaatc cgtttatcgt
     2581 catcggtcca aacatatttt tcacctacaa tggcctgtaa ggccgttatg actgcagggg
     2641 ttatctgatt atactccatg tattcatcct cataagacag cccgtcctgt tagaaggacg
     2701 ggcacaacgc aaaacgagca attccagaga acctgattac agaaagaggc cccgcaggaa
     2761 cggcacgagg gtaaactgat agattttcaa gacgccaaag acgcaaaggc cgatggaggc
     2821 gccgacgatg gcaccattga tacgaatcca ggccaggtct tcgccgacct tactttccaa
     2881 gaactggttg aaccgatttt tatcgaagcc ggacaagacg tctcggacag caacggcaat
     2941 gagggaatgt tcatgttcca agatgacgtt gaacgcccgg cggatctgtt catcaagcca
     3001 ttccttgatt tcagggtagg aacggcagta ttcgaggtag tggatgaact gttcttccag
     3061 taaatctttc aattcnnnnn nnnnnccact gctcgatagg cagggcatcg acgaattcat
     3121 tctgccagtt ctgtacccca aaggatagct gaccattcat cgaatagagc aggatttcca
     3181 cttttcggca cagccattcc cgctgttcgc cttcagcatg ttcccattgg tcgagccagt
     3241 tgacgatacc cagttttata tccttggcga tccgctgagt atcgacgata ccgagaacct
     3301 tagtcaaggt aatgctggac atagaggagt gctgcgattc ttcgcgccac tggatgatga
     3361 gccgttccaa cagtttgtca aaatcttcgc tgttgacgac gcggcgaacg agattgatca
     3421 ggtcctggaa catggggctc gtcggatctt cgagcagcga aacgaggcgg tcttccaaga
     3481 agcttaccaa aggctggcgc ttcaaataca tgcggaggtg cccggctatg gccgtctgag
     3541 tttcaccacg ctgaatatgc tgatggaccc gttgagcgac ttcatagagc cccgtacgga
     3601 gtttttcccg c
//
LOCUS       DAWDXZ010000128         3587 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6010_length_3587_cov_7.787373, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000128 DAWDXZ010000000
VERSION     DAWDXZ010000128.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6010_length_3587_cov_7.787373"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1371..1380
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2995..3004
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1000 a          827 c          878 g          862 t
ORIGIN      
        1 agttatctca aagaaatagg ttcacaggca ttgcaggatg tcactgaccg catcgataga
       61 gcgtacaatc tcttctggac aaatctcaac aataaacgta agaccgcacc gccaggtttt
      121 aagaaagtct gcaagtacaa gtcttatacg ctcaagcaag ctggctggaa gcttgatgag
      181 gggcatggca cagtctacat agccaaacag aagtaccgct acttcaagag ccgcaacatc
      241 gaaggcagaa tcaagacact tacggttaag cgggacactt tgggagacat ttatctctac
      301 ttcgtctgtg aacaaaaaat tacagaagtt aaactacgaa caggtaaaag cgtcgggttc
      361 gatttctgct ttaaagataa aatgctcgtt gctccggatg aggaaaatga tattgccgcg
      421 ccatccttct ttcagaagaa ccagaaggct atccgtacag cctgtcgaaa tctgtcgcgc
      481 cgtcaactct gctcgaagaa tcgtcaggag tatcgtaaag tattagcacg gctctatcgc
      541 aaacaagcga atcagcgcag agatttccac tttaaactcg caaaagagct atgtcagaag
      601 tacgcagtta tctgtcttga aagtctcagt atgaaagcta tgcagcaagg tcacgggaaa
      661 aaagtcaacg actacggttt tgctgcgttc gagaatatcc tagaatatgt tggccgtcag
      721 acaggaacac aaattattaa agtagataaa ttttatccat ccagtcagct ttgccatgac
      781 tgtggctacc aaaacaaagc aattaaggat attggcgtct atgaatggac ttgtcctttc
      841 tgcggaagcc atcacgaccg agaccgcaat gcggctagaa atattttgtc agaaggtctg
      901 aggctactaa cagcataaat ttgtatacgg gtgggacatc atccgttagg agagagaacg
      961 taagaccatc gcgagatgcg cagttttcgc tcgtatccta gaatccctcg gctttagccg
     1021 tgggagtatg tcaatcgatt agaatcccct ttcatagcat ccgaggctgc tgctaaatct
     1081 ataacgggat tcatacgatg gccgggttga gtaagggcca tcaagaggag gaatctgtat
     1141 ggaacaacat aaagaacagt cggtagatgc aaagacaaag cccttgaccg gtgtccgtgt
     1201 cctcgatatg acccatgcct acagcggacc cttttgtacc atgcatctgg ccgatcaggg
     1261 ggctgaggtc attaagctgg aagtgccggg aaagggggat cagtcccgtg gctgggcccc
     1321 ttttaaaaat ggcgccagcg ggtattatgc ctatatcaac cgcaataagt nnnnnnnnnn
     1381 ctgaaaacgg aagagggcaa agagattttc aaaaaattaa tcaaagaagt cgatgtcctt
     1441 tgtgaaaact tccgcgtcgg taccctggaa aaattgggtc tcggttatga ggtcttaaaa
     1501 gacatcaatc cgtccttgat ttacgcttcg atttcaggtt ttggtttgga aggtccggaa
     1561 gcggaactcc ccagttatga tctcatcgcc caggctatgg gcgggctcat gagcatgacc
     1621 ggtgaaaagg accatatgta taaagtagga tcgtccattg ccgacaacta ttccgggacc
     1681 tatttgtccc tggctatcgt catggcctta ttccagcggg aacggaccgg catggggcgg
     1741 cgtgtcgatg tttctatgct ggatacgatt ttctctattt tagaaacggg agccctgtcc
     1801 tacgaactga acggagatat tgtagaagcc gtaggcaacc gaaatctgtc cgtggctccc
     1861 tgtgatattt ttaaagcagc cgacgggcaa tttgccctgg tctgcgggac ccagcgcttt
     1921 tggcggtctt tgtgtcagat tatgggaaaa ctggagctgg ccgatgaccc gcgatttgcc
     1981 gataataaac tgcgctgcga acattatccg gacgaattga aaccgattat tgaaggctgg
     2041 agccggcagc acacgctgga tgaactggaa acgaagctct ccgatgccga aatccccttt
     2101 ggccgcattg ccgataccaa gggggtgtgc gaaaacccgg tcataaaacg gcgccatatg
     2161 ctgtgggata ttgacgatcc tgccattggg gagacgattt ccgtgccggg gacgcccatc
     2221 aaaatccatg gctgcgagga tagggccttt cgtccggcac cgaccttggg gcagcatacc
     2281 gacgatatcc tcaaagagat agtccatctg acagaggaag agattgcccg ccttcatgaa
     2341 gaaggagtta tttaagagaa gggacggtct ttagagattt tttcacctca tagcaggaaa
     2401 tcaacctctt tatgtctaat atttatagta taatagatat ataagacgac acacgtgccg
     2461 ttttctttta aaggagtgat actatatggc aaaacgtact gcatccgata ttatggaaaa
     2521 agcttttatc acggcaagcc cggaaacgtc tgtgtttgac ttagtcaatc tgtttgtaaa
     2581 gaatcgcatc accgccattc ctatcatcaa tgacaaggaa gaattggtcg ggatcgtaac
     2641 cgatgccgat ttactgtaca aaaaggtaaa accccacgtt ccgcattacg tcaatttgct
     2701 cggtgccagc atctattaca acggtatcag cgaatacgac aagggcttta agaaactttt
     2761 ggcctgcacg gctcgcgaca tgatgaccaa agacgtcatc attgcagcac ccgatgctga
     2821 tgtcgaacag attgccggcg tcatggtagc cgaacacttg aaggtcattc ctattgttga
     2881 agaaaaacac attgtcggca tcgttacgcg cggcaatatt ctcgatgaat tgtaccgtga
     2941 atacggcgaa gattaaaagg gggagaagaa gatgacggaa gacatgaaga aagannnnnn
     3001 nnnnttgaac cggttgaaac agcagcggaa aaaccggccg tcctcgttcg ggacgtcatg
     3061 gaaaaggtat tcgtatcggt cacgccggat acgcccattc gcgacgtatt tcgccttttt
     3121 atcaaacatc gcctcctggc cgtaccggtc gtcgacgaaa acagccatct catcggcctc
     3181 atttccagtg ccgacctcat gtatcgcagc tcgaagccgc atattcctcg cctgcctttc
     3241 atgggcacca aagtgtatac caaccgcatt tgtaagtacg tcaaggaatt taaaaacctc
     3301 ctcgatcagc cctgtaagaa gctgatgacc aaagacgtca tgatcgccac gcctaaagcc
     3361 gatatcgaac aggtcgccgc cgtcatggtc attgaacact tgaaaatcct gcccgtcgtc
     3421 gacaaccatc gcgtcgtcgg catgattacc cgggcctgca tcttaaagga tttgtatcgg
     3481 gaatggaagt aatcgcctcc tttgtggcta ggcatggacg ctgtcctgtg gtataattat
     3541 atgatcttat catttaggca gctgtgctgc taaaaaagtc attcacg
//
LOCUS       DAWDXZ010000129         3559 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6051_length_3559_cov_6.075342, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000129 DAWDXZ010000000
VERSION     DAWDXZ010000129.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6051_length_3559_cov_6.075342"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2174..2183
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3013..3022
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          874 a          744 c          900 g         1021 t
ORIGIN      
        1 aaacagacga ttcctcggga gggctttata cagcttgcta aggatttcca tattgatttg
       61 aatacggagt tattggatta atcattttta taaagggagg aaaaggctat gttaggtgta
      121 aaagtcgatt acgagatgtc tgcttggaaa aagaccttaa ttgccgggct catcagttac
      181 atcgatgccg gttccatcgt agcaggggct tccggactgt cgatgtggca aggctatctc
      241 ggcatgagca gtgtgcagat gggccttctg gctgcgctgt catcgaatac gggaggggct
      301 gccatcgggg cgctgatcgg gggtaagatc tgtgataagt atggccgtaa attcgtttat
      361 aactacgcac ttctatttta tatgatcggc gtggcgatga tttgcctggc aacgaactat
      421 cccctgttcc tgctcggtta cgtcattgtc gggttaatgg tcggcgccga tgtcgtcgct
      481 tcatggacct tcattgcgga agaagcgccg tcgaaaaatc gtgccaaaca ctgcggttca
      541 gcccaggttg cctggatgct cgggcctgtt gtcgtattcc gctcaattcg atgttcggcc
      601 ttctcggtaa ccgtatcatc tttgcccatt tgatcgtcgt tgccggctgg atttggtatc
      661 agcgtttgaa gatgccggaa tcgacggaat ggacagaagc ccataaacgg gaacaggaac
      721 ttgcggcaaa aggcattaaa gttcgtaagg tccattatgg cgatatcctt cacggtacct
      781 gcctcaagac ggtggtcatg ctctgtgccg tttatacgct ctggaatctg gctgccagta
      841 cgaacggatt cttcatgcct tatgtgtacg aacacctcgg taagctctcg aacgccatgt
      901 ctttgttcct gacggccttg aactttgccg tttgtatctt gacgactttc atctttatgt
      961 acacgggcga taaatttaat cgccgcaagg tcttcggtat tttttccggt ctcggtgtca
     1021 tcagttggtt cgtttgggcc ctgccggcga acatgatgca gatgtggatg ctcttcttct
     1081 tcaccattgt ctggggattc tcgatgcagc agcagttcta tcaactttgg agctccgaac
     1141 tcttcccggt ccgttatcgg gcaacggcac agggattcac cttctttgtc acgcgtttct
     1201 ccgccgctgt ttggggcttc ctcgttccta tcctgatgga aacggtcggt ttccaggtcg
     1261 ccgcgatgat catgatcgga ttcgtattta tcagctgggt tgccggtacc ttctttggtt
     1321 ctgatgcccg cggcctgacc cttgatgaaa tcacgaaaca gcgctatgac cacgaaatcg
     1381 atgaaaatgg ctggtttgtc aaaccggtcg aacataatgt caatgccatt catccggtag
     1441 gaaaatgaca gccgtctgca tacgattgag gtttagcgta taagatgaaa aagagccttc
     1501 gccgctccca aacgattcct tttgtcgggc aattggagaa gggggagacg aaggttcttc
     1561 tttttgatca ttttgatgaa catgtaaggg aggttttttt gatggaacgt tttgcatgga
     1621 aagcgacggt caaagaggga aaactcgatg aatataaata tcgtcatgcc catatttggc
     1681 cggaaatgaa gcaggtactg aaggatgccg gtatttcaaa ctatacgata tggaatgtcg
     1741 gaaatgaact gttcggctat tttgaatgcg aaaaaggggt ggcttatgcc gaagcctatc
     1801 aggccaagag cgctgtcgtt gacaagtgga atgagtacat gaaggatgtc atggtcatgg
     1861 aaatggatcc aacgacgggg gcacagccga agatgcagca ggtattcttc ctgccttgaa
     1921 atcgtttcct aaaaataaaa aacgaatttc ttatggacat gaaatgacct cctaagatta
     1981 gatccgaaca tctaatttag gaggtcgttt tttgtaaatc agcctttgtc agaagattaa
     2041 gttgatctgt ttatctgcca gaaattcctg ccattcctta gaaggcggga catcggtgac
     2101 gagggtgtcg atgcgttcga aatcatcaca tttaacaaac gccgttttgt caaatttcga
     2161 atggtcgacc aatnnnnnnn nnnaatagga tcgtattgcg ggcctgctgc atcatgatgc
     2221 ttttgacgcg gctttcgctt tcgttggatt ccataatgcc cttgttcaag tcgatgctct
     2281 tacagctgat aaaggcaaaa tcgacgtagt atcgggtaag tgtttcacag gctatggaat
     2341 ctgtcagggc acaggagctg tcgcgcatcg tgccgcctgt gcagatcatt ttgaagccgc
     2401 agccgatgaa atcgttcatg agacggatgg aattggtaac gaccgtgata ttacgctgat
     2461 tctgaaggcg ctgcaagagt gcttggcagg tcgtgctgga atccatcatg atcgtgtcgc
     2521 cgtcttggat caggcttgcg gctttgtcgg cgatgagcag tttgctttca ttgtttatgg
     2581 agctgcgacg cgtataagaa agttcatcac tcgtgctttc cgttatcacg gcaactgcgg
     2641 ctgagcaact cctgtctttc cagtttttcc aggtcgcggc ggattgtttc ttcggttact
     2701 ttgaataact tagcaagttc tgagacgtaa actttgcggt caattcgaat cttttccatg
     2761 attttttgtc tgcgttctaa tcccagcatg ataagctcct tctttctctt gttttctgtt
     2821 atctttttct atttcccaat gattcgctct tattataggg caatttgagg atcaatacaa
     2881 tagaaacaga taaaaataga cgtttgattt gttggatgtt gtctctcggt tcacttgaaa
     2941 agaaaggtgt cttcataaaa aatgagcgaa agtaccctga taagaggagc tgtttgacta
     3001 cgcccatgca ggnnnnnnnn nngggtataa aaaaagagga ttttttcgga ggacatagac
     3061 tttggattta ttcgtatggg agggagcaag gtgttttcaa gaagggcagc gtggtagaat
     3121 cgataggaga tcggaagggc ccccctgcga atatattggc agcgacgtta tccgaataga
     3181 aatctccata aagtttttgt gagacgtccg aatccatgat acaatcaaaa atatttgatg
     3241 tttcgtgacc ctacatacac gtgacttatt ctctgtaaag gaggtatggc aatgatagat
     3301 ttacaagaac ggaaaaaaat ctggcagcgc ttcatcgaag agggcatctt gagtaaacaa
     3361 ttgcctccgc ttattgcccg ttcatggcag gattgccgca agaagggaat cgatccgtgg
     3421 caccgaaaaa atcttcccgt tgatttagaa aatctaaata gaagtcgtca aaaaaacaga
     3481 ttattgttgc aagttgccgg acccattatg caaagtgtcc atgaaattgt ccgggaatcc
     3541 cattctcttt tggccttaa
//
LOCUS       DAWDXZ010000130         3551 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6063_length_3551_cov_8.535469, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000130 DAWDXZ010000000
VERSION     DAWDXZ010000130.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6063_length_3551_cov_8.535469"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3184..3225
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          807 a          801 c         1034 g          867 t
ORIGIN      
        1 ctattaatga gaaccttgga tgacgatttt tccgccattc aaggttcttt ttttgaggga
       61 gacagaaacg ctgacattcc tgtgttggcg ggacgtgagc tatgacggtc acgtcttttt
      121 ttgccttcag ggcctcaggg atatcgtgta gaaatcatgt agggctggtg atgattcgaa
      181 ttccttagaa acgtcgggaa ctgtcaggca gcgaaaaaat gaaaagggag cgaccgtggg
      241 aatgagcgct gttagggggg agtagtggtt caaaaatcga ctgtatagat gcgaaaagac
      301 ggcacgaaat gattcgtgcc gtcttttaat atgagtaggc ttttacatgt caaagggatt
      361 ttcgttggga tagagcatgc cttttggctg gttgaaggcg atgtcttcaa tgcccaggta
      421 ctggaagatg gagaagagga cttttccgat gtgggcaaag gcgacggcgc cgtgatgagg
      481 atactgcttg gcgatgagga cgtgacggta gaagcggccc atttcgggga tggcaaagat
      541 gccgatgccg ccgaaggatt gggtagcagc cggaaggact tcaccttggg cgatgtaagc
      601 ccggagctgt ccgccggccg tgctctgaag tcggaagaag gtgatcgggc tttcggcaat
      661 gtccccttcg agggtgccgc gagtgatatc aggtgtgccg ccgtcttcta agagacggtt
      721 ttggatgagc tgatatttta cgccgccctt tttcaacttg cacaaaggtg tgttgccgca
      781 gtggaagccc atgaaggtat ctttctgggt atagttcgtc gtcggtttga tatccgattc
      841 gtagagatcg cgggggacgg agttgttgat gtcgagcaag gtgacggtgt cgccgctggc
      901 acacatgccg atgtattcgc tgacggcgcc gtagatgtcg acttcgcagg cgacagggat
      961 gccgcgggat acgagacggc tgttgacgta gcacggttca aagccgaact gggacgggaa
     1021 ggccggccag catttattgg caaaagctac atactgacga gagcctttgt gagcttcagc
     1081 ccagtcgagg agggtcagtt cgtactgtgc catgcggggc aggaggtccg gccaggggtt
     1141 gccgtctttg ccgagttctt ccgtcatatc ggcgacgacg tcggcaatgc gcgggtcgtc
     1201 tttgtgagcc tgatacgata cgagaaggtc gagttcggag ttttcttcga tttcaatgcc
     1261 taagtcgtag agcggtttga tcggtgcgtt gcaggcaaag aagtcctgag gacgcgggcc
     1321 gaaggtaatg atttttaact ggctgaggcc gatgagcgtg cgggccatgg gctggaagtc
     1381 ggcaatcatg gaagcgactt cttcagcggt tcctacgggg tatgacggga tataggcttt
     1441 taatttgcga aggcctaagt tatacgaaca gttgagcatg ccgcagtaag catcgccgcg
     1501 gccattgatg aggtttttgc ctgtttcttc ggcagcagcg gcgtacatga ccggaccgtc
     1561 gaagtattta gcgattaagg tttccggcgt ttcgggaccg aagttaccta agaagacgac
     1621 gagggcattg cagccggcgg ctttagcttc ttcaacggct ttgagcatat ctttttcgtt
     1681 ttctacgacc gtcggcactt cgtagaggtc gccatatttt ttcgtatagg catcgacgat
     1741 ggccttgcga cggttctggg ataaagaaat aataaagcag tcacggctga cagaaataat
     1801 accacatttc acttcgggaa tattcataat aaggtctcct ttcccatgta agggttactt
     1861 gtaatttttt tgagtgccta ggcacatttc gttaagggaa tgatagcacg cttgtcaaat
     1921 ttctgtcaat acatagtttg acattttttt acgaaaggca gtattatgca gaaaaaatac
     1981 aggtaaaccc tgaaatatag caaaagtgca tgtatgaaga ttcccgttga ccatctaatt
     2041 ttgtcatggt atacttcaaa cgtagacatg tgcttaggca cgcaataagg cgcaaggacc
     2101 tcgaaataaa tgaggcacat tagggaggaa gatatatgaa gtatgtaatg ggaattgata
     2161 ttggtacgtc agcgaccaaa acagtactct tcgacgatac ctgtcaggct gtggctatgg
     2221 cgtcctgtga gtatcccttg tatcagcccc agaacggttg ggctgaacag aagccggaag
     2281 attggtatca ggctgttgta cagacgatac gggaaatctt ggcaaagtgc accgtagaag
     2341 cgaaagatat cgtcagtatc ggcttatccg gtcagatgca cggcctggtc atgttaaatg
     2401 aaaagaacga agtcattcgg ccggctatca tctggtgtga ccaacggacg gggaaagaat
     2461 gcatcgaaat cacccggacc gtcggtgctg aaaagttgat ggatattacg gccaatccgg
     2521 cattgaccgg ttttacggcg tcgaagcttc tctgggtgcg caaccatgaa gcggaacact
     2581 atcgccagtg ccgccatatt ctgcttccga aagattatgt ccgcctgcgc atgaccggtc
     2641 agtatgcgac cgacgtttct gatgccagcg gtatgcagtt ccttgacgta cgtcaccgct
     2701 gttggtccga tgaaatcctg gatctcctcg acatcgatcc ggcatggctg gctaaggtct
     2761 atgaatcacc ggatgtgacg ggaacactga caccggcctt tgccgcagaa acaggcctcc
     2821 ttccgcaaac catcgtcgtc ggcggtgccg gcgataatgc cgctgctgct gtcggaaccg
     2881 gcatcgtcag tgacggaaag gcttttacaa ccatcggtac cagcggcgtc gtcttcgccc
     2941 atacgagcga tctccacatc gaccctaaag gccgcgtcca ctcgttctgc tgtgccgtgc
     3001 ccgggtgttg gcacgtcatg ggtgtaaccc aggctgcggg cttgtcactt cgctggttcc
     3061 gtgatacgct gggacatgac tacttaaaag aagccgaaca ggccggtctt gatccttatg
     3121 tcgtcatgga ccatcacgtt gaacacgtac ccatgggcag ccggcgcctc ctttatttac
     3181 cttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgacc cggattgccg
     3241 cggtgtattc ttcggactat cggccattca tacgcaggac gatatgctcc gggccgtcat
     3301 ggaaggcgtt ggctattctc tgacggactg ccaggatatc cttcgcgaca tgggaatcgt
     3361 cgttcaggaa atgatggcct gcggcggcgg cggacgaagc caattgtggc gtcagatgct
     3421 ggccgatatg tataattgcg aagtcaaaac cgtatcttct aaggaaggcc ctgccctggg
     3481 ggcagctatc ctggccggcg tcggagccgg attgtttaag agcgttgtca cggcttgccg
     3541 cgatttcatc g
//
LOCUS       DAWDXZ010000131         3551 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6064_length_3551_cov_8.209954, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000131 DAWDXZ010000000
VERSION     DAWDXZ010000131.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6064_length_3551_cov_8.209954"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1279..1288
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          860 a          968 c          918 g          795 t
ORIGIN      
        1 gcgccgtggc accgagctgt gtccggtcgc cgctttccaa gatttcccga aaccgcaccg
       61 gcgtaattcg atggagtccc aaatcgacga gaccgccggc aatgttgcgc accatatgat
      121 ataaaaaacc gtcgccgacg acatcgatat cgaggagatg tccccgctca tgaaaggtga
      181 tggaatagag ggtctttacc ggattcatgg gcgtcgtatt ctgcccttca aaggccgaaa
      241 aatcatgggt gcccaaaagg accgctgctc cttccttcat ggcctccctg tcgaggggct
      301 tgcgaatgaa ccaggcgtag cgcaggccaa agggatcgtg catccgattg ttgtcgatgg
      361 tataacgata atgcttgccg acgttgttat gacggacact gaagtgttcc ggcatttctt
      421 tcgccgagag gacggcaatt tccggcggca aatagccttt catggcatag ggaatctttt
      481 cggtcggaat cgaaccttcc ccgtaaaaat tgacgacctg accgcgggca tggacgccgg
      541 catctgtccg agccgccccg taaatcgtaa tcggcgactg gaagatggcc gacagtccct
      601 gttcgataca ggcctggatc gaagcggcat tggtctggcg ctgaaagccg tggaaatccg
      661 tgccgtcata ggcaacgacg aggcaaatat tcttcttggc cattacagcc accgcatggc
      721 cgccaggatg gccgtcacga tcagcacggc cgccatggcg atggcatcac gataggtgca
      781 ggacaattca ttcatccgcg tccgcccttc cccgccgcga tagcagcgag cttccatggc
      841 tgtcgccagg tcatcggccc gccgaaaggc actgacaaag agaggtacca agagaggaat
      901 catgctcttg ccgcgctgaa tgaggctgcc gctggtaaag ttagctcctc tcgccgtctg
      961 ggctttcatg atgcggtccg tttcttccaa cagggtcgga ataaagcgca gagcaatggt
     1021 catcatcata gccagttcat gcgccggcac accgattcgc ttaaagggat tcaagagatg
     1081 ttcgatgcca tcggtcaata cgatgggcga cgtcgtatac gtcaacaggg atgaaaagac
     1141 gatgaggaag actaaacggg ccgtcatcaa agcgccctga tacagccctt ctttggtaat
     1201 gtgcagtaca ctgtactccc aaaggacttc gccgggcgtc gcaaagacgt ggacggccat
     1261 ggtaaagata atgatgacnn nnnnnnnnct ttaaggattt aaaaataaac cgaacaggca
     1321 gacgggaaat ggcaaaattc aaggccacaa aggctgtgac aataccgtag gactccaagg
     1381 aattggccaa aaaaatggcg acgatgaaaa tcataacggc gattattttt acccgcgggt
     1441 cgagtcggtg caggaaagac gtccccggga aatactgacc cagtgtaata tcagtgagca
     1501 tgacccgtcc ctcccttcag gcagtcatag atagccgcgg cgccttgttc taccgtccgt
     1561 acccggtcgt caacggcaag gccccggcct ttcagatatt gaagcacacc gttcaccggc
     1621 ggaacggcta cgccggcttt ttgcagttca tcaccgtgct gcgcaaaaat cgacaagggg
     1681 tcgccgtcaa ggacgatact tccttgagag aggaccaaaa ggcggctggc catctgggag
     1741 atatcttcca tattatggga caccaagatg acggtaacgc ctttttcctt atgccagcgc
     1801 tggatttcag aaaatatttc ccgccgcccg acaggatcca gaccggcaga cggttcatcg
     1861 agaataaggt aagccggatg aatggcgatg acgccggcaa tggcaacacg ccgcatctga
     1921 ccgccgctta accgaaaagg cgagcgattg gcataggtat cgtagtcaag gccgacaaaa
     1981 tccatggctt ccttgacccg gtcctccact tctgtctcgc taaggccgaa atttcggggc
     2041 ccgaaggcga tgtcgcgagc aatcgtttct tcaaagagct gatattcggg atactggaag
     2101 accataccca ctttatggcg taccgccagg gcttctttcg tattcttccc gatatcggta
     2161 ccgtcgacgg ttacggaacc gttcgtgggc tttatgaggc cgtttaaaat ttgaaccagc
     2221 gtcgacttgc ccgatccggt atggccgatg ataccgatga attcaccttc atgaatctct
     2281 acattcgtat cgctgagggc cgtcttttca aaaggcgttc ccaagccata tgtatgggtc
     2341 acgttcgtta acttaattga cataatgcgt ctcccaattc cttttccgtt ataatcgatg
     2401 ccggaagggg tatccctttt tggcgcagcc gttccgcaag ttcagcggca acaggcacat
     2461 caaggcccat atctttcagt tccttgacct ttgaaaagac gtcccgcggc gcgccatcca
     2521 tcttgacttc cgccttgtcg atgacgacga cccgttccgc ttggacggct tcttccataa
     2581 agtgtgtaat gatgatgacc gtaatacctt cacggttcaa ctgatggagg gtatccatga
     2641 cttccttacg gcctacggga tcgagcatag ccgtcggctc atcgaggacg atacagtcac
     2701 actgcatagc gaggatgccg gcaatggcaa tgcgctgttt ttggccgccg ctcaagaggt
     2761 gcgggccgtg tttagcataa atcgtcatct tgaccctttt taaggcatcg ctgacacggg
     2821 actttatctc atcggacgga atgcccaggt tttccggccc gaaggctaca tcttcttcga
     2881 cgacagccgc tacgatttgg ttgtccggat tttggaaaac catgccgacg tgctggcgta
     2941 tcttccatat attctcagcc tcgtcagtcc gcataccaca gacttcacac gtgcctgacg
     3001 tcggcgtcag caggacgtta aagtgcttgg caagggtcga cttacctgag ccgttggtgc
     3061 cgataatggc gacgaactcc cccttatcga cggtcagcga aatgtccttt aaagcataga
     3121 cgaccttgcc gtcctcatct tcataggcat ggcacaggcg gtctgtcttt atcataggta
     3181 ttgtcatact acgtctcctc acgtaagaaa aaaggctata ttcttgaata ttatacagtc
     3241 aatagcagtc cctgtccata gatacacgca aaaaggccgc gcctcatgac gcacggcctt
     3301 ttcaaatcag cttataaaca agggatcata cagattatta ccgcgcccgt tcgggaccat
     3361 aggcattgtc accgtcagtc cccttcaccg tcgccagccc taagtagcaa acggccatga
     3421 tcgactgtgc cgccaaagct gccgtatcca aagcaaggac cggcagtata taacttgcca
     3481 aataagccat atacggaaca gcaaacaagg cccccggttt attgaggtcg tggaagcgcc
     3541 gggtaccgag g
//
LOCUS       DAWDXZ010000132         3538 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6081_length_3538_cov_7.820844, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000132 DAWDXZ010000000
VERSION     DAWDXZ010000132.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6081_length_3538_cov_7.820844"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1577..1586
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3102..3111
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          823 a          796 c          976 g          923 t
ORIGIN      
        1 atgttgagga ctcgggggat gccgatcgtg ccgcggggag cgtcggtaag gggtttatag
       61 ccgaagagga ggttgtattt tatcttatac atgttcggca ggggcttctt gcgggccgct
      121 gtcgagccgg cagcacctcg ttcacagcgg ttgcccgtaa tgaaggaacg gccgtcgttg
      181 aaggtgttga ccgtgagcag gcagttgttg gtgcagaggc cgcagtggcg gatcgatgtc
      241 gtgatgttta attcctgcag ggctttttcg tcaacgatgg tcgatacgac gtggtcctga
      301 tgataggctg tgcggcagat gattcccatg ccgaaggcac ccatgagtcc tgaaatcgac
      361 ggacggatga cttggcggcc caggagcttt tcaaaagccc ggaggacagc gtcgttataa
      421 aaagtaccgc cttggacgac gatgtgcttg ccaagttttg cagggtcttt gactttgata
      481 actttataaa gggcattttt tatgaccgag taagacaggc cggcagaaat atcggctaag
      541 gtagcgcctt ctttttgcgc ttgtttgact ttggaattca tgaataccgt gcagcgggaa
      601 ccgaggtcga tgggactctt ggccaggagg gctgcctgag aaaattcctg aatggacatc
      661 ttcagcgatt tggcaaaggt gtcgaggaag gagccgcagc ccgacgagca ggcttcgttg
      721 agcagaatgt cttcgatgtg gccgtctttg atgcggctgc acttcatatc ctgtccgccg
      781 atgtcgagga gaaaatcgac gtccgggcag aaatgggatg cggcctgata gtgggcaatc
      841 gtttcgactt cgccaagatc gaggtgcagc gcttctttga gcaaggcttc gccgtagccg
      901 gtaatgccgg ccttggcgat gaaggcgccc ttcggaaggc gggagtagac gtcggcgaca
      961 atctttttag cagctgccaa aggacttcct tcattattct gatagtgaga atagaggatt
     1021 ttcatgtctt cgctcaagac gacggctttg atggtggtcg aaccggcgtc gatgccgaga
     1081 tagcaggggc cgctgtagct gtccatatcg ccgtgaggaa cggtatgctg acgatggcgt
     1141 tcttggaatt cgtcgagttc agcttggctg tcaaagaggg gggcgacccg gtctgacttg
     1201 gccagtgtcg attcatcgac gttcttaagc tgccccatga gattcatgaa ggagaccggc
     1261 ttggtatcca tactccccag ggcggcaccg atggcgacgt agacttgggc gttttctggg
     1321 gcaatgacct tatccggtgt caagtgcaat gtcttggcga attgttcgcg aagctgcgac
     1381 aagtaggtca agggaccacc gaggaagcag accgtgccct tgatgggacg gccgcaggca
     1441 aggccggtga tggtctggaa aacgatggac tgaaagaccg aagccgcgac attttctttg
     1501 ctggcgcctt catttaagag ggcttggata tcggtcttgg caaagacgcc gcagcgggct
     1561 gcaatgggat agattgnnnn nnnnnnatcg gacttcttgg ccagttcatt caagccgctg
     1621 gcttcagtgt cgaggagggt tgccatctga tcgatgaaag cccccgtccc gccggcgcag
     1681 ttgccgttca tacggtgctc attgccgccg gtaaggtagg tgattttggc atcttcacca
     1741 cccagttcga tgatgacatc ggactgagga tagtatgttc gaacggcttt ggtgccggca
     1801 atgacttcct gcagaaaagg gatgtgcagg tagtcggcca gggcaatgcc gccggacccg
     1861 gtgatagcca tggtgatctg acggtcgcca aagatttcat aggcgttttg gagtaaatcc
     1921 caaacttttt ggcgtatatc cgtatagtgg cggacatagc atgcgtgcag gcattgtttg
     1981 gcatggttga gaacagcgat ttttactgtc gtcgaaccga tgtcaatgcc gatgtgtaag
     2041 agattcttca tgttttgtca ccctatcggt ttataaaccg actctttctt taaaaatttt
     2101 atttattata aagactatat ataaaaagtc tactttatat tgtaccataa tccctttatt
     2161 ctgtaagggg gtgatacaaa ggattttact tattaataag aaatcgatat cattttggaa
     2221 accatgaaat gcgctgcgac aaatgagcct aaatatgcta aaatgaagtc actatcagaa
     2281 tatgataaac tcaggatatt atcaaggagt acaatatgaa aaaatttata acggaacatc
     2341 agtcccctta cctggatctg acgacggaag cctcagaaat cctttatcgc ggtcattccg
     2401 actttcagga catcgtcgtc gccgattcga aggaatttgg ccgcatgctg gtcttagacg
     2461 gcgtattcca gacgtctgtt aaagatgaat tcatgtatca tgaaagtatc gtgcacattc
     2521 ccttgtttct tcaccccaat ccgaaacggg tcctcatcat tggcggtggt gacggcggat
     2581 cagccaggga agcggtccgc caccctgaag tcgaatcggt ggctatggtc gatatagacg
     2641 gcaaggtcat cgaattgtct aaacagtatt ttcctgaaat ttccaaggcc atgctggaag
     2701 aaaacccaaa actgaccgta actgtcggcg acggcattgc cttcatgcgg caagccgaaa
     2761 attactatga cgtcatcatc gtcgactgct ctgatcctgt cggccccgga gaaggccttt
     2821 tcacctacga tttttataag gataccttta aagctttgaa ggacgacggg atttttgttc
     2881 agcagacgga atcgcctttc atgcacggca aattggtgaa agatgtattc gactgcgtat
     2941 ctgatatttt ccctgtcgcc cgtttatata cggccttcat tcccctctat ccgtcgggga
     3001 tgcactgctt tacgctgggg tctaaaaaat acgatcccct gcagtggacg ccgaatcgaa
     3061 agcgtacctt cccgacgcgt tattacaatg aagggattca gnnnnnnnnn ngattcagca
     3121 gagcgccttt gtcctgccga atttcgtaaa ggaccttctg tatggaaagg ctgaccgtta
     3181 atcggcttgg tttatagcta tgcatttcag gtctgtattc ttttgtgatt aaatgtaaaa
     3241 aatacttgac tttgtctttg actcatggta agattatcta cgttgacgac gctgattcag
     3301 cgccgttacg gcagataatc ttatatggcg ggtatggtga agtggttaac acggcggatt
     3361 gtggctccgt tatgctaggg ttcgaatccc tatactcgcc ccataggggt atagccaagt
     3421 ggtaaggcac cggactttga ctccgctatg cgctggttcg aatccagcta cccctgccag
     3481 ccatgattca ttagctcagc tggtagagca cctgactttt aatcagggtg tcccgagt
//
LOCUS       DAWDXZ010000133         3492 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6132_length_3492_cov_6.998545, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000133 DAWDXZ010000000
VERSION     DAWDXZ010000133.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6132_length_3492_cov_6.998545"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1028 a          701 c          872 g          891 t
ORIGIN      
        1 aagcgaaaat aataacggaa tggaaaggag cttttttatg aacaagattt atcgcgtcat
       61 ttggagtcgg gccaaacatt gctacgtcgt cgtatcggaa atggccaaaa gtcggactaa
      121 aagtaacggc agcggaagcc gtggtatcag aaggacggcc ttgacggctt tggttttggc
      181 gggattgagc ctgtccttag gatcccagtc gttggcagcg gatccaattg tcattaattc
      241 ggataatgga accgtaaata cagtctcagg agccgataac tctacgataa ttggtgatga
      301 aaataccgta actaacagta agagccaagt tgttattggc gataaaaata ttgttcagga
      361 tagaaatcag ggaactgtat ccggtaaaca agaaaccaga actgataccg taatggatat
      421 acttatcggt aagggaaata aaataactgg caatgatacg tatatgaccg gtaatgaatc
      481 attgactatc attggaaata ataatagatt aggaaatcct acttcggcca ttattattgg
      541 ggacaatcaa tatatttttg ggagtaaatc tatcgttatt ggttcgcttg ctcaaccata
      601 taccggtgaa gcctccgctg gtgctaatgt tattcttgga tatgatacat tatcagaagg
      661 tggcggtagt gtattgttag ggaatagttc ttatgttggt ggcatgttcc aaacagttag
      721 tggatttaat tccattatcg aaggtggtaa tcagcgtatg tcagctgagt ttgccacaat
      781 atatggcgca tttaaccgat caagatatgg tttcgatgac agcactgata attcaggaat
      841 tgatggtacc gctaatagtg ttgtaggtac ttttaatgat actaaaaatg cgaggaaccc
      901 ttatcttagg ctcggggaat gatgttacga attctgtggg ggcaggggat gttggtaatt
      961 taagcaatat cgcgtttggg ttagccatgg actctacaaa tatgtctgcc ggtaaagctc
     1021 ttgactatga taccgttcga aatacggccc ttgatgcact taaatatact ggtggctcta
     1081 cggtagtatt cgggaatgcg aatcaatcgg attattctaa tctatctcaa attataggga
     1141 ccggtaatat tataaaaggg agttctgaca gcattagtgc ctataatatg atgagtggct
     1201 atgaaaatac tggtacaaat attaaacgca ctgctattgt aggtactgga aatattgtaa
     1261 cgaatagtga ggataatgtt atcttaggtg attatcatca aatgagcggt ggtaatcaca
     1321 atgtaattct aggctctcgt gattatacag aaacgaaaga aactataacg tactcttcgc
     1381 cgtattatcc attgtttgat gccgaatatg aggtaacaaa acgcacaccg acttccaaac
     1441 atacggataa catcgaaaac gccgtcatgc tcggctacaa tacagatgta acccaaagcg
     1501 gcggtgtagc cttaggggct gaagcggtag ccagtacggc agccggtaaa gccggttatg
     1561 atccggcgac taaagatgct tcgacggata caaccggtac ttggaaatcg accttagctg
     1621 ctgtttctgt tggggacagc accgatagtg ctaatgttaa aactcgtcaa attaccaatg
     1681 ttgcggccgg tagcgccgat acggatgccg tcaacgtagc ccagctgaag aaagttcaga
     1741 cacagcttga ttctaattcc gttcattatt tctctaccaa gagttctcaa acttctaccg
     1801 gcacgaatta cgcaaacgat ggggcaaccg gtacagacag tatcgttatc ggtatttctt
     1861 ctaagtcgag cgggaataac agtactgttt taggcaataa tgtcagtgtc gtaggggaca
     1921 aaaataattt taatagcagc atcgtcgtcg gtcaaaatat cgatgtcgac ggtacgcata
     1981 atgccgtgtt tgctacggat tacgaaaatg gcgattataa aacgactaaa gtatttggcg
     2041 agcagaatac cgttctcggc gtcggtaact tggtcggcta tacggctgtg caaaatggaa
     2101 atagctggac ctatacaaag aatgattacg gctccgatca gaacgttgcc gtagggttat
     2161 tgaataccgt aagtggcggc agtattgctg tcggtacaag ttctcatgta aataccttag
     2221 gtacatcctt tggtcatggg aactccattg aaggcagtat gctggaaggc ggtcaattgc
     2281 gcattgcttt aggtaactat ttgacggtaa aaggtgaaat gggcttagcg gtgggtaact
     2341 ctgccaaggt tgatggcgat tgggccattg cgattggtaa taataatcgt gatgaaacaa
     2401 cgacaatggc tcaaggtgca tatgccattg cgcttggtac taatgcgaaa gctacgaata
     2461 cttcggcact cgcattggga accagtagtg tggtaagcgg attttatgct actgccttag
     2521 gtactgcagc caaagccaca gtcacccaag gggttgcttt aggttccttt agcacggctg
     2581 atagagtcgg tggtattttt ggctatgatc cgttgactaa aaaagaatcc accagcaaca
     2641 cggctgcttg gaaatccgtt ctcggtgccg tatctgtcgg tggacaagtt aattatagtg
     2701 atggaacaac cgggcaagcg actcgccaga ttaccaatgt tgctgccggc agtgctgaca
     2761 cggatgcggt caacgtggcc cagctgaaac aggttcaaca gtctatttca gatgctgtga
     2821 aggaaggtaa gacaactatt gaagcgggtg ataatattac cgttgaaaaa ggctctacgg
     2881 aaaacagcta caagattacc gcgaaggata cgacacttaa ggactccgac agtgctctca
     2941 gcctcaacgg caataagttg aaactggaaa tcgaagatac gagcggcaat aaggtgtcgg
     3001 gcagcgtcga gttggataaa atcggatcgg ccattgatac caagaacacc atttccaagg
     3061 gcaaacacat cgactacacg gaaaaaacca atgactacgg cggcacggaa tacaccctcg
     3121 acgtcgtgac cgacggcaag gtagaatcgg gcaatacggg catcgtcacc ggcgacacgg
     3181 tgtaccagga aacccgcgtg gctaaagacg gtgaatacgt aaaacaggct aattctgccg
     3241 ctgaaaactt atcggtcttg gatagtcagg tgaaaaccaa cacggaatct atcaccaaca
     3301 tttcaaataa cgtgaccaac ttgaacagtc gggtcagcaa cctcgatacg cgcatcaacc
     3361 gcgtcggtgc cggttccgca gccttagcgg cccttcatcc ccaggactat gatcccagtg
     3421 cgaagtggga ctttgcagcc ggttacggca attacaaatc ggccaatgcc gtcgctatcg
     3481 gcgcctttta tc
//
LOCUS       DAWDXZ010000134         3491 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6135_length_3491_cov_9.781432, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000134 DAWDXZ010000000
VERSION     DAWDXZ010000134.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6135_length_3491_cov_9.781432"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          798 a          837 c          784 g         1072 t
ORIGIN      
        1 tagaaagcaa tggcgtagct gtccttttgt gcagaccgaa gcttctttgc cgattggttt
       61 ggaacgtagt ttaaatcttg tgcaattttg tatattcgct gttgagtttc ctgagaaatt
      121 tttcgttccc gtccttttcc gttcaggacg atggatacgg tagcaataga aacgccggca
      181 gcttttgcaa tatctttaat tgtggacacg atggccacct ccactcttat gcttattttg
      241 acataattta aacgtttttt caaggaaata tatgctacag aatggataca atataagcta
      301 agtgcataat gcaagcaaaa tagcgccgct atcacgtgct agtgatttac ggcgctaacg
      361 atatctcgca gaatctatct atttctaggg cggtggctac atagtggctc tatgttgagc
      421 gtcaaattta accggtaatt tatccaccgc gtcaaccaat tgccgcgcat ctttatgcgt
      481 gtagacgtcg ctggtgacat ccctctgcgc atggcctacg attagcttaa gcgtgaatag
      541 gtccattcca taattccggg ccatcgatat aaatgtgtgc cgggtatcat ggcgtaggtg
      601 ttcccggatt cccagctttt tacagagttg ttttaggggt cgatccaggc ggacctggat
      661 atattttggt ggcatccatg tcggggattg gcagaaacgg gcctgggagt acatctccgt
      721 cacaaacggc atgatgcact tcgcgagggg tattattcta tcgcgcccgg cttcggtttt
      781 tatcccgcca ataacatagc gctccttgat atgcacgtta tcaattttaa cctggtatag
      841 ctctgccggc ctcatgccgg tgtagatgta tatcagcatt acacggacca gcctaatgtc
      901 tgcatgctgc caaaggacct caatttcttg ctccgaaaat ggaacatgca gcgtcgattt
      961 cacggcaggc ggcaaagtaa tcagctctgc gtaattcttc tggatgacgt cgttttttat
     1021 ggccgcctta aacgccccat taatcgcagt taacactttt tcatgcgatc cccggccgag
     1081 atttttgaag gaatcgaaaa tcgcctgcaa atgtgccaac cgtatctgct ggatcggcat
     1141 gctccatatg ggcgcacact ttttcttggc cgttgcatag ccgccctttt tcaagtcgac
     1201 gccttttctt tttttgtccg tgatcatcca gtcccaggcc tgggcgaacg tcacatcgcg
     1261 attgtcgaag cgttccggct caagctgata tgctgccaag gcctggtagg cttccgctga
     1321 tttttcaaag taaccgatgc actttctgat ccgcgtgccg tcgtccttcc agccgaccgt
     1381 aatgactgcc ttaaatggtt ttcttaattt ctgtttgagc ttatacacgg acccggtgcc
     1441 gttagctctt ttcataccca taaaaataag cctcactttc taggtagcgt agaggctgat
     1501 gtggtataat gttaagtata atcagcctct tgttcggtgt gattatattg tcggcagtat
     1561 tggtagtact gtcagcgcat cccgttttcc tgttgccgca gggaagcggg attttttttt
     1621 attgcgtaaa aaaattagct attgatccag gcgtggactt tgtccactgc ggccttagct
     1681 tcggccgccg atggggcgcc ggagtaactg cccacgaagt catcatacgt gccctggggg
     1741 ttctgttgga tgtacttcgt gaataagacg taaaccttat aatcgtcttc cgtgggctta
     1801 tttgcccagt ttttctgcaa tctaaaattt ttcgtgattt tgtcattgac acgttgcata
     1861 cttccgtagg tgtacgggat tcctgcaacg tcagtgtcgg agaaactgac gaaaacgttt
     1921 ttcgttttgg ccgcgtcaat ctgctcttgg ccgatttttt ccaattgctc ctcggttact
     1981 ggagcatcct ggacgacgac cagataggcc gttttctggc tcttgctgtc cgtcgtaaat
     2041 accttgtgct gcggcgtagc aacgctactc tgcgctgttc cagaatccga gccgcatcca
     2101 gcgagggaag cggcggccag tgccgcgctc aggacagcgg ctaaaagttt tgcttttttc
     2161 attttatgca tctccttttc atacaatttt ccgctgatac tgcgatcgcc ctggcaagga
     2221 tagccgataa gccatgaatg ccggcgtaac ctcgaaatat tcggctaacg ccggaatcgt
     2281 tgtaatgtct tcccgataag ccgttacaag ctcttcttga gggataagat actcagctgc
     2341 ccatcggagc gctttagcct catccttgga aaaattacat ctgtttaggc ggttccagtg
     2401 ttcccgcggt agattattcc cggcaagcgt atagtggtgg cctaattctt ccgccaatac
     2461 cacattgcgc aatgggtaat catctctaat agacgtagcc agcataatag tatgcaatcc
     2521 gtcacatgag atatacaagc cgtttaatgg cggcctgaaa tcctcgtatg ataatattat
     2581 gtgctccgca tcacagatgc ggagcatttc ttcatatttc atgcacatca cttctcgttt
     2641 ttcttctgca ccttatttat ttccgtaacc aacttatcaa gggcttttcg tatattttct
     2701 agtcccgctt cagtgttttc gtccaacccc gaagccgcaa tgtctaactg tttattatat
     2761 ttaggatgtc ggagcatccc tttcatttct ccgcggatta ctccgcgatc cgcatcatcg
     2821 agggacttgt acaggtaaat tgcctcagcc aagtcgggcg gggttgaaag atccggtgag
     2881 gttttcggtg catcgttgta acctaaatcc ggccagccca ttaagacgtt aggcggaatg
     2941 cctaatactt tagataactt taccatagca cctctcctca tattggagat ttccccagac
     3001 tcccatctgg agacagtgcc ttcgctgaca cccaccgcta cagataactc ccgcattgtc
     3061 atgttaagtt cgattcgacg atccctgatt atgtctttga ccggttttcc cataaactta
     3121 gtaattacat ctaaatctaa cggttttatt tcgcatgtgg tcatcccgcc tgatgagtca
     3181 gttatagtta tggtcgggct tttttcttct actttttctg atttcttttg attcacggta
     3241 tcacctcttt atctatattc tatcatcatc cttgcgtttt tgcaacaaaa atttcaaaaa
     3301 aagcagaaat atttcattaa cgcattgaca ttatgcgggc ttacgtgtta ttatgttctt
     3361 gcgtaaggaa ttgcgataaa ggaaagataa ggaggtgaga caatgtttaa ccgggcgtta
     3421 tttcaatatg cggtggaccg caaaaacatg actatgcttt cagtcgcaga ccgactggga
     3481 gtaaatcctg c
//
LOCUS       DAWDXZ010000135         3489 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6139_length_3489_cov_7.614444, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000135 DAWDXZ010000000
VERSION     DAWDXZ010000135.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6139_length_3489_cov_7.614444"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          684 a         1054 c          975 g          776 t
ORIGIN      
        1 gccgggcagc aagaatacga cggaagactt attgttcctg aaagaacacg gctgggatca
       61 ggcgcttgtg gccttcatgg agcagggaaa accggtcgtc ggcatctgcg gcggctacca
      121 gatgatgggc gatgccgtcg tcgatcccga cgggagcgaa tccgacttag gatccgtcga
      181 aggccttcac atccttcagg ggacgacgac tttcagcccg gagaagctga cctttcaggt
      241 gacggccgaa tgccggagcc tgcccttttt gggcgagtct atcgacgccg ggacgcttac
      301 gggctacgaa atccatatgg gacggaccga cgttgatggc agcgatgccc atccctttat
      361 cgtaaccagc cgctcgaaag cgccctgcca agtaacggtc ggtgcggccc gggctgatgg
      421 cctggcctgg ggaacttaca ttcacggcat ctttgacaac gatgacttcc ggtgccggtt
      481 cctgaacgtc ctgcgccgcc gcaaaggctg gcaggatctt cccgtttccc tgcactaccg
      541 acaggaaaaa gaagaaaagt acgatcgctt ggctgccgtc gtgagagaat ccctcgacat
      601 gccacgcctg cagcgcatca tagaggaggg gatacgatga ttacggtcat cacggcattc
      661 attatcgatt ttatcatcgg cgacccgcgg acgacgcttc atcccgtcgc cttgatgggg
      721 tcctttatca gcatgctcga aagactcttt tacaatgaaa aatcatcgaa gtgggagcat
      781 ttcatcttag gcggtctctt ggttttcatc accttgttat ttttctataa tctgaccctg
      841 gccatcgtcc tgatcttgga atttcttcct caccaagggg tgaattacgc catccagggc
      901 cttatcttgt cctttatgat ctgccctcac agcctggcca aagccggccg ggatatatat
      961 aattatctcc tgcgccacca cctgcgcatg gcccgcatca aggtcggcca gatcgtcggc
     1021 cgggacacga agcacctcga cagtccggaa gtgacgagag cgacggtgga aacgattgcc
     1081 gaaaataccg tcgacggcat cgtatcgccc ttatttttct tcgccctcgg cgggctgcct
     1141 ttggccgtcg tctatcgggc ggccaatacc ctcgattcca tgatcggcta taagaacgac
     1201 cgctacctct acttcggccg cgtcgctgcc cgcatcgacg acgtcttaaa ctacatcccg
     1261 gcaagactga ccggcctctt attcatcgcc gtcgctgcca tcctgggcca tgactacaaa
     1321 caggcctgga acatcatggg ccgcgatgcg aaaaagcacc ccagccccaa tggcggttac
     1381 gctgaagcga ccgtggccgg tgccctcggt atccgcctgg gcggccacaa tcgctatttc
     1441 ggaaaagata ccttccggga atacatgggc gatgccggcc gcgaattgga accgatccat
     1501 attaaacaga ccatccgcct gatgtacggc gcgtccctgt tggcaggggc catgacggcc
     1561 ctcttgtcct gggcacggta cggcctatgg agctgatcgt ccgcgtaccc ggttcctgcg
     1621 gggaaatcat gcagggctat tggcagggcg agcccttttt ggtgacctgt cccatcgacc
     1681 ggtacggtta cggtccgttc ggggacggga cgccttgtcg gcggcggggc caaggcgaaa
     1741 cgcgcttttt ctctggccct gacttactgc cgggtatccg agctgcccta tgatttttac
     1801 cttacgtctg atctcccgga aggcaagggc atggcctcga gttcagccga tatctgcgcc
     1861 gtcctggccg ccgtcggctt cgttttagga cgccccttac gtgaacaaga gattggccgc
     1921 ctggccgctt ctatcgagcc gaccgacggc attttttgtc ggggcatggc cgtcatcgag
     1981 cccgatacgg gacgcatcaa ggccgtattt tcacaggttc ccaagctttc catcgccgtc
     2041 tttgactccg gcggcatcgt cgataccgtc gccttccacg aaaaggggcg gcgccgaaga
     2101 catcccgagt caaacgccat ggcggccggc ctggccctct tacaggaccc acttacggcc
     2161 gagaccctgg ggcgggcggc gaggtacagc gccctggcca atcaggctct cctcgaaaag
     2221 ccggactttc cggccttcgt cgaaagggcc ctccaaaagc cctgtgtcgt cggtgtcaat
     2281 acggcccata gcgggaccgt tgccggtgtc ttttttaccg aaggggaggg ccttgacgtc
     2341 cgagcggaca tcgtccgccc cttgacggct gcctttcccg actggaccta cgtcgatacg
     2401 gtccgtctcc aagagggcgg catttttatg gaaaggaggt gataggcagt gcttaaacga
     2461 tttgaacatg gcggcaatat ttatgcggct ccgccgccgg ggaaggcctg gctcgatttc
     2521 agtgccaaca tcaatccact gggactctcg ccggatgtga gagatgccat gacggctcac
     2581 atagacgccg tcgtccacta tcccgatccc aaggggacgg cccttcggga agcccttgaa
     2641 agaacgtacg gcgtctccaa agattctctc ctcctgggaa atggggcagc cgaactcttt
     2701 tatctctata tgcatacgtt tcggccccgg cgggtcttga ttcccgtgcc gtccttcagc
     2761 gagtacgaac gggcggcacg ggcctgcgga gccgtcattg actactgtca tttgtcggcg
     2821 gaaggggatt tttcttatcc ctgggaaacc ctgcgctctg cctgtggtca cgtcgactgc
     2881 atcattatcg gcaatcccaa taatcccaca gggactttgg cctcggctca tgagctgaaa
     2941 gcccttacgg atcttgcggc ccgcaagggg acggacattc tcgtcgatga gtcttttctc
     3001 gattttctcc catcggaaga ggcttattcc gtccgagaac tggcacagga gacggattcg
     3061 ctgttcgtca ttcgatccct gactaaattt tatgccctgc cggggctgcg cctcggtttt
     3121 ggcgtgacga cgcccttacg gcgaagtctc atggaaggcc ataaagacga ttggaacgtc
     3181 aacgtcctgg cccagtatgc cggcgtcgcc gccttagggg actaccaaaa cgcgtcgcgt
     3241 cgcctcgtcg ccgcggaacg cgatcggctc tatgaggcct taacccatat gcccggcatc
     3301 gaagtctggc ggccctgcgt caacttcctc ctatggcgcc tggtcgacgg atccttggca
     3361 gaaccgctca tatccgccct tcgtgaagag ggaatcttag tccgctcctg tgccaattac
     3421 gtcggtttat cgccgtccta tataaggaca gccgtccgtt ctcctttgga taacaaatcg
     3481 tttatacag
//
LOCUS       DAWDXZ010000136         3478 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6158_length_3478_cov_8.109553, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000136 DAWDXZ010000000
VERSION     DAWDXZ010000136.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6158_length_3478_cov_8.109553"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1329..1338
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2023..2032
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          887 a          826 c          909 g          836 t
ORIGIN      
        1 gaatgcggct ctggcggaac ttgccgccgg aactcgtggt taaggtaaag ttctgcggcc
       61 tcaagcggcc ttgcgacatc gtttgggcca atgaactcca ccccgactat gccggcttcg
      121 tcttcgccgg tacgaagcga cgcgtttctg atgagctggc agcagaactt cgcagggaac
      181 tggatccgtc catcccggct gtcggcgtct ttgtcgacga tgagccgaag catatggcgt
      241 cgttagtaaa aaaaggcgtc atccagcttg tccagctcca cggtcaggaa gatgaagctt
      301 tcgtacagca gatacagacg gatttaggcg ttcctgtcat caaggctttt tctattgcca
      361 atcaagacga cgtaacgcga gccttggaga gccgtgccga ctatatcctc ctcgatcagg
      421 gaaagggcgg tacgggtaag gcctttgatt ggtccttgct gaaaggcatt ggccgaccgt
      481 actttttagc cggcggcctt acgccgcaga atgcagttca agcagcagcc cttcagcctt
      541 atgccctcga cgtcagcagc ggcatcgaaa cggatggggt gaaggacaga gaaaagatga
      601 ctttatttat gacaacatta ggtgtttaca ggaggtaaac agatgagtac aggaagattc
      661 ggctcctttg gcggccagta tataccggaa acgctgatga atgaaattca gcgattggaa
      721 aaagcctacg accattataa acatgacccg gcctttcagg cagaattaaa agaactcctt
      781 gataactatg ccaatcggcc gtcactcctt tactatgccg aacggatgac gaaagatttg
      841 ggtggtgcga aagtctattt gaagcgtgaa gatttgaacc acacaggggc ccataaaatc
      901 aacaatgcct taggtcagtg cctgttggcg aaaaagatgg gcaagacccg ggtcatcgct
      961 gaaaccggtg ccggtcagca cggtgttgcg acggcgacgg ccgccgcttt cttaggcctt
     1021 gaatgtgaag tgttcatggg cgaagaagat acgattcgcc aggctttgaa cgtctatcgt
     1081 atggaactgt tgggggctaa ggtccatgcc gttacttccg gctcccgagt ccttaaagac
     1141 gccgtcaacg aagccatgcg agaatgggtc agccgtgtcg atgacactca ttacgtcatc
     1201 ggctcgacca tggggcctca tcctttcccg atgatggttc gcgatttcca ggccgttatc
     1261 agtaaggaag cgcgtcagca gatcctcgac aaggaaggga aattaccggc agcggtcttg
     1321 gcctgcgtnn nnnnnnnnaa gatgtccgtc tcatcggctg cgaagctgcc ggcaaaggca
     1381 tcgataccaa actccatgcg gcgacgattg ccaaggggga agtgggaatc tttcacggca
     1441 tgaaatcgat tttttgccag aacgaagacg gccagatcga cgaagtctat tccatttcgg
     1501 ccggtctcga ctatcccggt atcggaccgg aacacgccta cttaaatgaa acgggtcggg
     1561 ctcagtacat tccggtcaaa gatgatgaag ccgtcgaagc ctttgaatat ttggcccgca
     1621 cagaaggcat catctgcgcc attgaaagcg cccacgccgt ctatgaagcc atgcagctgg
     1681 caccgacgat gagtcctgaa gacagcatca tcgtctgctt gtccggccgc ggcgataaag
     1741 acgtcgctgc cattgcaaga tataagggga gggatttaca tgagtaagat tggccaggct
     1801 tttaaaaagg gaaaagcctt tatcggattt ttgacggcag gtgacccgtc gttggaaaag
     1861 acctatgaat acattatgac catggaaaaa gccggagctg acttgattga aatcggaatt
     1921 cccttttccg atcccattgc tgaaggcatc gtcatccagg aagcggactt acgggccttg
     1981 aaacaaggga cgcgcatcga cgacgtcttt gatctcgtta agnnnnnnnn nnttaagcgc
     2041 gtccggaagg attcgcagat ccccttggta ttcttgacct atctcaatcc cgtcttccat
     2101 tatggggccg atgccttctt cactcgttgc gaagaagcag gcctcgatgg catcattatt
     2161 ccggatatgc cctttgaaga acggggcgaa tgtgcagccg ctgccaaggc tcatggtatc
     2221 gatatcattt ccatgattgc tccgacctca aaagaccgga tccagaccat tgctaaggcc
     2281 ggggaaggat ttttatacat cgtatcctcc ttaggcgtaa ccggaacaag gacggaaatc
     2341 aagaccaacc tcaaagacat catcgatacg gctaaagctg tgactgacgt tccctgtgcc
     2401 gtcggcttcg gcatcaatac gaccgaacag gctgaagcca ttggccgtga cgccgacggg
     2461 gtcatcgttg gcagtgccat cgtcaaactg attgctaaat acggggacga tgcagaaaaa
     2521 ccaatctatg actatgtgaa ggaaatgaag gaagcggtca tgcgcagccg ctcataatta
     2581 ggccatatag gaatttacat aaagatctga atgacagttc gtcgctgtgg ttcagatctt
     2641 tttttatgaa gaatgcttat ttattacaaa ttggcataaa aaatagtctg tctatgataa
     2701 atttggcact tcgttgactt tgtcgatttc ccatgataat atgtaggcaa gatatttctt
     2761 gtaacgaaat atttatataa ctaaggttta tgcacgctat atggatattt ccatttcaac
     2821 agaaggagtg acgatcatgg attttgaact cacgaaacaa cagcagcaaa ttgtcgacca
     2881 agtacgcgat tttacggcta aacggttatt gcccggtgtt gtagaacgcg atgaagcttc
     2941 ggaatttccg ttggaagctt ttaaagaatt tggcaggatg ggcatgttcg gtctctgcta
     3001 tccgacagaa ttcggcggca gcgatgtcgg ctatcttccg tatatcctgg ctgttgaaga
     3061 agtttccaaa gtagattctt cctttggcat cggattttcg gtcaatacgt ctctttacgg
     3121 cggcagtgtc atgtattctt cggcgacgga tgaacagaaa aaacgctttt tatcccccat
     3181 cgcatcgggc caggccatcg gctcctttgg actgacggaa gcgacggccg gttctgacgc
     3241 tgccggtcag cagacgattg ctactaaaga cggcgacgac tatatcttaa acggtactaa
     3301 aatttttaat accaacggtc ccattgccga ctataccgtc gtctacgctt tgactgatcc
     3361 ggctatcggc ataaagggca tgtgcgcttt cgtcttggaa aaagggatgg aaggcttcca
     3421 tgtcggcaaa ctggaaaata aaatgggcat ccgctcggct caggtttcgg aaatggtc
//
LOCUS       DAWDXZ010000137         3477 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6161_length_3477_cov_5.609293, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000137 DAWDXZ010000000
VERSION     DAWDXZ010000137.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6161_length_3477_cov_5.609293"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1089..1155
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2487..2496
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          817 a          791 c         1049 g          743 t
ORIGIN      
        1 ggaactggct gcgcactggt actatgaagc ggccatggcc ggcgacgggc cggcgcagaa
       61 taacttgggc gtcctttacc gtgacggcca cggcgtcgcc cgtgacagca ttcaggcggc
      121 ttactggttt gcccagtctg cagcgtcagg ctataaagag ggcatgaaaa attacgccac
      181 cgccctggaa acaggcgatg gcgtagagtt gaatcgtgat gaagctcgat attggcgaaa
      241 taaaagccgg gacgattagt ggagaatatt gataaatacc gtaatgatcg tggcattgac
      301 cgggtcgatg attaaggacc cgatgagggg aatgacgaag aaggccgtcg gtgccgggcc
      361 gaagatttcc gtcatggccg tcatattggc gatggcgttg ggcgtagccc ctaagccgaa
      421 gccgcagacg ccgctggtca tgacggcagc ttcgtaatcg cggcccatga tgcggaagac
      481 gatgaagtag gcgaaaagag ccatgaacag ggcctggctg aggagcatga cgaccagagg
      541 tacggcaagg tccgccaact gccagagctt gaggctcatg aggacgcagg tcaaaaagac
      601 ggtcaggctc agattgccca ggacttcgct ttcatgctgg tagactttga agctgtgcgt
      661 gaagtcggcg atgttgcgga tgatggcagc gacgatcatc gagccgatat agcccgggaa
      721 gacaaggccg atgctgctga agaaaccgct gacgatcgtt ccgagaccca tggccaggaa
      781 gaggtgggcc atggcgttca tgaagcggta gttgtcgatg gtctggccga ttttgttttc
      841 aatcttgatg gcgttttcgg cccgttcttt atcgccggct acggctttta accgcgatac
      901 ggcgtcggcg ttggccgaag gatcgaaccg ttctgattca gccgagctga ccagatggtg
      961 tttgtgaatc agtccttccc caatcggacc gccgataaca ccgccggcga cgaggccgaa
     1021 ggtagcggcg gcgacggaaa tggtgacgcc gctgtcaagg ccgatgtctt ccaacaaggg
     1081 accgaaggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnncgatc ccgttgccag tcccatgaga ggactgaggt cgaagactgt
     1201 cgcgaggctc accccgatga tattctgtaa ggtgatgaga atcgtcgtga cgatggccat
     1261 tttaaagacc atgatgccgc ctcgtttgat aaggctgatg ctggccgtgt agccgacact
     1321 ggtgaaaaag agcatcatac agatgttttg cataatcgta tcctgctcgt actgccaaag
     1381 accctgtgta tagagaatgc agttgatgat agcgaataag atgcctccga cgaccggagc
     1441 ggggatacag tacttgcgca ggacgtcgaa ttttgcacgc aagaatacgc cggtatagta
     1501 aacgacaatc gctgcggcta atgtttgata aatattaaga tttacagcca caaataagcc
     1561 cccttcatat tttaccgatt atggtcagaa tcgatgaaat aggttatgaa aaaataaaaa
     1621 atacagtact tatgataaca gattttgggc tgtaaaacaa atatatggat aaaaataagc
     1681 ggaggttacg gttcacgatc gccttgggga agatagctgg gggaggtata atagtccctt
     1741 cgtccttggc ggcaagaagc gctgcggaaa cagagcgctt gaatgtcgtt ggcccggata
     1801 tcaagggtaa gctgtgcctg tccgagaggg gacaggattt tttttgcctg cgccggtttg
     1861 gttatgatgg ggaaggttcc ttttgctttt ttcaagaagg cggcgccgcg ctcgttgaag
     1921 gccaggatgc ggccgtattg aggcccttca cggtaggatt cattgaagaa atcttgtgac
     1981 ggctgcagga ccgtataggc gcccatgcgg cacaggcggc tgaatgcata gcgcttggtc
     2041 ttgacggcgg ccagggcttc ttcatacgtc gaaagggtag agaaggcccg gtgccaacgg
     2101 ttttcgaggc cttcggtaaa ggccggcagg cagcggagct gttccggtgt caagaggcgc
     2161 ccttcaaggc tgacgaaatc gccgtagcga tcgtagtccg tgaaagaacc ggctctcaat
     2221 agggcttcga tgtcgctgtg gttggccggc ggcaaatagg ctgcgacgcg ggacgtgaat
     2281 ccctttttca gttcccggcg gagagccgag gcactggtga aaggctggtc gaagctgttt
     2341 tcagcatggc ttccgcggcg agggacggtt tgcatcgcaa ggggagacgg cagggcgagg
     2401 atggcttttg catattcaag agccaagagg gcattgggct gatccaaaag gccggccagt
     2461 tccggcaggg tctttgccag gtcatcnnnn nnnnnntgat tccgaccggc cgtggccgtc
     2521 tttgtcgatc catcgtacga gaggctcaag gcggtttttc ccaggcggag aaagtcggca
     2581 gccgtcccct cttcgacgcc gcaggcaagg gaggtgcagc caaggcgata ggctaaacgg
     2641 atgccgcctg aggcaaatcc ttcggcactc gagagggcat agacggcagg cagttcaaag
     2701 acggcatcgg ctccgctgcg gacggcccaa cgggagcggt gccacttgtc gaagagtgcc
     2761 ggttcgccgc gctggacgaa ggcgccgctc agaatgacga tgacggccgt atcgggcgga
     2821 ctttggcggc ggatttccga cagcaaatag gcgtggccgg tgtgaaaggg attaaattcg
     2881 gcgatgacgg caatggcttc catagcagcc tccttacgtt ctaaatttca caatatctga
     2941 acgggacgaa gccgcagtgt ctggcggcac cgtccggaaa aggccgtatt tcacgaagaa
     3001 acacagtata agttgtttat aagtatacgg aaggtaaaga aaatcgtcaa tggatgccgt
     3061 tcattctttt tggctgtttg ggcggatagc taaagtattt ccaattcctg ctttttttag
     3121 atgacgctat aaggctttta tgctataatg aacctagtct gtagaaagca taaataggtg
     3181 attgtatgaa gggattgaaa ggacttgcag ctgcggccat ggtctcagct gccgtattgt
     3241 tagccggctg tggcagcaat acgatgcttg aagaatacag tttcggctat gtccagttaa
     3301 aggccggaaa ttcggaagtg tacgtcatga ccccgtttga cctggcacac gtgaatcgcc
     3361 aggacggcga aggcatgatg tatattaact atgacaaaca catcgatgtc gtcgctgttt
     3421 ctgcaacagc cggcgatacg acgcctgttg ccatggcaga taaatacgtt gccatgc
//
LOCUS       DAWDXZ010000138         3469 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6178_length_3469_cov_6.754247, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000138 DAWDXZ010000000
VERSION     DAWDXZ010000138.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6178_length_3469_cov_6.754247"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    480..489
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          990 a          775 c          846 g          848 t
ORIGIN      
        1 gtctgcacac ggttaaattg gtccatggta atcgactcgc cgttttgccg gtagaatgta
       61 aggctatcca ttgccttatt ggttagttgc ccgcgctgga acgtcatcat agcgacagcg
      121 gatccaaccc ggaattgata tgcttgtgtc ttagtgtcgc cgacttggtt ctcgtgtttg
      181 agctcgccat cgacgcccag ggcattccgg acagtgttgt agtcgctgcc catcggcagc
      241 gcgacaaatt tcttgtaagc gtccatttct acgctgtcct taggcttttc cggggcagcg
      301 tcttgttttt ccggctgact ggcttcctgc tgctgagcag gagcagaaga gggagccggc
      361 ggcgcggcag ccataccgat caaaccacat atgaataaca cgccgaggca gattagacct
      421 cgtttcttgc cgtatggtgc ccgaagaaat ggagcatact tttgtggatt tttcttggcn
      481 nnnnnnnnna aatgataaca gcaatctcga caagtcctat tacgatcata atatccataa
      541 taatatacct cccttaccaa gctctacgac tctcaattac cttgcctaat attctaaccg
      601 gcaagtcgat aatttgctgg ttagaataga aatgtggctc atatacgtct ggattaaatc
      661 catataacat aatgccgcct tccagctttt tgacctgctt gatcgtggca tcattgccat
      721 taactaatac aattgcaatg tctccagact ctacatcttc ttgctgctta acgatgacga
      781 tatcaccttc ctcaagcttt ggctgcattg agtcgcctcg tacctggagc gcaaaaaagt
      841 cacccgtagc tgccagtgcc ggcgttattt cctcatagtc taatatttct tcgacggctt
      901 cgatcggcac gccggctacc actcgaccga ggaccgggat gcggatgccg cgtccggcag
      961 gtcgctgatc tgccgatgac aataacgact ccagcgggac ccctgtagcg ttagcaatta
     1021 gccgcagtgt agttatactt gggttatatc ggtctttttc gatatctccg atatatgacc
     1081 ttgatagatc aaccgcttta gcaagctcaa cttgagtcat ttttgcaagt ttcctggctt
     1141 ccttaatttt ctccccaata cttttcattt ttttcgcctc ccttaaaaaa atacattgta
     1201 aggtatctcc gtctattaaa gatattataa tataattttg tcggaaatac cattgacaaa
     1261 atgacggaaa tactatacac tattggtgtg gacggaaata ccagtaaaaa ggaggtggca
     1321 atacgtgaat caaattggag atgtactaaa aaaggcccgc gaaaaagcgg gacttactca
     1381 gcaagaactg gccaccctag taggtgttag tcgggcctac tacgctgatg tagagcgggg
     1441 acgatacacg cccagcttaa aggtcttatc cagattagcc gaagtgctag ggattgactt
     1501 aaattttttg aaacggaatg acggaaatac aagtaaggag gcgtaaaaat gttagtaaag
     1561 tccttagtaa taactgccca ctggaaacta gcgaagtctg gaaaatatga agtcgctaat
     1621 cgcttgtatg aactgttaag aagaggttac gtgtgtttag acttaggtga tattgatgac
     1681 gaggcggctc gagtatgcga tgcaatcgga ctccattaca atgttagttt aagatctggg
     1741 atggctcgtt actggttaca gaggagagtg agaacatgaa agaagtggaa gtcgtcaggc
     1801 tggaacagct ctatgccagt ccgaaacact tggccgaact gtacgacttg ggcatgacct
     1861 gcacccgggc gcttatcgat gaaatgaggg ccagcaaaaa gtggcggccc agtgtcattg
     1921 cctatgacag aatggtccgc gtcaacattg cggattttga ggcgttttgg cggactaaaa
     1981 gcgttgtgta gaggaggcca gaaatgaaaa gactaatagg catgctaacc gtactagcca
     2041 taaccggagg actgctaggc taccatgatc ggccggcggc ggaggcatcc ccgacggtgt
     2101 acgtcgtcga gtcaggcgac acgctatggg atatagccag gcctatagct gatgcccgcg
     2161 gcgtcgacat ccgggacgtc atctgtcagg ttatcgctga caatgatctt ggcatcgacg
     2221 ccgtcatcca gccgggtcaa aaaatcgtta ttaagtaata ggaggacaaa gaatgggaca
     2281 atggaaagaa cttgttagat gggccaaaca agagcggcta tataaccaat tatcatctgc
     2341 cactaaaagg gccgaggacc tagactggga tggggatagt ggagttattg tggccacaaa
     2401 tatcaaggag atagataatg cccttatata cgccaagggg aatcccgcta ctattatcca
     2461 attaatagcc atcctattaa ggcgttttgt gggggcgtat ccggaggaaa aacaaaaaga
     2521 agttatagaa gacattatga aaataattaa tcaattatag gaggaagaac catgaccaaa
     2581 aacgaacaag cacggctaac tactgaattt agggctcact gccgcgaagc tctaaaggcc
     2641 caggccacgc aacgagaaag tgggtacaat ttaaaaccct tacatgtagc tattataaaa
     2701 gctgctgaag cccgcggcta cgggtacgca ttagtcgaca tgcttgcctg gaaaatggat
     2761 atgtcagaaa tacagagcta tatcaacaat ttaatcgcag aatcagagga atatatggga
     2821 attaaaaccg gcttgcggtt gacgccatgg accgaagagg aggcggagaa atgacgcacg
     2881 aagaatatca ggaaatcgcc caggccatta aaaaagtcca taaaatatca gtcaacgacc
     2941 atgagggcat cgttatgatc ttagcctctg aaaaggagga ccgtatcatc caattattta
     3001 acggagactc agccgtcttg gtcccaacat taagggactt acttatccat atcgtcaatg
     3061 ggttaccccc agaagacaag cttatcgcta agcagtctat tattgaggcc gctgaaacta
     3121 ttgagggagg tgacggcaat ggacacccag aaactccgca aacgactgcg gaaaacccaa
     3181 ggcgacatcg agaaagctaa gtttggcaaa aacgatgctc ttatcttgct ggcgtcgctt
     3241 gacgatgggc aggacgagtc agaagtccat gctatcaggg gacggaatat tgaagttttt
     3301 gcgcttatcc tgactgcgat acaggtcata ctagatggct atgacgacga tcatgacgcc
     3361 cgtgacggca tcaaaaaaat tatgttagcg gctatcgagg cattataaaa ggccgccccc
     3421 tgcgccaaca gggaagcggc ccaaaccaaa ctacttatat tgattatac
//
LOCUS       DAWDXZ010000139         3453 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6196_length_3453_cov_7.327251, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000139 DAWDXZ010000000
VERSION     DAWDXZ010000139.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6196_length_3453_cov_7.327251"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1456..1465
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          805 a          870 c          907 g          861 t
ORIGIN      
        1 taatttttga cgtcgacggc gtcttactca gcgaaaaacg gtattttgac gtatcagctc
       61 tggtcctttg ggaatggtat tacagtgaaa aatacttgca tttgggtcag gagacgatta
      121 cagccgacct cagtgatgaa gccattgacg gtctgcgccg gcgtttttgg aaggacgatg
      181 ccatcttgct gtggctcaaa aaacacggcg tcaacagcaa ctgggatatg gtccacgccc
      241 atctcgtcgt cacactgtgg ctccttttag aatcgtatac cgcacatcac gacgccttga
      301 atatggcctt tgagacaccg gctgatattc aatccctagg cagacgtctc tggggctgtg
      361 cggttccttc ggctgaagcc gtttatcagc ggctggttga aacggtgccc gatgaggccg
      421 gaaaggacga cgtcttcact tacctgagcg atgccgttgc ggcttcctta ggagagggga
      481 cgcgccggtg gacgcctctt cattcgccgc tttggaatct ggcctttgaa accttccagg
      541 attggtactt tggcgatgaa ctctatgaaa aaacctatgg gaaaaaggcc tatgcaccgg
      601 gaaaatcggg atttttgact cgagaagaac cgctgggcac ggtccaaggc atccgccata
      661 tgttccaaga actcaagcgt cgaggctacg ccattgccat cgccaccgga cggtcgcggg
      721 cggaaatgga aattcccttc cgcacctacg gctggctgaa agaattcgac gagcattacc
      781 tggcgaccta cagtgacgtc gaagaagctg aaggcctgtt gaacttatcg cttgataaac
      841 cgaatccttt cgtctattgt ctgggggcct ttggcaaaga taaggcccgt tacggcgact
      901 atgtacgccg catggatgat ttcaaacagg gaacgtatta tgtcgtcggc gattccctgg
      961 ccgacgtttg gtgtgccaag gccttagggg cggtcatgat cgggaccttg accggcatcg
     1021 atggagatga cgccagggcg atgtttgatc gagaaggtgt tccgcatatc gtgccgaccg
     1081 tcgaagctat tttagatttt ttagtatagg acttatttat gaaaattact gcaatcgaaa
     1141 tcgggaaaat tgccattcct ttaaaaacac cgtttattac ggctttgcgc cgcgtcgatg
     1201 tcgctgaaga cttagtcgta aaagtgtaca ccgatgacgg ccatgtcggg tacggcaatg
     1261 cgccggcaac ggtcgtcatc accggcgata gtcatcagtc ggtggccgga gccataaaga
     1321 atgtcatcgg tcctaaactg actggcatgg atatcgatga ccgtgacctt attttagaaa
     1381 cgattcaaag ggcgatggtc cacaatacat cagccaaggc agcccttgac attgctgtcc
     1441 acgatctctt tggacnnnnn nnnnnttgga cagcgctacg gcatgcccct ctatcgcttc
     1501 tttggcggcc gccgccaacg tattgcctca gaccttacga tcagcgtcaa tccgccggac
     1561 gtcatggctg tcgatgcggt aaaagccgtt caggccgggt accatcattt aaagctgaaa
     1621 gtagggattg acagtactgt cgactttgag cgcgtcgcag ccatccgcaa ggctgtcggt
     1681 aacgacattg ccatccgcct cgatgccaac cagggctgga aacctaagga agccatccgc
     1741 ctcatccgcg ccatggaaga tgccaatctt gctattgaac ttatcgaaca gcctgtaagg
     1801 gcagccgatt ttgaaggcct caaacaagtg acggatcatg tcgatacgcc catcatggcc
     1861 gatgaatcgg ctttcagtcc gaccgatgtt tttcgcctgc tttccatgcg ggcctgcgac
     1921 ctcatcaata tcaagctcat gaaggccggc ggcctcggcc cggcttcaaa aatcgcggcc
     1981 atggcagaag cggccggcgt cgagtgcatg atgggctgca tgttggaatg caaaatcggt
     2041 atcaccgcag cggcagcctt atcggcaggg acagccgtta ttaccaagaa tgatttggac
     2101 gccgccgacc tcatggcagc agaccccatt cacggcggta ttacctatga taaagaccat
     2161 ctcgtcctct cggaacttcc gggcctcggg attcaagaca ttgaaggctg gacccctttt
     2221 taacgaaaag ggtccggctt tttctgttga cacgatggct gacatttttt ataataaaaa
     2281 aagtatcata cagtttgtta aggaaccctt ccggcttgtg cagcaggtct gaagggttct
     2341 tgtacgatga atccatttat gaaatcgagg aggatacatg cgtttacgaa gaaaaccgtg
     2401 gattgacgag gccattaagg agtacagtga gtttctccac ctcgacgatt gtcaaatgta
     2461 taaggggaag tggcatacgc tctttgcctc cccggaagcg ccgctgtggg tagaacttgg
     2521 gaccggcaag gggaacttca tttcccaatt agctcagatt caccgcgatg tcaactttat
     2581 cggcattgaa atccaggccg gagtccttta ttatgccggc aagaagtgtg ccgatgctga
     2641 agtcgataat gtccatctcc tgcgcttcga tgtcgcccga ttggaagata tttttgaacc
     2701 tggtgaagtc gaccgcttct tcatcaattt ctgcgatccc tggcctaaga agcgccatgc
     2761 taaacggcgc ttgacctacc gcagctttct cgaccgctat gcccgcctct taaaagaagg
     2821 cggtaagatt tactttaagt ccgacaatgc cggcctcttc gattttaccc tcgacgaatt
     2881 taaagcctgc ggatggcctt tgtcggaagt gacctatgat ttgcataaca gctcagtact
     2941 gaatgaagcc atgaccgaat acgaggctaa attcagtgcc aagggacagc ctatttttca
     3001 ttgtgttgcc acacgcccac aggaagtgat agctcatgtt gtcgaaaaag atgaatgccg
     3061 aaatgaaggc ccggaggagt aacctccttt ggatgctggc gtccatatcc ctcttgatag
     3121 tcattggcct ggaaaatatc ctcagctcga cttttgtcct cgatgaagga gcctccttgt
     3181 ccggttattt gatcaagcag ctgggctttt taggcggcgg cattgcagct gcctgggtca
     3241 tttggcgatt ggggtatcaa ttcattcgcc gctggtgcag caagattgtc ctgatcaata
     3301 tcatctgtct cattgccgtt aaattcgtcg gtattacggt aaacggtgcc cgtcgctggc
     3361 tgggattcgg tgccttttcg tttcagccgt cggagtttgc caagttagcg gcgatcatca
     3421 cgatggccgc ggccattgcc ttttatttgg aac
//
LOCUS       DAWDXZ010000140         3451 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6201_length_3451_cov_7.067138, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000140 DAWDXZ010000000
VERSION     DAWDXZ010000140.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6201_length_3451_cov_7.067138"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    320..329
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1469..1478
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          808 a          950 c          936 g          737 t
ORIGIN      
        1 tcgactacga aggccatcac atcaacatcc tcgatacgcc gggccatcag gacttcagtg
       61 aagacacgta ccggacgctc atggccgccg acagcgccgt catgctcatc gacgtcgcca
      121 aaggcgtcga agcgcagaca aaaaagttat tccgcgtctg taaagaacgg ggcatcccca
      181 tctttacctt tgtcaataaa atcgaccact tcggccgcaa ccccttcgac ctcatggatg
      241 aaatcgaaga catcctgggc atccacgcct accccatgaa ctggcctatc ggcctcaacg
      301 gcagctacca gggcatttan nnnnnnnnnc tttgaaaagg acacgaccca cggccagaga
      361 aaattagcct cgaccaaggg ctcggccgac gatccgattt tcaaggaact cctcgaggaa
      421 gacgtccacc agggcctcat cgacgacatc gaactcctcg acgaagccgg tgatgaattc
      481 gacatggaaa aggtcaagcg cggcgaattg acgcccatgt tcttcggctc ggccatgacc
      541 aacttcggcg tcaagccctt cctcgaagaa ttcctgcgca tggccccgaa accgcagccg
      601 cgaaaatgcc tcgaaggctc cgtcgacccg acgtcggatc agttcacgtc cttcgtcttt
      661 aagattcagg ccaacatgaa tccccagcac cacgaccgca tcgtcttcat gcgcatctgc
      721 tcgggcaaat tcgaaaaggg catgtccgtc acccaccgtc agtccggcaa gacactgcgg
      781 ctcatgcagc cccagcagtt cctggcaacg gaacggacga tcatcgaaga tgcctacccg
      841 ggcgacatca tcggcgtctt tgacaacggc accatgggcg tcggcgacac cttatacgac
      901 ggcaaaaaga aggtcacctt taaagacttc ccgaccttcc cgccggaact cttcgcccgc
      961 atccgcgcca aggactccat gaagcgcaaa cagttcctca agggcatgac ccagttagcc
     1021 caggaagggg ccgtccagat ctatgaaaac atgggctcca tggaaagcta catcgtcggc
     1081 gccgtcggcc aattgcagtt cgaagtcctc gaataccggc tgaagcacga atacggcgtc
     1141 gatttagaaa tgaaccgcct gccctacacc gtcgcccgct ggatccagga caacggaaac
     1201 gactataagg acctcaagaa tatcgacagc gccatgatcg tcaaagacca taaacagcgc
     1261 gtcgtcctgc tcatcaccaa cgaatggcag gccaactgga tcgtcgaacg gaatcccgat
     1321 ttcaccttct gcacgacgcc ggaccaactg gccatcgaag acgaagaaga ataatcacaa
     1381 tcatcgtaag cacgctgaaa agaccggaaa gaaatggaat gcacttcttt ccggtcttct
     1441 ttatatgctg aacaagataa gggttgatnn nnnnnnnnca gactaagcga cggcgcggca
     1501 ccattccggc agggtgatgg tgcggtcgaa ggccggcagc caaatgccct cggtatgatt
     1561 ataagtccga acgaggatgc ggtcgttgta aagatagata gagttggaat agatggtatc
     1621 ttcgtcccag tcgctgttat agacgttgag gacccggcct tcataatagt tctggtcatc
     1681 cataaaggtc gcatcgtccg gcatggtatg ggtgtgtccc gatacccaca ggcggacctg
     1741 gtggtggcgg gccaggatgc ggcggatgtc ggcggcgggc tgggcggagt tgcgcggcga
     1801 cggcaggctg acgccgttct tttcgggaac ggctgtattt tctaagggag cgtgacagaa
     1861 gatgatcgtc ggcatctgac ggtgagcggc caaagtttgg tcgagccagg tcagctgctg
     1921 gtgagataat tcgacggcag cgccgccggt aaccgtgtcc ggcgacagga agacgaggag
     1981 atagccccgc tgttccttgg cataataaag gggcccgaag gtcttggtat agcgggcaaa
     2041 gtgcaggatt cgttccgtac tgctgcaggg ccggagatcc tcgccgtggc ccgtatagag
     2101 cagttcatga ttgccggcaa tgaaggcttt ccgctttttc aggccgttga cgacgagccg
     2161 caccaggcgg tattcttcgg cactgccgtt acgctggacc atatcgcccg taaagacggc
     2221 taaatcaagg tcgcgccagt cgttcatatc ctgcagggcc ccctctttgg cagccatata
     2281 gcgcaggcgc tctttcggcg tgaccagctt tttacacgga taatggaaat cactaatgat
     2341 ggacaggcgc gaataatttt ttctttccag gtcgtctcga ttcctggaaa acagtgacga
     2401 aaacatggca tctcctcctc atccccaata aaaacaggct ctcgctgtac atactaagta
     2461 tagcgagagc ctgtataact tttgcaaaga ctggtggaaa tgtctgtaaa aggacgcgtt
     2521 ctataaggtc ctttcagtct caattatgcc tattcttctt ccggataagt caggccccac
     2581 tgctgacgga tggcagtcat gaggtccatg acgtctttcg tgtattcctg gcacatgccc
     2641 ttgccgccgt tagcaacggc ctgttccatg tcgaggactt cgtactgcag ggccttggcc
     2701 gtgctgcctt cccggatgac ttccttgcgg ccgtcttcgg tgtaagtaat agtcgcttct
     2761 tgagcccggg gatagtcgta gatttcgata tagcccttat cgaaggcggc catgcctcgt
     2821 ttcggctgct tggcgtgaag ggacagggct aaggttgcca tttccccttc tttattctga
     2881 aggaggatgc ccgcctgttc atcgacgccg gtcggggccc gcaggacctg agaggcgatt
     2941 tggtcgggct gagccgtcat gaagtagcgc acgaaggaca gggcgtagac tccgatatcg
     3001 aggagggcgc cgccggctaa gctgcggttg aagaaacggt tcttcatatc gtagtcttta
     3061 tagctgccga aattcatctg gacgaagcgc agtttcccta aagcaccgga atttacgagt
     3121 tcagaaagtt tcttataaat cggcatgtgg tacaaggtca tggcttcggc caggacgacg
     3181 cctttttcgt cggcgacctt acgggcccgg gccagttcgt cactgttgag ggtaatcgac
     3241 ttttcacaga ggacgtgctt gcccgcttca agggccggaa tcaggtactg gatatgggta
     3301 ttatgcggcg tcgataagta gacgatatcg acgtcggggt cggaaaagat gtcttccggc
     3361 ttgtcataaa ctttggaaat gccgtatttt tcggcaaagg agacggcttt agcatgggtc
     3421 cgattgccga cgccatacaa gtttccccct a
//
LOCUS       DAWDXZ010000141         3438 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6218_length_3438_cov_4.788649, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000141 DAWDXZ010000000
VERSION     DAWDXZ010000141.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6218_length_3438_cov_4.788649"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    695..704
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2356..2365
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          851 a          736 c          864 g          967 t
ORIGIN      
        1 aatcagttat tgacgaaaaa cttgcagtaa gtcacggtct atcaaaaggt catcaccgga
       61 agtttccggc ggtggccttt tttggttcta taataaatgt tggggtctgg tggacactcc
      121 ggttcacaag accccttgtt ttatataaaa taagacatat ccaacactac cagttagctc
      181 aaccaccgcg aagaactgaa aggagtatat agatgtgtat cttacctgtt ttatcgaaaa
      241 atacttcaat tgggaagagg aaacggtcaa aaaattagaa aatcacgagg gagaatgagg
      301 cctctgcgcc ataaaaatac tggcggaatg atttttgata ggacagggga aaggccggaa
      361 ggcagggaac tttttcggag caggctttat gaatggccaa cgaaaaaacc agccctcggt
      421 caggatggct ggttgatctg tgttttcata agaaagggat gttctccttg gccttgaaaa
      481 ggcgtagcgg ggagcgttgt tatccccatt acccttcggg gtgaatgagg gcctgaacgt
      541 ccgcagcagg cggcaattca ccttcttcgt caccggcgta gaggatggaa gcgccgaagg
      601 tgtcgccctt cagcagggct tctttggcga tggggcccgt gggatggggg atgacgacgg
      661 cgtcgtggtg cttttcttta aaggtcttgg cggcnnnnnn nnnncgtcga tatcggcttc
      721 tgatgcgaca gggacgacga gttcaaaggt cacggcttcg agatcccagc gcttttccat
      781 catggcgacg agatcggctg ctttagcccc tttttcgccg cggatgacga tgattggtac
      841 acagccgaga tccacggcct gaccggcaat gtcttccagg ttgtcgtcgt cgaggctgcc
      901 ttcaatcttg acggcgtcga tgagggacga gcggatggca aagcgcagga tcgatccgta
      961 atcgcggtcg gtctggtaca tttccgggag cttgctcttg tcacaggaaa agaggaccga
     1021 cggattggcg gcttcgcggg cgatgtacag caaggcgttc caaagctggg ataaattgac
     1081 ggcaccgtta aaataaccgg catcaaaggc catcatatca taagggtttt ctacaatctt
     1141 ggccattcgg atctgaaggt cctgaatcgt ttccccttcg aggggaacgg cgataaaggg
     1201 atggtccgat tcaaagtgaa tactcattgc gcttggcatg aaacattacc tcctacgtaa
     1261 aagactacct ttattgtact cgttatgagg acggttggca atgcctgtcc tttgtggcag
     1321 gagaagaaaa acgttgacaa gttatgctat atcttgtata atattttttg ttggcggggc
     1381 atagcgcagt ttggtagcgc acctggcttg ggaccagggg gtcgcaggtt caaatcctgc
     1441 tgctccgacc atacgaagat gcgggtgtag ctcagtggta gagcgccagc cttccaagct
     1501 ggttatgtgg gttcgattcc catcacccgc tccatttttt attgactcag tagctcagct
     1561 ggatagagca acagccttct aagctgtggg tctagggttc gaatccctac tgggtcacca
     1621 acttgaatcg atacgaactt ttcgcggttg cagcagactg tgattgccgg ggagttcgtt
     1681 tttatcatta tatcgggcag tttttcgtat tcggctgccg gcaggcgaca tccctgccaa
     1741 gaaaggtcct tgccataaga aaaccttgca tcgaccttat gaatatggta ttataaataa
     1801 gtgctcttta gagcacattt attcatgcgc ctatagctca ggggatagag caccggtctc
     1861 cggaaccggg tgcgaaggtt cgaatccttc taggcgcacc aaagaaacaa gcggtttcac
     1921 agaaatgtgg aaccgcttgt tttgtttggt gtgagttttt ggtgtgaatt tcagggaaaa
     1981 atatatacag ggtgaaatcc atggaagcga cgacttaact ttttagccaa ctatttttgc
     2041 cttcaggata tgctgaatac atcaggagtt gtcaggctgc cgataatttt tttatgtttt
     2101 caggctttag gcgttctgaa aaaagaatat aatcggtccc cttggcgagt aaggcatcca
     2161 ggtatttttt atcgacgcct ttttcatcgt tgatgatggc caatcccgtt acctttttac
     2221 gggcgctctt ttgccgctca tcccgtatgt ccagccaatc gaacaggaag gcggctaagt
     2281 tgcctcgatt gattgaattg aaggaattga taagtatttc tgaggttctc ccggcaatct
     2341 gaaagttgaa attgannnnn nnnnntcctt tggctagaaa gttgggcgtg taaataagct
     2401 gctgttggtc aaagtaatcg gccacgtcat ccaagaagat tgtttttacg gtggtgttgt
     2461 tcagcataaa catatcgttg acggtaagca tggcttggat gaacatgtgc atactggcag
     2521 catagttttt cgtgtctgtg tgcataatca tctcattgtt ttgattttcg acgccataag
     2581 aaaaaagaag ttgatctaaa atgacggctc gtttagaacc gcgcagagag actccgtcag
     2641 caagcaagtc gttaatgatg tacccgtcat cggataaggt gatagcgtta ccgtcccgtt
     2701 ttgcatagat taccaggccg tcattatggc ggtccatgaa cggcgtccct atttctgtcc
     2761 agccattttt caaattccgg gcagataagt tcttctttat ccattggtaa tattcggaaa
     2821 tccagtcgaa attagtatcc atcaaaaagc acctccggtt ctgcaagatg tacttgatct
     2881 ttattgatta cgtttgaata ggccaagaag tccataaaca agccgatgat atcgtcggta
     2941 tgattaaact cattcggcag aggatacgcg aatttgtctc cgtgcccttc tttgtatata
     3001 tggatatggg gctctaatgc ttcgattctc gtcccatcag gattcgtatg tgaggtaggg
     3061 tcttgtgtat caatccttac caatattata ccctgcgcat agagcagacg aaaagatact
     3121 ttcttgggat tttttttgcc ccgaaaagcg gagatggtca tatcatcttt tcgacggccg
     3181 tctctttcat accataacgg gtataattta gtttcccgtc gttccggtag ccggatgctg
     3241 gtcgggttta tgaaagcctt ttctatttcg cgcaatgtat ctgcttctcg ttgcgttaat
     3301 atcatcatga acatccttcc acattaaaat atcttaattt tcccgatgta ctaaaaagcc
     3361 ccactgcata ggcagtggga ctagtgcccg acacataaaa tgtgctgaag cttttccttg
     3421 tctatagtct atcataat
//
LOCUS       DAWDXZ010000142         3419 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6248_length_3419_cov_7.569560, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000142 DAWDXZ010000000
VERSION     DAWDXZ010000142.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6248_length_3419_cov_7.569560"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1447..1456
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2451..2460
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          774 a          859 c          968 g          798 t
ORIGIN      
        1 gggctcggag caaatcgccc tcgcatggaa gcccccctta gtcgggaagg gaaacgaggt
       61 tcgtaaacag gtctttttcc tggcgaatcg tcgcttcact gcccatgacg cacaggttat
      121 catccttcat gaccgcttcg atgagcggag aaagggcctt aatatcggct gccgtcgtcg
      181 ccaagagctg atcgcggcga gcctgggcga cttcgtccgt cgtccccgta aagtaacggg
      241 tcatggcttt ttcaccgcga agggacggcg tcaaaggcac atcgatgcgg ctcagcgtac
      301 cgatgacgta tttcgtcatt tcccggtccg atacgtcaaa ggtcttaaag tagtccgcca
      361 agccgtcata ggcttcgacg ctggaacgca gattcgggtc ccggtaagag caaaatacca
      421 tgtcgccgtt gtcatagaaa cgagtgaaag caccgtaggc ccctccctga acgcgaacct
      481 tggtccataa atagccgtac tggagaatcg tatcgaggac catcagcgcc ccggtatagt
      541 cgtagccgtg gccgcggaag ttgccgcctt tggcgacgta ctgaacggtg ccgctggtca
      601 tgatgccttc gttcttcgcc gtaaggccga agtcgcaaag accggtccgc ttttctccct
      661 gcggcagggc ttctgtccag cgagccgcac tggccttagc accattccaa tcgctgtcac
      721 tggccgtcat ttctaaggtc agggccgagc ggctgaagat ttttttcatg agggctgcca
      781 atttcccgcc gatttcatcg gcatcggtct tgatgcgttt ggcaatgtcc gtgataaatt
      841 catagtacga gaattctccg gcatcgcaga acgcttcgac cggcgatacg taggacaaga
      901 cgcggtgcat gacgatcgta tggccgcggc ggaaggtatc catatcccag ccggccttga
      961 tttcatcgac cagttcgatc aatcgttcct ggttggtaaa gaccgtttcc aaggagattt
     1021 cccgcaagag gtcgattaat ttgtcgaagt taagggccaa gcccttggct ttaatccgga
     1081 atacgggcgt ataatcgctg ttgtccaaac gattggaata ggccgacaca ctgtagccga
     1141 tgccgccggt gtagagatcg ataagggacg ccagttcgcc gtacgtatgg gacttggtgt
     1201 cgatgtcacc gaggatgtcg ctcaaaagat aggcgtaaga aatttcttcc cgcgtcaggc
     1261 cgtgaaggtc gaagtaggca ttgacatagg taatgccgtt cgtgttgtcc ggtacatggc
     1321 acaaggtcac gtcgccgagc tgttcctcat gcatgaccac cgtatcgact ttcttttcaa
     1381 gatcatcacg ggataaggtt ggaatcgatt ccagggcttc cggcgaatcg ggcgtcgcct
     1441 gacggannnn nnnnnntaag gctttatcgt gtttttccgt caggcccggt tccggtacca
     1501 gcgataccaa tgcgtagtag gcattatcga ggatgtaacg ctggatcaag tcttcaaagt
     1561 aaccagtgtc aatgccctta cggaggacat cgagggattt ttcataagac aaggcgtcta
     1621 agggatcgag gtcatagagc caggtatcca tgcagcggat ggcatagata aggcccttag
     1681 gacggccggc aaaatcggct tcacggaggg tgaattccgt ccggttcaaa gccccggtca
     1741 aaagcgtctt gtcgagaccg tctttgacca tgcggcacaa aacgtcggtg acgataggca
     1801 tgattttatt ctgcttatcc gggtcagagc cgttgacaga gatgctccac aagggctgga
     1861 ggatgccatc ctggaagtcc ccggaaatgt ccttcccaag accggcgtcg acgagagcct
     1921 tcttaagagg cgcagccggc gactggagaa ggacataagt caggacttta aaagccaggg
     1981 ccaccgtcgg gtcgagggcc tggtcgatga cgtaggtcag gctgtgcagg gtcttgtgtt
     2041 ccgtcttttc cccggaggca atgccatagg gataggactt tacggcgctg cccgggaaga
     2101 cctgacggcc aatggtcgaa tcgacgtcct ggaggcggaa tttgctcaag tattccccat
     2161 caatgaaggc cagtgtctgg tcgatgtcca tgtcgccgta gaggaagata tagctgttgg
     2221 ccggatggta gtacgtgccg tggaatttga cgaagtcttc ctgggtcagg ttggggatat
     2281 cgtcaggatc gccgcccgat tcaacgccat aggtcgtatc ggggaagagg ttttccatga
     2341 cgtgccgttc gagctgagag tctggagacg aaaagacgcc tttcatttca ttatagacga
     2401 cgccgcggta aatgaggtcg ctgtcggccg aatcgaggtg gtaatgccag nnnnnnnnnn
     2461 ccttcctgca tcaagacctg tttgtccttc agcatattcg ggaaaaagac ggcatccagg
     2521 tagacatcca tcagattttt gaagtcggcc gcattgcggc tggcgacggg atacatggtc
     2581 ttatcgggga aggtcatggc gttgaggaag gtgttgagcg agcctttgac cagttcgacg
     2641 aagggttcct tcaaagggaa tttccgggag ccgcaaagaa cagagtgctc gcagatatgg
     2701 ggcacaccgg tgctgtccga cggcgtcgta cggaaggaga tggaaaagac cttattgtcg
     2761 tcgtcatttt cgatatagag caggcgagca ccggactggt cgtggtgcag ttcataagcc
     2821 tggctgtgga gttccggaac gtactgcgaa cgctcaacgg taaagccgtg aatcttctgt
     2881 ttttcttgta acttcataaa aacgctcctt atcatcataa tgttttaaat aatataacgt
     2941 atatataatc agtatatatt tataaggcac tgccactaag gccatcaccg tggatcattc
     3001 agtcagcgag cagtatccgg cccaattctt ctacggtatc ggcaatatag tcggggccgt
     3061 actcttttcg ttcggcagca ttaccgtagc cgtatccggc atagatgacg ggaaggccca
     3121 gttcatgggc gccgatgatg tcgaatttac ggtcgcctac catgacggct tccccttttt
     3181 caacggggcc gatgcccttt aaaatccggg ccatgatgtc ggccttggaa tcagtgccgc
     3241 caacggcagg accggcaatg gcttctaagt acggagccag tccgaacttt tcacaaatcg
     3301 cttcggcgtg gacctggggc ttcgacgtcg ccacaaaaga acggatgccc ttctccttta
     3361 atgtctttaa taaagggacg atgccttcaa aggcacggtt ttcaaacttg ccgatcgtg
//
LOCUS       DAWDXZ010000143         3397 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6283_length_3397_cov_8.713046, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000143 DAWDXZ010000000
VERSION     DAWDXZ010000143.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6283_length_3397_cov_8.713046"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    927..936
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2409..2522
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          788 a          880 c          885 g          720 t
ORIGIN      
        1 ggactcaaaa gtgcggcaaa actatttggt atccgcctcg ttcccgtcgg gcagcaggag
       61 ggagaaatga gtgaagacgg cctttcctat gccttaaaga atcatcatat caagggtttt
      121 tacgtgacgc cggactttca gaatccgacg acccgtaccc tgtctgaagc gggacgaaaa
      181 aggctggctg cctttgccca aaggcacggc ctcttcatca tcgaagacgc cattgccgcc
      241 ttgtttacgg aaaagccgat gccttcgatc aagaccctgg cgccggaaca gacggtctat
      301 atttttagcc tgtccaaggc gattctgccg gctttgcggt tggcctatgt cgtgccgccc
      361 agaaggcttg aagagcagct ttcttcggcc ttatacacgt tgaatctttc tcagtcggcc
      421 ctcctcatgg aactggctgc ccgcctcgtc gtatcgggaa agtgcggcgc cttgatggct
      481 cttcgacgcc aaagcctgcg ggaacgcaat gagctcgctg atcggatctt acgcggctgg
      541 cccttagccg gtggggccga ggccctctgc cgctggctga ccctgcctga gggactgacc
      601 ggtcaagtct ttgaagcact ttgccgtgac caaggcgtac gccttttagg ggcggaacac
      661 tttgctgtcg gtaccgatgt tcccgtcaac ggcgccagac ttgctattgg ggcgccgtat
      721 gataaggcgt ctttagaaaa agggctccat atcataaaga aggtcttaga ttcctatcga
      781 acataatcgt ggggcataca attatgttat ggtgtgcctt tttttcctat gctatcatga
      841 aggcaggaaa agagattttt ggaggaggga ttcttatgaa atgttacgtc gtcgatgctt
      901 ttaccaaaga aatttttaaa ggcaatnnnn nnnnnnctgc gttttggatg cttggctgcc
      961 cgatgaattg atgcaggcca ttgccatgga aaacaacctg tcagaaacgg ctttcaccgt
     1021 aaaagaaggg gacgcctatc gccttcgctg gttcacgccg ggtggtgaaa tagacctctg
     1081 cggccatgcg accttgggaa cgagctttgt cctggctaac ttcgtcgagc caaaggtcaa
     1141 tcgcttcgtc ttccataccc tgagcgggga tttggtcgtt acccgtaacg gggacctgtt
     1201 ttccatggat tttccggcct acgatatggt accgacgccg gtcaccgatg ccatgaccga
     1261 cgctctcggc ataaggcctt tagaagctta tatggcgcgg gatttggtct gtatcctgcc
     1321 ggacgaagat gccgtgagaa attatcagcc ggacttagag aaaatcaaag gcctcgacgg
     1381 catgctttgt cacattacgg caccgggccg agaatacgac tgcgtcagcc gcagctttgc
     1441 tcccaagctc tccgtgcccg aagatccggt ctgcggctcc ggccactgcc atattattcc
     1501 tattatggcg aaaaagcttg gaaaacaaga tctcctggcg tatcaggcat cgagacgggg
     1561 cggagaactc caatgccgac tggaaggcga cacggtgtcc ttggccggtt atgccaccct
     1621 ctattccatc gccgatttga aggtgtaacc cgtcggcgcg tcttgacagg cttaggtaaa
     1681 cttgctacaa tatttatcac aataaaggcg atgagcggaa gaagctttac ttctttttgt
     1741 cagcgaaatg ccgattcggt gagagggcat tcaaagagaa taaggcgcgt cacccgtgag
     1801 ctggcaggag gaaaggcctg tccgtcaaac agacgttacc tgtgccatga agcggtcgca
     1861 aaagcggcaa tttaggtggt accgcggaag atacagcatt ctttcgtcct aaggaatgaa
     1921 cccttaggcg agggaatgct cttttttgtt ttggagggaa taagcatgaa atggaataaa
     1981 gaacaactgg gcacgtatgc accgaatgag atggaaatga cgtggtacga tttttgggaa
     2041 aagaagggct acttccatca agaaccggat aagagcaaga aaccctttag catcgtcatg
     2101 ccgccgccga acgtaaccgg ccagctccac atgggccatg ccctggacaa taccctgcag
     2161 gatatcctgg tccgcttcaa gcgcatggaa ggctataacg tcgcctggat tcccggcacg
     2221 gaccatgccg gcattgccac ccaggtcaag gtcgaacagc agttggcgga agaaggcaaa
     2281 aaccgctatg aaatcggccg cgaagaattc ctgaaacggg tctgggcttg gaaggaacag
     2341 tacggcagcc gcatcgaaaa acagattcgc cgcctgggtt cgtcctgcga ctggagccgc
     2401 aagcgcttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnatacctgc gcccgtgccg ttcgggaagt cttcatttcc ctgtacgaaa aaggcctcat
     2581 ctatcagggc cagcgcatta ccaactggtg ccctcactgc catacggcct tgtcggatat
     2641 cgaagtcgac cactctgacg tagccggcca tctgtggttc gtaaaatacc ccgtcgtcgg
     2701 tgaagacgac tacatcatga ttgccacgac tcggccggaa acaatcatgg gcgatacggc
     2761 cgttgccgtt aaccccaatg atgaccgtat gaagaagtat atcggcaaga aaatcatcgt
     2821 gcccctcgtc gaccgcgaag tcgaagtcat tgccgacgac tacgtcgata tcggttttgg
     2881 taccggcgcc gtcaagatta cgccggctca cgaccccaac gactttgaaa tgggtcagcg
     2941 ccataacctg gaatcgatca tgatcatgaa cctcgacggt acgatgaatg aaaaagccgg
     3001 tgccaaatac aatggcatga cccgcgaaga ctgccgcaaa gccgtcgtcg ccgacctcga
     3061 agaactggga ctcctcgatc atattgaaga attgacccat gccgtcggcc attgctcgcg
     3121 ctgcaagacg acggtcgaac ccttcgtcac taagcagtgg ttcgtcaaga tgaagcccct
     3181 taccgaagcc gccctcaaag ccgtagaaga cggaaagacc cgcttcattc ccgaacgctt
     3241 cgtcaagacc tacaaccatt ggctggaaga cgtccatgac tggtgcatca gccgccagct
     3301 ctggtggggc catcagatcc cggtctggta ctgcgacgac tgcggcaaag cctcggccag
     3361 ccgtgaagac attacggaat gcccgcactg ccacagc
//
LOCUS       DAWDXZ010000144         3377 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6318_length_3377_cov_7.578868, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000144 DAWDXZ010000000
VERSION     DAWDXZ010000144.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6318_length_3377_cov_7.578868"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3073..3082
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          857 a          869 c          691 g          950 t
ORIGIN      
        1 gttcagccgg ttttatggta ccgcagccaa aaagagtctt caacatagcc agctggcgaa
       61 tggcatcttc ccggcacaat gtattattgc caagaatttc gttttcaatg ccttgcatgg
      121 atttattatt gtcgcacata gctcccatgt acttgttgcc aacaactaac gcgccctgct
      181 tagcgcaata agaagcaccg acaactttca caccccgctg tccgatcttt tcaatatggg
      241 atgtaaagtc aacatggtta ttgccaaagc cttctgtcgt aacaatggca ccatcgacgc
      301 ccaagcattc tacggtctga ccaacacgtc cggatacata gaacttatcc gagttagctt
      361 gtggtgatcc gaccagcata acgccgacta agtcgatttc ttcatcgtgg agggcttcta
      421 aaaccaaggg ttcacgccaa tagtgacgag acatttcctt agaggccggt ccaatgcaag
      481 tcaaagcatg gatgcatccg tcaaggactt ctaagggatt taccataacc ggaacattcc
      541 cgaggtcgac atttggctta gcccccagcg taccaaccgg ttctaccggc agcagcaggt
      601 tatcatgcat agccccttgt cccataattt ccttaacgat gacgacttta ggcttgcccg
      661 gtcgacgata ttgggtcagc gtttcttttt cagttaccag tgattcatcc gcttttttga
      721 gagcttcccg aatttcctgg caaatataat cagaagcccg atgagctgct aaggggccgg
      781 ggcgttccat attcgcatgg gcacgaatcg tgacattcgt ccgaatgaag atttcgccct
      841 tatccgggga accggggcgt ccccacatga tattgcggtc catttcgcct tcactggagc
      901 caaactcgcc gatctgtaca ccatcatgat ccgtaccggt aaccatgaaa acgactccgt
      961 cgaggacgcg ggttatacct tctccgactt cgccttctac cttggtagca atcggttgta
     1021 cgtccataat agtattgctg tactcaccat agcggtccgg cgtaataata tctaattcga
     1081 tgttttcaac tagttcttga gactcaactg cttcttcgca aattccgtct ttgcggattg
     1141 tcaaagttgt gccttcaatc tttgtttcat caccaaaaac aactttttct attttaacat
     1201 ggtcatgacg aacttcacga acctttacag cttcttccac ggcttcggga atatcagcac
     1261 ttgaatcggc aggtgctgcc gcagcgatgg attcagctga tttgaccgat gtcgtattcg
     1321 ttaccgtcgg agtcacaact cctgcggata cgccaccgat aggaaattct aagtcaattc
     1381 cctttccttc actgatgtga attttaacgg ttcctgctgt agtaacagct tgagaaacgg
     1441 gcaaaggttc tgtcattgga acagacgctt cttcttcaat tacatccttg accggttcgc
     1501 tttctgcagg tgcttcgaca gcagccttaa tgccatcaac catctctgcc gtcaaaggaa
     1561 ctaaagaatc ggcggtctgt tttaaagtag ccccaataac ctgttcaatt ttcagaacat
     1621 tatcgggaat ggtcaataag ccggaatctt ctaaatccgc aaaaattgcc ggatcctcta
     1681 aattagcggc ttctaataca gtgcctgctt cggcacggca gcataatact gcagggtctt
     1741 ttgcgtggtc ttttgccatt tcggctgtaa tagacataaa gtcatcctcc ttttaaactg
     1801 tttaatgttt aaacatcatt gaggaagctt tcgatacagc ttgtgaccgt cagcccgcag
     1861 tacatgatca gtatgatgat aaactggcac tcctaaccta ggagctacac ccataacggt
     1921 gttcgttcaa ttagagcatg ttccaacaat taaaacgtct gctccagctt ttttcaaagc
     1981 gattaccgtt tctgcctgcc ccgggcattc gcctggcaag tcacatctgt atcgaccatt
     2041 tacctcaacc attcgcttgc attgtttctt agcgactacc gttgcgccca tactttcggc
     2101 aatgtgcgtc aggcgttctt cggatccgcc gcactcacaa atcaagatgc cggccttacg
     2161 agattcttgt gggaacggca tggcgattaa gaaagctcca ctggtttttg ttaccggaga
     2221 atcttcactg ccggcacggc cggtctgagg accaccaacg acgatttcac catagggttt
     2281 tatgtacccg ccggccttca tgatcagttc atgagccgtc gttccaatcg ggacgtcgac
     2341 aaatacgctt tccttctgct ccactcttcc cgatacggtc aaatctttat caatcgccgg
     2401 ttttctgtct tcgatagctt ccgcgatatg cttaatcgtt tccacattat caatgcaagc
     2461 tccgacagct cccggcagtt gtcccggttc caggacaata cccatgattt cccgaacaat
     2521 aacgcgttca tccccggcag gatacatatc cggcaggcgg aagacatcga tattcggctc
     2581 atccttcgtt tccttgatta aggcaacgac agccttctta tgcttgggtt tgatagcaat
     2641 ataggccttc ggtgctttgg ttatttccat gcagtactta atgccgcgaa ctaactgtgc
     2701 agcctgtgtt tccatctgat ggatattatg ggccagcagc ggttcacatt ctgctgcatt
     2761 ggcgataaat acaccgttcg gaatctcagt ttttaactta acggctgtcg gaaaaccggc
     2821 accgcctgca ccaacaaccc cggcagactc aatggcttct aaattcgtct gaaattcagg
     2881 aataggcaca tatgtcgtga aatcctgaac atcatctgca gccatatgta tatccgtatc
     2941 cgtcaccttc gtgatagtac cggaataact ggcatggatg tttgctccca atccgccggg
     3001 aacggcaatc agctgaccgc gttttacata ttccccttca gaaacaatgg ccttacaagg
     3061 agctcccaca tgnnnnnnnn nntcaagttt gtgcttttac gaacaagcat aactaaagac
     3121 acttcatctt aaataaatta tctcatgcgg tatcatttga aaaaatagga cgatattgac
     3181 caattgttga cccattattg actcaacatc gatttcttat accgtaaaaa tggacatttg
     3241 ttagacaacc ttatataata agaactatcc tatatgataa tgtttcacac attaatcaaa
     3301 tactgttatt agtaagttag gtctatatgg atttttaaaa atactccgag gtaggcacca
     3361 tgaaaaaatc aaatgcg
//
LOCUS       DAWDXZ010000145         3369 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6324_length_3369_cov_6.961678, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000145 DAWDXZ010000000
VERSION     DAWDXZ010000145.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6324_length_3369_cov_6.961678"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1666..1675
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          706 a          893 c          964 g          796 t
ORIGIN      
        1 agcggtcaaa accggccagg aaacctttta cggtcttggc gatggtttca catccgccga
       61 cggtcttgct ttcgatgtta aggggcatat tggggtcgtg gagggacatg cgcaggaggg
      121 cccagccgcc gtcgaatacg aggcggacgc cttcataaga cggtttggca atggcaatgc
      181 ccttttcttc ggcccgctgt tcaaaggcct tgaggacgtc agccccgtag gcctgtacgt
      241 cgccgtcacc ggtgatctta aggcgatatt ccacgccttc cgcaggctgg ccgagggctt
      301 tgatgaggtc gcctaagggt tttccggcct tcttggcttt ggcggcagcc atgaggagtt
      361 tgacggccaa ataagcgccg tcgtcgaggt agtaattttc gcttaaggcg ccgtggcccg
      421 acgtttcgat ggccaagggc gatacggtgc cggccttgtt gaggcggata cattcgtcga
      481 tgacgttctt atagccccgc atgtaacgat gatggacgag gtgcagttcg tcctgaagga
      541 aggtcgtcaa ttcatcggac gtgaccgagt cggtaatgat ggtactgccc ggatagtccg
      601 gcgccaaaat ggcagccatc atggcgatga gggcgttgcg gttgatttct tccccactgg
      661 gcaggacggc gctcatgcgg tcgacgtcgg tatcgaagat gaggcccagg tcggccttgt
      721 tgtcgaggac ggctttccgg atggaggcca tggcttcttt gttttccgga ttggggatat
      781 ggttcgggaa gtggccgtcc ggttcgagga actggctccc cgtcgtatcg gcgccgagag
      841 gtgccaagac cttcgtcgca aagaaaccgc cgctgccgtt gccggcgtcg acgacgatgt
      901 gcatcccttc taaggggcgg tcgaacatgc cggcacagag gccggcgcag atcttctgac
      961 gcaggtggag ggcgtagcgt tcaatgaggt ccaaagaggc gatggctgcc gtcgatacgg
     1021 ccgttgccgt aaggttcgac gccagcgtca ggacttcttt gacctccttc ttttcaaggc
     1081 cgccgtcctt ggtaaagaat ttgaggccgt ttcgattgta cggcaagtgg ctggccgtga
     1141 tcatgatgga cccgaacatg gccggcgtcg atgccagacc gcagtccgta acgtaaacgc
     1201 cccggccgag gagaccggca aagacggctt ctttcatcat atcagccgag atgcgcgagt
     1261 cgtgaccgac ggcgatgcgt aagtcccctt ccttttttcc cagcttctgc gctaaaaatt
     1321 tggcaaagcc ggcggcaatg cggttgaccg cttccggcgt caggttgacc ttttcgtcag
     1381 ggacgccttc gacggcgatg ccgcggacgt cactgccatt ctgtaatttt aagatagctt
     1441 cggtagagtt catatgcctg ttcctgctgc ctattcccga taagacagct tttcctttct
     1501 gtaatctaca tctatcatac tgaaaatcgg cccctgacgc aaggcgtgca gggacctgtc
     1561 ccttataata cgtatttttc gacggcttca gcgacggcgc cttcgttgtt ggtagcttcc
     1621 gtcacgacgt cgcagtacgg gcggatggcc ggattcaagt tgcggnnnnn nnnnntcgtt
     1681 tcttccggac gcacgccgag gatgtcggcc aatttcaaga ggccggcgcc cttattgacg
     1741 cctttatgca tgaattcgat gtagcggttg ctggagaagc tgaccgtaat gtcgtcgagg
     1801 aggggtttca attcggcatg aactcgttcc aaatagtcgt agtccgtatt ggtcaggatc
     1861 aatttcccga cttcttcttt accgcggata aaatcgaggg tcgtttcttc gcagggcgtg
     1921 taggccatgc ggcccttcag gaaggcttct tcgtcaggcg taatattggt gatgtagacg
     1981 acgtcccgcg tatagatgtg caggcactgg ccgtaggaag cacacttgca aaaaatctga
     2041 tcggccaggt caaagggaat agggtcccag aaaagggagc ggctgccctt attttccgtg
     2101 atgttgccgc cattgaggcc gatgacgtac tggtcgggct gttcaaaaag acggagtgtc
     2161 ttcaagacgc cgtcaatggt gcgaaaactc cgacccgtgc agggcacgaa tttgacgccc
     2221 tttgcgacag ccttggcaat ggcttcgata ttgcgctgac aaatcgtcgc atcgctgctg
     2281 agcagcgttt catccatatc acaagcaatg attttatagt tattcatgac ctactccttt
     2341 acaaatcgca tgacgggata gttactaact gatactacga acatatcatg tctgaggcat
     2401 tcccgccacc ccagggacag aatcagtccc attatagtca aaaaagaagg gcttctgccc
     2461 ttcctttttt atgatcgatg ccgccggtcg cggatggccc gccacacagc cgacacggcc
     2521 ttaaacagca cataaatgag tactacatta aacaacaggc cgagaaaatc ggcggcccag
     2581 ccaaagttcc ccatgccaaa gaggctgctc aacatgctgc ccaggaacat gcccccggca
     2641 aagaggccga tgccgcgcat agcgccgccc caagccgacg agcgattggc tgtcgtattg
     2701 gcgttggccc gaggcgcagc cgtctgattg cgagtctggg attgtgccga tgaagcgtta
     2761 ttattcgtat tggccttagg cgccgctttg ggagccgtca ccgccggtgc cgaccgcggg
     2821 gccgacatgc gaataccgcc ttttgcagct tggaccggct ggtacacagg cgccgcaaaa
     2881 aaggccgtct ggagaagtaa gagacaagtc acgcaaaaaa gctttttctt catggtaatt
     2941 cctttcctat ctatcgatga accggaacgg cgtccggcgt tattctgctt tctctgccgg
     3001 aagcggatcc gttttttccc ccgaagcaga gatcaaagtg tgtaagggga tagccgccaa
     3061 gttttccgcc agtttatcct gaatggctgc ataggcatca tgggtcgggc aagagccgtg
     3121 acggcggcga tacaggcatt caaagttgcc gtcgaggcag ctgatgatca tgttatcccg
     3181 atccataacg gtgataacgt ccatcaggtt caattcatgc aaatctttca ccagtcggca
     3241 gccgccttgg gctccgcgga tgatttcaat atatccggcc tgttcgagct tcttggaaat
     3301 cttgtaagca aactgacgag gaatcagctg ttcatcgctg atttcccgaa tattcttctg
     3361 ttcccaatc
//
LOCUS       DAWDXZ010000146         3331 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6393_length_3331_cov_8.340049, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000146 DAWDXZ010000000
VERSION     DAWDXZ010000146.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6393_length_3331_cov_8.340049"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1241..1250
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2458..2467
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          832 a         1004 c          859 g          616 t
ORIGIN      
        1 caaggccaga tccgctgatg ctgcgcttcc ttcgcggtta cgggcggacc cttcgccaat
       61 atgccgcctc accgaagacc cgttttgaat accgacacta tgccaagttt ttattgcttt
      121 atgcgttagc cgtagctatc gcctggggag gaatctattt gtaccatgga aatcactagg
      181 ctcggcgtca tgggagggac ctttaatccc atccacttag gacacctcat gatcgccgaa
      241 gaagcgcgtc aggcctttca cctgaagaag gtcctgttcg tgccgtcgta cattacaccc
      301 aataaggatg tctgcggtgc gacagccgag cagcgattgg ccatgacgcg actggctacg
      361 gccgataacc cgtactttac cgtcagcgat atggagatgc gccgccgggg caattcctat
      421 acggtcgata cgctgcggct tttaaaagaa atatacggcc cgtcgcacag cctgtatttc
      481 atctcgggga ccgatacgat ccacgacctg cacaactgga acaatccgga agaaatcctc
      541 agcctgtgcc agtttatcgg tgcgacccgg ccggacggct cggaacagat cgattccatc
      601 atctcgtcct ttggcgagct gggaaaaaac atcctcaaac tgcccgtgcc gaccatggaa
      661 atctcttcga cagaactccg gcgccgcatc cgcctgggcc tgtccgtccg ctatatgatg
      721 ccttcggcag tcgtcgaata catcagaaaa aatggagtat atcaatgtac aacgaagaat
      781 tcaaagatgc cattaaagca gatttaaaaa aatccctgac gcccggtcgc tatacccatg
      841 tcctcggcgt ggccgcagcg gcccggcact tagccggcct ctatggcagt gatgccgatg
      901 cggcagaagt ggctgccctc cttcatgatg cggcgaaaca aaagcccctc gaagagatgc
      961 aggccttagc cggccgttcc tttacggatc ttccggcgcc cgtctttaac atcggcagcc
     1021 tcctccacgg ctatgccgcc gtgaccctgg cccgggaaca gtacggcatt gacgatcccg
     1081 atatcctctc ggccattgcc catcacacga cgggggccga aaccatgggg aagctcgaaa
     1141 agatcatctt catggccgac tatatcgaag tgaaccgcga ctttgacggc gtcgaagcgc
     1201 tgcgtcaggc cgccgaacgg gaccttaacg aagccgtcct nnnnnnnnnn gctacgattc
     1261 gacgatccgc cacctcctcg atcagaagaa accgattttc atcggtacgg tcgtcaaccg
     1321 caacgcccac atcagcgcca tgatgaaagc ggcagcgagg aaggccggcc gatgacacga
     1381 gaaaagagaa gaagaatctt aaaccgcatt ttagccgtcg tcgccgccgt ggcgattttc
     1441 ctggcctgcc gctcggccta tgttcatttc accagtcccg aaacgcagcc cgaagggccc
     1501 gatacgacgc cggccggcag caacgacttc gtccagacca gccccgataa gccgctctac
     1561 gtcctgctca tcggtacgga cggagaaaat ccccagcagg ccaacttcgt cggtgtcgcc
     1621 gccatcaaca aggacaagaa gcacatcgac ctcatcatgc tgccggacga tacgaaaatc
     1681 gaagggcgca aggaaaaggg gatacaagaa ctgcaggacg tctacaccga aggcggcatc
     1741 agcctggtgc gggccgtcgt ggaagacatt ttccacattc ccattcccta ctacgcagcc
     1801 tttacgccgg aatccttcgc caagatgatc gacatgaacg gcggcgtgcc cctgtacgtc
     1861 gaaaagaaca tgtaccacgg cgatgcagcc ggcgaaacgg acatcaacat cttccagggc
     1921 taccagaccc tcgacggcaa ggacgccacg gggtatatgc gctacctcga cagcaacggc
     1981 tacgtgtcgc ggacccagcg ccaggaacgg ttcatcaaga acttctacga agaccggcaa
     2041 aaggacttcg gcctgagcaa cgccttcttc atgtaccgcc tgtggcacaa cgtcgattcc
     2101 aatatcccgg ccaaggacat ggccgaactg gcctttgact tccgcagcgt cacggccgac
     2161 aacatcaact tctatatcct gcccggcgaa atggctccgg gcggtaagaa cggcgacaat
     2221 aaatattact ggacctacga cccggtcgaa gtccaaaaag tcatcggtcg gacgaacaac
     2281 gccatctcgg cagcgcccaa gcccgatgat gcctaacctt acattcgtat aaaaaaggag
     2341 acttttcata tatgccaatt accatgaaac ccgccaacgc ggaaccgatg aaagaattat
     2401 ccccttatga aatcgtctgc gccgcagccg acgacaagaa agcctgggac atcaccgnnn
     2461 nnnnnnntga aggcaagtgg atcctcatcg acgccggtga agtcattgcc cacgtcttcg
     2521 tcgaacagga acgccagtac tacgatctgg aaaacttgtg ggccgacgca ccgcgaatcg
     2581 actatgaagg tgaataatat gacgacactt caagaaaaca gtattgctac cctcgaagtc
     2641 ctgcgccaaa gcgaaatggg tgcctttttg gacggcggca cgggcaatac caacgacgac
     2701 atcctcctcc atatcaatca gcagacggaa gaggtccata tcggcgatgc cgtgacggta
     2761 ttcctgtacc acgacccgtc gggacgcctg acggcctcca tgcgcctgcc caagattaag
     2821 atcggtcaga tcggctatgc accggtcatc aatacgaccc gcttcggagc cttcgtcgac
     2881 gtcggtaccg aacgcggcat cttcatgccc tttgctgaaa tgaaaggccg ccccaaagaa
     2941 ggggaatacg tctgggtcaa gctctatgaa gacaagtccc accgcctggc cgtatcgatg
     3001 gaagtcgaag atgaaatgcg ccgggcgtcg agagcggcga cggacgctaa ggtcggcgac
     3061 tgggtcgaag gggccattta caacatgacc gaccaagggg cctacctcat gacccgcgaa
     3121 cggtggatcg cctttttgca ccgcagcgaa ttcaacggcc ccatcctcat cggacagatg
     3181 ataaagggcc ggattacctt cctgcgcgaa gacgggcgca tcaacatttc cctgcgccag
     3241 acgaaagaaa atgccatcaa ccccgatgcc gagaagatct tgcagttcct cgaacagcgc
     3301 aagggcaaga tgccctatgg cgacaagacg g
//
LOCUS       DAWDXZ010000147         3318 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6406_length_3318_cov_9.194606, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000147 DAWDXZ010000000
VERSION     DAWDXZ010000147.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6406_length_3318_cov_9.194606"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1656..1665
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          848 a          894 c          847 g          719 t
ORIGIN      
        1 tttacttctg atcctgcatg tcgtttacag taatttagtg ggcaaggtcg ttccgggatg
       61 gacgacgatg atgatcgtcc aatgcctgtt cagcggcatc cagctcattt ttatcggtgt
      121 catcggagag tatataggac gtatctttga agaggtaaaa cataggcccc tgtattggct
      181 gcgtgccgaa ctgggtcaac gaaaagaacc ataaatgcct ttgcgttgta tggacttcga
      241 gacagagagg agtttttatg tggagaaaac acagtaaggt gtgtatattg actgcccttt
      301 tcgtatgtac ggctttgccg gcactggctg cttcgacgct gaccgtcggc agtcggggcg
      361 atcaagtccg cgctgttcag cagggactgc gcagccaggg ctacaaggtc aatgtcagtg
      421 gtgtatatac gaaagaaacg gctaaggccg tgtcgaaata tcagaaagat aagaaaatcc
      481 aggtcagcgg cgacgttggc ggctggacct attatctcct gacaggcagt cggtccatgt
      541 tacctgccaa tccacccaag aataatcccc cagtcgctaa gaaagcacaa gctatcaagg
      601 tcaatgacaa ggccgtatcg aaatataaac actttggcgg cggtgatgcc tttgggaata
      661 aactggtcaa ttcagcgtac cagtacctgg gcgttcccta cgtatttgga ggaaacacgc
      721 ccgatggttt tgactgctcg ggatttacca agtatgtctt ttcccataac ggcattgaac
      781 ttccgcgtat ggccgatcag cagtacgaag tcggtcagcg tgttcgtcgc aacgacctca
      841 tgccgggcga tttagttttc tttacgacgt atgaaccggg tgtatcccac accggaatct
      901 atgtcggcga caacaacttc atcagcgcta ccagcagcgg tggtatccgc gtagacagtt
      961 tagacagcgg ctactggtcg tcgcgctacg tcggcgctac cagagtccga aaataagatg
     1021 taatggctag ggaacaagtc gtctctagcc attccttata cggcagtcct tgagattttt
     1081 atcgtcaaaa ggttgccgaa ccgggggaaa tgagcgtata attttcccat atagaaagat
     1141 atgtagaggg agtgtttatt aactatgaag tacatgacgg gtaatgaaat ccgcaaggcc
     1201 tatttacaat tttttaagag caaggaacat ctgatcctgc acagttttcc cctcattccg
     1261 gaaaatgacc ccagcctgct cctcatcggc gccggcatgg caccgctcaa accgtacttt
     1321 acgggcaagc tcgtcccgcc gtcgtaccgc gttacgacga gccagaagtg cattcgcacc
     1381 ggggacatcg acaacgtagg ccgcacggcc cgtcaccata cgttttttga aatgctcggc
     1441 aacttctctt tcggcaatta ttttaagaaa gaagccattt cctgggcttg ggaattctta
     1501 acggaagtac tcaaactgga caagagcaaa ttgtacgtta ccatttatcc tgatgataaa
     1561 gaagcttatg attactggca taatatgatc ggtcttgccg acgaacggat cttcaaattc
     1621 gacgacaact tctgggaaat cggcgaaggc ccttgnnnnn nnnnnaatct ggaacctcgt
     1681 cttcacccag ttcaatcgga cgaaagacgg gaagtacgaa cccctcgaaa agaaaaacat
     1741 cgataccggc tgcggtctcg aacgactggc ttcggtcatt cagcagaagg aatcgaactt
     1801 tgaaacagac ctcatcttcc cgattatcga aaaggtcatc gccatcagcc acggcgatta
     1861 caacgatcct cagcagaagg tcgctatgaa ggtcattgcc gaccacatcc gggccattac
     1921 ggtcatgatc tccgacggta tcctgccgtc caatgaaggc cggggctacg tcctgcgccg
     1981 catcctgcgc cgtgccgttc gtttcggccg tcttctcgga atccgcgaca tgttcctcgg
     2041 cagcctcgtc gatacggtca tggacatcct cggtgacgaa tatccggaac ttcgggaacg
     2101 tcaggacctg atcaaacaga tcatcgatac ggaagaaaaa caattcagcg ctaccctggc
     2161 tcaaggcatg gaactcttaa acgacatgat gagtcagagt gataataaag tcctctccgg
     2221 cgacgacgta ttcaaactct atgatacgta cggctttccg atggaactga ccgaagaaat
     2281 tgccggtgaa aacggtatga ccatcgacac cgaaggtttt gaagctgcca tgaaggaaca
     2341 gcaggaacgg gcccgtgcag cccgcgccga cgtatcggct aaagtcgcaa cgccggatac
     2401 gacgaaactc gaccagtcga aactggtcaa cgacccgtcg gcaacgaagg cgtccatcgt
     2461 catgatcggc aagggcggcg tcgaagtcga tacagcagca gccggggaag aagtgacgat
     2521 catcgtcgac aacaacccct tccacgccga aggcggcggt cagctcggcg acacgggcac
     2581 cctttccggt gaaaagggga aggctcaggt caccgatgcc aaggccctgc cgaacggttt
     2641 ggtatacctc atcgccacta tcgaagaagg ctcccttcag acaggcgatg ccgtcgacgt
     2701 caccgtccat cagacacgtc atctgaatct ggcccgcaac catacggcaa cccatctcct
     2761 gcaggcagcc ctgcgcaagg tcttgggcaa ccacgtcaac caggccggct ccttagttac
     2821 gccggaccac ctgcgctttg acttcaccca tttctcgccc gttacccaga ccgaactcga
     2881 tgccatcgaa aacttggtta acgaagaaat cctcaaaaac atacccgtca ccatcgaaga
     2941 aatgtccatc gacgacgcca aagaaaaggg cgccatggcc ttattcggtg aaaaatacgg
     3001 cgacgtcgtc cgcgtcgtct ccgtcgatga cttcagctgt gaactctgcg gcggttccca
     3061 cgtcgacaac acgtcggtca tcggctcctt ccgcattacc ggtgaatccg gtaccgggtc
     3121 cggcgttcgc cgcatcgaag ccgtcaccgg ggctgctgcc ttggctctgg ctaagaacga
     3181 atcccgtgaa ctgcagcagt tggccgatat gctcaaagcc aaagtcggcg atacgacggc
     3241 gaaattagaa gccctcctcg ctcaggccaa gggccttgaa aaagaattgg cccagcttaa
     3301 gaaagaagcg gccatgtc
//
LOCUS       DAWDXZ010000148         3314 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6414_length_3314_cov_6.273090, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000148 DAWDXZ010000000
VERSION     DAWDXZ010000148.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6414_length_3314_cov_6.273090"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          782 a          838 c          947 g          747 t
ORIGIN      
        1 atggccacga cgtcgtcggc tgtcgcatcg taagggatga aatacgtatc gggaaataag
       61 aagccttccg tggcaaaggt cacttgccgg ttgcccttca tatacggaaa aagctgggca
      121 tccgacgcag ccgcctttaa aaagtccgct tcactaatac ggccattggc agcgacggcc
      181 ttggcaatat tccataccgt ataaccttcg ggcacgacga gccggtcagc ttcagacttg
      241 ccgctggcca gtttctgtaa aatcgtaaag acgctcattc gcgagttaaa agtatattct
      301 ccggcgtgga tcgagccggc ctgaccggtc gccatggccg ctgcgcggaa ccataaagga
      361 cttgcaatat agcccttatc tgtcaaagca tcgccgacgt cggctgccgt catgtcactg
      421 cgaacatgga gatagccgct ggtatagggc aagaggaagt tcgggccgaa aaagccatag
      481 agcacgacgg cggccgcggc gatgaagcac ccggtcaata tttttttaag tctcttacgc
      541 ggaggcttag gggatggcac gggagctgtg tttttaattt ccttcgaggt catatcgttt
      601 gtttccatat tctcctttat caatcctttt tatcgggtta acggtcagca gtccaagtac
      661 ggctctgctg agccttttct tcactgcggc tcatggcctg ggcctgctgt tccttatcgt
      721 actgcgcctg ggcggcgtct ttggcagcct ggagctgagc ctgaacggcc ggatccggcg
      781 gaactacggt gccgtcttca tcgacggtct gggccgcatc gagctgagct tgggctgcgg
      841 cgacgttatt ggccaggttc tgaaggcgct ggttattgat gccccggcgc acatcggctt
      901 caagggatac ggacggcgtc tttttgacgt cgacgtagac ttcgaacagg cgctgccaag
      961 gcaaggtgac gtcaatggtc cgcggattga caccgaggta cgtcgatgca tagcgctgga
     1021 gctgtgtttt ggccaggctc tgcagttcct gattccgcga agcgtacacc ggcgtgatgg
     1081 tgtgggcttc gagtttttga tccatgcggg atacgtagtc gcggacgttg gcctgataag
     1141 cccagttatc gagatactgg gtggtcagca tgtcgcggtg gtcagcgggc gtcatatact
     1201 tggcgaggac gccctgcgaa aggccgtagc cgacggagtt gctggccgta ttccagccgt
     1261 tgtaggctgc caggcgatac atcatcttgt cctggtagag gccgtagacc aaggtgttat
     1321 ccgagccgtt ggaaaaggcc atatcgacga ggctaaccgg gatattgagg ttgaccgcct
     1381 tttcaatctg gttcaggaag tctttcgtcg actggagcat gatcgggaag ttttcaaaat
     1441 ttgccgattc cgtcgtcgtt gtagtcagcg gcgtattgac ggcgagcagg agatccggcc
     1501 gttcattgtc gacaatcgtg ccgccaatgg cttcgacgtg agaggcaata gtcttgccaa
     1561 tggcctgtcc gtcatagcgg gggaccgtct tttctccccc gcccaagggg tagatgaccg
     1621 aaattttcgg atgataatta ttgaagtcgt tgctggctct cgtaatgagc agcaatccca
     1681 actggtcggc gccggggaag gaaccgaagg atgtcttagg aatgccttgg ccggccattt
     1741 cgagatactt cgattccaaa gaagactggg tgctgaccga gttgtcgtcg tggcccaggc
     1801 tatagtaggt aaagacgccc tttcgagcat cgtcgatgag ccgataattg atgctcatat
     1861 tcttgcggcg ccgattgtac caatcctgca ggtattccat gggaatgcgt cgcatgatag
     1921 cgaaccaatc gttccgttcc tggggattga gcccttcttc atccatcttg gcctgaagag
     1981 aggctaattg atagatatcg gagcccatgg tcaggtagta gtccggttca acgcccttgc
     2041 cgccggccca gggactgcgc atgaccgtgc tgaatacgta gaggggcact ttggtattgg
     2101 ccttgtgcag ggcttctact ctttcaaggc gcgtcaagag ggtcgacatg ggaaggttgt
     2161 gcttccggga atcgacgaga ccgccgtaga ctaaagaatc aatggacagg accatggcat
     2221 cggctttatt cgcgttattc tctacccaag acatgagccg gtccggcgaa ccgtggaaat
     2281 tcatgcccga cagatactgt gcgggcggcg tcaaaatcgt atagcctgcc gattctgccg
     2341 tactgaccgt atattcgtaa tctacaggcc ggtcgtcctg cggtacgtat aaaatcgtct
     2401 tagccagtgt cgacggcgta gcacacagga acagggatac gacggccatg ctgactgcct
     2461 tctgtaacag attcacatta tcctcctatc gtaagaaaaa acaagtattt tttattatag
     2521 cacatctgcc ctctaaaaag aagaaccttg cttcttacgg cggatgcggg gcacattgag
     2581 gtggaagcga atggcaacca accggatggc aacggtaacc gaaaaactga cgatggcccc
     2641 cacttcaaaa ggcacttcaa agggaatgac ggcgccgtac aagaggacac tgccgaggag
     2701 cgacgccgta gcatagactt cagtccgcag gacgaccgga atccgtccgg ccaagacgtc
     2761 gcggatgacg ccgccgccga cggccgtgag gacaccgagg gtaatatcta aaatccagta
     2821 atcaggataa taataacagc cgagaagggt cccggttacg gtaaaggaac ccaggccaat
     2881 ggcatcacac aagaggtaga tggccgacag atgacccata aagcggcgcc acatgtggat
     2941 attgaagtag cggacagtca aggtgacgat aacgatggaa cctaatataa tccataggta
     3001 aatgctgttt ttaaaggccg ccggaggaat attgccgatg aggatgtcac ggacgatgcc
     3061 gccgccgaca gccgtcgctg cgctgaggac gaagatgccg aacagatcca tgcgacggga
     3121 aagggctgca aaaacgccgg atacggcaaa ggccgtcgtc cccaataagt caaaaatatg
     3181 ccaccaaatc gcatccatag gaattcccct tccctctctt tcgtgatatg ataaatgaac
     3241 catgttaatt actaaggaga tcatcatgac taaagatgaa ttttatatgg gcaaggccct
     3301 cgccgaagcc aaga
//
LOCUS       DAWDXZ010000149         3307 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6425_length_3307_cov_8.091021, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000149 DAWDXZ010000000
VERSION     DAWDXZ010000149.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6425_length_3307_cov_8.091021"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1040..1049
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          875 a          887 c          774 g          761 t
ORIGIN      
        1 ctgatagatg acattatcac cacaggtgca acagcggcgg cagcggtcaa gctgaccggc
       61 accatagttc tgcccgacaa aggtcgtgca ggcctggctg aaggaattaa gcagatcata
      121 cgtaatcatt tcaaggttaa aggctgcagc cgaagcagcg atggcattcg tccccaagct
      181 gttgatagcg gcctggataa cgatgttggc taaggtaaat acgccgcttt gaacgcctgc
      241 cggaagaccg atggcgataa ccgaacggac gcagtcccaa tgaatgccga tatcgttgac
      301 cttcagctga atgtaacggt ggggccgcat caacaggcgg aacaagacgc cggcactgac
      361 agcgttggcc atcaccgtag ccaaggcggc accggcgaca tgcaaatcga ggacggcaac
      421 aaaaaacaga ctgaggatta catttaccag tcccgatacg acgaggacct ttaaaggcat
      481 gcgcgtatcg ccgacgctgc gaaagataga cgattcaaaa ttgtacagca tgatgaccgg
      541 cagtcccaag agataaattc gtaaaaagag cactgccgac ggcaagacgt catcggggac
      601 attgagagaa ccgagaagcg gtgccgccac gacttctccg atgatggcca cggcgacgcc
      661 gatgaccagg cccatgacga cggccgtatg taccgtatgg gttacggtca gtctgtcctt
      721 acggccgatg gcattggcga tgacgacgtt tacaccaaga gccaatccga taaaaaggtt
      781 tagaattaag ccgataatgg ggatattagc cccaatgccg gcgacggcaa tcgtattggc
      841 ggcaccgctg aaatgaccga caacggccaa atcagctgcc agaaaaagtt gttccaaaat
      901 gaccgtggct gctacaggca aggcaaagag agggatttta tcccagagtg aaccgtgcag
      961 catgtcgatt ttaaacattt tttgatgtac gtgatgcatt gtatttgttt cttccatagt
     1021 aggtctcctc ctaacgacan nnnnnnnnna agaaaggaga ctctcatgga tattcgcgtc
     1081 ttacgttatt ttctggccac ggccagagaa caaaacatta cccgtgccgc tgaaaccctg
     1141 cacattgccc aaccctctct ttctaagcag ttgatggaac tggaagctga actgggaaaa
     1201 cagctcttaa tacggggaaa gcggcgcatc accctcacgg ccgacggaat cttgctgcga
     1261 aaacgagctg aagaaatcgt cgccctcctt gaaaagacgg agcaggaaat cgcttcaacg
     1321 gaccaggata ttactggcac cgtcgccatt ggcggtacac cgcgtcaagg cgtccttgaa
     1381 gcggcgtcaa cccttcatca gcagtatccg caaatccgtt tttccttcca tgtcggcgat
     1441 gcgcccgaca tcatcgaacg cctcgaccac ggcagcctgg atttcatcgt cctcctcgac
     1501 cggccggaca aggcaacgta tggatcgcat cccctgcccg atgtctccct ttggggcctg
     1561 ctcatgaaaa gcgacgatcc cctgacaaaa aaaacagccg tcactccaaa agacatctgc
     1621 cgccgcccct tggtcattgc ccaacgagaa gggattcgcc aaatgctctt cgattgggca
     1681 aagcccgttt ccgaccaatt ggacatcatc gccacctaca acgtcatgaa cgggaccccc
     1741 gtcccctttg ccctttatac cccgggcctg gtcctcatca tccgcgacct cctgtcgccg
     1801 accatcgacc cggccgtcgc ctttcgcccc ctcgatccgc ccataaaaac cagtcattac
     1861 ctggcctgga aacgctatcc tgccttttcg gcagccagcc agaaattctt agaaaccgtc
     1921 tatcgactct atccccaaaa atagcctgta aacgggccct gcccaaaaga aggagctgcg
     1981 caaaccatca aatattccta tgcgaaaaag acttatggat gagtgattca tggcaaatac
     2041 caacgacctt tccttttgtg ccaaaagaaa actcccttct aggtgaacag gatatactta
     2101 gttaccggtc cactaaaaag ggagtaattt ttttctatgt ctgatagtgc gctatatcag
     2161 agaatcgact tatagagatc tacaatttgg gacttttcga cgtctctcgg atttccgggc
     2221 gtgcaggcat cggccatggc cgaatcagct aagaaatcaa tatctttttc ctgaacgcca
     2281 aggtcggaaa gttttcgagg aataccgaca tcgtctgcta attgttggca ggcatcaatg
     2341 gcggctttac gatattcgtc ttgactcatt tcgtcaacgc cggcaacgcc cattgcccga
     2401 gcaacttcac gatatcgttc ccccgtggca gaagcgttga atgtcaaaac aggtgctaag
     2461 aggatggcac aggccacgcc gtgaggaata tcataaaaag cacttaacgg atgggccatg
     2521 gaatggtcga taccaaggcc gacgttcgaa aatcccatac cggcaatgta ttggccaagg
     2581 gccatttttt ctcgagcctc catatcccct tctacagatt tacgcagcca tttgctaatg
     2641 atctcaatgg ctttcaagtg catcatatcc gtcatttccc aagcattctt ggtaatatat
     2701 ccttcaatgg cgtgaactaa ggcatccatg ccggtagccg cacaaagttt ttttaggcat
     2761 ggatgccacc atatccggat caatgacggc tacgacagga atatcatggg gatcgcagca
     2821 gacaaattta cgatgctttt cagggtctgt aatgacatag ttaatcgtaa cttcagctgc
     2881 cgttccgctc gtcgttgaaa cggcaatgat cggcaagcac ttgttcttcg taggcgacgc
     2941 cccttcaaga cttctgacat cggcaaattc cggattcgtc aaaatgatgg cgattgcctt
     3001 tgctgtatcc atagctgctc cgccgccgac agcgacgata atatcggtct ttgcatcttt
     3061 acaaaaagct acgccgtcct gaacattttg aatcgtcgga ttgggcttaa tccgatcata
     3121 aaccgtatac tggatgccat ttgcatcgaa aagatcggta atctttgtcg ctactttaaa
     3181 tttcattaaa tccttgtcgg taatgaccgt aacatgctga cagccgcggt ttttaacttc
     3241 tgtgacgatt tcctgaatag cccccttacc aaagtatgat gtttcattta aagtaatctt
     3301 atttgcc
//
LOCUS       DAWDXZ010000150         3303 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6434_length_3303_cov_7.911330, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000150 DAWDXZ010000000
VERSION     DAWDXZ010000150.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6434_length_3303_cov_7.911330"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    380..435
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    753..762
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2489..2498
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          689 a          940 c          891 g          707 t
ORIGIN      
        1 ccggcacggc atcggccgtt tccatgggcg taaagccgta agggccgcgg tcttttcggc
       61 aggcgtcgcc ggcggcaccg tggagataga cgccggcaca ggcggcactg caaagatcgc
      121 tcatgccctg atgggccgtc atggcggcaa tcataccggt cagtatatcg cccataccgc
      181 cgcaggccat gccggcattg cccgtcgggt tgacgtagac gttgcccgtc ttggccgaca
      241 cgatgagggt cggcgcccct ttgaggacca ggttgacctt ccagtctgcc gcaaaggccc
      301 gagccgttcc gatgaggtcc tctttgatgt catccaggga caatccgctg aggcgggaaa
      361 attctgccaa gtggggcgtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnccctg tcccagggaa cggatgaatt ccttttcacc ggccaagagg
      481 ttcaaggcgt cggcgtcgag gacgacgggg cacgtcgttc cggctaagac gcgcttcaag
      541 aattcccgcg tccggtcatc cttccccatg ccgggaccca tggccaatac cgaccagttc
      601 cggctttctt cgatgatgga ctcggcgtcc tctgccgtaa aagagtcgcc ggtcccgaca
      661 cttttgacca tgacttccgg ctggacaccg atacagtaag gcccgacctt accgggtacg
      721 cggaggaaga ctttcccggc accgctgcgg aannnnnnnn nnccatttgg ctgctgccgg
      781 tgacgacggc gatcgtcccg tgactgccct tatggctgtc ggggctgcgg ggcacgacga
      841 tttccgcgat gtccgactgc tgcagtaaat agacggcatc gtcgttggca ccgtatagga
      901 gcggcccggg catgccgatg gaatcgactt cgatatggcc ggcgcaggcc cgtccgggat
      961 agagggaaag gcctcgtttc aagacaccga aggtgaccgt catgtcggca aagaggggtc
     1021 cgtcttcttc gtcgtcgcca tggctgacga cgcccgtgtc ggcattgacg ccggtcggca
     1081 tgtcgacggc gatgaccgtc accgccccga cggcagaggc cacgttgatt tccgctacga
     1141 tggctgctac aggttggcgc aggctgccgt gaaagcccgt tccgaccatg gcatcgatga
     1201 cgatggacga ggcgtggagc cgttttcgca agagctgcca atcggcttga ctttccggcg
     1261 ccgaaaggag gagacacgtt tcgtcatctg ccagctcccg taaggtatgc agatgctgcc
     1321 gcgcttcgtc gctgtaatca ccgtcatcgc ctaagatgta gacataggct ctggctccgg
     1381 ccgctaagat atagcgggcg atgacgaagc cgtcaccgcc gttattgccc ttaccgcaaa
     1441 agataacgac gtctttgccg tcccagcctt cgatccattt ttcggcccgt tcggcaacgg
     1501 cccgaccggc gttttccatg agtaccgccg gtgctatcgc gtatattccg tgcatggctt
     1561 cataatccat ttgccgcatc tgggcggctg tatttatttt tttcatggcc tggctccctt
     1621 ctaaatccta aatcgtatcg cccttacagg acgacgaaag cgacggcata gtccccgtca
     1681 tgagcaatgg acagggacgg cggctgacag cttttttccc gcacggcttc ggcatagaaa
     1741 ccgtaacagc aaaactgcgg cgccccccat tcatcgtgaa cgacttcgac gtcggtccat
     1801 tttccccgac gaaaacccgt tcccaaggcc ttgaacaagg cctctttagc cgcaaagatg
     1861 ccggccaggg actccatccg ccacgacccg ccacgctggc agtaggacag ttcctgttcc
     1921 gtaaagaatc gatgcagcga cgtgccgtga cgccgcacta aatcatccat gcggctgatt
     1981 tgtaccatat ccgttccgca gcgcataatt cctccaaaaa aagagccgca atcccctcag
     2041 gaactgcggc aacgttaact accacttctg cattcggata cagaccccca gcccttccag
     2101 caagacccga aaggtcgtaa aggacacggt catatcatcg tgaaggccgt gggctacttt
     2161 ataagaaatc gtaacagttt gatgctggta atcgtaccga aagccatgta aattcgtttc
     2221 aaaggtatat ttcggtgccg gaatggaaaa cttcggcggc ttctcgactt ccagcccgtg
     2281 aatgagccgc tccaaggtca ctcgcttggg cagttcagac tgcagcattt cttcggtaaa
     2341 gctatccaat acagacatgg atccccctag tcttcttcgt cgaagaagtg ctgatgaatg
     2401 gctttgaccg ccgaatcgac gacttcttcc gcaatgaggc aggtaatact gatttccgac
     2461 gtagaaataa tttcgatgtt gatgtcatnn nnnnnnnntt gtcgctgagg acgccgaaca
     2521 tgccggcagc aatgcccggg tgaccggcca taccggcgcc gacgacggaa accttagcca
     2581 tcttgtcgtc gatgatgatg tcttcaaccg gcatttccgt gcgcagggca tggaagaccg
     2641 attcggcttc ttccaaatcc gtgcgggcaa tggtaaaggt aatatccgta acgccttcgt
     2701 tggcatagcg gacgctctga acgatcatgt cgacgtcgac gttcttttct gccaattcag
     2761 aaaagacgcg ataagcaaac cccggtttat tgggaatccc gagaacggtg attttagcaa
     2821 catttttatc cgaagcgacg ccgcggatga gatattgttt gacttccata gagcaatcct
     2881 cctgtatcat agttccttca tcttccgaaa aagtcgagcg cacgtaaatg ggaacgccga
     2941 agcggctgcc catttcgacg gcccggggct gcatgacacc ggcgccgagt ttcgccattt
     3001 ccagcatttc gccgtatgtg atctgatcga tttttctagc attgggaacg acacggggat
     3061 cggacgtgta tacgccgtca acgtcggtat agatttcaca aacatcagcg tggatagcgc
     3121 cggcaatggc gacggctgtc gtatcggaac cgcctcggcc gagggttgtg atgtcaccat
     3181 gaccggtaat gccctggaaa ccggcaacga cgacgatatt atcttgttcc agtgccttca
     3241 tgatacgggc cggcttcaaa gccagaatac ggcccttgtt gaaagcatcg ctggtcttga
     3301 tgc
//
LOCUS       DAWDXZ010000151         3286 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6459_length_3286_cov_8.330857, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000151 DAWDXZ010000000
VERSION     DAWDXZ010000151.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6459_length_3286_cov_8.330857"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1716..1725
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          738 a          980 c          763 g          795 t
ORIGIN      
        1 tagtaatgcg tgtacctgcc atagcaataa tcgtagccgc tgatgcggct atcccatcaa
       61 cgacaacatt aacattgccc gaataggcaa ctagctgatt atggatagcg tgcgccacaa
      121 acacatcgcc gccttgcgaa ttaatatgga cagtaacgtt ttttccgcct aaatcttgca
      181 attcgcgcga aaatttagcc gcatccacag aatcggacca tagcggggct tcagccacga
      241 tatccccgta gatatataat tcagcgttat cgtctcctgc ggcgttctga aaatcccaaa
      301 attcattcat cctgtatcac ctcccccggc accctgtgcc tcggtgttat cggccctcgg
      361 atacgccata ccattcttgg cccaggcgtc gcgctccagt gctatctggt cgacgttatc
      421 gtcgtagctg gtcccggtaa gctcggcggc ctcgcgttca gccgtcgaga atccatactt
      481 aacgcgtaat gccgcgccgt taacctcttt aaccggatca agcaagccca taaccggacc
      541 ataccagctg gcgccggtcc atgcctgggt aatcaagggg tcagccccaa agccaggcgc
      601 atcgatacgc ccgatagcta ccgcttccgc taaccacgcc tcatagacgg gctggcagaa
      661 gtcccgtgca aaccatgtac gtcgagtacg gaacatcgtc gacgcctgca acagcgccgc
      721 tctagccgca ctgtacgacg actggaaacg attcatcaac acttccgacg gaatccccag
      781 ggacgcgcca atctgcgcaa tcagggaatt agtgaattct tcaaacgtcg actgcgtccg
      841 actgccgtca atggccttaa cgtcaacgcc actgggcagc agattcagcg ttcccggccc
      901 gacctcgata tgccgcaggt cgtccgggtc aatctgttcc gccggcgcgt atgtctcgct
      961 gagtacgtcg ccaaggtcgc ttgttgttcc cgaagttgtg aaaaatagtg taaaaaacga
     1021 cttgataatt gccgcggata gttccgcgtt ggtgtatctg cttacctgct taagcacttc
     1081 gatagccggc gccagatacg ggacaccgcg atactgttcc gggcgctcct catgagacac
     1141 ttgcaaaata agcggcatcc cggtacgcct gccgaaagcc tctacccggg tccactcaag
     1201 ccgctgagag ttgttagtcg ggtcataggg tacccggtta gctacccaat acgcgactac
     1261 cgctccatcc gcgtcaatct cgacaccgtt aaggatgcgg ttattgttga ccgaattacg
     1321 gctctcgacg gtcatgttat ccgtgttact gtaagcagcc atatcattcg gattgcacac
     1381 cctcgacgcc tcgaatagtt gtatcttagt cgagtagata ctgcccatcg tcggcttgcg
     1441 atacttgata gccgcccagg cgtcgccgtc caccagatac gacagatagg cgatatcctg
     1501 catgtcatag aacgaatgtt ttctgtacaa gtcgcaatgc accgagtccg cccaaaggct
     1561 gaactcctgc tgtgtacgat gctgccaagc cttagcctca tcagcggtca tccccagtag
     1621 cttataatca atcttaggga ctaccctgag ccctgcaccg atgacgttag cccggttagt
     1681 attaacagca ctcgcgccga tcggagagtt gatatnnnnn nnnnngcgat gacttcaggg
     1741 gattgtaccc tcgcagcgtc gaccgggacc gactagcacc gccttcgctg tatccggtat
     1801 ttgtaatttt agtatccagg ctacccgtag gatgccgtac tttacgtttt ttcctgctca
     1861 taacgacctc ctaatcgcgt aatattactt gcttagtcct ggacgcattc cgactggctc
     1921 cgtcgatact tgcaccagcg gccagtaatt tgctgatctc gctccgaatt tccgccaaat
     1981 cggcgcgagt caatgtacga ttgccaatct tatacgactg cccaccggtt aatatggcag
     2041 tttctgcggc catatactgc ttcagccgca tgtttaacac atcgctcatg ttatccctcg
     2101 cttaatcacg ccaaaattag cctttttagg cttttgcggt ctgcgttttt cgcctgccgt
     2161 ttcgcccgtc atcagggctt taagccgcga aaaatcaggc gcaatcgatg ccaagcaagc
     2221 cagattgtat actctcaggt caagcggctc atttcgatgg tccttagcca cgttgaccca
     2281 acgatataca atacttccgt tgcgtttctg gggctgtttt tcttccgctg taaggccttg
     2341 gaaatagatt tcatcgtagc cgcgattcca caatatttca ttaaccgcat cctgttcaga
     2401 tttatcgagc ggaaaatgga aataccgcgg ccccggtacg tcaatggcta agcgatccat
     2461 gacatactgc ttaccgctat cagtcccaat gaggaccagc ggaattgtac gcccgcgata
     2521 agcttgagcc ttagagtact tgtgtatgat aggcacgccc atcgtcgacg acccacgcac
     2581 cgcaaaccgc tgccgggcta atcgtaataa gctataccgg tacacctcgc tagtgtagtg
     2641 gccgccggag tcgataaatg ttctggcgac cagaaggccc gtcccatcgg cgaagtgata
     2701 ttcacggtca agttgctcat cgagttgccg ccacacggca ggcgtatccg gagcgcctag
     2761 gataatgccc ttcttaattc cccagcattc ttcttcctcg ccccatccga ctatttcata
     2821 ctccaggcga ttatcctgga cgtcgaccgc cgccgtcaaa atcagcacgc catcaggcag
     2881 ctccgcatta tacggctctc gacgggccat cagcttatcg atgtcatcat agctgcgagt
     2941 acgttcatag ggttccccaa atcgcgtatt gacgactacc ttctcgcgct ccgggtctcc
     3001 tttggcgtca acccactcct gcatgacgtc agcccaggaa atccaaggcg acgcccagca
     3061 gttaacaaaa aaactgcgta cgtgattacg tatcgcagta ggattcttgg caacgtactt
     3121 ctgcggctgc cgccgcatct cgttttccga aaacgtaaat ccgcagtccg ggcatcgcca
     3181 cgacaccgac ttgacatgcg tatgttcttt ttttcgaccg tccttgacgg tctccgtatc
     3241 cattaccatg ctccgatgtg tcaacagatg atattcgccg cagttc
//
LOCUS       DAWDXZ010000152         3272 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6478_length_3272_cov_5.967983, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000152 DAWDXZ010000000
VERSION     DAWDXZ010000152.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6478_length_3272_cov_5.967983"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2815..2824
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          867 a          649 c          896 g          850 t
ORIGIN      
        1 tgagtttttt gcaaatacgg aatagccggc ggattacttt agaatccggt gccgttaagt
       61 acgggctttc ttcgtcgaag gagtgatatg gttgagctgc ttattttcgg aggggagacg
      121 attctacgct gcgtaggtag tattattgcc ccttttattt taagataatc atagtaacgg
      181 aaagaggtgt aatgtatgaa ttatatatca ggaacgatgt tgaaagaact gcgtgaaaaa
      241 aagaagctta cgcaaaagga tttagcggag aaactgcgta tcagcgacaa gacgatttcg
      301 aaatgggaga ccggcaaggg cctgccggat attaccttgg tagccccctt agcggaagcc
      361 ctcggtattt cggtggcaga actctttgcc ggtgaatatg ccgtcaatga taaccgctcg
      421 gccaatgtga aaaaacttaa gttttacgcc tgtcccattt gcggcaatat catcaccacc
      481 ttcggcgaag gcgattataa ttgctgcggc gtcaaattgc cggtccttac ggtggaagac
      541 gcttcttcag ctcatcaaat caattatgac atgatcgaac atgagttttt cgtccatatc
      601 gatcatccca tgactaagga gcactatatt tcctttatgg cctatgtgac ggccgaccgc
      661 tataccttgg tcaagctcta tcctgaacag gatgcgcagt gccggttcat gagccggggc
      721 catggcttta tctatgccta ctgcaatcgc gacggcttgt ttaagataca tgtctagtga
      781 aattgttttt tctgtccttg acctcctgag ggttagccct aaaaagttaa ccctaaggag
      841 acctttgttt aggcaaattt gtacgtatat ttacgactct gtgttatttg tgttatgata
      901 aaagcaataa aagttatgat acgtatttgt ttttatcatg gaggtaacga tgagtaaact
      961 ggatttattt aaagagcatc tggatgaaag agaatatcgc tatcgtgagg tcaccgataa
     1021 ggagggcgtg acgcgggggt ttattgctca acacgagatt aaagacaatc agttacttac
     1081 ggtggttttt gaattcccag aggaagaaaa ttttgtagac gtgagaattt ttgatatcgc
     1141 caagatagaa gattcttcaa aacgagaaaa tttattggaa ttgattaatg aactcaatct
     1201 aaagtatcga tttggcaagt ttttcgtcag agatacaggc gatgttgtca tgcagtggtc
     1261 attgtttatt aaggatcact ttgatcccgc atttcttctg gatatgactt ttatactcgt
     1321 aggcaatgta ggcgaagagt atcctaagtt tatgaaactt caatggacat aaagataaca
     1381 tgcccttctg aataggaagt tcatattcaa tctattgttc catcgagaca gattgtttta
     1441 gatgactgtc tcattaaatt ggttttacca ttcttgcccg tctcctgagc gggacggagg
     1501 aacggtatgc ctaggcctaa gaggccctgc agtatatctt cgatcatatt tgtaagtaaa
     1561 aatgggattt atcatacctg ttgaattgac aaagagggca cgacggagct ttgggcttag
     1621 gtcgtgcctc gctgttttta acggcttttc ttgaagattc ggtaaggccc tgctcctacg
     1681 ggcaggcgtt ctttgtcata taaagtataa tatatgatat aataaagtaa atctaacata
     1741 tatacaaaag atggccgatg tgagcctgtc ggttggaagg agcaagacga tgaagaagaa
     1801 atgcagttgt tgcgggaaga aaaagaaggt tgtccgtttt gacgggaagg tatggtgcaa
     1861 gtcctgtgtt tggcacgaac tgaaagaacg cagtgctgcg gaagtggaag cagacgccgt
     1921 cgaagaattg aaagcgcccg acaccatgga aaacaggact gccgccgagg aactgcagga
     1981 catgaaggaa gctgtaaagg gattggctac caagcagaat ctgattaaag cgggcacttg
     2041 tgccgtcggt gtttttgtcg tcggcatgat tgccgtccat tttggcgctg accagatggt
     2101 ctatcgttgg ctgaatcctt gggcaccgtt ctaaattgaa cgccggtcat cgtgagatgg
     2161 tcggcatttt ttttgccaga aaatagggag gagcgtattt ctttatagga ataaaaaaag
     2221 tcccaccatg gtccgcagtg cgtttccgcc ttatacgtgg tgggctctat cgactatagt
     2281 gtatcaaact ttaagaaaaa aacaaggggt gcagtcgtat aggaacagcg ctagcctgat
     2341 tcggacaagg cgggattctt ttgatgtaat ttaaatagtg agtagccata aaaaatgacc
     2401 tcccggcgtt cgtctttcaa aacggacgtt aggaggccat ttcctttgct gttaaggctg
     2461 cacgttaagc ctgtttttta atgcggcagg cgacgcagta gatgacggcg cacaagccga
     2521 gataggccag gatgccgagg taggtggcac ccggtgtcgt gacccagtct tttaagaacc
     2581 agtcgacgcc gccgatgtgg aggacggcac tgatgaggct gccgatagca ccgccggcga
     2641 cgaagcccga ggcgatggtg ctgccttcac tgagtcggag ttcatttaag gcttcgtctt
     2701 tgctgctgtg agagacgaag tacgatacca agccgccgat gaggaccggc gtgttgattt
     2761 ccatgggaag gtacgcgccg agggcgaagg ccaacggcgg aactttcatc atccnnnnnn
     2821 nnnncatggc attggcctga ggagcggcta aggcaccggg gccggtgtag ccgtaggcgt
     2881 ggttcaagag gaagatgaca ccgacagaga cgaagctggt gacgatgatg gagacgattt
     2941 gccactgctg catcttcttc ggcgtggcgc ccgtgaggta ggcgactttc agttccgaca
     3001 tgaagttgcc ggcaacggcc aaggtggcgc agaggaaggc ggccatcatg aggatggcga
     3061 tgatgccgga actgccgctc atgccggcgt tgcccatgag gacggccgat acgatgatca
     3121 taaagatggt catgcccgag accggttcat tccccgtaaa ggcgatggaa ctgatgccga
     3181 cgacggagag catgaaggaa aagatgatga tgatggcacc ggccagaagg gtctgggtga
     3241 tgttctggga gcaggtgaca tggaagaaga gg
//
LOCUS       DAWDXZ010000153         3265 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6490_length_3265_cov_5.587227, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000153 DAWDXZ010000000
VERSION     DAWDXZ010000153.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6490_length_3265_cov_5.587227"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    473..482
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          893 a          746 c          850 g          766 t
ORIGIN      
        1 gtgtaaggac cttgaccgca atcccgagag cgtcggcatc gatacctggg gcgttgattt
       61 tgtcctcctc gataaggccg gccaggtcat cggcgatacg gttgcttatc gcgacggacg
      121 tacaaagggc atggatgagg aagtttataa gacgatttcc gaagcggaac tttacagtcg
      181 caatggcatt cagaaaatga tgattaacac catctatcat ttaatggcca tcaaaaaaga
      241 aacaccggaa atactggaca aggcagaacg ttttctgatg atcccggaat atcttaatta
      301 tcgactgacc gggattgcca tgaatgaata cactaattca acaacatcac aactgattaa
      361 tgcaaaaaca caggattggg attttgaact ccttaaaatg ctgggcctgc cgacaaaaat
      421 ttttggtgac atccatatgc cgaagacgct ggtcggcccc ctttcaaaag aannnnnnnn
      481 nngaagcgtg cgtcggattc aagacccatg ttgtccttcc ggccacacac gatacgggtt
      541 cggcagtcat ggcagtcccg acgaattccg atgacagcat ttatctgagt tcgggaacct
      601 ggtcgctcat gggaattgaa cgcctgattc cggattgtac cgaaatgagt cgaaagcata
      661 attttacgaa tgaaggcgga taccattatc gctatcgcta tctcaagaat atcatgggaa
      721 tgtggatgat gcagaatctg cgccgcgagt ttaagcatcc gtacaccttt gacgaacttt
      781 atgaactcgc ccatatcggc cgctatttta catcgacagt tgatgtcaac gacagcgctt
      841 tcctggcccc ggacagtatg atcaaagcct tgcaggatta ttgtgaaaga acgaatcagg
      901 aaaaaccgga aacggaatgt gaactcctgt attgtgtcta tcggagtttg gcaaactgct
      961 atgccaagac cgttgccgaa atcgaggaag tcacgggccg tacgtacgat acgattcatg
     1021 ttgtcggcgg cggttgtcaa gataaattcc tggacaggct gcttgccgag gcgacaggca
     1081 aaacggtgta tgtcgggccg gtagaagcga cggctatcgg caatatcatg gcgcagatgc
     1141 tgggtgctaa ggtctttgaa gatttaaagg aagcacggca ggtcgtagcc aagtccttcg
     1201 atgtgaagcg catcgaaaat caattcgagt aattccggtt ccataaccgg agcgattaag
     1261 atgttggaag ttataaatat tatatatcag gtgatgtacg tcacctcaag gttacaggat
     1321 ttataggagg ttttcattat gtcgagattt gaagaagcaa aagaaagtta cgcatcgata
     1381 ggtgtggatg tagaaaaggc cttacagaaa ctgcaagatg tacgtatttc catgcattgc
     1441 tggcagggcg acgatgtctt aggattcgat tcggactcac tgactggagg cattgcggcg
     1501 acgggcaatt atccgggccg tgcacgcaat ccggaagaac tcatgcagga tatcaaaaag
     1561 gcttattctc tgattccggc taagcacaaa ctgaaccttc atgcttcgta ccggatcacc
     1621 ggtgaaaata ttgatcgtga tcagatcgag ccgaaacatt tcaaggcatg ggttgatttt
     1681 gcgaaagaag aaggcatcgg ccttgatttc aacccgacct tcttcagtca ccggaatgtt
     1741 aaaaacaaca tgaccctttc cagcccggat aaggatattc gtgatttctg gattgctcat
     1801 ggcaaagcct gccggaagat tgccaactac tttggtgaag aacttggcat cacggctctg
     1861 gacgacatct ggattcccga tgggacaaag gatgtgcctg ctgaccgttt gggacctcgt
     1921 cagcgcctca aggaatcttt ggatgaaatc ctttccgtga aatatgaccg taagaacatc
     1981 gccgacgcgg tggaaggaaa ggttttcggt atcggcgttg agtcgtacac cgtaggctct
     2041 catgaatttt atatgaatta cgccgccaag aatggcatca tgtgtctcct ggatacgggt
     2101 cattaccatc cgacagaagt cgtttcggat aagatttctt cgatgctgct ctttaacgac
     2161 agtttagccc ttcatgtgag ccgcccggtc cgctgggaca gcgatcatgt tatcaagttc
     2221 gatgatgagc tcaaggaact ggcaacggag ctcgtccgct gtaatgccct cgacaaggtg
     2281 ttcatcggcc ttgacttctt cgacgccagc atcaaccgtg ttgcagcctg ggttatcggc
     2341 atgcgcaaca tgcagaaagc tttgctctac ggcctcttga caccgaacaa agaactcaag
     2401 gaagcacaag atgaagccga tttcacgcgg gtcctgcagg gactggaaga aatgaagacg
     2461 ctgcccttcg gcgatgtctg ggctgagttc tgcaaacgca acgaggtccc cgttgacggt
     2521 cggtggtact ccgtcattac ggattacgaa aaggacgttc tgtccaagcg ataagagaaa
     2581 ctataatttg aaataaagaa ggagtcaatc atggatatca aagattcaac atttatggaa
     2641 ggttttaagc gcctgacggc agatgcattt cagaaaggct ggcatgaacg aaacggcggc
     2701 aatttgacgt atcgtgtcaa aacagaagaa gtcgacgccg tcaaggaatc gctgcacgac
     2761 gtgaaagaat ggcaacctat cggtaatacc gttcccagcc ttgccgggga atatttcctg
     2821 gttacgggaa gcggcaaata tatgcgcaac gtctcattgg caccggaaga aaacacggcc
     2881 ctcataaaga tcgacgaaaa aggcgagaat tatggcattg tttggggcct ggtccatgga
     2941 gggcggccta ccagtgaact gccgacacat ttggcagccc atgaactgaa gaaaaagatg
     3001 accaacggga cgaaccgcat catttatcac gcacacccga cgaatctcat tgccctgacc
     3061 tttgtcctgc ccctgaagga taaggaattc acgcgtgagc tttgggaaat ggccacggaa
     3121 gatcccgtca tcttcccgga aggtatcggt gtcgtgccct ggatggtacc gggcggcaag
     3181 gaaattgccg acgcctcggt taaactgatg gagaaatacc gcgtcgtcgt ctgggcccat
     3241 catggcctgt tcgtttgcgg cgata
//
LOCUS       DAWDXZ010000154         3254 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6519_length_3254_cov_7.882463, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000154 DAWDXZ010000000
VERSION     DAWDXZ010000154.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6519_length_3254_cov_7.882463"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2499..2508
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          767 a          824 c          864 g          789 t
ORIGIN      
        1 tatacggata ccgtctttaa ggagtatgtt gacggagccg tgtacgatat catcgacggc
       61 ggccgctatg accgcctgtc gtctcgtttt ggcgtcgatc gtccggcctg cggcttcggc
      121 atgaatatca acctcttgta tgaatacatg aacgatgccg gtattcttga agatacggag
      181 ccgtcttttc agttagccgt ttcctatacg acgagtgatc agccgttgat tcgggatctt
      241 atgaattggc gcagtagagg gttccgcgtt gcggcctatc ctgattcgtc atttatcgat
      301 agttctgatt acagcattca tgccatatac cgcaacggct cgtattataa agacggtcag
      361 tgcctgacgg cagaagacct ggaaacattg atggggaggt tgtaagatgg ccattcacat
      421 tgccctggct aagggccgca ttcataaagc cgtcatgaaa tatctggccg acagcggcta
      481 tgaatttccg acgtattccg aagcaagtcg ccggctgatt tttgaagatg ccaaaggaca
      541 gctcaaggtg actttagtca aatcgcccga tgtggcggtc tatgtcgaac gaggggcggc
      601 cgatgtcggc atcgtcggca aagacgttct ccttgaagaa gctccgggag cggatgtcta
      661 tgaagtcatg gacctcggca tcggctgctg ccgcatggcc gtcgccggca ttgccgggaa
      721 aaaagttgac atgaccaagc gaattacgat tggtactaag tacccgcgga ttgcgcgcag
      781 ttattttgaa gccaatcatc acagtgtaga tattatcaaa ctcaatggct ctgtcgaatt
      841 ggcacctatt gtcggtttgt ctgatgtcat cgtcgatatt gttgaaacgg gcaatacgct
      901 gaaagcgaat ggattggaag tcttgaatta tttcatggat ttcagtgccc gcatgatctg
      961 caaccccgtt tcgtttaaaa tgaagtaccg cgatattcag ctgctcatgg acgtcatccg
     1021 tcagcacagc ataaaggcat agtaggggga atcaggatgg aatggataga tgtacccgcc
     1081 aaggggttga ccttgcgcga tattcgccgt attactcggc gcgatcaggc cgaaagtgaa
     1141 gatatccgca aggctgtaga agaaatttta gccaatgtcg aacaggacgg agacgaagct
     1201 gttcgtatgt atacaaaaaa atttgacggt gtcgccatcg aggatttcct ggtcacgccg
     1261 gaagaaatag accgggccgt tgctgccgtc ggtcccgatt ttatgagtat ccttgaagaa
     1321 ggggcggcta acatccgagc ctatcatgaa cggcaggtgg aaaagacctg gttcgatacc
     1381 ttccgtcccg gcgtccgcct gggtgcgaaa ttcacaccta ttcagcgcgt cggcgtctat
     1441 gtgcccggcg ggacagctgc ttatccgtca acggttctca tggatacgat tccggctcat
     1501 gtcgccggtg tcccgtccat tgccgtcttt accccgccgg ctaaagacgg atcggtcaat
     1561 ccgtatattt tggcggcagc tcacgtcgcc ggtgctacgg aaatttacaa ggtcggcggg
     1621 gctcagggga ttgccgctgc tgcgtacggt acggaaagca ttgcaccggt ctttaaaatc
     1681 gtcggcccgg gcaatgccta tgtagccatg gcgaaacggt tggtcttcgg tacggtcggt
     1741 atcgacatga ttgccggacc cagtgaagtc ggcgtcttgg cagaccacga agccaatccc
     1801 gtatggattg cggccgacct attggcccaa gctgaacacg atcggcgggc tgctgtattc
     1861 ctggtcacgc cgagtgcagc ccttgccagt gaagtagaac gcgaaatcaa acgtcagttg
     1921 gaagacctgc cgcgtaaggc tattgcttcg gaagcgattg aaaaatacgc caagtttttt
     1981 atcgttaagg ataaagatca ggccgtcgac atcatgaacc tcattgctcc ggaacatttg
     2041 gaaatcgcct tccctgatgc cgaagactat gcgaaaaaaa tcgtcaacgc cggggccatc
     2101 tttatcgggt cctatacgac agaaccttta ggcgattact ttgcagggcc taatcacacc
     2161 ttgccgacta tgggaacggc tgctttttca tctccccttg gcgtctatga ttttgttaag
     2221 cgcagcagtc tcttgtccta taataaggaa gcttttgaag ccgtagccga taaggtcatc
     2281 cgctttgcca atgtcgaagg attacaggct cacggccggg ctgtaaggag gagaatcgat
     2341 gaacaataaa tggcttcgtc agaccataca atcttttaag ccctatgcgg caccgaaaat
     2401 aaacgaatca atcgtcattg acgccaatga aagtccttat aatatcttcg attttcccca
     2461 ggtcgcagaa gccttcctgg cacgcatgaa acagacgcnn nnnnnnnnga cccctttgcc
     2521 ggggaactgc gagaagcctt ggctcaatac gtcggctgtg gtccggaaga agtcctggtc
     2581 ggaaatggcg gggatgaaat catcagcctg gtcatgaaca ccttcttaaa tcccggagat
     2641 gtcctgctta cgcacacgcc gaccttcgat atatacggtc tcgatgccga agttctcgga
     2701 gctaaggtag tcactgttcc cgacctcgac ggctatcgta gagattgtga cggattcctt
     2761 gaaatggtaa aggaactgca gcccaaggtg accgttctct gcaatcccaa caatccgacg
     2821 ggggagctgc tgccaatggc gtatgtagaa gccgtgctac aggcttcgga aaaccccgtc
     2881 gtcatcgatg aagcctactt ggaatttgcc ggtgtgccca gcatcattac caagctttcc
     2941 caatacgaca acctcatcgt tatccggacc ttgtccaaag ctttcgggtt ggccggctgc
     3001 cgtgtcggtt acggcgtggc tcaaaaatcc gtcatcgagg ccttggggct gacgaaactt
     3061 gtctataacc tgaatgtcct ctctcagaat gcggccttgg cggctttaga atacgccgat
     3121 gacattttgg ctcataatat cccgccgacg ctggcggccc gcgacgtctt ctataaagct
     3181 ttagaggctg tgcctgaaat cacagcctat cccagtgcga ccaacttcgt cctcattcgc
     3241 gttcccgacg gacc
//
LOCUS       DAWDXZ010000155         3252 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6521_length_3252_cov_7.598999, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000155 DAWDXZ010000000
VERSION     DAWDXZ010000155.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6521_length_3252_cov_7.598999"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1767..1776
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2849..2858
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          978 a          833 c          520 g          901 t
ORIGIN      
        1 tagatttaga gcatgttagt attctcaatc ccaaattaga atcctgttct gctgaatagc
       61 aagattcttt tagataaaaa attcactgcc tctaaaaact atatcgaaag tcgaccttag
      121 acaataaacg ctgtatatta actaatccat atgctggccg ccattgatat cgatttcttc
      181 accagtaata taggaggctt ccttagatgc caagaatacg atggtatcag ccacttcttg
      241 agtttctccc gggcgcccta caggaatgcc atcaatgatt ttctgcgcct gttcctgggg
      301 caaagatttc cagatttcag tattaatgag gccggggcaa acacaattga tagtaatccc
      361 atatttgcaa atctcacgag acaagttttt agaaaaagct aatacccctg ctttagatgc
      421 cgaatagtgg gcgcccccaa aaattccacc gccccgctta gccgaaacag aagaaagatt
      481 tacaatgcga ccataaccgg ccgaaatcat aggtttcatg caagcttgag tgcacaggaa
      541 aaggccaaac atattgacat taaaaatccg cttcatatca tctaacgtca tttcagctac
      601 agtaaccttc tgagagatac cggcattatt aatgaggaca tctatccgcc cgtatctttg
      661 cataactcga gcaaccatgt tttcaaccga ttccttactc gtcacgtcga gtaaaattcc
      721 ttcagctttt ccgccttcat cctgaataat ttgaacggtt tcgtcaaccc cactctggtt
      781 aatatcagca ataatcacgg tgccgccttg ctttgcaaat gtttttgcaa taactcttcc
      841 gattcctttg ggagatccgg ctccagtaac aacaatcact tgatctgtaa aatctaacat
      901 aataataccc tcctcaatac atgactcttt tatgcaaaag catctcatac aacattttat
      961 tacaacacct taatggtacg tagttgatat tccttgttca ttgttgtgca aaacgggttc
     1021 agcttgcttc acgtcctgat tgtgaccatt ttcaacctca cgcaacgctt ttccggtttg
     1081 tttaacaaaa acagtaataa caacagcact gacaaccata catgcagcca agaacaacat
     1141 accagcgtta aacaccccgg taacggtact aatatagccc attacataag ggcccataaa
     1201 tccacccaag ttccccacac tgttaataaa ggcaattcca gccgctgcag ccggtccaga
     1261 taagtatgcc ggcggaatgg cccaaaacgg cgaatatgcc ccatatgctc cagccaaggc
     1321 aacagcatat actaataatc ccaagacaac atttacctgt gctgcgtaca gacttccaac
     1381 catccctatc gctgatacca ccaagcaaac cgccacatga tagcgccgtt cattagtttt
     1441 atccgaatgc aacccattta aatacataat tatggctgca aataagtaca tcacaccaat
     1501 tacccagcca atgacagtca tgctaagaga ctttgacatc tcggttacca gagttggcaa
     1561 gaacattaca attccataat agccgcacat ccagctaaaa taaccgactg acaagactaa
     1621 gacatctcgt tcttttaaag cggctaaaaa ggtcattgac tttttcgacg cttttaactt
     1681 ttgttcttca gaaacaacac caataagcca ttctttttca tcggactcta accaactcgc
     1741 atcttcgatt ttatcatcca agaaaannnn nnnnnncccc caggaaacca taattcgaga
     1801 aatccaagcc cgagcgccta ccttggtcat taaggctccg cccggaactt ctaataaaaa
     1861 atatcccaag aaaaagattc ctgctccaaa tccaaaaaca gcgctggaaa agcccaaatc
     1921 agcattcatc tgtaaggcag caaacccgat atttacccga tcaagaattg aaatagcaaa
     1981 acatagaaac aaaaagggaa tgagtcgtct tgttactttc tttacggtac gtctttcaat
     2041 cgcgctagta tccataataa ctcctttaaa aagcatcaaa gatcaaagct tttagccgtt
     2101 gccacaagat tttcaacggt aatgccgttt ttagctaaca gccgttcata cggtgcagat
     2161 tctccaaatt tatcacggat accgattcgt ttaacctgtc ctttccccat ttctgccgcc
     2221 acttcacaaa cggcacttcc taatccgtta agaatattat ggtcttctac ggtgataatt
     2281 tttccgatat tttctatgca gtcgcttaca gcctctacat caaggggctt tattgtatgc
     2341 atatccagaa ctttcacgct aatcccttct ccagccaatg cttcagctgc ttggatcgca
     2401 agatgtaccg tatcgccatt ggcaataatc gcaacatcgc tgccatcttt aactaaatga
     2461 gctttgccaa ttttaaactc tgcgctctca tcataaataa ccggtatcgc atcacgggta
     2521 aatcgaatat agcaaggacc atacatatct gcggcagccg cagtcagttt ctttgcggaa
     2581 acatagtctg ccggcattat aaccgtcata ttcgggattg tccgcaatac ccccatatcc
     2641 tcaatagcct gatgacttgc accatcatta gccggtgtta ccccaccatg cgagcaggca
     2701 attttaacat ttaagtttgg atagcaaatc tcctgccgta tcatttcaca cattcttaaa
     2761 gatccaaaga ctgcatacgt cacaacaaaa ggagtcttac cggtcgtcgc cagtcccgct
     2821 gcaacaccag ctgcattctg ctccgcaann nnnnnnnncg gtgccacacc atgccactta
     2881 cagctatttt ccatatacga gacacccttc cctttaaccg tatgggcaaa aatacaatgt
     2941 ggttttttat tttttctcat gcgtatgata tccaacgtat gaacaatttg ttccatgtta
     3001 ttaccatcaa tttcatatac atcaaaccca aaagattcaa atttttttcc aaggtcgata
     3061 ttaggcatga cttcatccgt tgttccatcg atttgaaggt tattattatc aataaataca
     3121 atcaaattat ctaattcata cttattggca gtttggcagg cttcccaaat ttccccttct
     3181 tggcattccc catcgccgac tgcaacaaaa acccgatagt ctttattatc attcttgccg
     3241 gcaatggcca tt
//
LOCUS       DAWDXZ010000156         3233 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6550_length_3233_cov_10.231907, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000156 DAWDXZ010000000
VERSION     DAWDXZ010000156.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6550_length_3233_cov_10.231907"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          783 a          930 c          851 g          669 t
ORIGIN      
        1 ccgccttggc gagacgcctt ttgccctggc tgaagtgacg gcctgggatg aaaccaagat
       61 gattccggcc agcgtcttaa atgaccttcg ccgtcaggct gcagatgcgc tgacggaagt
      121 tattttggct gattatcacc ggccggcggc aggtacggtg atgccgatat cggaaacggt
      181 cgttgaggta aaaaaaggcg aggtcatgga actcgtcgtc cgctgcgata ccgtcgacgg
      241 tgttgcggca gcggccgaaa agggagcaga cgtcattatc ttcggcggcg aatcgtacca
      301 ccatcgtcct ttcctccaga aggattggca ggcagccgtc aaggcagctc atgattccgg
      361 tgcggcctta tgggccggaa cgccgcgcat cgttcgtgaa gcccatagag cggccgtaca
      421 aggcgaactc gaacgggctg ctgcatccgg catcgacggc atttacgtcg gcgccatggc
      481 tgtctttacg atggctaaaa aggtcggcag cggccttcct atccgaggag actggtctct
      541 taatatcttc aacagtcagg ctgcctcagc ctatgcgtcc ctcggctgca gcggtatcac
      601 ggcgtctctt gaagcgacgc tgcgacagat ccgtgacatg gcccgcaaga gtccttgtcc
      661 tgttgaagcc gtcgttgaag gccgtgtaga aatgatggtc accgaatcgt gtgtcatctc
      721 atcctttgcc ggcaatggca aaaagaaggg ctgtcccaat gtctgtcagg gtggagccta
      781 tgccctgcgc gaccgccggg atgaagactt ccccgtcgct accgaccagt attgccgcaa
      841 tcatatccta aacagccgtg acctcgacat ggctccctac tataaagacc tcgtccgggc
      901 cggcattgcc gccattcgca tcgaaggccg aggccgcagt ccgaagtggc tggctgagca
      961 ggttgcccgc taccgccgcc tctgtgacgg gacggaaacc ctggttctgg gaaaagaaga
     1021 ccagaccgtc acccgaggtc acttcttcca tggaatttta taatacagga gtattcatag
     1081 atgaataaac gaagtatgag aattttggga ttctaccaga ttcagcacat gttggtgcgc
     1141 ctggctccat cccgcttgtc gaaggaaatc gcccgtcgtt tgaaaccgat gagtgatgcc
     1201 gaagccattg accggcagtt gaccgacacg gaagaagccg tccgctgcac gcagaccgag
     1261 accggcacgc cttttggcgg catcgtcgac atccggcagg ctctggaaaa agccaagcgc
     1321 gaagtaaccc tcgattctcg tgaatgtata gatgtatgga acaatcttca gcattacagt
     1381 gaaatcagtt ctttttttgc tgaccgggca gtagaatacc cgcacctggc tgaacaggcc
     1441 gaagtcatcg gtgacttttc gcagctttcc cattcttttg ccacggtctt tgacaacaac
     1501 caccagattc gcgacaatgc gtcacctcag ttgatgcgcc ttcgcagccg catcgtcgaa
     1561 ctggaacggc agacgaagcg ctacgtcaat aacgtcctgc aaaataaaga gtaccagaaa
     1621 tttttccaag atgccttagt caccgttcga aacaaccgct acgtcattcc catcaagcag
     1681 gaataccgcc atgcctttcc cggcatcgtc cacgatacgt cggccagtgg ttcaactctc
     1741 tacatcgaac ccttggccat cgtcaacgcc aacaacgacc tgcagtcggc ccgcattgaa
     1801 gaaacgcggg aaatcgaacg gattttccgc cgtctgactg ctaaggtcca acagcagtac
     1861 acagaactct atgacagcac cctttgcgtc ggtcagttgg aatttatttt tgccaaggcc
     1921 caattagctc ggcagatgaa ggcctgccgt cctgccgttt ccaagacgcg gcagatcaag
     1981 ctcatccagg cccgccatcc cctcatcgat gccaagtatg tcgtgcctaa tacgatcgtc
     2041 ctcggcggcg actaccggat tttgctcatt accggctcca ataccggcgg taagaccgta
     2101 tccatgaaga ccttgggcct cttggtcctc atgcaccagg caggactttt tatccctgtt
     2161 gaggaatcgt cggaactgcc cgtcttccgc gacgtctttt ccgatatcgg tgacgaacag
     2221 gatatctctc agaacctgag taccttttcc agccatatga agcagctggt ctatatcttg
     2281 aagcactgcg gtccggacga cctcatcctg gccgacgaac tgggctccgg gaccgacccg
     2341 gcagaaggca gtgccattgc catcgccatc ttggactccc tctatcagaa ggaatcctat
     2401 gtcatggtca cgacccatta caatgatttg aaaaactacg cctataatac gccgggtatt
     2461 gaaaacggcc acgtcgaatt cgatatggaa accctgaagc cgacgtataa actgcgcatc
     2521 ggctcggccg gcagctctca tgccttcagc atcagcgaac ggctcggcat gcctaaatcg
     2581 gttctcgaaa aggccaagga actgcgcagc aaagcccagg atatggatat ggaaaaagtc
     2641 ctgacccagc tcaatgccca ggctaagaag ttggacgaag aacaggcaga actgacgtat
     2701 cgcctgaagg aagctcggaa attagaagac gacctgcgtc gggaaaagga caaggtcacg
     2761 tccaagcggc aggatatcat cgatgccagc cgccgtgatg ccgtcgacct taaacgcagt
     2821 ctccgcatcg aagccgaaaa gattatccgt gaactcaagg cgcagaccaa agagaccttg
     2881 tccgatcgcg aaaaggcgag ggccattgag caggctcgtc gggcaatcca gcagatttcc
     2941 ctgcccgaag cggaaaaacc gcaccgcgat cccatcgacc gcagtcagtt aaaagtgggg
     3001 cagcaggtct tcgtcaacac cttggacagc atcggtaccg tcgaagacat gggcagcaag
     3061 caacttaccg tatccctgcg gggcatgacg gtccgcgtca aattcagtga cgtcagcgct
     3121 ccttatctcg acgaattgaa gaaagaaaaa caggctgaaa agaaagctgc tgccagctct
     3181 tcctatcggc ccatccgctc gtcccgggtg gcgacggaac tcaacatcat cgg
//
LOCUS       DAWDXZ010000157         3228 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6558_length_3228_cov_7.555941, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000157 DAWDXZ010000000
VERSION     DAWDXZ010000157.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6558_length_3228_cov_7.555941"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          765 a          952 c          705 g          806 t
ORIGIN      
        1 gccatcgctg ccaaaggcat gatagccgat gacaaccata tttcttgccc gaatatcatt
       61 gtcatccgag ttcttttcat cgggagataa agagccgata ataatagaat ctgtaagctc
      121 cgtattgttt cgattatccc caataacaat cccggcagcc gtacctctga tattattatt
      181 attaccgata accgttaaag cgtcccattc attgcgatac gtatcgttac cggtaatcac
      241 attgccgcgg ccaatggtta agtcggaaat attcggttca cggccttccg gatgaccata
      301 gtcagtaacc gtaccgacat ctctagcaat tatcttgtta ttatcgccga tgacgttttg
      361 cttccgactg ccccgcactt cgttgttatt gcccaaaata cgcgtatcaa ggctcgcttc
      421 atatcttgtt ccgccatcgt aaggatagct ctttgtcacc ttattatcat tgccgacaat
      481 attgacacca ttgctgtttt caactgtatt cgtgtcaccc atgacatttg catcttcact
      541 gccggttaca gtattttccg tccccacaac gcgagctgtt ttatcatcgg tcacagtatt
      601 ggtcgtaccc gtcgccgttc cgctgtcgac gacaatatta tctgctgcat aggccgacag
      661 ccccattccg gacatgctca gccctgccaa aaccatggct gccaaggccg tctttttcac
      721 gacacgactg ccattactct tggtccggct tttcgccatt tccgacacga cgacatagct
      781 gttttttgct cgactccaaa taacccgata aatcctgttc atcaaagaat ctcctttcta
      841 atccattacc cattcgtttt caccttacaa tataagtata catttatttt atataatcgt
      901 aaagttagtc attttatggc caatattatg tatttttata gtcaatcaat aaattctccc
      961 gacaacagtt aaaacagctc atgcattttc acccatacag tcgatgatgg gccgctaggt
     1021 atggtacaat agccctagac tacatggaaa ggaagggatg ctcaatgaag ttcagctcca
     1081 cttatatacg gcctgtaaaa tgggcatttt taagcgcctg tctgctctcg ttgattcacg
     1141 tcatcagtgc cctggccgtg ccgactttaa cggccagcat catcaacgaa ggcgtcctgc
     1201 aaggcgatat cgacgccatc agccgcctca tcatcgccat gatggccgcg tcgttggtaa
     1261 ccctcatggg ctccctgggc gccgtatact ttagttctca tatcgcctcc cgcgtcagcc
     1321 tccgcatgcg ccaacaattg gtacaggctt tacaggactt ttccctgtcc gatttttcta
     1381 aatacggcac gggtaccctg ctcatgcggg ccacgcgaga cgtcgaaaaa atccagtccg
     1441 tcctcgccga aggcatcaac atgattcttc ccatgcccct catgattctc gtcggcctgg
     1501 gccttacctt ctataaaagt tttgaaatgg ggctcatcat cttgagcctg atgatcctca
     1561 tggtcggcct catgctcgtc atccaaaaaa gggcccttcc catcgtcatg gccgtccagc
     1621 aacagttgga caagctcacc gatctggtcc gcgaccatat catcggcatg tcggtcatcc
     1681 gggcttttaa ccgcagccgc gacgaaaagg ccgctgaaat ccggatgttt tccgatttat
     1741 cggccaaaga aacgaagctg gcccggacct acgccatcgg cctgcccagt atcctcatca
     1801 tcttcaatat gagtaccgtc gtcatcttgt ggctcggcgg ctaccaaatc gattcgggac
     1861 aactgcagat tggcgatatc atggccatca tcgagtacgc caccctcatt ttgatgaacc
     1921 tcatcatggc cgttttcgtc ctcctcgacg tccccgaagc cgtcatctgc tacggccgga
     1981 ttcaggaatt attaatacac gccgaaaaga ggctggcctc ccaagaaacg atgagtctcc
     2041 cctctgacaa cgagccgtta aagaaggacg agcacgaaaa aagtaccgca gaggcttccg
     2101 gcaaagagcc gccgctttta gaattccgcc acgtcacctt tcgctatgac gacgccgaag
     2161 aagcagcttt aaaggacatc tcctttaccc tcgaagccgg tgaaaccttg gccgtcatgg
     2221 gcgatatcgg ctccggcaaa agtacgctgg ccaagcttat tttaggctta taccccattg
     2281 aatcgggaaa catcctcttt gacgggaagt ctattttcaa gcagccccta gaatccattc
     2341 gctcccaaat cggctatgtg ccgcaaaaag cctatctctt cacgggctcc attgctatga
     2401 acttagccta cggcaaggcc caaaattcag acgagcccct atcggcggat cagctaacag
     2461 aagcagccgt cctcgccggc gcccatgagt tcatcagccg ttttgaagac ggttacgatc
     2521 atctcctctc ccaaggcggc accaacctct ccggcggcca gcggcaaagg cttgccatgg
     2581 cccgggcctt agtgagaaag ccgcggctgc tcatctttga cgacagtttt tctgccttag
     2641 acggcattac ggagcagcaa gtccgaaaag ccgtagccga agaaatcaga gacggcgccg
     2701 acatcaaagg gctgtcagaa aataaggacc acacggggcg agccttcatc tccatcgagc
     2761 agaaggtcag cgccgcccga aaagccgata aaattctggt catcaatcag ggccaggccg
     2821 tcggtgtcgg cacgcacgat gaattattgg cccgctgccc catttatcag caaatcgtga
     2881 cgtcacagga ggtggcctta tgatactcgt caaacgcttt ctccgtgact cccgtagtca
     2941 gtggtggcga ttggccgtcc tgaccttggc cctcatttta gcggccagtt ttgaaatcat
     3001 ctccccgcgc ctcttgggca acatcgtcga cgccatcgtt cacggcatcg aggagcagca
     3061 agaaatcacg gccatcttag acggcacgac caacaccgtt ctgctgctgg tcttctttta
     3121 cagctgccac gccgtattta cctacgccag cgaattcatc atcgccgggg catcgcagca
     3181 catggtcctg gccctgcgca gccgcgtgag ccaccagctc catcacct
//
LOCUS       DAWDXZ010000158         3223 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6566_length_3223_cov_7.525253, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000158 DAWDXZ010000000
VERSION     DAWDXZ010000158.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6566_length_3223_cov_7.525253"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          780 a          901 c          822 g          720 t
ORIGIN      
        1 ttacctggcc gatggaacgc cgcaaagctt tcatggacat catcaaccgc tatgacttcc
       61 cggttctcga agacgatccg tacggcgaac tccgtttcga cggcgacaaa gttccgtccc
      121 tcaaatcgat ggataccaag ggcaacatca tcttcctcgg ctccttctcg aagatcctca
      181 tgccgggcct gcgtattgcc tggatggttg ctgatccggt catcattgat aaagtcgtta
      241 agctgaaaca ggccgtcgac ctccagtcct cgtccttcgg tcagcgccag acttccttct
      301 tcatcgatat gtacgatctc gatgatcacg ttgcaaagat caaagaattg tacaaaaaac
      361 gccgcgatct catgtgcgat tcgatgaaag aatacttccc tgaaggcatc acctttacat
      421 accctgaagg cggcctgttc acgtgggtaa cgttgcccga aggcatggat gcgaaagaac
      481 tcatgcctaa agtcctagcg aaaaacgttg cctacgtgcc gggcggtccg ttctacccgc
      541 atggcggcaa tgctaaccac ttccgcctca actactccaa catgccggaa gaacgcatcg
      601 tagaaggtat caaacgctta gctgaagtac tgaaagaaga attggcaaaa taagcctaaa
      661 aatgatatga tataagggag ctgtcgcttt gcggcagctc cctttgtacg ttcactcaag
      721 gaaggatgcc ttgtttcatg cttcaaattg aatcattgtt aaaacagtat tttggctatt
      781 cgtcattccg gccgggccag cacgaagtca ttcagaccct gctcgacggc cgcgattgcc
      841 tggcaatcat gccgaccggg gcggggaagt cgatctgctt ccagcttccg gccctgatga
      901 tgcccggcgt gaccttagtc atttcaccgc tcatttccct gatgaaagac caggtagaca
      961 gtctggtcaa tcaggaaata ccggcgacat atatcaacag ccagtgtacc tttgaagaag
     1021 tgaaggcccg ctttgctgcc atccgtgccg gccgcatcaa gctggtctat atctcgccgg
     1081 aaagactgga gaatcagttc tttacggctt tcatgcagac cctgcccatt tccatgttca
     1141 tcatcgacga agcccactgc gtctctcaat ggggccatga ttttaggccc agttactgca
     1201 ccgtccgcga ctggatcgca gcccttccca agcggcctgt cgtcggggct tttacggcga
     1261 cggcgacgga aaaggtcaag cacgacatga tgtccctctt aggccttcaa aaggaacgca
     1321 tttttatcgg cggcttcgac cggcccaacc tctatttccg cgtcgttcat gccaaggcta
     1381 agatggagtt caccttgtcc tacattgcca agcataaaga ggacagcggg atcatctatg
     1441 cggcgacgcg caaggaagtc gaccgcatct gtgatgaact gaatcggcgg ggcatcaagg
     1501 ccggccgtta ccatgccggt ctcagcgacg agatgcgccg ttccatgcag gaagccttta
     1561 cctacgataa aatccaggtc atcgtagcga ccaacgcctt cggcatgggc atcgacaaga
     1621 gcaatgtccg ctacgtcatc cattaccaga tgccgaaaaa catcgaaagt tactaccagg
     1681 aagccggcag agccggccgc gacggggcac cgggggactg tatcctcctc tttaaccgcc
     1741 aggatatcat gatccagaaa ttcctcatcg agcaatcgac tcgcgacgcc aaacagcagg
     1801 aggtcgaatt ccgcctgctc catgccatgg ttcgctactg tgaaggcaac cactgcctgc
     1861 gccattatat tctgtcgtat tttggtgagc atccgtcgtg ggagcactgc gataaatgcg
     1921 gcaattgtga ccaggaaacg gtcgaagagg acatgaccga acaggtgcgc agcatctgcc
     1981 tctgcgtcga cgaactgaaa gggcgctttg gcctgaccat ggtgtccgac atcctgaaag
     2041 ggagtcagaa tgctaagatt cgccgctatg gctttgaccg caatgccgtc ttcggcatgc
     2101 tcggtaattt ttccctgtcc gaagttcgcg atatggtgcg ccagtgcatc gacgacggct
     2161 atctcgagca gtctgacgga aagtatcccg tcgtctccct gacggcagac ggaaggcagg
     2221 cgatgggggg ccataaacgc atcgttcagc agaaacgagt cgttgaagag attccggcgg
     2281 ccattcccaa acgccggcaa aaacgcagtg ccgggacctt tgacgaagag accctgcgcc
     2341 ccttgttcga caccctgcgg gccgttcgcc gcgacttagc caaggacgaa aacattccgc
     2401 ccttcgtcat tttttccgat gccaccttgt gggatatggc agcccttaag ccgagcagcc
     2461 tcgacgccat gggtgacatc aagggcgtcg gcagttttaa attgcataaa tatggccgca
     2521 gtttcatcag cgccattcag tcttatatga ataatcagta attttgaggt gatactacat
     2581 gagcatggcc gatacacaat acctgaatat cgtcgaaaat atcctcgacc acggctattt
     2641 cgacaacaac cgcacggggg tgcggacgta caagctgccc catcagatga tgcagttcga
     2701 tttggaacat gaatttccca tcttgacgac gaagttcgtg gcctttaaga cagccgtcaa
     2761 agaaatcctt tggatttggc agctccagag caacgatgtg cgggagctgc agaagatgaa
     2821 ctgccacgtt tgggacgaat ggatgcgtcc tgacgggacc atcggcaagg cttacggcta
     2881 tcagatccgg aagtataagc aggtggataa gctcttggat accctgaaaa acgatcccca
     2941 gagccggcgc atgatcgtat ccttgtggaa tatcgaggat ttggatgaca tggccctcca
     3001 gccctgtgcc taccagacct tgtgggatgt cactgacggc cgcttgaact gtatgctcat
     3061 tcagcgcagc ggcgatatgg gcctgggcgt acccttcaac atgacccagt atgccgttct
     3121 catccacctc attgccaggt ctgccggcct ccgtccgggc ctctttacgc acgtcatcaa
     3181 caatgcccat atctatgaaa atcacgtcga agccatgaag acc
//
LOCUS       DAWDXZ010000159         3212 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6583_length_3212_cov_9.294583, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000159 DAWDXZ010000000
VERSION     DAWDXZ010000159.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6583_length_3212_cov_9.294583"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1364..1373
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          732 a          888 c          828 g          754 t
ORIGIN      
        1 gcatatccat cgtgatcccg atgttctcga tacttggttc agctcggccc tttggccctt
       61 ttcgaccatg ggttggcctg ataagacggc tgatctcgac cagttcttcc cgaccagcgt
      121 cctggttacg ggctacgaca tcatcttctt ctgggttgcc cgcatgatgt tcatgacctg
      181 cgaattcatg aaggatattc cttttgaaaa cgtcttcatc cacggcctcg tccgagacag
      241 ccagggacgc aagatgagta agtctctggg caacggcatc gatcccctcg gcgtctgcga
      301 agaatacggc gccgatgccc ttcgctttac cttggttacg ggcaatacgc cgggcaacga
      361 catgcgtttt tacatggaac gagtcgaagc taaccgtaac tttgccaata agatttggaa
      421 tgcctcgaag ttcgtcatca tgaaccttac cgactttgac gaagccttcg ttcccgaaga
      481 aagcgatttg accttggccg accgctggat tttgacgtcc tttaatgaaa cggcagccag
      541 agtcacggaa gacctcgata aatttgaact gggcgacgcc gccgatgccg tctataactt
      601 catttggagc tcctactgcg actggtacat cgaacttgcc aagaagcgcc tctaccaggc
      661 cgccagcgac cgcgacaagc acaccgtcca gtacgttttg gtctacgttt tgacccatac
      721 cttggaaatg ctccatccct tcatgccctt cgttacggaa cacctttggc agcatctgcc
      781 tcacgaaggg gaaagcatca tcgtcgcccc ctggccgaag gctcaggata agtggaattt
      841 tgccgatgac gcggctacta tgaacaccct catggaagct atcaagggaa tccgtaacct
      901 ccgagccgaa tcgaacgttc ccatgggcaa gaaggctccg gtcatcttag caccggcaac
      961 ggaagacttg gccaagacct tgaacgatta tgaagattac ttccataccc tggcttttgc
     1021 cgacaaagtt accctccttt cggctggtga cgccaaacct gaaaacgccg tcgtcgccgt
     1081 cgtgcccggc atcgaagttt atttacagct caaagacctg atcgacgtcg aaaaagaaac
     1141 ggcccgcgtc cagaaagaac aggaaaagct ccagaaggaa atcgaccgcc tcgacaagaa
     1201 gctggctaac cagggcttcc tggccaaagc tccggcagcc gtcgtcgaaa aggaaaaggg
     1261 gaaactggcc gattatcagg aaaaaatggc tgcccttacg aaacgcatgg aagatttggc
     1321 aaaactctaa gaggctctca tgacatacga agaagcaata cacnnnnnnn nnntatttag
     1381 aagctgccaa cgtctttggc atccagttgg gactcgggcg catccaaaat ctcttggaac
     1441 gcctgggaaa tccccaaaac aaatataaga ccatccacgt caccggtacc aacggcaagg
     1501 gatcggtgac ggccatgatc gcatcggccc ttacagaagc gggttttaag accggccgct
     1561 acacgtcgcc tcacttggag tcctatacgg aacggatcca catcgaccat cacgacattt
     1621 ctgaggatga ctttgccgct gccgtcgccg tcgtcaaaga cgccgtgacg gccatggtag
     1681 ctgacggcgg ggaagggccg acagaattcg aactgattac ggcggccgcc ttctggtatt
     1741 ttgccagcat cggtgtcgac tatgccgtca tcgaagtcgg actgggggga ctcctcgatt
     1801 cgacgaacgt catcgttccg gccgtatcgg tcatcaccaa cgtggctatg gaccacatga
     1861 aatactgcgg caatacgatt gaagccattg ccgagcagaa ggcgggcatc attaaagaag
     1921 gggtacccgt cgtcacgacg gcggaagagc cggcactatc ggttatcgcc cgcaaggcct
     1981 atgccatgca cagccggctt tatgcccttg atcacagctt cgacgtcgtc ggcaatgccg
     2041 gtcccgaaga cccggcgaca ggccagatgg tgaccgtccg ggaaaaattc ggctttgcca
     2101 ttacgacggt cctgccttta ttggggaaac accagcggat caactgtgcc gccgccgtca
     2161 tgaccttgct cctcttggcc cgccatgaga aaaagctgac tcggacggcc cttgaaaatg
     2221 gtatccgcca cgtcgagtgg cccggacgat ttcaagtctt taaggccggc catacagacg
     2281 tcgtcctcga tggcgctcat aatccggccg gcattacgac tttttgcaag acctatgaag
     2341 acgtctttgg agaccgcaag cgggtcttcc tgttttcggt cctagccgat aaggattaca
     2401 gccaaatggt acgggaactc tttcacgaag atgactacgt cgtctgtgca ccggcaccga
     2461 cgacgcgcac gtccgatccg aaggccatgg cagaacttgt gccttgtaaa gccgattggg
     2521 ccgaaagtct tgctgaagga ctcgataagg ccttcgccgc tgtaacaccg gatcaggtcc
     2581 tgtgcattgt gggatcgctc tacattcaag gtgaagtacg ggcatatctt cggaaaaact
     2641 atcagctttt ttaaaccgtc ttgtcggctt ggaaagacgc gttacctatc gttttacaga
     2701 cctgtcagca ggctgtcgaa aaaggagtgt ctccctgtgg agatactcct ttttcgttgc
     2761 gctggagtgg accttatgat aaaataaaac atatatttac ggaggagcga acctatatga
     2821 agtggatgat acggaccggc agtacgcctg ctgagcgggc acggctgcgc gtcgtctatg
     2881 ccctggccgg gactgttttt tttatgcccc tgaatctttt tgtgatggag ggctttttcg
     2941 tcgtggcctt gcttctcggt ctttattata tgatgcggta cccgcgcctg cctcttcgaa
     3001 aagggcctct ttccatgcct gccgtcgggt ttgccgtcgc cgcttttttg tcgctcatcg
     3061 gctcgcccca tttttttatg ggaatggcat tctacgtttt tacgattctg cagtatatcc
     3121 tactttatta cggcatcttg gtctttgtgc accgttcgtg ggaacggagg atgctctttt
     3181 cggccctctt gttcagtgcc tttgccgtcg cc
//
LOCUS       DAWDXZ010000160         3202 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6599_length_3202_cov_6.865904, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000160 DAWDXZ010000000
VERSION     DAWDXZ010000160.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6599_length_3202_cov_6.865904"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1070..1079
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          866 a          859 c          809 g          658 t
ORIGIN      
        1 atatagtctt agaggtcaaa tagtcagaac gctaagaaaa gtcaaatgag cagaggtgat
       61 atcgtcatga acttagatgc acgcaaacag aagatactgc aagtcatcgt tcaggactat
      121 atcgcatctg ccgaacctgt cgggtcgcgg acgattgccc gtaagtatga tttgggcatc
      181 agcccggcga cgattcgcaa cgaaatgttc gatctggaaa tgatgggata tttggaacag
      241 ccccacacgt cggccggccg tgtcccgtcc ttgaaaggct atcggctcta tgtcgactgc
      301 ctcctcaagc cgacgaagat tacggaagcg gaaaagcaat tcgtccagtc ctggtttcag
      361 gataagctga gcagtgtcga tgaaatcttt cagtcgacgg ccaagctctt ggcccggatt
      421 acacacaacg tcaccttgat tatggcgtcg gagcggctca actcgaagtt gaagtacatc
      481 cgctttttgc cccttgatga caggcgggcc atcatgatcg tcgtcaccga cacgggacag
      541 gttgaaaatt gtatttaccc gaaacccagt ggggccagca tggacgattt gaacatcatc
      601 gcccagaagc tgaccaacta tttgaccggt acggccatgg accgcatcga tgaaaaggct
      661 atcgaaacat ttcatgaaac catcgtcgac gacgtcgaac tctatcgact ggctttccgg
      721 gccatggagc agacccgccg ccaaggcgag catttctaca aaggcggtac gacggaactg
      781 ctcaataaac cggaatttaa ggacgtcgac aaggccaaga gcctctttac catgctggaa
      841 gaacaggaca tcgtcgccaa catccttcac gatgaaggcg aaggacagtc cgttaccgtc
      901 cgcatcggcg aggaaacgaa gctgtcgccg attaacgact gcagcatcat tgaagcgacc
      961 ttcaccgatc acgatgtcgt tctcggcaag ctggccgtct tagggccggc acgaatggaa
     1021 tatgctaaat catcggcctg ttggatttca tgaaacagca tatgacacan nnnnnnnnng
     1081 cgtactgctt ccgaaaagct tcagatttcg gaagtcgtca aaatggacgt cctgggccgg
     1141 atcctgccgg tcatcgacaa ttttgaacgg gccctcaagg ccccgacgga tgccgcttcc
     1201 gacgacgtac actcctttat cgaaggctat gaaatgattt ataaacagct tatggccgtc
     1261 cttgaaaagg aaggggtcac gaaaatcgat gccgtcggca aacctttcga ccccaattat
     1321 caccaggccg tcatgcgcgt gcccagcgac gaatacgatg acgacatcgt cgtagaagtc
     1381 cttcaggaag gctatctcct gggcgaaaag accttgcgtc cggccatggt taaagttgca
     1441 tttaacggtt aattatcgtt tagaataaga ttggaggaat cttttatgag caaagttatt
     1501 ggtattgact taggtacgac aaactccgta gtcgctgtta tggaaggcgg cgaacctacg
     1561 gtcatcacga atacggaagg ttctcgtctc accccgtcgg ttgtcggttt ctcgaagacc
     1621 ggcgaacgcc tcgtcggcca gttggcaaaa cgccaggccg tttctaaccc ggaaaacacg
     1681 attgcctcga ttaaacgtca catgggtgaa aactacaccg ttgacgttca gggcaaaaat
     1741 tatacgccgc aggaaatttc ggctatggtc cttcagaaat tgaaggaaga cgctgaaagc
     1801 tacttgggcg aaaaagtaac tcaggccgtt atcaccgttc cggcttactt taacgacagc
     1861 cagcgtcagg ctacgaaaga cgccggcaag attgccggtc tcgacgttct ccgtatcgtc
     1921 aacgaaccga cggcagcagc tctcgcttac ggcctcgata aaggcggcga cggcaagatc
     1981 ctcgtcttcg accttggcgg cggcaccttc gacgtctcga tcctcgaact gggcgacggc
     2041 gtctttgaag taaaggctac caacggcaac acgaaactcg gcggcgacga cttcgacaac
     2101 aagatcatgg actggatggt tgacgaattc aagaaacaga ccggcatcga cctctcgcaa
     2161 gataagatgg ctgaccagcg cttgaaagaa gctgctgaaa aagcaaaaat cgaattgtcg
     2221 acggtccttt cgaccaacat caacctgcct ttcatcacgg ctgacgcttc gggcccgaaa
     2281 catctcgacc tgacgttgac ccgtgctaaa tttaacgaac tgacggaaga cctcgttcat
     2341 gctacgatgg aaccgaccaa gaaagctatt gccgactccg gtctcagcat cgacgaaatc
     2401 gacaagatca tcctcgtcgg cggctccagc cgtattccgg ccgttcagga cgccatcaaa
     2461 tcgatcctcg gcaaagaacc gagcaagggc gtaaaccctg acgaatgcgt tgctatcggc
     2521 gctgctatcc aggccggtgt cctcgtaggc gacgtcaaag acgttctcct cctcgacgtt
     2581 acgccgttgt ccctcggtat cgaaaccctc ggtggtgtct tcactaagat catcgaccgc
     2641 aacacgacaa tcccgacttc cggcagccag gtattctcga cggcagccga taaccagccg
     2701 tccgttgatg tccacgtcct tcagggcgaa cgcgaaatgg cagctgacaa caagaccctc
     2761 ggccgctttg aactgacggg cattccggca gctccccgtg gagtacctcg tatcgaagtt
     2821 accttcaaca tcgatgccaa cggtatcgta aacgtttcgg caaaagacct cggaacgggt
     2881 aaggaacaga agatcaccat caaatcctct tcgggcctcg acaagagcga aatcgaccgc
     2941 atggtcaaag aagctgcggc tcacgaagct gaagataaga agcgtaaaga aggcatcgaa
     3001 gctaagaaca acgccgactc cctcatttat caggctgaaa agaccatcaa ggatatgggc
     3061 gacaaagccg acgctgctaa agtttcggaa atcaaagata aaatcgctga cttgaaagaa
     3121 gctgtaaaga gcgacgacgt cgataagatc aaagccgctt cggaagcttt gaccaaaccg
     3181 ctttacgact tgaccagcga aa
//
LOCUS       DAWDXZ010000161         3197 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6606_length_3197_cov_6.971674, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000161 DAWDXZ010000000
VERSION     DAWDXZ010000161.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6606_length_3197_cov_6.971674"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1078..1087
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          947 a          592 c          697 g          951 t
ORIGIN      
        1 atactgaagg aatagaaagt tgtgggagat ttccaaaaaa attatggaat tatgagttac
       61 ttgagtgata tgttcctgaa aaatgggatt tcatactgtt gaattcgttg aaaacaacga
      121 caggagtact aaaggcactc ctgtcgaaga agtaatgatg gaaatggatg aaagaagtag
      181 acaaacgctg atttctcgtg taatcagttg agataactat acttactcaa ctagttgtac
      241 gggattttta tcattttttt acattccgaa aaggcattgg cggaaacggt cggttttttt
      301 ttgccttaaa gaatggtata atgaattatt gtaaagtccg tgacggagaa gaacctccgt
      361 tttttcgtgc gtatataata caataataat gacagagctc atgctttgcc gtgaggagga
      421 actgttttga agtgttcgcc gcagtcatgt tcaatttcga ttaaaaataa gtacagtaat
      481 gaggtgttgc ccgttgtgct ggccgttgcc gattacattg ctatttggct ggcggaatac
      541 gtgtcgtatg gcctgcggga ctgcctgatt cccaatacgc attttcatct gtcgtggctc
      601 agcattcacg tcatcgtacc ggcggtatac ctgctgtttt tgaaactgca tcacctctat
      661 acacggcgca tgcagttttg gcagatcatt tctaatctct ttaaggcgac cttctattcc
      721 attgccttat taattttctt catctacatt gcccaaacgg cggcgtcgac gtcgcgactg
      781 ttcgtgggct tattggccat cacttctttt gttttcttgg tcattgaacg gtacgtcgtc
      841 aaacgcatct tgatccgtat gaagctgctc cagctcccgg tcctcatcat gggtgccgga
      901 aagacggcag ctctcatgtt ggaatacttc aagcgtgata ccggcgtcgg atacgaattc
      961 atcggctacc tcgaagacca tacgccggat ccgagagtgg aggccatgat tccacactta
     1021 ggaaccttcc gcgatgccga gcaggtcatt caggtcaccg gtgtccagca tgtcatgnnn
     1081 nnnnnnnccg cgtccaaccg ctggtgaaaa aagtggcctt tatcccggac atgggcagcc
     1141 tgcctttggc aactctcgaa acgaaccccc tcatcgacgg ccgcatcatc tccatcagtt
     1201 tccggaataa cttatcccag tggtacaatc gggctatcaa gcggattttt gatttagttt
     1261 gcaccattat tggcatcatc tgcctgtcgc cgtttttctt agctgtcgcc ttttggatcc
     1321 gtctcgattc gccggggccg atcatcttca aacatcgccg tatcggccgg aacggtaaag
     1381 aatttaactg ctataaattc cgcagcatgt gcgtcgatgc tgacgttaaa ttgaaagaac
     1441 tcctcgaacg agatcccgaa gcccggaaag aatgggagac agaattcaaa ctgaaaaacg
     1501 acccgcgtgt tacgagaagc ggagcctttt tgcgcaagac cagccttgac gaactgccgc
     1561 agctgttcaa cgtcctgaag ggggacatga gcctcgtcgg accgcgcccc atcatacggg
     1621 acgaaatcaa gaaatacggc gcctttatta aagactttta tatggtccgc ccgggcgtca
     1681 ccggcatgtg gcagaccagc ggccgcagcg atacgaccta tgacgaacga gtccagatgg
     1741 atacctggta cgtgagaaac tggaacgtct ggtttgacat cgtccttatt tggagaactg
     1801 ttgctgttgt gttaaagcat aagggcgcgt attaaaacga agattattta tcgaaatatg
     1861 gggaaatatc taaaatttct acccagacta cttttgtata atcgctatgg aatgatagac
     1921 ttctataact caattagtgc atgtataatt atggcttttt gtataatgga ggacaattgt
     1981 gaaaataggt tttttgtttg gtgcaggtgc agaagttcct tataatatgc ctacaggggg
     2041 gacttttgcc ctcgatattt ttaggcagga tgtcagtagt gcaaaaagtg aatttaaaga
     2101 aatgagaaat cacatagatt ccactactgc atatgcaaca gaatggctcc ctcaaaaata
     2161 tgatgataat aatatttttg tatttggtaa atctgttttt gaaaatatta ttaaagatac
     2221 agttgaacat aatagagaga aaatcatcaa tagtttaaag catttagatg aattatctca
     2281 acgagtcatt cgacatcttg aactgaaatt accaaaggcg aacattgacc agtcttttga
     2341 aaatatcctt ggaagacctg tagataatgt gagtgttgca caagatataa aattaaaaga
     2401 tcaattcggg ggagggaatg aattattttc aagtcattat ttttcgggac tgttattaat
     2461 gtataaagat aaaattgcgc cagagtataa aaatgaccta gggaaaatta ttgtatctct
     2521 tttacaatta cttttaggtg cattgggtga aaatttagct agacagatta atgatagtcc
     2581 tttttcaaag aaaaatgatg atattgattt gtttgatgat atgggggaat tatttcagct
     2641 aaattattca tttgcagggg ttgatggact tagatattta tttgaaagaa gagagatttt
     2701 atcttcagat tattcagatt ctgagattgt tattatgttt gccgaagagc tactagaggg
     2761 agtttttgca gatgtattgg attataaaac attaattgac tctaattggc actatctgta
     2821 ttgtcctaaa tcggaatgga gtaaattttg taaaataagc atatttctgt tgactgtaag
     2881 aaattatata aaacagaaat gccaaaatat agatgaggat gcaaagggat attacgatga
     2941 cttaagaaat tgctcagaaa tggatgttca agtgactgct attgctacta ctaactatac
     3001 aaatctaatt aaagaaaaga ccccagaaga ttttcaagat aaaatttatt ttttaaatgg
     3061 gtatacagaa caatggtatg atccctatat aaataagatt ggaactcaac aagaattaga
     3121 ttctcgagaa aaacattttc gtgttcccct cttatttacc caaagtggaa caaaaccgat
     3181 gacatcaatc tttatgt
//
LOCUS       DAWDXZ010000162         3196 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6611_length_3196_cov_5.041070, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000162 DAWDXZ010000000
VERSION     DAWDXZ010000162.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6611_length_3196_cov_5.041070"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1218..1344
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1615..1624
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          865 a          603 c          729 g          862 t
ORIGIN      
        1 agtcataaat ttccgactaa aaataatatg actaaaagaa atctgagtaa aggagaaaat
       61 atgtttattg gcagacagga tgaattagag aaattggcgg aaatgtacca ttcagatcaa
      121 caagaagtcg tccttattta tggcagacgc cgcgtaggga aaagtgagtt gataaaacaa
      181 tttttgcaag gctgtcagac aaaaggactg tactatcagt gtaagcgaac gacggagatg
      241 aataataccg agagcttatc ggctttgttt tccgaaatat atggctatcc gccgctgtct
      301 tttgacagtt ttgaagcttt gttggatttt ttctttcaac aggcttgtcg ggaaaagagt
      361 gtacttgttt tggatgacga ttcatttgca tcccatggac tattttgaaa cagcgcagtt
      421 ctatccttca ttttctcccg ttgataagat tcgcttgtac agtgtttttg gcggcattcc
      481 ttactataac cggcttgtcg atgagactac gagcgttcgt gacaatattc ttcgccttct
      541 cgttgtaccg ggtgcgcggt tagaaaatga ggttcaaatg tatttattgg gagaattggg
      601 gaaatttgtc aatgctaatg aagcctttga agccttagcg cgaggttttc ggcggtataa
      661 ggatattttg tcccaatctc atatttccag cagcccggta ctggctgagg tattgaaacg
      721 actgattttg atggaactgg tggaaaagac ggctcctatt aatgatgctc aaaatgcgaa
      781 aaaaaccagc tatcatattt cagataatat ggctggcttc tattaccgat atcttttccg
      841 atatagttca cagcggcgta ttatgtcgcc tgatgccttt tatgatcgat atattgacac
      901 tgattttgaa gaacagcttg ttccgcattg ttttgaagat atttgtcgtc aattccttat
      961 ccgaaaaaat agaaggggag agctgcccga aatattcgat gccattggcc gctactatta
     1021 tgatttaccg aaagaacatc gtaatggcga attcgatgtc gtaacccatg accggaaagg
     1081 atacatcttt tatgaagcga aatttcgtca gtctccattg acggacgcca tgatagaaga
     1141 ggagatacga caggtacagc agacggggtt agcctgctat caatacggat ttttttcccg
     1201 ttccggctat caagttannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnttctag tttggggcgg ggatcaactt tttcttgtac
     1381 ctcaggaact tttgcgttac aataaggacg tattggggca ttagctcagc tgggagagcg
     1441 caaggttcgc aatcttgagg tcaggggttc gatcccccta tgctccacca atttgaaaga
     1501 ccatacccac gcagccggga agccgcgcgg gtgttttttt acggtgtccg tgccgtcctt
     1561 ttcgccggct tttaggcgac agaaagtgag tacagacgat gatcgattat catgnnnnnn
     1621 nnnnatcatg aaattttatc ccgtatgaat ccgaaccagc ggatcaacta cgatacggtg
     1681 taccagaaga tggccaagcg ctggcagcag gacggcgtca agccgcgaat tctgctccac
     1741 tcctgctgcg gtccctgcag tacggccgtc ttggatcgcc tggttgaggt ttccgacgtg
     1801 acggtctatt tttttaatcc caacattcat cccgaagcgg aatataagcg gcgggaatgg
     1861 gtgcagaaag agttcattgc cgaatataat gaaaaaacgg gccacgacgt tcacttcttg
     1921 gccgccccat atgagcctaa atcttatttt gaagccgtaa aaggcctgga agacgagccg
     1981 gaaggcggcg cccgctgccg cgtttgtttt gagcagcgca tgaaggccgc tgccgaaaag
     2041 atgaaggaac tcgacttcga ttacttcacg acgaccttga ccgtcagccc tcataaaaat
     2101 tcgcaggtca tcaatgacgt cggccgcgag attgaagcag catacgggat ttcctacttg
     2161 cctacggact ttaagaaggg caacggcttt ctgacctcga ctaagatggc cgtagcctac
     2221 ggactctatc gccagcctta ctgcggctgt atcttcggtg cccgcagtca aggcctcgat
     2281 ttagaagagg tccgcaaaga tgccgaagcc tttttggccg ataaagagaa aaataaatga
     2341 catattgtgt tatcaaagcc ctttcgagag aatgggcttc tttttatggg aagagcttat
     2401 agacacaatt aaaatacgat tagaataaat aataatgata tagttgtgtg gatttacact
     2461 aaaatttgat ttacaatata attacataaa acaaattata ttgattggaa gtggagtcat
     2521 gagtaaagat agacggaata tattgcgtgc ctgtatcttg agcgtattga tgactatgcc
     2581 tgttgccggt gtctgttcgg catcttctta tccttggacc gatgaagata atatcggtgg
     2641 tacatatgaa atcaaaggca gaaaccacgc tatttccttt gatgagagtg aaaaaacagt
     2701 tcaagccaaa gatgatgtaa ttatcaaaaa tggggcagaa gctgatttgg ataacaatga
     2761 tgatacaatg tctatcattt caactgtcgg tgaagatggg aattccttta ccttcgatat
     2821 gaatggaaaa aatattactg ctggcgacta cggttcattt atcaatttga caggtgggag
     2881 taataataag gctgaggttg tgaatgcttg gaatatcacc agcaccacac ataaagctaa
     2941 tctcatatat gctgttggat cagggcataa tatctcctta gatgctagca atgatattca
     3001 gttgaataaa aagaggtctg attcaccggc aactcttatt tctatcgctg gtgataatct
     3061 cttacatcta tcggctggtc gcgatgtgat tctgaccaat gaaaatacta cgaatatact
     3121 taatcttacg gatggaggaa aggcggaggt gcgggccggt cgcaacttat tgtttaatca
     3181 ggctaaaata gaaggt
//
LOCUS       DAWDXZ010000163         3196 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6610_length_3196_cov_8.441897, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000163 DAWDXZ010000000
VERSION     DAWDXZ010000163.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6610_length_3196_cov_8.441897"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    980..989
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          853 a          650 c          795 g          888 t
ORIGIN      
        1 atgccattga tatgttttgg gataatgaga tagacacaac tatgatgctg acaaaagcaa
       61 ggagattgtt tctaatataa tgcgttttta ctaaagtatt caaaattacc acctcttgtt
      121 ttaattatac atatagtata caactaatcg ttacacatat ccattctgag tatcataaat
      181 ggaagtgaat cgcttatttt ttagtgatag aggcataaga taaatcggtt actcttctat
      241 ggcgacaaag ccttatgaca tgttcagtaa atttgaactt gacaagcgat tttgaatcgg
      301 ttaaaattaa aaggtcgacg ggacgtggcg cagcttggta gcgcgcttcc ttggggtgga
      361 agaggtcgcg agttcaaatc tcgccgttcc gaccaagaca tgaccattct ttgaatcatg
      421 taattatgat tcaaagaatg gttctttttt tatagtaaaa ccttgcggaa ctcgattatg
      481 aataattttc cgtaagggga gatatggatt tcttttgaag acgggtacat ggtccgtctt
      541 tttttgtgtg cgtttttaga gaactgggga attttgcgaa gaacaaggtg acttaggcaa
      601 tgtcggtttt tgacaatcca ttcttaaaat agaattagaa aatgctttta tcttatttaa
      661 aactttcttt atatgcgata tattgatata atataaatac gaatagtata gttaccgccg
      721 tcgtattgtt tttttgttgt tcattgttag aggaggtttt tttatcatgg gttatattct
      781 caataaggat cagcaggatc tcgtcgcctt ggcaagggac tttgcggaaa agaaagtagc
      841 tccctttgtg catgaattgg atgaagccaa agaaccgggc caaaaacttt tggatattta
      901 ccaggaagcc gttgatatcg gcttttgcgg cttagagatt cctgaagaat acggcggcat
      961 gggacaggat tattacacgn nnnnnnnnnt tacacggttg ctgctgttta tgaagaactg
     1021 tctaaagtcg acgccggcgt ggctacggct attgcagctt cgggtctcgg tctcaaaccg
     1081 gttctccagc acggtaacga cgctcaaaag aaattgtatg ccgaatattt gaccggtgaa
     1141 aaaggcagcg gtttctgcgc attctgcctg actgaagcca acgcaggttc cgatgcggct
     1201 gccggtacga cggttgctgt caaagaaggc gatgaatacg tcatcaacgg tacgaaatgc
     1261 ttcatcacca atggcggcgt cgcttccgtt tacaccgtat ttgccattac cgataaatcg
     1321 aagggcgtaa aaggcatttc ggccttcatt gtagaacgtg accgtccggg cgtttctgtc
     1381 ggtaaagaag aagacaagat gggtatccgc ctgtcgaata cgacggaagt cattttccag
     1441 gacgtccgca ttccggccga ccacctcatc ggtgaagaag gcaaaggctt cgtctatgct
     1501 atgcagacct tggacctcgc tcgtcccatg gtagcgtccc tggctgtcgg tattgcgcaa
     1561 agagctatta atgaagctgt caaatatgcg aaagaacgcg ttacctttgg caaaccgatc
     1621 atcaaacatg aagttatttc ctttaaactg gctgatatgg acatgaagac ggaagttgca
     1681 cggtcggcta tcatcaactt cctcaacacg tattatgcag aaccgaaacg gagctatact
     1741 cgtgaagcgg ctatcgctaa atgcttcgcc ggtgacattt ctgtagaagt tgctttagaa
     1801 gctgttcaga ttctcggcgg cttcggttac agccgtgaat atccggttga aaaattggtt
     1861 cgtgatgcta agatcatgca gatttatgaa ggcactaatg aagttcagcg catggtcatc
     1921 gccggtttcg tagcggctca aaaataagat gcttttataa gaaaagactg ttgtacttgg
     1981 ccccggctct catttgtgcc ggggccatcg ttaaaaatac cggtgtacga cagtcttttt
     2041 gtgttcgaaa cggtgttttt gaggcgctgt aagacagtgt gtttttaggg gaagcactga
     2101 atttgtgaag gggtgtaaaa tcgatgtaaa gcaaggctga cggcgttgag aaagtttgtt
     2161 tacaggaata taatagttta tatgaataaa aatcatgaaa atggggtgaa ttgaagtgga
     2221 acaagacata tatccatcag cgaaagaact ttatggcgtt ctttgcaggt tacggaagga
     2281 tgtcctgtcg cggggcgatt ctttatatcg gcagtggaaa ccattgattc agcggagaca
     2341 ctttacctat agtgcctgga atttggcctt ttatctggct cttcgctgtc atgatttgcg
     2401 aaagatacag cgctcgctcc ttccctttgg tctgtcgtct ctcggacgcg gcgaagccag
     2461 ggctattacc aatctcgatg ccgtcatggc ttccttgggg cgtatctgtc aggaaccgga
     2521 acagaatctc gttccctatc cgcagcagcg gcgatttttc tatggcgacg gcctcttagc
     2581 gtacaatacg ggccggattt ttgggacggc caagacgggg acgcgcatca tggttaccct
     2641 gcccacagaa gcggcagaag acaacggaat cctcatccgc gacttagtgg cagccggcat
     2701 ggattgtgct cgtatcaatt gcgctcacga ctctcaggag ctttggggga cgatgatttc
     2761 ctatatccgg agggccgaac aggaaacggg aaatcattgt aaggtttaca tggatctggc
     2821 cggaccgaaa gttcgcatcg aatcagttct gctgcgctat ggggaagcga gggtcatgcc
     2881 cggtgagcaa atctttttgg tatcgggacc gatttccgtc tatcctgacg actataaggg
     2941 aaatatcgtc atttcgtgca gcatccccga agcctttgaa cacatggaag ccggacaacc
     3001 ggttctcatt gatgacggga aaattcgagc tatcgttgaa aaaatgacgc cggaaggggc
     3061 ctatttgacc attacctata ccaagccctt aggcggaaag ataaagaatg aaaagagcct
     3121 gaatttcccc cagacgccga ttgatgtgac cccaaaaagt tagactttta tttccgagca
     3181 acccgaaata gttgct
//
LOCUS       DAWDXZ010000164         3169 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6653_length_3169_cov_10.227360, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000164 DAWDXZ010000000
VERSION     DAWDXZ010000164.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6653_length_3169_cov_10.227360"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          706 a          869 c          829 g          765 t
ORIGIN      
        1 ggtatggagt ccgatatcgg tccaaacacc ttttcgtccg gcagccaagg ctctatacaa
       61 gtccaacagg gttcccgccg gtacggtata agctaaacat tcattcgctg tcaccttatc
      121 ggctaatttt ttttcggcac cgaagtgact ggtaatgatc gtccccaaaa ggcctttggc
      181 tgccagacga tcaccgccgc gtccccaacc gtcgctcgtc ccggtaattt tcactaacgt
      241 cagattgcgc ggatgcccct cttcggcata tcggtcatgc aaagccttga acaatccgtc
      301 cggtgtaccg aatccggctt ggccgctgac agccagggtc gtgtcattac ggaccaatcg
      361 cgctgcctca taaggtgtga taaattgtac catatgcccc ctcctgtcgc tttgatacaa
      421 accttttatc ttcttagaaa gaaagcccct ttaccataaa ggggctttgt gaatcgtaag
      481 cgattacgac aaatgtttta atcctttagc accgatatcg cggcgaactt gctggccgtc
      541 aaagtggatt ttatccactt tgtcataagc cttttcaatg gcatttttaa ggtccggtgc
      601 cacagccgtt acaccgagaa cacggccgcc atgggtcaca taggccccgt cctgtttggc
      661 cgttcccgaa tggaagacca tcgtatcgtc gctgtcgata ccgtcgaggc cgctgatgac
      721 atcgccctta tgagacgaag ccgggtaccc ggcagaggcc atgatgacgc agcaggccga
      781 agcatctttc cagtgaacca tatcaggcga caaagttccc ttcgtgcagg catacatgat
      841 ctgtgccaag tctccgtcga ggaggggcag gacagcctgc gtttccgggt cgccgaagcg
      901 gcagttgaat tcgacgactt tcgggccatc ttttgtaatc atgaggccgg cgtagaggca
      961 gccctgatag gtaatgcctt cggccttgag gccgtctacg acaggccgta aaatcgtgtc
     1021 ttctacgact ttttgaatag ccggcgtaaa gaccggagcc ggtgcgtaag cccccatgcc
     1081 gcccgtattg ggtccttcat cgttatcgaa gatacgctta tggtcctgag ctgaaatcat
     1141 gggcacaatt gtcttgccgt cgacgaaggc aagaaggctt gcttcttcac caaccatgca
     1201 ttcttcgatg acgatgcggc cgccggaagc cccaaagatg tgatcttcca tcatggcatt
     1261 gacggctgca acagcttctt cttccgtctg ggctacgacg acgcccttgc cggcagccag
     1321 accgtcagcc ttgacgacga tgggagcgcc ttgttcatga atgtaagctt tggcggcatc
     1381 gccgtcggta aaggattcat aggctgccgt cggaatccgg tatttcttca tgagattctt
     1441 ggcaaagacc ttggagcctt ccatctgagc cgctgcttta ttgggaccga aaacagccag
     1501 ccctttggcc tgggctatgt ccgccaagcc ttccgtaagg ggtacttccg gaccgacgac
     1561 gagcatatcg attccttcag aaacggcgta gtcggcaatg ccttcaagat cagacaagga
     1621 aatatcgacg catttggcga tatccgccat agcggcgctg ccgggaatgg cgtacaaggc
     1681 atcgacactc gggctctgag ctaatttcca agccagggta tgttcgcggc cgccgccgcc
     1741 gatgactgct accttcatta ctttgcagct cccttctcaa cctgttccgc cagatacgct
     1801 gcgatagccg tatcgtagac agccgtatga tgaaaggctt cctgcgccaa ttccaagcga
     1861 tattcatcgg aaaaggcacc gtctttttgg aggatggaaa tgatttcact atacttgtcg
     1921 ggatttgtta cgatggcgac atatttgtta ttcttcgccg aagcacggac catgcaggga
     1981 ccgccgatgt cgatgttttc aatggcttcg gccaaggtga cattcggttt ggcaatggtc
     2041 tgctggaacg gatagaggtt gacgacgacc aaatcgattg gcgtaatgtc gtgatccttc
     2101 atggctttga cgtgttccgg attatcgcgg acagccaaaa tagcgccgtg aatcttcgga
     2161 ttaagggtct tgacgcggcc gtccatcatt tccggaaagc ccgttacgtc ctgaacggca
     2221 atgaccggta cgcctgcttc ttttaacgta cgcatggtac cgcccgtcga aatgatttca
     2281 atgccgagag ccgccagggc cttgcccaat tcgacgacgc ccgttttatc agatacacta
     2341 atgagtgcgc gttttacctt catgctaacc ttcctcctat tcctgagttc ccatgacgac
     2401 attgccgcgg accgtcagtt tgtcgtcaca aaacagagcc gctgccttga tgtatgtcgg
     2461 atgttcttta gtcagaatcc gagccgccag cgtatcttcc gtatcatctt cataaaccgg
     2521 cacggaagac tgcaggataa tcgggccggc atccatatca ggcacgacga aatgaaccgt
     2581 gcagccggca atcttgacgc cggcttcaat agcctgacgc tgggcgtgga ggccgcggaa
     2641 ggacggcaag agggccgggt ggatattgag catcttgtgc tcataatggg agatgaaatc
     2701 gccgctcaag agccgcatga agccggccag gcagatgatg tcggcttcat aaggggcaat
     2761 ggcgtcgagg atgtatgctt caaaagcttc tttcgaatca aaggccttgc ggtcagctaa
     2821 gatgaccggt acgccccatt cccgggcccg atccatgacc ggcgcgccgg ggatatcgca
     2881 gaccaaggct ttaatatcag ccttgatggt accggcttcg acggcatcat ggagagcgat
     2941 ggcattggac ccgcggcctg aggcaaagat gaccatcttt ttcttagtca tggaacacgc
     3001 ctccccgcaa agtaacgggt acgtcggagt ctacgacctt gccgatgacg taggcttttt
     3061 catcgcggtc agccaaattc ttaagaatgc tgtcggcatc cttttcgtcg acgatcatga
     3121 tcatgccgat acccatgtta aaggtgcggt acatttcttt agcatcgac
//
LOCUS       DAWDXZ010000165         3167 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6659_length_3167_cov_6.653599, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000165 DAWDXZ010000000
VERSION     DAWDXZ010000165.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6659_length_3167_cov_6.653599"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          703 a          799 c          922 g          743 t
ORIGIN      
        1 gaagcgggta tcgtcacttg gattaatcct caggccatcc tcgacggaac gatgctcctc
       61 ggatcctttc aggtgacctt gagcgcatct cagcagacgc cctttctcag cggcgttctc
      121 ctcgcttcgt gcgtatggtt tattttcttg agcggcctcg tttctttgtt ccgcagccgc
      181 ttcagtccgc gcatcctgcg ggccatcaat attatctgcg gctctgtcat ctgtttttat
      241 ggcttgaagc tgctctttga ctttgccagt cgcctgagct gaggactttc atcggaagcg
      301 ggccttgggc gacgtcccgt aatactctct aaatgctttg tagaaattgc tgttattgct
      361 gtagcccagc atggcagcaa ttttttcgat ggaaagatcc gtcgacttta ggaggatggc
      421 cgcctttttc atgcggatgg acaggagaat ctctgaaaag gtgcggcctt ctttttcgga
      481 caagaggcgc gagaggtaga ccggatggta gccgaagcgg gccgccgtgc tcttgagggt
      541 cgccgtgtcg gaatggtatt cgatgtaggc catgatttct tctgtgaccg acgcctttcg
      601 ggccggctgg ttttgggccc ggacggcttc ggacatgtac atggccatgg agaggaagag
      661 ggctttcaag atggattggc tctggtccgt gcggcggacg tattccagta ccatcaagtc
      721 gaggatggac caaatcgggg aatcggctcc cggcgtgaag cggatgatgc cgtcggcata
      781 gcggttgacg gcgggctcta aaaagaagtg gagcaggtcc gtgtcggcgc cgaggacgga
      841 aaggtattct cggaggaagg tttctttctt gatgaggatg ccgacgatga cgatcgtttc
      901 gtcaccgtct tttttcaagg cgtagccgct gtaaggctgg cccaggtagc cgtcgccttc
      961 tttcatggaa atcctatttt ggctggaagc gctgagggct tcgtaatttc cccggtaggc
     1021 gaagtttaca aagaagaagt cctggcggtg gaaacgctcg tcgatgtggc tgcccgtaaa
     1081 ggcgcagagc atgatttcat cggccggatc gccgagccac tgggaaatga gttccgttcg
     1141 gccagatgac gggtcggggc gaaagtccca gttgaggagg gggaactccc ttcccagcac
     1201 ggtcagggcc tgttccaact gttcttttct gtcgttcaag agaatcacct gctgtcaaaa
     1261 cataaagaat ctgtcatcgg atgcagcctc cttcaggtta atatatacca ctatataagt
     1321 tatcatatgg cgctgtttcc agatgtcaaa cagggtacac tgataacaac agcggcgggg
     1381 tcacaagatt ccgaccgcca agggtatcta ttgactgagg aggtttttta tatgtatcaa
     1441 tttaattttt atatgccgac gaaagtacta ttcgggccgg gacggcttca ggatttgcac
     1501 aacgagcaga ttccgggaaa gaaagccctg atcgtcacgt cgaacggcac gtcggccaag
     1561 cgtttcggct atttggatgc cgttaagaaa gaattggatg cagccggcgt cggctatgaa
     1621 gtcttcgacc aggtgcgtcc caatccgacc gattacaacg tcatggacgg ggccaaaatg
     1681 gctcgggata cgggctgtga cttcgtcgtg gccctcggcg gcggctcggt catggactgc
     1741 gccaagtgta tcgccctgat gatgaccaac gacggccata tttgggatta cagcctgtct
     1801 gaaaagggcg gcaagaaaca gccggatgtt gatgcggcac cgacggtagc cattacgacg
     1861 tcggccggaa cgggcagtga agtcgacatg gcgtcggtca tcagcaaaga cgacgtcaag
     1921 gaaaagacgg gcatcttctt cccgtccatg ttcccggtgt tggctgtcgt cgacgccaat
     1981 ctgatgatga gcgtgccgcc gaaatttacg gcgtatcagg ggatggatgc ctttttccat
     2041 gctgccgaat cgatgatctg caagaacgaa catcctatgg gggaaatgtt tgccttgaag
     2101 gccattgaac tcgtcgcaaa gtatcttcct cgggcctacc gcgacggcag cgacaaggaa
     2161 gcgcgggctt atatggcctt ggccaacacg ctggccgggt actacatgct ctgcacgtcg
     2221 gaacacacga tggaacacgt catgggctcg taccacgatg atctcgtcca tggcgccggc
     2281 ctgattatgc tttctcatgc ttactacgat ttctttgccg aacgaaaagc ggctgaagaa
     2341 ccgatgatga agatggccaa agccatgggc gtcgccaacc cgacgtcggg caaggacttc
     2401 atcaaggcct tggatgaact cttagaagcc gtcgactgcc atgatttgcg catgagcgac
     2461 gtcggcatta cgagagacga attgaagacc taccctcagc ggatacacga cgtattgggc
     2521 ggcgatatta cggccgatcc agtgcccctt tccgatgaag attaccttga aatctttgaa
     2581 aaagcctata agtgaggcca tgctttcgcg gtaggcgagg ggaattttaa taagcttgat
     2641 acaaagagga ggtgtcttac ttggaaaagg atatgaaaac catgaccgag tgtgaaaaac
     2701 tcgacgccgg cctggcctat gatttttggg ataaaggggt caacgaccga aagttgaagg
     2761 ccattgccga aacgaagaag ctcaatgtca tcgattcggg cgatgaagaa gctatcgaag
     2821 cctgcctgcg ccgcctcttc ggatcctgcg gcagccatcc ttggacggca ccgggctttc
     2881 gctgtgacag cggcagcaac atccatgtcg gcgacaactt tttggccaac ttcaacgtca
     2941 ccatcttgga tatcggtccg gtccgcatcg gcgaccacgt catgattggt ccggggaccc
     3001 tgattacgac ggtcaaccat cctttgtcgc cgcggggacg gcggcagcat ttgggaatca
     3061 tgaagcccgt caccatcgga aacgacgtct ggatcggcgg caatgtgacc atcctgcccg
     3121 gcgttaccat cggtaataac gtcgtcgtag cagccggtgc cgtcgtt
//
LOCUS       DAWDXZ010000166         3153 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6680_length_3153_cov_5.952550, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000166 DAWDXZ010000000
VERSION     DAWDXZ010000166.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6680_length_3153_cov_5.952550"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1545..1554
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2576..2585
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          798 a          797 c          812 g          726 t
ORIGIN      
        1 agactgcggt tctttacagg gcatcgctgc cgacgggacg gatgacagtg ccggcttatg
       61 cgtcatcatg accgacgcgt cggtcgacgg gactatgatt gaaaaggcct tgattcaggt
      121 caaggccgat atggatacgg aacatttcac cttcatcgtc atggccaatg ggggagccgg
      181 cgcacagccg gtaggccatg atgcttttgc agggggaatc gaagcgttac tggtaaaatt
      241 aggctttcaa catgctttga aagcgtgcag ttaaataggg ggaagacaat agatgtttaa
      301 aagaagtgat ctcgtatcgg aagatgtaaa agctgtgacc aatgctcaaa aggcacagat
      361 tttgatgaat gccctgccat atattaaaaa gtacagcggt aaaacagtcg tcgtcaagta
      421 cggcggcaat gccatgatca atgaagattt gaaacggtcg gtcatgggcg atatcgtttt
      481 gttgaatctc atcggcgtca aagtggttct cgtccacggc ggcggcccgc atatcaaggc
      541 ggccttggat caactcggca ttgaatcgaa gttcatcaac ggtcttcggg tgaccgacga
      601 agcgaccatg aaggttgtcc aaatggtcct ggccggccag gtcaataagg atttagtcaa
      661 tctcatcgga tctatcggcg gcaaggctat cggcctctgc ggcatcgatg accagatgat
      721 caaggtccgc aaacagagcg atgaattggg ctatgtcggg cgcattacca acgtcgacgt
      781 atccatcatt caagacaacc tcgacaaggg atacattccc gtcatctcga cgattgggac
      841 ggatacggac ggccaggctt acaacatcaa tgccgatacg gctgctgccc agattgccgg
      901 ggccctcaat gccgaatgta tgctgtccat gaccaatatt gacggcgtcc tcaaagaccc
      961 gtctgatccc gattcactgt tgacggaaat caccctcgaa cagtcgcggc agttagaaca
     1021 ggacggcatc atcgccggcg gtatgattcc caaagttcag tgctgcaccg acgctatcaa
     1081 agccggcgtc aagcgagtgt tcatcatcaa cggcaccgtg ccgcacgcca tcctcattga
     1141 aatgctgacc gaagaaggtc tggggacgat gttcgtcggc cactattcct taggaaagta
     1201 aggctgttgg ggaaaacggt tgtcaacccg gattcgggta aaaaggagat aaaaacaatg
     1261 gcagaagatt ataaagctct tgataaagaa tatattgtaa acgtctacaa tcggatcggt
     1321 gccgtcctcg atcatggcaa aggagccact ttatatgatg tagacggaaa aaagtacatc
     1381 gacctcagtg ccggcattgc cgtcaatatt tttggtgtct gtgacgatga atggattcag
     1441 gccgtcgaaa cacagctcca taaattgagt cacatttcaa acgtcttcta tacggaaccg
     1501 cagaccatcc tggcggaaaa actctgccaa aagacgggca tgaannnnnn nnnnaggtat
     1561 tcttctcaaa ctccggtgct gaagctaatg aatgtgccat caagacggct cggaaatatt
     1621 ctcatgacaa atacggtgac ggccgccata cgatcatcac cttgaaaaac agcttccacg
     1681 gccgtacctt agcgaccttg acggcctgcg gccaggaatc acttcatcag gatttcggtc
     1741 ccttcgtcga cggtttcgtc tatgccgaag ccgacaattt tgacgacgtt aaacacttgt
     1801 gcgaaacgaa tcaggtctgc gccatcatga tggaaatggt tcaaggcgaa ggcggcgtcc
     1861 atcctctcga taaggacttt gtcaaagagg tcgcagccta tgccaaggct catgacatct
     1921 tgatcgtcgt cgatgaagtc cagaccggca acggccgtac gggctatctc tatgcctata
     1981 tgggctacgg cattgaaccg gatatcgtct cgacggccaa ggccatcggc ggcggcctgc
     2041 ccttgggcgt caccctcttt gccgcttcga cagagaaaac cttgaccgac catacccatg
     2101 gatcgacctt tggcggcaac ccgatctgca cagccggcgc catttccatc atcgaccgcc
     2161 tgacgccgga attgatggaa gaagtacaga aaaaaggtca gtatatcaaa gaagaactgg
     2221 aaccgtgcca acacgtcgac cacgtctccg gcatgggcct catgatcggc attgccctta
     2281 aggataaagc cctgagcgac gtcgtcgaag gctgcgtcga aaaaggcgtc ctggtcctca
     2341 cggccaatgg gctggtccgt ctcctgccgc ccctcaacat cccttgggat gacttgaaag
     2401 aagccgttgc catcgtaaag aaggtcatcg ataaataatt acatccatcg tttttgtaaa
     2461 gcagtctctg acggggctgc ttttttgctg aatattggga aggctcgttg ttgtataggg
     2521 ccgtcgtctt gctattgatc ggtgaatttc gtacaatatg gctataccgg taattnnnnn
     2581 nnnnncatat atgaaacatt taaagatagc ttacagtttt gacgtacaat attattttgt
     2641 cgacagcgac cgggacatcg tgcccatcga agagacggac ttcaccgatg ttgccgtagc
     2701 cgtcctgtcg gaccaggatt ttgattatat cgatgccatt gaggagaccg gtttcggcat
     2761 ccctatcatg gtcatcatgc ctggcggcga gaagctgccc gaatcgtata tcgataaggt
     2821 cgacgtcgtc tttacggaag aaatggtcaa taagagccgc tgcattgccg ctgccgaacg
     2881 gttggccggc cgttatgaac agacggtgct gccgccgttt tttgccgacc tcgtcgacta
     2941 tgtcagtgaa aagaatacgc cctttgactg cccgggccac caagatggct tatttttcaa
     3001 aaagcatcct gccggccgct acctctacga tttttacggc ccccatattt tccagtccga
     3061 catttgtaat gctgacgtca ccttaggcga ccttctcatc catgaagggc cggccctgga
     3121 agcccaggat tttgcggccg aagtcttcca cgc
//
LOCUS       DAWDXZ010000167         3152 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6683_length_3152_cov_6.672263, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000167 DAWDXZ010000000
VERSION     DAWDXZ010000167.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6683_length_3152_cov_6.672263"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    314..323
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          686 a          834 c          811 g          811 t
ORIGIN      
        1 ctgaaaagta gggtccgggc aggacaagtc accggcgacg ccgagataaa atcgaacgcc
       61 ttcgggcgta cctaaaatta aatagacgaa gtggatgccc ttcatatggc gaaaggttcc
      121 catgacgttt tccatggcct cccggcgggg cgccttttct tcataagtaa tctcatccag
      181 acggaacaag gacagcttgg gaaggctcaa agccgggccc aagtccttcg gaacctcgcc
      241 gcctccggcc gtatccggaa cgccctgctc caaaaaggcc atggcctgtc gggacgctgt
      301 caacgccgat tcannnnnnn nnnattcaac ttgattttgg gatgcctcat cccttatttc
      361 ccctaatttc atcagttcac ctcaatgttt gacgaatttt atgtttctgt gccacgcagt
      421 cggaatcttt aaattcagcc cccgtgccca cagcgtattc gacggcgtcg ggcgcgactt
      481 tccgcaggaa gatcatgtgg tcgtcgtcat cggacacatc ggccgtatcg attcggcagc
      541 ggacgtactt cacggccttt tgcggcagtt ttccttccaa ggcctgaggc tctgccgtgt
      601 aataaatctc aaaatactct tcatcggcat agcaaaattc agcccacgat tggtaaggca
      661 cgccttgccc gatgcggaca cagaagttgc cgtcgacgtc gatatcgcct aagaaatacg
      721 ggaacaaggc ttcgccgctg tcatcgacgt tgtcattgac gattttgacg tccttcccgc
      781 ccttacggct tcgcaggagg ccgaaggcaa cgccggtctt gccggtcagc ttctggtcga
      841 gttcctccgt ctgtccttcc tcttcttggc catcaaaacc taagggcatg tggagaacca
      901 gcatgccttt ggcgtctttg ccgccggcat gagccgaaat ttcttcttct tctttttgga
      961 tctgcgcctc aaagagttct tttacgatag acgagcggca cgagttgccg gccagtaaaa
     1021 tatggagttt atccggctct tcggtctgat gtttaaaggc cgtcttccaa gcttcaaaga
     1081 agttggtcac gccccgtgaa acgaggctgc gaataacttc ttttaattta tcgctgtcga
     1141 cgatgagctt gaccggaacg cggttttgat tgtcttctcg cttcttctgc tctttttcgg
     1201 catcgttttt cttgtctttc cgtcccgttt tactgccgtc ggtaaacaac agaattgtcc
     1261 attctttgtc ttgccactct ttgatttcat cgggccgttc ttcccagatg ggacgcatgg
     1321 cttcagccag gcatttgcgg ttcatgaagg aaatctgcgt cgcatccctt tcggccagga
     1381 tgagggtctc cgttccggcc ggagccgtgc agccgtgagg cagggcgatg ggaatggcgt
     1441 tctcccgcat agtgcctaaa ttggtgcgga atacttcaaa agccaggacg ttgaggatat
     1501 tttcgccgcc caaataggga tcgccgccgc ggccgaattg atggatctcg aacttatgac
     1561 ggcgcggatt ggcggccttc ttttcgatgc caaagtcaaa gtccgtcgta ccgccgccaa
     1621 agtcgaagac gccgtaagcc gtgatgtcct cctttttagc cggttccagc ccgtacgcct
     1681 gcagggcagc cgccgcgtag gctgcaggtt cactggctcc ggcgtagact aagaaccaat
     1741 ccatgcattc ttcgtccgct aagatagccg gcggcaggga ctttttcaag ccccgctcaa
     1801 agctcttgag caatttagtg cggacagcca aatcgtagtt gaccgggaag gacaggatgt
     1861 agtccaaata gatgccgtgg cgcatattat tgatgtagag gcctaagtag taggcgtaga
     1921 tttcgatggg atcgaggtcg tcgtcatcga gttccaagta gggcttgagc ttccatacct
     1981 tgcccttgcg gtctttcaaa agcagacgac ggtctttttc atgggcccat tgcttcagtt
     2041 cactgaagac ggcataatag acggaactgt ccacatcctg ttggagcagg gcctgttcgg
     2101 cctgatgagc tacggtcaca tcttcccact ccgtaaaggg acggccgatc cgctgtccat
     2161 aggccgatgc aaagcgctct aaatcgcgga gttcgacaac cgtcgggttt tcgtaatctt
     2221 tctccttgac ggccttagaa aagtcgccgg tgccgatgcg caggaggcgg gccccgtcct
     2281 caccgcggca gacgacgacg gaactcttgg tgccgaagtc gatggcacag gtcccgttgg
     2341 ccacgacgtc gagctggggc ggccgggccg tccagacctg attcgccgtc agcggtacgg
     2401 taatcgaatc ggcttcgggt gcttcaaaga gttcccaatg gcccttattg atgtccgaca
     2461 aggccgtatc ggcatagggt tccagattgg cccgtcggta gtcgcattcc aataggtcct
     2521 tccgcagcga cagatactca ccttggtact tggtttgacc ggacttgagt tcttctatga
     2581 tgcccggtaa gttaaaccgc cattgaaaga ccgtctgtcg aaaacggccg tggaggacat
     2641 cgtctataaa ttcatcacga cgaaaagata ctcgattgtc ctcaaaagtc caatcaaggt
     2701 aggcatcaag gttttgatag tatgataaaa gctgattata aattgcttct tcttcctcac
     2761 taagtccttc cggtactagg cccttttgca gccatgtcaa taatgtctta gctggagtca
     2821 agcccgaact atccttgcag tccaggcgag cgatcataat cattaacata tagatgtctg
     2881 aaacgtaatc tttctgtgtg ctttccttat tgatatcatg ataccgtagc cgcggacgag
     2941 aaccgcaatc ataatctgta tcgacaaaaa aacttctact ccaagaattg aaatttttag
     3001 gaaaattaga aattgtacca tcttcttgaa tataaggatt gccatttccc tttttaaagg
     3061 ttttccctgc ttctcctggt gatgctagga caaactgggt gcggtcgacg gggagttcgt
     3121 gtggggggtg ttccgtggcg atgagtcggt cg
//
LOCUS       DAWDXZ010000168         3148 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6690_length_3148_cov_10.124475, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000168 DAWDXZ010000000
VERSION     DAWDXZ010000168.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6690_length_3148_cov_10.124475"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    689..698
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2045..2054
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          729 a          750 c          928 g          721 t
ORIGIN      
        1 cggctgcttt aattcgaaaa gcgtcgacta ttccggtcaa tgggtcggct acggaaaaac
       61 ggggttcccg aaagaaatgg actgcgtcta tgatgtcaaa attgaaaaga ataacgatgg
      121 ctatatcgtc agcttagaac ggagtaattg ggaattaaaa gatattcccg atccgggctt
      181 aatcgacagc atcgtcaacc aggacaatca gcagtatgaa tggacgacga tgaaggaaat
      241 gaacctcaag gcagaggaaa aagacaacac ccttacgacg acgacggaaa atcctctgac
      301 cttcacctat gtagagaagg acggaacgct ggaactgtcc tttaaagact attcccatca
      361 ggatgaacag gtaacggtac agctccataa ggccgaaaag ggcgaagtag aagactttaa
      421 gaaacgttcg aaagaagata ttttaaaagc tatgacgccg gacaatcagc attcttttaa
      481 ggaatgatcc ggtaagacag gatccataac gacataacag gccatagaaa aaggactacc
      541 gattcacgag gatgaatcag tagtcctttt cgatgagaag gcgtcaggcc tcatcggaca
      601 ggatccgttc ttttaattct aagtagcggg tcgtaaaata cggtgtcagc tgggcttcgc
      661 gcaggtcgtt gccgaagggg cggcggctnn nnnnnnnntt ggcggactct gaatccgttt
      721 ggcaacggca aagccggcca ggaggcgcca ccaccgttcg aggtcgtcga tgaattcctt
      781 ggctgtcgga aataattggt ggacgtcgac agtgcagccg atgttctttt ccagggttcc
      841 gtcgagatac caggtcagga tcgattcggg cgtagccctt tgccagcgtt cgacgaaggc
      901 ggcgaagagg tagtcgtggt aggggtaaat caaagggtcg ccttgtccct tggtgatatc
      961 ctgtttgttc gacagttcgg cgctggggac gatgtcgatg ctgccctgag ggacgacttg
     1021 gcggccgaag accttgtcgt tgaggtagtg ggccagttcg tagacctgat gcttccagag
     1081 gtcagccgtg gcggctaaat agccggcgct gtcgccgtag agggtggcat agccgacaga
     1141 cgtttcggcc ttattggcgt tgcacgtgaa gacgccgcca aaggaggctg ccgcagcggc
     1201 caagatgcgg ctcgagcggt cccgggcctg gatgttttct tcgatgaagc tggtcaaggc
     1261 gaggcttccc ttttcctttt cgccctgatg gaggatggtt tccctgaact gcttgcgcgt
     1321 cagctccagg ctgtcctgga cggggatgac cgtatacagg cagccgatgt tgtcggccag
     1381 ttctttggcc aggctcttgg tcgtgtccga attgtagcag ctgggcatgt tgaccaagag
     1441 gatgtcctcc ggcggcagga ccgtcgcata gagggcggca ttgacggccg aatcgatgcc
     1501 tcccgatacg ccgatgacga ctttatggac accgatggca tccatgaagt gcctgaggcc
     1561 gtagtggagg ctgtcgtaaa tatcttcaat ctgggacttt tccggctgca cctgagacgg
     1621 agacgtaaag gtctgcgtcg tttcgtcaaa gtcgacgagg aggagctctt ccgtaaaggg
     1681 cttgcaggcg ctgtggtggc tgccgtcttt ttggtaggtg ctgctggcgc cgtcaaaggt
     1741 atagatgttc ttgccgttgt tctggatgcc tgtacagttg acgtagaccg ccgggcagtg
     1801 aatcttgtcg atggcctggc cgaagaggcg gtggcggcgc tgggtttttc ctaaggtaaa
     1861 aggcgagttt gaaatattga cgaacaagtc gatatcgtac atcttgccta aatagtgcat
     1921 cggtttgaaa ggatagtttt catcccagct gtcttcgcag atgagggggc cgatgcggaa
     1981 cttgcggccg tcgtcgaagg aaatctggac gggcgacaag aagtcttccg ggaaggcgtg
     2041 ccgtnnnnnn nnnntcgctg aattcccggt aattgggaag gagggtcttg atgtagaagg
     2101 ggtagggcga acgggcaggg gacagccatt ggccgttgta ggcgatgaac atggcgttat
     2161 attttcgcgt ccggccgtcg aggttgaccc ggtccgtttc tttggctacg ttgccgaaga
     2221 tgatggccat gccctgagac aggtcgcgga tttcttcgcc gaagcggacg cattcgtcga
     2281 tgtaatcggg ctggtcccag atgtcgccga tgaagtagcc gggaatggca agttccggga
     2341 agacgacgat gtcacagcgg gctgccttgg ccttggcgat gaagtccttc atgaccgccg
     2401 cgttctgccg aggattgccg ggatagaccc gggtctgggc gagtgctatt ttaaacaagg
     2461 aaaagcctcc ttttcatagg tgagaatcta tatactacct catcttatca taaaacggta
     2521 gctgcgaaaa ggttggccgc ctgcggtcgg cgaaaaaaac atgacagaat ggcgtccttg
     2581 cggtatactt aatgtattat caagattgaa ggaaggtctt tcatgaaaac gctgctcacc
     2641 attttgaatg cgaaatttat tcagtcgtct ctggccattc gctgcctgac gacggcctgc
     2701 gcccaccgag gcgtcgccgt cgatgcggct gaatatacga tcaatcaaaa tccctacgac
     2761 atcctgcgtc acattgcagc ctttaaagcc gacgtcatcg gcttttcctg ttatatctgg
     2821 aatattgaaa tgacctgcca tatcatcacc ttagtcaaaa gcgtcttccc cgaaacgaag
     2881 atcttcgtcg gcggtcccga agtctcctat acggcccgcc aggtccttct tgactgcccg
     2941 gatatcgact acgtcctcca gggcgaaggg gaaatcatgg tgccggattt cctgacggct
     3001 ctcgaaaagg gggaagacgg gacggatatt cccggcgttc tcggccgcgt tgacggaaag
     3061 attcaaggcg atgaaacctt tcaggaaatc gacgacctcg acagcctgcc cttcccctat
     3121 gaaaatcagg actttcgcga cctcgacc
//
LOCUS       DAWDXZ010000169         3145 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6696_length_3145_cov_8.502265, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000169 DAWDXZ010000000
VERSION     DAWDXZ010000169.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6696_length_3145_cov_8.502265"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    639..648
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          848 a          760 c          583 g          944 t
ORIGIN      
        1 tgtgctgcag cttccatccc acgccaatca ttgccagttg caactacaac cggatccatt
       61 ccattcataa tgcctttatt actggtagcc gcccggaagg ggtccgcttc agcaaaagca
      121 taggcatcta tcattccatc aacaacagag tctccaccga tagcctcttt agaaaaagta
      181 gcagttgctc gtgccaaccg atacgcagct aaattcgata atatacgcaa cttcaccgtt
      241 cctttagtaa ttttttcaat caatggcgct accgcttctg ccatcaagtg tactacaacc
      301 atcgggccta taatcgtttc aataacacgg acttccacat ctttagctcc acctgtaatt
      361 ttcaccaata ctggatcttt tgcattagct tgatcaataa tttctttttt atggtgtaaa
      421 atagactgac atgcaaaata cggatttgat acccctacaa cttggacttg ggcaatcata
      481 atatttcccg tatagctggt atgaaaccca ccactagatc ttgccatttt agctgcattt
      541 gaagctgcag cgacaataga tgcttcttct acaaccatag gaatgaggcg ttcctgtccg
      601 tcaatgataa aattcatagc cacgcccata ggcagttgnn nnnnnnnngt tgataagtac
      661 caattacatt ttcaatgcag tgatccgcta ttgtatcgga aagtgctccc ggctttgtaa
      721 tcgtatttaa ttcatcagcc gttaagtctg aaaattcagc aacttccatt gcccgttgtt
      781 ccattgtgag tttgtgaaat cccgaatatt gatttgtttt catgataatt tctccttaac
      841 taaagtttaa aatttataac tttttattga ttagtcctcg tccacctcaa tatacattct
      901 caggagagct gcttccatac attttcccaa tgtatcaagg cgcagtgaac gtgtagcacc
      961 gccatcaaga gcgtggtgca tgacaaaatt aaatccgtcc aactgaggca catcataacg
     1021 aatgacatct ccgaatacca agccctcaaa atggcgacga acattttccg gcgtcaattg
     1081 tgcttccata aatttataat atttaggttc tcttgcatag atgcaaatat tagacgtatc
     1141 acttttatct ccacaacggc cgtgagcaat atcttttaac tgtactttca tgtttttata
     1201 cctcctcaac atatgtcgta agcttaatag catctctcgg gattaacgtc ggccagagag
     1261 caaagacttc actcggacga ctgcgcccgc cgaagaagca tccctgtgcc ggtccattca
     1321 ggttgctgac agctgcgact tccggcgtaa gtttcaagcc ttcctgcttg ttttcagtca
     1381 tgatggcata gcgaaggacc acttcattga gatcggcatt aagttccggt gccagagggc
     1441 cgtgaagagc gtttacacca agataactta tatgcttacg aacaatatgc gcatttttca
     1501 tcgctaatcg cttatcgaga atatcagctg ccattttggc tttgtctaaa gctcgcggcc
     1561 aggaatatgg caaataagca atcgtgcggt acccttcaag ataacctacg cagagcttta
     1621 aggtatccgg ggctggggtc ccggtgacgc ctcggacggc cacatggtcc cgttcaattt
     1681 cttccaatgt aattttgctg aaatcagcaa cggtatcggg cgtaatgtat tttgacggat
     1741 catgaatttc ataaagcagc tgttccttaa cggactgggg tgtaacgagg ccgccggtgt
     1801 tcttagtttt cgtcaagata aggctgcctt tttcactgac ttcaacaata gggtaaccca
     1861 ggttttccgg gctgggaaca tgttcccaat cgtaatcata gttgccgccg gttgcttggg
     1921 caccgcattc acaaagatga ccggctaaaa tccccgttgc cttagccgtc cagtcgtctg
     1981 atttccatcc gaattcatac atgagcggag cttcaaacat agctgtatcg gtcgatctgc
     2041 ctaaaataat gatatcggca ccgtcttcca agcatttgac aatgggttcc acgccataat
     2101 aaacattcgc attgaccata ttcggcaaaa tacgatcgat atcttcgccg gtatccatat
     2161 gtttcatggt aatgcctttt tctctgagct gaggaatcat ttcacgcaca tcatcaccaa
     2221 aaacgtaacc tacttttaag ctgtatccgc cttcgcgaat aacttctttc gccttttcaa
     2281 cgcaggcttt aacgttcatc ccaccggcat tggtgatgac tttcgttcct ttttgcgtaa
     2341 tatagggcag cattcctttt aaggctgtaa taaaatcacg tgcatagcct gcttccgggc
     2401 gcttttcctg ctgtcttctc atgatcgata aagttaattc agccaagtaa tcgcagcaga
     2461 tataatctaa attatcctct ctggccatcg tgataggggc atcacttaaa tccccccaaa
     2521 agccttgtcc accgccgatt cgaattgttt tcatacgggt ttacctcttt tctttgtatt
     2581 gtatacatca cagtatcgaa atgttactga cacgcctttc tatatgaaat ttttatgcca
     2641 atcaagaaaa gacgccgaaa aaagaagtcc tccatcttta gagattggga ttctagcagt
     2701 gctatcacct ttaattaaaa ttttacaaaa aaacaccatg acccattcag gcacacaact
     2761 gtatacctaa ataggtcatg gtgtttacat agtatacgat aaattacgtg aaacatgttt
     2821 acatagtata cgttttataa ttcttcatca aacccatatt gcttccactt cttataaaaa
     2881 gtgcggcggc tgattcctag cgccttcata gctttcgttt tatttccgtt cgcctccaat
     2941 actgcctgtt gaattcgctt cttttcaatc atttgaagac cggatttcaa ggaataatca
     3001 cggctatctt cgacagtata cgcactaaaa gaagtattca cacggtcttg atcctcatgc
     3061 tgcagaaggt catccggtaa gtgttccaac tgaatgattc cttcattgca aacaatgacg
     3121 gcatattcca tacaactttg cagtt
//
LOCUS       DAWDXZ010000170         3134 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6718_length_3134_cov_5.127314, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000170 DAWDXZ010000000
VERSION     DAWDXZ010000170.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6718_length_3134_cov_5.127314"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    291..357
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1357..1366
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          694 a          834 c          648 g          881 t
ORIGIN      
        1 gcccggaagc cagtagaagg tatgtccctg ggcctgcaca tcccagaaga caacatcgaa
       61 ggacggagcg atatgatctg caatattaag ctccacacat atctccccct atcgtcggaa
      121 tacaaacgat actgcatccc cggtattgcc ggtttgcagg tccgcgactt cggccttgag
      181 tttctcgatc cgcaggcgct gctcttcgtc cgcgagtccc tgccggcaca tctcgtcata
      241 ttgcttaatc atcgagttaa gtgttgccat tgcccgggcc tgggccatca nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttg
      361 tccgcaaact ggacctcaaa ctctatttcc ttcgtcgagt ccccgtattt cttgcgacgt
      421 accaacatgc tatggtcttt ttcgttatcg acatacatga tcttctgggc tcgaataata
      481 gccgcatact tgatacagat attttcccat agaatatcta tcgccgattt ttcctccaga
      541 gcctcaacta gctcccgcgt ttcgtccggc agatacttag catatagccc atgcacaagc
      601 gcattctgat tgcccttatg gctctcaccg gagccgacgg cgttattatt gtaataaggt
      661 gcacccgttt ttttgtgtgc acccttctta cgggtccatt tgtatcgccg ctgccacgac
      721 ttgacggtgt tgagcgatac gccgtgtttt ttcgcgatat ccttatattt catgccctta
      781 acataatcgt tataggcttt ttcctgcgtt gtcacatact caccacctcc ggccgtcgtt
      841 gtttgttccg taatccacgc ccttacttga tgactctatt ccggctgcca ggatacaccg
      901 gcccatgatt gatagacgtt cggcggtcta ttggcttgcg ataagactta caaggcgtag
      961 gcccgccgtt aagatctgcc tcgataacac atgccccgcg cttattgtgg gtacagtcat
     1021 acgatctaca catcgcgcag cctccttccg ggcacgaaaa aaggaaccta gcacgttgtc
     1081 taagttcctt aaatattttt tgtggcagga ccggaaggac tcgaaccttc aaccgctggt
     1141 tttggagacc atcactctac caattgagct acagccctat gcaccctccc ctgttgtaaa
     1201 gattgcggat aagggaggga aaaagccggg gccggacttg aaccggcgac aggtgagggc
     1261 ctataacccc tcaagctcta gccaactgag ctacccggcc atgacggccc ctaagggcct
     1321 gtgacagtgt gtgggatggc ggcaatcagc gcttaannnn nnnnnnttgt gtactggtgc
     1381 gtatcacgag ccgccgctca tccccaactc tctacaatac cagtatactc cttttagagg
     1441 tcacattgag ttaccttttg gaggaaatcc ttatgtatag ccgtgaacga ttggagtgcc
     1501 cgcccgtgta ccctgaaaat atacttaggg ttataatgtt gctcctctgc gatatcatcc
     1561 caatccatgt tattgatata cctcgcgtat aagatatctt gataggtagc ctcgggcagc
     1621 atgtcaatgc ggtttttagc tgctatcctc atatcaataa ggacgtccca ctcgtcgttg
     1681 acccggtcaa gatatcgctc tagcttgata tacttatcag caatatcaga gatcttagac
     1741 ccggacactc gctccgtcag gctagcagcc ttaagcgtca atatatccgc ctggacctca
     1801 agcaattccc gtttaacctg ttgtaactta tagcgctggc gcctgatgcg ccccaagtac
     1861 tctttagctg tcatcaagtc acttccttac agcaacacca taatataatg ggggctttgc
     1921 gcgaccttgt agccccattc ctcgaccgtc tgcatgacgg cagtgtcgac gtcctggtcg
     1981 ccagtctttc ttattcgagc cgagtagtac ccttttttaa tcgcattttt cactttgttc
     2041 tcaacgtact gttttgtcag cattatcata ttcccacgtc cctcattgtt cccggctaag
     2101 caagtccagt gcatacataa cggagtctgc tacggattcg agattgtcca aactgtcata
     2161 caattcgtcc gtatcccagt ccatcaagcc ggcgtcgcca agctggtccg ttagtgtcat
     2221 cgcttcgtcg cccaaagccc cgtaaaacgt ccgtttcatc gtcgtgcgcc cgatttcttt
     2281 atcgccttct tcgacaacta tggtgtatcc attcttattg ataattactt ttatttccat
     2341 gtcattacct cctaattaat caccataagg ggtatatttc aagccctcta tacggcttaa
     2401 caaaagctct acctcatgtt cttccagcca tccaataacg tcgtctgtga tttccgtgga
     2461 atagcatatt tccccatgtt ttaatacggc cagttcccac atgccttcgg ggccgccata
     2521 tgtacgaggc ccccggctaa cgttagcgcc gtaattgttg tcaaaccgga aaatattgat
     2581 ctcgttgccc gtatcctggt caatatatgc gtgtattgac ttatatctgc caaacttttg
     2641 agggatgata tcctttggca gatctcccac gatcgccagt ccctgacagc cacacggctg
     2701 gccgttggca tcccggacca gtggcccggg aacgaggaaa tcagtacgtt gtggaagagc
     2761 ctctgcaacg agtcgagata caacataata ggtgccttct tccggatacg gcacattttc
     2821 gacggctccg gcaaaatcct gataggtaat tgtaatacca tcaatatacc ccgcgatagt
     2881 cgactcctga tggcatctag ggataggcct gtcagatacg gggatatctc taagatgacg
     2941 gtgcccgtcg aaaatattaa ttatgtgggg cgttaaatta ataattttta ccttgtattt
     3001 atcagggtca aacggtttca tcctttttaa ttgcccattt tcaaattctt gctgcatttt
     3061 taatcctcct gtttcgttat aatttttcaa aacgttgttc catctttttt agttcgcgct
     3121 ccaggctatt aata
//
LOCUS       DAWDXZ010000171         3130 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6725_length_3130_cov_8.868618, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000171 DAWDXZ010000000
VERSION     DAWDXZ010000171.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6725_length_3130_cov_8.868618"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          767 a          753 c          823 g          787 t
ORIGIN      
        1 agggggaatg aacatgagta aaacccatat tgatgaattg gtgtctcccg attattttaa
       61 tcggaaagag tgtgaaggct gcggctgctg catcagtatc tgtccgtacg acgccttgtc
      121 catggaaacc tgtgccgatg gggttgaacg gcctcgtgtc gatatgacca agtgccttcg
      181 ctgcaatcgt tgcgtcgatg cctgtgaaat ttacaagtgc tacggcccga cttcggaagc
      241 gatatgattc gccgttattc gcggccgaat aaatactttg tgtcgtagct taaatccata
      301 tactgggcaa tggtctcgtc gtcgaacccg tattcttccg acttgcgagt caatttgccc
      361 cacttcatct gcatcttttt cttgggtggc aagggttctt cgtattcgat gcgaagttcc
      421 tgcgcttctt gggagcggtc ctgtactttt acgaataagc ccttatcaaa ggtcaactcg
      481 tagattttgc ggaaggcatg gggaagctgg aagccgaagg ggacgaagta tttgtcgatg
      541 aagccgttgg caatcaagat ggagccgctg taatcgatga cgtgttcgag gtcatacttg
      601 aggtcgcctg ccggtgagtt aaacggttcc ggcaagacgc cgtcgatgga cgggggctcc
      661 atgccgttgt tgttggtcaa taacttatga agggtcagca ttttttcgtc gttaaatccg
      721 aagacggctg tatatccgcg caggcacgct tcgttataca tgatgggatg aaggccgaaa
      781 tcttcgatat taaagaagtc atcgaccttt tcgatggcga caatgctgag tttgaaggtg
      841 gcaaaatcga agccgtcaaa aaattgagcg cgcatgataa gtctccttat tcgacaaaga
      901 tgggcgtgtt gttttcaaag cgggcgattc tggctaagga ttccacacgg aaaccggctt
      961 tttcaagggt ttcccgaccg gtctggaagg tcttttcgac gacgatgccg atgccttcaa
     1021 cggtgcagcc ggcctgagcc gcaatctgcg taagaccgac ggctgcggcg ccgctggcca
     1081 aaaagtcgtc gatgatgaga accgattccc cggcacggag atagtctttg cagaccgttg
     1141 ccgtgtactc ttcttctttc gtataagagt aaatttttgc cgtatactgt tcacccttgg
     1201 tgagcagtga tttttttttg cgggcaaaga cgaccgggca attgttcatg gcaagggatg
     1261 ccgcataagc gacggcaatg cctgaagatt caatggtgat gatgcggtcg atgtgaacat
     1321 ccttgaaacg gcgggcaaat tcctggccga catgattgat aagagccgtg tcgatttgct
     1381 gattcaaaaa gctgtcgatc tttaagatac gattgtcaat aaccagaccg tcttgtaaaa
     1441 ttcgctgttt caattcttcc attacttccg agtcctccct ggtgatgaga aatggtcaac
     1501 cttgtctact ataatagtac tagtctaata cgatttcata ggcaagtcaa caaaattcta
     1561 agtttactta tggatttaaa acgggtatac taaggttgtc tattgataag tatctatctt
     1621 tgagggaggt tatgacatgc agtctattcg acgtttgtat gttgccaaga aaggtatttt
     1681 tgctgatgag gcaaagcgat tactcactga tttacaggaa aacctgttga taaaggggtt
     1741 aactgatatt cacattttcc accgctacga cgtatccggt ctcgatgatg cggcttttga
     1801 agcggctaaa aatatgatct tctcggaacc gcctgtcgat gccgtttacg atacactgcc
     1861 gactcgtgaa ggcgatacgg tcctggctgt cgaatacctg cccggtcaat atgaccagcg
     1921 tgccgattca gccgttcagt gcctgcagat gctgaccatg aaaaatgaca gtctcgttcg
     1981 gacggcacag gtcatcgtcc tgtccggcga cttatcggct gaagatatcg atgctgtcaa
     2041 acattactgc attaaccctg tcgaagctcg tgaagcttcg ttggaaccgg ctgaatcgtt
     2101 ggaattggct tgggaaaaac cggctgacgt ccctactgtc gaaggcttta tctccatgga
     2161 tgatgcggcc ttgaccgacc tttcggcgca gatgggcctg gccatgagct tcgacgattt
     2221 gaagttcgtc cagacctact tccgcgacga agaaaaacgg aatccgacga ttaccgaaat
     2281 ccgagtcatc gatacgtact ggtcagacca ctgccggcat acgacgttca tgactgaact
     2341 gacggacgtt tcttttgaaa aaggaacctt tacggctcct atacagcggg cttatgaatc
     2401 gtataagacg acgcgggaaa acctgaaccg caagaaacct caaaccctca tggatatggc
     2461 aaccatcgcc gtcaaggaat tgaaagctgc cggaaaactc gataatctcg acgaatcgga
     2521 agaaatcaat gcctgcacga tcattattcc cgtcgatgtc gatggcgtcg aagaagaatg
     2581 gctgctctta tttaaaaacg aaacccataa ccacccgact gaaatcgaac cgttcggcgg
     2641 cgctgcgacc tgccttggcg gttgtattcg cgaccccttg agcggccgct cctacgtcta
     2701 tcaggccatg cgcgtaaccg gtgccggcga tcctcgtcag gctgtccagg atacactggt
     2761 cggcaaactg ccgcagcgca aactgacgac cggtgccgct aaggggtaca gttcgtatgg
     2821 caatcagatc ggcctggcta cgggtgaagt caaggaatac tacaacccgg gattcgtcgc
     2881 caaacgtatg gaaatcggtg ccgttctcgg tgcggctccg cggaagaacg tcgttcgcga
     2941 agaacccctt cccggcgata tcatcatcct cctcggcggc aagaccggcc gtgacggctg
     3001 cggcggcgct acgggttctt ccaagaaaca taccgttgaa tccttggaaa cgtgcggtgc
     3061 tgaagtccaa aaagggaacg ccctgactga acgaaagatt cagcgcatgt tccgccgcca
     3121 ggaagttacg
//
LOCUS       DAWDXZ010000172         3129 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6726_length_3129_cov_9.936890, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000172 DAWDXZ010000000
VERSION     DAWDXZ010000172.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6726_length_3129_cov_9.936890"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2494..2503
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          812 a          822 c          702 g          783 t
ORIGIN      
        1 cgagtcctgt atttatagta aggagtgctt ttatcatgtc cgagtcacga gaaaagacga
       61 tgcgcctgac acacatcgcc ttgttgattg caatgaactg tatctctgct tatctgatta
      121 tccccctgcc cttttctcag tcgcccctgg ccctgcagac gctgatcgtc aacttagtgg
      181 ccttcctctt accgccaaaa gacgctgcca tcaccatgct ggcctatatc ggcatcggcc
      241 tcatcggcgt ccccgtattc accggcggca cctccggtcc gggtaagatg ttcggaccca
      301 ccggcggata tatctgggct tatgtcgtcg ccgtctacct catgtcgatg ttaaaaggga
      361 aaacatataa cttcaagcgc tattccctcg tcgccatcat catcggaatt cccgtcatct
      421 atcttggcgg cgtcctgcag ctccacggcg tcactgaaat gccttggagt gctgtcatca
      481 ccagtggcgt cctgcccttt atccccttag acatcgtaaa aagcatctta gcagccgccc
      541 tggcaaggcc cttgctcgat attacggcca taaaaaatgc agtgccaaaa aattaggcaa
      601 aaagcccatc gcgagatggg cttttgtcgt attagaaatg gcttttagca aaagttccga
      661 tttcctgcag ggacgcaacg gaagcatcga gatccggtag gcagagggca aagtcatggg
      721 gcatatcagg atagacggtc atcgtcactt caacgccgtc gtcgacggct ttttccataa
      781 acttctgatt ttccgtcagg aatatttcat gaccgccggt ctgaatcagc atcggcggca
      841 gccctttcaa atcggcatga atcagcgata acctcgggtc agagagggac aatgtcccgg
      901 cataagacgg ctctttgaca gccttattga gcggcgtccc acggcctaaa atcttatcct
      961 tacggtcatt ataggtccgc gaagccgcag ccgtactgaa atccgtccat ggtgactgta
     1021 ggactaacag tcggggcaag gcattttgat ggtcccgcaa ataaagcaca agggcaacgg
     1081 ccagatttcc gccggccgaa tcgccgacta acacgatatt ttcagcctta atgccgctgt
     1141 caaggagata gttataagcc gcaatggcat cgtcaagggc agccggatag atgtagttcg
     1201 gcgctaacct ataattaaca caataagcct ctttagcatc catcaaggcc gcctgcttca
     1261 gggccatgag tcgatgaccg tcactcatgc cgaggacata accgccgccg tggagttgta
     1321 agagcacctt atcggaatca gaattcgtat tcgtatactt ctcgacgacg ataccactga
     1381 cggtcacatc ctgtcgctgc cagccatcag gcaaaacgaa aggcttctga ttgcggccgg
     1441 taaagaggtc gcctgcggtc ttttcaatat attgctgctg agcctgtcgc tccaagatca
     1501 ggccatgttg cggcaggtaa agcccctcgg ctgaagcagt caaattcatg gcaatcaggc
     1561 agacaccgat cgctgaaatc ttcagtacct tcttatgcaa cataacaaca caccctccct
     1621 ccgtgatgaa aaaacgattc acatatgttc ttattttacc attgaacggc cgctctaagt
     1681 caaggcggaa agcggagaac caaagtatgt ttccacaaaa aaagaagtac cgcaccgcta
     1741 tgggcgacac ttcttttcct ctttttagaa aaacagagca gcaacgaggg ctgtcgtcaa
     1801 aaattacgct gtaatttctt gaatacggcc gatgagtttt tctgcatact cccgagcagc
     1861 ctgtttctga gcctcaacag ccatttcatc atcgcgagtg gtatagccga caccatggac
     1921 gtagagagca ccctggtaat caagaccgca aaggttcgcc gttgccggaa atatttcaag
     1981 catcttttgg atatcgccaa tggcgccttc gccgccggta taggcttctg ccggggcccc
     2041 tgtcgtaaag gatacgagca gctttttccc accgagtttc gccttagagc catgggaaaa
     2101 gccatgaagg aagacttcgt cgagccactt cttcatgagc cagggcagag aataccacca
     2161 gaagggaaac tgccacacga tgaggtcggc ggccagtaag gacttctgtt ccgcttctac
     2221 atcgatattc gctgtcgggt agagctgatc cagcttgcgg atttcagcat ccggaagggc
     2281 cttttcaagc tcgtccagaa tcaccttatt ggcaacggac gtcgagagat cgggatggcc
     2341 ggaaataatc aaagttttca tattatcttc gcctttcatg gataaaagaa gtacgaaatc
     2401 tagttactga ctgtttttca cagcccattc cttcagttca ccggcagata cgttgttcgg
     2461 aaagcgtttt cccacagcga cagaggcccc cgannnnnnn nnnctgcatg ttcttaccgg
     2521 aatctccaat aggactcatg ccggacgtag caaaaggaac gaccttttta ccactaaaat
     2581 catagctttc cataaaagta tcgataatcg atggttcccg ataccaccag accggaaagc
     2641 ccacaaagac cacgtcatac tgactcatat ttacgacctt ttcggcaata gccggacggc
     2701 ttcctggatc cttagattct atggaactgc ggctttctga attctgccag ttaaggtccg
     2761 ctgagctata gggcacttcc ggttttattt caaaaatatc ggctccaatc gcttcagaaa
     2821 gttttacggc aacctttgcc gtcacaccgc tggcgctaaa ataggcgact aatgctttat
     2881 tcatgataga ttcctcctct attacatcga ataggatact tgattggtca atgatagatt
     2941 tttcatatca atcatggcga tcatatcacc acttaagatc agtctatcat tcatgggcat
     3001 tcatgtctaa tacctatcgc aaatcgccag aaatgcgttt atcgtatttc tatgcaataa
     3061 atcaaaggta gcgaaaataa atatacctcg atttcaattt ctagaaatgt tagtatcaaa
     3121 gtagtatca
//
LOCUS       DAWDXZ010000173         3096 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6791_length_3096_cov_9.694508, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000173 DAWDXZ010000000
VERSION     DAWDXZ010000173.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6791_length_3096_cov_9.694508"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2246..2255
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          806 a          857 c          787 g          636 t
ORIGIN      
        1 taaacacaat attccgccgg cagaatggac gtacagcaac attgaaaaca tgacccgtca
       61 gcaaaaggcc ctgggcctgt cctacgactg ggaccgcgaa gtagcgacgt gccatgaaaa
      121 gtattataaa tggacccagt ggatctttga actgttctac aaacggggcc tggcctaccg
      181 caaagaagcc aaggtcaact ggtgcgatac gtgcaacacc gtccttgcca acgaacaggt
      241 cattgacggc aagtgctggc gctgcgacaa tcccgtcgtc aaaaaggacc tcaaacagtg
      301 gttcttcaag atcacggact atgccgaccg cctcttagac gacctcaaag aactgccggg
      361 ctggccggaa cgggtcaaga ccatgcagaa gaactggatc ggccgcagtg aaggggccga
      421 attctccttc gacatcccgg aaatcggcaa aaaggtaccc atgttctcga cccgtgtcga
      481 tacgatcttc ggcgttacct acatcgtcct ggctccggaa cacgaatacg ttgccgacct
      541 catcaaaggc aaggaaaatg aagccgaact ccgggccttc attacgcgca tgaagaacat
      601 gagcgacatc gaccgcacgg caacggacgc caagaaggaa ggcatgttta ccggggctta
      661 tgccgtaaat ccggtcaaca acgaaaaggt ccccatttgg atcgccaact acgtcttggc
      721 cgactacggc accggcgccg tcatgggcgt accggctcac gaccagcgcg actgggaatt
      781 tgctaaagaa tttggcctgc ccctgcgcct cgtcgtccag aataaagaac agaacctcga
      841 tcttgcgacc atggaagggg cctaccacga accgggcacc ttggtcaatt ccggtgaatt
      901 caacggcctg accgacgaag aaggccgcaa agtcatcacc aaatggctgg ctgacaaagg
      961 ccttggcaaa aagaccgtaa actataagct ccgggactgg ctcatttccc gtcagcgcta
     1021 ttggggcgct cccattccca tcatctactg cccgacctgc ggcgaacagc tcgtccccga
     1081 agaccagctg ccggtcaagc tgccggaaga cgtcgaattc acggccggtg ccgtatcgcc
     1141 cttggcgacg agcagcagct ttgtcaactg cacctgcccg aaatgcggcg gcccggcgaa
     1201 acgggaaacg gatacgatgg acaccttcat ctgctcgtcg tggtacttcc tgcgttatac
     1261 cgacgcccgc aacgacgaac aggcctggga tcccaagaag ggcaatcact ggatgaacgt
     1321 cgaccagtac atcggcggca tcgaacacgc catccttcac ctcatgtact cccgcttctt
     1381 catgaaggtc ttccatgacg ccggcctcat cgaagcgaaa gaaccttttg aacgcctcct
     1441 gacccagggg atggttctcc tcgacggcag caagatgagt aaatctaagg gcaacatcgt
     1501 atcgccggaa gatatcatcg ccaaatacgg tgccgatacg gcccgcttat tcatcctctt
     1561 tgccgctccg cccgaacgcg atctggcttg gtccgatcag ggcgtcgaag gatcgtaccg
     1621 tttcctcaac cgcgtatggc gcatcgttca ccagttccgg gatatggaaa aagccggtga
     1681 ctcccataag gcctataccg aaggggaaaa agaactgcgc tttgaagaat tcaaggccat
     1741 ttccaaggtc acggaagacg tcctcggtaa agacggcgaa tacggcctca atacagccgt
     1801 cagctcgatc atggaattcg tcaacgccat gcacgcctat gccggcgcca acacgacgat
     1861 ccatgacgac gtagctcttg aagccaataa gaacctgctc aagatgctgg cgccctttac
     1921 gccgcatatt acggaagaac tgtggcagat ctgcggcttt gaaggcagcg tccaccagga
     1981 atcctggccg gaagtcgata agacggccct cgtcgtcgat gaaatcgaac tgccgatcca
     2041 ggtcaacggc aaagtccgcg accgcctgac cgtcgccgtc gatgccgata aggaagccct
     2101 cgaagcacag gtcctggccc tgccgcatat taaagaattc atcggcgata aaaaggtcgt
     2161 caaatttatc gtcgtaccga agaaaatcat caatatcgtc gttaaataaa gaaatgtgaa
     2221 tatacttaac aaaggagggg actgtnnnnn nnnnnctgcg ccgtcttggt gccggtcatt
     2281 cacaaagacg gggaagaaca tttggttttt gaagtccgca gttctaagct cgactggcag
     2341 cccggagaaa tctgctttcc cggcggccgc atcgataaga ccgacaagag tcccttggcg
     2401 gcagccctgc gggaaacgaa ggaagaactg ggcgtttctg ccgaccacat ccacctctgg
     2461 ggccccctgg actacctgga atcgcccgtc ggcgtcaccg tctggccctt tgccgcctat
     2521 atggatacgg cggattttac cttgagcacc ggtgaagtgg atcacacctt tacggttccc
     2581 ttgaaatggt tcgacgacca tcagccggaa atcggcaaca tcgagctggc aacccggccg
     2641 gcaccggact tcccgaagga cctggagatg cccatgcgag gccagtggcg gccgcgccgg
     2701 acctaccagg taaagattta tacgtacggc gattataaaa tttggggaat tacggcacat
     2761 atccttgaca actttagggg tatccgtgct ataatacaac cgtaagcttt cctaagtatt
     2821 tgtaaccagt atagttaggt ataagaataa aaaccgacag taaaaatgag acgtaccgtg
     2881 tcaaggctat tttctccttt ttgccttgct agtaccaaaa agtcttgtta atactttaag
     2941 atgagaaaat ggaatggtta tattgtgatt tatagcactt ttgctattga tttaacttgt
     3001 gaaaagttgt acaatgcact tgtaagcact gagaccgtgt gaatatatat ccaagctgtt
     3061 tttttgtatc aaccggacaa tgtattgtct cagatt
//
LOCUS       DAWDXZ010000174         3076 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6832_length_3076_cov_6.425025, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000174 DAWDXZ010000000
VERSION     DAWDXZ010000174.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6832_length_3076_cov_6.425025"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1500..1509
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          751 a          740 c          776 g          799 t
ORIGIN      
        1 acgtgaggtg ctgacatgga aaaaattaaa tgtaatttta aacgagttat ccagccgaac
       61 ttatatcaag atggcccgaa agggtttgga ccgcctccgg tatggactta tcaaggtaca
      121 gtagacgggg attactgcac cttggaaata tatgacagtg ggaccgacga atacattgcc
      181 tatacggtta ctatacatat tcgcttttct cgatatatgt atcggtatga cagcggatgc
      241 tcggaagaaa acctgtctac cataccttcc gacgttgtcg atgtagttgg tccttggctg
      301 gctgaactgg gatatcccat caagtcggaa gcggaaatcg actaaatgca aaagacgccg
      361 tccgttatgg atagcgtctt tttttctgtc ccatatcagc cggccgtcaa aaaaacggca
      421 aaaaattaat tctaaaatac atcaatatat gttatagtta actgtacgcg actctccaaa
      481 aaccgcataa atagcgtagt tgtattaatt tattgctaat taagagagta tcgcggtata
      541 atatagagat aaatatttct ctttatgaat aaggatgatt gctatggaaa aagttgggct
      601 cttggccggt atcggtaagc ttcctgtcga gtttatgcgt gctgcaaaac acctgggata
      661 tgaagttgtc gtcatcgccg tcgtcagcga tgtcgaaccg gaactggcag cggaagccga
      721 tgcatactac gatatcaacg ttgccaagtt ggataaaatc attaagaccc tcaaaaaaaa
      781 tgacgtctcg aaagttacca tgatcgggaa ggtcaccaag gaaattctct ttaaagggct
      841 taaatttccc gatttgcggg ccattaaact gttggcggcc aaagacggcc ttgaagtcgt
      901 cgaccagacc ttgtatttga aggatctcat gccggccatc ggtgtcttgt cgcgccgcaa
      961 gccgacggaa ggggaagaag acgatatccg cttcggtttc gtgacggcta aggccatggg
     1021 cggtatggat atcggccaga ccgtcgtcgt taagcataag gccgtcatgg ctgtcgaagc
     1081 cattgaaggg accgatgcct gtattcgccg cggcggggct ctcggccgtg aaaaatcggt
     1141 tgtcgtcaaa gtggcaaaac ccaaccagga ccctcgtttc gatgtgccgg ccgtcggcag
     1201 gcagacgatt ttgtccatga ttgaaagcaa ctgcaatgtc cttgccatgg aagctggacg
     1261 gacccttttt gtcgaacaag atgaagtcct gcgcctggcg gacagccacg gcatcgttat
     1321 ctgtgctgtt gataagaact tttgacggga gatcctatga agattatgtt ttctgccggt
     1381 gaagcgtccg gtgacctcca cggcgccaat ttggcacgag ctttgaaaca acttgcgccg
     1441 gacatcgaac tgctcggtat gggcggtgaa aagatgagag acgccggtgt cgatatcgtn
     1501 nnnnnnnnnt cgataatttg ggagtcatcg gcatcggtga gattatccgc aagattcctt
     1561 ttttctttcg cctccgtgat tttctagtcg atacgatgaa gaaggaaaag cccgacgtcc
     1621 tcgtttgcat cgactatccg ggctttaata tgcgccttat aaaggccgcc aaagctgccg
     1681 gaattcctgt catttattat attctgccga ccatttgggc ctggcataag agccggggga
     1741 agacgattgc aaaatatacg gatttggcta tttccctttt cccttttgaa gccaagctct
     1801 atgaagatat agggacgaat gtcctctata cggggcatcc ccttttagac agcgtccatc
     1861 cgagcctgag ccgtcacgac gcttttttga agtttggcct ttcggaagag aagcgaacga
     1921 tccttctcat gcccgggagc cgtgtccagg aagtgacaag cctgctgccg tgtatgttgg
     1981 aagcggcgaa acagatagag cgtgactacg gagacctgca atttctcatc ccgcgggcta
     2041 cgaccatcga tagagccatg cttgatgata tcattgcctc ttccggcatg actgtcactg
     2101 tcggagaagg caatgtctat gacatgatga atatcagtac cgctgcgatt gcagcttcgg
     2161 ggacggcaac ccttgaaacg gccctgatgg ggcttccgac ccttctcgta tatcgcgtca
     2221 atcgcctgac ctattggctt tccaagattc tggtacatat tgaaagtatc ggcctgccca
     2281 acatcatcat gggccgcagg gtgattcccg agctgtggca ggatgatgtg acgccggata
     2341 atattgctga cgtcgtcctt cctatgctgg acgacggacc gcgccgggcg aagctctgtg
     2401 aggacctttc tgccgtgcgc cgtacgatgg gggaatcagg agctgtgatg cgtacagccc
     2461 aggctgtctt aacttttgca agagaaagag gtattcatga agcagtacaa tagttggcaa
     2521 ggttataaac gattattaac tttcatcaaa ccctataaga cacggctcgt tttcgccgtc
     2581 atctgtatgg ctctgtccgg ggcgagcaac gtcgtcgttc cctggctaat caaggacgtc
     2641 atcgacaagg tattggccaa taaagacatt tataccttga accttatcgt catcggcatc
     2701 ttggtcctgt ttttactgcg cggcgtcttt tactttggcg aacgctatct catgagtttc
     2761 gtcggccaga aaatcgtcaa tgatatccgc gaaaaactgt accgccattt gcagaccctg
     2821 tccctgtctt attttgataa gcataagacg ggcaatatca tgagcaacct gaccaatgac
     2881 gtcacggccc ttcagacggc cattgccggc aacctcattt ctttcgttca ggaagccgtc
     2941 atcctcgtcg gttctttggc atcgatgatt ttcctctatt ggaagctgac cctcctgacc
     3001 ctggtcatcg tgcctctcgt cgtttttacg atcaacttct tcggctcccg cctgcgccgt
     3061 gccggccacg acgtac
//
LOCUS       DAWDXZ010000175         3058 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6867_length_3058_cov_9.928738, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000175 DAWDXZ010000000
VERSION     DAWDXZ010000175.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6867_length_3058_cov_9.928738"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          622 a          793 c          867 g          776 t
ORIGIN      
        1 aagataatgg gcttatcata gagagacagc aaaggcctta aggatacgtc gccgtcgcgg
       61 atgagttctt ccaaatattc cctgcgcagc tgggcaaagg cgaacttccc cgtaacgcag
      121 acggcgctga tgatcaggca gatcagaaaa acaagaaaac attggacgat gagttgtgct
      181 tccatcttgc cggcccccta ctcgttttta tagccgaatt cgctgagggc tgccgccttg
      241 ttgcgccagt cccgcttgac cttgacccag atgtcgaggt agaccgaaga accgaggaga
      301 ttttcgattt cctgacgggc caggttgccg atttctttca ggacagcccc gttcttgccg
      361 atgacgatgc gcttttgcga atcccgttcg acgtataccg tgacccggat atagacctta
      421 tggttgcccc ggtctttcat ttcttccgta aagacggcga tggcgtgggg cacttcgtcg
      481 cgggtgcagc ggaacagttt ttcccggacg atttcggcca ccaagagccg ttcgggctgg
      541 tcggtgatca tgtcgtccgg gaagtatttc ggtccttccg gcaagatctt gcgcagttct
      601 tcgacgacgg catcgacgtt gtctccgttt aaggccgaaa tgggaatgac ctgggcaaag
      661 ggatagagtt cggcatattc ggtaatcttg gtcaggatct cttccttgct catgaggtcg
      721 actttattga tgattaagaa gacgggaacg cctgtatcct tgattttttc gaggacgaat
      781 aagtcgccgg gccccttctt ttcattgccc gagatgagga acaggacggc gtcgatatcg
      841 cgcagggact ggtaggccgc ttcatccatg aattccccga gcttatgctt gggcttgtgg
      901 atccccggcg tgtcgaggaa gacgatctga caagcctcgt ccgtatggat gcagaggatg
      961 cggttgcgcg tcgtctgagc cttatcggag acgatcgaga ctttatgctt gataatgcgg
     1021 ttgataaggg tcgatttacc gacgttgggg cggccgacga cggcgacgaa tcccgattta
     1081 aacgattcca taacgataat aggtcctcct tataattctt cgccttggcg gacgtaatcc
     1141 aggcgctgta agataaattc ttcttctttg cgcatttcgg ccttgtcttc cggttccatg
     1201 tggtcgtagc ctaagatgtg cagcatgccg tgaatggtga ggtaagccag ttcccgttcg
     1261 aaggaatggc cgtattcatc ggcttgttcc tgtacttttt caagggaaat gatgatatcc
     1321 cccaaaaggg ccgtgtccgg gccttcatag ccgtcttctt ccccttcatt gagagcaaag
     1381 gacaggacgt ccgtcggccg gtcgatattg cggtactgtt tgttcaaggc gtggatgtag
     1441 tcgttgtggc acaagagaat gctgatttcg gcgttcggtc cgagaccgta aaccaaggcc
     1501 gcttcttcac agacccgttc gataactttt tcatagtctt cctgttcgaa tgaaggatct
     1561 tcatagttga tgttgatatg cacgagtggt accccctttg atacagtgtc cccctgtcct
     1621 ttcgggcaga tcatttatct ttataataat tttcataggc tctgataatg cggccgacca
     1681 tgtcgtggcg gacgacgtct tcgtcgctga agtggaccat gccgacgccc ttcacgccgt
     1741 cgaggatctt ttcagcctct gtaaggcccg acatgcggcg gtcgacgagg tcgatctggg
     1801 tggcatcgcc gttgacgacg acccgcgaat gattgcccat acgggtgagg aacatcttca
     1861 tctgttcccg cgtcgtattc tgggcttcgt cgaggatgat gaacgattct tctaaggttc
     1921 ggccgcgcat atatgccaaa ggcgccactt cgatgatgtt gcggtcgatg agtttttgga
     1981 actggtcata gccgaacata tcggccaagg cgtcgtacaa ggggcgcagg tacgggtcga
     2041 ttttttcctg caggtccccg ggcagaaagc ccagcttttc gccggcttcg acggccggac
     2101 gggtcaggat aatgcgttcg acttcacgat tctttaagta aaaagcagcc agggccacgg
     2161 ccagataggt cttccccgta ccggcagggc cgatgccgaa ggtaacgagg ttcttccgga
     2221 tagagtcgac gtattttttt tgtcccagcg tcttgggctt gatcaggcgg tggcgggcag
     2281 aaatgccgac cgtatcttca tagagggcat gcagctcttc gccccgcccg gctttgatga
     2341 ggcccgctga gtagcggacg tgctgcgtcg tgagataggt attttcccgg aacaagtagc
     2401 gcagggcgcc gaagacgtcc aggacttgtt cgacttcctg ctcactgccg acgatcatgg
     2461 ccgtgtcgcc gcggacggca atgcggcagt ccggaaattc acgctgtatc gtctttaaaa
     2521 attcatcttt catgcccagg acggcccggg cttcacgcaa atcggaaaat tgcagtttct
     2581 tttctaccaa aataatctcc tttatgtcat gaatttacgg acagctttgc cgtgcagata
     2641 gtcgcacaag gcgccttgac gggtgtcacg gcgttctaag gcttcgtcga tcatatcgcg
     2701 gatgcgccac cagcccatgc cccagttggc aaaatccgtc gcctctttcg gcgctctgcc
     2761 gatgaggcgc tggaagacga tgtccggccg gctgtattcg aggaaggcta cgacgcggcg
     2821 gacgtactct tcgcaagtca ccaggctgat ttcgcccttg gcgtaccatt cggccatggc
     2881 cgtattcttt atgatgtaca gggcgtggag cttgacttca tccgtgccgg ccgccgacac
     2941 gagtttagcc gtttcgatgg tgtcttccat cgtatcccaa gggaggtcga ggatgacgtg
     3001 ggtgcagacc cggaaggggt aatcgcggag catcatcatg gcatcgacgt attctgcc
//
LOCUS       DAWDXZ010000176         3039 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6895_length_3039_cov_7.119638, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000176 DAWDXZ010000000
VERSION     DAWDXZ010000176.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6895_length_3039_cov_7.119638"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1110..1119
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          730 a          687 c          736 g          876 t
ORIGIN      
        1 gaacattgta aaaaagcaac ggaactctta gcagaaatcg gtgctgatga taaatttgtc
       61 catgctgtcg tctgccatgg ctatggcatc tgtgtcgatg tggccccgga acatatgatg
      121 gaaaaagtcc tctatgctgc cgatgaactg acaggcctta tttgggcagc tgccaagatg
      181 cgcccttcaa agagcacgaa agacatggaa gtaaagagtt taaagaaaaa atttaaagat
      241 aaacggtttg ctgccggctg ttcccgcgaa gtcatccaaa atggcgctga attattgggc
      301 tgggacttgg cagaactgtt tgaaaaaact attttggcta tgcgctcctg tgaagacggt
      361 gttcgcaacg aatgccggga attgaccgga gtggatgaat aaaaggagga atctactatg
      421 aaattacgca aaacgattat ggcagctgtg gctgcggcta tgttgacagt gggtgctgcc
      481 ggtgcttctt tcgcagcagg tttgggtacg attgacgctg ccgctctctt gcagaatcat
      541 ccgaagtacg cgaagaccat ggccacctgg caggccgacg taaaatcggc tcaggacgat
      601 ttccagcggg atctgaagaa gacgaacgat caaaaagctc agcaggcctt agtcgaaaaa
      661 tataataata aattgaacca gcagcgtatc gctttattcc agccgctgga aaaagatatt
      721 cttgataaaa cccaggctgt tcagaaagaa aagggcttgg attacgtcgt attgagcggc
      781 tccgtcgttg tcggtcagag ccaggatatt acccaggacg tcgctgctcg cttaaaataa
      841 ggtcagcatt taccgtctga gggaaggtaa gatccatcat aaaatatata aagacgcagc
      901 tcgatggagc tgcgtctttt tttgtcagaa cctgtctgtg tgtgtcatcg cgagtgaagg
      961 acttttcata acctcgatag tttgaaaaaa ggcagtcatt gccgaaggag gggtaatttg
     1021 ttagaaaaac gtttttcgga tttttgacgg catattcgat ttgtctgaag cttctcgtga
     1081 ataaaggtac ttataatgat aggtctgaan nnnnnnnnnc aaaaaaacgc aaaggctgtc
     1141 ttaagccgtt atattttatt ctcagcttat ttcacgtatg atgagggtac gaaatgagtc
     1201 attcggaaac agaagaaaag gagaataagt atatggaaat cattgctatt gtattgagtt
     1261 tgttcttatt agtcacattc gcttaccgcg gattttcggt cattttattt gcacctattt
     1321 tcgcattgtt tgcagcgagc ttttccggtc tgccaatctt accggggtac acggaactgt
     1381 ttatggcgaa agccgttacc tacatcaaat ccttcttccc ggtctttctg ctcggtgccg
     1441 ttttcggcaa gattatggaa gaaacgggga tggcccgcag tattgccttg gaaacgattc
     1501 gactcatcgg gaaagataag gccatcttcg ccgtcgtctt tgcctgcatg attctctgct
     1561 acggcggcat ttccatgttt gtatgtgctt ttgccgttta tccctttgca gcagctttat
     1621 tccgcgaagc cgatattcct aaacgcttga ttccggcctg ctttgtagcc ggtgtcttca
     1681 cggctacgat ggactgctat ccgggatcgc ctcagatcca gaatattatc ccgacgattt
     1741 acttgggaac gacgaccttt gcagctccga tcttaggttt aatctgcggg accttgctga
     1801 ttatactctg ctttatttat cttgaattcc gtcgtaaaca actggctcgt tccggtgaag
     1861 gatacggtaa ccataccctt aacgaaacgg tcatcgatcc ggaagccaaa cttccgccct
     1921 ggcagttgtc cattattccc cttatcgtcg tcttagtttt aaacttctat ttctctatga
     1981 ttttccagtg ggatgaatct ttattgagcc ccttccgcag catgaatgtt cctcttctgg
     2041 ctaaaggtgt tcacaatgtc gtcgccattt gggctctcgt cataggcctc ttggcagcca
     2101 ttatcattgc cagtgcaaca ggacgtaaat tcatgtccaa aaatacgagc atcaaagcgg
     2161 ctcttaatgc cggtgcttta ggatctctcc tggctattat gaatacggct tccgaagtcg
     2221 gttacggcaa cgtcatcgca tctttgccgg gattcatgga aatcgccgat gccctcttag
     2281 ggattggtca gggcatgccc cttttcaacg ttgccttaat ggtcaacatc ttggccggta
     2341 ttaccggttc tgcttcgggc ggcctcagta ttgccctcga tatcttcggt cagcacttct
     2401 tggctgaaac ggcagctgtc ggcattcctg ctgaagtcgt ccaccgtatt gccgctatgg
     2461 catcgggcgg tatggacagc cttccccaca atggcgccgt catcacgacc ttggctattt
     2521 gcggtctgac tcatcgtgaa gcctataaag atatgtttgc cattacgatc ttaaaagtta
     2581 tcattgcctt cttcggtgta ttcttctata tgctgaccgg catcgtataa aaataaaaag
     2641 ttcgtgaaaa aaggtactga ctcagacgag gcagtacctt tttcggctat tcttttagtt
     2701 ctgttgaaca tactcttccg attcgggtca ggatgttcaa ggcatcttcg cgctctttgg
     2761 gcgtaaaatc tttgaaaacc aagtcattta attcttcgat tttctgctgc accggttctc
     2821 ggatggccat gccggcttcc gtcggtctga ctcggatgct gcggcgattt ttcgggtcca
     2881 ggatgcggtc gataaggccc cgtttttgca ttcggtcgag gacgccggaa atggttgagt
     2941 tttcgactcg cagaatcgtc gcgatttcct ttggcgtcag ggtttcattt ttccaaagac
     3001 aggccagtac gccgtattga ccgggggtaa tgccgaatt
//
LOCUS       DAWDXZ010000177         3039 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6892_length_3039_cov_24.066689, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000177 DAWDXZ010000000
VERSION     DAWDXZ010000177.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6892_length_3039_cov_24.066689"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          681 a          763 c          727 g          868 t
ORIGIN      
        1 taattccaat cagcagttga actgctgtga tttataagtt taaccgaggt catttaagcc
       61 tcggaattag ttgtaaatat ttgaaaatta agggatggaa atacttgtaa aatccttgga
      121 aatcagtaac ttaaagtaaa aaatacccct attttttgct tgttatgatt cccaaggaca
      181 aagagtatag actaataaaa atctatatgt atatctgcga tatgtacgaa caaagcctca
      241 aataccattg ccaaagatac agcaataatt cgaaaccgtt gttctccgac gaggaaatcc
      301 tcacgattta tttctttgtc ggtcatgagc agaagtacac cctcatcaag gatattcaca
      361 acttcgctaa agagtacttg cgcgactggt tcccgaacct ggtgtcctat cagacattca
      421 attaccgtct caacaggatg gccggtgctg ttagggaact gtcctcgcag ttattaagga
      481 tgttcaggcc ttctgactgt caggatgata ccgtgattgt tgactcgatg ccgataatca
      541 catgctgcgg aagaaaccgt acaggcaagg tcgccagaga tatcgctgac aaaggatact
      601 gttccaccaa gaacctgtac tatcatggac taaagcttca catggtagga tatcgcagga
      661 aaggccatct tcctcatccg tgccagatag cgctctctcc tgcctccgag aatgacctga
      721 aggtcttcca gagcgaatgc atgccggatc ttttcaacaa gaagatcttc gctgacaaga
      781 tataccgaag caatgactac tgggagcagg aaagacgcga taaggccaat gagttctacg
      841 ctcccgtcaa gtcaatcaaa ggggctcatg aagaagagaa gcagagaagc aaggccgctg
      901 atgacattta ttctcaagcc gtttcatctg tcagggaacc cattgaagcg ctattcagtt
      961 ggctgaacga aaaaacaaat attcaaagag cttcaaagtg ccgctctact tgcggactgc
     1021 taattcatac gatgggaaag gtagccatcg catttttata ccttattttc aactactgat
     1081 tcaaatcagt aataaaaaat gtttttattt tttattagtc tgtgtcgttt tatattggtc
     1141 gctctgttta taccaatatg tagcttcgcc ccgccattcg ttccgatctc tatataaata
     1201 cccggttgcc aatattgtca aaattgataa tacggttaga aatacgattg ttctcagaac
     1261 gataggtggt tgtttgagat agcgtatctg ggttttcatt tcccataatg tattgttggc
     1321 gagcttttcg aatttcgtcg tatcaacttc gttcttgaat tcggataaag actttttgaa
     1381 acttagaact tcagtaatta ttgttcgatt ctccttgtcg atcacctcca gtctccccgc
     1441 taactgcgcc agcaacgcgt tgtatctgtc ggtaaattcc tgcatctgct cggtttttgc
     1501 tccggacaag gccgtattcc gttgcagttg ttcgagctgc tgtgcgatct gcccttgcga
     1561 gcgttccgcg gcctctatca gccgttcgat ccgttccagc tgcgtattct cccaacctgc
     1621 gttccctacg ccgcctgctt tggcgttttt aatggctttg acaagtgttt ccttgaggtc
     1681 ttcgtacatc tcgattgaga ggtcgatctt atttttgtcg ttcatggctt tccggtttta
     1741 aggttatatt ttcattttgg ctcttctttt ctgttcttcc atcctgcggc ggcgttccat
     1801 tgcggctatg gccatcgctg cagcagcggc atcgagcagt gccgtgattc gtgctatatg
     1861 ctcttcggga ctttctcccg gcttgcgctg caactgtgcg gccgatactc cgaccggcga
     1921 actggccggc ggaagcggaa tcgcgtcgat ctttccgccg gggctaggcg ccgcaggctc
     1981 ttcgcgcgta ccgccgccca tgccgccgaa caacccggca aatgccgaac ggtaattgcc
     2041 tgccgccgcc gagaggtttc cgaggagctc ggcccgatgc agcgcctgct gttgcaggtg
     2101 ggtgaaccgg ttgtcgagtt tcgtaaagct gaatgccctg tcgatcttcg atccggagaa
     2161 ctcgaagccg tttttcgaga agaggatacc ctgcttgcgg tcggtatttc cgcaatactt
     2221 atagcggacg ttgatgccct gttcctttaa tttaccttcc aactcgttcc agttcttgca
     2281 tttgggcaga cagcctttga tcgcatcgta gatttcgtat ctggtcttgt cgggttcgcg
     2341 cagccgctcc cgccgtacgt cgtcctttcc cggtgcgaga tacaatccgt acttctcggt
     2401 cagctccctg cataccttgg cgtttcggag cttgatgttc ttgtccgaaa tggtctgtcc
     2461 gtcatttccg acccggttgt agaccagatg acagtgcgga tggggctggt cgaggtggcg
     2521 aaccaataga tactgcgtat cggtaatgcc cattttctgc atgtactcct ttgcgatctg
     2581 cgtcatcagg gcgtcggtca tccgcggagc gtccttgggt gaaaaagaca gcgagatatg
     2641 cccgacggtg tttttcagcc gcggattcaa taaggtctgg tctttgaagt cctgcaccat
     2701 gtccttcact tccggaggca taatgccctc ggcttccaag atccgggatt cctctttcat
     2761 cacgtagcct accgtccccg aaaaagacga tcccgatatt accttaccga ccatcgcata
     2821 tctgtttcag gagtttttcg atggccgtga cgatggtcgc atgccgtact ctgacagccg
     2881 ccagaccttt ggcattggcc agccgggtca gttggttcag gttgcgggca atgcctttga
     2941 gttgggccat gaaatccagc tgttcgcggg tgatccgtgc tcttaccgtt cctcggtcga
     3001 tcagctggcg caggacttcg gccggcggtt gcccggcct
//
LOCUS       DAWDXZ010000178         3036 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6901_length_3036_cov_7.170413, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000178 DAWDXZ010000000
VERSION     DAWDXZ010000178.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6901_length_3036_cov_7.170413"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2562..2571
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          713 a          727 c          782 g          804 t
ORIGIN      
        1 cgtttctacc gggcacatgc ggccatagtg cgaatggtgg acgtcgcgga cttcaaagcc
       61 tgcgcgttca cgggacaaac cgccgggacc aagggcgctc agacggcgtt tatgtgtaat
      121 ttcagccaag ggattggtct ggtccatgaa ctgggacagc tggctggaac cgaagaattc
      181 tttaatggcg gctacgacag gacggatgtt gatcaggacc tgcggcgtaa tgctttcggc
      241 atcctgaatc gtcatgcgtt cgcggacgac acgttccata cgggtaaggc cgatacggaa
      301 ctggttctga aggagttcgc cgacgcagcg cagacgacgg ttgcccaaat ggtcgatatc
      361 atcgacagtg ccaaaaccat ccatcaaatt gaggaggtag ccgatgtgag cgatgatgtc
      421 ttcgcgggta atcgtccggt gatcataagg cagatcgctg ttattgcaga tgaccttata
      481 gacagagcca tcctgctttt caacgaaagc ttctactaaa ccgccgccgt caccgagggg
      541 ggcatcggct gcgatttcac ggtggaatac accgctttcg cggatttttt caacttctgc
      601 cgagccgatt tccgtatgag catcgacgat gatttccccc gtttccggat tgacgatcgg
      661 ttcgtgaagg gtcaggccga agaggcggcg ttcccaaccg agtttcttgc cggctttata
      721 acggccgacg cgggccatat cgtaccgacg cggatcgaag aacaaccctt caaagaggtt
      781 gcgggcgctt tcaacagtcg gcggttcacc ggggcgaagt tttttataga tttcaattaa
      841 ggcttcgtcc tgattttccg tcgtatcttt ttcaaaagta gccttcaggc gtttgtcgtc
      901 gaaaaagagg tcgaggatat ccgaattgga cgcccagccc agggcacgga tgagaaccgt
      961 aaccggaatc ttacggttgc ggtcaatgcg ggcatagata atatcgttag catcggtttc
     1021 gagttcgatc caggcgccgc ggttcggaat gaccgtcgaa ttgtaaaggc ggatgcccat
     1081 cgggtcaatt tcacgaccgt agtacacacc gggagaacga acgagctgac tgacgataac
     1141 gcgttctgcc ccattgataa tgaacgtgcc tgtatcagta atcagaggga agtcacccat
     1201 gaatacttcc tggtccttaa tttcacctgt ttcgtggttg acaagacgga tctggactcg
     1261 gagcggtgcg gcatacgtcg tatcgcattc cttacattca cgaatgtcgt acttcggttc
     1321 ccctaattta aaaccctcaa aggataaaga caggttgccc gatgcatctt cgataggaga
     1381 aatgtctttg aagatatctt taagtccctt ttcgaggaac cattcgtagg atttattctg
     1441 taaatccaac aaatgaggca ttttcaagac ctcgttaatc ttagcatatg tgtatcgagt
     1501 ccttttccct acttgtacag gtttgaacat gctgattctt caccccttat aaaaagtaaa
     1561 ccattataat cgcgccaagc gcgtcgttaa gcgcttcata aagcgacaaa aaagcaaggc
     1621 tcttagagac attgaacctt gcttaatttt ttgctcaccg gaagcgtttc ctccatgcat
     1681 ctcataacat caattgaaca ttctgcaatg agttattata gcatacagaa aatcttccgt
     1741 caatattaaa ttttaatgaa ttcagaaaaa cggtttacgg ctgaacgcgt gccaaggtgt
     1801 acttcttctt gcctttgcgg acgatgacaa atttcccgtt tgaattctta tggggtttta
     1861 cgatttcatc gaggctggta actttgacgc cgttaatggt gacagctccg ttattaatgt
     1921 cttcacgggc ctggcgtttc gacttataga tgttggcatc gatgagccag ttgacaacgt
     1981 ccttgtcttc gttggtaacg tcgacggtcg gcatcttgcc aaaggcctgt tcgagttcat
     2041 gttccgaaag atcggcgatg ctgccatgat agagggcttc cgtaatattc tgggcttcct
     2101 tcaaggcttc cgggctgtga acgaaggtcg tgacttcttc cgccagacgg cgctgagcca
     2161 agcgtttttc cggttctttt tcaacggctt cggccaaggc gtcgatttct tctttgctga
     2221 ggaaggtaaa gtatttcaaa tatttgatga cgtcggcgtc gtcctggttg agccagaact
     2281 gatagaattc gaaaggcgac gtcttttccg ggtcgagcca aacggcaccg ccggcagtct
     2341 tgccgaattt cgttccgtcc gatttcagca tgaggggaat ggtcaggccg aaggctttgg
     2401 cttcgctgcc gtgcatcttt cggatcgtgt cgatgccggc cgtaatgttg ccccactgat
     2461 cggcaccgcc gatttggagc tgaacgtcat tgtttgaaaa gaggtgttca aagtcgatgg
     2521 actggagaat ctgataggaa aattcggtga aggaaatccc cnnnnnnnnn ncgacttcct
     2581 tgctgagcat gatgttgacg ctgaacagtt tgccgtagtc gcgaaggaat tcaaggaggc
     2641 cgattttgct gagccagtca tagttattca caaaggtgat attctctgaa tcaccgaaga
     2701 ggtgcgtaaa ctgggctttt aatttttctg cattggcctt gatggtatca atagtctgca
     2761 gctgtcgttc cgtcttacga ccgctgggat caccgatggt acccgtaccg ccgccgatga
     2821 cgacgaaggg atgatgaccg gcattggcaa agcgcttgag catcataaag ggaatcaaat
     2881 gaccgatatg catgctgtcg cccgtcgggt caacgccgca gtaaagagag atgctctttt
     2941 cttccgtcag tttccgcagc ccttcttcat ctgtcatctg attgacggcg ccgcgccatg
     3001 acaattcatc aatgatattc atcgaattcg ctcctt
//
LOCUS       DAWDXZ010000179         2990 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_6980_length_2990_cov_9.381601, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000179 DAWDXZ010000000
VERSION     DAWDXZ010000179.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6980_length_2990_cov_9.381601"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    337..346
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          841 a          845 c          676 g          618 t
ORIGIN      
        1 ataaagccga ttgtggtata ataagaagct agtaatattt caatttatat ataataacct
       61 tataatgagt acaaacgtca tgtaggagga aaaaatgtat acacccagac gcagagcaaa
      121 aaaagccaaa gaggccggtc cattccgccg catccgcctt tttatcgcct tatgccttgt
      181 cgtttttgcc ggcttagggt ttggctatat ttttgctgct tatcaaagct tgccgacggt
      241 aagcaatagc atgcggcctg ccgtatcgtc ccaggtcttc gatgcccacg ggcggctgat
      301 cacaaccctt cattccgatc agaaccggct tcctatnnnn nnnnnngccg aagacaaccg
      361 tttttatcag cacatcggca tcgatcccat cggcatcgtc cgcgccttgt tcaccaacct
      421 gaccaaccgc ggtatcgccc agggcggcag tacgattacg cagcagctcg caaaaaatgc
      481 cttcctgtct caggaacaga cgatgaagcg aaagattcag gaagccatgc tggccttaga
      541 aatcgaacag aagtacagta aaaaagaaat cctcgaaatg tacatgaacc agatttactt
      601 cggccaaggc gcctacggaa tccagacggc tgccaagaca tactttaata aagacgtcag
      661 tgaattgacc ctgacccagt gtgccctctt ggccggcctt cccaagagcc cgaactacta
      721 ctctcctttc aataacctga atgaagctaa acaacggacc aacgtcgtcc tcgaccagat
      781 ggtcaagtat ggctacatct ccgcctcgga agccgaacag gccaagaacc aggatctcga
      841 cctttcgaag aaccaccagg ctcaagaaag cgatgaaaat gcatccttca tcgattacgt
      901 atcccagcaa gttgctcaaa aatacggtga cgacgccctc tacaaagaag gtttgaagat
      961 ttacacgact ctcgacgtcg acaagcagca tgccgctgtc caggcccttc acaacctgcc
     1021 ggacaactat accgatgaaa atgggctgac tcagccgcaa ggcgccattg tctccatcga
     1081 tcccaagacc ggctacatcc tggctatggt cggcggccgc ggtcaagaca gcttcaaccg
     1141 cgccagccaa gccgtccgcc agccgggttc cgcctttaag cccttcgtct atctcacagc
     1201 cctgcagcat gatatgacgc ccgatacgac gatggaagat aaacccgtta cctacggcag
     1261 ttggagtccg agaaatgcca gcggctccta ccgcggctcc atgccgttaa gcgtcgcctt
     1321 agcaaactcg gtcaatacga ttgccgtcca gctcgccgat cgcgtcggcc ccagcaacat
     1381 cattgccaac gccaaaaaaa tgggcatcac caccctcgac aataaggatg acaacctggc
     1441 catggctctc ggcggtctga cccgaggcgt cacgcccctt gaaatggcca gtgcttacgg
     1501 cacctttgcc aataagggcg ttcacgtcaa accgacggcc atcatcaaaa ttctcgaccg
     1561 caacggcaac gtccttgaag atgaatcaac cctcgaaaaa gaggaaaaca agactcgcgt
     1621 catgtccgaa aaagaagcct atgaaatgac cgtcatgtta gaaggcgtca tcgaatacgg
     1681 caccggtacc gccgcatcgc tcggacgccc ggccgccggc aagaccggta cgacagacga
     1741 caataaagac gcctggttcg tcggctatac accggatatc gtaacggccg tttggatcgg
     1801 tgacgatacg ggttcgcaca gccttggcga agtctacggc ggcaccattc cgactgaaat
     1861 ctggaaagac tacatgtcat ctgctgcatc agacgaacaa gaccgggaat tttccgttcc
     1921 ggcaggtatg gaacggattc gcgcccgggc agccctctcc aataacagcg cccgcgtcgc
     1981 agacgacgca aaaaaagcgt cggatacaga aaagaaagac tccgacgata aagaaaagac
     2041 cgacgaaaaa gcagagtccc gaagtgaaga aacatcgcga cagccgtcgg aagaagcacg
     2101 aagacagcag gaagacagta acaacaaagc acagtaatgc caaaagagca gccgcaccgc
     2161 aaacggtgca gctgctcttt ttctgttgtc cggaccttac tttttttcat atataaacaa
     2221 gagacaggtc catcttctct gtaacctccc ccgaaccggc tgatatccca tttttcgtag
     2281 aacctgatag gggggcccgc ttttattaca cgtatatagg ctcttgtcct tccacacaga
     2341 ctgaatctac ggctgccatt agcttcgccg tcagtccccg ccagcggtga ccaggatgta
     2401 tcggcccgcg tcgacgctac tcccatcata ccgatagcgg atgactccga cgataactgc
     2461 ctcatcagag acgagcttca gtatcgtccc attgacaaaa tcatacctga agtcgtcttc
     2521 catttccgga agcgccggca catcgaattg gccaatcaag gcagcctccc tcgactatat
     2581 ttattatccc caaaaaaggg ggcgtcattc atccaaaggc cccttaaaac agaaaaaagc
     2641 agccccaaac ggagctgctt ttaagtcaca caaacctttt tatgagatta tggagagatg
     2701 ttagtcgata cagtaacggg agaaaatatc gtcgaacgtg tatcctttat tcataatgaa
     2761 agcctccttc aaaagacttc aataacaatt atcagttgtt gattataagt ttacacgttt
     2821 ctctcccctg cgtcaaccga attgccagat taattcggca acctaactat tcgctaaagg
     2881 gattcatact cagactttga caaccttatg gatgatcgtg ctgagaatca tgatgatgac
     2941 gacgacggga acggtaatac cgaccggcgt gtcgacgctg agcaggtaac
//
LOCUS       DAWDXZ010000180         2971 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7019_length_2971_cov_8.140947, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000180 DAWDXZ010000000
VERSION     DAWDXZ010000180.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7019_length_2971_cov_8.140947"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1895..1904
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          574 a          856 c          841 g          690 t
ORIGIN      
        1 ctgcggccca cgtcgaggcg caccgtccag aagtgaggcg ttccgccctt atcccggcca
       61 agaagggtaa agtctgccgt gccccgctga tgatcgtacc gggactgacc gttcaggaag
      121 cggccgaatt ccgtacggtt ctggccgtag tcggcagaca ggcgcaggtt ctgtccgatc
      181 tgccagcggg ccccgatgcc gacgacgtcg gcaaaacggt caaagatatc gacatcattg
      241 ccgtggttat tttccgagaa ccacggctgt ttgtcctgcc tgaaagagtg gaagtaccaa
      301 gcctgaaggc cgaaatcagc gccgaattgt ttcttgacct ggacgaaagc cgcccgatcg
      361 agaggggcaa tgacgtcgcg ccggaggatc gtcccttccg taaggatcgg ccgaccgatg
      421 gctttttcta aagccgcccg gggctgcttg ctggccgaat cggcactgcg gacgagatct
      481 tccagggctt tcaggtattc cgacagcagt ctgggaatct tcagcccgta attcgggtcg
      541 tttccattga agtcggcact gccgtaattt tccttgtaca attcatcgat gagatggtcc
      601 agcgtcgagt cgaaccggta ctgtgaatac caacccgctc cgtcttggcc ggaatctttc
      661 caatcgaggc tgccgatgtc ttcgatgccg tcatagacat aatcgccggc cctatcgctg
      721 cggctcacat tacgccacat gccgcttccg tccaagggac ggtaggtcgt ccccatttca
      781 tcgtccttgc cgtaatagcc ccaagcttct ttggccgtat gctcggcaat ggcttcatag
      841 ccccgatgca gttccgcttc ccgggaagtg aggaaggccc ggccgtcacc gagagccctg
      901 tcgtcactga ggctgatgga aaagtattta ttcatttcct gtttataggc cttttcttcc
      961 ggcgagtcga agtcgctgat gtagctgtta tagtagacat cggccgcctt atagacgacg
     1021 gctcccgtgt ctttatgctt caagcggtac aataccttgc cgtgaatcgg cacgttgagg
     1081 gtaatgccgg cttgagccgc ttctttgccg taggccgtgt tcacgatgtg ctggaggcgg
     1141 cggattacct cgccctgctc cttcgtgtcg gccccgtttt ttacggcatc ccgcagctgc
     1201 tggtagaagt agaccttgtc gtttttcccc ttataggact tattgtaaat atcgttgggg
     1261 ctgccttcgt cgtagaccgt tccttcgcgg tcgggatcgg tcgttcccgg cttcaagggg
     1321 ttggcgccgg ccatcttggc cagatcataa ggccaatcgt cgcgattgag accgatcaat
     1381 tccgataccg tcggcggccg atagaagacg gcgtgcgtcg aatgggtata agccgaatcg
     1441 gagatacccg tactgtgctt aaaggacccg tatcccaggc ggacctgggt atcctttccg
     1501 gtccaaacgg cgcggacgcc gtcgaattct ttcccgaacc aataaccggt gacccccatg
     1561 ggttccgtca ggcggccgat ggagaagtcc catttccgta cgcggtgcgt caagtccgct
     1621 tcttcgacgc gctgatgctg aaggccttct gacaagggca cggtctctgc cgtatcgacg
     1681 gctgccgtcc cggaggcgct gcccaagagg cgcagattgg tatcgtctcc caagcgggca
     1741 tcgacgccca aacgcaggcg atggctaaag ccgtgttctc cccggtcata catgttgcgg
     1801 tctgccgtat cgatgtacgt atctgcccta aagggcggca gctggcggtc ctgtccctta
     1861 tgggaatccc accgcaggcg gtagtcaccg atgannnnnn nnnngatgtc ccagccttct
     1921 cccttagact cgtcaaaggg gcgggcgatg aagtcgttca tgcgaaccgg cgttctggca
     1981 gcccctgcgc cttcagcagc ggaagccttc ttcccttcga ggatgtcccg ggcatcgcgg
     2041 cccagtttaa agttgacccc aaagcggtag acccgctgct gcgtcgcccg gtcgtcgtcg
     2101 cggtggttga acaggttgtt gatgccgaag tagaccaagg aatctttatt gatctttttc
     2161 tgcaccatga ggttccaaat accgaaggtc ttcttctggt accggggctt gcggtacacg
     2221 gccccttctc cctgaaggat atcgggccag tagttcgacg aatcggaaag ggaattgctg
     2281 tccagcatgt ggatgtagta attgccccat aaggacccgc gccagccgct tgccgggtcg
     2341 tcatagtcaa ggccgatatc gaccttatgg accggcttgt cgaggaggcg gcgcggcata
     2401 tcagagtcgc tcttattgat ggcatggagc caggtatacc ccagtttagc cgtccaatga
     2461 cggcccagtt tctgcttcac ttccgcttcc aatcccgtaa tttcagcgcg gccgatattc
     2521 ttaaagctgt aaatcaaatc gggggcccgc atccatttgg ccgaaaagcc caaatcaggg
     2581 gcaaaatcca tgtagcggcc ggtaaaatag acggtcatgt aatctttgat gcggttgtgg
     2641 aagaagccca atcgggccga cgtattatca ttttcccctt cgatgccgat gtcgaagtta
     2701 accgacgttt ccggcttcaa atcgggattg ccgacccagt accagcccat gcgggccacg
     2761 ccgatgccga cgggactgct gccgtacatt tcccagttat accagagttc gcccatgccc
     2821 ggttcggtat agcccgtgcc gatattggct ttaaagcgcc ggtgttcgtt gccgccgaca
     2881 ttgtgggtca gccccaaatt tcccgaaaga tggctgccaa agaggctgct gtcgtccagc
     2941 cggagaatag gggtcacgat ggtcctgtcg t
//
LOCUS       DAWDXZ010000181         2968 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7028_length_2968_cov_7.665294, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000181 DAWDXZ010000000
VERSION     DAWDXZ010000181.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7028_length_2968_cov_7.665294"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          737 a          850 c          770 g          611 t
ORIGIN      
        1 cgcacatctt agtccgcaaa atcctgcgtg cgcccttgca gcgcaccgtc gaagcggccc
       61 atacgcctga cgaagcgcag gtcgtccgcg aactgcaaaa cgtattcaag ttagacatta
      121 aagagtaagg aggcctttcc atgatttcct tagaaaaatc aagggccgcg ttttccgaag
      181 cgcagaccct catgccgggc ggcgtcaaca gcccggtccg ttcctacagc cgtatcggct
      241 gcccgccgcc ttttatcgcc cgggccaagg gcagtaccat catcgacatc gacggcaacg
      301 agtatatcga ctacgtcgga tcctggggtc ccatgatcgt cggccacgcc catccccgcg
      361 tcgtcaaagc cctccaggaa gcggccgaac ggggcacgag ctacggcgca ccgaccctcg
      421 tcgagtccga tttggccaaa ctcgttcgcc aggtctaccc gtccatcgaa aaactgcgca
      481 tggtcaattc cggtacggaa gcgacgatga gcgccatccg cgccgcccga ggctatacgg
      541 gccgggaaaa gatcgtcaaa ttcatcggct gctatcacgg ccacggcgac agcctcttag
      601 tcaaggccgg cagcggcgcc gccaccttcg gtcagcctga cagcccgggg gttacggccg
      661 gcacggccaa agataccctg accgtccctt ataacgatgc cgccgccttt aaggccctga
      721 tggaccgtag cggcgacgat atcgctgccg tcatcgtcga acccattgcc ggcaacatgg
      781 gcctcgtcct cccccaagaa ggctacctcg acctcctccg ggaagaaacg aagaaacacg
      841 gcacggtcct catctttgac gaagtcatgt gcggtttccg ctcggccctt cacggggctc
      901 agagcctcta ccatattgag cccgacatga cgaccttagg gaagatcatt ggcggcggcc
      961 tgcccgtcgc agcctacggc ggaaaaaaag ccatcatgga ctgcgtctcg ccggagggtc
     1021 cggtctacca ggcaggcacc ttaagcggca accccctggc catggcagcc ggccttgaaa
     1081 cgctgcgcat cattacggaa gatccgaact atatcgaaaa ggtcacggaa aagacatcga
     1141 cccttaccga aggaatccgt caggccgccg aaaaagccgg cgtccaggtc caggtccccc
     1201 aggccggcac catgttcagc gtctttttca gcgaccgacc ggtcacgaac tacgccgatg
     1261 ccgaaggctg cgacgccgag gccttcaaga gctacttcct gtctatgctg aatgacggca
     1321 tttacctggc tccgtcgccc tttgaaacga acttcgtctc cggcgctcac agcgatgccg
     1381 acatcgaaaa gacgattaaa gctgctgaaa aggccttccg caccttataa tatatataag
     1441 accgaaagaa ggtacatcgt gataaatcag cccgacaaaa aggaaaagac aaaagcaata
     1501 caattcagaa tcgatgaaac gttacttaag cgatttaatg aagtgacaga acgctatagc
     1561 gttaataaga gtgcattaat tagaagctgg atagaacaat acctggaaaa gcataaagat
     1621 gattaaataa caaacttatc cccacacaaa aaatcccgtc tgagccatcg ctcaaacggg
     1681 attttttcac agcccttctc ttttaggaaa agctgtctct tataagattt tcgacaagaa
     1741 agcctgggtc cgttcttcct tcggatgaga gaagagatcg tccggtttcc cctgttccag
     1801 aatcttgcct tcatcgacga ataagactcg atcggccact tcgcgggcaa aacccatttc
     1861 gtgggtgacg atggccatgg acatgccgtc gtgggccagg tctttgatga cgtcgagaac
     1921 ttcgcggacc atttccgggt caagggccga cgtcggttcg tcgaagagga tgaatttcgg
     1981 cttcatagcc aaggcgcggg caatggccac acgctgctgc tggccgccgg ataaggtttc
     2041 gggataggca tccttcttat cggccagacc gactttttcg aggagctgaa gagccacttc
     2101 cgtcgcggct tccttgcttt ccccacgggt cttcatgggc gacaagatga tgttttccaa
     2161 gaccgtcatg tgcgggaaga gattgaaccg ctggaagacc atgccgactt ccttgcggac
     2221 gctgttgatg ctcttagcat cgttcatgct gatgccgtcg acgatgattt cgccggccgt
     2281 cggttcttcc aggtaattga tacagcggag aatcgtactc ttgccggacc cggacgggcc
     2341 gatgatgacg acgacttctt tatcttgaat ggtcaaatcg atgccccgaa gaacctcgtg
     2401 tttgccaaag ctttttttca accctttaat ttctatcatc cgttgcaaac ctccgttcca
     2461 aatagcttac caactgggcg atggtaaagg tcatgaccaa ataaataaaa gcgacggtca
     2521 gccagatttc aaacgagccg taggtcttag cgatgatcag ctggccccgg cgggtcaatt
     2581 cttcaaagcc gatgaccgaa accagggacg tttctttggt catggaaata aattcattgc
     2641 caagaggcgg aatgacgtgt ttgaaagcct ggggcaaaat gatgtagcgc atagtctgca
     2701 tccacgacag gccgagagac cgaccggctt ccatttgtcc cttatcgaca gactgaatgc
     2761 cggcacggaa gatttctgcc acataagccc cactgttgac agaacaggcg gcaacggcag
     2821 cgacgaaggg gttgatccgt tcgccgatga tcatgggcag ggcaaagtaa atcaaaaaga
     2881 tctggactaa gagcggcgtc ccgcggataa tattgacgta gcagacagcc aggatgttca
     2941 atattttgac tcctgacaag cggcacac
//
LOCUS       DAWDXZ010000182         2965 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7033_length_2965_cov_7.238144, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000182 DAWDXZ010000000
VERSION     DAWDXZ010000182.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7033_length_2965_cov_7.238144"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1632..1641
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          802 a          686 c          780 g          687 t
ORIGIN      
        1 caccagcgtc gatgccatct gtgagcagat gggccggaca cctccctggg ccgaagggct
       61 attgctccgg gctgatggct acgaatgtga tttttataaa aaagattgat ttcccggtac
      121 ttaacatagt gattcctgtc ctttcactat cagagggaat ttcctcggat atttatttta
      181 agaaaggcgg gattcgctat gaagtttttg attcaagaag atgaatttgg cgtgtttgct
      241 gatcagagag gtgtaccaag ggtcgacagc ctgtttgttg cagcgacttt tgaaaaacag
      301 cattataacg ttctgcgtga tatcggacga atcactgcat ccaattctgg attaagccca
      361 gaattcattg cactcaattt tgagggcaat acatatcgtg atgccagagg aagaaaactg
      421 ccgcgatacc tgctgacccg cgatgggttc accatgctgg ttatgggcta cacaggctcg
      481 aaggcgatgc acttcaagga actctatatc catcgtttca acgagatgga gcagtgcatt
      541 cggtcgctcc tgtctgcccg gcaggaattc ccgatgctga cggacatgat ctgccgcctg
      601 catgaaagcc cgaagccgta ccacttcagc aacgaatgtg acatgttgaa ccgcatcgtg
      661 ctgggaaggt ctgccaagca gttccggctg gccaacggca tcgaaaaagg gcagagcatc
      721 cggccttatc tgactgcaca gcagatccat gctctggatc ggctgcagca cctggattac
      781 ggcctgctgt actcctgccc ggatttccag cagcgcaagc agctgcttat ggcttattac
      841 aagacggaac tggagggatg aaacatgttt tatgtaaaag aaccacttaa ggatggtgtc
      901 gatgtgacgg tcgaaatcaa cgatgagaat gtattctgcc gctgcccggt ctgcagcagg
      961 gaagtacctg tcaacttgca ggacgtcctg tcagacagtg acgctgacct ggtgggaacg
     1021 gcagtattgt gcgatgaatg tgctaaggga tggatgaaaa atcatggaaa gaaatccgaa
     1081 acgtaatgcc gagcattacc ccgacccgac ggcgtatcag gcaatcagga acgtggaacc
     1141 ccaaaggttc ccgttcatgc ctgttgtgta cgtgtgttcg ccctatgccg gggatgtgaa
     1201 agaaaatatc cggaaagtct gtgcctactg ccgctatacg gtagaccagg gatgtatccc
     1261 tctggccccg catctgtatc tgccgcagtt cctcgatgaa gaatcggaac gggaactggc
     1321 actctttatg gatatcgcac tattgtcccg ctgcgtcgaa ctctgggtct ttggcgatgt
     1381 gatttcggcg ggaatggaaa aagaaatcca gtacgcccaa agaaaaggaa aaccaattcg
     1441 gtatattaac gaggtgaaat aagatggatt ttacactgta taggtcagac tttgctggcg
     1501 tggaagcgaa ttgccgctat cccaggcagc agaaaatcag ctgtgccgag gatctgaagg
     1561 cggcagccgc cttcgatcat gtctgcgtag ccttcaaaga ttgttatcgg aaacgggaga
     1621 atttcctctc tnnnnnnnnn ntctgccgac gtcctggtca tggattgtga caatacccat
     1681 tcggagaatc ctgctaactg gatgaacccg gaaaagctcc tgaacatgct gccgaaagtc
     1741 tcagtggccg tcgtgccgtc acggaatcac atgaagccca aggaggggaa gtgtgccaga
     1801 ccgcgcttcc atgtttattt cggaattccg gatatcacgg atgaacagca ctatacagaa
     1861 ttgaaacatg caatctaccg tgcctatccc ttcttcgatg aagcagccct cgatgcagcc
     1921 cggttcatct atggctgtcc cgtcgagaaa gtgttgtggc aggacgggga aacgacaatc
     1981 gaccaggtac tcaacgtggg agataccgaa tcgcgcagta tcccagctgg atgcaggaac
     2041 agcaccatga gccgctttgc cggccgcgtc atcaaacgat acggagcgac agaaaaggcg
     2101 taccagattt tcctcgacga agcgggaaag tgcgacccgc cgctccctga tgaagaactc
     2161 aatcatatct ggcaaagtgc tgtacgcttt ggtgaaaaga ttgccaagca ggtagggtat
     2221 atcaatccgg agcaatataa caatgatttt ggaaccaagg aaagtttaaa gccggaggac
     2281 tacactgaca tcggccaagc gaaagtacta gtgcgggaat atggcaatga actgaaatat
     2341 acaccagcaa ccgggtatct ccgctatgac gggaagcgct ggattgaatc caaatctcaa
     2401 gccatcggtg ccgcagaaga atttttggat ttgcagctta atgatgcaaa agacaataaa
     2461 gataaaacca tgaaagctct attggaaact ggaattaatg aagccgccat tcgtaaaggc
     2521 ggcagcgctt tggaaaaact tattgataac agtgtgagac ctatttatta cgagtatctt
     2581 ggtgctgtca gctatgagaa atttgtctta aagcgccggg atataaagta tatcctgacc
     2641 gcactgacgg cagcaatgcc catgattgaa ataccgtatg attctttgga cagtgatcca
     2701 tttctcttaa actgccctga tggtacctat gacctgcggc aaggaatcag aaaccggcat
     2761 gaacatagag cggaggattt gcttaccaaa atgactgcgg ctgcgcccgg tgacgaaggt
     2821 aaaaagctct ggctggattc gctcaaccag atattccagg gtaaccagga actcattgat
     2881 tatgtccaga tgattacagg tctggctgcc attggccagg tagaactgga ggcgatgatt
     2941 atttcctatg gtgaaggggc caacg
//
LOCUS       DAWDXZ010000183         2961 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7043_length_2961_cov_6.767034, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000183 DAWDXZ010000000
VERSION     DAWDXZ010000183.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7043_length_2961_cov_6.767034"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2078..2087
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          774 a          718 c          755 g          704 t
ORIGIN      
        1 aagtgacgtc gacgtaatcc cgtcgacgtc atagtcacca tagatgacga tgcgctcttt
       61 atcttctacg gcctgcttca gccgacggac ggctgtttcc atcccgtcca tcaagaacgg
      121 atcggccatg tcgttcaatg tgtcctggag aaagtgacgc ccttcttcga catcggcaat
      181 gccgcgatta gccagtattt gggccaaaat cggcgatata tgcagttgtt cggttaaggc
      241 ggacgtaagg ccctcttcga agggggcaaa ctgccatcgt ttctccattt gcatcacaac
      301 tcattctcag gaataatcac tatcaataaa tcaattatta ttttatcact ttctattctc
      361 aaaataaaga ctaaattaat atgcacttag caagattctc atcttagaaa tggattttac
      421 catttcttta cagtctccac ttcgcctccc ttttcgagga tagcgaaaaa ggactattca
      481 tgaaaaatca cgaatagccc tctgtatcat gtcgatatcg aaaacatcga gtttacgacg
      541 acaacgcttc cgaagtcgtc ccggccttcg ccgtttccct ttccttctct gtgggattga
      601 tatcgtcttg agaaatggtc aggcgatgac gggccgcttc taagatgagg tcggccgtct
      661 tgacgatgcc gtagtcgctg gatttgaggc tcaaatcgta gttggcccca taaccccaat
      721 aggaatcggt gcagtacata cagtgatccc gccgttcacg attgacgtgg ttgcggagtt
      781 tacgcgcccg gtcggcgtcg atgtgttccc ggagcatgat gcgctggacg gcccaatcag
      841 gatcggacga aatgaagaca ttgaaggtgt tcggtcgttt gcgcaggacg tagtttgcca
      901 aacggccgac gatgacgcag gaatgccctg tcgtcagggc ttcaatgact tcagcttcat
      961 cgcggaacat ttccatctga ttcgattcat atcccgacgc atagtggacg taatcttcgt
     1021 ataccttaga caggagcggc cccaagcggc gcttctggtc cttgcaccag gcgatgacag
     1081 tttgcggatg ttctttctgg gtccggttga aaatttccgt atcgtagtac ggaacgccta
     1141 atttacgggc cacgagtttc ccgatattcc ggccgccgct gccgaattca cgcgaaatgg
     1201 taatgactaa ggggctgtcc gtatcttcct tcatccaagc cggatggttg gtacggccgg
     1261 cgatttcgcc gatagcccga tgagagaaaa tcagacgttc aatgggccct gccatggtac
     1321 agcctaaaag gatcagtacc aggccgatga cggcgttggc cgtaattggt tccgataaga
     1381 taatcgaggc caagatgagg gcgatgatag gcttaataaa aaaagcaaag gacgcattgg
     1441 acggacccgt cagttcaatg gccttcatga agaacaggta gccaaagccg gtgacgacca
     1501 aggagcagta caggagcggc caaatggtgc gggaattgat accggcaatg atcggatcgc
     1561 cgtggaaaat cagaatgacc aagaggaaga ggcagccgat gagaaaactg aaggcgtttt
     1621 cgacgttgcc ccccaatttc ccgatccgca gtttccccaa ggtcgtgtaa aaggcaaagg
     1681 aaatagccgc tgccaaggac accagaagtc ctacatttcc gttctggatg atatccacgg
     1741 gattggcgac gatgatcagg ccgatgagac tcaataccaa ggtgatggcc ttcttgcgcg
     1801 taaaggcttc gtggatgaga aaataggaaa aaatcatgac gaagatagga ttgctggaaa
     1861 agacgatggc cgccaggctg gcattggcca tgttgacgcc gatctggaag aggatcatgc
     1921 tgaagcagat attgaccagt cccaagacag ccaaataccc ccaatcagtc cgtctgagac
     1981 gaatctgatg tttcttcata tcgcgaacgg ccagcggcat gagcaggatg ccgcctacga
     2041 gaaagcggag aaaggtcagc tgaaaaggtg taaaggcnnn nnnnnnnaag ggctgccagg
     2101 acaatacaaa gcgtacccat tttagatgac atgaaagagg ccccctagtt catctaatta
     2161 ttatgataga gaataaaaaa actaataatt caatcacgat tcattataca ctacttcccc
     2221 ataaataaca aacaacaaaa aggccctgca tggtggaaac caccgtgcag agcctttccg
     2281 ctatttatct atgcagcaag tgcatttaaa ttagtcttca aaacccttct ggagacggac
     2341 gtaggaatga cgacgattct tagcgtcagc ttcagtctta gcatagaggg cttcggcttc
     2401 ttccgggaag ctcttcttga gggatgcgta acgaacttca ccctgcaaga attcctggaa
     2461 tttgccataa tccggttctt tggaatcgag gctgaacggg ttcttgccct gttctttgag
     2521 ttccggattg taacggaaga gatcccagta accgcattcg acagcgagtt tttcttctgt
     2581 ctggctcttg cccatgccct tacggatacc atggttgatg caaggagcgt agcagataac
     2641 caaggacgga ccatggtaag cttcagcttc ggtgatagct ttgaggagct ggttcttgtc
     2701 agcgcccata gctacctgag ctacgtaaac atagccgtag gaaatagcca tcatgccgag
     2761 gtccttcttc ttggtgcgtt tgccggcagc agcgaactgt gcgatagctg cagtcggggt
     2821 agctttagag gactgaccgc cggtgttgga gtaaacttcc gtatcgagaa cgaggacgtt
     2881 gatgtcgttg tccgaagcga ggacatggtc aacaccgccg taaccgatat cgtaggacca
     2941 accgtcacca ccgatgatcc a
//
LOCUS       DAWDXZ010000184         2956 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7049_length_2956_cov_5.105481, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000184 DAWDXZ010000000
VERSION     DAWDXZ010000184.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7049_length_2956_cov_5.105481"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1852..1861
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          768 a          621 c          807 g          750 t
ORIGIN      
        1 gtgaagcctc ccttggcggg attaccttga gaagtcccat gagttacgtt cgcagcattt
       61 acggatctcc tgcggagcgt tatatgacca agaaccgatt taataccaag gctgttgcca
      121 accgctatgg aaaggggttt tacgtcattg aatatgctga taaggcagcc gtagaggaac
      181 tttatgtaaa cgcccacaac ggcattgcga cgcctatggg cgttgccgtg ggcgttccca
      241 aatcgaccgt cgacagactg tatggcagta gtacgtattc gcagggcaag tactattatt
      301 atacccggaa tgccatagcc attatgatcg tttacggaaa cgacaccaat ggggtcacgg
      361 tggttaaatc cattaatata cagtaccagt cgtaacaggc tcaatcagga ctgcctatgt
      421 cgttaccatg ggcagttttt ttgcgtcgca tgggggcgat aaaacccggt cggagaattg
      481 tgtttataat taaacggcag gcgctgtaaa catattgata gaattatttt tttatgatac
      541 gattcagtta tattgagaag caaaccgata atagaggagg aattttctat catgttcaaa
      601 aaagtggcag ttctggcatt ttcccttact accctgctgg cgagtacctg cctggccgtt
      661 atgacaggtc ccgttccgga cagcgaggcg tcgctcggcg gcatcacctt aggaagcccc
      721 atgagctatg tgcgcagtat ttacggcgct ccgacggatc gagtcccgac taaggactat
      781 atccggcaag atgctttcag caacatctac ggcaacagct tttacgtcat cgaatatccc
      841 aagaacagca gtgtagaaga actctatgtg acggccaaca acggcattgc cactcccctg
      901 gggattaccg tcggcattcc caagtcgacc gtcgataagc tgtatggtga aggcagcttt
      961 gtccagggat cctatcgcta tctgacgcag gaaggcaaag tcattcaaat caaatacgta
     1021 gacgacgcca atgatgtcgc cgtcgttaaa tccatctata ttcgctattc ggcgtaataa
     1081 atcaaacaaa ggatgataga ggaggatttt gtattatgtt taagaaaata gcccttatga
     1141 tctttaccct ttctatgctg gccgtcggca gttgtttcgc cgctatggtc ggcaacgttc
     1201 ctgacagtga agcttctctg ggcggaatta ccttgggcaa taagatgact tatgtccgca
     1261 gtatttatgg ggaaccgacg gaacagagac gggcgaagac ggtattgaat gaaaatgcaa
     1321 tcttgtatcg ctacggcaag ggatttttta tcctcgagag gctctatgac aataccgtcg
     1381 cagaactgat gaccagtgct aataatggga ttgcaacgcc catggggatt acggttggcg
     1441 ttcctaagtc tacggtggat gcattgtata gcggagggcg gtccggaaac ggatatacta
     1501 gctatatggc gcagaatggg gccttgatta aaatcacgta tggtcgtgat gctaatggcg
     1561 ttctggtcgt tacgtccatc acgatgaggt tccctgagta agctgttcgt agaatacgta
     1621 ctgcctgtgt ctgagacatg ggcagttttt ttatgcctca aaaacgtttt ttataggcgc
     1681 ataaataaga ccgccgggct gtcgagggat ggcggacagt ctgacggtct tgccgtattt
     1741 tgaaggattt atagtggtta cttgccgaat aaacgagcgg gcaatggaat ttgggccaag
     1801 atgacggcta cacagacgtg gacgcagccg acaatttctt gtccggtcat gnnnnnnnnn
     1861 ngtgggcttt ttttaagaga aaatgatcga tgaccaagat gtgaagggcg aagaagatgg
     1921 cgcagaagaa gaccaggagg tcaccgaagg caatttcaaa atcgccgttg atgcagagaa
     1981 aatagaatcc ggtcatggcc aggatgacgc agaagacgat gattcgcgga accttgcggc
     2041 cgatgaaaaa tcccatgagg gggaccagga cgatgtagat ggcggtaatg aatccggctt
     2101 ttccggccgt cgtcatggtg atgccgacct gctgcagggt atcggcgatg cctaaggtga
     2161 ggccgcacca ggcgccgccg gtgagggtaa tggcatcggg cttgagctgc tgccatatgg
     2221 gcggatacgt cccgtcatct cttttatcgg taaataagag gatgaccggc atgaggacga
     2281 tgatggcgat gacgttacgg gcggcgctga aggtgaaggg accgatatag tccatgccga
     2341 tgctttgggc gacaaaggcc gatccccaga tgagggctgt gattaggaga aaaatcgtgc
     2401 ccttggcttc tgtttggtac attttgataa atcctttcat aaaatatgga aaaaacaata
     2461 gtactataat ataataatat tagcaaaaag taaataaaaa aaacttgact tcgtgttgac
     2521 tccaaggtgt atcctcgaga tacgttaaag gagcgacggc aagactgttt tgaaacagta
     2581 gatttggcag ccgcacagat acagtgttac atggtctttg tatggccata cagtttttta
     2641 gaagggtagt gagtgtgatg agactgacga gaaaacgaat gagtttggct gtactcggat
     2701 tggcggtcgg tgttttttta ttctttttaa cggggtatat gaactgcctg caggcaagtc
     2761 ttcaaggggc ttcccataat agttctttta tttttggtga tgccgggcct tatgcggcta
     2821 agacgcggct ttcttttgaa ggcggtcatg ccgtcctggg gttatacgat acgccggcgg
     2881 ccttggaatt ggccgaacgg cagccgctgg tcgtaccgct gcagcgaaaa gacggacgaa
     2941 tcgtcctgcc tgatat
//
LOCUS       DAWDXZ010000185         2926 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7107_length_2926_cov_8.138628, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000185 DAWDXZ010000000
VERSION     DAWDXZ010000185.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7107_length_2926_cov_8.138628"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          661 a          739 c          786 g          740 t
ORIGIN      
        1 gccttctaat atcgtagtcg accatgaagg agacatcttg tttcagtccc cttacgggga
       61 ttttggtacg ccggcgtccg cttcctgagg tcaggaggaa ccaaactgga cagtttcagt
      121 ccccttacgg ggattttggt acgccggcac aaacatgtcg aaatttatat acatggcatg
      181 gccggagttt cagtcccctt acggggattt tggtacgccg gccgaagaca tggaacatga
      241 tatggaactc atcgaagagt tggtttcagt ccccttacgg ggattttggt acgccggctc
      301 tgtcttccag tacccgctcc cagactgggc tcacaggcga ttttttcaaa cctcgggctt
      361 ttttcgcctt ttcggctgac ccgggtgaaa aataaaaatc aggcaatggc gattatcggc
      421 ttggttatgc cgtttttcgc aggtttaaaa attttcaaac ctacggggtt tttgaccctg
      481 aaataggttt tccaaaggtc aatattacct tgacttttct ttagtatacg tttttcggct
      541 tttgttgtca aacctttcta acatgaggtc gaagcttccc tgcaaagact tatgatcgat
      601 tgtgagagct cttttttcgt cttcgtcgtg tttcggtacc gtgccgcgaa ccgtcctcga
      661 aatggggtga acattccgtc cggactccgc gatagagccc atcgtatagc ttctatacgc
      721 caaaaagccg cctctgatga gacagctttt gtgtatgatg gtcgtattct ctatttatct
      781 tcgtagaccc ggacacgtaa ggtcagcccc aagtcgtcac agatggcccg gcattttttg
      841 atttcttcac tgtcttcgac tttatcgacg acggtgagga cgacgttttt cacgtactgt
      901 ttgcagctga cggcaaagtt cagcatggca tcgaaggacg gcaggccgaa tttgctccgc
      961 gtcagttcga gataccgttc tttgttggag gcgttcatgc tgatggaaat ggtatcgagg
     1021 atgccgccgc caaagtccgg tgccgtcggc cgaccgtagt ccaaatcgga caggccgttg
     1081 gtgttgagcc gcgtcttgac cgtcgggtgc ttttctttaa cgtatgccag aagggttttc
     1141 aagtcttcca ggcgttccgt cggttcgccg aacccacaga agacgacttc gttgatacgg
     1201 tcccaagggg ccccgtcgag ttcttttttg acttcatcga cggtcggttc ttgtttcagc
     1261 cacagcgttt cgtcttcagc catcttcttc atcgagcgca ggcagaaggt gcacgaacag
     1321 gtgcagcgat tggtcagatt gacgtacatg ctccacgggc tttctttctg taatgcccgg
     1381 tcgtctttta aatcgacgta ttgtccttgt cctactacgg tataaacgat catgatgcta
     1441 tcccctttta ttgtaatgtc gtcaaatatt cttcaaacgc aaggccggcg ttgtgcggaa
     1501 gatctaaggt ctctgtgtat tccatgacct tgcagatgaa gtcggccgct ttttgtacgg
     1561 cttcttcaag gccttctccg cgaacgacag aggccgagac gatggccgtg aagacgtcgc
     1621 ccgtgccgct gcggtcgctg ccgatttttt tcgtcttgac ccacgacgtt tggccgtgat
     1681 cgtaaatgaa gtttccaatg tacgaaccgc ggtggatacc ggtcaggacg acgccctgcg
     1741 gtcccttgtc ggccaaggcc ttggccatat cggaaaagtc atcgtcagat aagtcgctcg
     1801 agggccggta aggccgcccc aagagctcac aggcttcggt caggttcggc gtcaggacat
     1861 cggcatcggt gagaaggcgc cgcatatcgt cgcacatttc ctgggtataa gacgaataga
     1921 tgcggccatt gtctcccatg acggggtcga cgatgacctt cgtatccggt cctttgaagt
     1981 cgtgcagaaa atcgaggacc atgtcgatct ggcggaccga gccgagaaaa cccgtgctga
     2041 tgccgtcgaa ggtcagctta tttttcttcc aactgtcgat ataaggcgtc atgcggtccg
     2101 tatagtcgtc gaggtagtaa tcgtgaaagc cggtgtgaac ggaaaggatc gccgtcggca
     2161 ggatacagcc ttgtaccttt aaggccgaca cgatgggcag ggccacggcg atgctgcagc
     2221 ggccgtagcc ggttacgtca ttgaccaggg ccaacttttt ttgatgcata gagaatcatt
     2281 ccttatcttt tcaggttgta accataacca cggaacggga ctctcatgaa acaagcagcg
     2341 atggcgtaag cagcagccat gttgacgatc ccttccccga tgaggccggg caccatggta
     2401 aggccggctt ctaagctgcc gtagaggaga ccgccggctg ccgtatagga aacgaccatc
     2461 cagacggccg ataagaggaa gcccaaaaag accttgaggg atgtcagttt ccacggttcc
     2521 ttatcggggc gaacgaccca aaagacggct tcgaccatga cgaatttaat gatcagggtc
     2581 ggcacgatcc aaatgggaaa cccgccgatc aggtcggaca aggccgaacc gatggaagct
     2641 gcagccgccg cctgacggcg cgaactgaac aggacagacg caaagatgac ggcatcaccg
     2701 agatgggcat agcccaaggg aatggggatg cgcggtacga aagtcaggat aaagaccagg
     2761 gccgttaaga cggcagcata acagagattg gcagccgaaa aaaccggtgc cttttgttcc
     2821 atagatgatg aatgcatgtg aaatcctcct cacgggtaca gaattcacac attggccaat
     2881 gtatggttat gattaatctg tactctattt tagagtatat tactcc
//
LOCUS       DAWDXZ010000186         2915 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7129_length_2915_cov_7.470629, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000186 DAWDXZ010000000
VERSION     DAWDXZ010000186.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7129_length_2915_cov_7.470629"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    619..628
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1382..1391
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2507..2516
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          689 a          789 c          708 g          699 t
ORIGIN      
        1 tggcatcttc cctttcgcaa aaatattgat acatcgccat aaggccgggc atcagtttca
       61 ttttaggacg ccagttgagt attttctcgg ctttttcact atgcagtgcc gaacagtgta
      121 catcacctgg gcgtgcatct tcataagaga tagaaatacc ggcatgggta aaatacagca
      181 ggatgacttt cagtgcgttg atcgtcgtct ccatctgcgt gccgatatta tacgtctcag
      241 ccggcccctc atccgccatg gccgccagat tggcccggac gacgtcgtcg acgtagatga
      301 aatcgcggga ctgttcaccg tcgccataga tggtcatatc ttcgccgtaa gccacagccc
      361 gggtaaaacg gctgacaacg ctgttctcct caccctcacc atatacattg gcatagcgca
      421 aaatgacata tgacaggccg tacgcttcat gataccggcg gatacaatct tccgcagctg
      481 ccttagcctg tccgtcaaag ttgatggggc taagctcatc ttcttcgcga acgggaacgc
      541 ggacgggctc gccgtagacg gcagctgacg aggaaaaaat gaatttcttc acaccgtgac
      601 ggcagcactg gtcaaggann nnnnnnnnaa ccttaagacc tgtaaggctc cctatgacat
      661 cgttctcaga aaagtcctgc gaatcggaaa gcctccgctg cggtgcttca tgaaagacga
      721 tatcgatggg cttttcggaa aacactgaag cgacggcctt ttcatcaccg atgtcgcctt
      781 cgatgcagcg ggcttcgtcg ggaacctgag caaaggttcc tttggaaagg ttatcaaagg
      841 ccgtaacctc atggcctgct tcaagcaggg ctttgacaag gcgggacccg ataaagccgg
      901 cacctcccgt aactaaaata tgcaaggcaa gtcctcccgg accttacggc aaatcgccgt
      961 aggtaatgag ttgaatctct cttttttcga tttgacggcg gatcgccggc gacaaaagtg
     1021 cctgaaattc atcctgccag tggtactccc aggaaaagct ccggctaaga accgtcgtac
     1081 tgcgtcccgg atgcatcatg atttcatgga ctccgaaggg cagccggtcg atgagatact
     1141 tcaagttgcc ttcgttcatg ttgccgccgt ctaccatgcc ccaaaagaaa tctgtcgtcg
     1201 taaaatgaag gcgtttcacg gcatccatgg ccttctggga caagtaggtc aggccgtcgc
     1261 ggccggcgat gcgcacggga ttggccgaaa acagcttata tgaataagct tctctcggaa
     1321 tgcgtactcg gtggataccg tacttcatca tcagcgactg gacgatgttc cagatctgcg
     1381 gnnnnnnnnn nccggtaccc gtccactgtc agtcaccagg gtaggtactt cctgcgggga
     1441 caaaacggtg ggcaacgacc cgacgaggca taagtggatc ccgattccga gacggggaca
     1501 agtccgagcc agcgagacgg cttcttcgaa agccggcccc gttggcaata aggacgtact
     1561 ggtcagaacg ccctgttcac aagcctcaat aatggcatga ttcacttccg tatgaattcc
     1621 gaaatcatcg gcattaataa tgactctatt catcttttac gctccctgtc ctttcaggat
     1681 gcactgcaca tcggcttaat gactattata cacggacaat cgtattttga caatatcctc
     1741 cccgggacta ttcggcaaat tggcggtatg ccgcttcctt caccttgcgc tgccattctt
     1801 tcaaagggat gcccgtcgct tctgccgccg cccgacagtc gtcgtattcg gcggaaatat
     1861 tgacaatctg tccgccgtac cggcagattt tacagtgaag atccctgccg tcaatgcaga
     1921 ctgtcataat gatccggtca caggccatgc ggctcacggg aaaaaagcga acgccaatag
     1981 atgtcgtctc tttaacgatc gtttcaatca tatcgtcgag gagttctttt cggcaaagaa
     2041 cacagagcat cgatgccatc cgcccttttt tcatcatcat cggcatagcc cagacgtcat
     2101 tggccccctt gtcgagaatc tgtgaaaaga cgtattccat attttgagga ttcatatcgt
     2161 cgatattggc ttccaattgc cataaggttt ccatcggctc acctcagact gttcatcgta
     2221 tcggccagtc tgccggcgcc gaaaccattg tcgatgttga cgacagatat cccggcagcg
     2281 cagctgttga gcatagccag aagggccgaa agaccgtgga aattggcacc gtatccgata
     2341 ctggtcggta ccgcgatgac cggcttgtct actaaacctc cgacgacact ggccagagcc
     2401 ccttccatgc cggcaacgac gatgttgaca ttggcttttc ggatatcgtc gactcgtgcc
     2461 agcaatcggt ggatgccggc aacgccgaca tcggagattc gttccannnn nnnnnnaatt
     2521 tctgccgtaa gcgccgcttc ttcagcgacg ggaatatcgc tggtaccggc cgtaatgacg
     2581 gaaatgaggc gatcgccgtt gatgatggtc ttatccttgt gctgatagac catcttggcc
     2641 agctcgtcgt attgggcagc cggaaactta gccgataaga tgtcaaaaat ttctttcgaa
     2701 gccctcgtgc cgatgacatt gtcatatttt tccatcaaaa tttctaaaat ccgtacggac
     2761 tggtccgtcg tcttcccggc acaatagacg acttccggaa agccgcagcg ctcttgacgc
     2821 tgtaagtcga gttttgcaaa accaagatca agggtgtcct tattttcttc catgattacg
     2881 cctccagact atacgtgaaa accatttttt tccca
//
LOCUS       DAWDXZ010000187         2913 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7138_length_2913_cov_6.726732, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000187 DAWDXZ010000000
VERSION     DAWDXZ010000187.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7138_length_2913_cov_6.726732"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2175..2184
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          672 a          770 c          695 g          766 t
ORIGIN      
        1 ataaggccaa tgatgacatt gaccacgaga tggcgcatag gcgtcaaaat caccttatgc
       61 accaaatata agataacgac gccgatggct atcgacagat attccatacc ttcctcacat
      121 ttcctgccga ttggcaacgg cacctgccag tgtcgcttcg tcggcatatt cgatatcgcc
      181 gcccatagga agtccgcggg cgatacgagt tacgcgaatg ccggccgtct tgatgagacg
      241 cgacaaatac aaggccgttg cttctccttc cacatccggg tcggtggcca agataacttc
      301 ctttacatcg gtattgccca aacgctgcag caattctcgg atgcgcaaat cctcgacatt
      361 aactccgtcc aaaggagaaa gggctccgtg aaggacgtga tataagccgt ggtaatcacc
      421 gcttcgctcg atagcttgga cgtcctgcgg cgtttctacg acgcagattg tcgaatggtc
      481 gcggctggta tcactacaga tagggcaggg gtcctgagaa gacaagttga agcaaaccga
      541 gcaaaagtgc atattttcct tagcatcgcg aatggcattg ataaattcat cgacgcggcc
      601 cgtcggctgc tgaacgatgt aataggccag cttccgagcc gttttaacgc caatccccgg
      661 caggcagcgg aactgctcgg taagcttgtc taacggcgta tccattagaa cattcccggc
      721 aggttcatcc cgcctgtaat cttgctcatt tcctgagcca tcatgtcgtc aactttctta
      781 ttggcttcat tgacggcagc taccatgagg tcttccagca tttctgcatc ttccgggtcg
      841 atgatgctcg gatcgatgtg aacgctttcg acattttttt caccgttcat gacgatggtc
      901 aggactccgc cgccgaccgt cgcttctacg gtgcgttttt tcaaatcttc ctgcagtttc
      961 ttcatttcct tctgcatctt ctgtactttt ttcatcatgc cttgcatatt tccaccaaac
     1021 atttgatatc ctcctgtttc tttcaagctt attttttatt ttctatatat atattacaat
     1081 ctcccaccgt tttaatcaat ggcccgagag cggccctttc atcggcgttc atgtcgtcga
     1141 cagaggccgg ctgccacact tcttcgtcgg attcgggcaa ggctgtagga gcttgaatat
     1201 cggtttcagg tatttccgac agcggttcag gttccggttc cggaggtcgg acgacttccg
     1261 ggacttcagg gacgaccgac gctgccgcta cggatttatg cttttttttt tcgtaagtct
     1321 tcagctcatc gtccccttca aggaacccct gcatatgata ggcccctccg aggacagaag
     1381 acaaggcttc atcggtaagt ttaaagtaat cttcgcgatt cgcccgttta accatgaagg
     1441 ccgtcttaaa ggcgacgata acctgcccat cgccgatgta aacgacgcgt ccattgcgga
     1501 tacaggatag aacagctttc ttcttctctt tgtcgaggat ggcacacatc ttatcccaaa
     1561 cgttctgata atcagccgat acgacaggag ctgcatccga agctgccgac tgaaccggcg
     1621 ccttcgtttc aggcacattc tgatgagccg gcactgccgt ctgttctgcc gatggagaag
     1681 tcggcttttg attggattgc cttgagggga cagccgcaaa caaagcatct tttccggaag
     1741 ttcgttcgac agacggcgcc attcgttgaa ccgatgtaaa ctcatcatcg ggaaagggaa
     1801 cggcttcttc cggaacacta aatgattgat aatcgtcttc ttccggtacg gggatcgcct
     1861 cgtgatgctg ctttttaggc gccggctgca ggatatgtgc ccccgtatca acagcagtta
     1921 caggctgtgt taatttacgg gccgccatga gcagccccat ttcaacggca atgcgcggaa
     1981 ccggcgacgt tttcgccgtc agtaaagcct gctgcaacac atcgacgact tgaaaaatag
     2041 tttccggcga ggcagactgc gcttgtttac gaagttcccc catgctgtca ctataagccg
     2101 aaagcgtatc tgatccgggt gctgcctgac agagcatgag agcccttaaa tgggctaaaa
     2161 tctcggtcag gatcnnnnnn nnnnctttcg tcagttcaat ctgttcctgc tcggcaacgt
     2221 agagcaggcg atgggcaatg tcagcactcg taatacggcg aaattcatat cgctggcacc
     2281 gcgataaaat agtcagggga atccgttccg gttccgtcgt cgccaaaata aagatgacgt
     2341 gagccggcgg ttcttctagt gtcttcaaca gagcattaaa ggcttctgtc gtcagcatat
     2401 gaacttcgtc gatgatataa attttcttat gcccttcaac aggcataaat tttatcgttt
     2461 cccgcaaggt gcggatatct tcaatactgc ggttagaggc ggcatcgatt tcgacgacat
     2521 ccaaggattc gccgctcata atatggagac aggcttcaca ggcattgcag gggtgagccg
     2581 tcggtccatg aacacagttc atagcccggg caaagatttt ggccgtactc gtcttgccgg
     2641 tcccgcgagg gcccgaaaag agataggcat gagcaacctt gtccgtctct atcgcttttt
     2701 gcaatgtctc cgtaatatgg gactggccga caacatcttc aaatccctta ggcctccatt
     2761 tacggtatag tgcaatgtat gccatcgata cctttccttc cgtatatgaa aaaaagcagc
     2821 taaccgctgc ttttaccaac tgaaaagtac tcgtgtatgg taacccgcca ggtttcctcg
     2881 tggcacatgg aaagatctac ttattgctgc ttc
//
LOCUS       DAWDXZ010000188         2912 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7142_length_2912_cov_7.039202, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000188 DAWDXZ010000000
VERSION     DAWDXZ010000188.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7142_length_2912_cov_7.039202"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    529..538
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1200..1209
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2025..2034
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          774 a          740 c          790 g          578 t
ORIGIN      
        1 agcggcagaa agacgttgta gacggcatta atataaccaa ctacgacaaa ctagaccgct
       61 tcgacacgtc agtattttcc ggggtagtct tggatgagtc gtccatactc aagtccttca
      121 ccggcaaagt acggacgatg ctaattaatg cgttttcccg gacgccgtac cggctggcct
      181 gcacggcaac gccagctccg aatgactata tggagctggg caatcactcc gaattcttgg
      241 gagtgatgtc tcgtacagaa atgctgtcga tgttttttgt ccatgatggc ggcgacaccg
      301 ctaaatggcg gctcaaaggc catgccgaga tggccttctg gcgatggatg gccagttggt
      361 ccgtagtcct cgacaatcct actagtctag gttatgcgga caacggctac gagttgccgc
      421 cgctcagagt ccatgagatc atcgtcgacg gcgatgcgcc gacgaccgag aaactgacac
      481 tgactcaaag gcgcaacgcc cggaaagagt cactggatgc acgttgtcnn nnnnnnnnac
      541 tcccaggtca ccgtcttcag atacgagagg caaccgttac cggcaccaaa ttggatagga
      601 ccaaacatca cactaccacc aggtccgcgg gtccgacaag gccagctaca aagcaagtac
      661 gatgcttgga ttttcggtag gcgtcctgaa atgcctggtc actaagccca aaattgcggg
      721 cttcggcatg aactggcaga attgccggaa catgattttt gtcggattat ctgatagcta
      781 cgagcaatat tatcaagctg ttaggcgatg ctggcgattc gggcagactc agcctgtgga
      841 cgtctacatc atcatcagcg cccgcgaagg cgctgtaaaa gagaacatcg agcgcaagga
      901 agcggacgcc atcaacatgc gccgtaagat ggctgaactt acaagggaat ccgtccggga
      961 aaacctaacc cggacgacta ggataatggc ggagtataaa cctacgacgc cgatgagatt
     1021 gcccaaatgg gcagaaatgg agagcgcata gatgaaaaag atttatgttt ctcaccccta
     1081 cgggggctta gcggacaaca agaaagctgt agaagactta ttagaatctc tcgtgaatgc
     1141 agaaaccgac attgcgggaa gcaccttatt gagtcttgga tgcgacacca aatttatttn
     1201 nnnnnnnnnt tatataacgt cctggattat agagcagggc ttaatatgtg cctgaccttg
     1261 ctgagcaagt gcgatgtcct gctcctgtgc ggcgattggc agaattccaa gggatgcatg
     1321 gcggaatatg cctacgccaa ggctaagggc atccccacga tgtacgccgg aggaaaatat
     1381 gactaacgta ttaggccaat acatctctag ccgagtatcg ctatataacg gcgattcggt
     1441 ggagatgctc agaggtcttc ccgacaatag catacattac agcattttca gtccgccgtt
     1501 ctcgtcgtta tacacttatt ccaacagcga tagagacatg ggcaatagcg caagcgatgc
     1561 ccagttctat gagcacttcg gatttttaat ccatgagttg gcacgcgtca tcatgcccgg
     1621 ccggttagta tccgtgcact gcatggatat tccacgcatg aaatcccggg acggggtaat
     1681 cgggcttaag gatttccccg gcgagattat ccgggcgttc gaggcggcag gatttatcta
     1741 ccatagccgt gtcgtcgttt ggaaggaccc tcttgtagag gcgacccgaa ctaaggccct
     1801 ggggctgatg cacaagcaat tgtgcaagga ttcggccatg tgccggaacg gtttgccgga
     1861 ttacgtggta acgttccgca agcccggcga caaccccgaa ccggtcgaac atgaggacgg
     1921 cctgaagcgg ttttacggcg aggacgagcc ggaaggcgta cgaacactgc gaccgcagcc
     1981 cgatccggaa ctcgtcgacg caaaaaagaa gtacaacacc atgcnnnnnn nnnnatgccc
     2041 atctatagcc atcaagtatg gcgacggtac gcgtcgccgg tatggtcaga catccggcag
     2101 agtaacacgc ttaaccgggc cgcggcccgc gatgagaagg atgagcggca tatctgcccg
     2161 ctccagttag atcttatcgc caggtgcttg gagctgtgga ctaatccgga tgacatcgtc
     2221 ctggacccat tcgcgggcat cggtagcgtc cctgttatgg ccttgcaaat gggccgcagg
     2281 gcaataggtt tggagttaaa ggaatcctat tacgcgcaaa tgatactgaa ttgtaaaaaa
     2341 gaggaggaaa cccaaaatga tgaagatcaa cattgaagtc agcggagaag ttaacgaaat
     2401 catgcaggaa atccacacgt taggcggaat cctgattgcg gcgaacaatc acggagaaga
     2461 tgtgactgtg gcatataagt cggaggagcc caaagccgcc gaaaaacctg cgaagaaaac
     2521 acatcgaaag aaagaaacgg cctcagtaga gcaaccgcca aaagcggatg cagaaccgga
     2581 aaaggccgct ccagctccgg aaatcgtagt acctgctccg gcagctccgg ccacggaacc
     2641 tgtagctgct gcgcagctgg cacctgccgc ccagattccg acagccccgg ccaaaaaata
     2701 taaaattgaa gacttattaa cggcgacagc gccgctcatg gatgcgggca agcttaccga
     2761 gctgcaagcc ctgatgcaga aatacggcgt ccgctccatg atggaaatcg cgccggaacg
     2821 atacggagaa ctggcaacgg atctccgcgc attgggggcg agaatctaat gagccgacaa
     2881 catgcacttt tgagcgcctc atcggcgcac cg
//
LOCUS       DAWDXZ010000189         2905 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7155_length_2905_cov_9.848421, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000189 DAWDXZ010000000
VERSION     DAWDXZ010000189.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7155_length_2905_cov_9.848421"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          687 a          879 c          789 g          550 t
ORIGIN      
        1 ccgagcgcaa gcacgtcgcc atctacccgg ccagccatta cgtcacgacc aagaacaaga
       61 tggaacgggc cttgggcacc attagcaagg aattggacga acgattgaaa gtactcaaag
      121 acggggaaca cctgttggaa gcccagcgcc tggaacagcg gacgcggtac gacatggaaa
      181 tgatggaaga aatgggctac tgctcgggca tcgaaaacta ttcccgccac ttggccggcc
      241 gtaaagccgg acaggcgccg tacaccctcc ttgattattt ccctgacgac ttcatgatcc
      301 tcgtcgacga atcccacgtc accctgcctc aattgcgggc catgtacgcc ggtgaccggt
      361 cgcggaagga aagcctcatc gaatacggct tccgcctgcc gtcggccctc gacaaccggc
      421 cgctgaagtt cgatgaattt gtcgaacgca tcaatcagat cgtctacgtc agcgccacgc
      481 ccgggccgta tgaaatggat gtcgaaacga atatggccca gcagatcatc cgcccgacgg
      541 gcctcctcga tcccaaggtc gaagtccgtc ccatcgaagg ccagatggat gacctcttag
      601 gggaaatccg caagcggacc gacaaggacg aacgagtcct ggtcacgact ttgaccaaga
      661 agatggccga agatttgacg gactatctga aggaaatggg cgtccgcgtc cgctacctcc
      721 attcggatat cgctaccatc gagcgggctg agatcatccg cgacctgcgg gtcggcgaat
      781 tcgacgtcct ggtcggcatc aacctcctgc gcgaaggcct ggacatgccg gaagtctctc
      841 tggtggccat cctcgatgcc gataaagaag gcttcctccg ttccgatacg tccctcatcc
      901 agaccatggg ccgggcggcg cgaaacgtcg aagggacggt catcatgtat gccgataaga
      961 ttaccgattc catggcccgc gccatggacg aaacccagcg ccgccgggaa attcaggata
     1021 cttataataa acagcaccac attacgccga aatcggtcaa gaaagacgtg gtcaacctca
     1081 tcgaactgac gaaagttgct gaagacgatg cggcctacgg cagcaaacga ggcggcaagg
     1141 gcaaggccat gacccgcgaa gccttgaaca aggtcattaa gaccatgacc ctgcaaatga
     1201 aggaagcgtc gaagcggctg gaatttgaaa aggctgccga actgcgcgac cgcttagggc
     1261 agctgcgcca acagttggcc gatatgagcc acgacgacac cctgccgaaa gaaatgggcg
     1321 gggaagacat gcccagccgc cgctaccggc gaaagatcaa gcggcccgat gctaagaaga
     1381 agcgctaggc cgctgcggta ggaagataga ggaaggagtt taccttgcaa gataaatata
     1441 ttgtcatcaa aggtgcccgc cagcacaatt taaaaaatat cgacgtcaag attccccggg
     1501 acaagctggt cgtctttacg ggcctgtcgg gatcgggcaa gtcgtccctg gctttcgata
     1561 ccatctatgc cgaaggccag cggcgctacg tcgaatctct gtcggcctat gcccgccagt
     1621 ttttgggcca aatggacaag cccgacgtcg actatatcga aggcctgtcg ccggccattt
     1681 ccatcgacca gaagacgacc agccgcaatc cccggtcgac ggtcgggacg gttaccgaaa
     1741 tctacgacta cctgcgcctg ctctatgccc gtgtcggcga cgctttctgc ccgcattgcg
     1801 gcaagcccat tcgccagcag accatcgagc agatggccga tgccgtcatg gccctcggtg
     1861 aaggggagaa ggtcatggtc atgggccctg tcgtcgacgg ccgcaagggg acccatcaaa
     1921 agctcctggc ccgactgatt aaagacggtt atacccgggt ccgcgtcgac ggcgaagtct
     1981 acctcctgtc cgacgacatc cccttagata agaataagaa gcataccatc gacgtcatcg
     2041 tcgaccgctt ggtcattaaa gacagcatcg tcatgcgtct caccgattcc ttggaaacgg
     2101 cagccaaact ggccgacggc atcgtccaca tcttccgcat ggggactcag gaggtcctga
     2161 ccttcagcca gcactttgcc tgcccggact gccatatcag cctgccggaa atcgagcccc
     2221 atctcttttc tttcaacagt cccttcggcg cctgcccgga ctgctcgggt atcggcagta
     2281 cgatggaagt cgatgcgcac ctggtcttgc cggacaagaa gaaatccttt gccgacgggg
     2341 ccattgccgc cctcagcagc aatcccgatt cgtggttcat gcgtcagctc ggcggcctct
     2401 tgaagcccca cggctttacc ttggataatt gttacgacga cttgccgaaa gaccttcagg
     2461 atcaattgat gaacggctcg gacgaaaact gcgtctttga atatgaaaac ctgcggggcg
     2521 aagtgaagac cttttccctg ccttttgaag gcgtcctgcc catggtccgc cgccgttacc
     2581 gtgaagcctc ttccgatatg atgcgcgacc agtttgccca gttcatgacg gtcaagccct
     2641 gctcgacctg tcacggcacg cgccttcgcc acgaagctct ggccattaaa atcggcggcc
     2701 tcaacattgc cgagctgacc gatttgccca tccgcgacat gattcctttc tttgacaatc
     2761 tgaaactgac agaaaagcag cagcttatcg gccagcaggt ctttaaggaa atcaaggctc
     2821 gcctggcatt cctccagacc gtcggcctcg attacctgac cctgtcgcgg acggcaggca
     2881 ccttatccgg cggcgaagct cagcg
//
LOCUS       DAWDXZ010000190         2903 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7163_length_2903_cov_6.701545, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000190 DAWDXZ010000000
VERSION     DAWDXZ010000190.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7163_length_2903_cov_6.701545"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    897..906
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2242..2251
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          894 a          679 c          542 g          768 t
ORIGIN      
        1 cattcaagta ttccataata aggcctccta aattttaaat ggttctaact agtgagctgc
       61 atggggaatc gacaaatctt tatgcttctt gccaacccct tttacccaat aagcaacaat
      121 aatcggtaca acaatcgaag taacaacaac acttgttgca accaaggcag tcgccgccgg
      181 aacaatttct tggaattcag gaatcatttg gccaacaatc atggggttgg taacagcggc
      241 cccggccgta ctcgaagcag ccaatccggc agccccattg ccgccaccaa taacacgatc
      301 agccagtata agaggaatac ccgtaaccac aataaccccg agacccagca taatgccaag
      361 aaggccagtc ttgaaaatga ccgtaaggtc aatcgtattg cctaaagcaa atgcaaagaa
      421 gggaatcatg atggtagcag ccggcgtaaa gaaatcacgg agttccggat ccaggttgcc
      481 aagaataaag ccaataataa acggaagaac agcacccaca aaaaggtgtg gttcaaaata
      541 tgcagcaccc gtcgaaccaa gaatcatcat cgtaacgagc gggcccgatt cgatagaagt
      601 cagtacaaaa gcaccggctt cttcttttgt cccgtattgc tgcatcaaag atgcatacag
      661 accaccgttt gtcatatcca tagaagtaac aatagctaaa gtggacaacc cggcaaagaa
      721 tccggtctgg atcatccctt ttggcatgaa agcgacacaa atgaatccaa caatccaagc
      781 tacaataatt tttgtaacta caatggtacc cgatttccga aggacgagac cggcattttt
      841 atagttaatg ctggcaccca tgcagaagaa ccataccgac agaattggca ccgtacnnnn
      901 nnnnnnatga attcctccct gctaaaaatt tatttcatat ttttaatttc aatgtggtcc
      961 atatcgtcaa ttctttattt tcgccgccca tagcccagat aaaggtgtaa ttatgcgtac
     1021 cgcagccaca gtgcatgctc caagacggac tgataacagc ctgttcatta tgcatgacaa
     1081 ggtgtcgcgt ttcctgttcc tgccccatga tatggaatac agcctgatct ttaggtactt
     1141 caaaatagaa ataaatttcc atccggcgtt catgtgtatg cggcggcata ctgttccaaa
     1201 cactgccttc gtcgagagcc gtaagtccca tgcaaagctg acatgtttca acaacatcag
     1261 gatgaataaa ttgattaata acacgtttat ttgctaatgc tgcgtctccg caaggtcttt
     1321 tattcgcatc tttaatggaa ataaatttgg tttggcaagc cttatgagcc ggtgcagaaa
     1381 ccatataata cttagccggg ttgttaggat catcagaagc aaaagtaact tttttcgtcc
     1441 ccatcgtgat atacagacag tcttttggtc ccatgttata agtctgaccg tctgctacaa
     1501 tggtgccagc accggttccg acattaaaaa caccaatttc acgacgttca aggaaatagt
     1561 gcgtaccgaa atttttccag caatcaatcc ctttatcaat aggaacaact tcattcaccg
     1621 gcatgcagcc aaccgctacc atacgatcaa catgagaata agtagccata acttcatcgg
     1681 gacggaacaa ggtgtcaata aggaattccc gacgaatgtc ttccgtcgtg tactttttaa
     1741 aatcctgcgg gtttgtagaa taacgaatat ccataataat acctccgaac aataataaat
     1801 aatttatttt aaggatagat aaggcaaaac atccttattc actaaatagg ttgggactcg
     1861 gccttgtaag acttcaataa ttcccatagc cgctttggtc ttcatttctt tatttgattc
     1921 ctcagaatac caagaaacat gaggagtcaa tacaacatta tccattgaca ggaatccatt
     1981 atctgatcga atcggttcaa cttccagcac atctaaaccg gcccctgcaa tttgatgatt
     2041 cgttaaagcc gtttgcagtg ctttctcatc caccagcgga cctcgtgccg tgttaattaa
     2101 aatagcagaa gctttcatca accccagcgt atccgaatta atacagtgat acgtttcttt
     2161 tgtaaggggt gtatggagag atataatatc tgcttcacga agcaactgat agaatggctg
     2221 gtataagacc cccatcgctc tnnnnnnnnn naagaacctc tagcccaaga gcctgagctt
     2281 taacggccac ctcgcgagca atcttcccaa aaccaaccaa gcccagttta tgccctcgaa
     2341 ggcgccaaat aggctgtgca atcgcataat cccaatgccc ttgatgaatc gagttatgaa
     2401 ggaaagtaac tcgccgccaa caatcaagca ttaaagcaat cgcttgcgta gaaacttcat
     2461 caatactgta atcaggtaca ttggcaacac aaatatggtg tttggtagct gcctccaaat
     2521 caacgttatc cactccgaca ccatagcgag caatcacttt taaattagga agactgctca
     2581 ttatcttttc agtgatatgg gcatattgaa ccatcaaccc ggctgcgtct ttgcagttct
     2641 ttataatgtc attttctgaa tgactttgag tcggtataaa gggaatacct atctcacgaa
     2701 acattttttg ttctatctcc aaggagggat agtcgtattc agtgatatac accgtatcca
     2761 taaattgtca cccggctttc cagaatggac ctcattcacg cattgcattc ttttatatga
     2821 taaatcaaaa aagtcatcat atgtatacgt ttaaattcat caaatgtttc ttgtgattgt
     2881 aatagcattt tccagcttaa tta
//
LOCUS       DAWDXZ010000191         2893 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7181_length_2893_cov_5.959831, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000191 DAWDXZ010000000
VERSION     DAWDXZ010000191.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7181_length_2893_cov_5.959831"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1507..1516
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          605 a          912 c          709 g          657 t
ORIGIN      
        1 acggtgcgtc gcgaatgcct ggcaggtctt acgaccttcg tctccatggc ttacattctc
       61 ttcgtcaatc ccatcatctt aggcgatgcc ggcatggatc ccggtgccgt ctttacggcc
      121 acagccttat cggccatcat gggctgcctg ctcatgggct ttttggccaa ctaccccatc
      181 gccattgccc cgggtctggg cgacaacgcc tttttcactt attccatcgt cctggctatg
      241 ggaatgccct ggcagcaggc catgggcggc gtcttcattg catccatctt atttaccttg
      301 gtatccttat tcaaactgcg tgaaatcatc atcgacgcca ttcctcagga cttaaagtac
      361 gccatggctg ccggtatcgg catcttcatc tccttcgtcg gccttcaagg cggcggcatc
      421 gtcatggccg acccgtcgtc gatggtcgcc atcggttcct ttaaggtcgc aacgacgtgg
      481 ctgactattt tcggtatctt cgtcacggcc atgctcatgg ctaaaaaggt tcccggcgcg
      541 attttttacg gcatcgtcct gacggctatt ttgggccttg tcactaatct catccccttg
      601 ccggacagca tcatttccat ggctccgagc ctggaaccga ccttcggcgt cggcgtcgcc
      661 aacatgggcg ccatcgttac cgacccgcag atgtgggccg tcgtcgtcat cttctttctg
      721 gtcgccttct tcgatacggc cggaaccctg attggtcttg cccagcaggc cggcatcatg
      781 caaaacggca agatgccccg catcgggaaa gccctcatgg ccgattccct gtccatgctc
      841 ggcggggctg tcatgggaac gacgccgacc gcagcttacg tcgaatcgtc ggccggtatc
      901 gccgtcggtg cccggacggg ccttacggcc attgtcgtcg ccattctctt tggcttcagc
      961 atgattttct cgcccctctt gtcggtcgtt acggctcagg tcacggcacc ggccctgatc
     1021 atcatcggcg tcctcatgac gtcgtccctc cgcgaaattc attgggatca attcgagacg
     1081 gccttcccgg ctttcttagt catcatcggc atgcccctga cctttaacat ttcctacggc
     1141 atcgccttcg gcttcctctt ctacccgatt ctgatgacca ttgccggccg ccggaaagaa
     1201 ctgaatcccc tcatttgggt catgttcgtc ctgttcctca tcttattgta tactttgacg
     1261 atacttaaat tctaaggagg tctgtccatg cttacaaaag aattattatc cgactacatc
     1321 gatgccggcg cggccaaaat taaggccgac ctggccatcg tcggcgttac attgatcaac
     1381 gtcaacaccg gcgaatacta caaagccgac gtcgccatgt ataagggaaa aatcgtcgcc
     1441 gtcgaaagtg atatttccga ttccatggga cctgacacca aaaccgtcga cgggacggga
     1501 aaatatnnnn nnnnnnatat ctggcaccgg gcctcatcga ctgccatatc cacgtggaat
     1561 gcagtaaaat gagcatgact cgctttgctc aggccgtcgt cccctgcggc acgacgagta
     1621 tcgtaagcgg ccttgacgaa tatatttccg tcatcggtgt cgacggctta ggtccgattt
     1681 ttgaagaaat cgaagcgtcg ccgctgaagg tcttctgggc cctgccgtat aagacaccgt
     1741 acaccattcc cgtgtcgacc atcgcctaca atgtcaccgc caaagaccac gctaaatacc
     1801 atcaggatcc gaactgctac ggcatttggg aactcgtccg cgaagccgtc cagacgaagg
     1861 attcggatac gctggaagcc ttggtcttag cccagaaata ccatcgcccc atcttcggct
     1921 gcagccctct ggcccgcggc aaggacctta acgaatacct catgagtggc gtccgcgtcg
     1981 accatgaaag ctacgaccat caggaatttt tagaaaaggc ccgcaaaggg atgcacacca
     2041 tcatccgcga atcggcggtc accaagttcc tcaaggaaaa tgtccgggcc atcactgaag
     2101 gggccccggg tattgcccgc cacaccagct tttgctcgga tgacgtccac gcccgcggca
     2161 tcttaaagac cggccatatc gacaacatgg ttcgcctggc catcgctgcc ggcctcgccc
     2221 cgatgacggc tatccagatg gccaccatca atgccgccga agcctatgaa atcgacgacc
     2281 gcgtcggttc catcgccccg gggaaagatg ccgacatcct cctcgtcgac cagcccggga
     2341 cctttaccgt cgaaacggtt atcagtaagg gcatggtcat cactgaaaac aagaaaaacg
     2401 tcttcaccta caccgtcccc ctccgcgatg cagccttgtt acatacggta cagcacgaaa
     2461 aaatgcagcc tctcgacttt gcttatcacg tcgacctcca agaaggcacg gccatcgtcg
     2521 aaaccatcga cagcatcggc cccttcatgc gcaaacgccg tgacgtcgaa ctcgaggtca
     2581 gagacggcat cgttcgcccg agtgccgaaa aagacgtggc cttagtctcg gtcatcgaac
     2641 gctatggtaa gaacggcaac gtctccaaag gcttcatttc cggctggtcc ctcaccaaag
     2701 gtgccattgc cacgacggct gctcccgatg acaataacct cgtcgtcgcc ggcgctaact
     2761 acgaagatat ggcattggca gccaataccc tcatcgaaaa cggcggcggc caggtcgtcg
     2821 tcctcgacgg gaaaatcgta tccttcctgt ccctgccgat tgccggccgc gcctccgata
     2881 tgacgccggt aga
//
LOCUS       DAWDXZ010000192         2884 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7203_length_2884_cov_8.808413, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000192 DAWDXZ010000000
VERSION     DAWDXZ010000192.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7203_length_2884_cov_8.808413"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    234..243
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    900..909
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1493..1502
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2349..2358
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          616 a          855 c          710 g          663 t
ORIGIN      
        1 tgtacttatg atgcttcatg atattggcgt cgtcggcatg gctgttatgg gcagtaacct
       61 ggccctcaac atggccgacc acggttatga cgtcgctgta tacaactaca cgccggattt
      121 gacggaaaaa ttcctcgaag aacggcctca cgataagatc catggttttt tagaactcga
      181 tgaattcctg gcctccctca aacggccgcg caaaatcatg ctcatgatca tggnnnnnnn
      241 nnncctgtcg acagcatgat cgaccagctc ctctcgcgcc tcgataaggg agatatcatc
      301 atcgacggcg gcaactccta cttcggtgat actcagcgcc gctgcgaacg ctgccaagaa
      361 gcggggattc acttctacgg catgggcatt tccggcgggg aaacgggagc ccgccgcggc
      421 ccggctatca tgcccggcgg caataaggaa acgtacgccg aaatccagcc catttatgaa
      481 gccattgccg ccaaagcctc ggacggcaag ccttgctgca cctatatcgg ttccgacggt
      541 gccggccact acgtcaagat ggttcacaac ggcatcgaat acgccgatat gcagctcatt
      601 gccgaagctt atttgttgct gaaatacgtc ggcggctatg ataacgcggc tatctccaag
      661 attttccacg actggaacca aggggaactc aagagcttcc tcatcggtat cgccgccgat
      721 atctttgccg aagacgacga agacggcggt caggtcctcg acaagatcgt cgacgccgcc
      781 ggccagaagg ggaccggtcg ctggaccagc atcgaatcga tgaaacaagg cgtcgatatc
      841 tccatgatta cggcagcctg caacgcccgg gtcatgtcca atgcaccgag ccgtgtcaan
      901 nnnnnnnnng ggacgatttc atcgaagccg tccgccagag cctgtacgcc gctaaaatcg
      961 tcgcctacgc ccaaggcttc tccctctata agagcgcttc ggaaacgtat gactggcatc
     1021 ttgattacgg cgccatcgct tccattttcc gggccggctg catcatccag gccgaatttt
     1081 tgaccaagat caccgaagct tacggcaaga atcccgacct cgacaacctt ttgttcgacg
     1141 atttcttcct caaaaaaatc aatgacaacc aaggtgccct gcgccgcatc atcggcctcg
     1201 ccgtcagtca gggacttccc attccggctt tctcggcagc actccagtac gtcgatgcct
     1261 acagcagccc taaggttggg gctaacctca tccaggccct gcgcgattac ttcggagctc
     1321 atacctttaa gcgcgtcgat aaagaaggta ccttccatca tcagtggcac gaacactata
     1381 cgaaataaag ggggtttttc atcatgccac tcgtcatcct tgccattggc attctcatct
     1441 tatttttcct cattgtaaaa gtaaaactca atagttttct ggccttgctc ctnnnnnnnn
     1501 nnggcctcgt cggccttgct gaaggcatgc ctatcgatcc attgatcccg accattgaaa
     1561 agggccttgg cggtacctta ggcggccttg ccatcgtcgt atccttcggc gccatgctgg
     1621 gcaagctcat ggccgaaagc ggcggcgccc agcgcatcgc cacgacgctg attaacgtct
     1681 tcggacggga aaaggtaaaa tgggccgtct gcctgacggg cttcatcgtc ggcatcgcct
     1741 tgttctatga aatcggcttc gtccttctca ttcctttagt ctttactatc gccgcggaag
     1801 cgaaaatccc tctcctcgaa gtcggtatcc ccatggcagc ggctctgtcg gtcacccacg
     1861 gtttcctgcc gcctcatccc ggtccgacgg ccattgccat catctataat gccgatatcg
     1921 gcacgacctt attgtatggc gctgccattg ccattccgac ggccatcgtc gccggcccct
     1981 tgtactgcaa cttgaccaag gatatcaatc cggaaatccc caagggtatg tacaatccga
     2041 aaatctttaa ggacgaagaa atgccaagct tcggcatctc cgtctttacg gccctggttc
     2101 cggtcatcct catggccgca tcggctatct ctaaattgac catggatgct gatttgcccc
     2161 tgcgtcacgt cctgaccttc ttcggccagc ccgatattgc cctgaccatt tcggtggcca
     2221 tcgctatcta taccttcggt atcggccgcg gcaaatcgat ggaagacgtc atgaagaccg
     2281 tacaggatgc ggtccttgcc attgccatga ttttactcat cgtcggcggc ggcggtgcct
     2341 tgaagcagnn nnnnnnnnca tcgacagtgg cctcggtctt tatatcgggg aacttgtcgc
     2401 taactccgac ctctcgccgc tcttcctggc ttggctggta gctgccgtca tccgcacggc
     2461 ctgcggttcg gccaccgtcg cagccctcac ggccggcggg attgccgctc ctattgcggt
     2521 cgctaccggt gccaacccgg aattgatggt cctggcaacc ggtgccgggt cactcatctt
     2581 cagcccgccc aatgatcccg gtttctggtt gttcaaagaa tttttcggct tgaccatcaa
     2641 agaaacggtc cgcacgtggt gcgccctgga aacgatcatc gccgtcatgg gcctcatcgg
     2701 cgtccttgtc ttaaacgtat ttgtagggta aaataagaaa gtaaataatg agtaggagag
     2761 aatcttatgg aagaagcaaa catccagcga tttcccgtcc tgcgcagtgt atacggcggt
     2821 ctgaccaagt cagagcgccg tattgccgat tatattgcca atcacgccgg cgtcgtcatg
     2881 gaac
//
LOCUS       DAWDXZ010000193         2867 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7244_length_2867_cov_7.657895, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000193 DAWDXZ010000000
VERSION     DAWDXZ010000193.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7244_length_2867_cov_7.657895"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    577..586
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          760 a          765 c          584 g          748 t
ORIGIN      
        1 cttaaaatta aaccgaaggg acgcttcatc aggctgccat tcgattcgtt ccaaccgatc
       61 aagctgagac tgacggcccc tggcctgttt ggccttgata ccggccttgt acttgcggat
      121 atattcttcc gtctcgtgaa tatgttcctg ctgcttctta tacgcccgtt cgagggccat
      181 ttgctgctga tcatgctgtt cgacatagcg cgaataattg cccttgtact tggtcacctt
      241 gccgtgagac agctctaaga tgcccgtggc cacccgatct aggaaatagc ggtcatgcga
      301 gacgatgaga atgccgccgc cataagacaa gagatagcct tcgagccatt ccagcatatc
      361 catatcgagg tggttcgtcg gttcgtcgag gaagagatag tccggccgac gcactaaggc
      421 cttagctaag ttgatgcgcg tcttctggcc gccggaaaaa ctctggaccg gccgatccat
      481 gtcggcttcg gaaaaaccga ggccgcggac aattttacgg gacatcgatt cgtactcata
      541 gccgccgagc cattcaaacc gctcctgcat ccgggcnnnn nnnnnncata gcgattcatc
      601 agcccttcgt cgtcgggacg ggactgcatc tccttttcaa ggccttttaa ttccctttcc
      661 aggcgcatga cgtcctgcca agcctcggta atcagctgcc ccagggtaat atcatcagca
      721 taggtcacat cctgctgcag atagccgatg gactcccctt ccgatacgat aaaggctccg
      781 tcatcgtact cctcttcccc aatcagacac ttgagaaggg tcgacttccc ggccccatta
      841 gccccgatca gaccgatacg ttcgccgcga cgaatttcaa aactgacgtt ttggaagacc
      901 gtgcgcacgc caaaagattt tcccaacttc tgtaccttaa tactgcccat atagcttaac
      961 cttaccctcc tgcaagaact aaaaatcgag tctcatcata agttataatt atatcatgaa
     1021 aaagccgatg cctcaatgaa gggacaaaag gacgacccgg accgcagccc ctccgccgtc
     1081 tcttttccgg caaaacgatg atagccccaa tcatcgtccc tttcgctccc cggccaagcc
     1141 cctgcctcac cgattttcat aaaaaatggt ttatgagcga aaaacgtgct catccgccca
     1201 aaaacgccgc cgaaaaggtt cagcagacac aggagcgatt ccgatgtcac agctcccctt
     1261 agcggcagaa aataaaggac tgcagctgcc ccgcatgaga ggaaacgatt tatcattgcc
     1321 ccctaaatgc cccctcttta tgattttatg ccaggcaaaa aaataaccac gcagcttata
     1381 aactgcgtgg ttatcacatt cattatggtg gagataagga ggatcgaact cctgacctct
     1441 tgcatgccat gcaagcgctc tcccagctga gctatacccc catgtattgg ctacaaaaga
     1501 gattatagca tcaacagccg gtacctgtca agcattttta agctgccgga tcattttcga
     1561 gacgactata tgtattttca gcgaagttct gtacgctttc cgtctcgagc cgttcatcga
     1621 tgcctaaggg aagcttcgcc atgctttcgt cgacatagac ggcgtgcgta ccggcaaccc
     1681 agacgccttg cggcagaagt tcgacggcct ggcgcaggtt ctttgaaata tcgtctgaga
     1741 aaaggtcgcg gtgcatagta gcaaagatgg cgaccttctt tgccttcaag cgcggcaaaa
     1801 cggaccgaga cttttcatca agcctaagat tcaggaacca aacaccgaca aagacaatat
     1861 catagtcatc gaagtgttcc ggagcctgtt ccataggcaa aaaatccgta tccgccggca
     1921 aaacagaacg gaccgcttcg gctaattttt tcgttcgtcc cgtttcggag gagtacaaga
     1981 ctaaagcttt cataatatct atctcctttc gctagaataa gggtcttttt ctatagcttt
     2041 actatacgat aaaaatgaaa aggactccgt tgcatttgca acgaagtcga tttatttcta
     2101 cccctacacg gctaagagaa ccgcaaaaca taccctcagt acatatcgcc atgattgagt
     2161 atcttgttcc taacatcctc tccaaaatag aactaaccac gcggtgatgt accacgtggt
     2221 tagttctatt tcagaaaaac tatatctctc ttgataactg agcttcatat tgtctatagg
     2281 cttcgttata aatggcttcc agatatgatt ccggaaccgg tatttctccc cgactccaat
     2341 accgatcgta tgcatcacgt ccggatatag tccccttatc aaaatcacag atatagctac
     2401 gatgattaga attacctatt aagacataga ccaatgccct atgatttaat ttatcgactt
     2461 caatattatt caaatcaata gaatatttgt cccatttacc ttgcgtgtaa atgaccttcc
     2521 ataaaggctt agcatacagt ggttgtctgc cacaaagtcg tgaaaccata tcagtcagtg
     2581 tatctcccgg aaacagatta aatgtaaccc cctccggagc ttcaaaatcc gtagtctctg
     2641 cattcggtga accattaata tattttgact cgcctatttt tgtaacggtt cgatttttaa
     2701 aatcaatcat ttcatgattc aaatgcgttg ttgtatatat accgtttcga ttcacagagc
     2761 gttcatactt tgtccagtaa gtagcgatat ctcccgcctt atcatatttc acggtctgtg
     2821 tatcaagcga catcttccaa tatgaatctt gatgaagtac cgtccat
//
LOCUS       DAWDXZ010000194         2866 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7245_length_2866_cov_7.505158, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000194 DAWDXZ010000000
VERSION     DAWDXZ010000194.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7245_length_2866_cov_7.505158"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2526..2535
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          759 a          730 c          575 g          792 t
ORIGIN      
        1 cttgagaaat atgaccgatg gcaatgcctc cattttgggt tgcgcttgca ccggtaccaa
       61 tggcaacagc attagtaccc gttgcaccgt cattattgta gttgccctga gtttcatcac
      121 cgtttacgga gaagaaatga accgaacttc caccgctgcc gccaccagtg acagctctaa
      181 gctgcgctac gttgacagca tcggtatcgg cagtacccgc tgctacaccg gtaatctgac
      241 gagtgatgct ctttgatgta tcccctacgg aaacagcacc ggctgtagat ttccagacac
      301 tggccgatga agtggagtta gcttttgtcg agggatcata gccttgctgt cccaaaacgg
      361 ttgccgcaac agagccagta cctaaggcta caccgccggt tgccgtagtc ttactattat
      421 gaccgatagc cacggcatcg gataccgtcg tttcaatttc actgtctgca gtacctaaaa
      481 caatattatt cgacgctccg gtaagcttct gcttattgcc cacaataata ttattcgtac
      541 cattggttac ggtattcaaa gtaccaatca tggtagcata agaggtcgtc gtccccttat
      601 tccgatagcc gttcaacata ttataggaag ctctgctaga ggatgtcccg gtcaaggtgt
      661 taccgacacc ggacaattga gagcgggtag cgtaatccgc tttattgcca ccgccgatag
      721 caagaacagc gccaccaggc gtgccattgc catcagtgcc acggatattg ctgcgcaacg
      781 tatcagccaa atctttcgca tcagaaggtg ccgatccaaa aatatcagcc ggagtctgaa
      841 tattatcgac agagttagta acttcatttc cggcaccaaa aaccaaagta ccattggaat
      901 tattgactct attgactgtc ccaactacag agttagcgat tccggaatac atgttgcttg
      961 atgtttttga ttcaatacta ttgaaagaac cattgataag agcaccaaaa ttctggccta
     1021 ccatcatgac attccccgtt tcataaccac tagtcatgat ggagaaagcc cccgtattgg
     1081 tagtaaaggc cccattggaa taactgttgt gtcccagagt tgttgtgccc aaaacaatgg
     1141 caagatttcg ttttccgctt tccgtcgtag gattcaccgt agtatcacct aaacgaccaa
     1201 tatacttatg gtcaccaatt tgtataccac ttcgagcata cgtattttga ccgatagcaa
     1261 tcccagccat taaatcccta ttatcggtca taccaaagcg gaaggttctt tcctgtccgc
     1321 caaacatatt ttcaacaaag gcatttgtac cgatggccac actgcccttc tgcataacat
     1381 aatcatcaac atgaacatga tcgccgatag ccacggtacc attttgccca tcattattac
     1441 taagtgtgat accgttcccc atcttgatat tcttattcgc cggatcgacg aatgtcgtga
     1501 ccccgtcctg ggcatatacc ccggcaggag cagacaataa cgccaataca gccaaaacag
     1561 cggctgcaga aacacactga cgcgaacctt tcgatacggg tttactgtga ctctttgcta
     1621 tttcagaaac tacgatatag caattcttta ctttgctcca aatgactttg taaatcctat
     1681 tcatatcgct tgcctcctat tagattgata ataaactttc acttaattac ataaaagcag
     1741 acacgatgct gacactttga ttcagcagat ttcttgttat gtcctcactc atcgagcttt
     1801 gatcagacat ataatagcaa tataaaaagc gtaaaatccc ctcgttcatc cctccataaa
     1861 atccgattta tcgcattctt cagctaactg aacttatata gtccaattgt tgcagataac
     1921 gaataatata tatccttatg ttatcattct atatattgtt caaatttttg tcaatgaagt
     1981 tacctgtata taacagtaca aaaacgttat actcgaaatt tagactgttc ttttaccgat
     2041 tatttacgat ttaaccgcaa caaatcgaag tctttcaata gaaaacaaaa ggccctcttt
     2101 atgcaagaga gcccttcgtg tctacgtccg ccagtatgaa cgacgtggcc gcccatgctg
     2161 acgttgttgt tctgaacggt aatgttcttc gacggatctt catttttaac cgtaaggccg
     2221 tcgttgttga tcgtcgtgtt gcccgtcgtg actgaacccg tcacaacaga ttcattggtg
     2281 atgacggtct tgctgtcacc ggtcccgatg gtaacgttgt cgttgctgat ggaaacgttg
     2341 ccaatgttgg ctttttccga aaccgtcagt tccttcgtcg tcatcgtatt cgacgtaacc
     2401 gaattgagat ccttgagatc ttttgccagt ttaacatcaa aaccggtggc gcctgtatta
     2461 ttgacaacgc cgatgttgcc ttcagacaat tcgcccttag caccaccttt aatatccaag
     2521 acgtcnnnnn nnnnngatac aaccgatttc aagctgctga tttcctgagc catagcttgt
     2581 ttcgacgagt aattacccgt ttctccttcg ccaactctcc aggtcacacc ggcgttgacc
     2641 atgttttcac cgccgccata agaagtaccg agactgacca aggtgttgcc attgggacga
     2701 tagaaggcac cgacagctac ggcgttggca ccgcggtaat taccgacacc ggccgaaatt
     2761 tcccactttt catccgcgtt gtattccaag ggatggaggg ctgccaaggc tgcggcaccg
     2821 gcaccgacgc gatcgacgcg agtatccaac ttgctgatgc gattac
//
LOCUS       DAWDXZ010000195         2861 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7256_length_2861_cov_7.494654, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000195 DAWDXZ010000000
VERSION     DAWDXZ010000195.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7256_length_2861_cov_7.494654"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          694 a          804 c          697 g          666 t
ORIGIN      
        1 agcgcgatat ctacttccat ttcaccgtca aaaaggaaat cgacgttgcg gtcacttaaa
       61 atctgaacgg ctttgcgcac cgtatcggcg ccgcggcccc gttcgctccc catcgtcgaa
      121 tacgataaga gggctacctt aggtgaatca atcttcagtg tccgacgtgc ccggtccaca
      181 cagctgcagg caatgtctgc cagttggtat tcatccggtg tcgggatgac gtcaccatct
      241 ccggcaacta aaacaccatc atgaccaaac tgcagcatgt ccgtcagaag aataaaagaa
      301 ctgctgaccg tatcgattcc cttgcgggga ccgataattt gcaaagccgc atgaagcacg
      361 tccggcgccg gcgtttccaa tccggcaacc atgccggccg cctcgccgga agcaaccata
      421 aaggcgccaa aatagagggg cgtccgtaaa aagcgccggc tttcttccat ggtcattccg
      481 gatgctcgcc gcatccgggc atattttttc gcaaaacttt cgaaatgcgt atgttctttc
      541 ggatatatca attcagcccc ctctaagtcc aattgatatc gaaaggccaa ttcctggata
      601 tcctgtcgtt ttccaatcaa taccggaacg gccaatccgt ctgccaaaat ccgatcagca
      661 gcctgcagca tacgaaggtc gccgctttca ggcagaacga ttttttgtgg ttttgaagct
      721 gcccgccgct tcaaagacgt catataccga ttcatacata tcccttccgc atcaagtgta
      781 ttgatttaat tataagaaga gatgccacag aaagcaagaa cgaaagccca tacagccctt
      841 tccgatagcg gagagggctg tatggacttc acgggatagc cttacgttta tttgagaacg
      901 ggctgaacct tgtcttcctt tcccagaagg taatgtttat tgatgtactg cgtgtgaagc
      961 agcgcttcgg acaaatcgct gccgggctct cccaagaatt tcgcatataa tgtctgtagt
     1021 tccttatttt cccagctggc ccgaatcggc tttttggcat ccagctgata gagcgcttca
     1081 atgcgggcac gcttagccgg aatttcaaga ggcaacggag tccgtggctg accgcccccg
     1141 ccaatgcagc cgccggggca ggccatgact tcaattaagg catatttttt ccacgaatgc
     1201 ttttcttcca tcaaattgat gaaccgacgg gcattggcca gtccgctgac ggcaacgaca
     1261 cggatgatgt cgttatcaag agtcagggcc gcttccttga caccttccag tccgcgcacg
     1321 gcttcaaagc ggagaaaatc atcctctgcc ggtttattcg tcttcaagta aacgagagta
     1381 cgaaggaccg cttccatcaa tccgccgctg ttgccaaaga gttccccgcc gccggacgat
     1441 tcgcaaaaga ccgtatcgca ggcagcattt tcaagattgt caaagtcgat tttcttttct
     1501 ttaatccacc cggccaactc ccgcgtcgta atacagtaat ccgtatcgcg catgtccgtc
     1561 tgctgccaat aacggccggc agcattttgt tccggtctgg atatttccgc cttctttgcc
     1621 gtacatggcg tcaccgagac aatgactagt ttggagggat caatgtgcat gcgcttggca
     1681 aaatacgttt taatcatcgg gctgagcata ctgctcggac tccggcccgt cgacaagtga
     1741 gggatcaact cggggaaaaa gatttctgta aattcgaccc atgacggaca cgcactggtg
     1801 aactgcggca acagttcttt cttatgatcc atacgatgca gaagttccga tgcttcttct
     1861 ataatagcca aatcggcacc aaagttagtg tcaaaaacgt aatttccgcc gagccggcgc
     1921 agggccgcta ttattttccc ttccacattg tcgccaaagt ctttcccgaa ggcatcgcca
     1981 agggccacgc cgacggccga cgacgtttga aatacgacga ccttgtccgg atcatcgatc
     2041 gctttttgaa tctcagcccg ctgagaaacg gtcgtcgtcg ctccgaacat gcaggtcagc
     2101 gagcactggc cgcagtggac acagataggg atatcccccg tactttctaa cgagtaatac
     2161 cccgtcatag acatgacttc agcgcagcgg cggcgacaga gggtgcagtt tcggcatttt
     2221 tccggattaa actgaatcga gatattgcgg ttttcaacgg cgactcggct gtcgagttgt
     2281 tcaaatcggc tggtaaaggc catgtctcgt ttcttttcag cctgcatgta aatggcatcg
     2341 ctgcagcaga tagcaatgac atccgtaatg ccttcagccg tacaaccgcg ggaaagatcc
     2401 atgaccggct tggcaagacc ttggaggagc ggccccagcg ccgtggcacc ggacaggtgt
     2461 tccaaaactt tataacaaat attgcccgaa acgagattgg gaaatacaag gacattggct
     2521 tgaccggcaa cgggagaacc gggcgccttc tgacgggcaa tccgcggtac gagagccgca
     2581 tcggcctgca tctcgccgtc aaactcaaaa tcaacgttgc ggtcccgcaa gagacggacg
     2641 gcctcacgga cgcgttctac ttcttcgccg gcaccactgc ccatggtcga atacgacaga
     2701 aaagccactt tcggatcaag ggtttgcgcc gtccgacgtg cccgttctac acaactgacg
     2761 gcaatatcag ccaattgctg cggcgtcggt tcgatgacga cactacagtc gccgatgaca
     2821 accatgccgt catcgccaac ctgaggctta tccgtaatcc t
//
LOCUS       DAWDXZ010000196         2846 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7292_length_2846_cov_7.161591, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000196 DAWDXZ010000000
VERSION     DAWDXZ010000196.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7292_length_2846_cov_7.161591"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1910..1919
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          603 a          724 c          739 g          770 t
ORIGIN      
        1 gattacgatg aattgagtgc cggcatcaaa cagtattttg aagccatcat ggttccgaga
       61 ggcctcggca aatgccgcct ttccttcgaa tgcggccgtg ccatcaccgg tccttacggc
      121 tacttggtca cgacggctat ccatcacaaa catatttgga aggaatatat cggcgtcgat
      181 gccagcatgg ccaacctcat gcgtcccggc atgtacggct cctaccacta cctgtcggtc
      241 atgggcaagg aagacgctcc caaggatcac gtctatgacg tcgtcggctc gctctgcgaa
      301 aactgcgata aattcgccgt cgaccgcagc ctgccggaaa tcgaagacgg cgacatcctg
      361 gtcatccatg acacgggggc tcacggccac tccatgggct ttaattacaa cggcaagctt
      421 cgctcggccg aactcctcct tcatgccgat ggaacggtga ctcagatccg ccgggcggaa
      481 acgttagatg acctctttgc gactatcgac ttttcaaact tataaatgaa agtaaatcat
      541 cctccttaca tactgtaagg gggatttttt ttcaatttat tttaaatttt aatatatttt
      601 taatctttat cgagtataat acagatataa cataccgtca ttcatctgaa tagggagaaa
      661 ggaggcatct tggctgatgg gggcttatga tatattgacc gggacccagc tttgcatggc
      721 tgtggccatg attgcctatg gcttttactt taaaaagaaa cgaacgtgcc gcgtgtggta
      781 tttctccggt ccggcatggc tgattgccat ttggtcggct gttgtattat actactattc
      841 agaatatcct tttttaacga tcctctgctt cctcatggga tttttcttag gcatgggaat
      901 cggattgtgg gttaaaaaag gcctgccgga actgacctac tgcagggaac ggcggcgcct
      961 ccaatgcccg ccgatgaaga aggccatgcc ggcctatctg atcatctgca tcgtatttgc
     1021 ctccttccag ttcgtgggct accatttttc tttcattacg cactcctggt tctttaatga
     1081 agtactgggc tttgcaccgg gactctgtac gggtttgttg tggggaaggg gcattgccat
     1141 gctgctcgat atccctgtac ggggacagca ggtatataaa gatatttagg agggaatttt
     1201 catggacaat acacagcgga gagaacaaat ccttcagatg ctgcacgaat cgacggctgc
     1261 cgttaccggt acggccttag ctaaggcctg cagcgtcagc cgccaaatca tcgtcggcga
     1321 cgtcgccctt ttgcgtgccc aaggggtcgg catcatttca acgccccggg gctatcagct
     1381 ggttacaccg ttaaagaagg gctgtacgcg ggtcttcgtc tgctgccacg gtatggattt
     1441 gatggaagcc gagctcgaag ccatcgttga caacgggggc atcgtccaca acgtcgtcgt
     1501 cgaacacgag gtctacggca atttggaagg gaccttgaat cttcattctc gccgcgacgt
     1561 ccagcagtac atcaagcgga tgaaagactc taaggccgaa ttgctgagca ccatcagcgg
     1621 cggcattcat acgcatttgg tcgaagccaa gacggaagaa gaactcgatg ctattcataa
     1681 ggctttgcat gacttaaagg tcttatacgg cgattgactt tttccttcca tatgctacaa
     1741 tgatgtcatg tcaaattatg tgtcatgaca gttgtacaga taggaggaaa catctcatgt
     1801 tgttagaccg gttggaagcc ctgcctgtag gcaggttcca ctataaacta ctcttggtca
     1861 ccggtttggg ctggcttttt gattccatgg atacagggct catcgccttn nnnnnnnnnt
     1921 gctccgtcgc gggtgcgcgg ccggttcatc gtcctcttgg aaagcttttg gggcctcggc
     1981 tggattgccg ctgcctgcat cgcctatttc ttcattcccg tctatggctg gcgcatggct
     2041 ttcttgatcg gtgccctgcc ggcgctgtat gtctgcctca ttcgccttca tatgccggaa
     2101 tcggtccgtt atctcctggc tcacggccgc gtagcggaag cgcggaatat cgtcgtttcc
     2161 atcgaaaaaa cgctgcatgt gcccgtcgca cctttttccg atgccgatga acggatgccg
     2221 actgtaacca aggcttcgtt ccatgaattg tggaaaaagc cctttatctc acggacggtc
     2281 atgctgtggc tggtttggtt cggcattaac ttcagctact acggtatttt catgtggttg
     2341 ccgtccctcg ttttccagca gggattcacg gtcgtcaaga cctttgaata cgtcccgcct
     2401 ggctggtcga caaaatcggg cgcaagtata ccttgtcgtc gttcctctta ttcagcggcg
     2461 ttgccagcta tttcttcggc cacgcttcga cggcttcgat gctcatgatc tggggcgctg
     2521 tcatgtcctt ctttaacctc ggtgcctggg gcgtgctcta tacctatacg ccggaacagt
     2581 atccgacggc cattcgggct ctcggaagcg gctgggctgc cgggtttgga cgctttggcg
     2641 gcatggcggc cccggtcatg gtcggtgtgc tcttggggca gcactttgat ttcggtggtg
     2701 tcttctatat gtttgccttc gtcttcatcg ccattgccgt cgtcgtcatg ggcctgggca
     2761 tggaaagcaa gcaaaagaac ttagagagca tgactgaaca atttgccgat gccaagtgat
     2821 gtaaattttg taaaaaaggg ttaatt
//
LOCUS       DAWDXZ010000197         2839 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7305_length_2839_cov_11.696839, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000197 DAWDXZ010000000
VERSION     DAWDXZ010000197.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7305_length_2839_cov_11.696839"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          595 a          780 c          837 g          627 t
ORIGIN      
        1 ggttccccga tgaccagcac gtcacaaggc gtatcgtcgt aagcttgttc cgtcttccag
       61 gcattgaggt cggccaaggc gtactgagcc ttgacgtatt cttcgtctcc cgtaaggccg
      121 gtcacataag aagaatcgcc tttccgcagg gcttcgagcc aatcggccgt ccccttttcg
      181 cccatgggaa tgccgacgac gtagggaatc ccgtacgtat cgcgaaggta ctgggccagg
      241 taaaaggccg tcgacgaaac gacggcgttg acgtcggcct cagcagccgt ggccaggccg
      301 tcgagggtgt cgcccatgga ccacgagctc tggatggtaa atccggcgtc agtcacgatc
      361 tgtttcagcg tcgtagcatt gccgacgacg gaaaaatcga ggggcgtcaa gcccaagagg
      421 ttgacccgtt tggccggaag ctcgggcttt ttcgaagccg gtttgacaaa ctgcttggcc
      481 agccaaacat aggctccgcc ggcaccgggc agataactgt ggatgccgtc cgtgcgaaag
      541 cctaaggtcg gaaggccggt ccgatcggct accagtcgct gaatggcctt aaagtcggta
      601 ccgatagcgt ttggcatggg gctgccgccg atggcgatga aataaggttg tgccgcttcg
      661 gcagcttcgg caatgtcgtc gatgagccgc tggtcatttc cttgaatggt atcgatttca
      721 ttgaggccgg aaatatagac catgctctgc gtatcgtagg accgcggttc atcgtgcgta
      781 tcgtacgtcg agttgcagcc cgatgcatca tgcatgacga tgaggccccc taattcatag
      841 agcgacgacg cgatgccgaa catgtcgacg gcatacgtcg ataagactcg ctgtgcttgt
      901 ttcaaccgtg acacccccag gcttttatct gtatgatttc ccgcatcttg ggattttctg
      961 cggcattggc ttcaccgatc cagtcgagga ttttcgacag gccgacgtag ccgtaaaggc
     1021 cgctgttttc gatgaggttg acgaaatact tcgtatcggt aaaataggcc gccatctgac
     1081 cgatagccaa gaccttgccg tacttttgac tgtcgttgcg cggcagaagg ccccgcttaa
     1141 agtgcatggc cgaccgcagt tcgaggtcgg gatattcctt ctgcaagaac tcaaaggcct
     1201 ccttttcccc cggtaaaatc ttatcgaggt agacagcata aacatggaag ccgtgggtca
     1261 agaggaactt ggccagcccc aagggccgcg gcgtcgccgt atagtcgata gcaatgggcg
     1321 tcgagcccac ttcttcgagg agggccttag ccttggcttc gatttcctgt tcataagcgt
     1381 cgcagtcggg catggcaagg ccgaaggcgt cggctgcggt ctgcaaattc tgccgcagga
     1441 cggcgtagtc ataagaatac gtcagataga gggcttcttg tttcaagcgc cgcttcatgt
     1501 ccttggccgc cggctcggag ttttccagaa aataaatgtt agcttccgat tcgcccatgg
     1561 ccttatactg ctcgtacgtc ttgcagtcgg caaaatcgtg gatgtcgtaa ccgccgtcgc
     1621 gaagcatctt cgataattca ttatcctcat cgacgggata ataattgacg acgtaattga
     1681 cggccttctt tcgcggcaag ggctgcacgg cccgcatgag ctgacggcga ccgaggacgt
     1741 ccggcgaata gcggcgctgc agggtcggta tcatataacc gtcgatgaag tcgatattgg
     1801 ggtagcgctc gcgaagggtc ccgtaaacga cgttgaggtc gatgtgcagg aaatgctgca
     1861 tgcagctcgt aaagccttcg acgcacttcg gccgctccgg cagttcgtcg atgatatggg
     1921 caatgccgtc gatcatcatt tcttctaagt tgcctttaat caaattttca ttggtcaggg
     1981 taaccatgga gaagcggccg gccccttcag gcacttcatc ggccgacaag acgacgccgt
     2041 ggaggcagca ggcacagcag acgtagattt catggcagcc gggaatgagc atcggcgaat
     2101 gagcaatcgt ccaatggcct cgggccggcg aagcgtactg caggccgata tagaagggct
     2161 tggggaagga cgccttgcca atggcaatat ttgccccatc gggcgttaca tggttatacc
     2221 gccgcgcctg gggcggattc gcttcatttt cgtatatcat tcccgtacct ccatcttaac
     2281 aaatgcgggg cagcaattca ccgcccggcg gcggcagcag cgtttcggca ccgatttctg
     2341 tgcgcatgac gacgcggccc ggctggtcgg ccgtaacttc accgataatg gctgcatttt
     2401 gcgaatattt tcccttacgc aggacggcga cgagatcgtc ggccacatcc cgcggggcga
     2461 agatgaccaa acggccttcg caggccagat acaacggttc caggcccaac atgccgcaga
     2521 cgccgcgtac agccggatcg acaggaatgg cctggctgtc caaatgaatg ccgaccttac
     2581 tttgggcggc gatttcatag aggacggtgc cgacgccgcc gcgggttgca tcgcggataa
     2641 cgtggatatc cttggtcgtg ttgagcatgc tttcgaccgt tccccataaa ggtgcgcagt
     2701 cactggtcac atcggcatcg atgccgaact ggttccgttc gaggagaatc gtgcagccgt
     2761 ggcggccgac gtcgccgctg acgatgatgg cgtcgcccgg tttggccaag gtgcctgaag
     2821 tttggacacc gtccatgat
//
LOCUS       DAWDXZ010000198         2837 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7318_length_2837_cov_8.676132, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000198 DAWDXZ010000000
VERSION     DAWDXZ010000198.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7318_length_2837_cov_8.676132"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1342..1351
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          613 a          766 c          758 g          690 t
ORIGIN      
        1 cggtgtccgg taaaaagata ccgcttgcag ttttttcttc ttgttccaat acgcggataa
       61 cgacgcgatc tcctaacggt tttaacattg tatattcctc cttagacgta agttgctagt
      121 ctttatcagc actcttcact tctgagtgct aacttacaat atatatatta gccattaaag
      181 gtgaaaaagt caaggcttgg gcctcaactt tttctttaac ggctttattt tttttgacct
      241 tttatctttt ccgctccggc aatgaccgat tcggccactg ttttcaaggg tttacggttc
      301 ttcatgctgt attgctggag gcgcctatag gcttcgtctt ctgaaatatg atataagtcc
      361 atgagacatc ccttggcccg atcgacggcc ttgcgcgttt cgatccggtc gttcatctct
      421 tccagctgct tggctgcttc ccgctggcgg ctgaattgag ccatggcgat ttcaatggcc
      481 ggaaagaggt ttaccgggct caccggtttt accaggtagc cgagaacgcc cgagtctttc
      541 gctttatcga ccatgtcctc ctggctgtat gccgtaagga gcagcaccgg cgccaatcct
      601 tcatgaccga tcatgcgggc agcgtggatg ccgtccaggc gcggcatctt gatatctaat
      661 aagacgatat ccggcttttc ccggcgcacc aattccaggg cctccagtcc gtctgccgct
      721 tcaccgacga cggtgtggcc ggcatcttcc agcatttcac ataaatccat tcgaataatc
      781 gattcatcgt cccctattac aatgcgatac cccatgtttt agtctcccct ctttgacaat
      841 ggaaagcgaa tgaccgccgc cgtcccctgc ggctgcgcct tagaaaaggc aatcgttccg
      901 tgcagatcct tttcgatcag cgtttgcacg atggccatgc cgagatgttt tcgcgtcgtt
      961 tccttttcgg tctggtccat gccgacgccg tcgtcacaga cggtaatgac gcaagattct
     1021 ccttcacagc gaatcgcaat ggaaatcacg ccgtccgacc ggccttcaaa gccgtggatg
     1081 atggcattgg taatgagttc attgaggatc agggccaaag acgtgacgac atcggaagaa
     1141 aggagcagct tttggccgga aaacttcgtt tcgacgcggc attccttact gaccaagctg
     1201 tgggccagga ggttgagcaa tttttgagcg acgtcggaca aattgatgtt ttcatcgtcg
     1261 tcataggaca gggtttcatg gaccagagca atgctcaaga tccgattcag gctctccttt
     1321 aagacgtccc gtgcttcgtc gnnnnnnnnn ngatggaaat gacgcgccgc accttgccgc
     1381 gctgcaaaat gggaatgacc cgccgcgaca cgacggcatc gccgacagtg atatcgtccg
     1441 ccaggccgtt ggccgtggcc aagacctgtt tgccgccggt cacatcgagg ctggtattgt
     1501 aaatattggt ccccgtaatg atcccgtgct gaccgcacat gtggaggata ctgtcggcca
     1561 tgtcgtcggc ataaatgacg atgccgttgc agttgaccag gacgacgccg tcctgaatcg
     1621 ataagggccg gtacatggaa tgaaaatcca tcggcacttg gagggagaga aacgccgttt
     1681 ccgtcaataa ctcccggtta tcctcgacga cgccgacaaa ggcgatgacg gcaatggtcc
     1741 tgccgccgtt atcgacaaag ggataagcca caatgggcgc tgtacggccg tattcgattt
     1801 ccttttcccc atgcatggcc ttgccgtctt gaaagacctg atagttgaaa ggttcttcat
     1861 aatgagaaaa gaccgtcccc ggttcggcca gttcattttg ccgccccttc tgcaacagcg
     1921 gcgtccgatg agcggccagg accagcgtcc ccttacgccg tccgggcacg tagacgaaga
     1981 gctcccggtg cgacaagtcg ctggcaaact gcagcagctc ttcactattt tgtaagatat
     2041 ggatctgaac cggcgacagc gtgctggctg tccggcaaat gggtgcaagt gcagtcacgg
     2101 aaatcccccc agtacggcct caaagattat tccccggtaa tcggcagggc cggtttcgca
     2161 ttgagtgtca aatcggcaaa gcagccggcc ttagccatat agtaagcccg acagccaatc
     2221 atggctccgt tatcggtgca aaggatcgga tcgggatggt aaaaggcata gcccttttta
     2281 tcgcaggcct cctgcatggc cgccgacagg cagccattgg cagagacgcc gccggccagg
     2341 acgatttttt tggcccggct gtacgaagca gcatccatcg tcttttccac caaggtttcg
     2401 acgacggtct tttggaagct ggcggcgacg tcagcggcat tataggactc gccccgctgt
     2461 tcctgggtat gaagatagtt gaggacggcc gatttcaagc cgctgaagct gaattcaaag
     2521 ttatgccgtt catgaagggc tttcggaaac tcaatggcat caggattgcc gtctttggcc
     2581 agcttttcaa tctgcgggcc gccgggatag ggatagccca tgacccgggc aatcttgtcg
     2641 aaggcttcgc cggccgcatc gtcgcgggtc tggcccatga gcttgaagga gttatagtca
     2701 ttgatctgga cgagctgcgt atggccgccg gaaacgacga gggccaaaaa gggcggttcc
     2761 agttcgggat ggctgaggaa gttggcaaaa atatggcctt ccatgtggtt gacgccgacc
     2821 aagggcttat cggcagc
//
LOCUS       DAWDXZ010000199         2821 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7360_length_2821_cov_7.722343, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000199 DAWDXZ010000000
VERSION     DAWDXZ010000199.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7360_length_2821_cov_7.722343"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          607 a          793 c          805 g          616 t
ORIGIN      
        1 ctgcagggga cggtagaagc tccgtcttcg aagagcatcg gtcaccgcga cctcatttgc
       61 gcagccctgg cagacggcga gagtctcgtt gacaatatct ccttatcaca ggatatcgag
      121 gcgacttgcc acatcctgaa ggccttaggt gccgatatta caccggtggc ttcggttcat
      181 cccggaagat cggcttacct cgtcaaaggt gggctacgga aacagtccgg gactttatcg
      241 gtcgatgcca atgagtcggg gtcgacactg cgattcctcg ttcccgtcgc cgtggtaagc
      301 ggcaaccgct gcaccttttc cggaagaggc cggctggcaa aaaggccatt gactccgtat
      361 tatcaactct ttgatgaaaa aggcctcgcc tatgatacga cagacggcgg cctgcctttg
      421 acggtagaag gccgtcttac agccggatcc tacgcgctgc gaggggatgt gagctctcag
      481 ttctttacgg gactgctcat gaccttgccc cttctcgacg gtgattcgca ccttcgcagt
      541 acgacgcccc ttgaatcggc aagttatgtc gacatgacca tcgactgcct gcgccagcac
      601 ggcgtcgtca tcgataaaga atgggacggt tcttaccgca ttgccggcaa ccagtcttat
      661 cagccgggga catatactgt cgaaggggac tactcccagg cagccttttg gctctctgcc
      721 gggaccttgg gacgggccgt ctgctgcacc ggccttcgtc agaactcctc tcagggagac
      781 gaagtcatcg tatccctcat ccgcgacatg ggcggccaaa ttgaaggtaa gacggaattg
      841 acggcctgcc cgtcggacct tgtcggtcga gtcatcgacg tgaaggactg cccggatctg
      901 gtgccggtcc tgactgtatt ggcctcttgt gccaaaggga ttacccgcat catccacgcc
      961 ggtcgggtac gcctgaaaga atgcgatcgc ctgcacgcca tggcgacgga gctcaataaa
     1021 atcggcggcg acgtcgaaga agaagcagaa ggactcatta ttcacggcgt cgacggcttt
     1081 acgggcggta cagtcagtgg ttggaatgat caccgcatcg ccatggccct ggccgtcgcc
     1141 tcgcagcgct gcagccggcc ccttcggatt gaaggtgctg actgcgtccg taaatcctat
     1201 cccgacttct ggcaggattt cgagacctta ggcggtatca ttgaaaagga ggaaggataa
     1261 gatgaatacc tttggacaga ccatcggcct tacgattttc ggggaatctc acggcgcttc
     1321 catcggcgcc gtactggacg gccttccggc cggaatggcc attgactggg aggccgtcag
     1381 gcaggaaatg gccaggcggg caccgggaaa gaataagctg tcgacgacgc ggtcggaaaa
     1441 agacgccttt gaaatccaaa gcggattttt taacggccat acgacgggga cgcctttatg
     1501 tgccatgatt cgcaacggtg atcagcattc gcgggattac gatcagctgc gtcatatcat
     1561 gcggcccggc catgccgatt actccggtaa gattcgctat ggcggcttca acgactaccg
     1621 cggcggcggc catttttccg gccggctgac ggcgccgttg gtctttgccg gtgccgtcgc
     1681 ttcccagatg ttggccagta aaggtgtcgt catcggatcc catatcctgc gcgtggctga
     1741 tgtcacggat gcgagctttt cgcccctcgg tgaatcggca gaaactttgc aggccttaaa
     1801 agcggaaacc cttcccgtcc tcgataagaa aaaggccgcg gcgatggaag aacgaatcgt
     1861 agccgctaaa aatgacctcg actccgtcgg cggcgtcatc gaatgtatgg ttaccggcct
     1921 tccggcgggt ctgggcgatc cctttttcga ctccgttgaa agctgtctca gccatatgat
     1981 gttttctgtt ccggctgtaa agggcattga gtttggcgac ggcttttctt taaccggcct
     2041 tcggggatct caggccaacg atgctttcta ttacgaccaa gaaggtgtgg tgaagaccaa
     2101 aaccaatcac aacggcggca tcaacggcgg cattaccaac ggcatgcccc tcgtattccg
     2161 cctggccgtc aagccgacat cgtcgattag ccgggcccag gacacgattg atacggatac
     2221 gcaggaaaac tgccaactgg tcatcaaagg gcgtcacgat ccatgtatcg tccaacgggc
     2281 cgtgcccgtc gtcgaagcgg cagctgcttg ggccatcctc gacgtttggc tgtcgacgaa
     2341 agggcggtgt ctgtaatgga ggaacctggc gagaaagaac tgcgcatcgc ctgtttcggc
     2401 cggcccggtt cctatacgga tgaagccatg gggctctatt tcgacgatcg ggattttacg
     2461 gcggcgtatg tcccgcattt tgaagacgtc atccaagccg tcgcttcccg gaaggtccga
     2521 tacggcgtct tgcccattga aaattcctcg accggcggaa tcaccgatgt ctatgacttc
     2581 atccatcgct atgactgttg tatcgtcgga gagaaatacg tcaagataga ccattgcctg
     2641 ctcggcctgc cggggacaag gattgacgac cttcgtgaag tctattccca tattcagggc
     2701 ctgaatcagt gccgggacta tctgaaacat cacccaaagt ggcagctcca cccttatttc
     2761 agcacatcag agagcgccga aaaggtacgg gatatgaaaa atccctatat cggggccatt
     2821 g
//
LOCUS       DAWDXZ010000200         2810 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7384_length_2810_cov_9.302359, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000200 DAWDXZ010000000
VERSION     DAWDXZ010000200.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7384_length_2810_cov_9.302359"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    312..491
                     /estimated_length=180
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1737..1746
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          682 a          650 c          654 g          634 t
ORIGIN      
        1 ataaggagca caagttttac ttagtccgcg aagatatttt gcctgaagcg atcaagaaga
       61 ccatcaaggt caaggaaatc ctcaagcgcc atcagacgcg gaccatcaat gaagccgtcc
      121 agatgatgga tttgagccgc agtgcgtact ataaatataa ggattacgtc tctccttttt
      181 atgacgttac gcagggcaaa atcgtcacct tgtcggtcat gatttttgac cgtcccggcg
      241 aattatccca ggttctcaac acggttgctg ccaacaatgg cagtatcctg accatcaatc
      301 agggcattcc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn natatgaccg tatcggtcga agacttgatg aaaacgttgg aaaaactgtc
      541 cggtgtcagc aaggtaaata ttatcggaca agcgtaagta aaaaagaggg ggtaggtcca
      601 tgaaaagcgt ttctattgct ctattaggct gcggtactgt tgggaaaggc gtcgtcgatt
      661 tattggcgat gaacggtccg cttattgaag aacagctcga tacgaaaatc aatatcaagc
      721 gtatccttat ccgtgatttg aacaagtatg aaaagatgga aggcctgccc gattccattc
      781 ggctcacgac tgattttaac gacatcatca atgatgatga aatcgaaatc gtcgttgaag
      841 tcatgggcag cgccgacttt gcaaaagact gcatcgaaca gtgcttccgg cgcggaaaga
      901 gcgttatcag cgccaataaa gacctcatcg cagactatgg gattccttta ttgaccttat
      961 cctatgagca tgacgtcgac ttccagtttg aagccagcgt cgccggcggt atccctatcg
     1021 ttcgtcccat gtattcatcg cttaacagca accgcatcga agaaatcatt ggcatcatga
     1081 acgggacgac caattttatt ttatcgaata tgaccgccca tggttcgtca tatgaagaag
     1141 tattgaaaga agctcaggaa aaaggctatg ccgaagccga cccgaccaat gacgtcagcg
     1201 gctatgatgc agctcgtaaa atcgctattt tggcaacctt aggcttccat tcagccgtta
     1261 cctttgacga cgttgccgtt gaaggcattg aaaatatctc caaggaagat attgaacatg
     1321 cgacggaaat gggatacgtc atcaagcttc tggccattgc ccgtcaggac gacgacggta
     1381 tttcactcaa cgtccatccg gcctttattc gtgaagacca tcccttggcc catgtcgacg
     1441 gtgcctataa tgccgtttgc gtccgcggca acggcgttgg caacgtcatg ttctacggcc
     1501 cgggtgccgg atcgcttccg acggccagtg ctgtcgtcag cgacgtcatg aacgtcatcc
     1561 tccaccgcaa tttgaagatg accggtaagc gcggttatgt atggcacgaa gcgaaattga
     1621 agcctcaggc tgaaatttgc tcgccctact atctgcgaat gatcgtcaac aatgcgcctg
     1681 gtgtcttgtc gtcggtatca aaggtcttgg ctgaacacaa tatcagtatc cgctctnnnn
     1741 nnnnnnaaac gacagaaatt gctgaaatcg tcatccttat cgacaaatcg gccgatggtc
     1801 tggtccaggc atcgctccag aaaatcgaag gattgtcatg cgttcgcgaa attgaaaatg
     1861 ccattcgtgt aattgaggtg taagaaatga caaaaacatt gcatgcacag attcctgcaa
     1921 catccgctaa cgtcggctcc ggtttcgatg ccgtcggtat tgcgctgaac ttgtacaatg
     1981 acatttattt tacggaagaa ccgggcaaag acacgattac cgtggaaatt gaaggactcg
     2041 gagccaacaa gctgcccaag gtttttgacg aaaatatggt cggccatgct atgaaggccg
     2101 tcgccgtcaa gaagcgtcag tcccttccta agggcggcac gctgaaactg gtcaatgcca
     2161 ttccgccggc ccgcggtctc ggaagcagct cggcagccct tgtcggcggt atcctcatcg
     2221 gtgacgcctt gaccggcagt accatgagcc gcgacgaaat gctgtccctg gcaacggtcc
     2281 tcgaaggtca tccggacaat gtggcgcctg ccatttacgg cggcttatcc gtttctgtca
     2341 tggtcgacgg caaaacgctg accaattcaa ttccccttga tgatgaattg tcttttgtga
     2401 cggtcagccc ggaaatcgaa atttctacag aagaagcgag aaaagccctg cccaagacga
     2461 ttgattacca aaacgccgtc tttaacgtca gtcgcgtcag cttcctggta tcgtctttat
     2521 ttacgaagca atacgaccgc atcgcttatg gcctgcagga taagctccac gtaccttatc
     2581 ggttgagcct cattcccagc ggtgatctgg tcttgaagaa ggctgtggaa gccggtgccc
     2641 ttggggctac gatcagcggt tccggttcga cgctcatcgc ttttgctacg ggcaatgaag
     2701 gagccatcat ggaatccatg atcaattgct ttaacgacca cggcattgag gcaacgggtc
     2761 acgtgttgaa atgcagcaat gaaggggcga agctcgtctg atgattcaag
//
LOCUS       DAWDXZ010000201         2800 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7405_length_2800_cov_8.347905, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000201 DAWDXZ010000000
VERSION     DAWDXZ010000201.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7405_length_2800_cov_8.347905"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1439..1448
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          536 a          736 c          802 g          716 t
ORIGIN      
        1 ataagcctga tgagtttctt cctggtgcgg gcctggcggg acgatacgta tcggttggcg
       61 atggaaaaag ccgtcctccg tgttcccggt gttcagtcct tctttttggc gtgggcctgg
      121 cagcgcttca gccgcatctt agccgtccag ttgtccggtg gaattccctt attggaatgt
      181 ctgtccggcg ccggctccgt cgtaccgtcg ctctcgtttc aaaactacgt ccgctgcctc
      241 cgttttcggt tggaacgggg acagaccttc agccaggctg ttcacgacag tccttatggc
      301 acggcctata tcgagaccat gctcaccgtc ggtgaaatga ccgggaaata tgacgatgcc
      361 ttaacggcca ttgccgttta ttatgacagc cgccttcgtc ggtgggcggt cggtctgcag
      421 cgctggctgg ggccctttgt cctgatcatt gtcggcgtct ttatgggatt tttgatgttt
      481 tcgctgctct tgccgctgct cgatatggca tcttccgtcg ttacttcttg aaaggagtct
      541 tatcatgact atgcgaaatg gtttttctat gttagaaatg ttaatctgtg tctctatcat
      601 cctggtcttt gcatcctttg ccgtaccgaa gttcacatcg gcaacgaaga cggcacagga
      661 agccaaggta caggccgata tccgcactat cagcaatgct gccgcccttt atgaaatcga
      721 ccacggtgaa ttcccgacga ctgtcgatga gttggtcaag gtggacggcc aaggacggca
      781 gtacctgcaa tttaagccgc tgacgccgga taaagaagag taccgcatcg aaaatggcgt
      841 cgtttctgcc gattataacg ataagcatta ttcctctgat tccgacgcag gcgtgccggc
      901 cggaacctaa gccatggagc tctttggcct ttggtacggc ttggcttttg gggctacggc
      961 cgccattacc tataccgacg tcaaagccta ttggattccc gatgctcccg ttgtcgtcct
     1021 ggcagtcggc aatatcctgt ccttgggcct tggactcgtg cgaccatcgg tcatggccgt
     1081 cagtgcgacg attttctttt ttctcctcct ctacgtcctc tggccgaaca gtatcggcag
     1141 cggtgacatt aaactggcct tagccctctc tatcggctgt ccggcgggag gggcctggct
     1201 gatgaccgtg acggctttct tgtccgcgac ggtcatctcc cttctctgtt ggagcctaaa
     1261 ggggaagacc atccttccct ttgggccctt cctcctcttt ggctggtggc tttcacagga
     1321 gattcacttt cagtggttcg gtatcggtct cggcttatga aacgacgcaa tggatttatt
     1381 caactgacag gcctgttgac agctgtgttc ctcctcggcc tttatttggc cttggctcnn
     1441 nnnnnnnnca gtccctcagc cgtcatcaag cccttgacgg cctcgtgcgg gagttggtct
     1501 ttgatatgga aatcgcccgc cagcacagcc ttggcaacaa cgtcggcaaa gattactgta
     1561 aattgatgct gcaagaggat cggtatgtct tgcagcatcg ctatcgagtg aagaaaaaaa
     1621 ggccttaccc gccggggata cgggtgacga aaggagtggt gcagtttgat gtttacggcc
     1681 gtccgaagta taaaatggtg gtcgtcgttt cggcagacga tgagcgctat gagcggcgca
     1741 tcgtcgtggc ggctcagacc gggcgcatcc gcatcgagta ggaagaacgg ctttttcttc
     1801 ctggctgacg gcatggcagc cttttttatc ggagccgtcc tgctgcttgc cgccggagcc
     1861 cttgccgtcc aagccctgga gctgcaccgg cgggctgcct ctcgctatca ggcggtcatg
     1921 gtcggccgtc aggccatgga acggtggaag gccggactgc ctttgccgga tgagatagaa
     1981 gtggccggac atcgttatgg aatccggcat cggcagtatg gcgagcaacg agggtatacg
     2041 gtatgggaag tcgaggtgga cgatgaaggt ggcaaaccgt ttttctgtcg gcgcctgggg
     2101 ccgattccgc cgaaggaacg gctggatgat gatggatatg ctgctggcca tgaccttcgt
     2161 cgtattggcc gtgacagcct tcctgcccct gatgagtcag accgtgagct ttgatagaag
     2221 ggcccatatt caagaggcct tactgcgtca ggccgtgagc attgaagcga ccttgttcca
     2281 agaattggtc tacggtcagg aatgggacgt gtctttcgac agtattcgct ttacaacacc
     2341 gcaggggagg cgcaaaggct ttctggtgca cggggacgtc ctctttgtcc gcctcaatga
     2401 cgggacgtat cagccactga ccggcggtgt cggtacggtc aagggctgtc ggattctgat
     2461 ttacccttac gacaaaaggc ccttcttttc tcaagaaggg caggccgttg ccgtatccct
     2521 tcttctcgta gaggaagaaa gcgggctgtc cctgccgatt acgcttatgg taacgtcgtt
     2581 gaatgaagga aggcgatgaa gatgcgaaac ggattctttt ctttttcgat gacggccatg
     2641 gccgtcctga ccttgtccct ggccatgacg acggctttgt tggccgtgag cagcctgcag
     2701 atggttgcca tatatgaaaa gaaagtccgc ctgcactact tggcggacag tgccgtccga
     2761 gaaggatggc aggaggtaca acaaaatcca cttccttatt
//
LOCUS       DAWDXZ010000202         2799 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7407_length_2799_cov_8.733965, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000202 DAWDXZ010000000
VERSION     DAWDXZ010000202.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7407_length_2799_cov_8.733965"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          562 a          743 c          824 g          670 t
ORIGIN      
        1 tgcccctggt gttgccgccg acggtctgcg gctggctgct gctcttggtc tttggacttc
       61 agcatcccgt cggcatcttt ctcagtgagg ccggcatccg cttcgtcatg acctggtacg
      121 gcggcgtcct ggcatcggct gtcgtcgcct ttcccctcat gtaccgcacg gcgcgggggg
      181 cttttgaaag cttcgatgaa acactggcct ggagtggaaa gaccttgggg ttgtcggaca
      241 cctttatttt ctggcatatc cgcatgccct actgcaaaca gggcatcatg gccggcacgg
      301 tcctggcctt tgccagagct ctcggcgaat atggggcgac gagcatgctt atcggctata
      361 tccccaagca tacggcgacc atttcgacga cggtgtacca attatggcgg accaatgatg
      421 agtacagtgc cttcatttgg gtcttggtca acatcgccat cagtgccgtc gtcttgatga
      481 cggtcaacct ctttgaacag cgtaaccgtg aaaggaggcg cccggcgtga ttgatatttc
      541 ggtctataaa aactgggggt cctttaccct cgatgtagcc cttcatcatc aaggcggtat
      601 ccttggaatt ctcggggctt ccggctgtgg gaagagcaaa acactgcagt gcgtggccgg
      661 cattgaaacg cctgatgaag gacacatctc cgtcggaggg aggatcttct ttgatcgtaa
      721 acgaggcatc gacgtcccgg ttgcccggcg gcgggtaggg tatctttttc agcattatgc
      781 cttatttccc aacatgactg tcgaagaaaa tattcgctgc ggcgtccgct cgtcgacaag
      841 ggacctggct tggaagcagg tgaagtccat gatggagcgc atgcgcctta cgggccttga
      901 agggcagaag ccggcagagc tgtccggtgg tcagcagcag cgggtcgctt tggcaagaat
      961 tctggtaaat gaaccggata ttctcctatt ggatgaacct ttcagtgcct tggacagtta
     1021 tttaaaggaa aaagtcatta cggaactgaa ggctctcttg gcagaatttc ccaaggatgt
     1081 tatcttagtt acccatagcc gcgatgaggc ctatcagctc tgccattcca ttgccgtcct
     1141 cgatggcggc cgggtgtcgg ctgtcgggcc gacaaaggaa ctctttgcca atccccagtc
     1201 cagggcggca gctgttttga cgggctgtaa gaatatcgtc tcggccagaa aaagcgggtc
     1261 ggaatcggta ttcatccccg attggcaagt cacccttcag acggaaggac gacctgtcgg
     1321 ggacgatctc tgcgctatcg gaatcagagc gcacgcattt cacggcggcg aaggagcaaa
     1381 ttccctgccc atcgtcatta cggaagagat tgaacagcct tttgaatgga tcgttaaatt
     1441 ccgttatccc cgtcagtctc tggcgacgcc cgatatttgg tggcgctttg ccaaaccgga
     1501 tcggcccgat atcctgccgg accgcttaac acttaggggt gacgatattt tactacttta
     1561 caggtgatga ttatggaacg gatatataca gcattatatg aaaatctaaa ggccggccgc
     1621 ccggctgttc tcctgactgt tgttgagcga aaaggttcga cgccgcgcgg tatcggaagc
     1681 catcaggtcg tctttgttga tggaacgacg atggggacgg tcggcggcgg ctatcaggag
     1741 cacttggcca ttgaatgcgg caaagcctgt ttggctgaaa aacggtctga ctttcgccgt
     1801 cttattcttc atcccaatga cgtggaagac atcgatgctg cctgcggcgg cgaattgacg
     1861 gtattttgcc agtacttcga cccggctttg ccgaaggtcc ttgaggtact ggcagcgatg
     1921 atctcagcct tacagcagaa gaccgcttcg tggctggtct ttgatatcgg cgacgtgaat
     1981 cattggggca tggccctcct tactgattcg gtgatcggct gcggcaagat ggcatcccta
     2041 cagtccatcg atgccgcctg gtgccgtagt catcctgccg ggatgacgga tgtcggagac
     2101 ggctgcctgt atcatgagcg tttggcgttg ccgggtcggg tctttatctt tggcggcggc
     2161 catgtggctc aggccctggt ccccgttctt tcgggcatcg atttttcctg tatcgtcatc
     2221 gacgaccggc cggaatttgc aaagggcgat ttatttcccc aggccgcggc ggtgcgtctg
     2281 gtcgattatc gggaactgcc ggaagacctt ggcatcacgg ccgatgatta cgtttgcgtc
     2341 atgacccgcg gccaccacgg tgattacgac gttcagcggc agatcctgcc tattcggccc
     2401 cactatctcg gcgtcatcgg cagtcgccgc aaattggcct ttgttaaagg gaaactcttg
     2461 gccgacggat tttccgaagc cgatatcgac gcctgctatg gtcccatcgg cctggctatt
     2521 tcagcggaaa cgccggctga aatcgccatt agtattgccg ctgaaatgat cgccgtccga
     2581 gccaggcggg aagggcggga aaaacaccgg cccaagtcct tatgacggcc cgtgtcgaga
     2641 cctacgtcgg ggccgggggc aagacgacga cgattttttt tcgggccttg aaggcgcggc
     2701 aattaggaaa aaaagtggct gtcgttacga cgactcatat gatgcggccg cgacagggat
     2761 tcgtccccgg tgacctgccg gcgggctgga aggagcagt
//
LOCUS       DAWDXZ010000203         2797 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7412_length_2797_cov_6.865427, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000203 DAWDXZ010000000
VERSION     DAWDXZ010000203.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7412_length_2797_cov_6.865427"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2099..2108
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          641 a          700 c          731 g          715 t
ORIGIN      
        1 agatagagtt cttggtccgt tggttacggg catctgaaaa agccactgat gactaaatca
       61 gtcgtcagtg gctttttgtc gtaaaaaatc agaaggcgaa tttcggatac ataaatcagt
      121 ctctatccgg tacgaatcga tacgccgatt tgtcagtcgc cccagtccat catatggtcg
      181 ataaaggtgt cgatccggtc gcgttggcgc tcttccggag ttttttcccg ctgggcagca
      241 tagcacggac cgtggtgatg ataggtgccg cgacggccgg cttggcggta atggggatag
      301 taaggcaggg tatcctgatg gaagaaggtt tccgtattgt acgtgtggac ggccttgttg
      361 tactggtcga cggcgtctct ccgccgggcc aggagttcgt caatctgttt atcgacgtcg
      421 cggacatagg tgtccaaatc tttccgatag gcgtccattt cggatcggct ggccatttcc
      481 gctgagaagg gttcatggct gctggaaaaa cgttctttgg catctgcgcc gatgggcagg
      541 gccattgcca gggaggctag gatggcaata gacatcattt tttttgacat agcgtgtagt
      601 ctcctttttt tacataaaat acataattat tataccttat ttttacaccg aacctcaagt
      661 tctattcgga agtcatgacc ataaggaaaa tctttccgtt tctcccaaat catctgccgg
      721 ccctgtttcg ggagagggcg ttcaaagccg ttctgactga caagagaata ggagaggatg
      781 aggaggtttc aacaggattt tcggccgaag gcatgttttt agaaaaagtt ttcaaagaag
      841 acttgaccgg tagttaatac cgaatgataa gataaatttg tacctgaggt gaaggccggg
      901 acagccccgt ttgtaagccc ttttggaagg ttcgatactt tttgttgctt ttacacagcc
      961 tcttggctgt ttatagatag atattggata ggagacgatg aaatggaaaa ctttgttttt
     1021 gaaaatagta caaaagccta ttttggaaaa ggctgcgtcg gtcaatactt gccggaactc
     1081 ttgtcccatt acgggaagac ggtcatggtg gcctatggcg gcggctccat taagcggacc
     1141 ggcatttacg atgaaatcat ggcgattttg accaaggccg aaaaggacgt cgtcgaattt
     1201 tcgggtatca tggccaatcc gacctatgcc aaggtcctcg aaggggcgaa aatcgccaaa
     1261 gaacaggacg tggacctcat cttggctgtc ggcggcggtt cggtcatgga ctgctgcaaa
     1321 gccgtgtccc tggctgccgt ctatgacggt gacgtgtgga ctgatttttg ggctaaggcc
     1381 ggcaccgtcg acgtcaaacc tattccgtta ggcattgtca tgaccgtatc cgggacgggc
     1441 agtgagatga acggcggcgc cgtcattacc aacgaagacg taaaggttaa gaccggccgc
     1501 gattacccgg ccctcaacgc tcaatttgcc ctcctcgacc cgctttatac ctacaccgtt
     1561 cctaaactgc aaatggtttc cggcgccttt gattccttta cgcacatgat ggaaacctat
     1621 ttcagcgccc ccgatatcga taatgtgtcg gacagcatta atgaagccct catgaagagc
     1681 gtcgtccgca accttcgtaa ggccattgaa aatcccgaag actacgaagc tcgcagcaac
     1741 ctggtctggc tctcgacgat ggctgaaaac cggattctca aattgggaaa gaagggcgat
     1801 ttccaaggcc atatcttaga acactccctg ggggctttta cggactgcaa ccacggctgc
     1861 ggcctggccg tcctcttgcc ggtctattac cgccacatta aagttgaagg ggcggaaaaa
     1921 ttcgcccgct ttgccagcga agtttggggc atttccccgg aagggaagac aacggccgaa
     1981 cttgctaacg acggcgtcga agccttggcc tcttttgtaa aggaaatcgg cctgccgacg
     2041 accttgcggg aactgggcat gacggacgac caaaaagatt ggctgccccg tatagcttnn
     2101 nnnnnnnncc tgctcatggc cgtcggcgcc ttggcccgtc gctatgagat cctgacgccg
     2161 gccaatcagc cgcagattac gaaattggtg gtcaacattg cttatccggc catcatctta
     2221 tcgggagtca ccggcaaggg gcccgcattg aaggggcgga actggcctat gccttcggcg
     2281 tcatcctggc catgctggtt ttgctcatgg cctgtgcctg gatcctgccg cggatccttc
     2341 gctatcccaa gaaacactac ggcatcgtca atgtcatgat catcttcacc aatatcggct
     2401 tcatgggcgt tcccatgatt gacggcattt acgggaaaga tgccctcatt tatatgaccg
     2461 tcctcttgat tccttttaat attctctttt tctcttatgt tatccagacc atcaaaggca
     2521 gttctgacaa gaaagaaccc tttcgttggc agagtttggc caataccggc atgggcgcct
     2581 gcatcctggc catcatcctt tacctgagcg gcatcgagct gccgtatata ctggcccagt
     2641 cgattaccat gctcggcagt atgacggcgc ctttggccat gatgctcctc ggttctttcc
     2701 tgaccgatac ggcgtggaag ggcatgataa acggccgcat catcgccttt acgatcatca
     2761 agatggtcgt cattcccatc gtcctgacct taattct
//
LOCUS       DAWDXZ010000204         2795 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7418_length_2795_cov_6.463504, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000204 DAWDXZ010000000
VERSION     DAWDXZ010000204.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7418_length_2795_cov_6.463504"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          588 a          738 c          735 g          734 t
ORIGIN      
        1 ctgcggcgct tgccttcaaa taagaacggt ttggtaacga cagcgaccgt gagggcgccc
       61 atttccttag cacattcagc tacgataggg gctgcaccgg taccggtacc gccgcccatg
      121 ccggccgtaa cgaataccat atcggcacct tggagagcct tgatgatttc ttccttgctt
      181 tcaagggcag cctgttcacc aacctgagga ttggcaccgg cgccgaggcc gcgtgtcaat
      241 ttttcaccga tctgaatttt gacatcggct tgggatactt ccaacacctg gtcttccgtg
      301 ttgaccgaaa tgaactggac cccctgcaaa ccgctttcta tcatacggtt gacagcgttg
      361 ttaccgccgc cgccgacacc aattactttt atattcgcaa actctgccat atcgcatttt
      421 cctccttcgg actctatacg cacaccttgt gcagtaggat tctttattat tttacactat
      481 cttcgaaaat ttatctagct gtttttaaaa atcaaggcct ccagcctgtt tattttgcct
      541 taatagccgg tgctctgagg tcggtatcga tatactgtac attgatcccg ttgctttgag
      601 cttcggtaag taaggtttcg gtcatcttgg cccgttcagc cgggtcatct cccttaccga
      661 ggcggatagg aacggaatca gtcgtatagg cgacgatact ttcgggatta ccgacgttta
      721 cttcggcaat cgttttaagc aggtccggcg gcagactctg aaggtatatc atagccgctt
      781 gaagcgtcgg atcgtcaatc gtatcgccaa ggagcaggtt gtccactttt ttcccggtca
      841 tgatgggcag ggaaacgcct ttgatctgcg gttccaaatc gatgacccgt cccgtcttat
      901 cgacgtaagc gaaaccatag agcgtcgtaa tgatagctgc cggcttgcgt tcggtcatct
      961 cgatgtgaat ggtcgccgga aattcccggg ttacggtggc ctcggcaatg cgcaggtctt
     1021 ttttcagccg ctcttcgatg ccggctgtcg acagttggac gacgttgacg taggaataag
     1081 cgccggcggc gcggtaaaca tcgtcaaagg acatgttttc attgccgtcg acgatgattg
     1141 tgccaaaggg aaccggtgcc agccgcagga acagccacaa ggcaaagacg ccgaggacgc
     1201 ctaagatgat cgtgcatcgg cgccggcggg cacgcgcttt tttctgctgc tttctttttt
     1261 tcttcagtcg ggcggcctgc cgtatctgct gacggcggcg ttcctcttct tcagccagtc
     1321 tcatatcctc atcgctgcgc aagggatccc tgtccagatg aggaggcaca aagacatcat
     1381 gaggatcatt cggcactacc gttcgcggcc gccttcccgg atatggatgc tgtttcatca
     1441 gaatttacct accgatgctt cgagcaaaat cttttcgcac aaatcttcaa aagaaagtcc
     1501 catggcccgt gctgcgtcgg ggaccaggct ggtcgccgtc attcccggta cggtattgat
     1561 ttcaaggaca aagggatggc cttctttatc ggtcatcata tcgacacggg acacaccgct
     1621 gcagtggcag aggcggaaga cttttaaagc caggtcctgc atctcttttg tgagagccgg
     1681 tgaaatggga gccgggcaga tgtgcttggc aatgcccggt tcatattttg aatagaaatc
     1741 gtatttcccc gtactggata cgatctgaat gaccggaaag gccaggtcgt ccatgacggc
     1801 aacggtaaat tcatcgccgt cgatgaaggc ttcggctacg atggatgtgc tgtacttaaa
     1861 ggcttcttta agggccgatt ccagttcttc ccgggcggtg acgatgaccg ttccgatgct
     1921 cgatccctgg ccggacgact tgatgacgac gggcagagaa aactgctttt caatatcggc
     1981 gatgatctcc ttgcggtcct gattggcaaa gtagtaggca aaaggagccg tggcaatgcc
     2041 ggcgccttta aaggcagcct tgctcataac cttattcatg ccgacggcac tggccatgac
     2101 gcccggaccg gtataaggga tgcccatcat ttcgcacagg ccttggagaa caccgtcttc
     2161 gccgtagcgg ccgtgaaggg cgttgaatac gacgtcgatt ttcttttctt tcaaggttgc
     2221 cgccaaggta tgcggaacga gttcaatcgt ttcgatggca tagccttttt tcttcagtgc
     2281 ttcggcaatg gcagctcccg tgcggcgcga tacttccgct tctgtcgaag ggccgccgac
     2341 gacgacggca atctttttac ctttcatgtc ttccatgcgt tatttctcca tttctttcgc
     2401 ttcggcaata tattgttcgc cgactttata aatatctccg gcgcccatgg tgatgactaa
     2461 atcgagaggc ttggagtttt ctttcaaata ggcaatcaaa tcctctttat gaggaatgta
     2521 ggtgacgtcc tgtcccgtcg tctccttgac ttttttggca atgagttcgc ccgatacgcc
     2581 gggaatggga tcttcaccgg cactgtatac atcggttacg atcagtttat cggcctttgc
     2641 aaaggcactg ccgtattgtt cgaggaggag cttcgtccgc gtatagcggt gcggctggaa
     2701 gagacagacg acgcggtggg tccccatgtc gcgagccgcc gtcaaggtcg cattgatttc
     2761 cgtcggatga tgagcgtagt catcgacgac ccaaa
//
LOCUS       DAWDXZ010000205         2784 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7436_length_2784_cov_8.440088, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000205 DAWDXZ010000000
VERSION     DAWDXZ010000205.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7436_length_2784_cov_8.440088"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          653 a          691 c          725 g          715 t
ORIGIN      
        1 gtatcagtat gctcacatgc tggttctgaa caatgccgaa tgggtcgatg aggcggcttt
       61 tccgcttctg cggcgtcgca tgtattcgac gctgtacaac cccaacttgc tgtcggcctt
      121 cctgttgatc gtcatgagcg cttcagcatc gatgatgatc tgcacccgcc attactggca
      181 ccgggtcatg tacttggtgc tcctggccat cctgaccctt tgcctggttt tgacctactc
      241 ccgcggggcc tggctcagtg tcggcgcctt ggtctttttc tttggccttt tttgggacaa
      301 gagggtatgg ctcctctttt tggcagcgcc tttgatcgga gccttttatc atggcggtgt
      361 cgccgaccgg ctcttatcga tttttagcca cagtgaagcc gatacgtctg ttgccatgcg
      421 catggatatg tgggaagcgg ccattgctat gtttctcgac catcccttgt tcggcatcgg
      481 ttggggggct tttaaacacg tttatcccgt gtacaatgaa ctgatacagg aagcgggcat
      541 cgtcatcttc cacgcccaca atatgtatct caatttcttg gcggaaacgg ggctggtcgg
      601 tacctttttc ggcctgtggt ttttcttcgg aaatgcttgg tacgctattc gttacgtaaa
      661 agatcatcat gttcggactt ttgaccggtc cctttccatg accttggcgg cgtccgtcct
      721 gtcgctttcc atcagtggaa tcagtgatta tgatttgttc agcacgcaaa tatcgctcac
      781 tttttggttg atgagtgccc tcttcgccaa tatgtttggg gaactatgtg aaaaaagtaa
      841 taaaaacagt ttgcgaaata attcacaatg atatataata ggcatgtgac tattatcact
      901 agacaagatt gcttatagct atagggaata gctggggaca tagttggagg gttactatcc
      961 atgagacaga aaaaggcaat atcggatgct gtcatcgaac ggttgccgct gtactacagg
     1021 gacttgctat tactgcaaga tgcgggaatc gatatcatct catccgaaaa attaggggaa
     1081 cgcttaggca tcacaccgga acagattcgc aaggacctta cctgctttgg tgcctttgga
     1141 aaaaaaggcg tcggttatta tgtcagtgaa ttgtgtcaac agatcgtgga aatcatcggt
     1201 ttgcagcagc attggaaagt cggcatcgcc ggcatcggcc atctgggctg ggccttggcg
     1261 aactataaga ccttcggtcg tttgggtttc cggacgacag gactcttcga tgtggaccat
     1321 cgcattatcg gccaaaacgt cggcggtgtc gaagtcacgc cgatttctga aatgggggac
     1381 gtcatccgtc gcgaaggcat ccagatcggc atcattacga cgccggcagc cgttgcccag
     1441 caggtggcag atcaaatggt cgatgccggt atccgcggta ttttgtcttt cgcaccgctc
     1501 agattgaccg taccggacgg gattttcgtc tttcgagaag atttgtccat cggcctgagc
     1561 cgtatttcat actatctgac caataagaca gaatgaagat cgttgcaaaa cgattgtata
     1621 aactttcata aaagaattta aacagtatct aaaaaggatg gtgcccgtgt gcggcaccgt
     1681 ccttttttat ggtataatac tcgtactagt tagtgaatga ggtgatttta tggacagcac
     1741 atccatttat ggtgcatatg aaccggctct cgagtccgtg atgcaggacc ttatcggccg
     1801 tatccgggcc tatagcagcg ataccaagaa aaagaccggt gaagccgctt acgagcattt
     1861 gatctaccgc ctgaagggcg aggccagcat gcgggaaaag tgcgaccgca aagggctgcc
     1921 ccagacagca cagtcggcgc tccaccatat atacgatgcc atcggcatcc gcattgtctg
     1981 ttgttttctc gacgatattt atcgagccat tgattttttg aagagtcagc ccgacattcg
     2041 cgtcgtcgaa gaaaaagatt atatccgcaa cgtcaagccc aacggttatc gcagttatca
     2101 tctgatttta gaactgcagg tgccctatga agatgtaacc ggcgctaatc cgggccgctt
     2161 ttatgccgaa gtccagctta ggaccattgc catggattcc tgggccagcc tggaacacca
     2221 gatgaagtac aagcaagata tcaaaaatcc ccagctcatc atccgggaat taaagcgctg
     2281 tgccgatgag ctggcgtcgt gcgacgtgtc catgcagacc atccgcaatt tgatccatgc
     2341 acaagaataa ggaggaatga agatgcgcct tctcttagca gaagatgaaa aagaaatgtc
     2401 cgcggccctg gtcgcgattt tgactcattc gggctatgag gtcgatgccg tctatgacgg
     2461 ccaggccgcc gtcgataagg ccctggtcca gtcctatgac tgtatgatcc tcgacatcat
     2521 gatgccgaaa aaagacggcg ttcaggccct gcaggaaatc cgggaaaaag gggatatgac
     2581 gccggtcctc atgctgacgg ctaaggccga agtcgatgac cgcatcaccg gtctcgacgc
     2641 cggcgccgac gactacctga ccaaaccttt tgccatggga gaactcctgg cccgcctgcg
     2701 ctcgttgacg cggcggtccc agtcctttac gccgtcgaag ctccacgtcg gagccgtcaa
     2761 actcgacgtg gatgaacagg aaat
//
LOCUS       DAWDXZ010000206         2781 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7442_length_2781_cov_8.060161, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000206 DAWDXZ010000000
VERSION     DAWDXZ010000206.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7442_length_2781_cov_8.060161"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1591..1647
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          744 a          631 c          783 g          566 t
ORIGIN      
        1 tgttcttcca tctctatcac ctctatatcg tatggctgtt tacgccggcc tggtttacga
       61 cagcagcatg gcctaaggct gtattgtcta agggagaggt ctgaacccct tcaaaggggc
      121 tcccggcggt gatggcgtaa ttgtaaccct gttcgccgtg ttcgccaata tcgagaccgg
      181 caatttcttc ttctttatcg gcccggacgg tcatgaacag gctgatgact ttaaagatta
      241 tgaatgttcc gacgatagcc agcgcatagg cgacgaccgt agaaatgagc tgagcggcga
      301 ggagatggct gctgccgtag aagaggccgt cggcaccggc agggttgacg tctgtcgtcg
      361 cccagagccc cgttgcgatg gagccccaag taccgccgat gccgtgaatc ccgaaggcat
      421 cgagggaatc gtcgtagccg agtttggtct tgacgacggc gaccgcgacg tagcagataa
      481 cgccgccgac agccccgata atgagggccg gaaggggagc gacgaagcct gctgccgggg
      541 tgatggcgac gaggcctgcg atacatccgg aagcggcacc gaggatggtc ggtttccctt
      601 ggacacgcca ttccacagcg acccaggcga cggtggcagc tgctgcggcg gcctgagagg
      661 tgataaaggc gttggcggcc aactcgttgg ctccgagggc actgccggca ttgaagccga
      721 accagccgaa ccagagaagg gcggcgccaa ggacggtcat cggaagatta tgaggaacca
      781 taggactctt cccgtatccg tagcgacggc ctaagaggac gcagagcgta aggccggaaa
      841 caccggagag aatgtggatg accagaccgc cggcgaagtc gagggcgccg agtttagcga
      901 ggaagccgcc gccccagacc caatgagcca tgggattata aatgaagagg gcccagagca
      961 ggatgaagac gacgaaggcg tggaacttca tgcgttcggc aaaagcaccg gtaatgaggg
     1021 ccggcgtgat gacggcaaac atgcactgga aggcgacgaa ggccagttcc ggaatcgttc
     1081 cgttgtcgag aacagtcatg ccgactccgg tgaggccgat tttatcaaga gatccgatga
     1141 agccgtgaat gtcggtaccg aaggtcatgg catagccgat gagaatccat tcgacagaaa
     1201 tcatggcgac gatgaagaag ctctgcataa tcgttgccaa tacgttttta ttgcggacca
     1261 tgccgccgta gaagaagccc aggcccggcg tcatgagaaa gacgagggcg gcactgatga
     1321 gtacccaggc cgtgtcgccc gggtctgtcg ttcccggtgc cgatgcaaaa gccgttggcg
     1381 ctgctgcgaa aagacaaggc agcaggaccg ataaacggga ccataattgt ttcatattct
     1441 tagcctcctg acccataaaa aaaagatgcc tctcccgatg gttccatcga gaaaaagaca
     1501 tcgttgtcta ctgttctatt gggattatct tcagaatatg aaaagcccaa gggataaaaa
     1561 atcatcacct tgggcttcat tgccggcttc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnntga acttattata aaagcaaaac gttaagctgt
     1681 caataaaaat gctgaaattt attttacaaa acttatattt tatctaaaaa gataatatca
     1741 taatgggaat catattaaac aaataaaata tgccctaaga aaatataaaa ggtcagcgat
     1801 atggcgctga gggccgttgt gttcatgaca atctgcgtcg catagtccgg attgttatcg
     1861 aattcgatgg caaataaggc cgtattgacg gctgtaggga tagcgcaata gataaggaat
     1921 accgtcgcac tgacagccgt aaaggtgccg gggcgaaagg cgttggcacc ataaatcatg
     1981 gccagcccca gtaaggggag ggcggtcatc tttaaaatgc ttacgatcca gacatccttg
     2041 tcgagccaat gaatcttggt ctgtgacaac tgagcgccga tactgaccat ggctaagggg
     2101 agcagagcat ggctggccat gtccatgatg ggccataaga agaagttttc ggcatcccag
     2161 ccgacgttgc gggctaacaa ggatgcggcc aatacgtaca gcaagggcat gcgaaacatg
     2221 acggccaaag tatccctggc agccaattta ccgcggccgg cctgataaag accgagggta
     2281 ttgagcgaga cactttgaat catgaatatg atgatctgca cgaccatggc ttcggcaagg
     2341 tatggcgtct gtccgtttac gacgaagggg tcgtgagaaa agacgaggat gataagcgcg
     2401 acgccgaggt tgcctgtatt attgaacata aaggcgttgc ggcacgattc cagcatggct
     2461 tcatcatagt ggcggatacg gccgacgata ttggctatga cgaaacagag gaccatgaag
     2521 atggcgatgg cagcgatgat gttgacgctc gtttcgggga actgagtcgt atagatgttg
     2581 gtaaaaagga aactgggaat gactaagtag aacatcaatt tcgataaggt ttttacgtcg
     2641 agatggaatt tcttatcgag aaaatatcct aagagaacga gaaagaaaat cggaagcatc
     2701 gtatgttcaa tgatgaacca gaagaccatg agtcatatca ctccttatta tatggatact
     2761 ccctcattat actatggata t
//
LOCUS       DAWDXZ010000207         2775 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7455_length_2775_cov_10.277206, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000207 DAWDXZ010000000
VERSION     DAWDXZ010000207.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7455_length_2775_cov_10.277206"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    577..586
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1931..1940
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          494 a          767 c          786 g          708 t
ORIGIN      
        1 gcggacttat ggaaaaagtc ggcgttgata tcgtcttatc gacgcggctc ttgtcggccg
       61 gtgaagtcct ggcctttgcc cggggcggcg tcgtatcagt ctctctcctc gaaggagcca
      121 aggctgaagc tgtcgaagtc atcgtccagg aaggggcacc ggtcatcggc aaacgactga
      181 tggatctcag cctgcccaag gaatgcctga tctgcgccta tgtccgcgac ggggaagcgt
      241 acattccgaa cggccagtcg gtcctcttgg aaggcgaccg catcatcctc ttcagtcaga
      301 cagaacattc gcaaaaggtc atgcagtact ttaaaggcag ggagtagcat ggacagacat
      361 atcttgttct atctcgtcgg caggatcatc ctggtcaacg gactgagcct gatcattcct
      421 ttgacagccg gccttctttg gggcgaaagg ggactcttat acttcgggcc ttccctcatc
      481 gccgccgtgg ccttagcgtt tttttgcctc tatcgcggcc gccatcataa gcgccaggtc
      541 ggggccggcg agggtgcctg gtatatggtc ctcgtcnnnn nnnnnnctcg tctggctggc
      601 attgagcctg ttggggatgg tgccgtacct tattgccggg gtctttacct cgccggccga
      661 tgctttcttt gaaagtgcag cagcctttac gacgacgggg acgtccgttc ttgccgacgg
      721 cggtgccgcc cttccgtcgt cgctcctctt atggcacagt ctcatgggtt ggctgggggg
      781 cttaaacttt atcctcatgc tggtttcggt catgcccttg gtgagcggct gtttcggcct
      841 gaccttatcg gtccatcaaa gcgtgtcttt cagtcctatg gtggccagta tgcaggacgc
      901 agcccgaaag gtaggatgca tctatttggc cattacgatg ttttctatct ttctctattg
      961 gctggccggc ctttcgccga cggcagccgt caatcaagcc cttctgacct tgtcgacgag
     1021 cggcggcgat tcgctcttcg attttttccg ctatgacagt cgggccctcg aaggggcggc
     1081 ggccctttcc atgctcctgg ccagcggcaa cttcctcttg tactggaagg gctgggaacg
     1141 gcgcagtctg aaaatctttc tgcacgacgc cgaactgcgg tattttttga tgatgttcct
     1201 cggcgtcagt gccgtcgtat ccttccacct ctggcactac ggtgtctatg atggctggga
     1261 taatctgcgc tacggctttt ttcaggtctt atcctttatc agtaccagtg gttttgcatc
     1321 gacgtcgatg gagtcctggc cggaatttga taagtatgtc ctgtttttac tggcctttat
     1381 cggcggctgt atcggatcgt ccacaggggg gctcaaggtc atgcgcttta tggtgctgtt
     1441 taaaatgacc tcccgggaat tgcagcggac cctccacccg catatggtcg tttctttgaa
     1501 aatagacggc gttcccgtcg atatgaaaat tgtcagccgc gtcctcagtt actttttttt
     1561 agtcatcatg accttttttg catcgatgct gatcatttcc ttagccggcg tcaccccgat
     1621 tcaggccatg gccatggccg tcggctgtct ctccagtgtc ggttcgacga tggccctctt
     1681 tggtgtcacc gatgtggcat cgctaccggc gtggacgaag gtatactgcg ggtttttaat
     1741 gattttaggc cgcattgaaa tatttcccgc cctgctggtc attcagcaca gtgtcagtta
     1801 tgtctggcgc cgctggtaag ccttggtaag aatgtgctgt ggacggaaac ctttccgtta
     1861 aaaaacaatt aaatcacctt agaccatctc cttttcatgc tatgctggca gggaaaggag
     1921 atttttttat nnnnnnnnnn tgaacgcctt atttcaacaa gccgtcgcgg ccggtgccag
     1981 tgacattcat atcgaaccct tggatgaatt cgtccgcatc cgctggcgaa aagacggggt
     2041 cctccatgcg gcagggcaat tgcctcgcca gcgccttgac agcctcgtca gccgcatcaa
     2101 ggtcttagcc ggtctcgaca ttgccaatcg gcgcctgcct caggacgggc gcatcgtttg
     2161 gcatgaggga agtcggcgtc tcgaccttcg catcagcacc ttgccgacgg ttcgcgggga
     2221 gaaaatcgtc gtccgcatcc tcgacggcaa gcggatgccc ttggagttgt cagctcttgg
     2281 catggacggc gaagcccggg cctgtttaga gaaattggtc tgccgccggc aggggctgat
     2341 cctgatttgc ggtccgacgg ggagcggcaa gacgacgacc ctctacgccg cccttcgcca
     2401 tattcacgat gagcatctca gcattgctac cttagaagac ccggtagaaa tattgatcga
     2461 cggcctgtct cagagtcagg tgcagccaaa aggcggtatg gtctttcaaa acgggctgcg
     2521 ggccctcttg cgtcaggacc cggacgtcct cgtcgtcggc gagatccgcg acggggaaac
     2581 ggcgcagatt gccgtccggg cggcccttac gggacatgtc gtgttcagta cccttcatgc
     2641 gccgagtgcc gtcgaagcgg ccgtccgctt gagcgatatg ggcgttccgg cctacttagc
     2701 tgccgatgcc ttgacgggca tcgtatctca gcgcctcgtt cgccgtaaga cagcttccgg
     2761 ggattatgaa gggcg
//
LOCUS       DAWDXZ010000208         2769 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7470_length_2769_cov_7.146279, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000208 DAWDXZ010000000
VERSION     DAWDXZ010000208.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7470_length_2769_cov_7.146279"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    502..511
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2243..2252
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          624 a          736 c          760 g          629 t
ORIGIN      
        1 gatcttcatc gagttctttc atgtaatgga agttgatcca gttcaatttt tcaatatcga
       61 agacagccgg attcttagcc acgcggctca gggagaactg ttcgttcaat tcgtcgcggc
      121 tgaagatttc ctggtcgctg tccggcgtcc aaccgaggag agccaggaag tttaccaggg
      181 cgtcaggcag atagcccata tcgcggtact gctgaacaga agtggcgccg tggcgcttac
      241 tcatcttctt cttgtcggcg ccgaggatca gcgatacgtg ggcaaactgc ggggcttcaa
      301 agccaagcgc tttatagatg accagctgac gcggcgtatt cgacaagtgt tcttcagcac
      361 ggatgacatg ggtaatgtgc atcagggcat cgtcgaggac gacacagtag ttgtagaccg
      421 gaatgccgtc ggatttgacg atgacgaagt cgccgacgtt gttggagtcg aaggacacgt
      481 ggcctcggac caggtcgtcg annnnnnnnn naggtaatgc ccagccattt caaagcttct
      541 ttaatatttt cttccgattc ggacgtcgaa cgtttgcggt ccgtatcttc gatgcggacg
      601 acgaacttgc cgttcgtgtg tttagcgaca aggtagttga acagtgccga acgggcgccg
      661 ccgatatgga acggtcccgt cggactcgga gcaaaacgaa cgcgtacttc ctgtgacata
      721 aaaaattcct cctaaataca tacttatagg tacgattgat agcttaggat catggccgcc
      781 gcttgagctg ccatgccttc ttcccggccg acgaatccta atttttcggt cgtcgtcgct
      841 ttgacggaaa cggccgactt agaaatccgc agggcctcgg caatattttc aatcatctgc
      901 ggcacaaagg gtttgatttt aggctgctgt gccaacagcg ttacgtcaag attgacgatg
      961 cggcagcact tttcctgcaa gagatggcgg accttagaga gcagcaccag gctggaaatg
     1021 tctttaaagg tctcatctgt atcgggaaaa taggtcccga tatcacgaag ccctgccgcg
     1081 cctaataagg catccatgac ggcgtgaata aggacgtcgg catcggagtg gccttcaagg
     1141 ccccgatcaa agggaatctc gacgccgccg agaatgagcg gccgcccttt gatcaggcgg
     1201 tggacatcgt aaccggtgcc gatgcgctgg acctccctcc ctttcaaata cagggacagg
     1261 atgggaatgt cgtcgcgggt cgtcacctta cggttcttat cgctgccttc aaggatgacg
     1321 acgggtacgc ccatggcctc gacgagggac gagtcgtccg tcgccttgag tcctttcagc
     1381 cgcgcctgtt tatgggcttc catcaataag tcccgatgga agatctgcgg cgtctgggcc
     1441 gccactaagc ggctgcggtt cagcgtatgg agccggccgt cgtcaccgcg aactttaatg
     1501 gtatccttta agggaatccc gtagatggcg gcccgagccg tttccatcat gaccgctccc
     1561 tgatcatacc aatcaggcgg cgcaaaggga cgggctccgt cgtggaccat gacgacatcc
     1621 atatcggagc cgacggccaa gagcccgtta tacaccgaat cctctcgttc cgccccgcct
     1681 aaggtatagc gcacaggctg aatgggatgg agatcatcga cgatacggcg gatctccttg
     1741 gtatcgatgc gggaatggac gatgaccact tcatggaccg agcgggctgc gaagatgttc
     1801 cgcagggtat gggtcaggac aggttcgccg cacagcggat aaaagagctt attccgctcg
     1861 taaccgaagc ggctccccgt gcctgcggct accacgatga cagatacttt cacacatttc
     1921 acctactcat ttgcgatttt tacaaatatc atgcggccgg cagacgtctg caggacagac
     1981 gtaaccgtaa ccggcatagt cgagccgata tatttctgac cgtcttcgac gacgatcatc
     2041 gtaccgtcgt cgaggtaagc aacaccttgg ttttcttcct tgccggcctt gacgatgaga
     2101 acattcatct gctcaccggg aacgcgggcc ggtttcaacg cgttggctaa atcgttgatg
     2161 ttgaggacgg cgacgccttg aaggccggca accttattca ggttgtagtc gttggtgacg
     2221 accttggcat tgattttacg gcnnnnnnnn nncccaggcg gacgagcttg gagtcgacgc
     2281 cctggacgtc gtcaaaatcg tcattgatga tgcgtacttc gacgtagttg tcttcctgca
     2341 tctgctttaa gatatcgagg ccgcggcgtc ccttattgcg cttcaataag tccgacgaat
     2401 cggcaatgag ctggagctct tccaaaacga ataccggcac caagagcggt ccttcgagaa
     2461 aaccggtgct gcagatctcg gcgatgcggc cgtcgatgat ggcactcgta tcgaggagct
     2521 tcccggaaaa ttccagttcc ctgcgcttgt gctccttgtt cggacgaccc agacgcaggt
     2581 tgccgacgag ggcaatgatt tcactcttct tgcggacggc aatgctcatg ccggcatacc
     2641 caaagacgat attgaataca atcggtccgt aagcgccaat aatcggcagt cgagcgaagg
     2701 ccaatccaat taaatttgca atgataagtc caaaaagcaa acctaacgtc ccgacgatga
     2761 tatcctggc
//
LOCUS       DAWDXZ010000209         2767 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7479_length_2767_cov_6.667773, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000209 DAWDXZ010000000
VERSION     DAWDXZ010000209.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7479_length_2767_cov_6.667773"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1295..1304
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2016..2025
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          788 a          556 c          686 g          717 t
ORIGIN      
        1 ttttgccggc ccatacagtc tgcttgtttt tggaaaatca attttctcca caggacttgt
       61 tctctacgca ggaagatgaa attctttaca atacgctaaa agttttaggc aagtataaga
      121 tggttaaatc ggttggatat ggtaaagaat tagccttgga tgaagctaaa tgtttgaaca
      181 tattaaatgg tcatcctgag gcggctgatt tgaaaaatat tattatgcgc tatggggagc
      241 aggagcgcat caaagttgca acaatcgctg caggggagtt aaaaaatcaa ttgcttcagt
      301 gtgactacca gagggccatg ctcgataaat acgatgaaaa attgttgacc gatcctaagc
      361 gcgggcattg ggatttgtgg gattgggatg attttcataa agacggcgtc aaggtgacgc
      421 tgccggagcg ctgggttgcc cgtaatccgg aagaagatat caaccagggc attgtagcca
      481 ttgattttgg aactaagagc accgtcgtcg tctatgaaaa tgaacagtta cagcgattgc
      541 ccttacaagt gggcaacggc agttatcgtg ggggcgtcga cttgaataac tatgaaaatc
      601 cgacggttat tcagtttata gatctgcagt cctttataaa ggcttataat gctagagatg
      661 gccggccgta tacgagctgg aatgatatga ccgtgtccca tactgctttt caaaatatga
      721 ataataattc tgaaggactc tattattctt tcttgactaa cttaaaacag tggtgcggca
      781 acggaaagcc cttggttttg cgggacaaaa aaagattcga caaggattta cctccctatc
      841 aagatttgga tggagacgaa gaggccgatc cattggctta ttatgcctac tatttaggcc
      901 tatatatcaa caacatgatt tctgaaaagc atctgtttat gcactatatc atgtccttcc
      961 cggtcacgta tgaaaaagag attcgtgaaa aaatgctgca cagttttgaa gtggggttga
     1021 agaagtcttt gccgacggct attttgtcga attctgaagc catgaagcgc ttctccgtag
     1081 aagtcggtgc ttctgaaccg gcggcctatg ccattacggt gcttcaggaa tacggttttg
     1141 atcccagcgg tgatgaaaag atttactatg ccgtctttga ctttggcggc gggacgacgg
     1201 actttgatta cggcttttat caagaagccg acagcgagcg ctatgactac aaattagttc
     1261 attttggaga aaatggcgat cggacgctag gcggnnnnnn nnnncttaag cctcttggcc
     1321 tttgaagtct tcgttcataa taagaaaaaa ctgctcgaat cgcgcattcc ctttacttgg
     1381 tctgccgata aatcagactt ccaagggagc gaggccttta tcaaagattc gcaggaagct
     1441 catgctaata tgcataattt gatggaagct ctgcgcccct tatgggaaaa tccccgcggt
     1501 gaaaaagctc aaaagatgca ggaagagggc actgtttcct tccatgcctt tagtgaagat
     1561 ggacagatga atgccgattt tattctgcgc cttgatggtc aagatgggca gcctttagat
     1621 ttagaacaaa tcattcagga ccgtatcttg aaaggcatca aaaatttctt catggctatg
     1681 aaggaatcct tcgaacagta tagcggtgaa aacggtatcg agtccttgga tgaggttccg
     1741 gccattgcca tcttcttagg cggcaattct tcaaaatcgg aacgagttaa gcggttgttc
     1801 ggtcaggtac tggggctgga aaggaaagcg attcctgagg atgaggtcgc tcccgacgag
     1861 acttcccctg aaatatctga tgaaatgctt tgggacatga ttaagaaaat ggatgaagat
     1921 gttcttgagc aagaaattga ggatatattt gaggaaataa tgggggcgga attcgttgaa
     1981 aaaagtcctg acaattttcg gaagcagctt tccgtnnnnn nnnnntttcc gttcagttac
     2041 agaagatgac ttctaatagc ctgcttacta aagaggtcaa agaaaacccc gaaagactga
     2101 tagaaatttt aatatatatc atcccttggg aaacccctga gacgctgaca gcctatctta
     2161 aagagagaat gattgattat tattttaagg cacaagaacg gagtgctgcg cagaaaaaag
     2221 caaagagagc ctcgtcaatg agcgcgtcag agacctctct tgtcatgcag tggctgggcg
     2281 ccgataaata cgggaagatt ccggaattta cgatttaccc ggccctaggc acggaggaag
     2341 cggtggaacg acaagaagct atctggaaag aacatccgga attaaaaccg gaagaactgg
     2401 tacgtccgac gggcaagacc ggtgtcgctt acggcctgtt atcttgccgg aacagcggga
     2461 ccgttcaagt cgttaccctc ttgcaggatg aagaacgtac gcccttccaa ttctatgtgg
     2521 gccgaaataa gaagcggcgc ttccatccga tcatcaatcg aaatacgcca ttcaatacgt
     2581 ggcattattt cattgatgcc agcaatgact ttgacttgtt atacacgaaa atgccggagg
     2641 ccatgggcgg caacgtttcg gcgacgattg ccaagcagat ccacgtcatt ttggaacatc
     2701 ccgacaagga agccaatgtc tatatcgggc cggtgaatgc caataccgtg gcctacgtcg
     2761 ttgccaa
//
LOCUS       DAWDXZ010000210         2759 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7498_length_2759_cov_7.504068, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000210 DAWDXZ010000000
VERSION     DAWDXZ010000210.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7498_length_2759_cov_7.504068"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    842..851
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          746 a          619 c          712 g          672 t
ORIGIN      
        1 cggaatactg gtttgacgag gacagcaaga tacagcactg ggataatatc ctccatggag
       61 tgtttcaatg acaaaggata gaagcatatt cataaaaaac atctattata tgttgtccta
      121 cgccttcacc atactaaaac aatctaccta cgagactatc gagaaagagc cttttgataa
      181 tatccataac ctctttgcgg cgattctggc taaaggaatc ggacggcagc tcaagcaagg
      241 cctttatcga gagtataccc atcgcgtgga aaatcggcac gtcgttcgtg ggaaaattaa
      301 tatgcccggg acgatgagaa accgcttgca gcaccgcctg gttgttacct gtgactatgg
      361 agaactttcc gaggacaatc ggctcaatca gattctaaaa tcgacggtca tcctgctgtt
      421 gaaacagaag gatgtacagg acaagtacaa ggcagaactc aagaaggaaa tgctgttttt
      481 ttcgaatgta gccgagattg atctaaaaca ggtccgcttt tcggatattc gctttcagcg
      541 caacaaccgc acatatcaga tgctgcttgc catttcccaa cttctgacag aaggttcact
      601 tctgacaaca gaaccgggcg agtaccgtct gatgggattt ttggatgggc agcggatgag
      661 ccggttgtat gaaaaattca ttctcgaata ttatgcacag gaatttggac agcggataaa
      721 agggttctcg gcccgggcgt cgcagatacc ttggcagctc gacgacggct ttgatgcccg
      781 gcttccgatc atgcagacag atattacgtt gacgtacgga ggtcagaccc tcattatcga
      841 tnnnnnnnnn ncggatatgg cagtcacgtt ttgatgtaga gacccttcat tctgggaatc
      901 tgtaccagat ttttacgtat gtgaagaaca aagacgcagc agttgccgga acgggacatc
      961 aagtttccgg aatgctgctt tatgcaaaga ccgaggaaga tattgtgccg gacaatgtct
     1021 atcatatgag tgggaatcag atttctgtaa aaacactgga cttgaatcag gaatttccga
     1081 agattgcaga acagctggac cgtattgtaa gcgaccattt cgggatatgg taaattcctt
     1141 tgcaatttat gggactggat cagtccgaaa gcaggaaaat atatttcttt gtcgaaatat
     1201 ataagcatat gtcattatct atcgcttatt tttagatggg agggggaatc ccttatgaaa
     1261 aagtgtatga tggcggccct gtttatggcg gccttggcag tgagtccggc ttttgctttt
     1321 aatgaaggtc cggtcatggg cccttatgta tcggcaccgg gattgacggc aatccaacag
     1381 tgtaagctta ccgttgccgg aaaggtcatg gatcccgccg acggagccgt cctgcaaaaa
     1441 ggcgatgtgg tcatgattcc gctgcgtaag gttgccgaac agttaggtta tacggtaaat
     1501 tgggatccga ctgaccagag tgtccgcgtc gaaatgaaca ttgcctatat catcatcaag
     1561 cccggagaag actggtacga acgcatcggg acgattaaga agattaattt gaaccgcatc
     1621 ttccagtatg gcacgggacc ggtcaatgtc aacggcacga tgtacgtacc ggcacagctt
     1681 tttgaagccc tttataataa tgtgtctatt tctccgcagg acgtaacgat tacgccggcc
     1741 gaccaggttt aaaaaacatt cggttccacg gaagaccctg aaaaaattct tgactttata
     1801 ggccatggcc gataaaatag atagagaata tacgacagtc ctgtgaggct gtgtgattct
     1861 tatacttcta gcttgtacct ttaatatagt ccggagaggc tacaaaggga catattttgc
     1921 catgcttaga atatgacctt tgccttgcgg cagaggtctt ttttttcaag gaggattcga
     1981 tggttaatat acagggcaag agcctgttgg gactccaggg agtgccgagg gaagaaattg
     2041 aattgatctt gcgggttgca aagaaaatga aggccgtcgt caagagtgac aataagaaat
     2101 tgtccttgct caaaggcaag tcggtggtca acatgttttg ggaaccgagt acccgtaccc
     2161 gcggttcttt tgaaatggct gccaagtatc tcggagccga cgtcatcaat tttacgcccc
     2221 ggggaagttc cattcaaaaa ggggaaagct tccgcgacac cctcttaacg gtgacagcca
     2281 tgggggtcga tgccatcgtc atgcgtcaga agcaggaagg ctcgccgagc tttgctcagc
     2341 gctgcgtcga tccgatcatc atcaacgccg gtgacggtgc tcacgaacat ccgacgcagg
     2401 gactcctcga catgttcacc attcaggaag taaaaggccg catcgaaggg ttgaaggtcg
     2461 tcatcgtcgg cgacatcgac cacggccgtg tagcacggtc caacatttac ggcctcaaga
     2521 cgatgggggc agacgtacac ctcgtcggtc cgcggacgct gctccagccg gaattggaag
     2581 ccatgggcgt caccgttcat tacgacttga aggaagccct caaggatgcg gacgtcatca
     2641 acgtcctgcg tatccagaag gaacgccagg gtatcggatt cttccccagc gccggtgaat
     2701 ataactcgct gtttggtatc aagaaagacc gcctcaaatg ggcgaaagac gacgttctc
//
LOCUS       DAWDXZ010000211         2758 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7501_length_2758_cov_7.286718, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000211 DAWDXZ010000000
VERSION     DAWDXZ010000211.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7501_length_2758_cov_7.286718"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1229..1238
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1620..1740
                     /estimated_length=121
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          610 a          728 c          649 g          640 t
ORIGIN      
        1 gacggcgaag aagtctatgt tgctccgacg gatgaagaat tcgacgcagc caacgcagca
       61 ttcgatgaac tttttgcaga ataatgattt agcaggtact gtcccggcgt aggtcttggg
      121 acagtttcct catttcttat ggcagtaagg atgacacagg gactcgcgcc ccggcaagat
      181 gcccggatgc gacgtccctt ttttagtggg ggagtaacat ggaattgcca tcgatacagc
      241 aattgcggaa ctttgtcatt tacagtaagt acggaaactt tacggctgct gccaaggcag
      301 ccaatatcac ccaatcggcc ttcagtgccc agatgaagaa actcgaagaa ctccttggcg
      361 tgcgccttat cgagcgctcc aaccgcggca gccatctcac gccggaaggg aaggacttct
      421 ataccaagat aagccatatc ctgggcgaac tggaggccgt cgtcagtgag cttcccggaa
      481 gggcagctac ggctcagcca ctggctgtcg gcatcatgct gtccttagga gacgtccaca
      541 tgaaccgcca tctggcctat tttcaggacc atcacagcgg cgcatccttc cgggtctata
      601 accttgaagt gcgggaaatg ctccaatggc tgaaggacga ccgcctggac atcgtgtcct
      661 tgttctatct gccgagcctt gacctcgacg attacgaaaa ggtcttcttt ggaaccgacg
      721 acatcgtcta ctacaaaccg gatctcgacg tcgccggtcc gtccatttcg gcctgtgccg
      781 tcgcggccga gcccttggct caatattcgc cgcagtacga gatgaatgcc tatatgggcc
      841 aatattttac ggctaacggc tgtccgcctc agaagacgaa agcttggttc tcgacgcctt
      901 atgccatgct gaactactgt cagcagaacc acatcggtgc cttgctgccc gaacggtttt
      961 tacgggccat gggtgtcggc aaagactact gccgtctcga tccgcccatc catttgccgt
     1021 gctatctggt ctacaaaaag gcgaatccca agtacgccgc gatccaagta tttgtcgact
     1081 acatccgccg cgtctatcac attgaaacgt gagtcataaa agatatttgt catcaaaaaa
     1141 aacgatgaca aatatctttt ttttctgtta tacaaggggc ggcggaaggt gtagaatcta
     1201 gcttgcaaat acgaaaggag cagtgactnn nnnnnnnngc cattgaactc tacggccgcc
     1261 gcatgtccct ttatggcggc atcatctttt atttcgtaac gaccgtattg tatttttata
     1321 tcccgaatct cgctatcctt tacgtcatcc gcttcttaaa cggcatggcc tacggcatcg
     1381 tatcgacggc gaccaacacc atcgtcgcct cctgtatccc gcctgaaaaa agaggccagg
     1441 gcatcaacta ctacggcctc agtaccagct tggcagccgc tgtcggcccc ttcctgggca
     1501 tgctccttat cgtcatcacc aactttacct tcatcattac cctctgcgcc gtcttggtcg
     1561 gcctgtgcat gatcggctgc atcatccttc atatcaatga aattgaactg acggccgaan
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 cctcggctat gggaccttca tgtcgaacgg acaggccgtc tgcatcaaat tgacgccgcc
     1801 gtaccgcatc agcgtggcct tgtcgacgta cttcgttgcc cttgacctcg gtctcggcgt
     1861 cgggccttac gtcctcggca gccttcaggg catcttgacc ttcccccagc tctacatcgt
     1921 cgccggtgtc atcgccgctg cctgttttgt tatgtattat ctcttctatg gacggaaaat
     1981 caacctcatc aatcgtgccg tccgtatcta tattcacgcc taggtgtatc atatatttca
     2041 ttttccccag actcagggac tgcgccgtac tttcggtgcg gtcccttttt tggaccttgg
     2101 ggcgtgtttg aagtttatcc ataagtcggt tataatggac gaaaggagaa ctcatgacta
     2161 caaaaacaaa gatactttcc ctattagcag ccgtctgcgt catcctcagc ctcttttttt
     2221 ggcctgaaaa agaaccggat gcctacagct cggcgacgat cgaagcggcc gaagaggtga
     2281 gccggaccga gacggaaatg atggcccccg atgaacgcct ctacggtccg aatggagccg
     2341 ttcgcctgca aagcttatat aatgaaaaac cggtctgtct ctttttctgg gccgtctgga
     2401 gcgacgacag cctgaagaac ctggccgtca tggaagagat gtacgaaaag tacggacagg
     2461 acatttcctt tgtcgccgtc cccgtcggac ccgatgccga tgagggggcg gaatactgcc
     2521 gaaaagaagg ctacgatctt cctgtctaca agggggatgg gcagcttttt cgggactacg
     2581 gcatcagcga cgtaccgcag gttatcttca tcgcccgtgg cggccaaatg agccggcctt
     2641 atgacggtct cgtgacccgt cgtcagattg aatatgcctt gaccaagatt caataaaaaa
     2701 agacttgcca agatgggaaa aacaaggtat actatatgag tcgcacgaaa tgtgcact
//
LOCUS       DAWDXZ010000212         2746 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7536_length_2746_cov_8.192493, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000212 DAWDXZ010000000
VERSION     DAWDXZ010000212.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7536_length_2746_cov_8.192493"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    307..399
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1319..1328
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2159..2168
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          546 a          728 c          737 g          622 t
ORIGIN      
        1 tatcccacat attggtatag gtcggcacga aaccttcttc gatgttgacg ccgaactggt
       61 tgcgcggcaa gtcgcgcagg tgtccgcggc tggccatgac cttatagccg ctgccgagga
      121 accgttcgat agtcttggcc ttggccggcg attcgacgat gaccaggttc ttagcattct
      181 taggaagctt gtcgagcttc ttcagaggct ctttcttggg aaggttgacc gtgccccgga
      241 tgacggtctt ttcaccgtcg cccttctttt tacgaccctt cttctttttt gaatcgtcta
      301 tgataannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cgcgaccttt caagcgtcgg
      421 tcccttatat acattataat ggcgtaatgt cttggtaaag aaatccgcag cttcaggccg
      481 aatcccccct cttttccacc cctttcgcca acatgctaaa aagacagcct tgcaaccgag
      541 tcgcaaagac tgcctttctg ttccttccta tggtatgata cggagaggat ggaaggaaaa
      601 attattttct tgtggttccg atgtgggcga ggatggcatc agtcatggtc ttcgtatcgg
      661 cttttttgaa gccctcttta tagaggtccg ccgtgcgcca gccctcttcg agagcagcgt
      721 cgacagcatc ttcgataagg gctgcccctt cttcttcacc aaaggagtaa cgcagcagca
      781 tggccgccga caagatggtg gcgcagggat tggcaatacc cttgcctgca atgtccggcg
      841 ccgaaccgtg gattggttcg tagaggctgg tcaattcccc gatggacgcc gacggcatca
      901 tgccgatgga accgccgagg acggccgctt cgtcactcaa gatgtcgccg aagaggttgc
      961 ccgtaacgat gacgtcgaac tgtttcggat tgagggccag ctgcatggcg cagttgtcga
     1021 cataaaaatg attcaaggcg accgacggat aatctttggc cacgtcgatc atggtgcgcc
     1081 gccacaggcg ggacgtagcc aggacgttgg ccttatcgac ggaggtcacc ttgccccgcc
     1141 gtttttgggc ggccttcata gcaaaggtgc cgatgcgcct aacttcaggg acgctgtaat
     1201 tttcaaggtc ccaggcccgt tcgacaccgt tatggatttc cgattcgcag cggtcaccaa
     1261 agtagatgcc gcccacgagt tcgcggacga tgaggacgtc ggtaccggtg acgatatcnn
     1321 nnnnnnnngg ccatctgtcg ttcaaaggtc aggcccaacc ggcagacctc gtctactttt
     1381 ttcagtaccc gcacggcgct ggccgtgatt tcttccccga tgccgtcgcc ggggatgacg
     1441 acgatgtgtt tactcatgag cctctgcccc cttgatataa ttgattaagc ctccggcagc
     1501 ggcaatgtcc tgaatgaagc ccggcagggg ccgtgcctga aaggtatctc ccgtcgtcac
     1561 atcgacgatt gatcccttgg caatatcgac ttcgacgacg tcgccggcct tgatggcctt
     1621 tacggcatcg ccgatttcaa ggaccggcag gccgacgttg atggcgttgc ggtaaaagat
     1681 gcgggcaaag ctggcggcga tgatgaccgg aatgcccgaa gctttaatgg caatgggagc
     1741 gtgttctcgc gacgagccgc agccaaagtt gtggccgccg accatgatgt cgccttgctg
     1801 cacctttccg gcaaaggttt cgtcgatgtc gaccatgcag tgggacgcca attctttcgg
     1861 atcaaaacta tttaaataac gggccggaat aatgacatcc gtatcaatat tatcgccgta
     1921 gcgccatact tttccttcta acaccatctt atcgtacctc cttgggagcg gcaatcctgc
     1981 cgagaatagc actggctgcc gcaacctgcg gacctgccag atagacttca ctgtcgacgt
     2041 ggcccatgcg gccgcggaaa ttccggttcg tcgtcgaaac gcagcgttca ccggcggcga
     2101 ggatgcccat gtaaccgcca agacagggcc cgcaggtcgg cgtcgataca gcgcagccnn
     2161 nnnnnnnngc ctgctgtaat gaacgtttca atatagcctt ttttcatggc ctccatgtag
     2221 actgactggc tgccgggaat gacaatgcag cggacgtcgg gatgaacgct gcggccttta
     2281 aagatttcgg cggcaatgcg caggtcctcg aggcggccgt tcgtgcagga gccgatgacg
     2341 acctggtcga tcttgatgtc accaaaggta ccgacgacct tggtattttc cggcagatgc
     2401 gggaaggcga cgacaggctg taatttcgac aagtcgatgt ctacggtccg gcagtacttg
     2461 gcgtcatcgt cggccgtaac gacttcgtaa gcgtcggcaa agcgcccttt ttcataggcc
     2521 agggtctgtt catcgacggg gaaaatgccg tttttggcgc ccccttcaat ggccatgtta
     2581 gaaatggtaa agcggtcggt catgtcgaga gaagctacac cgggtcccgt aaattctaag
     2641 gacttataga gagcgccgtc gacaccgatc ataccgatga gggtcagcat gacgtctttc
     2701 ccggtgatgt cttccggttt ttcgccggtc aggcggacct gaatgg
//
LOCUS       DAWDXZ010000213         2739 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7557_length_2739_cov_10.029806, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000213 DAWDXZ010000000
VERSION     DAWDXZ010000213.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7557_length_2739_cov_10.029806"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          632 a          783 c          655 g          669 t
ORIGIN      
        1 ttgccatata tgctcacctc cttattggaa taacgccgca aatgcagatg tatcatactc
       61 ggagatcgta tcgatctcct gcatgggaac tggcggcgtc cgttttttat ggaagcggaa
      121 aatgccggcc gcaaggtccg ttgttgggtt ttcctcttcc aggaattcga tgctaccgtc
      181 caggatatat tcgcgcgata caaggccatt cagccggatg cgctcagaat caagaatcgt
      241 cttgatatta cgcggcttgc ccggcttatc aaggcgactc caataggtta aaacaaaaac
      301 ggtatcatcc cagtcaaaca tgcgacgaac gcaaagaaat acgtctttgg ggtccgtcgt
      361 accaggatag gcgccggtat agttccccca cagtacccat cctgccggcg acgtaatcgc
      421 cgtaacaatg ccctgactgt taagcaggtt agcccgcgta aggtccagtg taacctcagt
      481 gccatccttg aggcataagc cggtaatcgg cagcgtcttg ttagacggcg actcatacgg
      541 tacatcgtca ttggcggcat ccgtctgacc gatgcggccc atcgcaagcg tagacatgta
      601 ataacaatga tcgccgatgc ggcccatcgg ccagccgacg aactcattat tttgcgtata
      661 cccgttgccg gttttccaca tgttgcagcc gctgtaggac ttaacctgtg tcgtgtcaat
      721 atccaccagt accgtacaac taaacaagct attaaatttc ttgcttttgg cggccatgac
      781 tgcggcgacc tccggatgat gactccagcc aggcgcagcc aaaagccccg gaacgagact
      841 ggtcttaggg tatacagtat cgagtaactc cagtcctgtt gctgtatcgt tactacctac
      901 accgccgata atgtcagcat ccttgaccat agacgggtcg atagtatcat aagcggcata
      961 gatagtcgtg ctatcggcaa gggcgccgtc cttaagcagt gtaataagta cgttgccatc
     1021 atcgttatat gctgccgtgt agtccgttcc tgccgtagcc gcggtaccag acgatgtact
     1081 ggatacctta agcgtgctaa ggataacgtc agcctcgatg acagccgtct tattagctac
     1141 cgtaactgcg gtactggcta ccgacttctt atgtttcgat gggtccagaa cgttgataaa
     1201 tacaatcggg gctaccgcaa aaagcttgaa ttctgcgtac atcgcctcgc agagcgtgta
     1261 cttatcccag tcggcatgat atcctaactg ctgtaccgct tccgtccagc tgtagcagag
     1321 gacgggctcg ttaattttcg ggtcactggc caagtgcacc ggcgccgtcc cgaataccac
     1381 aggaagcccg gcagttgtct gcgcagttgc aacgaccgcg gtatcgattt ccgacgtctt
     1441 gatgccgtga aaaaatgcca tgtctactta cctcctcttc tgtcaactgc cgcctggtag
     1501 aacgtattca gggcagttcc tttagttcgt atatctgcca atgcctgatt aagctcagac
     1561 ggcggcacaa acagatgttt tagaatttcg tcgtccgctt ccggcgacgg gattccgccc
     1621 gcgaaaatca tgcaatgatt cagccgtgag tcacggaatc cagggccaac atagataaca
     1681 ggcccagtgt atacagcagc tttttttgtg ggcacacgtt cttctgtgtc cacagctgcc
     1741 gttttttcct tatcctccat tttcgggacc tcctaacaga aacgggtcaa tgttatccgg
     1801 ctgcgggatg tggacctcaa aatcaatccg gccccaccat tgcggatacg gttgctcatc
     1861 cggtaccgac gtttccatcg tgccgttttt aacgtcgtac ttaccgcata ccggattatt
     1921 actcagcagg caatgccgga tgtattccag cacatggaac agctcatcgg cgccgtggat
     1981 catatccgag tcccgtgtaa caaccgatat aacgaccgat acgatagact catcggcccg
     2041 gtccatgacg cccgaatacc cgacaacgat tgccgggcat aaccgttctt taacctcagg
     2101 cgacatcgcc cgcggcaaaa aacctgtaaa acaattaatg gccttaccgt ctaccgtaag
     2161 gccactataa gattgcattt taaatttcag aaattttcgc aagtcattga gtacgtcgag
     2221 cgggaccatc taaccacctg ccaatcgttc cagctcatgc ataagccgtt tctcgtacat
     2281 cgccatgcct tcgtcggtca tcctcgctac gacggcagga ttaccgtaca actgtggcac
     2341 cgcaggcccg aacaggcctg ttactgggta tctgggccgt ccttcacggg ccgcaaaccc
     2401 cctcccaggc atatcgaagg accgcggaac aatagagccg ctgccctttt tgatcgagac
     2461 aaaaatacct ttccggcgac gcgttgcctt gtacttagtt accggctctt ccggacctct
     2521 gatgtggatg gtcgtcccta aggcgtccgg cttaataact gccgcgctct taaggtcgcc
     2581 cgccttaatg gtatacatac tccggatttc ctgagcccct gccttgcgtg ccgtaatagc
     2641 tgcgcggcgg gacgcctgct tgatagcact ctgcactttt ccaccgccgg caaagccctg
     2701 tagtacacgc tccaattgcc cggctcccat taagtcgat
//
LOCUS       DAWDXZ010000214         2739 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7558_length_2739_cov_5.682563, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000214 DAWDXZ010000000
VERSION     DAWDXZ010000214.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7558_length_2739_cov_5.682563"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1743..1752
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2388..2397
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          687 a          718 c          719 g          595 t
ORIGIN      
        1 aaaagaattg gttttactac tatacaatcg ttctgctcat cctcattatt ctcaatacat
       61 tcattatgcc gctgattcag cagcacagcg tgaaagaggt cgattacggc actttcatca
      121 gcatgactga gaagaaagag attggcaaag ttgaaattga aagcaatcag attgttttca
      181 cgaataagga cgaatcccag atttacaaga ccggtctgat ggatgatccg aaccgtacag
      241 aacgacttta tgaatccggt gcagaattct ccagcgagat tattgaacag atgtccccac
      301 tggtcagttt cctgctcaca tggattctcc cattggtcat tttctttgcc cttggccaat
      361 atatgtccaa gaagctcatg aacaggatgg gcggcggcaa agattccatg atgtttggta
      421 tgggcaagag caacgccaag atttatgtga aatccaccga aggcatcaaa ttctccgatg
      481 ttgccggtga agatgaagcc aaggaagaat tggctgaaat cgtcgaattc ctgcgcaacc
      541 ccagcaagta taatgccatt ggcgccaaga ttccgaaagg ggtccttctc tttggccctc
      601 cgggcaccgg gaaaaccctc ttggctcgtg ccgtagccgg tgaagccggt gtgcctttct
      661 tcagtatcag cgggtctgac ttcgtcgaaa tgttcgtcgg tgtcggtgca tcccgtgtcc
      721 gtgatttatt tacacaggcc aagaaaaatg ccccctgtat cgtcttcatc gacgaaatcg
      781 atgccgtcgg ccgccagcgc ggtgccggcc tcggaggcgg tcatgacgaa cgcgaacaga
      841 ctttgaatca gctgttggtc gaaatggatg gcttcggttc caacgaaggc attattacca
      901 ttgctgcgac gaaccgtccg gatatccttg acccggccct tcttcgtccc ggccgttttg
      961 accgtcagat taccgtcgac cgtcctgatt tacgcggccg tcgggctatc ctcgaagtcc
     1021 atgctaaagg taagccctta ggcaaggacg tcgatctcga catcatcgcc aagaagacac
     1081 cgggctttac cggtgccgac ctggggaacc tgctcaatga agcggcgctc cttgctgcca
     1141 gagccaataa gaaaatcatc aacatgccgg aactggaaga agccagtgaa aaggtaagct
     1201 tcgggccgga acggcgcagc cacgtcatca gcgataagga aaaacgcctg accgctgtcc
     1261 atgaatcggg gcacgccctc attgcctacc tcttgccgga cgctgacccg gtacacaagg
     1321 tcacgatcat tcctcgaggc cgtgccggcg ggtatacgat gatgctgccg gaagaagacc
     1381 ggtcgtatga aacgaaatcc tactacctgg cacagatccg cgtcgccctc ggcggccggg
     1441 ctgctgaaga aatcgtcttc aacgaaatca gcagcggtgc atcgggcgac ctccagagcg
     1501 ttacccgcat tgctcgccag atgatcaccc gcctgggcat gagtcctaaa ttgggcccca
     1561 tggtctttgg cgaacagcag gatcaggtct tccttggcaa gagtctcggc cacgaacgca
     1621 attacggcga cagtgtggct gaactgatcg acaaggaaat gcacgatatt gtatcggaag
     1681 cctatgccga tgtcctccat atgctgcacg aacgtctcga tacactgaat aagatggccg
     1741 acnnnnnnnn nngccgacgc cctcatagaa gtcgaaacga tcaaccgtca gcaagtcgat
     1801 aatttggtca aatacgggac cctcgaagcc cccgaagaaa aaacgaccga agccgtcgat
     1861 gcggaacctg ccgatacggc agcccagccg gcagaaactg tcgaaacaaa agccgccgat
     1921 gccgaaccga ctgcagaaga accggcttcg gaatcgaaag gtgaaacgcc tgtcgatgaa
     1981 gctcctcagc aggaacagac ggaagtccag atcagtcctt ggggcaaccc cgttcctaag
     2041 ggcggtctga cggatggcgt aatcgattcg gacgatgaaa agtctgacaa agatacgaat
     2101 aaataagttt atcatggctt agttcaaggc cgtttaaaga ctgccgtatg agctttatcc
     2161 tcatgcggca gtctttttgc gtgacgcctt gacaggcata tggggtatga tacaattttt
     2221 aaataaatga tgagcatgtt acggccgcta tacgatacct catgggcatg gtctgaaaag
     2281 cggcagtagg agggacgaat gaatatgcgg gaagatattt tcaacttttt acgaaaacac
     2341 caaaatgaat tcgtatcggg ccagcagatt tctgagcagc tgggcgtnnn nnnnnnnaag
     2401 ggctattgcc tgcgcgaagc accggaactc ttgtctccgg aagccctgga aaaaggcttg
     2461 gcttcgtccg aattcggaca gcatatcgtc tatcgggaac gggtcgattc gacgaataac
     2521 gtcgccaaac aactggccga cgacggagcg accgaaggga ctgtcgtcat tgccgaagaa
     2581 cagactggcg gccgcggccg tttaagccgt tcttttcact ctccctttgc caaggggttg
     2641 tggttctcgg tcatcctgcg gccggcgatt ccgccgatgg aagtctctaa gatgaccttg
     2701 ctggcagctg tcgccctggc ccgctctctg cgtgctcac
//
LOCUS       DAWDXZ010000215         2717 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7611_length_2717_cov_8.819684, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000215 DAWDXZ010000000
VERSION     DAWDXZ010000215.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7611_length_2717_cov_8.819684"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          556 a          752 c          750 g          659 t
ORIGIN      
        1 acggcgtcgt cttcattcgg ttccccgacc tggacgggct ggaaacgccg agccaaggcg
       61 gcatcttttt caaaatgctt cttatattcg tcagccgtcg tcgccccgat gcactgcaga
      121 tcgccgcgag ccagggccgg tttcaggata ttggcggcat ccatagcgcc ttctacggca
      181 ccggcgccga tgagctgatg gatttcatcg atgaagagga tgatcgtatc gtcgcgggaa
      241 atggtatcca agatgcgttt gatccgttct tcgaattcac cgcggtactt ggtaccggcg
      301 acgatgctgg cgaggtccag ggaaaagacg cgcttgtctt gcaggctgtc cgggacgttg
      361 ccggtaatga tacgctgtgc cagcccttcg gcaatggccg tcttaccgac gccgggagcg
      421 ccgatgagga ccggattgtt cttcgtccgt cggcttaaaa tctgaataac gcggctgatt
      481 tcttgttctc ggccgatgac cggatcgacc ttatcctttt tggcccattc attgaggtcg
      541 cggccgaact gggacaaatc gctgcccttt ttatccccgg caggctcact gccggctgcg
      601 tcgccatcgt tcatgagctc gctcaagcgc tgggacacga ggtcgggatc gatatccata
      661 ttttcgagga tctggacagc catgcccgtt ccttcggcca ggatgccgaa caagaggtgt
      721 tcggttccga cgtagttctg ttccagccga ttggcttcac ggacggccat ttccatgacc
      781 cgtttagtgc gcggcgtcag gcgcagtttt cccgtcgtat cggtccccca accgacctgc
      841 tgttcaatgg cacgggtaac attctctgac gtcaggccca gctgagagag gatcagtccg
      901 gcaatgccgt ctttatttaa aacgagaccg agcaggatat gctctgttcc gacgtagtca
      961 tgatgaaatt tctgtgccgt atcctgtgca aactgaagca cggctttggc atcgttggta
     1021 aatctgttgt tcgccatggg aaatcacttc cttctgtact cacggagcac ttgctgcacc
     1081 cttttggcgc gttggtgctc taagtcttcg ccgcttaatt ccgatcctgc ctgaagttgg
     1141 atccaggcag gatccgaagc ggccataatg gcttcataag ccaggggctg cacggggatg
     1201 acccccatgt cgatgccgaa gcgaagatcg ctggcaaagt tcagcgtttc ccggtcatcg
     1261 aggagccagg cctgactgag ggtgccccag gtgcgcagcc atttatcttt caagttattc
     1321 ttattgtgat cccaaaccaa ggtgcggcag ttctgttctt cctgcacgat ctgggtcacg
     1381 atttttcgca gttgttccat cgtatcggct tcagtcaccc ccagggtaat ctggttggtg
     1441 atctggaaaa tattgccgat ggagtcgttc cgttccccgt agataccgga gaaggtaaat
     1501 ccaaatttcg tcgtcccttg gacgatgcgg ttaaaacgct tcataaggac taaggccggg
     1561 acgtggaggg tcacgccgac ggtcaggccc gtaccggtca gggagggact tgccgtaagg
     1621 tagccgaaat catcacggaa agcaaaatcc aaatggcttt ccaaggcatc gtcgatctgg
     1681 gcggcctgat cccaaacgcg ccgcaggtca aagcccgggg cggcaacttg gatacagaaa
     1741 tggtcgtctt cattgaccat gatcgacacg gcaccgtcgt cgcggaggag aatcccccga
     1801 tggttcggcc gttcaatatg gccggctgac gacagatgct tgacggccag tacctcccgt
     1861 tcgagaggcg acaagtcttc aaaagaaatg aagcggtaat ggccctgtcc cagggtgttc
     1921 agtgtaccga gctgtccttt ggcttcgtcg aggacggccg acagttctgt atccgaggct
     1981 ttatcgggaa atacatattt ctttaaattc cgattcaagc ggacccgact gtcgaggacg
     2041 atgtcccgga attcaccggt cccgtcctgc caaggacaga gcggcgccat gagcatatcg
     2101 cgtaccacgg cttattcccc ctttccatca gccggaagct ggcgttggag cgcccggatt
     2161 tcgtcgcgga ccttggccgc ttcttcgtag cgctcttcct gcacgagctt ttgcaggtgc
     2221 tggcgcaggc gcttaacctg cgttgccgtc cgaaagacgc cggtcccgcg attcggcacc
     2281 ttgccgacgt gcttgctgtg gccgtgaatg cgccgcagga gaggcggaat ttcttcctta
     2341 aaagcttcat aacaatcggg gcagccgaat tttcccacat ggttaaactc cccgtaggtc
     2401 atgccgcagc tggcacagcg cggttcatcg ccgctagccc cttggtaatc gggataggcc
     2461 atcttacgga aaaaatcatt atcgataata ttgacatctc gcatattcga aaagtttgac
     2521 tgtcttttag cgcaggtagc acacaggtgg acgtccgtac gctgcccgtc ttcaattttg
     2581 acgatatgga caacagcctc gcgtttttta caggcatcac agagcatggc cctcacgtcc
     2641 tttctatctt caatgaaggt tcagattttt caaaatgtcg ctgatgacga gtaagcgtga
     2701 agcctcatca tcgatgc
//
LOCUS       DAWDXZ010000216         2717 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7612_length_2717_cov_8.712622, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000216 DAWDXZ010000000
VERSION     DAWDXZ010000216.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7612_length_2717_cov_8.712622"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          703 a          709 c          668 g          637 t
ORIGIN      
        1 gctcgacgcc tccgtcccct gcacggtcac catggaaaag acgccggacg atacctggcg
       61 gattacaggc cttgccgatg ccaagggctt tatcagtgac ctgcaagccg tcatgaacca
      121 tgaactcgac gcctacaata gaccggtccg gcaaaaaata gacgccctat tgacccttca
      181 aaatgtatca gccaaactcg tatccgacca ggacaaaacc tttctccgcc tgagttatac
      241 cccggtcttt catcaggacc gagaacagat cgctgccatc aaagccgtct ataccctgcg
      301 ccgcaagagc gatgacgccg tcctctatac gtcgcccctg cgactgtcaa cgtcggctga
      361 aaggagcacc tatgaaagtc agtttctcct caatcccttg attccttcgc agtatgctct
      421 catccgttcc gccaatatcg acgatacgac gagcatcctg gaaatcacgg ccatcaccct
      481 caaagacggg acggagctgg ccgtcgaaaa tgacttaccg caaagctact gaatgacaac
      541 cgatatacaa aaaaatcgcc cgccgtggaa aaaagatgtt tccatcggtc gggcgatttt
      601 cagtatgacc ttaatagcag atttcaataa ttttcggctt agacgattct tcttttgaca
      661 gcgagcggtc gaagagtttt acgccgacag cgccgatcat gaaaccgatg acaccgccga
      721 tgacagcggt ctgaacggta tcaaagcctt gggacaaggc caggaaatga ccggcaaaag
      781 caccggcagc acagacgagc agcggcatga caaaacagat aaacgcaccg atgacgatat
      841 tgacatcgcg gaattcaaag cgaacgtgct gccccacttt gacacctaac ggatcgacgg
      901 ccgtaacgac aatattgtca gccccgtcac aagcgccgca ggcgatgcag tcggaatggc
      961 ggccaacttt gatttcggca cttccggcct cttttatagc gataatggtc ccttcttcaa
     1021 tcttcataaa atccccttct ttctgacctt attatagcat gggttatttt ttcaatcaat
     1081 actattttcg tgtacattaa atagaaaata ggaatacaaa aagcgactgc caacctccta
     1141 aggagaaggt cagcagccgt ttggatcaat gattttctgc cgtttctaag aggaacttcc
     1201 ggaagggttc tgctaaatca ggacgacgaa gggcatattc gacattggcc ttcaaaaagc
     1261 ccagtttatc gcccacatcg tagcgctttc cttcaaaaca gtaggcatag acagccttct
     1321 ggcaggccag ggtctgcaag gcgtcggtga gctggatttc tccgcccttc ccgggcttcg
     1381 tgtcccggag gatatcgaaa atatccggcg ttacgatgta gcggcctaag acagccgtcc
     1441 ggctcggcgc ttcttccaca gccggctttt caatgacctg ctgaactttc agcaccggtt
     1501 ttccctcgac gggaatccct tggacgatac cgtaggacga aaccttttgc aggggaactt
     1561 cctggcatcc taaaattgtc gagtcgagtt cattgtaaag atcaatcatc tgccgcaagg
     1621 ccgggttggt atcgttatag acgacatcat caccgagaag gatggcaaag ggttcatcgg
     1681 caatgaacga ttgggcacag cgaatggcat cgcctaatcc gcgcatgtgt ttctgccgga
     1741 tatagtggat attgatttcc gaaatactgc gcaccatgtt gagcagtttc gtttttcgac
     1801 cttcttcgag gtgcatttcc aagcccggat tggtatcgaa gtggtcctca atggcccttt
     1861 tggcatggcc gctgataatg aggatttctt caataccgct ctgcagggct tcttccacga
     1921 tgtactggat cgtcggttta tcgacgatgg gcagcatttc ttttggtgtc gccttcgtgg
     1981 ccggcaagaa acgggtacca aaaccggcgg cggggatgac ggctttacga atctttttca
     2041 cgctaaaact ccttattcaa accaacgaga caagtcaaat tcttcgccaa tgatttcttt
     2101 gacgaatcct tgggccaagt agagacctaa cagggacaag gtcgatacgc gcagaagcga
     2161 gctgtcccaa atatcaccga ggtcagccag gaccggcgaa atgtcttcca tgatatccag
     2221 ggcttcttcg ccgctgaaat tagccgggcg ctttttagcc aggcgcagga tatgatcctg
     2281 aatattcctg tcggtgaggc cggtcatgcg gaaaaaccgc ttggccatac tttcatcgac
     2341 catagccagt ttgactaagg gcgaattaaa ggactgaacg atgaactcgt cggaaaggcg
     2401 catgcccttt aaggccttgc gcagttcttc ttcggtaaaa ccgcggtagc ggaacagaag
     2461 cggataggaa tcggacagga tttctgcaaa gtgagtttcc ttcacttcaa aggccgtgca
     2521 gtgcaggtag tcgagctgct gataatacgg ccgttccgtc gggaatcctt ccaggaaggg
     2581 atagatatac ttatcaatgt actggcagaa cgagtctttg tcgacattgt gagagtttcg
     2641 cgaatacagg aacaataaga ggatcgccaa aatctggtaa tggctctgag acaggtaagg
     2701 catgaccttc agggcat
//
LOCUS       DAWDXZ010000217         2715 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7619_length_2715_cov_8.791353, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000217 DAWDXZ010000000
VERSION     DAWDXZ010000217.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7619_length_2715_cov_8.791353"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1728..1737
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          557 a          719 c          756 g          673 t
ORIGIN      
        1 gacggaatgc agccgacgtt gatgcaggta ccgccgtaca tgccgttgtc tttttcgatc
       61 aaggcaactt tcttgccttg tttgcctaat tgtccggcta aggttttacc ggctttccca
      121 aaaccaatta cgataacatc atacattttc ataattaagc ctccttaatt aaaaatatat
      181 aatcgttggt atccatttat gacagcagtc cggcaaagtc tcccctgccg gccgttgttt
      241 ccttgaaaaa gattttagac gccggtgctt ccgatgccgc ccgtacgaag gccgccggca
      301 gtatcgtcgt tgacggtcag gtacttcatg aagatgccct gaccgatgcg gtcgcctttt
      361 tcaatcgtat aggccacatc gttcgtattg tagtaggcga tcataatatg gccttcatta
      421 tcttcgttat tatagtagtc gctgtcgacg atgcccgtgc tgttggccag catgaggccc
      481 tttttaaagg ccaggctgga gcggatgaaa atggacaagt attcatcgcc ttccatgaaa
      541 gccttgaggc ccgtcgggac gacggtcacg gcatgaggcg ccagcaccgt cgtttcagcg
      601 gcttccaaat cgtagccggc actcttagcc gtcttgcgaa tgggaatatg gatgtcacaa
      661 tctttgtagg ccgtgacgat ttcaaaacct ctgcccgtca tgacggccta ccgtttctgg
      721 cggtcgcgat tgccgctgtg accgccgcgg ggaccgcgac ttctgtggga cggagcttcg
      781 tcaccgccgc cgaggagggc tttacgggac aggttgacac ggccctggct gtcgatttcg
      841 acgcacttga ccatgatttc atcgccgacg ttgactacgt cttcgacttt ttcaacgcgt
      901 tccatggcca gctgggaaat atggaccaag ccttctttcc cgggcaggac ttcgacgaag
      961 gcgccgaagt tcatgaggcg agtgacttta ccgacgtagg tcttgccgac ttctacgtct
     1021 tccgtcaatt tttcgatcca agctttcgcc ttgtcaccac tttcgccgtc gacggctgcg
     1081 atgtagacca ggcccgaatc gtcgatgtcg attttagcgc ccgtttcttc ggtgatctgc
     1141 ttgatcatct tgccgcccgg cccgatgacg tcgcggatct tatcggggtt gatgtgcatg
     1201 gaaatgatgc gcggagcgta gggagacagt tccttccgcg gttcatcgat ggcctgcaac
     1261 atcttgctca tgatgtggag acggccttcg tgagcctgtt tcaaagcctg atcgaggatt
     1321 tcacggctga ggcctttgat cttgatatcc atctgaatgg ccgtaacgcc tttcatcgta
     1381 ccggcaactt taaagtccat atcgccgaga gcatcttcca tgccctggat gtcggtgagg
     1441 atggtgaagt cttcgccttt cgtgaccaag cccatggcga cgccggctac gggagcctta
     1501 atgggaacac cggcatccat cagggacaag gtgctgccgc aaacgctgcc catagagctc
     1561 gaaccgttcg attcgaggac ttccgatacg acgcggatcg catacgggaa ttcttcttca
     1621 ctcggcatga cctgagtcaa ggcccgttca gccaatttgc cgtgaccgat ttcacgacgg
     1681 ccgggaccgc gggaggactt cgtttcgccg acgcagaagg acgggaannn nnnnnnngca
     1741 tgtagcggcg gtccgtttcc aggccgacac cgtcgatgtg ctggctttcc gacaagggag
     1801 ccagggtgca gaagctcatg atctgcgtct ggccgcgggt aaagaggcct gtgccgtggg
     1861 tgcgggcaaa gagaccgact tcacagctga ccggacggac ttcgtcgacg cggcggccgt
     1921 cgggacgaat cttatcgaca gcgatcatgt ggcggacgat ttccttgatc atcttctgag
     1981 taatgtcggc gacgtcgtcg ccattatcag ggtagatgtc ggcaaagtgt tcgtagatgt
     2041 cggcttcgac ggtgttcatc ttttcttcac gttcgatctt atcggccgtg aagatagccg
     2101 tcttcatggc ttcttcgccg taagcgtgaa cggcttcgac gatgtcagcc ggcggttctt
     2161 tttcgacgaa ttcctgtttc ggtacagaaa tgctgtccag gaaggaaagc tggaattcga
     2221 cgactttctt gatttcttca tgggcgacca tgatggcatc gagaatctgt tcttccggaa
     2281 cttctttagc gccgccttca accatgagga tggcgtcttt cgtaccggca acgacgatgt
     2341 ccatgtcact ctgttcgctc tgagcgaccg tcggattgat gacgaattca ccgtcaacgc
     2401 ggccgacgcg gacaccggcg atcgggccgg caaagggaat atgagacatg cacagggaaa
     2461 cagaagcgcc gatcatagcg gcgatttccg gggcattatc gtgatctacg cagaaatcat
     2521 aagccacgac gtggacgtca tgacgatacg ttttcgggaa gagcggacgg atcgggcggt
     2581 cgatgagacg ggcaaagaga gtcgccgatt cgggcggttt gccttctcgt ttaataaagc
     2641 cgccggggat tttgccgaca gagtacatct tttcttcgta atcgacagtg agcgggaaaa
     2701 agtctgcgtc ttcac
//
LOCUS       DAWDXZ010000218         2704 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7639_length_2704_cov_7.531521, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000218 DAWDXZ010000000
VERSION     DAWDXZ010000218.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7639_length_2704_cov_7.531521"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    223..232
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          661 a          674 c          737 g          622 t
ORIGIN      
        1 gattagaatg gaacgaggga caggcgagaa ataaaggctt tttgaagcag gctcatggga
       61 gcctgctttt tttatttgaa agggaggaga cacgacaatg attattgtgt tgaaagcaaa
      121 tgcgacaaaa gaagaagtaa aaccattgat tgacagcctc aaagccaagg ggatgcagat
      181 tgacatcagt aaaggtaccc gccagaccgt catgggcctt gtnnnnnnnn nnccggtgtc
      241 atcgatgtcg aaagggtgca aagctatgaa tttgttgata aagtcatgcg tgtcgaagtc
      301 ccttacaaac gagccagccg tgcctttcat cctcaggatt cggtcattcc tgtcggcagc
      361 agcggtgttt ctatcggcgg taaaaaatta gccgtcattg ccggtccctg ctccattgaa
      421 acaccggagc agatcgaagg cgtcgcctgt gacgttgcaa aatccggggc agctgttctt
      481 cgcggcggag cttttaaacc gcggacgtcg ccgtactcct tccaaggact cggaaataag
      541 gggctcgata tgctgcgtga ggccggccgc aaggcaaaac ttccggtcat cacggaaatc
      601 atgtcggccg ataaattacc ggtctttctc gaagacggcg tcgatatcat ccaaatcggg
      661 gcccgcaaca tgcagaactt cgacctcctt cgggaggtcg gcaagacgag aaagcctgtc
      721 ttattaaaaa gaggcctcag cgcgaccatc gaagaatggc tcatgtcggc ggaatacatc
      781 atggctgaag gcaaccgcaa cgttatcctc tgtgaacgcg gcatccggac ctttgagact
      841 tatacccgta ataccctgga tttaagtgct gtccttgccg ttaaaaggca aagccattta
      901 cccattgtcg tcgatcccag tcatgcgacg ggaaaacgct ggatggttgc tgatttagcc
      961 agagccgccg tggctgccgg tgccgatggg ctgatgattg aagtacataa taatcccgat
     1021 aaggcgttgt gcgacggtgc gcagtcgatt acaccggcca tgtttacgga gctcatggat
     1081 tcacttcgta aattagcgcc cattgtcgga cgagaattat aaggaaagct gggtgtgtaa
     1141 tggatacgga agctagtggt tttaatacga acgagacggt agctatcatc ggcttagggc
     1201 tcatgggcgg ttctttcgcc cgcggcctgc ggcgcatcgg cgtaaagcat atcatcgccg
     1261 tcgatcccga tgagaaggcc ctccaaaagg ctaaacagga agggatggtc gacgaagtct
     1321 atacgacggg cagcgtcgct ttgaaacggg ctaagatgat tattttctgc acggcgacgg
     1381 cggtcatgat tcgatttttg gcggaacata agaccttctt tgcaccgggg accatcgtaa
     1441 ctgacgtagc cggcatcaag ggcggtacgg ccgatgcgat taaggcgctt ctcccggatg
     1501 gcgtcgactt catccccggt catcccatgg ccggtcggga agggaagggc tatgccatgg
     1561 ccagtgccga tatttttaac aatgcgaact atatcctcgt accgatgccg gaaaataagg
     1621 aaaatcacat cctggccgtt accagtatgg ccatggctct gggctgtgcc catgtcgttc
     1681 gcgtatcgcc ggaaaaacac gatcgcttca ttgcttatac cagctgcctg ccccatgtct
     1741 tggcgacggc tctggtcaat agcgaatcga tggatacgat gaccaaatac ttcgtggccg
     1801 gcagttttcg agacagcacc cgcgtagccg acatcaatgc cgctttgtgg accagcctgt
     1861 tcttagccaa taaagagaat ctcgtccatg aaatcgattg cttccgtaag gccttacaag
     1921 attttgcaga catgctctct gatgaagatg ccgaagggat gacccgattt ttacagcaag
     1981 ccgctgcgcg gagaagggac ttagtttatg aaacacattc acgttgaatt aggtaaagat
     2041 tcgtatgata tccatattga aaatggactg cttgaccggg ccggcgacta catccgcagc
     2101 ctgaccaagt cgcagacctt agccgtcatt accgactccc atgtcgatgc cctctatgga
     2161 ccgcggttgg aggattctct gaagtcggca ggatttcggg ttcatcgact ggtcttcccg
     2221 gccggagaag aacataaatg tgcagaatcc cttctcgcct tataccagtc cctgtccgat
     2281 tcgggaatta cccgcagcga ctacgacggc atgggtgaag tcattaaata cggctgtatc
     2341 cgcgatttgg agctttttga actgttagaa aacctcgatg atgaagctat ttttgatcat
     2401 tgggaagaaa tcattgagcg ctgctgtaag agtaaagccg atctcgtcga acgagatgtc
     2461 ctcgacatgg gggaacgcat gattttaaac ttcggacaca ccatcggcca tgccatcgaa
     2521 ggctgctacg gctttggctg ttatacgcac ggtgaaggtg ttgctgccgg catgtcgatg
     2581 ctgaccggcg tcaccgaggc gatgggcctt acgaaagaag gcacggcgat gcggcttcga
     2641 aaacttctca gccgctacgg cctgcccgtc gacgtcgatg cctcggcagc cgacctcatc
     2701 ccct
//
LOCUS       DAWDXZ010000219         2703 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7643_length_2703_cov_7.645770, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000219 DAWDXZ010000000
VERSION     DAWDXZ010000219.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7643_length_2703_cov_7.645770"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1116..1125
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          544 a          741 c          787 g          621 t
ORIGIN      
        1 tgaaagggga aggtatcatg gaaacaaagc ttttaggcta ttatggagaa caggcggccc
       61 tggcttatat acggcatcag acggattatc gcatccgctg ctgcaactac cgcaatcgca
      121 tgggtgaaat cgatattatt gccgaccatg gacggaccct tgtttttatc gaagtcaaga
      181 cgcgaagatc cgagcgttac ggcaggcctt gtgaggccgt cgaatggcgg aagcggcaga
      241 aaatcacgtc cgtcgcctca ggctatctga agcagttccg actatgggat aagccctgtc
      301 gctttgacgt catcgaagtc tggccgggag tcgacgatca gcctgtcatc cgtcacgtcg
      361 tccatgcctt tttggcggaa aggcggtgat ttcatgtttg cacgtatttc aggggcggcg
      421 accttaggtc tcggcggcca cgtcatcgcc gtcgaaacgg atatatccaa cggcttgccg
      481 tcttttgatt tggtaggttt ggcctcgacg tcggtcaaag aagccaagga acgagtccgg
      541 gcagccatta agaacagcgg ctatgagttt cccatgcgcc gcctgacggt caacttggca
      601 ccggccgatt tgaagaagga tacgtccggc ctcgatttgg gattggctgt ggctatcttg
      661 gcctcgagcg gccagattcc gctggaatcc tgcacggact gcctcttttt aggagaattg
      721 gctctcgatg gagagatacg gcccataaac ggcgtcctgc ccatggttat cgaaggggct
      781 gctcaaggcg cgaagtccgt ttttgtgagt ccgaaaaatg gcgccgaagc cctcctttgt
      841 ccgtccgtta cggtctatac ggccgagtcc ttgtcctgtg ttgccggtca tttgaagggc
      901 gaggcgcctt taaagcccat tacggctcaa gaacggttgt ccgatatcct cgattacgac
      961 ctcgattttt ccgaagtaca aggtcaggtc gaagccaaac gggcccttga aattgcggcc
     1021 gccggtggtc acaatatttt aatgatcggt ccgcccggaa gcggtaagac catgctggcc
     1081 aaacggattc caacgatttt gccgcccttg gggaannnnn nnnnngggca gctcatgcaa
     1141 aggcgtccct ttagaagtcc tcaccatacc atctcccggg ccggacttgt cggcggcgga
     1201 acgattcctc atcccggcga agtgaccctc agtcatcatg gcgtcctctt tctcgacgaa
     1261 ttgccggagt ttccccggtc ggtactggaa gtattgcgtc agcccctgga agacggcatc
     1321 gtccacatcg ccagagtcaa tgctgcctta tcctatcctt cacggttcgt cctggtggcg
     1381 gccatgaatc cctgtccctg tggccgctat ggatttgacg acgattgtac ttgttctgaa
     1441 aacaaccgcc atcgctatac ccataagatt tcaggtccct tgctggaccg tatcgatctc
     1501 cacgttcgcg tagcccggcc ggcttacggt gatctggtag cgagcagtgg agccgagacg
     1561 tcggcgacga tacgggaccg ggtccttctg gctcgggagc ggcaagcggc acggctgtcg
     1621 ccttatgact gttattgcaa tgcccatatg gggcaccgag aaattaagga gacgtgctgc
     1681 ttgagcccgc cggctcgcga tatgctgaaa gaggcttttg accttcttcg cctcagcgct
     1741 cgcagctacg accggctcat caaggtggcc cagactattg ctgacctgtc cggcgccgag
     1801 gagattgaag aacatcatgt agccgaagcc atcagctacc gcaataaact gtaacggagg
     1861 tgacgctgtg cgtggcatca cggaagaaac gatgtatgca gcggccctgc aatcccttca
     1921 cggtctcggc agccggcgct tgaagacgat cctcgaccgt tatcgttcgg ctaaagctgc
     1981 ctggaatgcg ccgtgggacg acgagctgcg gcgtcggctt cgattttcgt cagcttcttt
     2041 tggtaaactt attgaagaac gtaacgtttt tgattgggac gggttttccc gcaaactttc
     2101 tttttatggc gtccggccca ttgccttgtg ggacgacgac tatccctgct ggctgccctt
     2161 cattgcccag ccgccgttgg tcctcttttg tcagggaacg ctggcagccg atcggtatac
     2221 cttggccatg gtcggttctc gaaaggccag cccctacggg ctgaacgctg ctgaaacctt
     2281 ggccatgagt ctggctgaac gggggattac cttggtcagc ggaggggccc ggggcatcga
     2341 tacgcgaatt catacgggag ctttgaaagg gaaggggcga acgatcgtcg tcgtggccaa
     2401 cggcctcgat cggacctatc cgcgggaaaa taagcggcta tttcgagagg ttgttgaaaa
     2461 tggcggggcc atcctttcgg aatatacctt cgggaccgag ccgcgggccc agcattttcc
     2521 cgcccgcaat cggattatcg ccggcatggc ggcggccacc gtcatcatcg aagcggccct
     2581 tggcagcggc tccctcatta cagccgactt tgccttagaa gaggggcgcg atgtctttgc
     2641 catgcccggc agtatttttg ccgagaccag taaggggacg aatcgcctgc tgcagatggg
     2701 ggc
//
LOCUS       DAWDXZ010000220         2693 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7660_length_2693_cov_8.671342, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000220 DAWDXZ010000000
VERSION     DAWDXZ010000220.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7660_length_2693_cov_8.671342"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1503..1512
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1984..2005
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          668 a          727 c          707 g          559 t
ORIGIN      
        1 aagaaatcaa atatatccgc cacaggttct gcgttatacg cacaaggacc gccggcggcc
       61 actaagggca tatcgtcgcc gcggtctttg gaaaaaatcg gcattccggc cagatcgagc
      121 atatttaaga tgttggtaaa actcatttca tattgaaggg taaagccgac catgtcgaaa
      181 tcgcggacgg gcgtcttcgt ttccaacgaa aaaagcgggt agcctttggt ccgcatgagg
      241 tcttccatat ccggccaagg cgcatagacc ctctcggcag ccacatcagg ccgttcgttg
      301 agcagggcat agaggatgcg caggcctaag tggctcatgg ccacttcata cacgtcggga
      361 aaacagtaag caacggtcag tttgacccgg tcgtggtctt ttacgacgct gttccattcc
      421 ccgccgacgt agcgggccgg tttactgact ttgctcaata aaatcggatc tatctccaca
      481 ggatgtgaca attcatttcc tccttcagga atcgctaaaa gatcatcgtc tgctgacgca
      541 tgtaaatact caataaaata cccatgccca tcatattcgt cgtcaaggcg ctgacgccgt
      601 aactcatgaa gggcagcgga atccccgtga ccggcataat accgcaggtc atgccgacat
      661 tgaccaggat ctggaaggcc cacatggata cgacgccggt ggccagcatc atgccgaagg
      721 tgtccttaca ttccttggca atcatgatac ctcggtagat gaggataaaa tagaggaaca
      781 agagaaaaat acagcctaaa aatcccagtt cttccccgat aacggaaaag atgaagtctg
      841 tgtgattttc cggcaaaaag ttcaattggc tctgcgtccc ctggaacaag cccttgccaa
      901 agagcatgcc cgaaccgatg gcgatcttgg actggatgat gtggtagccg gccccgaaag
      961 gatcggcgtt agggttgagg aataccgtaa tacgagactt ttggtagtcc ttcagaaagt
     1021 gccagcccac cggtgccagg acgatgccgg ccaaaaagac gttgcgcaaa agagacatct
     1081 tgatgcggcc gacaaagagc atgcctaaag taatggccaa aaagacgaga ctggtcccca
     1141 ggtcaggctg tttcaagacc aagaggaagg gaatcccgac gtaaaggccg atgggaagca
     1201 cctgccgcca cttattgagc tggccgacgc ggtcgctgag aatggctgcc gtgcagatga
     1261 tcatgatgag tttcgaaaat tctgacggct gcagggtaat cggccctaac tggatccagc
     1321 gctgggcccc gagagccgac gtaccgacga acatgaccgc caccagcatc agtaagttaa
     1381 tgacatataa gggtttgccg tacttcttta acttggtgta gtcaaagcgg ctgaaaaaga
     1441 tgacgatcat gaaatcgacg acaaaggcta cgccttgttt aatgacgaaa tcatagttga
     1501 tcnnnnnnnn nngatatgcg tcgcactgcc gataatagcc aggctgatgc agacgatgaa
     1561 aaagctcacc aggagcataa ctttatctaa attcttccat tcacgtttaa taaacatttc
     1621 cctctcctat cgacgatgat ggcgccaatt ccggcattcc tgccggcggc gccgcaccga
     1681 taagcctgat tcggcagcgt ctccgccaga gcgccgcggc agcaggcttg tcagcccggc
     1741 ttcatcaata gccgtcccct gttcaagcca gtccgccgta gccgacaagg ccttcatgaa
     1801 aggcaggctg tctgaggctt cgtgaatcag gccctgactg gccgcctgat cgatgccctc
     1861 gtcgtgaggc aggatcccgg cgacggtctt tacgccaagc gtcgatttcg ccgaggctgc
     1921 cgaaatgaca ccgtcggcgt aaaaattatt gagaatgacg tcgtagtgaa aacgtttctg
     1981 tttnnnnnnn nnnnnnnnnn nnnnncttct gactggccgg caggacgtcg aggccgggac
     2041 agaccgacac cagggcctgt cggggcagac attttccccg ggcagcctga ctggcgtcgt
     2101 aacggacggc atcgcctacg ccaaaaagca aatcgaggtt gcgcagtccc atgtccgcat
     2161 cggcagccag cacccgatag ccgcggcgct ggagcagtac ggaaagagaa gctgtaatca
     2221 aagttttgcc gacgcctccc ttaccagaag caatcgcgat gagtcgagac atggaattac
     2281 cacccttctt aaagtaccat tagttattgt tcgggtcagc cgtctggccg ctgctgttgg
     2341 cctgattcga cgctgccggc gtgccgcttg tattcttcgc tccgtcggca taagtaccga
     2401 tgtggaagaa ttcttccagc atggaacgga caatcgggcc tgccgaaacg gacccgaacc
     2461 cgccttgttc taccaatacg gcaatgacga tacgcggatg ttcatacggc gcataggcga
     2521 cgaaccagcc gtggtccgta ccgccgaagt tttctgccgt ccccgtttta ccggcgacct
     2581 ggatagggaa accggcaaac aaggagcctg ctgtccctga ttcttccgca acgttgcgca
     2641 tcccttcgcg gacaaggtcc agcgtcgatt tggaaaccga cagggtccct atc
//
LOCUS       DAWDXZ010000221         2689 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7674_length_2689_cov_7.471906, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000221 DAWDXZ010000000
VERSION     DAWDXZ010000221.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7674_length_2689_cov_7.471906"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    644..672
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2293..2302
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          664 a          576 c          739 g          671 t
ORIGIN      
        1 gcttttggaa gagctgctgc ttgacagtct tataacgagc catagtcgtc ttgatggacg
       61 attgtgcaag ttgcgacttt agcccaaacc gttcgcggag attgcggtac agcgcttggt
      121 taaggttttg gtaagccaag ttgaattgat tgtcgaagat gtactgcgac acaaaattac
      181 acgcctgacg ataatgctct gtcatttggc gaaacagaat ctcttgttcc ggagaaatat
      241 ggatgcgaag ttttatagtc ttggttaagt cgggcacttt tttctcacct gcctttctga
      301 aaatatatgg cttcccttaa gaccaccata tcatatatgt tactaaaact ctagtgtttt
      361 aagaaagtga ggtgaagcgg gcattcctcc ccgacctaag aggtcggagt atcctgcccg
      421 catttagatg aattttggga gctgcagtca gaaatgggga cactgtataa gttatcaaaa
      481 atccgacttc actatcgttg acagtgttag tacgactcga cagatgagga taaaaggaaa
      541 tggtaggggg cttgcctgat ggtattgacg aagaatcctg catgcgtaga gaaaaccttg
      601 attgaggtac tgacgaagat ttatgacgcc gtgagctttc aagnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnctatattg atttcctccg gctgcatggt tatacttttt ttaggaggga
      721 tggatatgga acttcgggta ttgagttatt ttctggccat tgccagagag gaaaatttta
      781 cacgggcggc ggctcagctc catgttaccc agccgacctt atcccggcag attgctgatt
      841 tggaagccga gcttggcgtg aagttgttta cccgcagcaa ccataacgtg gtgctgacgg
      901 aagacggcat gctgctaaaa cggcgggccc gggacatctt gacccttgcc gagaagacga
      961 aacgagactt tctccgaaag gaaacacttt tggaagggac gattaccatc ggcagcggtg
     1021 gtcatgggga gaacctgaaa gacgggattg atcgggggct tttggatatg gggcttatgg
     1081 ccgaacccct tgatattcaa aagtacgaat tcgtgtccat gccggtaaag gaggagtggg
     1141 gcgccttggt ccgggaagat tctcctttcc tgtctaaaag cgtcatcacg cctcaggatt
     1201 tggtggggat tccccttatt tctgccgtcg gcaacatcgc cgaaagtaat cttgggaaat
     1261 ggttcggacc ggagctgagc aaggcgaatg tcgtcgccaa aggggacctc ctgtacaatg
     1321 aggctatgct ggctaacagt aaggtcggcg tcgtcctggc tatgcggttg aattgccgat
     1381 acgaagggct gcactttatt cccctgtccc ctaagctgga agggtcgacg gctctggcct
     1441 ggaaaaaaga acaagtcttt tcaccggcag tcgctgcttt tcttgaattt gccaagaaat
     1501 acgttacagg catgtctcac gatgaccgat aagcattagg aattgcagct acatattgat
     1561 acaataaagg tattccggga agggagtacc ttttttggtt atagagtcct ttccttttcg
     1621 attccggaag gggcagctgc ggttgcgtaa aaccagggac cgctcatctt tttccttgaa
     1681 ggatgatcta tcaggacagg ccttttgtat gaggatcagc attcatggac cgttgaagga
     1741 gcgatgacgt atgagaatta tcccggcaga cataaaagat gtaccggaac tatatcagct
     1801 gcagttgctg gcttttgaga gtgaagccgc tatgatcggc agtcgcgacg ttccggcact
     1861 ccaggaaaca gaggaagagg cacgccggga ttttgcaaat tgggatacct ttaagctcga
     1921 agacgacgac ggcacgatca ttggcgccat tcgctgccga caggatacgg gcttcatcga
     1981 tgtcggccgc ttgatgattc atcccgatta ccgccgctgc ggcctggccc aaaagctcct
     2041 tgccgaggtg gatgtcttgt atccggacgc ggtgaaagag ttgtacactt gtaccaagag
     2101 ctggagcaat ataaagttgt acgaaaaaat gggctacaag gcgtataaag aacatcggga
     2161 agacagcggt ttatctttcg tatacatgag aaaatagggg gcttcaaagg gctccggctg
     2221 ccgtcgaatg ggattttttg aaccgacttt caggagctgc ccgtgttgga aggagagcgt
     2281 atctatgatt cannnnnnnn nnacctttgt aaacagagcg tcttgacggc acttcagacg
     2341 gggtatcgcc tcctcgatac ggcggcctgc tacggtaacg aaagggccgt cggagacgcg
     2401 gttcgtgaga gcggcatcga tcgggaggac gtgttcgtaa cgtctaaggt ctggattcag
     2461 gatgccggct atgaaaaaac gagggcttcc tttgaaaaga cattagaaaa tctacagacc
     2521 gattatctgg acctctatct gattcacatg ccttatggcg actaccatgg gagttggcgg
     2581 gccatggaag aactctacca tgaagggaga atccgggcta ttggggtgtg caacttctta
     2641 ccggatcggc taatcgattt gatcctcagt cacgacgtcg tgccggccg
//
LOCUS       DAWDXZ010000222         2687 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7677_length_2687_cov_7.751140, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000222 DAWDXZ010000000
VERSION     DAWDXZ010000222.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7677_length_2687_cov_7.751140"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          615 a          666 c          711 g          695 t
ORIGIN      
        1 gcggacaccg ctttcatcag gatcgctgat attgatcagg gtgatgatga gatccttccc
       61 ttcttcgatt tcaacgtgaa gttcttcatt tttcttcatg ccgaagaaga aggtcggcgt
      121 atcgaggacg ctgacatcgc cgaatctctt gaccttttca ttgtagtctt caaagacttt
      181 cggatactgg cagtaagcat tgatgtcttc ggcttcgtgc ttgtagccgt tttcagccaa
      241 tttagcacgg acggcgtcga agtcgaccgg tgccaggctc ttgcccggcc gttccgtaag
      301 gggtttcttt cctttgagga taatcttctg gagtttttcc gggaagccct gatacggaat
      361 gccgatacgg ccttcgaaga attcgacgac ggactgcggg aagtcgagga cgtcgccttt
      421 atcgtagata tcctgttcag tcaggttgtt ctggaccatg aacaaggtca tgtcgccgac
      481 aaccttggac gacggcgtaa ctttgatgat atcgccgaac atcatgctga ccgcatggta
      541 catctttttg atttcattcc agtggtcgcc gaggcctaca gccttagcct gctggcggag
      601 attggagaac tgaccgcccg gcatttcatg ctggtagact tccgtattcg gataagcttc
      661 ggctttgtcg acgcctttgt aatacggacg aaccgtagcc cagtagcggg acatttcttc
      721 catagcattg acgtcgaggt ccggctgacg atccttgccg ctcaaagcgt aatacaaggt
      781 gctcatgctc ggctggctgg taccgccggc aaaggccgaa taagcaacgt cgacgatatc
      841 gacaccggca tcgatagccc tgctgtacgt cgtaatggca ttacccgaac catcatgggt
      901 atggaggtga atcggaaggt caacggcatc tttcaaagcc gataccaaac ggtaagaagc
      961 ttccggtttc aagagacctg ccatatcttt aatggcgatg atgttggcac cggcattttt
     1021 caattcctta gccatgttga catagtatgc caaatcgtat ttattccgcg acggatcgag
     1081 gatatcgccg gtgtagcaga gggctacttc tgccaattta ttgttttcac gaacggcctg
     1141 aatcgtgccg tacatattgt ctaagctgtt aaggctgtca aaaacacgga agacgtcgat
     1201 gccgttcttg gccgagaggt cgatgaagtt tttgacgaca ttgtccggat agctggtgta
     1261 gccgacggca ttagcaccac ggacgagcat ctggagaagg atgttaggag ccttttcacg
     1321 catctggcgc aggcgaaccc aaggatcttc ataaaggaag cgataagcta cgtcgaaggt
     1381 cgccccgccc cagcattcat acgagaagag gttcgggagt ttcttcgagg ctgcttccaa
     1441 gacacggagc atatcgatgg tgcggacacg agtagcaaac aaggactggt gcgcatcacg
     1501 catggtcgta tcggtgaaga ataccttatg ctgttctttg atccactggg agaatttttc
     1561 cggtcccatg gcatcaaatt tctgtttcgt gccgtccgga tacgggccgt tttcaacact
     1621 cggcagttcc aaaggttcaa attcagtctt ttcctggtgg ccggcaccgg catagccgtt
     1681 aatcgtcgta tcgctgatgt actctaagag tttcgtgccg cggtcctgta cgaccggcag
     1741 ttcaaacagt tccggatgat tgtcgatgaa gttgacatta tacgaaccgt cgacgaattc
     1801 aggactcttc aggacgttga tcaagaagcc gatattggtc ttgacgccgc ggatacggaa
     1861 ttcctgaagg acgcggagca tcttctggac ggctttggca tgatcgaggc cgtaagtcgt
     1921 cgacttgacc aagagcgaat catagtacgg cgtaataatg gctcctgtat aagcattgcc
     1981 gctgtcgaga cggacaccaa agccgccgcc actgcggtat accgtgattt taccggaatc
     2041 cggcatgaag ttattcagcg gatcttcggt cgtaatacgg cactggatgg cattgccgag
     2101 acagtggaca gcatcctgag acttaatgcc gatttcatcg ctgtcgaggg cataaccttc
     2161 agcgacttta atctgggtct ggacgatgtc gatacccgta atcatttcgg tgaccgtgtg
     2221 ttctacctgg acacgggggt taacttcgat gaagtagaag ttgccgtcag gcgtaaccaa
     2281 gaattcgacg gtccctgcat tgacgtaatg aacgtttttc atcaacttca aggcagcatc
     2341 acagattttc tgacgaagtt cgacgggcaa ggccgaagcc ggagccgttt caacgacctt
     2401 ctgatgacgg cgctggatag agcagtcacg ttcaaagagg tgaatgacgt tgccgtgttc
     2461 gtcgcccatg atctggactt cgatgtgctt cggattgatg atacattttt cgagataaat
     2521 ttcatcgctg ccgaaagcca gctgcgcttc cgattttgcc cgttcataag catcgcgcag
     2581 ttcttccatg gagtcgacca tacgcatgcc gcggccgccg ccgccgttta cagctttcag
     2641 cataatcgga agtccgactt ccttggcaaa attttcgact tcttcat
//
LOCUS       DAWDXZ010000223         2681 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7701_length_2681_cov_9.515613, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000223 DAWDXZ010000000
VERSION     DAWDXZ010000223.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7701_length_2681_cov_9.515613"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1475..1484
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          765 a          675 c          618 g          613 t
ORIGIN      
        1 ataaacagca ttgacagagg actcggcctt agcccgagtc gccgaagccg tcgcatctac
       61 cgacaccggc gatgtcatgg ttccatcggc agcaaaaacc gtcccgccgg tcatcaacaa
      121 gcagaaaact gctgccatta ccttggtatg cttcatatgc aagaccacct ttctagaatc
      181 attttgagtt tcactctcgc tattcctcag tatagcatag aaatgtctct ttatggtggc
      241 aatttgttct atattattca caaaaatcgt cggcgcacaa aaaaaaactg tgcatcgggt
      301 aaatcccaaa tgcacagtct tctttcatcg tctcaacaac acacgcagca aatacgtaaa
      361 tatatgccag ccatagagga gtctgtcggc cagttgtgaa catcgtttac tccggtaaac
      421 ggcataaccg gagccggccc ctaagacgaa tcctccaagg acatcgctga cgtaatgaag
      481 gcggcagagg acacgagaaa cgcctaaaat gacggacaag gccaaggccg gaagccccca
      541 aaggtttcgt cgtaaaagca gcatgacggc gacggccata ctgttcatag catggttgct
      601 gggaaaagag gcattagcct tatgtttaac taaagcagcc cctttaccgc gagcaaaagg
      661 cctcggccga taccacaatt tgccaatgaa ccacgaaaca gatgaggcga gaatgaccga
      721 aaacaagcaa taaagcagcg ttttccgttc tttccgttgt tccttctcgt ttttagggcg
      781 aaaccaggcc cccaaaccat agaggataaa caggccgtga ccaaagcgag taaaccacga
      841 taaaggacga agccacagat tatagcgagc cagaagaaat aggatgagtc gaaccatgtt
      901 gccctccctt taccaaatca acattgcttg tcaatagcct ttgccatcgt cccggccata
      961 tcagccagcg aaatgcggcg catttgctct tccatcgctt gctgtacctg actcaactta
     1021 tcgtcgaggc tgaggtggat ggttttaccg acagggcatt ggggactggg gtgctcatga
     1081 aaatgaaaca gagaatcgct ggggatgcag tcaacggcat ggtaaacgtc gtataaactg
     1141 atgttctcca agggcttttt caacgtagcc cctccggctc ctcgcttgac gtcaataaat
     1201 tcggccgcct ttaactgggt aagcaggttc cgaataatga ccggattggt accgacactg
     1261 gaagcaatga aactgctggt cactttttcg gtcttttcaa aatatgcaat gcacagcaag
     1321 gtgtggacag ccatcgtaag gcgactggaa atctgcatcc ttaaagagcc cccacttcac
     1381 ggagaatggc caaagaaggc gtgcaggccg ggcagtcaca gaacttagcc ccactggctt
     1441 taagatcttt ggcatgatcc aagagctgag cagcnnnnnn nnnngagcag cagcatctgc
     1501 ccccaaaacc ttcgcagccg tatccgatcc ggcaaagccg aggagttcat caatcgtcga
     1561 tacgccttgc ttcaaggctt ccacataggc cggagccgct gcctctttat cagcagcatc
     1621 aagccattcc tgggccgctt ttttcaaggc agggctgcag ctgggtgctg ccactaattc
     1681 ctgagttttc gtaaccataa agtcgatgag tttcttatcc ataatagtac ctcctaagaa
     1741 aaatagcaca tctttagtta taaccaataa acttacaact atatcctagt cgatttaaaa
     1801 agtaatgtca atggttacaa tataatcgac ttaaccccct cccattacaa ataaatcaaa
     1861 ttaaatttat ttactattat ttaaattcat ataatcagct tcattaagcc atttattatg
     1921 caaataaagc tggaatttaa actaattcga actagtgtaa acgtctataa aaaatctgtg
     1981 ttgccacgag attgtcccag tctggcgcta aaacacggaa aacaacgaat taccagaagc
     2041 aagaattcgg gtctggaaag aggggccgat catttcggag ctcacaaaaa tcgaaatgct
     2101 gcggaaaagc ccgaaaagga cgtggatgac cgtgcgacta acacacggga catcgaggaa
     2161 ggttttaacg gtgtcagcgc cgcactaaaa agcggttgca acgtctttca aggttgtcgc
     2221 ccccggcatg acggcaacgt ggcagcggtt gcaacgttta atcataacta tatcgaggca
     2281 ggcgatgcaa agacctaatc agaggctgca agatcgctcc atcgctcttt aggcgataaa
     2341 gaagaagctt ccgcaaaaag tatcattgcc tcaaggagaa aaaaagcctc ggagacgaat
     2401 ctccgaggct tttcgtatgt ttaactggaa tacagattta agcgcgtacg gctttgacgg
     2461 cagcgatgaa cttcttggcc gtttccgtga tttcagcagc cgttttgccg ccacccgtca
     2521 gggctccgcc ggcaccgaca gcgacgcagc cggccttaat ccattcaccg gcgttgtcga
     2581 cgttaactcc gccggtaggc atgagtggtg cctgcggtaa ggggccgtgg atggctttga
     2641 tagccttcat gccaaccact tcacccggga agattttgac g
//
LOCUS       DAWDXZ010000224         2680 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7704_length_2680_cov_6.648762, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000224 DAWDXZ010000000
VERSION     DAWDXZ010000224.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7704_length_2680_cov_6.648762"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          674 a          724 c          619 g          663 t
ORIGIN      
        1 gtcccgcata tgccgcaggc cctgttcacc gtatgtatcg tacacggcct gcaggcggtc
       61 gatcatcgcc tgcttttggg ccggtatctg tccgtaaatc gccgttatga tggaggcatt
      121 ggtggcgtcg atgagggtct ctatggttaa atccttcaag aattccttca attccaagat
      181 gcgttcccct tccgttgctt cctgaaattc gccgcttcgg acgtccttca tgagcggtgt
      241 atccgggaaa atcgtaaggc cggcaatgag gatgcggtcg ggatggagct ggttaaaaac
      301 ggcagccgtc tttttggcat gatccaagcc atagcccttg ccgccgaggc cgctcaggaa
      361 gatgacttcg taaggcatgc cggcctgatc catcttagac aattgaggca cgatttcatc
      421 ggcatgatag cccttattca tgtaggtcaa gaccttttca tctcctgatt cgacaccgaa
      481 gacgatgccg aaataaccga ggtccttcaa ttcattcaac tgatcagaag tcttattgcg
      541 caggctgttg acccggccgt agccgccgat ggtcttaacg aaaggcaggt actgatggat
      601 gagtttggca atgcgcttca atttctcgaa agtcaggaga aagggatcgg ccccctgcag
      661 ccaaatacgc gggaagggca caccgtaact tgccatttcc ttcaagtctt cgacgatttc
      721 cgattccggc gaaacggcaa aaggcgcctc cttataataa gtgcagaaac gacaggcgtt
      781 gtgactgcag ccgctggtta cctgtaaata ggcactgttg gcttcataag gcggacgtac
      841 gacattgctt gcaaaatgca tggacaatct cctctctttc tcccttaaac ggctctcatg
      901 ctgtgacgcc agcgcgacaa ttcagcttcc acctgttcgt caaaccgatt gattgtcttg
      961 tcgagggccg acagaatctg ttccctttcc tgaggcaagg tcacttcaaa acgaacgctg
     1021 ctggtagacg taccagaatt gatggtaatg ggaatcttca attcggccaa gagggtacgg
     1081 atttcttcga ttcgttccct ttccgtcgct tccgtccatt cgccggcttc catctgatca
     1141 tacagcttcg ttcccggcag gacagacacg ttggtcagca tcatgtagta cggatgaagc
     1201 tggttgaaaa tcgcagccga cttcttggcg ctttcaacca gctttccctt accggccaga
     1261 ccgccaaggt aaattatgcg gtaagtaatg ccggcctcat ccattttctt gccggcgtcc
     1321 aaaatatcct gagccgtata gcctttattg acgaaggcca agacgtcatc gtcccccgtt
     1381 tcaatgccga tcatgatttc atcgaggccg tgggccttaa tctttttgat gtcttccacc
     1441 gatttctgtt tgaagtcaga aatcgtggca tacgtcgaaa tgcgggcatc gggataatat
     1501 ttcttcatca ggtcccagac ggcaacctgt ttttccgtac tgagaacgaa ggggtctccg
     1561 ccggaagccc aaatgttctt agcatcgggc cattctcgct tagcgtcctt caaatcttcc
     1621 tcaatctgag aaaggggtgc caccctaaaa ggcgtatctt tatagaaatt gcagaacgtg
     1681 caggcattgt gcgagcaccc tgccgttact tcaataaata aactatccgc atcggtctgc
     1741 ggtctgataa tcggtccgtg aaaatgcatg gtaagattcc tttcttcaaa tctaaaattc
     1801 gctcattata gatatattaa tcctaagaaa agggggcaag atttccaatg accttacccg
     1861 cacttatatt atatctattt ttatctatat gtcaatatat taattcgttt tttttttttg
     1921 ttttcttcct gctctcccct ttttgaatca atcgtcgtat aatagattca agtaatccac
     1981 atttattttt gaaagcaggt gatatacatg agtaccgata cgaaaaatga aaaagccatt
     2041 acggccgccg gcaatccggc caaaccgaca ggcgaagcgg gtgaacagat gctggcccgc
     2101 atgaatgaaa gccacggccc cctcacggcc tggggcctct cccactttac ttggcgcggc
     2161 gatgaaaccg tcctcgacat cggctgcggc ggcggggcca acttaaagcg catgagcagc
     2221 cacgtaacca caggccatct tacaggcgtc gattactccg agacctccgt ccagacatca
     2281 cgccagacaa acgaagaagc tataaaagcc ggcaagatgg acgtctccca aggctccgtc
     2341 gaaaaactgc cctttaccga cgacagcttc gataagatca cgaccgtcga aagcttctac
     2401 ttctggcccg atccgccaga aaacttgaaa gaagtcagcc gcatcctgaa aaaaggcggc
     2461 accttcctgc tcatcgccga aatctacgaa cggcctgacc tcagcccggc cacccgagaa
     2521 aacatcaaac actacaacct ctacaacccg acgccggaaa ccttcgaaga tatcttccgc
     2581 aacgccggct tcacggccgt caagctccac atcgagccta aagaaggctg gatttgcgtc
     2641 gaaggaacaa aataatatcc gcacatcaaa aaagagagta
//
LOCUS       DAWDXZ010000225         2676 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7717_length_2676_cov_11.206410, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000225 DAWDXZ010000000
VERSION     DAWDXZ010000225.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7717_length_2676_cov_11.206410"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          522 a          695 c          792 g          667 t
ORIGIN      
        1 gcgggcaggg atagggcgac ggtttggcct ccccgtcctt cccctcttcg gtaataggtt
       61 ccgtatggcg gcacttggga tagttgctgc agccgtaaaa gtcgccgtac ttgccgtggc
      121 gcttgaccat ggggctgccg cattccgggc aggccttggc gccttcggct ttcatgacga
      181 cgccgccggc agccgtgacc aaggctttta agaaatcgag ttggtcgcgg aggaaggctt
      241 cgaggttgtc cttcccgagg ctcatctgat agagccgttc ttcccaaatg gccgtttcat
      301 cgggatagag catttcgtcg ggcaaagcgt cgacgaggag ataggcattg tccgtcggct
      361 tgaggtattt ctttttccct tcggccatga ggaacttccg ctggatcagc tcttgaatga
      421 tcgtcgcccg cgtcgcttcg gtaccgatgc cctggacttc tttcaatttc ttcttcaggc
      481 tgtcatcttt gacgtattta taaatctctt tcatagccgc caataaggtc gacggcgtaa
      541 accgcggcgg cggcttggtg gccttcttgt caacctggcc gtttcggtaa tggacggcat
      601 cgcctttgcg gacgacggga agttctccct tttcttcgtc gtcttcattc ttgtcgctct
      661 gataaagggc tttccagcca agaacggtaa cggccttgcc gtgggcgacg aaggtctcgt
      721 cggcgcagtc gatggtaatc cgcgtctggt cgtattcgtg gatgggatag aactgggcca
      781 gatatgcctg ggcaatgagg taatatatgc gctgctggac ggcagtcaat ttgtcgaaag
      841 ggcaaggaac ggtcgtcggg atgatggcgt gatgggccgt aatcttgctg tcgttccagg
      901 cccgactctt gatcttgccg tcagccccgg ccgcccattc aggaagcttc ccggccggga
      961 tggccgcgat attcgctaag atgcggggca cgtcttttcg ctgattgagg ggcaggtatt
     1021 cacagtccga gcggggatag gtcgtaagct tccgttcgta cagggtctgg gccgtgtcga
     1081 ggacctgctg gggcgtgtag ccgtagcgct tgcctgcgag gacctggagg gacgacaagg
     1141 ataagggcaa acgctggggt tctttcttct tggatttctt attctcgacg atggttccgt
     1201 cgggattggc cactaagcgg gataagatat ccttcgccgc ttcttctttg atacagcgcc
     1261 cttcggaatc gaggccggcc tgggtatcct tcggctgcca ctgggcgcgg atcatgccat
     1321 ttttatgctc aaaatcggct ttcacgacgt aatacgtaac cggcacgaaa tcgtcgagtt
     1381 cccgctggcg gcggacgacg agggctaagg tcggcgtctt gactcttccg atgggaaaga
     1441 cgacgcgatg gccctgacga cgagccgaca aggtataggc tcgggacagg ttcatgccga
     1501 tgagccagtc ggcccggctc cgagctaagg ccgaatcctt caagtggacg aagtcgccgt
     1561 tgctgcgaag atcgcctaat gattcatgga tgctcttttc atcaagggca ttgaggagaa
     1621 tgcgctcgac aggcttttcg tttcccacgt agtcgaggat ctcatcgatg agcagctgcc
     1681 cttcccggtc agggtcaccg gcgttgacga tggcatcgac atcgggactt gaaatgaggc
     1741 cttcaatgat atggaactgc tccttgctgg ccggactgat gaggagtttc cattccgtcg
     1801 gaatgatggg taaatcttcc gccttccagc gtttatattt ggggtcgtag tcttgaggtt
     1861 cggcctgctg caggacgtgg ccgtaagccc aggtgacgat gccgcctccc gtacggatac
     1921 agccgtttcc ccgccgatga ggttccggca ggcacttagc cagttcccgg gccatactcg
     1981 gtttttcggc aataaaaagt ttcataccac atctctcttt ctgcaataga ctgtacctta
     2041 tagcatacca tattttgagg tttctttaca gagtgccatc ggcacaaaaa gcaacccagg
     2101 cctatacggc ttgggttact ttggctaatt gtccgacggc ccggatgacg ttggcaaagg
     2161 ccggcggaat atcgtctttt tcaagggatt ggccaaggag gcgtccgaga atgacgcctt
     2221 tattactgta cagccaggca aaatagtaga cgtacagggc ccgcttattg cgagggttga
     2281 cgttccacaa ccggcgtgcc ggatagtcat ggtaattgat atccagctta cggcaggcct
     2341 ttttgatcat gtccgtcgta gccctggcgt ggtcgccgca ggccgtgttg atgatgcggt
     2401 ccaaatcgcg gcgccgtccc ctgtcgagga gggtcttggc cagatccatt tcaaaacaga
     2461 tttcatgggt aaaatagaca ccgcgttcac ccttgtgggc cgccttgacg tcagcgtcat
     2521 acaaggcggc gacgggaatc ttgaagtatt ctaaaagagc tttgatctta gaaatagagc
     2581 tttcaccgcg ggcgttgata agacagatgc cgtagtagtc aaagggtatg tttaaggtca
     2641 cgccgaactg ctggaagcag ccgtactccg tttcgc
//
LOCUS       DAWDXZ010000226         2668 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7735_length_2668_cov_7.950249, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000226 DAWDXZ010000000
VERSION     DAWDXZ010000226.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7735_length_2668_cov_7.950249"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          603 a          747 c          627 g          691 t
ORIGIN      
        1 actgtagagg tctgtacgtg gggctgtttc tggacaggag ttcgactctc ctcatctcca
       61 ccaaattcaa aggactatcg tcactcggta gtcctttttt attgaaaaaa atgcctatac
      121 ccttattttt ttcgttttca tccttgacaa accttttacg caggtctatg ataaggtgca
      181 taacttaaac acgatatatt gacattcgat gacgagaaag agtacagctt tgcaaaagca
      241 cacagcgagc cgggtatggt gaaagccggc agccggagga agcatggaaa atcatctctg
      301 aggattgcag ttgaaatgag taaactgcac cgtctgatcc acgttacagg aaatgcgtat
      361 gatagtacgt tacaaagcgc cttgcgtctt ttggcaagga atatcgggtg gtaccgcgga
      421 taaaacttcc gtccctttag tggggcggga gtttttttat ttcatgttta tcttatgaca
      481 atctaactta tcattaggag gaatgcatca tggaaaacat caatttcgac agtcttaaac
      541 ggcggagaga tctgcgcatt attttaccgg tctttctcgt ttcgattatt gcctgcatcg
      601 accgcgtcaa catttcctat gccaagctga ccatgaccca gacattgagc tggctgtccc
      661 ctgaagtata cggttttggc gccggcattt tcttcgccgg ttacctgctg ctggaaatcc
      721 ccggttccct ctttgccgct aaattctcgg cgtcgaaatg gattgcccgc atcatgttta
      781 cttggggtgc cgtcagcctt tgtatggcct tcgttacgac gcagtggcaa ttctacctct
      841 gccgcttcct cttaggtgcc tcggaagcca gtctgtatcc agtcatttat tcgctcttat
      901 tcccccgctg gttcacagcc gctgaacggt cccgggcaac gtctctcatg ctgacctccc
      961 tgcttctttc gaccatcgtc ggcgccccca tcgccggcgt ccttttggaa cactctttct
     1021 tcggtctctt aggctggcag gaactgttca tccttgaatc gattccggcc ctcatctttg
     1081 ccttctattt cttactggcc gtcaaggacc gtccggacca agtccattgg ctgacagacg
     1141 ctgaaaaaga atacctgacg accatgtatg aacaagaaca gagagccatg cagcacgtga
     1201 aaaagtatac cgtcctccaa gcctttaccg atgctaaagt cttaaaactc tgcttcatct
     1261 acttcctttg ggtcgtcggt ttctggggct tctacttctg gatgccaaca gtcctgaaaa
     1321 acctctctgg ctggtcgacg tccctcatcg gcggtgccat cgccattccc atggcagcgg
     1381 ccctcgtcgt ccagttgatc atcggccata cggcaaccgt taccggcgat aaggtatggc
     1441 acgtcttcgc agctctcacc gtcggcgccg tcggtctcgg cctgtcgccc tttgcgcaca
     1501 acatgggcgt cgccctgttt ctcatctgcc tttccgctat cggcatccac gcttccatgg
     1561 gcgtctggtg gaccattccg acgaccttcc tctccggtcc ggccgctgcc ggctccatcg
     1621 ccctcatcaa ttcctgcggc aacctgggcg gcatgttcgg ccccaatatg atggcttggg
     1681 tccaggctca aaccggaagc ttcgccatgg gctactactt aatggccatc ttcatgttca
     1741 gcgccgggct cttagtcctg accttgaaat ataaggtaaa cggcacggta aaagaagatg
     1801 tacacctcga tacacgaaca aatacgacag gttacaccgg cacccagcct agcgtaaccc
     1861 attaagatac aaaaaaatcc cacggcctct cataacactc gaaaggacgt gggatttttt
     1921 tgacagaatt aatgaaatag taaaggaacc gtagcttcta agcccttgcc agaaatactc
     1981 tcaacgcttc ttgggcaaat acggcgtatc cttcggcatt gggatgaact ccgtcagcat
     2041 agagattttt cccggatgtt tcttcgccat cctgcaaagc ctgataaaag tcgatagccg
     2101 cgcaccccgt attggcacta taatccaaaa gccactggcg gtagcggcgg agtatctcat
     2161 tgtgccgatt cacatcgccg gcatcctgcc agccatagta ggcactttcc ggcttcgtca
     2221 aaggaggaat gccgatatac accggttcct cctgggattt taacagcgaa accatacttt
     2281 ctatattgcg gcagacggag gaaaagtcct tatcgaggag gatatcgttg gaccctccca
     2341 tgatgaaaaa ggcatcgtag ccgacgaggt cgatctggtg caggcgaaag accatgcccg
     2401 ccgtcgtgtc gccgcactgt ccccggttat cgatctgaat cttgccgtct gcagccacca
     2461 aggtcgtcca ccgctctccc ggaagcgcat cgtacccata ggtcaaacta tcgcctaaac
     2521 agaggatttt catgaccgga tccccttttg aacggctgct acggccgttt ctgccgaaag
     2581 ccacggcgca tcgaagcggc agccggcttt ttcaaggtcc atctggcagg tgaacatctt
     2641 ggggatagca ctggcataaa tattgtgg
//
LOCUS       DAWDXZ010000227         2649 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7790_length_2649_cov_11.444873, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000227 DAWDXZ010000000
VERSION     DAWDXZ010000227.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7790_length_2649_cov_11.444873"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          616 a          666 c          654 g          713 t
ORIGIN      
        1 ttgcaattta gacaatcttt tatgaaatac ttaacaaatt tgtaccaatt tacccggatt
       61 catgatatta ttgggatcta aagccttctt gatggccttg atgacattta gttctgcttc
      121 cggagtacag agcgcaaact cttcgagctt cttaaaaccg acgccgtgtt caccggacat
      181 cttgcccccg agggcataga cccgcgggaa gaggtcgcgg tggaaggcct tgatcttttc
      241 aaaccattct tccggcgaca agcctttggc attcaaagga agaacgtgga tattcccatc
      301 gccgatatgg gcaacggtga cgcagtagag gtcgtacttt tcgcgaagtt ccggaatctg
      361 cttagtgata tcaggaatct tatcgagggg aacgacgaag tcttccgtct ggaacatctt
      421 gtcgacgtcg cgggccgctt cggcaaactg cttgcgcagc ttccaaatgc gcttatcggc
      481 ttcaaattca tcgatagaac cattttcttc agcaagatca cagagctgtt ccatcttacg
      541 gtccgaatcg tcttcgctga acgattcgac agtaacgatg acgtagtcgc agccttcatc
      601 gacatgaggc attttcatat ccaagaactt gcaggtcatg cgaagggctt cattgtccat
      661 gaattcgata ctggtcggat cgatgccggc tttcaagatt ttattgggaa gggcaaaagc
      721 ttcttcatcc gtcttgaata cgcagaccat attgaaggta taaggcggca tggggcgaag
      781 cttgaccgtg acttccgtaa tgatgccgag aacgccttca gaaccggcga agagctgttc
      841 caggcaaaga ccggtagagc atttctttaa gcgggcaccg gcatcgagga tgtcgcccgt
      901 cggcgtaacg actttcaggc tgtaaatctg attgcgcgtc gttccgtact tgacagcctt
      961 attgccgccg gcattattag ctacgttgcc gccgctcgac ggatcgccgg cgtaaaggag
     1021 gccgtgttta gccgcttcgc gctgcaaatc gccggtaatg accccggtct ggcagacggc
     1081 atacatgttg tcggcatcga aggtcagaat ctgattcatc cgttccaatt cgacgacgat
     1141 gccatggtag acggggatag cgccgcaggc aacgccggaa ccggccgaac gcggcgtaat
     1201 gggaacgagg tatttgttgg ccagtttaac gacttcagcc acttcttccg tcgaacccgg
     1261 gaatacgacg acttccggtt tgtggaaata atgagagtta ccttcttcat cggtctgata
     1321 ccgttccaag taatcttcgt cagtcttgac gaatttatcc cccaggacat ttcgtaattc
     1381 ggctaataat tccggtgtaa caggattaaa tgatgctgcc atatataaac gcttcccctc
     1441 tgtcagtctg gatttaagta ttccaaaaag ctatagtaaa ttcacttctt catggaagtt
     1501 gaacatagta taccatatcc caaagacttg taaagccact tttttcttca tttcttagga
     1561 ttatcccggt ttatcttcta atcttgaaac gaaagaacgt gatttttaca taaagtttta
     1621 cgttttacgg cagcatcgtt tttaccgatt atatttattt tttatatctt ctatgcttga
     1681 ttaagattca gctagcgttt cccatttttc gtaggcgtcg tcaagctcag cctgagccgc
     1741 ttcatacgct tccgtcaggc tcatcatggc ctcggcatca gtctgatttt caggcatgtt
     1801 catcttgact tcatacatct taagcgtcgc ttcgagttcg gcaatcttca gctccaactt
     1861 ttgaagcttc gccccttggc cgtacacggg cgctttttgt acctttgcgg tctgaaccgg
     1921 ggcagacggc ttcttggccg cagtatcttt ctccggcagg gactgcggat tgttttccgc
     1981 agccaaggcc tcggcttccc ggactttttc ccgataataa ctgtagttac cgacgtagtc
     2041 cgtcaactgt ttatgttcga ggaccaaggt ccgccgggtg attttatcga ggaagtagcg
     2101 gtcatgagaa atgacaagat aggtcccgcc aaaatcaagg agggcctgtt cgaggacttc
     2161 tcgcgtcgga atgtccaaat ggttggtcgg ttcgtcaaga atgaggaaat taggcccctg
     2221 caaaaagagt ttaagcaggg ctactcgagc ctgctccccg ccgctgaggc tgtcgatgac
     2281 tttaaagacg tcatcgccgc ggaagaggaa gtgacctaaa gccgaccggg cttcatcttc
     2341 gccgtaactg aaggcattca tgatttcatc aagcaccgtc caagccccgt gcagttccgt
     2401 atgctcctgg gcaaagtaac cgatatggac cttgctgccg agcgtgatat ggccgtccgt
     2461 aggttctttt tcgcccataa tcatgcgaat gagggtcgtc tttccggccc cgttaggacc
     2521 gatgagggct gccgcttcac cgcggcgcag gaggagcgac aaatggtcaa agagctgcct
     2581 gtcggcaaaa ccgccgctga ccttatcgag ggtcagtacc ttttggccgc attcttcagc
     2641 aggcttaaa
//
LOCUS       DAWDXZ010000228         2649 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7791_length_2649_cov_7.600231, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000228 DAWDXZ010000000
VERSION     DAWDXZ010000228.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7791_length_2649_cov_7.600231"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          524 a          812 c          678 g          635 t
ORIGIN      
        1 cttcgacgac gacgccatgt ttgataaaat catggccgat gtcgatggca gtcaataagg
       61 agggatgcag atgaatccat tttcaaaccc ggcgggcggg cgaaaccgaa ttattcccgg
      121 cttcggcctg accctgggcg tgaccctggc catgctgtcc ttattgatcc taatcccgtt
      181 gatatcgatt ttaggccacg cctttcagct gacggccaat gactacgcca agctcatcac
      241 caacagtgcc attcgcaatg cttttatcac gacccttgga agttctttct tggcggccct
      301 gatcaactcc atcttcggtt tcatcctggc ctgggtcctc gtccgctatg aatttcgcgg
      361 caagaccctc ttaaacggtg tcatcgaact gccttttgcc ttgccgacgg ccgttgccgg
      421 cattactctt tccaagctct attccgatac gggcttcttc ggcagcttct ttgccggcta
      481 cggcattccc atcgcctata cccatatcgg ccttatcatc gccttggtct tcgtcggcat
      541 tcccttcgtc gtccgctcca ttcagccggt cctggaaaat ttggatccga cctatgaaga
      601 ggcggggagc atgctcgtcg cgtcgaagtt taccatcttc cggcgcatca tctttccgga
      661 actgcggccg gctctcttga ccggtttcgg cctggctttt gcccggggcc ttggcgaata
      721 cggcagtgtc atttacattt caggcaacag tgccaagaac cacactcagg tcgtctcgta
      781 cgtcatcatg cagaagctta attacgtcga ctatgccagc gctacggcca tcgccttggt
      841 catgctcatc atcgcctttg ccatgctgtt ttccatcaat atgattcagc tctggcaggc
      901 tcggcgcgtt caatagagga ggggaatcat gaagcacagc aagacaaaaa tactcttact
      961 gagcatcagc gtcgtcttcg tcgtcgtcat gctcatcgtc cccctcattt cggtcctaat
     1021 gaattcactg gccgagggct gggactttta cgtcaaagcg ttgacgacgc cttacgtttt
     1081 gtcagccctg cgcctgacct tgatagccac ggtcattgcc gtcatcgtca ataccgtctt
     1141 cggcctctgt gcggcctggg ccatttcaaa attcgacttt cggggcaaac acctgttgac
     1201 gaccctcatc gatattccct tttccgtctc gccggtcatc gccggcctgg ccttcatcat
     1261 gaccttcggc cgcatgggct ggatcggtca gtggatcgaa gtcctgaacg attatttagg
     1321 gacggatatt cagattgtct tcgccgttcc cggcgtcatc ttggcgacga tttttgtcac
     1381 ctttcccttc gtcttccggg aaatcattcc ggtcctccat acccagggcc gcgatgaaga
     1441 agaagccgcc gccctcatgg gggccaaggg gaagacgata ttcttccgca ttaccctgcc
     1501 gcagatcaaa tgggccttat tgtacggcat cgtcctctgt acggcccggg ccctcggcga
     1561 attcggtgct gtcagcgcca tgtcgaaact gcggggcgaa acctttacct tgccccttga
     1621 aatcgacgcc ctctaccaat cggggtcgac cgatgccatt acggcggcct ttgccgtgtc
     1681 gtccctcttg gtactcttag ccgtcggcgt tctcttgctg agggtattct ttgaagccaa
     1741 gctccacaag ccatcttaag gaggtgatgt catgtacgtc gagatgaagc atatcgaaaa
     1801 aaactacggc tctttccacg cctcgcggga cgtgtccttt tgcgtcgaca agggcaaact
     1861 ggtcgccctc ctgggaccca gcggcagcgg caagacgacg atcctacgca tgctggcggg
     1921 ccttgaaaat cccaacagcg gcgacatcgt catcgacggc aaacgggtca atgacattcc
     1981 cgctgccaag cggggcatcg gcttcgtgtt ccaaaactac gccttgttcc gctatatgac
     2041 cgtctttgac aacgtcgcct tcggactcac catacagggg atggataaga agcaaattcg
     2101 tgagcgcgtc ctggagatga tcgacctcgt cggcttggcc ggcatggaag ggcgctatcc
     2161 caatcagctt tccggcggcc agcgacaacg cgtggccttt gcccgggccc tggcgcctca
     2221 tccccaggtc ctgctcctcg atgaaccctt tgcggccatc gacgccaagg tgcggcaaga
     2281 gctgcgcaac tggctgaaag agaccattca ccgcgtcggt attaccagca tcttcgtcac
     2341 ccacgaccaa gatgaagccg tggaagtggc cgacgaaatc atcatcacca atcacggccg
     2401 catcgaacaa gtcgggacgc cctttgtggc cgaattcatt gggacgcccg tcgtcatcga
     2461 caactgcagc gtcctcacgg gcttcgtcga cgtcggcggt ccgaaaagag ccattatccg
     2521 accggaattc ctcaagatct ataaacaggg gacgatgaag cgctacgcca gtgctgctga
     2581 agacggtgtc gtcgaagccg tcgtcttccg cggcagccgc ctcgacgtga ccctgcgcgt
     2641 caaggacta
//
LOCUS       DAWDXZ010000229         2647 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7799_length_2647_cov_8.113426, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000229 DAWDXZ010000000
VERSION     DAWDXZ010000229.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7799_length_2647_cov_8.113426"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2166..2175
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          610 a          804 c          701 g          522 t
ORIGIN      
        1 cgcgggagac tgccgtctcc atgctacggg aacctatgtc tgcaccgaag ggccgcggtt
       61 tgaaacgccg gcggaaatcc ggatgttcca aaaactcggc ggcgatatcg tcggcatgac
      121 caacgttccc gaagtctgcc tggcccggga agcggaaatc tgctacgcca cggtgtccat
      181 ggtcaccaac tttgctgccg gcatttccga taagcccctg acccatgaag aagtcgtcga
      241 tgccatggcc aagagctccc tgcgcctgcg ccagctcatt ttaaagagca tcgaaaacta
      301 tcccgtcgat gaagactgtg cctgccacca tacgctccag gcttacggag ggtttacggt
      361 ataatggaaa cgttgcgctg gaaaaaggga actctctatc tcctcgatca gacggttctg
      421 ccctttaccg aaacctatct gacctgtacg acctggcagg acgtccataa ggccatttcc
      481 gtcctggccg tccgcggcgc tccggccatc ggcgttgccg ccgcttacgg cgtcgtctta
      541 ggtgcccgct ctatccttga aaagcccggt gaaaagcctt ttataacggc ccttcaggat
      601 atctgcacaa atcttgacgg ggctcgcccg acggcggtca acctccactg ggccttggag
      661 caaatgatgg cctgcgcgaa ggcccatggc ggcgaaagtc cgacggcgat cgtccatgtc
      721 ttagaagaca tggccaaagc catccatgcc gacgatgtgg ccatcaatac ggccatggcc
      781 ggctggggtg ccgatgaaat cgggaaaggg gaccgcccct tgacccttct gacacactgc
      841 aatgccggag ccttggcgac ggccggcgtc ggcacggccc tcggcgtcat ttctgaactc
      901 catcgccggg gacaggtcaa gatggtctat gccgatgaaa cgcggcccct cctgcaggga
      961 gcgcggctga cggcttttga gctcatggca ggcggcgttc cggttacgct cattaccgat
     1021 aacatggccg cctggacgat gaaagaaaag ggcgtcgacg ccatcatcgt cggtgccgac
     1081 cgcattgccg ccaacggcga tacggccaat aaaatcggga cctacggcct gtccctgatg
     1141 gccaaggtcc tgggtattcc cctgtatatt gcagcgcccc tatcgacctt tgaccggtcg
     1201 attgccggcg gcagtcagat ccccattgaa gagcgcagtc acgacgaagt ccgctctttc
     1261 agaggcgctg ccacagccct ttcggatacg ccggtctaca acccggcctt tgatgtgacg
     1321 ccccatgaaa acatccgggc catcatcacc gaaaagggca tcatcagcca tcccgatacg
     1381 gaggctgtac agacattttt tgacaagcat tcattataga aagaggtatg aactgtatgg
     1441 aatcaattat tcgcgatatc aaattagcta agagcggcca tgaaaaaatc aattgggtcg
     1501 accgtcacat gccggccctc aatgccattg gcgacaaatt gaaagccgaa cagactttta
     1561 agggcaagac catctgcgtc agcgtccacc tcgaagccaa gacggcatat ctggccgaag
     1621 tccttcaccg ggccggcgct aaggtcatcg ttaccggcag caaccccttg tcgactcagg
     1681 acgacgtcgc agcagccctc gtcgaagacg gcctgacggt ctatgcctgg tacggcgcaa
     1741 ccgatgaaga atacttcgat tttctgaata aatccctcga ccataagccg gacatcatca
     1801 tcgatgacgg cggcgatcag gtccatctgc tgcacacgac gcggagcgac gccatggaaa
     1861 acctcctcgg cggcacggaa gaaacgacga ccggcgtcaa ccgcctccac ggcctcgata
     1921 aagccggcca gttgaagttc cccatgattg cagccaacga ttcgtactgc aaatatctct
     1981 tcgacaaccg ctacggtaca ggccagtcga catgggacgg tatcatgcgc acgacgaacc
     2041 tcactgtcgt cggcaagaaa gtcgtcgtcg ccggctacgg ctggtgcggt aagggctgcg
     2101 ctatgcgcgc caagggcctt ggtgctcacg tcatcgttac cgaagtcgac ccgatcaaag
     2161 ctattnnnnn nnnnntattc gacggttttg aagtcatgcc tatggccgaa gcggccaaag
     2221 tcggagatat cttcgtcacc ttgaccggcg acaaagacgt catccgcggc gaacacatga
     2281 agaacatgaa agacggcgtc gtcctggcca atgccggtca cttcgacgtc gaaatcaacc
     2341 gccccgaact ggaagccctc tcggtccgcc attttgaagc tcgccacaac atcgaaggct
     2401 atgaactccc caacggtcat gtcatcaacc tcttggcgga aggccgtctg gtaaatctgg
     2461 ctgccggcga cggtcatccg gctgaaatca tggacctgtc ctttgccgtt caggccctgg
     2521 ctgctaaata catcctcgac catcatacgg aacttgaaaa ccatgtctat gtcctccctc
     2581 atgaaatcga cgacgatatc gccaaaatca aactggcttc catggggtac gccatcgata
     2641 ccctcag
//
LOCUS       DAWDXZ010000230         2647 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7798_length_2647_cov_11.559799, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000230 DAWDXZ010000000
VERSION     DAWDXZ010000230.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7798_length_2647_cov_11.559799"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    530..539
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          628 a          724 c          576 g          709 t
ORIGIN      
        1 gcttccattt tttcatactg tgtcagctta tcctgcaggc tgtcacaccg ttctttcagt
       61 tcacaatact cccggtacac tttttcatag tcgctggcaa tattattcat gaaagcatcg
      121 acatcttcct cgctgtagcc acgaaagccg cgtttaaact ctttattatg aatatccata
      181 ggtgtaagca tggtggtatc tcctccgtac aaagggaact acatatagcg ctttaacagc
      241 acgccggttc gaccttttcg cgacgttccg gtaatttcgg ccagctccat ccgccctttg
      301 ccgcggagag aaatgacatc gccttgacga acttcctgac tgggcccttt ggcgggctgc
      361 caattgacct gtacccgttc gcccttaatc gcatcggccg ctttcgtgcg ggaaatgcca
      421 aaccccgatg aaacgatggc atccaggcga agcgaggcca ccgtcgtttt gatttctttc
      481 actttttcct gacgcggcgc aatatcgctc agggatattt cttcaatcgn nnnnnnnnna
      541 ccatcgcgat tttcgtaaag ttctgctgta aataagggac caacggagtt tccgccaaca
      601 gtaccgctcc ggcatcctgc atgatgatgt cgccgaaacg tccccggtcg atgccgaggc
      661 tcatgagcga gcccaggaca tcgcgatgcc ctaagaggcg atagcggtca tcccaactca
      721 gcttgcaggc cgtaattcca aaatcgacgg gaccgtcata gtcgacactg acaaaagcca
      781 atctgactcg ttcagcccct tcgtaaccac cggaagtgcg aagtaccaaa gacggcacat
      841 gagcctgaat ggtctctccg atttggacgg taccgggtga tacaaactca ctgacggcat
      901 aaggacgtcc cttggctgta ctgtctgcca ggtcaataag acgggcagcc aattcaccgt
      961 tatccgtccc ttgataataa cggataattc tatctctatt cttcaaaaca atccctcaat
     1021 cattaacgaa gcggtgtatt tccgccgttg ttgtcattat tcgtgccgcc ggcattccac
     1081 gtaagaggcg gggcattaaa atcgctgtag tcatcgcgtt tcccatgatc tacagtaaca
     1141 ttgctgggta cgcagaggaa gatttccttg ccgaccttct gaatattgcc gtcgatggca
     1201 taggtagcgc cgctgacgaa gtcgacgacg cgcttagcta cgtcttccgg cgtcgcttca
     1261 aagttgatga caaccggttt acggtcgcgg atgtaatcag caatgctctg agaatcatcg
     1321 aaggtttccg gttcgacgat gaccatgcgc agcggtttgg ccggagtcgg cgtatagttg
     1381 acgctggtac gagtcggtgc tacggcagga gtacggcgcg gtacgcgttc ttcttcatat
     1441 tccaattcgt cttcatattc gtccgtttca aattcgtcgt cttcattgac accaccgacg
     1501 ccgctcacga catttacgaa tctattccac atattctttg acttcttcat agtaaatact
     1561 cctccttagt aattacgtgc cccaaagatt gccgtgccga cgcggacgac attggcgcct
     1621 tcttcaattg ctatttcaaa gtcatgggtc atccccatgg acagatactg gatctggcct
     1681 tccggaaatt tcggcttcat atcttcatat aaagcgcggg caatgcggaa tatgggccgt
     1741 acgtcctcaa cgttttcata attgggcgcc atacacatca gcccccgaac acagacgtga
     1801 tccaacccct ttgccagttc acaaatcgcc agaaaatctt cgacttccat tccaaattta
     1861 ctttcttcgc gggctacatt gacctgtaaa aggatatcct gcaccttccc gatcttgccg
     1921 gcttccttat ttaccgcttt cagcaagtgc tccgaatcaa tggaatgaat cagatggaag
     1981 tgttctacgg catgtttcgc cttattggtc tgtaaatgtc cgattaaatg ccaggtcaat
     2041 ccattatccg gaaaagcctc taatttttgc atcgcttcct ggacacggtt ttcaccgaca
     2101 tcggtcacac cgcaccgggc ggcctcttcc atcatctcta cgggatgatt cttcgtcact
     2161 gcaacaagcg taattttgtc agttctgccg acacgggcag ctgcatcagc aattttcttg
     2221 tgtactgcct caagattttc cttaatcaag cctatccctc cgtcgtgcat gcaattacat
     2281 gtatttataa aacattacac ttcttttatt atatatagtt agtcctataa aagctagtca
     2341 tatttaaaaa tttattcatc tgcttttaca aaatcgcaaa ggcgcccatg cggcctgtat
     2401 gaccgccgtc acggcgatac gaaaagaacc ggtcgtcgcg gtcaaagaca cagctttccg
     2461 tcgtctcgat atgctccgga attaagccgg catcggtcaa gagaagatga ttggccttcc
     2521 ataaatcgac tcgccaggtt ccttcatgct gaactgcaca ctcgccataa gccggcccca
     2581 gatgccgaaa agtcatagcg gtatcttcac tgacttcaaa atgcgctttt gaaatagacg
     2641 gtccgag
//
LOCUS       DAWDXZ010000231         2646 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7801_length_2646_cov_9.478194, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000231 DAWDXZ010000000
VERSION     DAWDXZ010000231.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7801_length_2646_cov_9.478194"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2080..2156
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2397..2406
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          578 a          738 c          711 g          532 t
ORIGIN      
        1 gagcgcacgc ctgataagcg tgaggtcggt ggttcgagtc cacctaggcc caccacacag
       61 accatcgttt ggaattattc cggaacgatg gttttttttg aatcatggga aggagggact
      121 taatgactga atgtgaacgg cagctccagg aaatggcctt gtccattcag gaggcaaggc
      181 gggaagccgg ctgtgaaaca aacgtaacgg cgccggaacc gtcgatggat gccatcccca
      241 atctcggcgg cgaagcgagg tccggcgact gcctggtcag tatgctggcc agcggcagca
      301 agggcaatgc ggcttatatt cgctgcggaa agacgaatat cctcatcgat gccggtatca
      361 gctgccgccg tattgagaaa ggtctcaagc gttacggctg cgctctcggt gacctcgatg
      421 ctgtttttat tacgcatgaa cactcagacc acgtgggagg attggcgacg ctgttaaagc
      481 ggactgatat gccagtctac acgacggccg aaacgtggca ggctatggga cggaagaccg
      541 atggctttga ccatcgcttt gtccgcctga cgcggcgcgt ggccttgaaa gatatagagg
      601 tgacgccttt tgccatctcc catgatgcgg cccgtcccgt cggctacagc cttcgccacg
      661 gcgatgccaa aatcaccttg gcgacggatt tggggtatgt cagccccgat gtggcagcgg
      721 ctgcggctta tgccgacatc ctggtgttag aatccaatca cgatgaagag atggtccgaa
      781 acggtcctta tccctatgcc cttcagcagc ggatactcgg acgctggggc cacctgtcca
      841 acaagacggc ggcagccttt ttggcatccc ttccgacgaa aggcctcatg cgggtccttc
      901 tggctcaccg cagtgagaag aacaacaccc cgtctctggc tctccatacg atgcgcacgg
      961 tgctcgctca gtccggccgc gtcatgggtc aagatatttt attgcggctg gcctgccaag
     1021 acggcagcgt ccgctttcag gaaaggagta gtgactaagt atgcagcaca aacgtatcct
     1081 tacggccatc gttgccgtag tcctcatcgc cggcggtgtc tttggcggct ttaagcttcg
     1141 cgataccttt ttttccccga acgggaccta ccaagggacg acgaatcagc tccaggaacc
     1201 ggaaaagtct gaatccattt cgaatgcccg caatacggct gtcgttcagg cggctaagaa
     1261 ggtaagcccg gctgtcgtcg gcatcacgac caaggtctat aaccgcgaca tgttcaaccg
     1321 taaagtattg gtcggcgaag gcgtcggctc cggcgtcatc ttcgataagg ccggctatat
     1381 cgttaccaac aaccacgtcg tcggcacggc taagacggtc atcgtttcct tggcggacgg
     1441 tcgtcggccg cgatgccaga acggacttag ccgttgtaaa aatcaatatg gataatctgc
     1501 cggtagctga atttggcgac agcgattctc ttcaggtcgg cgaaccggcc attgccatcg
     1561 gcaacccctt gggccttgaa ttccagggaa cggttacggt cggcgtcatc agctccctca
     1621 accgcaccat cggtgcggaa ggccagagca tgaagctcat ccagaccgac gcggcaatca
     1681 accccggtaa ctccggcggc gccctggtcg atgcagacgg caaggtcatc ggcatcaaca
     1741 gtgccaagat ttctcaggaa ggcgtcgaag gcttaggctt tgccattccg atcaatgcag
     1801 ctcgtccgat cttacaggac ctcatcacca acggtaaagt tgtccgcccg tacctcggtc
     1861 tctacggcct tgaccagcag atggcagctc gcttcggcat gcagcttaat gctcagggca
     1921 tctacgtcta ccgcgtcgtt cccggtggtc ctctcgatca ggccggactc cagcacggtg
     1981 acgtcatcgt taagttggac ggcacggacg tgaaggactt tgcatccctc cagagcgtca
     2041 tggataagca taaagtcggt gattccgtca ctgttgactn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggca
     2161 tgaaccgtga agccaccatc gttctccagg aaagcccgca gaccgattcg gctgacgacg
     2221 atgtgagcgg caactgatga aataccatct catcaccatc ggaaaaatca aggagaagta
     2281 ccttacggcc ggaatcgatg aattcttgaa gcgcctccgt ccttacggcg acgtcaccgt
     2341 gactgtcctg cccgaagaaa agatgcctga caacccatcg ccggcccaaa aggcacnnnn
     2401 nnnnnnaaag aaggggaaaa gatgctgcgc cacgtccggg aaggatcgca cctcatggcc
     2461 ctcgacgtag gcggcaagct cgtatcgtcg gaagaactgg cagaatcctt ttcagaactg
     2521 gccctttacg gctgcagtga aatctccttc atcatcggcg gtcccttcgg cctcagcgac
     2581 atcgtccgca gaaaagccga caaatgcatc tcctttggcc gcatcacctt gacccatcag
     2641 atgata
//
LOCUS       DAWDXZ010000232         2640 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7818_length_2640_cov_7.193424, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000232 DAWDXZ010000000
VERSION     DAWDXZ010000232.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7818_length_2640_cov_7.193424"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    755..764
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          545 a          783 c          705 g          597 t
ORIGIN      
        1 cgaacttggg gggcgacctc tctcatcccc agttcggcat taccgataag tcctatgact
       61 actatctcat cggaaagcct cagggcgaaa aggacctcta cttcgatgaa gtcgtgtccg
      121 atctcagcac gcgtcttggc agcgataagg tcaaagtcat gtccaacggt gcctatcgca
      181 ttgccggcct caagggcgtc atgcccggtc tcggcgacgt caacttgggt atcatgagcg
      241 ccgatgctaa gatcatcagc gaccacggct tctacgtcat cctgcggccg acgaattaca
      301 gcaacccgac gaaggccgac gtcgaccatt tctttgcccg ggctgacaag atccaggacg
      361 tatcgggcat catgttcgtt ggaaaagaag tgttgggcta tacgccggtt gccggcgaac
      421 gaaaggatat gctcgactat acggcggaca atttgaaccg ccgcgacatc ccgttctata
      481 tgatcgagtc cgtcgatcag ctccagtaca accagcagga cggcatgtat gatctgtcag
      541 gtctcgtcca ttatcggacg gcccgggtct atgccatggc caaggaagaa ctggaaaaga
      601 ttacgccgga agaagcggct atgcgctttt acatcagcga ccttgaacga aacgtccggg
      661 tcaatctcta tcccttgtat aagaagcccc ttcacggcat gaacctgact cagaccaact
      721 tgtcctatat caagaccgta tcggataagc tgacnnnnnn nnnngctcat tccgttgaag
      781 gaccggcaga attatattct catggccctc ggtatcgtcg tcgccgtcat cggtgccgtc
      841 gtcgccaaag gagccttgtt cctccagacc tgggccatcg gctgtgctgc cgcagcgccg
      901 acagcggcca ttctcttgtc cctcgattac tggaaacaga agaagcttac gaagaaactc
      961 ggctacggcc gggtcatccg tgacggcacg ctgggccttt tctttgccgt agccgtctcc
     1021 atgatcggcg gtctctatat cgcctcgatg ctgggcaaca tccgcttctt catggaattt
     1081 gatttctacc gcggcgtcaa gctgaccttc atcctgccgc tccttctggt ggctatcggc
     1141 tacctgcgcc ggttccccct catggggcag accattgcgt cgccggcaga tcttaaggtc
     1201 tttgccaagg atttcctcaa catccccatt aagatgggga ccatcctgat cctcggcgtc
     1261 atcgccgtgg tcggcatcat ctttgtcggc cgcagcggcc atacgtcggg cgttcccgta
     1321 ccgggaatcg aagtcgctat gcgccgcttc ttagaaaatg tcatgtacgc tcgtccccgg
     1381 gaaaaggaat ttctcgtcgg ccatccgagc ttttatctca tggtagctgc cttgtaccgt
     1441 aaatggccgc agctgctgca cttcttcctg gtcatcgccg ccacgatcgg tgtcggctcc
     1501 atggtggaaa cctttgccca tatccggacg cctttcctca tgtcctttat ccgcggcatc
     1561 aacggctggg taacggggat ggtcgtcggc atcgccctga tcatcgccat tgccatcgtc
     1621 cgttacgtca cgacgtggct gggaaagcag gtggaaaacc gtgactaggg tcgttatttc
     1681 cggctattac ggctttgcca atgccggcga cgaggccatg ttggcggcta tcgtcggctc
     1741 gttaaaagac atcattcccg acgtggatat tacggtcatt accggaaact gccgaatgac
     1801 tcgggaaaat cataaggtac aggctgttca tcgcctgaat ttctttgcta tcgcagcggc
     1861 cattcgccgc tgtgatattc ttataagcgg cggcggcagc ctgctccaag acgtgaccag
     1921 cgcccgtagc ctccactact acctgtggat catgaaaatc gccttgttct ttaaaaagcc
     1981 ggtcatgctc tatgcccagg gcatcggtcc cgtccgcggc atcaaggccc ggaaggctgt
     2041 gcagaaggtc ctgcagcagg tcgccgtcat cggcgtccgc gaccgggaat cgaaggcgga
     2101 actttctgac atgggcgtaa cccgtccgcc tatccatgtg acggccgacg ccgtcttgtc
     2161 catgcatccc gtcgataagg ctatcggccg tcagattttg gaacggcacg gagttacggg
     2221 aagccgacgg aaagtcggta ttgccgttcg caactggcag ggccatcagg cctataagga
     2281 cgccattgcc gaggcagccg accgtttgca gcagcaagaa gacgtggaca tcatcttcat
     2341 tcccatgcag catccggaag acgttgacgc cggccgcgat attgccgacc gtatgaaaaa
     2401 ggcggccatc gtcctcgacg accgctattc gacggtagaa ttcatgtccc tcatgggctg
     2461 catggatgcg gtcatcgcca accggctcca cgccctcatc ttttcggctc ttatggaaac
     2521 gccggcgacg gccatttcct atgatccgaa aatcgacagc ttcatccatc tcatcggcga
     2581 tcgggtctgc gggacgacgg aatcggtgac ggcagaagcc ttagcctccc gcgtcagtca
//
LOCUS       DAWDXZ010000233         2636 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7831_length_2636_cov_6.354514, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000233 DAWDXZ010000000
VERSION     DAWDXZ010000233.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7831_length_2636_cov_6.354514"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1906..2011
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2236..2379
                     /estimated_length=144
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          526 a          633 c          615 g          612 t
ORIGIN      
        1 tcgctgcctg ctgacgctgc tgcgtcgccg tgcatatggt catatgcaca tcgagatgat
       61 tagtcttttg ttgcaggata gcggcgatac gcttgaattg ctcttgagag aacgtcgttt
      121 gtacgacgac tcccatagaa tcgcaaaagg gaatttgttg agcctcttct tccctgtcta
      181 ttataatagc acgatttgcc ccccattgtg aaatactaat tacttcggga tgggcctttt
      241 cgcctacgat tataagcttt tcaccttttt cgatgatatt tttggcgtct tgttgggcct
      301 ttttaacgtg cggacacgtg gcatctatga cttttagttc tcgatcgtcg gctttttcat
      361 agatttccgg tccgacgcca tgagaccgaa ttaagacgac agcccctttt tctacctcat
      421 ccaaagagtc taccggcgac acgccctgtt cggcgagacg cccgacaact tgcggattat
      481 ggatgatagg gcccaaggtg tgtgttcctt tttcagcctg tccggccagt tcaacggctc
      541 gtcggacacc atagcagaag ccacaggctg cggcgagagt tactttcatt tttcgtcccc
      601 tttatactga ttttgcatag tcacgatgga atcgcgaatt tcttcggaaa aggccgtgac
      661 tacgtcgtga tccgaggctt ttccggccgg agccatgatg ggttttccaa aaatgactcg
      721 aacaggcccc gtctttttcg gcagaaattc cgaattgacg atggctgccg gcaacacagg
      781 caccttggcc ttaatggcga taccggcaaa gccctcatga aaggggccga ccgagccgcc
      841 tcgggtccgc ttcccttccg ggaaaatccc caaaacggca ccatttttca ataccttaaa
      901 ggattccata accgccttgc ggtcgatgcg gccgcgatgg accggaaagg tatggacata
      961 acgcaggaac cagcccatga ccggattgcg gaagagttcc tccttggcca taaagtggat
     1021 gagatgatga cgcatagccg ttcccaaaag gggcggatca aagttgctgg catgattaca
     1081 ggctacgata aaaggccctt tttcaggtat attttcttcg ccgatgactt ccatgtccaa
     1141 gaagccgtac gttgcgacat tgaagatcct gcggatcgta ttatacataa attcatgcat
     1201 cagcctcgct ccttcataag gccgagaatg acatcggccg tctgttgtaa atcgagatca
     1261 ctgttgtcga cgacgacagc gtcatcggct tttttcaaag gggacacctt gcggtgggaa
     1321 tccatgtcat cgcgagcggc gatgctggcg gcgatgtcct catacgattc gctgccgcct
     1381 ttggccttta cttccaaata gcggcgctga gcccgggatg cgacagacgc cgtaaggaat
     1441 attttgagat ccgcatcggg aaggacgacg gtcccgatat cgcggccgtc gaggatgacg
     1501 ccgccttctt tggccatggc cttttgtaat tttaccagtt tttgccgtac gactgcaaga
     1561 gctgcgacgg aagaaacatg agaggatacg cgttgcgtcc gaatggcctc ggtgacttcc
     1621 gtatctccga cgtagacccg acacaggtcg gccattggtt caaggcggat atccacgctg
     1681 tccactaagg ccttgacggc cttgtcatcg gcgatatcga cgcctttttc cagcaccgcc
     1741 caagtaaagg ctcggtacat ggccccggta tcaatgtata aatagccgac tttattggcc
     1801 aaaagtttag ccacactgct ttttccggct cctgccgggc cgtcgatggc aatcgttaat
     1861 tttttcatag tcagccctcc ttagcagcgg ccgttccggc tacaannnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncccgtaaag gcatcgatat cgatgacttc
     2041 cccggcaaaa taaaggcccg gaacgagttt cgactccatc gtcttagggt ctatttcctt
     2101 gaccgatacg ccgccggcgg tgacgatggc ttcactgatg ggcctcgtcc cggtcaccgt
     2161 aaggggcagg gccttaaagg cttcgaccaa gtgctggcgc tgttcccggg aaatctgatt
     2221 gaccggacag tctttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ggaatgagtc gctggggcaa
     2401 cagatccttt aagccgttta cagcttcttt gcggatatat ttttcaaaat cccgacgcag
     2461 gcgggcatcg agtttttcct tcgataaggc cggttttaag tcgatgcgaa gttctaaggg
     2521 gaaagcggcg ctctttttat gggccgctgc cgtactgagc atcaggataa tcggcccgga
     2581 aacgccaaag tgggtaaaca gcatttcccc gaagtgggca tcgatttttt tcccac
//
LOCUS       DAWDXZ010000234         2630 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7843_length_2630_cov_9.446214, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000234 DAWDXZ010000000
VERSION     DAWDXZ010000234.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7843_length_2630_cov_9.446214"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1315..1324
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          585 a          710 c          711 g          614 t
ORIGIN      
        1 tcttcgatga cggcagccga cgtttcgtca caactggttt cgagtgctaa ggtgtacata
       61 gatttcatcc ttttctataa taaatggctg agccatcgat aattttcttt ggtgcagttc
      121 ataatgtaag catcttctac aggctgagaa taatagccct tgcgaatggc cgtcttttcg
      181 tagcccagct tttcatataa ggcaatggcg gccccattcg acaggcgtac ttccaagaaa
      241 acggagcggc agccgtcatg aaacagagct tccatagccg tccgcagcaa ggtttcgccg
      301 tatccgaatc ccctggccga cgggcggacg gcgatgttag ccaattgacc ttcgtcggca
      361 ataagccaca gatcggcata ggctgccacc gtcccgtcgc ctgtttccaa gacataatag
      421 cgggcgacgc ggttatcgaa ttctgcccgc agggattggt gagaccaggg ggaagaaaag
      481 gacgcctctt caatggcgat gatgtccggc aagtcctgaa gcacggccct tctgatcgtt
      541 actgacatgg tgcgctgccg tgccgttttt cccaaaggac ttcggcttca ctgcggcgaa
      601 ggtacgacgg agtcaaggtc atgcagtcgt cgtaatcacc ggcggcaaag cggacgtttg
      661 ccccgagggc tacgcttccg gcacggggaa tgaccatcgt cggcggtgcc atgcggaaaa
      721 gataggtccg gccttcgatt ttcccccgcc ctttcaagga gccgtcgccg cagaagacga
      781 cgggctgtcc ttcgccttcc aaggtatcca acacttctgt gagcggtgcg atataaatgt
      841 ctttttccgt caccagcttg gtccgatccc agcggtagac gccggcgtag acattgccct
      901 tttgggcatc gatcaaggtg catacccggt cggatgtacc gacaaaatta taggccaggc
      961 tgatgggggt ggtcacgccg acgatgggaa cattccaggc aaaggaaagt cccttggccg
     1021 tcgcaagacc gatgcgcaga cccgtaaaag aaccggggcc gacggacacg gccacgccgt
     1081 cgatttgaga tttcgtaacc gaggctttgg ccagcatgtc ggcaatatgg ggcatgagct
     1141 gttctgaatg ggtcagccgt gcctggatcg taagttcagc ccgcagggtg tcttcgtgga
     1201 gcaaggcgac actcgatacg aggctcgatg tttcaatggc taagagcata tttctgtacc
     1261 tcattttcta tagactgcca gcgatttccg gaaaagtcga agtgaaacag gcgtnnnnnn
     1321 nnnnctgcat cgtcaggcag gcggtccggg aatttttcag accattcgat gaccgtgaca
     1381 ccactgtcga tatagtcttc aaatcccaga tcgtcgagtt catattcttc ttccaagcga
     1441 taaaaatcga agtgctgaag aggaatttta ttttcatagt aatttaataa tgtaaaagtc
     1501 gggctgacga cgacatcgtc aatccccatt cccttggcaa tgccctggac aaagtgggtc
     1561 ttgccggcgc ccaggtcacc gcgcagagcc aagacagaac catccgtcag caaggggcct
     1621 aaaaaggcgc cgagggcgat ggtttctgcc tcggagtgcg tttttatatc gattgacata
     1681 ggtttatctc ctctcaaagt gatcccagcc cttgggcaag aggaccgact ctttcccgtc
     1741 atgctgcagc agtactcgcc cgacgcctgc gcggacagta ccgataggcg taacgtcggg
     1801 atagtcggat ttaagggctt taaaatcatc aggggccatg gtgaagacca attcatagtc
     1861 ttcgccgccg ttaaagacga agttcagcag gtgtttaccc gattcgtcag cccaggcctt
     1921 caattccgca ctggtcggaa cggcactgcc gtcgatgacc agttccacgc cgctggcttt
     1981 tgcgatttca ttggcttcac tggcaaggcc gtcgctgata tcattcaggc tgtggcagcc
     2041 atgttttacc aagagccggc ctaattcgat gcgcggcacc gggcattgat gggccttttt
     2101 caaggattcg tagccgcctt ttccttggag cagtacatcg aggccgcccg aagagtcgcc
     2161 gaccgtatga gagacggcaa tgatgtcccc gtccttagcg ccgctgcgcc gcagggcttt
     2221 tccggcttcg atttcaccga aggcagcgac ggtgatgacg gcaccttctt tcgccatgac
     2281 cgtatcgccg ccgaggatat tgacttcata ctgccggcag atatccttga taccgcgata
     2341 cagttcgacg acatcatcga tttccatgga cgatgtcagt gccgtcgaca agacgacctg
     2401 cgtcgggacg gctcccatgg ctgcaatatc gctgagattg gacgctaccg atttaaagcc
     2461 gacgtcatac cactgggccg tctttccaat aatgaagtgg ctgttttgga ccatcgtatc
     2521 gatgaccgtt acctgctgcc agcccggcgt cgtccggtag acagcgccgt catcaccgat
     2581 gccgatgacg acattttccg gatgatataa ggtattttct ttgataatat
//
LOCUS       DAWDXZ010000235         2625 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7855_length_2625_cov_7.907393, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000235 DAWDXZ010000000
VERSION     DAWDXZ010000235.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7855_length_2625_cov_7.907393"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1925..1934
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          605 a          761 c          666 g          583 t
ORIGIN      
        1 caccgcccca ttttttcatg aagtcaatcg tttcatcgcc gaggggaacg ttcttcagtg
       61 ggttttcttt gagcttatcg gcttgttcac gagctttgtc cttccattcg cccatcattt
      121 cgatatacgg acccatgtcg gtaggcatgc cgtctttgag ccagcgctgg gtgacccggc
      181 ccaggctgta ggtaataccg acagcaacgg ggacctggat gatactgaaa ggaatgagcg
      241 tcgtcaacag cgtaccggcc acgcggctgc cgactgcccc taagaaggcg atcagggccc
      301 gttcgctcaa tttaacgtca tagactttgg caatacggct gaccaggtac acttcattgg
      361 ccatgagtgc tgccgttccg ataagcggcg caatgacgat ggcaccggca cgggcagcag
      421 cccagcggca cagcatttcg acttggacgt ctttgtcttc cgtcgtctgt tccatggctt
      481 tgacaacact gttggcagca tcttcaatcg gctgagaatg gctctgtttg aaatcagcca
      541 gttcctgttt caaagcggca atctcttcct gcaactgttc tacggttttt gcattttctt
      601 catttgccat acttaacacc ccattctttg acgaaataaa cactatattt tatattttcc
      661 catgtttcta ccagcttgtc aaacccccgt ctacgggaag tgccgcagcc gtaataaagg
      721 aggaatcttc actgagtaaa aaggcaatgg ccttagccac ttcatcggcc cgtgccaatc
      781 ggtataacgg gtattgttcc ttcatggtct cccgctccat atcgggatga tcccggagct
      841 gtttttcgag catcggcgtt tcgacgtcac cgggacagac gcagttgacc cgtatctggt
      901 gcggtgccgc ttccagggcc agggacttgg taaaagcgac aacagcggca aagagctgct
      961 gcacgtcgtc aggcgtcaca ttttcaagaa ggccttgttc gtagatgccg gctgacgtca
     1021 ccaagccgtc cagaccgcca aagcggcgga ccgtctcgat caccatcttc cggcagtctt
     1081 catcccggct gacatcacct tctatataat ggacggcccc ctttccctcc aactcctgca
     1141 aggcagcctg tcccctttcc cgattcctgc cgctgacagc gacctgccac ccccggtcca
     1201 aaaggacggc agccgtcgca agacctatac ccgacgtgcc gccgctgata agtgccgtat
     1261 tcatgataac ctcctcatag aacgccaaga taagaaaggc ccagctttcc gataagcgct
     1321 gccgtcacga cgagcatgac gacccgaaca aaaccggttc cccggcggat ggccaatcgt
     1381 gaaccgaaaa aggctcccgt aaagatgcac agggccatga cgatgccgta gacatagagg
     1441 atcgtcccgg accagaccaa gatgacgaag gccgtcacgt tgctcatgag attgagcatc
     1501 ttggcattgc cggcagccgt gacgaaatca aagcccaggg ctgcaaaggc aacgatgaga
     1561 aaggtccccg tcccggggcc ggcaaagccg tcataaaatc cgatagcccc ggcaaagagg
     1621 gccgtaaaga gcaaatttct ccccgtctca ccggcatagg tattgaccga tcccaggtta
     1681 cgccgcgtaa agacgacgac ggcggccgcc atgagcgaca ggataatcaa cggctgaagc
     1741 cattcggcac tcatgtgcag catgaccaga caacccaaaa cggcccctgc aaaggtgcag
     1801 ggcaaagccc gtttcatcaa gtgccggtcg actttgccgg ccttaaagaa ttgccaagca
     1861 ctcataacgg ccccaaaggt ggctgaaaac ttattggtcc ccagagccag gtgcggcgga
     1921 aggcnnnnnn nnnnccgtat ctgtcgccgg ctgcaagagg cggacgattt ccttgccgat
     1981 gatccgtgcg ccgtcacgat ggcagtcgag gctggctttc gacatcgctt ccaatttttc
     2041 cggatgggta aataagtccg atacgatgga aaccagttct tcttgtttct tcacccaagc
     2101 tgcacagccc ttggcctgca tatacttggc attggcctct tcctgacccg gaatcgggct
     2161 gtataggacc atgggcactt ggatagcagc gccctccgta caagtaagtg ctccgggctt
     2221 ggttaagagc agggacgccc ggcacatgag ttttgaaatc tggttcgtat aaccgaggat
     2281 ttccacatca tgatcgagtt cgccgcgcat cttcgacagt tcatcgtaaa gatcggcatt
     2341 ataaccggtt acaacggtaa agctgtcgac gacggataac cgatccagca gtgccaggga
     2401 ttgtcggata gaccccattc ccagaccgcc gcccatgata aggatattct tattattctg
     2461 atgctcctca tcccagtgcc agcgttcttg gtcaaaagcc ttccgcacgg gaatgcccga
     2521 cacgacaatt ttatcagcag cgacgccttg ttcgacgagc aggtctttaa tctgtgtagc
     2581 tgcgacgcag tagacgtcga tttgcgggta cacccacagc tgatg
//
LOCUS       DAWDXZ010000236         2622 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7866_length_2622_cov_6.751850, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000236 DAWDXZ010000000
VERSION     DAWDXZ010000236.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7866_length_2622_cov_6.751850"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1351..1424
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          623 a          628 c          467 g          830 t
ORIGIN      
        1 gacccgtcaa aggcacgaaa aaagcaccac ccataaagga tagtgctttc tcccgtatac
       61 tttgtttcaa tccgcgcccc cgaaggggcg acatacgtgc tgttagcttt tatcgctaac
      121 cacaacatat atctttcaat ccacacagcc ctcgcggact gtgactacat ctcagagaga
      181 tgcctttact taaacatatt ataacacatt caatcgtaaa aagccatgtg ttcttccaca
      241 actttctttg tttcggcttc catatcgtcc aatactttct tgggatcttc tccgttaatt
      301 acccgctcaa ggcaacgttt tccaatcatg gccatgctgg aattgctccg atacgattgt
      361 tcttctgctt tcaaataagc atatgcgcgt ggatacttac gataagcggc cgtaaaatcg
      421 tacttaggct tcggccccac tccgacccca ccggcatcgt caaaagattt attccactca
      481 tcgtgccact tttctctttc atagatagcg ttttgtattt cttttaggcc tgggatagct
      541 gcaatcttag cttttcgttc ctcagcagct cttttccgtt catcttcaat ttctgttagc
      601 cttgcgtgaa tcttgggttt aagtttttta aggtcttcaa atgtcccatc ccttttgacc
      661 cggtctgggt tatgaaggaa tattttatca ggcatatcat aagggataat gatacgatat
      721 cggtcaatga tttcatcaac agacaactct gttaattcca ttactgtccc ttttgctttg
      781 atttttgtca taacaatacc actcctttac atgaacggat cgttaatttt atcgataata
      841 tctcccagtt tgtatttttt atatagttct ttcagccgtt tttggtattc atcttcgccg
      901 aagggtggtt cttccaattg taatctgacg atctcgatta ctcgttgact cgtaacgtag
      961 ggaatctcca ctagtctcaa gccaactact ttgtttagtg cataatcgat gagaatcccg
     1021 tggagtaaca ttcgcccgcc ttctggaatg tcacgcatgg cttctagggg caagtcccca
     1081 tccatgttcg cgtgatatgg ggccgctacc catgggccac gcccgaattg gaatgcgggg
     1141 aaggcaactc cttcgacgta agccattcca aaattaaatt gcccattatt aaaatcgtcg
     1201 attattggct ggattattcc cggcatgtta acaatcaagg ttggtacggg attaagtgta
     1261 aagacaatac tttcttcaat ttttccacct gggtactgct catttaaccg taccttttcc
     1321 agaatttcag gcgttacttt ttgcatcatt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtttaa aaatccactc
     1441 attgtatctc cgttttcgcg aagaattctt atcagctctt tttttacatc cgcttcacat
     1501 ctaaaccgaa tataatccgt cttcacaaca ttcctattgt catccatatt atcactcctt
     1561 agcatcattg taatgattta attataacat tgttacaatg atgctatcaa gttgttaggc
     1621 atcggagttc ctaaaattgt tattaatacc gggcgggtaa ttagctcgcc cggctaaatc
     1681 gttctattcc tcgtcgtcga atactacttc gctgatatgg aatcccttac catcgggacc
     1741 ataaaagtat tcagctttgt atcctttttc gacatattct ttttcgtaat cggtgggctc
     1801 catgtcaagc agctccatcg cttcgtctac gctaagcatc ctgttcgtcc aaatctttcc
     1861 caataccaat ccggaatgca attttactac tacgcgtctt tccatgatgt cgtcctcctt
     1921 tttattggca ccgggcgagc tagtttcccg cccggcatgt tagattttac caaatgtcgt
     1981 cgaaaggatc gtgttttacg cacaccttag gatcagggcc gttaccaacg acctcaaagt
     2041 ttttcccaag aatccattcg attttcttcc gggtgatttc tcccgcgacc atattattct
     2101 ggtcgtcaat atagtgttca tcaacccagg cgtcccgttc tgccttgcta tcgaattgat
     2161 agaagctgta agcattaccg tccataattc cgtagctgtg gcggagcccg aaaggacaat
     2221 attctgcgta ataatgtttt ttagccattt tagtttcctc cttaatttac acttatctgg
     2281 agcttccgtt tcccgctccc ttatcttgat tacattataa cattgttata acgatgtgtc
     2341 aatagataaa tgagctattt ttgaaaaaaa tcaaaaaaat aagccccgca gaatcggcgt
     2401 aaaataccga cttgcgaggc tctcttataa tactatatta ttgtttaggc tgctggacac
     2461 tcttattgag tgccaacttg gccgcccata agcatccgga gatgataagc ggcaaaatga
     2521 cttgatcgcg taccttggtc cagccgcgtt catccttggc ctgagcctgg atctgcccga
     2581 caaacttgtt ggcgacggcc tcgatggccg gcagcccgtt at
//
LOCUS       DAWDXZ010000237         2606 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7914_length_2606_cov_7.693454, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000237 DAWDXZ010000000
VERSION     DAWDXZ010000237.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7914_length_2606_cov_7.693454"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1744..1753
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          617 a          661 c          686 g          632 t
ORIGIN      
        1 tgatacttca tctatcccct tccataaaga ggctattgat atggccgcta tgggctttat
       61 cccggccggg gcgtaccgta accgtgattt tgcggcaggc cgcgtttcag ccgacggcat
      121 cgataaagga atgatggatg ttttatatga tcctcagacg tcgggcggtc ttctcattgc
      181 cgtttctgaa aaagatgccg tgcttttgga acggtccttg aaggacgtcc ttccttgtgc
      241 cgctgtcatc ggctatgtga ctgcgcggga aggagaatcc attgtgattg aataaaactt
      301 gcaaaacggc taccttcggc actatagata gtgtataata gtgacggtga gtttttttta
      361 ccgtactcat gtcaatgata aaggagagat caactgtgat tgaacgctat acgaaaccgg
      421 aaatgggtca aatttggaca gaattcaatg aatgggacac gctgaaacag gtggaaatcc
      481 tggccagtga agcccaggct gaacttggga atatcccgaa agaagcggct aaagaaattc
      541 gcgaaaaagc tgccttttct gtcgaccgca tccatgaaat cgaagcggaa acacatcatg
      601 atattgctgc tttcgtctcc aacctttccg aaaatatcgg cgaagccgga aagtacgtcc
      661 atttgggcct gacgtcgaca gacgtcaaag atacggcttt gggttccatg ctgaaacagg
      721 cttcggatat cctcatcaag gacctcgaag acttccacgc cgtattgcgt cgccgtgccg
      781 ttgaatttaa atatacgccg tgcatcggcc gtactcatgg cattcacgcc gaagcgacga
      841 ctttcggcct caagctgtgc aactggatgg ctgaaatcga acgcgatatc gaccgcatga
      901 aacatgccaa ggaaacgatt gccgtcggta aaatttccgg tgctgtcggt acgtatgctg
      961 acgttgatcc cttcgttgaa caatacgtct gcgaacacct gggcattgcg gcatcgccta
     1021 tttcgtcgca gaccctgcag cgtgaccgcc acgccgaatt ccttacgact ttggctgtca
     1081 tcgccagctc catcgacaaa atggcaacgg aaatccgtca tctccagcgt acggaagttc
     1141 gcgaagcaga agaatacttc agtcctaaac agaagggctc gtccatcatg cctcataagc
     1201 gcaatcctat tacttgtgaa cgcatgtgtg gcttggctcg tgtcatccgc ggcaatgccc
     1261 aggcggcttt ggaagacgtc gctctctggc atgaacgcga tatttcccat tcttccgtag
     1321 aacgggttat cctgccggac agcaccattg ctcttgacta tatgctccag acctttaccc
     1381 gggtcatcga caagctcctc gtctatcccg ataagatgat ggccgacctc aatttgacgg
     1441 gcggactcat ttacagcccg atcatcctca ataccctcgt cgaaaaaggc gccgtccgcg
     1501 aacaggcgta ccgctgggta cagcgcaatg ccatgaagcg ctggatcgac ggagaagact
     1561 tcttggaaaa cttgaagaaa gatgaagacg ttaagcagta tatgaccgaa gcagaaattg
     1621 aaggctgctt tgatgtcaag aaaatgctca gccacgtcga taccatcttt gcacgtttcg
     1681 gcatttaagt tgtaaccttg taagggcctt acgacctgct ttggcagtct cgtcgggcct
     1741 tttnnnnnnn nnnccaaaag ccggaagccg tatcgaaggc cgtctttgaa gccttgacgg
     1801 ccggcgatat cggcaatccg tcccgcggtg cccacggcta ttctttaaag gcctatgaca
     1861 tcgtcttgac ggcgaaacat cgcgttcaga cactgtttaa cggtaaggat ttgtacgacg
     1921 ttgcctttac ccacaattct acggttgcct taaatatggt gctgaaaggg ctgatccggc
     1981 ccggagacgg cgttatgacg acggcttggg agcataatgc cgttctgcgc ccgttatacc
     2041 agttagaggc tttaggggcg gaactgaccg tcattccggc tgtcagtcct acaggagggc
     2101 tggcttacga tgacatggaa gggctgctga cgccgaagac caaatggttg gtctgtaatc
     2161 atgcctctaa tgtgacgggc aatgtcctcg acttgaaccg catcaaggct ttttgcagga
     2221 atcactgttt ggggctcatt gtcgacgtat ctcaaacggc aggggcttat gacatcgatt
     2281 tgtctgacgg catcgtaacg gcggcctgct ttaccgggca taaatcgctt tatggaccgg
     2341 gcggaacggg aggaatcgtc atccgccgcg gcgtcgaagt cctgccgtct attaccggcg
     2401 gcgacggcgt tcatactttt gagcgggagc agccttcagg agttcctgct gtttttgaag
     2461 cggggacagc caatgtcgcc ggcattgccg gcctgagtgc tgctgtcgaa cttatcttaa
     2521 aaaaggggat tccggctgtc agggctcatc aagaggaatt ggcccgagcc tttattccgg
     2581 ctgtgaaggc cattcccgga atttgc
//
LOCUS       DAWDXZ010000238         2598 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7937_length_2598_cov_10.517892, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000238 DAWDXZ010000000
VERSION     DAWDXZ010000238.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7937_length_2598_cov_10.517892"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          534 a          659 c          782 g          623 t
ORIGIN      
        1 gaatgcgctt tttttgaggg agtacatcat gcgtttaggg attatcggtg ccggcagagt
       61 cggcacttct tttgttttag ccatgccttc ggccgttgaa ggtattcttt gttctacaaa
      121 agaacatacg acgcagcggg cgcagtccct tcaggtcaag gcctattccg acggggccga
      181 tcttcttcgg cactgtgatg tcctgctgct gacggtacgt gacgatgtga tcgggagcgt
      241 tgccggggcg ctggcagagg ctctttccgg ggatgacggg gcttgtgata cggttatcct
      301 tcactgcagc ggggccatgg atttgtcttc tcttgagccg ttgtcggcct tgggattttc
      361 ggtgggcagc cttcatccgc tgcagagctt tgccgcaccg tcgggagatt tgctgcgcca
      421 tatttatatg gctgtcgacg gcgatgaccg ggccaaggaa gcggcccttt cgctggtgac
      481 gtctcttgac agcaagcctc tttttgtgcc gccggaagaa cgggtcctct accacgccgc
      541 tgcttgtttt tgctccaatt acgtcgtgac ggctgtcgct ctcgggcagc ggctcatgcg
      601 gcgctggacg gagacggaca aggaggctgc acagtcgctg atgcctctcc ttaaggggac
      661 ggtaggaaac ctcgaaggca ccgatcgctg gcagaaggcc ttgaccgggc ctgtttctcg
      721 cggcgatacg gggacggtgg ccaaacacct ggaagccatg ccggccgatt tggtggggcc
      781 ttattgtgcc tttagccggg ccgcgggaga actctcttta gaaaacggaa cgattacgga
      841 aaaccagtat gaagtactga aagaaatcct tgcgatggcg gaaggagttc acaatgaata
      901 agaaagtaac gaatctgacc atcaagaaaa tgaaagggga agggacgccc atttccatga
      961 ttacggctta tgactatgtc atggcgcaga acgtcgacga agccggtatc gacatgatcc
     1021 tcgtcggtga ttctttgggc aatgtcgtca tgggtcatac gtcgaccctg ccggtcacta
     1081 tggacgatat ggtccatcat acggcagctg tcgtccgcgg gacgaagcgg gctttggtcg
     1141 tcgccgacat gcctttcatg agctatcagg aaagtgtctg ccaggccctt cacaatgccg
     1201 gacggttgat gaaggaaacg ggctgtgatg ccgtaaaatt agaaggcggc gccagtgtct
     1261 gtgaagctgt caaagccatg acgacggcag gcattcccgt cgtcggccat atcggcctta
     1321 cgccgcagtc ggtccatcag ctcggcggct accgcgtgca ggggaaaaat gccgaaacgg
     1381 cgaaacgctt aattcaggaa gccttggccc tagaagaagc cggtgccttt gccatcgtct
     1441 tggaatgcgt accgacggat attgcagcgg aaattacgaa ggccttgaaa caggcggcca
     1501 ctatcgggat cggtgccggc aatgtctgcg acggtcaggt cttggtttgc acggacctcc
     1561 tcggcatgag cggcggtttc cgtccgaagt tcgtcaagac ctatgccgac ctccatcaga
     1621 ccatcgtcga tgccgtcggt cagtacatca aagacgtaca ggatcggagt ttccccaatg
     1681 aggaatatag ttttgctgtc gatccggaag tcgtcaaagc catgaaggag tagtcgattt
     1741 tacaattgct gagaaagcct tgtcttttct gacagggctt tttttgcgtt ttggagatgg
     1801 gggagaggcc cttgcgatac ttgcataaag cggctttggg ccgttgattg cgattgactt
     1861 gcgtatctaa tcattgtaca attaattgaa gaaacggaag cgaataactg aggtcgtttg
     1921 tccgaacgca cgcgtaccgc ctgcccgatg aaaggggagt actgcggcgt ttttttctta
     1981 atcgacctgt atattattag gaggctatgc gttcatgagg catgatctac gcgacgtctg
     2041 tatgcagata cggcgggata ccgtgcggtc tatttaccgt gccgggtcgg ggcacccggg
     2101 cagctcttta tcggctgtcg aaattatggc cacgctctat tttaccgata tatttacgtg
     2161 ttttcccgac aggcctcatg cgccggaacg ggatcgtttc gtcttgtcga agggccatgc
     2221 ggcgccggct ttttacagca ccctggccca taagggctat ttccccttgg aatggctcga
     2281 tacgctgcgg accgtaggaa gtccccttca gggccatcct aacctgcaga agctgccctg
     2341 tctcgactgt gccgtcggtt ctttggggca gggcctgtcc attgccaacg gcatggcttt
     2401 gggcttgaag cgaaaggggc agatgcagca ggtattctgt ctcatcggcg acggcgagca
     2461 gcaggagggc cagatctggg aagcggcagc ctttgccggc cagcaccatt tggacaacgt
     2521 ctgtctcatc gtcgactgca acggcttgca gctcgtcgat tccatggccg gcatcaagga
     2581 tatggagcct atggatgc
//
LOCUS       DAWDXZ010000239         2586 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7958_length_2586_cov_8.338996, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000239 DAWDXZ010000000
VERSION     DAWDXZ010000239.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7958_length_2586_cov_8.338996"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1475..1484
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          682 a          680 c          627 g          587 t
ORIGIN      
        1 taaggaaaca cgctatcgtc agcgttatct cgacctcatc gtcaatccgg aagttacaga
       61 taccttcgtt aagcgttcga aaatcatcaa gggcatccgc aagtacctcg atgaccgcaa
      121 cttcctcgaa gttgaaacgc cgatgctcca taccattgcc ggcggtgccg cagcccgtcc
      181 gtttaagacg catcacaacg ccctcgatat ggatatcttc ctgcgcattg cgccggaact
      241 ccacctcaaa cgcctcctcg tcggcggctt ggaacgggtt tttgaaatga accgctgctt
      301 ccgtaatgaa ggcatcgata cccgccataa tccggaattt acgacggtcg aattgtacca
      361 ggcttatggc gatttggaag acgtcatcaa cattacggaa gacatggtct cgaaattggc
      421 cgatatgctc tacggtacga tgaagttgac ctatatcgat aaagaaattg acttaactcc
      481 gccttgggac cgtatgacca tgatcgaagc cgttaagaaa ttcagcggtg ccgattttga
      541 aaacgttacc gacgtcgaag tcgcccgcaa aatggccgat gaacatcacg tcgaatacgg
      601 taagtatgat ggtgtcggca agatcatcaa cgctttcttt gaagcctttg ttgaagaaaa
      661 cctcattcag ccgacgatca ttaccggcca tcccttggaa atctcgccgc ttaccaaaca
      721 ggaccaggat aacccgcttc ttacctatcg ttttgaagcc tttatctacg gccgcgaact
      781 ggcgaacggc ttctcggaat tgaatgaccc tatcgaccag cgcaaccgct tcatcgaaca
      841 gatgaaggaa cgcgaacacg gcgatgacga agcccatcag atggatgaag attactgccg
      901 cgctttggaa tacggcttgc cgccgacagg gggccttggc atcggcatcg accgcttagt
      961 catgttcttg accaacagtc cgtctatccg cgacgtactg ctcttcccct tgagccgtcc
     1021 ggaacagcat aaataatttt tgcacccttt gcccatgtca atggcaagaa aagggctgtc
     1081 gtaggacagc ccttttttat cacaacccat aaaaaggagg aacgctatga aacgtaccgc
     1141 attagctgca ttggtctgct ctgcttttct agcttgcaat tcggcagccg gtgctgtgtc
     1201 gacagagaac tgggaaagct ctctgaaaaa tgcgccgaca tcccttgcag cagcgattgg
     1261 ggaagaactt cttctcgata atgccgatgg tgccgataaa acggcatcgc ttgaaaagcc
     1321 gtcgaagtca gccgaaccga aagagtccgc caaaaaagac gcagctaaaa agtcggcaaa
     1381 atccgataag gttcagccgt cttctaccgc caaagagaaa acggaaccgg acaaggctga
     1441 tgatgctaaa aaagacgatg ccaaagacgg taaannnnnn nnnnaaaaaa gccggttatc
     1501 acaacgctgc accagccagt gtccacgctt tgacgtcaga cgatcagcgc gtcttcgatt
     1561 cgctggatac gttgaagatt gcccccttag aagccggtga cttttacatt ggcagcatcc
     1621 acagcggtga cagtccgatg aaggtgaagc ggattttcgg caatcccagt aaatactcga
     1681 ccagcatgca ctacacgtcg atgcagtata cgggaaaaga tcttaagctg cgctttgtca
     1741 ttcgcaataa attggccgac agcctgcgct ccagcggcga ctcgcgaaaa agcgtccgtc
     1801 ccggtgtcga gtctgccttt ttgacgacgg ggaccaatat cctcatcggc cgtgacatgc
     1861 gtttgaagta tcccattgaa gtgcttttgc gccaattcgg ccttcccgat tccgtgttgc
     1921 gcgatactga tgccaacgtc tattacgtga cctatgaaag ccccaaaaag gatgccatgt
     1981 acgtctttgc tgtcggcaat cgacgcattg aacgagtggc cctcatgccg gttcgtccgc
     2041 cttatattac cggtgagccg tcgaaggatc cgatgaagtt gaccgagaag gacttcaccc
     2101 tcatgggttt tggcctcaac caaccgtttg aagccaataa gtacaatatg tggaacaaac
     2161 tggtcaagcg caacaacagc agcttttggc tctacggtga ctatggtgtc gaagtagacc
     2221 ggcgcaatat ggtgcagaag gtattccttc tgaccaacaa tggctatacc agccgcggtg
     2281 cgaccctggg ctatcacgta tcgaccatcc tggcgctata cggccgtccg gaccgcgtcg
     2341 aatggggccc ggataaggaa aagtccgtcg acgcctatta ttatgacagc ccttaccaaa
     2401 aaggcgtatc cctggtcatc atcatgcgcc acaatgagcc ttatgtcgac gatgtcatcc
     2461 tgacgtcgac gccgattaag aatatccaag acccgatgga gcgttacgga ttgaagtagg
     2521 gatgcgtacc gttttggcat atgtgtcaat aaaatagtaa aaatgcttta cttatatgat
     2581 gaaagt
//
LOCUS       DAWDXZ010000240         2585 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7964_length_2585_cov_5.218972, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000240 DAWDXZ010000000
VERSION     DAWDXZ010000240.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7964_length_2585_cov_5.218972"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          649 a          661 c          633 g          642 t
ORIGIN      
        1 gtccatccat gccttacgtc atacctacgc cactaggcta ttagctaatg gcgtggacat
       61 aaaaacagtg tccgccctgt tgggagacac tgttaatacg gtccttaatg tgtatgtcga
      121 ttacacggac gacatgcggg aaaaggcggc tgaaagcgtg aaggaaatct tcggatgatt
      181 tttgacgaat ttttgacgat tgaataaaaa accgcataac taagccaatt attagctata
      241 tagagatatc tctatattat acaaaaaaac aggtcccttg tcagtaaaaa aagggctatc
      301 gcaggtgcga cacccctttt actcactggc tgctgcctaa tcctttgttc ctcggcaaat
      361 accgcggtcg gcattgcgaa ggaagcggag aaaatgttct acttcttcgg aactgtccag
      421 ttcctgttcc atttcaaaaa tagcctggcg caaattgagt ccggaacggt acaagatgcg
      481 gaatgctttt ttcaattccc ggcggacatc ttcgggaact ccggcccgtg aaaggccgac
      541 actgttaagg ccgatgcagc gtgcaggctg accgtcggcg ataacgaagg gcggaatgtc
      601 ctgtacgact ttcgccaggc cgccgatcat gctgttgcgg ccgattttga caaactgatg
      661 gacaccggca atgccgccga tgacgacccg atcttcaacg gtaacgtgac cggcaaggcc
      721 ggcgcagtta ctcatgatga cgtggttgcc gacgacgcag ttgtgggcaa catgggtaca
      781 ggcctggaac aggcagtcat tgccgattcg cgtttcttcc ccttcacctg tcgccgtgct
      841 gaccgtcacg aattcccgga aaaccgagcg gtcgccgatg ataagaaagc tcttttcacc
      901 gtgatatttt aagtcctgcg gttccgaacc gatagaacag ctcgggaaaa accggcagtc
      961 tttaccaatc gtcgtccaac cgtcaatcgt aacgtgggcc atgatttctg ttccgtcacc
     1021 aatggtgaca tgaggcccga taacggcata cggtccgatt ttaacaccag ccccaatttt
     1081 cgcctgcggg tctataacag ccgtcggatg tatcatgctc tgacgcatgt tattcgtttc
     1141 ctcggtcatt aaagtcactc ctcgcttccc tgtccctaaa agggacagtc gcttaaattc
     1201 ataaatatct acgtatatag tcgcaggaag gacgatttat ttcgcccggc aaaatcaagc
     1261 catttcccgc aatattcccc tttatgctgc taaatacgcc cgtttagctc attatttaac
     1321 ataaagtcaa ttttattgat tactttttcg gattctgtcc cggcatgagg gcaaagagat
     1381 attctccttc ggcgcagagg atatcaccga cgtgacactg agccttgacc ttgcccatac
     1441 ggccctttac cttttcgatg tcagcccgca tgaccaactg gtcaccgggg atgacgggat
     1501 ggcggaagcg gattttatcg attcccgtaa agaagggaat gaggccgcgg ttttcttcag
     1561 gatacaagag ggcaataccg ccgacctggg ccatagcttc ggcaatgagg acgcccggca
     1621 tgaccggctt gcccggaaag tggccttgga aaaattgctc attaaaggtc gcattcttga
     1681 tgccgatggc atatttcatc ggttccaatt caatgatccg gtcaataagc agcatgggat
     1741 agcgatgcgg caaaatttcc ataatatcag ttacttccaa agtcatgaga attcctcctt
     1801 agctcactgt agttcttcaa aaatctgatg tgtcagctgg ttgtttaagt tatggctcga
     1861 tttgacggca atgacatggc ctcgtatcgg tcttccgagt aaagacaaat caccgacgat
     1921 gtcgagcacc ttatggcgga ccaattcgtc atcggagcgc ggaacggaca ggcacgacgt
     1981 cttatcatag acgacgacat tttcaatcgt accgcccttg ccaaggccca atttcttcag
     2041 catttccaat tcttccgtaa aggcgatcgt ccgggcaaag gaaatctgct caatgtacga
     2101 agccttggtg acggtctcgt caaaaaactg gacgcccagc atagggtgcg gattgataga
     2161 tgtaaaggta atgcggaatc catcgtaagg cacaagagcg ataaacttat ctccgtcctg
     2221 aatgagatgc tgtttctgta tgaccagcgg tcggcgcggc acatcgagct cacggactcc
     2281 cgcttcttca atgagcttga caaaggtcag actgctgccg tcggcaacgg gcggttcgac
     2341 ggaatccatt tcgacaaagc agttgtcgat ttccatccca taaagggccg acagcagatg
     2401 ttctaccgta aagaccttgg cgctcccttt ttccagggtc gtcgcccgca tcgtattggt
     2461 tacattcgtc agatgggctt gtacagacgg tttttccggc aaatcggtcc gaataaaaac
     2521 aatccctgta tcagccggag ccgggcgcag taccatatga acttcgttgg cagcatgcag
     2581 gccga
//
LOCUS       DAWDXZ010000241         2574 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_7991_length_2574_cov_10.477967, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000241 DAWDXZ010000000
VERSION     DAWDXZ010000241.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7991_length_2574_cov_10.477967"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          587 a          707 c          654 g          626 t
ORIGIN      
        1 gccctggcct gtaaaatcga cggccagtac gataaggcca ttacgtactt taaaaatgtc
       61 atctcagccg acccgggaaa cacgtggagt tattacggca ttgcgacaat ttacggcgac
      121 ctgggaaaag cagaccaagc cctgccttat ttaaaacaag ccattgacct cggcggtgaa
      181 tccgtaaagg aggctgcccg gacccagagt catttcgata agatcagaga caacaagcag
      241 ttccaaagcg tcgtccgtta attacatatc ttcttcagcg atttcacaaa gaatatggcc
      301 gatcattata tgcatttctt gagcccgtgc cgttacctta gccggaatgg ccaggcagat
      361 gtcgcaggca tctttcatct taccgccgcc ataggccgtc atgccgataa ccgtaaggcc
      421 tttcgcatgg gcttcttcaa tggcttcgag gacgttgccg ctgttgccgg acgtcgaaat
      481 accgatcagg atatctcctt tttcaccaag ggcatggacc tgacgacgga ataccgtatc
      541 aaagccgtag tcatttccga cggccgtcaa gatcgacgtg tcgacggtca gggcgatagc
      601 cgggaaggcc ggccgttctt tctgaaaacg accgacgatt tcagcagcca agtgctgcga
      661 gtcggcggcg ctgccgccat tgccgcaaaa gaggattttg tggcctgaag ccagggcttc
      721 tttaatgaga gccccggctt tttcaatatc gggaattaat gctttcgtcg cttctaagac
      781 agcggcgtgt tcttccattc tttgggttac taaactcata aaacctccta tttccggtct
      841 tctccatcgt cgacaacacg gatttcgccc gtatgaggat ggatgaccat tccataaatc
      901 gtaagggatt tcggtaagta gggattcgcc cgcagtcggc gcaccgtatc acagaccgac
      961 gtatcgacgt ccgtaaccgg gtcagcccac tgcttaagct gcggtcggat agcgtcaatg
     1021 gccgtttcat cgatgccttc ttccaccatg tggcggcaca ggctgtcggc cgtcgtatgg
     1081 agcatgccgc agtcatcatg acccatgacg ataatttcat ggacatggag ttcgtaaacg
     1141 gcaaccatca gactgccgat gacactgtcg aagtcttcaa aggccgtatt gccggcgact
     1201 ttgatgattt tggcatcacc gcggacgagg cccatagccg gttccaaaaa atttaaaagg
     1261 cgtgtgtcca tacaagtcaa cacagccagg tggcggcgcg gatatttgct gacgtcgtcg
     1321 aaacctcctt gtgacaagga ctgtttaaca aacttttcat tcgtattaag aatctgacga
     1381 atcaactcac tcatcctatt tgccccctaa gcttttacag cttccattac ttttttagcc
     1441 tttaaatact ggtcgatgcc ctctgccgct ttgcgtcctt cgcgcatagc taaaacgacg
     1501 gtctgcggtt tgtggacgat atcaccggca gcaaagacgc cttccttgct ggtcatgccg
     1561 tacggcgtat ccttgacggc gatatagccg tcgtcattgg aagaaatgcc gaaggacttg
     1621 atgaccggaa gttccggctt attaccgata gctgcgatga tcttattggc cggtacgaca
     1681 ccgaattctc ccgtcggcac catggtagcg tcttcgagga tctgctgccg ttcataacga
     1741 agaccttcca cacggccttc tccaaggaca gccttcggtg ccgaataaaa ttggaagcgg
     1801 acaccgtcgg ctttcgcttc ttcatattca gacggcaggc atttcatatt tcgttccgcc
     1861 cggcggtaaa cgatggtaac gtcggcgccg agacgaaggg acgtgcgggc cgcatcgatg
     1921 gccacattgc cggcaccgat gacgatgacc ttatcgcctg cccggacggg cagtttttcc
     1981 gggtcaaatt cgccgtcctg agccagttgt acttgacgca ggaacggttc tgcttcgacg
     2041 ataccttcca catcttcatt gtcaacgccg aggttccagc catccatcgt gccgacagcg
     2101 ataaagacgg catcaaagga cgatttcaat tcgtcaaggc tcttatcggt gccgatttta
     2161 acaccgtatt cgatggtaac gcccatgtcg ctcagggccc gggcttctcg ctgcaagagc
     2221 tctttatgaa ggcgaaaagt cgggataccg taggtcatgg taccgcccgg atgagaagcg
     2281 gcttcataga tggtgacatc gtagtcgcgc ttagccagtt cacgagccgc tgccataccg
     2341 gcaggaccgc agccgatgac ggcaacgttt ccggctgttt tcttgctgac cggctgatcc
     2401 ataaatttcc cttccagggc catgtctgct gcaaagcgtt cgagctttcc gatttcaacg
     2461 ggctgacctt ttttgccgag gatgcagtgg ccttcacact gcttttctct cggacagata
     2521 cggccgcaaa tggccggcaa atcactgcgg ttataaatat gcgcagcagc tgcg
//
LOCUS       DAWDXZ010000242         2569 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8005_length_2569_cov_11.375895, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000242 DAWDXZ010000000
VERSION     DAWDXZ010000242.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8005_length_2569_cov_11.375895"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          685 a          626 c          624 g          634 t
ORIGIN      
        1 cgacgacctt ttccttcatc tggaatcccg tcatatggtc gtcatcggtc cagacgtact
       61 gttcgtggat gttattttct ttcagaaaat tgacgatgca gcggccaaag ggatcgatac
      121 cgaccttaga gatatacgat acattatttc ccaaacgagc cataccgacg gcatagttca
      181 gttcagcccc gcagacgtgg cgggaaaaat gttcgaccga ttccaacggc cccgtttctt
      241 cagcaatgag aagtcccatc gcttcaccaa tagtaagcac attcttcatt ttgattcctc
      301 catgagataa gatgggactg tcgcagcaga cagtccccgg ttacttagtt ttacaattgt
      361 ttctttattt tatcaatcat atcttcatag tctgcatctg tcaagtaaac gcggtcggga
      421 ttcatgacaa aggttccgtt ccattcgaat tcatctttat acttgggaac taagtggata
      481 tgcaagtgat gtcctgaatc ggcataagca ccatagttta ctttgtccgg atgaaaagct
      541 gcatgaatgg ccttcgaagc ccgtgcaacg tcagcaaaga aggcatttcg ttcttcatcg
      601 ctcaagtccg tcatttcgct tacatgatct ttataagcga cgatgcagcg gccgggatga
      661 ctctgctcct tgaataaaac caacatactg accgataaat caccgacgta gatgccaaac
      721 ttagccagcg gttcgccttt gacgcagtag ccgcagttct gatctttcat gtgaaagcct
      781 ccttattcaa tggtaccgtg ttcccattta ccgttattca aatcttcagc gatttgttta
      841 aatttatcat ccgtcaagcc gtagaagaag tacaggataa tcgcagcaat accaagggca
      901 acgccgggat acaaggtcat agcagctttg atgcccaaga gggtcatttc gctctgtacg
      961 gcattggcaa cgtaacccgt catgaccagg atgccggagc ttacgacagc ggccagagcc
     1021 tgagcgattt tgcgggagaa gttgaaagcc gcataggtga tgccttcttt acggataccg
     1081 ttgtgccatt cactgtagtc gatgacgtcg gagacaaagg cccaggtgac accgttagga
     1141 atagccaagg caacgtagcc gatggtaacc aaaacgatga acgagtaaat gttggtcgga
     1201 ataacgaagt tgagaaggtc tgccgcacag cctatgagca agccgccgat agccgtcttc
     1261 tttttgccga attttttaac cagtttcggg atgagcagga taccgataac ggagcagccc
     1321 atcatgatac cgtttacaat cggctggaag cccatgtaac ctaagttata ctgacagaag
     1381 aagatcatca tctggttatt ggtattcata gccgaaatcg taaacaaggt cattaaaatg
     1441 atgcataaga gcggtttgtt agtaaacacg agtttaacat agtcggaagc tttggctttc
     1501 tgttcattag ccgtattgcg cttaacagga acgtgttcac ggcagttaag atagcaaata
     1561 gcaaagccga tgacaccgac gatagacatg acagccgcgg cgataaagaa accgtggtga
     1621 ggcgaagcaa acatcatgac gatcgggacg aaagccatgc cggtgatgag ctgagcccct
     1681 atggaaccgg cctgacgggt cgtagccatc tgacttcgtt cttcaacgtc tcgagtcata
     1741 acactggcca gggaggaata gggaatatta gtaaacgagt aaacgagacc ccaagccatg
     1801 taagcaccgt aagcaaagat gattttacct gttgccgata tttccggcat ggtaaaagtg
     1861 atgaccgtta agatagccag gatgacggca gaaaacatca tgaccggacg gaacttgccg
     1921 cgaggaccga tgttcttgcg gccgtcgata acggagccgg caatcggatc cataaaggcg
     1981 tcgaagattt tggtaaaggc gaagataccg gcaaccgccg cagccgggat ggtgcaaacg
     2041 tcaatccagt atttcgtcaa ataggcctgt ccgaggtcaa acataaagcc gttgccaaag
     2101 tcaccaaacc cgtaacaaat cttttcacgc caggacagtt ttacatgacc gtctttggct
     2161 gtctgccctt caaccggagc gttcggcttc ggcaaggttc cttcttgtgt gctcattgca
     2221 ctccctcctt ataaggtaac accgttttta aatatagcca gttcgtgttt atcaagagct
     2281 tccgattttg ccaaaatacg tcccgacgct acatcgagga tgtattggaa caggcggtcc
     2341 gccgcagctt cccgcgattc cccttcagca atcgtgccgg cattgaaatc gatccagttc
     2401 tttttgaatt cggccagttt gctgttgctg gaaatcttaa tcgtcggtac cgggcaggca
     2461 aagggcgttc cccgccccgt cgtaaagagg accatgtgag caccggctgc ggctaaggca
     2521 ttggccgcta ccaagtcgtt gcccggagcc tgcaagaggt tcaggcctt
//
LOCUS       DAWDXZ010000243         2562 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8026_length_2562_cov_4.970483, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000243 DAWDXZ010000000
VERSION     DAWDXZ010000243.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8026_length_2562_cov_4.970483"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    281..366
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          686 a          536 c          653 g          601 t
ORIGIN      
        1 aacaaattga gatgaagatg agaacctatt tggaactgga tgaacagatt caatataacg
       61 gaaatctcat gatgcaaaaa atcatgtctc gtactcaaac gtgggtacag cacgatttct
      121 tatcgaatgc cgaacagacg atacgaaaca gaatcaatga ccagagtttc tttagcacaa
      181 cttcaaaaga tgcgattcaa cgagaactgg aacaactcct gtcagaagat cggcttcagc
      241 aggaaatcaa tggattttta agccaattac aaagtgatat nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnccaa gaaaatcaaa tcaaaaatga tttggagatt tcgcagctgc agcaggcctt
      421 tgccgggcag acggggatgg actcgatgaa agattcggtg ctggttgcca gcgctgccgg
      481 cggagcttta tcggtctatt cggcaatctt agggccggct gcatcgacgg taaccattgg
      541 ttctgccgtc ggggccatta tgccgcctat cctgctggca ggagctgccg tgggtctcgt
      601 ttccggctat gctaggagta aaaagctgaa aagcagtcaa accatgatgg ccaatgacgt
      661 gttagaccgg attcgcagcc acgtttcgca ggaattatta ccgtccttag aacaacatct
      721 gcagcaactg tgcagcaaga cggcagatga agccaagcgt gaattcgtag agaagaactt
      781 tgacggacgc agtgtagatg agttgaagga tttggttcag cagctgcagt cgtttttgaa
      841 acgagaagat ggggaagcgt ttgctaagct ttcgcaatat agcacatagg ggtatctttt
      901 ttgatttccg cgaaatcatc acatctctat gactgtgttg cagaaaagaa agtgaaacgt
      961 tgctgagata ccggccatgg atgtggcgtg cccgggcggt ctttatggga gcatgtgatg
     1021 aacgcccggg gacgaattcg ctgatttgac gtattggtaa ggaatgaggc agtattcgta
     1081 tgaggttgcc gtaaaaagga gtgagtcgat gaaacatatc atgctgctgt ttttaaacga
     1141 ggtccgtctt catcatgacc gtacgttggt tgtttcgcgt tataaaaata tctacgggac
     1201 gatgatcgac tgcatccagg acaacgaagc ggccattcgc cggacggccg atttattaaa
     1261 acggcaggga gaacaaatcg attatctgtt ctatctttca accaagggga cacaagaaga
     1321 gcttcgctat gtcgacgaag cgggtcatga gtgcagcctg acgcatgaag ccctctttcg
     1381 ggaacggata cagtccttgg cagctcattg tattcgcgtc gactacgatg aacactgtta
     1441 gaccggagac cgaatggggc aggcttttga aatggccggt accatacgtg cctgtatagc
     1501 agcccagcaa gggccgtctg aaggcgtaca gctccacgcg gatgttaccg gttcttttcg
     1561 tcatacagcg atgatactgg cagtcatgca gattttgaag cactatggat ttcgaacgac
     1621 ggctgtcctt gaggctgact gtcatgaacg gaaagtcgaa gatgtaaccg acatctgtcg
     1681 gctgttccac ctcctttcag ggactgatga attcatcaac ttcggcagta ctcgggacct
     1741 tatggcctgc atggaaggag atgaacagcc gccggaaatg aagcggatgc tgcaagaaat
     1801 gcatgaattt acccatgcca tacgcctttg ccggaccggg aaaattgcgc cgttggccag
     1861 aaaacttcgg gaagctttga agaactttga acaggccgga gccgtttccc gtcaggaaac
     1921 catgtttctg cgcatgttag aagtcttccg aggggagtac ggtttgcttt taaaggaaga
     1981 cgttacgaac ctcgacgtca tccgctggtg cgtcgaaaaa gactacctcc aacaggcact
     2041 gaccttgtgc agcgagtgga ttcccagtga ggtggtggat cggcatattt tttaccccat
     2101 taaaaaattt attcccaatc aatgtattcg ggaaaaattg cctcgccaaa cgtggcagca
     2161 gtattttttg aatgatctta ccgtaagacg gacgaaagaa gatagcgacg cctacttgaa
     2221 ccgcctattt ccccgattgg aaaaaaagaa gcgggaaacg aggcttcgca ccatcatcgc
     2281 cgactttgaa gcctcaggcg atgtggatga tgtcctgcgc cgatatccta aggaggcggc
     2341 ctatttcaaa gccttgctgg aagagttgcg ttttgtacgg cagatcctct ctgacttgac
     2401 gacggataag atgacgaagg aggaactgaa acagaagtgg ccgaaaatct atgccatttt
     2461 gcatcgcatg tatcatcgag tcaagaatag tcctaacttt gatctgactt tggacgaata
     2521 tttgaaaaat caaagggtat cggccatgta taatgttttg aa
//
LOCUS       DAWDXZ010000244         2561 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8027_length_2561_cov_9.009178, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000244 DAWDXZ010000000
VERSION     DAWDXZ010000244.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8027_length_2561_cov_9.009178"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          567 a          761 c          625 g          608 t
ORIGIN      
        1 cctcttctat gcttccatgg ggcttggcat gctgaccaag gggctcattg ccatcgcctt
       61 tcccgtgggc atcctctttt ggtatgccct gtgcacgcgg cggccgcggc tctttttaaa
      121 acttttctac gtgcccggaa tcctgctctt cctggcgatt tccgtccctt ggtactacct
      181 cgtatcgaaa gccaacccgg atttcttcta tttcttcttc atccgcgaac acttccttcg
      241 ctttgccacc aagatgcacg aacgcttcca tccttggtac tacttcgtcc cggtcctcat
      301 tgccggtctc atgccctgga cgggctttct ggcgaccttt ttcagtaaaa aaggcatctt
      361 ccgcgcgccg ggcacgctgc gtcacaaaca ggatctcatc ctcctgtcct tgtgggccgg
      421 cctgatctac gtcttttaca gcatttccga ttccaaactg ccgacgtata tcctgccctg
      481 ctggctgccc ttatcggtcc tcttggcagc ttccctggaa cggtgccgcc gcgaaaaatc
      541 ctggctcggc cacagtttcc tcatcaactc cctgctctgc ctcctcttta cggcagccgg
      601 cgtcgtctac ctcatgcaca ccgattttct gacgattgcc gacttcttca gtcacggcgg
      661 cctgctcatc gcctccctcc tcatcgggac ggcagccgca gcctggtcgt ggcacaagcg
      721 gcgaagcttc ctttcggtgt tcgtcatcct caccatcatg gcctatggct tcggcctcgg
      781 tgcccatcag attcaaggcc agatccacga ccaccagacg gcctatacgg tcagtcagga
      841 tattaaaaag ctgaacgtac cggacagtac gcccatcatc atgtacggct acttcatccc
      901 gggcctggtc tattacctcg accgtcccat tgccacgggc aacttcatgg gcgaattgaa
      961 cttcggcatc catcacacgg agcggacggg catgtactat ggcagcaacg aattgtatgc
     1021 cctctggaac agcgataagc cggccgtcat cgtcgtcgaa aagaaatact tggaagaagc
     1081 cctgcgcgtc gtcggaacac ctccggctcg gcgcattacc gacgaagact acacggttct
     1141 cttaaacggc cctgccgctc aggacgcggc tcgataaaga gctgtatcgc agcatcgata
     1201 cttgaaataa tccgctaaac aacaagatac acaaaaacca gcaccatgac gacaaccatc
     1261 gccatggtgc tggttttctt atcggtgaaa atcccgaggc aaggattcta tgtcagttgt
     1321 tctttgaaaa aatcggcaac cgcccgggct gccccgtctg tatcgtgact gaagacatgg
     1381 tcttcgccgt cgatgagatg gagttggccg ttaaagtaga tgcgctgata acgctggctg
     1441 tacgtataag gggcaatgac gtcacccgtg ccgtggatca tgtaggccgg gccctgatac
     1501 tgggacgccg tttcataaat gggaagggtc tgagccgttt tgacatagtt ggctccgacc
     1561 tgataatccc gtaccttgat ggtttccgac ggattgaggg gatcgtagtg aatcccaaaa
     1621 agctggccgc gaatggcatc ctcccgaagg acggcggccg gcgccatgag ggccagggct
     1681 ttcaccttct gtgtccccag ttcgccggcc gtcatactgg caacgacgcc gccttgagag
     1741 tgaccggcca gggcgattcc cgtcacgccg ggaagcttac cggcatagtc atagacggct
     1801 ttggtgtctt cgatttcgtt gaggacggtc atatcttgga atcgtccttc gctcttccca
     1861 tgaccgttaa aatcgaagcg gatggacgca atgccgtctt tttctaagtc atcggcgagt
     1921 ttcgtcagaa ggggccgctc cttattcccc atgaagccgt gcatgatgat gactaaagga
     1981 tattcgctct ttccgtcagg ccgctgaagg acagccgaca gttttccgtg atcgccgtca
     2041 atggtgacgt cttgggactg agcggcaccg acaccggtcc ccatagagaa gaggcccaag
     2101 gtcagtaaag aaaggcccaa cagtcgttta aaatgacgca taacaaaacc tcctaacgat
     2161 aggatactac catctgatat tgtattatca ttgtaccgcc tctccgcaat catgtcaata
     2221 attacatatt aaagataatg aaattccaat actgaaaaac gaccctccaa cgaagtacgc
     2281 cacacaccct caatgccgta tgccgatgga atccgtcgac atgtctcaat cccgcaacgc
     2341 cccaacgggc caagcgaatc gagaaaggat acgtgcaaga aagacactcc gtcacgaaaa
     2401 aagagagccg tctaaaaaga caactccctt ttgtatgtga atgacatcta gcgttaagcc
     2461 ggctcctcac ttctggcatg tcgggcagta atgggtgccg cggccgttta gttttacgta
     2521 ttcaatagtc gtgccgcaga ccgtacacgg cgtcccttcg c
//
LOCUS       DAWDXZ010000245         2524 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8133_length_2524_cov_7.293236, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000245 DAWDXZ010000000
VERSION     DAWDXZ010000245.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8133_length_2524_cov_7.293236"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    665..674
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1373..1382
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          622 a          693 c          590 g          599 t
ORIGIN      
        1 ttttgtatat ttttgagcaa tcgcgtataa taaaagcaag cattccaacc cattattgat
       61 tcaccagaag gaggtctttt ctttgtactt tcccaaactt gataaacagt ccttaactga
      121 aggcatcatc attcccgaaa tgataaaagt ccggcaaagc ttcccgtcac agaacctggc
      181 cgatcccatt ggccgattaa aggagcagct ccactctctc gatgagacaa ccattaaatc
      241 cttagaggga aaacgcatcg gcattacggc gggaagtcgc ggcctcccgc attataaaga
      301 gcttatgaag gtcctctgcg atcagctcaa ggactggaag gcaaccccct ttgtcttccc
      361 cgccatgggc agtcatgccg gagccacggc agaaggccaa aaagcccatc tggcccaatt
      421 cggaatcaac gaggagtacc tcggcgtccc ggtattatcg accatggacg tcgtcaccgt
      481 tgccaccttg gacgatggct ttccaattta ctgcgacaag aatgcctatg aagccgacgg
      541 catcattttg tttaacaaga ttaaacctca cacccacttt aaatacgagc acgaatcggg
      601 tctcttgaag atgatttgta tcggctgcgg gaaacacaaa ggggcttcgg ccttccacgg
      661 acgcnnnnnn nnnngacgcg gttacgatac ctttggtccc aacttggaac gggtagccaa
      721 ggtattctta aacaaggtaa acgtcgtctt ttccgtcggc atggttcaga gtcccagcga
      781 cgacatcgcc gccttggaag tcatccctac ggataaattt ttcgaacgag acgccgccct
      841 gcaggccctg gcaaaagaac aaatgccgcg cttgaagctg cctcacatcg atgtcctgat
      901 tgtcgatgaa atcggcaagg aaatcagcgg caccgggatg gatcccaaca tcaccggacg
      961 gacggaacga ttcagccaag aagaagcctt tcgttcactg gctccggaca tcgaacgaat
     1021 cgtcatcctc gacattactg atgcctctca cggaaacggc tcgggctgcg gcatggctga
     1081 catcgtcagt taccgtttcg tcaacaagat ggattttgcc tctgtttaca ccaattgcat
     1141 tacggccaaa tcattccgca tggccttgct gcctctttac ggcaacagcg acaaagatgc
     1201 catcgaaatg gccatggccc atgccttcct gacggacccc aataaagcca aggtcgtgcg
     1261 cattaagaat accctgaagc tggaagaaat cgagtgctcc ctatcctgcc tggaagatat
     1321 cgaaggccgg tccgatatga ccgtcatctc caagccgttt acttggacgt ttnnnnnnnn
     1381 nntcttatta taacccttta tggccgactt tatccatcca ggcgaagagt tctgccaggt
     1441 cataatcacc gccgtggcct tgtccccaag gagaagcaaa gtctacttca tatccggcct
     1501 gctgcagcct gagtgcaagg acagccggaa cggcaatagc cgtgtcccga tcggcttcgc
     1561 cgtgacggat acgccaatat ttagccgtct gaacatggga cttgccgata taattcaatg
     1621 ggttaagcaa ttttacgatg gccttatccg ccgtcgtggc cgtcgataag gaatgagttt
     1681 ccccgtaatc cgtaaagtgt tgtttatcaa tcgatgccgt cccaaattcc tgattttctc
     1741 cggagctcaa gtccaaagcg tcgaaagccg gagccgcctt taaccgcgtg acgtcacggg
     1801 cataaccggc taaatccata tcgacaacct tcccgtcttt aatcgtcagc cacgatttag
     1861 atgataaatc gattccctgg tccagcgccg tctgagcaga cgccatgtaa atcgatttaa
     1921 tgtattctgc aaaggacccc tgtccatttt catcaagggt caatgctttt ccgtctttat
     1981 cgacaaggtg cagtccgtta atgtaatcgg ggtaggctgc tttcaactct ttagaaacct
     2041 gctgctgggc tgccgtcatc tcagtccctg tcgtcgcctc cataggcgca ttatcagggc
     2101 gattcataac agccacagag accttggccg ctccctccga ttctttagga gcatcccccg
     2161 gtgccatagg aggcatcatg ccgcccttac tctggtgata ggtgtttacc ccattaaaca
     2221 tccattcata agccatatca gcgtggtcga ggtcggtaat ggggcagtaa acactggacg
     2281 catagatatc atcccgttca ttggcagcac caagggcctt taaatacggt tcataatcgg
     2341 aactgttccc cgtcgcaccc ataagggaag acaaggcacc gccggcactg gtcccattgg
     2401 agatgatttt ttccgtatca ccgggtaaac gaccggcatt atggcgtacg tagcggacgg
     2461 cagccttcat gtcgacgatg agggccggcg ctttaccggt ataggttcca tcagtacctt
     2521 gact
//
LOCUS       DAWDXZ010000246         2517 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8158_length_2517_cov_6.629569, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000246 DAWDXZ010000000
VERSION     DAWDXZ010000246.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8158_length_2517_cov_6.629569"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          565 a          571 c          671 g          710 t
ORIGIN      
        1 ggtattcttt gctccggccg tcgccattcc ggccgccgtc ggcgctcttg ttcatcaggt
       61 aacgggatct atcttggcag gcctctttgc tcgccacatg gatgccagag aaaagaaacg
      121 taatgcggcc ctccaagcgc aggccgttgc cgttccgggc aatcattaat catctgttgt
      181 cagtattctt tattccgtat ataatggact cataatgaat atcggctcgt tccttacggc
      241 aaatagccgg catggcttcg ccgttggacc tgtgtttaga aaaggagatg ctgtgttatg
      301 ggtggccgtt atgaagtacg ctttcgttat aaaactacga gcaaaacgtc tcgaggcagt
      361 gtcagttcca ccatggtaaa tgcttccagt gcttccgatg cacgaaatca ggttattgca
      421 tcgcacagca gtgggaatgg gatcacggtt atctccgtgg tgaaaaagcg gtaatcgaat
      481 aaaggttgat agaatctaaa cttgagaggc tcctctttaa cgtcgggacc ggaataaaat
      541 ctggtgcggc ttaaggggag cctttttaca actgaaatat ataagattta tgtatacgaa
      601 aagtataact taaaataatt agataaaaag atagataatc tattgactat taatttagac
      661 ggatgtataa ttatcaccaa caaatacaaa acagtgttga atggaagtag taatgttagg
      721 gatttcatga cagagacctg ctataggctg agaggcagga tggaatcccg gcgtgaagtc
      781 atccaggagc tgctgcccga acgcagattt gccagtagac gcagccggca ggcaaccgtt
      841 atccggccag gcattggaag ggaatatccg gagatgctgg aaaagtggtc gttatgacaa
      901 taagagtggt accgcagagt gaaggctttg tctctttttt agaggcaaag tctttttttt
      961 gctccatcaa aaccgtttgc gtatttgtat cttatttagt gtaatgtgaa aggatgtgcc
     1021 ttactatgat ggatttcatc ggaaagttaa gcggttttgt tggaaagaat ttgacctata
     1081 tcgttctcgt cgtcgttgtg gcagcttact ttatgcccag tgccttttta tgggccgtgc
     1141 cgcatacggt cctcctcttg ggggtcatca tgttcggcat gggcatgacc cttcatgcca
     1201 gcgactttaa attgatcctc cagcggccga aggaagttct cgtcggttgt accgctcatt
     1261 atacgatcat gcccctcttg gcgtatggcc tggtcctggc ctttgaaatg ccgccggaaa
     1321 tggctgtcgg tatgatcctc ctcggttcct gccccagcgg tacggcctcg aatgtcatgg
     1381 gcttcttggc gaaagccgac gttcccttgt ccgtttccat tacgacctgc tcgaccctct
     1441 tggcgccgat catgatgccc tttatcgttt ggggtcttgc cggtcagtgg gttgaagtct
     1501 cctttgtggc catggccatg acggtcatga aagtcatctt ggtcccctta gtcctcggcc
     1561 ttttggctca tcgcctgatg ggggaaaagc acgtcgtttc tttgtcgaaa atcttggttc
     1621 ttgtttccgc ttttggtgtt ttggttatcc tcggcggtgt cattgctgtc aatggcgcta
     1681 agattttgga aatgggactc tttgttatcg tcatggttct ccttcacaat ctcggcggtt
     1741 tccttctcgg gtatttcgtc accggaaaat tgggtttcgg gaaaaaacag cagcattcag
     1801 ttacccttga agtcgggatg caaaacgatg ccctggccat ttccttagta tcggtattct
     1861 ttgccccggc tgtcgccatt ccggctgctg tcggagccgc catccatcaa gtaacgggat
     1921 ctatcttggc gggaatcttt gcccgtcata tggaagctcg ggaacagaaa caacgtgagg
     1981 ccatcaaggc gcaggccgtc atggatgccg taccgggaaa tcattaggtg gcagggtcat
     2041 cgttgtacga aaaaaaagca ccattctcta tgagggaatg gtgctttgtg ctgccaatca
     2101 gaaattttca aaagatatga actttttaag ccccagaggg catgcttaaa tcgactgcgg
     2161 gaaggtgttg gtcagaattt tcgcacctcc gtcagtcgtt ttggtttgca gtcccggaat
     2221 ggtattgccc gtcgtttcga ggtccgtcgg aatgcgttta tacgggagct ttcgtaagat
     2281 ccgggtcatg gccggataga ggacgtcgtc gggattgttt agaatcgacg tcgattcgat
     2341 gtcgtagccg ctgtcggaat agctgctcat gcggtcggtg cgcttgtcgt aggcatagac
     2401 tgtcagacgg tagcggtatt cggtatacgt ttcgccgccg tcgcggaagc gatagcttcc
     2461 catgaactga cgccagtacc aggtctgtac gaccgggacc aggacgacat cggcgtt
//
LOCUS       DAWDXZ010000247         2516 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8159_length_2516_cov_13.616822, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000247 DAWDXZ010000000
VERSION     DAWDXZ010000247.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8159_length_2516_cov_13.616822"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          532 a          725 c          635 g          624 t
ORIGIN      
        1 tacctgctgc caaggtgacc acccctgccg aagccgccgg ctcggataac tttttggccg
       61 gcgtcaaata agccgaatga ccttcgatgc gagtaatgaa gcattcatag gtcgtaccgg
      121 tcacgtcggt gacgggaatg gtgtcgccta cggtgtggcg caggacgagg acgacgtgac
      181 gggcgtcgtc ggccgataat tcaaaggtcc cacaaatggg aaaatccgtg aaaatcttac
      241 gcatgatctc acctcgtcaa tgtcacggca taccagccgc ttcggagctg ttcatcagcc
      301 caattaaatc cggcttcttc agccgcctgc cttacgtcat cgatgcggtc gtcgatgata
      361 ccgctgacga tgagcgtacc gccgtctttt aagtatggcc ctactgccgg taagagagcc
      421 aatacggcgt tggtcaccag attggccacg ataacatcag cctgttgccc cttgggaata
      481 gccgtcaaaa ggtcgctgtt ggcgatggcc atccggtctt caacgccgtt gagggccgca
      541 ttgacccgag cctgagcgac ggcggcgctg tcaaaatcga cagctgctac gtgagcggcg
      601 cccagctttg ccgaagcaat ggctaagatt cccgtgcccg tgccgatgtc aaaaacgaca
      661 tcgcccggcc gcactgcctc ttccaggtat tggacacaga gacaggtcgt gtcgtgaaag
      721 ccgctgccaa aagcaaggcc cggatcgatg gtaaggacgt cttctcccgg ttccggcgat
      781 gctttttccc aagccggttc cgcccagaaa tggcgggaaa tcttcttggg atggaaatga
      841 tcctgccagg caaagagcca atcttcgtcg ttgacatttt tttcaatgac ctgccagttg
      901 ccgaggtcgg cgccgcggct ttcaagggca ttcatttttt ctcgaatcgt ttccaccaag
      961 ccttcttgtg tcagcgggaa atacgctgta atgcgaatgc ggccctcagc atctggctgc
     1021 tcatcgtcga tgaccgagcc gttgctgccg cattcataaa caagaagggc caccaaatcg
     1081 gtggccattg gggaaacgag caaatcgact tcattccatt gcatcgtcta tgctcccttc
     1141 atctttacag atcttctttt aatcgggccc aactccaaca gaagtcgccc catactttcc
     1201 gcaagctctg aaacaaatcg tctttcaact tgttccagac gcttttcttg ctctttttgg
     1261 tatctttttt cgtattgctc acagcactgt catcagcctt tttttcagcc gactttggct
     1321 cccccaccca cgtttcaccg ctggcatagg cgaattcacg caggagttcc tgctgtttat
     1381 cattcaattt cttaggaatg gaaacggtga cccggacgtg gtgatcgccg cggcgattct
     1441 tggcaccgag gaccggaatg cccttgccgc gaatgcggaa caaggtaccg gtttgtgtac
     1501 cggccggaat gttcaactca atctttccat ccaaggtttc gacaggaatc ttggtcccta
     1561 aggctgcctg ggcaaaggaa atcgtttctt ctgacagaat atcgcttcct tcacgggcaa
     1621 acttcggatc gcggcgaacg aagatgtaga cgtacaaatc gccgtgaccg ccaccgcggg
     1681 tacccggttc gccttcaccg gcaacgcgca ggcgtgcacc gtcatcgaca ccggcaggaa
     1741 tcttgatttt cagtttcttg cgaactttta cggttcccga gccatgacat ttcttgcatt
     1801 tgtcatggat aatcttgccc gaaccgccgc agcggctgca agtacggaca ttgaccatct
     1861 ggccaaaagg cgtgttctgg acgacacgct gctggcctgt accgtgacag tcagggcagg
     1921 tatcgacctt gctgcccggt tcggcacctt cgccgtgaca gtggtcacac gtttcttcct
     1981 tgggaacttc aatttccatt tccgtcccaa aagcagcctg cttcaaggcg atgtctacgt
     2041 catagcgcag atcggcgcct cgctgaggcc cgttggacga ctgccggccg ccttgaccgc
     2101 caaagaaaga atcaaagata tcttcaaagc cgccgaagcc gccctgacca ccctggccga
     2161 aaccggcacc ggaaccggca gcattcccat tctggaatgc tgccggaccg aactggtcgt
     2221 actgggcgcg tttttgcgaa ttagacaaga cgctgtaggc ttcgttgatt tctttgaact
     2281 tttcttcagc tgccttcgga tcatcagggt tgcggtccgg atgatactta agggccagct
     2341 ttttaaaagc tttttttatt tccgcatcgc tggcgtcttt ggaaacgccg agcacttcat
     2401 aataatctct tttgctcatc tgagaagccc ctcttatcta tcttacttct tatcgtcgtc
     2461 gactacggtg taatcggcgt ctactacatt gtcatcctgc ttctggctgt caccgg
//
LOCUS       DAWDXZ010000248         2512 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8177_length_2512_cov_10.273504, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000248 DAWDXZ010000000
VERSION     DAWDXZ010000248.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8177_length_2512_cov_10.273504"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1740..1749
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          480 a          783 c          608 g          631 t
ORIGIN      
        1 tcttcctttt tttcagtaaa ataatactat attaaactat tcgtggaaaa gaggtcatta
       61 tcaatgcata tccctgagaa ttatctgagc ccttcgacgt gcggcgtcct gggggcggtc
      121 atggttccga tctggctgct gtcgatccgc aaggtcaagg aaacggtccc tcgtgaaaag
      181 ctgcccctcc tcggcgtcgc tgcggctttt tccttcttgg ccatgatgtt caacgttccc
      241 cttccgggcg gtacgacggg tcatgccgtc ggcggcacgc tcatcgccat cctcctcggc
      301 ccctacgcgg cctgtctctc tgtcagtgtg gcccttctca tccaagccct cttctttggc
      361 gacggcggca tcttagcctt tggcgccaac agtttcaaca tggcctttgc cttgcccttt
      421 acgggatatt tcgtctatca cggcgtaaaa aaactcatct cttcggatat catcgctgcc
      481 ggtatcggct cttatgtcgg catcaacgtc gccgccttct gcgctgccgt tgaattcggc
      541 atccagccat tgatcttcac cgatgccgcc ggtcaggcat tatactgccc gtaccctctg
      601 tccatttcca ttccggccat gatgatcggc catatcacga ttttcggttt ggctgaagtc
      661 atcttcacga caggtgtcct ggcctacctc aaaaagacat cgcccgcttt atttcatatg
      721 tcggctccgg cctcgtcgag taaatccttg ttcggactct tggctgccct cattttggcc
      781 acgcctttag ggctcttagc tgccggtacg gcttggggcg aatgggatgc ggaagaactg
      841 gcagaaacgg atttcttcgg ctcggcctta ggctatacgc cggccggtat ggaaaacggc
      901 ttctccttcg atgtcctcat gccggactat gccattgcag ggctccccga cgttttaggc
      961 tatatcattt ccgccctcgt cggcacggcc ctgttgatca tcatctttaa agtcatcgcg
     1021 tccttcctgc cggccaagcc gtcctttgac acaccatctc agcattagga ggcctaaacg
     1081 atgaaactcc ccgaatgggc gcagcgtgaa gaaatttata atcccggccg tgaccgggat
     1141 tatttcattt cccgctctct cctgcgctta gtttctatat tgaaggccct gcgccaacag
     1201 cacggcccgt cgcgatggtc cgtcggtgcc ggtccggcct tgctgacgac cttactcatg
     1261 attctcctca tcgtcctctg tcggaccatg gcctttctct gggctgtcct ggccggtgaa
     1321 ttggtcctcc tggccttcca tccgggacgt gagctgcgca cgaccttggc ggcagccctt
     1381 gccgcttccg ccgtctgtgc cgtcatcgtc accccggcct tttttctcgg caacggacgc
     1441 cacgtctttc tcctgccctg taaaacctgt ctcaccgtcc tctgtctgat gctccttcag
     1501 caaaacgtgt cgtggcacaa actgacggcc gccctcaagg cctttcacgt tccgtcgctg
     1561 gtcattttca tcttggatac gaccttacgc tacatcgcca tcctcggcca agaatcggca
     1621 gaactcttga cggccctgaa actgcgctcc gtcggccata atcccgataa aaaagcggcc
     1681 ctcagcggcg tcgccggcat gatgctgcaa aagtcgcagc gcctctctca ggacctctan
     1741 nnnnnnnnng agggaaaaag gcgttccttg tcaccggcta cgggcgtgct gctcctgatg
     1801 ctcttatctt acgtctatct cttcatccgt ttggaaggag ttctttcatg attattctcg
     1861 acgacgtctg ttttgcctat gacgacagac ccattctcag tcacctgacg gagacgatcg
     1921 aacccggtca ggccgtcctc ctcaccgggc ccaacggctc cggcaaatcg actctcctgc
     1981 ggctgttgaa cggccttata ttcccgcagc tggggaccta tacctttgac ggcacggtca
     2041 ttacggcggc caagatgcgc gaccaccgct attcaaaatg gtttcaccag cgcatcggct
     2101 atatctggca gcatcctgat tcgcagctct tttgcagcag cgtccgcgaa gaattggcct
     2161 tcggtcccgt ccaaatgggc ctcccttccg aagaagtcaa aaaacgcgtc gatgatgcta
     2221 tggatctgtt gggcctgacg aaactggccg acagggcgcc ttattccctg tccggcggcg
     2281 aaaagaagcg gacggctatg gcctccatcc tgaccatgaa tccccagatt tggaccatgg
     2341 acgagccgga aagctacctc gatgccgacg gccttgcctg gttggaagat ttcctgccca
     2401 gcctgaaagc agccggaaag accctcatcg tagcgaccca ccacgccggt atcatcgaaa
     2461 agattatcga taaagaaata tcaatgaagt cataaaaaaa cagatgcaga cc
//
LOCUS       DAWDXZ010000249         2512 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8178_length_2512_cov_6.636142, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000249 DAWDXZ010000000
VERSION     DAWDXZ010000249.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8178_length_2512_cov_6.636142"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1265..1301
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          713 a          570 c          613 g          579 t
ORIGIN      
        1 ggaagtatta taaggagggg gtgttctcaa tgggtaaaat gcacgttaaa acaggcgata
       61 cggtcattgt cattgccggt aaagaaaaag gcaaaaaagg caaggtcatc gcaacgtatc
      121 cgaaaaaaga ccgcgtcgta gttgaaggcc ttaaccaggt taagcgccac acgaaaccga
      181 gtcagaacaa tcctcaaggc ggcattatca ccaaggaagc tgctatccat gtttccaacg
      241 taatgctcgt agatcctgaa agcggcaaac cgactcgtgt caaaatggtc cagcaggccg
      301 acggcactaa agttcgtaca gccgtaaaga gcggtaaagc tatctaataa gaagggaggc
      361 ccttgagagt gtccagatta aaagacaaat acctttccga agttgtgaaa ggtttacagg
      421 aaaaatacag ttataaaaat gtaatgcaga ttccgaaggt taccaaagtt gttatcaaca
      481 tggccgttgg cgaagctgtt accaactcca aagccttgga tgctgctgtt agcgatatga
      541 ccatcatttc cggtcagaaa ccgatcatca cgaaagctaa gaaatctatc gcagctttca
      601 aaatccgtga aggcatgaac ctcggctgca aagttacgct tcgcggcgaa cgcatgtatg
      661 agttcctcga taaattgatg aacttgacgc ttccccgtgt ccgtgacttc catggtgtca
      721 gtgcaaccgg cttcgacggc cgcggcaact acaccctcgg cctgaaggaa cagcttatct
      781 tcccggaaat cgaatatgat aaggttgata aagctcgcgg catggatatc accatcgtta
      841 cgacggctgc gaccgacgaa gaaggtcgcg acatgttgaa actgatgggc atgccttttg
      901 aaaagtaata gaggaggata acatacatgg caaagaaatc gaatggttgc caaatacgct
      961 aagcttcgtg ctgaactcaa agcaaaaggc gactatgaag cactttctca gctgccggct
     1021 aacgcatctc cggttcgtct gcacaaccgc tgcaaaataa ccggccgtcc tcacggttat
     1081 atgcgtaaat ttggcatctg ccgcatcaca ttccgtgaac tggcttataa aggtcagatc
     1141 cctggcgtta aaaaagccag ctggtaacac gtattccatg gagggaggtt tttagattat
     1201 ggcaatgacc gatccgattg cggatatgct cacaagaatc cgtaatgcta actcggcata
     1261 tcacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naactcccgc gcgttctcgg
     1321 tggtttgggg atcgctgtaa tttctacgtc tcaaggtgtt atgagcgaca aacaggctcg
     1381 taaactcggc ttgggcggcg aagttctcgc ttacgtctgg taaattttgg aggtgtcaat
     1441 atgtcccgag taggtagaat gcctattgat attccggcag gcgtaaatgt aaacctcgat
     1501 ggccacgtaa ttaccgtaaa aggcccgaaa ggcgaactca cacgtacgct tcaccaggat
     1561 atgaaagtca caattaatga caacgttatc actgtagaac gtccgtctga cgataaaaat
     1621 caccgtgcac tgcacggcct cacccgcgca ctcgtagcca acatggttaa aggtgtttcg
     1681 gaaggcttca aaaaggaatt ggaaatcgtc ggtgttggtt acagagcaca gatgaaaggt
     1741 aagaaacttg ccttgactct tggtttctcc catcccctcg aactcgacgc cccggccggt
     1801 gttaccatcg aatgcccgag tgctacgcag atccacattt ccggtgcaga caacgaagtt
     1861 gtcggtgaat tcgctgctaa agttcgcggc taccgtctcc ctgaaccgta taagggcaaa
     1921 ggtatccgtt atgttggcga acatgtccgt cgtaaagccg gtaaagctgg tactaagaaa
     1981 taagatttaa ttagaaagga gtgaattcct tgaataagag taaaaacaca atccgtttga
     2041 aacggcattg gcgtttacgc aagaacatca acggcacggc agaacgtccg cgtctcaacg
     2101 ttttccgcag ccttaacaat atttatgccc aggtcatcga cgacgtaaac ggcgtaacct
     2161 tggttgcagc aagctctttg gacaaagaac tgaaagatca gaacgtcacc ggcggcaacg
     2221 ctgaaggcgc aaaattggta ggtgctttgg ttgctaaacg agctgttgaa aaaggtatta
     2281 cgactgtcgt tttcgaccgt ggcggttaca tctatcacgg ccgtgttgca gctctcgctg
     2341 aagcagctcg tgaagctggc ttgaaattct aatcaaggag gaaataacac atggcaaaag
     2401 ttgaccatgc aggtctcgaa ttacaggaaa atgtagtatt catcaaccgt gttgctaagg
     2461 tagttaaagg cggccgtcgt ttttccttca gtgccctcgt tgttgtcggt ga
//
LOCUS       DAWDXZ010000250         2509 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8189_length_2509_cov_9.782396, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000250 DAWDXZ010000000
VERSION     DAWDXZ010000250.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8189_length_2509_cov_9.782396"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          733 a          629 c          578 g          569 t
ORIGIN      
        1 cttcttcggc gatgagaagc cccatcgctt caccaatagt aagcacattc ttcatgataa
       61 actcctttta aaaagataga gccgcttgaa acagcggctc atcaacaatt atggtttaat
      121 tattttaacg gtaacggttc aactcgacca acgaggaaca agtaagacaa ggcaccgagt
      181 atgcacaaga caccagccgc tgcaagggga accaggaaag agccttcggt aatcgtcagc
      241 atgacaccgg taaaggtcgt aatgaaaata cctgctaagt tgccggcaaa gttcataatc
      301 ccgccgatag aagccacatg cttaggcgta ggcgcaattt caccgacaat aacccacgta
      361 ttagagccag caaaggataa gcttgcataa gacaaggcaa aaagggccaa gcagatatag
      421 atattatcga cgaatgcaca aagggcaatg caggaagaaa gaagcatgcc gccaactaaa
      481 caaagtttac gtgaaaacgt agccgaatgg ccgcgacgca agagatagtc agaggtaagg
      541 ccgccaagcc atccgccggg aatcgccaac agtgccggca gcatccccaa ggtccccaat
      601 gctttcaaag aaaagccgcg ggtttccatt aagtagctgg ggaaccaagt gataaaaaag
      661 tagatggcaa aattcaggca gaacaggccg atcataaggc cccaaacggt cttatagcgg
      721 aacaaatcag cccaagaaac cttgccttca ccggcttctt tagattccga agcctgattt
      781 tcaccgcgtt cagctaataa agcttctact tgttccggtg caatatcgcg atatttttcc
      841 ggatcacgat aaatgaacca ccaggcgagg gcccaaccaa taccgataag gcctgtaata
      901 ataaaggagc ctcgccagcc cattacagaa atgatccaag caatcaacgg cagcgataag
      961 gccgaaccga cacgggaccc gctgtcaaag atactgcaag caatgccgcg ttcttttcgg
     1021 ggaaaccagt tatatgcagc ttttgcaaag gaaggcatcg ccgccgattc accgacacct
     1081 aacaagagtc gggacccgac catatgaccg aaagtatgaa ccataccggt cacggccgta
     1141 aagacagacc accaggcaac agcaaaggcc aagctcagac gaacaccgac tttatcgata
     1201 aaccagcctg ccggcatctg gcacagggca taggtccaaa agaaagcact catgatgacg
     1261 cccatcgtag ccggatccaa ggccagttca gctcggatat gaggagctgc aattgccaag
     1321 ttggcacggt caatatagtt aatggcattt gccaggaagc agagaccgat aataatccat
     1381 ctcatatttt taaagtttcc ttttgtcatg ataattctcc tttacaactg tccgcatgta
     1441 cggttaatcg catcccacaa gccacagaga tagttagccc caagtgcacg gtcatataaa
     1501 ccatagcccg gacgaccttc ttctccccaa atcatacggc cgtggtcagg acggacgggg
     1561 ccttcaaaac cgatatcgta agcagctttc ataatttcat acatatctaa gttcccgatc
     1621 tgggatttat gggctacttc attgaacacc cacggttcat ggacctggat gttgcggaca
     1681 tgaaggaagt ttatgcggcc cattttaccg aatttacgaa tgatgtgagg aatatcgttt
     1741 tgacggttcg agccgagaga gcccgtgcaa atcgtaaagc cgttgctttc gctgtcgacc
     1801 atcttggcaa tcttcgtgag gtcactttcg tttttgacga tgcggggcag tccaaaaaca
     1861 gaaatggggg gatcgtcggg atgaatcgcc attttgatgc ccacttcttc agcaacggga
     1921 acaatggctt tcaggaaata ttcgagattt ttgcgcagac cgtcttcatc catgccctgg
     1981 tactgttcaa tcaggtggga cagttctttc aaccgttccg gttcccatcc gggaaggaca
     2041 aaaccattcg tattatcaag gatttcccga gtcatgtctt ccggcgtctt accggcaatg
     2101 agatggtagt cataagcgag aaccgtcgag ccgtcataca agggtttagc aagttccgtc
     2161 cgagtccagt caaagacagg catgaaattg tagcatacca tttcgatacc ggccttggca
     2221 gcattgcgaa gggacgtaat ataattttcg atgtactggt cgcggctcgg caggcctaat
     2281 ttaatgtctt catggacatt gatgctttcc aaatctttga atactaagtt gttggcttcc
     2341 gcctgctttt tgatatgcat caacggttca accggccaca cttcaccgac aggaatatca
     2401 aataaggcac cgacgatgcc atccatgccc ggaatttggt cgatctgctg cagtgtaatt
     2461 ttgtcatctt tctctccgta ccaacggaat gtcattctca tagattaat
//
LOCUS       DAWDXZ010000251         2508 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8197_length_2508_cov_5.119853, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000251 DAWDXZ010000000
VERSION     DAWDXZ010000251.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8197_length_2508_cov_5.119853"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1313..1322
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2166..2175
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          778 a          548 c          567 g          595 t
ORIGIN      
        1 catgatggaa atttcgccac cttgaaggat gacgaacgat ttctttccat gaccgtaccc
       61 tgccaagaaa aggccgacta ctatggcttg gtcacgcgca gctatattga agccgatgcc
      121 aaaacgggtc aggccggcta taccgattac cgctttgtcc gcatcgaatc agccgaaggg
      181 gggagataaa tatggcatcc attggagata tcatgaaggc aaaaaaagaa gatagggatc
      241 ctaaaaagca agaacaactg agtcctaaga agcagaaaaa agtctctatc caattagata
      301 caagcttgga taaaaaaact cacaaatccc atcacaaagg caaagatgat ttcaacaagg
      361 gaagcaagtt gtcgaacagt aatccgccca aaaaggtaaa taaaagtaag gaagccactg
      421 cggcctataa ttttattccc tttacacgaa ctgttttggc atcgcctctc gatgctatga
      481 gaactgactt tcgtggctat atccacagtc aagaaacata cagcggcact ttggcattgg
      541 aactttccaa tgagacatca ctctttattg gtggtgatca aaaaaatgat gaagagcttt
      601 ttttcgcgcc tgatggaaaa ccgcggattc cgggaagtac cctgcgcggc atggtgcgca
      661 atctctttaa aatcatcacg gccggcacca tgcgtcgtga cgaagacttt accgaccgcc
      721 atctctattt tcgttgtatt atggcgccta atgggatgcc gcaattaaaa gaattgaatg
      781 cctattatga acaacgaatg gtatctgatg aaatcaatcc agagacgaac agacctaaaa
      841 aaaatgcaag accggctttt ctttaccgtc tcgaaaggga aggtgcctat tatatggcac
      901 cttgtgatat gaaatttgtc ccgataaatg aactaggcta tataagaaag agtgaagttc
      961 ggtggagtat acccgaaaaa aaagcctaca ttgtgactgg gaagcaaaaa gataagaaaa
     1021 atgtacgcta tttatttgcc ccgcgttggg aagatgctga gttcgttcct gataaaatcc
     1081 tacaagacta ccgagacgat acgcggcgca aaggggtcaa cctcttggaa gataggggaa
     1141 gttccaaaca acaaactcca ctacgaggca agtccgcaaa agattttgct gaacgcgacg
     1201 atatcgactt tattgtgcct tgcttttata ccaaaaatga agacggagaa attacctcct
     1261 tcggccatgg ccgttcttac cggattcctt acgagcaatc tatcggcgat cgnnnnnnnn
     1321 nntcgtcgat tttgctgacg ccgtttttgg tcgcaaggaa gactgggccg gccgcgtatc
     1381 ctttgaagat gcttgcttaa agggaccagc gaatttagaa tctgctggtt gggtaaaacc
     1441 tttattgggt gccaacccga cgtcgtatca actctatttg actcaaaatg actggccgcc
     1501 gacccattgg gacagcgacg cttccgtaga gttgcgaggc tataagatgt attggcataa
     1561 aaacattggt ggaaatgatt ggaaggcaaa acccgaacaa caaaatgcta atattacgaa
     1621 aaagatacag cctttatcgc ccggtaattc tttttccagc gctatccact tccgaaacct
     1681 gacggctgtt gagttgggag ccttgttaaa agtattccac ctccaacagg atgataaaga
     1741 agatatcgtc tataaactcg gtatggggaa atccttaggc atgggcagtg tgcgcattaa
     1801 atcgtccctg cacctcgaca gtgaccggta cgggaatctc tttgacggcg aaaaatggca
     1861 ggagtcacgg gaatcagcag atgcacagcc atttatcgaa gcctttgata cttatgtcaa
     1921 agataccctt acccctgatg aagcattatc ctaccagtcg tccttaaaga accttcgcat
     1981 catgctggat tggcaatata taaaacggcc aggatggatt gagaaagtca gtgcagccgg
     2041 tagcgatagg aaaaatgtaa gtggtgatat ccttaaacaa tttacggacc gagcagtctt
     2101 gccgacaccg gataaaatca tcaaataaaa tagaagagaa ggttgccttc acaggcagcc
     2161 tctctnnnnn nnnnntggag tctgatgggg aaattttacc aaaactatat aagaatggta
     2221 tcggaccttg ctatacgacc aacgaatcgg ctgtccgcta ctgtgtacaa aaacacggat
     2281 cgatgaagcg gctgttcgtc tttgccactc agacggttca attgccttta acgtataaga
     2341 ctgaagaaga cataataaaa gagtatcacg atgaagaaga ccatacctat acccatctct
     2401 cttatttcga gcatcgcctg caacaagggg agcaccccat tgagactgaa ctcgaagttg
     2461 ctgactacga cgaaaatgct gacatgacgg agaatatcca atctatcg
//
LOCUS       DAWDXZ010000252         2508 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8195_length_2508_cov_8.604158, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000252 DAWDXZ010000000
VERSION     DAWDXZ010000252.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8195_length_2508_cov_8.604158"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2233..2242
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          556 a          623 c          652 g          667 t
ORIGIN      
        1 taacttgcac aactcggtcg atggggatat acttttaatt atttagaaca aaatattgta
       61 agaaagaagg agaatagcgg tttgaaacga aaaattggtt tgagtacaca gattttgatt
      121 gggttggcca ttggggcctt ggtcggatat ttgtttcctg attttggaga caagctgaag
      181 cctgttggcg acgctttttt acggatgatt aagatgatcg tcgtccccct tattttcagt
      241 accctcgtca tggggattgc cggcacgggc gattttaaga agctcggacg ccttgggggc
      301 aaggccatca tttggtttga gtttgcgacg accattgccc tggccatcgg cctgttggtc
      361 atgaatttag cagagcccgg tgtgggcgtt accttgtcgg cttcggcagc taatgcgtct
      421 catgcggcag cggccagtca gcacagcgtc gacctcgtcg actatgccgt tcatatcgtg
      481 cccagtaata tcatcgatgc catggctcgt caggatatgc tgcaaatcat tttcttcgcc
      541 tgcttctttg gcgtcgccgt cgctcatatc ggtgaaaagg gtgagctgat cgtcgatttt
      601 tgtcagagcg tggccgaagc tatgttcaag gtaacggcct atgttatgca ctttgcgccc
      661 ctcggcgtct ttgccatgat tgcctacact gtcgggagtt acggcattgc catgctcatt
      721 cctttgggcg aactcatcgt ggccgttgcc ggtgcgacga ccttatttat ctgtattgtg
      781 gccctcatcg ccagtgcttt tacgcgaatc aattttttct gcgtcatcca cgctttgaaa
      841 gacatcctct tcttagcctt ttcgacggcc agcagtgaag ccgcccttcc cggtgctatg
      901 aagcgtttgg aagccctggg tgttcctaag ccgattgtta cctttgtcat gccgacgggc
      961 tattccttca acctcgacgg ctcgacgctg tacagttcgg cgggtatctt gtttatcgcc
     1021 cagctctacg gcattcagat gcccattgaa cagcagctct tggtcatggt caccctcatg
     1081 ctgtcgacga aggggattgc cggcgttccc ggtgccggta tcatcgttgt cgctgccacg
     1141 gctgcggcct tcaaccttcc gacggaaggc gtcgccattt tgctcggcat cgaccgtatc
     1201 ttggacatga tccgtacgat ctgcaacgtc tgcggcaact gcatggcgac ggtcgttgta
     1261 gctttctggg aaaaggattt gaccaaggct gatttctata aaaattatcg tgcgtttcta
     1321 gaggacaaag aagcctaaac ttgccgagag tttcaatagc aatactagac taaatctgtt
     1381 ataataatgg ggtaaacaag tgatccgtaa ggcatgtctg acggaaaact tcatcgatga
     1441 gcctccgtgc tcttctgcaa aggactgtct gcgcaggcga ggcgaaagca catagtgatt
     1501 atcctaaggt tctgtgcttt cacacagaac cttttttgtt tattaaggag gagaccttat
     1561 ggaaacgcga gtcgctttaa tcggtattat tgtcggcaac cgcaatgaat caacgaaatt
     1621 gaatgaaatt ttgcatgaat acggtcagta cgtcatcggc cgcatgggcg tcccctacaa
     1681 ggataaagac atcaacgtta tcagcatcat cgtcgatgcg ccccaagacg tcatcagcgc
     1741 catgtcgggc aagatcggca tgattccgga agtcagcacc aagacgattt atccgcctct
     1801 gccgcagtag gagggacgta tgctgaaaat tgcaatttac ggtaagggcg gtatcggcaa
     1861 gtcgaccatg accagtaact tggctgctgc ctttgcctat ttggggaagc gggtcattca
     1921 gatcggctgt gaccccaagg ccgattcgac gttcaatctc ctcggcggcg aacccttgct
     1981 gccggtcatg aattatctca gggagcacga cgaagatccc gagtcgattg aagacatttc
     2041 ccgcatcggc tacggcggca tcctctgtat tgaaacgggc ggaccgacgc cgggcctggg
     2101 ctgtgccggt cggggcatca tcgcaacttt ttccctcctc gaagatttag gcctctttga
     2161 agagtataag cctgacgtcg tcttgtatga cgtcttgggc gacgtcgtct gcggcggttt
     2221 tgccgccccc atnnnnnnnn nnctacgcca aggtggaagg catcattttc aatcggcgca
     2281 atattcccga tgaagatgcc aaggttcgtg cctttgccga aaaggcgggt ctcaccatcg
     2341 tcggtgatat tccccgaagc gacgatatca accgttatga agaattgggt cagaccgtta
     2401 tcgaaggcaa tccggacctt cccatcagca agaccttcct cgatttggct cgaaaattat
     2461 tagaagacaa ggattgatta tgacagaaag aaaacctttt gccattac
//
LOCUS       DAWDXZ010000253         2489 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8256_length_2489_cov_5.403862, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000253 DAWDXZ010000000
VERSION     DAWDXZ010000253.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8256_length_2489_cov_5.403862"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2094..2217
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          607 a          605 c          485 g          668 t
ORIGIN      
        1 taaggcgatt attcatgctg acccccaaac cggcttctac catgcggtac gtcgtgtaat
       61 tatcaatcgc cgtataacga accttgcagg aaatattctc agctttcaag agcaaggccg
      121 aatccgtatc ctgttggggc agcgattcaa taaaggattc attcgctaac tccttcaagg
      181 gataggcttt agccttggcc aagggatgat tttccggcag ccacataacc atttcatctt
      241 cacacaaagg aatccaatct cccgaaaaag ggcgcttcgt cgtaatgcaa cagtcgaagc
      301 gcccttcttc gagcccttca tacatcgact tattggcaca ttcttcaata tggacgtgaa
      361 cggaaggata ggtctgttga aaggcctgaa tgatttgggg gagccagttc gcagcgacgc
      421 tgaaataaga accgatataa atgtcacccg ccgtcagacc gcgaagctgg gcactgtatt
      481 gaagtatcgt ctcttcatcg cgaatgagtt gccgcaagaa cggcaagaca gccgtccctt
      541 cacgtgtcag atagacgcct tgggccgttc tccctaaaag aggaaagcca atttcctttt
      601 caagggacgt aacggcacgc atgaccccgg acggagtata acccagccgt tccgctgccg
      661 cagaaaaact accggctcga acgacttgca agagaagtct gcatttttcc gtattcataa
      721 aaatcacctt tgctttttta tcaattcctc atatattatt ttgctattct ctccatgctg
      781 attatagcgt atcataaaag taagaaaaaa gtaaggaggt ttttgtatga aacagggaac
      841 tgctgaatta ttactcctca cagttatcgt ctcacgaagc atttcttatc tattatcaaa
      901 aatcggcctc catggggtac ctcccttaga attactggct atccgctttt tcctgactac
      961 catcatttta ctgattattt ttcataagcg gctgccaaat atcacaccgg cccttttcaa
     1021 ggccgccggc ttgttaggaa cgctgctctt cgcttgtatg gcctgtgaac tgatcagctt
     1081 aacgacaatt ccgtcttcaa cggttgcatt cttggaaaat acgtctgtcg tctgggtcct
     1141 cctgctcatg gccatcctca atcgccagtg gccgggaaaa caagtattgt tggcaacgac
     1201 actgctcttt atcggcatcg cctgtctcac gctccaaggg gcggccctca gtctggaacc
     1261 aggtgaaatc atctgcctga ccggggctct cttctatacc gtatggatct gcctgacagc
     1321 ccgcctggct cgagagcatg atcctttatt gctgggaatc ctacagatgg gatttctcgc
     1381 cgtttacagt accactgcca gcctgctgtt tgaagtgacc gtcatcccca ccgatacgac
     1441 aacgtggttg gcaatcatca ccttagtcct catctgctcc gtcttcggat ttaccttcca
     1501 gcccgtagct caaaaatata ccagtgctga aaaagccggc ttatttacgg ccttgaatcc
     1561 cttaatcgca tccgttctcg gtgcattttt cctcaatgaa atgtttacaa cgactcaatt
     1621 aatcggagcc atcttcatca tcacaggaat catcctggtt caattaccga ataaaattct
     1681 ctgtaaacta cattcctatc caaactaata cccttaagta aaaatcaacg aattatgaag
     1741 gagctatacc catgaaaaaa tatattgtcc tcttaatgct ggcgttagcc cttcccgttt
     1801 tgacgacgcc ccctgccttt gctgacccgg agactgccat acaggaatct tccgtgtttt
     1861 ctgatttata cggcctgcgc cgcactcagg aaatgacggt aatcagtccc gatgacgctg
     1921 tcagccattc cttttacggc ctaaaaatcg gcgataaaaa aagtgacgtc ataaagacgc
     1981 ttcgtccggc ctttaccctt atgtacgaat taaaagccat tgggcctgct tatttattga
     2041 gtgatgtcgt ctatcccgac tactatctga ccgtatcctt tgatgacaag gacnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaag
     2221 aagtaccaca ttatgactga tagtttaaat tcctaggcgc tactccgttt gatagccatc
     2281 tcgtcagatc attaaatttg tctcccattt tttatctcct tttattcgct ctgctaagta
     2341 attacagcag ggcatatata tggaagaagc actggcattc gtcagtgctt ctttttcgtt
     2401 agttaggggg aatttgtatg cattggtcgc ggcaatctaa aaaaggactt tataagcaaa
     2461 gagtgcttct ctctagttat ctggcagtg
//
LOCUS       DAWDXZ010000254         2489 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8255_length_2489_cov_8.487264, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000254 DAWDXZ010000000
VERSION     DAWDXZ010000254.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8255_length_2489_cov_8.487264"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    612..621
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          642 a          611 c          678 g          548 t
ORIGIN      
        1 cgtacgctcc caatgcctgt atgcccaggc cagctccctg gcacggaccg tattaaacct
       61 tccggtctgt atcacggcag tggccaatgt aaaatataaa caacccgtta ccgtaggcga
      121 taaactgatt gcaaaagctg acgtcatccg ccagcgcgac cagaaatact acgtatgggt
      181 tacgattcgg aagatgagaa aagaagtgtt ccgggcgaaa tttattatag aatctttgga
      241 ataatggagc ggtagtatca tgaaaatagc agtagatgcg atgggcggcg attatgcacc
      301 ggaagaaatt gtcctcggtg ccatcgaagc cgtcaaagca tatgattacg atgtcgtcct
      361 ggtcggtgat gaaacgaaga tacgccaggt attgacgacg catggggcaa aagaaaatga
      421 acgcctgtcg atcgtccatg ccagtgaagt catcgaaatg ggcgagcatc cggctcaggc
      481 cattcgccgc aagaaagacg catccatcgt cgttgctgcc cgcttggtaa aggacggcgc
      541 atgtgatgct gtcgttgcag ccggcagcac cggggctgcg gtaacggctg cccttttcga
      601 actgggccgc annnnnnnnn nattgaaaga ccctgcatcg ctacgccgat tccttcgaaa
      661 aaggggatta ctgtcctcct cgattccggg gccaatgcca gcagcaagcc gaagcatttg
      721 gtcgaaggcg ccattatggg ctgccattac gccaaatata tcctcggcat cgaaaagccg
      781 gctgtaggcc tcttgaacat cggtgaagaa gcgtcgaagg ggaatgagtt gtgtcaaaag
      841 acacatccca tgttggaaaa tttgaaaacc attactttct atggtaacgt agaaggcaga
      901 gatataccga aaggaacggt cgacgtcgtc gtctgcgacg gattcgtcgg caatgtcatc
      961 ctgaaatttg gggaaggcat ggccatgttc atcatccgtc tcgtccgcga cacgatcaaa
     1021 cagagcggct tcatcgctaa attaggggcc ctggccatct atccggcctt gaaatcgctc
     1081 aagaagagac tggactttac cgaatacggc ggagcaccct tattgggtgt caacggcagc
     1141 ttcattatct gccacggcag ctctaaagcg aaggccatca aaaatgccgt ccgcgtggcc
     1201 ggcgaaattg cagagcaaga cgtcatcggc catattcgaa aaagtataga ggaagaagag
     1261 gtagaacagt atgatagtga aagctaacgc agccggcgtg ctggggacgg gccattatgt
     1321 acccgataga gtcgttacca ataccgaatt ggaaaagtat gtcgatacat cggatgaatg
     1381 gattcagagc cgtacgggta tcaaaacgcg gcactttgcc gaagacggaa tgaatacgtc
     1441 ggacatgtgc accatcgcgg ccaaacaggc cctggaaatg gccaatctgt ctgctgaaga
     1501 cctggatatg attatcgtct gcaccttgac gccggacatg acgattccgt cgacagcctg
     1561 cattgtacag gataatatcg gtgccaagaa tgcagcggtc tttgacttgt acgctgcctg
     1621 ctcgggcttt gcctatggcg ccattacggc gtcacagtac atcgaaacgg gcatgtaccg
     1681 ccacgtcttg gtcatcgggg ctgaaatcct cagccgtttc ctcgatatga ccgaccgtaa
     1741 cacgtgcgtc cttttcggcg atggagccgg ggccgctgta ttcggtcccg tcccgcaggg
     1801 ctacggcatc ctcggtgccg acatgggtgc tgacggaagc ggcggtaaat acctgagcat
     1861 cccgtccagc ggcgtcgcta ttttgccgac ggacgaagcg cgggcaaaac acttgaccta
     1921 cgttaagatg gacggtaagg aagtctataa atttgccgtt aaagttatgg gacggacggc
     1981 ggaaaaagcc ttggaacgag ccggtctcag ttatgaagac atcgacctca tggttcctca
     2041 tcaggccaat acccgcatca tccaatcggc agccaaacgg ctccaccttc ccatggaaaa
     2101 agtctttgta aacattgata aatacgccaa cacgtcggga gcatccattc ccattgccct
     2161 cgatgaagcc ttacgtcaag gccgtatgaa gcgcgatgat atcgtcgttt tggccggatt
     2221 tggtgccgga ttgacgtggg ccggcttagt tatgaagtgg tattgatttt ttgcgagagg
     2281 tgtgattgat gatgaagaaa gcctttgttt ttcccggcca gggcgcccaa aaagtgggga
     2341 tggtcaagga tttatacgat gcctacccga ttgtaaaaca gctctttgaa gaagctgatg
     2401 acgcattggg gttctctctg acgaaacttt gctttgaagg tcctgacgaa gaactgatga
     2461 agacctataa tacacagccg gccatcctt
//
LOCUS       DAWDXZ010000255         2480 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8282_length_2480_cov_7.952165, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000255 DAWDXZ010000000
VERSION     DAWDXZ010000255.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8282_length_2480_cov_7.952165"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          594 a          683 c          671 g          532 t
ORIGIN      
        1 tggtattatt acagcgccat ctttttagtc ggtttcatcc cctgggtctt taccctgcct
       61 ctggcttggc gcgactacgg ccttacccgg aaaatggcag ccctttggaa ggaaaagaaa
      121 gtgcgccttc cggccctttc tatgaagact cagttcctcc tcgtctgggc ggtcacgatt
      181 ttcgtcacct accagttcat ggcgacgaag tacatgacct acaccttccc gtacatgatt
      241 cccattgcca tcggctttgc gtcctatttg aagaaatacg accgcttcgt cgtcaacctg
      301 gccggcatcg tcctggccct ttacgtcgcc gtgacctacc tggtcatcat tcctcagtgc
      361 cagcaggctt cggcttacgg cgtctcccag atcgtccgca ccgtagccga tgaccagacg
      421 acagtcgtca tgtacggcgg ccgctattcc gtgtcccaga cgtattactc cggccatcgg
      481 gcttaccgct tagccacagc ggaacgaatc cgtgagctcc tgcccggaga catcagctgg
      541 aatgcgaaaa atgtcatgcc tttcatggct atcgaagacc tggccgacca cgacaaggtc
      601 ctggccctgg tcaatgaccg cgaggaagcc cacttcctgc aggaagttcc cggccattgg
      661 aaatggatcg gtcaaaaagg cgtctgggtc gtttaccaaa aagaaagcga tttgtagtcc
      721 ccttggaatg gccttgtgac tgaattgtaa aggccggcgc gtatactata gtcaatctta
      781 aatgaaggga ttgatatcta tgaaaaagtt tcagaatata gtacgcatgg cgttagtatt
      841 ggctgttacg acggcaacct tcagcggcgc tgccctgacg gcccaggctg cagaaccgag
      901 accgtacgat cagcagtggg aacagcacga taacgaccgc aatcgtgaca accacaacga
      961 taagaaccac aaaaacgaca aaaatcacaa gaaatacaag aacgacaaga agtacaagaa
     1021 cgattggaaa gaaaaagaaa atcctaaaaa agatgtcgac caggccaacc gtaaagccaa
     1081 tatcgccatc ggcgtcgcgg ctatcgcaac gatcgtcgcc ttgtcgaaat aaggtcgatt
     1141 ccaagactgt ccatatcggg cagtcttttt ttgtgcccgg aacctttggg ggccgtcctt
     1201 gtcaaggggc ggtccgatgg gtataataaa atagtatata acgataaagg atgtgtttgt
     1261 tatgcaattg acgccgcgcc tggaggcggc cgtgtctctg attcctaagg ggacggtcat
     1321 cgccgatatc gggaccgacc atgcttatat tccggtccat acctgtgaaa cggggtggtg
     1381 cccgtcagcc attgccgccg atatcgtgcc gggaccgctg gcggcggcca aggctcatgt
     1441 ggccggtgcc ggcctttcgg accgtatcga ctgccgcctc ggtgacggcc tgacctgcgt
     1501 tgaaacaggg gaagcggacg gcgccgtcat ctgcggcatg ggcggttcct tgatgatcaa
     1561 aatcctgcac cagtcctatg acctctggca gtccttgaag ttcgtcgtcc tccagcctca
     1621 aagcgacagc ggcgacctgc ggcgctttct ctatgaagcg ggctggcata tcgaagacga
     1681 aaaaatggtc atcgacgacg gccgcctcta cgagctgctg cgggccgtgc cgggaaaggc
     1741 agaggctttg ccggactggc agtatgaact ggggcccgtc aattggcagc gccgcgacgg
     1801 actcctcgtc cgcaagatcg agatcctcat ggagacgaag gggcgcattt tagcgggcct
     1861 caagaagagc cgccgcgacc tgcacgttca aattgaagca ttagaaaagg acttatcctt
     1921 ttggagggac atgaaatgtc agttacagta ggtgaaatcg taaaggtatt gaatatctgg
     1981 gcgcccttat cgctgaaaga gtcgtgggac aatccgggcc tgctcatcgg aaatcccgat
     2041 gaaaccgtcg ataaggtctt ggtgaccctg gacgtcatga tggacaacgt cgactatgcc
     2101 attgaacaag gcgtgaaatt gatcgtcagc catcatccgg tcatcttcga cggtctgaaa
     2161 tcgctgcgga ccgatacgta caacggggcc atgtaccaaa agctcctggc ccatcacatc
     2221 gccgtctaca gcgcccatac caatctcgac agcgccgacg gcggcgtcaa tgacgtcttg
     2281 gctactttat tgggccttac ggacatcgaa ggcttggtgc ccgtttccga agacaaactc
     2341 tataaaatcg ccgtctacgt cccggaaagc cacggcgacg ccctgcgcaa ggccttggct
     2401 gacagcggcg ccggctatat cggaaagtac agtgactgtt cctttacgac gaagggcgaa
     2461 gggcggttca aagctcatga
//
LOCUS       DAWDXZ010000256         2474 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8298_length_2474_cov_7.804051, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000256 DAWDXZ010000000
VERSION     DAWDXZ010000256.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8298_length_2474_cov_7.804051"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          576 a          675 c          617 g          606 t
ORIGIN      
        1 cggcaacagg gtcggcattc ttagccgcga tgattacttc tgccgactct tcgccgattt
       61 tcttgcagat tttatcgatg ccggtttgaa gcagatacga cgtatacgat ttttccgtcg
      121 gatgaacccg cttatcgctg atttcccgac tgaggtcaga caagatggac aggcttccgg
      181 tgtagctttt ttcccattct gtcatggacc ggaagaagca cgatttatgg cccgtatggc
      241 aggctgcgcc gacttggtcg acagtcagca aaatcgtatc ggcatcgcag tcgacggcga
      301 tggagcggac atgctgaaaa tggcccgacg tttccccctt atgccacaat tcatttcggc
      361 tgcggctgta ataccaggcg tcgccgcttt caacagtacg ccggagcgct tcttcattca
      421 tataagctac catcaggatt tttccgctgc atccttcttg aacgacggct ggaaccaatc
      481 cggcgccgtc aaattttata tctttcatcg atacactatc catttatact tctgcccctc
      541 tcatgggaat cccctgttcc tgtaaataaa ctttcaaatc gggaatatcg atttgtccaa
      601 agtgaaacac cgaagctgcc aaggctgcat cgacaccggt atcgaagacg tctttaaaat
      661 cctgcatcgt gccggctccg ccggaggcaa tgatcggcac gggaagttca gaagccaaac
      721 ggcggttcaa ttccaaatcg aacccggcct tttctccgtc ggcatccatg gaattcatgc
      781 agatttcacc ggcccctaaa gcaacgccct gtttggccca agccatggcg tcgatgccgg
      841 tattcttacg tcccccttcg acgtatacct gccagctgcc atggttgtcg cgcttggcat
      901 cgatagacaa gacgacacac tggctgccaa aacgctgggc agcctcgcga atcagttccg
      961 gccgccgaac agccgccgag ttgaccgata ccttatcggc accggcccgc aagacttggt
     1021 gaaagtcgtc gatactggca ataccgccgc cgatcgtaaa ggggatagtt atcgtttcgg
     1081 ccacggcgcg aacgacgtcc aggaagatat ggcgtttttc atgggtcgcc gtaatatcgt
     1141 aaaagacgag ttcgtctgcc agctggtctg aatagaattt ccctaagctg accggatcgt
     1201 caacatcctg caggtgctta aattgcttgc ccttcacgac gcggccgttc cggacgtcaa
     1261 ggcagggcac gattcgtttc gccaacatct tacattcccc ctaaacttcg gatttccgcc
     1321 atggtaattt tcccctcgta caaggctttc ccggtaatgg caccgtagat gcccatggcc
     1381 ttcagttttt ttaaatcgtc gatgctgctc atgccgccgg aagcgataag gcgaatataa
     1441 ggcagctgct ggagctgacc aaggacggta aaattcggtc cggtcagcat cccatcacga
     1501 ctgatatcgg tataaatcaa ggtattgatg cccaggtcca gcatcgattg ggcaaaaggc
     1561 aaaacgcgtt tatcgctgcc gtcgacccaa ccgcgggtgg ctacgacgtc ttccttggca
     1621 tcgatggata cggccagcat ggagccgtat tttttagcca tggcagccat aaagtccgga
     1681 tcttcgacgg ccttagagcc gatgatgacc cgattgacgc cggcctctag ataggtttca
     1741 atcgcttctt cactgcggat accgccgccg acttcgacgg ggatatcaac ggcttcacaa
     1801 atatcgctga taatagaagc gttagcgagg ctcccggaaa aagcgccgtc aaggtcgacg
     1861 acgtggagaa agagagcccc ttcgtcagcc catttaacag ccgcttcgac aggattgtca
     1921 aaataggtcg tactgtcatc ttttacgcct tggcgcaagc ggacggcgcg gccgttttgg
     1981 atatcaatag cgggaaatac aatcatggtc tcatccttcc tgtttcacat aatcgacgac
     2041 attttgcagg agctgctgtc ctacgcggcc gctcttttcc ggatggaatt ggaagccaag
     2101 gacgttgttg aggccgcaga tagccggtac cggttctccg tagtcggccg tggcaatgac
     2161 gtcaggcgtc gcagccggtg ctttatgata cgaatgaacg aaatacacgt aaggctcttt
     2221 gggaagattc tttttgagcc agtggtcctg acgaaattcc aaggcattcc agcccatgtg
     2281 cggtactttc aatttcgtgt gaaagggtac gatatcgccg ggcagaaaac ctaagccctt
     2341 atacgttccg ccttcaaaac tctgttcaaa taaaagctgc attcccaagc agacacccat
     2401 gaagaatttt ccggctttta cttgttcctg taggagagga atgaggtcaa atttttctaa
     2461 gttcccgatg gcat
//
LOCUS       DAWDXZ010000257         2472 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8303_length_2472_cov_8.136533, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000257 DAWDXZ010000000
VERSION     DAWDXZ010000257.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8303_length_2472_cov_8.136533"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    350..380
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          500 a          626 c          701 g          614 t
ORIGIN      
        1 aaataccgct tccctgcctg caagatgcgc aaaaagggac ctgacgcagc aggccctttt
       61 cttggcacat attattgtaa aatatagacc ggtaccatgc ggcggccaaa ggccatgcaa
      121 acatcgtagt cttccatgca gagatcgatg cggttgccga ggatggcccc gcctgtatct
      181 tcggcacgag ctacgccgta accgggaatg tataccgtcg agcctaaggg tatgacgttg
      241 gggtctacgg caacgacgcc atagcgggca cgggttccca tgcgggtaat ccccaatccg
      301 tcgccatcgg taggcaaata agccgtcgct tccatggcga tgactttttn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn cgttgccttg gtgcccactt tgcggaccat aggctgcatt
      421 tctgaaatga gctctgttga aatcgtctct ttatcggcaa cctgaccgtc gacgctgacg
      481 atgttcaact tcactcgctt gcggccattt tgcccttgct ggacaacttg ttcttccccc
      541 tgccctaagt tttcatcggg aatactttct acagcatagg gtacgacgtc atcttccgta
      601 atcgtcttgc gcgagacgtt gccgatgacg atgttttgtc cggcctggag gggcttttga
      661 atgtcgccgt aagggcggta tttttctttt gaatacccgt actgctcggc tacttcctgg
      721 gtattgcgct tagccgtata caccgtctgc gattggcccc tgtagacgag ggtcacggga
      781 atggcccggc gtactttgac gtaactgccg tcgtcgagtt ccgccgtcga catggtgact
      841 tcgtcatgat cccccatctc gatattatac tcacgcagca aggcatctgc attgacggca
      901 tgggtataca cctggcgggt cgtcccgtcg gccacaaggg ttaccgactt tcctttatcg
      961 atgaagccgg tcagcgagac gaggaccaat aaggcaatca tggtcgcata catctgcgtg
     1021 atcggccctc ttcgcttaaa gctcagtata ctgaattttt tccactccat gccatgcctc
     1081 ctaaaacgtt tcttaccatt ttagcaaagg ccgcaggcat tgtcaattta gttgtcgccc
     1141 tgttccgtcg gatccgtcgg acgcgggcga aggacgatat tgcgcagctt ccgcacttca
     1201 cgcttgcggt gtgccggaag tttttccgct tcacgctggc aggtatcctg ggcattaacc
     1261 gcttttcctg ttcgttcgtc agccagataa cggtacgaac cgtcttgatt catggcgtag
     1321 gctttgacgt tgtcatcaaa atagagtttc aggatatgtt taatctgttc tttatgaggt
     1381 gcaaattcga caggtgtcat caattcgacg cgttcattca ggtttcgcgg catccagtca
     1441 gccgaggaaa tgaagacgtc ttcactgccg ccgttataga agtagaataa gcggctgtgt
     1501 tccaggaagc ggccgacgat gctgtggact tcaatgttgt cactgatgcc ggggaggccc
     1561 ggacgaagga cgcagatacc gcggacgatg aggtggatct tgacgccggc tgccgaggct
     1621 tcatagagtt tagcaatgac ttctttatcg aggagcgaat tcatcttagc gacgatgagc
     1681 gcttttttcc cggctttggc ccaagcgatt tcctggtcga tttcctgata aatggtttcc
     1741 cgcaggttca acggtgccac agccagttta ttccattccg gaggatcgga atagcctgac
     1801 aagaggttaa agaaagccga cgcgtcgact ccgatgaggg tattagacgt caagataccg
     1861 acgtcagtat agatgcgtgc cgtcttgccg ttatagttgc ccgttgccag gtggacgtag
     1921 cggcggatac cgtcctcttc ccggcggacg acgagggtga tcttcgagtg tgtcttgagc
     1981 cccataatac cgtaaatgac gtggcagccg gctttttcca gcttacgggc catgagaatg
     2041 ttgttttctt cgtcaaagcg agccttgact tccatcagga ccgtgacctg cttgccgttt
     2101 tcggccgcct gagccagggc tgcgatgatc ggcgactgac tgctgacgcg gtacagggtc
     2161 tgtttaatgg ccaaaacgtc gggatcgata gcggcctgat ggatgaagta ttctaccgtt
     2221 tcaaaggttt catacgggtg gtgcaccatg atatcccgtt cggcaatggc cgaccagata
     2281 tcggtactgc ccatggccgc cagttccgac gacggctgtg gttttaccgg cggatagcgc
     2341 agtccgtcga agccttcgat gccgacgaag gagaagaata ccttgcaaat agggggcgtc
     2401 gggaagaagt ggaaccagtg gggggcgggg gggggggtgt gggggggtgg gggagggggg
     2461 gggggggggt gg
//
LOCUS       DAWDXZ010000258         2467 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8317_length_2467_cov_7.101990, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000258 DAWDXZ010000000
VERSION     DAWDXZ010000258.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8317_length_2467_cov_7.101990"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    275..284
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1869..1878
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          507 a          714 c          674 g          552 t
ORIGIN      
        1 agcggcgtat ctgacacggt cagctgacag acgctgtcac cggtacgtat cgtttccagt
       61 tgggcgatgt atgtataagg cgcaaggccc atcatggcaa agggataatc ctgggccggg
      121 aaaaagccgg aaagggtcag tgtcttgagt cccgtctttc ccatcataag gtagtcgccg
      181 aagttattga tgttcaccgt accatgattc gtattgacag ataccatcag ctccgaaggc
      241 aggacgggaa agaccaccgc tgccgattca gaagnnnnnn nnnncctgct cgctgcggac
      301 gttcatggta tcgatgcgga tggtgatgcc gccgctgcct gcgttcatgg cctggcggag
      361 actttcatca tggggtacga ccgtactgcc gttgggcagg tgtaccagtt cgccccggcg
      421 atcttcattg atgacggcaa agccgccacg aaagttctcg acgccgcttt caaagtggct
      481 gatgcttggg atatcaaatc ccacatgggt cggcgccccg cccgtcaggg acggaatgtc
      541 gatggacagg ccgttgacgc tggcaatgag tccgttcacc tggtcgatga cccagttcac
      601 accgctccgg aaggtatcct tgatgctctc ccagatactg gaagccgtct cgctgatacc
      661 gttcatggcc ccgtcccagg cagaactgat ccagttcatc cccgcaccga cggcgtccga
      721 tacagcctgt atagcctgtt cgatataatg cgacacggta tcccagttcc tccacagaag
      781 gtacaaggca gcaataatcg cagcaatgac gataatgatg ggattggcca tggctgcggc
      841 gcctacggca cggataatgg tgatcatcat gcgtccggca gtcagaaagg tactgcccat
      901 gcccctggcc acgatggcaa tgccccggca gaccggaatg agtcctttga actgggtcga
      961 gaggtatttc gagacactgc cggctttgct gatgccggta gcgatagagt taaaagtacc
     1021 aaaggccctg ccgccgaccg tcaggatacg gcccaaagtc gagccgaaga gctggaaggt
     1081 cacgatgccg aaagccacct ggccaatcag cgttttctgt tccggtgtaa gcgcacggaa
     1141 ccaggccgcc agttccttga cgcgcatcga catggccttg aagtacggcg taaacgacac
     1201 cgctaaatcc atgccggcat tcttcagctg gttcatggca atctgcatct gctccgacgg
     1261 ggtcagcatc ttctcataag cttcccgggt catgccggca gactgggcca tctgattcat
     1321 gaccttgtcg aaatccccgg ctcccttgcc tgtcaggacc aggatgctgt tcaatccttc
     1381 gacggagcca aagagctggg ccatctgctg ggcatcaccg cccgtggccc gcttcacttc
     1441 gcccaggaac ttcacccagc ctacgctctg cagatgggcc gcattgaatt caagaccaag
     1501 ggactgagcc agtttcgccg cttcagaaga cggcttcaga atgttgctgt aggccgcctt
     1561 gagtcctgtg atggcttcac tggtccggat accgttcttg gtcaggacgg caatggaacc
     1621 aaacagttcc tgcgtgctga cattaagctg tgccgcaatg gggatgacgt tgcccatgga
     1681 ctgggccatc tcgccaaagg atgtcttgcc gaagttctgt gccaggagca tctggtccgt
     1741 caccgccgtg gcttcctctg ccgatttccc ataggcattg aggacggtcg tgacgccgtt
     1801 tacggcagtc gtcgtatccg tgaacccggc cttggcggcg atggtcatat ccttgacaaa
     1861 gcctacggnn nnnnnnnntg gccggcatcg acacctgccg agatggcctg gtagaccgat
     1921 tccgaaaggt cggcgacgcc tgccccggtc tcatcgctga cagcacggat ctcatcactg
     1981 accttctgca tggaaacgac tgtcgtatcc accagggtcg aaatcttggc gatgccgccc
     2041 gcaaagtcgc tgtgcaactt gaagcctgct gtcgcagccg ccaggatggg tgccgacagc
     2101 agggccatct tgtctgacag gccggaaatc ctgcttcccg tctgctcgat gctctttgct
     2161 gtccgttttt ggatgcgctc atgctccgtc agcttgtccg acagaccact gaccgattgt
     2221 ttcgccgccc ccatctgggt cttcatggtc cccaggctgg cattgacgct acgcaccgtc
     2281 ggcgtgaaca aatcccgcag ccggatggcg gcatcgatga cattattggc catgcggttt
     2341 cacctctctt tccatcatct gccttattta tctgcctcat tctcttgcta tctgccacat
     2401 ttcatgctaa aatgttgcag ataatagaaa gatgagggag atttttatgc ggatatttaa
     2461 ctactcg
//
LOCUS       DAWDXZ010000259         2462 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8334_length_2462_cov_6.825924, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000259 DAWDXZ010000000
VERSION     DAWDXZ010000259.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8334_length_2462_cov_6.825924"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1980..1994
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          547 a          656 c          639 g          605 t
ORIGIN      
        1 tcattcaggc cggtgccgag ccggtctatt tggagactgc cagaaaccct tatggcttca
       61 tcggcggcat cgatgaccac tgctttgatg aaggatatct gcgccagctg gcagccgaac
      121 ggaaccccga aaaggcaaaa tccaaacggc ccttccgctt ggccgtcatc caactcgata
      181 cctatgacgg cacgctgtac aacgcccggt acgtcgtcga ccgcatcggc cacctctgtg
      241 attatatcct ctttgattcg gcttgggccg gctatgaaca gttcattccc atgctcaagg
      301 actgctcgcc cctcctcctc gacttgcatg acgacgatcc gggcatcctc gtcgtccagt
      361 cggtacataa acagcaggcc ggtttttctc aggcttcgca aatccataaa aaggataatc
      421 acatccacaa ccaggctcgg tactgtaacc ataaggtctt taacaattcc tttatggcca
      481 atgcctcgac gagtcctttt tatcccatgt ttgctaccat tgccgtcaac gccaaaatcc
      541 acgccggtgc cgccggccga aagatgtggc tggactgcgt gaaactgggc atcgatgccc
      601 gaaaagatat cctgcgccgc tgccattata tccggccctt cgtcgcacct gccgttcacg
      661 gaaagccctg gcaggagggg gatacggatg agatgacaca tgacttggcg tactttacct
      721 ttgagcccgg agccacgtgg catgcctttg aaggctatgg accggggcag tacctggtcg
      781 acccgtgcaa gctcttactt acgacacccg ggatcgatgc tgaatcggga gaatatgaag
      841 atttcggaat ccatgccaat attctggcga cgtaccttcg gggaaatggc gtcatcccgg
      901 aaaaatgcga cttgaactcg atcttgttcc ttttgacacc ggcagaaaat aagaacaaga
      961 tgcgcctcct cgtatcccaa ttggtccgct tcgaacgctt cgtcgaccac gacgtcccca
     1021 tgcaggaagt cctcctcggc gtctacaatt cccatcctga acgctatgcc ggctatacga
     1081 tacgacgcct ttgccaggaa atgcacgatt tttataaatc ccatcatgta aaagatttac
     1141 agaaaaaatt gttccgtcgg gaatacttcc cggaatacgt catgagtccc caagaagctc
     1201 atcatgcctt cgtccgcggg cagggcgaat tggtatccct cgacaacatc gaaggccgca
     1261 tcgcccttga aggggccctt ccgtatccgc cgggcgtcct ctgtgtcgtc ccgggtgaac
     1321 gctggtcaaa gacggctgtt cagtatttca aggccctcga agaaggtttg aacgaactgc
     1381 ccgggtttgc ctcggaaatc cagggcgtct atttggaaaa agaagacgac ggccgcatcc
     1441 acgcctatgg ttacgtccta aaagaataag cttgtacatg aaaaaaacac gattccttcc
     1501 ttaagcagga gaaatcgtgt ttttgattga ttatcttaca agaggttttt gacggcatcg
     1561 gagatccgtt tggcgatgtc cggattattc atggtgtaga cgtggatacc gtcgacgccg
     1621 ttggccagaa ggtcggcgat ttggtcgatg gcataggtga tgccgatatc gtacatggcg
     1681 tccggtttat cgccgtattt ggcaatggcc cgctggaatt tgatgggcag ggtagcgccg
     1741 cacatggcaa tcatgttttc gatttggcgc ttattggtga ccggcatgat gccggcttcg
     1801 atgggaacct tgatgccggc gatttcacag cgttctagga agcggtaaaa tacggcattg
     1861 tcaaaaaaca tctgagaaat cagttcgctg gccccggcat cgaccttggt ctttaagttg
     1921 cggatgtcgg cggcgacggt cttggcctca gcatggcctt cgggatagca ggcaccgatn
     1981 nnnnnnnnnn nnnnctccgg agccttttct ttaataaagg cgatgaggtc gctggcgtgt
     2041 tcgaagcggt gcgttcgttc tcttcccggc gtttcgtcgc cgcgaagggc caggatgttt
     2101 tcgatgcctc gagccttcag ctcctgcaat gttttaagtg catcttcttc ggtcagatag
     2161 atgcagggca tgtgggctac tgtttcacag cccaggtttt ttatatatgt tgcgacttcc
     2221 gtcgtgcggt tgccgccgtt gccgccggct ccacaggtca cgctgatgaa gtccggatgg
     2281 atcttgctca gttcatcgag cgtcgggtag acggtttcga tgggcgtatc tcgctttggc
     2341 gggaaaactt cgaatgaaaa gaccgtcttt tttgcaaaaa gctcatgagt atgcatacat
     2401 taatttcccc ctcagatcat aagaagataa agggctgccc catagaggca gcctcttttc
     2461 ta
//
LOCUS       DAWDXZ010000260         2459 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8345_length_2459_cov_7.451747, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000260 DAWDXZ010000000
VERSION     DAWDXZ010000260.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8345_length_2459_cov_7.451747"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    823..832
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1449..1458
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2182..2191
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          476 a          647 c          684 g          622 t
ORIGIN      
        1 tagcctgccc gtccttttcg gactgggcaa tcctgacgat ttcttcatca ctcatgcctg
       61 caaagcggtc atttttttca gtttcatgag acgctgcctg cattatcgtc acctcactta
      121 tttgatggta ttcgcctgac gctggcgcac gacttcatac atgagcagtg ccgctgctac
      181 ggaggcgttt aaagaattga cgtggccaaa catgggaatt tggaccagga cgtcacaggt
      241 ctccttgacc aaatgagaaa tcccccgtcc ttcacttccg atgaccagga cgacgggatc
      301 ggtcaaagag ctgtggaagt aatcggtttc cccctccata tcggcgccga taacccaaaa
      361 cccccgtttt ttgagttgtt tcagcgtctg ggaaatgttc cctatctgca ccagcggcag
      421 gtattcgatg gccccggccg acgtcttgcc caccgtttca ttgatcggtg cggaccggcg
      481 tttcggcaag aggacggcat cggcaccggc acattcggcg gtccggatga tggcccctac
      541 gttgtggacg tcttccaagc cgtcgaggag aaccaaaaag ggaaccttgc ccttttcatc
      601 ggcggcgatg aggatgtccg tgaggtcggc atattcaacg gctttggcaa aggccacgac
      661 gccttggtga cggttatccg gcgccaggcg gttcaagacc tttaacggcg tctcttcgac
      721 gaggatatcc gcctcggctg ccaagtcacg aatccgctgc aagggcttcg tttcattgcc
      781 cgacgtgacg tatatcttgg taatggaccg gccggccttc aannnnnnnn nnttgcggcc
      841 gatgatcagt gtatccgcgc tcattcctta cctcctctgg tttggctgca gtaagccttt
      901 gtttcttcat aaatctgttc catgatctca gccaaccgtt gttcctgccc ggccatgact
      961 aaaaagccga ctaatgcttc aagggccgta ctgtcatggt attcggcaac ggtagcactt
     1021 ttaggcgcca tagtatgggc gttccgcgcc ctttgacaga cgcttttttc ttcttctgtg
     1081 aggctatcgc gaaggcggcg gtagacccag gcctgggcct tagccgacac gaattccgca
     1141 gccaggccgt ggaggacctg tacctgggga atccccgttt ccaccaggcg cttgcgcagg
     1201 gtcagggcat aataagcgtc gccgacgaag gccaggttga tggaatcgag acgggcggca
     1261 tcgctttcag ggacatcgag gcgcttgcct tcggcaaagg cctcgtaggc ccgttttttt
     1321 aaggcttgga gttgtttgaa tttcactttt tccagcgtgc tccctgggga gaatcttcga
     1381 tggtgatgcc gagggcggcc agtttattgc ggatggcgtc ggccagggca aactgtttct
     1441 gaacgcggnn nnnnnnnntt gctgctggca ttggctttct ggccttccca gcgttcttcc
     1501 aggatgccga ggatatccgt aaaggtcttg aagattcgtt ttacttccga cataatcgcc
     1561 gaatcgacgg gaacgttgtt ggtgacgacg gcatttttat agatattgac gttcttcgcc
     1621 aagtcgaaca tggcagcact ggcaagtgcc gtattgaaat cgtcgcacat ggccgtttca
     1681 aagtcgtctt cgtccttctt agctgccgcc aagagctgtt cggcttcggt cgatttgctg
     1741 ccggccggca tttccatgag ttccaagacg ttgtcgatgg ccgtgcccaa gcgttccagg
     1801 ctgcgcgtca tttcgtccag gcgttcatcg ctgaagtcca aggggctgcg atagtggttg
     1861 gaaatgagga agaagcgcag ggcatcgggg ctgtaatgag ccaggacgtc ttttaccagg
     1921 aagaagttat tcaaggactt actcatcttt tccgagttga tcgtaatgaa cccgttgtgc
     1981 agccagtatt taaccatcgg tttcttgccg gtgtaggctt cggactgggc aatttcattt
     2041 tcatgatgcg ggaaaatgag gtcgctgccg ccgccgtgga agtcaaaggt atcgccgagg
     2101 tacttatggc tcatggccga gcattcgatg tgccagcccg gacggccttt gccccaaggg
     2161 ctgtcccatg ccggttcgcc cnnnnnnnnn ncggttggaa atcgtatccc agctgacgcc
     2221 ttccccgttg gcagcgttga tgatcttgtc atcgacgtcg gtgaaattct ggatatgcgt
     2281 gacttcgtag ccgaggtgtt ccaggtagcg ctggatgacg tcccaggtta cgaagggacg
     2341 ggcattcccc atgtgggaat ggttatacgg cgtaatgcca cagacataca ttttgacctg
     2401 gcccggcgtg acgggaacga aggtctcctt tttacgagtc agcgtattgt atactttca
//
LOCUS       DAWDXZ010000261         2457 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8353_length_2457_cov_5.927560, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000261 DAWDXZ010000000
VERSION     DAWDXZ010000261.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8353_length_2457_cov_5.927560"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    772..781
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2161..2170
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          653 a          663 c          531 g          590 t
ORIGIN      
        1 ggaaaagggg tcttgtcctt ctaagagtcc ctggagaaaa taaggaataa acatttcgct
       61 gacaacttgc ttgtcatagt gccgctcttc gttgccgtcg gacacggtaa ttcccgttcc
      121 gattgccagt cggacggcac acttcggact gcccgtcggc aagatatccc cgtgagccca
      181 aaggacggtc gccatagccc gccaaggata gggatcgtct tcatcctcgt cgcattcggc
      241 aacttctgcc gccacgatga gtccgttttc ccgagccagt tccgccgtat ggaagacgaa
      301 ctgtgtatat tcatcttctg acgtcgccgc cgcttccgaa atatacagca aggggaaatc
      361 ttcccataag acgtgttccg aaataaattc ttccgtcggc gcaatatcgg cttcttcttc
      421 gacaaaagac gtatgcaggt tgtaagccgc acaaatctct tccgcattgt cgccggcgtt
      481 gagcggatag acgtgaaacc ctattttttg gaacccttcg gtcacgtgaa tatccagccc
      541 gtgtttcgat tcatcttcct gccaaaagac aaagggcagt ccaatgtata tcacatccac
      601 tattatcacc tctatactta tagtattcga cctgatctgt ctaaactcct acttctacaa
      661 tatatattct tgtattgtct gtcaattcct atataatata tttatagcta atacggcctt
      721 tctaaaaagg ccagaaagga agtatgcctt atgttcaaaa aaacggccag tnnnnnnnnn
      781 ncgttcgcta cgatccgccg tactacatca tccgggcgac ggtcgtgacg tacgattatg
      841 ccaacaatac cttacaagga tatgataaca attttttcta caattttaaa tcccaatcgg
      901 taaaaacgca gaccttaggc gacgtcggct acgaccctgc cggtaatatc ctgtctaccc
      961 tggccctggc taacccggaa gtcaagacga cagaccgcct ctcggccaac ggcaatgcag
     1021 ccaatcgtgc cttccgagcc tgctacaaca tgaatttttt ccaagaataa tacactgaaa
     1081 aggattgata gttatggatt ccctcatcca catcgtaacc accgtcgcga ccatcatctt
     1141 catcggcgcc gttctctact ccctgacgtc ggcccttaga aaaaagcggc gcaaggcaaa
     1201 gtatgaaaaa gacctggtga ccaaggtcca cgtaaaagtc gacaatctcc accaatacga
     1261 catcaaaccg gaaaagggct actacaagta tccccttgtc gaaaagggaa aactccgtcg
     1321 cttcccccag cactttacga gtatcgccat cgagcttgcc aatgagcagc cttactccat
     1381 ctgtctcctg gctctggccg aatttgataa gggcgagata aaagacacgc ggtacttcta
     1441 catccgtccg cctgaaaaca acttgtccgc tacgggaggc cgacatcgct gggaagactt
     1501 gtctaaagcc gatgagttcg gcgaatactg gcaggccggt cttgaaaaac tcatcgaagg
     1561 caagaccctc atcgcccaca acgccccctt cgtcctgggc tgcctgaccc atgcccttca
     1621 catttacggc atcaaggccc cgtccttccg ctatgccgac acgatggaag ccgccaaaaa
     1681 atactaccat ttctcttcca ataagatcga aaacatctgc gatgaaatgg acatcaagac
     1741 cgaggaaggc aatcttctca gcaaggccat cggaattggg aaattcttcc tggaggccaa
     1801 gaacgactat cctctctacc tgccgtctct gacctacatc gaaaaagaac cgaccgatga
     1861 cgatcgaaaa gcggcctaca tcgccgccgt cgaacgtgaa gaaggtacgc cggaagaaat
     1921 ggcggccccg gaaaaaccgg atttggctct cgttaaagac ctggtcgaca cgggatgcat
     1981 caccgcgggg aaacagtccg gcacctacta tgctgccgac aaaggccttg ctctattaga
     2041 acaggccgac tgacttcaaa ccgaattctt attttgcaca tcaaaaaagc gctgtctttg
     2101 aaagacaacg cttttttatc atactcaaat aattcttatt tggagtacaa ttcgacgatc
     2161 nnnnnnnnnn gttgatttct acagggattt cgtcgcgttc aggcatacgg gtaagggtgc
     2221 cttcgaagcc atcgaaattc ttttcaacgt agggaacgtc gaatgctttt aattcctgga
     2281 aggatttctt gtacatttcg ttcgaacggg aatcttcttt caatgtgata accgtaccaa
     2341 cttttacgtg tgccgacgga atatcgcagc gtttgccatt gaccaaaatg tggccgtggt
     2401 tgaccatctg gcgtgcctgg cggatagagt tagcaaaacc gatgcggtat actaagt
//
LOCUS       DAWDXZ010000262         2451 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8370_length_2451_cov_6.682805, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000262 DAWDXZ010000000
VERSION     DAWDXZ010000262.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8370_length_2451_cov_6.682805"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1284..1293
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          624 a          646 c          658 g          513 t
ORIGIN      
        1 cgataccttt accttgggcc gcgttttcag tacgcctgtt gcgattgaga acttcgtcgg
       61 catttggcag atgcggcgcc cgcgcagtat ggctctcgcc gccggaacgg tctttgaaat
      121 ccataaagac ggtccctggc aggataagga tgtccaagct ttgacacagt tgatgtacgg
      181 cggggccggc gaccggacgg aagaaggatt cggccagatg cggcggtgga aactccaaga
      241 cctgactatc gatggtgaga gcgaaaagaa ggacgtggaa gcaatccgca tcgattcgct
      301 agaagtaaag aaacgagcgg ctgctatctt ggaaagccgt atcaaggaac agctccgcaa
      361 ctttgcctat gaagacgcag ctcaattaaa gggcctcgaa ggcggagcca agacccatct
      421 ctttgcccgc ttagaatcgt ggctcgaccg ggatttccga gctgaactca aggccaaagc
      481 cggcaagaat gagaccgtgg gtactcatct tcgccgcctg cgcctgtacg gggcgcctct
      541 ctacgagatt tttaacgggg ccaaaccggc gccttatgac agcccggaac ggaaagatct
      601 gttaaaggag cttattccgg acgaactcgc tgacctgatt ggattcaagg agcccaaaga
      661 atcgtatttt catgaatact ggctctggtt cttccgccac agccgcaaac gggccgtaca
      721 agtccgtaaa actgcatcgg aggtgaagaa cgatgaaaca tgaagccagc ctgttatact
      781 gcgtgcaagt cgaagggacc ttatccctta aaaccccctt actcatcggt gccggcaagg
      841 aaaaggataa tacgactgat atccacgttc tgcgcgataa aaaaggacag ccttttatcc
      901 ccggcacgtc gcttgccggc gtcctccgcc atcttgccgc taactgggat aaaacggcga
      961 cgcagctgct ctttggctac agtgacgacc gccgcggaga aggcgtgcag agcgccgttt
     1021 gcattgacga tattcccctt gaaaacgctt atattaccgt tcgtgacggc gtttccatcg
     1081 acgaatacac gggcgttgcc aaagataagg ccaaatacga ctatgaagtc gtcgaaagag
     1141 gggctcgcgg caccttcacc atgacctggc agctgcggca gaaagttgtc gacgacttgc
     1201 cgaaatggaa ggtatttctt gacgatttag taaagcaatt atcctatggt atccgtctcg
     1261 gcgccatgac ggccaagggg tttnnnnnnn nnntgaaaag gccaaaatcc atctctacga
     1321 ttttagccaa tacgacgatg taaaggcctg gctcagtcgg aaggttccca aaacggtcta
     1381 tgacgtcaag gccgaagaac gcttaggcaa ccagtctttc gtcatcgaag ccgattttgc
     1441 cttgaagaca tccctcttag ttcgcaaccg tgacgtatcg gccgcagaca gtgatcagaa
     1501 tatcgatgtc gttcccatga tgagcggcaa cgattacctc attcccggcc cgtcggtcaa
     1561 gggcgtcctt cgccatcagg catctcatat cctgcaggta ttaggaaagt cgacggatct
     1621 attggacggg ctcatgggtt actcccgtga cgatgggaaa aagcagaaga gccgtttcct
     1681 cgtcgacgaa gtctatattt caccggacca cgtggcagcg gttaaacaga cgcgcaacgc
     1741 catcgaccgc tttacgggca gcaccatgga cagcaaactc tttgccgaaa agcccttatg
     1801 gcaaaaagat aagatccgct ccgtcgtcac cctgcgtctt gccattgaag actgccaaga
     1861 ttgggaagcc ggactggctt tgttcctctt aaaagatctc tggaccggcc aagtggctct
     1921 gggcggcgat aaatccgtgg gccgtggcta tttgaccggc caccatgccg acatccgctt
     1981 ccaaaaagac ggaatgcgcc agcactgggt cctcgaagag aacggaaagg taaccggcgg
     2041 tgatccggca gccttagaag cctatgcagc ggcactgaat cgtgttgcgg aaggggagtg
     2101 agcgcaatga gtttggaatt agcccaaaag ggattgaccc ttgtcgacag ccccgtgact
     2161 tggaagcgaa aaatccatga agcacagcat ttaggggacg tcatcaaaca atatatgaaa
     2221 gattcggcca gcctcgtcgt atggcagcgt caacagattt cctggggccg ttgggacggg
     2281 tcatcactcg tctttgccaa ccaaaaaacc ttggacgaaa atcagattct ggaaatccgt
     2341 gtctttaacg aaaacgaaga acttcatctc taccgccgtc acggtgccta tgaaggccga
     2401 tatgtctgcg acggcgaagg atcggtccaa agccacgtcg acgccttatc c
//
LOCUS       DAWDXZ010000263         2446 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8389_length_2446_cov_7.306566, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000263 DAWDXZ010000000
VERSION     DAWDXZ010000263.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8389_length_2446_cov_7.306566"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2026..2080
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          592 a          686 c          616 g          497 t
ORIGIN      
        1 cgtcgtcatt tccatcgtcg gctatccgaa agacgtggaa gaagtctatt ttggcgataa
       61 aggcatctta aaggggaaaa aaggcggcac cgtcatcgac atgacgacgt cgagcccgtc
      121 ccttgctaaa gaaatttacc acaaggcaaa gtccctgggc atcgacgccc tcgacgctcc
      181 cgtctccggc ggtgacgtcg gcgctaaaaa cgggaccctg gccatcatgg tcggcggcga
      241 agaagcagcc tttgacgcca tgaatgccgt cttttcggcc atgggcaaga ccttgactta
      301 cttcggcccg gccggcagcg gccagtacgc caagatgagc aaccagatcg ccattgcgtc
      361 caacatgatg ggcgtctgtg aagccattgc ctacgcccaa aagagcggcc tggaccctga
      421 aaaagtcatc gccaccctct cgtccggcgc tgccggttcc tggtccctgg ccaatttggc
      481 accgcggatg gtaaaaggcg atacggctcc cggtttttac atcaagcact tcattaaaga
      541 catgcgcatc gccctcgaat cggctgccga aatgaacctc gacctgccgg gcctgactct
      601 ggccaagaag ctctacgaca aactggctgc cgacggcatg gaagactgcg gcacccaagc
      661 cctcttccag tggtacgcaa atcagaaata ggcctttctc atctgaaaag gcatgcaaag
      721 gaggtgaccg ctgtcatggc ggcatcatcg aagaaactct atggtatgaa agaagcctgt
      781 gagcagacgg gccttcctta tgaaacctta aaatactact gcaatacggg cctcgtgccc
      841 aacgtcaagc ggtcggacaa taaccgccgc gtcttcaacg accacgacat cgcctggatc
      901 aagagcctga cctgcttaaa aaaatgcggc atgaccatcg aagaaatgaa acagtacctg
      961 gcctactgcc tagagggtcc cgattccatt cccgtccgca agatcatgct cgataagaag
     1021 ttgaccctcc ttgaggaaaa gctccaagaa gtccgggaat ccatcgacta catccattgg
     1081 aaacagaatt tttacgacga agtcacgtcg ggaaaacggg tctataccag cgatttactg
     1141 cccgaataca acgacgatga cgaataaatc atcttccatt agaaccgtcc gtattcgcca
     1201 atgaggagaa aaaccttgca tatcatcgaa atcaaagacc gaaccccgga attgacggaa
     1261 aaactgctct ccgtctggga aagctccgtc cgggcgaccc atctcttttt accgcccggc
     1321 gccatcgaag ccattaaagc cttcgtcccc caggccctgg ccgtcgtccc caagctgatc
     1381 atcgccgaag acgacaacgg ctctccctta ggatttttgg gaattgacga taaaaaggtc
     1441 gaaatgctct tcgtcgctga cgaacaccgc agccgcggca tcggctcggc acttctcggc
     1501 tacggcatcg aacagtacgg cgtcaacgac ttggccgtca atgaacagaa tcccttagcc
     1561 aagggctttt acgaacactt gggattcatc gtctataagc ggacggaatt cgacgaacag
     1621 ggacatgcct accccctgct ctacatgaag cggtcctaaa gaccgtcaac acccaccaca
     1681 gaagagacct tctaagattc actgtgaagc gaaccttagg aggtcttttt tcatggaaac
     1741 gcaccgtcga tttgccggcg tgccgtgatt tcatcactta ggcataaaaa aattccgagc
     1801 aatctttttt aggtcactcg gaatagagac atctgatgtt ggaggaccct ttccctcact
     1861 ttgaagggtc ctttttaaaa tggtcgctgt actgctgggt gtccggctca gcctgacggc
     1921 aggcatgggt aaaggccgga tggtacaaga aactccggtc gtacgtatcg ccgaggacct
     1981 tgccgacaac gatgagggcc gtcttcgaaa cgccgtactg ctgcgnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cttctaacag cgaggcgctg
     2101 agaaagatga ccatcgtcgc ctgatgggcg gcgtagtcgg cgattttctg cttgtccgga
     2161 acgggggtcc gcccttccat gcgggtaatg atgaccgatt ggctgatgcc gggcaaggtg
     2221 tattcggcct tcagggccgc cgcggcgcca cagaacgaac tgacgccggg gacgacgtcg
     2281 aaggcgatgt ttttttcagc gaggatgtcc atctgttcgc gaatggcccc gtagaccgac
     2341 ggatctcccg tatgcagacg gaccgtcatc cagcccagtt tttccgccgt ttccatagca
     2401 gctatgacgt cttctaaggt catggccgca ctgtctaaga tgacgc
//
LOCUS       DAWDXZ010000264         2444 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8398_length_2444_cov_11.114692, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000264 DAWDXZ010000000
VERSION     DAWDXZ010000264.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8398_length_2444_cov_11.114692"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          556 a          589 c          667 g          632 t
ORIGIN      
        1 agcattatat gcaataagcg aaagaaaaat cagatgttat gtatggaagg agaaatgtat
       61 tatgcaggac aagcagtatg taatggctct tgacgccggc acgacgagca atcgcgcaat
      121 tatttttgac aatgaatcgc atatcgtcag tgtcagccaa aaggaattta cccagtattt
      181 cccaaagccg ggctgggttg aacacgatgc caatgaaatc tatcacacca tgcttgaagt
      241 tatgcgggaa gctttggccc agtcgaatct gacggccaat gaaatttcag cgatcgggat
      301 tactaaccag cgtgaaacgg ctgtcgtctg ggataagaag accggcgacc cggtgtacaa
      361 tgccatcgtt tggcagtccc gtcagacggc ggaaatctgt gaagacttga aagctcgcgg
      421 cttggtcgat gaattccgtg aaaaaacggg cctggtcatc gatgcttatt tctcaggcac
      481 caaggtaaaa tggattctcg acaatgttga aggggccaga gaaaaggcgg aaaagggcga
      541 tctcctcttt gggaccatcg actgctggct tgtttggaag ttgaccggcg gcaaagttca
      601 cattacggac tattccaacg cgtcgcgtac acttatgttc aacattaaag aactaaagtg
      661 ggatgaccag ctccttgaat atttaaccgt tccgaaatcg atgctgccgg aagttcgccc
      721 gtcgagcgat atttatgggt ggacagaacc gtcgatcctc ggcggtatgg tcaatattgc
      781 cggcattgcc ggtgaccagc aggcagctct cttcggccag acctgttttg aaccgggcat
      841 ggccaagaat acctatggga cgggctgctt tatgttgatg aatacgggga cgacgcccat
      901 tccttctaaa aacggcttgg ttacgaccat tgcctggggc cttgacggca aagtcgaata
      961 tgccctcgaa ggttctatct tcgtcgccgg atcggccatt cagtggcttc gtgacgggct
     1021 gcggctcatc gatacggctc ccgattcgga atgggttgct aaacgggttc ccgattcgga
     1081 tggcgtctat gttgttccgg ccttcgtcgg ccttggcgct ccgtactggg atatggatgc
     1141 tcgtggtctc attatcggca ttacgcgggg cacgaagaag tctcacatcg tccgggcaac
     1201 cctcgattcc atggcttatc agacgaaaga tgtcctcggc gccatggaag ccgattcggg
     1261 tattaaattg gccgctttaa aggtagatgg cggcgccgtt gccaacaata tgctcatgca
     1321 gttccaagct gatatcctcg gcgttcctgt cgatcgacct caggtcattg aaacgacggc
     1381 tctcggtgcg gcctacttag caggtcttgc tgtcggcgtc tgggattcga aagaagatct
     1441 ggtctcgagc tggaaacttc agaatcgttt tgaaccgacg atggatcctg aaaaatcaag
     1501 tgcctattat aaaggctggc gcaaagcggt caaacacgca atgcattggc tgagtgatga
     1561 agactgacag agaggtgcat agctatgata tacgataact tacttggtga attcatcgga
     1621 acgatggtcc tcatcgtctt cgggggcggt gtctgtgcct gcaatacctt ggcaaaatcg
     1681 aaaggccagg gcggcggctg gatctgtatt acgacggcat ggggctttgc cgtaatgctc
     1741 ggcgtcttta cggcgacgac cttgggagca ccgcagggtg acctgaatcc tgccgtcacc
     1801 ttagggaaaa ccatgatcgg catctattcg gtaccgcagt ttattgttac ctctatcgtt
     1861 cagattctgg gcggcagcct cggtgcggct atcgtttggc tcgcttactt gccccattgg
     1921 gctgaaacgg aagacaaggc tgcgaagctc ggcattttct gtacggcccc ggctatccgc
     1981 aactatccgg ccaacttcct ttgcgaattt atcgcgtcct tcttcttgat gttcgtcatt
     2041 tggatgattt tttccccggc tgtcggcaag attcctgccg gcctcgggcc ctatatcgta
     2101 ggtgtactca tctgggcttt gggtatgagc cttggcggtc ctacagggta tgccatgaat
     2161 cccgcccgtg atctcggtcc tcgtctggct cacgctatcc tgccgattgc cggtaagggc
     2221 ggttccgatt gggggtatgc ctgggttcct gtcgtcgctc cgatggtcgg tgccttcgct
     2281 gcctacgtcg tcgctgcagc actgaacgta ttctaatttc atgatggtta tttcggttat
     2341 agggactcgg tacgggtccc tataatttta ggaatttaca aaatttctta tagaaatgaa
     2401 cttaatttca aacaggagaa atgtatatat catagcttat aatt
//
LOCUS       DAWDXZ010000265         2444 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8400_length_2444_cov_7.429050, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000265 DAWDXZ010000000
VERSION     DAWDXZ010000265.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8400_length_2444_cov_7.429050"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          604 a          668 c          475 g          697 t
ORIGIN      
        1 tctgagcctc gatcttgtcg ttctgtgacg ccagtttgct gttctgttcg tctacaaccg
       61 accgcaagtt gtcaatttcc tgagccatag cgcctttcga cgggtatttt cccgttccgc
      121 cttccccaaa tctccaagtt acgccggcat ttaccatatt ttcaccgccg ccgtaagacg
      181 acccgatact taccatggtg ttatagttcg ggcgatagaa ggcaccgaca gcaacggcgt
      241 tcgccccgcg atagttgcca atgcccgccg aaaattccca gcgtgacgtc ggatcaaaat
      301 ctaagggatg taaggctgcc aaagctgctg caccggcacc gacacgatcg acgcggttgt
      361 cgagtttgtt aatctgattt cccagttgat tcatacccaa cccgatattg ttaatcgtgg
      421 tgtcaaaagt tcccttgttg atggcatccg tagcttccgt agcttcgccg atgttgtgaa
      481 tagctttacc accggcgtcg ataccgtcct tcgtgacgga cggaccgtcg gtaatcgtca
      541 ggccgtcact actaattgtc gtatcacccg ttgtaacaga accggtcgta accgtattcc
      601 cgctgatgac cgtagtcgca tcaccagagc caacggtaat gctgtcgctg gcctttaagg
      661 tcttagtaga tacactgtcg agtccgttaa gtttcgaaga caattggact ttaaagcctt
      721 tgccattatt aacgatacca atatttttat ccgtcaagga attggtatca gcaccaccgg
      781 taagactcaa cgtatcggtt gtcccgattt caaccttagt tccactgtca ccgacaaagg
      841 tctgtttcgt atttttaatg ctgtcattga tcttaccgat ttccgtgcca ataatatctt
      901 ttaattgccc ttccgtagca gcacgatcgg caacgatatt atctgcatcc cacgacgtat
      961 tgctaagacc tgtaatgtat ttaccacttt ttttaacgac atcaccgttt tcatcaagct
     1021 tgtcagttcc atcggcattc ttaaggataa actcctgatt ccccattacg ataccagcat
     1081 ccgcagtacc aattacaacc tggccctttg cgccgtcaac tttcacttga ttatttgcat
     1141 tcgtgtcact gcctaaagta atcgtatctg cgaggctaat tttattcgta tctccatctt
     1201 tatttatagt aatattgttg ttccccgcta ccagcgatac tgcttttatg tcggcagtca
     1261 atttcgtatt taacgctctg agctgtgcta cgttaacggc atccatgttg tacgtgccgg
     1321 ccgctacctg tgtaatctga cgggtcttgc ccttgtcacc gacagagaca gccccaaggc
     1381 ctgacttcca agaaccggat gcatccgagc ttctatcctt cccttccgga tcatatccgg
     1441 caagaccggc tcgttgtttt gctacagagt aagaacctat agcgacgcca cctactccac
     1501 tatcttttgt tacactcgct ttatagccaa tcgcaatacc attttcttta ttagtgactt
     1561 ttgcagaagt ccctagcaca acagcatatt ccgatgtagt gacttctgct aaattaccga
     1621 ttacattaga gccggtagag tcagcaccaa cataggcttt gtcaccgatg gcaaccgaat
     1681 aggtactatc gaccttctta tagccgctgt ctgatttact tggatcgatt cgtacgccgg
     1741 taccgatagc cacggagcct tccaaagcag ctttagcatt atagccaata gcaacggaac
     1801 tcaaaccggc atcagattcg ctgccgccag caaatccaaa ctccccggaa tgagatttcc
     1861 aacccacggc caccccctgt tgtttatcta ccacactatt ctgaccgata gccagcgacg
     1921 ctgagccggt ggcattagca tcgttatagg gccatccacc cggctgcatg tcgataaact
     1981 taagcccaat ctgtaccgcc tttccagttg aacttttagt taatttaata cctcgtccag
     2041 cctgtagaac aaccgtatca ccgggttcaa tttctgtagt agaacccgtt gccgtgcctt
     2101 catgttcggt ttttacatca gggcctacac ttacaccaaa tttccaaatt gtatccgtat
     2161 cggcagccat gacaccgggc atcatcccgg cacaaagagc cattacggca agggagacag
     2221 ccattttttt actcatccga gaggatgacg atacagcctt accatcggtt cttcctaatt
     2281 cagacacgac catacagcaa tgacgaactt tactccaaat acctttataa atctgattca
     2341 tgcttttcct cctgtatcct ttaattaaaa aaatataaaa agaaatgtat gaaaaaaatt
     2401 tagaagctat ctatcctaaa agatccgtac tacctcgatt gatc
//
LOCUS       DAWDXZ010000266         2434 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8438_length_2434_cov_5.912568, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000266 DAWDXZ010000000
VERSION     DAWDXZ010000266.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8438_length_2434_cov_5.912568"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    675..684
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1894..1903
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          503 a          576 c          730 g          605 t
ORIGIN      
        1 aatgaaatgg cttccattta tgatagtgat tgaacagctt atcgttttgc cgtataatga
       61 aattataatc agtattttta ggcccatcga gccgggagga gtgacctgtc tatggattat
      121 aaggggaaaa aatgtctcat tgcttatttt tcacatgccg gtatgaatta tgtcagcggt
      181 aagattgtca atcttaaggt tggtaatacg gagcaggcgg ctaagatgat cggccgcatg
      241 accggcgggg acctctttca tatccgggca gcagccgtct atcctcatga gtataaagaa
      301 acggtctccc tggcccgcca tgaatgggaa aagaggcttc ggccggccct gtcaaaggat
      361 cttgacgata tcagcggtta cgatctcatc tttctcggct atcccaattg gtgcggaacc
      421 atgcctatgg ccctgtggac tttcttagaa aatcacgatt tttccggcaa aaccatctat
      481 cctttctgca cccatgaagg gagtggtatc ggccgcagtc tggaagacat ccatgaactt
      541 tgccctaagg ccgtgctggg gtcgcacctg gctattttag gttcgaccgt tcaggacgcc
      601 gaagacgatg tgcgccgctg gctgaacgaa taagtttttg catataaaaa gcactgttcc
      661 tcggaacagt gcttnnnnnn nnnnttttta ttcatagcgc aaggcttcga tcggatcgag
      721 cttggcggct tttcgcgccg gatagatgcc gaagaagagg ccgatgccga cggagaagct
      781 gaaggaaata ataatcggtg ctgccgtaat gaccgtctca aagaggcccg actcggcgat
      841 ggctaaggcg atgccgacgc cgcaggcgac gcccaagagg ccgccgatga cgccgatgac
      901 gacggactcg atgagaaact gcatcatgat gttctggaag gtagctccca gggctttgcg
      961 aatgccgatt tcacgggttc tttcggtaac agataccatc atgatgttca tgatgccgat
     1021 cccgccgacg acgagggaga tggcggcgat actgcctaag aacaaggtca gcatggtcgt
     1081 cgtctgggtc atcgtttcca tgaggctggt caggttgcgg acgttgaagt catcttcctt
     1141 gccgttggtg atgtgatgac gctggcgaag gagggcttcg acttccgatt ggacctgttc
     1201 gattttatcg gcactggaga cctggatgtt gatggacttg atgtaggtaa tgcccagcaa
     1261 tcgttcttgg gccgtcgtca agggtacgat gaccatgtcg tcctggtcct ggccgacgga
     1321 agactggccc ttgctttcga ggacgccgat gactttgtac ggctggttat tgatgcggat
     1381 cgtcttgccg acggggttgg tttcatcaaa gaggttggtg gcgaccgtcg ttccgatgac
     1441 ggcaacgcgg ttgcgggaat ttaaatcgga ctgggtgatg aaagaacctg attggacagt
     1501 gagggaccgg atgtgcaggt attccggcgt aacgccgtag accgacgtgt tccagttctg
     1561 gttgccgttg acgacttggt atgagctgtt gaccgtcggc gacacgtagt cgatgttctt
     1621 aatctgcttc ttgatggcta cggcatcgtc gtatttcaac tttttattgg agccggctgc
     1681 cgagcggacg ccgccgctgt tagccgatcc cggcatgacg atgagcatgt tactgccgag
     1741 gctggcaatg gagttggtga cgttttggcg gacgcccatg ccgatggaaa tcatggcgat
     1801 gacggcgccg acaccgatga tgatgcccag catggtcagt atggaacgca gcttgtttga
     1861 taagagcgcc gtgatggcaa tttggacgct ttcnnnnnnn nnnacatcca tgacgacccc
     1921 ctttccggca tctttggtaa tcttaccgtc tgacaagagg agctgacggg tcgcacaagc
     1981 ggcgatttcc ggttcgtggg tgaccaagat gatcgtcttg tggtcttcgt ggagctcttc
     2041 aaagaggcgc atgatatctt tggtcgactt cgtatcgagg ttgcccgtcg gttcgtcggc
     2101 catgatgatg tgcgggttgt tgaccaaggc tctggcgatg gccactcgct ggcgctggcc
     2161 gccggagagt tcattcggct tgtgatgggc ccacgtttca aggccgacgg atttcagttt
     2221 atccatggcc cgttcggtcc gctctttgcg gccgacaccg gcatagacca ggggaagggc
     2281 gacgttgtcg agagctgtaa ttcgcgacag gaggttgaag ttctggaaga cgaagccgat
     2341 gtgctggttg cggacgacgg cgagttcatc gtcgctcagg tgggcaactt cctttcctcc
     2401 cagctggtac gagccggctg tcggcctgtc gagg
//
LOCUS       DAWDXZ010000267         2430 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8452_length_2430_cov_6.670316, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000267 DAWDXZ010000000
VERSION     DAWDXZ010000267.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8452_length_2430_cov_6.670316"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          608 a          669 c          672 g          481 t
ORIGIN      
        1 tcgaagccgg cgaactgtac gatgaagctc gtgaatcggt ccgccgcttc atcaacgctc
       61 cgtcgacgga agaaatcgtc ttcgtccgca atacgacgga agccttgaac ctcatcgccc
      121 gcagctacgc tgaaccgaaa ctgaaaaagg gcgacaagat cgtcatcccc atttccgaac
      181 accattccaa cctggtcacc tggcagcggg tctgcgaaaa gaccggtgcc gtcctggaat
      241 tcatgtacct cgataaagaa ggccacttca cggatgaaga tttggccaag atcgacgaca
      301 agaccaagat cgtttccttc gcggccgtca gtaacgtctt cggcatgaag cgtcccgtca
      361 aggatatcgt cgccaaggct cattccgtcg gtgccgttgc catcgtcgac ggcgctcagt
      421 ccgtaccgca catgaagacc gacgtccagg atctcgactg cgacttcttc gtcttctccg
      481 gccacaagat gtgcggctcg gccagcacgg gcgtcctcta cgggaaaaag gccatcctcg
      541 aagggatgga acccttcctc ctcggcggcg acatgatcga atacgtcaag gaacagtcga
      601 cgaccttcaa cgaactgccc ttcaaattcg aagccggctc gcagaacgtc gaaggcgctg
      661 tggccctcca cgctgccatc gactacttgg aaaacctcgg catggataag gtcgaagccc
      721 acgaagaagc cttgaccaaa cgctgcctgg aagggatgga aaagattcct cacatccaca
      781 tcatcggcag caccgatccg acggaaaaga ccggtgtcat caccttcacc atcgacggcg
      841 tccatcctca tgacgcagcg accatcctcg acagcttcgg catcgccatc cgttcgggtc
      901 accactgcgc ccagcctctc ggcgctcatt taggcgtaga agcgtcgaac cgggccagct
      961 tctatattta caatacggaa gaagaagtcg actacttcct ggaaaaactg cctctcgtcc
     1021 gcaagcagat gggttttaaa gactaaggag tgttcaacga gttatggaaa atatgaacga
     1081 attgtattcc gacatcattc tggaacacaa tcaatcgcaa gccaataaac gcctcttgga
     1141 cgttgccaac atcagtgaac acggccacaa tccgagctgc ggcgacgata ttacccttca
     1201 ggccgacatt caggacggcg tcgtcaaaga tgctgcctat acgggccacg gctgtgccat
     1261 cagccaggct tcggcggata tcatgatcga cctcatcaaa ggtaagagcg tcgaagaagc
     1321 ccttcgtctg gtcgatctct tcctggacat gatcaagcgg gaaatcaccg acgatgatga
     1381 gctggaagaa ttggaagacg ccatcgcctt gaagaacatt tccaacatgc cggcccgcgt
     1441 caaatgcgcc gtcttggcat ggcatacctt aaaggctgcc ttggaaaaag gccaaaacta
     1501 aatcttccta aaaacaaagt gcctctgtag ggcatgataa aaggacagtc ccagacgggg
     1561 ctgtcctttt tgtatcgcta agatgatatc cttgtgttac gcctgagctt tatcttccgt
     1621 gtcggttgtc cctttgaact gcaggtagac gaagacgacg aaggcgatgg cgaaggcgat
     1681 gacgctggtc atttgtgccg atttcaggcc cagggtcagg tcgacgtagt cgccgcggag
     1741 aaattccaag aagaaccgtt cgatggcgta gagcatgacg tagaggctga atacctggcc
     1801 cttcttgtgg gggaaggagc tgtaaaagag gagggcgacg aagaccagga cgtcgatctg
     1861 tccttcccag acttcggccg gccataaggg ctggctgctg taggtctgat aggcgagggt
     1921 cgattcggga tagacgatgc cgaagttgcc ccccgtcgga tggccgaagg cgtcgccgtt
     1981 catgaggttg gccatgcggc cgatggactg gcccaggatg atggccggtg ccaacaggtc
     2041 gccgaaggcc caggtgtcga tatggtagtg ttttgtatag aggtagccgg caatggcgcc
     2101 gaataagagg ccgccctgga tggccatgcc cccttgccag acgtaaggga tttccgtcag
     2161 gtgatgctgg tagtaggccc agtcgaagaa gaagacgtcc cacaggcggc cgccgacgat
     2221 gcccgccagg ccgcagtaca tggtgaagtc gaagatgtgg acgtgccagc cgcggccgtc
     2281 gcgcttcatg atgtagtacg ccgtaagacc cgacgagatg atgcccagca taaataacag
     2341 tccgtaagcc cggacgggga aggagccgat gaaaaataga tactgatgca atataaaacc
     2401 tcctttgggt gtggtataat gaaaaaatat
//
LOCUS       DAWDXZ010000268         2428 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8461_length_2428_cov_8.752212, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000268 DAWDXZ010000000
VERSION     DAWDXZ010000268.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8461_length_2428_cov_8.752212"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    376..385
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    769..778
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          662 a          590 c          646 g          510 t
ORIGIN      
        1 gaggttgttc ttgaggtccc caaaaaggac ctgcacgacg acgagatagc gcgtatcaac
       61 gctatcatgt gcgcacccat tccatgggcg ccgggactca tcctcaatgc agacggcttc
      121 atcagtccgt actacaaaaa agattgacga ccataagagg tacactatga cggcacaaga
      181 acttaataag tatcgcaaga aaagacatta tatcttaaag aaaagctggc aaaaatctat
      241 ccggaccacc aaaaccggaa gccgatgccg gcacgctaag agtctagtac gcagtggccg
      301 ccgatacaag ggcggccggt aaaggaggaa aactatcatg ttcgagagat tatccagttt
      361 tttagttgtc attatnnnnn nnnnngcatt tggctacccg aattaccttg tatggcagca
      421 gggcaaacag ggcgaagcgg ccctggctaa ggcgactcag gaccgccaaa tcaaggtcca
      481 ggaagcggag gccgaattag aagccgccaa aaagcaagcc gaagctaacc ggattctggg
      541 cgagtccatc cgggcttacc cggaatcgtt agaacagaaa tgggtagagg ctgtacggga
      601 taccaagaat caagtcatct acttaccgac agaagcgtca gtaccgatta cggaggccgg
      661 ccgaatggcc gcaaaagctc agcatcagga ggaaaaataa tatgaaaaaa gtcatcaacc
      721 ttacaccaca tgtagtaaat gtctacgacc ttagcaataa tcctgtatnn nnnnnnnnat
      781 cccggtatcg gggatgccca ttccgcgctg ccatcaagat caggtagtgg taggaatcat
      841 cgataatatc acgattacgc gccaatcttt cggcgatgta gaaaacctgc ctgcgccagt
      901 gcccgacgta tactatgttg tatcgcgcat ggttgccgat gcaatgccgg aacgcggtga
      961 tctgctcgtt ccagggcctc ttgttcgcga ttcccagggg aatccatgcg ggtgccgggg
     1021 cctggctgtt gtgggcccgt ggaaacgctc ggctacggcg accttcggaa aatataaacc
     1081 tgttgaagca tatgttgctg aattaaccga catcgaaact tataaattcg ccttccggaa
     1141 tggttatggc gccagcgtca tccgggggcc gcggacctat ggcggcgaag aaggtacatg
     1201 ggaattagcc gtattaaaac aaggaagcat atgcgataaa acgcctatca ccgacgatgt
     1261 tattggcggg atcactgatg atgaggtaat tgagttatta cagcgcatcg aggcgttgta
     1321 atgcttaaaa ttctggagct tttcgggggc atcggcagcc cccggattgc actccgaaac
     1381 atcggcgtac cggttaagag tatcgactat gtcgaggtcg acgagaaggc cgtcaggtcg
     1441 tacaatgcga tgttctgcga cgacctagcc tatacctcgc aagacgtgcg aggttggaac
     1501 ctgaaaccgg acatcctcat acacggctca ccgtgtcagg atttttccat cgccggacgc
     1561 cagcaaggcg cggaccccgg cagcgagaca aggtcatcgc tgatgtggga gaccatcaac
     1621 atcgtccgca acatgggagt ttggcggccg cgcattatca tctgggaaaa tgtccggaac
     1681 gtcatctccc ggcacatgat acgtaactat caacggtacg tgctcgagct taataagcta
     1741 gggtacacca gtagctacga gatcctcaac gcaatggact tcgggctgcc gcaaaaacgc
     1801 gaacgagtgt ttacggtctc gacgctaagc ggccgggagt tcgacttttc aaaaatgcac
     1861 cggcgacgga tgcgacctat aagcgatttt ttggaatctg ggcccgttga tgagtactac
     1921 accatcacgg cgccttccat gctggcggcc attggcaaga caggctgcat cagacgtatc
     1981 ccaatcattg aggactattg ttacaccatt accgagcgcc ccgatcgggc gccaggtagt
     2041 ggatgcctgc cgataggcgg aggtaagtat cggtacttga ccgagcggga atgttggcgg
     2101 ctgcaaggat acagcgatga tgactttgag gcggcagctg ccgtcaatac caggcggaca
     2161 ttataccgcc aagccgggaa cagcatcccg gtaatgattt ttgaaagtat gttcggcgaa
     2221 atgctgaaat tgtgaaaagg agactaaagc atgaaaaagc ttaaatacct gtacagatac
     2281 agtgctaaat caatcggaaa tgcgctctat tggagactct acggctggga tatcgtcgaa
     2341 aaggatcacg gctatcgatg tgtaacaatc ctccgagcct ataaactgtc caattattac
     2401 ttatctaagc attcgaggat tgagcatt
//
LOCUS       DAWDXZ010000269         2427 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8466_length_2427_cov_6.874789, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000269 DAWDXZ010000000
VERSION     DAWDXZ010000269.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8466_length_2427_cov_6.874789"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    315..324
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          536 a          580 c          592 g          709 t
ORIGIN      
        1 cgatgttgcg cttcgggacg ttgatgatac gggtcaggct caggctgtct tcgggattat
       61 acaagagccg caggtaagcg aggacgtcct tgatttcctt gcggtcggag accttcgtgc
      121 cgccgaccat ggtataagga atggcgtagc gcatcaattt ttcttcgagg acacgggact
      181 gggcgttggt ccggatgagg atggccatgt cgccgtaggg ttcgtggctg atttcatggc
      241 gattgtagat ggcgccggcg atgtagtcgg cttcgtcgtg ttcggtctgg gcctggtaat
      301 ggatgacctt gttgnnnnnn nnnncgtggt tcgtatcctg atattcgtcg accaggacgt
      361 attggaaccg ttcctgatac ttttcccgga cgtcgtcttt ttcttccagg aggcggacgg
      421 ccaagaacag gaggtcgtcg aagtcaagag cgttgttttc cttgagcttt gcctgataga
      481 gcgcataggc ttcggagact ttttgctggt aaaagtcttc ggcctggccg gcaaaggttt
      541 tggcatccat gaggacgttc ttagccgccg aaatcgtccc caagaccgac cgcggcgtga
      601 attgtttatc gtcgaggttc aattttttca ggcagtcctt gatgagcacc agttggtccg
      661 acgaatcgta aatggtgaaa ttccgggtgt agccgtgaaa gccgtcgatt tcaaagcgca
      721 gcagtttcgc acagaaggaa tggaaggtgc tgagccaaat ggaatcggcc tgggccccga
      781 cgaggttctg gacgcgctct ttcatttcct tggcggcctt attggtaaag gtaatggcca
      841 agatgcggta gggcgagacg ccgagctcca agaggtgggc gatgcggcag gtcaaaacct
      901 tggtcttccc cgatccggcg ccggcggtaa tgagaagctg ctacttaaaa catagataaa
      961 cgtatctata ttaagatgaa ttgctgcaac ctcgcggttc gcagttcctt cttcatcgta
     1021 cactcttttg ctctgagcaa gctcagcagc tacttgaggg gttctgcact ccaaaggcgt
     1081 tgacaacctg cttcttgcgc agcaaggaga aagatgtctg ttcccgacta gccatcggta
     1141 catatatcag tacttttgga tttaagcggt ctgatattct tttgcatccc gcagattaag
     1201 cgaggcgttg agatcacggt caatcatata tccacacttt gggcagacat acatgcggtc
     1261 ttggagtttc agccctttat ggatataccc acaggcatgg caagttttgg agctgggata
     1321 gaaccggtcc acgacccgca attcgatgcc tatgatttct gccttgcatt tcagtttcga
     1381 caggaaagaa tagaaccgtt ctgctgcaac tgattttgcg agatgcttgt tcttcatcat
     1441 gcctttgata ttcaagtcct ccacggtaac gaagcttggt ttttgcttca caatctcatg
     1501 aatggtctta ttttcatgat cctcacggat ttgtttaatt cgctgatgaa gtttctgcac
     1561 cttaagtttt tgcttgtcta tgtttgcaga ggctatagca ggtgcaccac ctttcttttt
     1621 tttaagctca tatttccggc tgagctgcct ctgcattctt ctcagccgct tctccagtcg
     1681 tctgaccttt gaagatttgt tgatattttt aaaggtctgg ccattgctga caatcgccaa
     1741 attcttgata cctaaatcga ttcctatacc ttcgctctgc ggatgacagg atgccgccca
     1801 atcgttgttg tatctggatg tctcctcaac atctgccaca acggaaacat agaatcggcc
     1861 tgcctgataa gaaactgtgc cgcttatgac ggcgacacca acggggagat atccgtattc
     1921 tttcagcttc acctgtttca gtgtcggtat catcagcttg tgccgccata tcttccagtc
     1981 acccttgtta ttcctcggaa aatacagctt cacatcctgt cgggatttct ttttaaaacg
     2041 cggaaatcct gcctgccctt taaaaaactc tttgaaagcc gcttcagcat taaccagcgc
     2101 tttcttgcgg gcttttgccc cgcactcgct tatccaagac agttcctgtt tcagtttgtg
     2161 attgacgtac ttgtcgaagt cattagccga tacaaaatgc ttctgatgac tatcaagcaa
     2221 cccacgctga tacatccggt aaagcttctt attataggca atgtactgat tatataaaaa
     2281 acgacagata ccgattgagc gaataatcct attggcttgt tcgagtgtcg gagcaatctc
     2341 cgtcttgaac gcttttagca atcgtctcat ctccctcgat ttacttctta tattttctca
     2401 tgcagtccat atatactgca tgagaat
//
LOCUS       DAWDXZ010000270         2425 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8468_length_2425_cov_9.904641, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000270 DAWDXZ010000000
VERSION     DAWDXZ010000270.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8468_length_2425_cov_9.904641"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1703..1712
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2166..2175
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          576 a          616 c          569 g          644 t
ORIGIN      
        1 ggctcggcat cgtccttgaa aaacttcgat acggaggaaa acttgccggt gcattcaaag
       61 actctgacca tggtccgcag cctcgtttcc tgtcgcttcc aatcggccgg tctcaatcgc
      121 tcttgccaat actgtttccg ggacacgata cggtatccct tgaaggtaag tctgcgatat
      181 tccttccgtg gaaactcaag atcttttttc aaggaagctt ttcccttttc cttcgaagga
      241 actttaccgg cttctttggt cggcatttcg ccatctgttt tcaaggaagc ctttcttttt
      301 ctttgccatc tctctaccgt aagggcattc aaaaagtact gctgccacgt ttggtaattc
      361 gccttttgcc gattacagtc ggcgatgata gaatctttga ccggataaaa gatacgccgg
      421 cccacgattt cacggggaat ccattcgctg cacagggtca tggcttgttg gaggtagcct
      481 ttttcgacgc accagcggat gatatcgaag ttggtaaaat cttctttcaa gagggacccg
      541 tactcccatt tgaagacttc caagatgcgc agaaagattt tttcctgcag cgaaacagcg
      601 ccggcctgtt caaagttacg caaggcttct tgaagtttct tggccaaggg cgcaattttc
      661 ccggtccggc agatgcggac ggcattggta aaatcgcgca tcgtctccaa gagcagcttc
      721 gtttccgccg tctgcttcgc cccttccata taagccatga tttcgcgggt gctgccgaaa
      781 ttgatgaact cgtcggctcc ggaaatgagg ttgaacatgc ggtagatatc gttgacattt
      841 tcgacctgct cttcatagcg gttcgaataa aggacgccca tcgtccggat gccgcggtat
      901 ttcaagagct gcatgaccga cagcatcatc atcgaggcat gacgaaaacc accggtaaaa
      961 tcggcatgga gggccacctc ttgcggctgc catgacagtt cctccataaa ggtccgaatg
     1021 gtgtcggcca tctggaggac ctgtcgaata ctttcctcgg tctgactccg ttcatcgtaa
     1081 tcgatgcgga tacagtgcgg cacaaaggat cgtacccgct cgaggaagag gtcctcatgg
     1141 gtcatcgtac gctcatcgcc gacgtcgtcg atatagcata tttcttcctg cgtccgcttc
     1201 gttgaaaagt agaacaggca gtccaggcga tccccctgct tttgtaatac atcggcagtc
     1261 cggcgaacgg ccgattcatt cgtttgaaca cagtatatca tcgtcccgtc gagattctta
     1321 taacgagaaa cggataaggt gcggtcgcgg ttgagatgga cttcgcttaa aaacagcagc
     1381 atgatatgct tcatagatgg attcctcttt tcggcagcca tggtcgcgct cgcccatgac
     1441 gatgatggaa ggccatatcg gcggcgcctc tgtgaggccc ttcgtcagac gaagcagact
     1501 ttcccgttct atggcttaaa ctcaatagaa tacatgtgat gccccggcgt atgcctttct
     1561 tctctactac ccggaacttc ggcctcgatt gccatcggtc ccttcggcgg tccgaaggtc
     1621 aacacctttt cagccaacag gcgttttcga aaggattcaa aaaaggaccg gacccgcatg
     1681 tccttcacgc ggccaaagag gcnnnnnnnn nntcctcaag ccgcggttcg tcgtatacat
     1741 agataagatc gattcgatgg tcatagatat gggccaccgt ctttttatcg ccggaggtga
     1801 cttcaatccg attcgcttta tcgacgtagg tgattttagg acggggaaat atctcgcagg
     1861 cctctttaaa ggacgcattc gctgccaagt cttttaaatt ggtaatcgca caatcggaga
     1921 aattaaaggt atatcgctct tctatcagga taatatcatt cgtaaaggac gttcccatgg
     1981 cctttcgctc ccttctgaag ttaactaaag acacgacaac ctccggacat aggaagcggc
     2041 ccttacatcc ggaggtcttt tcagtccccc tatgtgataa actccttttt tcaaaaagga
     2101 gtccaattat ttccggggaa tcgggtaata caatgcagta gccatagcga gaatcctgag
     2161 gtaatnnnnn nnnnnaggta atcaccgtat ccgtcccctt acgcggagtc aggaaatgcg
     2221 gccgtcaacg tattaccctt attaatattc agtatacgtt ttccaccatc tcttgtcaaa
     2281 taggtcacat cccgtctcaa tcagacagtt aagccgattg ttccggtgct ttttggacaa
     2341 aatcagtggt cacgcaattc caagtgtata taattttgtg atataatggt gatatgaata
     2401 acgaaggagt gaatcttaca tggca
//
LOCUS       DAWDXZ010000271         2424 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8471_length_2424_cov_9.215703, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000271 DAWDXZ010000000
VERSION     DAWDXZ010000271.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8471_length_2424_cov_9.215703"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1569..1578
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          551 a          666 c          688 g          509 t
ORIGIN      
        1 tatctcgtcg acgaaccgcg gcggggacgg gccggccgtt ctaccgtccc gacttcaatc
       61 agcgcccgtc cggacgacca actttggttc aacaacttcc gcatcggcca gtggcctgat
      121 tataacgaca acgtagcctt ccccaaagat gccgcttcac aggaccagtt tttccgggct
      181 atgacaccgg acacgggggc ttttgacggc catgtcgtcg ccaccgccat gacggctgtc
      241 ttcgataagg tcggcgacgg cgtcttgatg actcattcgg ccggcggcgg tcccggctgg
      301 gaaacggcca tggcctcgtc ccacgtaaaa ggcgtcatcg ctttggaacc gggcaccttc
      361 cccttcccgg aagggatggt tccgcctgtc gaagaaacga ccagcccatt cccgactaag
      421 gggacggccg tttccatgga tgatttcatg aaactgacta agattcccat cgtcgtctat
      481 tatggagaca acatcccgaa aggcgataca ccggtggcca attggggcga agacacttgg
      541 cgcatccgct ggaatctggc caagaaatgg gaagccctgg tcaatcagta cggcggccat
      601 gtggaagtcg tcagcctgcc cgatatcggc atcaagggca acacccattt catgatgtcc
      661 gacctcaata acgaggaagt ggccgacgcc atcgcagcct ggctccataa acaaggttta
      721 gataaataat tttattatcg gacaaggggg attttggtat gtggaaaaaa atcatgagcg
      781 gtttattgat ggctgtcgcc ttagtgaccc ttacggcctg cggcagtacg gggacgacgg
      841 cggcacagcc ggcatcggac ggggctaagg ctgccgcttc tcaaggaaag gtcttagtcg
      901 tctacttctc ggcaacaggc aatacgaaac gcgtggccga aacgattgcc aaccatgaaa
      961 atgcgacggt ctttgaaatc aagccgaccg agccatattc cgacagcgac ctcaactacc
     1021 gcgacgacaa cagccgcgtc gttcgcgaac acgcccagcc tcagctgcgg ccgacctatg
     1081 aaggcgacgt cgccgactgg gattcctacg atacggtcta catcggctac cccctgtggt
     1141 ggcagcaggc gccgcatgtc gtctacacct tcgtggaagg tcataacttc agcggcaaaa
     1201 aggtcattcc tttctgcacg tcagcggcaa gctccctcgg ggacagcggt cagaacctgg
     1261 ccaaggcagc aggcaccggc gattggcaga ccggtgtccg cttcaatggc ggggccagcg
     1321 acaacgacgt cttgaactgg ctgcagcagc agtaagaaaa aatccggcag gaataaatcg
     1381 attccttagg gaaaagataa taaaaaaaga gattttttgt tccgagcaac ctcgacgggc
     1441 tgctcggaat ttttttgctg ccaaaacata gaatctcagg cttttcgttg gtggaaagga
     1501 tgtgccggga aagagggggt gcgataccga tatcggtaca caccatacac cgatatcggt
     1561 ataaatatnn nnnnnnnnta atatctttgg tttatattaa cggattgcag aggcaccgat
     1621 ttttttagaa gggggacagg tcgtgaacgc agttgttttt tcctttacca aagccggaac
     1681 ggcccttggc ggtagagcag cagccttcct tcagcaaaat ggctggcagg tccgccgggc
     1741 gacgctcgcg aagtttgcca taccgccggc cgtctccttc gacccgtctc tgcgggaaga
     1801 aacgggccgg gcttttcaat cggcacggct cctccttttc gtcggggcag cggccatcgc
     1861 cgtgcggtct attgcgtctt ttatccgtaa aaaggatcag gacccggcgg ttatcgtcat
     1921 cgacgaaaaa gggcagttcg tcattcccat cttgtccggt catattggca gagccaatga
     1981 atgggcccgg caagtggctg aggccatagg gggacaggcc gtcattacga cggcaaccga
     2041 cgtcaatgcc ttatttgccg tcgatgaatg ggccgcaaag gccggtctgc gcctgtcttc
     2101 tctgacggag gccaaggcct ttgctgcggg tcttctggaa aggggaaggg ctgagtttcc
     2161 cattgaaggt cctttgccgc cgggcttggt catgacgacg gattgtgaaa cggggttgat
     2221 tgtgacgacg aaagagaacc tgtccctttt tcaaacttcc gtcatggtaa gaccggctat
     2281 tctccatgtc ggcatcggct gccgccgcgg aacctcatcc tcggctattg aaaatgccgt
     2341 ctcatcggcc cttaaagagg ccaacctctc gaaagaggcc gtggcggacg tcagcagcct
     2401 cgacgtcaag cgggatgaaa cggg
//
LOCUS       DAWDXZ010000272         2423 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8478_length_2423_cov_9.210726, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000272 DAWDXZ010000000
VERSION     DAWDXZ010000272.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8478_length_2423_cov_9.210726"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1066..1075
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          616 a          685 c          565 g          547 t
ORIGIN      
        1 atgccgccga taagggtaat cccgatgaat acgacggcac tggtgaacat ccccgtaacc
       61 ggcgtgaaaa tatccggcat gagggctgcc aggacggcgc cgccggctac gtactgaaga
      121 ctcatgacga cgagctgtac caaaacctgg caggcaatgc cggccaccat gctgcggcgg
      181 tcataatacc gttcaaggag ttccggaacg gtcgtaatat tgagacggcg atacttcttg
      241 gcgaccgtca atcccatgac aatggcaccg atcccccagg ctgccgtata ccatccggcg
      301 gacaagccga ctttataagc ctgttcggca acgccgatcg tcgaggcacc gccgacggca
      361 aggccgacga tggataccat aataagaggc gtcgtaagtt tgcggccggc caatacatag
      421 gcttcggcag aatccgcagc ccgccgcttc acataccagc tgataccgaa aagtacgccg
      481 atataagcta aaataataac gatttgaatt cctgttgttc ccatcataaa aactcctctc
      541 ttcctaattt cccgaaaaaa aaatcgcccc taaaaaggga cgacgacgtc gcggtaccac
      601 ccaatttatc gatagaacaa aaaaagggtg gcacccaggg acggaaaaat ccgcggtacc
      661 accctattta tcttgaatcg atcaactcat ggccgtataa cggcagccgc cccggtacag
      721 cctactacag ttcagccgta cagttcagga atgaacttca gacttcatcg cctgtaccct
      781 cacaccaccc ggatactctc tgaaaagttt aaaggcctta cttttttccg tcacaactgt
      841 tatcaggaaa ttaattgttg ataatgatta taccggaaaa taaatgaaag tcaagagaat
      901 attttcataa aaaccgacga aaaccacgta aaacgtcgta ctaatccggt aatacctgcg
      961 atttcaagat taaaacgatt tctgtattct tatgcgactt ataatgatag cggaataatt
     1021 tcccgattaa aggcaggtct gccaaaatcg ggacttttcg aaaatnnnnn nnnnnctgac
     1081 ttcggcaaat aaatccgccg tcaccgaatt atccggatga acttgagggg tataagtcaa
     1141 tttaatgcct gcatcttcat aggtcgtact aatagccgtc tcctgtccgg accgatgctc
     1201 ggtctgaacg ggaatcttat cgccaatgag gatatgagct tctctgccat tgctggctac
     1261 aatatggggc cgggctaaga ttttcgcctt accgcgttct tctaaggcat gaatcgtccc
     1321 agacagagaa aaaatactgt gagcggcgcc gccttccacg gtcgaccaat tgtactcgat
     1381 accggtctgc ttcaacaggc tgtcatcgat ggaagcgatt tcaacagaca catcgacttg
     1441 ccgcggcggc acatcgagtc gcttgaccaa ggcctgtaca gccgcctgag tctgtgcggt
     1501 tccgccgacg accaccgtat tcgtatcgcc gtgccagcgt acgtcggctt cagggacggc
     1561 tgcctcaaca gcttccttta ccgcttccgg atcggcatag cggagccgcc atgtatgaaa
     1621 cgtttttaca cgctttccgg tatgaggacc ggtaatcgtc cgtatcggcc cggaggcatc
     1681 gtaatagaga cctttactgt ccgcaatcgc ctgcagggcc tcttcgacgg taatatcccg
     1741 cagggtcatc gtgagtgtgc cttctacact gtcatcgacg ataagattca tctgtcccga
     1801 ccgtgccagt ccttcgagaa cggtccgaac agggacatcc ctcacctgaa tatcgatggc
     1861 agccgcctga atgggcagga aacaacagac agctgccgcc caggctttgg tataaaactt
     1921 catgacactt cctttctccc cctcacaagc gaatctccca aagttttccg tcacgcataa
     1981 gccctacagc ttggcggttg atataagcaa tataccagcc ctgccaggat tctccggcag
     2041 cgcaggcagc cgactggccc tgccactgca acaggaccag gtgatccttc ccttgatgaa
     2101 tcgacccgcc aacggtgggc aaggtccatt gcggctgaac ggattccgtt gtctttaccg
     2161 atttcgcagt cggtgacata tcgggtcccc cactttgttc cgcggcgaca gctttcttcg
     2221 gcaaactgcc gccaaataaa tcaggcaggg gcttatactg tatcgccggc cttacgtcgt
     2281 agacgaggct tccttcagcc tccttctccg gcgtatcaaa aacgggatgc ttctcagctt
     2341 gctgctgaga ggcatcccgt ttcatgttta cttgtccctg gggctcttcc cacaaaaacc
     2401 aaacagcgcc aaggcagaca agt
//
LOCUS       DAWDXZ010000273         2416 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8503_length_2416_cov_6.321050, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000273 DAWDXZ010000000
VERSION     DAWDXZ010000273.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8503_length_2416_cov_6.321050"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1166..1196
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1702..1774
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2173..2182
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          493 a          486 c          642 g          681 t
ORIGIN      
        1 aattgtttgc ccgctgtcaa aatggtacaa gttccgtagg atacctcaag tcattgactt
       61 tgctattctt atcttcatag cttatcagta taaatggtat tatgcagcaa ttttacttat
      121 ccttgatttt gcaataacaa cattcatccc tgttccaaaa agcaagatgg attgggcaaa
      181 agataaaatg gatgaaacag cactacaact cgcgaagtaa gtgtcgatgt tctttatcca
      241 taagttcatg gattgaaaca tttaccttgt gcatttgttg tcggattgtt ataacgtcga
      301 tgaacgtcta ctgcaagtga taccaataaa taagggggat tatgacatac aaggaaactc
      361 atcagttatt atgtagatgt attgaaggat ataggaaggt gttattatga acgttctgcg
      421 ttggatactt gttttaccgt cttttattgt ttcttatggc cttgttcgtt ttattgccct
      481 agcactcatt ttaatctttg ccgggacgac gggctttctc tatgcatttt gttccatcgt
      541 tgttgaaggg atacttgcta ccatgtttgc actggccact tccgtttggg tagcaccgtc
      601 gcaccgtgac aaagtagtat atgcgttgtc ggcattcgtc gtaattgtat cagccattag
      661 cttgttcctg ggagttggaa aatatggttt tggaaatatg ccggactggc aaggagctat
      721 cacggttgca gcaatggccg ttacttgttt atatgtatcc tatcttacgc acaaggaggg
      781 gacggtaaaa gttttcggaa gataaaaagc ccacataata agtatcgtgt gagtataata
      841 ctatggtcat acccttcatg ggtgcgtgga ttgaaactac atgaacgatt ccctgtcaat
      901 tgcgatgccg agtcgcaccc tccatgggtg cgtggattga aactcgaata atttgctcat
      961 tcagctttat cggctcgttg cacccttcat gggcgcgtga atcgaaactg gcaaaaagaa
     1021 taccctctct acaccggtag ggagggtatt tctatgctcg aatcagtctt tgaagtaagg
     1081 cttaaacacg gaacgattcg gttctttatg ttgttttttt tcggagcgct ggttgttacg
     1141 gaaggtgccg tggttattgg agtcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntctt
     1201 tccgattgcg gtcgttgcgg cgatagttgc ggtcgcgatt atttttccct tggcggctgc
     1261 gatggacgcg gagaggggct tcttcggtaa tcttgacggg cgtcgtgtcc ggttctttcg
     1321 tgagcagctt gatagcggct gcaacgaggg acgccgagtc gtagtttgcc aggagttctt
     1381 cggcattttc tttatagctt ccgaagttgc cttcttctgc ggtagcggcc aggttgcgga
     1441 tggctgcctg ctgggcgcct tctaaggctt cggagaaggt cggtgctttg cggcgggcga
     1501 tgctgcgctt aatgaggcgt tcgatagtgc gcaggtgttc catttcacgg ggaatgacga
     1561 aggtcgtagc ttcgccgctc tggccggcac gaccggtacg gccgacgcga tgaacgtagc
     1621 tttccgggtc ctggggcagg tcgtagttga agacgtgggt gacaccgctg atatcgaggc
     1681 cgcgggcggc gacgtcggta gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcgcgc ttagcctggg tcaggtcgcc
     1801 gtggatgcct tctgacgagt agccgcgttt ctttaaggct tctgagactt cgtcgacgcg
     1861 gcgtttggtg cggacgaaga tgatggccaa atccggttcc tgcaggtcga ggagacggca
     1921 gaggatgtcg aacttctggc gatccggtac ttcgatgtaa gactgttcga tgaggtccat
     1981 ggtgacttct ttggtcttca tgcgaatcat gagggggtcg tgaaggaagg tttcggccaa
     2041 ttgtcggatc ggtttcggca tggtagccga gaagagcagg gtctggcgcg tgtttggggt
     2101 cgctgccatg atgaggttga tgtcatcgat aaagcccatg tccagcattt catcggcttc
     2161 gtcgaggacg atnnnnnnnn nnaatttctt tggaaggttc ggccttcaat attttttcca
     2221 ggacaggaat gccgtaggca gcggtcttac cggtaccggt ctgggcctgg ccaatcatgt
     2281 cggagccgct catggcgacg ggaatcgatt cggactggat cggcgtcggt tcttcaaaac
     2341 ccatttcgtt cagggcacgg ataatgactt cgctgatatt taaattttgg aatttctcta
     2401 acctgtactt cctcct
//
LOCUS       DAWDXZ010000274         2416 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8504_length_2416_cov_5.334604, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000274 DAWDXZ010000000
VERSION     DAWDXZ010000274.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8504_length_2416_cov_5.334604"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    322..331
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1652..1670
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          783 a          607 c          430 g          567 t
ORIGIN      
        1 ctgggacaga ccttcgaaca agcctcgcag gctatgcatt tttctgcatc cacttgtgga
       61 tatcgaaatc cttccgaatc atctttcatg tcgattgcag aaacgggaca aacggaataa
      121 caggcatagc aacctacaca atctgcctta tctgctgcaa tcattttttc tatcatgacc
      181 atctatgaac cgcccctttc acgctgttcg taaaatcttt ccaatcgccc gccgacagag
      241 attcttttcc tcttcattca tacagagttt atacataatg acgatataaa aagccgtatc
      301 aatcgcacat agaatgacaa annnnnnnnn ngacaaaatt acgccaactc aaagaaagct
      361 gcagcatata gtccagccct aatcccacca acaataacaa gaaggaaccc ttcatcaagg
      421 ataaaatatt cttccaaaat agcaccatat ccaaatgaat ccgtttcgca tagtagcgat
      481 tcatgataaa tcctgtcgat ataatcaaag ccgctgctgt tgcaacggca catccccaac
      541 cgccccagta gcttgccaag gggaaactaa ccacaagagc agcaaaggca caaacagaat
      601 acatggtcat gcggtaacga ttcagattca tggcctgaag aatagaaatt cccacattct
      661 gtaaaagcgg aatcatgagt gccagcataa gcagcaccac aataggataa gctcccaaat
      721 aatcaggccc ggcccagagc gtgataaatt ctcgacctac caaaaaaaac atagtacaga
      781 tatatcccac taccagatat tggagccgtc cgattcgcgt catcaatttg ctcaattccg
      841 atacaggaac cttgccggct accatcatag ttactttcgg cagcataacg ccgctgattg
      901 caacggaaaa gttcatatac attatcatga actgcatggc aatggcataa acagcaactt
      961 gaacggtccc ggaaacaatc cccaaaacaa actgcccggt accccaataa attttatcca
     1021 taacgacatt caaaaaaata aagaatgaat acgtcgcaat ttctttcaaa aatggtatct
     1081 caaaatgccc gcgtgcgaat cggatatgga gatagcgaaa acaatagaac gcattggcta
     1141 gcaggcaggc aaaattaagt agtgtagaaa caactaccat cataaccgag ccataaccca
     1201 tatacagaaa aggcaatact aaaagagggt tgacaattac ccgcaggata ttcgataccc
     1261 tcaaaaaaat aaatttctca taggcattga ggatagaagc aaagacactg aaagggaagg
     1321 aaaaagcaaa gttgcagatt aacagaatca tcatgatcct agcccggtgc atttctccat
     1381 cagtcaatgt cgcaccaaac agattgtcaa tattgctgta aaggatagct cctataatga
     1441 tacaaagaag gccgatgagc gaatatacac ctaaaaaaag gccattcagt tctgcttctc
     1501 gctgtggcgt accgtcgacc ttatttttag ccgtataacg gacaatggta ttccctaacc
     1561 ccatatccag tacgctgaga taaccaacca tggagccgat caaagagtac agaccatact
     1621 ctgactgccc caaaagacga agcatgaccg gnnnnnnnnn nnnnnnnnnn atccgacaac
     1681 cacattggca atgacattta cataggacag aatagccccc catcgcctct gattcatgtt
     1741 tcaaagcccc tttcaacctt attgtggtct atgaaaaatt ccatcaaagt aatttttaga
     1801 ccgatttcgc tccctatcca gaaccgcgtc gacatcggac cagtcaggcg cttccgtcgg
     1861 attagaaatg gcatagccat tttccgaagt ccctcttcta ttttctaggc caaaaagtcc
     1921 cagaaggcta tcgatacgag accccatctt ttctgttacc gaatttccca accgattgca
     1981 gactacaaag ggagtccgat aaagaataga aaataccgta ccatggaaag aatccgtgta
     2041 taccagactg gcatgcttaa tggcccaaat aaattcatcc acgccggtca cataatgctc
     2101 atatacattg gcatccaaga gatttaccac cgataatcca gcgtcccttg ccgtcttctg
     2161 aataacttca tccggccgtt cccctaggaa ataagtcaga atatattttt cgccatggta
     2221 ccaggcaggg atgcgactta ccttatccca ctcttccgtt gtaagcagca aggtaggatc
     2281 tacatgcact tcggccttcc ttccggaaat ttgctgtact aattcagccc cggcttgctc
     2341 gcgaagtgac aatctatgca tctcatcaaa accattttta taaagatctt ttctattttc
     2401 gggaatatct ggagca
//
LOCUS       DAWDXZ010000275         2405 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8533_length_2405_cov_8.767660, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000275 DAWDXZ010000000
VERSION     DAWDXZ010000275.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8533_length_2405_cov_8.767660"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1101..1110
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          662 a          663 c          581 g          489 t
ORIGIN      
        1 aaatgatgag ggacatcaaa cgggccctcg atccgaacaa catcttaaac cccggaaaga
       61 tctttgacat cgacgaaaaa gcctgagaac cagcgccatg gctatgactt gcaaaaatca
      121 cgactaccgt gtataataca aaacagaact gaataaaacg tatatcttca gggcagggtg
      181 aaattcccga tcggcggtac agtccgcgaa cgcagcagcg taggagccat agggctcgtg
      241 atttggtgaa actccaaaac cgacagtata gtctggatgg aagaagatgt ggcgtgctgc
      301 tttggcagcc tgccttacat tgcatagaaa agccctgagc cgttatcggc gcagggcttt
      361 cgttataagg aggtctttat tttgacgaca gaaaaacgta aattcaacac cattgaagaa
      421 gctatcgaag aactgcaggc cggtcatatc gtcatcgtca ccgacgatcc ggaccgtgaa
      481 aatgaagggg acttcatctg cgcagctcag tactgcacga cagaaaacat gaacttcatg
      541 gccagccacg gcaagggcct gatctgcatg cctatgagca tcgaatatgc ccataaatta
      601 ggcctgccgc ccatggtcag cgaaaataca gataaccacg aaacggcctt caccgtttcc
      661 atcgaccacg tcgatacgac gacaggtatc tcggctgtcg aacgggccct cacagcccgc
      721 aaatgcgccg aagaatcagc tcgtcccgaa gatttccgcc gtcccggcca caacttcccc
      781 ctcatcgcaa aaagaggcgg cgttttgacc cgcaacggcc atacggaagc aacggtcgac
      841 ctcctgcgct tggcaggcct caaacaagtc ggcctgtgct gcgaaatcat ggccgacgac
      901 ggcacgatga tgcgcacgcc gtccctgtgg gaaattgccg ataagtacca cttaaaattc
      961 atcaccatcc acgatttgca gaactactgc cgccgccacg acaaacacgt catccgcgaa
     1021 gccgtcgcta agatgccgac caagtacggc gagttcaaaa tctacggcta catcaacgac
     1081 ctcacgggcg aacaccacgt nnnnnnnnnn acgtcgccct catcaagggt gacatcggcg
     1141 acggcaagaa cctcttgtgc cgcgtccact ccgaatgcct caccggtgac gtcttcggct
     1201 cgtgccgctg cgactgcggt gaacagctgg cagccgccct cagccagatc aacgccgaag
     1261 gccggggcat cttgctctac atgcgccaag aaggccgcgg tatcggcctc atcaataagc
     1321 tcaaagccta tgtcctccag gaacagggat acgatacggt cgaagccaac gaaaaactcg
     1381 gctttgcccc cgaccttcgc gaatactgga ttggcgctca aatgctgcag gatatgggtg
     1441 ctaaggaaat ccgcctcctg accaacaatc cggaaaagat ctacggcctc gaaggcttcg
     1501 gcgtagaaat cgtcgaacgc gtccccatcg aaatggaacc gcaaaaattc gatgaattct
     1561 atttagaaac caagaagaat aagatgggcc acatccttcc ccatctccac tgatccttag
     1621 atatacaaaa cggcagggat tgccgatatt ccgcaatccc tgccgttttt tcttccctta
     1681 taacaaggaa aattgatctt tatgataatc gatgagtccg tcttttttca tcttactgag
     1741 ttccgccgat aaggcgctgc ggtcgacaga tagaaaatcg gccatctgct ggcggttaaa
     1801 gggcagtgaa aaggtcgacg cacccttatc ccgcgccaag ttcgacaagt agagcatgat
     1861 tttctgccgc gtcgagcgct gggtcaaata gcgcatcttt tctgtaagga gtcgattctt
     1921 ttcagccatg aggcccaaga gatttcccgt aaactgtgcc tcagccggcg tcaagagatg
     1981 gccgccgtgg aggacattgg ccacatagat ctgaatgatg accgcatcag aggccgccac
     2041 gacatcgacc gtcgccggcg cctcgggaag acaggcaaag gcttcgccaa aaaagaagcc
     2101 ttgtgggatt tgagacagaa tattccgatt gccccaataa tcttcctgaa tgatatggac
     2161 cgaaccttcg agaaccagcc cgatataggt gaccggatct ccggaataaa agagaaactc
     2221 ccctttttta tagtttttct gcctgacttt taaatcctgc aaaagcggca ggacatcgtc
     2281 cttggcgacg ccgcgaaaaa gaggcgattt ttcgataagg gaaagatgtt gttccattag
     2341 cagcacctcc tgctgaataa gttggaaaaa caacggcaat atccgtcaac ataacgtata
     2401 ctaac
//
LOCUS       DAWDXZ010000276         2405 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8532_length_2405_cov_10.740426, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000276 DAWDXZ010000000
VERSION     DAWDXZ010000276.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8532_length_2405_cov_10.740426"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          587 a          647 c          614 g          557 t
ORIGIN      
        1 ctgcagctcc attcgcttgg ctaaccgaga aacgaagtta atgaagtttt tcatggccaa
       61 cgtcgggaag cccttatcga cggagcaaat tttcgacaac gtctggggcg atgaaggtga
      121 cgtcgacgac ggcatcgtct ttatgtacgt ctcctattta cgggaaaaac tggaagccat
      181 ccaggccgat atcgaaatcg tcggcgaaag aggccagacc tacttgctct cggtaaaaga
      241 aaggaaatag gcttatgaac atgatcaagc gcttgcgccg caaatttctc atcatggccg
      301 tcatcggcgt cctggtcggc ggggccttag gactcatcaa cgccattgcc tatatgcgca
      361 tgaacgccca ggtcatgtcc gttttgacct atatcatcca taatgacggc gccgtgcctc
      421 agaaaatcgg cggtgacgac agcagtttct tcagcgatcc gtcgtggacc gaagagacgc
      481 cggaatttgc ctatcaaatc cgtttcttta gcgtcctcgt caacggcaac ggctatgccg
      541 aacacatcga tattcagcac attgcagctt tttctcagcc ggaagccatc gaatacgctc
      601 ggacgactgt cgaagagtcg cgcgagaccg ggcagaaaca gggctttttc aaaaagggac
      661 gggcgaccta cgcctatatg ataaaaccca atgacgacgg cagtttcctc atcgtcatca
      721 tggacgctac ccgcgatgcg gcggccgtta cgtcctttat gcgctactcg gcctggttcg
      781 gcttcggctg cgtcgtcgtc tacatcctca ttttggccgc cctctgcaac atagccatcc
      841 ggccctttgt ccagaatatg gagaatcaga agcggttcat taccaatgcc ggccatgagc
      901 tgaagacgcc tattgccatc atttccgcca atgctgaagc cattgagatg ctgaatggcc
      961 aaagtcagtg gacggacagt atcttaaagc aggtccgtcg cctgtcgaac ctcattcaag
     1021 atttgatcct gttgtccaaa atgggcgaac ggtcgcaagt cgatctagtc attaccgacg
     1081 tcaacttctc ggatacggtc aaaaccgtgt cggactcctt ccagcagctg gctatcgacg
     1141 ctgaaaagac gctgactacg gctattaccg acaatgtcag actcaaaggc gacagtaaat
     1201 gcctgtatga attggtcaat atcctcgtcg acaacgccgt caaatactgt gacgacggcg
     1261 gcagtatcga agtggccctg cgccggtcac ggctgggaaa tggcgtcgtc ctcgacgtgt
     1321 ccaataccta tgtcgacggg gccaatgtcg actattctcg cttcttcgag cggttctacc
     1381 gcggcgacgt gtcccacaac agccaaaagg ccggctacgg tatcggcttg tccatggctg
     1441 aggaattgac caaattgatg ggtggaaaga tatttgcgac ctataaggga gatcggatta
     1501 cctttacggt ccgtctggga taatcttttg tccgacaacg gcagtatctg tgctataatg
     1561 aggacgtgac gaattttact caggggggaa gactatggaa atgcaaatgg aatatattcg
     1621 gaaactgccg gaaccgcagg aattgatgga acaattccct atggatgacg gctcaaaggc
     1681 tgttaaaaga cggcgtgatg aggtcatcca aaatatcctt gccgggaagg atgatcgatt
     1741 cctgctcatc atcggtcctt gttctgccga taatgaaaag gctgttctcg attacatcca
     1801 tcgcctggtc aaggtacagg accgggtgaa agacaaaata ttcatcattc cccgcgttta
     1861 caccagcaag ccgcggacca tcggcgtcgg ctacaagggg atgctccacc agcctgatcc
     1921 ggaagggaag gaagacatgc tgggcggcat catggctatc cgcgaactca acgtacgggt
     1981 cgtccgggaa acgggcttta cctgtgccga agaaatcctc tatccggaaa ttttccgtta
     2041 cttatcggac cttttgtcct atgctgccgt tggcgcccgc tctgtgttca ccgcatgatt
     2101 gccagcggcg tcggcattcc tgtcggcatg aaaaatccca cgtccggcga catttctgtc
     2161 atgctcaact cgattgaagc tgctcaacat tcccaaaatt ttcttttccg cggctgggaa
     2221 gtccggacga ccggaaatcc catggctcac gccattcttc ggggctacgt cgacagcttt
     2281 ggcaatagtc ttccaaacta tcattacgaa aaccttcagc gcctgtatga agcctacgga
     2341 aaacgggacc tctcccatcc ggccgtcatc gtcgatgcca accatgccaa ttcgggaaaa
     2401 cggta
//
LOCUS       DAWDXZ010000277         2399 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8566_length_2399_cov_6.546502, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000277 DAWDXZ010000000
VERSION     DAWDXZ010000277.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8566_length_2399_cov_6.546502"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          621 a          633 c          546 g          599 t
ORIGIN      
        1 cggtttaatc gtgctgaggt cgatatagat gaagccgtcg taataggcta cgtcagccgg
       61 attcagttct ttataggcat cgccgcggcc gtgctgagtg agataggctt tcgtatcttc
      121 gtcggtccgc catacggaag acaagcacgt cgtttccgtc gtcatgacgt cgacgctgtt
      181 gcggtagtcc gtatccatgg aagcgatgcc ggggccgacg aattccatga ccttattttt
      241 gacgtagccg ttcttaaaga cggctccgac gatggcaatg gcaatgtcat gagggccgac
      301 ggccggattc ggttttcccg taaggtagac gccgacgacg ccggggtagg caatgtcata
      361 cgtatcttcc aggagctgtt ttaccagttc accaccgcct tctccgacag ccatcgttcc
      421 caaggcaccg tagcgggtat gactgtcaga accgatgatc atcttgccgc agccggccat
      481 tttttcacgc atatactgat ggatgacagc gacgtgaggg ggcacaaaaa taccgccgta
      541 ttttttcgct gccgaaagac cgaatacatg atcgtcttca ttaatggtgc cgccgacggc
      601 acagagtgag ttatgacagt tggtcaggac gtagggcagc gggaaatgat ccatccccga
      661 ggccttcgcc gtctgaataa tgccgacgta ggtaatatca tgagacgcca tagaatcgaa
      721 tttaatcttc aaattatcca tcgatgctga cgtattatga ttttttaaaa tagaataggc
      781 aatcgttccc ttctttgcgt cttctttgga aacagtcttt cccgtaagct gctgaacctt
      841 agccgcttcc tgttccggta cgatatcctt accgttaact aaatagacac caccatcgta
      901 taactgaatc atacgtaacc cctcctttgt catcgctgac gcgacggctt ttcacaatat
      961 gaaatttagt aaaataattt tcacaatata aaagttattc ctctatattc atactcgaag
     1021 gaaagagagg aatcaaattg gataaaaata tttatttagc ccaattttac ggcaagtcac
     1081 agaactacac gcagattccc cgcgaatggt tttccaagta cggtgtaaag cgtggcttgc
     1141 gtaatgaaga tcataccggc gtcctcgtcg gtttgacccg cattgccgat gtcgtcggct
     1201 accgtcgcga cgaaaatgac caaaaaatcg actgtgacgg catcttatac taccgcggca
     1261 tcgatattcg cgagctggtc aaagaaacga attataaagg ttatttatac gaagaagcga
     1321 gttttcttct cctcttcggc tacttgccga ccgaagacga attgaaagcc ttctgtaagg
     1381 tgctgcaaaa cggatatgat ctgcccgatg actttttgga aatgaacctc cttcgcctgc
     1441 cgggcaaaaa tctgatgaac aagctgcagc acgccttgtt gaccttgtac aactatgatc
     1501 ccgatccgga caatacggat gtatatcaga ccctgcgcaa agggctgaac atcatgagca
     1561 agctgccctc cattgccgtc tatgcttata tgtccaagat tcactattac aatcgtaaga
     1621 gtctggtcat tcactaccct cgcaaggact attcgacagc cgaaaatatt ttatccatgc
     1681 tgcgcccgga cagcaaattc tctcccaaag aagcgcagct cctcgatctc ttgctctttc
     1741 tccatgccga ccacggcggc ggtaccaact cgacctttac caatatcgtc gtttcctcta
     1801 cagacaccga tatttattcg agtatggcaa gttccgtcgg cgcccttaaa ggaccgcgtc
     1861 acggcggcgc caacatccgt gtctccttga tgatggaaca gatcatcgat gccatcggca
     1921 tcaaggctac ggacgaccaa attgtggaag tcatccatca aatactcaat aaaaccttca
     1981 agggaagcga caagggcctg gtatacggtt tcggccatgc cgtctatacc ctgtccgacc
     2041 cccgagctga aatcatgcgt cattactgcg gcatcttggc caaagaaaaa ggcatgaacg
     2101 acgtcttccg cttctacaac cgttttgaaa gattggccat ggataccctt accaaagaaa
     2161 aaggcgtcac catgtgcagc aacgtcgatt tttacagcgg ctttgcctat aatatgctgg
     2221 gcattccccg cgatttatac acgccgctgt tcgtcatcag ccgtatggtc ggctggctgg
     2281 cccacaacat cgaacacaag ctctacgaca accgcatcgt ccgtccggcc gccaagtacg
     2341 tcggcgacct catggattat attccccgaa aggacagata accatgaaga caatcaccg
//
LOCUS       DAWDXZ010000278         2377 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8635_length_2377_cov_8.568906, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000278 DAWDXZ010000000
VERSION     DAWDXZ010000278.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8635_length_2377_cov_8.568906"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1671..1680
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          541 a          588 c          597 g          641 t
ORIGIN      
        1 cgtcttcgac cggcatcaag aacggctggt ccgtcggacg ctgcggggtc gggatgtatt
       61 cgtcaacggc atccatcaat ttgaggatgg acggcgtgcc gatttcgctg tcgtcgcctt
      121 cgagagcttt gagagcggaa cctacaacga taggtacatc gtcgccgggg aagtcatagg
      181 agctgaggag atcgcgaact tccatttcaa cgagttccaa cagttccgga tcgtcaacct
      241 ggtcagcttt gttgaggtat acaacgatag ccggtacacc aacctggcga gccaagagga
      301 tgtgttcacg agtctgcggc atcgggccgt cagcagcgga tacgacgagg atagcgccgt
      361 ccatctgagc agcaccggtg atcatgttct tgacatagtc agcatggccc gggcagtcaa
      421 cgtgtgcata gtgacggttg tccgtttcat attcaacgtg tgcggtgttg attgtaatac
      481 cgcgttcacg ttcttccgga gccttgtcga tatcggcata gtcttcgaat ttagcgtagc
      541 ctttatcagc caaaactttg gtgatagctg ccgtcaatgt cgttttgccg tggtcgacgt
      601 gaccgatcgt gccaatgttg acatgcggtt tggttctttc aaaatgttct ttagccattt
      661 gttgttcctc cagtgtgagt aaattatatt aggcgattag cctttttcac tgatgacttt
      721 ttcagctacg ttcttaggaa cttcttcgta gtggtcaaag gtcatcgtat aaacgccacg
      781 gccctgcgta cgggaacgca aatccgttgc atagccaaac atttctgcca ggggtacgaa
      841 ggcgttgata tgttctgcac cggagcgcat ttccatacct tcgacacgac cacggcggga
      901 gttgaggtcg ccgatgacgt cgcccatgta ttcttcagga acgacaactt cgactttcgt
      961 gtacggttcg aggagaaccg gatcggcttt gcgggcgcct tctttaaagc ccatagaacc
     1021 tgcaatcttg aaggccattt ccgaggagtc gacttcatgg taagagccgt catatacggc
     1081 aactttgatg tcaaccattg ggaagcctgc caaaacgcct gtttccatag cttctttaac
     1141 accagcttcg acagaaccga tatattcctt aggaatagca ccgccaacga ctttgttttc
     1201 aaattcgtag cctttaccct gttcctgcgg aatgagttcg agccagcagt caccatactg
     1261 accacggccg ccggactgac gtttgaagaa gccgcgggat ttgatttctt tgcgaatcgt
     1321 ttcgcggtaa gcaacctgcg gtttaccgac gttgcagtct accttgaatt cgcgggacat
     1381 acggtcgacg atgatatcca aatggagttc gcccataccg gagatgatgg tctgacccgt
     1441 ttcagcatcg gtcttgactt tgaaggttgg atcttcttca gccagacgag ccagggcaat
     1501 gcccattttt tcctggtcag ctttcgtctt cggttcaaca gcaacggaaa taaccggatc
     1561 ggggaattcc atcttttcga ggatgatcgg attcttttca tcgcagagcg tatcacctgt
     1621 cgtcgtatct tttaagccta cgatgccgcc gatatcaccg gtataagctt nnnnnnnnnn
     1681 atatcatgat actttttagc ttcttctaat tcgtcttccg ggatgtcaac gacttcgatt
     1741 tctttaccga gagcatcatc atagatttca gctttcatgg taatgaggtc gataataccg
     1801 ctgaacgtcg attcagcacc gatcgggagc tgaatagcga cggcattggc ctgaagacgg
     1861 tctttcatca tttcgacaac gttcaggaag tcagcaccgg tcgtatccat cttattgacg
     1921 aatgcgatac ggggaacatg gtaatgttca gcctgacgcc ataccgtttc cgactgcggt
     1981 tctacgccgc ctttagcgga gaagacagca acagcgccgt cgagtacgcg cagggagcgt
     2041 tcaacttcaa ccgtgaagtc aacgtgtccc ggggtatcga tgatgttgat gcggtggtct
     2101 ttccaatggg ctgtcgtagc agcagacgtg atcgtaatgc cgcgttcctg ttcctgagcc
     2161 atccagtcca tggtagcagc gccgtcatga acttcaccga ttttatgaac tcggccggta
     2221 tagaacagaa tacgttcggt tgtcgtggtt ttaccagcat cgatgtgagc catgatgcca
     2281 atatttctcg ttttttccaa agaaaattct cttggcacgg ttttttccac tcctcaaaaa
     2341 ataataaata tcgtaaatta ccaacgataa tgagcga
//
LOCUS       DAWDXZ010000279         2375 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8639_length_2375_cov_7.108190, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000279 DAWDXZ010000000
VERSION     DAWDXZ010000279.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8639_length_2375_cov_7.108190"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1171..1180
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          530 a          622 c          603 g          610 t
ORIGIN      
        1 gcaaaagcct gcgggtcttg attcatatcg acgtcgacat actcttgcat ccatgttaaa
       61 gaacttttca ttatcttttc cctcctgaaa ctagaactgg ttcaagaacc gcatatcgtt
      121 ttcatagaac agacgcaggt cgtcgatgcc gtatttcagc atggcaatac gttcgacgcc
      181 catgccgaag gcaaagcccg taacgacgtc cggatcgtag ccgttcagcc gcaggacgtt
      241 ggggtttacc atgccgcagc cgaggatttc cagccagccc gtatgggagc agacacggca
      301 gccttcaccg ccgcacatga cgcaggaaat atcgacttca gcactcggtt ccgtaaaggg
      361 gaagtagctg gcacggaagc gaactttcgt gtcttccccg aagatgtcgc gaaggaagtc
      421 ttcgagcatg cccttgaggt cggagaagcg gatgttctta tcgatgatca tgccttccat
      481 ctgatggaag accggcgaat gtgtcgcatc gtagtcgcag cgatagactt tgccgggggc
      541 aatgatttta aagggttcat tgggtttatg ggactgaaga gtccgagcct gcatgcctga
      601 cgtatgggtc ctcaggagga tgttgtcggt gatatacatc gagtcctgca tatcccgtgc
      661 cgggtggtct tcgggcagat tgaggcactg gaagttatag tagtccgatt cgatatcggt
      721 cccttcgacg atggaaaagc ccatgcgcag gaaggagtct tccatgaggc gcagggtctg
      781 atgaagggga tgttcgtgac ccgtaaccgg tttacgtccc ggcagggtga tgtcgatttt
      841 ttccgattcg attttcgcag ccagttcctt ggctttcact tcttccaact tggcattcag
      901 ctgttcttca atggcatttc gtgccgtatt gactagctgg ccgacttttg gccgatcttc
      961 cttggaaaag tttttcattt ccttcataat cgccgtcagt tccccttttt tccctaagaa
     1021 cttgacgcgg atatcttgga tatcctgcaa atgttcgctc ttggcgagct cttccattac
     1081 ggccgtttta atggcctcaa tcttttcact gatcatggtt ttcctcctac tggcaagtac
     1141 ataaaaaata gactcccgcc ccctaagggg nnnnnnnnnn cttccgttac cgggtactca
     1201 atccacgccc cggatttcct gtaaacggaa gtcccttcat gccctcttta tcatcgtcat
     1261 aataaatcgc tattcaattc acgatacatt gtatcagtaa agtgtcttat tggcaataac
     1321 taagcgctga atctggttgg tgccttcata gatctgcatg atcttggcat cgcgcatgta
     1381 tttttcaacg gggtattcct tgatgtaacc gtacccgccc ataacctgaa cggcatccgt
     1441 cgtaacctgc atggcgacgt cggaagcgtt gcatttggca atggcagctt cacggctgaa
     1501 gtccatgccc tgatccttca tccagcaagc gcggtggacg aggagtcgag cgccttcgac
     1561 cttcatggcc atatcggcga tcatagcctg gaccatctgg aaggaagcaa tcggttggcc
     1621 gaactgtacg cgaaccttgg cgtatttaca gcaagcacgg aatgccgctt cagcaatgcc
     1681 gacggaaacg gcaccgacga aaggacgagc ggcatcgagg gttgccatag cgatacggaa
     1741 accgctgcct tcgcggccga gacggtcttc gacaggaacg aagcagttgt caaaaatgag
     1801 ttccgtcgtg ttggacgcac ggatgcccat cttattttct tctttaccga tggtaaagcc
     1861 cggcgtgccc ttgcggacga tgaacggcgt aaggccgcga ataccgccgg ttttgcgggt
     1921 attggcaaaa acgacaaaga tttcagccaa accgccgttg gtaatgaagc acttagtacc
     1981 attcaagaga tagcctttgc cgtcttccgt cttggtagcc cgtgtcgata cggagcctgc
     2041 atcggagccg gttcccggtt ccgtcagggc aaaagcagcc aggttgtctt cattgatgac
     2101 gtcaaaatag cgtttcttct gatcgtcgtt gccggccaag atgaccggat acgaagccag
     2161 tgcattggca gctaccgtcg tagcgacgcc gccgcagcct ttagctaatt cttcataaac
     2221 accagcaatg gtaatggcat ccagtccggc tccaccaaat tcttccggaa tagcgacgcc
     2281 caatagtccc tgtgctgcca atttttccaa aagtccttca tggagctttc ccgtttcatc
     2341 catttctgca gcgtagggga cgatttcttt ttgca
//
LOCUS       DAWDXZ010000280         2371 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8651_length_2371_cov_8.729275, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000280 DAWDXZ010000000
VERSION     DAWDXZ010000280.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8651_length_2371_cov_8.729275"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          551 a          572 c          585 g          663 t
ORIGIN      
        1 attcagctca ccatacgtat cgatgtcttc attatatttc atattatttg tcgccatatt
       61 aatcgctctc ctacgccctc ctgccgcaaa ctccgaaatc cttcgtcggt agggctttaa
      121 aaattttcca ttcatatcat tcactgttat tagtttatcg aatataataa aaatttataa
      181 gccccctatg gggataataa agaatgtttt tagcatttct cgggaaaatg ccgatactaa
      241 aagaaatttt caaaaaaccg tcattaacaa atcaatttgc cattctttag tcatcaaatc
      301 tttgaatgta aggcttaatc tgcaattatc atcgtccaat atcccccaat agggcttata
      361 tgtatacatt ttttaaggta ttattaaagt tatgaagaaa acgaagaaag gatgagacat
      421 gttgaattat acactgcagc gtttcctaca gttgattccc attctgtttg ggatcacctt
      481 cctgtccttc ctgctgatgt atatggcagg cagtgatgcc gtaacggaaa tgtataccaa
      541 ccgcggcgta gaggtggccc aagaaatcat cgatgccaga aaagccgagc tgggattgaa
      601 tctgcccttc ttccggcagt atgcgtcctg gcttcaggcg atgctgcacg gaaatatggg
      661 tatgagctat gtgacgggcg agcccgtgct ggaatccttt ctgcgaaagc tgccggcgac
      721 gatattgctg acgacactat cgatattgct gacggtagtg atttccctgc ccttaggcat
      781 tttggcagcg gtccgtcaca ataaagcgga agatttgatc ctgcgctttt tcagctttat
      841 cggcaatgcc atgccgaatt tctttgtggc catgctgttg atgcagttgc taggcatcca
      901 attgaacctc ttgccggtca tctccggcgg cacggattgg cagagcacca tcatgccgac
      961 gatgaccctg gccattgcca tgtcggcgaa gtatttgcgg caggtccgtt ctaccgtact
     1021 ggaagagctc aataaggatt atgtgttggg ggccaaggcc cgtggggtca gccgtaaggt
     1081 tattctttgg aagaatgtca tgaagtccgc catgctgacc attatgaccc tgttaacctt
     1141 gtcgatcggc agccttttgg gcggtacggc cattatcgaa agcatcttca tgtgggatgg
     1201 cgtcggcaag ctggccgtag atgccatcaa tatgcgtgac tatccgttga ttcaggctta
     1261 tgtcatgtgg atggcgatca tctatgtcgt ggtcaatctg atagccgatc tgctgtatca
     1321 tcatctggat ccgcgtatcc ggctgggggt gagccgcaag tgaacgacgt tacggtacga
     1381 attcaaaccg tgaaaaagga ccgactcaaa tataaactaa tcgccttctc gatcttggct
     1441 ttattactga ttgccgcagc ctttttcagc gaatccctgt gtccgtatga cccgtatgaa
     1501 cagaatctgg ccattgccaa ggcagcgccc tctgccgaac atctgctggg taccgaccgt
     1561 ttcggccgcg atatgctctc aaggatcatt gccggcagct atacgagtat tttttctacc
     1621 ctcctgttgg tcggttttat taccgtgttt ggcagtctca tcggcattgt ctgtgcctgg
     1681 tcgggaaaat ggattgatac actgctcatg cgcctggccg atatgtttct ggccttcccc
     1741 ggcctggtct ttgccttagc cgttgctgct gtattaggcg gcggtgtcca taacgccatc
     1801 ttagccctgg cggccatcag ctggcccaaa tatgcccgtc tggcccgcag tcaggcctta
     1861 gcccagcagt cggcaaccta tatgcaggcc gctaaaatgg caggaaacag tacatttcaa
     1921 atgatttaca agcatgtcat tcccaatatt tcaggtccca tcttggtcac ggccatgctc
     1981 gatatcggca cgatgatgat ggaattagcc gggctttctt ttctgggact gggcgcaaag
     2041 ccaccgattc ccgagtgggg cagcatgatg agtgataccc gtaatctctt ggcgactcat
     2101 ccttgggtaa ccctgtctcc cggcgtggct atctttgtgt cggttatggt tttcaatctc
     2161 cttggggata cggtccgtga ctggctggac ccaaagaatc ggaggtaagg atgagcggca
     2221 tcatacgata tgatcaggta gatatcagtt ataacggaag gcgggtcgtc caagatgtca
     2281 gctttgacct tcaagccgga gaaatcctcg gcattgccgg cgaaagcggc agtggaaagt
     2341 caacgttatt gaaggctgct atgggcctgt t
//
LOCUS       DAWDXZ010000281         2371 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8649_length_2371_cov_9.354059, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000281 DAWDXZ010000000
VERSION     DAWDXZ010000281.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8649_length_2371_cov_9.354059"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    649..658
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1732..1741
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          438 a          630 c          709 g          574 t
ORIGIN      
        1 atgcggttat tttcgcttcc tgccggtgcc gcagcgatgt tctgcaagtg aagcaggtcg
       61 gcaatgccgc cggcaatgct ggtcggaagt tccagcgaca cggcactggg cgtggcatga
      121 atgatggcaa tgcgcaggcc ctgtttgggc acagcatcgg tgatggcttt aatttctgta
      181 tcgagctttt catcgacggc tttggcctgc tcttctttgc cgtagacccg gcccatgacg
      241 tccagattgt gtttgacgtc gtcataagtc ttggtgcgca gcaagatgac gggaatgccg
      301 ctgtcttcga gctgggccgc caatttttca tggtggtccg tactgccgat gacgacgtcg
      361 ggctcaaggg ccgtcacttt ttccaggctg acgctgtaga ccgggcccac gtcgggcacg
      421 tctttatact tttccggaag ggctgcgtct tccgacttga tggcggccct tccgacgaga
      481 ctgccgtcga gggcatcggc aaagtttagg agcgacgtcg acaagacgac gacgcgctgc
      541 ggtttctgcg gcaaggtaac ggatttgccc cggtcatcgg tgatgacggc ataattttcc
      601 ccggatgcct tttcttgaga cgtcccttgg ggaccgcagc cggccagcnn nnnnnnnntg
      661 ttggccgcct tcacgtaaat cgtcgtgttt tcgtcgggcg aataggatgc gctgccgttc
      721 cagacgacgt aagaattgtt gctgtaatac gtgtcgttgc gccccgttcc ggccgtcacg
      781 tcgacgttgg cctgccattt tccctgatgg taatggagac cggcgcggta gccgttaggc
      841 tgattgtacg tttcatcaaa gcctaagccg tctcccgtat cgactttggt gtggatgtag
      901 gaatagccca attcataatc ccacttgtcg ttgatggtct gcttgaggga cagttcaaag
      961 ccccgtttat cttcttcatt gaggttttcc ggttcccgcg gcgtgacggc ccggttccag
     1021 cgaatgacgt tcgctaaatc gctgtggaag acgctgcctt cgagaagggt cttgggcccc
     1081 atgcgatact ggaatcccag ggtctgagtg taccccgttt cgggctgcaa atcctcattg
     1141 cccatggccg taatcttgcc gcgggacgtc gtcacataga actgatcgtt caggcgcggc
     1201 ggcgaaaaga cgcggctcca cgacgcatag acgttgaagt cgtcattggc cttataattg
     1261 acggccaacc gcggcgtctg gtggacaccg tacgtgctgt tgtcatcgag gcgcaggccc
     1321 ggcgtgaccg tcaacttccc gaaagtcatg gcgtcttcgg cgtaaaaggc caggttgtgg
     1381 cgtttcttgt cgatgtaatt ggcctcgtaa ttggtgccca tgtcttccgt ccattcggcg
     1441 ccggccgtga cgtgatgagc gccgaggtgc cagttgttct gccattcaaa gccctgagta
     1501 cgggtaaaat agtggttggt ccaataggtc cgctggtagt cgttgaagta gcgcaggaaa
     1561 ccgggctgag cctcattttt cttaaaatta taggtaaggc tgtaattatt gatgagccgt
     1621 tcgatgggct tattggcact gaggtaccat cccgaaaggc gttctttttg aagggctttc
     1681 ggcagcgacg acgaataaga agcaaggaag gtcgaaccgt ggccgtgata annnnnnnnn
     1741 ntgatggtaa gccttaaagg tcaacgacgt gtcgtcggtc aggtcctgat cgaggcgcag
     1801 ggacaaggac tctttggcaa tgtcgccgcg ggatttatcg gcattgaggc cgccgggaaa
     1861 ggcgtaatac tttcgccgct ccatgtcgcc gctgatgagc cagcttgtct tgcccgaacg
     1921 gccgctgttg gtcagggtgt agccgtgcat gccccacgat ccggtgtagg catcgagaga
     1981 cgactcgttg cggtcgccct ttttggtgac gatattgatg acgccgccca cggccccgct
     2041 gccgtagcgg acggaaccgc tgcccttggt gacttcaatg cgctcaatag caccgacacc
     2101 ggggaggttt gccaaatcga cactcccccg cccgtagccc gatccctggg cattggccag
     2161 actctgaccg tcgacgagga tcaagacccg gtcgtcgccg tttaatttga cgtaggacga
     2221 aatgcccggc accatctcat tgacggtaac gcccgtgacc cgctgaaggg cctgctggac
     2281 cgacgtatcg cggtgttcgt ccatctcctt ggcggtaata atggtcgtat ttgattgatt
     2341 ggcctcaacg ggaggtatgg agggctcttt a
//
LOCUS       DAWDXZ010000282         2353 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8704_length_2353_cov_8.441253, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000282 DAWDXZ010000000
VERSION     DAWDXZ010000282.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8704_length_2353_cov_8.441253"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1029..1038
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          724 a          545 c          439 g          635 t
ORIGIN      
        1 tatcactcat tatagcgcat aattatatca gaaccaatac ttcgccaacc atagatttct
       61 ttatttccaa tcgagccatc tgtatccgct catcatgtaa ctaaggtaag gaacctataa
      121 aaacggtcat tgactctcat ggggagcgtg ttattataaa aatgagtttt acgaatcgaa
      181 aggcagcaac tcccagcata aggattcatc tgtcaaattt tttccaaaag aagggattct
      241 attcatgtca cattttaaaa ttttaggagg agatttttct aaagaaacat cgcttttaac
      301 tacgttcggc ttgatatttc agttatcagt ccccaggccg ggattcacgt taaaatccga
      361 tacctatgag ctgaaaaata atatcgccaa agttgaacaa atcacagagc agaataaggc
      421 gaaagtctta tccaaggccg ggtggacaac cttaggtgcc gttgccctgg ggccggtcgg
      481 acttcttgcc ggcctgattt ttggcggaaa taccaaatac atatgcatgg ccgtgaaatt
      541 aaaaagtggc caagaatttc ttgctgaatg tgatttacaa acctatcaaa aattttatga
      601 cgcttattta gaaacccgtc gcacaaccga agagtcctac gatgaagatg tggatgagga
      661 cgaagacata gacgaatcaa caactgcaga tgattcgata cctatggaca catactccgt
      721 taccaaggac ccggccgcct tgctcaaaca attaaaagaa cttaaagacg aaggaattat
      781 ttccgaagat gaatatagaa ccaaagcacg aaaaatcttt gataatatct gactccatat
      841 ccaagacatc tattgctttt tccctcttta tcgggcatac tcaaagtaat accgacactt
      901 agaaaggaga tttcttatga cggatcttac tatcaacgta gatgaaaaac tcaaaaaaga
      961 tgccgaagcc gtattcaacc gtttagggat ggatctgcct tctgccatta ccctgttttt
     1021 aagaacctnn nnnnnnnnat caaaaaccac ttggtgaggt caatgaagga aaacggcagc
     1081 ccccataaag gctgccgttt ttccttcaaa actcagtcat ctcacttcac gacgacatgc
     1141 acttttttcc cacaaaaggc aatgccatat ttccatacca tcgagatgcc acgctgttca
     1201 aaagctgttt catattttcg atcttcaatc tgctgtaatg cttcttttgc tttttcttct
     1261 aactgttttt ccccatcggc actcttaaat tccatgatga caccggcttt tgaaatatct
     1321 ttcggaaaaa tggccaggtc aaacctccca tatccgcttt catgattcga ttcgacacca
     1381 taatcttttc ctaaaaacaa ggccgtcatc cccaacatga atccgtgata aaaactttct
     1441 ttattcgcag tatcgtacgt actggcaaaa caaaggagaa tccgctgaag ctgataagag
     1501 aagtcagcgc ccttcccgtt cagcaataaa tcgaagagcc cgtcaaaggt atcccggtct
     1561 gccccatcgg ccaaagaatt caaaatctcc atactataca ctcttttaat ttcttcattg
     1621 ggaatgcgca gtgaataccg ctcataactg gtctgtacat cggaagctac cgttaaataa
     1681 cccgttgtaa gcaacagcgt gtagagtgca ctttggtccc tgccgattcg ttcataaacg
     1741 acaccttcat tcaaactgac agatatcggc tcgtcatgta aaagtccctg caatgatttg
     1801 atacgcaaat aatcagcgta ggaaaggagc tcccgcagga tggcgttatt cgacgtgtta
     1861 atccaatatg gcataggaat acagtgatgg ttgatataat tgatgaccga ccatggatta
     1921 tagatttcac tggttccaat acggtatcca tcataccaat tttgtaattc cggaagggct
     1981 gaggtgatgt tcagttcagc agccatattt gcgacttctc cgggcgtaaa tccaaaaaca
     2041 tcgctatagg tatcactcaa tacagagcat acctctaaat tatttaatcc gctgaaaaca
     2101 ctttctttgg caatgcgaag caccccggcc agaacggcaa aatcgaggac gggattggtt
     2161 tttaaggcac tgcctaaaaa caggcgcata aaggccatgc actccgtata aaagccacat
     2221 acccaagcct gctgaatggg agcatcatat tcatcgatca gcagaataac cggcatttgg
     2281 taataagctt ccataatttc catcaatcga gtcaaagacc cggccaattc attttccgtc
     2341 gcctctcgtt tga
//
LOCUS       DAWDXZ010000283         2348 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8725_length_2348_cov_5.900567, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000283 DAWDXZ010000000
VERSION     DAWDXZ010000283.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8725_length_2348_cov_5.900567"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          475 a          545 c          726 g          602 t
ORIGIN      
        1 ggtgcagtgg ggcatgacgt tgatcgtata gtcttccttg ccctgttcct ttaacttctg
       61 ttgggccaga ttccacgctt cttcgacggt ggctgccggg atttggccgg tccggcggat
      121 gtcctcgaag tttcgtggca gggtgaccag gatgactgtg tttttatggg ccaggcagaa
      181 caggttgaag gcgacgaaga aggggatggt gaagtgagcc ctgagggccg tttccatttc
      241 ttccatcgta tcgtatttga agctgtcgag gtagatggcc ggttctttga tgtcgcgggc
      301 ttccatgatg gcgatgatga tgcccccttc cttgacggcc atttcagccg ggtcatagca
      361 tttgctgccc tggtagaggc tgacgtcact gggatatccg ccggcgcagg cgaagacgac
      421 atccgttttt tgcttcatgg ggacgcgctg gatgtcgagg acggcctgcg ttccggcatg
      481 ccaggcttca taccaatcgc cgccgaccat gcggcagatt tcgccgtcgg cattgatgat
      541 ggaatggacg aggaagcagg gattgatctt gctgcaggct tcgaccatgt catcgttcaa
      601 aggattgttt tcaatcatga gggcccgggt cttgggattg acaccgtgac cgaattcgtc
      661 ggccaaggca tggcagtgat tgcgttgaat cgtttcgaag ccggatacgc cgggcaggat
      721 gagcttgcgg ccgccgccga aaccggcaaa gaggtgggtc gtaatgccgt cgatgatgat
      781 gaccttgtcg gcatcgacga cgtgtttatt cagctttagc ggcgtgccga gcttggtcgt
      841 cccgcagtcg acgagggtcg gatccatgca gtcgtgctga taagtcttga tgcggtcgat
      901 gacttcctga ccgcagacgg cgacgtcttc ttccggcgtc tgggcccggt gggagccttg
      961 ggaaaagacg atatagatgt cgtcgtcggg aatgccggcc aagtttaatt catcgatgac
     1021 gtagatgaga aattggttgg aatgattcca agtgcgcgta atgtcggcga cgaccaggca
     1081 gactttatca ccgggctgta ccttttcggc cagcggagcg ctgccgatgg ggtggcgcat
     1141 gcagtcaatt gtcgcttctt tgacgtcgac ggcggggacg tgctttcctt ccatgacgtc
     1201 ggaaatatgt tcttccggca gtttgacggt ctgtgtcgtt tcaccaatac tgaaagtaaa
     1261 ttcttttaat gccattgcct atcaccactt ctttttttaa aattaatatc aagttgtaac
     1321 ttattatatc atgataaaca cggcatgcaa gattaaagtt aaaacaatcg ggcataacgt
     1381 tggcaaatcg gtcatacgtt tccgatatag acgtggtgca gatgcttttt ggcaacggct
     1441 gctacccggt gaagggtatc gaccggtgtc atcggcgtcg tatcccgata acggggaaag
     1501 tagcgagtca gatggagggg aatatccgga cgaagtcctg ccagccagcg ggcttcatgg
     1561 tctatttggt cttcatcgtc gttttttccg ggaatgatta aggtcgtcac ttcgacgtgg
     1621 ctgacggcag cggcagcggt aatcgtattt aatacgggct gcagggaggc gccgcactgg
     1681 gagcggtaga actcttcgga aaatcctttg aggtcgatgt tccaggcgtc gatatagggg
     1741 agaagccctt cgaggggctt tctttcgata tagccattgc tgaccatgac gttgaacagg
     1801 cctcgtttct taagcagcgt cgctgtttgg attataaatt catagctgag ggtcggttca
     1861 ttataggtat aggccaggcc gatattgccc tgaggtacgt atttttcggc taaggcgacg
     1921 gcctctcccg gtgtcaggtc ttgggacggt gccggctgct gcgaaatctg ccagttctgg
     1981 cagaagggac aggtgaagtt acagccccag gaaccgaggg ataagataag ggatccgcag
     2041 tggaagtgat agaggggctt cttttcgatg ggatctaagg caagactcgt gatttttccg
     2101 tagtttaagg cctgaagggt cgtaccgtca aagcccctcg tttggcagcg gccgcagcga
     2161 cctttctcga tgcggcagtg atgggggcag aggccgcaga cggccttgcc gcttcggtct
     2221 atgtgataaa aggcagcttc catggggata cctacttgta acggtcgacg gtgaagcggt
     2281 ataaatcggg ctgtatctgc ggcgggatgc cggccttttc cttggcgatg ctgatctgct
     2341 gttcgacc
//
LOCUS       DAWDXZ010000284         2340 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8750_length_2340_cov_6.466083, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000284 DAWDXZ010000000
VERSION     DAWDXZ010000284.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8750_length_2340_cov_6.466083"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          697 a          494 c          538 g          611 t
ORIGIN      
        1 caaaacactg catagcatac ggagcctgaa tcaagacatt cgtatcgagc acataagtct
       61 ttgccataaa aatcccacct tttagtctat tttttacagc ataccaaagg aacatataga
      121 acatgtaaag cactggaaaa attcaatgta aaacaattga aatcttcttg actttttttt
      181 acgttagtag tatgctacat acaagatacg agttaccgac gaaggagttc atcatgttaa
      241 aaaaagtatc gtataaagat caaatctatc aatacttaaa agaagcaatt gtaaagggtg
      301 agttattgcc cggtgaagtt tattctgaaa aacaatttgc cgacaagttg aacgtatccc
      361 gcacgccggt acgcgaagct gttttacagc tgaagaatga tgacctcatt gaaatcttca
      421 ataatcgtgg atttggtgta aaagcaacat ctcccgatga cttgcggcaa attttacagg
      481 cccgtttggc tattgaaggt tacgcggtgc tgtgtcttgc caatcacccc catgacaagc
      541 gatggcagga gactgtcact gcattgattg aatgtcaaaa gacagaagcc ggatattctg
      601 atgacgatga atcccattat gaattcatga aggctgatat ccagttccat catttaattg
      661 tagattttcc gcagaatgcc tatttaactc gtatggcgaa tctcatgcgt acgaaaatcg
      721 aattggcgac ggttcattcc cttcgaagta cacatcgcca tgggaaggct tatgaggaac
      781 atcagcaaat cttagatgct atcttgaccg gtgacagcga tagggcggca tctgcctttc
      841 gaaagcatat ggaagagacg gccattgtac taggggcgaa aggctcctta tagaagtacg
      901 gcgcaggatt cctgcgtttt acttttactt taagtagtat gctacgcact actaagtgcg
      961 taattatttt taatgcaagt agtatgttac atggtactta attttcagga ggtaattata
     1021 atgaaaaaaa tcggatttat tggcttaggt attatgggaa aaccgatggt aaagaatttg
     1081 ctgaaagctg gttttgaagt cacagcctac gatatccttc cggctgccgt tcaagaaatg
     1141 gcagaagccg gagccattgc cgggacgtct cccaaacagg cagcacaggg aaaagacgtt
     1201 gtcattacta tggttcccag tggtcctatc gttcgctccc ttttagaagg ggaagacggg
     1261 gtgttggccg gtattcccga aggcgccgtc attgtcgata tgagctcggt tacggctatt
     1321 gaatctcagg aatttgccaa gcttgctgaa agcaagggat gccacttttt agatgcaccg
     1381 gtcagcggcg gtgaaccggg ggccgtcgcc ggcaccttgg ctattatgat tggcggcaaa
     1441 caggaagtcg tcgacaaagt ccatgatgta tttgaagcca tgggttcttc gattaccttg
     1501 attggcccta atggcagcgg ttccattgcg aaactggcaa accaggttat cgtcaatctg
     1561 aatatcgcgg cggtatcgga agcccttatt ctggcaacaa aagccggagc cgatccgaaa
     1621 aaagtgtatg aagccattcg cggcggtttg gccggcagtg ccgttcttga tgccaaagct
     1681 ccgatgatgt acagccgtaa ctttaaaccg ggcggaccga ttcgcatcaa cttaaaagat
     1741 atcaataatg ttatggctac ggccaacggg ttagacttac cgctcatcct gaccagcagc
     1801 ctgcagcaaa tcatgaacag tctgaaatct accggtcatt tgatggacga ccacagcggt
     1861 attgttcaat tctacgaaaa tatttccggc gttattgtta aaacgaacga ggaataagga
     1921 gaaatcatca tgagcatatt aaaaacggaa ctttttgccc ggattcccaa tggcaatcct
     1981 gaacaagtaa aaaaattatt agccgatgaa atgaaagatt tcaataagaa aatcatcgtc
     2041 cttgacgatg atccgacggg ggtacaaacg gttaacggca ttcatgtata tactgattgg
     2101 tccccggaaa gcattgccga cggctttgca gaagataatt cgatattctt tatcttgacc
     2161 aattcacgtg ctttctcagc agctcataca caagaagtcc atcagatcat tgctgaacgg
     2221 attgtagctg aagcgaaaaa aacaggaaaa gacttcatga ttatcagccg cagtgattca
     2281 accctgcgcg gccactatcc cttagaaaca gatacccttc gtaagacctt ggaaggcgaa
//
LOCUS       DAWDXZ010000285         2328 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8785_length_2328_cov_8.371315, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000285 DAWDXZ010000000
VERSION     DAWDXZ010000285.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8785_length_2328_cov_8.371315"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          470 a          584 c          633 g          641 t
ORIGIN      
        1 gtcactgctg tagatgcccg tcttgaccgg aacctgttcg gtcgtgccgt catccttgac
       61 gcgaaggacg taagagccgg aatcattggt cttcaaagcg gcgatgggga cgaccagggc
      121 atcttgtttt tccgatgtcg taatgtcgac gcgggccgtc atgcccagtt tcaagacgtc
      181 gtcaggatca ctgacgtcga gggtgacgta atagtagatg acgctggccg aagagctgga
      241 ggaactcgac gaggacgaag acgacgagct gttcgtatcc caggtgttgc tggtatcagt
      301 cttagaaatc ttcgagacgc gggccgtaaa ggtgcggtct gtataggcgt cgaccgtaaa
      361 ggtcgcttcc tgtccgactt ggacttttcc gatatccgtt tcgtcgactt gggctaagat
      421 ctgtttgctt gacgtatcgg caatgcgcat gatgaccgtc gggttgctgt tgccctgaac
      481 ggccatggtg ccgaccgtct tcggttcgcc gacgacgacg ccgtccatgg gggccgtaat
      541 gaccgtttcg gctacgtctg attcagcttc tgacaaggta ctgagggccg tatcgtagtt
      601 aaacttggcc gtgtccagat cctgttttgt tccggcgccg agctcataca gcttttcagc
      661 tcgttcatat tccaccttgg tattggtgac cttgtactga gcctgatcgc gctgggcttc
      721 ataatcttta ccgtctaaga tggcgacggt ttgaccggcc gtgaccgagt cattttcctt
      781 gaccaagacc tggctgatac gggctgtgat cttcggcgat acttcgacgg aatcgacggg
      841 actgatggtc cccgtggccg atacggtcga cttcagatct ttacggacga cttcagccgt
      901 ttcaacggca gaggcttgtt ttgcctgctg ctgaccttgg tagtaggtat acccgccaaa
      961 agcaagggcg gcgatgacga cagcggcaat ggcgattttc aggcgcttgt ctattctcat
     1021 gatatcactc caaacgtata atacttgaaa tttgctgcca atagtaattt ttagtttata
     1081 atcagtacca gtatagcatg gcactggttg aaggataaag aaaaagccta tagttatcgt
     1141 aaagtgagtt aaaactgtga taaatcttcg cttctccata gggtttttca aaagaatatg
     1201 gtatactgag ataggttatt atttataaca gtatttataa tagaaaggtt tgatactata
     1261 tggtaaatga acgaaagacc tcggcctttt ccaacggctt gctgtggttt ggcgcaggga
     1321 tttccattgc agaaatcttg accggcatgc tcttggcccc cttaggcttt gcccgggcct
     1381 tggaagcgat tatcctcggt catatcatag gcggcatcat catgtacctg gccggcctca
     1441 tcggcggccg gacgggaggc agtgccatgg aaacggtgcg catctccttt ggtacgcagg
     1501 gtgccaagct cttcgccggt ttgaacgtca tccagcttgt cggctggacg gccgtcatgg
     1561 taggcagtgc agctttggct gccaacacca tcgcagccat cggtatgtcc tggtggagta
     1621 tcattatcgg tgctttcatc gccttgtgga tcatcatggg cctcaagaat ctcagcaagg
     1681 tcaatgtcgt cgtcatggca gctttgttta ttttgacgat tatgctcagt atggtcgttt
     1741 tccaaaacgg tacgccaacc gttccggctg gggaacttac cttcggcggt gccgtcgaac
     1801 tggccgtcgc catgcccttg tcgtggcttc cgattatttc agactatacg cgaacggcta
     1861 agaagccggt cttggcaaca gccgccagct gcggtgctta cttcgtggct agttcctgga
     1921 tgttcatcat cggcatgggc gcggctctct ttacggggca ggacgatatt gccgccatca
     1981 tgctccaggc cgggatggga atcgccggta ttctcgtcgt cttgttgtcg acggttacga
     2041 cgactttcct cgatgccttt tctgccggcg tcagcttcca gagcttagct cccaaggcct
     2101 ccgataagat catggctctt gccgcgacgg ttctcggtgt tgtcctggct gtgtttgcag
     2161 ctcctgaaca attccaagat ttcctttacc tcatcggctc ggtctttgca ccgatgacgg
     2221 ccatcctctt ggcagatttc tttattttaa agagagacca tacggcagaa agcgtcaatt
     2281 ggcttaacat cgttttatgg gtcctcggcg tcatcttgta ccgttacc
//
LOCUS       DAWDXZ010000286         2326 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8796_length_2326_cov_6.538529, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000286 DAWDXZ010000000
VERSION     DAWDXZ010000286.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8796_length_2326_cov_6.538529"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1105..1114
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          517 a          664 c          576 g          559 t
ORIGIN      
        1 gaatatgccc acgcccaagt cgcagactgg atgggggacg atacgccgcg aatgatgggg
       61 cgcctgacac tgaatcccgt agctcacatc gatccggtag gactgatgat gctcctcgta
      121 gcccgctttg gctgggcgaa accggtcatg atcaaccccg gcaacttccg caactggcgg
      181 aaaggggaga tctgcgtcgc cttagccggc cccgtatcca acctgatcac gggctttatc
      241 gccctcgtcg tccagctgat tttcgccaag ctgaacctct tcacctcgac ggccctgccc
      301 atggtgctgc agctcatcgt cttgtacaac gtcaacttcg ctatcttcaa catgctgccc
      361 ttgccgcccc tcgacggctc acgggtcctg atgtgctttt tgccgacgga atggtcgtat
      421 aagctggctg gcatcgaacg ctacagcttc ctgatcctca tcgccttgat gatgacccct
      481 gtttttacgt atattttgat tcccctgcag cgcttgatct tcacgttgtt ctcgctgctt
      541 ttggctccct tttggtaagg agggtttatg atgatgtata agaaaataat cgcagccggt
      601 ttattgtgga cggcccttac ggcctcggcc ggcgcttttg actataacca gtccgtcgat
      661 gaaatcgcgg ccaatccctt gacggcctat cagacggtgt acctcggcat gccgagagcc
      721 gattttgacg gcaacttctc ggttttatcg gattggacct tctacggcag caccgtgtcc
      781 ttcacggaaa aagccgaacg gacgtcggtc gtcaagaacg tttccgtcac ggaaggcatt
      841 gaaatcttct ccgctgatac gtcggccaaa ggccgggtcc ttgcctttga caactatttc
      901 aagaccaagg acaaggccac ggccaagtcc atttacaccc gcctcgtagc cactatctat
      961 tccaatatgg aaaacttccc ggtcaaacag agcgataagg aagtggaatg gaaacaagac
     1021 gacgtctcca tcaccgtcgc ctttgacggc caaaaggaca gtgacggcga ctacatcgtc
     1081 tacatccgcc gctacaacaa cgagnnnnnn nnnncaatga acctgagaac gtctataata
     1141 cggtcatagt tgattcaacc tttttacatt tagctttttt tgaagagggt gagttttatt
     1201 aacagtgtga aaacggtatt cctcatgacg gccctggcct gtatcctcat ggctctgggc
     1261 ggcattttcg gcggccgcag cggcgtcatg attatgttta ttatcgccat gggtatgaat
     1321 ttcttctcct attggttcag cgataccatg gtactgcggg cttataaggc cgaacaagtc
     1381 ggagaagatc actatctgta ccgcatcgta gccgatttgg cgcagcgtgc cgatttgcct
     1441 atgcccaaag tctacatcat tccgaccgac gtgcccaacg cctttgcaac cggacgaaat
     1501 ccgtcccacg cagccgtagc ggcaacggca gggatcatga atctcctcga tgaagatgaa
     1561 atccgcggcg ttttggccca tgaaatgagc cacgtccttc accgcgacat cctcatcagt
     1621 accatcgtcg cttcctttgc cagtgccatc gccatgattg ccaatatggc ccagtgggct
     1681 gccatcttcg gcagcggccg cgataatgac ggcgaaggcg gcaatcccat cgccctcatc
     1741 gcaacggctc tcttagcgcc cttagcggcg tcggtcatcc aaatgggtat ctcgcggacg
     1801 cgggaataca tggccgatga agaaggggga cgcatgatcg acgatcccat cgccttggcc
     1861 cgtgccttgg cgaaaatcga caattacgcc cactatcagg tattgcccgg tgccagcaag
     1921 gcaacggccc acatgtgcat catcaatccc ttttccggcg cccgcagcac gctgatgaac
     1981 ttgttcagca ctcatccgcc gacagaagaa cgcattgcac gcctcgaaga cttagacaga
     2041 caattacacg gataaaacct atgcagccgc tctctggaaa ttatttccgg agggcggctt
     2101 tttccgtttc ccggcgtttg catttttttg cagaaacgat ataattaaac cattgcagcc
     2161 acaccgtttt ttttatgaca ggaggggata agggttggca caatatatca tgctgcaggg
     2221 gacgagttcc catgtcggca agagcatcct tacgacggcc ttgtgccgta tttttttaca
     2281 agagggacag cgcgtcgttc cctttaaggc ccagaatatg gccctc
//
LOCUS       DAWDXZ010000287         2320 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8822_length_2320_cov_8.776159, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000287 DAWDXZ010000000
VERSION     DAWDXZ010000287.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8822_length_2320_cov_8.776159"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    671..680
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1697..1706
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          551 a          684 c          611 g          454 t
ORIGIN      
        1 aggctaagat ttcctttgac gccatcaagc gcctttctaa agaaaaagac ggcaaacttg
       61 tcttggttac ggccattacg ccgacgccgg ccggcgaagg caagacctta acgaccattg
      121 gtttggcaca ggctttgaat tacatgggcc ataaggccgt cgtcgccctg cgcgaaccct
      181 ctctgggccc tgtctttggc atcaaaggcg gcgctgccgg cggcggttat tcccaggtcc
      241 tgcccatgga agacatcaac ctccatttta ccggtgacat gcacgccatt acggcggcca
      301 acaaccttct ggcagcggcc atcgacaacc acgtccatca gggcaacgcc cttcgcatcg
      361 acgtgcgccg catcatctgg aagcgggtca tggatatgaa cgaccgggcc cttagaaacc
      421 tcgtcgtcgg catgggcggt aaggtctgcg gcttccctcg ggaagaccac ttcatgatca
      481 ccgtcgcttc ggaaatcatg gctgctttat gcttagcccg tgacctcatg gacctcaaga
      541 agcggatggg ggacatcacc gtcgcttatg acctggacgg aaatttggtt acggcccgtc
      601 agctcggcgt cgaaggggcc atggccatcc tcatgaaaga tgccatcaaa ccgaatctgg
      661 tccagaccat nnnnnnnnnn atggcggtcc ctttgccaac atcgcccacg gctgcaactc
      721 cgtcgtcgcc acgaaactgg ccatgaagat gggcgacatc gccgttacgg aagccggttt
      781 cggtgccgac ctcggcgctg aaaagttcat ggacatcaag tgccgtttcg ccgggatcaa
      841 acccgatgcc gtcgtcatcg tcgctaccgt ccgggccctc aagatgcacg gcggcgtcga
      901 taagaagaac ctccaggaag aaaacgtcga agccttgaag aaaggctttg ccaatcttgc
      961 caaacacatt gaaaacatgc gcctcttcga cgttcccgtc gttgtcggca tcaataaatt
     1021 catcagcgat acggatgcgg aaatccaggc cctgcgcgac ctctgcgccg actacggcgt
     1081 cgaagcagcc ctcaacaact gctgggctga aggcggcaag ggcggcgtcg aaatgggcga
     1141 aaaggtcctc aagatgctgg aacagcccaa gacgccgtac aaaccgatct acgacgtcaa
     1201 cgcctccatt ccggaaaaac tgacggccat cgtccaaaaa gtatacggcg gcgacggcgt
     1261 cgtctttgaa ggcaacgcga aaaaacagat tcaggaactg gaatccttcg gtctcgacca
     1321 catgccgatc tgcgtcgcta agacccaata ttccctgtcg gacaatccgg ccctcctcgg
     1381 tgctcccaaa aactttacgg tcaccgtcaa agacgtccgc gtctgcaccg gtgccggctt
     1441 catcgtctgc cagacgagca atatcatgac catgccgggc ctgccgaagg tcccggcggc
     1501 caaccgcatg gacatcgacg aaaacggcgt cattacaggt ctcttctaag atggattata
     1561 cgaagttcca atcgatgccc ctcgacgagc tcctgaccgt tacggcatcg aaagaaccta
     1621 ttcccggcgg cggcggcatc gctgccatga cggctgcgtc ggcagcagcc ttggtcgaaa
     1681 tggtggccaa cctgacnnnn nnnnnnggta tgaagaaatc gaaaaactca tgatttccat
     1741 tcgcgataag gcccgggtgc tgcggcagcg gtatctcgaa ggcatcgccg aagacgcggc
     1801 ggccttcgac aacgtcatcc gttctgtccg cctgcccaag gatacgccca atcgggcggc
     1861 catcgtccag caggccttta aagatgcggc tacgtcgccc tttgagttgg ggaaggacat
     1921 cttcgccctc ctgcaattag cggaacaggt cgtccgctac ggcaacgcct gggtcatcac
     1981 tgacggggcc attgccgcca tgaacgccag agccgccatg aggtctgcct tctacagcgt
     2041 ccgcatcaat ctgcggtcca tcaaagacga agccttcgta gcccggatga ttgcccagat
     2101 tcgcgaaatt gaagaaaagg ccgtcatggt cgaacagaat gtagaatacg aatatcgtca
     2161 gcgataataa aaacagcata acctgagaga gactgtcgta cccgtgtgcg gcagcctctc
     2221 ttgttatata gggcaagata ccgtataata taaagtatac gaaaggagaa tacaatgttt
     2281 gattttaata tactcgcgat tatcgccggt atccccggcc
//
LOCUS       DAWDXZ010000288         2320 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8821_length_2320_cov_10.676380, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000288 DAWDXZ010000000
VERSION     DAWDXZ010000288.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8821_length_2320_cov_10.676380"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    458..543
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          544 a          532 c          634 g          524 t
ORIGIN      
        1 aaatccccgg agttacatgg actcctttta gaaaaggagt ttgtgccata gggggactga
       61 aacgtttctt ctaaatcgtt ttgacggagg tttgttgacg cgtcggcggg cgcgtaaaat
      121 tgaagatggc atgaaaaaaa acggtaccgg catcggtgaa ggatgtctgg taccgttttt
      181 gatgatggga gtcatgttgt gggagtccga aaaggggcag tgccgtctgt gtcaaaggga
      241 gacatgagcc gtcaaacttc cagccgaatt gggggctcgg gctgacgtct cggaagggga
      301 ctacagggag gcagaaggtt tgaggccctt gtgcgtgctc gggctcgtct gattccttcg
      361 gtccttgaaa atgacggcct ctttgtgagg atgaagaaaa tgagaaaaat tcctcttttc
      421 tcgataccat aaggtatctg actatatgag tttacaannn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnntgttatc gattttacat tcacattttt tcgagttata tttatttaga cgaaatattt
      601 ttggtaacat aatggcggac ctgtaggttt ttccccctgt cttacatggc ttcgatgatc
      661 ggattatatg gagaggccat cccttggtga aacggaataa aaaagagcga cttcgtattc
      721 tatgaagacc ggcgtcgtga tacaatagca ggagatacgt actatggacg cggacgtacg
      781 ggtccgcgcg aaaaggagca tgatcatgaa gatagagtat atgttggcca atcccagcgg
      841 caataagacg attttggtgc tgacgccggt ccctaaggaa gaacacagta ccctggcggc
      901 ggctttactg gcaaggcctg acatcgacgc cgagcaggtc ggctatgtga cctgtatgga
      961 aggcaaaccc cttcgcgtcg atatgatggg cggcgaattt tgcggcaatg cttcgcgggc
     1021 agcggcggcc tacgccttgt cctgcgaagg gaaggatcag ggcgtctacg gcgtatcctg
     1081 ttctggctgc gacgaaatcc tgccggtcaa ggtcaatcat acgggaggcc gggactatga
     1141 ggcctttatc gagatgccct atcctgacga cgtcagcggt attttggtag acgtcggcgg
     1201 ggtgccggtt cgtttcttcc gcgtcgacct gccggggatt acgcactttg tccacttcgt
     1261 agagaccttt gaaggccttg ataaggacgt ctattggcag gccttgaaag actacgtcga
     1321 cggcgaagat ttcccggcgt acggcctcat cctctgcgac accaaaacgc aggagatgat
     1381 tcctgccgtc tacgtcagcc ggacggatac cctctactgg gaaaacagct gcggcagcgg
     1441 atcggcagcc gtcgcagcag cgcttgcctg tacgacgcgc aaacgggtgg ccgcttatat
     1501 gaagcagccc ggcggcacca ttgccattgc agccagtgtc gatgacgacg gcaatctgca
     1561 gcagattttt atgggcggtt cggtaaaact aggtcctcgt caaagcctgg aatgagggcg
     1621 cagccttgtc ttttgccatg gggcaggctt atgccgaaaa aatacttgac tatttgactt
     1681 tttgcggtat gatagtgatg tgaaggtacc tcagatgcca ctgacggtac ggaaaacgga
     1741 tacaaaggag tgtttttacc atggcaaaag aaacccatga atttcaagct gaaacgaagc
     1801 agttattgga cctgatggtc cactctattt atacaaatca tgagattttc ctgcgggaac
     1861 tcatttctaa cgcctccgat gcgatcgata agctccatta tcagtctctc actaacggcg
     1921 atctcctcga aggggacaca ggctttgaaa tccacctgac gcccgatgcc gacggccata
     1981 ccctgaccat ttccgataac ggcctgggta tggacaagca ggatttgatg gacaacctcg
     2041 gcaccattgc ccagtcgggg acgaaagcct tccttgaaaa gctgaaggaa tcccaggacg
     2101 gcggcgatac gaataaggac ctcatcggcc agttcggcgt cggcttctat tcggccttca
     2161 tggtcgccga tacggttacg gtcgtcaccc gtaaggccgg cgaagctcag gcttacaagt
     2221 gggaatcgac gggaagcggc gaatacaccc tcgaagaatg ccagaaaccg aagcacggga
     2281 cgacgatcat cctccatttg aacagtgact tctacggcga
//
LOCUS       DAWDXZ010000289         2320 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8823_length_2320_cov_6.917439, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000289 DAWDXZ010000000
VERSION     DAWDXZ010000289.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8823_length_2320_cov_6.917439"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1026..1035
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          547 a          592 c          617 g          554 t
ORIGIN      
        1 ataggaggtt ttagaatgtc taaatctgaa tacaccttgg ttttgccggc ttttaccatc
       61 gggatacatg cctatgatgc cattggtaag atcaccaaac actttgggaa aaaagtcgtc
      121 atcatcggcg gtaagacggc tctgtccaag gctaaacagc cgctcctcga tgccattgcc
      181 aaaacggatc tcgaagtcct cgacgtgttg tggtatggcg acgatgccac ctttgaacac
      241 gtcgatcgcc tgctgaaaga aaaagccgtt caagaggcag atctcgtttt cggtgtcggc
      301 ggcggccggg ccatcgatac ggggaaggtc gttgccgata aggccgataa agccttcttt
      361 gctttcccga ccctggcgtc taactgcgct ccgtcgacgg ctatttccgt catgtacaaa
      421 gcagataagt ccttcgccgg ctattattac ctcgatcagc cgccggttca caccttcatc
      481 aatatggctg tcattgctga ttctcccttt aaattcttct gggccggcat tggcgatgct
      541 atgagtaagg aatgtgaatc ggaactggcc agccgggctg cagaacactt ccataccgtt
      601 ctcttgggcc gggctatcgg aaagacttgt acggaaccgc ttctccagta tggggaaaag
      661 gcactggcag acttcaaggc caaccagatt acgtatgaac tgcagcaggt cgtcttagac
      721 atcattatca gcacgggcct cgtttccaac tgcacgacag gcggtgaaga atactattat
      781 aattcgtcct tggcgcacat gttctacaat gcctcgacgg tccttcccca ggttgtcgaa
      841 ggccatcttc acggcgaaac tgtttccttc ggtgtcttag tcctcttgac ctacgataaa
      901 cagtttgaac tgcgggataa ggtcgctgct ttctataaaa aatgcaatta cccgacgacc
      961 ctggcagcct tagacatcaa ggccgatgaa atcgacgccg tcgtcgatgc cgccccggcc
     1021 ctgcgnnnnn nnnnngcgtg aatggacctg cgttcccacg ccgatgacca aggatgcttt
     1081 taaacaggct attctcgata ccgatgaatt cggtaagtca ttataagcaa catttcgtat
     1141 atgcacatcg tttgtcatag gagatattcc ggcatggtga gacgatgact gtcttttatg
     1201 gaggaagtat ggatcctgta ttagaacaaa ttattaaggc cattgaaccg atggatctgg
     1261 ctaaggctgc ggagtgctac cagcgcttgg ccagcctgac aattcgccag gacagcaaat
     1321 tatcgtatct ggcaggccgt attgccggcg tgcagcagac ccttcatccg gaaacgctgc
     1381 agaaggccat tgcaatcttt gctgccgacc atgccgtcga cggcggggaa aataagaccc
     1441 agggcaagac cagtaaggcc gatgctttag aaattgcagg aggccgcggg gctatcaata
     1501 aggtggctca ccgtatcgga gccggtgtca tgctcctcga catgggcctt gaagatgact
     1561 tggccgaatc gcagggggtc cagaatctga aagtcatgca cggcagccat ttttgggctc
     1621 gccgggacgc catgagtgaa gatgaaatga tggatgccct gttcagcggt ctgcagcttg
     1681 gcgagaacct ggctgaagaa ggatatacgg ccgtcggcct cggcaatatc ggcgaacggg
     1741 atctcctcac agcttttatc ataacggcgg cctttttccg cggtcaattg gaagaccttc
     1801 cggcgtctat gacgtccggc aaaaagttgg attcgctgaa acgcgtcatg gaggaaatga
     1861 atttcgaccc ggaaaatcct ttggatttgc tgcgccgcgc cggatcgccc gacattgcgg
     1921 ccatggtagg gttcatttta tcggcagctc ggtgtaaaat gctcgtcgtc tttgacaatg
     1981 ccgtgaccgg ggcggccgtc ctcttggctc gcgccttgtg cccgacggtt gaggactatg
     2041 tttgcccgtc ggctcaatac caggaaccgg tacatcagat gcagatgaag aaattaggac
     2101 tgaaaccctt cgttgaactc gagcagaatc tcgaccaggg aatgggctca gccatggggc
     2161 tgaccatgct cgatgccgct gtccaaatga tgaacgataa tttgaagtaa ggagtcccac
     2221 gatgaaaaca ctacatctcg actgtttttc cggtatcagc ggcaatatgt tcgtcggcat
     2281 gctcatccag gcaggggtcc catttgaagc ttttcaaaag
//
LOCUS       DAWDXZ010000290         2307 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8891_length_2307_cov_6.768206, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000290 DAWDXZ010000000
VERSION     DAWDXZ010000290.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8891_length_2307_cov_6.768206"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1840..1849
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          487 a          632 c          644 g          534 t
ORIGIN      
        1 catcgtagcg gacgatgccc atcaatcgcc cgtcagggcc atagacacgc agcacgtctc
       61 catcggtgag gttcggcccg gcaaagggca caggccgccc ctgaaggaga aaatcacact
      121 gcttggtcga gcagttagcc cgccttatat cgtgaatggc catgtcggcc ggaaggacat
      181 aagacgcagg atcttgttcc aacatttcta aggtcgctgc ttcttcaatg gtaaacagac
      241 cggcccgggt tcggacaaga taggtcatgc aggcgcaggt cccaagggcc gtccccaaat
      301 cgcggatgag ggatcggaca taggttccct tagagcaagt cacggcaaag gtaccgcgcc
      361 ggccgtcaaa ggacaggagt tccagactgt ggattgtcac ctgccgcaca ggcaaggtga
      421 aatcccggtc ctggcgggcc aatttatagg ccttgacgcc gttgacgtta atggccgaat
      481 acttcgacgg cgcttgttcg atatcgccgc agaaagagga caggacgcgg cggacatctg
      541 cttcggaaag atccggaacc ggcatccgtt cgacgaccgt ccccgtactg tcttcggtat
      601 cggttgccag ccccaggacg aattcggcat ggtaggactt gacgtcgctg tcgccgtatt
      661 cgatgaggcg cgtcgcctgc ccggcataga ccggcaggac accggctgcc aaggggtcga
      721 gggtaccggc gtggcccatc ttcttcatgc cgtaaatgcg ccgcaggcag ccgatgacgt
      781 cgtggctcga cattcccgtc ggcttcagta cattaatgat tccatgtatc ataagcgctt
      841 ttcaatttcc tttacgatga tcgccttagc ttcatctaaa ggcttttcga tggtacagcc
      901 ggcagcccga atgtgcccgc cgccgccgaa agaagcggcc acagcattga cgtcgatgcc
      961 tttcgagcgc aggctgaccc gcgtttcttc agggccgatg tattttaccg taaaggccac
     1021 ttcagcgccc ttgatgcggc gggcatagtc gatatagatg cccgtgtgct cgccgatgac
     1081 ggaaaggagt tccggcgtgt aggacaggca ggaaatgcgg ccgtcggcaa agagttccag
     1141 ctgatcgaga accttcgaca aggcccggag cttgttgagt gaccgagcgt cgagatggtc
     1201 tgaaatgacg tcgggctgcg caccgttgtc gacgaggatg gctgccatgc gcagggtatg
     1261 gcccgtcgtg ttgttaaact tgaagaagcc gcagtctgtg gcgatggccg tatagagggc
     1321 atcggctatg gccggagacc acgtgatatg ccactggtca aagaggtagg tcaggatttc
     1381 tcccgtcgcc gcatagtcgg gcttaaaata ttcccactgg gcaaaatgct cattcgagac
     1441 gtgatgatcg aggttcaaaa ccttgccgca gacgaggtca gctgtcttac cgatccgtcc
     1501 taaacctgtc gcatcgagga cgaccgtcag ccacgaggca tcggttttga cctggtcggg
     1561 ccggcaaata gccgacaagc catcgaggaa gttatagcgg tcgtcaatgt catcgtcgat
     1621 gaccatgacg gcatccttcc cttctgattt caaccaatgc cacaaagcca agacgctccc
     1681 taaggcatcg ccgtcaggac tgacatgggc cgtcaccaaa atgtggtcat agtggtgaag
     1741 gagggtatag atttcctggg cggagcaatt catgtcatca gtccttcttt tcattgtggt
     1801 gcagaatcta atcgattttc atcccatagt catgggacgn nnnnnnnnnc ttcctgttta
     1861 ataaattcct ggatttttcg tactcgtaat tctcccatcg aatgtcccct cctttattcg
     1921 ggttcgactt cgaccatttc gtaggcttcg atttcgtcgc cttccttgat gtcgcggtac
     1981 ttttcaaggg taacgccgca ttcgtagccc gaagcgactt ccttgacgtc atctttaaag
     2041 cggcgcaggc cgtcgatttc gccttcgtga acgacgatgc cgttacggat caggcggatc
     2101 ttagacgtgc tggtgatctt cccttccttg acataggagc cgccgacgat gaccttcggc
     2161 gtcgtaatga ctttacggac ttcggcacgg ccgataatct tttcttcgta tttcttttcg
     2221 agcatgccct tgatagccgc ttcgacgtcg ttaatggcgt cgtaaatgac gcggtacatc
     2281 cgcatgtcga ccttttcgtg ttcggcc
//
LOCUS       DAWDXZ010000291         2305 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8903_length_2305_cov_5.571556, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000291 DAWDXZ010000000
VERSION     DAWDXZ010000291.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8903_length_2305_cov_5.571556"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          654 a          603 c          533 g          515 t
ORIGIN      
        1 gctcttttaa attcttcata aggcgtgtca ttggaagaaa agacgatatc atggccggta
       61 atgccaactt tgtcacatag ttccaattct gccatggagc tgcagtcagc accgacgcct
      121 tctttctgca agagacgcat gagatacgga ttcggtgcag ccttaacggc aaaatactct
      181 ttgaaatcat gggcccaatc aaaagccttt atcaattttc gaacgttatc gacaatggct
      241 ttttcatcat aaatatgaaa aggtgtcggg tacttggcaa tgatagaacg aatctgttct
      301 tcattaaagg ggacagtttt cttgttcatc tgattcagct ccaatttctt ataatacagc
      361 aagatgcata atctaaacta caaaacaaac ttgcatttca ttgtttctct acaatgatta
      421 ttatataata caaagagata atttgtgaaa tagattattt caattcattc cattcgataa
      481 tcaaattgca agaaaaggag gtccggctat ggatacgacg acactccgga cattcattgc
      541 cctggcacaa ataaagaact ttacgaaaac ggcgcagcag ctcttcgtcg cccagtcgac
      601 ggtaaccaac cgcatccgcg acttggagat ggaactgggc gtccccctct tcatccgcag
      661 ccacaaacag gtcgacctga cggcgtcggg tcaaaaattc ctcgattatg cccaacgctt
      721 cgtctctctc gaactgacgg cccttcagga tatccagtcc tcgccgacgt acgcccaaaa
      781 agtaaacatc ggtacgacca atacgattta cgaaagccac cttcagcgcc gcatccgcag
      841 ctacttaaag aagcagaaag acgtctctct ccatgtcact atcagccata cggccgacat
      901 gatgcgccag ctccaagaag ggaccctcga cgccatcttt tccttcagtg ctatgttcaa
      961 aagcggcttc gtctgccagc cctaccggac cgactcattg gtcctggtct gtaaggaatc
     1021 cgatacctcg tacgccaagg gcatctataa ggatgatctg caaaaaatcg attacttatt
     1081 ctgtaacttc gccctccaag gcgtcggcct ctacatacag gacctctttc cgcgccatca
     1141 ccgcttcccc ttagaaatcg acaacagcac taagctcccg cagtacgtcg ccgaaggcat
     1201 cggctatacc tttttgccga aaagcctcat cgaagacgac ttagcctcca agcggctgcg
     1261 ggccattccc ctcctcgatt tcgaaccgcc aaaggtcgac agctactaca tcacccgcag
     1321 tccgtcgacg gtaccggcgg ccttagaaga agagctcttg gccgatcggt catatcgttt
     1381 ccaagacgac gatgagcgtc gacatgacga cgatggaaat gaggatatat aacgaactga
     1441 tgcaggccgc cacttcgatc tttaacttca atttcgccgt atagtagcag gccatgccgg
     1501 caaccggggc aaagaccagg accatgagga cccgcttgat gtccatggaa tagtcgaggc
     1561 cgacataaat aaaggcagca aagacgacag agatcaatag gcgcagacct acatccgtat
     1621 agatgaggtg caggtattcc ttctttaagg acaagcccag ggcaacgccg atcatcagca
     1681 tcgacaagaa ggtattggcc gaagccggca ccttgaccca gtcaatgacc acttggggca
     1741 gagagaggcc ccccaaagcc aggataagca tcaggatgta ggacaggacc ggcagggatg
     1801 aaaagatcgt cttggccaag atataaaaga tattatcccc ttttgcccca cggacgtagc
     1861 gggccaggcc ataaccgccg ccgagacaca tgagggagtt gccggcgtca aagatgcaga
     1921 cgatgaggac cgccgcactg tcgaagaaat acgatacgaa gggcagggca aaattgccga
     1981 tattatagcc gttcatattg agcatataaa aggccttttc gcttttcgtc cgcccgacac
     2041 catagccaag gccgaccata agcaggttga agacgatggc caggaggctg atgaaaaggt
     2101 aatgcacctc gaagtggatg ccgttcaggt tggcaatgat gaccgacggc agggttatat
     2161 taaagacaat cgtcgatatg gtatgaaaag tgctgcgccc aaagaggcgc agctgcttta
     2221 aaatatatcc gcaaagaatg aacgacacga gaatggccgc ttttatgatc gtctgttcca
     2281 tcgcttacgg tttgacgatg cgatt
//
LOCUS       DAWDXZ010000292         2301 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8920_length_2301_cov_9.207480, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000292 DAWDXZ010000000
VERSION     DAWDXZ010000292.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8920_length_2301_cov_9.207480"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    369..378
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1595..1604
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          492 a          575 c          649 g          565 t
ORIGIN      
        1 tggcgcagac accgtaacgg ctgtggcagg taaggacgct gcggatccgg acggtatcgt
       61 aatatttagc aatttctttc gcctggtcgg ccatgatttc ctcattgagg gctacgatga
      121 cggcacccgt ttccgggttg accagggttt cagcagccgt acggccttcg atccgttctt
      181 caagcggttc gatgacgccg ttgccttcga cgatggccgt gacttctacg ccgcggatat
      241 cgttgttgcg ggctttgact tctttgacat cactgttcag gatgcgatcg atggcatctt
      301 ccgtaagggg cgtgcccttc gcgcagattt caatgccatt ggagtcgatg acggcttctt
      361 cgatggtcnn nnnnnnnntc gagttgaaag gatccatgtt atccatcatg acttccgtaa
      421 catcatcggt cgctttttcc caaatagcac aggttttgcg ataccgttca tcttccgtaa
      481 tgagaccgcg ctgatacatc ttggatacgt tctgtacctg tttgtcagca gcgccgagga
      541 tatcccattt ttcttccgga atctgaatgt cggaaatggc gatggacata ccggcctgac
      601 gagcgtagtg gtagccgata gccttaatat tatcaagaag ttctgccgtc ttggtgttgc
      661 caaagaggtg gaagcaggca tcgacgagtt tgccgatttc cttcttgctc atgaggacgc
      721 cgaggcagtc gacgtcgaca tattctttac cttctttgaa agcagcgaca gcatctttat
      781 cgattttatg tttcggaagc gggcgaacgg ccatgaggtc gacaccaaaa gcttctttca
      841 actgggagaa accatagtcc aggatatgac cgtcggcatc tttcactttg atgaagtgcg
      901 tccagtcgat aacgccgttt tcataggcaa atacgacatc ctgaggatta tcgaagacca
      961 taaccttatc cgtatggaag aggcgcagcg gttcgtagat ctggtagttg tacaagagtt
     1021 tgcctgccgt cgtttcaatg aggccgtggc ccgggatgcg gactttgatc aaggcttcaa
     1081 tgtcgatgac tttggcatcg taagcaagca tgacttcatc gtaattggag aagatcttgc
     1141 cttcgccttt cgcagacggt ttaaccaagg tcaggtagta ggcaccgaga accatgtcct
     1201 gtgtaggaac agcaaccggc ttgccgtctt tcggagccag gatgttgtgc gacgacaaca
     1261 tgagcgtccg agcttcggac tgggcttcta ccgacagcgg cagatggcag gccatctggt
     1321 caccgtcgaa gtcggcgttg aaggccgtgc agaccagcgg atgaagctta atggcccgac
     1381 cttcccaaag gaccggttca aaggcctgga tgcccaaacg gtggagtgtc ggggcacggt
     1441 tcaagagaac cggatgttcc ttgatgacgt cttcgaggac gtcccagact tcattgctga
     1501 cgcgttcgac ttttttcttg gcgtttttaa tgttggtggc aatttctttt tcgacgagct
     1561 tcttcatgac gaagggcttg aagagttcca gtgcnnnnnn nnnncccgta acggggcggc
     1621 cgcgacggcc attgtcgatg agggcatcga cggcttcctg aagcatgcgt ttttcattgc
     1681 ggacgatgat gtccggcgca cccaattcaa ggaggcggct caagcggttg tttcggttga
     1741 tgacccggcg atagaggtcg ttcaggtcag acgttgcaaa acggccgccg tcaagctgga
     1801 ccatggggcg cagttccggc ggaatgaccg gaatgacgtc catgaccatc cattccggtt
     1861 tattgccgga atgacggaag gcttcgacga cttccaggcg gcggatcgcg cggacgcggc
     1921 gctggcccga agagtcgcgg agttcatcgc gcagctcttt atcgagctgg tcgaggtcga
     1981 gcccctggag gagctcttta atcgcagcgg cacccatgcc gacgcggaat ttatcgccgt
     2041 acaagtcgag atactggcga tattccgttt ccgtcatcag ctgctgcttc tggagagacg
     2101 tatcgcccgg atcgaggaca atgtacgaag caaagtataa gactttttcc agcgaacggg
     2161 gcgagatatc gagaatcaag cccatccggc tgggaatgcc tttaaaatac caaatatgag
     2221 aaacaggtgt agccagttta atatggccca tccgttcacg acggactttg gcgcgcgtga
     2281 cttcgacgcc gcagcgatcg c
//
LOCUS       DAWDXZ010000293         2300 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8922_length_2300_cov_6.226726, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000293 DAWDXZ010000000
VERSION     DAWDXZ010000293.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8922_length_2300_cov_6.226726"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          509 a          620 c          632 g          539 t
ORIGIN      
        1 atgaattcat ggctgtgatt gccgccgatc tgaccgctgt cggcttcaac ctgtttgaat
       61 tccagaccgc agcgggtaaa gacgcgggta taggcgtcgt attcgtcttg ataactctta
      121 tggagccctt cttcgtcgac gtcaaaagaa taaccgtctt tcatgacgaa ttcccggctg
      181 cgcatgaggc cgaaacgcgg gcgaatttca tcgcggtatt tattctgcat ctgccacagc
      241 gtaaccggca tctgcttgta cgaacgcagt tcgttgcgca ccagtgtcgt gatcaattct
      301 tcgtgagtcg gcccgagaca gaactgaccg ccgtgacggt cttccagttt gaacatttcc
      361 ggaccatagg cattccaccg ccccgattcg tgccagattt cagccggctg gacgatgggc
      421 atcatgattt cctgagcgcc gatattattc atttcttcgc ggataatggc ttcgattttg
      481 agttcgacgc ggcggccaag aggcaggaat gagtataaac cgccgccact tttacgaatg
      541 aggccggctt tcagcatgta ctgatggctg gagatttcgg cgtcggccgg aatttcccgt
      601 agggtcgggc tgtacagttt ggatgctaac atgagttaag actcctttgc gattgatttt
      661 tctatttctt ccaaaagagc agcgacgagc tgatcttccg gtgccgtacg aaggaccttg
      721 ccatgggcaa agacgatacc gctccccttt ccgccggcaa tgccgaaatc ggcttctttc
      781 gcctctcccg ggccgtttac gacgcagccc atgacggcga ccttaatggg aacggtcaag
      841 tgggaaatgg ccgcttcgac ttctttggca agctcgatga gctgtacctg cgtacggccg
      901 caggtcggac aggaaatgag ggtcggtccg aaacgacgca gtccgaggga gcttaaaatg
      961 gtctgggcga ccttgatttc ttccgccgga tcatccgtaa gggatacgcg aatcgtatcc
     1021 ccgatacctt ggtataataa agtcccgata ccgatggcgg acttgatgct tccgtcccgg
     1081 acgaggccgg cttcagtgac gcctaaatga aaggcatagg gcactttatc ggccagttcc
     1141 tgatacgatt caatcatgag cggcacgtcg gaagccttga gggaaatgac catatttcgg
     1201 taatcgaggc gctctaaaat acgaatatgg cgcaaggctc cttctaccat ggccgctgcc
     1261 gtcggatggc cgccatgggc cttcaggata tcggccggaa gggatcccgt attgatgccg
     1321 atgcggatgg gaatttcctt gggccttact ttttcgacga cgcggacgac gttgtcatcg
     1381 gcgccgatat tgccgggatt gatgcgcaag gcctggacac cgctgtcaac ggcagccagg
     1441 gccaatcgat aatcaaagtg gatgtcggct acgataggaa tatcgacggc ctctacgacg
     1501 gctgacagcc ctttggccgc ttccatatcg gggacggcaa agcgcaggat ttcagcacca
     1561 taagaggcca gccttctggc atcggcaatg gcctctgcgg tatgtgccgg gcttaccgtc
     1621 gacatggtct gaatcgagat aggcgcactg ccgccaatgg taacgtttcc ggcctgaacc
     1681 ggaatcgatt gtctgcgttt catcgatgac ctccctttct aaaaacggct gatatcctgg
     1741 aacatggcaa agaggaacag agatcctaag agtaccatcc cgacaacctg aatgtagtac
     1801 agggctttcc ccggcatgcg ccggcgcata atcccttcga gaatcaacaa gatgatatag
     1861 ccgccgtcga ggagcggtac gggcagaaga tttaaaatcc ccaggttgat gctgaggaag
     1921 gccgtaaaca tgagcaggtt agaaaatccc gaggccgcga cttgtcctgc aagctgggca
     1981 acgccgatgg gacctgccac ttccgccttt tcctgaccgg ttatcatctg ataaattcca
     2041 accagcatga gccggcagat ttcccccgtc cgcttgactg ccatgatgcc cgaatcaagg
     2101 aatccgtggc gttcttccgt ggtcataggc atgacgccga gcatgacttt ttgagacgcg
     2161 ctgtcgactt ccggtatcat ttcgacatcc tgacgcttcc cgtcccgctc gatttgaacg
     2221 gtgacgacgg cgtgactgta aggtgctacg gcttccgaaa tatcgctcca ttgggaaatg
     2281 tctttattgc cgatgcgaac
//
LOCUS       DAWDXZ010000294         2299 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8927_length_2299_cov_8.391711, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000294 DAWDXZ010000000
VERSION     DAWDXZ010000294.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8927_length_2299_cov_8.391711"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          521 a          680 c          582 g          516 t
ORIGIN      
        1 gtacagcatc ctcggcggcg tcttaggcat catttgtaat ctatttctct tcgtcagcaa
       61 atttctcgtc ggcatggcca gcaacagtat cgggattttg tccgatgcct tcaataacct
      121 gaccgacatg ggttcctctt tcatttccat catcagtgcc aaactgagta accgtccgcc
      181 cgataaagaa catcccttcg gccacggccg ctttgaatac ctggcatcgc ttaccattgc
      241 catcatcatc ctcgtcgtcg gctataagct ctgcgaaacg tcgattggaa agttcttcga
      301 gccggaagcc atggaattct cctactggag cttagggatt ctcgtcatct ccattgccgt
      361 caaggtctgg atgtgcgtct acaaccgtta tatcggaaag aagataaatt ccagcgtcaa
      421 tgaagcgacc gccgccgaca gcatcaccga tgccatcgcg acgaccggcg tcttagtcac
      481 gaccatcgcc cagcagttta cgaccctccc cgtcgatgca gcggccggca cagccatcgg
      541 tctcatgatc atgtacgccg gctacggcat tgccaaagac gtcatcaata ttctcctcgg
      601 caaggcgccg gatccggagc tcatcgacgg catcgtagaa agcgccttaa agtgccaata
      661 cgtcgtcggc gtccacaaca tccgcatcca cgactacggc ccgggtcgga tgttcggcac
      721 gattcacgcc gaagtcccgg acacggccaa tctggccgac gtccacgccg ccctcgacgt
      781 attggaagac gaattaaagg accgctacca aatggacctc aacatccaca tggatccctt
      841 gtgtaccgat cccgataaaa tcgacgccat ccgccacgtg ttggaccaca tcgtccacga
      901 aaaatacccg cgctaccata cggaccacct gcgcatgacc tccggtaacg cccgcttgaa
      961 catcatctgc gacatccacg tcccgcctga cgaatataat aaagaaaaca tcgcccgcat
     1021 ccgccgggaa atcaacgcct gcatgagaaa gtacaacgac aagtaccgcg tcgtcctggc
     1081 ccagatcatc gccgacccgc aataaaaaag gtgaagaagg aattccttct tcacctttta
     1141 tttatttaac gactaaagac cgcggttcga gcatggcctg caggggctga cgcaattctt
     1201 ccgtcgtttc gacccaatac tgttcgtcgg tcagaatcgt cttgcgactg cccatgaggt
     1261 ggaaataaac tttgacctgg cccggatgtt gtttcagaac cttgccgata cggccggcaa
     1321 ggtcagtccg ttcaaggtgg gccggaatct tgatgaagag tttcgttccc ataccgggat
     1381 gccagtagtc tccggcctct ctagccgcga cagccgatac gaccggtgtc ggctgcgggt
     1441 ctgaggccgc cgtttcctgg ccgaggggcc gcacttcttc ggctaaaatc tggatgctgt
     1501 cgtcgttgat atcggctcgg ccgcgaatag acacggccat atcgacggcg acgaactgct
     1561 gatagcggcc gtaggtcttg gggaatacga cgaccgacac agcgtgggta aagtcttcga
     1621 gggtgatgat ggccatctgc tcattgcgct tggtcatgac ctgctttttc gccgtaatca
     1681 gaccgccgac acgaagcatc ttaccatcat actggcgccc gtcttcccca tagagcagcg
     1741 ataggggact aagcttatcc atgatttctt gatacttgct caaagggtgg ccgctgaaat
     1801 agaaaccgtc atactctttt tccatggcca gccgcatttc aatgggatat tcctcgatat
     1861 cggggtattc gaggtcggtc atgacctctt ccgtatcgtc aaagagaccg atttggcccg
     1921 acgcatagtc tttttgactg agggacccca tggccacagc ctgatcgatg acggccatga
     1981 gctgggaccg cttcttgccg aaggagtcca tggcgccaca tttaatcagg ctttctaggg
     2041 ccctcttatt gactaagtga tagtcgacgc gactgcagaa gtcgccgagg gacgaaaacg
     2101 gaccgccttc ttccctggct ttcacgatgg catcgatggc gttgccgccg acgctcttga
     2161 caccggacag accgaagcgg atggctccgt cctgaacgga aaacatggag acgctgtcgt
     2221 tgacatccgg cgataaaacc ttgacgccgt ggcggcggca ctcggcgacg tagtagctga
     2281 ttttgctgct gtcgttgat
//
LOCUS       DAWDXZ010000295         2295 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8943_length_2295_cov_10.868750, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000295 DAWDXZ010000000
VERSION     DAWDXZ010000295.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8943_length_2295_cov_10.868750"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    816..825
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1777..1786
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          550 a          690 c          648 g          387 t
ORIGIN      
        1 cagcgggtca agctggcgac ggaactgtct aagcgcagca ccggccggac cctgtacatc
       61 ctcgatgaac cgacgacggg cctccacgca gccgacatcc ataaattgat ggacgtcctg
      121 cagatgctcg tcgacggcgg cgatacggtc gtcgtcatcg aacacaactt agacgtcatc
      181 aagacggcgg actacctcgt cgacctcgga ccggaaggcg gcgacggcgg cggtaccgtc
      241 gtggccacgg ggacgccgga agacatctgc aaggtcccgg cctcctatac gggtcaattc
      301 ttgaagcccg tcctggaacg gacgagggcc tttatggaac agggagaagg gaggtaagcc
      361 atggctgtca gtgaagccgt acgcaagaaa gtacagaacc tgcccggcca gcccggggtc
      421 tacctgtgga aggacgagaa cgggaagatc atctacgtcg gcaaagctgt aaatctgcgc
      481 aaccgcgtga ccagctacat ccgtcacgac gtcaaccgct cgcccaaggt ggccgccatg
      541 atcagccatg ctgccgacct ggagaccatc gtcgtcgcca ccgagatgga agccctgatc
      601 ttagaaaaca acctcatcaa gaagcatcat ccccgctaca acatcatgct ccgcgacgac
      661 aagacctacc cctatatcaa ggtcaccgtc caggaagact atccgcggat cttcatgacc
      721 cgccgcgtca gtcgtgacgg cgcccgctat ttcggcccct ttgccgactc gacggcggtc
      781 caccgggtct tgaaactgat gcagcgggcc taccgnnnnn nnnnnctgca gtaccacttg
      841 caccactgcg atgcgccctg cgtccactac attaccaagg ccgactacaa cgagagggtc
      901 gaccaggccg tggccgtcct cgaaggtcgg gacaagggca tcgtccggac cttgcaggat
      961 aagatggctg aagcggcgga agccatggag tttgaaaagg ccgccatgta tcgcgaccaa
     1021 ttgaaggccg tcgctgtcat tcaggaacag cagaatatcg tcaacaccga cggcggcgac
     1081 atggacgtca tcggcctggc ccgtcaggcc ggtcagacct gcgtccagat ttacaccgtc
     1141 cgcatgggga aactcatggg aagggaaacc ttctcgttgg acaacagcag tgacgaagaa
     1201 gaaggggccc tgatgaatgc cgtcctcgac cagtattaca gcgacggtac cttcgttccg
     1261 acagaagtcg tcgtcccggc tatggaagac ggggaaagct gtgaacgccg cctgagcgaa
     1321 cagaagggca agaaggtcag cgtcatcgtc ccccagcggg gaacgaagaa acagctcttg
     1381 gctatggcct gtgaaaacgc cgccgccctc ttggaacaga agcgcctcca gtggcagcac
     1441 gacgtggaca agacgacggg agccgtcgaa ggcttggcgc ggatcctcaa tctgccctat
     1501 cttcccgaac ggatggaatg cttcgatatt tcccataccc agggaatcga gaccgttgcc
     1561 tccatggtcg tctttgaaca cggccagccg gccaagaagg aataccgccg ctttaaattg
     1621 aagaccgttc agggcaaacc ggacgacttc aagtccatgg ccgaaatcat gggccgccgc
     1681 tacggcgaaa aagactggcc cgtgccggac ctcatcatca tcgacggcgg caagggccag
     1741 ctccacgccg cccttcccat catccgcgat gccggcnnnn nnnnnncttg gccaagcgca
     1801 tcgaagaagt cttcgtcgaa ggcagcagcg agtccatcat cttaagccat cacacgccgg
     1861 aactgcagct cctccagtcc atccgcgacg aagcccaccg ctttgccatt acctatcacc
     1921 ggaatctgcg cggcaagcgc agtctggtgt cggtattgga ccacgtcgaa ggcatcggcc
     1981 ccaagcgccg caaggccctg tggacggcct ttaagaccct ggccgatatg aaggcggcgt
     2041 ccatcgaaga actggctgcc gtgccgggca tgaaccgtcg tgccgctgaa aatatctatg
     2101 actttttccg cttagggagc gatgaaaagc ggaaaattgt ccaataaata tggtataata
     2161 aagaaacaga aaaaataaag aggagggatt ccttatgata aacaagaaat tgctcaccct
     2221 tttgtgcgga gcctggctca tgggggctgt cgccatggca gcctcgccgg ccgtcatcga
     2281 agacgcaccg aaggc
//
LOCUS       DAWDXZ010000296         2285 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_8983_length_2285_cov_9.386099, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000296 DAWDXZ010000000
VERSION     DAWDXZ010000296.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8983_length_2285_cov_9.386099"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          508 a          712 c          590 g          475 t
ORIGIN      
        1 atacagcggt accgtatcgg cgatggataa ggttcccgtt cagccgaaga tttccggccg
       61 cgtcgtagaa aagtacgtcc aaggcggtca ggatgtcacg gccggtcagg ccttgttccg
      121 catcgacagc cgtacgtacg atgccgcctt ggctcaggca aaggcaaccc aagcccagtc
      181 cgaagccaat ttagccaatc agcagctcaa tctccgccgt taccaggccc tggccagcca
      241 ggatgccgtt ccgcagcaga ccgtaaccga ccaggaagcg gctacgcgcc aacaggcggc
      301 cgtcgtcgcg gctaacgcgg cccaggtcga tgccgctcag gataacgtcg atgataccat
      361 cgtctatgcg cccttcagcg gcaagctgaa cgtcgacgac gtccccatcg gcacctttgc
      421 gacagccggt tcgacggcac tggtcaccat ttcgtcgacc aatccggtct acgtcgaatt
      481 ttccatcagt gaagccgaat acctgcagat gaccaaacag ggggcagacg gcagtaactg
      541 gggcgaccac ctcctgctcc gcctgagcga cggcacgatg tatccttatg aaggccgcat
      601 tgcccaggtc aaccacggca tggacggcaa ctccggttcg attatcgtca aagccgtctt
      661 tgacaacccg gaaaacctgc tcatccccgg cctgtatgct acggtcgttt ccgatacgca
      721 gattcagcgc aatgccttgt ccgtcccgca gcgggctgtt cagcagaccc tcggtaaata
      781 ctacgtttcc gtcatcgacg gcaacggaca ggctcagact cgggaagtca cgccgggcca
      841 gaaagtcggt aaattctgga tcatcaccag cgggcttcag agcggcgata tcgtcatcgt
      901 cgacggctat cagaaagcaa agggagcgac cctcgaccag cacttgctga ccaaggcgga
      961 tatcgacaac gataactcca atacggcttc tgactcgtcg tcaaacaagt agggaggcga
     1021 gaccgtgata tcaaagtttt ttattgacag gcctattttc gccgtcgtca tttccctgat
     1081 catctccatc ctcggtctgc tttccatcgc aaccctgccg gtcgcgaagt acccgaacgt
     1141 tacgccgccc caagtccgcg tgtcggcatc ttaccccggg gcaaacgccg aagtcatcgg
     1201 cacgaccgtc gccagcgtca tcgaacgcca aatggtcggt gtcgacgacc tggtcagctt
     1261 ggaaagttcc agtaatgaca acgggaccta ttcgctgacg gcccagttcg aaacgggcag
     1321 tgacggcgac atggatacgg tcaataccca gaaccgagtc agccaggttt cttcgaccct
     1381 gccgtcagaa gttacggcga cgggcgttac ggtccagaag tcgacgagtt ccatggccat
     1441 ggtattcgcc ctcgtttcgc cgaatgggtc gtacgacgct acgttcatga agaactatgc
     1501 gacccagtac ttcatggatg ccttgaaatc cgttcccggc gtcggcagtg tccaggaatt
     1561 cggttcggac tatgccatgc gtatttggct caatccgatg aagatgagta tcttgaacgt
     1621 cacgccgaca gacgtcatct cggccatttc ggcccagaac acccaggctg ccgtcggtac
     1681 catcggcgct cagccgtcgg ccaacaacca gacctatcag tacaccatgc gtaccgacgg
     1741 tcgtctgcaa acggccgaac agtttaagaa cgtcatcatc cgcaccaact ccgatgggac
     1801 tatggtccgc gtcggcgacg tagcggaagt caacttaggt tcgaaagatt acagcgtcac
     1861 gtccaaactc aatgacagta atgctgccgg cttcatggtc agcctgacgt ctgacgccaa
     1921 cgccatgcag tctgtcagcg gtgcgaaagc cgtccttgaa aaggccaagg aatccttccc
     1981 gtccgatatg gactatgaag tcgtatacga cagcacgaag ttcgtccggg cctccatcga
     2041 agaagttatc caaaccttca tcgaagccct gctcttggtt gccctcatcg tatacctctt
     2101 cctccagagc ggccggtcga cactcattcc gttgatcgcc gtcccggtat cccttctcgg
     2161 gacctttgcc ctcttttccg tccttgactt ttccatcaac accttgacct tgttcgccat
     2221 ggtcttggct atcggcctcc tcgtcgacga tgccatcgtc gtcatcgaag ccgttgaata
     2281 tgaaa
//
LOCUS       DAWDXZ010000297         2279 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9004_length_2279_cov_8.279676, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000297 DAWDXZ010000000
VERSION     DAWDXZ010000297.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9004_length_2279_cov_8.279676"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          568 a          522 c          648 g          541 t
ORIGIN      
        1 gtgacggatt tctttgattt tagaatctgg ccggtgttta atgtagccga catcggcatc
       61 gtcgtcggtg tggctttatt gttatggtat agttggacgc atctgtcgga taaatgaggt
      121 tgaaaaatgg aacataaaga atttgttatt acagcagaag agaagggaaa acgcctcgat
      181 gtctatttgg cggcagccct cgatgtttcg cgaagctatg cgcagcagct catccaaggt
      241 gaagacgttg ttgtaaatga taagcaggca aagtctaatt atcgtttgag tgaaggcgac
      301 gtcgtctgcg ctcagttaga cgtacaggaa gaatataatg tggagcctga gaatatcccc
      361 ctcgatgtcg tttatgagga taaggacatc atcgtcgtca ataaggcacg aggcatggtc
      421 gttcatccgg cagccggaaa ttttgacggg acattggtca atgccctgct ctaccactgt
      481 cagggggaac tgtccggcat caatggcgtc atccggccgg gcatcgttca tcgtttggac
      541 aaggatacgt caggagtcat ggtcgcggct aagactgacg aggctcataa aggtcttgct
      601 gtgcagatca aggcccattc ggcgcaccga acctactggg ccttggtcca tggcaatatt
      661 tccgaagaaa agggcgttat cgatgctcct atcggcagac atccgaagga ccgcatacgg
      721 atggcggtga cctttaaggg cggccgcgag gctgttactc atttccgggt cctcaaacgt
      781 tacggtgact atacctgggt ggaatgtaaa ctggagacgg ggcggactca tcaaattcgc
      841 gttcatatgg cctatatcca tcatcccgtc gtaaatgacc ccttatatgg gtataagaaa
      901 gatgattttc ccattgacgg gcaggcactt cattcccatt cgctcgactt agtacatcct
      961 gtcacgaaac agttgatgca ctttgaagcc ccggcgccgg acgattttct cgaatgcctg
     1021 aagcgggccg aacagaagga gtgacagtat gtggaaagaa aagacactgc tcatggatga
     1081 aaaagcgatg gggcgggcca ttcgccgtat tgctcatgaa atcattgaac ataataaagg
     1141 tctcgacagt acgatcctgg tcggcatcca tacccgagga gttcctttag ccaagcgact
     1201 ggggcaggaa ctgacggcca tcgaagggcg aacggtcccg gtctacgatc tggatatttc
     1261 ggcctaccgt gacgatggcc gcaagagtcc cattgctatt accgatgcca gtaagttcga
     1321 tgctgacggc aagaccgtcg ttctcgtcga tgacgtcttg tttaccggtc ggacagtccg
     1381 gtcggcctta aacgccatca tggaattggg gcgtccgcag ttcatcgagc ttgccgtcct
     1441 cgtcgacaga gggcatcgag aactcccaat ccgggccgat tacgtcggca aaaatgtacc
     1501 gacgtcacgt agagaagacg tggccgttca attgaaggaa acggaccgac aggatcgcgt
     1561 tattctgcgg gaattgacga aataagggac cagaggctgc cgctttgcgg cagtcttttt
     1621 ttattgattt cccctttatt tatgctatac ttaagaaaaa aacatacgtt ttgctatata
     1681 ttcagaaaga aggggttgtg atgaaaaaga ttttaaatga tgtcgatagt gtcgaagccg
     1741 aaatgattca gggtttggtc aaatcggcac cgaagatgct gcgcaaactc gattacggta
     1801 cgattgtcgt gagagccaat aaaaaagaag gcaaggttgc cttggtcagc ggcggcggaa
     1861 gcggtcatga accggcccat gccggttatg tcggagcggg gatgctcgat ggtgccgttg
     1921 ccggtacggt atttacgtcg cctacaccgg accaggtact ggaaggtatt aaagcggtgg
     1981 ccacggagca aggcgtcctc tgtatcgtta aaaactatac cggtgatatc atgaattttg
     2041 aaatggccgt tgaaatggcc gccgatgaag gcatcaaggc agactatgtc gttgtcaatg
     2101 atgacgtcgc cgttcgtgac agtctgtata ccacgggtcg ccgcggtgta gccggtaccg
     2161 ttttcgtcca taagcttgcc ggcgccaagg cggaacaggg ggcatcgctg gcagacgtaa
     2221 aaggcgttgc cgaaaaggtc gttgccaacg tccgctccat gggcatggct atcgagccg
//
LOCUS       DAWDXZ010000298         2274 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9018_length_2274_cov_10.976115, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000298 DAWDXZ010000000
VERSION     DAWDXZ010000298.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9018_length_2274_cov_10.976115"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1578..1587
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          615 a          501 c          648 g          500 t
ORIGIN      
        1 acgccgcggg attcgacgcg gcgggccatc gtattgtaag taaaattgtc tttttcgacc
       61 cgcagaaatc caaaaacgcc gacaaagtag cggcgctctt ggaagatgtc aaggcaaccg
      121 tggaaggcgt tgccgtggcc gagatcatca gcgccgagga ttacgtggaa tatcttgccg
      181 gcaagattcg cggcgagaac ggtaagcccg tcgaatacat cccgcccgag ccgacagatg
      241 aggaaaaggc agccagcgcg gccgcatcca tcgccgctaa atataaccct caactaagcg
      301 ccttgaaaga taacttagtt acggcggtat tgaccggaga cgaggagctc caggcggaaa
      361 ttaaggaaga atacgcggaa ctgatggcgg aatacaataa tgaattggag gcgttgcaga
      421 atggctagga gatgtaactt gtgtgcgcgt aaattgaacg cagaaggtgt ctgcgtcaac
      481 gaaaaatgcc cggcttgcaa aaaggcgaaa atcgataaag cagccgaaga aagcaaagag
      541 gagactaaat gagcgatgtc ttacttgagt ttttccggga catgctcccg gcagccgaag
      601 aggtagaatg gggggccgcg gtatccatcg ccggcacctt aatatctgga ttagtagggt
      661 gggatgccgc tatggaagcg ttgctggtcg ctatggccat tgattacatt acgggcgtct
      721 tagcggcgta tatcaatcct gatttggccc ttaacagcca gaaggggttc cggggtatct
      781 gcaaaaagat aactatcctg ttactggtag cattggccca tgagattgat aaggcaacag
      841 gacagcccgc catccagtcg ttggtggtgt ggtttttcct gggtaacgag gggctgtcaa
      901 tcctcgaaaa tgccagcaag gcaggcctgc ccatccccaa aaaattacac gatacgctag
      961 aacaactagc ggatagcaag gaggctaaaa aatgaaggta tttcttaacc ccggccatga
     1021 ccggaaatat gattccggcg ccgtacatct cgatgacagt ggtaacgtag acttaagaga
     1081 gtgcgacgtc gccattgaga tcggtagcgc tgtccagcgc tacttagagg ctgccggctg
     1141 caaggtaatg ctcatgcaga gcgacaatct ggcaccgacg ccgcccggtc gtagcgagta
     1201 cgccgatcgg cagggcctca ctgtcactga aacagctaac gagtggggcg ctgatgtatt
     1261 tttgagtatc cattgcaacg cctttaacgg ggccgcccgg ggcacggaga cggaatgtta
     1321 cagcaggatg agtaacggcg gcgcacttgc ccagtgcatc caggtacaga ttatcgacag
     1381 catcgacact accgatcgtg gagtccggga gcgtccggac ttgtttgttc tgcggtatac
     1441 ggcaatgccg gctgtcttgg ttgaggttgc atttatcgac aatgacgacg atatgcgcct
     1501 cttagttaac gattgggata cgattgcccg ggccattgcc cgcggcgtca ctgattatca
     1561 gtgtatgcag tagtggannn nnnnnnntgg aggtgtaacc atgagtcagt ggactaagtt
     1621 tagggatgga ttgttagcgg ccttagaggt ccaagaagta gccgacacgg ccaaaaatga
     1681 gctggtcaaa aatctcgccg gcgaaggtat gacagccctg ggggcagtgg ctgataagtt
     1741 ctccgctcag ctccaggcac aggccaaaag tgaggccggt tggaataaga ttagagatca
     1801 gtttgtcctt ccgctcttga tctccggcgt cctttggggc gccaagctcg tcattactaa
     1861 gagctcgacg cctaagcaat aacatatata gtattatagg agccctgcgg gtcggtgctt
     1921 tgcgccgatg ctgcggggct tctttttttt tacaattttt taaaaataca ccgatatcgg
     1981 tattgacagt acaccgatat cggtgtataa tgtaaccaag gaaaggggat aaagagaaac
     2041 cccagataac agttaaatct aaggaggaaa ttaaaatgac aagacattac accgaagaag
     2101 aaattagaag agctagatta gtagcggaca ttaataactt tcccgaattg atgggggctg
     2161 aagaagatat cgattgtgtt atggtaatta gggtcagggt atggagagaa ttagataaag
     2221 attatccaaa acttaagctg tatggcccgt cgcatggtga attctatgag tggc
//
LOCUS       DAWDXZ010000299         2264 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9051_length_2264_cov_7.767768, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000299 DAWDXZ010000000
VERSION     DAWDXZ010000299.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9051_length_2264_cov_7.767768"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2022..2031
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          517 a          601 c          610 g          526 t
ORIGIN      
        1 tcacgtggtt attccttcca ttataaccgt cacggattaa aggggcgtaa aaaaaagacc
       61 ctcgcgggtc ttttttcatg aaagaatcat tagattttat gaatggctaa aaaggcgacc
      121 aggactatga ggagtacgat ggcaatgata cgggtaatcg aggccaagga aatccgaaca
      181 tccttctgcg cccttctttg cggccgacgt tcactcatac gccgggccat gccatttcct
      241 tgccgcctaa gatatgggcg tgcatatgat gaacggtctg accggctttt tctcccgtat
      301 tgaatacgac gcggaagccg tccttatcga gattcatgac ctgagcgact ttttggattg
      361 catagagcat cttgccggct atatcggcat catccggcgt cagggccgca atgctctgga
      421 cgtgtttttt gggaatgacc aaaacgtgta ccggtgcgac gggattgatg tccttaaagg
      481 cgaagaaatt gtcgtcttcc caaaccttag tgctggggat atccccggcg atgatcttgc
      541 agaaaatgca gtcttccata ataaattcct ccttctggat acggcaaatc aggcttacga
      601 gccttccctc aaagggcctc ggccaagagc ttcttgccat ccgttccgac aagggctacg
      661 gaaacgaccg tccgctgtga gccttccggc agtcccttca cggcgacgcg ttcatagttt
      721 cccgtaaagc cttccccttc agtcccttcc tgagtttcga tgagaaccga cgccttggtc
      781 ccgacaagag attcgaggag cgccgctttt tgacgggccg acaattcatt gaccaattcg
      841 acgcggcgtt tcttttcagc cgtatcgatt tgaccgctca tggttgctgc cggcgtccct
      901 ttacgcggcg aataaggaaa ggcgtggata tgggaaaaat cgaggcgttt caaggtctcc
      961 atcgtctctt caaaaagggc ctcggtctca ccgggaaagc ctaagatcag atccgttgaa
     1021 acggtcaggc cttcgatgcg gctgcgcaga cgagcaatca aggcttcata ttcggcaacg
     1081 gtataatggc ggttcatagc cttcaacacg gcatcggacc ccgcctgaag cggcaggtgc
     1141 agatgacggc aaatccggtc cgagctgttc atgacggcga tcatctcgtc gtcgatttcc
     1201 agcgattcga tagagcccat gcgaatgcgg gatacgtccg tttctttgac caaacggtct
     1261 aagatatgtg ccaaggacgg gtgatcgtcg agatcgcggc cataggcacc gaggtggatg
     1321 ccggtcagga cgatttcctt aaagccggct tcgacgaggc gtttcgcttc ggcaatgacg
     1381 gcatcgggac ggcgggactt caaagcacct cgcgtatagg gaatgatgca gtaagcacag
     1441 aaattattgc atccttcttg tatcttcagg tctgcccgcg tatgctcgac ggccgacgga
     1501 tagaggggaa tttcctcaaa ctcatcgtgt tggcgcacgt catggacagc cgtgaaagct
     1561 tcatcatggc cattagtgag taaatcaccg acgatagaga cgatgcgttt tcgttcgttc
     1621 gtcccgacga cggcatcgac ttcgggcatg gacgaaagca actccgggtc aagctgggcg
     1681 tagcagccgg tgacgatgac cttagccgct tcgttgcgct tcttggtccg ccgaatgagt
     1741 tgacgtgact tcttttcacc gacttgggtg accgagcagg tattaatgat ataaacatcg
     1801 gcagggccgt taaaatcagc ttcttcaaag ccagactgaa gaaataagcc ccgtatgctg
     1861 tccgtatcgt attggttgac acgacagccc agggtcgcaa aagcaatagt tggcattact
     1921 tgaatcctct ttcgtacata atgatagata aggccgccaa cgacgccgtt tcagcccgca
     1981 ggatccgcgg tccgagagag accgtgcggc accaggcaaa gnnnnnnnnn ntgttcagcg
     2041 taggcgattt ccttttcggc ataaccgcct tcaggaccga tggcgatgac gacatcaccg
     2101 gtccgcacgt cgcggcaggc ttcggccagc ggcgccgttt cccgttcata gggaaggatg
     2161 cagcgcgtat cggcataagc gtcgacaaag tccgtaaaat tcatgaccgg tgccacttca
     2221 ggcacgtcgt cgcggccgca ttgcttagcc ccttccaggg ccag
//
LOCUS       DAWDXZ010000300         2263 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9054_length_2263_cov_6.678895, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000300 DAWDXZ010000000
VERSION     DAWDXZ010000300.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9054_length_2263_cov_6.678895"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1535..1544
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          515 a          573 c          592 g          573 t
ORIGIN      
        1 agtagttcat agacagagga tacgacatcg tcccaagttc cgatttgggt atgctcaccg
       61 gcaatcgtat cgcggccggc cttgcattga cgcgcaacgg acgttggcga cgtgccgtta
      121 tatgtgttac gctgtttgac gcacgtttcg atatccaagt aatgatggat atcttcggcg
      181 aagagagggc tcatggcctg cagctctgac agcgataact gctgtaatac cttgccctgt
      241 tcgatgcagt agtggacggc tttcccgacg acggcatggg cttcacggaa aggaagtcct
      301 tttttggcca ggtagtcggc catatcggta gcgttcgaga aatcgctttc cagcacttcc
      361 cgcatatgct cgccgttgac ggtcattttg tcaatcatgg cggcatagat ggtcagggca
      421 aatttcaaat tgtcgatggc atcaaagacg ccttccttat cttcctgcat gtccttgtca
      481 taggccatgg gaatcccctt catcatagtg aggacgccca tgaggtggcc gtaaaagcgg
      541 ccggtcttgc cgcggacgag ttcgcagatg tcgggattct tcttctgggg catgatggac
      601 gaacccgtgc agtgagaatc gtctagttca atgaatttaa attccgagct cgaccagagg
      661 atgaattctt ccgacaagcg gctgaggtgc atcatgagaa cagcggcaaa ttccaagaaa
      721 gcaatgatat aatcccggtc gctgacggca tcgagactgt tgtcatagag cttcccaaac
      781 ttgagcgtct cagcgacaaa gggctgatca aggggatagg tcgtcccggc cagggcaccg
      841 gcgccaaggg gcatcatatc ggccatctcc caggcatatt tcaagtggat gaagtcacgg
      901 gacaacatcg agaaataggc catgagatgg tgactgaaga ggatcggctg agcccgctgc
      961 aggtgcgtat atcccggcat gatgacgtcg ctgtactttt cagcagcctt caccagggac
     1021 tcttggagct tgaccaagag gtcggcaatg gcagcgattt cccggcgaat atagaggtgc
     1081 atatcgaggg ctacctgatc gttgcggctc cgccccgtat gaaggcgttt tcccgcttcg
     1141 ccaatggcat cggtaagtct cttttcaata ttcatatgaa tatcttcgag ttcgacagaa
     1201 aaatcaaaat tcccgtcctc gatttgctgg aaaataacct tcaagccttt tacgatgacg
     1261 tccttatccg cttccgaaat gatgccttct ttggccagca tcgtcgcatg ggcgatgctg
     1321 ccgcaaatat cttcggcgta catccggcag tcgaactgaa tggatgcatt gaaggcttct
     1381 acacttttat ctgtggcctt agtgaaccgg ccgccccata acttcatatc attcgctcct
     1441 tattatttac cctgcttttc aacatgctgc ttcatcaggg cccgcatctt gaggggcagt
     1501 ccgaagaggt tgatgaaccc ttcggcatcg gtctnnnnnn nnnngatgat attgcccttg
     1561 tagagtttca aaataacctg accggtaacg gtctgctgcg tttcatcgac gaaggccgaa
     1621 agagcttcgc gaagcggcga gaaccattta ccgtcataga cgagttcggc aaacttgttg
     1681 gctgccgttt ctttatagtg gaaggtgtcg cggtcgaggc agaggtattc caattctctg
     1741 tgagcataca tgaggatggc accgcccggg ttttcgtaaa taccgcggct cttcatgccg
     1801 accaaacggt tttcaacgat atcgacgatg ccgacaccat tgcgggcacc gatttcattg
     1861 aggaccgtaa tcaaggtaac ggcgtcgtgt ttttcaccat tgacggcgac gggaatccct
     1921 ttttcaaaat cgatggtaac gtattccgga gcgtccggag cctgttccgg cgtcttggtg
     1981 accatgtaga ccatgtcttt cggggcattg gccggatctt cgaggtcatc accttcatgg
     2041 ctcaaatgcc aaatattcca gtccatgctg tagggatagg gattttcttc cgatttatag
     2101 tcgataggaa cgttgtgggc ttccgcataa gccagttcgt cttcacgaga tttgaggtcc
     2161 cacatccgcc acggtgcaat caaggtcatt tccggagcca gggctttgac cgtcaattca
     2221 aagcgaacct gatcattccc cttacccgta gcaccatggg caa
//
LOCUS       DAWDXZ010000301         2239 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9128_length_2239_cov_8.657509, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000301 DAWDXZ010000000
VERSION     DAWDXZ010000301.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9128_length_2239_cov_8.657509"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    580..589
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          593 a          576 c          459 g          601 t
ORIGIN      
        1 gtccatcaaa atgcgactga acgtcggaaa aaagagggac tccccctcgt cgtcagtatt
       61 gacgaacccc ctgaatcgac gatataatga aattacaaat tgggctgtga atcccaaaaa
      121 attgtttctc ctgccttgtc ttcgtatgaa ggcaaaaaag aaggcactct ctattgaggg
      181 tgccttttgc atgtccaaat aagccaaccc caggggcaaa ggaattctaa gtgatagcac
      241 ctactcccat tcgggaaccc agccttatcc ttcacaagtt gacttctgta accattatac
      301 caaataacgc agtcaatgaa aatcagcagt tcttcgttaa gagcaaaaat aacctttttt
      361 ctgctcttaa ccagccttaa gagcagaaac gtcttacttc ttgtccttag ctattttcaa
      421 attcgcctcc gtgggatgaa gattactacc acctttagcg gtatctttct caatgctctc
      481 catatttctg gctcgttgtt tccgatattc attcaacccc cggatattct ccggatcacg
      541 gtagaattca agaatatctt tgataaataa atccaacatn nnnnnnnnnt cttctaacta
      601 actctctcat agataataag tttttacagc actaaacccc tcgaattcga ggggtttaga
      661 aataagagat ataaatgtat ttaccttctc cccaaaagat gaattgatgg ctcgtaagat
      721 cccattagcc caaaatatta aaaaatgata cccttccata tttcaggcgg tatttgctca
      781 atcggtttat tccggcgaat ggcacgacgc aattcctttg ccccttctat aaaattcaga
      841 cacgaaggac gcaaaggctc gtaatgcatc gtgcgctcca aggagtgctc cccgaatttc
      901 gccaagtact ctttttggac ctcaaagtac tcttcaatcc ccggatggac gtcttcaaaa
      961 cgtttcggtt ccatcatgtt tcgcctcctc attcaaaaag tctccgttca tgttacgact
     1021 ttatcagctc ataccttgtc gtgcgtccgc cgccaagctg ggcgatttgg cccctatcgg
     1081 ccagcgtttt cagaatgcgg cctgccgtag actggctgac gtccaacaat tcctgtacct
     1141 ctttccgggt aatgcgcttc ttttcttcca gaagctgcat gattttatcc tcgtcggaag
     1201 tccggaacgg ggctgccatt tcttcgacaa tcatcggtat ctcaacggct ttattcctga
     1261 aagtcacatt gggaagaatg attttgaagg catttgggct agtcttaatt tcgggcttca
     1321 ccggtttatc ggcataggca tccatcattt ttttcacacc tattccgtca gcttcaatga
     1381 gcttcaagtg gtaaaagacg ttgtgcagat gcgggttgcg gcacagggac acgcccatgt
     1441 tcaaatcttc cgggacgaag ccgggaagga gaccgccaac agacaccatc tcaacgcggt
     1501 catcgtatat gtggatcaaa gtatcggcac caaaagaata gtttcggtgt agcagcacgt
     1561 tcatcaaggc ttcccgaagc gctatgtccg gataacctcg gcgaccatcc gtataacgga
     1621 agtgatgtcc gctcgtgtag ttctgattgt gaaaattgac ataagaacag gcggccttca
     1681 tttgcttcag gagagagccg gaaaaatcct gacgggcctt aaacacagtt ggactcgtcc
     1741 catcaaagac tgccgccctt atcgtacagg gattctggtc agaaaggaga aaacccagat
     1801 tcgtatagag accgtcaccg gatatgaggt tcaaggtctt catctgctgt tgatcaaagg
     1861 tcatattcgc gtcagcaaat tcacctttca aggcatcaaa agttaagaac tgatcgatgg
     1921 atcgtctctc ttcaaaactg tcgccatcga ttgcttcaat catccggcga atggccgtct
     1981 tcgttgccgg aacggtctca tattcctgac gcacataaac gccctccggc tgcagtccct
     2041 tatttgccaa aaaatatgga caattcgtgc ctcgctgaac gcggaccgca acgacggcgt
     2101 ttccgtcaca gtttaacgtt tcataatgaa tgaacatcgt gacgtcaggt tttatggcgt
     2161 cccgcaccat attcgaaatc tgcaaaacac aggcggcggc atcttctacg cctaaaacgg
     2221 ccccgtcatc cgccacacc
//
LOCUS       DAWDXZ010000302         2223 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9178_length_2223_cov_6.261993, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000302 DAWDXZ010000000
VERSION     DAWDXZ010000302.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9178_length_2223_cov_6.261993"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          522 a          625 c          558 g          518 t
ORIGIN      
        1 tcgccttatc ccggcaattt gccgaaaata acgtcatcga attctacccc gtcgaagacg
       61 ccaaccaccc cttccaaaat cccaatcata tggccctggc catccataag atcatcgaat
      121 tcttccaacc ggaatcgtaa taatccatca aaaaaacgac ctgttgacga aagcatcgtt
      181 ctttcatcaa caggtcgttt cgtatatcat cgtcttttaa aaggaacctc cgcggccgcc
      241 atggccgcct ccgcctcctc ccagattgaa gcttcccggc ccgctgtcat cgtcatcacg
      301 acggcgaggt ttgggaatgc gctgtaccgt gaggaataag aaggtatcgg tctgctcaga
      361 caactggaag ctgtgcgaat caaaataggt atcggcagcc gttgcccttc ggacgttgct
      421 catcctcatg atgaggtagc tgcggaagat catggcagcc agcgcggcca agacagccgc
      481 cgtcgccaaa tccagccatt gataagcatc gtccttatca taggccttgc cctgatcgtt
      541 atagtaggcc aagagctggc ccgacgaatg ggcatattcc tcgaaggcct ccatgtaatt
      601 tcccttcttt aagaggggca ggacggcatc ttcgatatgc ttcaacccat ccgtatcgct
      661 gaccttgtcg cgcatggtac ggtccgtcac gatataccac tggcgctggt ccatggctac
      721 cacgaagacc atattgccat tttttccatc gccgtactgc tcatcgagga catggtcggc
      781 atagtcaccg attttcatgc cgtgcgtcga acgaacgatg taaacggcca ggcgcacatc
      841 gtaggtaaag ttgacgttct ttaaggacct ctccagttgg ctttgctgcg acttcgtaag
      901 gacgccggcc tgatcgacga cattgggaac gccttccgcc agggccgtcc ccatgaggat
      961 caaaaaggat aggaccaaca gggccgtccg catgatagtt ttcaacacaa gactcccccc
     1021 cttatattgg ccacggttta ccacccataa tggcgtgaac aatcagatag aataccggca
     1081 gcgtaaccag catggcgatc agaaaagccc agtaggattt caccttatca tagggcagag
     1141 aaccgacgaa gcggcccgtc tggccgttca tcataaaggt atagggcttg tcctgatagc
     1201 gcgtcgtcaa gatccatacc ggtgccatag cgtattccac cgagtcttca tctttatgga
     1261 cgaagccccc ttcgaaggag cggcgctgat gattgacgac ggtattgctg aggaggtcaa
     1321 aggaactttt ctctaagcgg gaatcggccc gaggcacggc ggcttcggca tcgacgtcgt
     1381 acttatcggc taagcagccg gcaaagtagg ccgagttaaa gggcacgatt tcgctgtagt
     1441 caaagggctc gatactctcc atatagaggt cattgatttt cttactgccg tcgacgggaa
     1501 tccggttgaa agtcagctga ccgatgcggt cgcactgata gacccttgtc tcgacgatgc
     1561 gttcatgttc cgtctcaaga tacgaatcgt cttcagcccg aaaggaggcc gacgccgtaa
     1621 ctttggcatt gaacagccaa aaagggacgt acatgggctg aacgtcttcg atgcggttgt
     1681 tcaccgtaaa ggccttggga aggagccaat ggcctctata gaatcgtttc aaggccgcca
     1741 cggcctcgtc tttcgtcttt ttaaaaggaa tgatgaagtc gggccgcagc atccccgaaa
     1801 aacgactggg aatgagggtc ggattgccgc aataacagca ttcggtagcc atcgtattgt
     1861 catcgcagac gatttcagcc ccgcaagaag agcaagtata gcccttcagc cgggccgctt
     1921 cctcatcgct ccattcgttg ccggcctttt cggtctgcca cttagcctgt cgggccgcat
     1981 cggcttcagc cgccaattgt tcctttttcg cgtacagccc ttcgagtatg gcgacatcga
     2041 atttcgtacc gcagtagtca caggtgacag tctgcgcctc gggcttaaat tgaatcggcg
     2101 ccccacagtg agggcattta taactgactg acgtatcggc catgggaatc acctcaatct
     2161 tcaggtttct tcgtaccgca gttcatgcag gatttccccg tattcccttg ttggccgcag
     2221 gac
//
LOCUS       DAWDXZ010000303         2222 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9182_length_2222_cov_7.745270, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000303 DAWDXZ010000000
VERSION     DAWDXZ010000303.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9182_length_2222_cov_7.745270"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          604 a          537 c          506 g          575 t
ORIGIN      
        1 atgtattttt cttaaaatag ataatattat atagtataat atgtgttggt taaaggcgaa
       61 tatttgtata atcttcattt tagtataaca tattaattgt cctaattctg tcataattaa
      121 tatttcttta caatattatt ataatatatt attaacataa gtgtaattca ttcatgcctg
      181 aaagttatac agaagattcc ctaggactta ggcataagaa tctgccttgg cttctcatac
      241 tcctctgcaa aaaaagccct gctccaccgt catactgatg acgatgaaac agggctgtca
      301 ggcaatacga tgtatcgtcc gtattgcatt gatagtcgtc tactttttat tcggccgtta
      361 tcttcgatac catagcggcc ttaaattcat ggacttcata gcgagccata tcgtccttag
      421 caaacacgtc ttcggcaagg atggcctcga gttcctctcg tgaagacaag gcagacagca
      481 tgacgccggc cttaggaata tctaagtaag gaccgacgag gacaaaatgt ccaaggttaa
      541 aatgtttggt gaaccattcc gcatgctgct gaaataaaga ttcagcctgg tcctgaggaa
      601 tgttttccgg attcaaatat tcgctgatga taaacatggt gcatgacctc cttttcagtc
      661 actgagaccc tttgatactt atagcttacg gcatagagtt tagtccaggc aaggcgttgc
      721 ttagaaaatt ttgacatctt tcgtcagttt cggagagaag gatttttatg tcgcatgtcg
      781 aatttgtaac atatggattc tttttcagac ctgataaaca gattgccctt ggcaatacag
      841 acgatttccc atagggattt tctaaaggag gcgttttaat gaagtggaag aaagttcttt
      901 gtaccggcct gtttacgggc atcctcacgg tgacggccat gagtggcctt gcccttgatt
      961 tgaccaagga aaccgacgtg atcagccgca acccgtccaa tgtctatacg atgtacctcg
     1021 gcatgtcgga cagtgaattt aagcagaatt tttccggcgt cagcggctgg aattataaca
     1081 actcgacaca gaccaagatg tggaagctgc cgagtatttt agaagcctac gacaatggcc
     1141 aaatcggcat cgtccatacg tacggccgtt ctgaaaagca cgaaacggtc ccttacgtca
     1201 tgaaccgcga agctttgatg gccgacttta aaaagagccg ccccctaggg aattattccg
     1261 tcgtgctgaa gaggaccgtg aaatccgatg acgtcaaaaa attgaaatcc gaggatacgg
     1321 cagcgattct gaaggaaggt ctggatgatt ttaaattggc cgccgacaac atgaccaaag
     1381 ccttaggcaa ggggccgacg gggtcgcaat atttggacgg tcagatcaac ggcaatgaat
     1441 ttgccaagac catcatctac tcctggtcga caggcgatcg ttcgggctat agcctctcta
     1501 tttcgtacaa actcccccag acgattgacc gcagccgccc gcgggccgtc gattatacag
     1561 agcatcctga gtttgatccg gacgacgtcg tcatcaccga gcttagcatg cagcattatg
     1621 actggaatat agaagataaa taggaggttt ctatgttcga tatcttgaag accatcggcg
     1681 aagtcctcat cgccttgttc attcttcgct atttgattca catatacaat accctcggcc
     1741 gcacgcgcga gcccgtccgc aaggccttgg ccgatatttc aacctacaag gcccaacggg
     1801 aagcgacgct ctatcacctc tatgtcattg ccgagcagtt cagccagcgc gaaagcaata
     1861 ccctgactca ggccagcctg gcgaacatgt ctcggcaaaa cgctaatttc caggccttgg
     1921 ccgccgccta tccggagctg aaatcgaatc agacctacct gtccctcatg gacagcgtcg
     1981 gagcgctgga gctccggatt caagcgagcc gtcaggaata taatgaggct gtcagcaact
     2041 atcagaccct gcgcagtcag attccctact gcctgctggc gcccctcttt ggctttaagt
     2101 cggccgaata caatcaaaaa gaaaaggatc ttgaagccag tccctccgaa atcaagacgg
     2161 ctccggacgc actgcctccg gtcatcgaaa gtccggtcaa agaggttatg ccggaaccgg
     2221 tc
//
LOCUS       DAWDXZ010000304         2215 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9210_length_2215_cov_7.702315, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000304 DAWDXZ010000000
VERSION     DAWDXZ010000304.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9210_length_2215_cov_7.702315"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1468..1477
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          571 a          561 c          545 g          528 t
ORIGIN      
        1 atttgaaatg gaaaactacg ccaaaggtac ggaagccgtt gctaaagcag ttgctgattc
       61 cgatgccgtt tccatcgtcg gcggcggtga ctccgtttcg gcagtcaaca aaaccggtct
      121 ggctgacaaa atcagccaca tctcgacggg cggcggtgct tccctcgaat acctcgaagg
      181 caaacagctc ccgggcgtcg ttgctttaag cgataaataa gtcgtttcat actatttcat
      241 atacatacgc cggatgcccg gaattcccgc tgtcatgacg gactgctccg aagagatcgg
      301 gaccgggcag atctggccaa ggaggaaaat ttattatgcg taaaactatt attgccggta
      361 actggaaaat gaatcattta ggcgctgacg ccaaggctac gattcagggc ctcgttgaaa
      421 aaatgcccaa agacgtaaaa gctgaagtcg tcattgcccc tgttttcccg tacctttcga
      481 cggctgttga aatgactaag ggcacggcca tccatgtcgc tgctcagaac atgcactggg
      541 aagaaaaggg cgccttcacg ggcgaagttt ctccggccat gcttgtcgat atcggcgtaa
      601 cacacgtcat cctcggccat tcggaacgcc ggacctactt caatgaaacc gatgaaacgg
      661 tcaacaagaa gacgaaagca gccctcgctc acaacttgac accgatcgtt tgctgcggcg
      721 aaacgctgga acagcgcgaa gaaggcatca tgcagtcttg gatcgaaggc cagattgaaa
      781 atgccctcaa aggcatttct gaagccgaca tcaagaaagt cgtcatcgct tatgaaccga
      841 tctgggctat cggcacgggt aagacggcca gcgctgctca ggctgaagaa gtctgcgcca
      901 ttatccgcaa aaaattagct gctctctaca gcgtagctgc agctgaagac gtatccgtcc
      961 tctacggcgg cagcgtcaaa ggcggcaacg tcgctgaact caccggttct gccgacattg
     1021 acggcggcct cgttggcggc gccagcctca aagctgatga cttcttagcc atcatcaaca
     1081 atgccgttgt aaaataaatt gaactccatt ttcccccgca cctctcttag cacttgtgcg
     1141 ggggtttttc tgtctgtggc cggacttttg catgaccgcc cctactttgg tacaataaag
     1201 actaggtgat gaaggaggta ttcatttgaa atatatttta tacgtctttt cctttttgtg
     1261 tatggccatc ggcggcgggg cttggttctt ttacggacga gcagtgggag ctgtcggtat
     1321 cgtcctgtcc ctctatattc tctatcgact ggaacggcag ccgcgccaac tggcattgaa
     1381 actgacacga cgcaagggaa agtcagccgg ccaatatggt acatcttttg aaaaagccgt
     1441 agctgatttt aaccgcatgg aaggcgannn nnnnnnntga ccgacccgga ccttcaagcg
     1501 acgaccgcca atatgcagca tattgcccgc aatctcctct actatttgaa acaatatccg
     1561 gaacggattc ccttggccga gccttttatt aactattacc aggaccgggc cgtcttttta
     1621 gtccacaaat ataagcagct caccgtgacc ggtcttaaga cgcagaaggt ggttgcgaca
     1681 aagaaaaagg taagggactt gctccttcag atggatgcgg cctatgaaaa tcaatttacg
     1741 gaagtcctcc agggcgaact gcaggtcatt gatggagaag ttgtggccat gacccgcaat
     1801 ttagaaaatc ggggagtcta tcgcgacccc ggtaccggat cgagtagggg cagacaaaat
     1861 gccttttcac ggtggaaaca acgagcttcc aatcgcttcg atgcctatac ggacggaacc
     1921 ttttcatcca ttacgccgga actgcgccat aagatcaatg aagaacggct gattacggca
     1981 gccctggcct tctttttagg ttccttcggc attcatcatt tttatatgaa gcgaaatacc
     2041 cgaggctatg tgtatgcctt attttgctgg accttcatcc cgggattctt gggaattatc
     2101 gaaggaatgc gctttgcgaa tatgacgacc gatgaatttt attacgcctc gtacctgcac
     2161 aaataccgcg cataattgac aatactttac aaaacatgta attgtgaaaa ctatg
//
LOCUS       DAWDXZ010000305         2215 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9208_length_2215_cov_11.136574, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000305 DAWDXZ010000000
VERSION     DAWDXZ010000305.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9208_length_2215_cov_11.136574"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    310..319
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          542 a          530 c          564 g          569 t
ORIGIN      
        1 aatgatacaa gatagtacga ggtttgaaaa gccgcttata aggtccctac caaatcgatg
       61 cccggtttca aggtcttagc gccgggtgtc caccgagccg ggcacacttc gtctccgtgt
      121 tcacggacga actggcaagc ctgtaccttg cgcagcagtt cttcagcatt tctgcctaca
      181 ttgccggcat tgacttcata agagacaatc tggccttccg gattgatgat gaaggttccc
      241 cgttcagcga cgccggcgtc ttcgatgagg acttcaaaat cacgagccag ggcatgggtc
      301 ggatcggccn nnnnnnnnna tatccttgat gagcggcgag tgatcatgcc aggctttgtg
      361 tacaaattcc gtatcgcagg aaacagaata gatttcgcag cctatttttt tgaactcttc
      421 ataaaagctt tcgaggtcgg ctaattctgt cgggcacaca aaggtgaaat cagcgggata
      481 aaaaaagaat acgctccatt ttcccaagag gtcttccttg gtaacggtga tgaatccgtt
      541 atcatggtag gccttggccg taaaatcact gacttccttg ttgattaatg acataagtat
      601 gaacctcctt ttaaatgaaa atcactatca ttataaataa aagatatcat gaattgcctg
      661 aaaaatcaag gcaaacataa aaactttttg taataactca ggtctttcaa aggacaaaaa
      721 aggacgtgag caactgtact cacgtccatc cgaggcagct gctgtaaaag acatcgccac
      781 tatataagga agtttctatg ggtatattat tttactgtcg tccgtcccgt ttcaggaaaa
      841 agggcaaggc cacttccggg aacaaggtat aactcaggac gtcttccatg ctggcattgg
      901 ggaagccttc gttagccagg gacttcttag ctgcttccaa ttccggagcc aagtcatcgg
      961 ccggacgatg ggtaatgacc ggttcgtcgc caatgcacat cttccggaat gcttcatcga
     1021 tcttgccggg tacacggcca tatttgccgc gaacgatatc gcgcacttca ttggttacga
     1081 ttttataacg gccgtagagg acgtttaagg cagctgctga accggtgatc tgactggtcg
     1141 gcgtaaccag aggcggataa ccgaggtcac ggcggacatt cggcatttca gccaggactt
     1201 cgtcgaagcg gtcttcggct ccttgggctt taagctggtt atagaggttg gtcagcatgc
     1261 cgccgggaat ttgatatttg cggacagccg ggttgatctg actgggcatg gtcagtttaa
     1321 attcatcggc cagttccttg cggacattct taaagtgatt gacaataggc cacaggcggt
     1381 cgacgtcgat gcccgtgtcg taaatactgc catttaaggc catgaccatc gattccgtcg
     1441 ccggctggct ggtgatttca ccaaagggtg acaaagcggt atcgatgatg tcgacgccgg
     1501 cttcaatggc cttcatgtag gtcatttcac caagaccgca ggtacagtgc gtgtgcagtt
     1561 caatgggaac ggtcagatgc cgcttcagca tgcggatgag gttatacgcg tcataaggcg
     1621 acaggagacc cgacatgtcc ttgatacaga cggaatcgac gcccatatcc tgcagttcct
     1681 gaccgacttt ggtgaaggat tccatggtgt gaatcgggct gatggtgtag accagagccc
     1741 cctggacgtg gactgatttc gtgtctttcg ccgctttgat ggccgcttcc atgttgcgcg
     1801 gatcgtttaa ggcatcaaaa acgcggatga tgtcgatgcc gttgtcgacg gcacggttga
     1861 caaattcata gacgacgtcg tcggcatagt gccgatagcc caagatattt tgaccgcgaa
     1921 gcagcatctg gagaggcgtc tttttgacgt gctgcttaat cgtccgcagg cgttcccacg
     1981 ggtcttcatc gaggaagcgc atacacgtat caaaagtagc tccgccccaa cattcgagag
     2041 cattgtagcc taattcatct atcgcttcca gtacaggaag catctggcga aggcgcatgc
     2101 gagtcgctgc aatggactga tggccgtcgc gcagaaccgt ttccgttatt tttactcttg
     2161 ccattctctt tcctccatag tatatctatt gcaatgtgtt tattttaaaa tcata
//
LOCUS       DAWDXZ010000306         2207 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9246_length_2207_cov_9.547862, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000306 DAWDXZ010000000
VERSION     DAWDXZ010000306.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9246_length_2207_cov_9.547862"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          498 a          521 c          583 g          605 t
ORIGIN      
        1 ttcgacgggc atttcactga tgttgaccgg aaggtcgatg aaggtggcac ccggtttttc
       61 acgttcggca tatttaaagg ccaagcgggt gatttcggcc atcgtatcgg ggcggacgac
      121 ggtataggac cggcgggtga tgggttcgaa catggcccga agatcgaggt actgatgagc
      181 cgtgatgtgc atcttgtccg ttccgacctg gccggagatg gcgacgacag gggctccgtc
      241 gcagtcggca tcggcgacgc cggtgatgag attggtagct ccgggcccga gggttgccat
      301 gcagacgccg gccttgccgg tcagtctgcc gtagacatcg gccatgaagg ccgcgccctg
      361 ttcatgacgg acggtgatga attcaatctt tgaatgctcc aaggcattga tgatggctaa
      421 gttttcttca ccgggaatac cgaaaatata atggacgcct tcttcttcca ggcattcgat
      481 gagaaactct gccgtattgc gcatcttttt ttgtaccatt ccgagaacac tcctttaaag
      541 gtaatggata aattaaaatt gctgttgtaa cctgtattat aatatgggac agaacctttt
      601 tcaatgactt ccttgtatta tcaggatgta caattcaagg cagctggaat tggtcgtatc
      661 gaaacagcca ttcaaaaggc tcgtcatgaa tcctttatga cggttgtttt cccgggattc
      721 gattggaaag gataggagct tagatgaata tattgatcag cagttgtctc ttaggacagt
      781 attgccgcta tgatggaaag accaagacct acgatgtgat aaggccttta ttgggccgtg
      841 acgaccttca tttcattccc atctgtccgg aacaggcagg tggccttgcg acgccgagac
      901 ctcctgctga acgacgcgga gaacgggtgg taacggaagc cggtctcgat gtgaccgagg
      961 cctatgaacg aggggcagcg gcggctctct atctggcccg cctctttcac tgcacgaagg
     1021 caattttaaa ggccaagagc ccgtcctgcg gctgcggcct catttacgac gggacctttt
     1081 cccggaccct tactgagggc gacggcgtga cggccgccct cttaaaagcc gaaggcattg
     1141 aggtttttag cgaaggggac cctttagaat acttgctttt ataacgtcgg tttgctacaa
     1201 tatacgagta cgcaagtgag gaggtttcag ggtgaatacg tttttgcatg aagtttgtga
     1261 atggctttat tctattgtca tcgccttggc cattgctatg gtcatccata ttttcttctt
     1321 ccagccgacc cgcgtttccg gcgagtctat gatgccgacc cttaaaaatg gggagtacct
     1381 cattgtttct aagtggagtc acgtcatggg agaggtccct aattatggtg atatcgtcat
     1441 cattgacagc cgcgtccagt atccgcgcac ttggaaagat gatgtcgctg aaccgatgaa
     1501 caactacttg gcgttctttg accacgattt acagacgaaa aatgtttggg tcaaacgagt
     1561 tatcggccga cccggtgata cgctggcttt ccacgacgga aaggtttggc gcaatgggga
     1621 acctttggat gaaccctata tcaacggtcc tatggaatac agcagtcggg aagaagttaa
     1681 agtcccggaa ggctacgtat tctgcatggg tgataaccga aaccacagca gtgacagccg
     1741 ttttatcggt cctgttccta tcgatcacgt gttgggccgc gttatttggt aaccggtctt
     1801 tacgacaact ctatacgaca gttcgcaatc catcctgtcg ttaaacaaaa ctaggacgca
     1861 ttaccatcat tggtgcagtg cagacgggat tgtttcgaca gccccgtctt tttttatttg
     1921 ttagaaaggt gtaatataca atgtataact ttgttctcaa tacccggcgg atgacctttt
     1981 ctgctttggt catcgccatt tatatcgccg tcttgtatgt aacccagggc atttccttcg
     2041 gtccttacca aatccggatc gcaacggctc tctacggact ggcgtttctc tatccttttt
     2101 tagtcgtgcc tttgggcatt gctaatttta tcgccaactt tctctttggc ggactgggcc
     2161 ttatcgatat ggtcggcggt tgtgccgtcg gcatgataac gacctgg
//
LOCUS       DAWDXZ010000307         2195 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9300_length_2195_cov_6.213084, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000307 DAWDXZ010000000
VERSION     DAWDXZ010000307.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9300_length_2195_cov_6.213084"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1681..1690
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          497 a          654 c          508 g          526 t
ORIGIN      
        1 cccgaagcct atgaagaaat ggtttccctc atcgaaaagg ccaaggccga cggccattcc
       61 gacgaagaag cccatatgat tgcctttgaa aagaaacatg ggaaaaatgg caagaaataa
      121 gaaaaaagag ctgcctcagc agctcttttt tctatgccca tatgcacctc ccttgcggga
      181 atcggactcc ccttgccccg ctattagctc tcgtgtatac ttaaaggata atatacacgg
      241 caaaggaggt cttagcatga ccaaaacgca gcatggtatc atagcttgtg tcatcggagc
      301 agtgacgtgg ggcgttaacg gcgtcgccag tcagtttttg ctctccaatt atcccgtaga
      361 accctcatgg gtagcatcga ttcgcatggc tctggccggc cttctcctaa cgttcatggt
      421 cataccgaaa aaaagccatt taattcgaca gatgctgcat gacccgatca gtctgcggca
      481 tttggtcatt tttgcgattt tcggtctcac cctaagccaa ttttcctatt tatcagccat
      541 aaaaaattcc aacgccggca cggcgacggt cctgcagacc ctctgcgtcg tctttatgtc
      601 cttctactta gccgttcgct tccataagcg gccgacgacg agagaaatca tctcggtctt
      661 cctggccttt ggcggcgtct acttatcagc aacccagggc aacccgtcga cgatggtcct
      721 ctcgcccctt ggcctgacct ggggcctcat gggcgccgtc gccggtgtcg cctatcccgt
      781 tttatctcaa ggcctggccg cccactgggg tgccctcatc accaacggcc tgggcatgat
      841 taccggcagt atcgtcttag gcctgtcgac acaggtttgg acccactggc cggccctcga
      901 cgctatgggc tggtttgccg tagccttcat tgtcatcgtc gggacggcca tttcctttac
      961 ggcctttgtc accggcatca gtgaaatcgg ccccatgaag gctaccttaa taggaacctt
     1021 ggaaccggtc atcgcttcgg tgacctcagc cctcgtcctg ggaacgacct tccatcccgt
     1081 cgaactcgtc ggctacgcct gcatcatcgc aacggtattc atcatcatcc tgcagaaaaa
     1141 aacgccggct gagtctattt cgtaatacgt atttttcgtg tataatagac catgtattga
     1201 taatctatct gctttctttt gaaaggtgtg atttatatga tcattgacga cgccggcggc
     1261 ctcgtttttc gtccgcccag tgaagcccgc agctttatcc tgcgcgtaac catcggctgt
     1321 tcccataata cctgtcgttt ctgcaatatg tataaagcct cgaaattccg catccgtccc
     1381 ctcgaagaaa tcgaagggat catcgaacgc ggtgctgaag ccctgccttt tattcggcgc
     1441 atcttcctgg ctgacggcga tgccctggtg ctgccgaccg ataccctagt cgccatcatg
     1501 gaaaaatgct acgaaaagtt ccccaagctc acccgtatcg gcgcctatgc gacgccggcc
     1561 gatttaatcc gcaagacgcc ggaagaattg aagcgcctcc gcgaagcggg ccttgccatc
     1621 gtatacatgg gtatcgaaag cggtgacgac gacgtcctga cctatgtcga caagggaacg
     1681 nnnnnnnnnn taccatcgaa gccggcaaaa aagccttagc cgccggcatg aagctgtcga
     1741 cgatgattct cctcggcctc ggcggtcagg aacggacaaa agagcatgcc ctccatacgg
     1801 ccaaagtcgt ctcggccatc aacccgacca tgctcagcgc cttgtccctc atcattcccc
     1861 atgaagtccc cctctacgac gatgtcataa gcggtaaatt cacgcccctt acggcccggg
     1921 gattcctcaa agaattggac atgatcctgc gccatacgga aatgaccaat ccctgtatct
     1981 tccgcagcaa tcacgtatcg aacctcttcc ccgtcggcgg caccctgccc atggacaagg
     2041 aaaagatgtt ggccgccttg gaacaatata ttccacaagc cgacgatacg cgtcccctct
     2101 taaacgataa cggcaatttc taatctgacg ggaggtgcct tcatgaaaaa aatatttctc
     2161 cttctcctgt tcttcctgct gacccttacg gtcgt
//
LOCUS       DAWDXZ010000308         2191 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9313_length_2191_cov_8.930712, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000308 DAWDXZ010000000
VERSION     DAWDXZ010000308.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9313_length_2191_cov_8.930712"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          509 a          577 c          573 g          532 t
ORIGIN      
        1 ttccattctg agaccccttt tttgtatctt tttgaaggga agcccttggc tgacaggaag
       61 ggtcccgata cttcggtgcg ggaccttttt ggcgtagaga aaactggcct ctgagcctca
      121 aaaatgataa aatgtatagg aaaataatcg atgttactta tcataaaggg ggatttttta
      181 tgtttaccta ttatatgccg acgcaggtgt ttttcgggaa aaactgtatt gccgagtcag
      241 gtcaggtgtt agccggtttg gggaaacggg ccttattggt cacaggccgt cattccgcca
      301 aggtcaacgg ctctcaagat gctattaccg gaaaacttga tgaattaggc attgcctggt
      361 atctctttga cgatgttgaa aacaatccct ctatcgatac gatccgcaga gccgcatctc
      421 ttgccaagga gaagggtgtt gacttcgtcg tcggtatcgg cggcggttcg cccatggatg
      481 ccgctaaggc cattgccctg ctctgcacgg atgatctcga cgatgaacgg cttttcaaag
      541 gaccttacaa aaagcccttg cccatcgtgg ccgtgccgac gacggccgga acggggagtg
      601 aagtgaccaa agtcagtgtc cttaccaatg ttcatgccgg gaccaagcag tccatcaata
      661 cgccttttat ttttccggtc tatgcctttg ccgacccgac ttatacggaa agcgtgtccc
      721 agaccgttac catcgacacg gccatcgatg ccttgagtca tgccgtggaa ggctatatgt
      781 ccaccatgtc gacgcccatg agcgacgtct gggccgaaga agccatgcgc tttatcggcc
      841 tccatctgac cgacttgaag ggggatattc cctatgccgt ccgggaagat ttgctctatg
      901 ggtccatgct ggccggcctt accattgccc agacgggaac gaccatggtt cacagcatgg
      961 gctatatgct gacctattat cgcggcatca ctcacggccg ggccaacggc ctgcttttgc
     1021 aggcttacat gaaactcatg gaagaatcta tggccgataa ggcggaacgc gtctgggcca
     1081 tcctcggcct ttccgggagc gaagactttg cagcccttat gacgaatctt atgggcgata
     1141 cggtgggact caccgatgat gaaattaaaa agttcgtcac catgaccctg ccgaccagcg
     1201 gtattcagcg aacgccttat gacgtaacgg ccgcaaattt gacgacaatc tacaaagggc
     1261 tgtaatcgga ggccgcctga ttcaatatag gaggaatagt atggcagaat tattagcgcc
     1321 ggcaggctct ttagataacg tacttacggc cattgatgcc ggtgccgatg ccgtttattt
     1381 tggcggcaag agctttaatg cccgccgctt tgcacataat ctagacgatg aagaaatcgt
     1441 ccgagccgta agaacggccc atttatttgg cgtaaaagta tacgtcacgg tcaatattct
     1501 catggccgat acggaactcg atgacctgac aatctatttg aagtcccttg ataaggccgg
     1561 tgtcgacggt atcatcgtcc aggacatggc ggtggcactc ttagctcgcc gcgtcgtgcc
     1621 cgacctgccc cttcacggca gtacgcaaat gaccgtggcc gattctgacg gtgtccgttt
     1681 cttggaagaa aacggcttca cccaggccgt cttatctcgt gaattatctc ttgccgatat
     1741 acagtcggtc tgtgcttcgt cgcctcttgc cattgaggta ttcatccacg gggcatcctg
     1801 catggcctat tccggccagt gcctgatgag cagcttcctc ggcggccgca gcggaaaccg
     1861 cggggcttgt gcccagcctt gccgtcagcc ttatacttta gttcgcgacg gtaaagcggt
     1921 catgaaacaa gaagcctatg tgctcagcct caaggattta aaaggccttg attatatcga
     1981 cgacctcctc gatgccggcg tcacgtcctt taaaatcgaa ggccgcatga agggagacac
     2041 ctatgtcagg accgtcgtca cggcgtaccg caaggtcatc gacgcccata acagcagccc
     2101 gaaagcccgt aaagaggctc atgatgaagc ggcagcctta ttgacctcgg cctttaaccg
     2161 cagctaccaa gacgattttt taagagggat g
//
LOCUS       DAWDXZ010000309         2190 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9317_length_2190_cov_9.691803, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000309 DAWDXZ010000000
VERSION     DAWDXZ010000309.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9317_length_2190_cov_9.691803"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          693 a          538 c          432 g          527 t
ORIGIN      
        1 cagcatgata aggcctttat ctgaagcccc cttatagaag gtataccgtt catagaggaa
       61 cttggtcagt ttagccggga aggtcgtcgg cgggcaggct tcgaatttat cggcagcgtc
      121 aaagacaatg ccggcttccg tcgtattgga tacgacgaat tcgagggcat cttcctgagc
      181 caaagtcatg aaggcgtcgt attcttcacg ggcactgaca atagtattta ctgaagtaat
      241 gacccggttt tctttacaga tttcgccgtg ctgctgacct ctcaggcaga cggtgtaaac
      301 gtcgttttgt ttagaaaagc ggtcggtctt gcccgcacgg ggcatgacga cagctacgct
      361 gccgtcaaaa ccgttttcct cgttagctac gtcgacggca tagtcaacga aggcgcgcag
      421 gaaattacct tctccaaact gtaagatccg tacgggatgc tgttgtttct gatatatttc
      481 acttacattt ttcatatggt ctgtctccat cctaaaaatc acttaatttc tcttcggtaa
      541 cggttcaact cttccaacca gtcctaaata agacaaggca cctaaaatac agagcccgcc
      601 ggcaacggct aagggaatta aaaaggatcc ctgcgtcagt gataacatta caccggtaaa
      661 cgtagtaata aaaatcccgg ccagatttcc ggcaaagttc ataatccccg taatagaagc
      721 aacatgcttc ggtgtcggag caatttcacc gacaatcgca aaggtattcg caccggcaaa
      781 ggataagctg gcataagaaa gagaaaataa agctaagcaa acccaaacat tttctacaac
      841 ggcacataag gcgatacaag aagatacaag catacctaac actaagcaga attttctgga
      901 aaacgtcata gagtgtccat gtctcaagag aaaatctgat gtaaacccac cggaccaact
      961 acccaatata ccacacaatg gcggaagcat ccccagtgtc cccaatgctt ttaatgaaaa
     1021 gccgcgtgtt tccatcaaat aacttggaaa ccaggtaata aagaaataga tagcaaaatt
     1081 cagacagaaa aggccaatca taagccccca aatagtccga taatggaaaa gatccagcca
     1141 agaaatttta ctttctgttc cagtttcttc aatactggtt cctctatcag ccaataaagc
     1201 gtccacctgt ttgggagcaa tatctctaaa tttttccgga tcccgataaa taaaccacca
     1261 taaaatagcc cagagaatac cgattgcgcc ggtaatcatg aaagaaccgc gccaaccaat
     1321 catagcaata ataaaagcaa tgattggcat cgaaagagcc gagccaactc ggttgccgct
     1381 gtcaaagata gcacaagcta tccctcgttc tttatgaggg aaccagttat aagcagtttt
     1441 acaaaaagac ggaacagctg cagattcacc gatacctagt aaggctctgg cacccagcat
     1501 agctccaata ctatgaacca accccgtaat ggctgtaaaa acagaccacc aaccaacggc
     1561 tgcagctaag ctgatacgga cacccacttt atcaatgaac caccctgccg gcatttgaca
     1621 taaagcatag gtccaaaaga aggcacttaa tacgacaccc atcgtggcag catccatacc
     1681 taattccatt cggatatgcg gtgctgcaat agcaaggtta gctctatcga tgtagttaat
     1741 tgcgttagca agaaaacaca taccaataat aagccagcgc atccgactaa atctaacaat
     1801 catcgtaaat cgcctccctc tttaaaattt aatgacaact ttcaacatat tggccgcatt
     1861 tttatcgaaa tcttcgaaag cctgcggagc attatctacc gtgtattcat cggtaataat
     1921 tttttctaaa tcgacagccc cactctttac cagatcgata agttctaaga agtctttttt
     1981 cagggaatta cgggaaccaa aaatgttcag ttcttttttc tgaatcaagg tgaaattaaa
     2041 atcaaggttt tgcttgccga caccgatcaa tacgacttta ccgccaaaag cagccgcatc
     2101 aatgcagttc tggaaagtgg acggacgccc aacggcttca acggccacat caaagccttg
     2161 ctgattcgtg acttccatgc agcgttcaat
//
LOCUS       DAWDXZ010000310         2188 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9325_length_2188_cov_7.977496, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000310 DAWDXZ010000000
VERSION     DAWDXZ010000310.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9325_length_2188_cov_7.977496"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          472 a          600 c          576 g          540 t
ORIGIN      
        1 gtcatactta cagcctggcg tcatgacgcg agccacgccc gccgcgaatg atttccgggc
       61 gacggcacgc gtgtatgcgg tgtccgcggc gccaaggtaa tcgtgtagca cggtttccag
      121 gcggcggacg ccgtcccact caagcgcctg gaagtagtcg cgcacctcgt tgaaacggtg
      181 ctgctccgat accagcatca gtgcggcggt aatcttgtcg cgtccggtaa tgccgtagcg
      241 attttcgagg taccacgcta tcccggcatc atcggtatcc tgccacagtc gccggtcctg
      301 gcagccgttc cagggtaagg gaccaaggac gaggccgcgc gttgaaaaat catcgatagc
      361 gaccttgccg gcaagctcag ggtcatagtt aaggatgcgc gttccatcca gttgacgtca
      421 tcgttttcga ggacgatttc aggcgttccg gcagcaggtt cgaccgctga aaagacatcc
      481 gacgcatgct gctgggccga ggcgttaagg tccgtcataa cggcgctgtc ctgcatagcc
      541 aggcttttca tagcctgata gctgggcagg cggtttaccg gcgttccgtc cttcgcatcg
      601 gcgtcgtcgt caccaaatcg atgcaaccgg acaagatcaa acgcgttgac caactgaccg
      661 ccgcatggat cggtggcgtg atgactgtat aaaaatttgc cgtcgtcgta gaggacagcc
      721 cctgcgacag tagtaccacc ggtatacgtc aaacggtcct ctttgtcggt ctcctcatac
      781 gcattaggta tataagcctg gatagcctgt aggatgtcat aggtacggca gaaggcgcct
      841 acgatcccct ttttggccaa ggggtcctgc tgtttggcga gtaattcccg cggtttggtc
      901 tccttgccgg ggacctgtgg ccagaatctg acgtctcgcc agtctccgta ttggtctaag
      961 atgccctggc catctgcaaa tggcttatcc gcatatcgga taatgtactg gctatcggcg
     1021 ctgcatccgg gccaatacat cagccgggaa acgtcaaacg tcgtcgggtc gcacagctcg
     1081 atgccgatgt tcgcggccag cttccgggcg atgggctcgt actcgtccgc cgttacggtc
     1141 tctgccagtg ggatgataac gcgaagacgc gggcggaact cggcatgtga ccgggtactg
     1201 tagatagcgt aggcaatgcc taaaccgtct acccggcgga tgacgttttc cgtctcgccg
     1261 cttgcgatgt tatctaggtc cagtgttacc aggtcgcgcc cggtaacggc tgaggccttg
     1321 cggcgggtcc cttgcaatgc cccgccgaca aaaccgccga tgtccttaag gtcaccttgc
     1381 tggcgcttgg acaatcgcat atagtcgtcg aatgtttccg gggttcgttg cggcgtgcgc
     1441 agccggtcga taaactccga ccacatgacc tcagatggta tccaattagt tgagttacgg
     1501 ctgttaccga tgctgataat cagcttacga tcatattgca aggtaacacc tccttaatgc
     1561 gcgatccatt atcccttaat gagccatata atcccgtagc cgatagcagt cagtactacg
     1621 aggctgtata ggataagaat ccaccataca atcattctaa gcatagttga taccctcttt
     1681 cataagctgg agattgactt gataaaatat tccttgacag cggccttttc ttcctcgccg
     1741 taggccgtgg ccaaggatgc tacggcgtgc atcaaatgag tggcgatgta ataggcgttg
     1801 ccgttgatgg attccagact catgtcggcc gctttgatgt tagccagcag gatgacgaca
     1861 tcgttatcgt ctaaaaaatc gcgcccttcc aagttatccg ccaacaattt aatgcttgaa
     1921 atcaagttat cgttattcat tctcgtggcc tcctatcaca tttccatcgc agcagccgtt
     1981 atcagactct ttaatgcctt tttttcttct tcggagcatc cgtccataat catacccatc
     2041 atttctaata atttgacaat cgtcgggagc gttggcccgt taatgcttcc ggtacggtca
     2101 tacccctcaa gatcggctaa taatattaca acgctatcgc cgttaaaatg aaaattagtc
     2161 aatgcgtcgg ctaatgtatc aatgtgtt
//
LOCUS       DAWDXZ010000311         2160 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9458_length_2160_cov_6.209976, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000311 DAWDXZ010000000
VERSION     DAWDXZ010000311.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9458_length_2160_cov_6.209976"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          559 a          506 c          608 g          487 t
ORIGIN      
        1 tgcagaatac tgccggcgcc acaacgatgc caacattctc accatgggcg gccgtgtcat
       61 cggtccggga ttggccatcg aaatcgtccg tcttttcctc acgaccggct tcgacggcgg
      121 ccgtcatgca gaacggctca atatggttaa ggaaatggat acgtaaagga tctgacgaca
      181 gtgattaata aaattggagt attgacaagc ggcggcgatt ctcccagcat gaatgctgcc
      241 atccgcagcg tgactcggta tgccatctat aagggcctga cggtggaagg catacagcgt
      301 ggctatgaag gcttgatcca cggcgaaagc ttggctttga atcgtcgctc tgtaggcggt
      361 atcatccatc gcggcggtac gcatttacgg acgtctcgtt ctgaagaatt catgacccga
      421 gaagggcggc tgaaaggcta taactggctc gtgcagaatc acatcgatgc ccttgtcgtc
      481 attggcggca acggctccat gaaaggtgct gaaaagctga gtgaaatggg gctgccgacg
      541 gttacgattc ccggcactat cgataacgat atggcaggaa cgcaatatac cttgggctat
      601 gatacggctg tcaacaccgt attagaggca gtgaaccgaa tccgggatac ggctgtttct
      661 catgaccgcg tggccatcat cgaagtcatg gggcgccatg caggcttcat cgccttggaa
      721 tcgggactgt cctgtggggc tgaaatcgtc ttgataccgg aaaagaagat gtccatggaa
      781 gaagtgtccc accatctgga agcatctcat aagcagtgga aaaagaacag catcatcatt
      841 acggcggaag gcgctgtgag cggagccgag gtccttcagt atatgaaggc gcatcatccg
      901 gaaatgaatc ccagcctgac cgttttgggc tatctccagc gcggaggcgc accgagtgcc
      961 cgcgatgcga agatggccag cctttttgga cagaaggctg tagaatatct gctgtctggc
     1021 ggtacggggg ccgtcgtcgg tcttatccac gacaacatcg ttgccacgcc ttatgaggaa
     1081 gctggccaat ttcagttcgg tatcgatgaa aacgaatatg aattggtcca tgcactggga
     1141 atgtaatagg aaggtttact aagaaggggc ccttctttta aggcaaacct ctcaaagtaa
     1201 cgaacaagag gagccttgtt gtataggtct cctcttcttt ttatgtaccc cttggaggag
     1261 gtaatttcgt atgattacgt taatggggaa aagcgtcttt cgcggcatca ccattggacc
     1321 gctggcactt tttcatcgaa acacgatatc tacagctgtc catgaaatag aaagtgttga
     1381 agacgaagtc aaacgctttc atggagctag agaaaaggct gtggaacaac tgcgcatgct
     1441 ctatgacaag gccgttcaaa aggtaggcga agagcaggct gccgtctttg aagtccatca
     1501 aatgatgctg gaagatgacg actatatcga atccattgaa ggactgattc aggataaaaa
     1561 tatgaatgcc gaagctgcgg tagaacagac ggcccggttg tttgccgaaa tgttccgctc
     1621 tatggaggat gcctacatgc agggccgtgc ggccgatgtc ctcgatattt cccgtcgcgt
     1681 acagatggaa ttgtgcggcg gtcagggcgt cgatttcagt aagtatggtg aagtcattat
     1741 tgcagccgat gacctcgtac cgagcgaaac gctccagctc gatacggata agcttattgg
     1801 cttcgtcacc tccggcggaa gcacgagctc gcatacggct atcctggccc gtacactggg
     1861 tattgcttct gtcgtaggca cgggaacaca gcttcatcag gacgtagacg gcatgttggc
     1921 aatcatcgac gggactacgg gcatggttta tatcgatccc gatgacgaca ccctggaaaa
     1981 gatgaaggcg aagaaggctg aagaagacga acggcatgct aaattagaac aagtacgggg
     2041 acttgaatcg gtgacccttg atggcaaaaa aatcaatgtc tttgccaata tcggcaatac
     2101 ggaaaacctg ccgcaagtgc tccaaaatga tgcggaaggc atcgggctgt tccgtagcga
//
LOCUS       DAWDXZ010000312         2153 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9477_length_2153_cov_7.465682, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000312 DAWDXZ010000000
VERSION     DAWDXZ010000312.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9477_length_2153_cov_7.465682"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1259..1268
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          652 a          475 c          414 g          602 t
ORIGIN      
        1 tccttatttc acatatccaa ataaaaggat gggaacacgg accggaaggg acgaaccttc
       61 atggggttca aagcattact tggtttatca cacgtgtaaa taattgagat tcatttctcg
      121 tatgcttttg ttgcctacat cgtatcatcg ctgctatgcc tttgtaaaat acatcgattt
      181 catccctgta atgaataaat ttcacgtaaa aaagcacctg actccgaaga gtcaagtgct
      241 aaaaaaatca gatgtgcagc ttcgattttt gaactaaatc caaaaagagt ttcataggtg
      301 tactcagaaa tttctcctga tggtacgccg ctaacggaat ccaaggaagg gacggattac
      361 tgagacgcag ggcttttact cgcttactgc agaatttttg aatgatcggc ttcggtaaga
      421 tggtgactcc ctcgccatga gaaacggctt ctaccaataa gtcccattgt gcgctctcca
      481 tttgaatctt aggcgtgaat ccggcttcct tgcaagattc caccatgcgg tcgtgaagca
      541 tgaaagattt gtcgagcgaa aaaaacggct ggtcctttaa ctgggaaaaa gaaacggtat
      601 cataattagc caggcggtga ctcgttgaga cgacacaaac cgcttccgac tccataatgg
      661 ggattttatt gacctccgga tggtcaaaag gctcgatgat aataccgata tcgacggcac
      721 cggcctgtat cagtgaaaaa acggtttggg ctccgatttc cgtaatttgc agggtgattt
      781 cgggatacat ctgctgatat tgatgtaaaa gcgaatgaat gaccgttacc gtaacaggcg
      841 gtatcccgag aatcaacttt ccccgctgac tgtgcaaaag agtcgccagc acttccgttt
      901 cagcctgata attcgatacg attttgacgg cactgttgta aaaaatttcg ccggcagatg
      961 tcaatttaaa cttacgagcc ttacgatcga tgagttccgt attatacgtc tgttccagat
     1021 tttttatggc cttactgatt gtcgattggc tgataaagag agattccgca gctcgggtaa
     1081 tactgttttc ccgcacggtc tcgataaaat aggaaaagtg gttgatgttc ataagcatgc
     1141 ctccttctat ataaaatatg tcatcaaatc attttttgac aattccccat ccgaaaagcc
     1201 ggcatcagta taaccgtcga cgcaaataca atcatcagct ccatgaaaat agttcatcnn
     1261 nnnnnnnntg aaggctgtca ccgagatgac agccttttaa tcagttttca agggcttcta
     1321 tcatcgactc gaaagcaggt acataatagc gggtaatccg ctgcaccttc gcctctacag
     1381 gttccgtaca gtccggacgg taccgatctt tcaacatgga cagccaaacc gattcatcga
     1441 caaagagatg ccgttcgtaa gcatccaaaa tcaattcctt cggggtcaac gaaatggcaa
     1501 gcgagggatt actttccgaa agataatcgc gaaacacctg ctgtcccaaa tcaaactcat
     1561 ccataaacca atgaacaatg ccgtgcaaaa taaatacatt cgacaaatcc tctttctgta
     1621 aagaagaaaa atcgttcaag ccttgtgaaa acagatcaat ggttttcata gactcaccac
     1681 cttttaacca tcttatatac ttatcgtata aaataaaggt tataattgca aaagacttta
     1741 attcatcagc ggctatactt tttttcatcc atatgaccca tcttgaatcc ggaaaaatcg
     1801 aagacctatc cctccattcc attttcatcg gattatccat gacgacatcg caagtgataa
     1861 agcaacaata gtaattatac cattaatcac tccctatgta tttgcataaa ttaaaatttc
     1921 aattgcgatt tatttatgag agcgtagtcg tcatttaata gcaacaccat aagattgtct
     1981 aaaaaacctc tttatctgca atccttatgt atatctttat atggtccggg tagacgcctc
     2041 catctagtga gcagaaaaaa tagttccaaa atcaaaaggg gactccaaaa atcggagtcc
     2101 ctttttgagc tttacctatt caatttatac acttggcgtt acggtctatc agg
//
LOCUS       DAWDXZ010000313         2150 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9492_length_2150_cov_8.809069, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000313 DAWDXZ010000000
VERSION     DAWDXZ010000313.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9492_length_2150_cov_8.809069"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          517 a          585 c          566 g          482 t
ORIGIN      
        1 tctcttagat tctatcgtgg ccgaatacga ttcgtacggt gacttcctcg atttggaaga
       61 tggggttctg cccattcgtc ccttgattct cggcggtgac gacattacct ttatttgccc
      121 ggcacgactg gcaattacgt atacgaaacg cttcatgacc accttgaacc aatattttgc
      181 ctcctgcgcc ggcattgcca tcctgccgac ggcgtatccc ttcttccgcg gctacaaact
      241 ggcggaacag gcctgcgatg cggctaaggt tccgtcgcgt gggaaaaaat catcgcaagc
      301 atcgaaaaaa gaagaaactc cggaatcgtc ttgctggttg gatttcgtca tccttcatgg
      361 cgaacaggct ccgaaactgg atcaaatccg ccagaccgaa tacaagggcg cccttggcaa
      421 catgcatttc ggtccttatc gcgtcgatga cgacagcaac gaacatgcct tggcaaaact
      481 cctttcctgc accaaacaat tgacgacgat gccgtccaat aaggtcaagg aactgcggtt
      541 tacggcacag aaagggaagg ccgatattcg tctctatttg gaacagttga agcatatggg
      601 ccttggcctg cctcatgtcg acggttggga acgctttgaa gaggccgtat ggaataaaca
      661 ggatggttct gcggccgggc agacgcctta cgtcgatgcc atcgaaatca tggattacac
      721 tgtcccggag ctgcaatagg aggtacggac tatggaatat gaagtacgga ttaaaacgga
      781 atcgccgctg tgcctgaatt ccgggaaggc tgacgtcaat gtcgatgccg acatcgtcca
      841 tgatgcactg ggcatgccat acttcccggg aaaacgcctg cgcggactcc tttatgaaag
      901 tgccgtcgaa gtcattgaaa tggcggaatt atgcaccaca ccttttatcg aacgaaagac
      961 cgtagaggaa ctgttccaca aacgggcgga cagcacggtc gaactgatcg tcccgagttt
     1021 ttacctggaa gactatcgga acatggccga agaatggaag tatctccagt ctcattttga
     1081 tctcatccaa ccggaatcgg tcttgcagac ctatacatcg attcgctttc agacggccct
     1141 caacgccgat gggacggcca aagaaacgtc cctgcgcaat atccgcgtcg tcgacagcga
     1201 tttggtattc gttggaaaag ttcaggtcaa cggcggcgaa cctcgccatg aacaagcttt
     1261 ggccttggcc gtttccaatc tccggcgggc cggcatgaac cgcaaccgcg gctttggacg
     1321 cctctcttgc acgatgaaga aacaggaaga cctgatggct caggttttct cgaaaggggg
     1381 tcgtaaatga tgcagatttc ttacgttacc attgaaacct tatcgcccgt cgtcgtgacg
     1441 gctccgggca gttcacagct cctcacggcg agcagcgagt ctttcagcgg aaccctcctg
     1501 cgcggcgtct tagccggtaa atatgtgaac gatcataatt tggggacgaa ggcccatgag
     1561 gatgaaaatt ttatccgcta ctttttttcg gaccttcgct tcgtcagcgc caatcccggt
     1621 gtcgacggaa aacgggctat ggtgctgccc ttatcgctta tgaaggcaaa agccgtatcc
     1681 ggcgatacca agccgattct cgacttgctg tccgatcggg gccaagccgg atataaaagc
     1741 cttaaaggcc tgtccgtcgt cgacggcggg aagatttgcc ctgtgtcggt caaatcgagc
     1801 atgaccttcc acatgagccg cagcggcgat aaggaacgcc tgagcggcca cagccaggac
     1861 ggcaaggtct atacctacga atctatcgac gccgatcaaa ccttccaagg tgccgtcatc
     1921 ggtgatgacg attgcttgca gtccttcttt gaagacgtcg gtaaaaaggg aaagagcttc
     1981 aactgccgta tcggccgctc caagcggacg gaatacggtg gctgccgcct gacctttaca
     2041 ccgccgcaag atattcccga cgaagccttt tccgaaacga catgcctcgt cctcgataca
     2101 ccgctgttac cgactggagg ccttgcggca agagctgacc gggtactgca
//
LOCUS       DAWDXZ010000314         2110 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9660_length_2110_cov_6.272019, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000314 DAWDXZ010000000
VERSION     DAWDXZ010000314.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9660_length_2110_cov_6.272019"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1662..1754
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          514 a          464 c          572 g          467 t
ORIGIN      
        1 tcgtcgatga ggaggacgcc gtactggtca cagagtttgc gcaggcgccg taaatattcc
       61 ggcggataga tacgcatgcc ggcagcacct tggagaatcg gttcgacgat gagggcagct
      121 gtttccttac cataggcggc gaaggccttt tcggcaccga taaagcattc acaatcgcag
      181 ttctcgcgag ttttgccata gggacagcga tagcagtcga ggccatcgaa gtgaatgtta
      241 tgcatcatca ttggcttata catttgagca tagaggtcca tgctgcctac ggacaaggca
      301 ccaatcgttt cgccatggta ggattcggac aaacacataa agcgctgccg tttcggatgt
      361 cccttctggt actggtattg gaaagccatc ttcaaagcac attcgaccga cgaggatcca
      421 ttgtcggtaa aattgaaatg agtcaacccc tttggcagca agggcagtag ggtttgacac
      481 agttcgatgg ccggacgatg agaaaagttg gcaaagataa cgtgttccaa tttgtcgagc
      541 tgatccttga tggcgtcgtt gatttcttca ttggcatggc ctaagagatt gcaccaccag
      601 gaactgatga tatcgatata ttctttgccg ttcgtatcgt ataggtagca gcccttaccg
      661 tggtcgatga cgacgggcgg gaatacttcg gcatctttca tctgctgggc cgggtgccag
      721 atatatttta aatcggcttc aagtaaattc atgtaaatgt cctcctcatt ggtataaatg
      781 gatcagctgg tcggtatcgg cgtcgatgat ttcgtcgtca tcggagacaa cggctaatac
      841 cggcttttgg ctcaattttt caatcatgcg gatattgtct ttttccatgg ggcttcctgt
      901 ataatgattg acgatgaagc cctgaacggg gagatgcttt tctttcatat aaaaggccgt
      961 taagacggct ccgttaatgg taccgaggcc ggcggggcag acgatgaggg acggcaggtt
     1021 cagccagcga atgacgtctt ccaactggaa aaaggcctgt tcatcgtcgc gaaggggaca
     1081 gataatgccg ccgctccctt cgacggtgac gtagggataa cgggccgtta ccttttgcca
     1141 gccgcgcagg atgacttcct tttcaatggg gcgaccttcc cagcgggcgg ctaagtgagg
     1201 cgatacggca tggctgtaga ggtaggataa gagaagctcc gaatcttctc cgatgtcggc
     1261 cgttttattg acgaaaccgg cgtcactgtc ggcgacggtc ggagctccac tgatggcggc
     1321 tttgtagtaa ccgacgtcgt agccgtgttg acgcagcgtc ttgactaaga gggccgttac
     1381 ataggtcttg ccgatatccg taccggtccc ggtaataaat aatcctttac tcatggtgaa
     1441 ttaactcctt tctaacgagg aaggcttcgc tgtatttgac gggtcagaaa ggccgcgaca
     1501 atacataaaa agatgtcgcc gggaatgggt aataagaaac agtaaacgaa taaggtccac
     1561 aagctgatgg gggttcccag gtagaggttg tttatcatgg ttaagtaagc catgccggcg
     1621 gtgtaaatga cgaccaggcc ggcaaagtta gctatgagga gnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnctttgg tgagccaaat atcccgtcaa tccggctccg ccaatgaagc
     1801 ccaggagata gccgaaggtc ggttgaaaga tgtaagacgg accgccgcct gacgtaaaga
     1861 ccggcagacc gactaagcct aagagaacat agacgccgac ggaagcggca ccgcggcggc
     1921 ctcctaagat ggccccggcc agggtagtga acaataattg cagggtaaag ggacagaagg
     1981 gcacgggaat tcggataaag gccccaatgg caatcaaggc tgtaaataag gcgaatagaa
     2041 ccaagtcccg cgtagtacag cggcgtgcag acaaggctga tgaagacatg aaaccactcc
     2101 tttttgtata
//
LOCUS       DAWDXZ010000315         2107 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9674_length_2107_cov_8.293372, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000315 DAWDXZ010000000
VERSION     DAWDXZ010000315.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9674_length_2107_cov_8.293372"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          531 a          439 c          587 g          550 t
ORIGIN      
        1 aatggccgac cttttccttc aagggattgc tcgtagctgt gttgcagctt ggcatgtaat
       61 tcgctatcgg ttagactgtc ttcggtttga ggaatttcgg gaagcgtaag ggaaaatgga
      121 atcccatggc tatagataat ctgacgatac agggcgttga tgacgacgga aaccgggatg
      181 ccaagttgct gaagaatgga ctcagcctct aactttacgt tattttcgac acgggcgctt
      241 actgttgagt ctttcatgat gaccacctcc tacttatagt gtaatgcatt gacttgcaaa
      301 ttgcaataca taaaaccccg aaggatgaat ggttttacct aaaaatggaa ctcctcttat
      361 tttaaagaaa tacttcgtaa aatataaaac tatatgattt tatgcgtgaa tttaattcaa
      421 aaagaaagtt tggaatgatt ttatatatat tttttataat ttttttaaat atatcttatg
      481 aagtttatgt acttatgata aaatggaaag tagtggggct catctattat atcttgttat
      541 aaatagaaag gatagaaaga catgattaaa gtactgttta tttgccatgg aaatatttgc
      601 cgctcgccga tggccgagtt cgtcttccgc aagatggtcg atgaggcggg gctctctgat
      661 tcgattgaag tcgcgtcggc agcgacgtcg gacgatgaga tttggagcgg cagcggaagt
      721 ccggtctatc cgccggtgga acggctgctc aaggaacacg gcatggaccc caaggataag
      781 cgggccgtac ggctcactgc cgatgattac gacaaatacg attatttcgt gggcatggat
      841 gatgccaatc tgtccgacat gaagctgact ttgaacggcg acccgcagcg caagtgttcg
      901 ctcctcctcg attatacgca ccatccgcgg tcggtggccg atccctggta cacccgggat
      961 ttccagaaga cctgggatga cgtcgatcac ggctgtcgag cttttttgga atttttgaag
     1021 aagcgtcatt tttaagagac ctatgagtct caaggggtac actttgttag gagatgagga
     1081 atatggccat tgatgtcgac tctcttttgg ccgtattgga agatccggcg ccggaagaat
     1141 gctgggtcaa agccgagcct aaaaagggcg atcatatccg cgtgcggcgc atcggcggct
     1201 tatattacca tcacggtatc tatgtcagtg acgacgaggt catttctttt gccggcgacg
     1261 acgattataa tcccgtcgac tggtgggata cggagataca ggctacgtcg tttgagcagt
     1321 ttcgccaggg tggcgaaatc gaggttcggc agtatacgga aaaggaacgg gaaagcctct
     1381 gtgacgtcga cgatacgatc gcctttgccc gggcttgttt aggcaacaag gactatgact
     1441 tgttgttcca taattgtgag cactttgcca atgcttgtaa gctcggcgtc taccgcagcc
     1501 agcagacgga gaacctcctc ttgggggatg ccttgaccaa agcaatccag tgcctgtggg
     1561 atgaagaggc ccgccggcag tggtttactg aggcctggca ggatctaaag gctcaggtag
     1621 aggataaatt gcgccgggac aaggaagagg aagaaaatca atagatcgtc tgctcgtcga
     1681 tttggcggaa taagggttcg taacgcttgg catccctttt caggcagacc aggtaatagt
     1741 cggtttggca ttcttcgtcg acgatgggca cattgatgcg ccccggcatg tcctcgccgc
     1801 ggtgcatgaa gtagctcgag acgaagaagg gatagtcgga attttgggcc agctccagga
     1861 aggactcgcg ctggacctgc agtaagtaac gggtgttcgg cgtcttgtct ttggtcatct
     1921 gcatccaaaa gccgatgtgc gataagagca ggatgggccg gccgtccaga tctttgaggt
     1981 ggatttccgg gtaaaaggcc aaggcatcgg atggagggat ggagagaaac agtttttcac
     2041 tgccgcattt tttgacgaat aaatcgtcgc cgtcgggaat cgtatgggtg acggccagtt
     2101 ggtatat
//
LOCUS       DAWDXZ010000316         2105 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9682_length_2105_cov_6.050244, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000316 DAWDXZ010000000
VERSION     DAWDXZ010000316.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9682_length_2105_cov_6.050244"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    708..717
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1582..1591
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          513 a          514 c          525 g          533 t
ORIGIN      
        1 acctttgtca tcaacccgcc gacggctatc tttaatacct ggttgttcga cagcctcaac
       61 agtatgacga cgtcgagccg tatcgtcctc ggtttcgtcc tcggaggcat gatgtctatc
      121 gactttggcg gtcctatcaa caaggcagcc tacgtctttg ggacggcctc gctcatgagt
      181 gctaccggtc aggctgtcag ctcgggcatc atggcttcgg tcatggccgg cggcatggtt
      241 ccgcccctgg caatcgcctt ggctatgctg atctttagaa ataagtttac gccgaaagaa
      301 cggcagtcga cgattaccaa cttcatcatg ggcctgtcct tcattacgga aggtgccatt
      361 ccttttgcag cctctgaccc gcttcgcgtc attccgacct gcgccatcgg ttctgctgtc
      421 gccggctcgc tgtcgatgct ctttggctgc gcccttccgg cacctcacgg cggtatcttc
      481 gtattcggtg tcatcaccaa ttggcccatg tatatcgtct ctttaattgc cggtgtcatc
      541 gtggcagcca tcttactcgg tattttccgc aaaaaacgtc ctcaagagta attgcgtaat
      601 ttccataaat caagtacact atataataga gttttatctc aactattcca aacacttatt
      661 tcctgaagga ggaattttca tgaaagaatt tgaatttgtt gtaaccgnnn nnnnnnnggt
      721 atccatgctc gtccggctgg cctcttggtt aaagaagcta aaaaatttga atccaacatc
      781 agtgtcttca aaggcgctcg taaaggtgat ttgaaaaaaa tcttcaccgt catggcttta
      841 ggggtaaagc agggtgaatc cattaaagta caggtagaag gcgctgacga agaacaggca
      901 gcaagtgctg tagaagcctt cttgaaggaa aacttctaag atttttgagg gggaaagcag
      961 atgctgacgt taaaaggtaa gagtgtcttt tccggtatca gtatcggacc gctggcactg
     1021 tttcatcgca acaccatttc tacagccgtt cggcccattg aaagtgtcga agtagaattg
     1081 gcgcgtttcc aaaaagcgcg tgaagaagcg atgagccagc tgaaagactt atatgttaaa
     1141 gctgtagaca aagtcggtga agaacaagcg gctgtctttg aagttcatca gatgatgctc
     1201 gatgacgatg actatgttga aggtattgaa acaagaatcc gcgaggaaaa aatgaatgct
     1261 gaagcagccg ttgatcagac ggctcaagaa ctcgcggaga tgttccgctc catggaagac
     1321 gcctatatgc aggcccgtgc agccgacgtt ctcgatattt cccgccgcgt agagcagcag
     1381 ctcagcggcg gcccggggat tgatttcagt aactatgatc aggttatcat tgctgctgac
     1441 gacttggcac cgagcgaaac gttacagctc gatactgaca agatccttgg ctttgttacg
     1501 tcagcaggca gcaccaactc gcatacggct atcttagccc gtaccttggg gattgccgct
     1561 gtcgtcagca cgggaacgca gnnnnnnnnn ngtacgcttg atatcggtgc cgataaaaag
     1621 gttgactatt tcgaccttga tccggaagaa aacccggcta tgggcatgcg tgctatccgc
     1681 atctgcctga gccgcgtcga tttgttcaag acacagctcc gcgccttgct tcgtgcttcg
     1741 gcattcggta agattgccat catgttcccc atgattattt ccgtctggga agtacagcag
     1801 agcaaaaaaa tcttggacga agtccgccgg gaattggatg ccaaaggcat tgcctacgat
     1861 aaggatatgg aagtcggcat tatggtcgaa acgccggcgg ctgccgtcat gagtgaagaa
     1921 ctggctaaag aagttgactt cttcagcatc ggttctaacg atttgaccca gtacaccttg
     1981 gctatcgacc gtcagcagac gaaactcgat accttcttcg atccccatca tccggctgtc
     2041 ctgaaactga ttcagatgac cattgaaaat ggccataaag ccggcatttg ggtaggtatc
     2101 tgcgg
//
LOCUS       DAWDXZ010000317         2102 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9700_length_2102_cov_9.922325, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000317 DAWDXZ010000000
VERSION     DAWDXZ010000317.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9700_length_2102_cov_9.922325"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          515 a          694 c          511 g          382 t
ORIGIN      
        1 atgactgacg aaacgatgat tattgaaatt gcccgtaccc tcggcgttcg tcctcagcag
       61 gtgcaggctg ccctctccct tctcgacgaa ggaaatacga ttcccttcat cgcccgctat
      121 cgaaaagaag cgacgggaac actggatgaa gtccagctgc gctgtatcca ggaacagtac
      181 gcctatgaac aggctctggc cagccgcaaa gaaacggtac gccaatcgat tgaagaccag
      241 ggctgctgga atgccgaact ggaaaaggcc ctttccaaag cccgtcagct ccaagaagtc
      301 gaagacatct atcttcctta taaaccaaag aagcgtacca aggcatccat ggcctgtgac
      361 gccggcctcg aacccctggc caagctcttt gctgagcaaa atcccaaagg accgtctccg
      421 gaagaagcgg ccaaggcctt tcttaatgat gatgtcccga caatcgaaga cgccatccaa
      481 ggggcggcca atatcttagc cgaacggttc tccgaacggg ctgacttccg tcaaaaactg
      541 cgccgcagcc tctggcagga ggcccgctta acctgcagcc tccaagtcga agaaaaagac
      601 gccggcccca tgctgaccta cgccgatttt agcgaacgca tcacccgtct tccgagctac
      661 cgcatcttgg ccatcaaccg cggcgaaaat gaaaagaaac taaaggtcac gatcaaggaa
      721 ccgacagaca agcacatcga catgctgtgc cgattagtca tggagcgcca ttcgccgtat
      781 gaagccatca tcaagagtgc cgctgctgat agctataagc ggttaatctt cccgcaaatg
      841 gatcgggaaa tccgcaatga actgacggcc caggccgaaa agcaggccat cgacgtcttt
      901 gccgaaaatc tccgccacct cctcttgcag ccgccctttg aaggacaaat catcttgggc
      961 ctcgaccccg gatttcgcac gggctgcaag gccgctgtca tcagtgccac cggtcgggtc
     1021 ctcgactacg ggacctatta cctgacccat tcggaggcgc agaagaaaaa gagcgccaag
     1081 gatttggcca atatgatccg ccgccacggc gtcaccctca tttccatcgg caacgggacg
     1141 gcctcttatg aaacggaaca attcgtctcc cagctcatcg agaattacaa cctgagctgc
     1201 cgctacgtca tcaccaatga agccggcgct tcggtctact cggcatcgga attggctcgg
     1261 gaagaactgc ccgacttgga cgtcaccatc cgcggcgccg tctccatcgc ccgccgcatc
     1321 caggatccct tggccgaatc ggtaaaaatc gatccctctt cgatcggcgt tggccagtat
     1381 cagcacgacg tcaaccaaaa agatctggcg acggccctcg atcagaccgt aacctctgtc
     1441 gtcaactacg tcggcgtcga cctcaatacg gcctcagccg ccctcctcca gcacatcgcc
     1501 ggtctcacca cttcgacggc cggcaacatc gtcacctacc gcaacgaaaa cggtcccttt
     1561 aaaaaccgtc aggaacttct aaacgttccc cgcctgggac cggctacctt tacccaatgc
     1621 gccggcttcc ttcgcatcaa agacggcgaa gagcccctcg acaatacgtc ggtccatccc
     1681 gaatcgtatg acctggccgc tcaaattgcc ggtcagtacg gcctgacccg cgacgatttg
     1741 aaggaaccgg aaaaaatggc aggcctacgc gataagatcc agtgcaacgc cgccccgaaa
     1801 ctggcggcct ccctcggtgc cggcgagccg accatcaagg atattctgga agaactccgt
     1861 aagcccggcc gcgacgtccg cagcgagttc ccaaaacccc ttacccgcca gcacgtcatg
     1921 tccctcgccg atctcaaggt cggtaccgtc gttcgcggca cggtccagaa cgtcgtcgat
     1981 ttcggtgcct tcgtcgactt cggcatcaag acgccgggac tggtccaccg atcccagctc
     2041 agcaaccatc ccttccgtca tcccacggac atcgtccaca ccggcgacat cataaacgca
     2101 ga
//
LOCUS       DAWDXZ010000318         2098 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9713_length_2098_cov_9.141948, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000318 DAWDXZ010000000
VERSION     DAWDXZ010000318.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9713_length_2098_cov_9.141948"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          526 a          467 c          527 g          578 t
ORIGIN      
        1 cggaacttcg acaaattcag gaccacagag tttaaaagcg ttaatagcgc cgaggtaggt
       61 cgggctttct acgagaacga cgtcaccggg gttgaggaaa atctgagcca agagggacag
      121 accctgctgg gagccggacg tgatgacgat gttttcaggg gtgcagtcga ccatgaagga
      181 ttttttcatg cggcctgcga tctgttcgcg aaggggcgtg taaccttcgg tcgtgccgta
      241 ctgcagggct gcctggcctt ctttggtcaa tacagcttcg tcaacttttt tgagatcttc
      301 tgtcgggaac agttccggag ccggcaggcc gccagcgaag gaaataattt ccggcatggt
      361 cgtcagtttc aagagttcac ggatttcaga ggctttcaaa tgcatagcgc atatctatgt
      421 ttatatagta tcatttaaat tacttttatg caattaaacg tttatgtcat agctacattc
      481 tcatttatgc aaacctataa attcgtgggt attatggtca attactgata ataccaaaag
      541 taaactccat aaaatattaa gtaattcatg taaaaaattt ttaaaaaaaa taaaaaaaga
      601 aaagacgccc tcgggagaga gcgtctcatt tcctgcaaag tttaattatt ttcccatctt
      661 ttctttcaaa acttcagcta agcgtttaat gccttcgacg atgcggtctt ccggcatatt
      721 ggagtagttc aagcggaagt gattgccgtt gccgccgtta ggatagaagg ctccgcccgg
      781 gacataagcg actttctttt cgatgacttc cggcatgatg gccttggcat cgatgttttc
      841 gggaagggta acccaagtga acaggccgcc ttccggatag gtaaaggaaa ggccctgagg
      901 gaagtatttt ttcatcgaat cgtacatcaa ggtgcggcgt ttgccgtaga gttcgcggat
      961 cttggcgacg tgagcgtcga ggtcgtacat gtcgatgtag tacgaagcct ggcgctgagc
     1021 aaaggacgaa gactggaggt cgacggtctg tttcagcatg accgcttttt caaggatgtc
     1081 atgatgagcg accatccagc cgatacgaag gcccggcatg aagattttcg agaaggaacc
     1141 gaggaagatg acgttgccct tggtatccat cgatttcaag gtcggcatgg tctggccttc
     1201 atagcggagt tcgccgtacg gatcgtcttc gatgaccggg atatcatgtt tattcatgat
     1261 atccatgacg gctttgcgcc gttccagagg ccaggtgata cccgtcgggt tctggaattc
     1321 cgggatgacg tacatcaggc gtacgcggtc gccgtacttt tcaatagcct tttccaaggc
     1381 ttccggtacc atgcccttgt cgtccgtttc gacttcgacg aactggggga aattgacggc
     1441 aaaggcggtc gtagcgccca tgtaggtcgg gctttcaacg aggacgacgt cgccttcgtt
     1501 caaaaagagt tggccgagaa gagacaggcc ctgctgagag ccggcggtaa tgataatgtc
     1561 ttcataggtg cagtccgtat taaaggacga tttcatgcgg gcagcaatct gtttgcgcag
     1621 cggaatgtag ccttccgtcg tgctgtactg cacggcctga cgcccttctt tcttcaaaat
     1681 ttccacgtcc accttcatca tttcgtcaat cgggaataat tccggagccg gaagaccgcc
     1741 ggcaaaggaa ataatttccg gattttcagt gactttcagg atttcacgga tgtcagatgc
     1801 gtgcaaactt tttgcttctt cagaaaacat taccataaaa cttcactcct atctattgac
     1861 ttgctttcga tacgacgtat aacaacccgt cgtaatggtt tttattatac cacttcagag
     1921 tgtgttattc tacatttttt atgaaaaaaa acaggaaaag ttgctcgaat taagaaattc
     1981 gtaagcaaaa ccttttcctg ttaacggttt ttattcattt ttatcagctg caaaaaactg
     2041 ctgcgtcgcc ttttcgtctt ctttcatctg ggcgatgagg tcgtctgccg aggcaaag
//
LOCUS       DAWDXZ010000319         2093 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9732_length_2093_cov_4.626595, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000319 DAWDXZ010000000
VERSION     DAWDXZ010000319.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9732_length_2093_cov_4.626595"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    620..629
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1409..1418
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          429 a          558 c          586 g          500 t
ORIGIN      
        1 ggccgccttg tacggcgctt tgtcgtttat tttggatcct tttggccgcg ccgtcttggt
       61 cgtggccttt tggttctacc tgaccggggg actccatgcc gatggctata tggatacggc
      121 tgacggtctt ttttccggcc gcagccggga acggatgctg gacatcatga aggacagccg
      181 cgtcggttct ggcggcgtca tggccttcgt ctttttggtc cttttaaaag tgactttttt
      241 gagtcagctc gaaccgaaag ccgctgtctt cagcctcgtg gccattccgg cagcagctcg
      301 gttcggaacg ctgatcagta ttttccagtt tccctatgcc cgtaaagagg gactgggaaa
      361 ggccttcgtg acctatggtc cgccctatac gacggcgaag gccttgatgc tggctctctt
      421 gcccgtattc ctttgcgggc ctttttattt ggccctctta gggtcggcca tgatcttgtc
      481 cctatgggtg aaccacggca tcagccgaaa actcggcggc gtcacaggcg atacgtacgg
      541 cgccgtcatc gaaggcagtg aaatggtttt gcttcttctc gtcgttatcg gtagcaaggc
      601 cgttcatttc ctgttataan nnnnnnnnnc gcttattttc cggcgtccgt cctttttgta
      661 aggatgggac gacacgaagc tttttagatg aattttcgat aatggaggat attcatgatt
      721 acattatatt tagcgcgtca cggtgaaacg gacggaaatg tacagcagtg gtaccaaggt
      781 tctaccgatg tacccatcaa tgcccacggg ctggaacagg ccaaacacct cggtgacttt
      841 ttcagccata cccacctcga tgccgtatac agcagtaccc tccagcgggc catggccacg
      901 gcggaatgcg tagcggcgcc gcacggcttg tcggtcatcc cagatgaatc gttgaaggaa
      961 gccgattttg gtgaatggga aggccatacg tatgaagaga ttacgaccaa gtggccgggc
     1021 gagctggaag cggtctacgc gtcagacggg accttgccgg ctcggggcgg cgaatcgttc
     1081 tgccaggtcc gggatcgtat tttaaaggga acccgagaca tcctggccaa gcataaggac
     1141 ggcgacagcg tgttcatcgt ctcccacgga gcggccatcc gctgcctcct cttcggcctt
     1201 ttaggcctcg atatgcgccg tatctggtgc ttccaacagt tcaatacggc cttcaatgtc
     1261 gtcgaatatt acggcgaccg caacgtcttg accctcctca acagcacccg tcatttagaa
     1321 ggtacggccg gctatctgcc ccagtgggac aagatgcctt ctttatagga gcgcctatgg
     1381 gaaaggcgat gcgtctcgat aaactcctnn nnnnnnnnac tcctgggcca tacgggctgg
     1441 gggacgagaa aggaattaaa agacttatgt aagagcggtc aggtcacgat aaacggtgcc
     1501 atctgccgcg acagcagtcg taaggtcgat ccggaagcgg acgtcgtcgc cgtcggcgga
     1561 gagattgtat cttatgaaga atatatttat ctcatgctct ataagcctgc cggcgtcgtg
     1621 tcggctacgg aagacaacgt gtcgcagacg gtcttggatc tcctgccgcg ccggtatcag
     1681 ggaagccgcc tgtttcccgt cggccggttg gataaagata cgacgggcct cttgtttttg
     1741 accaatgacg gaacctgggc ccatggcatt acgtcgccga agaaacatca gcccaaacgc
     1801 tatgaagccg tcgtcgaagg cgatattccc tctgatatag gggaacgctt cgcttctgga
     1861 atcgtccttt ctgacggtct cgaatgcctg ccggcaactg cggaaaagac gggggaacac
     1921 accattaccg tcgtcgttga agagggaaaa ttccaccagg taaaacgcat gtgtgctgcc
     1981 gtcggactta cggtgacggc actccatcgc caatctatcg gtccggtctt tctcgacgaa
     2041 gggctgcgcc ccggtgagtt tcgtccgctc acggatgacg aacgagacag ctt
//
LOCUS       DAWDXZ010000320         2091 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9744_length_2091_cov_7.331532, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000320 DAWDXZ010000000
VERSION     DAWDXZ010000320.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9744_length_2091_cov_7.331532"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    468..477
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1279..1288
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          525 a          536 c          573 g          437 t
ORIGIN      
        1 gcggctatgg ttttggcggc caacagagtc tatgtcttcg gcttcggcaa ttcggccacg
       61 gtttgtgccg atatggagac ccggtttttg cgcctgggct tggccatccg ggccgaatcc
      121 gattcccata tgcaggttac cgaagcagcc ctgatgacgg ccgacgacgt cgtcattgcc
      181 gtctctcaca gtggggctac aaaggaactc ctccagtccg ttcgggcggc caagaaggcc
      241 ggtgccggca tcatcgtcat caccagccac ggccggtcgc tcctggcgga agaagccgat
      301 atctgcctct gcggcatggg ccgcgaagtc cagtacacgt cggaagccgg ggcctcgcgc
      361 ttgatccaca tggccattgg cgatgtcctc tacacgcgga ttgccatgca ggaaaagagc
      421 cttttccggg gcaatatgga aaagatgcga aaagaaatcg gcaagcgnnn nnnnnnntta
      481 cgcatggaaa agcagcctgc tgttcatgaa gcagcagact gcttttttcg ccaaggaaag
      541 gattatgaag ggaatctccc gttaggagtc cataaaaaag agccatgtcc accaatacac
      601 cgtaaatggt gtacatcagt ggcaaaccgt gacggtctgt ctgtcgttca caaacgacag
      661 aacatggttc ctagtcccat aagtatagcc gaattacgaa ctggcgtcaa taataccttc
      721 aaaagagtcc gagctgagat ccggcaatcg aaggcagcct tgataaggcg caaaaccgtc
      781 acatcatcta aaggcaaagg acagtccggc gtcaaaaatg gacaacaggg atcgtgcatg
      841 agtcgatgtt agccgcttcg attagaaaca taagtaaggc tatttcatga tatcataaaa
      901 aaagggccat gcccatccaa tacgccacaa gtgacgtaca taggatggca aaccgcgaag
      961 gcttgtctgc tgttcaacag cagaacatgg ttcctgatac aatcagtgta gcagaatcgt
     1021 tacgcggagt caatgatagg gtgaagagat ctaaaaggcg tcaactcaga tgtggacttt
     1081 ttgagcaatt tgtggaataa tagaatcgct atatagaaat gaggaaggat gatcagaggg
     1141 tgaaaaaatt tgtggaaatg gcgaccattg tcgaaaatac agccatcatt ccggaagttt
     1201 ggaaaatggt cttccaagcg ccgaagacgg ccaaggaagc caaggccggt caatttgtca
     1261 acgtccatcg cgatggggnn nnnnnnnncg tgggcctggc gccgctcatc ttcttggcga
     1321 aacagcttga tcatcccatc gtgctcgtcg ccggcaagac ggcagccgaa acgttttgga
     1381 cggagttctt tgaaccctat gccgaacaga tttatgtcac tactgatgac ggctctaagg
     1441 gaatcaaagg ctttgccgtc gatgccctgc cgaccatctt ttccgagcat cctatcgatc
     1501 gcgtatcggt ctgcggtccg acgatgatga tgaagaccat tgccgaagga tcggccaagg
     1561 ccggcatcga ctgcgaagtc tccatggaaa aacggatggc ctgcggcttc ggcgtctgtc
     1621 tgggctgtac ctttgaaagc aagctggacg gcaagcgcta taaagtctgc agcgacggac
     1681 ccgtattttc ggcaaaggag gtcttctgat gatcagtgaa cggattgccg tcgaatttgc
     1741 cggtatctcg atgaaaacgc ccgtcatggg ggcatcggga accttcggat tcggcatcga
     1801 atacagtgac tttctcgacc tcgacgccgt cggggccatc atttccaaag ggattacgcc
     1861 cttgccgcgg ccgggcaatc ccggtgtccg cgtcgccgaa acgccggcag gcatgctcaa
     1921 ctgcatcggt ttggaaaacc ccggtgtcga cgcctttaaa cgggacatcc tgccgaaggt
     1981 aacggcttcc tgtcaaacgc cgctcatcgt caatgtctcc gccggcagcg tcgaagaata
     2041 cggaaaaatc gcagccgacc tcgacgtcga cggcgtcgca ggccttgaag t
//
LOCUS       DAWDXZ010000321         2083 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9777_length_2083_cov_8.755424, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000321 DAWDXZ010000000
VERSION     DAWDXZ010000321.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9777_length_2083_cov_8.755424"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    785..794
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          430 a          578 c          581 g          484 t
ORIGIN      
        1 ccgggcgaaa acggcaaggc cggcaatgac gccgtcggct ttggccaaga gttccgcctg
       61 tccccggctg tcttcgggca aaatggcgtt ggtcgtcacg tcctcaccgg taatatcttc
      121 ctgcagggcc tgtaaaatca aaggatcagc ctgcaatttc atggttacgt tgttcatgac
      181 tctttttctc cctttccatt tcccctaaga tgctcttgcg atacacttcg gcaaggcccc
      241 cttcatggcg gctgacgtag tccgaagcct gagcgcaggc caggctgaaa tcgccgtgac
      301 tgtcgccctt ttcagccaga tccaaggccg cccgtttgcc gaaaaccagg ctttccaaga
      361 gggaattgct ggccaggcgg ttgcggccgt gaacgccgtt gcagctcgtt tcaccgacgg
      421 cgtagaggtg gtccatcgtc gtccgactgt ggccgtcgac gtggatgccg cccatgaagt
      481 agtgctgggc cggcgtgacc gggacccagt cgacggtggc atcgatgccg tcttccaggc
      541 agcgttcata aatattgggg aaatggccct ttatgacgtc cgttcccagt ccggtcatat
      601 ccaaccagac gaagggccgt tggtcctttt ccatctgttt gcggatagca gcggtcatca
      661 aatcgcgggg caggacttca ttggcaaagc gctttccgtc tttcccgcgc aatttagccc
      721 cttcgccgcg gaccgattcg gaaatgagga aactccggcc cggacgggac gaatagaggg
      781 acgtnnnnnn nnnncgacca tggtcgtgta gtcgcagata gtcacattgg gcagctcccg
      841 aacggcagcc aagaggcggc tggtgatttc cttgcctgta atatccttat gaaagaggat
      901 ccgcggccgg gagtgacagc cttcgcgggt atagtcgaaa gagccgtcgg cctttcgggc
      961 aaaatcgacg ccgtagccga cgagttcatc gatgatgcgc cgcgacgagc gaatcatgat
     1021 atccaccgat tcgcggcggt tttcgtaatg gccggctttc aacgtatctt ccatgaagct
     1081 gtcgtaatcg ttttcatcgt gaaggacgca gatccccccc tgagccagga aagaatcgct
     1141 gttttccacg tcatccttcg taatcatgag cactttctta tcgcgcggta cgtggagagc
     1201 ggcaaagaga ccgctggcac cggtgccgac gatgacaaca tctgtttttt ctgtttccat
     1261 gacatctacc ttcttctatt tggctaaatc cagcatccgt tccaaagcct gaacggcctt
     1321 ccctgccatc ttggcatcga caaagacttc gttcgtttcg tcgcgcaggc agtcacggac
     1381 cttttccaag gtgaccttct tcatattgat ggataaaaga ccttatcggg attccgcgtt
     1441 tccagctcat aaaagacgcc gccttcggtt ccgatgatga attccttggc atccgacgaa
     1501 ccggcatagt cgatgatgcc tgacgtgctg ccgacgtaat cggccagggc caggacttcc
     1561 ttgcggcatt cgggatgggc caggaacagg gcctgaggat gagcggcctg taccttcttt
     1621 acgtagtccg gatcgaccgt atcgtgacgg ggacaaaagc cctgattgag gataaaatgc
     1681 ttatccggca cctgggtcga aacgtagtgt cccaggtttt tatccggcgt aaagaaaata
     1741 ttcttctggg gcaggacgac gtgacgcaga cgtcggacgc agcttttacg gctgccgtcg
     1801 aattgacgta acagacgacg gccaagtcgt catactcggc gcgggccttt tcgatcgtcg
     1861 ccgtatcggc catgtgggcc atgggacagg acgccgtccg atcgggcatg aggactttct
     1921 ttttgggatt gagcatcttg gcgctctcac ccataaaatc gacgccggca aagacgatga
     1981 ttttagcatc cgtttcaacg gcctttttgg ccagaccaaa ggagtcgccg acgtagtcag
     2041 cataggcctg tacctcatcg ggcacgtaat agtgggcgag gat
//
LOCUS       DAWDXZ010000322         2081 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9787_length_2081_cov_9.121915, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000322 DAWDXZ010000000
VERSION     DAWDXZ010000322.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9787_length_2081_cov_9.121915"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          431 a          643 c          480 g          527 t
ORIGIN      
        1 taccgttatc ttcacggcat ccaacgtata cttgggctta aaagtcggtc tgacgttctc
       61 ctcgtccatt ccggctgcag tcatctccat ggcaatcctg cgcatgttca aagattccaa
      121 cattttggaa aacaacatgg tacagacgca ggcatcagct gccggcacct tatctgcaat
      181 cattttcatt ctgccgggct tgctcatgtt gggatactgg caaggattcc ccttcatgga
      241 aacgttactg ctctgcgcct gcggcggttc tctcggggtc ttatttacga ttcccctccg
      301 ccgtgccatg gtcgttaaca gcgacctgcc ttatccggaa ggccgagctg ctgctgaaat
      361 cttaaaagtc ggcagtgaaa tccgcaaaga agaagcggac ggagaaacgg gaactaaaaa
      421 ggaaacgggc atgaaagaca tcgtcggcgg tactgccctg gccggcctgt tcagcctttg
      481 ctccaacggc ttccacgtcc tctcctcgga attcagctac tggctccact tcggcaaagc
      541 cgtcacccag attccgctcg gtttttcaac ggcccttctc ggcgccggct acctcatcgg
      601 catcaccagc ggtatcgcga tcttagtcgg taccctcttg tcctggtacg tcttcgttcc
      661 ctatctgacc tcgatgctga caccggctga aggccaatcg gcagctgctt ttgcccagac
      721 aatttgggct cagaaagtcc gcttcatcgg tgccggttgt atcggtatcg ccgctgtctg
      781 gaccttaatc cgcttggcaa aaccggtcgt cgacggcatt aaaatgtcga ttgacgccct
      841 tcgcgccaac gacacagaag aaaagaaaca gctccaccac acagatatcg atatgactcc
      901 gaaatcggtc ggcctcgtat tcctggctat ccttatcggg ctgttcatca ccttctataa
      961 cttcgtaagc aacgccggtc tgtccttatc agtcaccctc atgttcatcg tcatcggcat
     1021 cttagtcgcc atcctcatgg gcttcttcgt cgccgctgcc tgcgggtaca tggccggttt
     1081 gatcggcaca tccgccagcc cgatctccgg tatcggcatc ctcggcatca tcatctcgtc
     1141 cttggtcgtc ctcggcctcg gctcttcagc cggtgtcttt gaaacccttc agggaactca
     1201 gtttgcaacg gcattggcca tcttcatcac cagtgttatc gtcagcatcg ctgcgatctc
     1261 caacgataac ctgcaggacc tcaagaccgg ctacatcgtc ggtgctacac cgtggaaaca
     1321 gcaggtcgct ctcatcttgg gctctatcgt cggcgctatc gccatcgctc cggtactgaa
     1381 cctcttgtac caggcttacg gctttaccgg tgcactgccc cgtgccggca tggatccgac
     1441 tcaggccctg gcagctccgc aagcaacctt gatgacgacc attgcccaag gtatcttcag
     1501 cgccagcctc gactggaatt acattctctt cggcgtcggc gtcggcatcg ttgccatcat
     1561 catcgattta ctgttaacga aaaacacgaa agccctggcc ttaccgcccc tggcaatcgg
     1621 tatgggcatt tacttaccgc ctacccttga aattcctttg gtcatcggct ccgttatggg
     1681 ctacttcgtc aatcgttcct tgaaagctcg tgccgctcgc cgcagcccgg gccatgaaga
     1741 agaagatgtt gaagcctgca accaccgcgg cgtcctcttt gcttccggtc tcatcgtcgg
     1801 cgaaagcctc atgggcgtca tcatcgccct gctcatcgtc atctctgtca cctccggcgg
     1861 cagcgaaaac cccttggctt tggttggcaa agacttccag tcgacagccg atattctcgg
     1921 gctcatcgcc ttcattgcca tgatcgtcat cttcatccgt cacatcttca tcaccaagtt
     1981 cacgccggaa aacggttcga acaagtaagt aagatatacg gtgtattaaa tttctgttag
     2041 aaattaaaaa agtttttcta aaaaaatcct cgcttcgatc a
//
LOCUS       DAWDXZ010000323         2074 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9821_length_2074_cov_5.747400, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000323 DAWDXZ010000000
VERSION     DAWDXZ010000323.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9821_length_2074_cov_5.747400"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    626..635
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1374..1383
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          534 a          485 c          555 g          480 t
ORIGIN      
        1 gggtagtcgc tgctcataaa ggacgtatag ctgccgaagg caaaggccag ggtcaaaaag
       61 agacagataa taacgcgagc catttttaag acctccttcc gcgggctgcc gcatggcagg
      121 cctggccggc ataactggcc gttaagctgg caaagagaac ggtgagtacg accaggacac
      181 gcgtcatgat agaaatcgta ggcaagggca aaagatcact ataggcatag gttggaatga
      241 aggagatggc gatggacgcc aaaatgacca gggtgagccc ggcatataag aagccgctta
      301 gaatgccgaa caaagtgccg agaatgtcta aaaaaggact ggcaacggcc ttggctgtat
      361 tgtttgcccc gtcattccaa ttgaaatcac gccccgtttc ccggtccggt tcttggtacg
      421 atcggcgttg gggcggacgg gactggtagg accggcgagt cttccggggc cggccatagt
      481 cgtccggctg ttccggcaga tcatagcggg aagaggcagt ccccgggccg aaatcgtcgt
      541 attcttggta cgagtcgtca tagtcatctc cgggcatgcg aggattgtct tcggggatga
      601 tgggttcgcc gatatagtaa cggtannnnn nnnnntggtg aaatgatgcg ccgtattgcc
      661 agacggcaat ggtgaccgcc gccgtgacgg cgatgaggat gaagacaccg taggttggca
      721 tatcgggtaa aaaggcaatc gatgccagcg gttctttttc taaggcgata cagacagcta
      781 tccagcccaa aagggcgacc atgcacaagg tcaggacaga gccgacagca taacagagtc
      841 gatataatcg gcgcaggctt tgctggttcg tcgatgacgt caaaaaaatc cctcctcgtt
      901 tctgtgatta tcccttatct taacacagcc tatgaggtcg gtcaattgca cccccttttc
      961 ggacagcagt gacttgcagg gccatggttc agactatata atgatatata tgcaatcatt
     1021 agcttctgat agaggaggaa ttttttatgg ctgattttgt ccgttactat gtaggcgacg
     1081 tggtccaaat gaagaaggcc cacccctgcg gctgcaatga gtgggaagta cttcgtatcg
     1141 gcgtcgattt tgtcatccgc tgcaccggct gcgggcacag ggttatgatc cccagaccga
     1201 agtttgaaaa ggctgtcaag aaacggctcc attctcatgg cgaagattcg gaaaaagcgt
     1261 aaaaatttag aaaaaggtat ttttattttt ggaactttgt ggtacaataa tcaacagtta
     1321 atattttaat actttaccaa gataaagcga taaagaaagg gtggtcatag gatnnnnnnn
     1381 nnngaacaat aataccttgg atggagtgaa gatgctccac catgatgaaa tgcgcaatta
     1441 tgaccgaatc cagggaaaaa tcatttccgt catcatgaac cacggctgta agattattga
     1501 gacgcccagt tttgaagatt acgatgtgta ccggcacttc tttcctcatc ttcgccagca
     1561 aatggttaag accatcgata ccgacggtcg ggtcctcgtc ctgcgccccg atgtgacgct
     1621 gcccgtcgtt gaaacggcgg cccgagaatt tcctcgtgcc aaccagctca ttaaattcgg
     1681 ttatgtcagc actgtctttc gtgaatacta cggccggtcg acatatggca aggacttttt
     1741 acaaggcggc attgaaattt tgggcgactc cagtccggaa tgcgacggcg aagtcatcgt
     1801 tacggcagca gagatattaa aggctgtcgg cgtcgaaaac attcgcatcg atatcggtac
     1861 ggctgcctat acgaaggccc tgttcgaagg tcttcccatt tcggcagaac agaaggaaac
     1921 cctgaaacga tacatggcag aacgaaacct cgttgcctgt aagtcctata ttgagtccct
     1981 ctccctttcg gcggaagccc gggaagccct catttccctg ccggttctgt tcggttccta
     2041 tgcccagact ctcagcaagg ctcgagacta ttcg
//
LOCUS       DAWDXZ010000324         2072 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9830_length_2072_cov_9.874070, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000324 DAWDXZ010000000
VERSION     DAWDXZ010000324.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9830_length_2072_cov_9.874070"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    738..747
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1412..1421
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          466 a          599 c          565 g          422 t
ORIGIN      
        1 gaccgatacc ggtcgtcgtg atgaagaggt ggtcgacctg gcccttaccg gcaaccttag
       61 tgtccccggc aacgatgcgg acgccggctt cagcagccgt ttcagccata gccttggcgt
      121 aggcttcgac tttttccatg ggaaagcctt cttcaatgac gaaggcacac gataagtaaa
      181 gaggtttcgc ccccatgcag gacaagtcgt tgaccgtccc gcagatactg agtttaccga
      241 tgttgccgcc gggaaattca ggcggcgaca cgatgaagcc gtccgtcgta aaagccagat
      301 tcgttccgcc aataggcaaa acagcggcat cgtccccggt aaactgggga tttgaaaaat
      361 gagctttaaa gacgcggtca atgagttccg ccgtctgctt gccgccggca ccgtgtgcta
      421 aggtaataag gtcactcatg ggcatttcct ccatataaat aataggccga gcaggcacct
      481 tcgtgagaca ccatgcaggc gccgaccggg tgttccggcg tacataattt tccaaataag
      541 gggcattccg gcggcttgca ctggcccttc aggatgtcgc cgcagcggca gcccggattg
      601 gctttgccgt ccatctttgg aatgccgtac ttcttgcggg cgtcgcaggc agaaaatttc
      661 tcccgcagct gcaagcccga ctgggggatg acgcctaagc cgcgccattc cgaatcgcag
      721 ggttccatat attcagcnnn nnnnnnntat tcagccatca aggcctgggc cgagcggctc
      781 ccctcttctg taacgacgcg gggatagcag tttttaaaga aaggcttccc ctgcgggaac
      841 ttggccaaga tgacggcaaa ggccgtgagc agttctttgg ccgtaaaacc ggcgacgaca
      901 ccggacatcc cttccttcac catggcttcg cctgccttag tgcccacgac ggcatcgacg
      961 tgaccgggat agaggaaggc atcggcactg cccttcatgg cttcataggc catgggcatg
     1021 gtcttattgg ccgtcagcag ggagaaattg gtcaagccgt cagccatagc ctgcttaacg
     1081 gccaggcacg acggaggtgt cgttgtttca aagccgacag ctaagaacac gacttcttcg
     1141 tcacgatgag ccttggcgta ttcataggca tcaatgggcg agtaaacaat ttcaacgtga
     1201 gcgcctttac tgcgggcgtc ggccaagctc atcgaggtgc cgggaacgcg gactaagtca
     1261 ccaaaggtgc agaccgtaac ccctttttcg agagccagcc acagggcctg atcgatatag
     1321 ccgacgggcg taactcagac cgggcagacc ggtccggaaa tgacgtcgac ctgcttaggc
     1381 aaaatctgcc ggatgccgat gcggaagatt tnnnnnnnnn ncggcgtgga ccatgacgta
     1441 atcgccggga tccaattcat cgatcaaagc ggccgatact tcccgtttcg ctccggacgc
     1501 atcgacgagc gccattccat tttgaatttt cataacacgt gcgggtaaac ctacacacat
     1561 gataacccct ccttcatcta tctaaacatt ttcatggtac atcgcatata tcgcctgtcc
     1621 caaggcaatg ccgccgtcat tgggcggcac caggctgtgc cgtaagaccg taaagccgcg
     1681 ctgctgcagg gcctcatcac aaaggcgcaa aagcagtcga ttttggaaaa caccgccgct
     1741 tagggcaacc gtctcaaggc ccgtctgact gcgaactttt gcgcagatgc cgacagccat
     1801 accggccaat cggcagtgaa attcataggc caatttatcg ggatcgtcgc cggaaaggcg
     1861 ttgttcgacg aggtttttga ccagcgccga cgtcggcagc agccatcgtc cgtcgccttc
     1921 aacgggctca ataggtacga cttccatcgc cccgtgagcc ttttcccagc gttcagcagc
     1981 aaactgcagc gtcatggccg cctggccttc aaaggtcgac gaccggcaca ggcctaagat
     2041 ggcgctgacg ccgtcaaaga ggcggccggc ac
//
LOCUS       DAWDXZ010000325         2066 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9844_length_2066_cov_10.889110, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000325 DAWDXZ010000000
VERSION     DAWDXZ010000325.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9844_length_2066_cov_10.889110"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          507 a          603 c          563 g          393 t
ORIGIN      
        1 tactgtacat ttaaacaacg ggctgtcatg cagcaagaaa aggaaacgaa gaaaggcatc
       61 tttttatccc gccggtacta tcgggcggca ttgttgtcgt tatttacttt ttttgaaggc
      121 gttatcaaca actggatcaa gaccatcatc caggaccgac cggaattttc cggcgcagcg
      181 gatcagcaga cgctgaaaaa atgcgacgcc gtcatcgaat actgtttctt ctgttcgtat
      241 accaagcata ccggtacctt tacctcgctt tacggctaca tcaaccgcta tgaacagcac
      301 gatttggccc tcattgaaca catcgacgga cagaccctcg acgccatcga aacggcgatg
      361 gatgagtatt tctgttacgt cgaagccctg accggtctca agcgcctgcc caagcccaac
      421 caatcgacga cgggcctggt cggacgcatc ggcggcctgg tcaaggactg ccacggctga
      481 taagaaagga ttgtggtctc aatgagtgat aagtattata tcggattcga cgccggtaca
      541 cagagcgtca aagtagccgt ttacgacgat gaacagcact gcctggccat ggatacggcc
      601 aagacccatc tggcatatcc taagcccggc tgggtccaga tggatgtcga tgaatattat
      661 cgcctcatca aacagtgcat ggcctcgtgc agccgccaga tggaagaaaa aggctgggac
      721 gtcagcaaag tccgggccat catgggcgac ggcatcatct gcggcatcgc cggcatcgac
      781 gaagacggca aggccattac gccctacgtc aactacctcg acagccgcac ccgcgacgac
      841 gtcgaagccc tgaaagcaaa gaagctcgac atttggggac gggaaacggg caacgccgaa
      901 ccgaacatga tgttcccggc catgttcgcc cgctggttca tgaagaacat cccggcctgg
      961 aaggaaaagg gcgtcaaatt cgtccacaac tgcccctacg tcttaatgca cctggcaggc
     1021 cttacggcca aggacgcctt catcgactgg ggcaccatgt ccggctgggg cctcggttac
     1081 gacgtcgtca aaaaagaatg gtccaaggaa caactcgaca tcctgggcat tccagaatcg
     1141 gccatgccgc gcatcgtaaa gccttgggac atcatcggtc atctctgtga agaagacgct
     1201 atggaaacgg gctttcccgc aggcattccc atctgtgccg gtgccggcga tacgatggaa
     1261 tccatgatgg gcagcggcat cttggaagcg ggcaacgccg tcgacgtcgc cgggacctgc
     1321 gccatgttct gcgccgcaac ggacggcatc atgccggaac tgtcggtccc cggaagcaac
     1381 ctcatcttta actccgggac cctgccggga acgtacttct actggggcat ggtccgcacg
     1441 gggggactgg cccttcgctg gttcaaagac agcatcgccc gagaagaagg ggacgactac
     1501 tacagtaccc tcgacgaact ggccaagcgc cataaaccgg gctgcgaagg ggccatgttc
     1561 ctgccgtacc ttacgggcgg cgacagcgct taccccaacg tccgcgggac ctttacgggc
     1621 cttaccctcg attccgacca gggagccatg tggcgctgcg tcctcgaagg catcggctat
     1681 gactatatgg aagtcaccga ccaataccgg gcagccggca tcggattgaa gcacctgacc
     1741 attaccgaag gcggcagccg ggacgaactg tggaaccaga tcaaggctga catgctcgat
     1801 acgacggtcg tcaccttgaa agtcgccggc ggagccgtac ctaccaactg catcgtcgca
     1861 gcctacgcgg ccggcgatat caaggacctg aaaaaagccc ttgaacgcca gttggaagtc
     1921 aaggccacct acgttcccga cgaagaaaat accaaaatgt atcgcgcttc ctatgaaaaa
     1981 cggggagccc tcttaaaagc cctcaatggg ggagactaag agcctcacgt gccgtacagg
     2041 ttttgcctgc tgcggcactt tttttt
//
LOCUS       DAWDXZ010000326         2063 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9857_length_2063_cov_7.429781, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000326 DAWDXZ010000000
VERSION     DAWDXZ010000326.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9857_length_2063_cov_7.429781"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1629..1688
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          518 a          508 c          510 g          467 t
ORIGIN      
        1 gtaaaggtcc gtaagctgtg aagggaaaac cctttgtaat tagtatacca taattttaca
       61 tctttttcga agttttttcg tatgtaaaac tacctatacg aaagccgccg tttccagtat
      121 actagaagaa aaaaacggaa atgagggata tatatgcgaa tcttgcagga aaccttacgg
      181 gccgccattg cccaagcctc gccggtccta ttcgacaaaa aggcgacgct ggaaaaagtc
      241 gtatcacaaa tcgaagaagc cggacggaac ggggcacagc tcatcgtctt tcccgaatcc
      301 ctggtgccgt gctaccccta cggcctgacc ttcggcttta ccgtcggcag tcgttctgaa
      361 gccgagcggg ccgattggaa gcgctactat gaagaatctg tcgtcgtcag ccgtgatctg
      421 gagtccgttg ccgctgccgc taaggccttt ggcatctacg tcagcttagg catcaccgag
      481 cgcgatgaaa cgacgtattc gctctactgc agcaacgtca tcttcgggcc ggacggcacc
      541 ttgaaagccc tccatcggaa aatcaagccg accggtgccg aacgctatat ctgggccgat
      601 tctcacatcc cgtcgacgta ttttcctatc gtccagacgc cgtggggccc catgggatcc
      661 ctcatttgct gggaaaatta catgcccctg gcccggacgg ccctctatca aaagggcgtc
      721 agcctgtatt tggcgccgaa taccaacgac aatcccgaat ggcaggatac gattaagcac
      781 atcgccatcg aaggtcactg ctacgtcttt aacgtcgatc agtactttac caaggacatg
      841 tatccctccg acctggtgga aaagggcgcc gtcgataaac tgaatgacgc gacctgccgc
      901 ggcggcagct gcatcatcga tccctgcggt cattatctca ccgagcctgt ttgggacaag
      961 gaagccctca tctatgccga tctcgacatg aatcaagtcg ctctgcgcca catggaattt
     1021 gatgctgccg gccattacag ccgtcccgat attctcgaac tcatcgtcca cgaataagaa
     1081 aaatcgggga acataagcgt ttttagacat cataaaacgc cgttaaacga agtttatcct
     1141 cgtttaacgg cattttctat aagcgcccgt aaccggcagt aagggaggcg gggcatgccg
     1201 gtgagggctg ggggattcag gcaattttat tgccgttggc aaccatttta aattgaaggc
     1261 ctaagacttc ggcagcccgc ttgcacatga gcatgcgctg catgaagatt tcaaaataat
     1321 ccatgaggga gcatatcgat tcatcgagct cgatttccaa gcggatgacg ttcttgtggc
     1381 ggtcgatgtg gagtttggaa gaaagagcgg cataattgac ccggtcgtgt ttatcgaagg
     1441 tttcgggaac cttattgcgt acgcggttgc ggcgaacatc gctcttgtct gccaggatga
     1501 cagctgctga aatggggtca aaggcgacgc cgcttcgttc atcgtgctga ccgatggccg
     1561 agatgatggc ggcaatgtct tccggcggag cgccccagtc gcgaaggaac tgaaaggcca
     1621 tgagggctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnngt atatattgcg taaaaatccg ataaaaagta cgctttattt tgaaaaaaag
     1741 tttatttatc gattttcttt gtgaagcacc gtcagtaagc gtataataaa ggtatatttg
     1801 aaagggggat tatatatgaa cgaagttatg aaagcactct tagaacggcg cagcatccgt
     1861 cagtacgaag acaaagccgt tcctgccgaa ctcattgaac agatcgtgga agcaggtctc
     1921 catgctccgt ccagcatggg caagcagaat tcaaaaatcg tagtcatcac caataaggaa
     1981 ctgatccacg aattatccgt cgtcaacaac gaaatcggcg gttggcctga aggaaacgat
     2041 cccttctacg gcgctcccgt cgt
//
LOCUS       DAWDXZ010000327         2057 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9887_length_2057_cov_8.947053, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000327 DAWDXZ010000000
VERSION     DAWDXZ010000327.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9887_length_2057_cov_8.947053"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1255..1264
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          563 a          470 c          498 g          516 t
ORIGIN      
        1 ggcaacatcc attatgtcca agaaggggac aatctggttc ctgccggtga tacggaattt
       61 gctaaggata agacctttgg ttatacggct tctgaaatgg gtgcctatat tgaagaaaaa
      121 acgaaaggct tatataaggc ttccgatacg acgtatattt ccttagatga tttacgaaat
      181 ctccgcgtcg atgcaatcga aaaacagttg atggaagtaa aagacttcaa caaagtcatc
      241 gtcaacgctg tcgattacga agatgtacaa gtctttgcca tcgccatgat tcgggccctg
      301 aaagccggta aaaacttcct attccgtacg gcagctgcct ggcctaaagt tatcggcaat
      361 gttcccgata aagcccttct ccaaaaagaa gaacttattg taaaaggaaa taccaatggc
      421 gggttaatca ttatcggctc ccatgtcaag aagacgacgg atcaattgaa tgaattaaag
      481 acctgcgaac aggtaaaatt catcgaattc aatcacctct tggtattgga accggaaaaa
      541 ctggaatccg aattacagcg catcgtaaag acgacagaag aagccatcaa gcaaggtacg
      601 accgttgccg tctatacggg gcggaaacgc ttcgatgccg ggtcggaaga agaatcctta
      661 cggatttctg ttaaaatttc cgaagccatt accagcattg tccatcgcct ctccgttcag
      721 ccggctttct tgatcgccaa aggcggcatc acctccagtg acgtcggtac caaaggcctt
      781 agtgtaaaaa aagccctggt tttgggacag gtttcgccgg ggattcccgt ttggaaaaca
      841 ggggcagaag ccaaattccc caatatggcg tacattatct tcccggggaa cgtcggtgcc
      901 gtaacgacgc tgcgggacgt cgtatccatg ttaagtaaga gataggtgac gcacgatgct
      961 gattaactta cgaacgatgt tgaaagcggc ctcggcaaag aaaatggctg tcggagcctt
     1021 caatgtttac aatgtagaat cgttacaagc cgtattggca gcgaccaaag cctcaaatca
     1081 tgccgttatc ctttcttttg gcgaaaaata cgaccaacat atgccgatag aagccatggc
     1141 tgaattagtg aagttttatg gtcatacctc aagtcaaccc atcgtgcttc atttagatca
     1201 ctgcaaacag gaaagcaatg tcattcgtgc tattcgggca ggatttacgt ctatnnnnnn
     1261 nnnnctatca tgtatgacgg atcgcatctt cctgtcgccg aaaatgcatc ccgaagcgcc
     1321 gaaattgttc ggatttccca cgccgtccat gtcagcgttg aaggagagct ggggtactta
     1381 aatgaggaaa cgggacaggg gccggtcgga aatgaagata cccatactca agtccgggat
     1441 gtggcagctt atactgccgc atccggagtt gatgccgtcg ccatcgccat cggcaacgct
     1501 catggcatct ataagggcac gccgaagctc gacttccagc ggctggcaga cattgcacag
     1561 gccgtcacga cgcctttggt actgcatggc agttccggta ttcctaaaga agacttgcaa
     1621 aaagctattc gacttggcat tcgcaaagtt aatatcaaca cggaagtatc gacagccggt
     1681 gtgaaggccg ccagagcctt cttggctgaa catatgaatg aaaacacacg ttttgaagag
     1741 gtaaccaaag cggccgaaca aacaatgacc aaagtagtag cctcttattt accttggctt
     1801 gatttatctg aatagggggg acgtattgtg gaccttattt tcttagtcgg aaccttgttg
     1861 gctgccatta tattggtcgt cgttttaatc atgaggcttc atttacatgc ctttgtcagc
     1921 ctcgtcatcg cttgcttctt cgtcggcttt gtaaccggta tgccgctgct gcaaatttgt
     1981 aaatctattg aggccggcat gggaagtacg ctcggctttt tagctactgt attgggcttt
     2041 ggcagtattt tagggaa
//
LOCUS       DAWDXZ010000328         2057 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9885_length_2057_cov_11.265235, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000328 DAWDXZ010000000
VERSION     DAWDXZ010000328.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9885_length_2057_cov_11.265235"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    557..566
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          535 a          433 c          492 g          587 t
ORIGIN      
        1 aaaaccgacc tcctaaagtt agatttttag gtccaacttt agggggtcgg ttcagtccac
       61 ccctctcttt caaaaacttt acaaaaaagc ccatttttcc aatttgattc tttcgttctt
      121 cccagtattt catctccttt tatcaatttc actatttata cattccgagt gataattttt
      181 acatccaaaa acctaaagtt ttctgaactt tcaaaaaatc gtaaactaat acatctgtct
      241 ttcacagaaa tacaaaaaaa gtcccttcca ggaaaaaatc actttcccaa aaggaacttc
      301 acaaaagaca aaaagagtct aaaatcacaa aaaaatgatc tcatctatat ggatttcgac
      361 tcataaaatc caatctacaa aataaaaatc ctacccggca taattttatc tgccctcatt
      421 tatctcatca tcttttgtct ctaattattt tatcttaatc ttgtcttatg tatattgtct
      481 tatctcttct tattcttatc tcttatcttt ctcttcttat acgtaaagcg ttacatcaca
      541 gattgcctcc ttttcannnn nnnnnncagc gggagggcat gacgtgtaat gtctttcagc
      601 gtcgaggtac agaccggggc attcttagga tgccgatgag ctgtgtataa gatggggaag
      661 gcctttccgg tattgtcgtc cttatcgtgg gttgcggtgt atgaaagggt atagcggtca
      721 gccggttttg ataaggaaca gtcctgtgaa accgtaaata gcctttctct tgtgctgaca
      781 gcgaggtatt gatcgtggcc cttcgctgcg tctgcagatg cttttaaggc tgtgttacaa
      841 gagggagcag cctccatttc ctccgggact tttgaaaggg ctgtctgtct tagaaccggg
      901 aggtggtact gccgtctttt caaggggcgc agagacaaag aatcggggtt cacgaagatg
      961 tccgtgaacc ccgattcttt gttttagccc gtaaccctta ttctcggccc gtaagcagtc
     1021 ctgataaaat gttgacgtac aggagcgctt ccggcgaaag agcttcctct tttatcttta
     1081 tccagccgac agtcatcgta tccaagggat tggcaatggg aatggccgtg atgtggcgtc
     1141 cgatgatggc cgatgcgaca tgccccgttc cgatggtata ggcgtcggtt gcgcggatga
     1201 tgctgaacag tgtcgatcgg tcggtgacat agatgctctg ccgattggat gggtccgata
     1261 ggagcagttc ttcagaaaac tgcgagcggt cgtgcccttg ttcgaagcgg acgcagggat
     1321 agtccttaag cttgccggga gggatgcttt gactctctgc cagggggtgg tccaagcgga
     1381 cgtagataca gggcgtaaac gtacagagag acgtgaattc cagcttgttc ttttggaaca
     1441 ggtcctgcaa aaagttttcg ttcatgtggg ataaaaagag gatgcccagt tggctctcac
     1501 gggtgacgac gtcgtggatg acctgtgatg tccgaccttc acgcagggta aaggtgtagg
     1561 ctgatgtcgg cgatatgcgc tgcagagcat ggatgaaagc ctcgtcggca aaggggtagt
     1621 gctgagatga aacggtcagg gtgtgcgagt cttcctggcc ggtttcaggg tgaaaatgat
     1681 catgcatttc gtcagcatga gctaagatgt ggtcggcata gtgcagaaac tctaacccgg
     1741 ccgatgtgaa cgtgatgccg tgacgattcc gcttgaccag cgtcagggaa aattctgttt
     1801 ccaagtcttt gatggcggca ctgagactgg gctgggcgat gttcagagct tgggcggcgg
     1861 cagaaataga attatgtttt gataactcaa ggatataacg caattgttgt aaagtcatag
     1921 taaaaccacc tcaaatataa accgtaccta tagagacgcc cttcttagct ttattgtatc
     1981 aaaataagca aagttgggaa agttatgatt ttttgacaca gtaaagaaag ttttaaaatg
     2041 aaaattttaa tctcgaa
//
LOCUS       DAWDXZ010000329         2050 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9924_length_2050_cov_5.956391, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000329 DAWDXZ010000000
VERSION     DAWDXZ010000329.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9924_length_2050_cov_5.956391"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    937..946
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          492 a          564 c          497 g          487 t
ORIGIN      
        1 ctgacgtaca tccgtatctt catcgacgac gaccgacgcc agctgccaaa cggtctgccc
       61 cggttcaacg cgaacggaaa cgtattcctg tgaaggctga tagctccagc cgatttgggt
      121 gcacataaag atagccaggc caatcaaggc gacatatttt agtaacgggc ctctcccttt
      181 tcgtcttctt ctcatggtta tcactcctaa gtaaaaacga acgttctaag aacaagtgtt
      241 cgtcgacacc cttattatag cttcgaacag ttgttcttgt caaggcgatt tgaacaaatg
      301 tttgattttt ttcggtattt tcggtataat gtgactagct atactggaat gattgtaact
      361 tgccagagac tggagtgact atatatgatt accaatgaaa aaatgctgac gacccgtcaa
      421 aaacagatat tagaatacat ccgcacctgt gtccaccaat tcggctatcc cccgtcggtc
      481 cgggaaatct gtaaagaagt cggcctcagt tcgacgtcga cagtccatgg ctatctccat
      541 cgccttgaag acctgggcta tatccgccgc gacccggcta agaaccgaac gatcgaactc
      601 ctcgacgaag gttcgtggcg ttcaaagaag atcgttcccc tgcccctcgt cggccaggtc
      661 cgtgccggag ctcctatctt tgccgaagag tgcgtcgaag acgtctaccc cttcccggct
      721 gatttcatcg gcaatgacga ctgcttcatg ctcatcgtcc aaggggacag catgaaaaat
      781 gccggcatcc tcgaaggcga tcagctcatc gtccgccatc aggatacggc agaaaacggt
      841 gaaatcgtcg tggccctcat cgacgatgaa gcgaccgtaa agcgttattt ccgcgaaaaa
      901 gatgccattc gactccagcc ggaaaatgat gaatacnnnn nnnnnntgcc ctctttgacg
      961 gcatccgccc cgaagaccgg tcagccatgc tcacctgcct cggtgcccag acgaagaccg
     1021 tccggaaagg ggaattcttg gttctggccc aagacgaagt ccgctatatc ggcgtcgtct
     1081 taagcggtga aatccacatg atccacgaag accgctgggg cgacaaggcc gtcttggccg
     1141 tcatcaaaga aggcggcctc tttggggaaa ccttcgtctg cgctacaatt ttgcagtctt
     1201 tagtcacctt ccaagccgtc aagacgacga ccttcctgac cttgcccttt caaaaagtcc
     1261 tccacgtctg tacacaggcc tgtccttttc atcatcggct catcgaaaat atgctgcacc
     1321 tcattgccgc aaagaacgcc cagctcatgg aaaagttgga gatcgtctcg aaaaaaacct
     1381 tgcgcaaaaa gctgctgacg tatttttcct tccaatcaga aaaggccggc agcccgacct
     1441 tcaccattcc cgtgacgcga agccagctgg ccgactacct ctgtgccgac cgaacagccg
     1501 tcgcccggga aatgtcgcgc atgaaagaag aaggactcat cgatgtaaat caacagaccg
     1561 ttaccttaaa ctaaatacaa aaaagggagc actgccaagg catttggcgg tactcccttt
     1621 ttgcatgccg tatattacag atttacttct ttaaagccag gccgtcgtgg aaggctttgc
     1681 cgacgacggg gccgatgagg cgatacgtct tggtgctgct gtcatacatg atgggctgga
     1741 gtttgtcgta gtcgacgagc ccctcggtga ggaccgattc atcagcttgc tgagctacga
     1801 tttttccgac gagaatcccc gtttcgtcgt catagctctt gcattcgcat tcgagggtga
     1861 cgggaaattc ttcgatgatc ggggccttga cgtggcggct ttcatggacg tggcagccgg
     1921 ccacttcgat cttattgacg cggttccccg tttcgacgcc aaaataatcg gccaggacca
     1981 tcgtatcctt ggtcgcaaag gcaacggtaa aggcgccggt ctttttgacg ttgtccgtcg
     2041 tcttgtgttt
//
LOCUS       DAWDXZ010000330         2048 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9929_length_2048_cov_11.873056, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000330 DAWDXZ010000000
VERSION     DAWDXZ010000330.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9929_length_2048_cov_11.873056"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          510 a          550 c          536 g          452 t
ORIGIN      
        1 tcgtcgaccg catcgtcgta gggtacccgg aagattccat tgataagatc caggctgaac
       61 tgggctatga agacgctctc ctcgaccagg ctgaaccttt ttccctgtgg gtcatcggag
      121 attcaaccct ggccgaccgc ctgccgttga aatcgagtaa attccacgtt gaatttaccg
      181 ataacatcca ggccttcaag gaacaaaaag tccgtatcct caacggggcc catacgtcga
      241 tggtcctcgg tgcgtacctt gccggcctcg actacgtcgg tcagtgcatg gaagacccga
      301 ttatccgcaa atccctggac cagacggtct tcgatgaaat cgtaccgacc gttgatttac
      361 cggaagaaaa ggccgttgcc tttgcccgag ccgtctacga acggtttgaa aatccctttg
      421 taaaacacgc cctcttggct atttccctga actccatctc gaagtggaga gcccggatcc
      481 tgccgacctt taaggacagc ctggcagcga cgggaaaact gccgaagttc ctgacttatt
      541 ctttggcggc tctactggcc ttctaccgga cgagtgaaca gggtgaccag tgcctcatcg
      601 ggtcccgggc cggcaatact tatgaaattc acgacgatga agataaattg gccttcatca
      661 aagatcatgc ctccctttcg acggccgatt acgttaaagc cgttctcgga caggccgatt
      721 ggtggggcga agatctgaat gctgttgacg ggctcaccgc tgctgtaacg gcccatatgg
      781 accgtatggc tgaggtcggc gtcaaagctc atcttgctga attgactgca taaggagatt
      841 atgatgaacg tatttcagat acacgctgat gataatgtag ccgtcgctgt cgacgctgtt
      901 gccaagggca gcagcgttac cgtcggcgga gcgtctttca ccgtctgtca gggcatcccg
      961 gcaggccata agatcgccat caaggatatt aaacaaggtg aagatgtcgt aaaatacggc
     1021 ttccctatcg ggaccgctaa aatggatatc cctcaagggt cttgggtcca tacccataac
     1081 gtccaaagta aactgggcga cctcctggat tacacctacg aaccggaaaa ggcccagcgc
     1141 ccgcctttgg aaagtgaaaa aaaatacgaa ttcctcggat ataaacggac ggatggtcag
     1201 gccggaatcc gcaatgaagt atggatcatt ccgaccgtcg gctgcgtcaa caacattgcc
     1261 cgggccattg aaaatgccgc ccaggtctat aagacggaaa acatcgacgg catctattcg
     1321 tacagccatc ctcatggctg ctcccagctc ggtgacgacc agctccatac gcagaaagta
     1381 ttaagtggcc ttattcacaa tccgaacgcc ggagccgttt tggtacttgg tcttggctgt
     1441 gaaaacaacc agatcagttt gatgaaggac gtcatcggcg actatgatcc ggaccgggtc
     1501 aagttcctca tctgtcagga cgtagaagat gaaatcgaag ccggcacgtc gattgtcaaa
     1561 gaactgtgcg actatgcggc taagtcccac cgggaaccgt gcgatgcctc cttgctgacc
     1621 atcggcctga agtgcggcgg atccgacggc ttttcgggta ttacagccaa tcccctggtc
     1681 ggtgaaatct cgaaccgcct catcgcagct ggcggcacgt cgattttgac ggaagttccg
     1741 gaaatgttcg gtgctgaaac gctgctcatg aaccgggctc ggaatgaaga agtcttcgat
     1801 aagaccgtag ccctggtaaa caactttaaa gaatacttca tgaactacgg cgaaaagatc
     1861 aatgaaaatc cgtcaccggg caataaggcc ggcgggatta cgaccttgga agataaatcc
     1921 ttgggctgcg tacaaaaagg cggtcaggcc ctggtcgaag acgtcctctc ttacggcgat
     1981 cgtgtttcca agaaaggcct gaacctcttg caggctccgg gcaacgactt ggtagcggcc
     2041 aatgcctt
//
LOCUS       DAWDXZ010000331         2045 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9937_length_2045_cov_4.453266, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000331 DAWDXZ010000000
VERSION     DAWDXZ010000331.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9937_length_2045_cov_4.453266"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    274..320
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1582..1698
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          484 a          541 c          430 g          426 t
ORIGIN      
        1 ctttccgccg gcacctacaa tggccgtaat tccaggctgc agcatacgcc gccaacgatt
       61 atctttaaac atgaatgaac ccccttttat acgcctaatt tgcaatatga cggctgtatc
      121 ccttcgaagc cgctttaata agactgcgaa ccatttcaat cgtcaaactc cgccgttccg
      181 cttccgagca ggtaccgaaa ttcccaaaat ccagttcttc actggctaag tgggctacat
      241 tttccttaac cgccatgccc ttttccgtca attnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn ccgaccgggc aatggcagct gctccgggac ggccgccgac
      361 gacgatgcgc catgaatcat cccgaagtga cgctgcgcca accaaataag gttcatccga
      421 cggcagccgc cgataggcct tataagcagt atacacttct tctcgggcaa tggaaatacc
      481 cgtaaccaat tcgcccatgg gagggcactg caggtaatct tctaaactca tgcgcccctt
      541 atcctggagc agcacgtcga cgtgcagaga cagaagaatc ggcagcagca cggaaaaagc
      601 acctttccct gaaataacgc cgccaagagt tccgtagcgc tgaatatctt tatcggcaat
      661 atcactgatg gcacggctga cggctccacc ggccaatttc tgaatgaccg gggaaatctt
      721 aatctgccat aagggcgtca tcgcgccgat atcgatttca tcgccttcct cttcaatgta
      781 gctgagcccg gccttcgata aatcaacggc caactcataa tgctcttttc tatatttcaa
      841 cccctgcgtt cccgctaaaa aaacagcgga cgcagcctga cgataggcat cataggcagc
      901 agccggacta tctggaacga gcgtcgtcgt actggtaatc acaatcgtcc atccaccttt
      961 ctttaaggcc ccgtatcgtc ctcgagggcg atattcggcc gatctttatt gagcaggaaa
     1021 gaaccgccgc attcatcgag aaccgcatcg accaagctgt caatgccaat atcgctgcac
     1081 agatccattt cctccttata taaaaggata ggcatgagaa gttccactgc cgtcccatcg
     1141 ggcatcttaa cgcctcccag ctgtccttca aactgaacga tgacaatgcc ggacagcgct
     1201 gtatcctcag caaaagactc cggtttttcc cgattgggca gcaaaaagcc ataccccatc
     1261 catacgtcgt gaacgtgggg aaagcggcct aattccatga gcaaccaaat cggccaggcc
     1321 gccttatcat tcagtgacag gccccgctct cgccgaaaag gccagcgagc cggaaggatg
     1381 agacacaatt cactgtagct ttcgttcgcc gccgcaaagt taccggacgg atagcgcatg
     1441 ggcgccgcac tcatgcccat ggtatgaagc aggtaaaatg gctcttcctc cgtcgggtac
     1501 aggatttcta catcgatgga cagcggtgtg ccaatgggat ctcgaaaaac ctgccagcgg
     1561 cggtgaggaa aaaggcggtg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnntg ttggacagcc tgtccgtatt catggtgcag cccattaatt
     1741 ttttttgtat ccgttaagga aaaaaatcgg ggccgacagg cggtctgtaa cggtaaggta
     1801 ttcatggaat ctcccccctt tgcgttatac tcaacttcat tataacataa atattccgtc
     1861 ttttggcaat caaaaaaggg gcggcgcaaa tgcgaagccc cctctttcac gattatgacg
     1921 ataaccgttt cagagtcgcc agcctccttg tcggagagat tccctgcgga ccgtatcgcc
     1981 tgttcaattg acgggatata tcatagcaac aaacctcaaa attgtcaact cagagggccg
     2041 caaac
//
LOCUS       DAWDXZ010000332         2041 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9964_length_2041_cov_6.013092, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000332 DAWDXZ010000000
VERSION     DAWDXZ010000332.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9964_length_2041_cov_6.013092"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1147..1156
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1712..1721
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          542 a          538 c          552 g          389 t
ORIGIN      
        1 tgatgcggcc gtgacggtcg ataaaattga tattccagcc gatgacagcc ttcgccgaat
       61 cgacgatttg ctgagccaat tcatcggtaa tgcggacctt catgtcccct caccttcttc
      121 cgatacggcc ggtcaggaca gggctacgtc catggccatc atcaccgtaa agcccagggc
      181 aaaggacaag acgccgatat cagagtgctc gccggccgac atttccggaa tcaattcttc
      241 gacgacgaca tagcacatag cgccggctgc caaactgagg agatacggca agacaggcag
      301 gataaaagcc gtaagagcga tggtaatgac agccccgatt ggttcgacga taccggaaag
      361 gacaccgccg ataaaggcct tatgggatcc cattccggcc gacttcaaag gcatggaaat
      421 gatggcccct tcgggcatat tctggagggc aatgccgagt gacaaagcca aggcaccggc
      481 ggcactgata ccggtctgat cggcgaggag cccggcatag acgacgccga cggccatccc
      541 ttcagggatg ttgtgcaaag tgacggccaa gaccagcatc gtcgtccgct gcaaatgact
      601 gtgcggccct tcggcctctt cactgtacat atgaagatga ggaacgaggc ggtccaaggc
      661 caagagcaga aagatcccca gccagaagcc gacgacagcc ggcacgaaac caaagcggcc
      721 taaatgggcc gattcttcca tggccggaat gaggagactc caaatcgagg cggcaatcat
      781 gacgcctgag gcaaagcccg tcaaaccacg ctgaacctgg tgattcaatt cccccctcat
      841 ggcatagacg caggcggccc ctaaggaggt cccggcaaaa ggaattaaaa tgccaataat
      901 ggcactgttc attgtactgt ccatagtaaa acccctttca tttcctatat ttttcctttc
      961 attttaccta tctgcggccc tcctgtcaat gaccctaaag gcgttgaaaa tgggattaat
     1021 ccgcaatttc cagccgtttt catttcgctc ggctcggcgg aaaggaaatc acaaacggga
     1081 cagcaaaaaa gactataatc atcgtgacgg gtgcaacaat aaattatagt ctttcctgac
     1141 ttcgatnnnn nnnnnnaaac ccaagtttca ccaaagatgc aaatcgctat ggacaagggg
     1201 cttgaacact tcgtctcata ggcctgaata tccctcgtgg gaagccctgg ttttatctga
     1261 aagcttggat ttaggttact attcactggc taaggcactg tcaccggtaa tcttgccggg
     1321 aaggaccaaa gacaaggcca tgcacactaa cgccggtgcc agccagccta agccgctgtc
     1381 ccctaagggc agtccctgaa tcaacgtccc tgccgaccgg acaccgaaga actgataggc
     1441 gatgtcgatg gccgatacca tgccggctgc cgctgcggtg cagcgcatgc ctaacttcga
     1501 caggctgacg aatttattga gaatggtcag catgacgacg acgatggcaa tgggatagac
     1561 gaagagcagc aaaggcaggg taatctgaat gatctggttc agacccaaat tggctaacag
     1621 gaagctgacg acacagatga gggtgacgta ctgcttatag gacaggacgg taaattcttc
     1681 ggcaaagaag gtcgaaacgg cagcgatgag tnnnnnnnnn ntaggcgccg aggttgacgc
     1741 cagggtcgga atagaccgac tggggaatgg gaaaatgatt gccgaggagg gccagcccca
     1801 ggtacaagca gcccatgagg acggcaacgg cggcgccggc catccagaca ctggccatgt
     1861 gttccttttt cgaagtgaac tgaatccgct tcaaggcgcc gatggcgatg atgcagaagg
     1921 caaaggaagc cagggtatcg acggtattat agccttcgat aaagccggtg ccaaaggccg
     1981 aggccgcgta ttttcccatg ggaaccgctt ccgacggcgt cgtataggcg atcatgccga
     2041 c
//
LOCUS       DAWDXZ010000333         2040 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9967_length_2040_cov_9.369270, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000333 DAWDXZ010000000
VERSION     DAWDXZ010000333.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9967_length_2040_cov_9.369270"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    388..397
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          480 a          515 c          575 g          460 t
ORIGIN      
        1 gcgctatttt ggcctgacgc cgttggtcat cggcccgggc gtcaagaccg gcatggatat
       61 caaatatgac aatcctcgtg aagtcggtgc cgaccgcatc gtcaatgccg tcgcagccta
      121 tgaaaagttc ggcggtccgg tcatcatcat cgacttcggg acggcgacga ccttttgcgc
      181 cgtcgataag aagggaacct acctcggcgg ggctatctgc ccggggatcg gaatctcaac
      241 ggatgccctc gtacagcgta cggctaagct gccgcgtatt gaagtcgtcc aggcagagaa
      301 agtgatttgc cgcaataccg ttgaaagcat gcaggccggt gtcttttacg gtttcgtcgg
      361 ccaagtcgac ggcatcgtta cccggatnnn nnnnnnnatc gtagcaccgg cgacggacgc
      421 catcgatgtc gtcgaaccga tgctgacgct ggaagggctg aaaatcattt atgaccgaaa
      481 caattaagcc ttttgccatc ggtaatgtcg tcctcgacgc gccgattatt ttagcaccta
      541 tggcaggggt ctgtgatgcg ccgtatcggg ttattgctca ccgctatggc gcagctttag
      601 tctgcgctga aatggtaagc tcccagggca tccgctacag caatcagcat acggcagaac
      661 tgctccacat cgaagacggg gaacaccctt tgtctatgca gattttcggc tctgacccgg
      721 acgtcatggc cgaggccgct gccgtcgttg aagaagccgg tgccgatatc gtcgatatca
      781 atatgggctg tccggtaaag aaagtcgtta aaaacggcga tggttcggct ttgatgaagg
      841 acctgccgtt ggcagaaaaa attatacgcg ccgtcgtaca ggctgtgtcc attcccgtta
      901 ccgttaagat gcgcaccggc tgggatgacc attcttttgt ggccccggaa ctggcaaagc
      961 gtgctgaagc tgccggtgcg gctgccgttg ccgttcatgg tcggacgcgg gagcagtttt
     1021 acagcggtaa ggccgattgg cagaaaatca aggccgtcgt cgacgccgta tcccttccgg
     1081 ttatcgggaa tggggatgtc atcgacggcc cgtctgcagc tgccatgatg aaagaaacgg
     1141 gatgccaggc cgttatggtc ggccgcgggg ctcaaggaaa tccttggatt ttcccacaaa
     1201 tacgccatta tcttgaaacg ggagaagtcc tgccgccgcc gactcctgaa gagaagtaca
     1261 cccagatgtt ggctcacttt gaagcccttt tggcttataa gggagaatac atcggtatac
     1321 gggaaatgcg gactcatgcg gcctggtata ctcacggcct tcacggggca gccgcccttc
     1381 gggagcggat caaccggacc cggtcggctg aggaatttcg ccttgtcgtc ggcgagatga
     1441 tggaccggct gcgcggacgg tgaagccgcg gttttgttga cataaaagac agatgtgaat
     1501 ataattaaag atacggtata aattaagcta taacagtgag acgcgattct atcgcaggag
     1561 gaataggaag acaatgacca atgaaacgct gattacccaa gaagggttgc agaaattaaa
     1621 agatgaatta aatgaactga aatcagtccg ccgtattaac gtttcgcagc gtattaaaga
     1681 agcgattgcc ctcggtgact tgagcgaaaa ctcggaatac gatgatgcta aaaatgaaca
     1741 ggcctttatc gaaggccgta ttgccgaatt ggaagtgtcg ccggccgcct gatggccatt
     1801 cgccgtcacg gtaagaccgc cttctgcgtc cttcgtgatt tgagcggtga aatccagctc
     1861 tacttccgta aagacgtcct tggagaagaa tcgtacaact tatttaaact cctcgatctc
     1921 ggtgatatcg tcggtgtcgg cggtacggta ttcgttaccc ataccggtga aacgacgatt
     1981 cgcgttgaat cgtggcagct cctctcgaaa tcgctccgtc cgctgccgga aaaattccat
//
LOCUS       DAWDXZ010000334         2037 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_9983_length_2037_cov_9.940969, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000334 DAWDXZ010000000
VERSION     DAWDXZ010000334.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9983_length_2037_cov_9.940969"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    947..956
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          517 a          499 c          488 g          523 t
ORIGIN      
        1 gtccctatca tatttctgcc cgtcaaacca aaggccgacc aaggccgttt cattggcagc
       61 caaggtcata gcccctaaag gcgacgcata actcatcgta tagtacatac atattctcct
      121 atcttgctca ttacaaaccg tagtacgatc tgtaccattc ggcaaattgc cccagtcctt
      181 cttcaatcgt cgtttccggc ttgaaatcga aatcacgctg caattcactg acgtcggcat
      241 aggtctgata gacgtcaccg gcctgcatgg gcagatattc tttttccgcc gtcttaccaa
      301 gggcttgttc caaaacttca atgaagtgca tgagctgttc cggcttatgg ttgccgatgt
      361 tatagacttt gtatcgattg cccgattcat tggccacagg cggattggaa agcatgtgct
      421 ctataccggt gacgatgtcg tcgacgtagg taaagtcgcg gtacatgtcg ccgtgattat
      481 aaatttgaat agcttcccca ttgcggatct tattggcaaa cttgaaataa gccatgtccg
      541 gacggccgta aggaccataa acggtaaaga agcgcaggcc cgtcgccgga accccataga
      601 gatggctgta agtataggcc atgagctcgt ttgacttctt cgtcgctgca tagaggctga
      661 tggggtgatc gacgttatcc gtcgtcgaaa aaggcgtctt ttcctgattg ccatagaccg
      721 acgaactgga ggcaaacaac aggtgagtaa ccttatggtg ccggcaagct tcgaggatat
      781 tgaagaagcc gatgatattc gaatcgatat acgtccgcgg atggtcgatg ctgtagcgga
      841 caccggcctg agccgccaga ttgacgacga tatccggctg aaacgacgaa aagatttgtt
      901 ctacggcctg ttcatcagct aagtcgccct tcacaaaggt gaaattnnnn nnnnnnttga
      961 gattgtcgaa gcccaggacc gtcgcccctt tagccagcag ccgcttggcc aagtggaaac
     1021 cgataaatcc ggcagcaccg gtaataagga ctttttgtgc tgtatcaaag ggtttaaaat
     1081 ttgccatatt cagttctccc ctcttaatcg cggtcaaaaa gatcccgcat atatacttta
     1141 tccttgacat cggtcaattt atctgaccag cgattggaaa tgatgatatc gctcatctct
     1201 ttgaattcat ctaagtctcg aatgacgcga gaatggaaaa aggtttcttc tttcagcgtc
     1261 ggttcataga cgacgacttc aatgcccttc gccttgatgc gcttcatgac cccttgaata
     1321 gccgaagccc tgaaattatc ggagtgactc ttcatggtca gtcgatagac accgacaatg
     1381 cggggatttt gagcgatgag catatcggca atatggtcct tccgcgtccg attggcggcg
     1441 acaatagcag aaatcagatt ttgaggcaca tcctgatagt tggccaagag ctgctttgta
     1501 tctttcggca ggcaataacc gccataaccg aaggatgggt tgttatagaa atcaccgatg
     1561 cgcggatcga ggcagacgcc tttgatgatt tccttggtac tgagaccacg catttcggca
     1621 tacgaatcga gttcattgaa gtaagccacg cgcagagcta aataggtatt ggcaaagagt
     1681 tttatggctt ccgcttccgt agaccccgta aacaggacgg gaatatcttc tttgacggcc
     1741 ccttcgacta ataaattggc aaaggtcttg gcccgatccg acacttcgcc gacgacgatg
     1801 cgcgacggat ggagattatc gtagagtgcc ttcccttccc gcaaaaattc cggcgagaat
     1861 aaaatattat ccgtcccgta cttggcctta atttgctgtg tatacccgac agggaccgtc
     1921 gacttgatga ccatgacggc cttaggattg atggccaaca cttcctcgat gaccttttct
     1981 accgacgacg tatcaaaaaa gttccgctta ggatcataat tggtcggcgt cgaaata
//
LOCUS       DAWDXZ010000335         2029 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10034_length_2029_cov_6.528369, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000335 DAWDXZ010000000
VERSION     DAWDXZ010000335.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10034_length_2029_cov_6.528369"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1535..1544
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          501 a          569 c          519 g          430 t
ORIGIN      
        1 ggccgcggcc ggaagtcagt cgatgtgtat ggccggtaaa tcaagcgggt cttttggacc
       61 aatcagggtg gaaccgcggg tacgtatact cgtccctgta acatacttat ctatgttaca
      121 gggacttttt tgcatgtagg agggaatacg atgaattttc aagacatgat attcgcattg
      181 aagaacttct ggtcttctca gggctgtatc atctacgaac cgtatgatgt agaaaaaggg
      241 gcagggacca tgaacccggc gacgttcctc agaacgctcg gcccggaacc gtggcacgta
      301 gcttatgttg aaccgtcgcg ccgcccggca gacggccgct acggcgacaa tccgaaccgc
      361 ctttatcagc accatcagtt ccaggtcatc tgcaaaccgt cgccggacaa cattcaggaa
      421 ctctatttaa agagtttgga aaccttgggc atcaacccgg ctgaacacga catccgcttc
      481 gtcgaagaca actgggaatc gccgaccctc ggcgcctggg gccttggctg ggaagtctgg
      541 ctcgacggca tggaagtcac gcagtttacc tacttccagc aagtcggcgg catcgacgtc
      601 gcacccgtat cgtcggaaat cacatacggc atggaacgct tggccatgta tatccagggc
      661 gtagaaaacg tctatgacct ccagtggaac gaaacggcca cttacggcga catcttccat
      721 cagaacgaag tcgaacagtc gacgtatgcc tttgaactga gcaacgccga cctcttgttc
      781 cacctcttcg atgaatatga aaaagaagcc atgcgggtca tcaaagccgg cttcgtcctt
      841 ccggcctatg actacgtatt gaaatgctcg cacacgttca acctcctcga tgcccgcggc
      901 gccatcagcg tcagcgaacg agctgccttc atcggccgcg tccgcagtat ggcccgggcc
      961 tgcgctaagg catacctgga acagcgtgaa aaattaggat tcccaatgct ttccaaggag
     1021 gaaaaataag atgagcaaag atttgttact tgaaatcggt accgaagaaa tcccggctca
     1081 ttatatgccg agtatcctcg gccaggtcaa agacttggct gaaaaatcgt ttggcgaagc
     1141 caatatcgct tatgaatcgg tccgcacctt gggcacgccg cgccgcatcg ccttgctgat
     1201 gaaaggtgtc gctgaaaaac aggctgacgt ctcggccaaa cacaagggcc cgtcggtctc
     1261 tatcgcctat gacggcgacg gcaagccgac caaggctgcc atgggctttg cccgcggtca
     1321 gaagattgcc gtcgaagact tgaccgttga agacggttac gtctacgcca acgtcaccag
     1381 cgtcggtgct gaaacaaagg gccttttgcc ggacctcatg aaaggcatcg taacgggcct
     1441 caacttcccc aagagcatgc actgggggag cctcgacttc cacttcgtac ggcctatccg
     1501 ctggttcgtg gccctctttg atacagacgt cattnnnnnn nnnncctgga agaagtcgtt
     1561 tacctcgtcg aatatccgac ggccctctgt ggtgaattcg ataaagatta tttgaagctc
     1621 cctgaagcgg ccatcattac gcccatgaag gaccaccagc gctatttccc catgcgtgat
     1681 ggagaaggca agctcatgaa cctcttcttg accgtccgca acggcaatga ctaccatttg
     1741 gaaacggttc agcacggcaa cgaacgcgtc cttcgtgccc gcctcgacga cgccaagttc
     1801 ttctttgaag aagataagaa acataaactg gtcgattata cggaaaaatt gaagaagatc
     1861 gtcttccagg acggcctcgg tacgctccac gacaaggctc agcgcctggc acagatcacg
     1921 gtattcctta atcagaaact cgatctcggc ctcgacgatg ccaaactcca acgggcttct
     1981 gaactggcca aagccgacct tgccacccag atggtcatgg aatttacgg
//
LOCUS       DAWDXZ010000336         2028 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10038_length_2028_cov_7.278256, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000336 DAWDXZ010000000
VERSION     DAWDXZ010000336.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10038_length_2028_cov_7.278256"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1369..1378
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          464 a          532 c          536 g          486 t
ORIGIN      
        1 gaactatgac gccaaggggc caaaaataaa gaaagaaaaa cggcactgcc tcatagacag
       61 cgccgtcaga tgccgtcaat cagcaccttc gatactgttg aaatctttag ttcatagcgt
      121 cgttctggac cgatgtcacc gccgacgagg tcgtgtcgtc aagggcggct gcatacaatc
      181 tccggttcac agtctgtccg gacggttcag ccttcgccgt ttcattcgaa gcgtcgacgc
      241 cgggaacctg gacgacagac gtctgtccgg cagggaccat cgtttctaag cgaacccggc
      301 cggcaccgac gcggctggaa agttcagcca gcgtgtcctt acttacagga tgacgaacgg
      361 taacgacgat ctgttcatgg acgtagtccg gtacagcacc gacgtagttt tggacgcctt
      421 ccttatcgac gatggtatga atggcccggt tcatgagctg gctgtaatcg gcgtggctca
      481 aggaaacgcg gcggatttga acggcttcag gcttcgacac atcgtcctta atccgtttcg
      541 ttaagtactt gctcgggctg cctttgatgc cgagaactaa gacatcgtta tcccagtaca
      601 tcgtccctct ttggaaataa gccaggaccg aaccgtattc ggcattgatg gccttcatca
      661 cctgggtttc aatctgcacg tgagacgtat agtcgccgtc agaatgcatg aaccgataaa
      721 atacctgggc cgcatcggct cgggtcatcc cttctttggg tcggaaatag ccgtcggccg
      781 gcaccatgat attcttgctg tgaaggatgg cgacagcttg cgcataagcc ggagacacct
      841 gcgcttcgtc ggcataaggc atcacttcct gatccggatc ggccatgcgg ctatactgca
      901 ggtaacggta aatgacgtca gcgaactctt cacggcttac cggcgcttca ggctggaagt
      961 ttccgtcctg atagcccgtc aaaatacctt gagccgcaac ggcattcata gcatcaccgg
     1021 ctgtcccttg ggaaacatct tttaacgccg gcgctcccgg ctgaactaag gggatgtttc
     1081 cggcaacatt atacaggagc tgagctaatt caccgcgggt caggacccgg ttgggctcaa
     1141 aggagtttcc cgtaataaaa gtcaggcact gcgtatcata caagtaatcg acggcctggc
     1201 ggtcggccat actccccaaa tcggtgtacg gagcatcggc gaaagccggg cttgccgaaa
     1261 ggagcatcgc agctgccaag aaggcagcga ttttatgtcg taccatgaga gacctcctag
     1321 cttactgaat gacgatacga agggcactcc ccagcgcttt attgaggtnn nnnnnnnngt
     1381 tgttctgcgt ttctttcgaa atggaaggaa cggtgaggat aactttttca ttaaggtaat
     1441 ccggtgccgt cgtgactgca gcatccgtcg attccgtagc gcgataaatc ttttcggcct
     1501 gggccataat gttcttatag tcattgtagc tgtacgtcga cttctggaca tagaccgatt
     1561 cagcaggaat atccttatca tcggcaatgg catcggctaa tttctggcgg ttcttgacat
     1621 cttttacacc gatgtggagt ttgtcgccct gccagtacat gatgccgtcg ttggcaaaag
     1681 ctttgaccga accgtagacg tctttaatat tggtaaaggc cttagcttcc aaaccgtcct
     1741 ggctctggtt ggtcactttg acgccagtca tgatgcggta caagacgttg accgcttcac
     1801 cgcgggtgac gtactgtttc gggttgaaca tgttgtaatt gccgcccttc atatagccgg
     1861 cagcagccaa ggcatggacg gcctggcgag cccacgtcga aatcttggct tcatcggcaa
     1921 aaggagccgc accttcaaca gtggtcatac cctgataagt catatagtta taggcaatga
     1981 cggcaaattc ttcacgagtg atccgctgct gcggcttaaa ggtaccgt
//
LOCUS       DAWDXZ010000337         2027 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10045_length_2027_cov_6.423935, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000337 DAWDXZ010000000
VERSION     DAWDXZ010000337.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10045_length_2027_cov_6.423935"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1008..1017
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          564 a          354 c          547 g          552 t
ORIGIN      
        1 atgatgagtg gctgttgcga tttttgcaac aaccactttt tttgaatatg gaaaaagata
       61 aaaaataagg gacatgtcgt aacacgtccc atccaaaacc atatataata tgattttcaa
      121 gttgattgta gtataaaata ggacgagtta gttgtcaatc gttgggaaaa atatagaaca
      181 ggtattcttt ttttgcaaat gctgacagtt aaaatagctt caagattgca gaatgcgttt
      241 ttgtgattat tattatccta ttcagttata aaatggtatt ttacaactga atccttatcc
      301 agctcatatt ccttacagca ccgtcgtaaa tggtggggaa taatttttat tcagggggtc
      361 aaaaaggggt caaaggcatc tgacttttgg gggtcacata aaaatagcca ccgcacaaat
      421 gcgatggcta tcagctttct cgtatggtgg accatcaggg gatcgaaccc tggacaccct
      481 gattaagagt caggtgctct gccagctgag ctaatggtcc atgtttgttg ttggtgtgct
      541 gacctcgtga gcacatttgt tataatacac caaatgtaaa ataattgcaa gctttttttt
      601 gtgaattttt ccaaagaggc cgaaagccgt gataaatatg tcgaagcctt ccgcaatagg
      661 gcatttatta aaactttctg agaaggacct ctcagttggc aggataattc tgttttacag
      721 cgaaatatat aagtatcggt tgtaatgtaa gataaaaaat ggattcgtgt tcataaatac
      781 gttttgacat cggaagcggc gtgaaacagg gggctaaggg ctatttccca gtcttcgtgt
      841 tcgacggatg tctggcatta ttatagatat ataaagtgat gaaaggatga gcgtaatgga
      901 aagttcagat agaaatttaa agttatatga ggaggctacg gaaaagcttt gtgccggaga
      961 ttttgacgag gctttaaaat tatatgcctt gtctatgcac gacgaggnnn nnnnnnngtt
     1021 cgatgaaggg ctacaatgcc atgggaatca ccttttactt gaaaggagat gtttcgatgg
     1081 ctatcaagtg ctatgccgtc tgcagtaggt ttgcggccct aagtattccg gagattctgc
     1141 aggattatca ggccatgcgg aaaggggaca cagaggctaa agaccgttta ctggcccggt
     1201 gttcctattt ggcagccgat tggggctgga gtgagtctaa ggcgcaaatg gatgaaaaga
     1261 gtcggtccct gaatggaaat aatgagcagc tgtatcggga ccttttgatg ggaaaacggg
     1321 aggaagactc gctgtcaaat ggcgagaagc tttcatacag ccagtatctg ctggagtgcc
     1381 ggaaaatcgg ctatgacctc atttttcatg acttccaaaa catgatcgat gatcgggacg
     1441 gcacgactga agaaatgaaa caatggatcg ccgatatttt tgatgtcgtt aaaaagttgg
     1501 tcatggcggc cgataaggct gaataggaaa gatggtgtgg catcgctggt ggattgtctg
     1561 tcttgtatac cggaacaggg cgtgtgttcg taaaaaaact gtcggttgaa cgagcctttt
     1621 tcaggcctgt ttaaccggga atggagcgac atatggtgaa gggctggagt aagggtaaga
     1681 agatggaaat ggccgagaag gctgcgaaga ccgtggccgg cgtatcggtg ggagcggcgc
     1741 agagcggagt ggatttattc aaaaataccc gcggcgtctt ttatcgtcag aaagattttg
     1801 aagctttgct gaccggcatt gagcagcagg ctgtggctta tgcgacggtt cgagaggcgt
     1861 tggcagcggc tgagttatcg gtggaaacga gaaaaatcct attcctcgat tctctggggc
     1921 tggccacggg cagtatcttg agccgcctca tgggctggca tgtgccttct gatgtacagc
     1981 aggcctatga aatggcgtat ccgcaaaagg cggcggccat ggatttt
//
LOCUS       DAWDXZ010000338         2024 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10054_length_2024_cov_10.045201, whole genome shotgun
            sequence.
ACCESSION   DAWDXZ010000338 DAWDXZ010000000
VERSION     DAWDXZ010000338.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10054_length_2024_cov_10.045201"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          625 a          432 c          576 g          391 t
ORIGIN      
        1 tcaagtggca tcccaggagg agaagattac tcgagttctg ggtcatgaaa aaaatagaca
       61 gggccgaagc cgagcggatt ttttttggtg ataaggatga tgagcaatga gaacggtaat
      121 atcgcttaaa gaactaatgg agcgctggga cttgtctcgg acgaccatca tgaagatgga
      181 acaagacggg cgcctaaagc ggctcaagct gccggggata tattacccga tgaaagcggt
      241 tttggagctt gaagggctgg acccggcgga atggagcaag agcccattcg agtggcggcg
      301 gctcaaggac gagctggaat ccaccaagaa agaattagtt gcctgccggg aaacattgaa
      361 ccaattctcg gcaatattag ccaagtacaa ctatgaggag gcggctaaaa atggtagatg
      421 aaaaactacg ggcatgggta tggcgcggcc tgctgctgtc ctgcctgctg ttttggacgg
      481 cactgattgc ggcctgtatc aacatctaag gagggaaaac aatgaaaagc gaatggaaag
      541 tcacctacaa cggcgagtat tatgaagtct atcggctacg cgacgtgtca cgagtggacc
      601 attccggcaa ccgcgaatac gccaaaccga cgttcaaaat gaaatacctg gcgcagacct
      661 atgcagccga agccaatgat atagaagaac gccaggaggt gcgaggatga aacgcttaat
      721 agctatgctg attgttctgg cagttgccgg cggaacggcc ggctactacg ataggccgga
      781 ggccgaagag gccccgatag tccacgttgt agagcccgga gaaacgctat ggagcattac
      841 ccggcccatc gctgacagcc gcggggagga cgtcagagag acggtcgccc gtattatggc
      901 cgacaatgac atcgagcctg atgccgtcat ccggccgggg caaaaactta tcattaagtg
      961 agaggagaaa taacaatggg acaatggaaa gcccttgtta aatgggcaaa acaggagcgg
     1021 ttatataacc aactggcatc tgctaccaaa aaggctgagg acctagaatg ggatggggat
     1081 agtggagtta ttgtggccac aaacatcaag aaaatagatg ctgcacttat atatgccaag
     1141 ggcagccccg ctactgtcat ccagctcatc gccattatat taaagcgatt tgtgggggcg
     1201 tatccggaag aacagcaaaa ggaagttatc gaagatatct tgaaaatagt taatcaatta
     1261 taggaggtag aatcatgaac aaaaacgaac aagcacggct ggatgctgaa tttagggctc
     1321 actgccgcga agcactcaag gcccagaagg agacgcatgc cccaggaaag agcctaatcc
     1381 ccttgaacga ggcaatcatt aacgctgccg aggcccgcgg ctacgggcaa gcattagttg
     1441 acatgcttaa atggaagatg gatgcccccg aaatacgtag ccatatcaac gacttaattg
     1501 cagaggcgga gcaacagacc ggcataaaaa ccggcctgcg gttgacgccg tggaccgaag
     1561 aggaggcggg gagatgacgc acgaggaata tcaagaaatc attgcattag ccaaaaaggg
     1621 gtgttactta cgatgtaggt tatgatgagg ctgtagtgat cttagcggca tcggaaacag
     1681 aagacagctt ggtactggct cataacgggg atgctagtgt tatcgttccg acactccgga
     1741 gcgcacttgt tggaattatt aagggcgccg ataccgaaga taaaaaagtc tatacaaaat
     1801 cccttatcca agccattgca gaaattgggg ttgatgttac cagtgataca aaaaagtctt
     1861 gaaaaggagg tgaatagatg gatgctaaaa agctgcgaaa gcggctgcgg aaaatccaag
     1921 aagacatcga aaaagctaaa ttgggtgaaa acgatgctct tattttactg gcctccctcg
     1981 acgatgggaa agacgaggcc gaaatacacg caatacaggg ccgg
//
LOCUS       DAWDXZ010000339         2022 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10066_length_2022_cov_11.354855, whole genome shotgun
            sequence.
ACCESSION   DAWDXZ010000339 DAWDXZ010000000
VERSION     DAWDXZ010000339.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10066_length_2022_cov_11.354855"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          473 a          587 c          560 g          402 t
ORIGIN      
        1 gcgtccctta tatcgtcgct aaaggacgaa aagggggcag caccattgcc gctgctatct
       61 gcaacgccct catttataaa ctgactcgcg gataagccca tatcttactt acttcttact
      121 ggcacggcgc cgtcatgcgt agtataataa gggcgacatt taaatgagag ggagtgtaga
      181 gtatggaaac atggcttgat ttgcacatgc atacaaaata cagcatggac ggtcagtttg
      241 aaccggccga cttgatacgt cagtgcgctg acgcgaaggt acaggccgtg gccgtcaccg
      301 accacgattc cacccgggcc attcccgaag cacgggaaga agcgtcccgg atgggactgc
      361 gcctcatacc gggcattgaa atcagttgtc agcatgaggg gaagaacttt cacctcttag
      421 gctatgggat tcatcccgat gccgccgtat ttgaagcgat tgaaaaggat gtgtacggcc
      481 aacgaaaagt cgtgtccgac aaagtgctcg acgccgtcga agccttaggc atcgtcctcg
      541 accgcgacgc catttggaaa ctttgtacca gtggtgtcgt cgcgtcggtt cacatcgctc
      601 gcgttgccct ttcggacccg cgcaatgccg actgccccgt cctggcaccc tatcggccgg
      661 gcggcagccg cagtgctgcg ccctatgtca acttcggctg ggatatctgc ggtcaggggg
      721 gaccggcctt catccccatg accttcatgg atttttcaaa ggctgtcgat atcattcaca
      781 atcagggcgg cgccgccgta ttggcccatc ccggagccaa tatgaagcag aaccgtcctt
      841 tgacggaaac cctcatcgcc ctcggtctgg acggcatcga agcttactgc agctatcacg
      901 atgacgatac ggctgccttt taccgccgga ttgccgatga acacggcctg atggcgacca
      961 ttggcagcga ctaccacggc cgggctaagc cgcatattgc cctcggcacg tacggtcatc
     1021 ccgagccgca ggctttgtgg gaccgcctgg tcgcacacat tcaaaatcat catggggaag
     1081 tgtactaaat catgaaacaa agcaagaccc tgtcagccct ggccgttgcg ctgtggctgg
     1141 cttttggttt ttcaagcctg tcgttggcgg catcgccgtc ggacatcgaa gcggcctata
     1201 cggtcggtgc cgtttccgat tcgaatcagt ccgtaacgaa ggcggctact gaaaaaggaa
     1261 aggcgacggt aaagaaaaag ggcgtcgata aaaaatcgtc gtccgaaaag aaggggacga
     1321 aaggggatac ccctgccaaa tctttgacag acggcgaatc ctcagaactt cagcccgtcg
     1381 ccgtgagcag tgtaccacgg gatgcgaagc ctgccggagc ccgcggcgtc ctgcggacct
     1441 atcatccggc aacggatgca gccccggccg aaccgatggc tattaaggaa acgaatttcc
     1501 gcttcaatga acccttattg ctgcggccca agacggacat gatcgtcatc caccacgtcg
     1561 gcattcccga cggcgatacg tcggcggctg ccattcaccg agcccattta gcgaacggct
     1621 gggccggcat cggctaccat tacgttatcc gcaaagacgg gaccatcgaa cggggacgcc
     1681 ccttagcgac ggtcggagcc catgcccaag ggcagaacta tcacactgtc ggcatcaacg
     1741 tcacgggcaa cttcgataaa gaaattccca cgccggctca gctccacgcc ttagaaggcc
     1801 tcgttgcctg gttgtgcaag atttatgaaa tcacgccgag tgcagcgacc atcgtcggtc
     1861 accgtgacgt caacagtacg gactgcccgg gtcagcacct ctacgacatg ctgcctcaga
     1921 tccgccgcga tgtggaaaag cggatggaag gcgatgccgc tccgtcttat gaagataccc
     1981 tgaaagaaaa gagcctcctc aagatgagtc gtaaggaacg gg
//
LOCUS       DAWDXZ010000340         2022 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10067_length_2022_cov_10.107778, whole genome shotgun
            sequence.
ACCESSION   DAWDXZ010000340 DAWDXZ010000000
VERSION     DAWDXZ010000340.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10067_length_2022_cov_10.107778"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          478 a          532 c          513 g          499 t
ORIGIN      
        1 aatggacggc gttcccgtac tcgacaaggt agccgctact ttgcgggcaa tatgccctga
       61 ttttcccatg ccggtgacaa tgacccgacc gtgggattcc atgataagat tcacggcatt
      121 gacgaagttc tgatccaatg tcggaattaa tttttcaatc ccctgtgctt cctgatggag
      181 cacgtctctt gcttcttcta aaatatccat catatcggct cccctcacat ttccgtactt
      241 acgctgtttt tggcaatgtc gcgaatggcg accatatcgc gaaggacctt ttcgacctgc
      301 gacaaataca gcatattcgg gccgtcgctg agcgcttcgg ccgggttgtc atgaacttcc
      361 atgaaaacgc cgtcgatgcc ggaaccggca gcagcacggg acagattacc aatgaactgg
      421 cgctgaccgg acgatttggt accggcaccg cccggcagct gaacgctgtg ggtcgcatca
      481 aagacgacag gatagccgaa gctgcgcata atcgggaagg aacgcatgtc gacgaccagg
      541 ttatgatagc ccaggctgac gcctcgttcg gtgagcatga tattttcatt acccgaatgg
      601 acaatcttat cgacgacatt cttcatgtct tccggtgcca aaaactggcc tttttttacg
      661 ttgacgcatt tacccgttgc agcagcggct tcgaccagat ccgtctgccg gcagagaaag
      721 gccggaatct ggatgacatc caggacttca gcggccgctt ccacctggtg gacttcgtgg
      781 acgtcactga cgacgggaac ttgaagttcc tttttgatgt cccctaaaat acgcaggcct
      841 tctgtaaggc ccggtccgcg atacgacgta tatgacgaac gattggcctt gtcaaaagag
      901 gctttaaaaa tatagggcag gcccagtcgt tcgcaaattt ctttcatctt gcggccgatg
      961 ttgagcgtcc gttccggatc ttcgatgaca cacggaccgg ccatgacaaa gaggctgccg
     1021 ttgccgccga cgatcgtatt gccaacatga atttgtttca ctgtttccat agtctattcc
     1081 cctttatgag cagattcagc aaaataacgg cggatgcgtt caagatcatc cggcgtatcg
     1141 atgccaatga attgatgatg cgtttttatc acttttatct tataaccatt ttcgagaact
     1201 cgcaactgtt cgagggactc aactttttct gccggggtcg gttccatagc ggcataggcc
     1261 aagagaaagt cccggcggta ggcataaatc ccgatgtgtt tgtatggtac cacgccgccc
     1321 tcttcaaaag catgccgcgg atacggaatg agggaccgtg aaaaatacat agcttcgtcg
     1381 cgctgattga ggacgacctt tacggcactc ggttcatgat attcttcttc agtcagaggc
     1441 gctgcaacgg tagccataga aagatcgggc tcctcttcaa aaacccggca cagatcatcg
     1501 atgacgtccg gcgcaatgag cggttcatcg ccttggacgt taatgatgac gtcggcatcg
     1561 ggaaagtgag ccacggcttc ggccaagcga tcggtcccat tggtatggtc ttcccgggtc
     1621 atgacggcat gaccgccaaa ggaaacgaca gtgtcataaa ctttaggatt gtcgacagcg
     1681 acgacgacag ccgtcagttt cgaggccttg gacgcctgtt catacacgcg ctgtaccatc
     1741 ggtttgccgg caatatcaca taaaggcttc cccggcaggc gcgtcgaagc gtagcgcgag
     1801 ggaatgatgc atacaaaatt catagtttat cctccaatac agattgcaag acggcatcta
     1861 ttttttcttg cccttctacg atatcaaggg taatggccaa gacgtacaag ggaaggtcgt
     1921 attcttttac aagatcggcg ggaagcttca cggcatcttt ttctgtcgtc accagaatac
     1981 actgcttttc ggcagccatg tcggccattc gccggatatc cg
//
LOCUS       DAWDXZ010000341         2016 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10102_length_2016_cov_6.077511, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000341 DAWDXZ010000000
VERSION     DAWDXZ010000341.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10102_length_2016_cov_6.077511"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          445 a          522 c          547 g          502 t
ORIGIN      
        1 ggccacggcg tcgatgcgaa ggccgtcgat atggaactgg ctcagccaaa agagggcgct
       61 ggaaatgagg aaggattgga cttccgtccg gccgtagtcg aagttcatcg tatcccattc
      121 cttgttttcc gccaagatcg gatttcccga ttcgtagcac ggttcgccgt cgaagtggcg
      181 caggccgtgg gcgtcgcggc aaaagtggcc cggcacccag tcgaggatga cgccgatgcc
      241 gtttcggtgg cagtggtcga tgagatacat aaaatcttcc ggcgtgccat agcggctggt
      301 cgctgaaaaa taacccgttc cctgatagcc ccaggaaccg tcaaagggat attcacacag
      361 cggcatgagc tcgatatggg tataatgcat gtcttttacg taagcgacca gctcttcggc
      421 gatatcgcgg taagtcagca tgccgccgtc ttcgccgcgc cgccacgagc cgagatggac
      481 ttcataagtc agcatcggtt tacgatacga atcgtaagcc tcacggctat ccatccattt
      541 cttatcgtgc caggtatagt ggagcttggt cagtttcgat gccgtatgag gccgtacttc
      601 cgaccagaaa gcgtaagggt cgctctttaa gatgcgttct cctgacgccg tcgtaatggc
      661 gtatttataa atatcgcctt ctttaaggcc ttctatatat gcgacccaaa tgccattgcc
      721 ttggctgctt cgttccatcg gattggcatc gtcatcccaa tggttgaagt caccgacgac
      781 actgaccttc tgtgcatgcg gtgcccaaag ggcaaaacgc acgcaggatt tccggcgcca
      841 ccctgtaaag tgagcgccga acagtcgata gctggcataa ttcgtaccct ggtgatacaa
      901 gaaggtactg tattcgtcga ttgcactgag tttcatcgca tatccccctg tctttcagtc
      961 gtgcttagat ggcttgatgt gccaaatatc gtcggcgtac tgcttgatcg tccggtcact
     1021 ggagaaatat cccgactggg caatattgac gacactggac cgccaccaat ctgatttatg
     1081 tccataccgc ttggcaattt cctgctgtgc ctcacagtag gctttaaaat ctttcaagac
     1141 gaagtattcg tcgttcttat cgaggagtga ttcgtagagc agaccgaaac tttcaccgcc
     1201 cagagttccg tcgacgaggg catccatgag gcgccgtata tcggaatcac tgctgtacat
     1261 cttccaagac gaataaccgc cgcgaatata gtaattcatg acttcatcgg ctcggagacc
     1321 aaagatgacg cagttgtcgt caccgacgag atcgtggatt tcgacgttgg ccccgtccat
     1381 ggtcccaagg gtaatggctc cgttcatcat gaatttcata ttccccgtcc cggacgcttc
     1441 cttgccggct gtcgaaatct gttcgctgac gtcggcagcc gggaaaagga gctgtcccag
     1501 agatacgttg taattttcca ggaacaggac cttcagctgc tggctgaccg tccggtcgcc
     1561 attgatcatg cgggcgatga cgttaatcag gcgaatcgtt tccttggctt cgccgtaact
     1621 ggctgcggcc ttgccgccga agagataggt ccgcggggta atggtcaggg acggattgtc
     1681 ctttaagagg aagtagaggt gcaggatatg gaggatattc aaggtctggc gtttgtataa
     1741 atgaatgcgc ttgatctgaa tatcgaagat ggaatcggtc cggatatcct gattatactt
     1801 gtcccgaatg tagcgggcca gccattcctt gcgttcggtc ttgaccttgg ccagcttttc
     1861 cttaaaggaa gcgtcgtctt tataggccat gaggtcggcc aaacgcgacg gcgtcttacg
     1921 ccacttgtca ccgatcgtat cgtcgatgag ggacgccagt ttcggattgg cctcgatgag
     1981 ccagcgccga tgagaaatgc cgttggtctt attgga
//
LOCUS       DAWDXZ010000342         2014 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10113_length_2014_cov_6.055641, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000342 DAWDXZ010000000
VERSION     DAWDXZ010000342.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10113_length_2014_cov_6.055641"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    765..872
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          455 a          493 c          363 g          595 t
ORIGIN      
        1 attttattca cctgatggtc gcctagtccc agtggatttt gactgacaat tatcttgatg
       61 tccgccaaat ttatcccctg tacttctctc ggcaaggctt tttttaaaaa atcttctgcc
      121 ggcatatatg tatctgaaac tctgattttt ttcactatgc tcatcacgta tcatctccta
      181 ataaatcacc atgattttca cgctctttgt ccaaatcctg ttgtaaatca tcgcgtataa
      241 attttaaact cccaatctcc tcatcacgcg ttgcgatggt agccttagcc tcttctaatg
      301 agttctttag ttcctttatt ttatcattca gttcgtggat ggtactgctc cattgacttt
      361 tttcattgaa gaactgatta acttgattgt tcaattcttt attttcttcg gctaaggttc
      421 ctctgcttct ttcctcttca aattcggctt tttccgcctg atacgcctct atgcgttcat
      481 ccaatctatg ttcccgtacc ctaagttctg aatctttctt attgtacgct gcttcctgct
      541 cctcttccca cgctgccagt tcagctttcc gatccgacag gcgctgttct tcctcttcca
      601 atccttcttt caggctatgc aattgagccg tcatagagga aagagaagta tccttttctg
      661 acagtgcctg atgaaaactt tcctgctgtt cgttgagggt gcgttcaaag aggtctcgtt
      721 cggctgcata gtttgcctgc tgcgccgtaa gagctcgttc aaaannnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntctttttc tttcaattgc tgttgaaaaa
      901 tcgactgctt ctcttgcagg ctgtccactt gataacttaa cgcttgatta tctcgagtat
      961 attcctcaat aatctcctgc aattgttcca aaatggcctc acaatctcga acaatttttt
     1021 tcccggattt atagatttcc tgtgctttat tatttcccat attcgtcttc ctccatcagt
     1081 atatagctat agagccgatg tgcatggtcg gctcatgacc gatgatgcca aaatgacgct
     1141 gccaaaagac ttttgtctcc aacagccaat ctaaaaacag cagctccaag agccttactt
     1201 ttttttcatt gatttccgac ttcgctgcag gaatatacac caagaactca tcgccttcct
     1261 gataatagac gcgggcttcg gggaaaaaac gctgcaaggt gagtcgatag acacttcggc
     1321 cgctcatcgt tccctcttca tgctggagca cagcctgtaa gagtatcgct ttttcatcag
     1381 cggtcaggca cttatagccc tgctgcacct cttgtccata gcgaccccgg cgaatatcct
     1441 cccctacggt ctcccgtttt aaagccccgg ccgtcacgcc ttgcaggcgg tctaagtgag
     1501 ccaagtaatg aagaagccaa ttttggatat ctttacagac agcctcgttc agcccctccc
     1561 ggggctggca taaaggccgt aatatctcag aaaaacgcag caggggattg acctgtacaa
     1621 cctgtcctcg ggtactaaaa acctctacat agggagatac agggttcgta tcgacgatat
     1681 atcgcttttg cttgtcataa tcctgccaaa tctgactcat tgtaaaacac ctttccaaga
     1741 aaaatctggg aagcgccgcc ggagatcggc cagtacgtat acagcatagt ccggcaagaa
     1801 atccctttgc cagtctaaag cccggggatc caagaaaaat tgtatatagc agggaacgag
     1861 ccgctttaat cgttcttgct gatataaaat atcttcgcga ttgccgccat acatatcttc
     1921 ttcccgatat agacgaacca tctgtccttt ttcgggggct ttcaaagaag gacggccaca
     1981 gcaaaatcct gaggattgat agcggtgaag agct
//
LOCUS       DAWDXZ010000343         1993 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10226_length_1993_cov_6.341589, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000343 DAWDXZ010000000
VERSION     DAWDXZ010000343.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10226_length_1993_cov_6.341589"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          525 a          553 c          405 g          510 t
ORIGIN      
        1 acgacgccgg cggactgacc agtgcaataa agggaacgta tcccgattct tgtacggaag
       61 actgccaatg cttcaccatt ccgatttttt cagctgcttt tcctcgaatt tcttccaaca
      121 agtccatgac ttccttattg gcttcgagca ttttaggcgt ttccagaccg gtcagattga
      181 gttcagcggc ttggataaag accaagggat tgccgacatc aaagatggaa acgcgataat
      241 tccgaccgcg ggcttcaatg acatcggata aatttccggt aggcagcatc tttcccgtaa
      301 aggctccctg accgtctttc cagtctaagg tgatgcgggc cccgaacccc ggcacgccgt
      361 caatatgaag ctctccgtcg acaacggctt cgccttcaga aacgggtatt tccgcggtga
      421 cgatattgtt agaattcgtc aagtggatcc gaacggttgt caccggttcc gttaccggaa
      481 ccaatccgtg atggatggca aacggaccga cagcagctgc gatattgccg cagttcccct
      541 tgcggttgac actggcttcg tcgagtgcca cctgagcaaa ggtataatct acatccgcat
      601 caggccgtga cggcggaccg atgatggcac atttgctggt cagtaggtca ccccctccca
      661 gcccgtctat ttgacggata tccggtgtcc cgaaaatctg taagatgacc ttatcctgct
      721 gatcccgatc cgacggcaaa tcgtgagcca taagaaaaat tcctttactc gtgccgccgc
      781 gcataatact acagcggatc atgttcattc ccggatacat tcaatcaact cctttcaact
      841 attcctattg gttaatctca ttatatacgc tagtcattca tttgaataat gaatgattta
      901 gcattataat atagataact tagatatttt atgatatata tccgccgtaa gaagggatca
      961 ttatgaacat cactgccatc cgcgtcttct tggaaatcgt caagacacac agtctttcga
     1021 aagcagcgga aaacctctac ttatcacagt cgacggtcag tcatcattta aaaaccatgg
     1081 aagccgaact gaacagtcag ctcattatcc gccatcaggg ccaacggacg atccacctga
     1141 ctcccaaagg cgaaaatttt cttcccctgg cagagcgttg gctctcctta tttaaggata
     1201 cacaggcgct tcaagacatg cggccgcaca gcaccttatc cctgggctgt acagacagtc
     1261 tcaataccta cgtctttccg gagctgtacc aaaatctcct ctcccatgaa gagacccttg
     1321 acttagacat ccgaacccag cattcacagg agatctacaa tctcctctta aatcatgaaa
     1381 tcgacacggg cttcgtcatt acctccttcg cttacaaaaa tgtcgtgacc cgaatcttat
     1441 tcaccgaacc gatggtctta ttgtgccaaa aagacatgcc gtttccttct gcttcgattc
     1501 atccccagga tttagacgta aaaaaagaaa tcttcatcga ctggagccct gaatttcgag
     1561 cctggcatga tttctggttc aataccgtca ccagtccgcg catcaagttc aataatccgt
     1621 cattgagcca ctattttcta aaaaataact actggtctat attcccgatc tccatggccc
     1681 gtttactgct gaaagaaatg gatttggccg tctatccact gacctcaccg ccgccagata
     1741 ggaccattta tcaaatcacc cataaggagc ccctttccaa tcacattccc gtactcacga
     1801 catttcaaaa atacgtcacc tacttccagg aaacgacgac gctatttcaa aaactctaaa
     1861 aggaatgccc ctccgctgtc gctgggccgg ccaaccaaaa acacccgata tcaaaggaat
     1921 ccctctgata tcgggtgttg tcatgtattc cgtttttgcg gtgcccctta atggaagcgc
     1981 caggtcatgc cgg
//
LOCUS       DAWDXZ010000344         1992 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10230_length_1992_cov_10.211668, whole genome shotgun
            sequence.
ACCESSION   DAWDXZ010000344 DAWDXZ010000000
VERSION     DAWDXZ010000344.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10230_length_1992_cov_10.211668"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          543 a          465 c          415 g          569 t
ORIGIN      
        1 tgtaggcacc ggcacttatg gggccgatca tccgtgcagt ttaacgttcg attttttgcc
       61 taccttaatt tggctgtttg gggccgggtt cggagatatg ttgtggaact ctcagacact
      121 tgctaacaag agcatcgttt cccccggaga cctcacagct gccttccaaa aatatccgtt
      181 cccttttgag gacggctatc aaagcggcgg cggggcttat ccctatgagt acgttttgga
      241 gtactatgcc gccttccaaa ataacacttt gcgctggtac gccaaataca cgcagaagag
      301 cgggaataac cagcccgtcg agatggacag cgccgcatat cagctcaatg ctgctgggac
      361 taactatgga tggatcgcat ttgggtaggt gttgtgtctt actagttgaa agcaatgtac
      421 acgtatgtca caccctgcaa attttcctga aacatcgcac ctgacgaaga gttgttacta
      481 taccaactaa attgcagttc tgctatgttg aacctcagat cagcctctgt atcagatttc
      541 gggccattga gggatacctc ctggcttatt tgaccatttt gccagaggaa acctgcacca
      601 ctatcactgc cccaaccgcc tcttgcctgt gtctgctgaa cgacaactaa cttaggcgaa
      661 aaatcgaacg attaaccaat cccgatatag aaatattttc gtccactctc gtttagctgt
      721 gcgccctgcc catcattagc agtgttccac catttaactg tattttcatc ccagttgata
      781 tgtgcgtagg ggaaattcat atatgacttg tcaattatag aaatcccact agtcatatcg
      841 cgggtccaaa ttgcagatac cgtaccctta ttgttacctg ttgtatcccg acattcaact
      901 gtgccaaaga gtagaacttt aggcgtaaaa tcgaacgatt atccgatggc taggtaatag
      961 taggttgttg cattcagttg cacagctgca tttggatcgc ctccatctgc ataccatgag
     1021 acctccttat cagtaaaagc aacacgatta taagctcggt acggaaacat aatatcgttt
     1081 ccattattgt aaaagggata gacaatttct tttgagataa ccataagggc tgaataattg
     1141 tcctgggctt cctgtatgat tactgctttg ggcacaaaat cgaaccttta tccgatggca
     1201 acataaaaat atgtagtgtt cagcccatta agttgagcgt aggagtctgc ttggaaatcc
     1261 cactttatag tgttagcgtc ttgtttaata ccgccatacg cttgaccgat tttcaagcta
     1321 tcttgtccag gacaccaaat gaaagaattt tgccagaatc cggcaacatt tagcggtatc
     1381 aagcccgtgc caatctcata gacaatcacg agcttaggcg aaaaatcgaa cgattaacca
     1441 atcccgatat agaaatattt tcgtccactc tcgtttagct gtgcgccctg cccatcatta
     1501 gcagtgttcc accatttaac tgtattttca tcccagttga tatgtgcgta ggggaaattc
     1561 atatatgact tgtcaattat agaaatccca ctagtcatat cgcgggtcca aattgcagat
     1621 accgtaccct tattgttacc tgttgtatcc cgacattcaa ctgtgccaaa gagtagaact
     1681 ttaggcgtaa aatcgaacga ttatccgatg gctaggtaat agtaggttgt tgcattcagt
     1741 tgcacagctg catttggatc gcctccatct gcataccatg agacctcctt atcagtaaaa
     1801 gcaacacgat tataagctcg gtacggaaac ataatatcgt ttccattatt gtaaaaggga
     1861 tagacaattt cttttgagat aaccataagg gctgaataat tgtcctgggc ttcctgtatg
     1921 attactgctt tgggcacaaa atcgaacgtt aaactgcacg gatgatcggc cccataagtg
     1981 ccggtgccta ca
//
LOCUS       DAWDXZ010000345         1991 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10234_length_1991_cov_8.304236, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000345 DAWDXZ010000000
VERSION     DAWDXZ010000345.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10234_length_1991_cov_8.304236"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          457 a          541 c          539 g          454 t
ORIGIN      
        1 aggcggatac cgtagacctt gggcagggtt ccggccgcct tcatttcctt gaagatcgta
       61 atggcatcgg gaacgccctg cttcaacgta ttatacgtat cggccaacaa aatgaggttg
      121 ttgggatact gttcggcata gtgacggaag gcttccagtt ccgacgagaa gctcatgacc
      181 cagctgtggg ccatggtccc taagacgggc atgccgaatc tggccccggc caggacgtta
      241 ctggtcccgt tgaaaccgcc gatcatggcc gaacgggcgc cgtagacacc ggccgatttg
      301 ccctgagcgc ggcgcaggcc gaattccata agattgtcat tgccggcaac agtccgaacg
      361 cggcgagctt tcgtggcaat gagactttca tggttcatga tcatggacat gcacgtttcc
      421 aagagcaagg cttcgtcctt cttggtctgg acgcgaagga ggacttcctg cggaaaggcg
      481 acggtgcctt cgggcatggc ataaatatcg ccggtaaagc gcagttcctt caaataatcg
      541 aggaattcct gacggaaaaa gccggtactc ttcaagtacg ccaaatcatc gtcgctgaag
      601 tggatgtttt ccacatagtc gatgaggtgt tccaagccgg cgacgaccgt atagcctccc
      661 ttaaaaggat tactgcgata atagcggtca aacacgcact gctgctcgtg cttgccttca
      721 aaaaataagc cctgcatcat cgtcaactga tacaggtctg tcaacaacgc tttcgtgtac
      781 aaaataactc gcctccaaaa agatgttata cctattatac atcagttagt ccaaaagaga
      841 taatcaatta ccgattttgc atgcttttca aaatgttata caaacttagc atgacagccc
      901 tttatgtatg ccggatatga aacaagctgc tcttccgatt catacaggat gagcagcttg
      961 gtcattattt tcttttcata accttgcggg cagcggcgat gacgtcttcg gccgataaat
     1021 gataggcctt caataaatca tcgggcttcc ccgattcgcc gaagcggtcc tgaatgccga
     1081 ccagttcgac gggaatggga tgatgctggg ccaaggcttc gcagatggca ccgcccaggc
     1141 ctcccatgat ctgatgttcc tcgcagacga cggctgcccc cgtttcagcc gcacaggtgc
     1201 agatcgtatc gacgtcgagg ggttttacga tgcggacgtt tacgacgcgg gccgaaatcc
     1261 cttccttttc aagggcttct gccgccacca aggcttcatg gaccatgagg ccgttggcaa
     1321 agagggtcac atcggttccg tcgcggaaaa cagccgcctt atagaggtca aaaggcgtgt
     1381 cttccgacgt aatgaccggt gccggattgc ggccgaagcg gacgtacgac gggccctgaa
     1441 tgtcatagac agccagtgtc gccttgtacg tttcccacca gtcgcagggc gtgatgaccg
     1501 tcatgttggg aatggtccgc atgagggcga tgtcctcgag agcctgatgg gtcgctccgt
     1561 cgggaccgac ggaaatgccg gcgtgagcgc cgccgagctt gacgtttaat ttattatagc
     1621 agaccgtgtt gcggatctgt ccccagccgc ggcccgtcaa aaagacgccg tacgtcgaca
     1681 caaaggggac gaagcccgtc aaagacaggc cggctgccgt gccgacgaga tcttgttccg
     1741 aaatgccgac attgatgtat ttatccggga attccttgcg aatccattcg gtccgcgtcg
     1801 acttggccac gtcggcgtcg aggaccatga cgtcatcgtg aaggcggcat aattctaaga
     1861 gggccttgcc atagccgtca cgcatcggta ctcggttcat gaccgcacct ccaattcttt
     1921 catagcctgc tcgtactggg cttcatcggg cgcagccccg tgccattcgg cctggttttc
     1981 catataagaa a
//
LOCUS       DAWDXZ010000346         1984 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10268_length_1984_cov_8.255054, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000346 DAWDXZ010000000
VERSION     DAWDXZ010000346.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10268_length_1984_cov_8.255054"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    395..404
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1216..1225
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          455 a          495 c          606 g          408 t
ORIGIN      
        1 ttcggcggat caaaaaagac ggatggaagc cacaaaaaaa acacgccctg cccaaaagga
       61 cagagcgtgc tgatagtata aaacaaggta tgaaccggag gctacatgtc tttcagccgt
      121 ccgacttcac tctttggtct caccttttta tggccaaagt tttcgacgat gatgaacatc
      181 atgggaatga ggaagacgcc gacgatggtc gcaacggacg taccgaagac gacggtgatc
      241 cccatgttga cacgggatgc ggcaccggca ccggaggcca tggccagcgg gatggagccg
      301 acaacgaagg ccagggacgt catgatgatc gggcgcagac ggatcttggc ggcttcaata
      361 gcagccgaca cgggatccat gccgcgctca tcgannnnnn nnnntattcg atgatcagga
      421 tggcgttctt ggcagccagg ccgatgacgg ccaggatacc gatctggaag tagatgttgt
      481 tctgctggct caggaggtag gcaaagacgg ctgccccaaa gacaccggcc ggcagactga
      541 atagtacggc aaagggaact ttccagcttt catagagggc tgccaagacg aggaagacga
      601 agaggatggc caaggcaaag acgtagaccg tcttgttgcc ggcttcgatt tcttcacggc
      661 tcatgccgct ccattcgtag gcatagcctt cgccgagggt atcggctgcg atttcttcca
      721 aggctttaat ggcgtcgccg gagctgtagc cattggccgg agccccttgg atggtaatag
      781 ccggatagtc attgaaacga gtgatgatcg atgccgatcc catcggtttc ggacgaacga
      841 aattcgatac cggaacgagc tggttgctcg agttgcggac atagatatgg ttgttggcat
      901 cgacggtttc acggaattcc ggagaagcct ggacgataac cttgaagtta cggccaaaga
      961 tggtgaagtc attgacctga taggaaccgt agaaggactg gagcgtcgaa tagaggtcgc
     1021 tcagggagac gccttcacgg gcgactttgt cgcggtcgat ttcgagctca tagcccggtg
     1081 tatccgcagc aaaagcggtg tagaccgaac cgatttccgg acgctgacgg gcagcggcga
     1141 tgactttctg tgccgtttct gacagttcgg ccgtcgtatg gccgccccgg tcttcgagct
     1201 gcatggtgaa gcctgnnnnn nnnnnccgtc gattggcggc gggtttaagg cgatgatcga
     1261 ggcttgcgga atctgggcgc cgcgggccat gattttaccc atcaaagcgt cgacggagag
     1321 atccttcgtc gtccgttcat cccagtcgtc aaggccgatg aaggcagcgg cggcgttcga
     1381 cttctggccg cctgccagaa ggccgtagcc gacgatattc atggccgtcg atacgccggg
     1441 ctgttctttg agccagttac ccatgtcttc actgattttc ttcgtttccg tctgagaaat
     1501 gccttccgga agggtgatat ccatcatgac gaaggcactg tcttcagccg gtacgaagcc
     1561 cgtcggcatg acccggaaga cgccgagggt agccgcactg atgacgatca ggaagacgac
     1621 gcaccagcgc aggtggagct gcaagtgagc caggatcttg ccatagcctg cgatgaggcg
     1681 gtcgaaccat tcattgaatt tattgaagaa tttgtaaatc cccttgggat tttcttcggg
     1741 cttatggata ttcaagatgg tcgcgcacat ggccggcgtc agggtcaggg cgacgaaggc
     1801 cgaaatgagg accgagacgg caatggtcaa ggcgaactgg cggtagagga taccgctcat
     1861 gccgcttagg aaagcgacag ggatgaagac cgaagccaag acgcaggcga cgccgataac
     1921 cgggttctgt acgttttcca tggcgatgat cgtcgcctct cgcggacttc gggcgttgta
     1981 ttta
//
LOCUS       DAWDXZ010000347         1981 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10280_length_1981_cov_9.566459, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000347 DAWDXZ010000000
VERSION     DAWDXZ010000347.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10280_length_1981_cov_9.566459"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          404 a          550 c          572 g          455 t
ORIGIN      
        1 aagcccgagg cgcggtatga aaatcacgtc gtcttgaagg aagaagacga tttgcgcgac
       61 cttgccgaca atgacaattt tgacctcatc ctcggcgact tgtactatcg ccgggccttg
      121 ccgaacttcc acggccaata cgtagatttt ccgcattttg ccgtatccgg gaggggataa
      181 gatgaggagc gaacggatct gtgtcttcgg catcgtccaa ggcgtcggct ttcggccttt
      241 tgtcagccgc attgccgatg ccaatgggat tttcggttcg gtctgcaata agggctctta
      301 tgtggaaatc attgctcaag gtgatgatgc gcagctggcc gcttttcatg aagacctgga
      361 aaagaaggcg cccgagcgct cgtcgatctt gaaggtcgtc acgacggctg tcgaggttcc
      421 ggcctatgat gatttttcta tcatcgagag cgccagggaa aaaggtgata ttttcgtgtc
      481 gccggacatt gccatctgca aggcttgcca gaaggagctc tttgacaaga acgaccgccg
      541 ctatctccat ccctttatca actgcacggc ctgcgggccg cggctgacga ttctcgactc
      601 catgccctat gacagggagc ggacgagtat gggggaattt cccatgtgcc ctcagtgcga
      661 gtacgaatac acccacgctg agacgcggcg ttacgatgcc cagcccgtct gctgcaacga
      721 ctgcgggccc gaagtttacc ttctcgaccg taaagaacgg ggccgggatg ccattacggc
      781 aatccgaaag gccatcgtcg acgggaaaat tgtcgctatc aagggcatcg gcggttttca
      841 cctctgctgc gacgctcgca atgaagaggc cgtcagcacc ctgcgccagc gtaaaggacg
      901 accggtgaag ccctttgccg tcatgatgaa ggacgtcgac gccgttcgcc gggaatgcct
      961 catttccgat ggggcagaag atgttctcga cggccatcag aaaccgatta tcttattggc
     1021 taaaagagac ggtctgacat tggcgccgtc cgttgcgccg ggcaatccgt ccattggcgt
     1081 catgctgccc tatacgccgc ttcatctctt gctgtttacc tatgacgacg gcttggaggt
     1141 ttccgacagc ttggtcatga ccagtgccaa tgagtcgggg gcgcccatct gccataacga
     1201 tgaagaagcg cggcaggaac tttccggcct tgccgatctc gtcttgtccc acaaccgacg
     1261 aatccgcctg cgggctgacg attcggtcat ggactggtac gacggcaagc cctatatgat
     1321 acgccgttcg cggggctatg cgccgttgcc cttcttttat gggaacgggc agggcaaaga
     1381 cgtcctggcc attggcggcg agctgaagaa ttgcttctgc atcggccgca ataatttatt
     1441 ctacccgtcg ccctatatcg gcgacatgag cgatataagg accgttcagg ccctgcgcga
     1501 ttcggttcag cgcatggcgt ctttattgga agcgtcgccg tcggttgtgg cctgcgatct
     1561 ccatccggcc tacaatacgg cggccgtggc ccgtgagttg ggccttcccg tcgtgactgt
     1621 ccaacatcac tacgcccaca tcctgtcctg catggccgaa aacgactgtg aacagcccgt
     1681 catcggcgtt tcctttgacg ggacgggtta cggcagcgac gggacgattt ggggcggtga
     1741 aatccttatc agcagccttc aagagtttga acgggccgct tccatcgagc ctttttggca
     1801 gcgcggtggt gacatatcgg ctaaagaggg ctggcgcatt gccgtcagtc tgatcgacgg
     1861 actgtggaag gatcgtcagc gtgtggcgtc tatcgtcgag actttaaaac tttgtgagtc
     1921 taacgaattg gcggctcagt ttttcctggc cgatcagcag atcaacagcg tcatctcgac
     1981 c
//
LOCUS       DAWDXZ010000348         1976 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10304_length_1976_cov_10.403436, whole genome shotgun
            sequence.
ACCESSION   DAWDXZ010000348 DAWDXZ010000000
VERSION     DAWDXZ010000348.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10304_length_1976_cov_10.403436"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    719..728
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          450 a          488 c          493 g          535 t
ORIGIN      
        1 cggttttaca ctcagtggaa cagatcgagg tcgactgtcc gaatcacgga agccgcatct
       61 ttaccgccgt ccttatcttg atttttgtcg ttctttttat cggtatcctt gctgtcatcg
      121 gtcttgtcgg attttaccag ttcgtcatct tgagacgctc catccgattc atcaccgatg
      181 atcgccttgg cttcattgat attgtggttc ttggcgagga aggcatcgcg tttttccggt
      241 gttacgactt taatcgttat ctggccgtcg cctttgacga tgtacggcgt agcgatggat
      301 gacttggcat cgctttgatt gtcgtcgccc acattgtgct tactcggctt aatgacatct
      361 ttagcactgg ttcccggttc tttaccacgg attttctcct tgctcacctt ggtcccgctt
      421 tcattgatca tgctgacgcc ctgatcgagg atttcaatga ccaaatcatt attggagtct
      481 tccttgtcga tttctttttt caaggcatca aaatctttgt aacgacgcag gcgtttgatg
      541 atttcatcgg tcaagttgta gcgctgcttt tcaatcaaat acggcaaagg aatgacaccg
      601 ccgccgcgga cttctaatac catatccccc aagggctgat ccttaggcac ggtaaagctc
      661 atggtcttca cttcatcgac accgcggtag ggatggagtt ttactttaaa gtatacgtnn
      721 nnnnnnnnta tacgttgtcc ccgggactta cgacaaccgg agcggctgta gcatcgacga
      781 tggaggccga cttgcgggcc tgggtaatat cgacattcat ggtgatatcg agaacgggat
      841 aatcaatgaa ttcgttatgc tttaagatat cgagaacatt gtacaattca tcaatgctct
      901 tttcattgat attttcctcg gaataataca tattgtggcg ggtaatgtcc ttatcgcggc
      961 cattagacga gcgaatggta taggacaagg tcgccgtgcc gccgccggtg cggtcgatgg
     1021 tcttattgac cgtattatag accgtcgtcg cagctaaaac aggagtcagt tcattgtctt
     1081 caatgatctt aacgcgttta gtcatggccg tatcggtatc ggtatcgcgt acgttgatcg
     1141 taaccggaat gcccggtgcc aaatagtcgt actgaccgct gatgccggcg ccccgatcct
     1201 gggtaatctt gccgacttca gcaccgatag atcctaattt gaaagagctg gacaggttgt
     1261 tgacaaccgt aaaaatatag gcattatgca tgaaataatt gatactgccc tttttcaaaa
     1321 aaggatgacc aaaggcgacg atgtgatcgt tgtcaacgta ggtaaccgta ccgatagcgc
     1381 ccatcttcat atcgccgtta acgaaggcgg cagccacact gctgccgggt tctaaaggca
     1441 tggcgacatc atcggtgctg gccgatgccg tatcgacggg catgaaatta taatcgggca
     1501 gcttgctctt catccactcg gccgacgtcg tatcaaaacc ggacatcatc aacggcgtag
     1561 caatgggaat gagcgacgat tcacgggcgt tgaaaggccg gatttcctcc ttggtcggca
     1621 cgttccacag tttcaacatg tcgttgatgg gcgtaatcat accgatgcgg gagttggtaa
     1681 aggaccagcc gtaggcaacg gcgccgagga gcttaccgtc gatgtaaacc ggactgccgc
     1741 tcatcccttg ggcaatgccg ccggtctgtt cgatgagggg accggaaaac ttggccagaa
     1801 tcaaatcccc cgacgggccg ctgttcttca tgacccctaa gatttcaacg gggaaggtct
     1861 ggatttccgt cccttgaacg acggtcttgg catagccggt cataccgatt tggacgtcac
     1921 tgacgttcat aaattcctga gcctgtgccg aaaccgccaa aagacaggaa aaggcc
//
LOCUS       DAWDXZ010000349         1971 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10334_length_1971_cov_7.204071, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000349 DAWDXZ010000000
VERSION     DAWDXZ010000349.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10334_length_1971_cov_7.204071"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    639..648
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          535 a          454 c          394 g          578 t
ORIGIN      
        1 ccgcttcatc gtatgaggca ttcggtaata acgaatggaa ccaaacggtt tttcattgat
       61 gttaaaaagc cggttagtac gcgaaaatgc ctggattaaa ttctgataaa ccaaaacctt
      121 atccaaataa aggacgttaa tccacttaga atcgaagccg gtcagcattt gattgacgac
      181 aatcagcaag tccaactgca tttcaggttc gactcgttca tagggcttct tgtgggccaa
      241 tcttgccgat acatcttttt taaacttagc gtatccgccg atatcgaagc actggccgaa
      301 ggttttatta taatcttcga gcatcgcttt tagtccgtct tctttatcaa gcgaacgttt
      361 tccaccttga ttatcaatcg tcggatcaaa aagcccggtt actttcagtt tgggatattt
      421 ttccctgaat ttacgatagt aacggacagc ttccggaatg ctgctggtag caaataaagc
      481 atgaaattta tgatttcttg acagggtcaa ccagttttcg gcgatattat cgactatcgc
      541 ccattcatat tcatcggtct gccaggcttc atctcctaca tagtcttcta tccccttcag
      601 gtattttccg tcctcctggc gttgtcctgc catgggcann nnnnnnnntt acagcctctt
      661 cctcagaact tgccccggat tcctgcaagg caactttctt tcgcaactcg ttgtctttat
      721 agaccattac catcgtgggg tcgaagccga gaacattttt atcacgaata ccatctgcaa
      781 tactatagcg atgcaattca tcaccaaaaa tatcagccgt cgtcgtcata actttttcat
      841 tttcacggaa tacaggcgtt cccgtaaatc cgaaaaagag cgcatggggg aatgtcgcct
      901 taatagtgga aagcatttcg ccaaaggtag aacgatgaca ttcatcaata ataaatacga
      961 gacgtttaga tttcatggcc tcaaggtctt ttgccttcat gctaacttct gcatcgtctt
     1021 tgatattgct catcttctga atggaagaaa caatcaaggt atttttagga tcattgcttt
     1081 tcatcttgct tatcagagtg accgtatctt ctgtttcctg cacgtcatcg gcattatcgg
     1141 caaaagcccg atattccttg agcgactgtg taccaagttc aatccgatcc atcaagaata
     1201 cgactttatc cgcatctttc gaagctgcga taagctgagc tgatttaaag gaagtcatcg
     1261 tcttgccaga tcccgttgta tgccaaatat aacccccaag ctgatttcct tcttcccagt
     1321 catgtttagc cacacggtca gaaatcctat tggcagcata atattggtag ctccgcatga
     1381 ctttcaaaac cccgtcagca tcatctgcaa cggtatagaa cccgactaac tgatgagcca
     1441 tgggaattga caaaagcagc tcggcaacac gcttccaatc gttgatagga tcattattca
     1501 aatctgcgca gtggaaataa aaatctggat tgaaccgtcc atcggggccc ggattcgaaa
     1561 aatacaaagt ctcggtcgga ttcattgcca caaaaatctg gataagagag aaaaagccag
     1621 taaaaagccc ttcatgagcg tatttttcaa tttgattggc cgcttcaatt acgggaacac
     1681 cggacttctt cagttcgata tgaaaaacag gcatgccgtt aatcagtaac atcaagtcac
     1741 cgcgtcggtc ttgcaatact ttttcgtgac gggcaaatcg aggttgttga acgatctggt
     1801 agcgactttg tccggcggca atttccatgc ggtcatagat tttcaagctt acttctttgc
     1861 catagtgcaa ggtatcttta gggttttttc gagttatgga tacagttttc ccattgatga
     1921 agccgtttaa agccaaagga gtccgaagac gattgatttg ttccagaaga t
//
LOCUS       DAWDXZ010000350         1969 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10347_length_1969_cov_6.508882, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000350 DAWDXZ010000000
VERSION     DAWDXZ010000350.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10347_length_1969_cov_6.508882"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1093..1102
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          559 a          471 c          456 g          473 t
ORIGIN      
        1 gtgatgacaa atccgttgat gaagaaaaga ctgaaaaaaa gaacgataca gaatccgacc
       61 ggaaagcata gcccctcatg agctatgctt tttcgttttc cgcaaggaac tcttttggct
      121 attgcataaa aagactgcct cttgtaatat ttttcacaaa atatgattgc agttaaagcc
      181 cgaaagcttt atactatata taaggttatc tcatactact attgcaaagt catgaaggag
      241 gagactttat aatgtacaag ctttcacaag ttccagaaga gtataagaag aaactgatta
      301 ctgctgaaca ggcagcggcc ctcgtacgag atggcgaacg cgtttcttat ggtctcggct
      361 gctcggctcc gtatgatatc gacattgaaa tcggtaaacg ggcaaaagaa ttgcacggcg
      421 tcgaaatcgt cgcttcgacc atcgtcaaag atgaacctta tgcggcatat ttaaacagtg
      481 actctaacga tcaaatccgc ttcgctacgg gccatatgaa cggctttgaa cgtaagatgt
      541 gcaaagacgg tcgctgctgg ttcctgccga tcttgtttaa cgaattgccg aagtactggc
      601 agcatatcga taaattgatc atccaggtcc atcccatgga taaatggggg aacttcaaca
      661 tcgggcctca ggctgctgac cttcgcggcc tcattcgcgc agctaagacg attatcctcg
      721 aagtcaacca gaacatgccg aaagctttgg gctatgaaac ggaactgaac ctctgcgacg
      781 tcgactatgt cgtcgaaggc tcgaaccacc agatgccgca gatcccgaat cgtccgggaa
      841 cggaactcga tgataagatc gcggaccaca tcattcccct catcgaagac ggcagcacgc
      901 tgcagctcgg catcggcggc attccgtcgg ctgtcggcaa gaagctggct gaatcggacg
      961 tcaaagattt gagcggtcat acggaaatgc ttgtcgactc ctatgtagac ctttatgaag
     1021 ccggtaagat tacgggcaag aagaaccgtg ataagggtaa aatcatgtac gctttcgccg
     1081 gcggtacaca gcnnnnnnnn nnttcatcga tgacaaccag atcgtattca acgcaccggt
     1141 aagctacatc aacaacatcg gcgtcgttgc cagcatcgat aaatttatct ccatcaacgg
     1201 ctgcatcgac cttgacctct atggccaggt caacgctgaa acggcaggct accagcagat
     1261 cagcggtacc ggcggccagc tcgactttgc acagggcgct ttcgcatcgg aaggcggcaa
     1321 gagcttcatc tgcgtccatt cgacgcgtaa atttaaagac ggtcacgtcg aatccctgat
     1381 taaaccgacc ctgccccagg gcacggtcat cacgacgccg cgctcggctg tccactacat
     1441 cgttaccgaa tacggcacgg ccctcttaaa aggcaaatcg acgtgggaac gtgcagaagc
     1501 actgattaac gtcgctcatc cggatttccg tgaagatctg attaaacagg ctgaaaaaat
     1561 gggtatctgg acacagacca gtaaagttac catgtaatga attttaacta agcccaaaag
     1621 gctcacacct cggtgtgagc ctttttttcg tggaatctta atgatttttt atagaagggt
     1681 ttctaacgtc cgatttaaaa tttttgacat aaaatcaggc aagtcgtaat caccgtcttg
     1741 agcagaccag tcaaacatac agcccttggc aatgacgcat aaatctttgg acaccatata
     1801 aggagttcgt cccggggaga gttgaaatcc gtcagcaatt gcattttcta aatagtattc
     1861 ggtgatttta cggacctgat ttgcaaaaat ttcagtattc ataatatcat ttgtgggcga
     1921 tataaaatgt tttgtgaaat cggcccccag gtcagctgta aattggcac
//
LOCUS       DAWDXZ010000351         1969 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10345_length_1969_cov_9.961860, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000351 DAWDXZ010000000
VERSION     DAWDXZ010000351.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10345_length_1969_cov_9.961860"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    799..808
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1528..1537
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          492 a          490 c          532 g          435 t
ORIGIN      
        1 tgcgttgccg aatctctgga agacctgaag aagaacttca cggacggctg catcctggtt
       61 ctccgcgccg ctcgtgctga ctttgtagaa tatatgaaac gcgccagcgc tatcgtatct
      121 gaagaagcag gccttaccag tgaatcggcc atcgttgcca tcactctcgg cattccgacc
      181 gtcgtcggtg cagaacatgc tgcggatacc ttgaataacg gcgaagtcgt caccgttgac
      241 gccagccgcg gcacgatttt ccgaggcgca gcgaacgctc gataaatcat aaagaatact
      301 tcgtgaatga ttaaaaactc cctccgtgag tctgccttat aaggcctgat ttatggaggg
      361 agttttgcgt taaagactgt ttttgggttt aatttcgtga ttgttatgtc actaccggtg
      421 aggaaatggg ctaaggatat tgttttggga cgtgaaaaaa attgccatat cttcactgcg
      481 gctgctttta tattgtgact gtattgtgca cgggccatta cttcggtgtt atcgacgagg
      541 tatataatcg ggtaaaacaa gaaaggatga tggttatgaa aatgacgaag aatatggcca
      601 aactggcagc tgctgtctgc ctgtctatgt ctttaggatt tggtgtcgta tcggccttgg
      661 atgtaccgtt ttcggacggg gcgatgccgc agggcatgcc tccacaaggg atgccgccgc
      721 agggcatgcc tccacaaggg atgccgccac aggggatgcc gccccaaggc atgccgcctc
      781 agggacagca gggaaaatnn nnnnnnnnct gcgcagcgga ttacgggcaa agaggcgctt
      841 ctggtcaatc aggatatcac ggctgatgag gccgatgaaa attccgtcct ggcagaacaa
      901 ggcgctgacg tgaccctgaa gcaggttcgc ctttccaaga cgggacatac gaccagtgaa
      961 gatggcagta attttaacgg tcagaatgcc gtcctcctcg tttcgaacag tacggcgtcc
     1021 cttaccgact ctacggtcac ttctgatgcc gaaggggcca atgccgtctt tgcgacggga
     1081 aaaaatgcag tcgtcactgt taaagacatt aagattaata ccaaaggcaa ttcgtcccgc
     1141 ggcctcgacg cgacgtacgg cggtacgatt cacggtgaaa acgtagatat tacgacggca
     1201 ggggctcact gcgcggcctt ggcgaccgac cggggtgaag gcaatgtcta cgccacgggc
     1261 agtacccttt cgacgagcgg ggaaggcagt ccggtcatct attcgacggg caacatcgtt
     1321 ctcacgaaga gccatggtgt cgccaaaggc agtgaaatcg cctgcgtcga aggaaagaat
     1381 tccatcttca tagaagattc gaccctcacc ggatataaga accacggtgt catgctctac
     1441 cagagctttt ccggcgatgc cgggacgggg acagcttctt ttacggctaa aaattcgacg
     1501 cttcgcaact attctgacgg agctatgnnn nnnnnnnagc tatgttctac atcaccaata
     1561 cgaaggccgt tgccagcctg accaatacgg tcattgaatc gccgaagaac aagaatctca
     1621 tcgaagtcgc cagcgaccgc tggggaacgg aaggttcaaa cggcggtgac tttgaattta
     1681 cggcagccaa acaaactctc agcggtgacg tcatcgccaa taaaatcagt actgtttccg
     1741 tcagcttgac agacggctcg aagtggagcg gggctatgaa tccaaagcac acggctaaat
     1801 cggccagcct ttctctcgat gcctcgtcga tttggaacgt gaccggcgac tcctacgtcg
     1861 cagctttgac ggacgaggat acgacacttg gcaacattca cagcaacggc tacacgattt
     1921 attacgataa gagcaacaag gccaatgcct ggcttaaggg acagacggt
//
LOCUS       DAWDXZ010000352         1964 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10371_length_1964_cov_8.678889, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000352 DAWDXZ010000000
VERSION     DAWDXZ010000352.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10371_length_1964_cov_8.678889"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          558 a          427 c          559 g          420 t
ORIGIN      
        1 tgtgaaattg aaaaaagtgc ttttggccct cggtttttcc ctggccttgt cggcctcggt
       61 ctttgcggcc gacaatatgt ggcagtataa tcagtataca tcgattgaca tgaattcggc
      121 gaaactctac tacgatatgg gccgccaggt tctgtccttc aatactgtgg aaaaatccgg
      181 cgatacgacg gattactgta cctatgtata caacatcgat gatcagacga ttcagctgaa
      241 gaagatgatt actcagacta agaagcataa gtataagagc agtttctatc ccgaagcgat
      301 caaagacggt aacgctgtta tcaaagccag agcccgtatg gccacggacg tgttggaaaa
      361 ggtcatggaa aagcagaatg ctaaaaagta agcccttggc ggcgaacggg ctgccgaagt
      421 gtgcctcatc tgccttacgt tctttccgcg tatagtcgga agagctgggt ttccggtgct
      481 gtcggcaagg gagcgcaatc aaaaagggac tgctgcatgg agcggttttc ttcatgcagc
      541 agtccctctt ttgttatttc cctttgacgc agatgctgag ccatttcgga agcttgtgct
      601 cgacgacgac ccaggaatag gccgacgcac aggcgacgac aatgatgtaa atggcgatga
      661 tgtgaatggc tcgcaggtaa atatgcaggt gggccgcaag gcccgtgcag aggctgagaa
      721 aaacaggatg gaccagatag atggggtagg aatagtctcc taaccgggag aagaactgat
      781 ggacggcgtg cggtacaggc cggcattccc accagtagag gagaaatagc atcgtcgaca
      841 gggtataaac catgccgata gggctgagct ggtgaatggt gaagactgcc gataaggggt
      901 catagccgag gtaatgcatg acgccgtagt aggcggccag catgcccgcc gttgaagcga
      961 tttgaaggag attgatcacc gtgccgtgcc gtttgagcca ggcggtgacc gacgaaaagt
     1021 gttccgccgt aaaggcgccg aacatgaaga tgaataaata gtgcaggacc atgtagttca
     1081 ggcgcagatt cgaggcatct tcgaggaagg gattgtccga atggagattc caactgtaac
     1141 tcgagtaaaa attaaagatc atgttgacga gaaacagggc tgtaaaggaa aggaccggtt
     1201 tgcggtccat gaggtgcagt aaaggccgcc acagaggcat gaagaggtag aaccataaga
     1261 ggatgaccag gaagtagata tgatacatgg ccaggccata ccacaaggtg cgaaagatgg
     1321 catagggatg aaagagattg agattatggc tgatagccga tacgaaggcc atgtagaaaa
     1381 acgaccaggc caggtagggg acaaaaactg tccgcagccg gcgccgcaaa tagtctttat
     1441 agtcaaaggg ctcgtggagt ccctgactgt aaaacatgcc aaaggccgat agaaagaaaa
     1501 aggccggcac ggagaatcgg gataagattt ctaaaatggc gacgagcgct aaatctggcg
     1561 acggattgac gatggctact gagccgacat tgatggcgat gacgccgagc atgcaaagtc
     1621 cgcgcatata attgatagaa tgaataaatc gttttgacat ctgacacctc ggaagataca
     1681 atatagatac taaagctata ttctattata acaaattttc gcaaaatgga cagggaggct
     1741 gtatgaccga cgacacacaa aaggtaaccc gaacgggact gatactggca gcgaccttga
     1801 ttttacaagg gctcaggctc atcatcccta gaccgcctca gatcagtctg ttcctcatcg
     1861 ggtctttagt caatgcctgc ctgattacgg ccgtccttac cattgatcgg cgggccggtt
     1921 ttgtcgtcgc cgcctgcacg cctgtttttg cctggctcga aggt
//
LOCUS       DAWDXZ010000353         1961 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10388_length_1961_cov_9.480063, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000353 DAWDXZ010000000
VERSION     DAWDXZ010000353.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10388_length_1961_cov_9.480063"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1151..1160
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1685..1694
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          484 a          508 c          504 g          445 t
ORIGIN      
        1 gctgtcgtca ttgaagcgga tggtaaggat gaatgggttg aaggacttcc cgaagctcac
       61 gactggaaac agctcgacag caaagaaatc ctggaactca tcaaaaaagc tggcgtcgtc
      121 ggctgcggcg gtgctacctt cccggctcac gtcaaactgg ctccgaacaa accggttgat
      181 actttcatcg tcaatggcgc cgaatgcgaa ccgtacctga cctgtgacta ccgtgcgatg
      241 gtagaaaagg aaacgacgga caaactcgta acaggtgtcc agatctgcat gaaagtgctc
      301 ggaattaaaa cggctttcat cggaatcgaa gacaacaaac cggaagccat ccagaacctg
      361 accgaagctt tcaaagatat tccctaagtt acggttgttg cctgcaagac caagtatcct
      421 cagggtgctg aaaaaatgat gatcgaagcc tgcacgggcc gtcaggtaga accgggcgga
      481 ctcccgatga acgtcggctg cgtcgtcagc aacgtcggca ccgtcatcgc tatcaccgat
      541 gctgtctgtc agggtattcc cttcatcgaa cgccttacga ccgttacggg tgactgcatc
      601 aaagaaccga aaaacctccg tcttcgcatt ggtactacct tccaggaagt tatcgactac
      661 tgcggcggct ttaccgaaaa accggatcgc ctgattgccg gcggcccgat gatggggctt
      721 gcccagtatc agctcgatgt accgattacc aaaggtgcgt ccggtatcct ggcactctct
      781 ccggaaaaat gccaggccgg cgaagaatct ccgtgtatcc gctgcggccg ctgcgtagaa
      841 gcatgtccga tgggcctcgt tccgagtcag ctcagcatct tctcgtcctg ccaggcttgg
      901 gataaatgca tggaatacgg cgtcatgaac tgtgttgaat gcggcagctg cgtttatacc
      961 tgcccggcaa aacgcaatat tgtccagtat atccgcaatg caaaaggtca gtgcaaagca
     1021 atgcagcgcg aagcgcaggc gaaagcagaa gcacagaaag ccaataacta attggaagaa
     1081 gggatatcat gagtccagaa gctaaaggac aaacaaatgg aaaggatgct aaagacatgc
     1141 aggaaatcaa nnnnnnnnnn tcgtcgtctg taaacaagct atgggcggcc tcggctacaa
     1201 tattttcaac ccggctcatg tcggtcgtgc cggcctgatg gtttcctggc cggtggctat
     1261 gaccacctgg acacaggtca acggtacggt catcgatggc gttgccggtg caactccttt
     1321 gaacgttttc aaacacggcg gttccgatgc cctgttccag cttttcggta cgaatgactg
     1381 gagcagcatt taccagagcc tgttcttcgg tttccgtaac ggttccctcg gtgaaacctc
     1441 tacggttctc ctcatcctcg gcggcttatg gctgatttac aaaggttatg ttaactggca
     1501 ggttccggtc gtcatgatcg ccaccgtcgg tatcctcacc ggtgctgtat acggcagtgc
     1561 caacgttgcc ctcttccaga tgatggcagg cggccttatc attggtgctt tcttcatggc
     1621 aaccgatatg gtcacggctc cgattaccgt taaaggtcag gtcatcttcg cattaggctg
     1681 cggcnnnnnn nnnncatgaa taccttgact ccgctcatcg atcgtttggt taaaccgact
     1741 gtctttggtg cggtaaagac aaagggggct aaataaaatg gcagacaaaa acgaaagcgg
     1801 gatctttaag gtcgcaatga accttatcct tacctgcctt gtttccggtt gcattatcgg
     1861 tgtcgttttc ttcatcaccg gcccgattgc tacggcaaaa gcagaacaga tgaaacagga
     1921 ttcgatgaag accctcgttc ccgatgctga caaattcgtt c
//
LOCUS       DAWDXZ010000354         1951 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10436_length_1951_cov_8.048523, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000354 DAWDXZ010000000
VERSION     DAWDXZ010000354.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10436_length_1951_cov_8.048523"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          391 a          503 c          563 g          494 t
ORIGIN      
        1 tgcccgtata gacggaatgc ttcttgcccg acagttcgcg cagcatttcc atggccgccg
       61 tcccgttttt cggcttgccc atcatgacgc cgtcgagggc gacgatggta tcggcgccga
      121 ggacccactg gcccgggaac ttttgggcga cgtcgcgggc cttgccgagg gcctggtcct
      181 ggacgtactt ctgaggatct ttcttcttcg gtgattcttc atgataagaa ctcgggttga
      241 ccatgtaggc cacgccgatc tgggtcaaca gttcgcggcg gcgcggcgaa ccagaggcta
      301 aaataagcat gtaatccctc ctgtaagtta ctgaatatgg cgaaagagca caccggctaa
      361 gagaatgccg atgacactca gtaaattcgg tgccagatgg atgccaaact gcaattccat
      421 gagataaaga ttgaggtgaa tctgctggat gttcaagatt tcataatgct gtgtcaatgc
      481 cggcaaaacg ccctgcaggg ccgggacggc gtataaggct tcgcctacga taccgccgag
      541 gatgccgccg gcaatgagaa agataaataa ggacagccaa ctatgcgagc caagattttt
      601 catgcttccc ctccattata tacaccatgc gaagaaaggg ctgccccctc tcaggaaatt
      661 ctaatccttc cggagaaaga caacccttta tcatcggcgt tattgatttt tctgtttctg
      721 catcttagcc cacgtgtcgc ggaggccgac gatgcggttg acgaccatgt gatctgccgt
      781 cgtatcggga tcgatgacga aataaccctg gcgcaggaac tggaacttgt cgccggcctt
      841 atagtcagcc agggcctttt cggccttact gtggtagacg acgaggctgt cgggattgat
      901 ctggtcgagg aaatccttgg aatcgccgtc atcctggcga aggagggaat cgtagaccct
      961 ggtttcgacg tcgacggcat cttcggcatt gacccaatgg agggtccctt ttaccttgcg
     1021 gtcggcgttg gcaccgctgc cgctcttact ttcagggtcg taggtgcagc gcagttcgac
     1081 gatgttgcca ttttcgtctt tgacgacttc ttcgcatttg atgatgtagg catatttcaa
     1141 gcgaacttcc ttgcccggag ccaggcggaa gaatttcttg accggttctt ccatgaagtc
     1201 gccccgttcg atgtataaat gcttgctgaa ggtcatgtcg cgcgtgccca tggcttcatt
     1261 ttcctggttg ttgtcggcct tcatcgtttc cgtctggccg tcgggatagt tggtcaggac
     1321 gactttgatc gggtcgatga cggcgccttc attttgaggt cttcgcggat gcagtattcg
     1381 aggagggcca tttcgaccat gttgtcggct ttggcgacgc cgatgcgttc acagaagtcg
     1441 cggatggctt ccggcgtata gccccggcgg cgaatgccgg aaatggtcgg catacgggga
     1501 tcgtcccagc cgctgacgat gcccttttcg acgagggtcc gcagtttgcg cttactggta
     1561 atcatattgg tgacgttgag gcgggcaaat tcgatctgcc gaggcttctg tccttccgga
     1621 tcttcccagt cttcgaggac ccagttatag agcggacgat gggcttcaaa ttcgagggta
     1681 cagatggaat gggtgatccg ttcaatggcg tccgatacgg gatgagccag gtcgtacata
     1741 ggatagatgc accacttgct gcccgtccga tgatggtcgg cgtggacgat gcggtagaga
     1801 atggggtcgc gcatattgag gttggggctg gccatatcga tcttagcacg gaggaccttt
     1861 tcaccgtcgg cgtacttgcc gtccttcatt tcttcaaaga gcttcaagtt ttcttcgacg
     1921 ctgcggttac gatacggact ttccttcccc g
//
LOCUS       DAWDXZ010000355         1951 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10437_length_1951_cov_7.119726, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000355 DAWDXZ010000000
VERSION     DAWDXZ010000355.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10437_length_1951_cov_7.119726"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          484 a          571 c          483 g          413 t
ORIGIN      
        1 ggcaggtcag tatggtaaag aaggggtgct ggctttattg gaacagcatc cggaaatcaa
       61 attcgtatcc ctcgtaggca tcgatttggc aggcaatgat acggacgaaa agattcctat
      121 cagcgttttc tttgatgact acgataaatt cttcaatgcg acggcattcc agaccgatgg
      181 ttcgtccgtc gtcctgccgg gcatcgccat cttgtccaat gcccgcgtcg acatcaaagc
      241 cgacccgtcg gtcagctggt tcgtcgacta caacgacgac aatatcgacc cggaaacggg
      301 aaaacccgta gggactctgc gtattcccag cttccttcgt catgccacgg gctacatcga
      361 ctcccggtcg atcctccgca acacctgcga ctacgtcagt gaccaggtct tggcgctggt
      421 caaacagtat ggcctgcccg gtacgtcggt caatgtcgat gacatcgaaa aaatcgtcct
      481 cacgacggcg acggaactgg aattctgggt caagacgccg agccaggatg tagcgacgcg
      541 cctcttgtcg gcttcgcaga aattgcagga acagtattgg cagcgcacgc acggcgtcgt
      601 tcgtacggct cttgaacaga ccatcgaacg cctcgaaaaa tacggtctcg aaccggaaat
      661 gggccataag gaagtcggcg gcgtcaaagc ccagatcgac gattccggta aattgacctt
      721 cgtcctcgaa cagctcgaag tcgactggaa atttgccaac gacccggtcc agacggcaga
      781 taatgaaatc gcagcccgta tcatcgtccg tgaagtattc cgtgaaaacg gccttgaagt
      841 caccttccag gcaaaaccga ttcacggcgt tgccggttcc ggtgaacata cacacttcgg
      901 catcggggcc atcatgaaga acggcaaatt cgtcaacctc ttcaacccgt ctgatatgaa
      961 gagcgacttc ctgtccgtcg tcggttacgg cgccatcatg ggcatgctca aacactacga
     1021 agtcatgaac cccttcgttt cggcaacgac agactccctg aaccgcttga aaccgggttt
     1081 tgaagcccct gtttgcatcg taacggccct cggcggtgat cgtccggacg tccagacccg
     1141 taaccgttcg gtcctcatcg gcctcatccg caacatcgca agcccggcgg ctacacggtt
     1201 cgaactccgt tctccgaacc ccttcaccaa tacctacatc gccatcgcca tgggatattt
     1261 ggccgccctc gacggcatca aatatgctct ctccagcggt aagaacggcg aagccctcct
     1321 ggctgaactg tccaagaagc ccggcgaaga tgcagattat cttgaaaaag accgggctta
     1381 tcgctcggaa gtcgacgtct ttgaaaactt caccagtgaa gaacgggatg ccatgttcgg
     1441 caaggcaccg cggacggtct gggaaaactt caaggccctc gacctccatc cggataaagt
     1501 agataccctg gcctgtgacg ctttccagaa gcgcatcatg gaatcgttcc gcctcggcgc
     1561 cctcaaccgc tgggccctcg aactccagta ccgcctcatt ccggaaaacc tctccaaggt
     1621 catcgctgcc aaacagctcc atgccgacgg cgacagcaat gcccttgaag tcgaacgctg
     1681 gaccaaaatc aacgccctgc gtacggaact gggccgcgat acggaaaaga gcctgtccat
     1741 tttctcgaag atcaaagaag ccctggctgc caaagactac gatacggcat ccaatctcat
     1801 ggtcgaaatg agtgataaga tggacctctt gaccgatatg tactacgaat attcgtataa
     1861 cgctttctaa acccagttga taaagtttta gctatataga aatgagctgc tccgcacatg
     1921 ggggcagctc atttttgcta taatatatgt t
//
LOCUS       DAWDXZ010000356         1947 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10462_length_1947_cov_6.114165, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000356 DAWDXZ010000000
VERSION     DAWDXZ010000356.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10462_length_1947_cov_6.114165"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    496..505
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          623 a          341 c          452 g          521 t
ORIGIN      
        1 tgcctatgga ttccgcaata cacagggcct cgcctttgtt ggcgataagc tctttgcgac
       61 agagcatggc ccgtctacgg atgatgagct gaacctcatc gaaaaaggcg gaaactatgg
      121 ctggccttat gtggcaggct atcgggataa tgaatcctat gtctatgcca actattccaa
      181 ggctcccaaa gaattacagg aaaaattcga tcccaacaac atccccgcag gcgttcccac
      241 tcagaaggaa acagactttg atgctccgaa tctcaaagat ccgctcaaat cctttaacgt
      301 cgtccgcaat ggatataatt ttgccgatga cacctatgca ccgctttact atataggctg
      361 gccgaccatc gcgccttcca gcgtggccta ctatccggac aacgggaaaa ttaaagaatg
      421 gcggaattcc ctgctcatta cgacccttaa gagcggcgcc atctatcggg tgaaactcga
      481 cggcaagaaa gaacannnnn nnnnnggtcc agggcgatgt agaaaaaatg ttcaagacca
      541 ataaccgtta ccgcaacgtc ctcgtaagcc cggatacgac aaaaatctac gtggctacgg
      601 ataacggagg caatgcgatg gggaaagatg gcaagcccat taccgaaatg gaaaataagg
      661 gagccatcat cgtcatcgaa tacacgggag ataagaagga caaataaaga gacgaactcc
      721 cggcaggaat tttatgtttg gtctctcgtc aaaaataagg catacatgaa tctatgagaa
      781 aatggctcat cgtcataacg acggtgggcc tttttctgta ttgttttctg atgggaactg
      841 tcacatattt tttgtaatgc ctgaatctcg acgtacgtaa caccagtcaa gagatttatc
      901 cacatcaaat ccgtagtggt gattcatacc cctgtacgat tcttaacgat tcgggtacaa
      961 gaaatatcaa agctttgaaa aagagagtgt atgagaaaag ccggtacagg catctgtcta
     1021 ggatgttttg tatcggcttt ttgttgtatt cagatcgata aagatgaact tactcaagtc
     1081 tttctgtttt ttatatggaa agaataaggg cgtaataagg tgatttgcca atcggatgca
     1141 atgctcttaa atggccttat actgcgatgt aaggacgttt tgttgttctc tttttcgacg
     1201 aggtgtaacg cgttacagaa agagaagata agaagagata agaaaagaag ggagataaga
     1261 tatagaacag acaagataca agacaagata taagaaaaga tgaaagggcg atgagaacgc
     1321 ataagggaaa catgatattt ttttaatttt ataaattttt gcacccctga ttttgagtca
     1381 ggcaggggag aaaatcgggt aagatatttc ctttatccga ttcgatgggt ttgtctcttt
     1441 ttgccggaag ttgtatgttc agtgtggaca aatgtattaa tttgcaaatt tttgaaagtt
     1501 cagaaaacgt taagtatatg aatgtcagcc ttagcattta gcatgtattg agtcagaaat
     1561 ggatataatc aactgaaata agggaaaata cgaaaaatga aaatgagaaa atggggcctt
     1621 tttggaaaaa gtttgaaaaa aactttagac gattggcatt ttttttgtat tagtatagta
     1681 gaggggaaat ttcaaatggc ctgtctaagg tgatacagga tgtgccggaa atgaaagggt
     1741 acgatgacag aaaacgaagg tttggaacgg gggacggtct tgccggattt tttggctgaa
     1801 actgtgtgtg tagaatggac gaatgtatta atttgtaaat ttctaaaagt tcagaaaact
     1861 ttaggtgttt cgtagaaaac tccttatagt caagaataaa ttcatgggaa aacaatctct
     1921 atttttgaga atccatgtat tttcgag
//
LOCUS       DAWDXZ010000357         1943 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10486_length_1943_cov_5.420021, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000357 DAWDXZ010000000
VERSION     DAWDXZ010000357.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10486_length_1943_cov_5.420021"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1040..1049
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1612..1621
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          358 a          531 c          567 g          467 t
ORIGIN      
        1 atgtcgccgc cgtggaagac agacgtcacc tggtgttcga cagacacgtt gccgtcgagg
       61 cccagtttcc ggcagacccg gtcgcggagg aggtccatat cgcccggcgc ataggccgac
      121 tcggtcttga ccttccacca atggctttcc aggtcagctg caatggaggc gacggcgacg
      181 gtctggccgc cgtcgacggg aatcaatata tcatttttag gaacaatatg ccaaactttc
      241 atcgattatc ctttctttca ggcacgatgg caggctactc ttcgtccgaa tagtcctctt
      301 catcgtaagc gtcttcgtca tcgccggcag gagctaccgt gacgttgaat tccatcatgt
      361 cgtggggcgc attgaggagc ggatcctggg gctcgagatc ggccgtgctg acgacttctt
      421 cactgtctcc gtcaggcgtc aaaacgatgg cgtcattggg atcgtaatgc gacgtgatga
      481 ggtcgatatg acggtcgccg tcgctgtagg tcacggtccg gcggatggtg atgtcgtggc
      541 cgtcgtagcc aaactgcttc agcgtatcgt cagtgacgct gtcatcgtgc ttctttatga
      601 tcttgtgcgg taaattctgg agatgggtcg tttcaaaggt gaccgtgctg gtatcggcgt
      661 ggttgcctaa gatatacgtc gtcaccgtgt tggccccgac gacggtatag acggcgacgg
      721 ggtgctggaa ggggttggta aaggcaaaat caaggccgtc atcggcaacg gtagcatcca
      781 tgccgaccgg tacgtaggct gccggagcga aatgcggttc ccggctggcg atgaagaggc
      841 ccgcccgaag ggctgcgttg aacaaggtcg tgctgacctg gcagacgccg ccgccgacgg
      901 attgttccag tctgttttca aaataggacg gggcctgttt gtagccctta tctgccgttc
      961 gcgtgccgac gtatttattg aatgaaaagt cctgttggct ggcgacgaag gcatgattca
     1021 gccgttcctg ggccaatttn nnnnnnnnnt ggaatccttg aggcctgccg tattgatgac
     1081 gatgcggtct ttttccggtt cgacggcgac ggtcttattg tcttccttga cataggcaaa
     1141 ggcattttcc ggatccttgt cgtgtttgtc gtggagatcg ctcagggcct tgtcgagttt
     1201 ttcttcatcg acggcaatgg ccaaggggat gtccttgcca tacatcaagg tcgtcgccac
     1261 gtcagccacg cgctcccaag gcatgccctg ccgtccgatg aggtggagtt catcgtcgat
     1321 gtactggcgg tcgagcttgg ccccgaggtc atcgaggttc agcgtcgtat cgacgccgtc
     1381 cgctgccaga tggagcttgc gcccttcgat atctttggca tggatgtcga ggtaattggc
     1441 aatatcttca tcggtcatgc cctggactga atgaccgtcg agggtcacca tctgttcggc
     1501 cctcgtctgg gcggcaaaga atccgccgaa acccaagatg cacagggctg cgacgacacc
     1561 gcctattttt ttccatgacc gagacttcgg agccggcagc ccatgttttt cnnnnnnnnn
     1621 nttaaagaaa gcgcttcccg tatcgacgtt gcccttgctg taaaacaaga ggctgttgcc
     1681 gtcgtcgagg cgtcccgctt cgtcgaggaa ccactgttct tctttgctca ctttgctccg
     1741 cgaccggccg ccccgcttca agggcagaaa gggacagtcg taccagacgg tgatcgtgcc
     1801 gctgctaaag aggctcgtcc cctgaaagga ttcgacgacc gttgccgccg ccgcatcttt
     1861 ttgaaaggtc tgcacttcag cgtcgccgcc ggaccgtttc cgggccgctt caaaaaactg
     1921 tctccgcccc tcttccatga ggt
//
LOCUS       DAWDXZ010000358         1943 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10483_length_1943_cov_6.784958, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000358 DAWDXZ010000000
VERSION     DAWDXZ010000358.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10483_length_1943_cov_6.784958"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    373..382
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1310..1319
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          452 a          528 c          499 g          444 t
ORIGIN      
        1 aaggccggac tgccgtcgtc attgctcatc gtctgagcac ggtccgcaac gccgacaaca
       61 tcgtcgtcat cgaccatggt cacatcgtgg aaacggggac tcacgccgaa ttattggcca
      121 aagacggcct ttatgcacac ctctacgccg ttcaatttaa cgataagaaa gaagcttcag
      181 aaggttgata gcgatgaaga atttaaaatc ccatccgatg tactggctgt ataatattct
      241 cctcctcatt tattgggcga ctctcattcc catgttgatt taccgcttaa tccatgaaga
      301 aggtttttat cagcgcatca aacagagcat cggctttttg ccggatgaat taaaagaaaa
      361 aatatccaat cgnnnnnnnn nngccatgcc atttgggtcc atgccgcctc ggtcggagaa
      421 atcgtagcgg ccagccccat tgtgcgtgaa atgcgaaaaa aacaccccga tgaagtcatc
      481 atcgtatcgg tcgtaacggc gacggggttc cgcatggccc atcaaatcat aaaaggggcc
      541 gacgggatcc tctacttccc tttggatttg ccttatttga ccaaccgtat cctgaccatc
      601 gtcaatccgc aggctatcgt cttagtcgaa acggaaatct ggcctaactt tttgcgcatt
      661 gctgctgaca aaaagattcc ggtcatgatg atgaacggcc gtatcagtca gcgcagtgcc
      721 gttcgctacc gcctgattac cttttttacg cgccgcgtct tgtcgacgat tcgcatgttc
      781 tgcatgcaga gctatatcga tgcccagtac atcatcgata tcggcgccga tcccaataag
      841 gtcatcgtca cgggcaacac caagtacgac cagacttatg gtatcgtaac ggaagaagag
      901 aagaagcgct tccttcatga actcggcttt tctgaaggca gtcccatcat gattgccggc
      961 agtacccacc gcggcgaaaa cagtccggta tataaggcct ttgtgaaaat tcgcgaccat
     1021 tttcccgatg ccaaattgat tattgcaccg cgctatattt accaggccga cctcatcgtc
     1081 gatgaaggca ttaagcacgg cgtgaccatg gtcaaacgca gtgacatgat ggccggaaag
     1141 gccgttcgct cggcctatga cggcgtcgtc ctcgatacca tcggggaact ggggcgggtc
     1201 tacagccttg gcgatttaat cttcgtcggc ggcagtttgg cccatattgg aggccataat
     1261 atcctcgaac cggcggcgca cgggaaaccg atcgtcgtcg gaccgaatan nnnnnnnnnc
     1321 ctatgtcctt gagaacggca ggaactgctt actttcatta tttgacagga agcaaagaac
     1381 atcactggtg ggactggccg gctctgctct tccttagcgg cgcctcttgg atttatcgaa
     1441 aggccgtcat catgaagtac cgctcctttg ccgaccacga ggagcggcag gaacggctcc
     1501 cggctgtcgt catcagcttg ggtaatatta ccgtcggcgg gacggggaag acgcctatgg
     1561 cctgtatgct ggctaaagaa ctttctgccc ggggctggcg cgtagcactc ctcaaccgag
     1621 gctatcactc gaaacgggag aagagcggcg cttcggtcat gtccgatggc cgcatggtac
     1681 tccttacggc tgaagagggg ggcgatgaag cgagtctcat ggccagaagc ctcatgggca
     1741 ttcccgtcct cgtcggtcgg gaccgggctg taagcggccg catggccgtt gaaaaattcg
     1801 atgctcagat actcatcctc gacgacggct tccagcactg gcagctttat cgcgacgtcg
     1861 acatcgtcct catcgatgga accaatccct ttggaaatgg ccatgtcctg ccgcgaggca
     1921 tcctgcgcga accgctggcg cat
//
LOCUS       DAWDXZ010000359         1936 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10515_length_1936_cov_5.405104, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000359 DAWDXZ010000000
VERSION     DAWDXZ010000359.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10515_length_1936_cov_5.405104"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    625..654
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          608 a          421 c          398 g          479 t
ORIGIN      
        1 tggcccgacc atgaacggta aatacggagt catcatcgga ttgatcgtcg ttttaatcat
       61 tgccatcgta aacacatatt cgcaaaagaa agctatgaat tgaggtgaga cacatgatga
      121 tagatgccag aggttattcc tgcccggaac cggttattat ggtaaaaaaa gctttagaac
      181 aacaaaggga cgacaaaacc tgtgaagtac tggtagacaa ccacgctgcc cttgaaaaca
      241 tcactcgttt cgtcacccac caaggatacg ccgtagaatc ggccgaacag ggagacgatt
      301 ttctcctgaa aatcacacgg aagtagtgaa agccatgtac tacgtcgcat tattttattc
      361 ccatttcggt gccattcgat tcatcaattc ccttcccgaa agcattgtcg agaagcggat
      421 gatgcctgtc ccccgctccc taagcgcatc ttgcgggacc tgcgtccgtt ttaaaggtcc
      481 cgatgactac acctttaccg tcgacgaaag tcatgaggtc gaacaaatcg taaaagaaac
      541 gaaggacggt tatgtcatcg tctttaaatc agaatagagc acaaacgaca aaagtaccgt
      601 tgctgtatac catgcagcaa cggtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnattaca
      661 atagggcaac agtgctttaa gctgggcaat gcttatgaac actcactgaa ttatctgtca
      721 ataattcgcc cctatatgca agaaattgta aatcgctgtg atgaaatctg tcagctaggc
      781 atattggatg gccccgatgt attatacata gaaaaaaaag agcccgacca agccatccga
      841 attacatcgt atgtaggaaa agccttggcc gcttctgcga cggccttagg gaaagtactg
      901 ttatccgggt tgagtgatga aatgattcgt gaattatata cccatggatt aacacgctat
      961 acagaaaaaa caaccgtcga tatagagacc ctattagagc aagtcaaaat cgttaaagca
     1021 aaaggatatg ctcatgaaat ggcagcgccg gagaatttta tgcatttacc tacgatgaaa
     1081 tcaaagcaat gataacagat gccgtaaagt atatcaacaa aagaggcttt gttttagccg
     1141 gcacaggtcg tatggcaaaa tctgaaacta tcagtctttc caacgccgtc attgacgccg
     1201 gtgctgacgc ggtcatcatt gtcggtccat actattgcgg atgtacacag gaaaatgtat
     1261 tccagtatta tgatgatgta atgggacaaa ttcatggtaa cattttcctt tataattttg
     1321 ccgatcgcac aggatatgat gtttcggaaa aaacgatact gcgactcatc aaaaaacact
     1381 cgaatttcat tggcgtaaaa gatacgcatc cgatgttacg ccatacacaa cgatacattc
     1441 aggaaataaa gagccagtat ccgaatttca tggtatttac gggatatgat aataactgta
     1501 tcccctctgt cctttccggc ggcaacggct gtatcggtgc tttatccaat gtctatccgg
     1561 aaatgtgcca tcaggcagct gaagcccttc gtaatgaaga tttatccgca ctccgctcca
     1621 gccaacgtca aattgataaa cgcatgaaat tctatgaagt atatccgacc ttcaacccgg
     1681 taatgaaatg ggctttaaaa gaattaggcc agcctatgca ggaaaattgc aaggcgccgc
     1741 tcgtcccatt aactgctgaa gaaaaacaaa aactaagttc tgccgccgat gagctttggc
     1801 gcaacaaata aaaataagct gccgttttga atcgaccctt taaagttgga ccaaaatcca
     1861 actttaaggg gtcatatcat ttgaactgct acccgtcaag tagacactcg aaaaaacata
     1921 aatagatttt gtttct
//
LOCUS       DAWDXZ010000360         1936 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10514_length_1936_cov_8.854333, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000360 DAWDXZ010000000
VERSION     DAWDXZ010000360.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10514_length_1936_cov_8.854333"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          468 a          545 c          532 g          391 t
ORIGIN      
        1 ggtcgaagtc tggacgcagg aagatctgca gctcatggga gcggcgaatg tccggtcggc
       61 cctgcaactg gccaataacg tcaacctggc ccgggcgggc atgacgggga acgccgtcat
      121 gattcgcggc atgagcacca accactcgct gatcctcgtc gacggcaagc gctatgccgg
      181 tgaagatacg gatgtcacga ccaacgtcta tgccctggaa cgcctcgatt tgagcgatat
      241 cgagcgcatt gaaatcgtcc gcggcccgtc gagctccctc tacggctccg atgccatggg
      301 cggcgtcatc aatatcatca ccaaggtccc cgataaggcc ggcggcactg taggcgtcgt
      361 caccggtacg gaaaatacgg cgtcatacgt caatatcgac tttggcagac accatcgctg
      421 gtcgacgtcc ttcgacgccc gctgggaccg gctgcggccc tttaaccgct acgtctacag
      481 cagcaccgtc tccggccgcg gcacgcggac cgtgaccgaa ggctatgacc gggccatgta
      541 cggcatgcgc cgcatgtacc atctggccgg cgtctatgac tttgaaaaca gcaacgacaa
      601 caagctgcgc gtcgacctgg agtatcataa tgatgacctg cggtccgatt ttgctccggc
      661 ctggtatgcc atggggccca tgaagcttcc gatgtcgctg gataagcggg aattttacca
      721 tgaacaggag cgggcagcca gcgtcgaata caccgggaag accggccgca atgactacat
      781 gttccgcacg tattacaatc agttgaagaa aaattctgtc atctacaatg accgccctct
      841 ctttccgggc atgatggaac ggatcttagg cggcatgtac cccagagccg atatggaccg
      901 ggctgattac agcacttggg tgaccgaagc ccgggatacg atgtatatcg gcgaccatca
      961 taacctgacc tttggcggcg aatatcggaa actgaaatac gaagggaccc gcctctccgg
     1021 ctcgtcggtc ggtcttgata aggatgtggc cgggcacgaa gtcaattcct atgccggcta
     1081 tgtagaagac ttgtggcagg tcaacgatcg cctgctgctc atcccgtccc tgcgctttga
     1141 gcacaacagc cagttcggca gtcagacgac gcctaaaatc ggcctgacgt ataacatcga
     1201 tcagaactgg cgctttaaat cgaattacgg ccagggctat aaggccccgt ccatcagtga
     1261 actctatatg gaaatgcacc gggccatggg agcgacgacg gtcaatatct acggcaatcc
     1321 cgatttggaa ccggaaaaat cgacgagctt cgacttcggc ttagaatacg ataaaggccg
     1381 caatttcggg aaattgacct atttccataa taaggtgacc aacttaatcg aaacagctcc
     1441 catcggctat atgacctatc agtacatcaa tatcgccaag gccgatatca acggcgtcga
     1501 agccgaagtg gggcgccgcc tcaatgaccg ctggaccgtg aagatgaccc ataactacct
     1561 cgacgcgacc aacggtacga cgggcgaacg cctcaattat cgggccagac atacgacgac
     1621 gctgcaactc ttatacgacg atcataaaga aaagggcttc agtgccgtcc tctgggatca
     1681 gttcagcaac gactaccgct ttgacggaca ggattatacc tataatacgc tgaacttctc
     1741 cttccgcaag aaactgagcg gcggctttgc cgtctacggc ggcgtcgatg atatttttga
     1801 caagaaagtc gacgacatct atgtcgacgg ccggatgtgg cggatcggcg ctgaatggaa
     1861 gtggtaaggg ggcgtcgtga tgaaatcctt gttacgaaaa tcattgacct tgtgggcttg
     1921 ggccctcgtc ctggcc
//
LOCUS       DAWDXZ010000361         1933 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10527_length_1933_cov_9.727370, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000361 DAWDXZ010000000
VERSION     DAWDXZ010000361.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10527_length_1933_cov_9.727370"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          433 a          511 c          526 g          463 t
ORIGIN      
        1 atacccttta tggaagaaga cgatgccccg ataaagatag gacgtcgggt cgtccctgtg
       61 gaggatgact ttttcaaagc ccgtcagtaa atcatcgtcg ctcccgtccg gcgtctgggc
      121 ttcggtccac atccagagga tttcctgacc gtagtgcttg ccggggacgg cccagcgacc
      181 attgaggccg gtccaattcg tcacctttcc gtagaggtcg ggatgatttt gaacgaggag
      241 ttcataacgg ggatctacga tagccgtttt cggccgttcc gggctggcat aagccaaaag
      301 atgctggata tgggcgcgga cgccgagctg cggcgtggca aaactggctc cgggggcccc
      361 gtttcccgtt gcaccgaggc cgcagaaatt attctgctca ggcgacacgt caccgccata
      421 ggcaaagtat cccgtctcct tcaaggcctg acagagggcg acgtcgggac ggatgccttc
      481 gcggccggct tcgtcgtaat aagcttgtac gacgtcttct aagggagcgt ttaatttagg
      541 attggcattg cgctgacgga tgaagcggac catctgcgcc tgagatactg tcgccggccc
      601 catgatcgta tcggagcctt ccggcacgtc gaggaagggc cgatcataag gcggcagcat
      661 ctgccagtga gccttctcga cagcctgttt gtcaacacct attttagctt gaaccggaag
      721 agacacgaaa aagggcaggc ccaacagcac tgcccccata aggcctgcca aaaggcctct
      781 tttcagctta gaattcacga tagatgccct tcttccgctt agcttccgta gatttagaaa
      841 tatacgtccc cagaactaag ggatagccct tgcgctcgat gatccgggcc atgtaattcc
      901 ggaagggaca gggcggccgg tggtacgagt cggatacgac gcacgaggcg aaatggatgg
      961 tgacattttc tttatcatcg ccgtagcgct tcatcttccg ggccaagttt tcgagcttgg
     1021 ctgaaatgcc ggcaccgcag cagccgccgc aggtcatcat gagaaaccgc gtatcggcgg
     1081 gataatcagc catggtgtcc acgcggttgg cgaaggtatt catacaggca tagccgctgc
     1141 agcgcttggt gacgatatcg cattggatga cgacgactaa ttgggacata aggttccctc
     1201 cttttagtta tatacgtagg tattcaagaa atcctgagtc caagccggat cgatgcgggc
     1261 cagcaccggt tcttctttcg gcagattttc cgataaagac tgttcaaact ggttgtacaa
     1321 ggggctgagg ccgtccttaa aatcaatata ggcgccctgt tccgaggcct tgtcgaagaa
     1381 taccaggcga agggagccgt catcctggct gacaaagtgg cagctcgtaa ccttaaagtc
     1441 cttatcctgt tccttaaatt gagccagggc tgcggctttg gtcaattcca tcaagcggtc
     1501 atcgaggccg gcttcaaaga ttgttatttt ttccagcaaa tcttctttcg tccggaccag
     1561 gcggaaaata tactgattgg cactcatgcg ttcaaagacc tggtgattgt ccgccatatc
     1621 acggtcagcc cgctgttcgt cattctcagt cggcccgtaa aacaccatga agcgacgcgc
     1681 tgcgtcgtga tataagaaac cgtagtcgat attcaccgtc gtgccgcagt gcgggcagtg
     1741 atagtgaaag gcgtctaagg acgcgatgct gtccttcatt tccgggtttt cggcgatgtc
     1801 gacactatcc caaacggtaa aattcccggc cttttgacag gcaggacacg tgatttcttc
     1861 ttggtgctgc atactcatga tgaatacctc tcttctctgc caccgtagat ggcgtttttt
     1921 ttattatacc aca
//
LOCUS       DAWDXZ010000362         1932 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10531_length_1932_cov_11.212573, whole genome shotgun
            sequence.
ACCESSION   DAWDXZ010000362 DAWDXZ010000000
VERSION     DAWDXZ010000362.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10531_length_1932_cov_11.212573"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          501 a          544 c          543 g          344 t
ORIGIN      
        1 acagatcgcc aaaaaaagag gcgacgggct gaagaaggcc tcggcccagg ctgccgtctt
       61 ggcatcggaa cttatcgacg ggaagccgtg cccggtctgc ggggcgacgg atcatcctac
      121 gcctgccatt ccgcccgagg atttaccgac gcgggacgac gtcgaacggc ggcaaaaagc
      181 agccgaaacg agcgaacagg agcggcaaaa gctggaagtt gataccaagg ctgccgaaac
      241 ggcctataaa gccttagaag gtcaatacga tcatctccgt cagcagtatc cgcaagacgt
      301 ctccttgaaa gaactggaag ctgaattgaa agcggcgcaa aagacacagg cagaccttga
      361 aaaggccctg gcccaagggg gaaatcttcg caaggccgtt tccgatggcg agacgaaact
      421 cagccaatgg gaagcggccg aagaagcggc aagaaaggcc ttagaagagg ccaaaacgag
      481 agccgtcaag gccggcgaga cccaggccca tttggaagcg gaactgcccg aggcctatcg
      541 caatcccaag gaactcagcc gccgtctggc cgagttgacg cagcacctta aagactatga
      601 agaagctctt cagaaaagcc gggatcatct ggtaagctgt gaacgtcagg gagctcagcg
      661 ccaggaagaa gccagaagcc tcacccagca ggtcctgacc ctgcgcagtc gattcaaaca
      721 ggactataac gacctcggcc aacgggtact ggcagcgggc tttgcctccg ttcgtgcctg
      781 ccgggacatg cagccgtccg taaaggatat tcccgtcctg gaagccgaag tccgtcaata
      841 tgagaccaag ctgcagcaag tacgaggcca gctcgaacag gaaaaatcag ccatcgacgg
      901 taagccacgt cccgatgtcc ctgctttggg gaaaaacttg gctgaagctg aaaagacgag
      961 ccgcgatctt gccgaagaaa ggggacgtct gtcggcgacc ttctcccaat ggcagcagga
     1021 aaagaagaag ctactggccc tcggtcagga cgaagcggat ctcgtcgaag cctataagac
     1081 cgtcggtgcc gtctacgacc tcattgctgg caaacagacg ggcatcaact tcgagcgcta
     1141 cgtcttaggg gccctcctcg acgacgtctt ggccgccgct aatgaacggc tccaggccat
     1201 gagccgtcag cgctataccc tgcagcgttc ccaaagttgg gatgataaaa gggttaaaca
     1261 gatcggcctc gacatcgaag tctacgacaa ctatacgggc tatgcccggc cggccaacac
     1321 cctgtccggc ggcgagacct tcttgtcgtc cctgtccttg gctcttgggc tggccgacgt
     1381 cgtccaggct tattccggcg gcatccacct cgatgccatc ttcatcgacg aaggcttcgg
     1441 caccctcgac agtgagactt tggactatgc cttaaagacc ctggcgtcgc ttaagagcgg
     1501 cggccgcctg gtcggcatca tttcccacgt gccggaatta aaagagcgca tcgacgcccg
     1561 cctggccatt cataaaaccg atcgaggcag tacgtcgtcc tttgaatttt cctaagctcg
     1621 tctctatatt gttgaattga cggaaaaagt atataataag gcataatcga gagaggaggg
     1681 acggaatgtc cattcaaaaa gtacgcgact atttttctca gtggggaatg gcagacaaag
     1741 tcatggaact gaccgagtcc agtgccaccg ttgaagaagc ggcagcagcc cttcatacgg
     1801 aaggtcggcg cattgccaag accatgtcct ttctggtcgg tgaccagccg atcctcatcg
     1861 tctttgccgg cgatgcccgc gtcgataacc ataagtacaa agagaggttc cataaaaagg
     1921 cgtccatgat ca
//
LOCUS       DAWDXZ010000363         1931 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10538_length_1931_cov_9.754264, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000363 DAWDXZ010000000
VERSION     DAWDXZ010000363.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10538_length_1931_cov_9.754264"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          453 a          523 c          479 g          476 t
ORIGIN      
        1 tctgtctcat gctgcagttt ttggctgaca aaggcggcca tgcagccacc ggcactgtca
       61 ccgcacaagg ttacatccct ttcgtaggca atgttcttgg cctccaaaaa ggaaagagac
      121 tgccgggcca cggctaaggc atcgtcgtag gcggcaggat agggattttc cggagccagg
      181 cgatattccg gagagatgac gatatggtcc gtcgccttgg caatccggcg gcagacggga
      241 tcatagaccg agaccgtatc gatgacgaag ccgccgccgt ggtaatagac gaggaccggc
      301 aggggccgac tcgtatgggg aatgtaaacg cgaaccggca ccggataggc cgggcacccg
      361 tcaaggacgc cgtcgacgac ggccgccatc tcaactttgg gcagcatctg ttcctgcgcc
      421 gtccggagcg atacttcccg catcgtcacg ggcgtcatta taaaatcctc cacctgatgg
      481 gcagcgataa aagctgccac ttcccttttg atagcactca tcataagcct tctttccctc
      541 tgttatggtt tcttatttta tcataaaaga atcggcctgt gaaccgcatt ttccggcaac
      601 ctttcttttt taggcaacaa aaaaagactg tccatgaaaa aagttcatgt gacagtctct
      661 tatggcgtcg tatggcggag agcgtgggat ttgaacccac gatagagcgt tcgccctata
      721 catgatttcc aatcatgctc cttcagccgc tcggacagct ctccgcgtct acataccctt
      781 tgttgtatct tcgggataaa tacggatatg taaaatatgg tcggggcgac aggatttgaa
      841 cctgcgacct cttagtcccg aaccaagcgc gctaccaaac tgcgctacgc cccgttgacc
      901 cgacttgttt attgtaccat atcccacaaa aaagtcaaga aaatcggggc attcatccga
      961 ctcggccgca ccggtaacat gaatcaaagg ccctaggcca atttcttctc gcgaatgaaa
     1021 tcaacgacca tgactgccgt atcggccatc aaggtttcgc aggacacggg accgccattg
     1081 gccgggctct ttaacacacg acagcacgtc gctttgaagc ggtcgctcca aattttctga
     1141 aattcccgaa cgggctgatt gatttcagcc tttgtctttt cctcgggctg cgtccggccg
     1201 acgagactgc ctaagacgag acaggctccg ttcaaggccc cgcagagaca gccggagccg
     1261 gcaacaccgc cgccgaggat ggataccatg cgcagcgttt catcgggcag atgcaaagcc
     1321 aggctgtcat tacagacctt gaccaccgtt tctgcacagt tattgccggc cttatgatag
     1381 gccacggcca gtgttcttat atcttccatc gtatgtcctc cttgctcttt cttatagatc
     1441 tatctatatt gtacctgttt ccctgcaaga tggccagact ttgtcgcagg aacgtcctcg
     1501 tccccctggc cgcctataaa agggcataaa aagaggatga cgacaaaatg atttccactt
     1561 tgtcgtcatc ctctttatgt taagattact gagctttggc aacttcgacg agtttcgtga
     1621 aggcagcagc atcattgaca gcaagatctg ccaacatttt acggtttaca atgacaccgg
     1681 ctttggtcag accatagatg aaacggctgt agctgaggcc gtttgcacgg gtagctgcgt
     1741 tgatacgagc aatccacaat ttacggaatt cgcgtttctt ggcacgacgg tcacgacgag
     1801 cgtagtagag cgctttcatg acgctttcgt tagctttttt gaagagacgg ctgcgggtac
     1861 cgcgatagcc cttagccatt tttaagattt ttttatgacg agcgtgtgct gtaacgccaa
     1921 ctttgattct t
//
LOCUS       DAWDXZ010000364         1926 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10556_length_1926_cov_9.505077, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000364 DAWDXZ010000000
VERSION     DAWDXZ010000364.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10556_length_1926_cov_9.505077"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1597..1681
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          406 a          487 c          417 g          531 t
ORIGIN      
        1 tccgtcactt cattggtgag gctggtaatc ttgttggtat tggtcgtgat ctgcgtcgta
       61 ttcgaagcta cctggctgtc taaagccgtt aagttatcgc ccgccgactt cgctttctta
      121 acataggttc cgtcttgagc gacccgtgtt tcattgtata ccgtaccgcc ggtgacgatg
      181 cccttattgc ccgaagctac cttgccgtcg gtattgacct tgatcttgta ttccgttccg
      241 ccgatattat tcttttctgt gctgtcgata gaaatcgtgc tgtcgccgtc agcattggca
      301 atggtatttc tcgtgtcgat tgccgaggca atggtcgata aattaacgct gcccgaaatg
      361 atttcggtcc cttccgagtt cttaatggac aggcctaatt tattgtcttt gaatgttaag
      421 gcttcgttac tttcctgtaa gcccgtgccg gctgcatcta cggccgcttt gagctgagcc
      481 acgttgacgg catcggtatc gttggtgccg gcagcgacgt tggtgatttg acgggtgata
      541 cctaattcac tgtcaccgac ggatacagcg gcagcattgg actgccaagc cgatgtaatc
      601 tgattctttt ccgatacagc tgccgtgtag gccttattgg catcgttcat gctggattcg
      661 gccacatcca tagcctctct ggcagcatca ttggcagcct tggcagcatc catctgctct
      721 ttaatttttc gaccttggtc tgaatcatgg tcaatgttgt tataagcatg tacggcctgc
      781 acatagtcag cgttcttggc caaataatcg gataccttgg tatcataatc agctttggct
      841 gtcgaaagag ccgattctaa atcagggata ctgttattta attcggcaaa gcgagcttct
      901 ttgccggcaa aggctacgat agccgcttca ttttcaaagg ctttgccggt aatcgggtta
      961 tacccaaaag cccctttgtc aacagaagat acagcgttgc tgccaatcgc cacgccgtca
     1021 tcatgctcta cgtccgtatt ataaccgacc atgacggcgt tgctgatgtt ttccgcatgt
     1081 tcgcgtaacg gtttttgcac ctttaccaca tagtgcagcg tatctccagt ccactgcagc
     1141 tctcctgtcg tatgagcgta actctgggga gtataggtct gttcttcata gccatctgtt
     1201 gtccccatcg ttcccaaaat gacgttattt ttgccgcccg ataaggtatg gtaatcaccg
     1261 atgacgacgt ccgtcgccgc atcgctaagc ttattacccg tccccatgac ggatacgtta
     1321 ttcacattgg ttgccttatt ctgatagccg gtaaccagat tataatcact ggccgcagct
     1381 tcgctgccgt ttaaagtgtt gcccgtccca aagatagagg aacgtcttgc ataatccgat
     1441 acgttgccgt tccctagtgc aaagacagaa ccgccgttaa gcgtagcata atcacccatg
     1501 atttcagaga aatgatcctt atacccgata tcatcaccgc catgatagag cagctcgccc
     1561 atacggccgc tcataaatcc gccttcaata ccttcgnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 ntggtcaccc ggttgccggt accaaagacc atggagttaa aggatgagtc aaccttattc
     1741 aacatgccga aagcaccggg agctatcgat gcaccaaatc cagtgtcact ttcagtactg
     1801 attttgttat cgataccata gagagtcgca taataagtag ttccctcgac ggtcgaacga
     1861 attcccacag ctgcacttgc aggccctaag gtcttaactc tactcccgat tgccatactg
     1921 ccatcg
//
LOCUS       DAWDXZ010000365         1921 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10581_length_1921_cov_8.075563, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000365 DAWDXZ010000000
VERSION     DAWDXZ010000365.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10581_length_1921_cov_8.075563"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          387 a          539 c          488 g          507 t
ORIGIN      
        1 gcgccactgc tccggtggtg tggtgtagcc ggcatgattc aagcaagtaa tctcaaacgc
       61 cgcgttataa gcgtgcttaa cgacgtctgc ggattgcagg tccgctaata cctctgtcgg
      121 gatagactgt cctgcggtga ggtccacgac tgcgacatcg ccgaagtcat aggcgtaggc
      181 aaaaagcaga atttcaaaat tatcagaatc gcagtattta aataatccac acttaccgat
      241 gtctatatcg gagtatgtct cgatatcgac gcttaagtgt ttcatgctta tcatcctttc
      301 agttcatccc acgcgtacgg gccctgctgc gggccgtgag caaatacgca gccggtgtct
      361 tgcgaataga acttgcagca gatgcattct gctgcgctgt gcgcccggca gtaccgccga
      421 atcgtctgcg cgtagccaaa cagccctagt tcgatgaagc taaacttagc ctcaagattc
      481 cagtgtgtcg gggccccggt cagtcggcat aatctgtaat cgttatcccg ccaagcgaac
      541 atacactcgt cgcaattcat ttcgtcgcag gccgctttca gcttgcgggc ctggatgata
      601 agtttccttc taggcatttc cagccctcct ttaggtatta aaaatggacg cgcggattcc
      661 cacgcgccca taacagtatt acagcacctc gccggtaagc gggttgatat tgcctacagc
      721 cggagcaggc tgaatgggct ggaagacgga tttagccgac ggcgccgatc cgcccagcgg
      781 ttcgccgtcg cgaactttct ggaccgggcc aaggccgcag cctacgcctt tcttgccttg
      841 gaaattataa ggaaagaaat ttacggagat atttgcgtat accccgctat agatttgtgt
      901 tgcgtccatg atgcggttga gctgcgcatc gacgacgtcg acaggcttat cagcggcact
      961 ggacgccgta aacacccagt gtcctttgca ttccggtccg aattcactgc cattctgagt
     1021 caatccatca ccgtcccaga cgggagtcgg gactctagcc ggtgcagttc cgttccattt
     1081 cccgttaatc ccgttgcgcg tggccgcggc aatagccgca tcaattttct gtttcccgac
     1141 ggtatccgtc ttcgggagca ggaccgttac ctgatacttc tgcggtgctt gcgggtcgcg
     1201 ggcataaggt ttcaagagat gcacataact caatcttacg ttttctacta ctacattagt
     1261 gatttccata attaatctcc tccattaatt aattttcaat tttcttaaaa acatcagcag
     1321 cgttttgtgt taatttcatc ggcggccgtt tgtcggattc cggggccaat gtaggcttgc
     1381 cggggcgctt ctctacgtag tcgcctacga gttccgtaaa tagtgccttg ccgatagcct
     1441 tttccgtttt ggccagggta agcggtacgc gctcatacag gaccgattct tcaatgccgt
     1501 tctgcatgag tttttcaaac gccgcatcgg cgtcggtaaa agcccgactt ccgcggccct
     1561 cgacgacttt ccagcctggg acgtggatgc cttgcagtcc acatgacagc ccgtactcct
     1621 gtaagtcatc tgcccactct ttgagcagct tagccgcttc caggtacttc ccgagttccg
     1681 gcatcgtgat catggtcatg tcgcgctttt cgtgcgcaac ctctaccata gcggcgtatt
     1741 tttctgcgcg ggccttgcat tgtgccttgg cccggcagaa cttgcaccat tcgccggggt
     1801 tgaatttccc gttgtcgctg gccgcttcct gggctttggg aattacgacg tcttcggccc
     1861 agcgttgcag tgccttgacc tctatcgttt cctgcccgta ggcgttaagt cgaggttgca
     1921 c
//
LOCUS       DAWDXZ010000366         1918 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10596_length_1918_cov_7.997316, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000366 DAWDXZ010000000
VERSION     DAWDXZ010000366.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10596_length_1918_cov_7.997316"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1663..1672
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          487 a          494 c          433 g          494 t
ORIGIN      
        1 caagaggccg cctgcaaaaa agtcttggaa cggataggac aataagagaa cgattgcggc
       61 tataatcagg atataattgg caatctggct ccgtcttccg ccatttgtgt tactcgccat
      121 aatacctcct atttatcgat gatgaggtca tccccatcgt gcttgggaat ccataagggt
      181 acgtcgtaag cctttttaaa ttcatcagac ggcgtccacg gcgttccaaa ataatgcatg
      241 ggaatgagac acttggttac ggaaacatcc tttaaaaatt ctttgacgcc ccattcacga
      301 gccccttcta agcgggcgtc aacagggaaa aaggtgatgt cgaaggtccg ccccactaaa
      361 tgcttcattt ctcgttcgaa attggccttg gcttcagcat tgttttcatc ggtgtcgcca
      421 agccagtgcc accaattcaa gtccccggca tggaaaatct tcaatccgtc tgtttcgacg
      481 aggaacgatc ctccttcgtc ggtcgatccg aattgggtga tcttcgtatc gccaagggtc
      541 agcgtatcat agagcttcat ggcatgaacc gccttgaggc cgtctcgttt caaaggcacg
      601 tcttcattga taatgtactg gtcgccgtcg aagtcggcaa tgcggccgtt aaagtggtcg
      661 tcatgccagt gcgtaacgaa aaaccagacc ggtttccgac ggatttggct gtaagccttg
      721 accgttcctg acggatcttt gtaataatca aagaccagaa ccttggaatc agtctcaaca
      781 gaaaagccac tatgatttaa ataggtaatc tgcgtgctca tataatataa cctcctttat
      841 cggttgataa atcattatgc atagtcttta ttataccata tatgcgctct cgatgtacat
      901 cgtgccagcc gtccttcccc gcagcgaggc catttttctc ctccctaaac ggagaaaaaa
      961 acaggttcat catcgtcatt tttaataaat caccaaattt gtcatctttt tacaaaaatt
     1021 ctattgacaa acggaaagcg aaggcgtatt atgttctcaa accaaagaca taaatgcaat
     1081 gattgaaaca agtacacaca ggaaacggta cagagagtag gggtcattgg ctgaaagccc
     1141 ttatcaaagt caactgtgat ggaatgcctt caagagcagg atgattgaac ttaacagtag
     1201 gtcatctcgg ttgccgccgt tatgggtatt gagtggcagt cttcgtactg caatcagagt
     1261 ggaaccacgg accatcgtct ctgtatgacg aggtcttttt tttattcatt ttttcagact
     1321 acaggagggc gcccagccat gttcaaatca cttcgcaacg acattcgcgt cattaaggac
     1381 cgcgacccgg cggccaaaaa tacgcttgaa gtcttgctct gttattccgg ccttcatgcc
     1441 atttgggctt accgcctggc tcacttcttt taccgccgcc aatggttcgt cacggcccga
     1501 attatttcca ccatctcccg tttctttacg ggaatcgaaa ttcatcccgg tgctcaaatc
     1561 ggtgaaggac tttttatcga ccatggcatg ggaattgtca tcggcgagac gacgatcatc
     1621 ggcaacaacg tatccctcta tcagggcgta accctcggcg gtnnnnnnnn nngaccatcg
     1681 gcgactacgt cgtcgtcgcc tgcggggcca aagtcctcgg ttcttttacc gtcggtgaag
     1741 gcgcaaaaat cggggccggc tccgtcgtcc tcaaagccgt tccaccgcac tcgaccgtcg
     1801 ttggtattcc cggacgggtc gtcgttcaaa agggcaagca cctcaaagcc agccacagcg
     1861 aacgcgacgt cgacctcaat cacaatcgcc tgcctgaccc catcgaagat cagattgt
//
LOCUS       DAWDXZ010000367         1918 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10593_length_1918_cov_10.362319, whole genome shotgun
            sequence.
ACCESSION   DAWDXZ010000367 DAWDXZ010000000
VERSION     DAWDXZ010000367.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10593_length_1918_cov_10.362319"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          418 a          506 c          453 g          541 t
ORIGIN      
        1 tgttcgacat cacattcttc aggaatggtc ttgacatatt cttctatagc ccgtgctgcc
       61 tgtgtatggc ctgtaccgat cgaggccgac aggatcagga cttttttctt tgccatatag
      121 gatcccttct tttataaaca gccttcgttg caccttccac tatatagtac gataacttgt
      181 atgaaaagaa aagggccgtt tttcatgcgg cccttcggga tcgtacttat ttgttattaa
      241 ccaaatcttt aagctgtttg ccggctttga aagcgggagt cttggaagct tcgatctgga
      301 taggttcatt gttgcgaggg ttgcggcctt cgcgggcttt acgctggcgg acttcaaacg
      361 taccaaagcc gatgatctgg actttatcac cctgggtgag gctgtcgcta atggcttcga
      421 atacggcttt tacggccttt tcagcgtctt ttttcgtcaa atcggatttc tgtgctactg
      481 cagcgattaa ttctgttttg ttcatgttac taggtcctcc tttggatcgt cacatcttcc
      541 taacactaac agtctagagt ttttcctgcc gttctatccg cttggcttcc tgccagaatg
      601 agtccaactc atccagtgaa aatgcctccc atggccggcc cgaccggttg acccgatcct
      661 cgacatgagc aaaacggcga cggaatttac tgttagcccg atgtaacgca cattccggct
      721 ctatatgata gcgtcggcat aaatcagcaa atacgaacaa gacatcccca gcttcttctt
      781 ccgcatggac aggatcgcct tcatcaaggg cctgacggaa ttcagtccac tcttcttgga
      841 acttttccca gacaccttcg acatcgggcc agtcgaatca gactttggcc gctttctcct
      901 gaagcttgtc ggcagtcagg agtgacggca ggccgggaac gactccgtcc agcagatgag
      961 cgtgctgctg gcgttgttct ccttgcttta atctatccca gtttaacata ctcgtcccta
     1021 tattttcaag gcttttttgg ctaaaaacat agggatgacg ccgaatcatc ttatttgtga
     1081 tatcttttac gacatcctgg gccgagaaaa gccctttttc ttcggcaatt cgggcatgaa
     1141 tggccacctg atagaggata tcccccagtt cttcgcggat accgtctata tcgtcattgt
     1201 caatggcatc gagcatttca tagacttctt cgatgagata tcgccgcagt gtccgatggg
     1261 tctgggaccg gtcccaagga cagccgtctt cgcggcgaag tctggccgta acttccgtaa
     1321 ggggcatcaa atcaaaaggc agttgttcct tggccattcc ttcttcctgc cgcttctgat
     1381 cgatgaggcg gcgcacggct tcagccatat ccggtccgcc gggaacagtg tgaacgacgg
     1441 catgaaggcg gctctctttc aacaagaggt cgacgatggg tgcatacgtt tcagcaaagc
     1501 ttttcccgtc ctgcccgtag gtatcgaggg gacggtcatc tttccctaaa gcgatgattt
     1561 taatttccat gaaaggttcc ctcatttccg tcaaaaggcc ttacgcctgt cattatacct
     1621 cggcctgccc catttgtcgc gatgctgcaa agccggcttt accgcacgca ggctatgaaa
     1681 aaagctgaaa aggataccct ttcagctttc gaatgatgat atggggatca gccgctcaac
     1741 ttagtcagca aaaagataag ctgcgtctgt tcggcttcac tgaggcggct catgagcatg
     1801 gaaatgcgga gataatgatc tggcagcaca tcgtccataa actcccgtcc ttgggccgtc
     1861 aggcgaactt tcttgcccct tttatcggtc tgatcgatgg ccacttccgt cagtccgt
//
LOCUS       DAWDXZ010000368         1912 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10621_length_1912_cov_9.358104, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000368 DAWDXZ010000000
VERSION     DAWDXZ010000368.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10621_length_1912_cov_9.358104"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    971..980
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          438 a          481 c          524 g          459 t
ORIGIN      
        1 cgaagaaaaa taccattatg ctgaatcgac tcatgaagcg acggtcatta cgtcgtggaa
       61 ccgccaaaag tatgaagatc agtatgagta ccagtaccag cacgattcgt atgatgcggc
      121 cattgccaat gcctacgcct atcgccgtga ttttggcaag agcaaccttc gcaagtggag
      181 cagcaccgaa gggatggata cgccgtggga cagcaatcac acgacggata cggttcattt
      241 ccagagctcg accattgcct ttttggatcg gttgaacatg gaagcccgtc tggccggcat
      301 ttccttcatc atcaccggcg gcgctgaagg cgggtaccat gccagcggac tctattccca
      361 tgaaaatggc tataaagtcg atatctccga tgacggcatc taccagggca gtaaggctta
      421 tgaagtcctt cttgcggcct tagccccgta caagcatcaa attacacacg aatgggataa
      481 gaatcacttc gatatcacca tttatccgga aaactaccgc ggataagcgt ttcctttcag
      541 aggggaggga actcttgatg cggtcggagg gaagactgtc tgcaagggca gtcttttttt
      601 gatgataatc ctgcctttat aaggcttttt taccaggaga gagcatatga tgataccaga
      661 ttgggacctc tatttttcat ccttgattgg cgatgaattg cttcaagttc cgggactggc
      721 cctcgtcgcc tatcatgaag gccgcgaggt ataccgcttt gccggcggta tccgaagatt
      781 ccggccgaaa ccgttgccga tgacgatgga cagccgtttt cgcatagcct cggtatcgaa
      841 gatgttcact atttttaccc tcatgcagtt agtcgaagcg ggcgtattgt ctcttgatga
      901 cgacgtcagc gactatcttg gctttcgcct gcgccacccg gcctatgacg acatcccggt
      961 gaccgtacgc nnnnnnnnnn gcatacgtcg gggctgcgcg acggaaaggt ctacagcatc
     1021 ccgccattcg tttccgcaga ggcctttttt cagcctgaca gcccctattg ggaaaaggga
     1081 cagcactttg ctccaacggg agaaatgccg ggacagtatt ttacctactg caacttaaat
     1141 tacggcctct tgggaacggt catcgaagcc gtgaccggtc agcgctttga ccagtatcaa
     1201 aaggggcaca tcctaaagga tctcgattgc cgaggcgact atgtgccggg caacctgccg
     1261 caggctgcat ttgaaaaact gggtactatt tacagtaagg acgatgaagg cggcagctgg
     1321 caggggcgga tcgatgattt tgaaggccgg cagccggaaa aagagacagt tgcccttcaa
     1381 aatccttatg cagaaacggt ctgtcagacc tataagctgg acggatatcg acgggggacc
     1441 aatgccacct ttttttcgcc tcaaggcggc ctgcgcctgt ctctggcaga aatggggcag
     1501 actctcgaga tgatccttca tgacggggag tatcgagggc ggcgaatcct tagtccttct
     1561 tccttacggg agatcttccg gcctcagtgg cagtatgacg gccacaacgg cgatacctgt
     1621 ggtcagacga ttttttctta tggcttaggg gcctttttgg tggatggtac cggcccggct
     1681 cgtccctgtc gggagaaaga cttttctctt tggggacaca cggggcaggc ctttggcctc
     1741 ttgtcgggaa tctttgtcat accggggacg aagagcggtt ttgcctacgt catgaacggc
     1801 gagggagttt ctgaagaaga tccgcgaagc gccggcgttt tcagtaccaa ttatatctgg
     1861 gaagaacggg ttctcgacgg tctgtgccgt tttattgacg gcctctaggc cc
//
LOCUS       DAWDXZ010000369         1910 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10632_length_1910_cov_7.492722, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000369 DAWDXZ010000000
VERSION     DAWDXZ010000369.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10632_length_1910_cov_7.492722"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1402..1411
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          364 a          590 c          447 g          499 t
ORIGIN      
        1 ttttccaatt catcaaattc ggcattatgt tgctttataa cggataaaga tataggcaca
       61 caaaattcaa aggaggttct ttttgtatga acggtttata tctcgtcatt atttctgcct
      121 tagtgttcgt actggcttac cgtttttacg gcgcattcat tgccgccaag gtattgaccc
      181 tgaaccagta caaagttaca ccggccttcc gttttgaaga cggtcacgac tacgttccga
      241 cgaacaaata cgtcgtcttc ggccaccatt tcgcagctat cgccggtgcc ggcccgctgg
      301 tcggtccggt catcgcagcc cagttcggct acctgcccgg tgccttatgg attctcatcg
      361 gcggcgtcct cgccggtgcc gtccacgata tggtcatcct cttctgctcc atgcggtacg
      421 acggcaagtc cattgccgaa atcgccaaag cccagatcgg tgacaaaaca ggcttggcaa
      481 cgtcctttgc cgtcttgttc ctcaatatcc tctgcatggc cggcatggcc gtcgtcatcg
      541 ccaacgccct gcacgacagc ccttggggta cattcaccat cggcatgacc attcctatcg
      601 ccatcttcgt cggtatctac atgcagttcc tgcgtcccgg caaaattaaa gaaggcacca
      661 tcatcggcgt cgtcttgacc ttgctggccg tcgtcgccgg ccctgccgtc caggcttcgc
      721 cgaccttggc tcccctcttt accatcaatg ctacgggcat ctccatcgcc ctcatcatct
      781 acggcttcat cgcagctgcc cttccggtat ggctgctcct ggcaccgcgt gactacctct
      841 cgacgtacat gaaaatcggt accatcggcg ccttggccct gggcatcatc atcgtcgggc
      901 cgaacattca gatgccggct atgacccagt ttacggctgg cggcggcccg gtcatcaccg
      961 gtgctgtctt accgtatatc ttcgttacca tcgcctgcgg cgctatctcc ggtttccact
     1021 gcatgattgc aacgggcacg acgccgaaaa tgctgatgaa cgaacgctcc atcctgcccg
     1081 tcggctatgg cgctatggtt acggaatcgt tcgttgccat gatggcttta atcgcagcct
     1141 gctcgctggt accgaatgat tactttgcca tcaactcttc ggccgctcat ttccaggccc
     1201 tgggcattca agtcgtcgac ctgccgcacc tgagcaacat ggtcggtgaa gacgtcgccc
     1261 atcgtccggg cggtgccgtt tccctggctg tcggcatggc ttatatcttc tccaacctgc
     1321 ccggcctcga tcacctcatg aactactggt accacttctg catcatgttc gaagccttat
     1381 tcattatgac catcatcgat gnnnnnnnnn nactcgtcgg ccgcgtatgg cctaagttcg
     1441 gcgatccgaa ctggaaaccc ggtgccatca tcgcttctgc tttgatctgc ggggcttggg
     1501 gttatttagt cctcaacggc aacctgtcca ccatctggcc gatcttcggc gtatcgaacc
     1561 agctcctcgc tatcatcgcc ctttcgattt cgtcggtcgt cctctgctct atgggcaaag
     1621 ctcgttacct ttgggttacg gccattccct gggctttcct gatggtcatg atcttctggg
     1681 ctgactatct caacatcttc gaaatttacc ttccgaaggg tgaatggacg atgttcaccg
     1741 tttccatcat catggccctg ttggtcatca tcgttgccgt cggcgccgtc cgcaagtgca
     1801 tctacttagc aaagacggtc cctgccaact atgacacttc ggaaatcgtt gaaacggaag
     1861 aactgaaaca cctccaggaa ctggtaaaaa ctgataaagc agctcagcgt
//
LOCUS       DAWDXZ010000370         1898 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10688_length_1898_cov_6.473684, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000370 DAWDXZ010000000
VERSION     DAWDXZ010000370.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10688_length_1898_cov_6.473684"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          363 a          504 c          539 g          492 t
ORIGIN      
        1 ggtcccgcgc agggtaattt caccggtcat agccacgtca tggcggaccg ctttgccggt
       61 cagggccgat gtaatggccg tagccatggt aatcccggca gacggaccgt ctttcgggat
      121 ggccccttcg ggcaggtgaa tgtgaagatc ggtcttggta tagaaatctt cggcaaggcc
      181 caaagcttcg gcacggctgc ggatataggt aacgccggct tcggccgatt ctttcatgac
      241 actgccgagc tgtcccgtga gcatcaggcg cccccggccc ttgacggcta cggcttccgt
      301 ttccaggact tcgccgccga cctgggtcca agccatgccg gtgacgatgc cgacttcgtc
      361 gtctttatga accgtgtcgg gcaggaattt caccggccct aagagttttt ccaagttctt
      421 gaccgatacg ctgaggggca cttcatcgtt catgagcagg gattttccga tgcggcggca
      481 aacggtgccg atcatctttt ctaagcggcg gacaccggat tcgcgggtgt aaccggagat
      541 gatgcggcgc atgacggcat cggagaattt agcctggctg gccttaagtc ctttttcctt
      601 aatctgtttc ggtaccaggt attttttcgc aatctggact ttttcatctt ctgtatagct
      661 tgtgaagtcg atgatttcca tgcggtccat caaaggcctc ggaatattgc tgagaacgtt
      721 ggccgtcgtg atccagaata ccttggacaa gtcaaaagga agttccagga aatgatcgct
      781 gaaggtatgg ttctgttccg gatcgaggac ttcgaggagt gccgacgacg gatcgccgcg
      841 catatcggcg gtcatcttat cgatttcatc gaggaggaag accggattct tgacgccggc
      901 ctgtttcatg ccgctcagga tgcggcccgg caaagcaccg atataggtcc ggcgatggcc
      961 gcggatttcg gcttcatcgc ggacgccgcc taaggttttc cccgttcccg gagggcctac
     1021 gaagcaaaga atcggaccct tgaggctgtt ggtcagctgt tttaccgcca agtattcgat
     1081 gatgcggtct ttcactttat tcaggccgta atggtcttcg tcaaggacct tctgggcttc
     1141 attgaggtcc agtcggtctt ttgtttcttt cttccacgga agggccatgg ccaagtcgag
     1201 ataggtccgg ataatcgctg attcggccat catgggcggc atggaatcga ggcggtccgt
     1261 ttctttgagc agggcctttt cgatatcttc gtccagtttc atcttcttga tcttatcgcg
     1321 gagttcctgg acttccgtat ccttatcgac cttgtccccc agttcgtcgt ggatggcctt
     1381 tatcttttca cgcagatagt actctttctg ctgctggtcc atcttaccgc ggacttcgcg
     1441 gtttaacttc gattcgaggt tggcaatgtc gacttctgta tcgaggaggg atccgatgcg
     1501 gcgcagacgg gcttcgacgt cggtcatttc cagaatctgc tggcggatga ccaatttcaa
     1561 gggaagctgg gacgctgcat agtcggccag gacgcccggt tcttcgatgc tcttggcaat
     1621 ttcgctggct tcttcaccgt cgcgaagctt atcgagccaa tcgaggaggc gtttgaggat
     1681 gacccggcgc aaggcttctt cttccgtcgc attgcggctg acttcagcta ccttaatggc
     1741 agcgccttcg agatagcctg ctttctgaga aaagccctgc atgcggacgc gagaaatccc
     1801 gttgaccaag atgcggacca aaccgcccgg catgcgcagg aattgttcga ttttaacgac
     1861 gcagccaatg gtatagacgt catcaaagcc gggattgt
//
LOCUS       DAWDXZ010000371         1897 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10699_length_1897_cov_5.431596, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000371 DAWDXZ010000000
VERSION     DAWDXZ010000371.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10699_length_1897_cov_5.431596"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    913..922
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          473 a          379 c          546 g          489 t
ORIGIN      
        1 ataggcatcg gtggcggcaa tgacactgaa taaggtcgaa cgatccgtcg tgctgatttc
       61 ccgttccggt ttgatatcag gaatgatgac ttcttcggaa aactggtacg aatcttcttc
      121 ctgcgtatat tgaatataag gataaggccg gagctgatca atcgaaagcg acgggtattt
      181 tgccaaggga tggttggctt ggagaaagac ggacggaggg tacgcaaaga gctcggtgaa
      241 tttcagcctg tttttgcgca gcgtccgggt catgaatttt tgcgtcatct tggagatgaa
      301 gatgatgccg atttggctgc gctgggtgac gacgtcctga atgacctggg acgtacggcc
      361 ttcccggaag gtgatggcat actgcggctt tttggataac gtttgcaggt agtccgtaaa
      421 ggcatcgacg gcaaacatat agtgctggga cgagatggaa aggcgcagct tgttcttact
      481 tggctggtgg ggattgaaat gttcccgcag attggtcgtc tgctcgatga tgtgattggc
      541 gtaattaaga aattccaggc cttccggggt aaaggtgatg ccgtggcggt tccgttcgag
      601 gatggtgata tggaattctt tttcgagttc cttgatggtc gtactcagac tgggctgggc
      661 aataaaaaga gcttcagccg cagctgagat ggaattgtgt tttgataact caacgatgta
      721 ataaagctgt tgcagggtca tgaaaggcac ttccttcgta tagttgatgc agtatgatgc
      781 tctcggtgat gatggatagt cttattttaa cagagtcgac gatactttta aaggaacggc
      841 cttgtgtatg gaacgcggcg gcccaatgat gggtgcccga tggggtgaaa actgctgttc
      901 aggggaggca gcnnnnnnnn nngcccatca cggcgatgaa gaagcacaga gtgtggaagc
      961 cgtcttggtc gctgctgctg acgctgtatc ggccgctcgc ccgggcgccc gtcgggaaac
     1021 gttggaaaac tatctgaaac gcttgacgaa gctcgaagaa attgctgagt cctttgatgg
     1081 tgtcgaagac tgctttgcca tccaggccgg acgtgaaatc cgggtcatgg tcaaaccgga
     1141 taaattggat gaatccgatt gtgtcctctt gacccatgac atcattaaac gtattgaagc
     1201 tgaactggaa tatccgggac agattaaagt tgtcgtcatt cgcgaaacgc ggatggtcga
     1261 ttatgcaaaa taaccttata tgacttgcag ggccgccgta taggcggcct tttgcatgat
     1321 gcggcaaggc gtagaagcac ggatcgaaat gtcaggccga attgtctcga gtgtcctgat
     1381 tggtattgca attaatcctc tgaaaaaata ttattgaatt taaaaaataa tacttgccaa
     1441 agtcatcgtt ttgtagtaga atagcttttg ttcgaggcac taagaaatac cgagacagcc
     1501 ccgaagaatg aaagcaaatg gccccgtggt gtagcggttt aacatgtcgc cctgtcacgg
     1561 cgaagatcgt cagttcaaat ctgatcgggg tcgccatatt ctttttttaa ttttatatgc
     1621 ggttgtggcg gaatggcaga cgcgctactt tgagggggta gtgaacgaaa gttcatgggg
     1681 gttcaagtcc ccccaaccgc accacctgaa cggtcctacc gctatgcggc aggaccgttt
     1741 tttataggca tgaatccact ccgaacggag gaaatacgca tggataagga acacgttttt
     1801 tttgagaact atgctaaaca ctgggaccgt gaccggaaag agaatcggac cattttgtcg
     1861 gaatttttaa ggctgatggc gcttgaaaca ggggcgg
//
LOCUS       DAWDXZ010000372         1896 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10707_length_1896_cov_6.877784, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000372 DAWDXZ010000000
VERSION     DAWDXZ010000372.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10707_length_1896_cov_6.877784"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          410 a          460 c          493 g          533 t
ORIGIN      
        1 gatatggaca gcagttactg gtctgcccga tacgtaggcg ctaagcgcat catgtagtgt
       61 aagaaaaact cctacagaaa aatcagaatt tttctgctac cgttgttaga atttgtttat
      121 tataataaag ccgtaccata cggatttatc attattcgga ttcatgcaac ggaaggagtt
      181 ttttttatgc tcaatttaat ctggaacttt ttcagaacgg caccggacgc cgtaccaatg
      241 acggatggcg aggccatcaa gaaaaagttt aacgccatgc gttggcgcgt atttgcttcc
      301 atcacgatcg gatatgcttt ttattacatc atccgccaga gttattcggt tattaagaag
      361 ccgcttctgg catcggggat cgttacgccg acggaaatcg ggatcatcgg ctcggtattc
      421 ttcgtaacct acggtcttgg gaaatttacc aacagtttcc tggcagaccg catgaataac
      481 aagcgattct tttcctttgg cttgttcatt tcttcactca ctatggtcgg catggggctt
      541 gtcaattcct tcgttccctt agccgtcttg tggggtctta atggctggtt ccagtcttat
      601 ggtgccggtc cgtccattgt ctccctgaat cagtggttca gcaataaaca gcgcggaacc
      661 ttgtatggca tctggtttac gagtcacaac ctgggctcat ctttcgctta ctttgccatt
      721 gctgccatcg tttccatgta caactggtct atgggcttca ttgcggccgg catcatctcc
      781 cttattggga cggccttcat ttacttcggt atgagcgacc gtccggaaac gcagggactg
      841 ccgaacggcg ccgtctatta cggagaaaag acgcaggaac agatcgatgc ggaaaaagaa
      901 aaatcagtcg gcaccatgca gtggaatgcc gtcgtcaaga atcctgccgt ttggattctc
      961 ggtctttcct cactctgtgt ctatattgcc cgctatgcca ttgaaagctg gggcgtcgtt
     1021 tatctgacgt cgcagaaggg ctatgatatg atgggggctg ccggcgtcat ggcttatatg
     1081 caggtggccg gtatcttcgg ggcgctcttg tgcggtgtta tttcagacaa gttcttcaat
     1141 cacaagcgca atatgccggc cctgttatat gggatcttct attccctgtc cattgcagga
     1201 ttcctctggg cgccgcagtc ctttaccatg gatatgctgt gcatgacctt ctacggcttc
     1261 accatggggg ctttggtttg ctacatgggc ggtctcatgg ctgtcgatat cgtgcccaaa
     1321 cgtgttaccg gtgcggctat ggggatgatc ggtctcctca gctatgccgg cgcggccctg
     1381 caggaattca ttacggggaa actcatgacc attaccgtcg tcaatggcgt taaagtctat
     1441 gacttcggac tggctacgga attttgggtc ggtgcagccg tcgtctcgac gctgctggct
     1501 ctcctcgttt ggaatgcaaa accgaaagaa gactaaggat gaaggcgttg tccgaaggga
     1561 cagcgccttt ttttcgcata gccttgcggc agattgtaaa accctactca gccgggccga
     1621 ttcagggtat aataaatgat ataaattata tgcaggggag gcattatgaa acgggttatt
     1681 tctatgatgc tgctgacctt agcctgtctt ttcagtgcgg cctgcgccca tcctcagggc
     1741 tatatcgact ttacgcagcc tgagggcgct cgcccggctg tagagcagtc cggtgtaaaa
     1801 ccgcttcgca tcgcctttgc gtcggtcatg tcgccgaggg aaacgcggca ggtgtatcag
     1861 cagatcgtta cttatattgc tctaaagagg cagcgg
//
LOCUS       DAWDXZ010000373         1894 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10718_length_1894_cov_6.263187, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000373 DAWDXZ010000000
VERSION     DAWDXZ010000373.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10718_length_1894_cov_6.263187"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1392..1401
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          462 a          551 c          418 g          453 t
ORIGIN      
        1 aaattcgctg gaaaaccgtt tcactcctta tcacctgctt tgtttcaatt aagctgatgt
       61 atttatatat tctctgtcga cctgtccaat tccttctttt tgaaaggccc ttttcgcaga
      121 aatgccgaaa acggatacat agcctttcta tgttatacta tttcgacgca tagcgtcaag
      181 tttcctgttt atgaagatga aattttctcc attttctcaa aatcagcgta tttccttgct
      241 tgcgggctaa aatatagccc acctcttttc ctgaaatgtg tggatttatt tatttctatt
      301 gggtataata aggatagaca acgccaagat agaggaatat gccaatgatt cattttacag
      361 tcggtccgct ccaccggcct acgaccgtag ccggcgcgat gaaacacttc ggcgtctcat
      421 ccacactaag acgccgcatc aagcacagcg gcagctgtac catcaacggc cagcctgcct
      481 cgacgcgcga tttcgtccgg gaaggggatg tcgtgtccat cgtcattccc gtcaaaaacg
      541 aatttgcacc cgaggatatc cccatcggca tcgtctacga agacgactac ctcatggtca
      601 tcgataagcc ggcaggcatc ttaatgcatc cgacgtcggc cgttcgagac gggacgttgg
      661 ccaacgccgt ggcctatcac tacgccgcga cgggtcaaca ctgctcatat caccccatgc
      721 atcgcctcga taaaaatacg tcgggcctgt gcatggtcgc caaagaaccg cagattcagt
      781 acgccttcga caaaaaagat ctcagctata aacggctgta tctggccatt tgtcagggac
      841 gataccctgc gcgggtcaca actgtacacg cccccatcgg aagatgtccc gacagcatca
      901 ttacccgctg tgtcaggctc gacggcaagc cggctcattc cgatttcacc tgcctggcga
      961 ggtcctctga atacagcctg ctccaagtgg tgcttcatac cggacggact catcagattc
     1021 gggtccacag cagttacctc ggctatcccc tcttagggga cgacctctac ggcggttccc
     1081 gccgactcat gcagcgtcag gccctccacg cctactgcct gcagtttacc caccccatga
     1141 cacatcgctt cattaccgtt aattcccggc tgcctgaaga tatggacgcc ctcgtccgtc
     1201 aggccggctg gaattatata cattaacact attctatacg taacttgtag gaggaatgta
     1261 aacatgccat taattggaac tacagaaatg tttaaaaaag cctatgaagg ccactatgcc
     1321 atcggtgcat tcaacatcaa caacatggaa atcgtccagg ctgtcacaga agctgctgct
     1381 gaattgaaat cnnnnnnnnn nccccgatca tccttcaggc ttcggccggt gcccgcaaat
     1441 atgcgaaacc gtcgtacttg aaacatctcg tagccgcagc cctggaagat tacgatctcc
     1501 ccatcgccct tcacctcgac cacggtgctg actttgaaac ctgcaaagac tgcatcgaca
     1561 gcggattcac gtctgtcatg atcgacggct ccaaacattc tttcgaagac aatattgctt
     1621 tgacgaaaaa agtcgttgaa tatgctcatt ccaagggcgt cgtcgtcgaa ggcgaactcg
     1681 gtcagctggc cggcatcgaa gacgatgtca acgtctcggc tgaagaagct cactacactc
     1741 gtccggaaga agtcgaagaa ttcgtatccc gcacgggcgt cgattccttg gctatcgcta
     1801 tcggcaccag ccacggcgct ttcaaattca ctccggaaca gtgcacgcgc aacgccgacg
     1861 gcgttctcgt accgccgacg ctccgcttcg acat
//
LOCUS       DAWDXZ010000374         1888 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10744_length_1888_cov_6.803601, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000374 DAWDXZ010000000
VERSION     DAWDXZ010000374.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10744_length_1888_cov_6.803601"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          466 a          448 c          470 g          504 t
ORIGIN      
        1 taatattttt tcagatatat gcctaaacag cagtccggat actaaaattt tatctatggc
       61 attatgcata ttttcaatga aaaaccagtg gaatcgatat cgcccgtctg tccccggatt
      121 taccgaatta agaatgtgaa aatttcacat agataaacag atatcccctt agggaagaat
      181 ccttccctaa ggggatatct gtttcgggag ttattcaatc gtttcaataa gggtctcaaa
      241 ggaatctaat acttcctcga tggttttatc attgaggacc cggtaatcgg cggcattgcg
      301 atagaggcca atgcgttcgt cgtaaaggtc gtatactttc tgttttccgt cctttaagag
      361 gggccgcgtc gatacgtcga catcactgac gatgtcatca ggtgagcggt cgaggaagaa
      421 gacgattccc gtctgtttaa agatttcgat attttccgag cgcttgacga cgccgccgcc
      481 gcaggcgacg accgtccctt ctttagccgc gagcgtcttg ctggcccgta cttcggcatc
      541 gcggaagcaa tcttcactga cggcaaagag ttcgggaatc gtctttcctt cttgggctac
      601 gatgacgtca tcggcatcga aaaagtcccg tcccagacgc tgtgccaaag ctcgtccgaa
      661 agtcgtctta ccgcttccgg gcataccgat gatgatgatg ttctgtttat ttttcataat
      721 aatgttccat ctcctttgca atctgttcaa taacggtcga ggcaatttcc tgatccatcc
      781 aaatttcttc agagcctatg gcctgggcaa cgagcatgta catgccgttt aaggtcttgg
      841 caccatggcg ctttgcggcc tgcaaaatgc aggtatcttt cggattgtat acgatatcga
      901 cgacggccgg gaacctggcc acagccgctt ccgatatggg acaaccgtcg atattgggaa
      961 acatgccgac aggtgtgcag tttaccagga tatcgccgcg gcaagacgtt tcaaaagtat
     1021 gataatcgat agtttcgacg ccgatgcgtt tcgtaaaggc ttcaaagtcc gtctttgaat
     1081 gaggtctccg cgatacgacg acgatggatt ttgccccgta atcaaagagg tattggatga
     1141 tagcccgggc tccaccaccg gaaccgagga cgacgcactc cttatctttc acagagatgc
     1201 cggcatgggc caaagatctt ccgaaaccgc agtaatccgt attgtatccg aaggtaccgt
     1261 gatcggtaaa gtggatcgta ttgacggcgc cgatgatacg ggcttctcga gagatatccg
     1321 tcaaataggg cataacgtct aatttataag gaatggtaac gttggccccg gtatagcctt
     1381 catggcgaag gcgccgcagt tcattgggaa tgtcgttgcg gtccatttct aagacgccgt
     1441 agcgagattt atcctgaact tgtaagatgc gaaacagttt gtcgtggatt gccggagaag
     1501 ccgtatggcc cagtttagct ccgatgacgc ctaacttcat ctatatccct cctcggtcgg
     1561 atacgtagtt gcctagaatt ttaaagtagt tgcagtgttc tgccagatta tgcagtaccc
     1621 gggccgttgc cggatcgctg agatttccca tgacatcgat gtggaaaaag tattcaaaag
     1681 gcctcccttt aaggggccgt gactccaagt gagtcatatt cataccgttg tagaaaaagt
     1741 agcccaagac gtgatacaag gcaccggggc gatgctgcgt cgtcaggacc aaagtgattt
     1801 tatctgcctc cggcacttct tcccggtcag ccgtaatgat gaaaaaacga gtatagttct
     1861 gggtattgtc gttgattgat tctgccag
//
LOCUS       DAWDXZ010000375         1887 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10754_length_1887_cov_5.073144, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000375 DAWDXZ010000000
VERSION     DAWDXZ010000375.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10754_length_1887_cov_5.073144"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    776..850
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1075..1084
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          491 a          424 c          368 g          519 t
ORIGIN      
        1 tcagccttat ttcaatggtt cgaccggtgc cgccgccatg gtttttgccg gaagctcggc
       61 aagagatttc tgagccgccg ccgcgtcgat gcgggcactt tcctgttcgg ccacatcgat
      121 gcccagacgc agtttcgctt ccttcttaat ggccttatag agcccgttag gaaaatcggc
      181 aacggccgtc gttacgccgc ggggaaagac ggcttcccgt tcgagggtat agccttcagg
      241 atagagacgg gagcagcggg gatgatacat gtcgacgtac agccggtcgg caaagtgatg
      301 gatgcgcccg atctgtccga cattatgaaa aaagaccatc gatcctgtcc gccgcaggcg
      361 caggcgcgac aagtagacga actctccact cggcccgttg acgggaatgg gattggtcac
      421 atcatggcca aatcggccgt aagccccgct cacctgatca cagtcttcgc cggcaagtac
      481 ttctcgggca agttccgctg acaaggcctc gttttgcagc tgaggtgaat taaagatctg
      541 cagggcacgg tcataaatct cttcgctctt cgtgcgctgc tgctctttaa aaaacgcgtc
      601 gaaaaatccc atcatattcc tcctaatatt atcatacaat gggccgcagt cttaacggta
      661 tataggggag actgccgccc ttttctttat tatattcatt atagaacatt ctttacgctc
      721 ctgccaatac ttatctcacc attatcttat tttatttttt taagtcacac ctttannnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn ttcttgagaa atgattattt tgcctaatac gcgaaaatat ttttccgctt
      901 ttcgcttgat attctctatt ttatgcgctt atttttgcat tcaaagcata ttgaccccct
      961 acaattgcat ttttcagcta ttgattttat tttaatcacg cgttattata tttatagata
     1021 aagagatcaa acaccagccg gtcattatcc agtaacaact taaaatatta ggctnnnnnn
     1081 nnnnttataa agaagaaatg agggagaatc atgaacttaa gtgcaaacgc aaaaatcatt
     1141 atttccatgg gtgctttgct ttcgtacagc ttcattgcac accttcttta atatgtccga
     1201 ctgtaatgcc tgaaaaagcg acctcacgta ttgtggagtc gcttttttag ttgccatttt
     1261 cagttctatc atcaccattt gagctttgta cccattccgg tctccgtccc cccttgccat
     1321 atataaagaa taatatatta ctaactatca ctaacacagc caaagaacgt ccccatcacg
     1381 aagagtctta taaagattat ataaataaca tatcagctga tggaatgcag gagagaggca
     1441 agtaagagac aacgaccaac aaaaaggccc tgtgtgaacc atacccgtaa catggttcat
     1501 acagggcctt taaaattcta tcgggcattc agagcgtcaa gccaatcaat acgatacaga
     1561 caatcacaac gatcagatct cttacggtct gcttatattc acgatgattg tagtaagaca
     1621 cagcggcatg gaagattgtc ccgaacatta catagctcac taagatacgt atgttctcga
     1681 tgaaggttcg cggatcaatt atatttatca gtccaatgct tataatgcca aaaagtaacc
     1741 atgctatcac gtgtgcatct acctgccttt caaagttaat atgtgtcatc aagtctctat
     1801 ataactatac agacgttttg atgtgctgtc taggtgtggc gctgtgattg taatggctag
     1861 tacaatttag cgcaggcctt gatagtg
//
LOCUS       DAWDXZ010000376         1882 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10778_length_1882_cov_6.975917, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000376 DAWDXZ010000000
VERSION     DAWDXZ010000376.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10778_length_1882_cov_6.975917"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1029..1038
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          477 a          508 c          432 g          455 t
ORIGIN      
        1 gtatgagccg ccgcgtcgcc cagcccataa tggccatgcc gacgataatg acaaagatac
       61 ccaaactagc caaaatcgta taattatagt gccccgtcat ttcatagagc aaccggaaag
      121 cccactcttc cacttccccc aaccactgat agttaaaagt caggataatg ccgaagctgg
      181 aaaacatcat gcccagcccg aaaaggaaga gccagcgctt aatatgcagt cccggatgca
      241 accatttcat aaaacgcatg acagtacctc ctaggcttcg ccttcgtacc ccggcgcatc
      301 ttcttcaacg tcgtttttct gaatatcgcg gtgttcgatg gatacatcga cgccgttgtc
      361 ccgcaagtgt ttgtacatgg cttctgccat gcagacactg cggtgcatcc cgccggtaca
      421 gccgacggca atgactaatt gactcttgcc ttctcgttcg taattaggca ggagaaaatc
      481 aatcatatcg aaccattttt ccttatatac ttgggtaata ggaaaacttt caacgtactc
      541 ccgcacgcag ggaacgcggc ccgacttatg gcggaaatcg tcaatataat aagggttcgg
      601 caggaagcga acgtcttcaa cgatatcggc atcaatgggc atcccatgct tgaagccgaa
      661 agagacgacg gtgacagtca tactcttttt gctgtctgta atagcatagc gcttcaccag
      721 gatttccttc agctgtttcg gctgcaaatt agtcgtatcg ataataaaat cggcctttct
      781 ccgcaaagaa gccaggcgtt ttcgttcttc ggcaatcccc tgactgatgc ggccgtgagg
      841 ggccaacggg tgaacgcggc gagtttcttt atagcggcgg accaaagcct gatcagaagc
      901 ttcaataaag acgatttcat aagaaattcc ttcttctttc aaatccttca gatccttcag
      961 gcactgactc atggcatcaa aaaattcacc gccgcgaata tcggcaatga gggcaacatg
     1021 gcgcacccnn nnnnnnnnaa atttctcgac gacagcggct acgccgtctt catcattcga
     1081 caaggtgacg tagtcagctg catccttgac ttcctgtccc gcattagcca tggccacgga
     1141 aaaaccggcc gctttcagca tgtccaaatc gttttgcgaa tcgccgatgg ccatgatgtt
     1201 tttcggctca atctgataga tctcaccaag gcggcggata ccacggccct tatcgacgcc
     1261 aaggggcatg atttccaaaa aagtcaaatc ggaaaacagg atattaaatc ggccgggaaa
     1321 agtcttttca ataagagcct tgcggcctaa gagctcttca tgtacaccgc ggctgaggag
     1381 tttattgcag tcaccctgga cccgataaaa ttcatccccg cagatgacgg ccttgacttt
     1441 ggtaattcgt tcatagtcac gaacccacgg ttcatcgagt tcaacccgca gtacgtcgtc
     1501 aatataggtc tgcatctgcc aggactgttc ccgtgccaag ttgaccagcg cctgggcatc
     1561 ttgctgagaa atagtctgct gaaagaggat tttctttgat tccaaggttt caatcagacc
     1621 gccggcaaag agcagcatcg ggctgtcgcc taaagccagc cgttcgccat aagcctttgc
     1681 cgtctggtac atacggcccg tagcaatggc gacgatgacg cccttctgac gggctttttg
     1741 cagcgttttt acggtgtagt ccgaaacgga accgtcactg cgaaggagtg tcccgtcgag
     1801 gtcgattgta atcatcttaa tgggttccat tatttattag ctcccttcca gtcaaaagcg
     1861 acacctttcg gcgcatcgtc cg
//
LOCUS       DAWDXZ010000377         1859 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10924_length_1859_cov_6.903548, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000377 DAWDXZ010000000
VERSION     DAWDXZ010000377.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10924_length_1859_cov_6.903548"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          400 a          535 c          431 g          493 t
ORIGIN      
        1 catatcgatg agtttatccc attcagcatt gactcgctcc atatattttt caagccggat
       61 atatttatca gcagtatcgg aatttttcgt accgctaacg tgctcggaaa gactgctgca
      121 tcgtagggat agtatgtcag ttctgatttc ggccaattcg tattctgtct gctttagtac
      181 atgattttga ttccgaatcc ggttaagata ttccttagct gtcatactac ctcctaatta
      241 ccaactcaat aatcttctgc ccgcgcttgg tgcacgctac ccggtatccg gcttgggcgc
      301 acaccagctt gatgatgcgg agtaccaact ccgtcttata gtcgtccttt cgtatgttgt
      361 ccagcgcctg ccctgccgta gggtcccgat acccttcact atttttcata ggctcatcct
      421 ccttcccgga ctgcgttgat tctggccatc aggctattca atacataagc ctgtacctcg
      481 tcctttttgt cgagggcctc ggctaagtcc tcgtcacgtg tacccacaca tatgagttgg
      541 tgcacgataa cgggatgctc ctgcccctgc cgatgcagcc gcttgttcgc ctgttggtac
      601 aattccaagg accagttaag gccaaaccat atgatgtggt gcccgccttg ctgtaagttg
      661 aggccgtagc cggtcgatgc cggatgcgcc agtaagatgt cgacctgccc atcgttccaa
      721 gcctgctcat cgtccgcatt tttgtagata cggcatcgga gtttcgtctt tttcagcgcc
      781 gtcatcagcc gctcccggtc atgctggaag ttatagaaca ccaaagcatg ctgcccgtta
      841 agtttctcga ccaactccaa aaacgcctct atcttgcagt cgtgtatctc atgcgcctcg
      901 tggtcgccat cgtagatagc cccattggcc agctgctgca acttgttgga taacgccgcc
      961 gcggatgtga cgtcgatatc tccttccggc aattccagaa tcatggtacg ttccagctct
     1021 ctatatgcct tcttggcttt ggggctaagg actaccggca catcgtcata gatgcattcc
     1081 ggcagctgta agtaatccga cgccttcata ctgatacaga tatcatcaat ccgactcatg
     1141 acggcatcct gtgccccgtc tttggggtcg tactcgtaga tgacatcgcg actacggcgc
     1201 ccaggctcaa aatatcgctc cctgaaatgc gtgtaatatc gccctagccg tacgccacga
     1261 tccagcaagt acacctgact ccacaggtcc tctaaaccat tcggggaagg tgtcccggtt
     1321 agccccacga tacgcttgat gtggggccgg atagccgata aggccttgaa acgttttgcc
     1381 cggtggcttt taaacgatga aaattcatcg caaaccacca tgtcaaacgg ccaatcgttc
     1441 ttgtagtaat cgactagcca gacgacgttc tcgcgattga caacgtagat atccgccggc
     1501 gtattcaggg cccggatgcg cttggactcg ctccctagca ccgtcgaaaa ccgcagatgc
     1561 ttgagattat cccatttagc ggcttcgcgc tgccatgtgg cttctgcaac ctttttcggg
     1621 gcgataacaa ggacgcgctg aacctcgaag cggccatact taagctcact gatggccgat
     1681 aacgtgattg atgtcttacc aaggcccatg tccagaaata gcccgatagc cggcttttgc
     1741 aggacctggt caatgcaata ctgctgatat ggatgcggct tgaatatcat gatatgcgcc
     1801 ccccattaat ctaattccag ctcccgtttc acgcctacgc ccagccgcag ggcgtggac
//
LOCUS       DAWDXZ010000378         1857 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10930_length_1857_cov_8.646504, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000378 DAWDXZ010000000
VERSION     DAWDXZ010000378.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10930_length_1857_cov_8.646504"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          524 a          453 c          505 g          375 t
ORIGIN      
        1 atcgtttgcc ctcatcgaga tggccattgc cattgcagag ggccgtaagt ggctcaattg
       61 gtattgctct aagggccgcg tcctatacgt caatcttgag cttgaccggc ctagctgcct
      121 gcaccgattt aaggacgtct atgacgcctt gggatacccg gccaacaata ttgcggatat
      181 cgatatatgg gagcttcgcg gcaagtccat ccccatggac aagttagccc cgaaactcat
      241 ccggcgggcc gccaaaaaaa actacacggc cattatcatc gacccgatct acaagatcat
      301 cacgggcgac gaaaacagtg ccgaccagat ggcccatttc tgcaaccagt ttgacagggt
      361 atgtactgag cttggctgcg ccgtcatcta ctgccatcat catagtaagg gcaaccaggg
      421 cgccaagcga tccatggacc gggccagcgg ctccggtgta tttgcccgcg acgccgatgc
      481 actactcgac ctcatcgagc tcgacactga gggcgccgac gacattgccg gcacggcctg
      541 gcggatagaa ggcacgctgc gggagttcaa ggccttccag ccgatcaatg tactatttaa
      601 ctatccggtg cacgaggtcg acaccgaggg gaaactagca gcctatagcc ctcacgcaga
      661 caaggccccc tggcaaaagg ggcaagcgac tcaaaaaaaa cggaaagaag ccaaaataga
      721 gcagcttgaa aatgcttata atgcgctcat gattaacggt caggtaactg taaaagatat
      781 ggccgaatac atggaagtct ccgaaaggac cattggaagg tacgtcgaaa aatctaaaaa
      841 atttgaggtt gataaaggaa ttgtaataat ggcggcggcg gcatccgaac ggacgccaaa
      901 tgataaataa atgaataatg acgcattaaa ataccgacaa tggtacggat gccgttatat
      961 atatacgcac tttcacggcg gcatccgtta tatatatata gatgccgccg tctgtatatt
     1021 gccgtcgggt ccttggacaa caaatagggg tacaaggggg cctaaagtgc gcccttgtcc
     1081 ctattcccta tttgtcgtcc tgaaacagga ccatgcggga gtgggaaaag agaaaaaaag
     1141 aaaaaataaa acttgaatat tttgacgagg tggtaatgat gattcatatt ttcctgccga
     1201 tggagaagct gccgtcggcg acgcaccaga tgaaaaaggt tacggtacgg aatggtaagc
     1261 cggtagtata tgagccgccg gcagtcaaag aggtccgggc gatgtttatg gcccattttg
     1321 cgaaataccg gccagagcat ccgatggaag gccctatatg gttggtgacg aaatggatat
     1381 acccgccaac aactaaacat cctgaaaata cctggaagac aaccaagccc gatacggata
     1441 atctcgtcaa gctgcttaag gacgtattga ctgacctggg gttctggctg gacgatgccc
     1501 aggtggcgtc ggaggccatt cagaaatttt acgcgccaac acctgggata tacgtccaga
     1561 tcgaggaaat gattaacatg taggaggtga gtaatatggg acggcaggag atgatagagc
     1621 gggcacggtc ggcgttccga gaggtacttg aggcgatggg aaagcctaac gcgcagctgc
     1681 ttcagcggga cccggatatc aagggcctcg tcgaaaatat cgtccaacac gtggaaaatg
     1741 cccggaagcc cgaaaactgg ccggtccttg agtatacaga cgacttccgg caataccacc
     1801 ccgaagaccg atggcaatgg gcctggctat ttctggaggc cgctatcatc gacgagg
//
LOCUS       DAWDXZ010000379         1855 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10947_length_1855_cov_7.704444, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000379 DAWDXZ010000000
VERSION     DAWDXZ010000379.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10947_length_1855_cov_7.704444"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    301..310
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1025..1034
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          469 a          491 c          429 g          446 t
ORIGIN      
        1 ccgaatacgc ctttgggcga ataggacatt ttatggcaaa ttacgcctac tctgtttcca
       61 tcgtaattgc aaacgtcgcc attgccatca cggcagtcgg ttacggcgcc ggtttccttg
      121 agacaacctt tacgccgatt cagacgtgtc tatatacgat cgctttattg tggattgcag
      181 ctgccctcaa cttcagcggt tcccggtatt cgggcaagct gagtgccatc accatctggg
      241 gcgctattat tccggttctc ggtatttcta tcatcggctg gcactggttc agcccggcaa
      301 nnnnnnnnnn tcagcgtcgc tgtcatctac gtcgcttcga cgaatgtcat cgccggaatc
      361 gttcccaatg ccgaaatcct ggcatcgagc gcacctttcg gactggcctt ttcctacatg
      421 ctcggcgata cggccggcaa ggtagtcatg ggcctgatgg tattctcctg cgcaggctct
      481 ctcctgtcgt ggcagttcac cctggcccgc gtcttcaaga ccagcgccca gcgcggcttg
      541 ttccccaaga tttttgccaa agtcaccagc gccgatgtcc ccgtcaaggg catgctctgc
      601 atcttaggca tgcaaagcgt cctcgctttg atgactatca gcccgacctt ggccgcccag
      661 ttcgaactct tggcgaacct ggccgtcgtc accaacgtca tcccctacat cctttgcgcc
      721 ggggccttga cgacaatcct tactaaggaa aacattccca ataaagtctg caatcgaaat
      781 acttcgattt tcttagcagg cgtatcgatt atctactgcg tatacgcatt gacaacgacc
      841 gatgcgacga ccttcctcag cggcagtttc gccgccttcc tgggctggct cgtctacgtc
      901 gtccgcttca cgatcatgaa gcagaccatc gtcgaaatta aatgaacaga tcaaaaagga
      961 gaccccttca ggaggcctcc tttttttaca gcctttccaa aaaaccgacc gtaaacagaa
     1021 ccacnnnnnn nnnnacggca gcagccctgc tcgattatgg aaaggcttac ctatcagata
     1081 ggagtattca ctatggacat gaccttaaca ttatgtaccg tcgatgactt ggatgaactt
     1141 cgtaaattat cgattcaaac gtattacgaa acctttgcag ccgtgaatac ggcgaaaaat
     1201 atggaagaat atttgaaccg ggcctttgac cgggaaaaac ttcgccggga attggaaaat
     1261 attcattcaa agttcttcat cctgcgctgc cacgaaaagg cagccgctta cctgaaggta
     1321 aacgaagcac cgtcacagac ggatatcaat gacccggagt ccttagagat tgaaaggatc
     1381 tacgtatccg gagaatttca agaacaaggc ctgggacgct atttgatgga acaagccatt
     1441 gccatggccg tggcgcaaaa gaaaagatac gtctggctcg gcgtttggga acataatgaa
     1501 aaagcccttc aattctacaa ggccaatgga ttttataaaa tcagcaccca ccactttgtc
     1561 atgggagatg atgtgcagac agacgatctc atgagaaaag atttagacca agcccactaa
     1621 gaatccgtca aggcgtcgta atcgattctt gggccgcaaa aagccctcat gatcaaagga
     1681 acgtttgctc atgagggtta tctatattta taagggcgct gcgacgactc atgtcccacc
     1741 gggtcgtcac actgtaagga tttttaggtt ggaaaacatc ttatggagcc gataggcctc
     1801 aaagggaagg tattcggcaa tggtaaaacc gacaatatcg gagcagccct ggatc
//
LOCUS       DAWDXZ010000380         1854 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10957_length_1854_cov_4.828238, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000380 DAWDXZ010000000
VERSION     DAWDXZ010000380.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10957_length_1854_cov_4.828238"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    907..921
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1493..1502
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          465 a          439 c          402 g          523 t
ORIGIN      
        1 atgtaatatt ttaatactat tttaatattc attgatattt attatcgttt attgtagtat
       61 atcatgaaat ggccgcattt gtccaatagt ttttttatta acaataagtt tattttttat
      121 aatattaagt acaaacttta aacaataaag tacgtatagc ttatacctgc tttatgaaaa
      181 taaattctca aaaattagag ggttgttcaa taaccttgcc cggattcaat atccacttag
      241 gatctacggc gcttttcaag gtcttcatca ggtcgagttc agccggatcc gtatattctt
      301 ccatatactt gagccgcttc aaaccgattc cgtgttcacc ggacagacgg cctcccagat
      361 tatatacata agtatacgcc gtcgaccgga agtccttcaa ttgctgttcc caagcttcat
      421 cgctcatgtc acattttaaa atctggaaat gcaggttgcc gtcaccggcg tgggccaggc
      481 agaaaatacg aaagtcgtat tttttacttt cgttgaccaa atgctgaatg cagtcagcaa
      541 tcttaggaac gggaacgact aaatcttccg acgtagcgac ttttgaaaaa gcccttgtgc
      601 tttccagaca gttgcggcgg accttccata cccgatcgtc ggcttcgacg atatctgtcg
      661 ctccgcattc ttcacagacg gccgccagtt tttccatctt gtcgtccaat tcatcttcgt
      721 tgaaggtttc aatggaaatg atgtcataac agccgtcttc gtaatggggc agcttcaagt
      781 cactgtaatc gctggcgctg cggacgaaat tgttgtccat gaattcgacc gacgtcggat
      841 tgaggccggc cttaatgagt ttcgggacca gggctgtcgc cttggcaagg tcggtaaaga
      901 cggccannnn nnnnnnnnnn ntcgctttgg taatgatgcc cagcgtccct tcggaaccga
      961 tgatcagctg atccaaacag aatcccgtag aacatttctg aagattcgcc ccaaggttta
     1021 cgattttccc cgtcggcaag acggcttcga tggcatagac ctggtggcgg gtcgtaccgt
     1081 aacggacggc cttattgccg ccggcattgg ttgccaagtt gccgccaatg aggcagctgt
     1141 cggaactgca ggggtcaccg gcgtagagca gtcccttttc gcgggctgca ttttgaatgt
     1201 caatcgtccg aacgccggct tcaacagtca tatacatgcc gtcttcgtcc agctcaagga
     1261 ttctattcat ccgttccatg agcaggacga tgccttcata ggccggaacg gcaccgcagc
     1321 tgacgctggt accggcactg cgcggcgtga ccgggacgcc aaatcggttg gcaatctgaa
     1381 ggacctcaga aacctcttcg gtattcgccg gcaagacgac gacttccggt gtcctccaaa
     1441 aagacggcgc caggctttca tccgatttat acacgtcgag gatggccgga tcnnnnnnnn
     1501 nnacggcatt ttttaaagct tctaacactt catccgtaac cggattatat ttttccatcg
     1561 taaaatcccc tttgctgttt cgcagaaatg gaactgaggt ttcgcccagc gcaactgctt
     1621 tttttaattt tatgactatt atagtacagg cgcccctatt tgtctctaaa atttcaaaaa
     1681 aaggatatat ccctttatca ttttatttat atcttcccta tatcttaaag aacggttaaa
     1741 tccatgggtt ttatataaaa aaaagaagct gtctaaaaag acagcttgct gtatctatcg
     1801 tgcgcgacgg cagatgctga aaaagattcc ttatagtacg ggaaaggtcg ctgc
//
LOCUS       DAWDXZ010000381         1848 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_10989_length_1848_cov_8.161182, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000381 DAWDXZ010000000
VERSION     DAWDXZ010000381.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10989_length_1848_cov_8.161182"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1323..1332
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          475 a          529 c          390 g          444 t
ORIGIN      
        1 catctccatc ccatgatgct tctcctccca aattgtattg tgccatggaa tctcccagtt
       61 caacggcttc ccgataccat ttctgatgcc atattgcacc gccccagtcc cgctggttct
      121 catacacgct gcggaaaacc tccccgacat cgtctcgcca caatacaaat tcagcaacca
      181 cctcgaccgt atggagcttc agaaaaaggg tgagctgctt tggcgaacga gaaatctttt
      241 ttaccatatc acggttacag ggaagatgaa cccgagaatt gacaaccccg gtcttagtca
      301 atcctacatc tttcaaaatc gtttctgcat aatcatcttt tgcacaaaag caaaagggcc
      361 gaataaaaaa cggcagcctc tccgcctcgg catcatagtc tgcaagtacc gccgtcaccc
      421 taccaaaggg caggaagggc atctgacgga ttctttcttc ataagcgacc tccgtttcat
      481 acgggaatgg ctcccaacag atgggctgta cagcgatcaa ctcgtcgcca tgcttcagat
      541 acaccttact gtaatcacaa cagacctcat cctcatgaat ccttaacttt gccaccaact
      601 ccccgtcgat gaccgtgtca tcggtcacgc cggtcacgta aaaagcttgg acaggcgaaa
      661 atcggatatc cggattacga tcgatatggt acctcaaaaa acagctgtcc gtatacccgt
      721 cgcctttacg gggatcaaga acagcacagc caatatacac aggctccata gccgtaatcc
      781 gctgccggaa ggcactcggc gtttcaaagc cccggcgaga caggggctgc acctgctgta
      841 cctctttttc ggtgtccccg gacagtcttc caccttgagt caaatagcgg ctcacggcgg
      901 ggacatcagc gggccaaaag tcttccgtcc tgtaggattt tcccttatac agcccatagg
      961 ctgtggtcag gatcaataac cgttccaaac acagttgcac atccccatcc tcccggccat
     1021 aagactcgac catacgacga acctcatgga aatggcggct taagccttga tccacgagct
     1081 ttttttcttc taattcattg atgccctcaa acaaattcaa ataggcagcc cctaaatcac
     1141 gaatcatcca tctcatagcc aggctcatct cctccatggc ctccttagcc tcggaagcct
     1201 cctcggccca gacgcagaga tcatacaatt gaggaaaatc ctgctccaaa aaacaaaatc
     1261 tcgataccgt catctccctt acctcccaat agtgtgatgt ctaattttca tcggtgctgt
     1321 aannnnnnnn nntcgcgcag tcatgacaga atatgtcatc gtccgcaata atgatgttgg
     1381 ccttatacaa atttttcctg ttgcccagtc aaacccatcc tatacaaaaa aagactcgcc
     1441 cattgaaaaa ccgacatcct aaagttagat ttttaggtcc aactttaagg gggggcggtt
     1501 cacaaaaggc aagtcccttt tacgctccag ccgccattta gaaatggcaa taggctaggt
     1561 ttcattcata gccgtattcg gctgttatct atcttgattt aagccgcacc gttttcgacg
     1621 gttgtcttat cggatacgcc ggaaagtgat tcatattctg ccgtcgggaa accgtaccag
     1681 atcacgtcat ggttatcgtg tgggctgatg gcgatatggg caaaggtact gtccggggca
     1741 gcgccgtgcc aatggacgac accgggcggg cagtaaacga cgtctccggg atgaaggact
     1801 tctaccttac cgccttgtat ttgatggtag ccgacgccgt cggtggcg
//
LOCUS       DAWDXZ010000382         1845 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11007_length_1845_cov_7.813408, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000382 DAWDXZ010000000
VERSION     DAWDXZ010000382.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11007_length_1845_cov_7.813408"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    813..822
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          397 a          473 c          473 g          492 t
ORIGIN      
        1 atgtattatg cgggagactg gattataggt tatttttatg gcttaaatag cctttaacta
       61 tataattgtt aagtgaatat atattataca aagaaaataa tatgatgtac aataaagaaa
      121 agatcgtgag agagctcctc ctgccgggcc atagctttca agaagcgcgg gttaaaacaa
      181 aacggttcgt gcgtggacgg ctgcccgtcc gttacccttg tgatacggcg ccactacagg
      241 ggacgatggg acggtgtgaa acgatactct ctaagatgcg aaaggagacg acttatgaaa
      301 gataatgaag gcctcgaaaa gaaggtggaa aaggccatta ccaaggaacg gcgttatgaa
      361 gaccacccta taaagccaaa cgcattggct ttgattccct ttgtcgtatt cattgcagtc
      421 tacttgggca gcggtattat tctcgatatg agcggcatgg aaatggcatt ctaccaattc
      481 ccggccccgc tggcggcggc tatcggtgcg attacggcat ttatccttat ccgggacagt
      541 aactttgacg aaaaatttga aacctttgtc aaaggatgcg gcgattccaa catcattatc
      601 atgtgcatca tttacctcat ggccggaggc ttcgcggcta cctgcaaggc catgggcggc
      661 atcgactcga cggtcaattt gggcttgacc tatattccgc cggaatacct ggcggccggt
      721 gtctttgtca tcgccagctt catcgccacg gcgacgggga cctccgtcgg cagtgccgct
      781 gccatcggtc cgattgccgt agctgtcgcc gannnnnnnn nnccgaaaag gccggcatcc
      841 ccctgccgct gatgatcggc tgcgtcatgg gcggcatcat gctcggtgac aatctctcta
      901 tcatttccga tacgaccatc gcttcaacgc ggacgcaagg ctgcgccatg aaagataagt
      961 tccgccttaa tttttggctg accctggtgc cggccatctt gaccgttatc ttgctcgtgc
     1021 tctttggcgg ttcagagacg gccgtcagcg ccggtgatca tcccttcagc ctgatcaaag
     1081 tgctgccgta tctcgtcgtt ttggtgacgg ctgttgccgg ggccaatgtg tttgctgtcc
     1141 tcgtcggcgg cacgctcctg gcaggggcta tcggtctctt ttacggagat ttgtcgcccc
     1201 tgtcttttgc ccaagcgatt tacaaaggtt ttttaggcat gacggatatc ttcttcctgt
     1261 ccatgctgac cggcggcttg gcggccatgg tcaacaaggc cggcggcatc gcttatctct
     1321 tgaccacggt ccagcgcttt atcaaagaca agaattctgc cgaattgggt atttcggcgc
     1381 tggtttccat taccgatatt gctacggcca ataatacggt ttctatcatc atcaacggcg
     1441 aagtagccaa gcgcatttgc tataagttcc acgtcgatcc ccgccgcagt gcggccctct
     1501 tgagtacctt ctcgacgatt ttccagggcc tcgttcccta tggggcccag atgctgatca
     1561 tggtcggctt tgccaacggc aaggtatcgc cgctggaagt cctgccgtac tgctggttta
     1621 tttaccttct cggcatttcg gccatcattt cgatctttat tcccttctca gacggtttta
     1681 ttcgcaagga tccttggaat tttgaagctc aaaagcccca gagtcaggtt gacggctcgc
     1741 atgaaaaggc tgcgctcttg tagcggctga gccgtgaaca taaaaaactc ttccccggtg
     1801 catggggaag agtttttttt gcctttattc tgaccagtgt ttcgt
//
LOCUS       DAWDXZ010000383         1842 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11025_length_1842_cov_7.028539, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000383 DAWDXZ010000000
VERSION     DAWDXZ010000383.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11025_length_1842_cov_7.028539"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    719..728
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          454 a          467 c          500 g          411 t
ORIGIN      
        1 gaggctgccg gctttgctga tgacctgcga aatcaggctg agatcgctga ctttgacggt
       61 caggctgttg gttaccgtat aggaggtgac cttagagcct tttacatcat agttgggcga
      121 catgtagaag ccggtggttt tgaggttgtc tgccgggatg cccatcgctt tgatggcgtt
      181 ggtgacgttg ttcatgactt gattgttctt ggtacgagcc tgctgagcgt cggcatcggt
      241 gctggatgta ccgatggtaa tatatgccgt gtcaggagcg acttcctgct gggcatagcc
      301 ggtgacggtg acgacggacg gctgggggcc ttcagcgaaa gcctgagggg cagtaacaaa
      361 gagagaggat gcgagcatag cggccacgac gaggccttta aagacatttt tttcatgtaa
      421 ataacctcct ttgtaatgat tgtatcgatt atatcatatc tgacagaaca tatccactcc
      481 caaaagaaaa tcaggacatt tgtctactta gaatgttaca gaagtattgc attttttggc
      541 cgggcatact ataatatttt aagatacata aaagaagcta ggggagcctt cgggctgaga
      601 aaagtgctgg attacacttg accctttacc tgatgcggat aatgccgccg gagggaagct
      661 ttacgggatt ataaagctca cctgcggagg ccgcgcggtg ggcttttttt gtggcaatnn
      721 nnnnnnnngg aggattttat aatggattac acaacacaaa tggatgcggc ccgcaaggga
      781 atcgtcacgc cgcagatgaa ggtcgtcgct gaaaaggaaa agatggctat tgaaaaactg
      841 atggccctcg tcgccagcgg caaggtcgcc attccggcca ataagatgca tgaaagcctg
      901 tcggctgaag ccgtcggcca ggactgccgc accaagatca acgtcaactt aggcgtttcg
      961 ccggaacaca acaaccatga agaagaactg cagaaggtgc agaacgccat cgatatgaag
     1021 gccgaagcca tcatggactt gtcgaacttc ggtaagactc gggacttccg ccgcaaatta
     1081 gtcaaaatgt cgacggccat gatcgggacc gttcccatgt atgacgctgt cggcatgctc
     1141 gataaagacc tgaaagacat cagcgtcgac gaattctttt ctgttgtcga acagcacgcc
     1201 gaagacggcg tcgacttcat gaccatccac tgcggcatga accgcaagac ggcacagcgc
     1261 atcaaagaca atccgcgcct gactaacatc gtctcccgcg gcggctcgct cctctttgcc
     1321 tggatggaaa tgaacgacca ggaaaatcct ttctatgaat attacgatcg cctcctcgac
     1381 atctgtgaaa aatatgacgt caccttgagc cttggcgatg cctgccgtcc cggctgtacc
     1441 cacgatgcga ccgatgccgc tcagatcgaa gaactcatta ccctcggcga actgacgaaa
     1501 cgggcttggg accacaatgt ccaggtcatg atcgaaggcc cgggccatat ggtgctcaac
     1561 gaaatcgctg ctaacatgaa gcttgaaaaa agactctgcc acggggctcc gttctacgtc
     1621 ctcgggcctt tggttaccga tatcgctccg ggctatgacc atattaccag tgccatcggc
     1681 ggtgccattg cggcatcgag cggtgccgac ttcctgtgct acgttacgcc ggcagaacac
     1741 ctgcgcctgc cgaccgtcga agacgttaag gaaggtatca tcgctgcccg cattgcagct
     1801 cacgccgccg acctcgtcaa aggtattccc ggcgcccagg aa
//
LOCUS       DAWDXZ010000384         1840 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11038_length_1840_cov_9.952941, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000384 DAWDXZ010000000
VERSION     DAWDXZ010000384.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11038_length_1840_cov_9.952941"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          449 a          457 c          524 g          410 t
ORIGIN      
        1 gctggggctt gaaagcattg aagacttccg tcgccgcggc ggcaacgtgg aatttggagc
       61 ctttggcgaa aatctggtcg ttgacggctt tatcttttca aaacttccgg taggaaccct
      121 gcttcgctgc ggggatgtct tgctggaaat cactcaaatc ggcaaggcct gccacagtca
      181 ctgtgcggta tatgaagccg ttggcgactg tattatgccg cgggaaggtg tctttgctaa
      241 agtcctggag ggcggtgtca tccatccggg agacgagatg gtagaagtag aacgcgggga
      301 aaaacgaccg taccaggcag cggttattac tctttccgat aagggctctc agggactgcg
      361 ccaagacgaa agcggtccgg tcatcgttaa aaggttggaa caggaaggct atgacgtcgt
      421 cgaaacgatt ctcctccccg atggacggga cgaattggag caacatctgc gccaactggc
      481 agaccagcgt caagtcgatc tcattttgac gacgggcggt accggttttg gccagcgcga
      541 cgttaccccg gaagcgacca ttgccgttgc cgaccgtcag gtgccgggga ttgccgaggc
      601 catccgagcg gcgtcgatga aggtcacaca gagggccatg ctgtcccggg ccgtcagtgt
      661 gatccgtaag aaaaccttga tcatcaatct gcccggcagt cctaaggctt gccaagagtg
      721 catggatgtc tttttagaga ccattcctca cgggttggat ttacttcgcg gcggtgtttc
      781 tgactgcgct cgcaagtaaa tttacataaa tgccattttt ataaaggaga tgcatgacca
      841 tgatatggaa aaaagcagca gcggcggtgt tagctgggat gatggctttc gtcttggtcg
      901 gctgcggagc ggaaaaacaa gcagccccgg gtgccggcaa tggtgaaaaa gttcatttga
      961 cggtatttgc ggcagccagc atgacggaaa cgatgaccca aattgccgaa caatatcaga
     1021 aagagcatcc gaatgtcgaa atttccttta actttgattc gtcgggaacg ttgaaaacgc
     1081 agattcaaaa cggcgccggc tgcgacttgt tcatttcagc agcgcagaag cagatgaatc
     1141 agctcgatgc cgccaaagac ggcaaggata atcccgataa actcgatttc atcaaaagcg
     1201 acagccgcat cgacctcttg gaaaataagg tcgtcttggt cacgccggaa ggaaatccca
     1261 agggcattca gaatttcaat gacatggccg cgaaattaag agacggttcc atccggctgg
     1321 ttatgggcaa cagcgacgtt cctgtcggtc agtatacgca gaaaatcttg aaattctaca
     1381 acctcgatga accggctatt gccgaggccg gtcacattac ctacggttcc aacgtcaagg
     1441 aggtcacgac tcaagtccgg gaaggcagtg ccgatgccgg tgtcatctat gcgacggacg
     1501 ctttcagtgc taatttgaag ccccttgacg aagcgacgaa agagatgtgc ggccaggtca
     1561 tctatccggc agcggtcatg aagaacagcc gacatccgca ggaggcacag gatttcttga
     1621 attatctcaa gggtgccgaa gcgatgaaaa tcttccagcg cgtcgggttc agccctgtcg
     1681 gctaaaggtg gtcgactatg gattggtatc ccctttggaa cagcctgcgc attgcggcta
     1741 tcgcctgcgt catcgtcttt ttcagcggca tcgccttggc ttactatgta gcacggctgc
     1801 cgcgggtctt aaaaggaatt ctcgacgtcg tcctgaccct
//
LOCUS       DAWDXZ010000385         1835 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11072_length_1835_cov_6.758989, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000385 DAWDXZ010000000
VERSION     DAWDXZ010000385.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11072_length_1835_cov_6.758989"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          449 a          463 c          421 g          502 t
ORIGIN      
        1 tggagctcac ggttttctcc attgacagta acacgcatgg tgtacacctc cgcttgagtt
       61 taatcaaagt aaatacaagt tctattatac aggattttaa ggccgcgtca aagggatttt
      121 tgcaaacaaa aaagaaccga cacagtcggc ctgagcctca ctgcggcagt tcttttcttc
      181 acgtatttac gcttctacag ggtatacgct gactttgcgg ttgtatttac cagcgcgttc
      241 gaacgcaact ttgcctgcag ccgtagcaaa taacgtatca tctttgccga tgcctacgtt
      301 agtgcccgga tggaagtgcg taccacgctg gcgaacgatg atgttgccgg ctttaacgat
      361 gacgccgtca tggcatttta cgccaaggcg tttcgattcg ctgtcacgac cgttacgcgt
      421 ggagcctgtc ccttttttat gagcgaaaag ctgtaagttg aaattgaaca ttccgtccac
      481 ctcctgacat ctataagtcg agtacgacgt attctccata ctgtcgcacc atttcctgca
      541 atcctcttgc catagtccct aagataatct ctgacgcttc agagggctca ggaaccgtta
      601 ccgataagaa tccttcgttt acttcatagg gtacgtcctt cgaagcatac tccatgagac
      661 ccaacaaagc agtctgtgac agtgccgata cggcggcaca gacaatatcg cttccttctt
      721 ccgcataatc ggcatggccg cttatggtaa atcccgtata gcgatattcc ttatctcgat
      781 gcatcgtaat ggtaatcatt agccttcgat tttttcgata gaaactttcg tgaaaggctg
      841 acgatggccc tgacgacgac ggtagttaga ttttgctttg tatttgaaaa caaggatttt
      901 cttttcttta ccctgagcca atacttttgc cgttacttta gcgccttcga caacgggagc
      961 acctacttta acatcaccgt cgttgacgac tgccaatacc tggtcgaata cgacattgga
     1021 atcaacatca gcatccaact tttcgacaaa gatgttgtcg ccttcctgga cacgatactg
     1081 ttttccgcct gttttaataa tagcgtacat gagtgacacc tcctcatcaa tatactcgct
     1141 ggatacggcg ttccctaaaa ggacgcttac aagcctctcc acgcggctta tccatagcca
     1201 cttatgctac gactcatcta ctatatcatg aattatcggg actgtcaata taaacgccct
     1261 tggaaactta caaaacagcc tttgtcccgg acagacaata tccccgtcac ggccgagccg
     1321 ataacgggga tattcgaaag aaacgggatt agtttccgtt gtgattaaag tagtccgcta
     1381 acccttttac aatgctttcg gcaaagttct gctgttgtgc ctctgtattg agggcctgtt
     1441 cttcactcgg attggagatg aagcccagtt cgaggagcgt cgccggcatg ttggtatgca
     1501 ggaggacgta gaagttggcc gtcttgacac cgcggtccga gaagttcgtc gccttcatgt
     1561 tctgctggtg aatgtagttg gccaggccgt aatcataagg cgtcttgctg ttgtaatagg
     1621 ccgtgacacc gccggcagac ggtgaatcga aggaatcgat gtgaatgctg atgaatacgt
     1681 cagccttggc ggcattgcca atattgctgc gggcctgcag ttcttctacg gcaccgtcat
     1741 acggagcgta aacgtcaacg tcagtcgtac gggtcatgac gacgttggca ccggctgcat
     1801 tcaacaagta agcaaccttc ttagaaatgg ccagg
//
LOCUS       DAWDXZ010000386         1834 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11077_length_1834_cov_7.381113, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000386 DAWDXZ010000000
VERSION     DAWDXZ010000386.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11077_length_1834_cov_7.381113"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1390..1399
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          438 a          435 c          412 g          539 t
ORIGIN      
        1 gcggccgcct cacgggtaat gagtccgttc ttttccatct gctttaagag ctccgttgcc
       61 gtcgacggcc ggatgccgaa ctcttcttca agatcttttt ggaagacgtc ttctctttgg
      121 gccagcaaaa agtgaagggt ccgcccttga gagccgctga attcaccgcg ggacggaagc
      181 atctctaact ttcggcgcag cttgttggac aatttgctga cataacttcc aatatgtcgg
      241 ttattcgata tcatggcacc ctcctatttc ttagtttcct aagtaattgt atctcaaata
      301 cgacggcctg tcaataaggc atccggcgca taaaaaatca ccgtcaagga caaaatccct
      361 gacggcgacg gttttcccgc tatagttcat gtatgcattt ttacaaaaag tggtggtcat
      421 gacacggata cgaggcttac caatccaatt ttcccgtcat gaggtagacc aagagtcctt
      481 tttcggcatg acgtctgttt tcggcttcgt cgaggatcgt ttccaaatga gcttccatga
      541 cgtcttggct gatttcatag cccggatgga tgggcatatc atggaatacc atggtgtccg
      601 tcccttccat gagggctgcg tcgatttgat agggcatcat catgttgatg atcttgtctt
      661 tcttttcggc gtaatcggga ttggtgaaaa attccatgtc gacccaggta tcggtgtaga
      721 cgcagtcgac gtcagcgacg actttcttga ggtcttcttt agaaatatcc gtcggcagtt
      781 ctttataatg gccggtctct gccaaggtgt cgtaaaagtc agccggcacg ttggtgatct
      841 gactgaaggg cgtcaagccg tacatggtac cgcccaattt gacgacgatt tcactgagcg
      901 agttgtaggt attattttta gccccgatat agaggacctt ggcttcaaag cgtcccgtct
      961 tttcatagag ggtcagcatg tcggccaggg cctgagacgg atggaaggta ttgtcacagc
     1021 cgttgatgac gggaaccgtg gcatatttca tgaagccttc cgtcgtttcg gccttcttga
     1081 accggcccat gatgcagtca acgttgcggc agacgtactt gacttcgctc atgaggtcgc
     1141 caatggtgaa gttgctgtcc ttccagagct ggttgtaggc cttgccgccc aattcttcca
     1201 tgccgatgga aaaggacaaa taagtccggt tcgacgtctt ttcaaacaag gtatataatt
     1261 ttttgccgtc gagaatatgg ctgtaagcgt cttgattctt tttaatcttg tcggccaata
     1321 ataagatgcc tttcaattct tcggctgtat aatcgataaa gctgttggcg tgtttcatgt
     1381 atatccatcn nnnnnnnnnt catagcacaa ggcctttcct ttatcaagta tttatacgta
     1441 attttcttta atttatacat ataaatcaat ttcatgcagc gcaagctcct ttccggagac
     1501 gcaaaaaacc ggtaatcacg atgattaccg gtccaaaagg tctatgtgtt actttccgat
     1561 taagctttaa ctacgttagc tgcctgcggt ccgcgcgcac cttcaacaat atcgaattca
     1621 acatcctgac cttcggccaa ggatttgaaa ccatcttcct gaatggcaga gaaatgaacg
     1681 aaaacgtcgc cgccgtcttc tctttcgatg aagccataac ctttttctgc gctaaaccat
     1741 tttactttac cgtgcatgat aaatcctcct aaaaatcaaa acaattacag ctatccatag
     1801 caagtgctac cttatagctc ttttgttagt gtaa
//
LOCUS       DAWDXZ010000387         1832 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11085_length_1832_cov_8.203714, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000387 DAWDXZ010000000
VERSION     DAWDXZ010000387.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11085_length_1832_cov_8.203714"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    394..403
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          532 a          464 c          497 g          329 t
ORIGIN      
        1 cgaagacacc gtggacgcca tagggcagga agggttccca attcatcggt tcgatatggg
       61 ggcctgccag gaagaggaac aagaaaatcg ttccgatttt aacgtagacc aaaatgcggt
      121 tgacgtgcgc actttcgcgg acgccgacga cgagcagcgc cgtcgccaat gcaacgatga
      181 gcacagccgg taagttaatg atgcctccat ctgccggaac ggccgtcaag gccttgggaa
      241 ggtcgatgcc catggaatgc aggatcccga cgaagtaggc cgaccagccg ccagcaacgg
      301 cgctggcacc gacagaatat tccaaaatca aactccaacc gacccaccat ccgaagattt
      361 caccgagcga tgcataggcg taggtatagg agcnnnnnnn nnntgcctga tacaggcaaa
      421 aacgaagcta attcagaata ggccaaacac acaaacgcac atgtgacggc tgcaaatacg
      481 aaggataaca tcaggccagg gccagcgtat tttgcagcgc cgataccggt cagaacaaag
      541 atccccgtac caataatgat gcccagaccc atcatggtga tgtcgaaggc tcctaaagcc
      601 ttgttcaact tcttttcttt cgtatgggcc agcaaatcat cgaaggattt acggcgtaaa
      661 atgtccataa gctcatcccc tgtctgttat ttcgctgtca caccgttaga cgaagaggag
      721 aaaatgcttt tccagataaa gccgatgacg aacccggcga tggtaaagca tacccagccc
      781 atccccaacg agtggagcgg taccatagta gcaaaaatag tatcaatctg tccaagcgag
      841 aattcagcag tctgaagtcc cgtaacgata gccggaatca aggtcaggcc gatagtccat
      901 gcatagacgc actgccgtcc gccaaaggcg ttgtgcagga aagccaagat gatgatggca
      961 acgacgagcg gatagatgaa catcaggacc ggaatagcgg cggtgatgat tgtcttcaaa
     1021 ccgaacatgg caacgaagaa ggaaatgagg gtaaagatga caacccattt cttgtaaccg
     1081 attttgatca gttcgttgaa gtacgatgcg cacgacgtaa tcaggccgat agacgtcgag
     1141 agacaagcca agagaacgat gacggccaag atgacagcac cggcgttgcc gaagagaatc
     1201 atagcactct tagaaagaac gggagcgccc gtttcctgga taccgaaagc ggcgacgctg
     1261 tcggcaccga ttttagcgac gaagatgtac acgaagccca agcagccgac ggcgatagca
     1321 ccggctttaa atacttctct cgtaatggcc gacggcgtcg ttgccccgtc ttccatgacg
     1381 aattcaataa ccaggatggc aaatactaag gaagcgatgg cgtccatcgt attgtaaccg
     1441 tcgaggatcc cctgaacggc ggcgacggta gccgtgccgt aagcggcaat cggttcctgc
     1501 ggcgagccga gaggcgtaat gaaggatttt gcgatgagca gtaaaatagc gatcagcagt
     1561 gccggcgtca agatcttgcc gatgcggtcg accagtttct gcggcgaaat ggataaccat
     1621 aacgaaatga ggaagaataa aaccaagaag ccggtcatga tggcttcact gccgccactc
     1681 tgcaagaacg gacgaacggc gatttcataa gaaacgctgc ccgtacgggg aatggcgaag
     1741 aacggcccaa tggtcagata actgatgacc gtaaagaaga ggccgtaagc cggatggacg
     1801 cggccagcca gttcctgcag gttcttacag cc
//
LOCUS       DAWDXZ010000388         1823 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11142_length_1823_cov_5.322964, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000388 DAWDXZ010000000
VERSION     DAWDXZ010000388.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11142_length_1823_cov_5.322964"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    250..353
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          448 a          472 c          403 g          396 t
ORIGIN      
        1 agtctctttt ttattctacc cttttacctt gcaaaagggc cgctaaaaat gcgataataa
       61 agagaacctt attgtacgac ttctgttttg acaaccgtgt ttttagaagc accatattga
      121 ggatgaatgc ctatgcagca cgataagaat aaactggcca tcagtgccgc caaattatac
      181 tatcagtcca atttcagcca agctgatatc gctaaggaaa tgaaattgtc ccgcccgtcg
      241 gtttcgaggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttgtacg
      361 aatcgaaatc ttcgacccca tcgaagacca gagccagttg gctcaaaagg tagctgcagc
      421 ctacggcctg cgcgatgtct gcatcgccaa cgctcccatt gaagatgaag aagaaataaa
      481 aaaatacatc ggccagcgcg gcgccgccta tgtcaatgac atcattcaag acggcgatat
      541 cattggcgtc ggctggggga caacgctgaa caacctgtcc catcaactca ttcctcaccc
      601 cttaaagggc gcccaaatcg tccagctcga agggggcatc accttatcga ccagcgaaac
      661 ctatgccaat gaaatcctgg ggaaattcgc cagcaattac agtaccattg cccaatatct
      721 ccccctcccg gtcttatttg attctaagga agtcaaggaa atggtctacc gcgaccgcca
      781 tatcaaacgc gtcctcgagc tcggccgcaa tgccaacatc gccatcttca gcgtcggtac
      841 cgtccgcgac accgcccttt tcttccgcct gggctatgcc gattcacaag aaaagaacta
      901 tctaaaaacc cacgctgtcg gtgacatttg ttcccgcttc ttcgacaagg acggaaacat
      961 ttccagccag gaattgaatg accgcaccgt cggaatcgac ctggatgacc tacgtcataa
     1021 agaatacagc atcttacttt ccggcggcga tggaaaaata gccagtatcc gagcagcctt
     1081 aaaaggcggc tatgccaacg tcctcattac ggaccagttc accgcaaaaa aattattatc
     1141 agaataaaag aacgccctgc cgtactacgg cagggcgttt tgtcataagt aagagctcat
     1201 cagcctattc ccaagcactg tcaggaaggg ctgtaatatc atcccaagca ttgttaatgg
     1261 ctcgggccat cagttctttt cccggcggat ccagatgaag tccgtctaag gccagctcgg
     1321 tcttgagttc gccgtcttca tcggccattc cgggtacgat atcgatgtga acctgagtat
     1381 cgatataatc gttgacctcg gcaatggacc gccgccaatc gtcggtcgta tcctggtcga
     1441 aggcggcctt gatattttcc gggttgagag gcggaatcgt caggaatacc ggttttattc
     1501 cgttctccag ggcttgtttt ttaatcgacc ggagatcgcc gatgacttcc cacgacggga
     1561 caccggcccg cagactgttc gagccgacga ggaccagtac gtacgacggc ttgaagggca
     1621 ggacatcttt ttcaaagcgc tcggcagcca tggcactcgt atcgccgctt tgagataaat
     1681 taatggtcgg aaaattcaag tacgaagcgt agctgaattc ccaatccgtc ggcgaatagg
     1741 aaacgctgcc gccgccgtga ctgatactgt cgccgaaaat ggcgacttcc ccggcttcgg
     1801 tcttgggact cagctcgaaa ggc
//
LOCUS       DAWDXZ010000389         1823 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11140_length_1823_cov_8.171380, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000389 DAWDXZ010000000
VERSION     DAWDXZ010000389.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11140_length_1823_cov_8.171380"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          485 a          417 c          473 g          448 t
ORIGIN      
        1 tggtggaaag cattctcgaa gaccgtgcac gggagaaaca ggcagtggct gtcttagaaa
       61 atatgctttg ctccggcgac tgcgccgccg ataaacggga agcctgccct tattatcagg
      121 atcatctgtc ccgctctgac gacgaggtga cggtattgaa cgattccttc cagctagggg
      181 ttaactatga agaagcccgg tttcagcgcg atgacatcga cgcccattat accgcgttga
      241 aggaggaata tggaacgact tactggtctt atgccgctca ccatattatt tccggaggcc
      301 aggtttttaa acaggaaccg gaaatcgtac gcttggcaga ctttctccac tatgatatca
      361 acaatgctga aaactgcatc ttcctgatat caaagggcca gggctatgga gatgaaacga
      421 aggccgaacg gccgatcagt gcctatgatg ccatgggaag ggcccgactg cagtggcatc
      481 tcggcggcca tcaatatacc ctgccggcag aagaactgga actcattcgc cagcggattc
      541 aaaagcagac gggtcagtca gcggcggaca ttcagtctta tgcggacttg cttcgacaag
      601 acatggcgga ctttaaaatc tggttgaggc agcatccttg ctggtacagt caggatgaaa
      661 cgaagcagaa ggctttggcc gccaagttta ataagggtat gcatcggatt tcacggcgaa
      721 ttcgccgaaa actcggggat tttacggaaa agccgagcag cagttatcct tattacgtat
      781 cgaaagaagc ctatcgctat acctttaccc tacccaagac ggaaaaatgc atcattgtcc
      841 gtcgaacccc taagggcatc cgattgtatc gctatcggat cctgcgggat ctcaaggctg
      901 atgacggatt cctgatttta aagcccatcg aaaagaatca gtcgccctat gtatttgacc
      961 taccgctgcc ggtctcggac cgtccggcat atcgggaaaa gcagcggcag tgcattgaat
     1021 tttgtgacaa tatcggtcac ttcttatgta tcgatatgga tcaggcagaa gggctgccct
     1081 tccagccgga ttttatcaaa aggatatatt gtgaaccttc ggagcaggat cacagcaatg
     1141 aagacctgtt gaggttggaa aagcgacagg cagaattgtt ggcttggttc cgggactgcc
     1201 aattcgagca ctatatgtct ccggtcacga tggttcgccg gcgattgaaa gaatggaggg
     1261 aacgatatgg ctgatggata ttctttttat cgcctctatg gagatacggc agccgaggaa
     1321 tatgcccggc ctgtccataa attgtatcag cagcttgaac gaggctgtgc ccgctcggag
     1381 tttcaatttt tgaatcacga gaagctcgaa gttcctttgg acgagggcgg cctggacttt
     1441 ccggattttt tatactatga acagattccg ctggtttcag acgctttata ccgatttttt
     1501 tctgacagcg gtgtcgataa tttattctac aaaccgattt acttgaatga cgagatcatc
     1561 ggagtccgca aacgatactg gctggctctg ccaccgcgca tccgatgcct taattatgaa
     1621 gcaagtcagt tccgcgattc agatgcaccc ttgaagcggg ctgaaaaaat cgtcatcaat
     1681 cccgccaagg tgggaaatta ccgaatattt tgcatagagg aggtcattaa ccgcgatatc
     1741 attataacgc aggaattgaa ggaaaagctg gaaaaagcgc agtttacgaa tttgcttttt
     1801 gagacaatgg aggattgata gta
//
LOCUS       DAWDXZ010000390         1823 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11141_length_1823_cov_7.728507, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000390 DAWDXZ010000000
VERSION     DAWDXZ010000390.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11141_length_1823_cov_7.728507"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          532 a          394 c          458 g          439 t
ORIGIN      
        1 ttcatattat ggcgctaagg ttcaattgga agcagatcat gatattacct ttaaccatac
       61 ttatagtaat actccggcgg tacagggcct gccgattttg tattttggga ataatgctga
      121 aggaacgata acggccggta acgatattaa ttttaaccgc aatcaatatg cgtatttaat
      181 tggtgtgcag tatagtggta aggctgttgt aacagctaag ggtaatatta atttccgtga
      241 acaggcaccg ggttatatgg ctgttatgtc tgcagaacgt ggggcgaatc ttgaattgca
      301 ggcacaggcc attacgggga atacgcagca aatcgtgtca accacgtatg gttcgcaggc
      361 actgctcaag gcgaatgaaa ttaattgggt agccgaagga agagatatgt ctgattatgc
      421 agagcaatac ccgtataaga ccagtatgct cgtagcctat cgaggcgata gtaaggttga
      481 attacaggcc gataaggcca ttaatttatc aatcggtgta ccccatacca tgctctatgc
      541 tgagggcggc gatattgctc ttaatgccgg cgattcgatt tatttgcatt ccaccggagc
      601 gacagacgaa gcttctcagg ccagcctgcg tgtagttaat ggggccattg atttgacggc
      661 cagtgcgatt actttagaaa atgaaggggc aacggcagct tatgcagagt atttggggaa
      721 tattaatttt aatggcaaca cctttataaa gggtgccggt atcggtgctt tttcgcaagt
      781 aggcggcaaa attaacttta acggcaatac agttatgtcg caagtcggta ttggggcttt
      841 ggcagaaacc gacacagatc catggaatga agatctttca aagattactt ttgatggcga
      901 tgtcgcaatg gctcaagctg aaacaggagc cagtgtcaaa tataatagcg acatcgtttt
      961 caacaaaggc gttgctattg atgccaaaga caatgccttc tatacggaat cagccggacg
     1021 tatccaagcc ttgacttccg gtgtcgataa ggttatcaag ggcaatatgc aggccctgaa
     1081 tggtcagatt gacgtcctct ttgacacggc aaattcatcc tttaccggca agacggaact
     1141 tcaaaatatt gaaaaacttt acgtcattga tgacgaagaa gagtacgacg atgaggattt
     1201 agacgtcgac acctatgcag ccgatgatga agaatacgat gacagtgacg atgactggga
     1261 agaaccagaa aacccgtata tcgaagaaac gccgcacatc aatatagccc tgaaaaacgg
     1321 cgctgtttgg aacgtaactg gtgacagtac gctgaccaat ctggccaatg attcttatgt
     1381 caatatgacg gacggaacga aaagcggcac cagccttacg gcccagacct tatccggtac
     1441 ggggactatg gtcatggacc tcgattggac cagtaacggc ggggcaaaag aaaagaccga
     1501 taacagcgat tacctaaatg ttacagaaag tgcgaccggg acacaggccc tggtaactga
     1561 caaagcatcc atgcatctcg atgccatggg cgtcaatgac cgcctctact tcgctacttt
     1621 ggccaacagt gatgccgtat ttaccagccc cattacccag cgcaacgtac aaaaaggcca
     1681 tttatacgac tacattatcg gcatcgattc tgaaacgacg gctacaacgg atgcaaccga
     1741 tgcggttgaa actgtcgctg acagagccgc agcggatacg acgacggatt ggttcttcgg
     1801 taccgtcggc tataccgaaa gcc
//
LOCUS       DAWDXZ010000391         1811 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11214_length_1811_cov_9.268223, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000391 DAWDXZ010000000
VERSION     DAWDXZ010000391.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11214_length_1811_cov_9.268223"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    477..486
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          289 a          532 c          554 g          426 t
ORIGIN      
        1 cgacgtctga ttctatggca ccggaactgg ccgaggcctt ggcagccgta ccgtcggtgg
       61 gcatcattca tcggcctaat ttagagacga ttttatcgct ccatccggat ttagtcgtcg
      121 gcatcaacgt gccttttcac aaccagattc gccaaaccct ggcccaaaac ggcatcccgc
      181 tgtacatcaa ttccttggat tccttggaag acagtctgag gacgctggag ttctttggcg
      241 aattgaccgg ccgtgaaccg gaggcagaga agaaggctgc cgatatcaaa gatcggaccg
      301 atgccgtcat ggcctctgcc ggcgggaagg aaagcccgcg ggccctgatc ctcttcagtt
      361 cgccggacag caacaatatg gcgacggcag ccagcttttc cggcgatatg ctgaaacgcc
      421 tccacggtac caatattgcc gagggcgccg gttctcacga gccctttgtg cccctcnnnn
      481 nnnnnntcag catggaatac gtcctgcgcc aagatccgca ggtcatcttt atcatctcca
      541 tgggccagag tcccgatgag gccgatcgat tccggtcatc catggctgac agcgattctt
      601 ggagtcaggt ccgggctgtg cgccaaggga aggtctatgc cctgccgacg gccctattta
      661 cggtcaatcc gggaagccgc attgccgatg ccatggaata catggccgac cgcctgtatg
      721 ggggagggac cctatgatgg cggataaggt cacccggaaa agagagatcc ggcggctgac
      781 ggtcctctta gccggcctgg tcctcttggt cggtgtcctt attttaacgg tcgtcgtcgg
      841 tgccgtatcg atccccttac cggatctttg gctggcgttg cgggcgccgg ggacgacgga
      901 agattaccgt atcatctata ccctgcgcat gcctcgcggc atctgcgccg ccttggccgg
      961 ggccaacctg gccttgtcgg gctgcatcct ccaaggggtc cttcgcaatc ccttggccga
     1021 tccgggcatt atcggcattt cggccggagc gggcctggcg gctatggcct tgatgatgct
     1081 ggcgccgacg ctgaccgttt gggtaccttt agcggccttt gccggagcct taggggcggc
     1141 ggccatcgtc tttttcctgg cctgggagcg gggcgtcaat cccctgcgcc tggtactggc
     1201 cggggtggcc gtggcggcct tctttggcgg cggcatgacg gccttgtccg ttttcttttc
     1261 cgataaggtc caggggaccg tgtcctggat ggccggcgga tttgccggtg ccggctggca
     1321 gagcgtgcgc atgatcctgc cttatagtct gatcggcatg gtggggacaa tctgcctcta
     1381 tcggcgcctc aatgccctgc agctcggcga cgacgtagct cagacgttag gcgttcaggt
     1441 ccatcggacg cgggctcttt tggtagccct ggccgcgctt ttggcggcgt ctgccgtcag
     1501 cgtagccggc cttttgggct tcgtcggact ggtcgtgccc cacgtgatgc gcctcatcgt
     1561 cggttctgat ttcgagcccc ttttgccttg ctcggctgtg ttcggtgccg tcttggtagc
     1621 cggcgcagac gctgcggcca ggacggtctt cagtcccatg gaagttcctg tcggcatctt
     1681 catgtccttc atcggggcgc cgtttttcct gtatttattg agacggaggc tgggtacgcc
     1741 atgagtccta ttgaacttaa gggtcttcag atccaatacg gcaataaggt cgttgcccgg
     1801 gacttgaacc t
//
LOCUS       DAWDXZ010000392         1797 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11298_length_1797_cov_6.672216, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000392 DAWDXZ010000000
VERSION     DAWDXZ010000392.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11298_length_1797_cov_6.672216"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          371 a          503 c          486 g          437 t
ORIGIN      
        1 atgtttaaag gctgtcttcc aagcttcaaa gaagttcgtg acgccgcgag aaatgaggcg
       61 gcggatgacg tcttttaagc gatcgatgtc gacggtgagt ttgactttga ccctattttg
      121 attttcttct cgttccctct gttggccctc atcgtcactg ttttgcttgt ctcttcgtcc
      181 tgccgttttg ttaccgtccg taaacagcag cagtgtccac tccttgtctt gccaagcctt
      241 gatttcttcc gttcgctctt cccaaatggg ccgcatggct tcggccaggc atttgcggtt
      301 catgaaagaa atctgcgtcg cttcccgctc ggccaagatg agggtctccg tccccgccgg
      361 ggccgtgcag ccatgaggca gggcgatggg gatagcattg tcgcgcataa agtccaagtt
      421 ggcacagaaa acttcgaagg ccaggacatt gagcaggttt tcgcctccta aataggggtc
      481 gccgccgcgg ccaaactgat ggagctcgaa cttatggcgc cgcggattgg ccgaccgttt
      541 ttcgatgccg aagtcaaagt ccgtcgtgcc gccgccaaag tcaaagacgc cgtaagccgt
      601 catctctccc tgttcagccg gttccagtcc ataggcttgc agggccgctg cagcataggc
      661 tgccggttcg ctggccccgg catagacgcg gaagcgccgc atgcaatttt cgtcggccaa
      721 gatagccggc ggcagggatt ttttcagacc cttctcgaag cttttcagca actgtgtacg
      781 aacagccagg ttgaccggga aggacaggat gtagtccaaa taaatgccat ggcgcatatt
      841 attgatatag aggcctaagt aataggcgta gatttcgatg gggtcaaggg cttcatcgtc
      901 taattccaag tagggattga gcttccatac cttgcccttg cggtctttga gcagcagtcg
      961 ccggtctttt tcatgggccc actgtttcaa ttcgctgaag acggcgtaat agacggaact
     1021 gtccacatcc tgctgaagca gggcctgttc ggcctgatga gatacggtca cgtcttccca
     1081 agccgtaaag ggccggccgt tccggctgcg ataggccttt tcaaaactgt ctaagtcgcg
     1141 gagttcgacg acggtcggat tttcataatc cttctgctcg acggccttgg taaagtcacc
     1201 ggtgccgatg cgcaggagcc gtgttccccc ttctcctcgg cagacgacga cggaactctt
     1261 ggtgccgaag tcgatggcac aggtcccgtt ggccacgaca tcgagttgag gcggccgagc
     1321 cgtccagacc tgattcggcg gaagaggaac ggcaaccgca ccgtcttccg gagcttcaaa
     1381 gagttcccag tggcccttat tgatatcgga cagggtactg tcggcatagg gttccaaatt
     1441 ggcccgccgg tagtcgcatt ccagaagctg cttccgcagc aaaggcaaat ctatatcact
     1501 ctgacctttt cctggcttaa gcccctcttc cgatacttcg cccttccatt gaaaaacatt
     1561 cttccgaaaa cggccgtcac ggacgtcatc catgaaggcc tctcttcgga aaagaatgcg
     1621 gtcgttcgcc gaaatcccat caagataggc gtcgaggtct tgataggatg acaaaagctg
     1681 atgataggcg gtctcttctt tctcggtaag gccttccggt acgaggcccc tttgcagcca
     1741 tgccgacaac gtcttgatcg gattcaagcc atcgctgtcc ctgccgtcca aacggga
//
LOCUS       DAWDXZ010000393         1793 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11316_length_1793_cov_5.864212, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000393 DAWDXZ010000000
VERSION     DAWDXZ010000393.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11316_length_1793_cov_5.864212"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    980..989
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          394 a          413 c          468 g          508 t
ORIGIN      
        1 gtcgctggcc tgtttttcga cagggatgct ttcaccggta atggccgatt cgtcgacgct
       61 cgtcgagcct tccgaaatga tcccgtcagc ggggatgctt tcgcccgggc ggatgacgat
      121 ttcatcgccg acgactaatt cttcgacggg aatgaccgtt tcgattccgt tacgaaggac
      181 cgtcgcctgt ttcggtgcca gatccatcag tttttcgatg gccgtgctgg tcttgccttt
      241 ggcgcgggct tccaggtatt tgccgatgtc gatgagggtg acgatcatac cggccgattc
      301 aaagtacagg ttcatgctgt attgagagac cagtgctaaa tcgccgtggc cgaggcccca
      361 gctcatgcgg aaaatggcga agacgccgta gagggcagca gccatcgagc ctaaacctac
      421 cagactgtcc atgttcggcg ccccttcaaa gaggttgcgg aaaccgttga tatagtactt
      481 gcggttcagg tacaggatgg gaaggattaa taagaactgg gtaaatgcaa aggtaatggc
      541 gttttgcggc ccatgaaata tgtagtgcat aaaattcgga ataggaagac cgaaccattc
      601 attgaacata tgatacatcg aaatgtacat gaccgggatc aagaagatga tggatccgat
      661 gagacgctgc ttcatggact tcatttcttc ttttgctaaa tcggaggcgc tcttctgcgg
      721 cgctccggat gaggcggaac ttcccgtatc ggccgggctg gcaccgtagc cggcatcgac
      781 gacggctttg atgatatctt gctggctgac gacgttttcg tcgtagtcgg cctgcatgct
      841 gttggtcaag aggttgacgc tggctttatc gatgccgtcc agcttattga ctgctttttc
      901 aacacgcgtt gagcaggcag agcagctcat gccggtaatg gtaaattttt cgtgtttcac
      961 taggctcact cctttatttn nnnnnnnnnt ttcatgagtt tttgcaaggc tccgacgagt
     1021 tcatcgacga cttcgtcttt gccatcgcga atatcatcga cgacgcatcc cttgatatgc
     1081 tgacctaaga gctctttatt aaaggcattc aatgccgatt ggatggcact gacttgagtc
     1141 aggatatcga tacagtagcg gtcgtcttcc aacatgtttc tcacgcctcg gacctggcct
     1201 tcgatacggt tcagccgcga tacgagagat ttatactctt tgccctcttt gctgcggtac
     1261 ttatgttttt cagaattgca gcagggacag acatcttttt cttccatagt attggttccc
     1321 tccttagata cccccttggg gtacattgtt atgatatacc caatgggggt atatgtcaag
     1381 agtgagtgtg ggattttgat atatttttac aaaaaggttg gaaaagggct ttgttatagc
     1441 ttctataggc acgaagaagg cacaaaaaaa gactgccacg aaggtgacag tctttggaaa
     1501 atcgatgatt ttattttaac aaggctttga cggcatccgt ttcgtcggta gcattttcag
     1561 caccggcgat gatagctccc tgagcgacga tgctgtcgag gcctttttct ttgcggacct
     1621 gttcaacttt agagacgacg tctttttcaa tcggaacgac gagtttcgtt tcaagctggc
     1681 tgaggttctg tgccaattca cggccgaggt tctgtctttc ctgatcggtc ttcagtttcg
     1741 gggcctgttt agagaacttg tcgttagcgt ctttaatagc cgactgcatc tga
//
LOCUS       DAWDXZ010000394         1780 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11390_length_1780_cov_9.451594, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000394 DAWDXZ010000000
VERSION     DAWDXZ010000394.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11390_length_1780_cov_9.451594"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    591..600
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          394 a          446 c          475 g          455 t
ORIGIN      
        1 catgaagcat cataagtaca tccccttttc tgctgaaaat gattattttg atttctgctg
       61 gtatgccgca atatcggcaa attcaccttg taatttttgg tagagacgag tgaaaatatc
      121 gtagagctca cggtatacgg cggcgttttc ttcaatgggc gtatagactt tcttggcgtg
      181 aaccaagttg gccgtatcgg cgatgctctt taatttaccg cttgaaatga agcccaggac
      241 ggcggcacca aaggctgcgc cttcactgtt gtcgggcaag gtaatcggtt cattgaagac
      301 atctgacatg atctgaagcc agagcttcga tttggtaaag ctgccgctga cccggatgtc
      361 cttgacctcg ccgaattccc gcagggcggc catgacgctg ttcaagctgt agcaaatgcc
      421 ttccatgacg gcacggatca tatgggatcg actgtgattg agggaaaagc cgaagaacat
      481 gccccgcagt tcggagttcc agtgcggtgc ccgttcgccc gtaaagcaag gcaggcagat
      541 gaggccgtcg gcaccggcat cgacgtgttc ggccttcatg gtcatcaggt nnnnnnnnnn
      601 tcttgtcacg gacccagcgc aggatcatgc cgccgttgtt gatggccccg ccggcgaccc
      661 acatgtcgtc agtcaggttg tagcaccaag tacgcatctg cggatcgatc ttaggttgac
      721 gggtcagcat gcgaagagcc ccactggtac cgatggtacc gctgatctgt cccggttcga
      781 cggcaccgat gccgacgttg accaagacgc cgtctgtggc gccgatgacg acgggaaggc
      841 cttcggaaag gcgaagatcc ttggctgcct tttcactcag tccatggctg tacgtcgtcg
      901 agacgacggg cggaagctgg tctttggtga taccggcata ggcgatgatt tcgtcgtccc
      961 attcaagggt gtgttcgttg tacatgcccg tcgtgctggc cgtcgactta tcgacgagcc
     1021 actcaccggt cagatgatag tataagtaat ctttcaggga accgacgaaa cgcatttggc
     1081 ggaacagttc gggctggttc tgacggagcc agatgatttt ggccaaggga tagcaggcgt
     1141 gaatcgggca gcctgtccgt tcgtagaaag agcggcacaa ggcttcgtct ttcttcatgt
     1201 cccggacgat ggcagcactg cggctgtcgg cccaggtctg catatcatgg agaggttcaa
     1261 aattttcgtc gagggcggca aagctgtgca tggccgtact caaggcaatg cctgccagtt
     1321 ccttattctt atagcggagc tggcctgtcg tcttacggat gacatcctgg acggcttcga
     1381 gaatctggag cgggctttct tcggcccaat cgggacgagg tgtcaacaac ggataaaagg
     1441 cttcggatga gcagacattg atcccctctt ccgtataagc aatggcacgg acaccggtcg
     1501 ttccgacatc gacgccaatc cagacttcct gattggcgga atccatcggc gtaattgtat
     1561 tcataagtca tacttccttt catgaatcat ggctagtctt ttcgatgccc ttatgatacc
     1621 acgcctattt cctaaatgca ataaaaatat catttttaat tttatatttt gatattttta
     1681 ttgcataaga aagacttttt ctatgctaaa aaggcaatca tccttcctga cgatgaccgc
     1741 cttgctccat ttcccttcct gtggtatagt tatcttggta
//
LOCUS       DAWDXZ010000395         1778 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11407_length_1778_cov_6.318630, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000395 DAWDXZ010000000
VERSION     DAWDXZ010000395.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11407_length_1778_cov_6.318630"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    684..745
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          439 a          390 c          432 g          455 t
ORIGIN      
        1 atttttaaaa acataaaaaa attggacccg gaatagaatc cgagcccaat cttagggaag
       61 agaaagagta tgattatttt acgtcagtgt tgttaatgct ttcgaccaaa gcgtcagcta
      121 agtgttccag ttctaccgct ttgtcgtcat gcatggccga agcgagggac aggcgttcat
      181 tgaggatggt catgttggaa aggttattgt caatgaattc ctgcatcaaa tcgccggatt
      241 ttacagccca agagccgttt tcgatcaagg caatcgtccg attctgtacg tgaagagcct
      301 tcatgtgttc gaggaagtcc agcatgaccg gatagatgcc caggttatac gttaccgaag
      361 ccagtaccaa gtgagagagc ttaaaggact gggaaatcaa ttccgaaacg tgagttgaag
      421 aaacgtcgca gatccggata ttggtaacgc ccttttcgca aagtctcgaa gccaagttct
      481 gagcagcagc ttccgtgttg ccgtacatcg aagagtaggc aatgaggacg cctttttctt
      541 ccggtgtata cgtgctccag cgctgatatt tgtcgagcag atagccgaag tcttttctcc
      601 agaccggacc atggagcggg caaatgactt tgattttatc caagaccgtc gcagctttgc
      661 ccaagaggtg ctgtacgtga gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnatttg ccgacgatgt tggtgtaata acggcgggct
      781 tcatcgagcc aatcgcggtc gaagttgact tcatcggcga acagtttgcc gtcaagagcg
      841 atgaaggaac cgaaggcgtc tgccgagaag aggatgccgt cggtgaggtc gagggtgacc
      901 atagcttccg gccagtgaac catcggagct tctacatagg ttacggtatg tttaccgaag
      961 gtcgtcgtgt cgccttcctt gacttcgatc aattcctggt tatcgatttt aaagccgaac
     1021 tgacgcatga gcatgaagga tttttccgtg ctgatgattt taacattcgg ataacggagg
     1081 acgatttctt ccatgctggc gcagtggtcc ggttccatat ggttaatgac catgtaatcg
     1141 aggtcgcggc cgttgagaac gtgttctacg ttttctaaga actgacggca gacagcccag
     1201 tcaaccgtgt caaacaatac cgtcttttcg tcgagcaaga ggtaagcatt ataagaaacg
     1261 cccaagggaa tggggtgaat attttcaaac aaatgtaatc tatggtcatt agcaccgacc
     1321 caagaaacat cttttgttac tttgcgtacg caatacataa tttatcgact cctttcgccg
     1381 cactattctt gaataaaatg actcttttct tcagcgcatt ccgggcaaac ccaatcatca
     1441 ggcaggtcct tgaacaaggt tcccggtgca atatcagctt cttcatcacc taattcagga
     1501 atatagacat agccgcagcc gttgcagaca tatttcgggc caatcggttc cggtttagga
     1561 acgaccgggc gtttcatctt aaccatcggc ggagccggct ttttatcttc caggccgagt
     1621 tcctgtgcta agagcggcag gatttcctga acgtcgccga cgataccgta gtcgcagttt
     1681 ttaaagatcg gagcgttgcc gtttttattg atagctacaa ttgtagatgc atctttgatg
     1741 cctttcaaat gctgtttcgc accggaaata ccgcaggc
//
LOCUS       DAWDXZ010000396         1777 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11412_length_1777_cov_9.222997, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000396 DAWDXZ010000000
VERSION     DAWDXZ010000396.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11412_length_1777_cov_9.222997"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          383 a          477 c          505 g          412 t
ORIGIN      
        1 gacgatgagc cctcccactt accttaactg catttactcc agccgcagtt cttgcaggta
       61 ttgcagccac cttcgaaggt caagggttcg ccgcactgcg ggcaaaggat gctttcagcc
      121 gtgactttct tttcagctct cgggcggacc ggagcttttt tcaccggtgg ggtatgaccg
      181 ctggcgacgg attccgtctg ctgcagttcc tgctggactt catcgaacat gtcgaggagg
      241 gcattgccga cggccatggg gcagcacgag ccgcggctgg tatcaccgcg cgtcacccgg
      301 cggaccgaat aggacgggca gttccccgtc gaattgagct gatcgacgat agtatagata
      361 tcgatgccgg cacgggcgct gatagaaatc atacgcgaca ggccgatcat gaagttattg
      421 cagccgcccg tagatccctt actcaaatac gtttcaagca gagccccggt ctggggatca
      481 aagaaggcca tgcagtgcag gcggccgcag cccgtaatga gcttgcgctt cttgccgatg
      541 acgttgtcgt cgatgctgac gatttcaccg cgttcgaggc cttcgccggc cgtcggtacc
      601 ggtttttctt cttcatgcgt cgtcaggatg ccggcccgtt tgcagccgtc acggaataag
      661 gtcaggccct tgcagccgtt ttcataggcc aagaggtaga ggttttccgt atcttcgacg
      721 gtgaagctgt tgggtacatt gactgtcgaa ctgatggacg catcgatgtg acgctgccaa
      781 gcagcctgca tggcgacgcg ttcgcggtag tcgagggtca aggccgtgac gaagtaatcc
      841 ggcaattcac cgtcatcttt cagcttacgg gccttcatat accgttccac gataggcgta
      901 tagactttgt agtacacgtc cgtcccgtga agggattcag tttttctttc gtagaaattg
      961 gcgtaaatcg gttcaatccc gccggaaatg ccgagcatcg tcgacaaggt tcccgtcggc
     1021 gcgatggtga gcaactggga attgcgcagg ccgtattcct ttaccttttc agccgttgca
     1081 tccgtcgtat tggctttaaa atacggtgtg ctcatgattt catcgatatg gcatttagca
     1141 aaggcacccc gttcgtgtgc cagatccgcc gaagcggcaa tggccgtatc cgccatggca
     1201 aagccgatat catggcagaa atcaacggca tccttactgc cgtaggtcag tcccatctga
     1261 atgagcatgt caccaaggcc catgataccg aggccgatct ggcgccaagc cgtaacgctt
     1321 tcccgctgtt cttccaaggg atggagggga aggccttctt caagaacgtc atttaaagcg
     1381 cgaacggcga tgacgacgca gcgcttgaat tcgtcaaagt caaaggaagc attcggcgta
     1441 aagggggatg ttacgaagtt tgccaagttg aggctgccga ggaggcatga accaccggcc
     1501 ggcaagggtt cttccgcaca gggattgaca ccggcaaagg agaattccgg cgtattggcc
     1561 aagaggttcc acgacttgat gcggtcccaa aagagggctc ccggttcggc ataatcccag
     1621 ttcgtttcag caacgagacg gaatacatca gctgcgttga ccaagctttc gatgacctgc
     1681 cccgtttctt tacgggtgaa gtgcaggcgg aactgcttat gctccttgac ggcttccatg
     1741 aattcatcgc taaggcgaat agaaatattg gccttcg
//
LOCUS       DAWDXZ010000397         1773 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11439_length_1773_cov_7.663562, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000397 DAWDXZ010000000
VERSION     DAWDXZ010000397.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11439_length_1773_cov_7.663562"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    448..457
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1265..1274
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          405 a          446 c          437 g          465 t
ORIGIN      
        1 ccagaagtag gtatcgggaa gttctaagtg ttccagaaaa gtcttttctt cttcgagatt
       61 ttctctctct tcggccggga tgaagacgcc cttttttatg tcgtcgtcga gtttcgttcc
      121 ggcaaaggcc gacatggtgt tgaccaaaat ttgagtcggc ttgatttcgt tctcaagctt
      181 ggccatggcc agggcgtttt cttgaccccg accgcggccg ccggtgccca gcatcaataa
      241 atcggagtga cggataccga cggcattgag acgcaggcac tgctcccgga tttcttcgat
      301 agtatagcct ttattgaggt attcgaggat atccgtcaaa ccgctttcga tgccgatgta
      361 gaggtcattg acaccggcgg ccttcaaggc cttcaaatcg tcatcgctct tatgggcaat
      421 attttcgatg cgggcataca tggtaatnnn nnnnnnngga aggtacttct tgatgacgtc
      481 gatgcgctcg atcaaattct ttgccgacag gacgaaagga tcggcgccga taagatagac
      541 ccgttccaag tcttccatct gccaaccata gcgttctttg gcacctttca tataggcttc
      601 gacgtcagcc gtcggccaca tgcgaaaggg aacgtccttg tacatgctgc aaaaatgaca
      661 gctgttatgg ctgcaccctt ccgtcagggg aatcaaaaac gtattggcct caataggcgg
      721 ccgatatata gttccgttat attccatgtt cttctcctcc tatcaatatt caccgggagc
      781 gcggtagcct tgggatgcgt actccgtccg gaaacggcgc agggctgctt ctccttgatt
      841 atctataata tcttgaatcc cgtcgatgag ctcctgttta tgggccggaa tctccgtccg
      901 tacgtggaaa ggcacggaaa tgtgactgga ctgaaagatt gtgtcgttgg tcagggcttt
      961 caagaattcc tgcatttcct gcagccgttc gacttcgccg gcttcttcat attttccggc
     1021 ccgggccaag ttccataagc ccgtcttggg gatgaggacc agcgaagaaa tatcaatcat
     1081 cgacgggtgg aggccgtcat agagggacgc cgtatcgagg gcatgtttga gaccataccc
     1141 cttcccgccg aggccgttga tccagttgac gacataagag aagccggcgc ggtctaattt
     1201 ttccaattgc tctcttgccg tcgcccgatc atagcccttg tgcatgaatt tcaacaaatg
     1261 atcgnnnnnn nnnnatcgat gcgggcgtag ccgccaatcg tttcaacgga cggcacatag
     1321 cggtgaatca gctccgccgt cttcatcaag acgtcgtaat cggcagaaaa agcgtcggcc
     1381 gactgcagga aaattcgctt cggtgcgcca aagtatttgg ggatttcctt gacatcggct
     1441 tcgatttctt ccatgggcga ccgcttgaat cggtcggtct tatagtaact gcaaaaataa
     1501 cagccgttat gagaacagcc gctcgttaat tgtaaaaatc cggaattgcc ttcataaggc
     1561 ggccggttta ttccactggt aaaatgcatg gtggcttcct cctgtatgct ggatcgtatc
     1621 atcaaaaaac aatgaggcct aatatccggc aggcatcgcc ctgcccttac ttcttaccga
     1681 caagattgtc ttgccctttc tcatcgtatg atgtattata accatgaagt acgacaatga
     1741 caagtacgca catttttggt acctgaaagg aca
//
LOCUS       DAWDXZ010000398         1757 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11545_length_1757_cov_11.704465, whole genome shotgun
            sequence.
ACCESSION   DAWDXZ010000398 DAWDXZ010000000
VERSION     DAWDXZ010000398.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11545_length_1757_cov_11.704465"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1459..1468
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          312 a          496 c          478 g          461 t
ORIGIN      
        1 tcgataagtt ccgatgccaa gacggcagcc tgggcttgta agaaggaggt ccggacgctt
       61 tcatagtcga cgcggtcgtt ccgtgccttt tgagcggctt tttctaacga ctgctgaagc
      121 tgctgccact gtttctgact gtctgacttc tgaaaggcca gggcagctgc ttctttttct
      181 cgcgctaccc tttcttccaa ggtccggcac tgctgcttca cctgttcagc aagaacggcc
      241 ttttcagaca aggccgacaa ggcgacgcgg gctttctcta aatccttttt ccccgattct
      301 gtttcgccct tagccttctt ccaggcctgt tcggcagcca ttgcttctga ccgaagcttt
      361 tggacctgtt ggcactggtc gtagtaagaa tcggctttgc ccttgagatt ggtcagcttg
      421 attcgttctt ctccggccgc cttataggct ccttcctgct tagaaagttt ttccatcttc
      481 tcgttagcgg ccttcaagtc gacagccgcc tgctgtgccg cttcattggc tttgtcagca
      541 gccttggcgg cagccttccc ttcccgttcc agctcatcga ggtggcggca gggttcggct
      601 aagacctcgg cttttctcaa gacgtcaatc ttattccgct tatcctgaaa aacccctgct
      661 tggccatcga gattggctgc ttcctgccgt ttctgctccc acagtttcca gtgggcttcc
      721 aaggcctggg ctgcctgaaa gtctttctgc cgagcactgc gctcagcttc ggctttgtgc
      781 cacttttcgg acacggaaac ttcctgatcc cgaaattcct gctctcgttc cgaaagtttc
      841 ccttcatcgt cgacgccgag attgagcaaa cattgttccc gatcgcgggc cacctgttca
      901 tggcgttttt ccagttcgtc atggcgttct ttagccaggg cctggagtct ggcataggac
      961 tgggtatgaa acagggtctg catgatctgc tgacgctcgc cggagtttgc caataagagt
     1021 tttcgaaagt cgccctgcgg caggaggacg acctggcgga actgatcggc tttaaagccc
     1081 aagagccctt cgacggcggc gttgacgttt ttagcggcca agagtttttc atcagccccg
     1141 tcttcatcgg tttcatacaa ggcagcctgg gccgaggcct tcttcagccc gtcgcctctt
     1201 tttttggcga tctgttgttc cggactgcgg tcgatgcggt aatatttctg gccgatggca
     1261 aagcggaacg agaccgacgt cggctcatcc ggcgtggcat attcgctgcg catataggag
     1321 ccggtgcgga tgtcgccgct ggtacttccg taaagggcgt agcagatggc atcgaggatg
     1381 gtcgtcttac cggagccggt cggtccgtag atgagaaata atttatggtc cttcaaccgg
     1441 cgaaagtcga cgatttccnn nnnnnnnnct tcaaaggaac cgctgacgat gcgcacgtcg
     1501 tggcggggcg taaattcatg aaagacggga tcggccttgc cttttccatc gaggtcgacg
     1561 acgacggccc cttttttctg tcgggcttcg ccgaaggaat atttcagcaa acttccggca
     1621 tagcggatgg tcggcagcgt aatctgctgc ggtccgtgaa gatggcccag ggccgtatag
     1681 gaaaaggacg tgaacaaatc gcttccgacc tggtcactgc cgccgatgga caagggccgc
     1741 tccgagtcgc cggcaag
//
LOCUS       DAWDXZ010000399         1752 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11587_length_1752_cov_10.119623, whole genome shotgun
            sequence.
ACCESSION   DAWDXZ010000399 DAWDXZ010000000
VERSION     DAWDXZ010000399.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11587_length_1752_cov_10.119623"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          469 a          424 c          443 g          416 t
ORIGIN      
        1 tcatattttt ttgaatcgtg aataagccga gttgaccggc tttgcagccg aaaacctctg
       61 tcagctcgca tttgaaacgg agctgatgga ggttttcttt tgggaatctg tctgtctgaa
      121 gagaagggga agaccatatt tagaactgaa ctaaagtcaa tgaatatatt tcactgaatt
      181 aaattcattg actttagttc agcgtatatg atacaatgag ttcatcccaa aaagcttgag
      241 gtgatatgta tgactaatcg acagatggcg gctcttgaga cacggcgaaa actcctcgat
      301 gcggccaaga aaatcatctg tgaaaaaggg atggtcaaca cgtcagtggc tgaaattacc
      361 aaggcctgcg gcgtggccag cggtaccttt tacacccatt ttaaacgaaa agaagatatc
      421 atctatgcct tgagccgtga gatgtatcaa gaaattttag acaaggccaa ggcctttgac
      481 ggacctttca tggcccggtt ggtatttttt atgactaatt tttcaggcta tattgaaaag
      541 agcagcctga agctctgcca ggaatgggtg cgcaatacgg tcgatcccga tttggtcgac
      601 gacggagagg ataaacccaa ggtctacgtc gatatcgatg ccgtaaccgg tctcattctg
      661 gatggaatcg aacgaggcga ggtcaaagcc gacgcaccgg tcgctgtcct ggctcatacc
      721 ctggtcgacg tcctctacgg tcaaatgctc tgctgggata tgtccggcgg tgcgtacagc
      781 ctcgaatggc ggaccaaaga gttttgccgc atcttcctgc ctgggttgat ggcgccgtac
      841 ttggcgtaat gttataagaa aaacggaaag tttccttgtc tgttaacggc aggctgagtg
      901 ggagcttttc ctttactgta gatatttttt tagaaaggat ggatcatgat gaccacaaag
      961 attgaaagcg taaccttaaa tgatggcgta aaaattccgg cctttggttt cggtactttc
     1021 cagattccgg ctgacggcag cacctatcag gccgtaaagg aagcccttaa cttgggactg
     1081 taccacatcg atacggcagc ggcctatttc aatgaagccg aagtcggcaa ggccgttcgc
     1141 gacagtggga ttccccggga agacatcttc gtgaccagta aactctggct ccaggactat
     1201 ggctatgaag ccgctaagaa gggaatcgac acgtcccttc gcaaactcgg tttggactat
     1261 atcgacttat acctcatcca ccagccctac ggcgacgtcc ccggtgcctg gaaggccatg
     1321 gaagaagcga aaaaggccgg taagatccgc tccattgggg tcagcaacat gacgccgaag
     1381 atctggaact ccttcgtacc ccagttcgat acggtacctt cggtcaacca ggtcgaattt
     1441 catccctact tccagcagaa agaaatcaga gccatcatgg ccaaagataa ggtctatctc
     1501 gaagcctggg cgcccctcgg acagggcaac ccgcagctct ttagcgaacc aatcattact
     1561 aagattgctg aagctcacgg caaagatgcc ggccaagtca ttctgcgctt tgaataccag
     1621 gacggagcca tcatcttccc gaagacgacc aaaccggccc gcatgaagag caacatggac
     1681 atctttgatt ttgcccttac ggatgaagaa atgaaggcca tgcgcgccct cgacacgggt
     1741 aaaggccgtc ac
//
LOCUS       DAWDXZ010000400         1749 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11607_length_1749_cov_9.148760, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000400 DAWDXZ010000000
VERSION     DAWDXZ010000400.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11607_length_1749_cov_9.148760"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          367 a          457 c          485 g          440 t
ORIGIN      
        1 atgttgtcga tgaacaacaa gacgtccttg tgttccttat cgcggaagta ttcggccatg
       61 gtaagaccgg tcaggccgac gcgcatacgg gctcctggcg gttcattcat ctgaccgtag
      121 acgagggccg tcttgtcgat aacgccggat tccttcattt cgttccaaag gtcattgcct
      181 tcacgggtac gttcaccgac accggtgaag acggaataac cgccgtggcc cgtagcaacg
      241 ttgtgaatca attccatgat gaggactgtt ttaccgacgc cggcaccgcc gaagaggccg
      301 atcttaccac ccttagcata agggcagatg aggtcgacga ctttgatccc cgtttcaaag
      361 atttctgttt ccgtcgcctg gttttcgaat ttcggaaccg gacggtggat cggccagtaa
      421 tcatcggctt cgaccttggc gtcgactttg tcaacggtat tgccgaggac gttgaagata
      481 cggcctaaga cgcctttgcc gacagggacc ttgatcgggt cgccggtatt ttcggctgcc
      541 atgccgcgga cgaggccatc tgtggagtcc atggaaacgc agcggacgac gtcgtcacct
      601 aagtgctgca tggcttctac aatgaggtcg atcttctggt cgccttcacc gcccgtgata
      661 tggatggcgt tgtagatgga cggcaagtct gcttcggaag caaaccgcac gtcgacgacc
      721 gggccgatga cctggactac tttcccaatg ttattgctca tgaagaggtt ctcctctctt
      781 tcaagaattg taacagcagc gcgtgacgag aatcacgtct tatttcaagg cgttggcgcc
      841 acttacgatt tccgtcagtt cgttggtaat accagcctgg cggactttat tgtaggtaac
      901 agtcagtttt tcgatcagct caccggcgtt gtccgtagcc gacgtcatgg ccgtcatgcg
      961 ggcaccgagt tcactggcag ctgactgaag gagactgttg tagataaaca gatctaagta
     1021 ctggggcagc aaagaatcga ggacctggga tgcatcggga gcaaacaagt actcttcccg
     1081 cggcccctgg tcaccggcct gttcttcctg agaaataggc agcatcttca cgatttttgc
     1141 ttcctgggac aaggccgttt tgaagtgcgt atagatgaca tagacttcat cgacttcgcc
     1201 gtcgaggaac ttctgcgtcg ccaaatgggc gatttcctgg gcattgatga gctgcggctt
     1261 atccgaaaaa ccggaccatt tttcttccgg cgtaatatgc aggtgcttta agtactctgc
     1321 cggtttgcgg ccggagacga aaaggaccga cgcactgcgc ggcttatcct ttaagacgga
     1381 taccatggtc ttgttgatgt tggcgttgaa ggcaccggcc aggcccttgt cagccccgat
     1441 gatgatatag cacgtcttct tgacgtcatc gcgggactgg aacaaagggt tcccggaatc
     1501 gggggatgcc gccgacaagc ggcgcagcat gccttccatt ttttcggtat acggggcagt
     1561 agccagtgcc cgttcctgtg cacggcgcag tcgcacggaa gagaccatct tcatggcctt
     1621 ggtgatctgc tgagtattgg taacggattt tatccgtcga tttatttcgc gtgcactcgg
     1681 catagcttat tcctcctctt taaccagttc gtgagaagta ccgtactgct tgaggtattc
     1741 cgcaatggc
//
LOCUS       DAWDXZ010000401         1749 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11611_length_1749_cov_7.079693, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000401 DAWDXZ010000000
VERSION     DAWDXZ010000401.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11611_length_1749_cov_7.079693"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          417 a          512 c          457 g          363 t
ORIGIN      
        1 ctgcggtgcc ggccggtcca tgggtttcgt ctgcgcaccc catctcttcc aggatgtcat
       61 tttgaaatgc atgggccaga cgtcggatat gaacgtctat gatgaaaacc gtaaattgtt
      121 atataatgga cttaaagaaa tgggctatga ctgtgtgtat cccagcggcg cgttttattt
      181 attcgtcaag tcgcccgagg ctgatgccaa ggctttctcc gacaaggcta aagctttgaa
      241 cctcctcatc gtaccggcag acagctttgg ctgcccgggc tatgtacgga tttcctactg
      301 tgtcgaagct gacatgatta agcgctcctt cccggcattc cggcagctca tcgctcaata
      361 ccaagaaaag aggtaacaga ctatgaacgt atacgacgag tacaaagcga aatgctgcag
      421 tgccgaccag gccgtcaagc tggtaaaaga cggtgactgg gtcgattaca gcatgagctg
      481 tgcgtatccg acggccctcg acaaggcatt ggcccggcgc cgtgacgaat tacaggatat
      541 caaggtgcgc aacgccattt cctgccatcc cgtcgctatt ttggaagccg atccggagaa
      601 caagaccttt acgtacaacc tgtggcactg ctccggtgtc gaccggcagt atctcgatgc
      661 cggccgggct tatcacgagc cgatgctctt ccgcgactgc ggctcctact accgccgggg
      721 ctttgcgccg gtcaacgtgg ccatgattac cgtatcgcct atggataagc agggcaattt
      781 ctcctttggc ctcaccaacg gctgtcagca ggaaatgctc gatgcagcgg aacacatcat
      841 cgtcgaagtc aatcctaaga tgccgaccgt cttcggcatg ggcaacgacc acatccatat
      901 ctcgaacgtc gacgccgtca tcgaaagcga tgaacccgta gggcttgccc cgaaccgcga
      961 accggcagag atcgacaagg aaattgccgg caaaatcttc ccctatctcc atgacggcat
     1021 taccctccag ctcggtatcg gcggcatgcc caatgcctta ggcatcctca ttgccgaatc
     1081 ggacttaaag gatctcggga tgcatacgga actcatgagt gacggctatc tcaacctcta
     1141 taaggccggg aagatcacca atgataagaa acccttccag cggggaaaag gtgtattctc
     1201 catctgcatg ggctcgcagg agctgtacga cttcctggat cacaaccagg acatcctgtc
     1261 agcaccgatg tactacgtca acaacccgga aacgattcgc cagctcgacg atttcatctc
     1321 tatcaacagc tgcattgccg tcgacctcta cggacaggtc tgttccgaat cggccggcac
     1381 acggcacatc agcggtacgg gcggacagct cgacttcgtc accggtgctt atgctgccga
     1441 acacggcaag gccttcctgg ccatgcagtc cagccgcgtc gaccgcaagg gcaaacgcca
     1501 ttccaacatc ctgccgaaat ttacgggcgg cgacatcatc acgacgcccc ggacccaggc
     1561 cccgtacatc gtaacggagt acggcgttgc caacctgtcg ggcctggcca cgtggcagcg
     1621 ggccgaagcc ttgattgaca tcgcccatcc ggaagttcgg gaagaattga ttcaagccgc
     1681 cgaagaacag cgtatctggc ggagaagcaa caaacgataa agtagtgtag cttgccttgc
     1741 aagggcatt
//
LOCUS       DAWDXZ010000402         1748 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11614_length_1748_cov_9.480803, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000402 DAWDXZ010000000
VERSION     DAWDXZ010000402.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11614_length_1748_cov_9.480803"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          418 a          457 c          443 g          430 t
ORIGIN      
        1 gcggactcgt aacatgataa aattttttgg ggccgtcatc atcatcgtgg cagcctttgt
       61 tactttgatc aatccgcttg ccaactccat taaacagggc ctggacttac agggcggtac
      121 gcatatcgtc ttggaagctg aagatacgcc ggatgcaccg gttacggaag actccttgag
      181 ccgtgccgtc accatcgtcg aacgccgtat caatgaaatg ggcctgaccg aaccgcttgt
      241 tcagaaagaa ggggctcggc gaatcatcgt cgaactgccg ggtgaaaaaa atccggaaaa
      301 agccatcgaa acaatcggga aaacggcggt catggagttt aaagatgaaa gcggaacgac
      361 gcggatgacc gggagcgatt tgaaaacggc aaaagaacaa atcgaccagg gtcataaaaa
      421 cgtcgtcggc attgaattta ccgatgaagg ggctaaaaaa ttcgctgacc ttacggcatc
      481 gaatgtaggc cataaaatcg gtatctacct cgacggcgaa ctcttgacca accccgtcgt
      541 caacgaaccg attacgggcg gcaaggccgt catcaccggc agccagactt tggaagatgc
      601 caagaattta gccatcctcc ttcgttcggg cgccctgcct gttaaattga aagtcatgga
      661 agtccgcacc attggtccga gcctgggcct tgactcgaag ctcaagagtg aaaaggcttt
      721 tgccatcggc atcctgttga ttatggtctt tttaatcgtc atttaccgta tgtccggtat
      781 cgtagccaat atcgccctct tggtatacgt cctcatcctg ctgggtattt tgaagtacct
      841 cgatgcgacg ctgaccttgc cggggattgc cggtgttatc ctgtctatgg gtttcgccgt
      901 cgatgccaac atcctcatct ttgaacgatt taaagaagaa gtcttggtcg gcaagacatt
      961 gcgaacgtcg atggaagccg gcttccgccg ggctttcaat accatcctcg acgccaacgt
     1021 atcggtcatg atcgctgcca tcgtcctcat cgtcatgggc agcggcacgg tcaagggctt
     1081 tgccgtcaac ttggccttgg gcctggtcgt cagcatgttc acggctatcg tcgtcagccg
     1141 ttcgctcctg acctggttca tcaatacggg attgatcacc aatccctggt tctacgggtt
     1201 aaaccgtaaa ccgcctgaac atatgctgaa aaagggggga cagaaatgag ttttgatatt
     1261 atcaaacatc gcacgtggtg gttcatctta tcgtctgtcc tggtccttgc cagcctgatt
     1321 tctatcttta tcaatggatt taacttcggt atcgattata ccggcggtac catcctcgac
     1381 atgcagttca ataaggccgt cagtgtttcg gaagttcgtc aggtcatcga tcaatcgaat
     1441 atggacctgg gcaatacggt catccagctg accggcgtta ccgaccagga ttcgtcgaca
     1501 gacgtcatgc tgcgcatgcg caacctgtcc agtgacgaaa ccaaggccgt gtccgaaaaa
     1561 ttgagcacct ctttgggcgg tgccgaagtc aaacggaccg aatcggtcgg tgccgtcatc
     1621 ggctcggaag tcacgaaaaa tgccgttacc agcctggctg tggccttcat tgccttagcg
     1681 gcctatattt ccttccgctt cgagtataag atcgccattt cggcccttat ttccatcgta
     1741 cacgattt
//
LOCUS       DAWDXZ010000403         1748 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11616_length_1748_cov_7.163024, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000403 DAWDXZ010000000
VERSION     DAWDXZ010000403.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11616_length_1748_cov_7.163024"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1298..1307
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          374 a          491 c          432 g          441 t
ORIGIN      
        1 gtctccggta aacagaaagc ccatcgtcac cgccgtacaa ggcaagttcc ggatcatggc
       61 gcacttcagg ctgaagatct gccatatcct gagccgtcag ataaggcgga ttcgacacca
      121 gccagtcaca ggccatggcc gggacctccg tgaaaaggtc actttcaagc caggttacgc
      181 ggtcgctaag acccaaggtt tggccattct cggcggccac ggccaaggcc ttcggggaaa
      241 tatcgacacc aaatcccgtc gcctccggta agtacgacaa gagactgaga acaatggcac
      301 cacttcccgt gccgatgtcg atgacgacag gctttgttcc cttagccgcc gctatcacgg
      361 tttctacgag catctccgtt tccggccgcg ggatgagcac atcctggctg accttaaaat
      421 caagtcccat aaaagcctgg tttcccaaga tatgggcgac ggataaacgg ctcccccgct
      481 ttttcaccaa ttcccgatac gccgccagtt cttcaccgtt catgggctca tcgtaatggg
      541 catataaata aatccgctct ttttttaaga catgggccaa gagaatctct gcatccagac
      601 gcggtgaatc gatgcctttt tctgcaaagt actgctgagt ccattgcagc aaggcaccga
      661 tggtccaaat cttattcatt ttctgcttct ttcatcagtt cagctcggcg agtttcagcc
      721 agagcctgaa taaattcgtc gatatcaccg tttaagacgt tatcgagttt gtacaaagtc
      781 aggttgatac ggtggtccgt tacccggccc tgggggaagt tgtacgtgcg gattcgttca
      841 ctacgatcac ccgtaccgac ctggctgcgc cgttcttccg tcagttcggc gtccgctttc
      901 tgctgagcct gatcgaggag acgggctttc aagacgcgaa gagctttggc cctattttcc
      961 agctgcgacc gttcgtcctg acattcgacg accatgcccg tcggaatatg ggtcatgcgg
     1021 acggcagaac tcgtcttatt gacgtgctga ccgccggcac cggaggcacg gaaataatcg
     1081 acgcggacat cgtccatatt gagatcgatt tcaacgtctt ctgcttccgg cagaacggca
     1141 acagtcgccg ttgacgtgtg gatacggccg ccggcttcgg taacggggac acgctgaacg
     1201 cggtggacgc ccgattcata tttcagcttg ccaaagacac cggatccttc gatagagaat
     1261 acgacttctt taaaaccgcc caattccggt tcgttagnnn nnnnnnngtg cagctgatct
     1321 tcaaggtcgg cctgttcttt tacaaggccg ttcatttctt ccttagccat ggccatgagg
     1381 tcatcatcgc tgtcggggtc gttcaagatt tcctgatccc cttctatcgt atgggtgatg
     1441 tccttatact tgcggaacgt ttcgacaagg ggcatgagcg acgcatgttc cttcgtcagc
     1501 ttctgccatt cttcctgacg ggcaatgacc gcagggtcgc tgatctgctg ttccaggtcc
     1561 atgaatttat tctccacggc ctgtacttta tccataaatg agctcataat tctctcctta
     1621 ctttatctct gtaacatcgc cgtcgtcagc cggtttcgca gccttcatag cttcgatggc
     1681 aacttctatc tggtcgggat gcggttcacg ggtcgtcaga tattgaagcc acagacccgg
     1741 tcggaata
//
LOCUS       DAWDXZ010000404         1735 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11701_length_1735_cov_4.872024, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000404 DAWDXZ010000000
VERSION     DAWDXZ010000404.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11701_length_1735_cov_4.872024"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          400 a          498 c          443 g          394 t
ORIGIN      
        1 gaatttaaaa atgtatccaa ggtctacgac aatggttccg ttgccctcga ccaggtatcc
       61 cttcagatcg gtaagggcga attcgtcttc gtcgtcggcg ccagcggtgc cggcaagtcg
      121 acgctgatcc gcctcttgtc tcatgaagaa ctgccgacga aagggtctgt cttcgtgaac
      181 gatatcgaag tcaaccgcct ggccaagaag agcgtgccct acctgcgccg taaaatgggc
      241 atcgtcttcc aggacttccg cctcctgccg aataagacgg ctcaggaaaa tgtggccttt
      301 gccatggaag tcatcgaagc accgcgccgc gtcattcgcc gccgggtccg cgaagtcctc
      361 gatctggtcg gcctcatcgg caaagccgac gccctgccga aaaacctcag cggcggggaa
      421 cagcagcgcg tagccattgc cagagccatc gtcaatcggc cgctcctgct cattgccgac
      481 gaaccgacag ggaacctcga tcccgatacg tccgaggata ttatggaagt ctttaagaaa
      541 atcaaccaca tgggaacgac ggtcctcatg gtaacccatg acaagacctt agtcgacatg
      601 atgaataaac gcgtcatcga attggatgac ggcaaggtcg tccgtgacga acagcgagga
      661 ggatacgacg atgaaaatta gaaccatgca ctactacacc gtcgctgcct tccaatcttt
      721 ttggcgcaac cgcttcatgt cggtggcctc tattgcaacg gtcgccatat ccctgttcat
      781 cttgggcatc ttcatgaccc tcgtggccaa tctcgactat tttgctgaaa atctcgaaag
      841 ccaggttgaa atcacggtct acttgaaaga cggtgccacc caggctgaca tcgacagcgt
      901 cgcccgccgc cttcaggccc tgcccgacgt caagtcggtc acctttaccg ataagaatca
      961 ggccatggag attttaaagg aacgcatgaa ggaccaaccg ggtatcctcg acgccctgga
     1021 agggaagaac cccttgccga gctcttatga agtgaccttt actcagccgg aagccgtccg
     1081 gcggacggcg gacatcgtcg ccgattaccc ggaagttgaa gggacgcatt atggtcagga
     1141 aatcattgaa cagctcttta agatcacgac catcatccgt tggggcggca ttgccctcat
     1201 cgtcttcttg accctggcca tgttgttcat catttccaat acgatccgcc tgaccgtctt
     1261 tgcccggcgt aaagaaatcg ccatcatgaa atacgtcggg gcgacgaatt ggttcatccg
     1321 ctggcccttc ctcttagaag gtttattcct gggctttatc ggcggcgtca ttgccgacat
     1381 cgccctttat cagttctacg actttgccgt cgcgtcgatc catgagtccc tggccttctt
     1441 gccgatggtc agcgtctttc cctttatgta ccgcacggca ggcgtcctct tgatcatcag
     1501 catggtcatc ggggccattg gcagtaccat ttctctgaaa cgttatatga aggtgtaata
     1561 tgtttaaaaa atacgttatc ccggttgtcg ttgccgcctt attgacgcct aacttcgccg
     1621 tcctggccga agatgaagac ttacagaatc agctgtcgga cgtccagacc cggatggcgc
     1681 aggaaagcga gaagatggcc caggccgaag ctgtcatcgg cagtgtcaat gacaa
//
LOCUS       DAWDXZ010000405         1727 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11757_length_1727_cov_6.974282, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000405 DAWDXZ010000000
VERSION     DAWDXZ010000405.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11757_length_1727_cov_6.974282"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          451 a          450 c          387 g          439 t
ORIGIN      
        1 aactgaaccc gtacgaatag aaacggggct agtctatcca tattcccggc tctgtcaaac
       61 gctatcaatg aaaaaaagga agctaaacca gcatccagat ccggacattc cataatctgc
      121 ggatatctcg tgcggagttc tttgaataag ccttcattct gccagtaatc gcctttcatc
      181 aattccagaa tatcctgaaa gaagctgtgt tccttcaaag cttcacccaa gcgaatacga
      241 tcagcctcca cagctgccga caaagaaagt gtcgggagcc aggcatcaaa agcagcctga
      301 agatacgctg cttcatcgtc agcttcggat aatgctgaaa gtgtttttat cgcttgagca
      361 tctggcactg tctgatacac gcgttcctgt cctgctaaaa attcagcagg gctgagtcgg
      421 tcttccgtaa tcatggcttt ttcatcgaag gcctcaccaa aaacctggct ggcataactg
      481 cggatggcgt ccctatttcc ttggccaccc atagttgcag aggtagcgac acagcaaaga
      541 tatccctgct gtgtataaag gcgtgatttc aagcgccgca gcaaacaggc caggtccgtc
      601 ccttgcgctc catcaaaggt atggaattca tcgacgacga tgaagcgcag cgtatccggc
      661 cgattatact gccaaagccc cgcatccttc gggcgcacta agagatagtc cagcatctta
      721 tagtttgtca gcaggatatc tggcggattg ttaatcatcg tatcgtgatc ggttataaca
      781 ccaagaatat tctgttcctt atccggagcg gacatgcaga tggccccttt gctgctgcca
      841 ccgacgtaca tgcctaccgt aatgccagct tgagttaaag caggactagc tgcaatttct
      901 ttagcaaaac ggctggcctg gtccgtagcc agcgcattca tcgggtaaat gatcagcgcc
      961 ttgatgcccc gttcatgacg atgctgaaaa caatattcta gcacgggata catgaagcat
     1021 tccgtcttac cggaccccgt ccccgttgca atcaaggtcg acttgccgcc ttcgcaaact
     1081 aaccgctgaa agctcttctc ctgatgtaca tagggcgtat agggaagtgt tacgccttcg
     1141 aaataatccg gtctagattt agcagtacga aacggtagct gcacagcgat atacgggtcg
     1201 tgtgtgacgg catcgccctg tttaaacatc gaagcgatag acccggcaaa gggctcattc
     1261 gtcataggaa atgtcgtttc aatataatct cttatgccct gctgcagctg tttagcaagt
     1321 atagaaggca gcatcgtaat cacttctctc tacgtatggt tacatacgaa tatttttata
     1381 tttattagca aaaaaatccc aaaccgtccg ataatcctgg atgcggtcac atcgtataaa
     1441 cggcgcttca tattctatgg tccgttctcg cgggccgccg ggctgcgtat catctgtaaa
     1501 ggaatgaaca taccgctgac ccgccgagta attatggata gttttccatt cgtctttgtc
     1561 gacgccaaca tatttccgac cttgatcatc cttccccgtc atgccgttac ttatggtata
     1621 gacaatacgt ccgtgcgcat cataccaggt atcagcttca tagctctgca gcacggggaa
     1681 ctggatgcgg taaatcgtaa tcaactgttc caaagtcatg cccaaag
//
LOCUS       DAWDXZ010000406         1722 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11788_length_1722_cov_7.744451, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000406 DAWDXZ010000000
VERSION     DAWDXZ010000406.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11788_length_1722_cov_7.744451"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    628..637
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          386 a          462 c          480 g          384 t
ORIGIN      
        1 gccatcgaaa tcgcccgcca attgcgcctg cgcgatatcg gcggcatcat catcatcgac
       61 ttcatcgaca tggcccagcc tttgaaacgc gatgagattt tgcagatttt gatccgtgaa
      121 acggccatgg actgcactaa gactcgggtt ctcggcatga cggccttgaa cctcgtcgaa
      181 atcacccgca aaaaagcccg ccaaagcttg taccaagtac agttcgatcc ctgcgacgtc
      241 tgcggcggat cgggatacct ctattcggct gaaacggtgg tcctgcagat catccgccgc
      301 ctgcgtcata tggcggcgaa tcaccagctt cgcggcgacg tcctcatcga agcccatccg
      361 gacgtcttgg ccctcttaaa ggataagaaa cgcaaggccg aatgggagcg ggaattgaag
      421 cggaccctct attttgaagc ctcagatcat cccaatcggg aagtattctc tattttatcg
      481 tatgcaccgt gataacaagc gggcttaggc gacctaaaag gagggaaggc catgtttggg
      541 aaaaaagtat tcatcctatc gctttgtttc gtgctgacgg ccttgtcggt tctggcgtcg
      601 tcgccgcagg aaggatatga acaggcannn nnnnnnntgg cctcttatga cgataagccc
      661 ggtgaagctg cccgtcagga attgatccgc cgcggttggg atattgactc tcggatacaa
      721 aaagacgata agtccgagtc gaaatttagc ctggctcgta agggcaggga cacgttcgtc
      781 gccgtcacag ggacggaaag tttggccgac gtcaaatccg acttgaatct ccactcgatt
      841 ccttataaag agggggatac gggcgatttg cggaaagtcc atgccggatt ttcctattat
      901 acgacgagcc tcctcgatac cccgtataag gggacgacgc tgaaacaggc tttaaaagcc
      961 gacgcggcat cgcaaaacgt gactttgacc ggccacagcc tcggcggggc agcggccctc
     1021 ttggcggcag cccgcctcta tgatgaaggc ctgccgcaga ttcaggttgt cactttcggt
     1081 gcgccggcag tgggcaattc agcctttaat gagacgtatg aacccctcct tcacgtagac
     1141 cgcatcgtca tggccggtga tcccgtgaag ggcattttac agaccgtcga tgggacatac
     1201 agccaatttt ccaaggaaac ggtgtgggac agtgcgccgt cggtgcagcg ctttgcccat
     1261 gacattgtcg gctatgccga tgcggctttg cgccattatt acgatgctaa aggggcctat
     1321 gaagcctctt tggggcatcc cttggcggcc gatgaggtcc gggcggcgac tccttctgcg
     1381 tgggtcctgc cactggccgt gaccgtcgat accgctcttt ccggcgatgt gccgtatatg
     1441 aggcaggctg ccgacaatca gttgacctat cgcatccatc ctttatacgg ggcttctgga
     1501 aaatccttgg cagagaattt gaaagcggct cgggataagg gctgtgaagc cgttttagtc
     1561 cgccgcctta cgggaaaacg agcgaaagac aaggattatg attttgtcat gacctatgaa
     1621 gaagtgtggt acgacgtaca gagcggcgcc gtccgttcgg ccttttccat gaccatgaat
     1681 accgaaaaga tgacgcccat cttggcgctt ctggccatgg cc
//
LOCUS       DAWDXZ010000407         1721 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11795_length_1721_cov_11.105042, whole genome shotgun
            sequence.
ACCESSION   DAWDXZ010000407 DAWDXZ010000000
VERSION     DAWDXZ010000407.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11795_length_1721_cov_11.105042"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    820..829
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          396 a          539 c          337 g          439 t
ORIGIN      
        1 tgctgcctga atatcgcgga actgaatggc ctgttccgga cagagttcga ccatttcctg
       61 agcctctgcg agaaccttgg ggtcctcgat cttcccgtta tgttttacgc gggccttgcc
      121 gtccgagcct tcggcaatat agtccgaaaa agcaaggcac gtcccacatc ccgtgcattt
      181 ttccgcatcg atttttaatc gtttcatcgt cgtcatctcc ttaaccgatc tttttcacag
      241 cttccgtgta tgccttcccc agtcggacgg tcgtcatgat ttcttgggca agctgcccgt
      301 ctcttgttac caccggcgca tcgacaattt ttttcaaagc atcgactaca ttcatgcagg
      361 tcatcaaaac gtcccgattc tcccgggtgt agcaatccgg atcactgcct cgtgcttcga
      421 tcaaggcctc agacgtccga atcgttttgg ccagcagcag attcaggcct gcgatgaccg
      481 aagaaatcgt ttcacactga ccgatcatga tatcgatggc tttggcgata ttttccgcct
      541 gatcatagac cacgtctgcc gcccgcagct gcttcaccga ctcagcgcga aactgctcgg
      601 cgtcgtctaa cagcgaccgg cccaacccgc ctttcaagag ggacatcatg acctcggaat
      661 cggtacattg gcccttggga atgatggcca tccgctgcag accatcgacg tattcttttt
      721 tcaacgggat aagggctaat gcccccttcc gtttctcggc ctggaaatca atttcgataa
      781 tcttctgata gagatccaca aagcggcgaa aattagaaan nnnnnnnnng cctctaattc
      841 ctgcgatttc tgcctcacca acagccgggc ctccgtttct cgttgaaagg ccttcatcat
      901 gcgtcgccgc gaatcttcgt ccatgttgca ggccttattc aagtcataaa taaatttccc
      961 tactatcagc gccgcgattg gccacatggc cccaacctcc ttttttatca tgcccttcac
     1021 gctaaagggt aaagcctttt cgatccttgg aatcaaaata tcgatcaaag gcatctcgat
     1081 tccgaaagca acattcctcg tcaaaataag ctaagattcg atccaaaccg gcaaaaatct
     1141 catcgacatc gtcggctaag gccgattcca gcatttgctc aaatcccaaa tggaaagact
     1201 catattggcg ccccttttcc gcttcatcca cggcgtccag ccatttctcc agctcttcca
     1261 gcacgacttc catggcctcc gtcaagcgct gaaaggaagc caaatcctct tcagacgcgg
     1321 tttccacgaa atcatgaatc gcagccacaa gatcttccat caagtcgtcc tctgcaccgc
     1381 gctccatgcc ggcgtcatga aagttcccat cgagaaccct caaatggcct tcacgcggct
     1441 gcccacttcc atccgaagta cgctctttcg tatctcccac gatatgcagg gtaaccatct
     1501 ccccctgtgt cttgtcagta cccttcaaag gaatatgtct cttccgttcc atatccatag
     1561 aatcacctca tctcaaaata cacctctacc aatgagtccc catgcgcagc cttccgatac
     1621 ccatcgcgcc tctcaataat cggagacaac accttctccc gcatcatcgc cgacgcctaa
     1681 aggactcgct cctttctttc aattatacag aactgccctg a
//
LOCUS       DAWDXZ010000408         1721 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11796_length_1721_cov_7.361345, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000408 DAWDXZ010000000
VERSION     DAWDXZ010000408.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11796_length_1721_cov_7.361345"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    522..531
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1464..1473
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          324 a          434 c          464 g          479 t
ORIGIN      
        1 gcgacacttg tcagaggagt tcgttttttg ttaaatcgtc tgtttgcccg gttccggtgt
       61 aaagaccaga cgcggttagt aaaggcgcat ttgtccttca tggcgtcaaa gaaatcgcct
      121 tctttaaaga attcagcctt gccggtcaaa aggcatccca tgcccatctt gccctgcact
      181 tcgtggctcc cgagggagac ttcgatgtac ggattgtcat ggacgttctt ttgggaatcc
      241 tcctttacgg attaccgacc ttggccagca tatcctgaac ggcctgatat tcactgtcag
      301 cggcaaaatc gtgttgactc ttccccttta cgtcatagcg cagccaaacc ttgccgtcgg
      361 ccatgacctt acagtctttc acggaaaaga cgacgggctg gtcgtcagaa aaaccgggcc
      421 gggccatgaa caaggtctgc gtgtcggcac tgccgtcggc tgccggggcg atgacgacgc
      481 tcacttcgtc gcataagccc tgttggagga agctccagtt gnnnnnnnnn ngtgtaaaac
      541 gaatcttctt cagcgtttgt ttcttcgatc caaaggtact tccccatgat cttgccgtca
      601 agagacgtct ccatatggca gaagatgtag gggcgattgg ctttatccat gaaactgcct
      661 ccttcgaaag aaacgagatc ctatataata gaaatataaa tggcttagag gaaatccttg
      721 attttttccc gcttgcccca gttgcgaagc ttgctcaacg ccttgccttc aatctggcgg
      781 atacgttcac gggtgacgtt aaagtgctgc cctacttctt ctagggtccg cggccggccg
      841 tctttgaggc caaagcgaag gtagaggacc tttcgttcgc ggtccgtgag acacgagaag
      901 acgtcttcga tctgttcttt caagaggatg aaggacgctg cgtcatcggg ggcaacggct
      961 tccgagtctt cgatgaagtc gcctaagtgg gagtcttctt cttcgccgat tggcgtttcg
     1021 agggagaccg gttcctgggc gatcttcatg atttcccgaa cgcgttcgac ggggatatcc
     1081 atcttttcag cgatttcttc cggaaggggt tcgcggccct tgtcctggag gagctgacgg
     1141 gaaatgcgga tcagtttatt gatcgtttcg accatgtgga ccgggattcg aatggtccgg
     1201 gcctggtcgg caatggcgcg ggtaatggcc tggcggatcc accaagtagc ataggtactg
     1261 aatttatagc ccttgctgta gtcgaattta tcgacggctt tcaagaggcc cagattgcct
     1321 tcctgaatga ggtcgaggaa cagcatgccg cggccgacgt agcgcttggc gatactgacg
     1381 acgagacgca ggttggcgtc ggtcagtttt ttcttggcaa tggtgccggc tttgatatct
     1441 tcttccgtcg catcgggagc gttnnnnnnn nnnatcttgt cgatttggtc cggcgtcaag
     1501 tcgtcttctt ccatgaggtt catgatttcc gtataggtca ggctgccctt cttctgcccc
     1561 gtttgcagga gttgctgtat gtgctgcagg gccagttccc gacgttcttt ttccgtcagg
     1621 gccggtttcg ccgacgcgtc tttcttcttc gtcgtcttgc ggcgtacggt cttctttgtc
     1681 gttttattcg tttcgttcat atcgtctgtc tttttattca c
//
LOCUS       DAWDXZ010000409         1716 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11829_length_1716_cov_5.086093, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000409 DAWDXZ010000000
VERSION     DAWDXZ010000409.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11829_length_1716_cov_5.086093"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    838..847
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          448 a          351 c          488 g          419 t
ORIGIN      
        1 cgctttttat tcaagataag ggcagtgttc tttctctatc ggcagggaag gatgtcgttg
       61 taaccaatgg cagcatgagt tttatcatag agcttaaaaa ggagggaacg gcgaatatac
      121 atgctgatca cgatgtgata ttgaatcagg agtatggtca gaatccaaag tacggtacga
      181 atcagatgat tcacgcggag aatcagagtc aagtgagcct atctgccgat aatgatatcc
      241 acttcaataa taaaggtgtg aatcgtgttg tatttggtaa gaataaggca gaagtcaatt
      301 tggtggccgg gaacgatatt atctataata atcagaagga aaatgcgaac cctgttcatg
      361 ttttagaatc tggaagcaag gcctctatca agtccggaca tgatattatt ctgaaagatg
      421 ttccgtcggt aaacatgttt gaccttaaag agggggcgtc tgtcacggta ggagccggaa
      481 atgatatttc cgtcagtacc aagagcggac aagctgtcag tctgattgat accgatgaga
      541 gcagtacggc tgatatcacg gcaggtcacg acatgacctt ggatatcggt gttgcagcgc
      601 ctgtcgtcca tgccaagggc acggtcaatt tggaagccgg caacgatatg actctttcga
      661 cgaccggagc agatgataga aatccgaaaa ttgccaatct gtatgccgat ggcgaaaagg
      721 ctgtgattac ggctaaggct accgaaggga cgctgactat ctctaataac ggggctgttg
      781 gggtatatgc taataatggc ggtaaaatta ccctcggagg aaaggttgac atggatannn
      841 nnnnnnntgg atacgggaga tggcgccgtg gttatcaatg gaggaacgat ttcctttacc
      901 ggcccatctc tgaagcttac gagtagtgac gcaggactta atgccgatga gggtacgatt
      961 acgcttgacg gggctgctga tgtggatgcc tctgacggag ctattgctca aaacggtgga
     1021 agccttctct ttaaagggcc tttgaccgta cgcagtacgg ataccggcct tgtagcttac
     1081 agagataata gtagtattac tgtcgaaggt gaagctgtcc tgaatggctt agacggagtc
     1141 attgctaaaa atggaggaac gattcttttt aacggacctt tgaccatcaa tagtacggcg
     1201 acgggactta cggctgatga gggcagtatc actgtcgaag gtgaagctgt cctgaatggg
     1261 gcttacggag tccttgctga atatggcgga acgattctct ttaacgggcc tttgacgctt
     1321 aagagcagtg cagcgggact tacagccgac ggtagcaaca tcaccgtcgg tggtgacgcc
     1381 gttttggaca cggcaaacgg ggctgtggct gttaatgaag gaaccattct tttcaatgga
     1441 cctttgacga ttcgcagtac cggcacggct ttacatgctg tcgacggcgg cgtcatcgag
     1501 gcgaccgcag aggattcggc taaatatatt gaaggccata tcgtggccga cggcggcact
     1561 gttttgttgg aattgaataa ggccggctcg tacctgaccg ggacgacgac cgtgtctgat
     1621 ggcgcagccc tttctcgttc ctcttatcgc aatcgagccg cagacggcac cgatgaaagt
     1681 gcgggaacgc tggacttgaa tcttgggacg ggaacc
//
LOCUS       DAWDXZ010000410         1712 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11851_length_1712_cov_10.324080, whole genome shotgun
            sequence.
ACCESSION   DAWDXZ010000410 DAWDXZ010000000
VERSION     DAWDXZ010000410.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11851_length_1712_cov_10.324080"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          421 a          477 c          404 g          410 t
ORIGIN      
        1 catgccatcg ctttcggcat ggaccgtgag cagtcctcca tgggatttca tcaccggcaa
       61 aattctcatt aaatcagcgt ctcccatggg gtaaccgtac gtcgtatagg ccttaaaact
      121 cggaaagcct tcttcaatca gttcggacaa ctcacgtaag accgcctcat cgacatgttg
      181 gaatacacca tggaaactat agtcgacggg acacgattcc gccagcaatt tatactgctc
      241 aaaaggatat ctcagcgaac agccttcagg gccaaaggcc atgtggtcga tgatagtcgt
      301 cgtcccgccg caggctgcag ccacaccacc gctgtaaaag tcgtcacagg ctctatactg
      361 aggcgactgc tgcaaatcca tgtgggtgtg cggatcgata ccgccggggc agacatacat
      421 cccatcagca tcgatgactt cttcactgcc acgatgaagg cctccgccga tagaccagat
      481 tttcccatcg cgaagggtga tatccgcttg aaagtgttct tcgccaaagc aaaccgttcc
      541 acctttgatg acataatcca tgatgtttcc ttcttcctta cttcagtaat agataatcgt
      601 aatctttaac gacagccttc atgaacccgc gaagttcttc ggggatggtg tcttcgtccg
      661 aacggagcgt cgtcgtcaaa atccgcccat cgagccggac cttacagaat ccgctttttt
      721 tatcgatgac ggcaaaacct tggttttcac tgttcatgaa atcgtcttca ttggcaaaca
      781 aggtgaattt atccatgtac ggcgccccgc cccagggaca gaaggtccgg cagtttccgc
      841 attcgttgca catagcatcg acgtggagaa tctgccgctg caaatggccc ggaacttcaa
      901 tggcgacgtt ggctcgattg ggacagactt cggtacagac ctcacagatg ctgtcacagt
      961 ggagacaacg tccatcacag ccggcagccg gcggcggcgc taaaaatcct cgtgcatcgt
     1021 aaatggcgtt tacatccggc agcagcagcg tatcgacgct ggtaagtcgt cctaaaatct
     1081 gttcagcggc ctgctttccg tcggcaatgc attctacgac agacgtcgtc tttccgtaat
     1141 ggccatcacc gatgacggtc aatccgtcga tgtcaccggc agccgatgtg ccgattttcc
     1201 ccttcacacc gcaagcggct aagacgtaat caaaagagtc caattcatcg agggaggaaa
     1261 tgacattgtc ggagacgata cgcaccccga gtttttgaat caatgccacg tctttatcga
     1321 tggcttccgc tgaaatctgg tttttctttt cacagaggaa ggtccgtacc atgccgcccg
     1381 gtgccggatt gttatcgaat acagtgaccg ttatgcctcc gcgggctaag aagtaagctg
     1441 cagacagacc tgccggacca ccgccgatga cggctgccgt tttaccgatc ggcaccggag
     1501 ccgaaagaga ttccatgacg ccgtcaaaac ctttttcagc agctatcagc ttattgcgcc
     1561 gaatttcgac agcctcttca tagaagtttc gcgtacagct gttcatacac gtatggtaac
     1621 agaccgtccc ggtaataaaa ggcaggggat tcttttccat gataatctga agggcttcca
     1681 catattttcc ctcagctact aaatccatgt aa
//
LOCUS       DAWDXZ010000411         1702 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11931_length_1702_cov_7.894961, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000411 DAWDXZ010000000
VERSION     DAWDXZ010000411.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11931_length_1702_cov_7.894961"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          352 a          429 c          484 g          437 t
ORIGIN      
        1 ttcgttgaca gtcttctatt tgtcatgcta tagtaaaaac gatataatat aatgaatagg
       61 tcaggtgtct cagacttaat agagaagccg gttcaatgcc ggcgcggtcc cgccgctgtg
      121 aggtggagcc aggctgcatg aatgtcactg tacgttttgt atgggaaggc gcagctttgg
      181 ccatgaaacc aagccagaag aactgcctga cggataatca ccgatagacc tgcgagcgat
      241 gggaaggaga ttgtacggag catattcggg gactgtcgtc tatacgacgg tccttttatc
      301 tttgcagccc ttatcttgca ggtccttaca ggaaaggggt tcattcgtac catggaagcg
      361 atgatcaatg gttttcagtt gtttcagcaa ggcggcccgg tcatgtatct gttattggcc
      421 tgttcgctct ttgcggtgta tattggcatt gagagggcca tgttttttac ccagatggat
      481 gccggcaatg cttttgccaa tcgtttttat acctatatga aagtccataa gacggaagcg
      541 gcgcgctccc ttgcagccga cagttccggc gggctgcccc tcatgttgac cgaagttttt
      601 gacggtcagg gagggactga atggaaagcc tttgtcgaaa tgcagtcggg gatcttcgtg
      661 tcccgtcttc gaagtcacct ctattatttg agcgtcatcg tcaccttggc gcccctcttg
      721 ggtcttctcg ggacgattag cggcatgatc cgcgccttta gtgtttttaa cgtccagtcc
      781 ggacaggcta tcgccattac gggcggtatc ggcgaagccc ttattgccac ggcgtttggc
      841 ctttgcgtcg ctgttttggc cttgatcatt cattcttatt ttacccagcg cctcgaccgg
      901 atcattaccg atatggagct gtgtttttcg gcgatggaaa cgatggacca gaaagtaggg
      961 cagtgagatg cggctacgtc aattacggga ttttgaaaag ccttccatca tgatcattcc
     1021 catgatcgat atcatgtttt tcctcttggt cttcttcatg atgtcgtcct tatccatggt
     1081 ggacatgcac agtttaggcg tccagttgcc cgtggcagcc gaggcaaaag acgcgccgtc
     1141 atcccagtat gccgtcacct tgaaaaaaga cggtaccctg tggcttaacg gcaaggccgt
     1201 cgatcgcgag gcactcatgg cttcggcccg ggctcggcaa ggggaagaca aggctttcag
     1261 cgtcatcctc tatgccgacc aagacgtcga ttacggccgt gtcatggccg tgttggatgc
     1321 ctttaagaat gtcggtgtga accgcatcgg cctcgtcgcc gacagtgggg ctcagccgtg
     1381 aggtggggga agaagaaggg gcgtattgct gccggatttt ccgtctccct ccacgccgcc
     1441 gtctatgtcg gcctggctgc cggcggtttc tttacggtcc ttcagcacta ttcgcgtcag
     1501 cccaatgtaa ccgacgtcat gatttataat gccggagata tggccggtgc cggcgggaac
     1561 gatgccgatg ggatggaccc tgtcagtcgt gacgggggcg gcgattacga tgaacaagaa
     1621 gtcgtatcct cgacgtcgga taaggctccg aacgaagaag gaacgccaac tgccgacgcg
     1681 acgtccctga cgccggacgc cc
//
LOCUS       DAWDXZ010000412         1696 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11970_length_1696_cov_7.725777, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000412 DAWDXZ010000000
VERSION     DAWDXZ010000412.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11970_length_1696_cov_7.725777"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1121..1130
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          431 a          425 c          425 g          405 t
ORIGIN      
        1 ttctccgaag aagctcagcg ctttggcaag gtcctgcgag ttcatattgc catcgatacg
       61 ggcatgagcc gcgtcggctt tcccgtcaat gaagccagcg tcgccgacat caagaccttg
      121 gcagccctgc ccaatatcga agtccgcggt atctttaccc actttgccgt atcggactgt
      181 gaggataaga cctttacgcg cgaacaatac ggccattttc gctggatgtg taagcgtatg
      241 gaagaagaag gcattgacat tgccctgcgc cactgctgca acagtgcggc cgtcctcgag
      301 cttcccgaat attactgcga catggtgcgg ccgggtatca tcatgtatgg ctgtgaaccg
      361 tcgccggaaa tcgatattac gccctacgac ctgcggccgg tcatgtccct tcgctgctgt
      421 atcgcccacg tcaaactcat cgatgccgga gcgaccgtca gttatggccg gcactatacg
      481 gcaccggacc gccgtaaaat cgcgaccctt cccgtaggct atgccgacgg ctattcccgc
      541 attttgtcgg gcaaggtcga cgtcctctat cacggacacc gcgtccctca ggtcgggacg
      601 atctgcatgg atcagtgcat gatcgacgtc agcggtgaag ccaacgttca cgccggagac
      661 gaagtcgtcc ttttcggtca gcaaggcgac agcttcattt ccatcgaaga aatcgcccgt
      721 gtctgcggta cgattaatta tgaaatcatg tgcaatttat cccgccgcgt tccccgtgtt
      781 tatatgaaaa acggcaaagc cgtcggccgg gaagagtatc tgtttgacaa gcccataagc
      841 gaatagataa gaaaaaatac tgttcctctc acgaggacag tatttttttt gttataaacc
      901 ttttatcatt cttgcaaaag tgatataata tatgtaaaga atgcatatta aagtgcaaaa
      961 ataatcatag gaaaaggcgg tgtattagta tgaattggaa cgacaaaaca gcggcaaatg
     1021 ttagggctat caagccatcg gggattcgca aattctttga tattgcagcc caggtcgaag
     1081 gcgtcctgtc cctcagtatc ggcgaaccgg attttgcggc nnnnnnnnnn tgcgatgaag
     1141 aaaagcctcg atgcgaaaga aacgagctat accggcaact ctggcatgat ggaacttcgt
     1201 gaaaccatca gcgaaacctt tgaaaaacgc tatggcgtca agtacgatcc gaaagatgaa
     1261 atcttggtta cggtcggcgt ttccgaaggc ttggcaatgg ctctgttggc tatgaccgaa
     1321 ccgggcgacg aagtcctcat tcctgatccg gcttatgtag cttatccggc agctgttgaa
     1381 ttagcaggcg ggaaagccgt attcgtaccg acttatccgg aagatgattt taaactgagc
     1441 gtggctgctc tggaaaaagt cgttacgccg aagacgaaag ctttggtcat cggctatccg
     1501 aacaacccga ccggtacggt tatgacggcc gaagaattga tgcctattgc tgaattcgtt
     1561 aaaaaacacg atctcatcgt catggctgac gaaatttact gcgaactcat gtacggcgat
     1621 gctaaattta cgacctttgc taaattaccg gacatgcgcg aacggaccat cgtatttaac
     1681 ggtttctcca aggctc
//
LOCUS       DAWDXZ010000413         1695 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_11979_length_1695_cov_8.412805, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000413 DAWDXZ010000000
VERSION     DAWDXZ010000413.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11979_length_1695_cov_8.412805"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          454 a          412 c          386 g          443 t
ORIGIN      
        1 gagtacctga tgacttcggt tatcaggtac tcgtcttttt atgtgaaatt atatcacgtt
       61 ttgtctcaca ttgcctgcga gtattcagcg tatcccctcg ctcccctcat tcctcctttt
      121 gaaaactgaa tcgatggttt ccttccagcc ttatttgcgt atatccgtta cgacttgttt
      181 ataggcttca atgaggatgt tgaagtcgag gttatgggcg acgccgtcgt agccttgggc
      241 gatgtactcc ctggcctgtg ccgtcgtgcc ggtaaagatg tagcagagtt tttcggcatc
      301 gtgacaggct tttaggattc tatcgagggc ttctttaaag ccgggatttt caaattggcc
      361 cgggattccg aggcaggtcg aaagatcgta aggaccgatg aagatgccgt ccaggccctc
      421 gatttgaacg atatcttcga tttgttctaa ggcttctttc gtttcgcact gaggtatgac
      481 caagaggcgt tcgttgctgt tagcatagta ttcttccaat gtttctgtag ccgcccaagg
      541 ggcgttgcca aaaccggtcc ccctgccttt aatgaagcct ctttgaccga caggagcgta
      601 ttttgccagg gccacggcct tcttgaagtc gtcgatgtca cgcaaacaag gcaggatgat
      661 agcctgggct ccacagtccg ccgcccgctg gatatctcta tgatcgacgt tagccagacg
      721 cacgacaggg acgaggtgaa ccagttcggc agcccggatg aggcccatca tcgtttctgt
      781 atcaaagggg ccatgttccg tatcgatgat gacaaaatca aggcctgtat agccgagaca
      841 ttccacttcc gacatattcc ccatactgac aaaggtacca atggcagctt tatccgccgc
      901 tgctaaatca cgcaaacgat tcaaagacac aataaccgcc tccttactta ataaaaacta
      961 aatgtataat gtcttccggc acttagaaag tttaattcaa gatttcatct caaaagactt
     1021 tttccaacgt atgctcccta tgcttctatc ataaagtata cctgcacgaa atgccaactc
     1081 tctgaacaca aaaaccgtag cagcaacaaa ggcagctacg gtctgaatag cgcataaagg
     1141 ccggatactt cacagtctct gtgcgtttat cttcacatct caaattggtt gtcagggact
     1201 aaggcaccat ggatatcgat atggatgata cagattagat gtcatgaata aattgctgaa
     1261 agcttcttta tcagcctgca aactggctgt cgaggggatc ggctccgtat tgattcggac
     1321 cgggcgtccc tgctgcaaac agcaagtaca gtgccacgaa gaaatcgatg atagggatga
     1381 acatcaataa gtaaaaccaa ccgcttcggt caagatcgtg gcagcggcgg attcccagca
     1441 tcacggcact gacggtggca atcgccgata caggataagc caaataggga gtaatttgcg
     1501 ccaataaggt ccctaaaaca gaagcaagaa tcataacgac gagaattctg agaaaatagc
     1561 gcagtctgtt aagtcgtccg gtgtaggaaa agtatttttg tatcatgagt tctcctttta
     1621 actaaaaatt cccaagaaac gctagatact ccatatcaaa aaaactcgca tactgcctga
     1681 acagttagcc cctct
//
LOCUS       DAWDXZ010000414         1690 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12014_length_1690_cov_6.998165, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000414 DAWDXZ010000000
VERSION     DAWDXZ010000414.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12014_length_1690_cov_6.998165"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          413 a          453 c          358 g          466 t
ORIGIN      
        1 gtcggttacc aggtcgtaca tgatattcga attgatgcgg cccgtttcaa cgagcgggct
       61 ttcggtataa ccgatggtcc cgaagaacca gtccgtcgcc gctctgtcag cgatattttc
      121 tgccgaatct gttgtcactg ttgtttcaga cgtaatgcca atcgtgtaat catacaagct
      181 gcccttagtt acattttgct gagtaatggg actggtaaac acagcgtcgc tgttagtcaa
      241 ggtagcaaaa tagaggcggt catcaatgcc catggaatcc agatgcatgg tcgccggatc
      301 gctcactacc gtctgcgttc cggtcgcact ttccgtagcg acgatatagt cactgtgttc
      361 ggtcttttct ttggcgccgc cattactggt ccaatcgaga tccatggcga tcgtgcccgt
      421 accggacagg ttcttcgccg tgatgctggt accgctgcgc gttccatcag tcatattaac
      481 aaaggagtca ttgtctaatg tagtcaacgt gctgtcgccc gttacatccc agaaagcacc
      541 gtttctcaag gtaatattaa tgtgagagcc ttcatcgaca accggttctt cacagccgcc
      601 attgtcttca tcaccgcaat caccaggtcc gggctcaagc gggcaagtac tttcatctac
      661 aatttcctcg tccgggtact ctacatcacc acaatcacca ggtccggaaa tagccggaca
      721 agtaccttcg tcgccttcag aatcttcttc gccataaaaa taatgtttct taatctcgcg
      781 caattccgta aaaccggtga aggatgagtc agccgtgtca aagaggacat caatctgacc
      841 gttatgggct cgcatattgc cgattataac cttgtccacg ccttctgtaa gagactgtat
      901 ccgtcctgcc cattttgtat aaaaagcatt gtcttcggct tgaatcttaa gggcatcgtt
      961 gaaaacgata tcgctgtcaa gtctagctac agctccgttc ttagcctgaa gaatggtcag
     1021 aggcccatta aaagtaattt ttgatctgtg atcttcctca tactcggact cgtcatcggt
     1081 ttcctcccaa tcgtcgatat cggcttgcct catgacaata ttattattat taaaattggc
     1141 aacaggcctg ttttcagctt ggtcaattgt ctgtagcccg ttaaaatcca tttctgatgt
     1201 ttcttcgccc tcgtacggga aatcggtttg cgcccacgcg ccgataccga cgtttgacat
     1261 aactgtattt ttgttaaaat taatcttccc gccgtcttcc gaacgagcac cgataccgac
     1321 ctcgggcata atcgtattgc cgttaaaatt aatctttgaa aaacctactg cctgagccgc
     1381 aacagcgccc ttattatcga tggaaatcaa tccatccgtc gtcaaatcga tgttcccgga
     1441 ctcagcccac aaattcgctt gagaccaata tctgtaatcc gaaccggcat tgtgcaggta
     1501 aatcgagtcg ccggcgctca gcgtaacatc gctattgaag gcgtgcacca gcgtggggct
     1561 tatctccgac gataacttaa gagtacggtc cgcatcaaaa acaaaatttc catcgctagc
     1621 tatcaccata ctggcatcat cgaacaatcc tacagggtcc acttccttac cttgagccac
     1681 ccattcaata
//
LOCUS       DAWDXZ010000415         1685 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12052_length_1685_cov_6.450307, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000415 DAWDXZ010000000
VERSION     DAWDXZ010000415.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12052_length_1685_cov_6.450307"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    598..607
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1039..1048
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          424 a          460 c          426 g          355 t
ORIGIN      
        1 ggccgaccag gttgaagccg gcagcggact gcacctcatc cagagttttg aaccgtaccc
       61 gctctatgaa gccatgaccg accggggatt tgaatactat gtcgaagaag tacagcctca
      121 tgaataccac atctggttct atcgccgtga agccaaggcg gcatcgggga agaagccctt
      181 cggtccggca gccctcttga atttccccat catcgataag aatttgggcc gcattgccgt
      241 cgaattctgg gatgaaacgt ggaacgacga acgccattac ctaccctatg aaatacggct
      301 catgttgtcc ctggccaatg ccgtcggtgc cggccgccat cgccaggctg tgcgggaact
      361 ggtcaaagcc tatatccacg gcatcgattc tagggcttta gacgacgtat tcgaactcat
      421 tgcctggaat cagggcatcg gcttcttcag ttctgaaatc ggcccgtcgc ccttattcgg
      481 agcctataaa ttaatcaagc aggaagaaga aaaggggacc gatcgggaat ccattaagca
      541 cctcttgatg gaacagttcg gcgaaaagat tcagcaagtc aaagtatttt aagcgatnnn
      601 nnnnnnnggc gtaatattgt agtacagaca aggaagtgtt accatgctcc atcgcgatgc
      661 attgatcaat cgttttttga aatacgtctc ttttgatacc cagtcggacg aagaaaatga
      721 cgccgtttgc ccgagtacgc cgggccagct cgtcttttcc aaagccttag ccgaagaatt
      781 gaaaggcctc ggcctttccg acgtttccat cgatgaacac ggctacgtca tggccacctt
      841 gcctgctaac ggcgtcgacg gccccgtcgt cggcttcatt tctcacgtcg ataccagccc
      901 cgatgcctcg gggaaggaca tcaaggtcgt ccgcaaagat aattacgacg gcaaggatat
      961 cgtcttgaat gaagaaaaga acatcgtctt ttcgacggaa tccttcccgg aaatcctcaa
     1021 atataagggt caggacatnn nnnnnnnntt accttccacg gccgcagcgt ccataccggc
     1081 gatgccaagg gcaagatgaa aaacgccctg accatggccg ctgaatggca gcagctcctg
     1141 ccggccgggg aaaaaccgga atacaccgaa ggccatgaag gcttcttcca cgtctataaa
     1201 ctgaacggcg acgtcgaaac gtgcacgatg cacatcctca tccgcgacca cgaccggaaa
     1261 ctttttgaac agcgaaaggc cttcctgcgg cagatggctg ctctctttac cgataaatac
     1321 ggccaaggga cggtggacct cgacgaacgg gacgtctact ataacatgct ggaaaaaatc
     1381 gaagacggtc atatggacgt cgtcgacctg gctaagacgg ccatggaagc cgtcggcgtt
     1441 acgccggaag tcgctcccat ccgcggcggt accgacgggg ctcagctctc cttccgcggc
     1501 ctgccgtgcc ccaatctctt tacgggcggt gccaacttcc acggccgttt cgaatacctg
     1561 cccattgagt ctttggtcaa agcctgcgaa acggtcattg aaatcgccaa acgggcttat
     1621 acgttacaga aataggaggt accatgaaag aaattacgca agacttgtta cagggctttc
     1681 acaag
//
LOCUS       DAWDXZ010000416         1684 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12059_length_1684_cov_7.473297, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000416 DAWDXZ010000000
VERSION     DAWDXZ010000416.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12059_length_1684_cov_7.473297"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          460 a          429 c          467 g          328 t
ORIGIN      
        1 tacgacaaat cggttaacag ccgattgctc taccaactga gctattgagg aataactcat
       61 ttcggccgct ccgaccggaa caagtgttat tatactagaa ctccatagaa aaagcaagta
      121 ctttttttat aaatttcaca aacttcccct tcccgcttca cgaagggccg gatcgggacg
      181 gcccaggaag tgaaagtagg caatgaagca atagagcaag gccaacaatc cgatgccggc
      241 tccgacgtaa ccgatataag gcaagaagcc gtaggccatg gtccggccgc cgatccaggt
      301 tcccaatccg atgccgacgt tgaagatgcc ggaaaagatg gacatggcaa ccggcgtcgc
      361 tcccgacggg acctgtcgaa tgagttcggc ctggaaggtt acattaaaca aagtcgacga
      421 gcagcctaac atcaggcaga cggccagcat ccattcccat tgcgacgacg ccagctgcca
      481 caaggcgagc atcaggacca ggccgatgaa gcccaggcgg atgatgccgt agcggtagtg
      541 attgtaaaaa tgagagaaaa tatagctgcc catgaggccg ctgacgccga ggagcatgag
      601 cgtcgacgta atgccgtcag cactgaactg cgctaccagc ttcaagaaag gttcgatata
      661 gctgtaacca aaaccaacaa ggccacgacg gtgatgctga caaaggtcat gcgccaaccg
      721 gcataaaggc cgatgacccg ccccaaggga aggccgaaga tcatggctac ggacgagccg
      781 gtaatgatca tgctcagggc cagaggccga tgtaccctgg tgactaagcg ggccgccatg
      841 ggtgccgcaa tggaccagaa aacagagtgg gcacaggcga ccaggatacg ggctgcgacc
      901 agcatgggat agttgacggc aatccccgac aggaattgcc ctaacgcaaa gacgctgagc
      961 gtcgccaata agagtttccg cagttcaatg cggtagagca ccagcatcag cggcagggac
     1021 aataaggcca ccatccaggc gtagatggta atcatgatgc ccgtctggtc ttcggtcatc
     1081 ccgaaggact gggaaatatc gatgagcagc ccgatgggca tgaattccga tgtattgaaa
     1141 ataaaggccg atacggtaag ccctaagagg ggcagtacat attttaaagg aatagtgcga
     1201 agcatgagag tcctcctttt tgcaaaattg aacttcttta tggtactcgc cgtccctgta
     1261 aaagtcaaga ttgtttggta tgcaaaaaaa agaaacctgc catgagacag gtttctttaa
     1321 ggagagtaca gtcagtgtat tctaaccgga cgatcttaca tcatgccggg catgccgccc
     1381 ataccggccg gtgctgccgg agctgccgga gccggttccg gtttgtcagc aaccaaggtt
     1441 tccgtcgtga ggacgagagc tgcgatggaa gctgcattct gaagagccgt acgggttact
     1501 ttggccggat cgacgatacc ggctttaacc atatcgacat attcttcttt aagggcattg
     1561 aagcctacgc cgtcttcaga tgctttaacc tgagcgacga tgaccgagcc ttcgaggccg
     1621 gcgttttcag cgatctgacg gagcggagct tcaacagcgc gtttgacgag gttgataccg
     1681 gtct
//
LOCUS       DAWDXZ010000417         1682 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12072_length_1682_cov_6.226798, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000417 DAWDXZ010000000
VERSION     DAWDXZ010000417.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12072_length_1682_cov_6.226798"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          477 a          423 c          412 g          370 t
ORIGIN      
        1 tcgccctttc acggcgagaa catgggttca aatcccgtag gggtcaccac accgataaag
       61 agttgtctca ttgagatgac tctttatttt tttgccaata tgttacagaa tcagcacata
      121 tcgttaaaat atttttgata tttataattt agcataataa aaatctaatt atgtaaaatt
      181 aataagggaa aatccttaat atataaacat acgtctcttg taaaaagact tagaggacgt
      241 aactctcttg acgatagaca ggtgaaagcg tatagtaaag atagcaaaaa cgcattatta
      301 taaattaaaa ggagggacgc tgcctttgca gcaaacaaca tgagtgaaca acaagtaacc
      361 ctacaaatga tccaagaagc ccaggagagg ctgaaaggag tcgcccaaaa gacgggactg
      421 tcgtacagca attctgtcag tgatctggca ggctgtaaag tctttttaaa actggaaaat
      481 ctccagcgga ccggttcctt taaactgcgc ggtgcctata ataaggttgc atcgctgacg
      541 ccggcagaac gggaaaaagg cgtcattgca gcctcagccg gcaaccacgc ccaaggcgtc
      601 gccctggcag ccagtgaata cggctgtcaa tcggtcatct gcatgccgaa acatgcgccc
      661 ttgagtaaga ttgcggccac caaaggctat ggagccaacg tcgtccttca cggcgacttc
      721 ttcgatgaag cggcagctaa agccgttgaa ttgacagctg aacacggcta caccttcgtc
      781 catcccttca acgatccgga agtcattgcc ggccagggga cgattgccct tgaaatcatg
      841 gaacagctgt ccgatgtcga tgccatcata gcccccattg gcggcggcgg cctcatttcc
      901 ggcctggccg tagcggctaa gagcgtcaat ccgaaaatca aggtcatcgg cgtccagacg
      961 gaaaacatgc cgtccatgaa agtttccctt gaaaacggcc aagccattgc ctataacgga
     1021 aaagcaacct tagccgatgg gattgccgtc aaggtgcccg gcgatttgac cttctccatc
     1081 tgtcagaaat atgtcgatga agtcgtcact gtcgatgaat cggaaatcgc cagtgccatc
     1141 ctgatcctcc tcgaacgagg caagaccgtt gccgaagggg ccggtgccgt accggtcgca
     1201 gccctcatga acggcaagat ttccggcatc cgcaataaga aggtcgcagc cttggtaagc
     1261 ggcggcaaca tcgatgtcaa caacatgacc cgggtcatca atcaaggcct catccgctcg
     1321 cagcgcaaaa tctttttcca gaccatcatt cccgacgttc ccggcgaatt ggtcaagctc
     1381 ctgtcgatca ttgccgatac gagtgccaac gtcctctcca ttactcatga acggagccag
     1441 cacggcatca gcatgggtac gacggcggtt tcgctggaat tggaaacggc caatgaaaag
     1501 cacgttgaaa agctgatgaa tgtccttcgc gacaatcatt acttcgtcat gatgaaataa
     1561 ggaggaaatt gaaatggaag accataaaga aggggcgcag aacttagaac ggggcttaaa
     1621 gccccgtcat gttgaaatga tcgcccttgg cgggactatc ggcgtcggcc tgttcatggg
     1681 at
//
LOCUS       DAWDXZ010000418         1680 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12085_length_1680_cov_5.576000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000418 DAWDXZ010000000
VERSION     DAWDXZ010000418.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12085_length_1680_cov_5.576000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1387..1396
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          362 a          446 c          468 g          394 t
ORIGIN      
        1 attgtgaata ctgaccaagc ggtatgccag gagttcttcc gccttgaaga ggtggcggat
       61 ataggcccgc gaatagttgc ggcacgtata acactggcag ccctcttcaa tgggatgaaa
      121 atcatgagca tacttggcgt tgcgcacggt cagccgccct tcatgggtca tagccatgcc
      181 gttgcgggcc acgcgggtcg ggaagacgca gtcgaacatg tcgacgccgc gggcgacgcc
      241 ttcaagaaga caatcgggag tcccgacgcc catcagataa cgggccttgg tcttgggcag
      301 gtagggcgtt gtattctcta atatatcata cattatatcc ttagattcgc caacactcaa
      361 gccgccgatg ccgtagccgt caaaatccat gtcgaccaga tcgcggcagc tctgctgacg
      421 aagatccttg tacatcccgc cctgaacgat gccgaagagc ccctgtgtat cgctgtgatg
      481 atggtcgagg cagcgcttgg cccagcggct ggtccgttcc gtcgagagtt tggtgtattc
      541 ataatcggac ggatagggga tgcattcgtc aaaggccatg gcaatatcgg aatcgagggc
      601 catctgaatg tccatcgaca cttccggcga caagaatttt ttcgagccgt ccaagtggga
      661 acggaaggtg accccctctt cggttatttt gcgcatctga ccgaggctga agacctgaaa
      721 gccgccgctg tcggttaaga tggcctgctt ccagttcatg aacttatgaa gtccccccgc
      781 ttcttgaacc aggtcatggc ccggacggag gaacagatga tacgtattgc tgagaatgac
      841 ttgagccccc atgtcataga gctcttccgg cgtcagcgtc ttgaccgtcg cctgcgtacc
      901 gacaggcatg aacatgggcg tcttgaacgt accgtgaggc gtacgcagga ttccggcacg
      961 ggcgccgtcg cattcggcct gttgttcgta ggtaataacc atagattcct ccctaatgaa
     1021 tgaacatggc atcgccaaaa ctgaagaaac ggtatttttc cttgaccgcc gtttcatagg
     1081 ctttcaagat tttttcccgc gtcgacaagg cactgatgag catgagcagt gtcgattcag
     1141 gaagatggaa attggtgacc agggcatcga cgatgtggaa ggtaaagccc ggatagatga
     1201 agatattggt ccagccggaa ccggccttca aggtccccgt cgtaccggcg ctttcaaggg
     1261 tccggatact ggtcgtaccg acggcaatga cccgccggcc ttcggccttg gccttgttga
     1321 tcgtcgctgc cgcttcttcc gtaatgctgt aaaattcgct gtgcatttcg tgatcttcaa
     1381 tgttttnnnn nnnnnnctgc cgccgagccg cgttcattgg catagaccgt ctgatactga
     1441 ttggggtctt ctatcttttc gtggatatac ggcggaaggg gcatttcccc gatggaatcg
     1501 atgatatcgt cgaaattgcc gtcgtaggag aatttgatga tacggccgcc aaaatcggtc
     1561 cgatcttcga ccgtccccgt aaggccttcg gcaaattcaa gggtcgttcc cggcaaggcc
     1621 ttcttccccg gttttaccag gacttcccag cggtcgtcgc cgacgggagt caacagcagg
//
LOCUS       DAWDXZ010000419         1680 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12081_length_1680_cov_7.180923, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000419 DAWDXZ010000000
VERSION     DAWDXZ010000419.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12081_length_1680_cov_7.180923"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          486 a          421 c          341 g          432 t
ORIGIN      
        1 tgcgaatcgt catgggccga tactcgccct gcgtctccgc cttggcccga atgagtccgg
       61 cgccgatata gaggaccagc aggctgccaa cgcctaagat gaccatttct accttaggaa
      121 agagttccat cacccggccg ataccgaaga aacaggccag ggacaaggta atatcgaaaa
      181 aaaggacgat gagggccgtc aaaaaagccc gccgccgcgt attattcagt gccgaattga
      241 tgataaacat attttgcatg ccaatggggg ccacataggc caagcccaac gtcaggccct
      301 gcagaaaaaa gctcatagaa aaaactccct tgccaacata tagaagaata acatcatcga
      361 acgcaccaaa cggtgttaat tttttattat acaggatatc acaagggatg acaacaaaaa
      421 aacagcggcc attaccgaat tgtctcgagt ggcctaattg gtattgcaat taaccatatc
      481 gtcattgttt gaacataaat tttccataaa agctacgcca cctacgatag ccacatcaaa
      541 aaatgcccta tggtctccct ggcatcaaac agcaaaaagg gctgaccact tgctatcgca
      601 agtagtcagc cgtaaaaaac actttgacgg tcaccgctcg gggccaaaaa gtatttattc
      661 ctcgatatcg taaaaggtaa agccttgtgc ttctgcttgt tgtatcaatt tttgccgtaa
      721 cggaattgcc ttcttaccgg caatctgttt cgtaaaggaa tctacccgtc gattcgtatc
      781 acgcaaatcg taatactctt ccatttccgc atcgtaatct ttcaacagtt ccaaataaga
      841 atcaaatacc cgataagagt cctcaaatac gttggcttca cgaggcatac gcggctttaa
      901 ctgcggttcc tgattcggta tcccaatgcc gacccccact acggggaagg ttaattccgg
      961 caatttcaga agttcaatca tcttaggtgc attatttaag atactgccaa aataaaccgt
     1021 accgagacca ctaagttcag cagcggccac catattttga gcggctatag cggcatccgt
     1081 ccagccttga aagaaacgat cgctatcgcg ttcaccaaca ctttctaccc ctcgttcgcg
     1141 agcgatacgg gagttgcggt atgcatcgac gatgaaaata aacagctccg gtacccgtgc
     1201 tacatattcc tggttgcaaa cttctgcaat agcccctttc agttccggat tctcaatgtg
     1261 gatgatactg aaactttgca tccccatgct ggtagccgtt cttttggcta tatccaataa
     1321 taggctcaat tgctgcggcg gcataggatc ggatttaaac tcacgaatag tgcgatgatt
     1381 caataacacg tccataggat tcatgggatc agctccttta caaaatacgg cgagtacacg
     1441 attctattcc tggcacataa tatcgaactt ttttctacaa taacactaaa tatgcacctt
     1501 gtctagtcat taccatggtc tggcttcaat ggcttacgca aattggtccc ccgcttcatc
     1561 ctctaggtag agggctttcg ctgtatattc cttggtgctt gacgcaaaaa aacaggttct
     1621 ataataaatg ttggggtctt gtggacactc tagtttacaa gacccttgtt ctatatacaa
//
LOCUS       DAWDXZ010000420         1679 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12087_length_1679_cov_6.403325, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000420 DAWDXZ010000000
VERSION     DAWDXZ010000420.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12087_length_1679_cov_6.403325"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    878..1002
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          399 a          407 c          410 g          338 t
ORIGIN      
        1 aatggaattc agaccactca tggcagctga cttaaaaacg atggatgctc gtatttttta
       61 tccgtctgcc atgggaattt aaaggaggat actatggaat atcgtattga aagagactcg
      121 atgggagaga ttaaggtccc ggcagacaag ctctggggcg ctcagaccca gcggagcttt
      181 gaaaacttta aaatcgggac ggaaaagatt cctgccgaaa tggtcacggt ctttgcctat
      241 ctgaaaaaag cagccgccct gaccaataag gaactgggcg tcgtcgatgc cgataaggcc
      301 gatgccattg caatggcctg cgatgaaata ttggcaggga aactcgacgg caatttcccc
      361 ctggccgttt ggatgaccgg aagcgggacc cagttcaata tgaacgtcaa tgaagtcgta
      421 gcccatcggg cagcggctat ccttaaagag gcgggcaagg aacagcgcgt ccacccaaac
      481 gacgacgtca accattcaca gagttcgaat gataccttcc cgacgggcct tcacatggtc
      541 ggggttctgc tcatcgaaca gcagctgttt ccggctatgg atgccctcta tcaggccctg
      601 cgtgataagc aggaagcctt taaagacatc gtgaagatcg gccgcaccca cctgcaggat
      661 gcgactccct tgaccctggg acaagaattc agcggctggg cccgcatgat tgaacgcaat
      721 aaggaaatgc ttcgctgtgg cgtcgatttt ctccgtgacc tggctatggg tggcacggct
      781 gtcggtacgg gtattaactg tccgaaaggc tatgaccgcc gctttgcgga aatcgtcagt
      841 gaattgacgg ggacgacctt ccatacggca ccgaatannn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgccgtcc acggcatgct
     1021 gaaaggcctt gcctgcgacc tcatgaaaat cgccaatgac attcgctggc tgtcatcggg
     1081 accgcgctgc ggcatcggcg aaatccgcat tcccgaaaac gaaccgggca gctccatcat
     1141 gcccagcaag gtcaacccga cgcagtgtga agccgtgacc atggttgccg tccaggtcat
     1201 gggcaacgat gcgaccatgg gtatcgccgc cagccagggc aactttgaat tgaacgtctt
     1261 tatgccggtc attgcctaca accttatcca gtcgatccgc ttgttgtcgg acagcatgga
     1321 atccctgcgc aagcactgca tcgtcggcat cgagcccaat gaagaacgga tcgagaccaa
     1381 tctttacaat tctctgatcc tggtcactgg ccttgtaccc ttagtcggtt acgataaggc
     1441 ctgcaccatt gccaaaacgg cggcgaaaga aggcttgacc ctcaaagaat cagccctgaa
     1501 actgggatgg gttacggctg acgatttcga taaggccatg gatccagcga agctggccgg
     1561 tatccggtaa cagccctttg gcaatttcga tgcaaaatag aaaataattt gtactaaatc
     1621 tctccaaaac cttttgcact ttttgagaag tagtgataga atagatgtac caatatctc
//
LOCUS       DAWDXZ010000421         1670 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12157_length_1670_cov_5.523839, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000421 DAWDXZ010000000
VERSION     DAWDXZ010000421.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12157_length_1670_cov_5.523839"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          375 a          447 c          454 g          394 t
ORIGIN      
        1 ccgccgctcg aaaccggata ccccgtgctg cccgtcgccg tcgatacgat gaggccgtca
       61 ccgggatact gctgaataaa atgaccgttg atgctgaggt cgacgcggat caatttgccg
      121 acatttccgt ggccgatgac gatatcattt aaggcatcgg gaagagactg aatcataccg
      181 tcttcgtagc acagatcggc atggagcatc atccgttctt cgaggaaata ttttccggcc
      241 aggatatcgt ccagacgctg ttcgaggttc cgcgtttcga cttcatagag gaaccccaag
      301 gaaccgaggt tgatgccgca aatggggacg tctgccgaag caaatttatt ggccatctta
      361 agaatcgtcc cgtcgccgcc gagaaccatg gcgatatcga tggtgtcgta aatcgtcatt
      421 cgcggccgga aatggtcggc tgcaatttcc tgataggccg gctgacagct ctcactgacg
      481 taagccggca agtagtattc aagaccccgg gcttcacaaa tccggatgag ttctttgacg
      541 atcgctgcgc tttcgcgttt aacaaggttc ggaaaaatac cgatacgcat gagaaatcac
      601 ctacatttca tgagatttac cgacaatatc ttcgacgacg tcatcggtga tatcgttgtg
      661 acaaggttca tctttacggc tgtacaacag atattcgata ttgccgtcgg ccccctttat
      721 cggggaaaac gtgagcccca gcggcgacag cccgttttcg gcgatgccgt cgacgacgcg
      781 gcggatgacg tcacgatgga cggtgatatc gcggacgata ccacccttgc cgacgtgttc
      841 ccgcccggct tcaaactgag gttttataag ggataccatg acgccgcccg gtgccagaag
      901 gggcacgacg gccggcatga ttttgaggag ggaaataaag gagacgtcga tggaaatgaa
      961 gtccacgggc tcgccgatat cggaaacggt gacgttgcgg atattggtcc gttccatatt
     1021 gacaacgcgc tcatcggtcc tgagtttcca agccaactga ccgtagccga cgtcgacggc
     1081 aaagactttc acggcgccgc gctgaagggc acagtctgta aagccgcctg tcgaggcgcc
     1141 gatatccatc atcgtctttc ccgtaaggtc gatgggaaat atttccatcg ctttttctaa
     1201 tttcaggccg ccgcggccga cgtagggact gggattgcct ttgacggtga attctacgtc
     1261 gtcggccagt ttggtaccgg ccttatcgac gcgctgattg tcaacgaaaa ccaggccggc
     1321 catgatggta gccttggctt tttcccgact ttcagacaag ccgcggttga cgaggagtac
     1381 gtccagtctt tctttatgct gcatacgatc ttacctctcc tatcattaat ggacccgggt
     1441 cagcaggtgt tcgacgagtt ccttcaggaa ccaggccttg tcccctaagg gttcaagggc
     1501 cataacggct ttatcagccg catcgcgcgc cattttccgc gcttcttcga gggaaaatat
     1561 ggtgacatac gtcgccttgt cgtttttaag gtcgctgccg acaggttttc ccaaaagggc
     1621 ttcgtcgccg acgacatcga ggatatcatc ggtaatctgg aaggtcagtc
//
LOCUS       DAWDXZ010000422         1667 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12176_length_1667_cov_8.921216, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000422 DAWDXZ010000000
VERSION     DAWDXZ010000422.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12176_length_1667_cov_8.921216"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          368 a          499 c          374 g          426 t
ORIGIN      
        1 aggccatcga ggcgccaagg gaaataccgt gcaggccgat ggtcatctgc ggatcgcggt
       61 tttgcagcca ctgacaccac atcagtaagt catcgacttc gcttatcccc cattcggtgt
      121 ggtcaccttc actttcacca tggccccgca aatcgatgag cagaatgtta taacccaggc
      181 ggcggtattc atccatgtag ggcagacaca ttgttctatt ttggtacaaa ccatggatga
      241 ggataaccgt cttgtgtgag tcgcggcgat tttcaatata ggtcccgcgc aggatttttc
      301 cgtccggcgc attgacccgg accggttccc aatcccgttc ccgttcccat tgacggatcg
      361 tcgaaacgtc gcggctgtga ttttcaatgc cgccggccgc ttcaatagcg gcaatgacag
      421 cgccttcgat aaaagggcaa tccaagagac ggattttccg atcgtccatg ctttcggcca
      481 ccatttcagc cgtcatgact gcgctgccca tatccatgat caaggctacc ccatcccctt
      541 gatctgccgc ttcaacggca gcactgattt tttcaaaact cgtaccgaga ctaccgtctt
      601 cgaggcctcc ggcagccttg accggcgcat ccggtgccat cataagggca agttccgccg
      661 ccccttcggc agctttctga ctatgtgaaa ccaatacgac tcctaccatt ttcttcaact
      721 cctatatctt atgccatttc tgccaagacc tgcagcaaat aaaaagccga tgtcgccccc
      781 gggtcttgat ggcctacact gcgttcgcct aaatagctgg cccgcccttt cgtagcaata
      841 atggtcttgg tatattctac ccctttttca gcagctgacg ccgcatcggc caaagcttcc
      901 tttaacgatt tgccggcagc gacatcgtcc tgcaaggctt ttaaggccgg gcacaaggca
      961 tcgagcatcg tcttttcgcc ttctgtcgcc ttgccgcgca tcttaatacc gtctacagca
     1021 gcctccatcg ctttgacaaa ggccgggcca tcgagttccg tcaagccctt gcaggccata
     1081 ccggctttca tgaaggccgt tccatagagc ggccccgaag cgccgccgac cgtcgacacg
     1141 agctgcatac ccacgccttt aagaagagaa ccgatatcac cgtcacgcaa agacggcagt
     1201 ttcttttcta cggctgcaaa gcctcgtgcc atattgattc catgatctcc atcgccaatt
     1261 tcattgtcga gctgggtcaa aaagtctttt tctgcttcaa tcttcttagc cattccttca
     1321 atgatggccg ccatctgctt cgtatccaac gaaatcatcc ccttttattt tttccaagcc
     1381 agtgtatctg ccggtgcgtc atagagggct ttcatgtcat cgtccaagcg caggagcgtc
     1441 aaggaaaatc ctgccatttc aatggaggtc atgtagttgc caaccatcgt gtcatagaca
     1501 tggacgccct gctctttgag atagtcctgc acaaaattat tgacgatata cagttccatg
     1561 agcggagtgc cgcccatgcc attgaccata acgacgacat ccctgccttt atagtcgagg
     1621 tcggataaaa tttcatcgat gtgcagcttg gctatttcat tagccgg
//
LOCUS       DAWDXZ010000423         1662 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12222_length_1662_cov_6.374611, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000423 DAWDXZ010000000
VERSION     DAWDXZ010000423.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12222_length_1662_cov_6.374611"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          436 a          398 c          349 g          479 t
ORIGIN      
        1 agaaaatatc acggcggccg cctcgttcga ccattcgttt ggcaatctta tagtgcttat
       61 tttcattgcg gtcacaggca agttccggtc gatgaaggtt gaactcaaaa tgagccttca
      121 cttcatagca aacgtcgctc aaatacttat aaatagacaa cgtattcatt ttcttattat
      181 agccctttgg ttttaccccg cgagactggg tacgaatctc cttcatgacc cgcatttcat
      241 cgatgtacca gatgatcgtc ggtttccaat aaataccttc aacgattcct ttcaaagcct
      301 ggtaggttgg cacagaatag gatgattttt cgccgcctat acgggtaatg ggatcggaaa
      361 ataacgccat tcttccgtat actttaaagg ttatttcatt tcgcaaaata tcccctcctt
      421 aataattaca aaatggattt gcttgttctt ctaatttgag accgatggcc gaatcataat
      481 acttttcccg caaggccaat ggtccttgca gagtctggaa aatagcaccc attcgctgaa
      541 gctgttccag ttcataggaa aagacattga ccgtatatgg ctgaaccttc ttgaacagtt
      601 tcaagagttc cttcggattt tcaacggaac catttaaggc cttaatgaaa tctttcccct
      661 cctcataagg aacgatgacg ccgatggttt ccgagtcgat gaccgaaaag gcattgcctg
      721 catcccaaaa agcatgacga taggaaaggt acttgcttat ttctatcttg tctcgttccc
      781 cacaggcctg atatccgata tcattcgctg aaataagttc taacatggta tacttcctcg
      841 gcggcaactc ataagatttc ttgagatctc ctcgatcgtt gtaatacttc ataaagtagg
      901 catccaaatt atctggtttt agaatgtcct ggatatcgat attatgaagc aaatccttgg
      961 ccattttgat gccgatttgg atgtcgggca gtttatcgag attttccctt tccatctgaa
     1021 agatcttgac taggcctgat tccatttcac catgacggtt acagcggcct gccgcctggg
     1081 ccagagaatc aaggccggcc agagcacggt atatcgtcgt gaaggacacg tcgacaccgg
     1141 cttcaatcag ctgcgtcgag atacagatga gcctgcgctt cgatgttccg ctatgaaagg
     1201 ccgccaaggc ctccttcatg cgtttaattt tatccattcg atgggccgga cacattttgg
     1261 tgctcaagtg aatcacctcg actttgtctt cgctgttggc agccagattc tttacagccg
     1321 tatagatgtc gcgagcctgg cgagtcaaat tgacgatgca gaggacactg cccgattcca
     1381 ccgcatcttt cagcagcaag ggtgccagcg tttcagccgt atgcaaggta tctttcaaat
     1441 actcgaattt tactcgctga aaagcctcat aaacagcctt tagatcatcc attatttccc
     1501 catcagactc aatgcaatgc atcttcatgt tatctaatcg gggctgcgtc gccgtgcaaa
     1561 gaacggccgt gacgccggca aagtgcttta agaaattcat agccagatta aacatatagg
     1621 tgcatttaac gggtaaggtc tgaatttcat cgaatatgat ga
//
LOCUS       DAWDXZ010000424         1656 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12271_length_1656_cov_10.337289, whole genome shotgun
            sequence.
ACCESSION   DAWDXZ010000424 DAWDXZ010000000
VERSION     DAWDXZ010000424.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12271_length_1656_cov_10.337289"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          348 a          381 c          486 g          441 t
ORIGIN      
        1 ggcctttttt tgcggatcct gaataatttc atcgtaggtg aagccggagt agacccagac
       61 ggatttgtcg ggcaagaccg ctttgatttc ctgaatcaaa ggcgtcaggc cgtcggtatt
      121 ttccatcggt tccccaccga ggagggtcaa gccgctgtta gtcggcgcct gaagccagga
      181 caggagctgt ttcttttctt tgtcggtcca ctcttggcca aacttagcat tttggtattc
      241 cgggttgaaa caattgaagc agtttcgcga gcagcccgtc acgaagaggg tcgagcgaat
      301 gccgggcccg ttagcgatat catattgtct catctgtgca taatgcaacg aaagtcacct
      361 cttatatgtg gaggacacgg ttgttgattt cctgtgtccg cccttcattc cagaaatttt
      421 cgcccaagta gccgcaggtc cggcggataa ccgtcagttt gttgcggtcc tgattatggc
      481 agcgcgggca ttcccattgg ttgtcgtcgt taaggatgat ttcaccgtca aagccgcatt
      541 cgtggcagta gtcgagcttg gtattgaatt cgccgtaggc aatatggtcg taaatatact
      601 ggatcatggt aagaacagcc gggatattat gggccatatt ggggatttcg gcgtagctga
      661 tgcagccgcc cgtcgatttg tcttcaaaat cggcttcgaa ggagaattta tcgaagacgt
      721 tgatcggttc gcggacgtcg acgtgatagc tgttggtgta gtaatctttg tcggtaacgt
      781 tggcaatgga gccgaaacga gccttatcga tcttgcagaa acggttggtc aggttttcag
      841 ccggcgtacc gtagagggca aagccgatgt tcgtttcctt cttccacttt tggatatggg
      901 cgttgaggtc gtccatgatg cgcatggcaa acttgcggcc ttccggcgtc gtgtgcgagc
      961 agtgcttggt caacatcgtc gcttcgtaca tgccgatgta gcctaaggac agggttgcgt
     1021 agccgccgta aatgaatttt tcgatcgttt caccgggctg gaggcgggca atggcaccgt
     1081 actgccagtg gatcggcgat acgtcagacg tcaccttctt caagagattg acgcgaagga
     1141 ggagggcttt tttgcacagt tccaagcgct tgtcgaggat ctggaagaat ttttcttcgt
     1201 cgccatcggc gagaatcccg atttgaggca ggttcaagga gacgacaccc atgttgaagc
     1261 ggccgtcgaa tttatagttg ccttcggcgt ctttccaggg gcttaagaag gaccggcagc
     1321 ccatgggact gaaaacgttg ccttcatagg attcgcgcat cttcttggcc gaaatgtagt
     1381 cgggatacat ccgttttgcc gtgcattcgg cagccaattg ggtcaggtag aaataggggc
     1441 tgccttcgct gacgttgtgt tcgtcgagga cgtagatcag tttcgggaag gccggcgtga
     1501 cgtagacgtt ggcgtcgttc ttaatgccct gaatgcgctg acgcaggagt tcttcatcga
     1561 tgagggccgc ttccttagcg tattcgtaat cgggcttaaa gtacatgaac aaggtcacaa
     1621 acggagcctg gccgttagtc gtcaggaggg tattga
//
LOCUS       DAWDXZ010000425         1655 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12280_length_1655_cov_4.283750, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000425 DAWDXZ010000000
VERSION     DAWDXZ010000425.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12280_length_1655_cov_4.283750"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    925..993
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          465 a          332 c          291 g          498 t
ORIGIN      
        1 tcatctcgaa taatgacacg cattcctggt gcaaattcca acatcttcat agcctcccgc
       61 atttcctttt ctagtcttgc ttaaagttat gcaatataat aatattcgac atagaaggct
      121 cgatttcctg ctcatttgca acaaagaata tggcaccaag acaaaagagc tgtgcttaga
      181 attgcttcta ggcacagctc tttttatgtg ctacttccat cttttgacaa tttatttatc
      241 atcgagcgat tgtttcagtt ccagagtgct gccatcttgg aattttactt ttgggatttg
      301 cagtttaagc tgatacccat taaggccatg cttaacaaaa gtgcattata tattgtaaaa
      361 ttagacacaa aagagaaaat ttctgctaac aaaaacaggg cttcaataaa ttgaagccct
      421 accagtctta gtatattgca ctcatgatct tcagtagatt ttgatacgtg tcgacttgtt
      481 tataaatacc ttgtcaacgg aattcaatct tcctgctttc tgcggtatga ttgaaacaga
      541 agagattccc tctgacggag aaatcgtacc gtcattagta ggtacgaaac tatagaaaaa
      601 gattccccca tggctggaat gccgtcatta ttattatcgg tttgcaccgg atatacaggg
      661 tgctttttat cttcctgccg tgctgcataa cttcactcca ctcactttcg tggtacaata
      721 aaattattat acagtatttt caaaggagta tgacggtatt atgaagaaac gcttaatttt
      781 aaaaggtcta atatgtctag caaccgtgct ttttgcgttc aacttaaata cgactcaagc
      841 ccaaacagac gacgccaggg ttctcggcgg tatcagtaga tctacggata ttttcaccta
      901 tctgaatacg tatccgtaca aactnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtcagat catcgatttg ctcccgacat
     1021 ttataacgac ctctatctgt ctgaagataa atgtgttgcc gttgatttat ggaacgatag
     1081 cttaggccaa atttttcttc tcggccccgg ctataaaacc gataaaggta tcgaagtcgg
     1141 catgacgatg aacgatgtcg aatacgctta cggtcccatt tatgattcca gtaaagcccc
     1201 tcgtgactac acgcagcgct acggcatcta cacagattac ggaaacgatg gctactataa
     1261 aaagtatcgt gcttacggcg ttattgaata cgtgtctcca aataatgaag gcttgaattt
     1321 tgtaatcgat aagaagacca aaaagatcgt cctgattatg taccaagcaa atcatcacgg
     1381 aaatggaacg gccatgtctt atgtcaaact atacaactta cttcctgaaa gaaattaaac
     1441 gccttttgcc gcatctcagt gagtcacgat gcggcttttt gcgcttctat gctgctaacg
     1501 gtataaatca gtcctacttg tcgcacttgg ccggcctcat ctaatgctga taaccaaaaa
     1561 tttgagttaa tacctgaatg gatatttcat atgttgtgtt tttaattcgt cgtagtagtt
     1621 tctttctctc gggagtaaat catttatatt attaa
//
LOCUS       DAWDXZ010000426         1655 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12276_length_1655_cov_9.084375, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000426 DAWDXZ010000000
VERSION     DAWDXZ010000426.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12276_length_1655_cov_9.084375"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          391 a          414 c          456 g          394 t
ORIGIN      
        1 ttgaggtaag ccgtctgcca ggcaacgtaa gcgtaggctg ccgagtggga tttattgaag
       61 ccgtatccgg caaaatagac catgaggtcg aaaattttgt tggcgatttc atcagaaatg
      121 ttattcttcc gcgtcccttc gaggaagtta gcccgctggg ctaccagaat ggactctttc
      181 ttcttgccca tggcccgacg cagcaagtca gcctgaccaa gagagaagcc gcctaaggcc
      241 gaggcgatct gcatgacctg ttcctgatag aggatgacgc cgtacgtatc ctttaagatc
      301 ggttccagct tcgggtgcag gtaggtaacc ttcttctgac cgtgactgcc cttgatgaag
      361 tcatcgacca tgccgctgcc caaggggccc gggcgatata gggccacgag cggaatcatg
      421 tcttcaaaat gcttcggcgc cagttcctta accaggttgg taatcccaga cgattccatc
      481 tgaaagacgc cggccgtatc gccttcggtc aaaagcttac acgttttcgg atcgtcgaga
      541 ggaatagcat cgatgtcgaa atcgatgccg tagttcttat tgatcatctt gaccgtatca
      601 ccgatgacag tcaaggttct gaggccgaga aagtccatct tgagcagtcc caggtcttcg
      661 acgagatcct tttcatactg cgtcgtaagg tcgccttcct tggagtgttg gaccggcacg
      721 taatcgtcga cgggcgccgc cgaaatgacg acgccggcag catgggtcga cgagtggcgc
      781 accaagcctt cgagagattt tccgaaatcg accaaacggt ggaccgtatc gttagactcg
      841 tattcactgc ggaactcttt gctcatctgc agggccttgt cgagggtcat gcccaattca
      901 ttgggcacca gttttacgat gcggttcact tcacttaagg gaatgtcgag gacgcggccg
      961 acgtcacgca aaacggcttt ggccgccaag gtcccgaagg tgatgatctg cgagacgtgg
     1021 tcgatgccgt attttttcgt aacgtactga atgactcgac ctctgttttc ataacagaag
     1081 tccatatcga tatccggcat ggtcactcgt tcgggattta agaatcgttc aaagaggaga
     1141 ttgtacttca aaggatcgag gccggtaatg cccaagaggt aggcaacgac agaaccggct
     1201 gccgaaccac ggccggggcc gacgggaatc tcatggtccc gagcatattt gacgtagtcc
     1261 cagacgatga ggaagtatga cggaaacccc atggtatcga tgacgccgag ttcataatca
     1321 aggcgttctt ttacttccgg cgtaatggga tcgtactttt cagaaagggc ttgttcgcag
     1381 agctttcgca gataggaaga atccgtttcg ccgtcgggaa cgggaaactg cggcaagtgg
     1441 cggtcgtcaa aggagatatc gacgttgcat ttatcggcaa tttccaaagt cgtatccaag
     1501 gcttcgggca tatccgaaaa gaggtcggcc atttcatcgc cgctcttcat ataaaattca
     1561 ttattataaa atcggaaatg ggacggatcg tcgagggtgc tgcccgtcga gatgcagagc
     1621 ttgatttcct gagccggcgc atcggcctga tcgat
//
LOCUS       DAWDXZ010000427         1644 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12352_length_1644_cov_8.067338, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000427 DAWDXZ010000000
VERSION     DAWDXZ010000427.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12352_length_1644_cov_8.067338"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          371 a          383 c          519 g          371 t
ORIGIN      
        1 gtctacgcct tcgagatcag gaaggaggag gcctgtacgg gcatggctca tgacgatgac
       61 gccgtacttt ttgggattga gctcgctggc tgaggtgacg gcctccggcc tccctaagac
      121 gtcgacggaa atatcgatat ctgcgagctc ttctaagctg actggtgaga aacgcggatc
      181 ccgtgtggcc gctgagacgg cattatggat gatttcactg gccgtattca gatgcatggg
      241 aaggaaggta ccgatgcagc cgcgcagacg acttccttta tggagagata cgaaggtacc
      301 ggcctgatcc tgaagttctt ccggcaggtc tttggggacg gccattaagg catgatgctg
      361 aaggtagtag gtgatgcttt cacgggccaa gcggactcgt atgtccgagg gagcccgttt
      421 tacgacctgg tcttcctggc cttcgggaag gtagagggcg acaccgtagc cgacgccaaa
      481 gggggcttca taggcataga ccggcatgac cgggcggaag tgacgcaggg caccgaacaa
      541 aaagtagacg gaaggcaggc cgcaggtggc gatttcgtcg ataaaactgc ggctgaggtc
      601 ggtgagtcct gagagatttt gcttgagcag ggcttccatg accagcttgt cgaagacggc
      661 cccatttggc gtgtagccat tgggcgagcc ttgaagcagg cggtgggaca gatcgcctga
      721 ggcgatgacg gcaatgcgcc ggccaagggg tttggcggcc tttatgacga tgtcgcctaa
      781 gcgggtcata ctgtcgtaat cgtcaaatcg gggagccatg atgacgatag gtccggtgaa
      841 gccggcttta cgaaggtaat ataaggggac gaaggtgccc tggtcgacgc tgagaggatg
      901 gccgaagcga tcgctccagg cggcgtcgag gcgatagata tcggtctgtt gactggcctt
      961 gttgacaatt tcttcggcaa ggtccatatc ggtccgaaca tccatcgcaa gttccggata
     1021 gccgaaggcg gccaggttgc cgtagaggcg aggcgcggcg atgatagcgg ctccgtcggg
     1081 aaatacgtaa ttgtggggcg ataagatgac caatgtttcc ggtttttgac tgacaatcag
     1141 tgccgaagcc gcttcgacag cctcgacggt ggcggtcatt tttgacaatt cagggccgcc
     1201 tatttcgggc agcatgatag gcggatgggg aaagaatccg gctgctgcac agacgtgttc
     1261 catcgtgatt acctcctttg ctgggatgga taattagagt caactttatt ataccccaat
     1321 cggccggggc ctcatagccc gggaccgatt ccaggcatga aaaaaaggac tatctgatga
     1381 tagtcctttt ggtcaatatt cagtatcttc taaaaggctc gtctgtccaa agcgggtccg
     1441 aagatccttt cgttccatct tccatttgga agatgcaatg gacatggtcg gatcgtaatt
     1501 aggggactgc atgttgagga tgccgcagat gaattcgtat tccaggtcat tggtaaagaa
     1561 cttatcctta ttggccttga cggccgagac gacttcagga ttgcgctcct tgtaaccgtc
     1621 ggacagatag acgaacatgg ggat
//
LOCUS       DAWDXZ010000428         1643 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12362_length_1643_cov_6.295340, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000428 DAWDXZ010000000
VERSION     DAWDXZ010000428.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12362_length_1643_cov_6.295340"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1370..1379
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          403 a          504 c          409 g          317 t
ORIGIN      
        1 tgcccatcgt cgaaggggct acggtcaata ccaaatacgg tccggtcaag accgaccata
       61 tcctctttat cgctgccggt gccttccacg tatcgaagcc gagcgacctc attcccgaac
      121 tccagggccg tttcccgatc cgcgtcgaac tccagtcgct gaccgtcgaa gacttccgcc
      181 gcatcctgac cgaaccgagc cagtccttgg tcaaacagta tacggccctc ctcgaaacgg
      241 aaggcctgac cctggaattt accgacgacg gcatcaatgc cattgccgaa tacgcctacc
      301 gcgtcaaccg cgaaacggaa aacatcggcg cccgccggct gcacacgatt ttggaaaaga
      361 tcctcgaaga tgtagcctat gaagcgcccg atatcgatga aaaacacatc gtcatcaacc
      421 aagaattcgt tgccggcaaa ctccacgacg tcgtccagaa cgtcgacctc agccattaca
      481 ttttataaag gacgcgactg atgagacctt acgcaaaatt atatctgacc aaaaaagggg
      541 aacgggctgc caaaacgggc catccctggg tctatggtga agaagtgacc cgcgtcgacg
      601 ggacctataa gacgggcgac atcgtcgacg tctacagcga caaagaccgc tacctcggca
      661 cgggctttgc caacgatatc tctaaaatcc gcgtgcgcat cgtatcgcgc aatgccaacg
      721 atcgcttcga cgacgctttt tggcagcgcc gggtaaaata cgcccttgac tatcgccgga
      781 cggtcatggg cgaaacggac tttgcctgct gccgcctcat tttcggcgat gccgatgaaa
      841 tgccgggcct taccgttgac cgctactccg acgtcctcgt ggcccagacc ttatgctacg
      901 gcatggacca ggtgaagccc atcatcttca aggccctggt cgatgaattg aaggccatgg
      961 gcgtcaccat ccgcggcatt tacgaacgaa acgacgtcaa agtccgcgaa ctggacggca
     1021 tggagcagta caaaggctgg tacgaagccg attacctgcc tcagccggga agcgtcataa
     1081 cgaccatcga agaaaatggc attcgctacg acatcgacgt cgaaaacggt cagaagacgg
     1141 ggttcttcct ggaccagaaa tacaaccgtc tggccgtggc taaaatcgct gccggcaagc
     1201 acgtcctcga ctgctttacc cataccgggg cctttgccct caatgccgcc aagggaggcg
     1261 ctgccagcgt caccgccgtc gacgtgtctc aagaagccgt cgacatgacg acgaccaata
     1321 ttcatcgcaa cggcttagaa tcgatcgtaa cggccaaaca ggccaacgtn nnnnnnnnnc
     1381 tatttagcta cctgctcgtg ctcccacttc atgcgcgacg acctcttccg ccagatgctc
     1441 cacgatgccg cgaaagatgc cggcgttcac cttcgtcaaa tcgaaggccg ccagcagtcg
     1501 ccggaccatc ccattctttg gaacgtcccg gaaacgaatt acttaaaatt ctacctattc
     1561 caagtcgtat aaaggggaac gccgctgttt cagtgctgat gctgagacgg cggcgttttt
     1621 gcataaccgg ccttgtagcc aag
//
LOCUS       DAWDXZ010000429         1642 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12372_length_1642_cov_5.457467, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000429 DAWDXZ010000000
VERSION     DAWDXZ010000429.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12372_length_1642_cov_5.457467"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          453 a          432 c          429 g          328 t
ORIGIN      
        1 ggattcatca agcttcgcga tgggcaaaac cggctacgat atgcttacgg cacatattat
       61 agacgacggc caaaacgatg accgtaaaca gccagctgac cggataggtc gccatgagga
      121 ccgaaaagtc aggacgagcg gcaaaggccg tgtaaagcca gacgattcgg acgccgcaga
      181 ttccgatgag ggccacgatg gccggcggca gagaataacc gtagccgcga agggagccgg
      241 acaggatgtc gaccatgccg ttcaggtaaa acgtaccgag aacgaagaac aatctcgtcg
      301 tccccagggc gacgacagcc gcatcgtcgt tgaacaaacc gacgagttcc ggtgaaaaga
      361 agaggatgag ggctgtaacg acaaagagga aggccgcttc aacgccaaag gtaactttcg
      421 taatgcggaa gcagcggcgc aggttgcggg ccccgtagtt ctggccgata aaggtcgtcg
      481 tcgcctgact aaagccattg acgaagcagt acgtattggc ttcaatgata aagcctgccg
      541 ccgaagcagc catagcttcc gttcccaggc tgttgatggc cgactggatg agtacgttgg
      601 acaaggcaaa aatcatgccc tggatcccgg ccggcagacc gatgcgaata atgcggctca
      661 attcatcttt agaaaaatga aggtcccgca aattgacgcg aatccgatcc gtcgtccgac
      721 agagcaatac caagagaatc agcgaatttg cggcataggc aatgaccgtc gcccaggcga
      781 taccgacgac accgaaatca aagaccgcaa ccgacaggaa gcccaggata atattgatgc
      841 cgttggcaat gaccaagctg taaaggggcg tcttactgtc gccgcagctg cggaaaatag
      901 ccgcttcaaa attatacagc gatagaaaag gcaagcctaa taaaaagatg cgcaaatagc
      961 tttcggccat atcatagacg tcaaggggca cttccaaaat gaggaaagcc ggatagacga
     1021 tgaattcgcc gatgagggtc aataatacgc ccagtgctac cgacaaaatg atggccgtct
     1081 gtaccgtctg ggacactttt tcgtcgtgtt tagccccgag atactgggcg atggtcacgt
     1141 tagctcccag ggaaatcccc atgagcagca tgaccatcat gccgatgacg ggcatattgt
     1201 tgccgacagc cgccatggcg cttttcccga caaaatgacc taataaaaag acgtcagcac
     1261 tgttaaagag ctgttcaaat aatcccatga gtccgatcgg catggcaaac agaaagagtt
     1321 tgtcggcaat cgatccgttc agcatgtcga tgccgcgccg ctggttctct cgtttatttt
     1381 ccatatatac accttattcc ttcattatat attcatgaca gagtccaagg ccagacggcg
     1441 ttaacgccgc ccccttgtcc gcaatatttt taatatccta attcactcag ccaggacagg
     1501 actttatcct tagcactgcc gacgtcaccg gctgcagtgc cgtacgcctt cgtttcgaca
     1561 tcggctccct tttcgatgga ctggacgtgt tcgaccgtat cgcccaatcc gctgctgcca
     1621 taggttccga agaggatgat ct
//
LOCUS       DAWDXZ010000430         1635 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12421_length_1635_cov_5.079114, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000430 DAWDXZ010000000
VERSION     DAWDXZ010000430.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12421_length_1635_cov_5.079114"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    598..607
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          243 a          430 c          461 g          491 t
ORIGIN      
        1 gacgaacgtt tcttttccat tcgtttttca gcaacggaac gagccgtttt ttcgtcggtt
       61 acgttcaaga tgtcgccggc ttccggtacg tcattgaggc cgaggatttc gacaggcagg
      121 gacggttcag ctttcttgac ccgttcgccc cgttcgttgt tcatggcacg aactttcccg
      181 aaggccgtac cgacgatgat cgtatcgccg acgcgaagag tccctttctg gatgaggacg
      241 gatgcgacag gaccgcggcc cttatcgagt ttggcttcga tgacgacgcc ttgggccgga
      301 cggttcgggt tcgctttaag atcctgtact tctgccaaga cgaggatgtt ttcgaggagt
      361 tcgtcgagac cgattttctt atgggccgaa acgggaacca tgatggtatc gccgccccag
      421 tcttcactga tgaggccgta ttcggtcagc tgctgcatga cgtgttccgg attggcactt
      481 tctttatcga tcttattgat ggcgacgatg accggtacgc ctgccgattt ggcatggttg
      541 atggattcaa tcgtctgagg catgacgccg tcgtctgcag cgacgacgag gacggcannn
      601 nnnnnnntga gcgccgcggg cacgcatagc cgtaaaggct tcgtggcccg gcgtatccat
      661 gaaggtgatc ttcttgcctt cgtaacggac ctggtaagcc ccgatgtgct gggtaatgcc
      721 gccggcttcg tggctggtga cattggtctg acggatggcg tcgagaaggc tcgttttacc
      781 gtggtcaacg tgacccatga tggtaacgac aggcggacgg gttttcaggg tttccggggc
      841 atcgatgatt tcttcgattt ccgtcggatc ttccggcgga gcttccatgg taacggtgac
      901 gccgaattca tcggcaatca aagaggccgt atcaaagtcg agttcctggt tgattgtcgc
      961 catgatacct cccatgagga gcttcttcat gacttcaccg acatcgcggc ccagccgttc
     1021 tgcgaaatct ttaacgatga tcgtttccgg cagttcaacc gacgtcggat agttcttctt
     1081 cggcatttca gccgcgacag gagcctgatt tttctgattc cgcttgtttt tatgcttgcc
     1141 cttgtttaat acgttggaca agagcgtatg cggacggctg ccgccgttat tgcggttgtt
     1201 gcggttctgc tggccgccgc ggcgattgtt gttcttattg ttgccgccgc tgcggttagc
     1261 gccttcgttt ccgccctgct gggagttgcc gaagtagcgg gtacgtttcg tatcgccgcc
     1321 ctgacggttc tgctggccgc ctttattgcc gctctgatgc tggcgggaag caccctgctt
     1381 ttgaccggcc tgggcatttt tagcgttatg gccctgttga ccttgctgcc cctgttggcg
     1441 tttggcattc tggttctggg tattctgcgt tccctgattg ttctgtctgc gcgggttgct
     1501 ctgttttctg tttttattct gtcggctcgt ttcgttcact gttcggctat ttccttcccc
     1561 tgtcgtttta ttttcctgtt tcttttccgt ttttctttcc ggtctgcttt ccggcttcct
     1621 ttccggcttc ctttc
//
LOCUS       DAWDXZ010000431         1634 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12428_length_1634_cov_8.620646, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000431 DAWDXZ010000000
VERSION     DAWDXZ010000431.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12428_length_1634_cov_8.620646"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          475 a          401 c          422 g          336 t
ORIGIN      
        1 aacatcttat gaagccataa aaccagcctt ttcaggactg tcggaaaggc cataaaggat
       61 ccgctgttcc cgtatgacga agaaaatgga aatgataagg gccaaggcgt cactgatggg
      121 ctgggccatg aagataccat tgatgtccca cagtacaggc agcatataga tgagcgggat
      181 gaggaacaga atctgccgta aaaaggtgat gacgatggaa ggcatcggtt tggcaatgga
      241 ctggaagaat gtcgtcacca gcatgacaaa gcccagaatc ggggtgatgc agaaattggc
      301 ccgcagtccg ggaacgccga tggccagcat atcttccgta gccccaaaag ccgccagaat
      361 ggcctcggga aataccatga tcagaatcca gatgatgcag gtaatgccga ccgaaaaagc
      421 ggaggcctga tataaggtgg ccatgacccg atggtactgt ttcgctccga agttgttgcc
      481 gacgatgggc tgaatcccct ggctgatacc gctggccggc atgatgacaa aggtcatcat
      541 actagagacg acggcgtaga cactgatggc cgtatcgccg ccgtattggc ctaattggct
      601 gttatagacg aggctgatga gacccatggc catttgggca atccaaaagg caaagccgca
      661 ggaaatgata gtccgcgaca gccaggcatc gaaggcaaag gtatcccgtt tgatttcgac
      721 cttcgtatgg cctttccaaa cgaggtaagc cccaaaaaag gccgaaagga attgaccgat
      781 aaccgtcgcc caagctgcac cggtgacgcc ccagtcgagg ccggccacaa agacggcgtc
      841 gaggaggatg ttggtcacgg ccccgacagc cgttacgccc atccccagga cgggctttcc
      901 ggaaacgcga acaaagtcgc aaaaaacctg gctcagccct tgaaagaggc agcccagggc
      961 cacgatgcgg taataagcga gggcgtattc ataagccgtt tcggtaacgc cgaacacctt
     1021 taggatagga tcggcaaaca gcagcaaaaa gacggacagc agcacttcaa agacgatgac
     1081 taaaatcagg gaattgccaa aggaacggtt catattctcc tgttcccctt ttccggaaaa
     1141 caggctgaac agagacgatc cgccggcgct gcaagtcagg ccgaaagcca tcatcatgta
     1201 aaaaagagga aagcaaatcg aaagaccggc caaggccgac gtgccgttga agttgcctac
     1261 aaagatccgg tcgacgatgt tataaatggc tgtaatcaaa agagaaatag ccgccggaat
     1321 ggagtatttt aaaatcatgg gaaagagctt tcccgtcgaa aaatcatttt tttcattcat
     1381 attaaagtgc ctcgcctttc tatcatatct tagtttccta agtatttagt taaagagaaa
     1441 ggacaacgcg tctgcccttt cagtcagaca ggttatccat catcttctca acgaccttca
     1501 caaagacttg caggtccttt ttagaaatgt ccttgatcag tctttcatct aaggccgtca
     1561 aatcgtctat gacttgctgc cgataccgca aggccctttc cgttaagaca atctttttta
     1621 agcggttgtc atgg
//
LOCUS       DAWDXZ010000432         1620 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12544_length_1620_cov_6.828115, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000432 DAWDXZ010000000
VERSION     DAWDXZ010000432.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12544_length_1620_cov_6.828115"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          385 a          398 c          474 g          363 t
ORIGIN      
        1 tgtccggtct gtaccttccc gaactgtggt tttggaaagt aggtgttctt gtgaacccgt
       61 ttacacatat tgacaagaat ggcgatgccg tcatggtcga tgtcagtgga aagacggtga
      121 cgtcgcggac ggccatagcc tgcggccgta tttcgatgag ccgtgagtgt tatgatgccg
      181 tgaaagccgg cgctatgaaa aaaggcgacg tcctcggcgt cgcccgcgtt gccgggatta
      241 tgggcgccaa gcggacgagt gaactcattc cgctctgcca tatcttaaac cttacaaaat
      301 gcggcatcga atttattttt catgacgatg tctgtgaaat cgaaggccgc tgcaccgtta
      361 agtgcgacgg caagaccggc gtcgaaatgg aggccctgac cggtgtgtcg acggccttac
      421 tcacggttta cgacatgtgt aaggctgtcg ataaaaaaat gtgcctgaaa gacattcatc
      481 tcgtcgaaaa aataggtgga aaaagcggcc atttccaata cgatggaggc tctgaccatg
      541 aaggatagat gggggcggac cattgattat ctccgcctgt cgctgacgga ccgctgcaac
      601 cttcgctgcc gatactgcat gccggatgac gtcgaaagcg tcggccatga tgcggtccta
      661 cgctatgagg aaatgatgat tgtctgtaaa gctgcgctga agctgggcat cgaccgcttt
      721 aagatcaccg gcggcgagcc cctggttcgg cgaggtgccg tcgatttcat acgagccttg
      781 aaaggcctcg acggtgtccg ctcggtaacg atgaccacca atgggatgct gctggaaaaa
      841 gatttgccgg cgttgtccga ttttggcatt gacgggatca acatcagttt ggatacctta
      901 gatgccgagc agtacagacg gattaccggc ggcggagact tgtccgtcgt ccttaaggca
      961 ctccatgccg ccgtagaagc aggtgtaaac gtcaaagtaa acgccgtatt gctggctgaa
     1021 acgagaaatc aaatcgtaaa attggcagaa ctcgcgcagt cgctggccgt cgacgtccgg
     1081 tttatcgagc tcatgcccat tggttttggg acgaccctgg ccggactcac tcgcgatgat
     1141 tgcctgaaag tcttgcgccg ttcctatccg gaccttgcgg cgaccgatga agtgcgcggg
     1201 aacggcccgg cagactatta taagagccgg tatctttacg ggcgaatcgg ctttattgcc
     1261 gccaacagcc acgctttctg cgcgtcctgc aatcgcttgc gcctgaccag tacgggcttt
     1321 ttaaaaccct gtctctgtta tgatgacggc gtcgatttac ggaacatcat ccgcagcgga
     1381 cgccctcaga ttgagatgca gcaggccttg acggaagcca tcgagagggc cgtttacgaa
     1441 aagccggcga aacactgttt tggtgagaaa gcggccatta gcgagcataa aatgatgagt
     1501 cagatcggag gataagaaaa tgggaaaaat acaggccatt tgtagcagcg acgttcgggg
     1561 aatcgccaaa agcgtcatcc ccgaagggcg ttttaaggtc gattgggggt tagaaggcga
//
LOCUS       DAWDXZ010000433         1613 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12610_length_1613_cov_6.113607, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000433 DAWDXZ010000000
VERSION     DAWDXZ010000433.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12610_length_1613_cov_6.113607"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          330 a          435 c          475 g          373 t
ORIGIN      
        1 taaaagataa gatgacggcg ccgacgaggt tgcccaggta gcagacgacc cacaagccaa
       61 tgacgtcggt caaggtgacc gtcttttgcc gccagccatt gtagaggacc atggcgttgc
      121 ccgtaaagag ttcggcaccg gcgatgatga ccaggctgag ggcgatgcca aaggacagac
      181 ccatggcgat tttattcatc ggaatcggtc ccaaagtccc ccctaccgta aaggacagga
      241 ggatgccgaa gccgatatag gcaccggcca gggccgacat aagaaagtat cctaagggat
      301 tctttttcaa taagcggact ttggcggctg caccggccgc cgtcgccgca tattcgtcat
      361 gatacatcgt agtctgcccc ctacttcaat cccgagtagc ggatgccgtc ccagtacagc
      421 ggtttgtaga tttcggcctt cggcggcaga ttgacgtcct tcatgatcca gcgcaggaat
      481 tcttccgttc ccgtccggtc gacgatgtag ccgatgtgtt ccttgccgcc cggtgcttca
      541 ggatcgatgt atttctgagc gtaggcgtag acgttgctga tgatcttgac gacactgtca
      601 gcgtcgagcc atttgatgaa gtcgatgccc atgcgggggt tcttcttgcc gctgcggccc
      661 aggagagtca tgcggaaata ctgggtcttg ctccgcgtcc aggcacgggt cgggcaggcg
      721 tgggcgcaga cgccgcagcc gatgcatttt tcgacgttgc gtacgggccg gttcttatcc
      781 atcttcaagg cttcgacgga aagctgtttg cacttcttga cgcaggctcc gcagctgatg
      841 cagcgcgtcg ggtcgaactg gggctcggtc atgccgatga tgccgaagtc atgcatgcgg
      901 accttgccgc agtcgttggg gcagcccgtg agagcgactt tgacgtgaag gtcattgggg
      961 aaaatagcct tttcaatccg ttcggccagg gccgtcgtgt cgtagttggc aaagggacag
     1021 atgcggttgc cgacgcaggc catgatgtta cgcgtaccgg acgaatcgta gccgcggccc
     1081 ttttccgact ggttgatgcc caattcttcg atgatgggct gcagggcctt gttgacttca
     1141 tcgacctttt caaagggaat gcctaagatt tcaaagccct gacggatggt cagatggact
     1201 tctccgctgc cgtaagtttc ggcgatttcc tgaactttcc ctaagacgtc ggcccggaga
     1261 tggccgccgg gaacgcggac gcgggcggac gttacgccgc ggttcttggt gacgcgaaag
     1321 gcgtttttct ttaacttgcc acaattcata tcaatcatcg ttcttccctc ctagtcgata
     1381 aacgagcgaa ccgtcgtata tttgaatacc gggccgtcct ggcagacgta gagctcgccg
     1441 atgcggcagt gaccgcattt accgaggccg cagcacatct tgcgcgattc ggacaaccaa
     1501 attttttctt catcgaagcc aatttttaag agttcctgaa tggagaattt gatcatcatc
     1561 ggcggaccga cgacgacggc gatggcgttg tcgaagtcct tgagcggcag ctg
//
LOCUS       DAWDXZ010000434         1613 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12606_length_1613_cov_8.447368, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000434 DAWDXZ010000000
VERSION     DAWDXZ010000434.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12606_length_1613_cov_8.447368"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    520..529
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          331 a          469 c          461 g          342 t
ORIGIN      
        1 gggcgctgcc aaggacgggc gtcgtcattt tcaatcatca tgacggctaa ggcgtcgtcc
       61 cctctttccg taaagtcagc agccgtaccg cgaaggatga cttcgtcgtc ataagagaga
      121 ttacagccga tggcaacccg cacggctcca agccctctgt cacagagact gcggcagagg
      181 gcagacggtg attcgctgcc gccggtgagg acgaagacct tggcatgctg gcgtacagcc
      241 ggaataagcg gttcctgccg accatggcgg ctgacgatga agacgtcatt ccaagatgtc
      301 tccaaggcgg cggcaaagta gaccagactg ctgatgccgg cgatacggat cacggtgcag
      361 tccgggatgt tcttcaataa ggctgccaag ctgaaaaagc cgacatcccc cgatactaag
      421 acggccatag gcccatccgc atccgtccgc gacgccgcca ggcggcagat ttcatcgcgc
      481 cgataagtcc ggaccaattc tttccccgtc ccctcaaagn nnnnnnnnnc atccgcttgt
      541 cgccgacgag aagggtcgag gcttcgatgg cacgtttggc ttgtacggtc aaaagattcg
      601 ggtttcccgg accggtgccg ataacataga tatgcatata acgtcctttc cctgacgaaa
      661 taatctccct gtcagcgttc aatgatgatg accgtcgccc ccgttcgttc ggcccctttc
      721 agtttctctt ggaagccgcc ggtgtggcct gaatttttag tgacgacgta gcgggccccg
      781 gtctctttaa acatggccgc attgaggtcc gccgagaaag gcccctgcat gcagatcagg
      841 cgcgccggcg ggtagcccaa atccaaggcc cgttcgaggg aggccttgac cggtaaaata
      901 cgggcataga ctcgattttc atagcccggg atcttggcaa aggcatcgag gttcttactg
      961 cccgtcgtca aaaatacgtt cccttccgta tgactcagga cttcgacggc ttcgtcgacg
     1021 gtgcgaacgg caatggtgcg gccatccgtc cggtacgttc cggcccggcg gttgatgcgg
     1081 cgataaggga caccggcctt ttggcaggct gccttgatgt tgtcggtgac gaccgtcgca
     1141 tagggatggg tggcgtcgat ggcaaggcgt atccctttgt ccttcaaaaa tccttccatc
     1201 tgtccctcat ccattcggcc ttccaggacg gtcacataag gacttttcgg caataaggac
     1261 gcgccgtagg ccgtcgctac ggagacgaag gctttgacct gaaagcgtgc ggcgtaaccg
     1321 gccagtttcc gcccttccgt cgtcccgccg gcaatccaaa cggggccaat catagtcgat
     1381 agcctcgagg cgtgactaac ttgccgttta agaccttagt ctggctgttg ccgataaaga
     1441 ccgtgcagaa catatcaagg tgctgcgtcc gcagttcccc caaggtgaga atcttgaccg
     1501 cttggccatc gcggccgata ttgcggacga tgcctgccgg cgtatgggcc gactgatggc
     1561 gcaggatgat gtcacaagcc ttttcgaggt agtcagaccg cttcttgctg gcc
//
LOCUS       DAWDXZ010000435         1611 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12626_length_1611_cov_6.703728, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000435 DAWDXZ010000000
VERSION     DAWDXZ010000435.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12626_length_1611_cov_6.703728"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          507 a          380 c          375 g          349 t
ORIGIN      
        1 tcatagcttt caacgcggcc gtcactgccg ggaatgtact gaataccggc cttttttgcc
       61 tgaatacggg cattgatctt atcgccgaac atgatgaggt gttctacttt aggaccgata
      121 aagataatac cttcttcttc acagcgtttc gctaagtccg cattttcgga caagaatccg
      181 taaccggggt ggatggcatc gacgtcgtgc tcatgggcaa tacggataat atcttcaata
      241 tctaaatatg catctaccgg ttttttgcct tcaccgacga gatacgattc atctgcacgg
      301 aaacgatgca gcgacagcga atcttctttg gaataaatcg caatcgtgcg gattcccagt
      361 tcattacaag cccggaaaac acgaatggca atttctccgc ggttggcaac taaaaccgaa
      421 cgaatttttt tcatgaaaat accctcctgc gtataaaaat tgaatcagac cggtaaacgc
      481 acagccaatc gacgcccata cattcaccgc tttctctatt atagcaaaat ggaaacacat
      541 tacaagggac ggccactttg tatacaaaaa aatactgtat acatgaatcc taaaaaaaca
      601 cgcaaacaag actatctacg tggatttgtg gttttcatgg gaattgagtc tactcaacta
      661 cccgccattc atctcccgcc taagaggaag gagtcttctg gtgggttaag ataaaatcat
      721 ttatttcaat aaatatacct agggcttcat aaatctcata accacaatcg ttccctcata
      781 gagaaagatc aagggcacgg caatggccgt ctgggtaatc atatccgtcg tcggcgatac
      841 gacaccggca aagatgaaag cccctacgat aaagaaacgc cgcttctgcc gcagggatct
      901 cccggtcacc aagcccaaac gcgccaggat aatcagcgcc agtggaagtt caaagccgat
      961 agcaaacggc aggacgaagg cgatgaaaaa agacaaatag gccgctaaag aaaacatagg
     1021 ctgtagagag agggcagaaa aactcagaaa aaattggacg gcttggggaa agacgacgaa
     1081 gaaggaaaaa gcagccccgc cataccacaa aacaagagca gccggcataa accagacgaa
     1141 ggccttggcc tcggtatcgc gaagtcccgg ccttaaaaac cgccatagtt cataaaagtg
     1201 gatgggcaag gtcacaaata agcccgtaac ggcggcgatt tgcaggtaag aaaaaaagac
     1261 ttccgccgga ttcagaaaat aaagctttcc ggccggcgcc gagatgacgg caatgatatc
     1321 ttcgacatag gtataggcga cgatcgtacc tatgagccag gctgccagcg aaaagaggag
     1381 gcgccgccta agttcagcca gatggaaggt gataggtgct ttttcttcaa tcatagaaat
     1441 cccttcttta acgataaata gaaaaggccc tgccaagtag cgacaggacc ctttcacggc
     1501 cgtacaagaa ttatacgtaa tgaatgcggg atggatcata gcggtcttca tgcggtttgt
     1561 cttctgctgt tgcctgagga taaccgagag caacgatagc aaagggggta a
//
LOCUS       DAWDXZ010000436         1609 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12646_length_1609_cov_4.259331, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000436 DAWDXZ010000000
VERSION     DAWDXZ010000436.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12646_length_1609_cov_4.259331"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          426 a          430 c          357 g          396 t
ORIGIN      
        1 tttttctcgt caaggctggc gcgtcatcgc cactatgcga aatttgaaaa aaggagaaga
       61 cttgaaggac ctgcccaatg tggtcatcat gcccctcgac gtgtcagact cacagcagat
      121 taaggaaacc tgccaaaaag ccctggccga ctacgacgtc gacgtcctcc tcaataacgc
      181 cggctacggc gtcatggcac ccctcgaacg gatgcccgaa gatgctatcc ataaggtatt
      241 cagcaccgac gtcctcggca ccatgctggt aacccagcaa ttcattcctc attttaaaaa
      301 gcgcagagcc ggtgccatcc tgacgacgac gtccctggcg gccatcatcg ccctcccgcg
      361 agacagcgtc tacggcgcag ccaaacgggc tcagcagggc atgctcgagt ccttatacta
      421 tgaaatgaag cccttcaacg tcttcgtcaa agccatgatt cccggcggta ccaagaccaa
      481 cttccagacg cccatcaacg accgcacggg ctatgaagaa gcggctgccc accagcagga
      541 atacctcctt cacggcaatg aagaatttcc gggcccggaa gaagcggccc aagtcatcta
      601 cggagcggct accgatgaaa aggatcagat caattatccc accgattccg tctgccaggc
      661 cctttatgac caatacaaat ctatggggct cgaagaattc aagaaatact tcacaaaact
      721 cttataccgt caatagacgt agacatatag ctgcgtacgt catcttctga tgcccctaag
      781 acacaacacg gcccccaacc attttcggtt gagggccgtt tttcagtcat atattaaatt
      841 gtattccgag caatatcgag ggacttaata ttccggttcg atgaggccgt atttcccgtg
      901 tttccgttta tagacgacgc tgatcgtgtc ggtcttggag ttgaggaata cgaagaagtc
      961 gtggccgacg aggttcatct gcaaaatagc ttcttccaca tccatggggc gagcggcaaa
     1021 tttcttgaca cgagccactt caaaggattc ttccggtaca gccgggcctt cgatgagggc
     1081 ggcgttgaag gtacctttct tcatgcggcg ggcgattctc gtcttgtatt tatgaatctg
     1141 gcggaccagc ttatcgtaga ctaagtcgat agcagcatac atatcgtcgt tgccttcgac
     1201 accgcgaagg acgacgccgt ctactttcaa tgtaatttca gcgactttac gatctttttc
     1261 tacagaaagc agggcctgga cattgacttc gttatcaaag tatttcgtaa ctttcttttc
     1321 ctgtttttca acgtggtctt tcaaagccgg tgtaatttct aaattcttac ctctaacgat
     1381 ggttgccata aaacatccct tcctttcgtg gagagagccc gtttggaatg taactccatt
     1441 ccggcctccc ttatacttat agtattcaac aaggaaaggc gaaatccttc tcaaaaaaca
     1501 gaaaaacttt acattctttc gaaaaatttc taaggacgca aaaaaggccc tgtccgaaga
     1561 cagagccttt tgattgtgaa acaagcaagt atcggtaatt ctaaagatt
//
LOCUS       DAWDXZ010000437         1607 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12666_length_1607_cov_5.570232, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000437 DAWDXZ010000000
VERSION     DAWDXZ010000437.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12666_length_1607_cov_5.570232"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1216..1225
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          412 a          390 c          374 g          421 t
ORIGIN      
        1 aatgcactat taagtaaggt actatcccaa agggaagcat tgacttcatt actctctttt
       61 tgtatcattc taatagaaag gagtgtataa cgatggacga aaaattggca gcagtcaatg
      121 tcgaaccttt tgcctacgcc tgttccttat tcaacggaaa atggaaaatg cacatcctct
      181 tttggctgtg gaaaaaagaa gtactgcgct atggcgaact aaaaaaagcc ctgggaaagg
      241 taacacacaa aatgttatcc cagcagctca aggaaatgga agcagacggc atcatttata
      301 gaaaagaata tccccaggtg ccgcccaaag tagaatatgg gctgacaaca ctggggaaat
      361 ctcttatgcc tattttacag caagtttgta cgtggggaca agaccactat cagcgctgaa
      421 taccgaaggc ctggaatccg ggataaatcc cttttccgcc gagttcgtct tcgatctgca
      481 gcaggcgatt gtatttggcg acgcgttcac tgcggttcgg tgcgcccgtt ttaatctgtg
      541 ccgtattcaa agcgacggcc aagtctgcaa tggacgtatc ttccgtttca ccggaacggt
      601 gggatacgat agcccggtaa ccggaacggt gagccaattt aatggcttcc agcgtttccg
      661 aaacggagcc gatctggttc aatttaatga ggatagcgtt gccgctgccc tgagcaatac
      721 ctttggccag gcgtttcgta ttggtaacga ataagtcgtc accgacgagc tgcacgcgct
      781 gtcccaaagc ctcggtcagt ttagcccagc cttcccaatc ttcttcgtca agaccgtctt
      841 cgatggagta gatcggatat gcatcggaca gtttcttcca atgagcgatt aactcatccg
      901 tcgtgaaggt ctgaccgctc ttgggcagat ggtactggcc ctgtttttcg cctttccatt
      961 cgctggcagc tgcatcaata gccaatacga aatccgtacc cggtttgtag ccggcttctt
     1021 cgatagcttt caggatgtac tgaatagctt cttcatcgct ggcgagattc ggagcaaaac
     1081 cgccctcgtc accgacggaa gtagccaggc ctttgctctt caagagggca gccagtgcat
     1141 ggtagacttc agcgcaccaa cggaggcctt catggaagga aggagcgccg gccggcatga
     1201 tcataaattc ctgaannnnn nnnnntatca aaaggatcct gtcctttcaa tacgtcctgc
     1261 aataccgtgt tgatgttttc aacggctttg gttacccctt tgccgccata acggccctta
     1321 tcgccgtctc gaagttcaag agcttcaaac tggcctgtcg atgcgccgct gggagctgcg
     1381 ctgcggccta ctgtgccgtc agacaaaata acgtcagcct gtacggtggg gttaccgcga
     1441 gagtccaaaa tttcacgacc aacaactgct tcaatacgaa tgttcttcat tattcaaagc
     1501 cctcctctat atacataaaa aattaagtaa attctatagt gctatttaat ttggttatat
     1561 tatactatag atgtcatgat tttgcatagt actttttgag aatcttt
//
LOCUS       DAWDXZ010000438         1607 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12661_length_1607_cov_8.043814, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000438 DAWDXZ010000000
VERSION     DAWDXZ010000438.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12661_length_1607_cov_8.043814"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          363 a          348 c          445 g          451 t
ORIGIN      
        1 tacagggacc catattgcag tttttacagc atacgccggc aaggccgaag gtacactgcg
       61 gtttttgctg gtcgaagcgg tcaaaggtcg tgtcacagcc aatctgttcc atgcgcagca
      121 gcatttctcg tacggccggg tccggcgtat ggtccaggac atcctgcttg gaggcaaagg
      181 tcttgcggta ttcggcaacg gccttcatgt agtcattgac accgccgtga acgtggggat
      241 ggttgtgatc gtggggatgg acatggtcgg ccagacagtc atgaccatgg tcgtgatggt
      301 ggtcgtgatc gtgatgatgg tcgtgatcat gatgatggtc gtgatcatga tggtggtcgt
      361 gatcatgatg gtggtcatga tcatgatgat ggtcgtgatg gtggtcatga tcatgtttat
      421 gttcatgatg gtcgtgatcg tggtgtttat gttccttttc caattcttct tcggacatga
      481 ggacactccc cttctaaaaa taaaaaatat ataataaata ctttcattta ataaagcaaa
      541 tatcgacttt gcctaatgaa aaaatgaact gccttcaatt ataaaagcag ttcatttttt
      601 tggcaagatg gtgttggcta gtttttttta gaaatttatt ttactttttg ctggatgaag
      661 cccggcgtcg tgaggtaacg gtcgccgtta tccggcagga aggctacgat ggtcttaccg
      721 gcattttccg gacgtttggc aacttccgag gcggcaaaga gggcagcacc gccggagata
      781 ccgatgagga ggccttcagc ttcggtaaat ttctgagccg tttcaaaggc ctgttcgttg
      841 ctgaccgtga agacttcatc gtaaatcttc gtatcgaggg tttccggaac gaagttagca
      901 ccgatgccct ggattttatg cgggccggca tggccttcgg agaggagcgg agaatccttc
      961 ggttcgatag cgataatctt aacggacggt ttcttttctt tgaggtactg gccgacgccg
     1021 gagatggtgc cgcctgtacc gataccggcc aagaagatgt cgagaatttc tttggccaat
     1081 tcgttggctt tgtcaacggc accctgcatg cccgttttcc ccggagtcaa gacgatttca
     1141 gcaccataag cggagatgag gttgcggcgt tcgacggaca tcgtttccgg catggtaaag
     1201 atggcacgat agcctttggc ggcggcgacg gcagccagac cgataccggt attgccgctg
     1261 gtcggttcga tgatgaccga acccttcttc aagagccctt tcttttcagc gtcttcgatc
     1321 atggccttgg cgatacggtc tttggcagaa ccggccgggt taaaggcgtc gagtttaacg
     1381 agcagattgg cggaaatgcc ggcggcactg ttgtagcgtt ccggtttcaa gatcggtgtg
     1441 ttgccgacta actgtaacat agacgtgtaa attttactca tgatacatct ctccttttcg
     1501 tatggattca tcccccgatg aatgcaattg tataaacagt aaatgataag ctataccgaa
     1561 tgggtatata ttatatgtaa gcctgtgccg ggaaaaattt cctgtac
//
LOCUS       DAWDXZ010000439         1604 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12688_length_1604_cov_10.258231, whole genome shotgun
            sequence.
ACCESSION   DAWDXZ010000439 DAWDXZ010000000
VERSION     DAWDXZ010000439.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12688_length_1604_cov_10.258231"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1103..1112
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          372 a          406 c          446 g          370 t
ORIGIN      
        1 gccggaggca ccttggtcct gcggattgaa gatattgatg agcagcgttc caaggctgtg
       61 tatactaagg cacttatgga agacctttcc tggctgggcc ttgcctggga cgaaggcccc
      121 gatgtgggag gcccttacgg tccttacgtc cagcaggaac gttacgaccg ttatgacgag
      181 gccttggacg tcctgcggga aaagggactc ctttatccct gctactgcag ccggacgcgg
      241 cttcaggcca ttggggcccc tcatgccggc gaacataccg tgtacgacgg ccattgttac
      301 ggcctaagtg aagccgaacg ccgccgcatg accaagaaac cgtcgtggcg catccatgta
      361 ccggacgaaa ccgtttactt taccgactcc gtttacggcc ggcaggaaga ctatctgccc
      421 gactgctgcg gtgacttcgt cgtccgccgg gccgatgaca tgtatgccta ccagttggct
      481 gtttccgtcg atgatggcag catggccata acccatgtcc tgcgcggtga agacttgctg
      541 tcatcgacgg cgcagcagat ttggctcatg aagattttag gctacgacgc gccccactat
      601 acccacgtgc cgatgctcat cgacggcgat gggaatcgcc tgtcgaaacg acaaaaaggc
      661 attaccgtcc gcgccctgcg ggacgacggc gtcgatgccg agacgatttt atcccacctg
      721 gcctatgccg gcggcttggt tccggaacgg cgccgctaca gccgggctga attagtggcc
      781 ctttgtgatt tcaaaaaaat gcatacaggt ccgattgtta ttaccggaac gatgaagtaa
      841 atcggtttac taaccaatat atttaaaaaa gtaggctgtc gccaaagcgg cagcttattt
      901 tttttcccat ttcttgtcaa cattattgtc acagagtgtg taaatgtggt agaatgtggt
      961 ggagagtgaa aggagtgacg cgcctaatgt ttatgggaga atacgaacat acaatcgacg
     1021 ctaaaggtcg cgtcattttg cctgtcaaat ttcgggatga actcggtcca accttcatcg
     1081 ttacccgagg tttggaaggc tgnnnnnnnn nncacgatgg aagcatggat gcggtttgcc
     1141 ggaagtttaa aacaactgaa ggcatcgaag gaaaacgtcc gtgccttcca gcgattcgtc
     1201 tttggcagtg ctgctgaagt cgaattcgat aagcaaggtc gtatccttat tccggctact
     1261 ttgcgggcct atgcgaaact ggttaaggat gtgaccgttc tggggacagg agacaaggtc
     1321 gaaatttgga gtaaagacgc atacgaagcc tatgttgcca agactgttcc ggccatggaa
     1381 gaaatcgccg aaagcctcga cgatttcctg aacctggact tttaggagga attatggatt
     1441 ttcaccacgt cagtgttctt cgtaaggaaa ccatagccaa tttaataacc gacaccagcg
     1501 gcgtttacgt cgactgtacc ctcggcggcg gcggccattc cagggcctta gccgatcagc
     1561 tgacgggtga ggcgacgatt atcggtctcg accaggaccg ggac
//
LOCUS       DAWDXZ010000440         1593 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12783_length_1593_cov_7.718466, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000440 DAWDXZ010000000
VERSION     DAWDXZ010000440.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12783_length_1593_cov_7.718466"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          433 a          331 c          428 g          401 t
ORIGIN      
        1 agacatgatt gctgccagcc gtgcgttggg ccaaattgat gtaccctacg gtgtttatta
       61 cgtctttggt aaccatgaca agggacgcta cgccaaaggg cagcgcggat acgacggcga
      121 tgacctcatt gccgaactgc aaaagaacgg cgtgaccgta ttgcaggacc aaagcgtctt
      181 gatcgacgac cgcttttatc tggtcgggcg gcaggatgcg tccgaggaaa aagatttcgg
      241 cggcggtcgt gctgctattt ccgatttgac aaagaacctg gacaaagata aatataccat
      301 cgtcctcgac catcagccca gtgactatga ggccgaggcc gcagccggcg tagacctagt
      361 gctgtccggc catacccacg gcggacaact gatccccatg gggcagctca tgcgctggtt
      421 ccatatcgga ggagaggata atgtctatgg ctttgagcag cgaggaaaca cgaattttat
      481 cgtgacctcc ggcatttccg attgggccat ccaattcaag accggaaccc ggtcagaata
      541 tgtgatcatc gacattcaag gaaaatgatg taaaagagaa caatacatcg acccgattac
      601 gggcagtaag acatatagac aaaacaagag ccccgtgttg atgtgatccc aaaaagttag
      661 acctaccacc gtaacagcaa tgttgcggtg gtaggtctat ctagcagaat ggcgaagtcg
      721 gtattctacc ggctgcacag gtggttatga tgtatcaata atgttctata tttattatta
      781 atattttttc attaatgaac atagcatgga tttttatagt atacttaagg taattcaaaa
      841 ggagatgttg cctatgccgg gagaaatgta accggtccct ttatgtgtgg ctgtacgcca
      901 ccggattata agtactgccc gacttgtgta tttgcagaag ggaagccttt gttgagtact
      961 tgcatgatgt atccgggagg gatgatgctc aagccgaatc atgtgtataa cggtaagcct
     1021 tgcgaatggt atcagaagat agaatgaaag catttgccat gaatagagcg tattctgacg
     1081 ttagacgtag tgctttcgct tttggagacc gttcagagtg gctatactgc ctttgttttg
     1141 tgaacgggac gggcttatgg acagaaagga gtggtgccta tgccgggaaa tgtgacggga
     1201 ccttttatgt gcggctgtac gccaccggat tataagtact gcccgacttg tgtatttgca
     1261 gaaggtaagc ctttgttgag cacttgcatg atgtatccgg gtacgtctat aaagccgaaa
     1321 gtagtggacg aaggcgggcc ctgcgaatgg tacatgaaga tagactagaa gaatttatca
     1381 tgaatggaga tgctgcttat gccgggaaat gtgaccggac cctttatgtg cggctgtacg
     1441 cagccggatt ataagtactg tcctacctgt ttgctttcag aagggaagcc attgattagt
     1501 acttgcatca tctacccaga tccttatggg aagccaaaag aggttgatga aggcggaccc
     1561 tgccaatggt atatgaaaat agaataaagc acg
//
LOCUS       DAWDXZ010000441         1592 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12787_length_1592_cov_8.355888, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000441 DAWDXZ010000000
VERSION     DAWDXZ010000441.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12787_length_1592_cov_8.355888"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          402 a          346 c          459 g          385 t
ORIGIN      
        1 agatggggaa aaatggcttt gccatttcat cctcggctgc ctatgcgacg gcaggtaaaa
       61 ttttatttct cggttcaaat gctggcctac tcgtcggcgg catgatcggc tattccttag
      121 ccagtgcttg ctatggggag ttgatggcgg ccttgaaaga agccaaactt gccagggaag
      181 aacgaatccg gatagagcgt gaatgtgagg aagcgattgc agccatacga caataccgcc
      241 aagaaatcaa cgaaattgcc gatagacacc ttgcggaata tagacatacc tttgatgcgg
      301 cctttgccga tatgaagatg tccatgggaa tcggagatat cgatggcttt atggccggag
      361 ccaatacgat tacgcgtaaa tttggcggag aaacgaaatt tgattcattt gaagaatttg
      421 acaggttgat gaacaatccg gaacccttta aattttgatg aaggaggaac tgactatgcc
      481 tatccccgtt ttaattggtc ttggacttgg tgtggctgcc ggcgttaccg gcgttgtaaa
      541 ggccgttggt gccaaacaga agaatgatca agcgcaggat gtaaacgaga aagcccggaa
      601 gatttacgac gatgccaaag gggctgccga tagagctcgt cggacggcga acgaatccgt
      661 taccatatta ggcaaagaac gcgcggatac cttaacgaat gagtgttccg aatttaggga
      721 tacgttcaat aagatacatc atatagaaga ttctttttcc gtcgattttg gtgggcagct
      781 ggaatggaat gaagaagcgt atgttgaaat gaaaaaattg gaaaccattg cattttctat
      841 ggcatccgga gccctcaaag gttcggcggc gggggcatta acggcctttg gtgcctatgg
      901 cggcgtcatg tcttttggtg cggcgtctac ggggacggcc attgccagct taagcggagc
      961 agctgccacc aatgcgacct tagctttctt aggcggtggg acactggctg ccggcggtct
     1021 cggaattgcc ggtggaacca tggtcttagg cggccttgtg gcaggtccgg ccttggccgt
     1081 tttaggcttt acgttaagtt ccaaggcgtc ggctaactta gataatgctc gcagcaattt
     1141 ggccaaggct cgtgaaattg ctgaagagtt ggcggtttta agagatggct gttataagat
     1201 ccgcgatgtg gctcagatgt atacggacat gatccataag ttaaggcctc tcttatcgtc
     1261 tttgacggag gagatggact ggggaatccg agaatacgga accgattatc ggagctatcc
     1321 ggcggatgtc aaacaaaccg ttgccgccgg tatggcgact ttccaagcga ttgtaccgat
     1381 tgtccggacg gccataatta ctaaagacgg agccgtcgat gagagggctt tgcggattat
     1441 cgacggagcg aaggccaaat taccggaatt aaggtcatgg cagccagcat gaattttccc
     1501 tgcatccggt gcggcgaatg ctgccgccat gccggagaag ttccccaatt agctccgaga
     1561 acagatgccg acggcgcctg cctgtatctc ga
//
LOCUS       DAWDXZ010000442         1590 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12804_length_1590_cov_8.001954, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000442 DAWDXZ010000000
VERSION     DAWDXZ010000442.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12804_length_1590_cov_8.001954"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          379 a          395 c          403 g          413 t
ORIGIN      
        1 agatctccgt ttatgtcttt ttttatatgt ccgaattcat tatacaatca accccgcaga
       61 aaaaatgaaa aaataaagga agtcttatgg tgaaaaaaat atgttcctgg atcattggcg
      121 tcatccttac cgtcttggtc ctcttcatcg gtgtgttttc cttttggtat ttcggttcga
      181 ccaactatca tctgaccggt gtgcctgtct tgaactatca ccaggtaaat aataagttca
      241 atacagtcct gaccatgaag cctgccgatt ttgaacaaca gataaagtac cttcatgaca
      301 acgactacca tgctattacc ttggaacagt tcgacgccta tatgcggggc gaaggcgacc
      361 tgcccgaccg gccggtcctc attacctttg atgacggcta cgtcgacaat tatgaagacg
      421 cctatcccat cttgaagaag tatcacatga gggggacgat cttcctcatc atcaacctcg
      481 tgggaacgcc gggctatctg acttgggacc aggttcatga aatggcggct gacggcatgg
      541 aattcggatc ccatacgatg agtcataagc ccttgaccag ctttgaccgc cagggcgcac
      601 ggtatgagct gcaggcctct aaagccgcca tcgaaaaggc gacgggcaag ccttgtgagt
      661 tcatcgcctt cccggaaggg aagtataatg atatggtcat ggaggaaacg aaagaagccg
      721 gataccgcta tgcctttacg gttaatacgg gccgcgattt tccctgggac gacccttatg
      781 acctcgaccg cgtcccgctg tttgagggac ctatcagttt caaccatttc cggttccgcc
      841 tgaccttcag tgcctttagt gctcttttgt ggaagagcca ccaatatttc agccatatcg
      901 aaatcaccaa agatttggcc cagcacattc ccgaaccata gtaggaacct tctttgacaa
      961 tgaaaccccg gtttcaggta catcctggaa ccggggtttt tgtctgtgtc gtctacagtc
     1021 tgcccgtaag gacggcatcc tttaggcgtc ttacgttgtc tgtatcgtat tcgatttgtt
     1081 ctgaccagcg gcggagggct tccggaagat cgcccgaggc ggttgccgta agggcgataa
     1141 aatccgtatt gcgggctgca tagcaggcaa gggtttctat gggggaatcg gcaaaggttt
     1201 ccagctgacg ataaaagata taatcgtata tccgctggta taagaccttg tcacgtgtga
     1261 tggatgcctg tacggtttct ctttccggcc gcttcgtttt tagtgccata agcgctgctg
     1321 tccaagcctc gtcgataggt tcggtttcag aaaaaatagc taagagacgt tcgctggcgc
     1381 tttctgatgg gttgggctca taaatcagga ggtcttttgg caggtcgcag ccaatatcct
     1441 gtaagaggct gcccaaggtc cgaagcgtcg acgggtcctc actgtcaaag aaataaaggg
     1501 gagcacgttc ttttagcagc agttcgcagg ccgcttcaca ggcaaggccc aggccgccga
     1561 gttggtactc ttggccggca tcgtcggtaa
//
LOCUS       DAWDXZ010000443         1588 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12819_length_1588_cov_7.257012, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000443 DAWDXZ010000000
VERSION     DAWDXZ010000443.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12819_length_1588_cov_7.257012"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    446..455
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          351 a          397 c          456 g          374 t
ORIGIN      
        1 ttacaaggga atcttactat cgtataatat aaagtataga taagaaatta actgatgggg
       61 gatttctcta tgataaaacg tgcttctgtc ctcgtgggga ctcttgtgtt aacactcctt
      121 ctgtccgcct gtgccagagg tccggcacgg actgacaacg atacccatcg ggaaaccctt
      181 gcccaaaatg aaaagaagcc ggtttcgacg gctgtcgtcc ataacggcca ggggaatccc
      241 cgccgcgtcc tcattgctta ttttacgtat cccgataata cagatgccca agccattcat
      301 tccaaggaat acgacgtcat gacgtcggcc agcctcaatg taaaagacgg tgaaatcatc
      361 ggaaacgatg ccatgattgc caacgaaatc gcccgggaaa ccggcggcga cgtcttttcc
      421 atcctggccg aaaagcctta ttccgnnnnn nnnnnatgat tcgtgatgaa aaggcggcgg
      481 ctgcccgtga tgaacaacgg gagctgaaaa gccacgtcgg tgatatgagc cggtacgata
      541 cggtcgtttt ggtctatccc atttggtggg atgccctgcc ggcaccggtt acgtcctttt
      601 tgacggaaaa cgatttttcc gggaaggcct tgtatgccgt ggcttcgtct ggggacagtg
      661 attttgtcga taccgtcgaa gacattgccg cactcgagcc cgacgcggac gtctatgaag
      721 gcttcgtcct ccgtcatgac gagatgggcg aaacgtccga tgccgttcgt cagtggctta
      781 agacggcgaa tttcatgaaa taaaaaactc tccggttaca ccgcttggcg atgcagctgg
      841 agagtttttc gtaaatagaa cggggatacc ggtcttagga atgacgatga tagaggatga
      901 ggccgccttt tttgcggtgg cccgtattcc acagcccgtt gaagtcgagc cagcgccagg
      961 agccgtaggt aacggctttg accatgaagt cgccgtcgaa ttcggtgtag cccgtcagta
     1021 agaaccagtg ccagtcatag aatttaaagt tgggattgtt gtgatgcagg accaggtggg
     1081 ggatgggcca tccggcgtcg atttgagcct tgatgacgtc tttcgtttcc tctgccgtct
     1141 tatcgccgga ccaggggtcc atggtgatgt cgtcgtcgcc gtggtcggtc aggaattggc
     1201 ccatgccgtc gatgtacatg tcgaggcggt cgatgccgcc ccagcgcgga cggagatagg
     1261 gcttcatgac attggtaaag gcgatgtagt ccttcttggc gacgtcttgc tggaccagtt
     1321 cgacggccga gccgtctgag agttcgccgt aaaagacgat catattcggg tcccagtagc
     1381 cgatatcctg aatgctgaag gtaatgtcgc cggccgagac caggcggccg ccggcctgca
     1441 tcgtatcggg gagttccgat tcaaagttgt tgatcaaagc gacgatgcgc tgatacatat
     1501 cggaggccat atacggcgct tccgggtcct ccgtttccgt ttccggagcc ggcggacaga
     1561 gggccatttc cttgacttcg tcggggaa
//
LOCUS       DAWDXZ010000444         1586 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12827_length_1586_cov_10.254082, whole genome shotgun
            sequence.
ACCESSION   DAWDXZ010000444 DAWDXZ010000000
VERSION     DAWDXZ010000444.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12827_length_1586_cov_10.254082"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          326 a          566 c          415 g          279 t
ORIGIN      
        1 gcttgccatc gagacccgca ccgacgacga tgaactgaaa caggccttag cctttctttg
       61 cgacccgggc acgacggcgg ccacgacggc ggaacgggcc tttttagccg ccatcaacgg
      121 cagctgccag gtccccgtcg gcgtctacgg caaggccaaa gacgggcagc ttaccttgtc
      181 ctgcatcatc gccgcccccg acggcagccg cgtcttccgc catacggcga caggccctgt
      241 ttctgacagc cgggcccttg gccaagcggc tgccaagacc ctcctcgact gtgggggccg
      301 ccaaattttg acagacctcg gcatcacccc tgccgaataa aggaggtttt ccatgaacgg
      361 cattgtatat ctcaccggcg ccggccccgg cgactaccgc ctcttgacct taaagggccg
      421 cgacgtcctc cgtctcgccg acgtcgtcat ctacgactac ctggcggatg aacggctcct
      481 cgattttacc cggcccgatg cagaaaaaat atacgtcggc aaaaaagccg ccgaccacac
      541 cctctcccaa gagaaaatca tcgacttact ccttgaaaaa gcccgtgccg gaaaggtcgt
      601 cgtccgcctc aaaggcggcg accctttcgt cttcggccgc ggcggcgaag aaagccgggc
      661 ccttcacgac gccggcatcc cctttgaaat cattcccggc gtgacctcag ccatcggcgc
      721 tgcggcctac gccggcattc ccgtcaccga ccggacagcg gcttcgtcct ttgccgtcgt
      781 caccggccat gaagatccga cgaagaaaga atcgtccatc cgttgggaca aactggcaac
      841 ggccgtcgat accttagtct tcctcatggg cgtccacaac ctgcccttga ttacgaaaga
      901 attaatggcc cacggaagaa gggccgacac gccggcggcc ctcgtccgct ggggcaccaa
      961 gcccatccag gaaacgttag tggctaccgt cggcgatatc gcccaaaagg ctgccgaaca
     1021 aggcttcaag gcgccggcca tcttcgtcgt cggcgacgtc gtatccctta ggccatccat
     1081 gcagtggttc gatacgaaac cgctctttgg cctgcgcatc gccgtcaccc gcacccatgc
     1141 ccaggcgccg gccctgaccc ggcagctgga agacttgggc gcatcgtgta tcgaagtccc
     1201 gacgatccgc atcgaacggc cgagcgacga ttatgccgcc ctcgataccg ccatcgacaa
     1261 gatttcaggc tacgactggc tgattttcac cagcaccaac ggcgtcgaca cctttttccg
     1321 ccgcctgtcg aaaaaaaatc tcgacagccg cgccttagga cgggctaaac tggccgccat
     1381 cggcagcgcc acggcagagg ccctcttaac acacggcctg accgccgacg tcgtcccccg
     1441 ggcctactgt gccgaaggac tcttcgaagc cttatcaccc caccttagag gcggcgaaaa
     1501 aatcctcatc ccccgcgcca aagaagcccg ctccttcttg cccgataccc tccgccgtca
     1561 cggggcctcc gtcgatatct gtgaag
//
LOCUS       DAWDXZ010000445         1583 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12856_length_1583_cov_7.998691, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000445 DAWDXZ010000000
VERSION     DAWDXZ010000445.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12856_length_1583_cov_7.998691"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    497..506
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          375 a          439 c          500 g          259 t
ORIGIN      
        1 tattggtaag cctggttcat atcgtgaagg acgatgatca ccgttcgccc ccatgtttcg
       61 ttaatctcac gaaggagaga tagaatccgc aattgatggc ggacgtcgag ataggtcgtc
      121 ggctcgtcga ggaggagaat ggacgcatcc tgagccaata agacggccag ccaggcccgc
      181 tgctgctcgc cgccggaaag gtgctgcatg gccctttttt catagccccg aagaccgacg
      241 gcgtcgatgg cacgatgaat ggctgcctga tcgtccttcg tcagcggcga caagagcttt
      301 cgataaggaa aacgccccat agccactaag tcggcgacgg gcacgtcttg aggaaggtcg
      361 tgagactggg tcaagaaggc gatttcccgc gacaaggctt tacgcgaaaa ggaacggacg
      421 tcgcggccgg aaatttcgat agcaccgccg gaaataggga tcatgcagtt tatggctttg
      481 aggaaggtac tcttgcnnnn nnnnnnctac gatggccgtg atcttgccct tcggaaggga
      541 cgccgtcata tcgtgaagga tggtcttgcc gccccgttcg acgacgagat gtttgatggt
      601 aatggcatcc acggctcaca cttcctttcg caggagaaat aagaaaaagg gcgctcccaa
      661 ggaggcgatg atgatgccca cgggaagttc cgccggtgcc aagagaagcc gggctgccgt
      721 atcgctcaag gtcaccgtcg ccgccccgaa aagggccgcg ccgggaatga tatagcggta
      781 atccgtgccg attaaaaggc gtaccatgtg cggcacgatg agtccgacga agcctaagag
      841 gccggcgaca ctgacggcac tggctgcgag aagggctgca atggccgtaa ggcccagacg
      901 ggctcgttcg acgttgaggc ccaggctgcg ggccatgtcg tcaccgagtt ggagaagatt
      961 gaggtagtac gccccgataa aggccaagac gaagccggca gcagcatagg gcgtaatgac
     1021 ggacacgtct ttccagctgc tggccgccaa gcccccgacc atccacaata aggcgccgtg
     1081 gacgcggtcg ctgtaaaaga cgaggattcc tgaaatgatg gctcccagga aggccgagac
     1141 ggcgacgccg gccaagatga tgcgcacgac cttgatgccg tccttccaag ccaagaggta
     1201 gatagctccg gcggcggcca tggcgccgac aaaggagacg ggcgtcatca aaaaggcgta
     1261 cgacgggacg acgatgagga cgatgaggcc gaagagaccg gcaccggacg agataccgat
     1321 gatatgggga tcggccaggg gatttcgcat gacggcctgc aaaatggctc ccgacagggc
     1381 caaattagcc ccgaccaaag cccccgtaag ggtcctcggc agacggatgt tccagatgat
     1441 ttggtccgtc gttcccccgc cgggctgcca taaacttcgg acgacgtccg acgccgtaat
     1501 ggccgccgcc ccctgcatga ccgacaggaa gatgccggcc gccgccaaga cggcgaagac
     1561 gaggagcatg agccagcgcc aac
//
LOCUS       DAWDXZ010000446         1581 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12870_length_1581_cov_5.557012, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000446 DAWDXZ010000000
VERSION     DAWDXZ010000446.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12870_length_1581_cov_5.557012"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    734..743
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          360 a          392 c          382 g          437 t
ORIGIN      
        1 gtacaccagg aaggtctttg acacgaccgc ctctgattaa tacaacactg tgttcctgta
       61 aattgtgacc aatgccagga atataagctg ttacttcgat agagttcgtc aagcgaacac
      121 gggcaacttt acgcagagcc gagttaggtt tcttcggcgt agcagtgtat acacgagtgc
      181 agacaccacg tttctgcggg ctgtttttca aagccggcgc attcgatttg gttaaagcaa
      241 cctgacgtcc tttacgaact aattggctga ttgttggcat atgggcacct cctctccaaa
      301 caattagatt tataatgtaa tcatcaacga gcagagctca taagatgata ttaccaaaag
      361 ttatttcaac aagcctacgg ccgctgcttt tactttgata tggcaggcca ctcccagctc
      421 tgccatggta taggtgcggt cgacaggaat gtcagcccct tcagatgctg caataagcgg
      481 ggctacgact tgttcatcac agtccttggc gatgaacaac tgtgtgactt cgtttcgttt
      541 cattgccttc aaggtttgct tggctccgac agttttgcgg acaccggcag ctacatctaa
      601 gctcacgaaa cctcactcct tacgtcttgc aaattaggcg caatgcacgc attgcgacac
      661 ttagatattt tatcatcacg ataattatct gtcaatgagt tttttggtaa aaactgcgta
      721 accagtacat cttnnnnnnn nnnttcaggg gcatctacgg gattgccgtc ttcatcttca
      781 tagctgatgg aggggacggt ctttactact tttactttgc ggtagcggct catgccggtc
      841 cctgccggaa tgagtttacc gataatgacg ttttctttaa ggcctaagag cggatcgacc
      901 ttacctttaa tagccgcatc ggtcaggaca cgagtcgttt cctggaagga agcagccgac
      961 aggaaggaat ccgttgccag ggcggccttg gtaatcccca gcagcatcgg tttgccgata
     1021 gcggtcttgc cgccgttgtt ggcaatgcgc ttattcatat cttcaaagat attgacgtcg
     1081 atataatcgc ccggcaatac atctgcatct ccggcttctt caatcttcat cttgcgcagc
     1141 atctggcgaa cgatgacttc gatgtgctta tcgttgatac caacaccctg tgatttgtat
     1201 accttctgta cttcgtatac caggtagcgc tgtgttgctt cgacgccctg gatacgcagg
     1261 atatcgtggg ggttgataga gccggccgta atacggctgc ccttttcgat gacatcgcct
     1321 tcatgcacga cgagatgcga accgaaggga atggtgtaac tccgttcttc gccgtctttc
     1381 gagaggacgt gaacgatgtt gatgtcgtga tggtcttctt tttccgtaat cgatacggta
     1441 ccgctgattt ccgtgacgat agcgttgccc ttcggcttac gagcttcgaa cagttcttcg
     1501 acacgaggaa gaccctgggt aatatcgccg gccgatgcga cgccgcccgt atggaaggta
     1561 cgcatcgtca gctgtgtacc c
//
LOCUS       DAWDXZ010000447         1579 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12888_length_1579_cov_7.316273, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000447 DAWDXZ010000000
VERSION     DAWDXZ010000447.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12888_length_1579_cov_7.316273"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          411 a          322 c          428 g          418 t
ORIGIN      
        1 tgtattatag caaacagtgc ctttaaggtc aacttattga attaaaatca tggtttacaa
       61 ttcgtctttc agtaatttcc ctaagcaggg ttcgacttgg acgccttcac ggggatcgct
      121 tccggcttta tgggtatatt cgatggcata gctcaggaag tcagcagccc ttttgatggc
      181 ggcatggagc atcgtccctt ggagcagagc gccggtaagg acactggtaa agaggtcacc
      241 cgttccacag gtgctgaagg gaaggcggtg gaccggatat tcgtcaaagg aggccgtcgt
      301 cgtatcgtaa ctgacgattt tggtggtatc taagtcgctg gcgacactgg tgatgacaat
      361 ctttttgggg cccatatcgg agagcgtttt gcacaactca agcatttccg tcgtcttcgg
      421 aaggtcttca ctgtagggaa ccccggctaa taaacaggct tccgtataat tcggtgtaat
      481 taccgtggcc ttggcaatga gttttcgcat ttccggaacg atagccggtg tgaagaccgg
      541 gtagagattg ccgttgtcac ccatggccgg gtcaacgacg atgagactgt catctttggc
      601 aaagcgggaa atggcatcgg aaacgacttc gatttgaccg gcgtcggcta aaaatccgct
      661 gtaaatggcg tcgtattcat agcctaattt ttgccattta tccataaact ccgtcatatg
      721 gggcgtaaaa tcggaaaatg tgaaatcgcc gtaggtcaga ttattgctga aaatagccgt
      781 tggaaaaggg cagacctgaa atcccatggc ggagatgacc ggaattgccg ccgtaagtga
      841 gcagcggcca aaagaacata aatcgtgaat cgcaaggacg cgtttggttg tcatagaaag
      901 acctcctcta agtaaactgt actataattt taacctatat actgcaaggt gtaaataaga
      961 cagataaaag ggattctgtc ctctatgcac tggtatttta aaggggcgca gagggctttt
     1021 gtggattttc ctgtaagctt attggcgcgg ttatgatata attaaaggta atcgaaattt
     1081 tcaaactgta ggcgaagtct tgatgggact cagtgtcaag gcgatggtct ttcacgacgg
     1141 caagctgctg ctcttgcaga aaaatgatat agaaggccgg catccttggg aatttcccgg
     1201 aggaggactg ctctttcatg aggattttga agtcgggctc cggcgggaag ttcaggaaga
     1261 aacgggtctg tccatcacct tggaagcgcc ggccggtatt tggagctacc agaaggctga
     1321 cgggcaattt ttaaatggcg tcatttttac ggctatggct gatgcggagc aggttgtcgt
     1381 atccgatgag catcgggatt atcgttgggt gaagccggaa gaactttcgg cctatcgcct
     1441 gcaggaatcg ctgcagcaat cattgaaaca gatgaaacaa ttttcttatg agaaaagtca
     1501 tgccttactg cgcggctttt tagaccatat atgatatgag gtttttatga atagagaaca
     1561 acaacagcgg gctcctttt
//
LOCUS       DAWDXZ010000448         1577 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12903_length_1577_cov_15.109067, whole genome shotgun
            sequence.
ACCESSION   DAWDXZ010000448 DAWDXZ010000000
VERSION     DAWDXZ010000448.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12903_length_1577_cov_15.109067"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          477 a          340 c          378 g          382 t
ORIGIN      
        1 gcctacatgt tgcggcaact gctcgccctg caaaggcgga aacgggaaca cggcgggcaa
       61 cggccgtgaa aatacgacgc gaaaaaaatc ggacgataac tgtactgcac cccaaaagtt
      121 ggacactttt ggggtgtttt ttatgaaata taattatgag attcgtttaa aggccgtaaa
      181 actggtactc gaaggcggac tttcggttag ggaagccgga tgtcacttgg gctgtggccg
      241 ctcgcaagtt cacttgtggg taacattatt cgagcgccat ggccttgccg gccttaagct
      301 gcgccacggt agctacagtg cagaatttaa gttgtcagtt ttgaagcata tgcaccaaaa
      361 tcatctatct ttgctggaga cagcagtgca tttcggcatt ccgggccctt ttgttattcg
      421 tcaatgggag cggctctatc aaaaccaagg tgctgaaggc ctgcggcgta aaccgcaaag
      481 aaggaggccg gccatgagta aatcgaagac taaaaaagtc aaactcaaaa cgactccgca
      541 cgaagagttg cttaaggaac tggagtatct tcgtgccgag aatgcttact taaaaaaatt
      601 gcaggcctta gtcgaggagc gcattgtccg cgagagcggg aaagagccca agccatcgaa
      661 ggactaaggc cgcagtgccg gctttcagta ttgctcaaag ccgcagggat ggcgcgaagc
      721 acattttatt atcacttcag aaaatcaaaa caacccgata aatatgctcg cgaaaaagag
      781 agtattataa ggttatatca cgaacataag gggcgttatg gttatcggcg cattaccgtt
      841 gaaatgaata agatcggata tgcgataaat cacaaaaccg tacttaagct gatgaatatt
      901 tgcgggataa aaagtcaggt caggctccgt aaatactgtt catacaaagg acagatcgga
      961 cggatagcgc ccaatcttct gcaacgcgac tttgcagccg aaaaaccgaa tcagaagtgg
     1021 gttactgacc ttacggaatt ttcggtttgc ggcgtaaagt tatatctatc gccgattatg
     1081 gacttatata accgggagat tattagctat aaaatagctg aacgccctaa ctttatgcag
     1141 atcatgaaga tgctggacga tgcgtttgcc agaataccgg attctccggg tattgtcttg
     1201 cattccgacc agggctggca gtatcagatg aagcagtatc agctccgttt aagacagaaa
     1261 ggtattacac agagcatgtc gcgcaagggt aactgtctgg ataacgctgc tatggaaagt
     1321 ttctttggat tattgaagtc cgaattatta tatttgcaaa aattctcatc catagaccat
     1381 ttccgaaaag aattggaaga atatatcgac tactacaata acaagcggat aaagaaatac
     1441 ttaaacaaca tgagcccggt acaataccga actcatgcca tctaaacaaa tactaatccg
     1501 tccaatattt ggggtgcact tcaaacaacc gtccgatttt tttgtttccc gtcaatcgac
     1561 cgccgtatgg tcgccca
//
LOCUS       DAWDXZ010000449         1576 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12915_length_1576_cov_7.205128, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000449 DAWDXZ010000000
VERSION     DAWDXZ010000449.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12915_length_1576_cov_7.205128"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          329 a          369 c          443 g          435 t
ORIGIN      
        1 ccggcccctt tcacggctgc aggtttattt cagtttacgg acagtacctt tttcttctaa
       61 tgcttttaag gtagctgccg ggtcttcaca tttcgccagc ataatcatgg cggcgatgac
      121 atagggttta tagctggcct ctttgtagag ggaatcgcgg taacgatcga atacggaggc
      181 ttcgacttcg ccgtcttcac agctgacgcc gttgatatcg gccggcaggc agtgaaggta
      241 catggctttg ccgtcttttg tcgtcttcat catgtcttcc gtacagcacc agtctttatg
      301 ctgggcattt tgcgccagca gctcttgttc taaggcatcg ataccggcct ggtcaccggc
      361 ggcgtagaga ttggttctct tttccatggc ggcaaaggga gcccagctct tgggatagac
      421 gacgtcagcg tctttgaagg catcggccat gtcgttggtg atgcggaagg atcctccaga
      481 ctgctgagca ttctttttgg cgacgtcgac gacgtcgggc atgacttcgt aaccttccgg
      541 atgggctaag acgacgtcca tgccgagacg ggtcatgagg ccgatgacac cttgaggaac
      601 ggacaagggt ttgccgtatg acggagagta ggcccaggtc atggcgattt ttttgccttt
      661 caggttttcc aggccgccca tttcgtggat gatatgagct gcgtcggcca tacattgggt
      721 cggatggtcg atgtcgcatt ggaggttgac taaggtcggt ttctgttcga gaataccgtc
      781 tttatagccc tgggtaacag cgtcgacgac ttctttttgg taagcatggc ctttgccgat
      841 atacatgtcg tcgcggatac caatgacgtc ggccatgaag gaaatcatgt tagccgtttc
      901 gcggactgtt tcgccgtggg caatctggct cttcttttca tcgaggtcct gaacctggag
      961 tcctaagaga ttgcaggccg aggcaaagga aaagcgggta cgcgtcgagt tgtcacggaa
     1021 gatggaaatg ccgagaccgc tttcaaagag tttggtagaa atgttatgtt cccgcatgaa
     1081 acgaagcgcg tcggctaaaa cccagatggc ttccaattcg tcgtcggtct tatcccaggt
     1141 gaggaaaaaa tcgttttcat acatttcttt gaagttgagg gcctttaatt tatcgatata
     1201 atcctgtaat gttttcatca gagtgtctct ccttttaggt gaattaaact tatttgctgg
     1261 tgtaaacgga tggtaaggca gcgtaaacgg cggcgcaggt cactaaatcc tgtttccaag
     1321 tcttttcatt cggagcatgg gcctgggctt ccgcgccggg accgaaaccg atgacgggga
     1381 tgccgttacg gcccatgatg gaaacaccgt tcgtcgagaa ggtccattta tccgtaaggg
     1441 gcagggcttt tcgcattttt tccgtttcag ccgaaccgat gcggctattt ccgaagaggc
     1501 ccgtataggc ttcttctaag gctgtcgtaa ctttgtgatc cttggggatg acccaggtcg
     1561 ggaagtagca ttcaat
//
LOCUS       DAWDXZ010000450         1572 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12938_length_1572_cov_9.328939, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000450 DAWDXZ010000000
VERSION     DAWDXZ010000450.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12938_length_1572_cov_9.328939"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          376 a          374 c          385 g          437 t
ORIGIN      
        1 ttcaaagggg taatgggatt tgatgcggat gccgtgggaa ttaaaaatca atgtatagcc
       61 ggcaatcccc gtcgatccct gataggcctt gcagaagccc ccgtcgatgc atatttcctt
      121 gccttcagct cgaacagggc tttcgccctt cttgacctta accggggtat ggccgttgat
      181 gatgtgaccc tgaggcgaat cgatgccgaa ttcatggagg atcttgatgc attcatcctt
      241 atctcggttc aagtggtaat aggcattttg cggttctttc caggtggatt catcgacgat
      301 atagcttcgt tcaagtgtct ttacgatgcg tcccgatacg ggtgagtcgg cgccgcacca
      361 cagataccac atgaagtcca ggctgtccat atcgcgctgg tcataggcct tgcggatcat
      421 atgctcgatc ttatccataa aggccttgcc cttatacggt ttgccttcga gatagacctc
      481 tttaaaactg ccgtccggat tgaggggcag accgccgtgg aacaagagat tgttgttcat
      541 gcagcgatac agtgacccat ggctgtaaag gaatcggata tgacgctgaa ggcgttcgct
      601 ttcgagaaat tccagttgaa tatcgttcat caattggtgt tcttccggcg acaaagtata
      661 cggatccgcg gaatttaccg tcggaaaatc cttggtcttc agttcgtaag tcttaccgtc
      721 taaatcaaca gtcccttttt cccaatcgaa tttgtggaac atgagccggt catccatatt
      781 atagtcggga tggcgataga tcgtttgtcc ttccagttta gctacgatga cattaatggc
      841 tttgaccatc ggttcgatgc catcattttc cttatatgtc tccatagcaa agagggcaaa
      901 gggacgtaaa ctgatgccat aaccgttttc caatatttcc atattatggt aacgaatgtt
      961 attgcgcagg acgttggcaa tgcaggccaa attgccggca gcggcgccca tccaaaggac
     1021 atcgtgattg ccccactgaa tatcgagcga atggtgtccc atcaaggtat cgacgatttt
     1081 atcagcatgg gccccgcggt catagatgtc accgatgatg tgcaggtggt ctactgccag
     1141 ccgcttgatg agggccgcta aggcatagat gaattgccgg gtactgccgg tctctaaaat
     1201 cgtatcgagg attttccgat gatagaccag ctggttctgg tcctcgtcgg gctgggcatg
     1261 aatcaattcg tcgatgatga agcggaaggc ttccggcgtg gccttacgca ctttagaccg
     1321 tgtatattta gacgacaata acttagccag gtcgatcatc ctttccagcg tcgtccgggc
     1381 ccattcactc gtaacctttc cttcacgata gagcatattc agcttttcct tcggataata
     1441 aatgagggtc aaaagactgt cctgttcgat tttattcatc ttatccgaaa aaatcatttc
     1501 taccttttcg cgaatgacgc cggagcagtt atttaagata tgatagaaag caccgtattc
     1561 accgtgaagg tc
//
LOCUS       DAWDXZ010000451         1571 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12950_length_1571_cov_6.717018, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000451 DAWDXZ010000000
VERSION     DAWDXZ010000451.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12950_length_1571_cov_6.717018"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          350 a          471 c          347 g          403 t
ORIGIN      
        1 agccccacat cctggtccgc ttcatggcca tcggcgaccc ggctgaatta aagaaatcga
       61 agcacatcgc catgacctgg gtcgtcatct ccctggccgc cgccgtcctc gtcggcctcg
      121 tcggcaaagt cgtcgtcacg acgcccttga ccggtgccga ttcggaaaaa gtcttcttgg
      181 tcatgagcgg tcagctcttc ccgccctttg ccacaggcct catcttgtcg gccgtcttag
      241 ccgccgtcat gagtaccgcc tcgtcccagc tcctggttac ggcatcggct atttctaaag
      301 acttctacca caccttcgtc cgcaagaacg ccagcgacaa ggaactcatt tacgtcagcc
      361 gtctgacggt cctcgtcgtc tcggctctgg ccatcgtctt ggccctcaac cccaacagtt
      421 acatcctgac catggtttcc tacgcctggg ccggtttcgg tgccgccttc ggcccgacga
      481 tcctcctctc cctgtactgg aagcagatga ccaaaaacgg ggccctggcc ggtatcatcg
      541 tcggtggcct gacggtcctc atttggaagc agttcaactg gttcggcctc tatgaaatcg
      601 taccgggctt cctcttctcc ctggccgcca tctacatcgt aagcaagctc gaaggcggag
      661 ccagccagga aatccaggac gaattcgatc aagccatcca tcacgcgccc aagatcagcg
      721 tcctccacag cgaagaagac taatcgcttc gaacgatgca tcaccatggc ctaacccctg
      781 ttttcagaca gccaaataca aatgcagtca cccaaaagga tgactgcatt ttttatgccc
      841 ttttacggct attgaccgct ctcaggatta tttgattgca acaggccggc tacgccctga
      901 ctcagcagtt tgttcgtaat gtcaatgacc tcttcgacgg gaagtttttt cccgccttcg
      961 acccattgac gatacatgga aagtcccgtc gcatggatga aggtcgtaaa cagttcctgt
     1021 tcgccagggc tcaactgctg aaaggccgga gccttccccc aactcttggc aatatattct
     1081 tccaccaagt gactgctcag gtgctgatat gatacggagc agaggatttt ttcatacagc
     1141 ggcccttgat ggacagcata atggaaaaaa gcagcgttga tcttatccag atcctcgggc
     1201 acccgataat cggcaatggt gtcgacatag cccttagaaa attcactgag tatttcttcc
     1261 aagagagcat cgacactatc gtagtagcga taaaaagtct ttttattaat cacagcctga
     1321 cggcaaaggg ccgtaacggt gatcttccga taatcctcct ttaagagcat gaccgaaaag
     1381 gccgttcgaa tcgactgaat cgtcttttgt acacgcaggt cttcatgacc gcttaacttc
     1441 atatgctaac ctcctttctg gctttccctt attgtatcat tttttcgtta ctttttcgct
     1501 acccttttta tataagtgtg gcataagtcc cattcagtcc catactgacc attgtcgatt
     1561 ccctttttcg a
//
LOCUS       DAWDXZ010000452         1571 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12952_length_1571_cov_6.258575, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000452 DAWDXZ010000000
VERSION     DAWDXZ010000452.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12952_length_1571_cov_6.258575"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    928..937
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          334 a          503 c          425 g          299 t
ORIGIN      
        1 ccgctcggtc tatggccggg ctgaagccga atatgtcatc aaaaaatccc gcttcatcgc
       61 caccgtctgt gaagtgaaaa cggaagatga ggcggcagcc ttcatcgaaa gcgtgcgcaa
      121 gcactattgg gacgcccgtc acaactgctc ggccttccaa atcggagccg gcggacagat
      181 ccagcgctcc agcgacgacg gtgaaccgtc gggaacggca ggccggccca tcttagaagt
      241 cttaaagaaa cgagacctga ccaatacggc cgtcgtcgtc acccgctatt tcggcggcat
      301 caaactcggc gccagcggcc tcatccgggc ttacagccac gccgcgaccc tggccctcga
      361 cgaagcgtcc gtcgtcgtct acacgccctt caccctcctt acagcgaccg tcgcctaccc
      421 cttggtcagc accctcgagc gctttgccga aagtcacggc gtcctcatcg ccgaccgggc
      481 ctttgccgcc gacgtgacct tcacctttga agtccccgaa ggcgacagcg acgccttcat
      541 cgccgacctg accaatgcca ctaacggccg cgtcagctgc aaaaaaacgg gtaccgtcgt
      601 aaaaccggta cccgtcacat agacgtaaca gcttcgatca ggccttatag cctgttgcca
      661 tatcgaccat attttcaagt ttttcgccgg cgaggaaccg cttcaggtta tcgacggcaa
      721 tgcgggcgat gtagtcgtgc gtcgcctgca ggtggtagcc gcctgaaata tggggcgtaa
      781 tgtagatgcc cttcgtctgc cacaagggat cgtcctcggg cagcggttcc ggatcggtaa
      841 cgtccaaagc agcccctgcc aaatgaccgg aaaggacggc ttcccgcagg gcttcctgat
      901 cgacgttgtt gccgcggccg acgttgannn nnnnnnngat ccgatacgtt tccggcgtat
      961 caggcaggca cgacgcgacg atgtccgccc gagagagata gtcttcgaaa tgagccatat
     1021 cccccatttc atcggcttcc ggcggaacct cgcccttacg gcggcgcagg ccgatgacat
     1081 aagcgcccaa agcctttacg cggcggccga agttgcggcc gatatcgcca aaaccgacga
     1141 cgacgaccgt ggccccataa aaggacgtga ccgggccttc atcgcgccac aaggattgat
     1201 gctgattgtc atggtagaga tacagcttct tcatcatagc caggctctgg gccagcatgt
     1261 gctcggacaa cgccaggcca taggcaccgg tcgcattggt gagcgccacg ccgtcgggca
     1321 ggataccggc cttacagtaa ctgccggcac cggcactgtt gagctggagc catttcaaat
     1381 gcctggcctt gctgaccagc tgcgggtcga tgttgccgat aatgacgtcg gcgtcttcaa
     1441 gggccgcacc ggcatcgctt ccatcaccgg gacagaaggt caccttggcc cctttcgcag
     1501 ccgtttcaaa ccactgtcgc tgttttgcat ccgtatccat gacaacgaga atattcaaca
     1561 gaatcactcc t
//
LOCUS       DAWDXZ010000453         1570 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12962_length_1570_cov_7.509571, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000453 DAWDXZ010000000
VERSION     DAWDXZ010000453.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12962_length_1570_cov_7.509571"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    936..945
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          329 a          434 c          452 g          345 t
ORIGIN      
        1 ttggcaacga cccagctcgg gaaaggcatc gacgaaacac gccatttgtc ggctgacgga
       61 atggaagcgt cttttgctgc catggaaggc tggaagaaaa agctttccgg cgtacccgtc
      121 tgtgccgtcg ctaccagtgc cgttcgtgaa gccgttgacg gccaggcctt tttggcagaa
      181 gtacgagccc gtttcggctg gcactgccgg gccatttcag gccttgaaga ggcgtccctc
      241 tgttttacgg gagcgacgaa gtttatcgat cccgaaaaga cggcggccat tctcgatatt
      301 ggcggcggca gcagtgaagt cgccgtcggc aaacgaggga ttttatgctg gtctcacagt
      361 tatgccatgg gcgccgtccg gttcacgaaa aaggacgtcc tcaaggaaga cgacgtcctt
      421 cacctggaaa accgatgtca taagatgtgg cttcccatcc ctatgagtga gacgccgcaa
      481 gtcctcatcg gtgtcggcgg taccttgacg acgttggccg ccatggattt aaagctcgac
      541 gtctacgacg ccaaaaaggt cgaaggtcac gtcgtgtcgt atgaatcctt gtgtcagcac
      601 attgagaaac tgcgtgtcat gacgccggaa gaacggcgcc atgtaccggg ccttcagccc
      661 aagcgcagcg aaatcatctt agccggtcta atcattgcca agtcgttctt aaagcgctat
      721 gaaattccgg ccgtcatggc cagtgaacgg gacatcatgg agggcatctt ctttcgtcat
      781 tcctttcacg atgcggcctg gaactcgaag gaaggccggt tatgagcctc gtcgaagacg
      841 ttgcggacta caaccggcag cacagcttgt ggcagccggg aagtgccgtt ctcgttgcct
      901 gttcgggcgg accggattcg atggtcctcc tcgatnnnnn nnnnncggca ttcgggctgc
      961 tgctgatgcc gaagtcgact ttgttcgccg cgaagccttg aagcgtcatt gcggttttgc
     1021 ttggcagtac gtcgatgtgc cggctctcgc ccgtcgtgaa aaaatgtcag aagaggcggt
     1081 gggacggcgg gaacgctacc gaattttacg cgctgtgaag cgggcctgcg gcgcttcggc
     1141 gattgccgtc gcccatcacg gtgacgatca agccgaaacg gtattgctcc acctccttcg
     1201 cggcagcggc cttcaaggcc tgtgtggcat ggagccgaga aggggggaca tcatacgccc
     1261 gttgttgggc tttacgaaag ccgagcttgc ttcatatgct gccgaacaag ggattccctc
     1321 ttgtcacgat gaaaccaatg acagtacacg ctaccaccgc aaccgcattc gcttagagtt
     1381 actgcctgcc ttaacacgat ataacccggc cattacggcc gacctcaacc gcctgtctga
     1441 tacagtccgg gcggatgaag cttttttaaa ggactgtacc gagcaagtct atcgggatat
     1501 tgcctgtccg gatcacggcg ttccggccct ggacagacgc aaattactgt ctcagcctct
     1561 cgccttgcag
//
LOCUS       DAWDXZ010000454         1567 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12983_length_1567_cov_6.387566, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000454 DAWDXZ010000000
VERSION     DAWDXZ010000454.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12983_length_1567_cov_6.387566"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1178..1187
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          448 a          422 c          308 g          379 t
ORIGIN      
        1 tccttccacc ataatgcagc ccggtcgttc aggttcattt ccacctttga aaacaatcgt
       61 gcaatgcccc aacgtacgaa gtgtctcttt ggcttcctcc ccgacagccg taatggtaaa
      121 gattttatcg ccaatgataa gcgtgtcatc cggagccggc acctgcgtca gattcccgac
      181 tgtatgaagt atcgaaatat cagccagctc ttctggagca ttttcattaa agagaatgat
      241 gaagtttaaa tccggttcct gcaaaaactg aagcgcttca tctccccagc ccgttacact
      301 gcagtgaaat ttcatagaca ttatctcctt ctataaattt atgaatacat gccaatactg
      361 gcaacataag caataacaac agcaatgggg cccgtcaaaa cgcgagaata caatacggcc
      421 ggcacaccta attcaatcgt ttccggttca gcttctccca aacttaggcc aaccgggacg
      481 aagtcacaac cggcctgtgc atcgatagcg aaaagtgccg gcagggcata ctgcggaggg
      541 atattcccca aaccgatctg agcaccgagt aatgttccga caacctgcgc aataacggca
      601 ccggggccaa ggaccggaga aataaacggc atggcacaaa tgaaggaaat gatcaacatc
      661 cccggaagcg tacttgcaac aggcgagatg acgtttgcaa tgacatcacc gataccggac
      721 gcttgaataa gccctaaaat catacttacg aaagccataa acggcaggat gctcttcaag
      781 gtgacatcga tggtttcacg gccggcagca taaaacttac tgatgatact gcctatgcca
      841 atgccaatcc gagtaaagat atttttcttg gcttccggag ccttttcctg tgcgggagca
      901 gccttcactt ccggtgccgg tgttgccggc gtttccacct tcgcagcttc cgcttcatca
      961 agataagcaa agcacgattc atcgacagcc gatacgtaga tatcttccgt aatatatttg
     1021 gctaagggcc ctgtcttacc gacaggcatt acattcaatg tattgattcg ttttttcgga
     1081 tagaccccgc agcgagccgt accgccgcag tcaacaacaa ccgctaagat ttcattatcc
     1141 ggcaccccgt tcgtaaatcc atcgataacg tcacaacnnn nnnnnnntcg gatttcccaa
     1201 aaatacaacc gctaaaatcg gcaaaatcgt gtatcgagta atcatatatt tcgtcgattt
     1261 ttcggctagc cgattgactt tgtcttcccc gatcaactta atcaaagagt tgaacgctgt
     1321 catcataacg ataaccagcg ggatgatccc tgcaacccat ccggcaaaaa ttgtcgcacc
     1381 ggatttaaat aattcaataa acgcatttgc aaaccaagca attgattcca taaaagccac
     1441 ctttctttct caaaatacta tccgttcagt taatctttcc gaatcccagc ggcaatcaca
     1501 cctcctggca gccgggcaat cccaatcgct cttctaaaac atcaagagct tgtatatacc
     1561 ctttata
//
LOCUS       DAWDXZ010000455         1565 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_12994_length_1565_cov_9.993377, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000455 DAWDXZ010000000
VERSION     DAWDXZ010000455.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12994_length_1565_cov_9.993377"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          274 a          448 c          456 g          387 t
ORIGIN      
        1 ccgatccgta gacgggaacg tagtaggcgc cgtcggcttc gagctgccag ggtgcggcgt
       61 ccggcataag ggccgtaaaa ccggccttgt cgagaaggtc ccgcgaagcg gcggccgtgt
      121 ggtacggcga cgggctttgg gcgatgaagg tgagtaaggt atcgatagag tcatgggaca
      181 tgacgatcat cctttcttgg ggataaattt tgtgatcctg ttattatatc ataggtcggc
      241 cacttgcgct tccggcaggg aacgcttggg cgtcggaaaa tcacagcggc ggccggcttt
      301 ttgttttctc aagccctcat ggtataatag agaaaaatta attttcatga aggggatctt
      361 atggatttac gcgtcatttc tcgattttta ggaaaaatag ccttggccca gggcggcgtc
      421 ctgttttggc ccctggccat ggcggtcatc atgggggaat cgagctggct gccttttgtg
      481 ggcagcatca ttctctgcgg actcgtcggc tgggcctgcc tgaactacgg ccacgtgcgg
      541 acgcagaaac tgaccatgcg cgaaggcatt gccattacgg gaatcggctg gatgctggct
      601 acgtttttgg gcatgcttcc gtactgcctc ggcggctacc tgtcgtattt cgacggcctc
      661 tttgaaagcg tgtccggctt taccgggacc ggggcgacgg tcattacgga ccttgaaatc
      721 ctgccccaga gcctgcttct gtggcgcagc atgacccact ggctcggcgg cctcggtatc
      781 atcgtcatct tcatcgccct cttgcccgaa gccggtcagt cggcggtctt ttacatgtat
      841 aatgccgaat cgacgggtcc tacgagagag cgcgtcctgc ccaggctgca cgacatgacc
      901 aaggtcctct ttcgcatcta catggccttt acggcgacgg ccttcgtcat cttcttcctc
      961 tgcggcatgg actttctcgg cgccgtcaac cacgccttgt cgacagtctc ggccggcggt
     1021 ttttcgacgt ataatgccag tgcggcccac tttgaaagcc cggccgtcga aggttggatg
     1081 acctttttca tgatcctcac cggcggcaac tacgccttgt attaccgggc ctggcaaaag
     1141 ggctggagcg tcctttggga caatatggaa tttcgggtct atctcttgat cgtagcggcg
     1201 gcgacgattc tcatcggcct caacctcatg agtgccttcg gcatcggccc ttcgacggcc
     1261 cttcgctact cgtccttcca agcggcgtcc atcgctacga cgggcttcgt ctcggccgat
     1321 ttcgaccgct ggccgacttt ttctaagggc attttgctga tgctcatgat ttccggcggc
     1381 tgtgccggct cgacggcggc cggccttaag gtgtcgcgcg tcgtcatcat ggcccagtac
     1441 atttggcatg cggcgaaaac gaagctccat cccaatcagg tcctgggcat gaccatcaac
     1501 ggccaccgca tcggcgacga tacggtcgtc cgcgtggctc agttctgctt cctctacgtc
     1561 atgtt
//
LOCUS       DAWDXZ010000456         1564 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_13001_length_1564_cov_9.353877, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000456 DAWDXZ010000000
VERSION     DAWDXZ010000456.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13001_length_1564_cov_9.353877"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          358 a          404 c          372 g          430 t
ORIGIN      
        1 gtgccgtcat cctctttggt ttcggcaatg ccggcgttgc cgtcggtttc agcaacctct
       61 gggaacacgg cggtttcatg cctaacggct ggggcggcat gctggctgct atgtgcgtcg
      121 ttgcggcggc cttccagggc gtcgaactgg taggcattac ggccggggag gctcaaaatc
      181 cgaaacatac tttaaaacga gctgttaata atatcgtttg gcgtattctt attttctata
      241 tcggctcgat tttcgtcatt ctctgtatct atccttggaa cgaagtcagc tacatcggca
      301 gccccttcgt catgaccttc gccaaagtcg gtatttcgtc ggccgcagcc atcatcaact
      361 tcgtcgtcct cacggcagcc ctttccggct gtaacagcgg catgtacagc tcggcccgca
      421 tgatttatac gttagcgcag aacggccagg ctccgaaatg cttcttgaaa ctgtcgaaat
      481 ccggcgttcc ccgcaacggc atcagtgtta ccatcatcgt cttgtttatc ggcgttatct
      541 tgaactacct gattccgaat tcgaagctct tcctctattt atacagcgcc tccgtcttcc
      601 ccggcatggt cgcctggttc gtcctggcct atgcccagaa gaacttccgc aaggcctggg
      661 gcccggaagt catggctaag cacgaattta aatcgcccct ctatccgtat tccaactact
      721 tctgcatcct gttcctggtc ctggtcacca tcggcatgct ctataatccc gacacgcgga
      781 tgtccatctt ctcgtcgtgg gtctacatgg cagccgtcat catcgcctgg ttcgcctgcg
      841 gcctgaacaa gaagaagtac gatcaagacg gtcatctgat caagaacgat gaagaaccgt
      901 aaggcgttgt ctaatggcta acatatcgct tttttatggc gattaaggta taataaagat
      961 gataattcat tctaaaggag atgcatttac atggcagtac aagttatcag cacaaccaag
     1021 gctcccggtg ccgtaggacc ttattctcag gctattaaag ccggtgactt cctctttgcg
     1081 tccggccaga tcgctatcga cccggaaaaa ggcaaagtcg ttgccggaac ggtcgtcgga
     1141 caggctgaac agtgcatgaa gaacgtcggc gctatcttgg aagaagccgg tctcagctac
     1201 gataatgtcg tcaagacgac ggtatatctg acggatatca atttctttgg cgccgtcaat
     1261 gaagtctatg gcaaatattt ccagaagacc ctgccggctc ggtcttgcgt cgaaatcagc
     1321 catcttccca aagacgtctt cgtcgaagtc gaagtcatcg cttactgcgg aaaataaggc
     1381 cttatttctg caccgtcgaa gaaggttctt ttcgcagtgt tgaacggtgt aagtcattta
     1441 aaatagtggt gcattctcat ggcaccacta ttttttttac ggattgtttg gcagaaagcc
     1501 aacaatttta atcgttggat gaatgctgtt tgtagatagt atattttatt ttatctacaa
     1561 acat
//
LOCUS       DAWDXZ010000457         1563 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_13006_length_1563_cov_7.876658, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000457 DAWDXZ010000000
VERSION     DAWDXZ010000457.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13006_length_1563_cov_7.876658"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          335 a          420 c          402 g          406 t
ORIGIN      
        1 atgagcggcg cttcaagcca cttgcgaaga agccttccgc ccatagccgt attggtcttg
       61 tcgaggacgt cgaggagggt gccccggcga ccgccgtcgc ggacgttctg ggtaacttcc
      121 aaatggcgca ggctggcagc gtcgatgacc aggcgccgtt cattatcgat atgttccagc
      181 gaattgatat gcgaaatatc ggactgcatg acatccctga catagttcag gaggatgccg
      241 atgcaggttc cggcccgcga tgcggcggca aaggcttcgg cctcaaaata cgacttggct
      301 tgttccttat aatcgacgct gtcatcgacg gcaaaggccg tcaccgtgca ctgactcagg
      361 tgtcccttca ggtagtgctg taccgttttc atatactcgc ccatattggc gtaaatcagt
      421 tcactcggct cgtagacggc aaggatgtca aataaaacgt cgcccatctc ggaggcagcg
      481 cagtcggccc ataggcactc accggtcgta acatccgtta gggccatgga aatgatgccg
      541 tcacttgccg tgaggcagcc gatatagtta ttctgttttg aagctacggc attttccagg
      601 gtaatcgtcc ccggcgtgat gaccttgatg attttccgct tgacgaggcc cttggccaat
      661 ttgggatctt caatttgttc acaaatcgca accttatacc ctttatggac caagcgctcg
      721 atataggtat cggccgaatg aaaggggacg ccgcacatag ggactttttc cttattgccg
      781 ccggcccggc cggtcaatgt caaatcgagt tcgcgcgaca ccgtcaaggc atcgtcaaaa
      841 aacatctcat agaagtcgcc gaggcggaaa aagaggatct tatcctggca ctgctctttg
      901 acttccatgt actgctccat catgggcgtt aatttttttc ctgccaaagt ctcaccttcc
      961 ttagtgtact aagtggcctt tgagaaccca tgtctgacct ttatcgacag ccacggggac
     1021 ggtatcgccg atggcaacag ttccgtcatt ttcccagatg atcatcttat tgcccgtcgt
     1081 ccgaccaaac caatgatcgg catcgttctt cgtcggacct tcgacgatga cgtcgtagac
     1141 ctgattctcc atttctttat ttttctgcaa gctgtatacg ttttggacat ccatcagacg
     1201 ctgcaggcgg cggctctttt cgtcctgcgg gatttggcct tccatcgtcg ctgccggcgt
     1261 tcccgtccgc ggcgaataga tgaaggtata ggccatatca tactggacgc gttttaaaag
     1321 gtccagcgta tcctggaaca tggcttccgt ttctcccggg aagccgacga tgatatccgt
     1381 cgtcaggacc aaatcgggca tgcgctttct ggcgtagtcg acaagttcta aataatgttc
     1441 cgtcgtatag ccgcggttca tcttctttaa gagttcatcg gacccgcact gaatgggcag
     1501 atgcatgtga ttgacgattt tttggctttc ggcaatggcg tcgatcatgg caaaggtcat
     1561 gtc
//
LOCUS       DAWDXZ010000458         1562 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_13019_length_1562_cov_6.591241, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000458 DAWDXZ010000000
VERSION     DAWDXZ010000458.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13019_length_1562_cov_6.591241"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    914..923
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          367 a          494 c          355 g          336 t
ORIGIN      
        1 ggtcaaaaac agcgcgtcgg cattgcccgt gccctggcca gcgaccccaa gattctcctt
       61 tgcgacgaag cgacgtcggc cctcgacccg cagacgacga aatccatctt ggaactcctc
      121 caggacatca accagcgcct gggcattacc atcgtcatca tcacccatga aatggccgtc
      181 gtccaaaaca tctgccatcg ggcagccgtc atcgaaggcg gcgtcatcaa ggaactgggc
      241 cgcgtcgtcg acatcttcac caatccccag tcccagacca tgaaggactt cgtcacgtcc
      301 gtcatcaaca tggaactgcc gaaggggatc aagaatctcg gcgtctccga ccaaccgacg
      361 ccggaccggg atatgctcgt ccgcctgcgc tttaaaggtc aggctaccca tgaaccggtc
      421 gtctcggaaa tcgtcagcac cttccacgtc aaggtcagcg ttctctacgg caacatcgac
      481 tacatccagg atgaaccttt cggttacttg atcatcgtca tcatgggcga tatggaaacc
      541 caggccaagg ccttctcgta tattaaaaca ttaccgatag gaagtgaggt attaggttat
      601 gtcccaagaa atcattaagt taatgctcca aggcattgaa gaaacgttct atatggtcgc
      661 cgtagccacg gtcatcgccg gcatcatcgg cattcccctg ggcgtcacct tagtcacgac
      721 cagcaagggc cacatcctgc aaaacctctt catcaaccgg attttgggca ccatcgtcaa
      781 catcatccgt tccgtgccct tcatcatcct catggtggcc atcatcccct tgacccgact
      841 cattgccggc acgtccatcg gtacggcagc ggcctgcgtc cccttgacca tcgtcgccat
      901 tcccttcctc agcnnnnnnn nnntcagccg cctcgtcgaa acgtcgatcc gcgatgtcga
      961 ttacggcctc gtcgaagctg ctgaatccat gggcgctacg cctttccaga tcatccgcaa
     1021 ggtcctcctg ccggaagccc tgccgacgat catcaacaac gttacggtcc tcatcgtcaa
     1081 cctcatcggc gcatcggcca tggccggtgc catcggtggc ggcggcctcg gggacatcgc
     1141 catccgctac ggctaccagc gcttccgtcc cgacgtcatg ttagcgacga tcatcatcct
     1201 catcgtcgtc gtcaacctca tccagtacgg cggcgacctg gcatcgcata agaagaataa
     1261 aaaataacag atctgccaaa aaagacatga acagctattt tcggttcatg tcttttttta
     1321 tatcccttgg tcacacgctc cttgcgcaga acgttacatt cagccgcaaa taaatgcttc
     1381 atagccgaag tgaggcatac cctatcgatc aagcttccat cgcttaggca gcgcgtcata
     1441 aaaagacgca ctcaggacac gacaaaaaac gccctgcgaa cgggaatgtc cgcaagacgt
     1501 tgttgtataa gctttcttat gcttctaatt gaccgacgat atcgctgata tgggcgatgc
     1561 cc
//
LOCUS       DAWDXZ010000459         1561 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_13029_length_1561_cov_4.914343, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000459 DAWDXZ010000000
VERSION     DAWDXZ010000459.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13029_length_1561_cov_4.914343"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    522..531
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1053..1062
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          343 a          331 c          401 g          466 t
ORIGIN      
        1 ttccggatgc cgagattatg gctaatgcgg caggtcgatt ttcgtgagga ccgtatcgaa
       61 atgggagagc atatttttga tatgttcatc cttatggaag tcgatggccg ctgcctgcag
      121 cagtttattc aggcggtctg tgatgtatcc gaaagccggt gtcggcaaat tcattttttg
      181 tcgccagagg caatattcgt tggctaagaa gaaaaagtac acgcccatgt aggccttact
      241 cagttgtgtc cgccgtgttt tttcgggaat ggctttgatc aggaaccatt tggcgtataa
      301 ggtgccgaag cggtagaaaa gatcgcgtgg cgaaatggga tatttcgact cccgagccag
      361 tttcttcata tcgatggagc tgtaccgttc gatgtcatag gcatttaata tggtttggaa
      421 acaatgttcg gccttttcga gatagtcgag gtttttggcc ggccgatatt tggcaaggtc
      481 gacctgttcc gggtccttca agaattcgtt gaagatggtc gnnnnnnnnn nccccgtcta
      541 ctgtttgtat cggcgaatag gcattatcgt agaccgaatt ctcgaagagg cgtcccagtg
      601 tatagagtga tttgatgtac acgttataag cggcatccaa atccttcttt ttttcgaggg
      661 cagcgtacag cgttaacacc cgcatcatat cagcgatggc ttgacggcgg acggtgtaaa
      721 aagacaagcc gagggtgtcg atgatggtgc tgcctgaggc agatcttggt gcgaataaga
      781 cttcactcca agctttaccg cggcgatagc gggcctttat ttcggatacg ggcggaagca
      841 gggggagatt aaacagggca tcgtaggctt cgttcatgtt cttaaaacaa tcgaggaggt
      901 tgacgttgtc catctgttcg atttcttttt tattgacgcc aaaataggca atgatgtatt
      961 tatagaagac gtaggccatg gactgcaggg ccataatgtt tttttcgtcg atgcttaaga
     1021 tttgccgata caaggcaatg gcattttgga aannnnnnnn nnttttggaa acagtcattg
     1081 gcttcttgga cgttaaattc ccgtttcttt cccggtactt ttttctttat cacgcgaaga
     1141 ttgctgagct gaccgtaggc gatggccaca tttaataagg tcgggacgtc tttctttttt
     1201 gtcattgctt gaatttccgg cgacaccata cttgtcgtgc agtcggctgc ttttaaaatg
     1261 ccatgccaat tatcctcact gatatgcttt tgaattgcgg cggtatccat gatccgtcac
     1321 cctcctttct aatggttcgc agcgtacatg acggcgtcat attccgtttc gaacaacaga
     1381 ccgatcgatt tctctgtttt taagtcgacg agcacgatat attcccggac gtctcgcgct
     1441 gcctgggcta aggcttcgtc aggcgtcttg tagattttcg ttgacgataa ggcttgttcg
     1501 ggaatccgaa acctcgattc gatccggtat cgttcgttcc atgcttcttt tgctgctgcc
     1561 a
//
LOCUS       DAWDXZ010000460         1560 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_13038_length_1560_cov_4.138206, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000460 DAWDXZ010000000
VERSION     DAWDXZ010000460.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13038_length_1560_cov_4.138206"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1327..1336
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          484 a          363 c          327 g          376 t
ORIGIN      
        1 aaactacctc ctaaatagga ttctccttaa tatcgtactt taataagggg catgattcaa
       61 ttcatttctg ctaaaatatg gtataatgaa aaaaattacc ctcaaggagg cttccatgga
      121 atctactgtt tttacgaatc tgaagggctc agaaggagcc ctgaccttca attttttctg
      181 cgaatccctg attacatcgc tccacacctt gacgcacatc atggaggacg aaggacttac
      241 cgtcccggac aatctcgccg atgtcaccga tgccctcagt gagatgggcg gacacctcat
      301 ggacgattac gctcggggag agctcgattt agaccgcttc aaaaatgaaa tcctcgattt
      361 ttatgacctg aactttgccg tcaatgacgc cttatcggca accatcatga gtcatgacga
      421 tctgcagtat tactactata tttacatgca gggactctat attttcttcc ccaatatgat
      481 ggaagccttt catgccgaca tcgatgacga caacgtcgcc tccgtcttaa accaactcat
      541 tgccgagttc gaacagttat cgagcagcgg ctcctaaaaa cgccatcaaa aaagactgcc
      601 cccgggaaac tttatcggta aagatagagt tccacaggag gcagtctttt tggttaaaag
      661 ataaagaaat aaatgaaaga aatagcggca acgataaacc gatagtaagc aaaagaggcc
      721 agcgtcgacc tattcatgca ggaggttcca gcagcggtac tgatggagca tacgggtaaa
      781 tttttcttta taaataatga ccaccgacag aatggcacct aactggataa aaatctcaaa
      841 cacgctggcc cggggaccgg taaagcctaa catatccccg acgagaatca tatgtcccgt
      901 tgaagatacg ggcaaatact cggtgaagcc ttcaacaatc cccaagataa tggcaataat
      961 agaatcactc attgttttat ctccttttcg ctttaatcgt cctaccaaaa gccttacaat
     1021 actaaaagag tataccacac tagctgacaa aaaaaaaggc tgtccgcgaa ggcaaacagc
     1081 cttttagaca gcccttattc tacatgggat ttatgaaaaa acgattgcga aaaggtaact
     1141 attttttaaa ttacaggatc atcttagtcg tcaagagaaa tgacgtgatt gggattgtcg
     1201 atgacaaact tggcgtactt gggatcacca ttgtgttcga aatacagggt attgccttcc
     1261 agactgccgc gccagctgac gatgatcggg cattcgcggc tcagtttctt cagcattgct
     1321 acaggannnn nnnnnntgaa tttaaggaac cacaagaccg atacataagc tacgataaag
     1381 gccgtaataa agccgatgac aatcatgatc aaatcattgc tgttaagatc gctcaggctc
     1441 ttcaacaggt cgtagaaaca ggccacgatc atgaccggta cagcaataat gaaggaaaag
     1501 tcggccgctg ccttacgact gaggcctaag aacagaccgc cggcgatggt cgatccggac
//
LOCUS       DAWDXZ010000461         1557 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_13060_length_1557_cov_5.061252, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000461 DAWDXZ010000000
VERSION     DAWDXZ010000461.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13060_length_1557_cov_5.061252"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          342 a          388 c          378 g          449 t
ORIGIN      
        1 cataaaaaat ttgagaaggg agttgtaatt atggctcgaa gaatgaggcg atcccaggat
       61 agacaggtat tccgccatac cgccgtcaat tccaagcgta tcaatgttaa ccctaaaatt
      121 tatcgtggag gtatcaggat gtgaatgata agcgtgctac cgtctgtgtc cggctgaatc
      181 tcgatgccga ctgttacgag ctggccgcca aagtttggcg ctctctcgat atggagctcg
      241 atttttatga gttcctcgcc ctggaactgc aaatccggct cattgagcta tctggttttt
      301 tggaggatga taaaaatgat gcttaacgtt tattccattc gtgacgttcg ttcgggcttt
      361 ctcactccta ctgtcgatca gaatgactat atcgcggctc gcaactttgc gaatgccatt
      421 atggaatccc acggcgttct ttttacccat gcttccgatt ttcagctttt ccgtattggt
      481 gagtttgata ctgataaggg cgttcttgtt ccggccccgg tgcatgagct cgtttccgac
      541 ggcgcggaag tccttcgtgc tatgaaaaag gaggaagcca atgtttgaga cttggcaccg
      601 cgaacagcag catttttgtt ccgagcctgg ttgcggcgag aagattcttt actcccccga
      661 gtttgatcgc tttggcgtta tgaccctcaa ggagagcggt aaagaggacc tctatgcctt
      721 tatccagagt cacaaagatt ccgttgatct ccataaaatc atggacaggt ttaacgctgg
      781 tgatactgcc gccctgcaaa aggtgcaagg tatgtttggt gatttctccc agatgcctca
      841 gacctatgct gggttgctca atcacatgat agaggccgag cagacgttta tgagcttgcc
      901 cctcgaaact cgcgagaagt tcggccaatc tttccatgcc tggcttgctc aggcaggctc
      961 tgccgccctg gccgtgtgaa caacagagaa cgaggggccc cctgtgggcc cctccttttg
     1021 aacaaccgtt acagactgga ggtattaaat tgtctcgcaa tgaaaataca agatttgctc
     1081 ttaatcctac taaccttgat attgctcgta gcacttttcg gcgtgaccat agtgttaaac
     1141 tcagtttcaa cgtcggagac gttatcccct tctatgtgga tgaggttctt cccggtgata
     1201 ctttccagct aaagacttcc atggtggccc ggctccaaac tttgctcact cccatgatgg
     1261 acaatcttta tctcgacact tatttttatt ttgtgcccaa tcgtataggc tggcagcatt
     1321 ggcgtgagct tatgggcgag aacacgcagt ccgcgtggat tcctacgacc gagtattccg
     1381 ttcctcaggt cacggccccc tctggtggtt ggtctattgg ctctattgcc gattacatgg
     1441 gcattcctac tggtgtcgcc aatctttctg tcaacgcact tcctttccgg gcgtatgcgc
     1501 tcattatgaa cgagtggttt cgtgacgaga atctttctga tccgctcaac atccctg
//
LOCUS       DAWDXZ010000462         1551 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_13105_length_1551_cov_6.310829, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000462 DAWDXZ010000000
VERSION     DAWDXZ010000462.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13105_length_1551_cov_6.310829"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          422 a          339 c          383 g          407 t
ORIGIN      
        1 tcttaggtac gcgagaatgt cagggctatg tagagcccgt tgactatggt gaagctcccg
       61 gtgtttatga tgatactccg gctattccct tgggaacgat gttccatggc tttaactatc
      121 ctgatgaaac ggggcgagac atgatgcagg tccgtttttg gcagcctgtc atgaatcacg
      181 gcgtcattcg ctttattcgc ccggaagcct gcaccctcgt gcggaatatt cacaaggtga
      241 aagccaaagt ctttacaccg gacgatatct gctccgtgga cgagaagcgg cggtgctgga
      301 aggagacttg ccatgagctg gattatggag ttagttaaaa cctacgatgc ccatgaagac
      361 ttggtgggaa aagagaatat agaggggagt aaggctgttt taatgccaat ctgtcatgtc
      421 gtacagaaag cccaaatcga agtgaccatc gacggtgagg gtaactttag tcatgccagc
      481 gtccttccca aagcagaaca gtcaaccctc cttccctgta ccccggcttc ggaagtgagg
      541 acgtcgaact tggccccaca tccgctgcat gacaaattaa tttatattgc ccgtgacggt
      601 gacttatatc gagaagaacg ggataaaaaa ggacgaagcc cttactcact ctatgtagag
      661 ctcttaggca agtgggtcca ttctccttat agtatcccta aggtagaagc ggtgtataaa
      721 tacgttacgc agcatgatat catcgatgac ctgtgcaggg ccaatccgcc cgtcctctac
      781 cgtgatgata agggcaacat catgcaaaaa tggccttata aagaccgacc taaaccggat
      841 atcttttctg cggtaagtga cgacatttta aaagccttag ttcggtttcg agtcatccat
      901 atccccggtg ataatgaacc tgatttatgg aaggatcagg acttgcagcg gcaatatata
      961 gcctttttta agcatgaatt ttctaaagat ttggagcttt gttacgcaac gggaaaaatg
     1021 atgcgtcctg cgggcaaaca aggcaagaga atccgttcgc caaaggacaa gtctaagctc
     1081 atttcatcca atgatactaa gggacttacc tttcgcggtc gttttaaaaa cgcgcatgaa
     1141 tgtttgtctc tcggccatga aaccttgcaa aaagctacca atgccttggc ctggcttatt
     1201 cgcaaccaag gtcatgattg tgagggccgc gtctttttgg cttggagtag ttcaacgccg
     1261 cttcccgata ttttgtcggc tacgaacgat gttattggtg atgaatggag tactccttta
     1321 gaaattacac agccgcagac attaacgcaa tgggtagaaa gctttgaccg tgctattaag
     1381 ggatattacc ataattttga aaaagatccg aatgcgaaag cgaacgttat cgttctcgat
     1441 gcgattacat cagggcgact atccatttgt tattacgatg agttttcggg gagggatttt
     1501 attcggcgta ttgaaaaatg gcataaatcg ggccgctgga ttcagcataa a
//
LOCUS       DAWDXZ010000463         1543 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_13164_length_1543_cov_5.723118, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000463 DAWDXZ010000000
VERSION     DAWDXZ010000463.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13164_length_1543_cov_5.723118"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1255..1264
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          338 a          395 c          430 g          370 t
ORIGIN      
        1 aactggccgt gtccatcgga caggtcgtca gcaatgacat cctcgtcgac gaccgttttg
       61 atatgaccga gcagctgctc catgctcaaa agacgaacga ccaggtccgc tacattctgg
      121 tgacctatcc ggacggacgt attctggcca gtacttttac ggacggactg ccgaagggcc
      181 ttccggaacg gcgggatatg actatggaag gcgtcgggca ggcagtgact tttgacagca
      241 ccgaaggcta tatccgagaa attctcgttc ccatcgacgg cggtttagtc ggatattttc
      301 gagtcggtct gtccgagcgg cccatgatgc agctcatgtg gaagcgcttt gtcgagacga
      361 tgctgattat cctggccatc tgtctggtgg cctctgcttt ggctacgcga tatgccaggg
      421 cttttctgct ccccgttcaa gctctggcga aagccattcg tcaaatcggc cgcgggaatt
      481 acaacgttcg cgtttccgtc gaaacgaaag acgatgtcgg ccagttggct aaggcgttca
      541 atgtcatgag caaccgtctg tcggtcaagg atcgggaaaa ctcgaaactt ttgacggccc
      601 ttcgggaaaa ggagaagaat agagtttggc tcatcgatca gctgttttcg gctcgtgaag
      661 atgaacggcg ccgtatttca cgagagctcc acgatgaaac gagtcagtct atggtatcga
      721 tcttagccta cctgcggctc attcaggacc ggactgaaag tcccgacgtg cgggaattgg
      781 tgaccggtgt ccgtgaattg acgagagaga ctctggaaag cctgcgccat ttggcggtca
      841 acctccatcc gccgctcctc gacgatttag gcctcatcgt agccattgaa aaatatctcg
      901 atacgtatcg gcagacccag ccgaatcttt ccgtgtcttt ctgtcatgac ggcgatattg
      961 ctaaagtgtc gcggccagtt gctttatttt gttaccgcat ggtgcaggaa gggctgacca
     1021 acatcgctcg ccatgcccaa gctcatacag tgacgattga actgaacatt ctgccgtcgt
     1081 ccttgtcttt acgtattgcc gatgacggcg tcggtttcag tgccgcccgg gctgagcagg
     1141 cacggcttga taatcattta gggctggtca gcatgcagga acggactgac gtgttgaatg
     1201 gcacttttta tatcgacggc cgaccgggga agggaacgac cctgaccata atgcnnnnnn
     1261 nnnnttacca tgtacagcga gcaggagtac gtgactgaat tgatgcagct cggtgctgac
     1321 gggtacttgt gcaaagatac ccttgatgca gagctcttta aaggccttcg tgccgtcgct
     1381 tccggcgagc gttatctaag tgaacgcgaa gcccggagtc tccttaacgg tgtcttagca
     1441 gagaaaaaag atatgccgac cttgtcggaa cgggagcagg aagtcctcgt tgccttagta
     1501 cacggccatt cgctgtcgtc tatcggggat acgctgcacc ttt
//
LOCUS       DAWDXZ010000464         1540 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_13202_length_1540_cov_6.185185, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000464 DAWDXZ010000000
VERSION     DAWDXZ010000464.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13202_length_1540_cov_6.185185"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          383 a          405 c          371 g          381 t
ORIGIN      
        1 gtgtcattct gtacgaactg tgcgatcgta atcgggaaca tcagtacaga cgatgcaaag
       61 atgatgggga taacgccggc ctggttgact ttcagcggaa tgtgtgtcga gtggccgcca
      121 tacatcttac gaccgacaat gcgttttgca tattgtaccg caacgcgccg ctgtccctgc
      181 gtaacttcga tgaccacaat gatcatgaac aaagcgataa cgacaaaaag taaggcctgg
      241 aagatattaa tcgaaccggc ctgaatatac tgataaatcg tcatgacgcc ttgggggaaa
      301 cgcgctacga taccggcgaa gatgatcagc gaaatgccgt tgccgatgcc gccggctgtg
      361 atctggtcac cgagccacat gaggaaacaa gtccctgccg taagaatcag tgataccatg
      421 acgaccatag cccagctgtc gacgatgagc gcaccgttga ctttcagtgc atagctcatg
      481 gcaaaggcct gaatcaatgc caggacaacc gtaccgtacc gcgtgacctt ttggatctta
      541 tcgcggcctt cttgattatc cttggcccat tgttcaaacg ttggaacgac ggcggtcagc
      601 agctgcatga taatcgacgc gttgatgtac ggcgtgatgc tcatggccag gatggagaac
      661 ttactcagtg ccccaccggc aaacaagtcg agtaatccga acaagttgcc gctgctgaag
      721 aggttttcaa tgaccgacga gtcgacaccc ggtacgggga tgtgtacacc cagtctgaaa
      781 acgacgaaca taataagagt gaagaccgcc cgtttccgga attccggaac gttaagtacg
      841 ttttggatgt tctctaacat cgcaaatcac ccctactgtt ctgctaccgg ttcttctccc
      901 ggtactacaa ccttaccacc tgcagcttca atcttttctt tagcagattt cgtgaaacca
      961 ttggctacga cggtcagttt cttttcgagt tcgccattgc cgaggatgcg gatgccgtcg
     1021 cggacattct taacgatgcc agcttcgact aaagcaacgg gatcgacagt cgtgccgtct
     1081 tcaaagcggt tgagtacaga aacattaact tctgcatatt ctttacccca aatgttggtg
     1141 aaaccacgtt tcgggagcaa acgatacagc ggcatctggc caccttcgaa gcccgggcgc
     1201 ttaccgccac cgctacgaga aagctgacct ttcatacctt taccggccgt tttgccgtta
     1261 ccggaaccga taccacggcc gacgcggtta cgttttttct tggaaccagg aacaatactc
     1321 atttcatgaa gtttcaattc agcgcacctc cttctatatt aaatttcttc tacttttacc
     1381 aagtgttcta ctttatgaag catccctttg atctgggggg ttacttcttt tactacgctg
     1441 gagttcgtct tgtggagacc gagggcaacg atggttgcgc gctgatcgcc aggacggcca
     1501 gccaaactct ttacgagtgt aactttaacc tgagccatct
//
LOCUS       DAWDXZ010000465         1539 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_13205_length_1539_cov_9.484501, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000465 DAWDXZ010000000
VERSION     DAWDXZ010000465.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13205_length_1539_cov_9.484501"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          363 a          383 c          445 g          348 t
ORIGIN      
        1 ctgccgctga cgctgaattt ctctgatttc aacggcactg agaaaatcgc ctatcctccc
       61 aatgccttgc cgacgaaggg ggaaccggac ggcacggatc ccgaggcagg cgacctgtgc
      121 tactatgagc cttggggcaa tttgtgcatc ttctaccagg attttcgcta ttctaaatcc
      181 ctcattaaac tggggcgggt aacctccggg atggaagtgc tgtccggcat gaaaggcgat
      241 tttacggcgt ccttagaaag ggtgaactga tatggaatat acgacattag gctcgacgga
      301 tattgaagtt tccaaaattt gcgtaggctg tatgagtttc ggcaaggccg gtacgatgca
      361 cgactggacg ctggacgaag cggcgaccga ggccatggtg aagcacgccc ttacgctagg
      421 catcaatttt tttgatacgg ccaactgtta ttctgccgga acgagtgaag aatacgtagg
      481 ccgggcgctg aaaaagaacg tctccaggga caaggtggtc atcgcctcca aggtctattt
      541 caacgacggc cgcctgtccc gtaaggcgat ttttcgcgag attgacggca ctttgaagcg
      601 cctcggaacg gactatctcg acctctatat catccaccgc ttcgactatg aaacgcctat
      661 tgaagaaacg atggaagccc tgaatgagtt ggtgcggtcc ggtaaggtgc gggctctggg
      721 ggcatcggcc atgtatggct atcagtttta caatatgcag ctctgcgccc gcgatcatgg
      781 ctgggctcgt ttcgaggcca tggagaacca ctacagcctc ctgtaccgcg aggatgaacg
      841 cgagttgatt cccctttgta agcaaatggg cgtgagccta atgccttaca gtcccttggc
      901 agcgggacac ctggctcgac ctacgtggaa gtcggacacc ctccgctccc ggacggaccg
      961 ggtcgccatg ggaaagtatg accgtacgga agaacaggat atggctattg tccgccgtgt
     1021 ccacgaattg gccgaaaagc acggcgtaaa gatgtcggaa atggccctgg cttggcagtg
     1081 ggccaagggc gtggcctcac ctatcgtcgg tgcgacgaag ccccaatatc tggatgatgc
     1141 cgcagcggcc ctgaccgtca aactcaccga cgaagaggcg gcatacctgg aagcgcctta
     1201 tgtgccgcac gccgttgtcg gtgccgtcga tcacaatccg ccccaaggcg tcatgctgct
     1261 cgatgaaaag aaataaggcg atgtcgtttc agaagaagcc ctaccgattt cggggagatg
     1321 ctttcttttg tttttgatat ttatttcgaa agagacgccg ttccatgagg agcggctttt
     1381 ttctattgcc gtcgatgacc ccgatgaagg gcagaagctg tggataatgg ccgggtcgac
     1441 aaatgagatt gaaactgata caatgaaaca taataatgac ggttagtttt agtgaagacc
     1501 taggctttaa acggtgggag gtggatttgg ccatggcga
//
LOCUS       DAWDXZ010000466         1536 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_13231_length_1536_cov_7.608373, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000466 DAWDXZ010000000
VERSION     DAWDXZ010000466.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13231_length_1536_cov_7.608373"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          363 a          467 c          374 g          332 t
ORIGIN      
        1 ccgagggcgg tctctatgtg cccgaagcct ttcccgagga tgtcctttct tataaagaac
       61 tgagcggcaa gtcctatcag gagattgccg acgtcgtctt atctcatttc ttcaccaact
      121 ttacggccga tgaacgaaag gctatgatcg cctcggctta caatgaagaa actttccgcg
      181 atgatcgcat cgtgcccctt cattccatcg ataccgacct gtcggtggct gaattgttcc
      241 acggccggac cctggccttt aaggacctgg ccctgtccct ctttccgtac cttctgacgg
      301 ctgccaagaa gcaggaacag gaaaaacggg agatcctcat tttgacggcg accagcggcg
      361 atacgggcaa agctgccctt gaaggcttca aagacgttcc cggcaccaac atcatggtct
      421 tttaccccag tgaaggcgtc agccccatgc agcagcagca gatgcagaag caggaagggg
      481 acaacgtccg cgtatcggcc attcacggta acttcgacga cgcccagagc ttcttaaagc
      541 gcgtcttcac caaccccgac gttaatgctt atgccaacga aaagggcgtc cttttctcca
      601 gtgctaattc gatcaacatc ggccgtctct tcccgcagat cgtctactac gtatcgacgt
      661 atatcaccct cgtcgacgac ggcgccatcg gcgaagggga aaccttcgac gtcatcgtgc
      721 cgacggggaa cttcggcaac atcctggccg gttatttcgc caagaaaatg ggcattccca
      781 tcggcaaatt gatctgcgcc tccaataaga ataaggtcct ggccgacttc tttgaaacgg
      841 gcgtctacga tatgaaccgc gacttctaca cgacggacag cccgtccatg gacatcctct
      901 tgtcgagcaa cttcgaacgc ttcctcttct atgccgccgg tgaagatgcc caaaaggtcg
      961 cccagtggga aaaagaactc ttagaaacgg gaaccatgcg ggttacgccg gaagaactcc
     1021 aggccctccg tcaggacttt gaaggcggtt ggatcggaga tgaagaaacg gaagccgtca
     1081 tcaaccacgt ctacaacgac ttcggctacc tcctcgaccc ccatacggcc gttgcctacg
     1141 gtgtcggcat gaaccgtcac cgcctgggcc tcgacgaagg ccgtcacacc gtcgtcatgg
     1201 ccacggctca tccttataaa ttcccgccgg tcatcagtgc cgccctggca cttcgggaaa
     1261 gcgccgatcc cttcgatacc ctcgtcgata tcagcagcgt caccgggatt gccctgccga
     1321 aacccttggc cgacctcaag cataaaccgg tacgcttcaa agattccatc gataaggaag
     1381 ccatgaaaca ggctgtctta tccttcgtcg acgatatttc gtccaaggcc tagaaagaag
     1441 ggcagactgt tgaataaagg tgtactcttc gccatcctgt cggctctggc cttcagtatc
     1501 cagaatatca tcgtaaaagt cctatcctat acggtc
//
LOCUS       DAWDXZ010000467         1534 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_13251_length_1534_cov_7.513185, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000467 DAWDXZ010000000
VERSION     DAWDXZ010000467.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13251_length_1534_cov_7.513185"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    534..543
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          377 a          491 c          380 g          276 t
ORIGIN      
        1 atctccctct ttgaagccct gtcgtcgccc gacgccatcc ccgtcaccaa acgggcccag
       61 acgtcccttg aaaactttgc cgccatgatc tttgatctcc tcaatgagat cgacggcctc
      121 gacgtcttga gcctcatcga aaaggtcatc aaagacacgg gctacggcga catgctcgat
      181 aaagaagccg aacacgatcc ccaaggcgta agccgcaagg aaaacgtcgg cgaattcctg
      241 tccgtcgcta aagactacat ggacagcaac cccgacggca acctgcagga cttcctcgaa
      301 aatgtagccc tggtcagcga cgtcgacgac tttgaaagca gcgaatcgaa ggtcaccctc
      361 atgaccctcc acgccgcgaa aggccttgaa tttcctgtcg tctttttggc cggcctcgac
      421 gaaggtctgt tcccgcacag taggaccctg atggatcccg aacagattga agaagaacgg
      481 cgcctggcct acgtcggcat cacccgtgcc gaacggcagc tctacgtcac caannnnnnn
      541 nnntacaagc gaaagagcgc catgccccag agccggatga cgtccgttcc cggtcagcag
      601 cgggtgtcga tcctgtcgaa gcccgtagcc acgagccttc ccaagaagca cgccgtgacc
      661 gactcttttg ccaagggcga taaagtgcgc cataagattt ggggcatcgg caccgtcctc
      721 gacgtcatcg gcgacgggcc gaacatgcag atgaaaattc aatttccgac gaaaggcgtc
      781 cgccaagtcg tcgttaaatt cgcaccgtta gaaaaagtat agccatttcg atacgcgtaa
      841 aggaagggaa tcttatggaa caagaaaaag cgcggctcct ggccctgcga caaaaaatcg
      901 atgaactgag ctaccagtat tacaccctcg acaagccgac catgagtgac tacgaatacg
      961 acatgctcta ccgggagctg gaaaacatcg aaaaagccca tcccgaatgg gtgacgcccg
     1021 attcgccgac ccagcgcgtc ggctctaaga tttccggcgg ctttgaaaaa tatacccacg
     1081 accggcccat gctgagcctg ggcgacgtct tcaacgacaa tgaacttcgg gaattcgatc
     1141 gccgcgtccg cagcgacctc ggcggggaag ccttagaata cgtcgtcgaa ctcaaaatcg
     1201 atggccttgc cgtcaacctc atttatgaaa acggtcagtt cgtccgcggc gtcacccgcg
     1261 gcgacggcgt cgtcggtgaa gacatcacca acaacgtccg caccatccgc accatacccc
     1321 ttcgcatcga cagcacgtcg tcccacatcg aaatccgcgg cgaagtttac atgcccgtcg
     1381 cctctttttc ggccctcaac gagcagcggc gaaacgacga actcgaaccc tttgccaatc
     1441 cccgtaacgc cgctgccggc tccctgcgcc agctcgatcc tcagatcacg gcccagcgca
     1501 aactggcctt cttcgcctac gccttagggg gcaa
//
LOCUS       DAWDXZ010000468         1532 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_13263_length_1532_cov_7.486798, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000468 DAWDXZ010000000
VERSION     DAWDXZ010000468.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13263_length_1532_cov_7.486798"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          360 a          393 c          351 g          428 t
ORIGIN      
        1 atacgccttg gcataggccg ccgccgtatc tgcagccccc agtcccgact gctcgaattt
       61 caaatcactg ccgtcgtaaa tccgtacgcc aatgacgatg ccggctcgaa ggtaaacgtc
      121 catatggtga ctgccgaaga taaggcgtga cgtatcctga tcagggacag tgctgtcagc
      181 ctgtccgtaa gcaccttcca agtcggattg ggtcatcccc aagtaaacgc cgccaatgga
      241 ttcatagtga tcgccatgga tagatcgccg caggggcgtc gtgttgtcca tgatgaggta
      301 ttggtgagac ttatgaccca tcaagtccag gctcatgacc cgatccccgg cctgttcacg
      361 aatggtaaac ttccctttac gaggatagcc gtcggtgtag tcttcgatat cagtaacggc
      421 agatccgttg atggcggcgc cttcgacagt cagtactaaa tcgcccttgg tattatacca
      481 gtctccggca gccggtgcca gccaatcggc gacagtctga ttcggctgag cctgggttgt
      541 cgaagcgacg gtcacctgcg gttcggccgt tggaacagcc gcctcttcgg cagcacaaac
      601 ggctgataaa ctcatcgtca tcagcgcaca cagaaccatg ttttttgcta tttttttcat
      661 gctaaatcgc tccctttgtt gacttcttgc agccccttat tatacgcttc ttcctagagg
      721 aaaacaatag ttcccacgcc tgtttattct tgtttacgaa acacctccat tcccggcgtt
      781 caaacttcgt aaacaacggc aaggatgttt acaacatccc tccattaaaa ttatacttaa
      841 ttggtcagct tttcattgac ataaaggggc tcatctttta taataaataa attgcagtct
      901 atcttgtagt caggaacgaa agaggagcaa ttaatgaata tactggattt gatcttaata
      961 gccatcggct tgtcgatgga tgcttttgct gtatccatct gtaagggctt atcccttcgc
     1021 aacgtcaaat ggcatcatat gattattgtc ggtttatggt ttggtggatt tcaggcattg
     1081 atgccctcca tcggctatat tttgggcagc ttttttattg aattaatcga cgcctacgac
     1141 cattgggtta ccttcattct cctgagcgca atcggtctta atatgattaa agagtctcgt
     1201 gaagcgagtg gcagctgcaa cccgtccctc caaccctata ctatgctgat gctggccatt
     1261 gcaacgagta tcgatgcctt agccgtcggc gtcaccttcg ccttcatgaa tatacgggtg
     1321 attccctcgg ttacaatcat cggggtcacg acctttctgc tctctgccat tggcataagg
     1381 gccggcggat tatttgggaa ccactataag agcaaagccg aacttgtcgg cggcatcgtt
     1441 ctcatcctta ttgggttaaa gattctcctc caaggattag gcattctata aaggcggtat
     1501 ttttacaggc cagatgtaaa aatgctcccc tc
//
LOCUS       DAWDXZ010000469         1530 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_13279_length_1530_cov_11.294237, whole genome shotgun
            sequence.
ACCESSION   DAWDXZ010000469 DAWDXZ010000000
VERSION     DAWDXZ010000469.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13279_length_1530_cov_11.294237"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1155..1164
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          399 a          430 c          381 g          310 t
ORIGIN      
        1 cgtaaacgtc agccgcaacc gccgtttacg acgtcgacga tgcagcagga atgcgtcggc
       61 aagctcaatt tcggcgccaa gaagacgatg atgctggccc agcagctcta tgaaggcctc
      121 gatatgggcg accagggcca cgtcggcctc atcacctaca tgcgtaccga ctcggtccgc
      181 atcaacgacg acatggtcca ggcggcgcgg gactttatta cggctaacta cggtgctgaa
      241 tacgtccctg aaaagccgcg ggtctataag accaagcagt cgagtcagga tgcccacgaa
      301 gccattcgcc cgacgtcttt ggaattgacg ccctataagg tttccgactt cctgtcgcgg
      361 gaccaattgc gtctttatac cttgatttgg aatcgctttt tggccagcca aatggaatcg
      421 gtcgaaacgg aacacatggc catcgtcatt gaaagcggca agtacgaact gcgggccgcc
      481 ggctataagg tcctcttcaa gggctttacg gagctctatg aagatgccaa gaaggataag
      541 accttgaccg aactgcccca tatttcggcc aataccatcg tccataacaa ggccgttacg
      601 ccccagcagc actttacgca gccgccggca cgctacaccg aagccagcct gatcaagacc
      661 ctcgaagaaa agggcatcgg ccggccgagt acctacgcgc ccatcatgga cacgatccaa
      721 aaccgcaact acgtcgaaaa gaaggacaag cagttcattc cgacggaact cggcgtcatc
      781 atcgtcgacc tcctcaagaa atactttgcc cgcatcatca acgtcagctt tacggcccat
      841 atggaagaat ccctcgacgc catcgaacaa ggcgacgata cgtatcccca cgtccttcag
      901 gaattttatg acatctttaa gcctgaaatg gacgaggccg aaacgaagat ggaaaaggtc
      961 accatttccg ggcaggaatc gggccagatc tgcgaagtct gcgggtcgcc catggtctat
     1021 aaattcggcc gcttcggcaa attcctggcc tgctccaact tcccggaatg ccgcaacacc
     1081 aaggccatcg tcgaagattt gggcattacc tgcccgaagt gcggcaaggg gacgctcatc
     1141 aagcgcaagt cgaannnnnn nnnnccggac tgcgacttcg tcctctggaa ccagcctgtc
     1201 gacaagaaat gcgacttgtg cggcagcatc atgattgtca aacattataa aaaaggccct
     1261 gacaagctct tttgcagcaa tggggactgt gaaaaccata aaaaaggtga ggtagttagt
     1321 caaccactca cagaagagtg agcttgtaag tcagaaaagc ataggtagtt gcgacatcct
     1381 aaagatgtct ccctaattct gcctacatca tcgggcagtt gaccatgccc gttttgccgg
     1441 ggagatttac tccgaggcag ttgttatcct ctcgtaacga gggtgattgt ctccggaaat
     1501 ccacctcgtc ccaagatgct ggatattcat
//
LOCUS       DAWDXZ010000470         1526 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_13325_length_1526_cov_6.312033, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000470 DAWDXZ010000000
VERSION     DAWDXZ010000470.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13325_length_1526_cov_6.312033"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    607..616
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          439 a          397 c          410 g          270 t
ORIGIN      
        1 ccagttggtc cagtcaatat ccgtcgacat accggcggtg aacatcaaag cagcagcttc
       61 accgaaacca cggccggcag ccatgataag gccagtcatg atacggggga agcaggccgg
      121 caagagaacg tgccaaatgg tctgccaatg ggaggcgccg aggcccaaac tgccgttgcg
      181 gtagctcggc ggcaagttgc gcagagcatc ttctgtaacc gacgtaacca agggcaaggt
      241 cagaatggat acggccaaag caccggccat gaggttccac tgggaaccgg tcatgacgat
      301 gaagaccaag taaccgaaca agccgacgac gatagacggc agggatgcca aggtttcaac
      361 agccatgcgg acccaatggg taatgcgtcc ttttttcgcg tattcagcca ggaagatacc
      421 tgcaggaata ccgataatcg cactggcaac caaggctaat acgactaaat agatcgtatt
      481 aaagaacatg tttcctaagc cggcaccggt aaaggataag agttgcggtg tcatgccgct
      541 tacgccgctg acgacgacgt acagaacgaa ggcaatcagc aatacgacag caaaggctgc
      601 gccacannnn nnnnnntttt ttggtttcaa tacttgtcat attaactccg tcctcctttt
      661 aattcggaag cggcattgat ctggtggata ataaaaatga acaggaacga caacaggaac
      721 aagagcaagg ccatcgtcca caatgcggca ttgtattcgc cgccttccat cgcgcccccc
      781 atatccgcag agattgccgt cgtaagcgtg ctggccggca ggaacaagga cgtcgggaag
      841 actttcatct gaccgataac catagcaaca gccaaggctt cgcccaaggc acgggccagg
      901 cccaggataa tgccggtgaa aataccgctc ttagccgcag gaagtacgac gtgagcaatc
      961 gtttcccagc gagtcgcgcc gaggccgtaa gaagcttcgc gccacgattt gggaacagcc
     1021 gccatcgcgt cggcagacat cgtcgtaatc gtcgggaaaa tcatgacggc caagacgata
     1081 ccggcagcta agacagaaaa gccaaacggc atggggaata tatggcgcag ggccggaatg
     1141 agcactgtca tgccgacaaa gccataaaca acagaaggaa tgcccgtaaa taattcgata
     1201 gccggctgaa tcaggcggcg ccgctggggc gtcgcgattt cagtcatata aattgacgag
     1261 gccaggctga agggcaagga aataagtaac gccaacagac acgtaaccag tgaacctgcc
     1321 aagaacatag cagccccgat ttgaccgccg ccttcggccg tatcgctcgg tttccattga
     1381 ccggaaaaga gaaattcagt tacgctgtgg ccaaaagtga aaaacgtatc tgtaccttta
     1441 acaaagagaa aagcgccaat gagaagcggc accagtgcca taccgatgcc gcaaatggtg
     1501 atgaacgttt tggctgcttt ctcgtt
//
LOCUS       DAWDXZ010000471         1522 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_13345_length_1522_cov_8.711656, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000471 DAWDXZ010000000
VERSION     DAWDXZ010000471.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13345_length_1522_cov_8.711656"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          319 a          394 c          397 g          412 t
ORIGIN      
        1 ggtctgaatg tcgccttctt ctttgaattc gtcgagggcc gggaggatgt cgatgagcgt
       61 cgtgccgccg cccgatacga taccttcttc aacagctgcg cgagtagcgt tgagggcatc
      121 ttcgagacga tatttcttat ctttcaattc gacttccgta gcagcgccga cttcgatgac
      181 agcgacaccg ccggacatct tagccaaacg ttcctggagt ttttctttat cgaaatcaga
      241 cgtcgtgtca gcgatctgag atttgatctg agctacgcgt tctttgatgg cctgagcatc
      301 gccgtggcct tcaacgatcg tcgtttcatc tttcgttacg cggacctggc gagccgtgcc
      361 gaggtcttcc atggtaacgc tgtcgagttt acggccgaca tcttctgtga tgaccgtagc
      421 acccgtaagg gttgcaatat cctgaagcat cgctttgcgg cggtcgccga agcccggagc
      481 tttaacagca acggctttga aggtgccgcg gaggcggttg acgaccaagg tagcgagagc
      541 ttcgccttcg acgtcttcag cgatgatgag cagttctttg ccggactgaa cgactttttc
      601 gagaaccggg agcatttcct ggatcgaagc gatcttgcgg tccgtaatga ggatatacgg
      661 ttcgctcatg acagcttcca tcttatccgg atcggatacc atgtacgggg agatgtagcc
      721 tcggtcgaac tgcatgcctt cgacgacgga gagctgcgtg cccatggtct tcgattcttc
      781 aacggtgatg acgccgtcat tgccgacttt ttccatagcg tcagcgatga ggccgccgac
      841 ttcttcgtca tctgcagaga tagaagctac ctgagcgatg gaagctttgt cggaaacggg
      901 gatcgaacgt tttttgattt cttcgacgag tttcgccgta gctttttcaa tgcctcgttt
      961 gaggatcatc ggattggcac cggcagcgac gttgcgcatg ccttcttcga tcatagcctg
     1021 agccaggatc gtagccgtcg tcgtgccgtc accggctacg tcgttggtct tggtagcgac
     1081 ttctttgacg agctgagcgc ccatattttc aaagggatct tccaattcga tatcgcgggc
     1141 gatggtaaca ccatcgttgg tgatcgtcgg agccccgaat tttttatcga gaacgacgtt
     1201 gcggccttta ggccccaagg taacttttac ggcatttgcc aaagcgtcga cgcctttgcc
     1261 gagagcacga cgtgcatctt cattgaacaa aatctgtttt gccatcttta acacactcct
     1321 atatgaaaag tcagtagttt taatttatta gataatagcc agaatgtcgc gttcgctgac
     1381 gattaaataa tcggtgcctt cgaatttgac ttccgtaccg gcatatttcg agaaaatgat
     1441 tttatcgcca actttaacat ccaatgcaag gcgtttcccg tcatccagaa gcttgcccga
     1501 accgacggcg atgacttcac cc
//
LOCUS       DAWDXZ010000472         1521 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_13350_length_1521_cov_10.813779, whole genome shotgun
            sequence.
ACCESSION   DAWDXZ010000472 DAWDXZ010000000
VERSION     DAWDXZ010000472.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13350_length_1521_cov_10.813779"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          412 a          357 c          378 g          374 t
ORIGIN      
        1 ggactatttt ttcaaaaaaa agctatccca aacaaaaggg atagctttac ggtacatcgg
       61 cactcactca tcgtcttttt gaatataaac gactcgctgc ggatacggaa tatcaatgcc
      121 gttggcatca aaggtgtcct tgaccttgga catggtatcg tagaatacag gcaggtaatc
      181 ggccttggca accttcggat aggctgcaat tcgcaccgag ttgtcaccga attccttgat
      241 tccgatttct aaatcgtcgg cgttgaggat acgaggttcc tctttaaaga tctgtttcaa
      301 taaggcgatt gccttatggt gatcattgtt atagccaata tcaaataaga aggtaatata
      361 gcgctccgtt tgatatgagc tattggaaac ttgagacgtc gtaatcgtcg aattcgggat
      421 aaaaacggtc ttgttgtcct tggtggtaat ggccgtattc agcatttgaa tgcccttgac
      481 actgccttct acaccgccga cagcgatcca gtcaccggct ttaaagggct taaagactaa
      541 aatcaacagg cctgacgcaa agtttgacag gttgccctgc aaagctaagc cgacagccaa
      601 gccgaaggca ccgaaggcgg ccaagaagga attcgtagga atccccagct tgttgagagc
      661 cgacaagagg acaatggcca tgagggcata gtaaataatt tgactgacaa aggtaatgac
      721 tgtatggtca taattggcat gggtcatcat gcgaatggcc agttttttta tagctccggc
      781 gatatagcga ccgatgatga agatgatgat ggccgcaatg atgtccggcg cataatccac
      841 caggaaatca ttgaattggc caagggccga aacgccttcc gtaacttggt tcttaacgtc
      901 agcccggacg gtgctggcag cctggaccgt attggcagca agattcatag acagtacctc
      961 ctgtgtacgc acatacagaa aaagcaaatt ataaaactat gtgccattat tatatcatga
     1021 aaagcccccg gatgctcccc tcggacaaca aaaaagaagc tataacagcc ggagaaaatc
     1081 tcacagccat tatagcttct gttaacagat acctgcgtat ctgctgggtg tatggtaatt
     1141 agccttcgat ttcggaaacg acgccggcac cgacggtacg gccgccttca cgaatagcga
     1201 agcggagacc ggcttcgata gcaatcggag tgatcaattc gacgtccatt ttgacgttat
     1261 cgccaggcat gcacatttca gtgccttccg gaagctggat gacgcccgtt acgtccgttg
     1321 tacggaagta gaactgcgga cgatagccat tgaagaacgg cgtatgacgg ccgccttcgt
     1381 ctttcgtcaa gacatagacc tgtgctttga atttcgtatg cggatgaatc gtgcccggtt
     1441 ttgcgagaac ctggccgcgt tcgatttctt tacggtcaac gccacggagc agagcaccga
     1501 tgttgtcgcc ggcttctgcg c
//
LOCUS       DAWDXZ010000473         1518 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_13390_length_1518_cov_5.148325, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000473 DAWDXZ010000000
VERSION     DAWDXZ010000473.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13390_length_1518_cov_5.148325"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    899..908
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          372 a          347 c          387 g          402 t
ORIGIN      
        1 atccctacac actgtcccta aggggcaagg ccattgatac gtcttatatc gtcggcctgg
       61 ccacccatcc ggcagctcgg cgcggaggca ttggcggtaa actgttggcg gcggctctca
      121 cagagatgaa aagacgcggc cattacgtca atatcctcat gcccagtaag gccggtttct
      181 atcagcccta tggctatgaa ctctattgcc atcagtggaa agaaacgctg ccccttgaag
      241 ccctgcgccc gttgacggat cgtaccgtcc gctatgcctt cgtcaacagc agcgaccaat
      301 ggccatactt ggcagcagtg tatgacggct atacgaaggg gctttccggc tatgctctgc
      361 gtgatgaaaa aagctggcgc agccatatcg atgctcaatt gggagaaggc aatattgccg
      421 tctgtttcga tggcgatacg cctattggat atgcctttta tcaattggga caaccgacga
      481 ttgtcagtgg tgaatttgtt tacacctctc gtaaaggcaa ggccggcctt ctcggttata
      541 tgtataacca tcgttctcaa ggcgaagcct ttcagtggaa cgaaggcatt cacgaccagt
      601 cctatcgctt ctatcccgat ggaaaatcgg gccatgaaac gatgcccttc atgaccggcc
      661 gcattgtcga cgtcaaaggg gctttggaac agcagccgta tcgtgccgat gggaaagtta
      721 ccttccatac ggaagatccc ttggccgatt ggaactgcgg tacctttacc ttaaccgtaa
      781 ccgggggcag gggcattgtt acccgagaaa tgtcaaagga tgctgatgtt accatgccta
      841 tcggaactct ggccttattg gtctttggtg ccatggacgt aaacgacctg gtattcaann
      901 nnnnnnnntt cccgacagaa aaaaactgga tcaacgaatg gtattaaaaa cttttatgaa
      961 tgctgtctct ccgggggcag catttttgat atcggatacg gccttcgaca tcactctttg
     1021 gtaagatgaa aatgaaacgg gtttacatcg attgatatga cgataatagc ggtaactatg
     1081 ccgattttta ttttgaaatc gccatttgtt taatgaaata gtaaacttgt aaatgactca
     1141 tttcttagca ttttgcggtt tgatttttat gttttgttca aaatacagca gctatggagg
     1201 ttcgcgtggg taattgggtt cgtcgtcata agaaaaaatt tatacgaggc ttcgtggtct
     1261 tctttgtatt agctgcagcc ctttatggac tgtgctttta tatgagctac aaggctgccg
     1321 atatttttaa taagaccgtt gccgaacgcc aattgttccc ggggacggtc acggtggaac
     1381 gactgacagc gacaccgggt gggaccgttt cctttgaaaa cctgctctgg gtcgatgagg
     1441 acggaaagaa gttggtagag gtcccggacg gagagtttac cgtcaagata agcgatatcc
     1501 ttaccgggcg cattggga
//
LOCUS       DAWDXZ010000474         1513 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_13417_length_1513_cov_9.159122, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000474 DAWDXZ010000000
VERSION     DAWDXZ010000474.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13417_length_1513_cov_9.159122"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    421..457
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          365 a          376 c          375 g          360 t
ORIGIN      
        1 tccaactaca agacaggttt cctgaaaaag aacaagcgga acatccataa cgatgccgat
       61 tcattctaca tcatcaacag caatcgcctt cattcactgc tgctcaacgc cttccaaaat
      121 atgaaaaacc agccgtcgta ccgccagtac gacaaggacg cactcaataa gaaatacgaa
      181 acgtacaagc ccctcattga acggaccgta tccttatcca aataataaaa tatgcaggca
      241 acgaaaaaca ggctccatga ttcatggagc ctgttttata tcgcgattca gcttattttt
      301 tcaaatcttc tttcatttgt ttcaagccca tcggttcatc tctaaagagg aatgccggca
      361 tgattttgac atcgtcgata atcggagtga agcccatctg atcgaggacg tctttctgaa
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngct cgttccgtga tgtacaggac
      481 atgctgttta tgtttacggg catagttgcc gctgaaagta atatgaccga cttcgggaac
      541 aaatttcttg actttccctt cctgaaggat gtggagttca ccgtctttga tttcttcgcg
      601 gagtttcccc gtcgtaaagg taccgcagaa gacaacgctt ttcgtattct gggtgatatt
      661 gacgaagccg ccgcagccgg caatgcgcgt accgaattta gaaacgttga tattgccgtc
      721 tttatcgcat tcagcaaggc cgaggcaggt cagatccaag ccgccgccat catagaagtc
      781 gaactgtgta gcctggtcca tataggcttc ggcattaacc gaggcgccaa agcggacgcc
      841 gccgagagga atgccgccaa tagccccggc ttcgacggtc atggtcaggc tgtcgccgat
      901 gccttcttcg ttagcaacgg aagaaacgag ttccggaatg ccgatgccca agttgatgac
      961 cgcttcattg ctcatgttga ggagcaagag agccgcccgg cgggcaatga cttttttagc
     1021 attgagcgga gccggatcga gggctttgac gggaatcgtc gtttcgccgg tcagggacgg
     1081 atcgtattca caatcgaagg actgctggcg tttagcctca tcgtcaactt gaacgaggta
     1141 atcgacatag attcccggta cttttaccag tttcgggtcg agggtaccgc ctttgacgat
     1201 tttttcgacc tggacgacga cagtgccgcc gctgttatga acggcctgtg ctaaaggcgt
     1261 tgcgtcaaag ggagacactt ccttgctcat gacgacgttg ccccattcat cggcataggt
     1321 gccgcggata aaaccgacgt tcatatccat acgcggatag aagagttgtt catgaccgtc
     1381 gatttcgacg actttgacga agtcttcttt ggtaacgtca ttgaggcggc cgccgctgat
     1441 gcgtgggtcg gcaaaggtat cgaggccgac gtgcgtaatc gtgcccaggc gatgggccgc
     1501 cacgtcgcgg aaa
//
LOCUS       DAWDXZ010000475         1509 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_13451_length_1509_cov_6.497249, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000475 DAWDXZ010000000
VERSION     DAWDXZ010000475.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13451_length_1509_cov_6.497249"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    888..1014
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          311 a          386 c          357 g          328 t
ORIGIN      
        1 atgctcgacc aggtcctgga gttgttccgg attacgccgg attacgacct ggacatcatg
       61 gccaagggcc agaccttata cgatattacg acgaagtcgc tcctgggtct caaggatgtc
      121 ctggccaaag aacagccgga tctggtcctc gttcacggcg atacgacgac gacctttgcc
      181 ggggccctgg cgtcctatta tcagcagatc cccgtcggcc acgtcgaagc cggccttcgg
      241 acaggggata tatattcgcc gttcccggaa gaaatgaacc gcaagctcac cggggccatt
      301 gcggccattc attttgcccc gacctcgacg gcacgggcca atctcgtccg tgaaaatata
      361 gatccgtctc agatttacgt aacggggaat accgtcatcg acgccttgat gacgaccgtt
      421 gccggcgact atgacttcgg cgaccaattg aaatcggtgg acttcaagca ccaccgagtc
      481 atcctcttga cgacgcaccg tcgggaaaac ctcggcgagc ccatgcgcca tatcatcgaa
      541 gagattcccg atacggaaat cgtctttccc gtccaccgca acccgaaagt ccgcaaggtc
      601 gtcgaagatg aactgtccgg catcgaccgg atccatctca tcgaccccat ggaatatgaa
      661 ccctttgcca acctcatgag cctcagctat ctggtactga ccgattccgg cggcatccag
      721 gaagaagcgc cgtctcttgg caaaccggtt ctggtcctgc gcaatacgac ggaacggccg
      781 gaagccgtgg aagccgggac ggtccgcctc atcggcacgc ataaagacgt cgtctatgaa
      841 gaaacgaagc gcctcttgac cgacaaggcc gcttatgacg ccatgagnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttcctg
     1021 acgacttctg ccgttaaaat aagcttcagt tatattgaga atagttttta gctatatact
     1081 cgctttttct ttcattttgt gttgattttc ctgtttttcc gtgctaaaat aagcaataat
     1141 cgcggctaaa cgtaggtttt ctatgcgcgg cgcggttgtg gaaagactgc tatcgcctga
     1201 gcaggagtgt gtgtgctatg agaaaaaaga atgattctca cagtgacaaa aggtcttcta
     1261 tgaggctttt agacatcgtt gccattgccg catctctcgg cctgtcgctc tttgtctgca
     1321 tctgcgtcgg cgtcgccatc ggccgtttta tcgatgtcta tacgggcaca tcgccgtggg
     1381 gattgatcct cttttccctg atcggtgccg tttccgggtt ctggacgctt tacaaaaaag
     1441 ccttgtccta tgaacgggat gaatcccgcc ggaatggatc gtcatcgagc cgatgagaac
     1501 cgcggagga
//
LOCUS       DAWDXZ010000476         1507 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            NODE_13472_length_1507_cov_7.599862, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000476 DAWDXZ010000000
VERSION     DAWDXZ010000476.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13472_length_1507_cov_7.599862"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          421 a          276 c          368 g          442 t
ORIGIN      
        1 aagattcgat agttgagtca atgaaaaaat attcaaatta tttaggcggc atccattttg
       61 ctgatacaga acggcgtatt ccaggattgg ggacattaga tttttctgct attttagata
      121 cgcttcacag actccaattc catgggccgg catcgatgga agttaaacag attcccgatt
      181 cagaatcagc ggcctatttg agctaccgta caattcgcta cttagaagaa tggaatcggg
      241 cagtgtctgt ataaagtcta tgctatgatt tgacttcccg tcaagataga agttgcattt
      301 caatcctttg tggatgagaa tgcagggcgt gactcattgt aagtaaggct tttatttgtt
      361 attttacaac tcaatttatg aagcggatag ggttgaggaa tcttgtttct gggaaagtag
      421 tgtgattacg actgccaaca ggaatgagat tctttttttt tgctttgtaa attaccaatt
      481 gttttttata ctatccggtt tttgcatgga tgggaataga ggaatccaat tttacagaat
      541 ataaacttat ctcgtataat ttttacatag agaagcgatg tgaataagtc atctctacag
      601 ttcaatcaag taatgataga gcaataactg attaaaagat gagaaaggta gatgtttttc
      661 atggaagaaa aaatttttac acaagcacag ttaactgatt tggcaacacg acttttgaaa
      721 accggcgggc tgacggatga agcggcggcc ttgatttccg acgacttagt aagcgccgat
      781 atgcgtggat tgtatagtca tggtgtatcg cgtatcccta tgtatttgaa acgcattgac
      841 tgcaactgtg taaaaccggt tcctaatatt cagattgaaa aagtcggaca ggccgtactt
      901 aaagtaaacg gcgatgatgg catgggattc ttagttgccc ataaagcgat ggaagaaggg
      961 atggccttgg ctgaaaaaac gggtattgcc atggtcggat gcactcacag tacgcattat
     1021 ggtatggctg ccctttatgt aaaacaggcg gtagataatg gcttctgctg cattgtctgc
     1081 acgaattcca gcccggccct tcccgtattt ggcggacgta cggcgttctt aggggcagcc
     1141 ccctttgcag ccggcatgac tggcggatat aaaagtccgt cttatatatt ggatatggct
     1201 atgacgaaaa cggcacgcgg taaaattcgt gtagctatga tcaacaatga accgattgct
     1261 gaaggactgg cactcgatag tgaaggcaat ccgacgactg atgccaagaa agcttttgaa
     1321 ggcgtttgcc ttccttttgg cggagcgaaa ggtgccgggt tggctatgct gatggatatg
     1381 atggccggga tgtataccgg atcccactat gccggcgatg tcagcagcct ctattatcgt
     1441 tttgatgaac ctcaacattt aggtcatatc atgtttatca tgaaaccgga tatgtttatg
     1501 agtctcg
//
LOCUS       DAWDXZ010000477         1486 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_14451_length_1486_cov_11.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000477 DAWDXZ010000000
VERSION     DAWDXZ010000477.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_14451_length_1486_cov_11.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          367 a          359 c          422 g          338 t
ORIGIN      
        1 ctctcgactc ggggaaatgg agcgtcagtg acgtctatca cgatacgggt tccattcagg
       61 tagccgatcg gacgatgcat aactgggatg agaagggcct tggaaatgtc acccttcgag
      121 aaatcattat gtactctatt aataccggta tggtccacat tgctgtaaaa gcaggcggaa
      181 aattattgac cgaatatgct caccgtttcg gcttcggttc gattacgggc attgaacttc
      241 cgggcgaaca ggagggtatc ctattttcgc cgagtaccat gtccatcgtc gatacggcct
      301 ccatggggat tggccagggg atcgccgtca cgccgctgca aatggtccag gctttcagtg
      361 cgattgccaa tgatggccac atgatgaagc cttttatcgt gaaagaaatc gacaatcccg
      421 acggatcggt ttacgaaaag aaagaacccg ttgaagtcgg tcagcctatt aaggcggaaa
      481 cggcggaaac catcagtaaa tttatgggag aagaaatttc ttccggcggc ggccaaaatg
      541 caaaaataga aggatatacc tttgccggta agaccgggac agcccagaag aagacggctg
      601 acagcacggg ctatgccgaa gggcagtatg tcggttcctt catcggcttt ggtcctgtag
      661 aagatccgca gttcctggtt ttaatcgtcg tcgatgcacc gagcggcgtc tattacgggg
      721 ctcaggttgc ggccccggtc tttaaggatt tgatgacgga aatcgttcgc atgaaaggcc
      781 tgcggccgac ttccgataca ggacttaagc ccttggctcc gctggttacg gcgccgccga
      841 gagtcatccc tcctgtccat cagacagagg acggggtcct catcccgtcc tttatcggct
      901 ggagtacgcg ggaagtcaac gactggctta atgaagccgg cctgggcttt gttccaaaag
      961 ggatgggaag tgccgttcag caggatccga ggccgggaag ctatgcgcct tccggaagtg
     1021 acgtaacggt cacctttaaa cgctgaacgg atacagtatc atagttctaa ttagggggaa
     1081 tatcatgcaa ttactcaatg ggaaagatgt ggcggccttt catggcaagc gcattgccca
     1141 acagatacag gatgttcagg aaaaaaaggg gattgatcct gaacttgcca tcgtcgtcgt
     1201 cggtgacgat gcgccgtcgg ttatgtatgc ggcgtccatg aagcggactg ccgagtccgt
     1261 cggcctcaat gcccgtatct gtcaggagaa ggaaggggca ggtgaagacg agcttttgct
     1321 cctcatcgac aggctcaatg aggacccgtc ggtctttggc atcctgttga tgatgccctt
     1381 gccaaaatca attgacagtc agcgcatcat caatcgcatc gatccgtcaa aagacgtcga
     1441 cggactgact gatgctaata ttgccaatct gtatacggga agggac
//
LOCUS       DAWDXZ010000478         1486 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_24868_length_1486_cov_11.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000478 DAWDXZ010000000
VERSION     DAWDXZ010000478.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_24868_length_1486_cov_11.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          364 a          348 c          425 g          349 t
ORIGIN      
        1 aaaaaagtat ggaaagctaa cagattttaa tgtataatga gtttagtcga agatggcagt
       61 cgatgaactg ccggatttac tttttttatc cgttatttga acttgcgaat aacgatgcag
      121 ggaggcgttc atggaagggc aaaagcttca ctataaggtc atcatgtgtc tgttgggcga
      181 caagcgggct ttcggacccg gcgtggccga gctcttagac ggcgttcgcc gccgtggttc
      241 gctccaggga gctgctagag acatgaatat gtcttattca aaggcgtgga cgatcatgcg
      301 cgacgccgag gccatttggg ggtttcccct cattcgccgc tatgtcggcg gaaaagacgg
      361 gggacggtcg gtcctgaccg gacaggccga agaaatactc aagcgttacc ggcacatgaa
      421 cgaggccgtc cgagctgtag cggctcgaca gttcggcgag ttattcaatg atgaagaatt
      481 acaaaaatta agagaaatga gaggctagaa cgttatgaaa atgatgaaaa cggaagacgc
      541 tgtcggtcat attctctgcc acgatattac gcagattatc cgcggcgtta ccaaagcgcc
      601 ggtgtttcgt aaagggcaca tcatcaccaa ggaggacatc cccgttttgc tatcggtcgg
      661 aaaagaccat gtctatatct gggaaaatga tgacaccgtt taccatgaaa atgaaggggc
      721 ggccattctg cgggacatct gtatgggggc caacatgcat ggcagcgagc ctaaggaagg
      781 gaaaatcgaa ctcattgccg actgtgatgg tgtgctgacc attcaccgtc aaggtttgga
      841 agcggtcaat tccttaggcg agatgatgat tgctacccgt cacggcggct ttaccgttcg
      901 caagggcgat aaattggccg gaacccggat cattcccttg attataaaaa aagaaaaaat
      961 ggccaaggcc aaggccgtgg caggtccctt gccgctgctc gatgtccacc cttttcataa
     1021 gaaacgggcc ggcgtcgtca cgacgggcaa tgaagtcttt catggccgca ttgaagatac
     1081 gtttacgccg gtcattgaaa gtaagctggc tgaattcgat gcgaaaatgg ccatgcacaa
     1141 ggtcctggct gacgatccgg cagccattac ggcggctatc cgggagatgc ttgacgccgg
     1201 catggatatg gttttctgta cgggtggcat gagtgtcgat cccgatgatc agacgccgtt
     1261 ggccattaaa aacactgggg cccgcatcgt ttcctatggc gcaccggtat tgccgggagc
     1321 catgttttta ctggcctata cggacgacaa tcgacctatc gtcggccttc cgggctgcgt
     1381 catgtatgcc aaacggacga tttttgatct cgtcctgccg tcgctcatgg ccgatgttcc
     1441 tgtaaaggct tccgatttgt ctcgtctggg cgaaggcgga ctgtgt
//
LOCUS       DAWDXZ010000479         1470 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_22442_length_1470_cov_10.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000479 DAWDXZ010000000
VERSION     DAWDXZ010000479.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_22442_length_1470_cov_10.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          403 a          285 c          421 g          361 t
ORIGIN      
        1 ctccatgaag gatatcgacc accccggtga aaacgacatc cccttattgg aacgcagtaa
       61 atccgaaaac gatacagtgg tcgctcacgt tgaatggctg ccgacgacgg ccagccggac
      121 ggagttcgtc gtatttgacc agcatcagag aaaacagaac cggattaact ttgagtttgg
      181 cgatgaagcc ggtaaagaga tgagcagtaa cgatattcga atggatattg aacgtcatac
      241 ctatgccgac ttgtatggcg ttggcaaaaa ggataaagta gctaatatta ttaccggaga
      301 gttaacaagc aaagctagtg gatatgcgat tttcctggtt ccggctcgga gcggctacgg
      361 attctgggcc tatttaattg ataagcaaat aaaaaatcag gaggaaggca aggtaacctg
      421 gaaattctac ttgaagaaaa aacaatactg cagctttgaa aaagaggaac tggtaacgtt
      481 ctttgatggg aagcggtgag attgatgcta tttcacaaac agttggaatt tgcctccggg
      541 gctgtattga caaaagaaat gctggagctg ttggggagtt atccccggca gttcttgaaa
      601 ctgtacttca aggcctatag cgagggcatt ctctgcggct tggattatga gctgcgcggt
      661 gatgatcttt ggcttacttc tggggctttc tggtggcata atgagctatg gatgaccggg
      721 gaagatgtca atgtgagtgc ttttttgcaa aatcagtcac tgcaagaagg gcagtattta
      781 gttctgtgct ggcaggagga caaaaacgtt actgaaaaag gtgtcgacat ttgccctttg
      841 aaactcagta tagacactga ctctcgctat gagggtgtat tagaactcgg tcgcttcatt
      901 tggtgggatg acagaaaact gcaactgcca caaagttggg aggagctcat tgagtcgctg
      961 gaacgggggg attattttga tatttcgaat gccagtcagg ccgctctgag aggttctaat
     1021 gccagtcagg atgtcctggg aggcggaact tttatacgat tgattttccg atgcctgaaa
     1081 gaggctttgc ggaaaaagga aaggcgaaca tctttcgaag atgccatgtt ggtccaactg
     1141 caaagtcagg gatttttgtc catggacacc ttgtatacct acgtcggaac cgaggccggc
     1201 aggatgaatc ggagagagtt gttgaaggct gtagacgagg aacttcgccg tcaaccggcg
     1261 ccggtggtac aggccgcagc accgaaggag aatcctgatc atgaagatcg tccggatgaa
     1321 cctcgttggc cgatgggcgg catgctggat taacgatcga taggccgtat cctggaaggg
     1381 atgtgattct gtgaagaaag ataaggtttc gagggtatta gccctctatt cgcagttact
     1441 ggccggtcgg acggtacaaa aggcggtaac
//
LOCUS       DAWDXZ010000480         1436 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_14758_length_1436_cov_16.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000480 DAWDXZ010000000
VERSION     DAWDXZ010000480.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_14758_length_1436_cov_16.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          343 a          357 c          381 g          355 t
ORIGIN      
        1 catgtgactg cgaaaaaact gagcagttac attaaaataa ggacgtgtac ggcgaatgat
       61 tgacagaatt tttttatgga ggaaacccat atgtcaaaga aagaagtcag aacgatcgga
      121 gtattgacca gcggcgggga tgcgccggga atgaatgcgg ccatccgcgg ggtcacgcgg
      181 tatgctattc agcgcggcct gaccgtcgaa gggattattc gcggttatga aggtttactg
      241 cgtcatgaaa cgcgtccctt gggtcgacgc gatgtcggcg cgatcattca tcacgggggg
      301 actgtacttc ggacagcccg ctgcttggaa ttcaaagaaa aagaaacgca gcagaaagct
      361 gctgaatatt tacgggaaca ggaaatcgac gccttagtcg tcatcggcgg tgacggttcc
      421 atgcgcggtg ccgaagcctt ggccggcttt ggttttccga cggttaccat tcccgggacc
      481 atcgacaacg atatggtcgg gacccagtat accttaggtt tcgacacggc cgtcaatacg
      541 gtcctcgaat cggtcaataa gatccgtgat acggcctttt cccacgaccg cgtcgccgtc
      601 attgaagtca tgggccgcga agccggcttc atcgccctta acgccggcat ggcctccggt
      661 gccgaagccg tcttgattcc ggaacataaa gtcgacctgg cggcgctctg cgagcatttg
      721 gaagaatcgc accgcatgaa gaagctgagc agcatcgtca tcgtcgccga aggggctgct
      781 aaaggctctg acgtcgtcga ctatatcaag gaacatacgt atctgtcgcc gaacctgacc
      841 gtcctcggct acgtacagcg cggcggggct ccgtcggctt acgatgccat catggccggc
      901 cgctttgccc agaaagctgt cgacagcctc ttagacggca cttataacag tgtcatcggc
      961 ctcatcgaca acaaggtcgt tgccacgccc tatgaagaag ccgccaagct tcgctatccc
     1021 attgacgaaa acctctttga actggttcat ttcttaggcc gatagatatc ttctcacagc
     1081 ggctgcccgt cggcttttgc cgctgtgcct gcaaataggg aatacataag ggagccttcc
     1141 tttatgcatt cctttttttg tcgttttctg tcaaatacgg gcaaaaaagc ggcaggagat
     1201 gaagaaaatt tattaaatct gaattgacat cacataaata tgcaagtata ataagatagc
     1261 atgtgttttt gtatatttat ctgtatcatc aagaggagtg ttattcatgt catttgattt
     1321 tagcctgatt tggaattcat tgcctctgct gttggcaggc gccggtgtta ccattgaaat
     1381 cacggccatt gccgtcggct taggttttgt attcggcctg attaccagtg tgtgcc
//
LOCUS       DAWDXZ010000481         1420 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_50898_length_1420_cov_10.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000481 DAWDXZ010000000
VERSION     DAWDXZ010000481.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_50898_length_1420_cov_10.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          297 a          349 c          390 g          384 t
ORIGIN      
        1 acgagaacgg gggagtatca tggtacatca aaaggcaccg ggccagccga catttctgtc
       61 gattcgtcag ctgaccattg ccgctttttt atcggctatt acgatttttt tgggaattac
      121 cgggtacgga ttcattccgc tggtcatcat gaatgcgacg attcttcata ttcctacgat
      181 tatcggcagt ctgtcggcag gccgtaaggt cgggatgatc gtcggtttta tgtttgggat
      241 tttttccttt atccgttcct tgcaggcacc gagtgcgctg ctcttgtttg ccgtgcagta
      301 caacgtcatt gccgacgcct ttatttgtat tgtaccgcga attcttatcg gcctcattgc
      361 ttatgaattg taccgtcatt tgaagacgcg cgaagtggtg cgcgtagctg tgacggctgt
      421 cgtgacgacg gtgatccata cggttctctt cctgggttcc tttacggccc tcgtcggtgt
      481 tccttatgcc gaagcccaag ggattccctt catgaacgtc attaacatca tgctcggcat
      541 tacggctgtc aacggaattc ccgaagccgt cgtttccggc ctgattgtta cgcccattgt
      601 catggccctc cagcgtgccg ggttaaaggc gtagtaacag caaaatataa gggttatgaa
      661 tcaaacgggg ctgtccccca aagggcggtc ccgtttctct attctatgga aatttgctaa
      721 tgcgaaagtt tgcgctatgt ggtaaaataa gcaatgaatg gctgtttacg tatgacagca
      781 tatatgaaag gaatagaagg gattatttgt tatggcttcg attttttctc cattaactaa
      841 aaatataggc atcgatttag ggacgtctac ggtccaggtc tatgtggaag gccggggcgt
      901 cgtcttgtct gagccgtcgg tcatggcaac ggacgagcga aacagtaaga tgattgccgt
      961 cggaaggtcg gcagaggacc tcttgtcccg gtcaccggag acggttaagc tccatcgccc
     1021 gctcgtacac ggcgtcattg ccgattacag tgtaacgcgg acgatgctgg gctatatcct
     1081 cagcaagtgt gtccgcagcg ttcagcggct gcgcatcatg atcggcgtgc cgtcggctgc
     1141 gtcgaatgtc gaaaaaagag ccgtcatgga agcggtgtat caggccggtg ccaaagaagc
     1201 cttcgtcatt gaaacggcgg cagcggcggc gctcggtgtc aatttgccgg tctatgatcc
     1261 ggttggcaat atggtcatcg acatcggcgg cggtacgacc aatgtggcca tgctgtctct
     1321 cggcggcatc gtcgtctcca agtcacttca cttgggaagc cttgatttca atgcggctat
     1381 ccgcgactat ctgcgctacc agtacgacgt tgtcaccgac
//
LOCUS       DAWDXZ010000482         1415 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_46023_length_1415_cov_11.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000482 DAWDXZ010000000
VERSION     DAWDXZ010000482.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_46023_length_1415_cov_11.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          259 a          356 c          462 g          338 t
ORIGIN      
        1 gcgatgcatt cctttcctgt gtagcggtag aaataggacg ggttataggt cggttttccc
       61 gccaaatcga ggaagaagtc gcgcagggcc tgttcgtttt cccggatgtc atcgacgacg
      121 gcgtagactt cggagcggtt gcacgtcgac aggacaacgg cttcgcgaag gccgccgtag
      181 tcgtcgaggt gctgaaggct gctgccgatg gcgtccggtg tcatggcaaa ttgttcccga
      241 atggcgacgg ggaccgtttt atgattgagt ccgacgatga ctaaatccat ggctaaacct
      301 cgatgtcgcc gtttttcagc catcgggcga cgtcgatggc gtggtaggtg atgatgatgt
      361 ccgaaccggc tcgcttcatg ctgagcaggg attcggtgac gatgcgcttt tcgtcgatcc
      421 agcctttttg ggccgccgcc ttgaccatgg cgtactcgcc gctgacgttg tacgtggcaa
      481 tgggccgctg gcagcgttcc cgggcctggt ggacaatgtc gaggtaggcc agggccggtt
      541 tgaccatgat gatgtcggca ccttcgtcga ggtcgaggtc gatttctttc atggcttcgc
      601 ggctgttggc cgggtccatc tgatagcccc ggcggtcgcc gaagtgaggt gccgaatcgg
      661 ccgcatcgcg gaaggggccg taataggccg aggcgtattt agcggcgtag gacatgatgc
      721 tgatgtccgt gaaaccatga tcgtcgagag cctttcggat ggaagcgacg cggccgtcca
      781 tcatgtctga cggggcgatg atgtcggcac cggcttctgc ctggctgagg gcaactttat
      841 tgaggagttc caaggtggca tcgttatcga cttcgtggcc gcaaagtttg ccgcagtggc
      901 cgtggctcgt atactgacag aggcagacgt cgccgacgat gaccatgtcg ggcacggctt
      961 ttttgatggc cttcatggca cgctggaccg ggctttccgg atcccaggca ctggaaccga
     1021 tgtcgtcttt ataggccggc aggccgaaga tttcgatgcc tttgatgccg agttcccgga
     1081 tttcttcagc taaggcgacg gcattgtcga cggacaggtg gtaacagccg ggcatgctgg
     1141 gaatctcttc tttgaccttc gttccgggga cgacgaaaat ggggtagatg aaatcgttca
     1201 gggacaggtg attttcccgt atcatagccc gcatcggtgc cgaacggcgc agacggcggg
     1261 gacggagggt atttatggtc attgtttttc taatctccaa tcttttatac aatcagtcag
     1321 acctcttgtc gtgtaggtcg aggctgtcag gatatggttc aggccctgtt cgcggcacgt
     1381 ccgggccgta atgggaccga tgcaggcaaa ggtca
//
LOCUS       DAWDXZ010000483         1407 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_9643_length_1407_cov_14.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000483 DAWDXZ010000000
VERSION     DAWDXZ010000483.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_9643_length_1407_cov_14.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          336 a          392 c          356 g          323 t
ORIGIN      
        1 ctcaacgaga agaaaggaaa tgttatgctg catattggtt gtcacctgtc ggtgtcacaa
       61 ggatttttgc atatgggtga cgaagcttta tctataggag ccgatacgtt ccaatttttt
      121 tcgcgcaatc cgcggggaag caaggtgcga aagctcgata tcgatgatat ggaagccttg
      181 aatgaactca tgaagaaaca ccattttgcc ccgatcttag cccacgctcc ttatacctta
      241 aacggctgtt cccagaccga aaagacacgg gaatttgccc gtcaggccat ggccgatgac
      301 ctgcgccgcc tggaatacct cgataactgc cgttataatt tccatcccgg cagccacgtc
      361 ggccaagggc cggaaaaggg catcgacctc attgccgaca tgctcaatac ctgcatgttt
      421 gccgttcaga agacgacagt cctcttagaa accatggccg gcaaggggac ggaagtcggg
      481 cggacctttg aagaattggc ggccatcatc gataaggccg ttatgaatga taaaatcggc
      541 gtctgcctcg atacatgcca cgtctttgac ggcggctacg acatcgtcca tgagctcgac
      601 gacgtcctct tgaccttcga taaggtcatc ggtctcgacc ggctgaaggc cgtccacatc
      661 aacgacagca agaacgaact ggccagccat aaagaccgcc acgagaaaat cggtgagggc
      721 catatcggta ttgccgcctt tgatgccatc gtcaatcatc cggccctgcg ggccctgccg
      781 ttttatttag agacgcccaa tgaactcgac ggctatgcgc gggaaattgc actgcttcgc
      841 tccttacaac gatcggaata aacgtagata aaggggactt tttatgcgta tactacatac
      901 tgccgactgg catttaggaa aactatttta tggatcgtat ttgacggaag aacaggacta
      961 tgtattaaaa actcagctcc tgcctctcat taaagacgaa ggaatcgacg ccgtcgtcct
     1021 ttccggtgac gtctatgacc ggagccttcc gccgacggag gcggtcgccc tctttgatga
     1081 aatggcgacc aaaatcacgg ccgaactaaa ggtcccgttt ctcgtcatca gcggcaatca
     1141 tgacagcgcc gcacgcctgt cctttgccag ccgccttctg gcctcccagg gcctttatat
     1201 cgccggcgaa ttagaccgca tgacggatcc tgtggtgtta gacgataaat acggccccgt
     1261 cgccttttta ttggcccctt atgcagaacc ggccgtcgtc cgccatttct tgggcgatga
     1321 caaccttcgc agccatgagg cggccctttg cgcccttctt tcgtggcagc aggaaaagat
     1381 tccccgcggc atgcggaccg tttgcgc
//
LOCUS       DAWDXZ010000484         1394 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_34792_length_1394_cov_9.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000484 DAWDXZ010000000
VERSION     DAWDXZ010000484.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_34792_length_1394_cov_9.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          339 a          384 c          315 g          356 t
ORIGIN      
        1 acatgataaa tcatggatag tggcaggatg ctggttcctg tcgacattgg tattgtaccg
       61 aattcgtcca tttaacacaa ttcgaaatca aaaggcgttc cattagtttt acttatgtca
      121 aaactacggt ataatagcag taacgtttca aaagaaagga gtgagtccat gctgtttcgc
      181 caaatgaaat acttcatggc cgtcgtcgac gccaagagct ttaccgaagc ctcctatgcc
      241 ttggaaattt cccaatctgc cgtatcgcag cagatcaagg ccctcgaaga ggacttaggc
      301 gttcctttat tgattcgcca tcatcggcat ttttccctga cgccggccgg tgactatttc
      361 taccgccacg gaaaggaact gcttcaagaa atcgaagcgt tcaaggcgga aacccgcagg
      421 caaggcgaag ataaagagct gcacctatct atcggctatc tgaacaacta cgtcggcagc
      481 gagctctatg aaacgattgc cgcattttct gctgattacc cggaagtgac catccatatc
      541 ttcaatggaa ctcacgaatc cctgtactgg gaactgcgta cgcgaaaagc cgacttggtt
      601 ctcagcgacc agcgccgggc cttttccgac ttatacgaaa atgacattct cataaagggt
      661 acggtctatg ccgagatatc tcgtcagaat ccccggagtc aacaggaatt ccttaccttg
      721 gaagacgtga agcgcttccc ctgcatcctg gtcacggcgg aaaatcagga agcgtcagaa
      781 gctgcctatt ataccgagac cctgggcttt gccgataatt ttctctttgc ccgtaccatc
      841 gaagaagcac ggctcctcgt ggccggcaac cgcggctatc tgcctttgga aagtttttca
      901 ccgacgccgc cgtcgggcac ggcctcgcgc tgtctgcccc tctatcgagg cggcagtccc
      961 atgatgcgcc attactgtct cttttggcat aaggacaaca gtacctacta cgtcgaggaa
     1021 tttgcccgcc tgctccacca gcggattaag gagtaccaaa aagggtaacc ccgccaaaat
     1081 atacgtaacg ttgtcgttct atttatgaat tgtttaagct gtctctgtac cataaagtat
     1141 cattaccatt aggagggatc ttatgctttc tgccttacgg caccaccggc gcctggtatt
     1201 cgtttttttc ctgctggccg ccgcttttct ctacttcgcc cacatcgact cgtaccatct
     1261 catcgagctc gacgagggcc gctaccatcg tattcccatg gaaatggtcc tgtccggcga
     1321 ctatctgaca ccccatttca attacatgcc ctactttgaa aaaccgatct tccagtattg
     1381 ggtgacggcc ctgg
//
LOCUS       DAWDXZ010000485         1390 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_12435_length_1390_cov_11.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000485 DAWDXZ010000000
VERSION     DAWDXZ010000485.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_12435_length_1390_cov_11.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          351 a          326 c          395 g          318 t
ORIGIN      
        1 aatagagagt ataaaggagg gagcttatgg caacgcgatg gattatgcac gtcgatatgg
       61 atgcgtttta cgcgtctatt gaacaaagag acaatccggc cttgaaggga ctgcccgtcg
      121 ttgtcggcgg ccttatggac cgcggcgtcg tagcaacggc atcctatgaa gcgcggcgct
      181 atggcatcca ttcggccatg agcatgaagg cggcacggag acgctgcccg aacggcgtct
      241 ttttaccggt acggatggcg gcctatcgaa gggtgtcgca tcagatccgc ttgattttat
      301 ctcactattc tccctatatc gaacccttgg ctctggatga agcttttttg gacgtatccg
      361 gcatggaacg ccactatccc aatatcgtcg atatcggaaa agccgtcaaa gacgagattt
      421 ttcggacgac gggcctcgtc gcctcggcag ggattgcgcc caataaattt ttagcgaaac
      481 tggcttcgga tttaagaaaa ccggatggct tagtttggat tccctatggg acggaacaag
      541 cggtattggc gccgctgccg atcagtcgat tatggggcgt cggcaaggtg acggaagaag
      601 ctttaaagaa gggcggatac cataggatcg gcgatatttc ggcgtccagc ccggaggcct
      661 taaaacccct cttaggcaat caggccgagc gcatttaccg attatctctc ggtcaggata
      721 agcgaccttt ggaggtacgg cggcggcctc agtcggttgg aaatgagcat acgtacagcc
      781 gtgatttgac actgccttct gaagtcgatg aacagttccg cctcttagcc aatgaagtgg
      841 cctggcggct gcgtcagagc cggctcatgg gacggacgat tacgatcaag atccgctatg
      901 cttcctttaa gaccgttacc cggtcgctga cccttagcga tatggggacg gcgtcggaag
      961 aacaattata tttttcggca aaaagattgt ataataagga taaaggacat gagccaatcc
     1021 gactgctggg tctcaccgtc agccagcttc agccgtatca agtccagggc gatttatttt
     1081 ccgaagacga ggaaacgaag gagaaagtca tcgaggccat cgataaactg cagcagcgat
     1141 tcggtcgtac ggccattatg aaagggtttt tgtgggaaat gtcccatgag aaggatacgt
     1201 aaaaaaggag ttttagccat ggcaattttt aaagtcgtcg cccataccgg tgacaataac
     1261 aacgggtata tcgagtataa tacggaaacg aaggagctgg gcgttcatct gaatgatgaa
     1321 gctgtcaacg aacgcgcccg ggagtatttg acgaccgagc gggtcctgca tcagtatacg
     1381 gacctttcgc
//
LOCUS       DAWDXZ010000486         1387 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_40486_length_1387_cov_17.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000486 DAWDXZ010000000
VERSION     DAWDXZ010000486.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_40486_length_1387_cov_17.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          370 a          370 c          331 g          316 t
ORIGIN      
        1 gtccgccgca ttgaaaatcc tgatttcacc atcgtctatc tcggtgacgg cgttacggga
       61 tttcaactgg aattgacgtg gctcaaagac cgtaaagaac cgtacaacct cggcgacaac
      121 gaattccata cggcttttgc cgttaaggat atggatgctg ccttggccaa acataaggaa
      181 atgggctgcg tcgtctatca gaatccggct atgggtatct acttcatcgt cgatcccgac
      241 ggctactggc tggaaatcat tccggagcgt ggatgatgaa gaaagaatat acccagcgga
      301 cagcccgcct catcggtcag gataaagtcg atgagctcaa taccaagaaa gtcctcgtct
      361 tcggtgtcgg cggcgtcgga tcggctgtcg tcgaagcctt agggcgagcc ggcgtcggcc
      421 atctcatctt agtcgatgcc gacgtcttcg acccgtcgaa cctcaaccgc cagttaggcg
      481 ccaccgtcga cacgattggc cgatccaagg tagaggtcat gaaagaacgg ctgcttacca
      541 tcaatcccga tatcgacgtc gaaacccatg ccaccttcta cttgcccgaa agccatgaag
      601 gtttcatcgc caaatccggt gccgattacg tcgtcgatgc catcgatacg accagtgcca
      661 agatcgccat catcgacgaa gcctaccaca gcggaatccc cgtcgtctcc tccatggggg
      721 ccggcaacaa gctccatccg gagtacttca agctcgacta catcgaaaag acatccgtcg
      781 atcccttggc taaaatcata cgccatgaat tgaagaagcg ccgcatccgc cgcatcaaag
      841 tcgcctattc gacggaagta ccccgtacgc ccatctcgga cagcgaagaa cttcgtccca
      901 gcccgggcag catctccttc gtgccggcca cagccggtat gattttagcc ggtgccgtca
      961 tccgcgacct cctcgattta gattaaaata aaattttacc tatcttatac acaaaggctg
     1021 cgatctaaaa tcgcagtctt tttttacgat attttcacgg tatttcggcg attttgataa
     1081 gtatcggagc atacttgtcc acaacatgat agagtatttg cgcgttgaca cgacctacta
     1141 tatatagtat cataaatctt gtaaatatcg gccccgaaat cgtatatatt gtgggatacg
     1201 cgggacttgt ggacattttt atacaatata ctgtggaggt agacatatgc tggaagtaat
     1261 caagagaaac ggacaaaaag tgccttttga cagggacaaa attaccatcg ccattgaaaa
     1321 ggccatgaac agcagcagcg gcgtatacga agaaggccag gctgcgaaga ttgcaggtga
     1381 aatcgaa
//
LOCUS       DAWDXZ010000487         1382 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_32103_length_1382_cov_8.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000487 DAWDXZ010000000
VERSION     DAWDXZ010000487.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_32103_length_1382_cov_8.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          304 a          360 c          390 g          328 t
ORIGIN      
        1 ataaaaaaac agagtgtccc tacggggtct atttaggtcc cactttgtag gaccttataa
       61 aaaaaagaga cctgcccaag gcaaatctct tgtatcgtca gccgaaagct cccgaagggc
      121 cctcacagct tttttacgaa gcgcaggtcg tgccgcagtt tcccccttct tcagcagcgt
      181 cattggtcag tcccaggcgc ttgatcatgg ccatgtcttc gacgatggtc gagcccgacg
      241 tggtcagcat attgcccgta atcgacgccg aagcgccggc aaggagggcc gtagccccgt
      301 tttcaggtaa aagtttgcgg ccggcagcca ggcggatgtt ggcttcggga ttgatgaagc
      361 ggaagaaggc aatggtccgc aggatatcgt cgccggacag ttctttccta ccttcaaagg
      421 gcgtgccggg aatgggcatc aaggcgttga tggggatcga ttcgatgccg agttcggaga
      481 ggctaagggc catatccaag cggtcttccc aggtttcgcc catgccgatg atgccgccgg
      541 aacagacgca gaggccttcg gcctgggcat ctttaatcgt ccggatgcgg tcgtcatacg
      601 tatgggtcgt gcaaatctcc gggaagaatc gccgcgacgt ttcgatgttg tgatggtagc
      661 tggtaacgcc cgtttggcga agagcgtgga gctgcgctct cgaaagcaag ccgtgggagg
      721 cgcagagacc gatgtggagt tcccccttca tcgcccgata ggtgtcgagg gctttttcaa
      781 aatccttccc cgacaaggcc cggccggagg taacgatggc aaagcggttg gctccggcgt
      841 cctgattggc cttggcattg gccatgattt cttctttggg aaggaaatcg tattcgtcac
      901 agccagtatg atgacaggcc gattgcgcac agtatttaca gtcttcgctg cagcggccgc
      961 tgcggccgtt gatgatggtg cagaagtcga cgtgattgcc gcaaaaatgg cgctgcagta
     1021 cgccggcccc ttcttgcagt tcagccagcg gtgccgtcaa aaaaaggctc agatcgtcac
     1081 cccgtttgat gcgatagcct tgtaaaattc gttccgttaa acctgcgatt gtattcattg
     1141 ttaaccaatg cccctctttc tattagtgac tatgggatta ttatacgcgc tgaaaggcct
     1201 ctagtcaacc aattatttca cggtaatagg ttaacaataa actcgtactt ctccgtgaca
     1261 ggcgctgtca actcccctaa gggcacaaaa aaaggcccgt cgcagttgcg acagaccttt
     1321 tttcaagtgt tcttgccaat agcttatcgg cgttcgcgga tacgggcagc tttgccggta
     1381 ag
//
LOCUS       DAWDXZ010000488         1358 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_50143_length_1358_cov_15.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000488 DAWDXZ010000000
VERSION     DAWDXZ010000488.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_50143_length_1358_cov_15.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          365 a          328 c          371 g          294 t
ORIGIN      
        1 ggataaggcc ccatggcagg tctatgtcat tgccggcgac ggtgaaatcc aggaaggtca
       61 gatttgggaa gctgccatga gcgcagccca ttatcatctc gacaatgtca tgcttttcgt
      121 cgactgcaac ggacttcaga tcgatggtac caacgacgaa gtcatgaccg tcaatcctgt
      181 cagtgacaaa tttaaggcct ttggctggaa tgtcatcgaa attaacggtc atgattacga
      241 ggcaatccgc ggagctattg ctgccggcaa gagcgtcaag gataaaccga cggctgtcat
      301 cagccacagc gtcaagggca aaggcgtttc gttcatggaa aatcaagtgg gatggcacgg
      361 caaggctcca tcaccggaac agtgcgccca ggctctgaaa gaactgggag aaggaggcga
      421 agcgtaatgg ctatggcaac tcgggcagcc tatgggaagc tgctggaaac tgaattatat
      481 aaaaatcccc gcgtcgtcgt attggacgcg gacttaggga atgcgacgaa atcagcttcg
      541 tttaagaagg cagctccaga acgctatttc aatatgggca tttctgaagc ggatatgatg
      601 gggacggcag caggactagc gacagccggc aagattccca ttgccagcac ttttgctatc
      661 tttgcaacgg gccgtgcctt tgaacagatc cgcaattcta tttgctatcc taagttaaat
      721 gttaagattg ctgcaaccca tgccggcctt accgtcggtg aagacggtgg cagccatcag
      781 gcgattgaag acctttcact catgcgcagc ctgcccaata tgactgtcat caatccggcc
      841 gatgcgaaag aagcgcagga agccttgaaa gccgctgtcg actatgaagg ccctgtatac
      901 attcgtctcg gccgtgccga agtgcctgat atccacgatg attcctatca attccagtgg
      961 gggaaaggcg aaattttacg tgaaggcagt gacgtcggca tcatcgcgac gggcatcatg
     1021 gctgctaaag cgttggaagc tgcagatgcc ttggaagcca agggaatcca tgcccgggtc
     1081 atcaacatcc atacgctgaa acccttggat gaagaactcg tcatagaaac ggctaagatg
     1141 acaggccata tcgttacctg cgaagaacac agcgtcatcg gtggactggg atcggctgtc
     1201 agtgaagtat tagtacgcca ttgcccggtc aagcaggact ttatcggcgt tgacgattgc
     1261 tttggccagt ccggtaaacc ggcagaatta ttagaaaaat atggccttac agcagcggcc
     1321 atcgtaaaag cggctgaaac gaaataagtt catactac
//
LOCUS       DAWDXZ010000489         1350 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_28372_length_1350_cov_8.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000489 DAWDXZ010000000
VERSION     DAWDXZ010000489.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_28372_length_1350_cov_8.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          284 a          386 c          358 g          322 t
ORIGIN      
        1 cttcccacca gccggagccg ttcatgacga tggctgtctt gaactcttta aacaggctcc
       61 aagccccgac agccgtaaag ccgcccatgg aatgacccat gacagccaga ggcgtattgg
      121 gccaatgttc tgcaatatat tccagcatga ggggtacttc catcatgttt tggaagatcg
      181 tctgccaaaa atcagcatag acggccttgc tttcatagtc aagagtgccg cgatggccgt
      241 ggttgacggc atcgggaatg atgacatcgt atccgtaaga ggccaagata cggccgcgga
      301 cggcctgaaa ttccttctgc gacgaccagc cgtgataaaa caagacgacg ccttttgctt
      361 tttccccgga tgtaataacg agaccgggaa tccctttgag accaacttct ctttcttcaa
      421 tcatctatgg ttaaatcctc tctatttttt tataaatccc ttgcggtgcg ccaacggttc
      481 ctgccagatt tccgaccaga caaaaccggc atagagttct ctcgcttggg gcgtaagacc
      541 gctccaaggg gcaggacgga cgcgctgggg cacgacgacc ggcacagact tgggacgctc
      601 tgcagcgacc cgtggcggca ccatcttttt cggaagcgcc ggttccggtt catcggggaa
      661 attcatatta tcccaaccct catcccaatc gggaaaggac ggttccggca ctgtcggttc
      721 cggtgcttcc atctttgggg ccggggccgg cttacgccgg ggcggtacgg gaatgggtcc
      781 cttgctccgc gggtactttc gcttgcggcg caagaggtcc ggcaggaccc ccatcaagac
      841 gacgacgata atcatccata caagatccat ggtcatcacc tactgttccg aatggcttcc
      901 atcgtgatgg tcgtggtgat catcctttac ggtcgacgcc gactgggtct taggcacgcc
      961 ggcctgagcg acagccgtac gcatatccgt atcggccatg atattcttca tattgtaata
     1021 gtccatgacg cctaatttac cttcacgcaa agcctgggac aaggccaggg gaacttcggc
     1081 ctgggcttcg acgaccttgg cctgcatttc ctgggtataa gcctgcattt cctgttcctt
     1141 ggcaacagcc atggcacgcc gttcttcggc ctttgcctgg gcaatctgct tgtcggcttc
     1201 agcctggtcg gtctggaggc gggcaccgat attgcggcct acgtcgacgt cggcaatatc
     1261 gatggacaag atttcaaaag ccgtcccggc atcaaggccc ttattgagga cggtcttact
     1321 gattttgtcc gggttttcca acacttcaga
//
LOCUS       DAWDXZ010000490         1306 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_41502_length_1306_cov_12.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000490 DAWDXZ010000000
VERSION     DAWDXZ010000490.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_41502_length_1306_cov_12.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          316 a          354 c          339 g          297 t
ORIGIN      
        1 gtatcggcca tgatatcaac cttaccttcg aggaaatcgc gcatatgggt caaaatctgg
       61 ccgttgacgt cggtccctaa gtggcggata cgagccggaa tgaagctgag atcagctgca
      121 gcggcctttc gctttatctc gcgaatgcga tctttatttt catcgccgta caccttgcgg
      181 tcggcaccgc caaaacggca gtacacttcg tcgacataac ggattttatc cattaaatcg
      241 gcttttggca tatagtcatc gaggttgccg ccgtattcgg tagtcagtgt cagtttcccg
      301 tcgctgaagg caccggcacc gccccagccg cagacgacat tgcgcacccg atcctgcggt
      361 ttagcatcgc cattgagact ggtcgcaatc cgttcccgaa tatcctggcc tttttcgacc
      421 atgaggatat cgagtccctt atctgccagc tccaaggccg taaaaatccc ggccggtccg
      481 gcaccgataa tgatgacatc ataggttttt actgctttca tatacgccac ttccttataa
      541 aaacaccttt acatcgaaca aaaaaggcgt tgtctccttg ggacaacgcc ttttgcagcc
      601 acgcaagcag ctcctcattc agcatagcat ggtgcaccga caaagtcaac cggccgccga
      661 ttcgaggagg caagaaaaaa aagccgttcc cgaaggagcg gctttttcgt cacgcactaa
      721 gcttactgct gttcatgttc atgttcagct cgttcttttt tgtagaagtc agccgtaatc
      781 gcatcggagc agcatacagc gacgacgtca gctacgtctt cagacgagca gccacgggac
      841 aagtcaagga taggtttgtc gaggccctga accaaaggtc cgagagccgt tgcattggca
      901 aagcgctgaa ccatcttgta accgatatta gcagcatcga ggttcgggaa gatgaggacg
      961 ttggcatggc cggcaacggt cgagccggga gccttgtgtt cgccgacttc cggaacgagg
     1021 gcagcgtcag cctgcatttc gccgtcaaaa gcaaaatcga cgttgcgttc tttcaagatt
     1081 tcaacagctt tgcggacgtt ttcaacggat tcaccggcgc cggaacccat cgtggagtac
     1141 gaaaggaagg caaccttcgg attgaggatc tgaacggtac gacgagcacg ttcgacacaa
     1201 atgcaggcga tatcagccag ctgatctgcc gacggattgg ggataacgcc gcagtcaccg
     1261 aggacgagga taccattgtc gccgtactga ggagtgttcg tcaaga
//
LOCUS       DAWDXZ010000491         1301 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_15758_length_1301_cov_14.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000491 DAWDXZ010000000
VERSION     DAWDXZ010000491.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_15758_length_1301_cov_14.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          297 a          313 c          302 g          389 t
ORIGIN      
        1 ccctcaaaga gcgagccgac tatatcacag acgaccacga caagggcggc attttaaagg
       61 cctgtaaaaa attcggctgg ttataaggga tcgttccggc cggccccttt aaggagtgca
      121 tctttcagcc ggtcttcgat ctgaagcagt tcctgttcgg ctgcctgccg cttctgacgg
      181 ccttcacttt gaatgcgaag cgtatcttca atggtagcaa tcaaatcgtc attgacctgt
      241 tttaccgttt cgatatcgac gatggcccgt tcattttcaa cggccgtgtc atgagaattc
      301 tgcttgagca tggcggcatt gcgctttaag aggtcattcg tcgtattgga cacagcctgc
      361 tgcatctgga gggcttcctt ctgccgacta agaccgaggg ccaggatcat ctggttcttc
      421 cacaggggga tcgtattata aatagcactc tgaatccgat cgacgaggat tttgtcattg
      481 tcctggatga tccggatctg cggcgccgtc tgaatggcaa tggtcttact gattttcagg
      541 tcatgaacct tcttttcaaa gcggctgaca gaatttttaa aatcctggac aacctgtgtc
      601 gccatcgggt tgtctgaggc ctgagcctgg gcttccaatt gaggaatcgt cgtttcattc
      661 aattcctgaa ttttttcttc cccggcctgg atgtagagct ggagctgctt aaaatattcc
      721 aaattcttat cgtacagccc gtcgaacatg gctacgtcct tcatgaggat catcttctgc
      781 ttgtcgagac cggcctgaat attgtcgatc tgagccgaca aggtcaaata agactgctgc
      841 aatgccggca cctttttaaa taaggacccg acgacaggga tattcgccaa gaatccctta
      901 gggccatctt gttcttcttc aaaatcgtga actttcttta ccaggtcgtt gagaaggtca
      961 ccggctgccg tctgatcagt actctttacg gtcgataaga tgctgttgga gaaagacgcg
     1021 atattgtttt gggcagcaat gccgtattgg gcaatggcat tggccgttac caagtttatc
     1081 gactctttga tggtatcgac ttgtttccgt tcttcggctg taagcgtttc cacctgacga
     1141 ttaaaattga ccagttcctt cttttcatag cttttgggag tgccctcatc ggactgtttt
     1201 ttatccaaaa ggtcgttcaa attaatctcc gccataataa caccctactt tcgtttcgta
     1261 tttaaaaatt tatccgtcaa atcaatgatt gagttgacca a
//
LOCUS       DAWDXZ010000492         1290 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_44438_length_1290_cov_15.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000492 DAWDXZ010000000
VERSION     DAWDXZ010000492.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_44438_length_1290_cov_15.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          339 a          287 c          284 g          380 t
ORIGIN      
        1 gcgggtgttt tgcgaatagt aacggtcttt atgatactgt ttggtatgag tggacgatgc
       61 tggcgaagtt tcacccagcc gacttttggc agtttgatct tgccatcttc gatacgtacg
      121 gaccccttct ggttattcgt agaatatgcg aagcagccgc tctttcggga cttgaaacgc
      181 ggcttgccaa aatgcttcgg attgttccag aaattattgt aggcccgatt gaggttcaac
      241 tgagcattgg ctaatgccag actgtccact tccttgagcc agggatattc tgccttatac
      301 tgtgccggcg tattgttcag ctttttgccg gtcttctggt aacagtctag gcagtcatcg
      361 agcatcttat tgtagatgaa ccggacacag ccgaacgtct tggcaaacag gcttttttgt
      421 tcgattgtcg gatacagacg atagcggtag gccagattca tacgattcac cgtccttgat
      481 gttcgatgta ctttcggatg atttctatgg gagcaccgcc agggggcaaa aggaaaaaac
      541 tacgcgacca gaacatctct ttccagagct tttgacggac ctccgaaaaa tcctttttga
      601 tgagtctgga gctggcacgc ttataggcgt tgatgaactt tgacagttcc gtattcggat
      661 ggccacgaaa taggatatgt acatgatctt ggtcatgatt ccattcttcc aaggtgatat
      721 gataagaagc accgattctc acaaaaatat cctttgaaaa ctgactcagg gattcggaaa
      781 acaccttgcg tcgatatttc acgaataaga ccaaatggta atgaagcaag aatactgaat
      841 gattattatt gtctaattcc atgatatatc accaccttat gccttatacg actgatgaca
      901 tcatagaatg acactgcagc caatctgtct ggctccatta cttgcgtaaa ttcgccagac
      961 ccgcgttaat cccctacctt aagaggtagg ggattaacgc ggagagatta aatgcgaatt
     1021 atcgtgaacg aaagcatccc attccttgat atcagactct gaatatcctt gaatatcctg
     1081 ccatttatag ttgggcagga tgcaagttgc atgattaaaa cctccattac acggtgtttc
     1141 aatatacact tttacggttc cgtctccctg gattgacgaa tgcgtaatct ccgtatcgtc
     1201 cgccaaagtc atgtaagggt acatcataag cgttcctccc tgatattaat gtttattttt
     1261 attatatcac acccggcctc attagaatca
//
LOCUS       DAWDXZ010000493         1288 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_8689_length_1288_cov_10.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000493 DAWDXZ010000000
VERSION     DAWDXZ010000493.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_8689_length_1288_cov_10.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          347 a          287 c          358 g          296 t
ORIGIN      
        1 gagagtatca tgtctttttg aaaaaactat tgacattatg acattattag cgtactataa
       61 acgtatgaaa aagacaattt agcatatacg gtttaatgaa ccggcaggag gtaaagaaga
      121 tggcaatgga tagagaaaag gcgtatgtat tatggtttga tgaattgcgt agagctgacg
      181 tagctctcgt aggcggcaaa tcgtcgtctc tcggtgaatt gacgtcccag acggacgttc
      241 ccgttcctta tggttttgca acgacggctg ccggataccg tcattttatg catgaaacgg
      301 gcatggatgt gaagattccg gaattattaa aagggttgac cgacgtggaa gatgccgttc
      361 agcttcgcga tgtgacggca aaaatccgtc aggctatctg tgatgctcag atgccggccg
      421 acttagcgga agaaattcgc aagagctatg aagaattagg taagaaagtc ggcgaagcgc
      481 agccttttgt tgccgtccgc tccagtgcta cggccgaaga cttgcccgat gccagctttg
      541 ccggtcagca ggacacgtac ctcaatgtcc agggggccga tatggtcatc cagaaagtaa
      601 aggaatgcta tgcttcgacc tttaccgacc gagccactta ttaccgcacg aaacagggtt
      661 ttgaccatct gtccgttgct ttgagtgcgg ctgtccagat gatggtgttc tccaaatcgg
      721 ccggtgtcat gttcaccgta gatttggtta ccggtgacga tacgacgatt accattgaag
      781 gggcttgggg ccttggcgaa tacgtcgttc aagggacggt tacgccggat aacttcaaga
      841 ttcgcaaaga cgacttgtcc atcgtatcgc ggatggtcaa tacgaaaccg gtcgaattag
      901 tccgtaagcc cggcggcgac tgtgaagaac gcctcgttcc gccggaactg cagaaacagc
      961 aggtcattac ggatgaacag attcgagaac tggctcaata tgctaagaag attgaaaaac
     1021 actacggctg ctacatggat atggaatggg gcgtcgatga acggaccaat aaggtatggc
     1081 tccttcaggc tcgtccggaa acggtttggt cgaagaagaa taaagaaaag gccgaaacgg
     1141 ctgcggcacc tgctgcagtg acgacagacc gcaaggttat cgtaaaaggc cttccggcaa
     1201 gtccgggcag tgctgccggc aaagctcacg tcatcctcga tccgtcgaag attgcggaat
     1261 tcaaagacgg cgaaatcctg gttacgac
//
LOCUS       DAWDXZ010000494         1271 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_19439_length_1271_cov_11.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000494 DAWDXZ010000000
VERSION     DAWDXZ010000494.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_19439_length_1271_cov_11.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          323 a          294 c          342 g          312 t
ORIGIN      
        1 atccatgcta ccggaacggg acatgtcatt tccgtagagg ctgcagaaga tattcagtta
       61 aatagagaag atacgattga agacaaatat cctatcattt attggggcgg tgagaatgac
      121 ctttccttat catcggggaa caatattgtc atgaacaata aggacgacgt cgttatggtt
      181 tatgttgagc agcaggggag agtgcatctc gttgcggaca atgacattat ttttaatagc
      241 gatggaaggc ccaatgaagt aaatggccat caagccgtga tacatatcga agccggtcat
      301 gacattatcc attatcagaa ccatgaaaac agcaaccccg tttattattt aggggatgag
      361 acggaggctc acgtcaaggc cggccacgac ctgattatag acagcctcta ctcgaccaat
      421 ctgtttaacg tcaaggacgg ctcttcggca gacgtcgtag caggcaatga tattgtcttt
      481 aaggtgaaga ccttggcttt gactcccagt gcttacagca tgtataattc aaacctgtct
      541 ctccaaagca aaaacttttt cagtaatgcc cagcgaactt tgcgaacccg cgacgattcg
      601 caattgacgg tccgggccga tcatgatctc gtctttgata cgccgggggc tattgatgag
      661 ctcagtatga ttgccgtgaa tgccaggagt acggctgacg tctcggccgg tcatgatatg
      721 gtgctaaagg ttggcgtcgc agcccctaac gtccatgtcg atacgaaggg gacggctaat
      781 ttgacggccg gtcacgatat gatcctttcg acggccggta aggatggcga aaatccgctc
      841 gaatccaatc tctatgccga taatggcacg attacggcaa aggtcatgga cgggacgttg
      901 tccatctcaa ataatggtgc cgtcggagct tatgctaata acggcggcag cattaccttc
      961 gaaggccctg tcgtcatgaa aaccggaagc ggtgccgttg ccgccaatgg agggaagatt
     1021 catttcaacg gtcccttggt gattgacagc accgatacgg ctttgcgggc ggtagatggc
     1081 ggctccattg actttaaggg gagcgatacg gatcaataca ttgaaggcga tgtcgttgct
     1141 gctgacggcg gtatcgtttc catcgatttg gagaaggccg gctcctattt gaccggggca
     1201 acctcggtat cggatgatcc gactgcttct gtcgactcgg attcatccga tcagtatcag
     1261 gaccgggctg c
//
LOCUS       DAWDXZ010000495         1265 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_41396_length_1265_cov_10.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000495 DAWDXZ010000000
VERSION     DAWDXZ010000495.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_41396_length_1265_cov_10.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          296 a          323 c          338 g          308 t
ORIGIN      
        1 gagcgaaggg cgatgatgct cttgccgccc ggcgaacgat aagcgccgtc cgtaaagtca
       61 agctgtccgc cgctgccgct gatctgctgc gttcccgacg attcggaaga aacctggccg
      121 aagaggtcga cttcgataca gttattgata gagatgaagt tatccatctg ggcgataacg
      181 aaaggatcgt tgatgacgtc taccgggtaa gctgccaagt acgggttatc atccatccag
      241 tcatagaggt cctgcgtgcc gagggcaaag gaccagctta ccttattggg gcaaaaagcc
      301 ttgcggcggt tagtcagctg accgttctta tacatcagat agaaggcgtc gacaagcatt
      361 tccgtgtgaa cacccaaatc cttcaagtcc gattcagaca agagggcgcc gatcgtattc
      421 ggcaggctgc cgatgcccag ctgcagggtc gcaccgttag gaacctgttc aacgatcatt
      481 ttggcaatgg tagcatcgat atcgtccccc gtggcaaagg gaatcgttgc caacggatga
      541 ttgccgtatt cgacgatgcc gtcgatgtcg ctgatgtgga tgcattcacc gcggccgccg
      601 aggacgcgag gcatagcttc attgacttcg acgatgactt tcttagcctt ctgggtaatc
      661 gcttccgttg ccgatacagc caggccgaag ttgaagaacc cgtgtttatc catagggccg
      721 accgtgatga gggccacgtc gacgtcaagg aggtcacggt acatggacgg tttattgcga
      781 tagcacatgg ggatgaagtt catctggccg agtgcgcatt ttttccggtc atagccgccc
      841 atatgccagt tcatggccgt aaaggctttg cggtccggat cctgttcaat gatttgacgg
      901 ggagcgacgg aaagacacat gcggactttt acgtcctgca gttcgtcccg ccgggctgcc
      961 aaggcctggt cgaggagaac cggctgggtc gtacccatac cgtaatctac ccagtcgccc
     1021 gattttacga ctttaaccgc ctcttcaggg gtcatcaatt tttgtttgta catgtctgtc
     1081 cattgagaca tagaaatcac cctttccata gtatctatac gaattgcata gtacgacgtt
     1141 ttacgtttac ttatagtcat attatacgcc gcacagccag tcgttacaag cgttacagta
     1201 aagtatcaca tgctaattac gcatagttgt cccggatact gccatggaga cagaatttgt
     1261 acaca
//
LOCUS       DAWDXZ010000496         1247 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_26722_length_1247_cov_11.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000496 DAWDXZ010000000
VERSION     DAWDXZ010000496.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_26722_length_1247_cov_11.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          308 a          310 c          346 g          283 t
ORIGIN      
        1 ccagattcgg gacatttatt cccagttata agtgaatttg tactttttgt attagagaat
       61 tgaatggtat agagaattaa atcgagaagg agtgatgtag atgcgtattg tagatttact
      121 caagaagcag agcattgacc ttaatgccgc tgttgccgat aagacagcgg ccattggcca
      181 cctcgtcgac ctcatggatg ccggcggtaa tttgaatgac aaagagctgt acaaagaacg
      241 tgttctggct cgtgaagccg aaggcagcac gggtatcggc gaaggcattg ccattcctca
      301 tgccaagacg gaagccgtaa acgaaccggg gctggcctcg atgatcgttc gtgacggcgt
      361 cgattacgaa agcctcgacg acgaaccggc tcatctgttt ttcatgattg cagccccggc
      421 cggcggtgca gacgtccact tggaagtatt gagccgtttg agccgtatgc tgatggatga
      481 tgattttcgc aatgccttga tgcaggctaa gacgccggaa gaataccttg cgacgatcga
      541 taaggccgaa gccgaacaga ttgaagccga agccgcagaa gcggccgctg aagccgctgc
      601 ggaagaagca ccggccgtag aagcggctcc atccgaacga cccttcatcg tcggcgttac
      661 ggcttgcccg acgggcattg cccatactta tatggctgcc gaagcgttgg aaaataaggc
      721 cgctgccatg ggcgtcgaca tcaaagtcga aaccaatggt tccggcgggg ttaaaaatca
      781 cctgaccgaa gccgatatca agcgggctgt cggcgtcatc gtcgccgtcg ataaagacgt
      841 accggtccag cgctttgccg gcaaaaaggt catcttcacc aaagttgcca acggcattaa
      901 gatcccggaa gaattgattc agacagtcct cgacggcaaa gctcccgttt atcagggatc
      961 ggaatcgtct tctgaatcgg tagctcttga aagtgccgaa aaggaaagcg tcggccgcca
     1021 gatttataag catttgatga atggcgtatc ccatatgctg cccttcgtta tcggcggcgg
     1081 tattctcatc gccttggcct tcctctttga tatggacaat gccggtgcgg caaacttcgg
     1141 tagtgggacg cctgtcgccg ccttattgaa aaatatcggc ggtctgtcct tcagcatgat
     1201 gatgcctatc ctggccggat acattgccat gagtatcggc gaacgac
//
LOCUS       DAWDXZ010000497         1245 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_37081_length_1245_cov_10.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000497 DAWDXZ010000000
VERSION     DAWDXZ010000497.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_37081_length_1245_cov_10.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          298 a          302 c          347 g          298 t
ORIGIN      
        1 cctgacggtt tggctgagtg cggacggtac gatgtcatca aagtgaatgt acaagaattt
       61 ttgagcgccg gcctgtccat atcggccatg ttggagcggc tgcagcttcg cgttatccgg
      121 gaaatctgcc gggcctaccc cgatgtcaat tattttgacg atacccattt ggccgatacc
      181 ttaggcgatg tctttgccgg tacgagacgg cagtttgtca tcatcttgga cgaatgggac
      241 tgcatctttc gggaatatcc ccacgatgaa agggcgcagc ggctgtatct cgattttttg
      301 cgcaacctgt taaaggataa ggagtacgtg gccttggcct atatgacggg gattttgccg
      361 attaagaagt acgggacgca ttcggctctc aacatgttta cggaattttc catggagcat
      421 cccgatcaga tggcacagtt cgtcggcttt acggaagacg aaacgaagaa tctctgcctg
      481 cgttacggta tggattttga agaatgccgg gcttggtacg acggctattc cttcccgaat
      541 tgtccccatg tgtacagtcc gaaaagcgtc gtcgatgcca tggtcaaggg ccgtttcggc
      601 agctattgga acaagaccga gacctatgaa gcactgcagg tctatatcga tttgaatgaa
      661 gacggcctgc gcgatgctgt cgtcaagatg atgggcggcg gctgtcaaaa aattaatacc
      721 gggaccttcc agaatgacat gacgaccttt acttctgccg atgacgtcat gaccctcttg
      781 gtccacctcg gctacctcgg gtatgatagc gagggggaag aagtctttat tcctaataac
      841 gaagttttac aggaatacgt caatgccacc aagcaaggcg acgcctggca gcccgtcagt
      901 caggccgtaa gagcgtcaga tgagctgctc caagccgtct ttgacggcag ggcggatgac
      961 gtggctttcg gcgtggcggc agcccatgtg gaaacgagtc agattcagta caacgatgaa
     1021 aatgccctca gttacacact gtccctggct ttttacagtg ccaggaggta ttacaatatc
     1081 gttcgtgaaa tgcctgccgg aaaaggcttt gccgacctgg ttttcgtgcc gcggccgcag
     1141 catggggata aaccggcctt agtcgttgaa ctgaaatgga atcatgatgc cgagacggct
     1201 atcagtcaaa taaaggaaaa aggctacgcc gatgccctgc agcac
//
LOCUS       DAWDXZ010000498         1232 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_31400_length_1232_cov_10.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000498 DAWDXZ010000000
VERSION     DAWDXZ010000498.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_31400_length_1232_cov_10.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          315 a          343 c          309 g          265 t
ORIGIN      
        1 atacccttct cgaacggtcg gactacatta cgatgcacac gccgctgacc aaggaaacga
       61 caggccttat cggtaaggat gaaattgcca agatgaaaga tggcgtgcgg gtcatcaacg
      121 tctcgcgcgg tgccgtcctc gacatcaatg ccctggccga tgccttgaag agcggccacg
      181 tagccggcgc tgccatcgac gtatttccga cggaaccgct tacgacggat atcaatccct
      241 tcgttggcat ggataatgtc gtcatcacgc cgcatctcgg cgcttcgacc gtagaagcac
      301 agatcggtgt ttccgttgac gttgccgaag gcgtcatgga agccttgaag ggcgaacccg
      361 ttgctacggc tgtcaatatg gctcccgttc cgcctcatgt ctacaacgtt atccatcctt
      421 atttcgacct catggaacgg atggggaatt taggcgtata tttggccgat gcacccatga
      481 aggaaatcga agtcgaatat acgggcgaac tggccgatac ggaaacgcgc ctcttgacga
      541 cggccgttct taaaggggct ttaaatccca tcctgcagga atcggtcaat tacgtcaacg
      601 cttctgaagt ggctaagacg cgccacattg ccgttaagga aatcaacagt cagtccaaca
      661 actactttgt aaactcagtt actgtccgcg ttcggaccaa taaaggcgaa cacgccattg
      721 tcggcgtcct gttcaatggg acggaagcga aaatcgttca gatcgatgaa taccgcgtcg
      781 actttaaacc ggaaggctac ttgctcctcg taccccacgt cgaccagccg aatatgatcg
      841 gtcagatttc gaccatcctc ggccaggctc atatcaacat catgggcatg caggtcggcc
      901 gcatgacgac gacgggcaac aacatcatgg ccattgccgt ccaggatgat attcctaacg
      961 acatcatggt caagctccgc tcgatcaacg ggattctcga cattaaactc attcactgtg
     1021 acacacacta aaaggggaag attatggtac gcatcatact tattcgccac ggcgaaacac
     1081 aatggaacat tgaaggccgc taccagggac aggaagatac tcacctcagc gaacggggcc
     1141 agctccaagg ccgcatggtt gctgacggct taaagaatac gcccattgac gtggctattt
     1201 caagtccgct cgaacggtcc tacatgacct gc
//
LOCUS       DAWDXZ010000499         1229 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_738_length_1229_cov_11.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000499 DAWDXZ010000000
VERSION     DAWDXZ010000499.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_738_length_1229_cov_11.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          266 a          332 c          326 g          305 t
ORIGIN      
        1 gaccgattat atctcgtcct ttggcagcgt agccgtcttt atcatgatcc tgctcatcgt
       61 gctgaccaat atgaccggtt tgccgtcggt ccagttcgtc accgtcaacg gcatcctctt
      121 cggcctcgtg ccggggattc tcatctcctg ggccgggcag gtcatcggca acgtattggc
      181 tttcatcatc atgcgctacg tctttcggca taaggcccag gccttagtcg ataagagccg
      241 atggttgtcg cagctcaacg ggaaaatcga ctgtaagctg gtcttcttct ttcgggccat
      301 tccctattct cccaactttg tcgtcacggc ctttgcagcc ctgagccgca tcggttttgc
      361 cgaacacggt gcagcgacca tggccgggaa aatcctcgcc gtctgtatgg aagtctggct
      421 gggctacgac ctcatccatt ttaaccccca cagcctgata gccgtctgtc tgatggtctg
      481 tcttttggcg gtcctgctct tcggcagccg ccgctggctg aagcataaaa gtagtttaaa
      541 ataatatcac atatatgtag gaggtatttc ttatgggctt aattaaggca gctatcggcg
      601 ccgcaggcgg tgtcttgggc gaccagtgga aagagttctt ttactgtgac agcctgaacg
      661 aaacgatttt gatggctaag ggccaaaagc gaatcagccg gttcggccgc agcagcaata
      721 cgtcaggcga agacaacatc atttccaacg gctcgcgcat tgccgtcaat gacggccagt
      781 gcatgatgat cgtcgaatcg gggaaggtcg tcgaagtctg tgccgaatca ggggaatata
      841 cctacgatac gtcgacggaa ccgtctatct tttccgggga ctacgccggg gatttaggcg
      901 aaatgctgca tcagctcctg tcgcagatga aggaacgctt tacctttggc ggcgacgccg
      961 gaaaggacca acgggtatac tatttcaact taaaggaaat tatcggcaat aagtacggta
     1021 cgcccagtga agtgcctttt aagacctatg acgatacgac gggccttgcg ctcctcgtgc
     1081 gcctgcgctg ttttggcgag tacagctacc gcatggtgaa ccccatcttg ttctatacga
     1141 atgttgccgg caatgaagcg gatttttatg accgcagccg tctcgacagc cagctgaaat
     1201 cggaactgct tacggcactc caaccggcc
//
LOCUS       DAWDXZ010000500         1217 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_46436_length_1217_cov_9.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000500 DAWDXZ010000000
VERSION     DAWDXZ010000500.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_46436_length_1217_cov_9.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          294 a          304 c          343 g          276 t
ORIGIN      
        1 atgtaggccg tcggttcgac gtccttggca ctcagggcca ggcttcccat ggcggtaagg
       61 gtcagcccta aagcgagcca gcgtttccat tgttttttca tagtgatcat gcacctcctg
      121 catatttcaa cccattgtat caagacgtca tcaatgtttt gtgatgaacg ggtaagggct
      181 gtgtaaaaac gaggatggtg tataatggta gcaaaggcat tgaaaaggag acagggacat
      241 gacggaacaa gaatgggaac aacttttaca aatcaagacg gccggccggg acgacagtca
      301 cgctgacggc gagcatcatc cctatgagcc gacggactac ggtgtccttg aaaaattggc
      361 caacagcggc tctatccgca agaaggatac ccttatcgac tatggcagcg gcaaaggaag
      421 ggtcagtatc ttcatgacct atcagaccgg atgccgctct atcggcatcg aatacgacga
      481 acggttgtgg cagaaagccc tggtcaacgc caagagtccg gcagctcgaa ataaggtgtc
      541 ctttatcctg gccgatgcag cggtgtatga cctgccggat gaagccgact gctgtttctt
      601 cttcaacccc tttgccctgc acaccctgaa aagggtcctg gggaagatct tcgattcttt
      661 gcagcgtcgg ccgcggcggc tgcgaatctt cttttactat ccttatgaag acgtggaaga
      721 ttttctcagc caccacctgc gcctcgtggc agaagacccg atcgactgca gcgacctctt
      781 tggcggcgat gcgaaagaaa aaatcctcgt atatcgcgtg cgagaggagc tgtaagacct
      841 tagcgatttt gctgattccg gagataacgg gccgcacttt accgcagggt atcgagttca
      901 tccgattgga tatggtcgtc gcatagcggc aggatctgat cttgcaagac ggaatccggc
      961 gccgttttca gcctttccgg gtccagccag ggaaaggcga gatttacttt ttccggagtc
     1021 tccttttccg gtttggcagc gggaatgcgg ttgtaagggc aggcattttg acaggcatca
     1081 cagccgacga cccaagaccc catggctttt tcaggcagcg caggcggcag ttggcttttg
     1141 ccgaaggtat tccaaaaaga gacgcacttc aaaggattca tggtaaaggg ggctgaaaga
     1201 gcccgagtcg ggcaatg
//
LOCUS       DAWDXZ010000501         1212 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_3104_length_1212_cov_10.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000501 DAWDXZ010000000
VERSION     DAWDXZ010000501.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_3104_length_1212_cov_10.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          270 a          321 c          342 g          279 t
ORIGIN      
        1 atcgacgccg ccccactttt gcaggagcga gaaaataggc agctgcggcc acgacgtgac
       61 gtcgatatcg acgccgacgc cttttggcag gacgcgggga atattttcat agaagccgcc
      121 gccggtgatg tggaccatgc ctttgacggt aaagtttttg atgatcggca ggcacggacg
      181 cggatagagg cgggtcggcg tgagcaggca ttcaccgatg gtcatgccca attcatcgac
      241 gtactggtcg agtttcatct gctggcgttc aaagacgatc ttacggacca aggagaaacc
      301 gttagaatga acccccgtcg acggcaagcc gatgaggacg tcgccggcct tgatcgtttc
      361 gccggtaatg actttgtcgc ggtcgacgat gccgacgcca aagccggcca aatcgtagtc
      421 gtctttagca tagaagccgg ccatttctgc cgtttcaccg ccgaggagag cgcagcctga
      481 ttctttgcag gcattggcta ccccttttac aatggaggca acctgaaccg gatcgagctt
      541 gccgacggcg atgtagtcga ggaagaacaa gggttccgca ccttgaacga ggacgtcatt
      601 gatggacatg gccacgcagt cctgaccgac cgtgtcgtgc ttgtccatca ggatggccag
      661 gcgcagtttc gtaccgacac catcggtacc gctgaccagg attggctttt taatatcgct
      721 gtccatcagc gaaaagaggc cgccaaaacc gccgaggtcc ccaaggactt cgggacggta
      781 cgtagctttt acagaatctt tcatcaattc gacggcttta ttgcctgcgt cgatatcgac
      841 accggcatcg gcataggtca aacctttatt tgtgttcgag agcatatttt tctccttcct
      901 cgttcgtctg cggcgtatcc gtaggataat catcattgaa gcaagctaag cacatatctt
      961 cctgaggaat gtgttcaatc gcccggttca ggccgtcgat ggaaatgaag tgcagcttgt
     1021 cgacgccgat gtatttttga atctgttcct gactgaaggt cgacgcaatg agttccttgc
     1081 ggaccgacgt atcgatgccg tagaagcagg gatatttgat aggcggtgcc gaaacgcaca
     1141 tatagacttc cttggcaccg gcttcccgca ggagcttaca gatgatgccg ctcgtcgtgc
     1201 cgcggacgat gg
//
LOCUS       DAWDXZ010000502         1209 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_21618_length_1209_cov_10.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000502 DAWDXZ010000000
VERSION     DAWDXZ010000502.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_21618_length_1209_cov_10.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          281 a          326 c          319 g          283 t
ORIGIN      
        1 gcgcaagaga gccttgatct tagctactaa aattttcggg ctgaaaggct tggtaatgta
       61 ttcgtcagct ccgagatcgt aggcaaagag ttggtcaatt tcttcacctt ttgccgtcag
      121 catcatgatg ggaaccgtcg attcgcggcg gatttcacgg catacggtcc agccgtcgta
      181 accgggcatc atgacgtcga gaaggaccaa atccggatgt tccttgtcgt agatatccag
      241 ggcttctttg ccgtcaacag cttctaaaat ttcgtagcct tcaggcttta aaaaatcaat
      301 gaccaattgg cgcataagtt gttcgtcatc tgcaataagt atcttttcca tagttaatcc
      361 ttccttctga tagcgtctgt atctggcagc tgagcgagcc agtcatcaat cggccggcca
      421 aagaggatga ggtccggcac cagatcccgc aaggggacca atacgaaagc ccgttgtgtt
      481 agataaggat ggggcaaggt cagctccggc gtccggcaga cctcgtccgg gctgtaaacg
      541 aggtcgatat cgatggttcg ggcaccccat ttttcatggc gcacccgccc cagctgccgt
      601 tcaatggcaa gacagcgttc gaggagcttc tggcccgtaa gactcgtctc cacggaaacg
      661 gcaccgttga taaagggcgg ctgatcttcc ttcccccacg gcggcgtttc ataaaaagga
      721 gacaccgtct taacggttac ttcaggcgtg gcatcgagga gggccacagc ccgtttcagc
      781 gtctcttccc gattacccag attggcgccg atgctaaggt aaaatttagc catgtcgtcg
      841 cctttcaagg actacggccg catcgtcaaa gagaccgcca atcggagcct ggggcttatg
      901 gacggttacc gtcacgacct cgacaggagg aaaggcgccg agaatctctt cggcaatgac
      961 cgtacccagc cgctcgataa gctgatagcg gcggttctcg acgatgtcct taacccgagc
     1021 atagacgaca ctgtagtcga cggtctggtg aaggtcatca gaggcgccgg cctgtgacag
     1081 atccgcttca aggcatatat cgatatgaaa cggctgacca agctccgctt ccgatgctaa
     1141 gcagccgtgg tgcccccaaa agtgaagtcc tttgagcatg attttatcca tcttgttttt
     1201 cctccatga
//
LOCUS       DAWDXZ010000503         1189 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_47561_length_1189_cov_12.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000503 DAWDXZ010000000
VERSION     DAWDXZ010000503.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_47561_length_1189_cov_12.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          237 a          303 c          382 g          267 t
ORIGIN      
        1 cccgaaccgt aaggtcggga cggtaggcga gaagctgggc ccttcggcgc aggctcgacg
       61 tgccgacgca ggcctcctgc ggcagggcgt cgagggatgt atagcgattc gataccaaga
      121 cgtcgtggac atgagacctg gccgtgacgg cggctaagca gagaccgtcc gggaggtcgg
      181 caggcaaatc tttcaggctg tggacggcta aatcgatggt accggtgagg agccgcgatt
      241 cgagttctgt cgtaaagagt cctttgccac cgatgcgggc caaggggacg tcgaggatct
      301 tgtccccttt cgtaacgata tgctcgaggg ttacggtcag ttccgaatcg tgaccttcga
      361 gggctttttt gatgtattcg gcctgccata aggccagggc gcttttgcgg gtgccgatgg
      421 tcaggttacg tttcatggcg gctcctttca aattcgataa gcttttggaa taggcggcgg
      481 ccggcttcgt cgatgtcatt ggcttcgacg aggtccatga tgtcgtccga aagggccgtg
      541 cgccaaaaag catgacggtc tttcgaggac ggcagggcct ctttggcagc acttctgacg
      601 cgggccactt cgtcgagcca gcgactgtag cggtcatcaa agcgtttttc taatcgttgc
      661 cgcagccagc gcgagaaggc cggccccttg ccgttcgttg aaatggcaag ctgcaagtcc
      721 ccttggcgga cgacggccgg cagggtgaaa ctgcatagtt cggggacatc gcagacgttg
      781 atcggtttac ctgcttcttc agcggcctgc gaggctatac ggcttacggc ttcatcatcg
      841 gtggcgcaga tgatgaggaa gaagggcttt tcgtcgccca cctcataggg acgggccttc
      901 cacgtgagcc ggtgggaagc ggcccagtct tttaccgtgt tcgttgccgt cggagcgatg
      961 atggtgacgg cggcgccttc gtcgaggagg cgggccgctt ttcgttcggc aacggtaccg
     1021 ccgccgatga ccagacaggg gcggtttcgg agatctacat ttataggata catagggaat
     1081 cctcgctttt ttataatata ttccttatta tacacaattc tccggaaaaa agcgtggcaa
     1141 cgacaacact tttggaaaga gggatatgag aaaatccgta cagtgcggg
//
LOCUS       DAWDXZ010000504         1176 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_20037_length_1176_cov_10.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000504 DAWDXZ010000000
VERSION     DAWDXZ010000504.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_20037_length_1176_cov_10.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          327 a          249 c          290 g          310 t
ORIGIN      
        1 acggataacc gcaaccgcgc cgctgccgac gttcgccacg aattttctaa atccggcggc
       61 aacctcggtg ctacaggctg cgtcagctgg atgttccaca acacgggtct cttcgtcgtc
      121 gcccgcgata aggctgacga agatcagatc atggaagtcg ctatcgaagc cggtgccgat
      181 gacgtaaaag tctccgacga agactacgaa atcatcacta cgccggaagc cttcgatgcc
      241 gttgaaaaag ccctggctga caacaacatc gaaacggacc atgcgtcgat caccatgatt
      301 ccggaaaaca cgatcaaact gaccggtgaa gatgccgaaa aaatggaacg cttcatcgac
      361 gccctcgacg aaatcgacga cgtccaggac gtgtaccata acgcagaact tccggaagac
      421 gaagaataaa acaagtaaaa cagcaaacag cgcctgcctc atttctataa atgggacaag
      481 cgctgttttt gcgtgtggga tttttgtaat ggccgatacc attaagacca tcgcctatgg
      541 gggctacatt tgtgatttgg ccaggtgcag tagaaagcga tacggaccat tttcgcggca
      601 tcacgaaaat ggtccgtgtt gatgatttct ttattattcc tctgtaactc ctactttccc
      661 tatgaatcgt ctaaatagta aaaactgcgt tttttataat ttcaattttg cgagattata
      721 aaaatggcca tttttactat ttcatttttc tgagattaca aaaaatgtac aatgtacaat
      781 ttggctagaa tacaaaaaag gagtcttgtg aaccgatggt cgcaagacta tttttttata
      841 aagtccgaaa agggttgagt agcctgcatc gattcataaa tatcaattta ccttgggcag
      901 gattttacga ctggctgtcg aaattgtgag gtatatggaa tgagggagac cggcttattt
      961 tttttacgaa agaggtggat gatggtgttt cggaaaattg tcttgctgtt ggtgatgttg
     1021 accttctatg ggatgagtgt tttcgccggg aatatggttg aggtggatcg tgctgtgctg
     1081 aagattccca agacggcgaa tctatcgaca cacgttgatt tttatgaagg gaaacagagg
     1141 gtgcgctttg ccggcggccg cgtctatggc gataag
//
LOCUS       DAWDXZ010000505         1170 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_33933_length_1170_cov_12.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000505 DAWDXZ010000000
VERSION     DAWDXZ010000505.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_33933_length_1170_cov_12.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          253 a          309 c          319 g          289 t
ORIGIN      
        1 ccgattgcat cgacagccgt gatgacactg gctgcgtcgg ctccgacgac gaggttgcgc
       61 atcagtaccg atttgctccg cgtttcgccg tattcgtccg gataggacaa gaccagcttc
      121 aatttgctgg atactgcact gcgttctgcc atgattcatg cctcctttct ttaaaaatta
      181 tgtgtgaatc gggtatacac ccgttacgtc aaacaaggtt atgcggcgtc cccccagagg
      241 gcggcgcctt tcatctggac ggggtactgc atgatgcctg tggcaatggc cctggccgcg
      301 gcatctttat gcgcctcgta gcggcgccag tcgcagtcgt tgtcaaggaa acagacttcg
      361 acgaggacgg ccggcatcat ggtgctgcgc aggacgaaga gccccggccg ttctttgagg
      421 ccccggttcg ggaggttcaa gacggctttc aaattggact ggatacagtg ccccagcatc
      481 aaacttcccg tagagcccga gatcagcgtc tccgtcccgc tggcacggcc gttgaaagcg
      541 ttaaaatgaa gggaaataaa gaggtcgcag tcttcactat tggcctcgtc acagacggca
      601 tagagattgt cgttctgtcc gaagcgtacg gcaataccgt tatcttcgag gtaggacatg
      661 acgaggcagg ccaattcata ggctgcgtcg cattccctga ggtcaaggcg cggattgacg
      721 gcgcccgaat caagctgccg gtcgtggccg gcgttcacat agactttcat aatgctcctt
      781 tcttaaaact cacttcttgt tccgatttct acactggctg tttggccgcc gatggcgatg
      841 ccctgaatgg ctttcttttt ataaggacaa ggacaggtat cggtccgccc gcattcggca
      901 taaggcctga tgggaagcaa gagagcgctg gccctttgca ggatgaaaat gagtcgcctc
      961 ttgtcgaggt ttcgcaagaa acgaaagagt tggttcataa tatctccttt ctttaggcaa
     1021 ttttacggat gactaaggta atcatgccgt tatcattcaa ttccatatag gtccggtaag
     1081 gacttccggc agcgatttca gactcccggg tactcatgca gttcatgata tcgagacagt
     1141 tgcgcctagc cgtcgcttcg ctgatatccg
//
LOCUS       DAWDXZ010000506         1165 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_16956_length_1165_cov_8.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000506 DAWDXZ010000000
VERSION     DAWDXZ010000506.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_16956_length_1165_cov_8.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          282 a          324 c          309 g          250 t
ORIGIN      
        1 atatcgtatc gggcgactac tcgcagattg agctgcgcct tttggctcat ctctctcagg
       61 acccgaccat gatcgacggc ttccagaaag ggcaggatat ccaccgccgg acggcgtcgg
      121 aagtcttcgg cattccctgg gatgaagtta cgccggaaga acggtctcac gccaaggccg
      181 tcaacttcgg catcatttac ggcatcagtg acttcggcct ggctcgtaac atttccattt
      241 cccgtcagaa agctaaggat tatatcgatt cctactttgc ccgctacagt acgattcacg
      301 attatatgaa cggcctcatc gaatcggcga aacagagcgg tatggccgtc accatgttcg
      361 gacgccgccg ggccctgccg gacatcaaca gcaagaattt cacgcgccgt tcctttgccg
      421 aacggacggc catgaacacg cccatccaag gcagtgccgc cgacatcatc aagttggcta
      481 tgaacgccgt ccaagatgag ctggaaaagc ggaagctgaa aagcaagctc ttggtccaag
      541 tccatgacga actggtctta gaagtgccgg ctggcgaaaa agaggaaatt gaaaaacttt
      601 taaaagatac gatggaacac atcgtcgacc tttccgttcc cctcgtcgta gacattcaca
      661 gcggcgctaa ctgggaagaa gcgaagtagg tgagtttatg ccggaattgc cggaagtaga
      721 aacgatacgc tccttcatcg aaaagaaggt acagcacaag gtcatcacgg ccgtcgacgt
      781 atccctgccg cggctcatcc gcaatacgac ggcagaaaat ttcgtttcgt ccctgaccgg
      841 aacggaaatc gaaggcgtca gccgccgtgg aaaatacctt tttctccgct gcagcggccc
      901 ttggtcggtc ttggtgcacc ttcggatgac gggcagcctc ctctatgaag ctatgcctgg
      961 agagtatgcc ggacgggccg ttcacgtcgt cttttctctg acggaaggcc gactcctgta
     1021 tcgcgatatc cgaaccttgg gctgtttatg gctcgtaccg acagaaggac cgacggggat
     1081 caaaggctat gacaacctcg gtcccgatgc catcagtccg gcttttacag cagagggctt
     1141 gtatcgtttc ttaaaggaaa cgcgc
//
LOCUS       DAWDXZ010000507         1155 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_5858_length_1155_cov_10.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000507 DAWDXZ010000000
VERSION     DAWDXZ010000507.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_5858_length_1155_cov_10.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          327 a          259 c          329 g          240 t
ORIGIN      
        1 atgctatatt ctaatccgtt ttcgaaaaaa atcaagaggt ttttggttga aagttataat
       61 ctatgtaaag aatctgcata gttgtcataa aatttcgcca aatgtcaaag actttttggc
      121 aaaaataaaa ctgcaaaaag acgggctgct atctgctgta gaacccgctt ttgcagaatg
      181 tcgcccggca gttgacctct tacccggcag agtgtggtat gctgtgggta cagagcaaag
      241 cctgcaccgc cgggcgggga acacaaagta gaaaaaactt gaaatgatct gccgaaaatc
      301 ctttttattg gactgcaaat gtggataata gaagcataac gagggacgga catcccccgg
      361 aaggcttgaa cgacaagaac atttgtggag gataatacga tgaaagattt tgattttggc
      421 tacttcggca ccggcgatga gggctatgcc cagtacatga ccgcattcga gcgcaatttc
      481 agcacggaca gcaccgacag ctctgcgtcc gatgacacag actgcgatgc agacagctgc
      541 gacagcagcg aagaggattt ctaaggaggt aaaaccatga agtattatgt aacactgacc
      601 ggcctgaact accgctacgg cacgatgccc tttgcagtag ggcagaaggt gcgtctggtc
      661 aaggagccgg agaatcaggc cgaccgcgaa gccatccggg cagagctgcc ggggctgggc
      721 aaggtgggct atgtggccaa cagcacccat accgtaaagg gggactgcta cagtgcaggc
      781 cgtctgtacg ataagatcgg caacaccgct gtggcaaagg tgaagtacat cctgtgcgat
      841 gccgtgatct gcaaggtaaa ggcagacacc gcagcggccg tgccgcccat gaaccccgac
      901 accggcctgc cctatggcag cgaagaggac gctatcgcgt tttaaggaga atgtctttat
      961 gggtaaattg acgatacgag gcgaggctgc aaaagaatat gcctacgacc tgatggaaat
     1021 ttcggtgcag ttctggggaa cagagggctc tgcggctgct gctataaaga aagtgacgca
     1081 tcagtcggaa gaatttctcg aagagttgga acgcgctggc gttaagccgg aggatattca
     1141 tatcggagag gatgc
//
LOCUS       DAWDXZ010000508         1142 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_21736_length_1142_cov_7.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000508 DAWDXZ010000000
VERSION     DAWDXZ010000508.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_21736_length_1142_cov_7.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          306 a          255 c          284 g          297 t
ORIGIN      
        1 ataaaacacc gacggtcgtc atttcccgcg gctaatgcaa aaggctgtga agagagggtg
       61 aatcctttct tcacagcctt ttgtttttgt tgacaaatac tgacatatgt catatactta
      121 ggaaaaagat tgatgacata tgttgaaaat agaaatataa aaaattcgtc aagttatggc
      181 gtatttaagg agaatctgat atgatgaaga tagctgttac ctatgaacat ggtgatgtat
      241 accagcattt cggccattcc tcacagttta aactgtatga tgttgaagat gggcgcctgg
      301 tcgaagctcg catcgtcgat acggaaggtc aaggccacgg tgcactggta tcctttttaa
      361 cgtctctggg cgtcacgacg gtcattgccg gaggaatcgg tgacggtgcc agaaaggccc
      421 tggccgatgc ttccatccaa ttgtatggcg gcgtctcagg cagtgctgat gaagccgtca
      481 aagccttgtt ggccggaacc ttggagttta accccaaccc gtcttgcggc catcatggcc
      541 acgaaggcgg tcaccatggt catcatgaag gccaccatca ttctcatgac ctttgattgt
      601 ttccctgaaa ggagcggatt atcttgaaaa atataactgc tttttactta catagctgcc
      661 cgtactgtaa aggtgctaaa gaagcccttg ctgaattgat tgaagaaaat ccggaatacc
      721 gcaaggttac cgttgactgg tatgaagaaa cggaaaatcc cgaagcggta aaaggccatt
      781 cctactatta cgtaccgtcc atgttcatcg gtaccgaaaa actgtatgaa gcgcagccgg
      841 gccagtctta cgatgaaatt aaagctcacg tcaaagcggc cttagatgcg gccctgacgg
      901 cttaatcgtc acccaatacg gcgtgaacgg cttgtgcaca gcgacggacg gcttcgtcca
      961 tggtttcttt ttcgatggtc aagggcggat taaagtaaag gatgtcgcca atagggcgca
     1021 ggactaatcc caaatccatg gctttgcgga aaatgtgcca gcctaagcgt ttttccggat
     1081 tgaacgggcg tttacttgct ctgtcttcta ctaattcaat agcgttgatc aggccaatgt
     1141 gg
//
LOCUS       DAWDXZ010000509         1129 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_28818_length_1129_cov_9.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000509 DAWDXZ010000000
VERSION     DAWDXZ010000509.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_28818_length_1129_cov_9.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          318 a          270 c          297 g          244 t
ORIGIN      
        1 accgaagtct ttcgcaatct gggacagtcg ctgatggaaa ttctctatac gccgcggttg
       61 aatcccgaca atattcgcca atacgtcacg ctcgaccatc cggaacgact cgatgaagcg
      121 atgaaagaag ataaaggggt catcgttctt accgcgcata tggggaactg ggaatggctc
      181 ggtgccttag ccatgtatgg ctatccggca tcgacgattg tcaaaaacca ggctaatagt
      241 gccgttaccc gactgctcaa tgaaaatcgg gaaggcatgg gacttgaagt ctttgcccga
      301 ggcggtaatg aaatgatcat cgccgcccgt gccctgaaac gaaagaaact cctcggtttt
      361 ttggccgacc aggacggcgg cttttacggc gtgccgcaac ccttcttggg gaagatgagc
      421 tcgacgccta agggaccggc catgtttgcc caaaaatttc actcgcccat cattccggtc
      481 tttaccatcc atgatgaaaa ccaccgtacg cacctggcta tcggagaaat catgcattat
      541 gaagacacgg gggataagga agccgatatt gcccgcatga cccgtaagat ggcggttatt
      601 acggaaaatt tcatcaaaga acatccgacc gaatggctgt ggttccagca tcgctggagt
      661 acggagccgg aagagatcat tgcattacaa cagaaaccgg aggcgcatat cgatgatagc
      721 cgaaaagctg aaaaagaata agatttccct gattgccgtc atcgcagtca tcggctttgc
      781 tgtcatgctc tacgtcttca tgaatcccaa cacgtcggag gatacgatgg taagtcctga
      841 taagctcgtc gagttcgatg ggtcggaact cgaagaaaaa caggatggca aactggtctg
      901 gagactgacg gcggataaga ttcaaatcga tccggatacg cagatcatgt attttacgaa
      961 tccgaaagca ctggtcgtcg ccgaagacgg aacgcagatg accattacct ctgataaagg
     1021 cattgtcgac cgggaaaagc gtaccattga aatcaagcag ccggtcaagg ctgaaaccga
     1081 taagggcgat acgttgcaga ctgaaggcag tgtgtattat aatatggat
//
LOCUS       DAWDXZ010000510         1126 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_29838_length_1126_cov_8.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000510 DAWDXZ010000000
VERSION     DAWDXZ010000510.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_29838_length_1126_cov_8.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          302 a          236 c          318 g          270 t
ORIGIN      
        1 cgacggattt atctttggct ctcctgtcta ttatgcccat ccgagtggtc agatcttgtc
       61 tttcttgaat cgtgtatttt actcgtcggc tgttggggaa ctctatccgg tctttgccgg
      121 caaaccggga gctgccatcg tttcggcccg ccgcggcggt acgacagcgg ctgtcgatgt
      181 cttgaacaaa tactttggca ttgccagcat gcctgtcgtt ccgtctacgt attggaacat
      241 ggttcacggc atgacgcccg atgaagttcg caatgacttg gaaggtcttc agaccatgcg
      301 caatatcggc cgcaatatgg cttggatgtt gaagggcttt gccaaacaag gggcagaaag
      361 cacttttgcc gaccatatcg aaacagacca tcggaccgat tttaatcatt aaggctgacc
      421 ttaaggtgtg atatgactcg ataagggaac cgcgttaaat ctttgcataa caaaacggtt
      481 gctatcctaa gggacgaact cttggacagc aaccgttttg tcataatact gatttaaatt
      541 agactatttt tgcactgctt tactagatta aagaaataag gtaaagctat cgtacaaaag
      601 tctgacttac caggggggga gataagctaa actttattta ttaagaagac tgtggctgcg
      661 gctgtagccg tagtaaatga tcaagccgag aacgagccag ccgatgaaga gcatatgggt
      721 cagcggcgac agttcataaa tcaggtagaa gcagacgata acgccgatgg gagcaatgat
      781 cggaaggatc gggcagcgga atttccgcgg gatttccgga tggttgcggc gcatagcgac
      841 gataccgata aagttgatca agaatactgc cagcgtaccg gtgttggata attcgacgat
      901 gtgcatcaag gggacgaagc cggagatgat ggcgatgacg atcgaaccaa cgatggtaat
      961 caggtacggt gtgtggtacg tatcatgaat cttgcagaga tgctgcggca ggaggccgtc
     1021 gcgggagact gcaaagaaga tacgggactg gccgaagagg taaacgataa gggtcgtaat
     1081 gataccggct aagacgccga ttgcgatgag gttggcaaga tcaggc
//
LOCUS       DAWDXZ010000511         1109 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_30240_length_1109_cov_10.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000511 DAWDXZ010000000
VERSION     DAWDXZ010000511.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_30240_length_1109_cov_10.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          296 a          287 c          280 g          246 t
ORIGIN      
        1 caacttcggt tctgtcgacg gtgactcggc ggcagccatg cgttatactg aagtccgtat
       61 ggcaaagatt acgggagaaa tgctggaaga tatcgacaag gaaacggtcg atttcatgcc
      121 caactacgac ggctctatgc aggaaccgac ggtcttgccg tcaaaaatcc cgaacctctt
      181 agtaaacggc tcggctggga ttgccgtcgg catggcgacc aacattccgc cccataattt
      241 aagtgaagtc gtcgatggtt gtgttcagct tatcgacaac ccggattcca gtctggaaga
      301 aatcatgacc aaggtcaaag gccctgattt tccgaccggg gctaaaatca tgggtcgtga
      361 cggcatcgtc aaagcttatt cgacgggccg cggcagtatc aagatgcgtt cctgcgcttc
      421 catcgaaccg atgaataagg ggaagaatcg tatcgtcgtt acagaaattc cgtaccaggt
      481 caataaggcc cgggtcattg aatccattgc cgacttgtcc cgcaataagg aaatcgacgg
      541 aattacggct cttcgcgatg aatcggaccg caaagggatg cgcatcgtca tcgagctccg
      601 cgccgatgtt aacccggata ttgtcctgaa caagctgtat aaacacactc aactccagga
      661 aacgttcggc gtcatcatgc tggctctcgt cgacggtcat ccgcgggtct tgacgctgaa
      721 acagatcttg acctactatt tggatcatca gaagaatgtc atcacgcgcc gctgccggtt
      781 tgaactcgat aaggctttgg cccgcgctca catcttagaa ggtctgctca tcgccctcga
      841 ccatatcgat gaagtcatta agaccattcg tgaatcggca acggatgaag tggccaagac
      901 gtccctcatg tccaagttcg gccttagtga aaaacaggca gttgctatcc tcgatatgcg
      961 tctgcgccgc ctgaccggtc tcgaacgcga aaaaatcgaa gaagaatata aggaactgga
     1021 agaacgcatt gcttacctcc gcagcgtttt agccgatgaa cataaagtca tggaaatcgt
     1081 aaaaacggaa ctcctcgatg ccaagaaga
//
LOCUS       DAWDXZ010000512         1108 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_13902_length_1108_cov_21.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000512 DAWDXZ010000000
VERSION     DAWDXZ010000512.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_13902_length_1108_cov_21.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          217 a          310 c          324 g          257 t
ORIGIN      
        1 gtcccttcgc gaagacgtgc tgcgccgcga ttttaccgtc aacgccttgg ccatggacag
       61 aagcggtgag atttacgact atgtcggcgg ccgaaaggat ttgaagaaga aacggctggt
      121 taccgtcggc gatcccgtgc gtcgttttca ggaagatgcc ctgcgcctct ttcgcctgtg
      181 ccgttttgcc ggtcagctcg attttatggc ctccaaggaa accgtagctg ccatgccgtc
      241 ggcctttgac cgcgtttccg gcttgtccct tgagcgagtc gtcagtgaac ttgaccgact
      301 gatggtgaca ccggcttctt ttcgcggctt ggatattctt gtccgcagcg gccttgcagc
      361 ctgttcgtgc cgtcaaaaag aaaatggggt ttatacggcg attcccatcc tgccggaatt
      421 aggtcacttg cccgatacgc cgcagtcgaa gcccttccat ctttttgatg cctgggtgca
      481 taccttggcg acagtggctc atacgccacc tgatctgact gttcgctatg ccgccttatt
      541 tcatgacatc gccaagggcc tgcccggcat ccgcggtgaa aagaaaggac gttataccga
      601 ttactgccac gattcgctgg gggccgatat ggccaaagag attttgctgc gctggcgtaa
      661 gaaaccggcc tttgccgatc gtgtggcctg gctggtcaag acccatatga aatttcactt
      721 ttttgccaat acggctgaag gggacgtcga caagtggctt cgccgcgaag ccctgcaagg
      781 gccttttcac cggacagaag aactggctga agccatccgc caggcgacgg cgctgtccgt
      841 cggcgatatc atcggctgcg gccggcctga ctctaatacg gaagggacag aatcctttgg
      901 cgagtatatg gccatgctgg cagaggccat gccggtcagt acgaaggatc tcgtctatga
      961 ccgccgcatt cctgatctgt gtcggaaccg gaccggtcag tgcctgcgca tcctgttgaa
     1021 acgggttcag aataaggatc tcgtcaacga tgccgatgcc ttggcggcgg cagcccggcg
     1081 ctggatggaa cgccatgaag gggatgca
//
LOCUS       DAWDXZ010000513         1104 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_34438_length_1104_cov_8.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000513 DAWDXZ010000000
VERSION     DAWDXZ010000513.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_34438_length_1104_cov_8.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          239 a          301 c          274 g          290 t
ORIGIN      
        1 catcgtatcg atccatcaga atgaaaatga tttcgtaaaa tatgccgtca ttcgtcccgg
       61 cgtcgatttc tcgaaattag aagaagtctt cgtcatcttg aaatcggccg ataccggctc
      121 ttatgaaaaa ccggacgtca cgccgaaact cgttccgcag acgaatcgcg acaaggtgga
      181 aggcattaaa ggagccgctg cgttatgaag aaattgaact gcatcatcac agccttcatc
      241 gtctttatcc tgcaggctgc ggtcttgcct tttatcttta acggtgtaag ccagcccaat
      301 ctgatcttcg tcttcgtcgt cctcatgggc ctccatcacg gccagcgcgt cggcattgca
      361 acggctctct tgggtggttt ttgccaggac gtcatcatcg gcaatttctt tggcctgcac
      421 ctcttggtgt atctcattgt cacccttgtt tgcagctatc tcggccgcaa catcgataag
      481 gatcagtgga tcttgaccct gctcatcgtc ctcggggcga cggaagccgg tctcatcctg
      541 acctgcggct tcctgtttct ggccggccag tacatcaatg tggcggcgta cttattggaa
      601 ttcagtatac ccgtactcgt ttatcacgga atcttggcct tgcccgtgga ccatgtcgtc
      661 tggcgcctgc gccggtccga caccttttat ggctttccgg gctatcgctg gtaactaaag
      721 gagacacaga tacatgcttg aagcgttatt aaaaaagaaa aatcaagacg gccgcttccg
      781 gtatctttac tgggtggtca tcgcgatttt tgtgattctc gtcagccggc ttatctattt
      841 acagcttttt gccggtgaat attatcattc cctggccgaa gggaaccgcc tgcgggccat
      901 ccccattgcc gccatgcgcg gcatcatgta tgaccgcaac ggccagattc tggtcggttc
      961 ccggcccagt ttcatggcga cctatgtacc gccgaaaggc ggcatgagcg acgaggaact
     1021 ggccaagtta gccggtatcc tcaacgtacc ggaagacaag ctgcgcgata agattcaaaa
     1081 ggtaaagagt tcttacgtgc cgac
//
LOCUS       DAWDXZ010000514         1100 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_45866_length_1100_cov_9.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000514 DAWDXZ010000000
VERSION     DAWDXZ010000514.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_45866_length_1100_cov_9.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          274 a          293 c          303 g          230 t
ORIGIN      
        1 atctcagcct gtccgagaaa aaagtcctca ccttagattt tcccatgacc cgtaaggatg
       61 aagtcctggc cggggcccat gaaaaagcgg ctcaatcgat tcgatctgaa ctcgatgaag
      121 gccgggacgt ggccctcatt accctcggct gcccgacggt ctatgcgacc agcatttacg
      181 ttcaccgcct cgttcgggac gccggctatg aaacggccat cgtccccggt gtgcccagct
      241 tctgtgccgt ggccgctaaa ttgcagcagc cgttgtgcga aaaagacgag gccttgctga
      301 tcattcccgg caaccataaa aaccgccggc agctgctggc tctcccgggc agtaaggtca
      361 tcatgaaacc gtcgggaaaa ttgggaccca tccaggatga actgaaagaa ttgggcctcc
      421 tcgagcggac ggcgatgatc gagcgctgcg gactttccgg cgagaaaata tatgaacagg
      481 ctgccgatgc agaagagacc gggtatttcg ccgtcttatt ggtgaaataa tcggtcttag
      541 aaggacgttt cggcagccat ttacaaggag gagtgacacg atgaaaacga tcgttggcaa
      601 tgtcatgccc gaagaaacgc gcatggctat cctcgaagat ggtcgtcttc gcgatttcgc
      661 ggtggaacgc gacgatgaca tgcatatcgt caatcatatc tataaaggca caatccaaaa
      721 tattctgccg tcccttcagg ctgccttcgt caatatcggc cgccgtaaaa atgcttttat
      781 ctacatgggg gatatgtttc ctcgagcggc gacgaaagaa gaaatccagc agatgcatct
      841 gtctgtcggc cagtcggtct tagtccaggt catcaaggac gaacagggct cgaaaggacc
      901 gaaggcgacg gccaatgtca gtttggccgg ccgctatgct gtattgatgc cgaccgtcga
      961 ctacatcggc gtatcgaaaa aaatccgcga tgaacaggag cgggatcgcc tgcgcgacat
     1021 cgtgtctgaa ataaagccgg acggcatggg ccttatcatt cgcaccgtcg ccaagggcgt
     1081 atcacgggaa gaactcttag
//
LOCUS       DAWDXZ010000515         1093 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_11372_length_1093_cov_7.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000515 DAWDXZ010000000
VERSION     DAWDXZ010000515.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_11372_length_1093_cov_7.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          259 a          278 c          273 g          283 t
ORIGIN      
        1 cgagttcgct aatccgttcc acataatgac gggctgcttc cggcagttca tcataggatt
       61 tgcagtttga aatcgacgtc ttccaacccg gcatatcttc ataaatcggt tcgacgtttt
      121 caaggacatc gaggctggcc ggatattctt tcagctgctg gcccttgtat ttgtatcccg
      181 tgcagatttt aatcgtatcc aagtcgtcga ggatgtcgag gcgggtaata gccaaataat
      241 cgaggctgtt caacatagct gcgtatttta caacggccgt atcgagccat ccgcagcggc
      301 ggggacgacc ggtaacggta ccgaattcgc gacccgtatt gcgcagataa tcaccgattt
      361 cattgagctg ttccgccggg aacggaccgg caccgacacg cgtcgtatag gcttttacga
      421 caccgacgat attttccatg cggcgaggac cgacaccgga accggtgcag gcaccgccgg
      481 ccgtcggatt cgagctggtg acgaagggat atgtaccatg gtctaaatca agcatagctg
      541 cctgagcccc ttcaaagagg accttcttac cttcactgac gtagaggtta gccgagtagt
      601 tcgtatcctt tacatactgg cggatttggt cggcataacc gaggtattcc tggagtactt
      661 catcgtaatc gaagccctgg cagccgtaat atttttcaaa gagacgattt ttcaattcca
      721 tattgctacg caatttttcc gcaaaaacat cgcggtccat gagatcgcca atgcgaatgc
      781 cgacacggtt gattttatcg gcatagcagg ggccgatacc atttttcgtc gtgccgattt
      841 tagcatcgcc cttgagcttt tcatcttctt catccatacg gacatggtac ggcataatga
      901 cgtgtgcccg cgtagaaatt tccagcttac tgatagttac gccttttgaa gtcatgcttt
      961 caatttcttc gaggaggact ttcgggttaa caacgacacc gctgccgatg atacaagttt
     1021 tgtcggaaaa gaggattccc gacggcaaga ggcgcagtgc aaaggactta tcgtcaacga
     1081 cgaccgtatg gcc
//
LOCUS       DAWDXZ010000516         1090 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_2423_length_1090_cov_9.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000516 DAWDXZ010000000
VERSION     DAWDXZ010000516.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_2423_length_1090_cov_9.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          227 a          245 c          250 g          368 t
ORIGIN      
        1 aagaaggcct gaggatgagc acataccgga cagatcttcg gagcttcatc agcttctacg
       61 atgaaaccac agttgcggca tatccacgtt ttcttttcag aacgtttgaa tacctgtttt
      121 gattcgatgt tgttgaggag cgctaagtaa cgttcttcgt gatgtttttc gatttgagcg
      181 actttttcga acaagaatgc aatcttgtcg aagccttctt cacgagcttc ttttgcgaag
      241 gtcggataca tttccgtcca ttcgtaatgt tcgccggcag ccgcatcttt caatgcatcg
      301 acgtcgttcg gcatttcgcc gccatggaga agtttgaacc agattttagc atgttctttt
      361 tcgttgttgg ccgtttcttc aaagaaggct ttgatctgtt cgtagcctgc tttttttgct
      421 acgctggcat aataggtgta cttattgcgt gcctgggatt cgccggaaaa cgcttcctgt
      481 aagttttttt ctgtttttga accttttaat tccataactc aattcctcct taatagtaat
      541 tcattcatcg ggacgttgtt atttttagca cccctccggg ttgatattat tgtaacacct
      601 ttcgatttta ctgtcaacga agattcattc cttgttgata aaaaggaata gggatgaatt
      661 actgatcttt ttcgtctgcc gcgcgattat tatcgtgaat gacggaacga tcggatgatt
      721 cggaccggga tcttgtatcg tcgcggcggg tatcttttgc cggttcttta cggcgaaccg
      781 acgaatcaga tgacttttcg gaatcggaag actcatcgtg gctgcggtcg ctgtcgctgc
      841 cgtcatgaat gcgggcatcc cattcatttt ggaggtcgct gccggcttct tcggctgaat
      901 ctttcaggtt gccgtacttc gattcaaatt ggcgttcgat ttcggcggcc ttttcaggat
      961 acatggtccg cagtttatcg agcatttcct ctttgctggc attgccctta tctaaagcac
     1021 cttggagttc tttatattcc ttataataag tcatccaacg cttcagggcc gggtagtcgc
     1081 tgctcataaa
//
LOCUS       DAWDXZ010000517         1084 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_19204_length_1084_cov_11.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000517 DAWDXZ010000000
VERSION     DAWDXZ010000517.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_19204_length_1084_cov_11.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          273 a          312 c          287 g          212 t
ORIGIN      
        1 ccgctcgaaa ggtgatttag gcgacaagcc gatcgatgac gtcatcgaac acatccttcg
       61 tgtgagcgca actagagaag gctaatctcc tttgtcgaaa gcatgcccta tggtaagccc
      121 tcgaaggacg aaatcgctta tactgtcatt cgctgtctca ggacagtgaa aaggggctgt
      181 ttttaggaac agcccccttt cttttttcta taaaggagtt tctatgataa tcaactggta
      241 tcccggccat atggccaagg ccaagcgcct gattcaggaa aatctcaaaa tcatcgacgt
      301 cgtcatcgaa ctcgtcgacg cccgcatccc cttgagcagc accaacccca tgatcaaatc
      361 cctcatcggc gacaagccgt ccgtcgtcgt cctcaataag gccgatttgg ccgatccggc
      421 cgtcgtcgac gaatgggtgg cctactataa acagcagggg agaaaggtca tggccctcaa
      481 tagcaaaggc ggcaagggag tcaaacagct cgtgtccctc atccgctcct tagcggcgcc
      541 caagctcgaa cgctggaagg cacggggcct caagaaccgc gccgtccgga ccatgatcct
      601 gggtatcccc aacgtcggca agtcgacgct gatcaataag ttgtcccaca gaagcgccgc
      661 caagacggcc gataagcccg gggaaacgaa gggcaagcag tgggtccaca tcggcgacca
      721 gctcgacctc ctcgatacgc cgggcgtctt gtggccgaaa cttgaaaatc agaccagtgc
      781 ctaccgattg gccgcaacgg gggccatttc cgacacggtc ttcgatatgg aaacggtcat
      841 ttgccggctc atcgaggagc tgaccgaccg ctatccggaa gccctgaaag gccgctataa
      901 gctggaagcc ctcagcgaca aacctttaga aacgattgaa gccatcggcc gcaagcgggg
      961 ctgtatcgtc tcaggcggcg tcgtcgactt agaaaagaca tataaactca ttttgaagga
     1021 attccgcgaa ggcgtaatcg gcgccatttc cctcgaccgc ctcggtgaat ttgaagggga
     1081 tgaa
//
LOCUS       DAWDXZ010000518         1069 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_8405_length_1069_cov_12.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000518 DAWDXZ010000000
VERSION     DAWDXZ010000518.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_8405_length_1069_cov_12.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          260 a          283 c          267 g          259 t
ORIGIN      
        1 taagacctca ccggcttccc gggtatagcc gccataaggg taacagaagt attcattggt
       61 aataccgagc tgttctttta actcagcctg actcttttta atctcgtcgg caaaggtgtt
      121 ccccgtcatc tccgttgccc gttcatgacg atagctgtgc gacgcaatgg taaggcctga
      181 agcctgcatt tctttgacct gctgccaatt gatgcgtgtc ccctggtccg tgtcataagt
      241 cggcacgaaa accgtcccgg caaaaccgta ttcctttaaa ataggcatga cgatacggta
      301 cgtatcggga tagccgtcat caaaggtaag gacaacaggc cgtaccggca ccggtttgcc
      361 atgggccaag tagtcgtaaa gctgatccat ggtcagggga tgaaaatcgt tatctttcaa
      421 aaatttcatc tgctcccgaa agtgggactc caacagaacg gcgtcattat ccggaacgtc
      481 gccgatcata tggtacatca gaacgggaat cccctctttt ggtacagcca tagcaacttc
      541 ctgcgacggt ttcttttccg ccgcaggtgc atcgaccttt ttattgtcca gtgcgcagcc
      601 tgccagtata aggcagctca acagagccat acaggctaat ttcaagatgc gcaaatcgta
      661 cagcccctct catcaagatc attcattgtc ccgcaaggtt actacgggca attcatcata
      721 gtggccggcc aggaacagcc ggatataacg ttcagcatcg tcgcggatat cacgcatcgc
      781 ttcaacggaa tggaagctga cgctcggcgt caataaaaat tccggccggg caatcatttt
      841 gagcagcatc gggctcggat gctctgtcga cagggcatcg agggcggcac tgcggatatg
      901 gccgtcatcg acggcatggt acagggccgc ttcatcaact aagccgccgc ggcagcagtt
      961 gacgaacatg gcaccttttt tcatagcttc gaattgttca tcttgaaaaa tgtagcgtgt
     1021 cgattcgttc agcggcatgt gcagtgatac gacgtcgcta gtctgaaga
//
LOCUS       DAWDXZ010000519         1059 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_41370_length_1059_cov_14.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000519 DAWDXZ010000000
VERSION     DAWDXZ010000519.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_41370_length_1059_cov_14.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          321 a          209 c          285 g          244 t
ORIGIN      
        1 atgaatataa gagcagttca gccgcacgcg atgccgcgtg gaacgagttt taccggagaa
       61 attacggtcg catcgagcaa tatgccatgg aagtcctcag ccaatatggg gccgataaaa
      121 ttgccccctt cttcgatggg tcggaacaag ggatgtatgc caagtggaac aaacagttcg
      181 aggccatttt gcaggacacg gcagaccgcg tagtcgacgc cttgggagat tgtgctttgc
      241 cggaagattt ttgcacctgg aacgtcttgc cgacgcaata tatcgatagc ttcaaagact
      301 ataatcccat caaatggggc gccaaggtcg accggaaaat gagacaggac ccctatgcca
      361 atgaagcctg gtataaacgg tgtatcgata ccgacgagat ggaacgagtg gaagccggcc
      421 ggatctttaa aggcgtgctg cacaacgtaa cccggtattg ctatgactac gatatcggta
      481 tgggaggcga ttttaaaaag gatatcctgg acaccattta cctgatggat atggatgaat
      541 attacgaaga agtcctgggc tggatcatcg acgattatcg aaggaacgtt gagaaaatga
      601 ttgcctcgaa atgtgaggcc ttggaagggg ccctcggata agaggcgggt taacgtacaa
      661 aaaaatggac gagagtgaga aatagtcgct tgcgtccatt ttttatggtt ggaaaaatta
      721 ggattgcagc cttaagggcg ttatggacgc ggcgggacga tattatggtt ttaagtaaaa
      781 tggtgctata atgaaattaa aagtaacggg aaataaccat tgagccgggg gatgctcgga
      841 gcataggaaa tcatataaat ctatggcgga aagcatgccg tgaaaacgta cgcagtgcca
      901 aggtatagtg aacatccttt tagccccgga aaaaccttta tgaaagccgt aacttttcat
      961 tgaatcccta aatttaggaa gaaatgcccc gtatctgtag gagcctatcc cataggtaca
     1021 aacaaaagtg gttgtaagaa aacacttttg ctgtcgcac
//
LOCUS       DAWDXZ010000520         1056 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_3635_length_1056_cov_11.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000520 DAWDXZ010000000
VERSION     DAWDXZ010000520.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_3635_length_1056_cov_11.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          305 a          261 c          215 g          275 t
ORIGIN      
        1 atcagctccg gtaaaagctt ctttggcatc aagagtcgcc ttaaagtgca ggtcgtcacg
       61 ctgcaaaaaa gctgaaatat cagcatccac aatgggagac tgacctttat ttaacaggga
      121 caccttttct ggaataatgt ccaaagcaat aacttcatta tgctgagcca gcagcatact
      181 attcgaaaga ccgacatatc cggtcccggc aacagcaatt ctcatatgta ttccctactt
      241 tctcttcttc cgagcacggc aaattcgagc tccgtcgtgc caccgataaa aagcatacca
      301 aaaaggcccc gcaaaaagga tggatcaacg gactgcgggg cctcgtttat tataacttat
      361 agcgaatttt cagccagcta taccaaaaca ggcattgaat tcgtttccac gtttcgccct
      421 tttcctgata gacttctgcc agccgtttga agctgtggta gtaggacggt tccctaggag
      481 gcatgggatc gttcatgctg tcccagaagg aaatatctag gtagtggcgc cacaattcat
      541 catccgaatc aaggactaat tcccaaggct tgtcccgacc ggaataatga atcaggattc
      601 cttctatgct gaaatcttta ttcttgtgat ggaataaatt cggaatgggc ttcatattcc
      661 cgtcgatgac aagattcaac aaatcctgag tctgtccctt gaaacgccgc ggatcggccc
      721 cttgataggc aaagacctgc tccgtaattt tttcacgaat ccattccttg acatcgatcc
      781 acatcaagcc gtctgaaaag tagacgtcct gcttcatctt caaaaaggcc acccggtccg
      841 gtgtatgata ggtcaaggca ccgatagctt tgccttctaa atccgtcgtc aaaaactgtt
      901 ttaaactgcc aacacatatc atatctgaat ctaagtacaa gtagtggtcg gtgtagttcc
      961 gtaaaatcca gggcataacg atgcgaatat acgtaacccg agaaaaacga gcgactttga
     1021 tatgaaaatt ctgatagatg ctcatatcca tcacat
//
LOCUS       DAWDXZ010000521         1048 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_34408_length_1048_cov_13.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000521 DAWDXZ010000000
VERSION     DAWDXZ010000521.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_34408_length_1048_cov_13.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          225 a          253 c          319 g          251 t
ORIGIN      
        1 aaagcaggag caggaaaatg aagctctgcg ggcacaggtt gaaaaacagg ctcaggaaaa
       61 tgccgaatta cgcacacaag ttcaggacat cctgcgccag ttggcgaaca aataggccga
      121 aggttaacac gtaatcaaca tcctttagca caagacaaaa cggggctgtc ccgaggtcat
      181 cataccttgg agcagtcccg tttttctatg gatgcggaat tggcagctag acgctcattc
      241 ctttggcgta gttttcgtac aattctttat agtagccgcc gcgggccaag agttcaccat
      301 ggggcccttg ttcgacgatg cggccttggt tgacgacgag gaggaaatcg gcgtcgcgaa
      361 tggtcgacag gcggtgggcg atgacgatgc ccgtacgccc tttcagcagc tgattcatgg
      421 ccttttgcac ttctacttcg gtgcgggtgt cgacgttggc cgtcgcttca tcgaggacca
      481 agatgctggg attgactaag aaggcgcggg caatggtcaa taattgccgc tggccttgag
      541 acaagtcgtc ggcaccgttt ttcagcaccg tgtcgtagcc gagggggagg gtacggatga
      601 aatggtcggc catggccagt ttggcgacct tgatgatttc ttcgcgggag gcgtccggtt
      661 tgccgtaggc aatgttttcg gcaatggtgc ctgtgaagat ccacgtatcc tgcaggacca
      721 tgccaatctg tcggtgcagg gtttcacggc gaaggtcacg gatatcggtg ccgtgaatgg
      781 taatggcgcc gccggagatt tcgtaaaagc gcatgaggag attgatgatc gtcgtttttc
      841 cggcacctgt cgggccgacg atggcgacga cgtggccttt gggaatctca aaattcacat
      901 cttccatgag gggcttgtcg gcgctgtagc cgaaggtgac gtgtttaaat tcaatggcgt
      961 tatcctttga ggccgtcgcc ggttgtcgcc gtgcttcatc ataagacggt tcttgccgac
     1021 ctgtcttttc atcaggcagg acctcgat
//
LOCUS       DAWDXZ010000522         1047 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_13347_length_1047_cov_12.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000522 DAWDXZ010000000
VERSION     DAWDXZ010000522.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_13347_length_1047_cov_12.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          226 a          285 c          274 g          262 t
ORIGIN      
        1 gaccatgtcg cccatctttt ggccgccttt atagacacta aagtgaagtt tgcctttcag
       61 gtaatgaagc tctcttgcct ggtaatccgc cggctgttcg aggccgtaag aaacgtagcg
      121 ccgcccgatg gaaggagcca tggcgcgaat cttgtcgttt tcaaagcaca agacggccag
      181 tcccttttcg ggatcgagtt tctggatgaa ttccttaaaa gcggcgatga cgttttcaag
      241 ggagttatag tgatccatat ggtcggcatc aatgttggta acgacggcga tatacggatg
      301 ctgcttgagg aaggtgccgt cgctttcatc ggcttcggca atgacgtact gccccttccc
      361 taagatcgag ttgctgtgca aatagtcgac ttccccgccg atgacgatgg tcgggtcctg
      421 tcggcattct tcaaagacct ggcccagcat ggacgacgtc gtcgtcttgc cgtgcgcacc
      481 ggcaacagaa atcccctttc cccaactcat gatgtgaacc agcgcatcgg aacggtggat
      541 gaccggaatc tgccgacgcc gtgcttcgac gagttcgacg ttgttgtcgc ggatggccga
      601 cgaaatgacg acgacctggg cgtcctgcaa attttcaggg ctttgtccaa tgcagatacg
      661 ggctccgcgc ttggcaaact gctccgttac aggcgaggct ttgaggtctg agccggatac
      721 gtgatatcct ttttcaataa aaacattggc gattgcccgc attccggctc cggcaatccc
      781 aataaaatga acgtttgtca catcgttcag attaaccata ggtttattcc acacccttct
      841 tctcctgtta cggacgacgg tttcgggcaa tcgacaaggc cagttcagca atatcctgag
      901 ctgcctgggg cttacccatc gaacggctgg ctttgcccat ggctgccagg gccgacggat
      961 cgtcttttaa atgaatgatt tcttccaata gttcccgccc ggtcagcatc ttatcgagaa
     1021 tcatcttggc ggcgccgcac atgacca
//
LOCUS       DAWDXZ010000523         1039 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_5224_length_1039_cov_7.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000523 DAWDXZ010000000
VERSION     DAWDXZ010000523.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_5224_length_1039_cov_7.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          264 a          263 c          227 g          285 t
ORIGIN      
        1 actcggcata aacaaatcca aagatttatt tactggcgtt cattcaatca ttgttcagtt
       61 ttcaaaggtc atcgtgtcgc cgtagcgacg acttatttat tttaccaaac tacgaagagc
      121 ttgtcaagca tgtttttgaa ttttttcata aaacctgaca accataaaca ggcacaacaa
      181 aaaaaactta cattttcttt ctcacgtctc gttgcagctg gtttttatat aataacatta
      241 acgccgagaa aagtcaacta cttttctcgg cgtctttctt aatgttcttc ccccatgacg
      301 tcaaggccta ccatagccat cgctttggct ccggtcaggg cctgcatttt tcccaaatca
      361 ctgccggccg ccgctgcaaa ttccggcgta tgaccggaca taccgtaacc gatacagatt
      421 tccggttggc tgaccctcgt cacttgactg acgttgccca catcggtaga accttcggca
      481 aagtcgtcac tgcgaaggcc gaattcttca tagagtcgat taaaggcatc ggcaaagtat
      541 acatccattt tgggatcgtt gaccatattt ttatagagcg gttcatagtg aaccatctct
      601 accgaggctt gatgagccgc tgcgacatcc tgcgccatct tcttgatatc cggcagcatt
      661 ttatgttcca aataatcgac agtcaaggcc cgaatcgtcg ctttcagaac ggctcggtcg
      721 ggtacgatat tcggcgccgt cccaccttca gtgataatgg cgccgatgat atggcgctca
      781 gtcagcgtct tttcgtaggc cttgaggcgg ccatagaaat caactaaggc atcgagggca
      841 ttgatcccgt gatattctgt atcagcgaca tgggcggctt ttcccaaaaa agtaaattgt
      901 aagggatggg tcgcatagga cgtaccgcca atataggttt tatcaacgcc gggatggacg
      961 atgacggcag cgtgaaggtc gtcaaagaca ccgtgttcac tcatatagac tttgctgccc
     1021 accgtttctt cagcaggac
//
LOCUS       DAWDXZ010000524         1036 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_12001_length_1036_cov_14.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000524 DAWDXZ010000000
VERSION     DAWDXZ010000524.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_12001_length_1036_cov_14.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          258 a          243 c          219 g          316 t
ORIGIN      
        1 tggaagagct gggaagcctg ggtccgaatg accggttcaa agcgaccttc ttccttatca
       61 cgaatatagc ggatgctgcc ttcgatgcag tccagtttca gctgactttc cgggttctta
      121 tctttttgac tccccttttt attgccattg gatcggagat agcggccggg gataaattcc
      181 ttcagcaaat cgtggaataa attgattctg caggactggc cggataactt atagaagcag
      241 tcgtactgtt ccttgaccag atcatctacc ggcaggattc gcttccaaag tgcataaata
      301 tcggcataga ggatgcattc gatttcctta atcgtaattt taatcccttc aaggggattg
      361 agggtctttt ttaattcgtc cgccgcatcc tcccagatgt acagataata ctgtgcttca
      421 tccccgatgc ccagtgccct atctttgtca tcactaaaat tgatggaaac tacgtctcgc
      481 tgagtctgat aaaaggcttt cttatatgca tcggccatct tccacaagta gtagaagttg
      541 cgcttcatct ggcgcttttc attttggaat aaaccatctt tttcaaagca ggtaggaatg
      601 atttcttcag catccttata ggctgcttcg aatttgctat aaatcttttc gcagccagtt
      661 atcaccgaat cggaccggtc caatatgtcg agaatttcat tttctccggc aatcaacttt
      721 agcatcgata tatcgtcctt acatgccact ttgaaagcca gctttatctt aagcatttga
      781 aggatgcgga aggtaatatt attgccgcca aaattggagt caccattttc cgaactcatc
      841 gtaatatcaa gctggcgatg accttcttga tttttagaaa tctgatacgt gcagcgagcc
      901 aaatcggtcg taccgccgcc gcagtcgata atcaagatgt tctggggtgg aaaagccttt
      961 gcctgtacca aatccttacg ggagcgttcc atttctttcg caatatgttg gtagacgacg
     1021 gcaatgcctt catcca
//
LOCUS       DAWDXZ010000525         1029 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_14504_length_1029_cov_10.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000525 DAWDXZ010000000
VERSION     DAWDXZ010000525.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_14504_length_1029_cov_10.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          293 a          251 c          211 g          274 t
ORIGIN      
        1 atatcatcgg taaagaacat tgcggctaca agcgggcctg caattcccaa cgacaggaca
       61 gttaccgtaa taaaggttgc ggctgacagg gaaccattca tgtaaaacag gcacccgacg
      121 ggaagtacgc taatcaacgc cgcgggccat acactcatca taatcgccga atatttttgt
      181 gtatctttca tccagtctaa aatcaggtct gcgtttgcat ggacggcatc tgtaaatttc
      241 tgataggaat tatctgcctg attaaacgcc ttaatgactt caatgccgcc gatgtattct
      301 accgttgttg cgctcatgtg atttccagct tttacaactt ccccatactt cttgggatat
      361 tccttcatca ttcccatgta gcacatcatt ccaacaggaa tggtaatcag cgatactaac
      421 gccattcgcc agtccagaac gaacaaataa acgatgattg cgattggtac gagaagattt
      481 gaagtcattt ccgggattac atgagcaagg ggaacctcta actgttcaat tcgttccacc
      541 atgccatttt ttagttgccc ggacggagta ttgagaatat agcccatagg cactctggta
      601 agttttgagg caattttccg ccttgcctcg gacagcactt caaaggtcgc ttcatgggat
      661 gccctcgtgg aaattcccat caatattgct tttaggacaa agcagccgcc ggaaatcaga
      721 caccacacca tgtaaaatga taaatcccga ttcccggaga gtaactcaat cgtcattctg
      781 gctgttgcaa aataggggac gatgccaaag gcaactccca gcacagcaag aacaacagaa
      841 atgatgtagc cagtcttatg cggcctggca aactcaataa gtctgccaaa tggatttccg
      901 ccttgcgact gatccatata aatccctcct ttagttagat tttgctaact cttattcaaa
      961 agaataagcc gataatctgt catgtgattt acacaaacaa attatcggct ctgcgtcttt
     1021 gcgtctggc
//
LOCUS       DAWDXZ010000526         1021 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_37541_length_1021_cov_9.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000526 DAWDXZ010000000
VERSION     DAWDXZ010000526.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_37541_length_1021_cov_9.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          266 a          236 c          203 g          316 t
ORIGIN      
        1 acacgccgcc gggctggaaa atagtgcttt ttccaaaatc gtctggaata actgaccact
       61 gccacgtttc ttatcaatat ccatgtcgag tttcatgtta ctcaacacat catacgcatg
      121 ttcctctgct tgtgaggctt ggcacgtctc ttcggtaata gttcgttcca gtacagcctt
      181 gttcatttgg tctttgacat ctttcttgaa acgacggata cataatccct gaatatcctc
      241 tttcgtatat ttcttaggat tggcaatcgc tgttgggtcc aacatattca tgagagaagc
      301 aaagctttcc gatcggccat catgcggcgt tgccgaaagc attatcatgg tatccgaccg
      361 tcctgccaat aattctgcta atttagcccg ctgcgctctt cgatcacctc gagtcgccac
      421 attctgtgct tcatcaatga caatgatatc ccaataggct tgttccaaat gcatacgata
      481 ggcgacatca ttttttaacg tatcgataga aatgattgtc ttgtcatagt aaaaaaaggg
      541 gttatagttc acaggtaatt tagcccggat ttgatgaatc ttattagagt ccaagcgtac
      601 cagcggaatc gtgaagcgat tccacatttc tttttgaaac tgcgtcatca tggatttacc
      661 tgttacaact aaaatccgtt tgcccttgcc gcgcacaatg agttcactta ttaaaatacc
      721 tgcttctaat gttttgccca aaccaacgtc atcagcaatt aagattcgct gtcttggttg
      781 ctttaaggca gtgactgctg gaagtaattg atacggaagt ggatccatcg ctgctcggtc
      841 ccctatatgg atagctgtat ccgtcgggat cttctgtcgc cattgacttt ccagatacaa
      901 caaggtttcc cgataatgcg gagacatatc gataaccagc tttacctttg ccggatcaac
      961 agggatgata gtatcaatat cttcgagaaa aatagcttct ttatctttta ctaaagggga
     1021 a
//
LOCUS       DAWDXZ010000527         1018 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_35839_length_1018_cov_12.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000527 DAWDXZ010000000
VERSION     DAWDXZ010000527.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_35839_length_1018_cov_12.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          281 a          255 c          243 g          239 t
ORIGIN      
        1 aatcgtaaaa gaggccgtaa ggtctctttt ttattatgcc taaaaccgat agccttcttt
       61 cttctcttgc aaaaaaagtg tagcaaccaa atagattttt aggcttgtcc tattgcaaat
      121 ccgcatactc atgttatact ttaggtatac aaaaactttt agattgtcta aaatattttt
      181 ttcagtttat tttaggaggt acggattatg aaaaaggata tgaaatgggc catgaatcat
      241 ttgcccaaat cagatgataa aaatctcccc atcatgagcc tcagtgaaat cgaaaaggcc
      301 cgagctttcc atcagagctt ccctcagtac agcgaaacac cgcttacgcg cctcgatcag
      361 atggctcact acttaggctt gggttcgatt tttgtcaaag acgaatcgta tcgtttcggc
      421 ctcaacgcct ttaaagtcct aggcggctcc tactccatgg ccaagtacat cgccaaagaa
      481 acgaatcgcc ggattgaaga catgccttac caggtgctga cgtctgatga gcttcgaaag
      541 gatttcggac aggcgacctt ctttacggca accgacggca atcacggccg cggcgtcgcc
      601 tgggcggcac aaaagctggg acaaaaagcg gtcgttcaca tgcccaaggg tacgacccaa
      661 acccgtttgg aaaacatcaa aaagctgggt gctgacgtcg atatcttaga actcaactac
      721 gacgactgtg tccgtcaagc cgccaaggaa gccgcggaaa cgccgcacgg cgtcatggtt
      781 caggatacag cctgggacgg ctacgaagaa atcccgtcgt ggatcatgca gggctatggg
      841 acaatggcca tggaagcggg cgaacagttg aagaaagccg gcgttgaaag accgacgcat
      901 atcttcgttc aagccggggt cggctctctg gccggcgcca tcgtcggata ttttgccaat
      961 ctctataaag acaatccgcc gaaattcatc gtcgtagaag cctcggccgc agactgcc
//
LOCUS       DAWDXZ010000528         1008 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Megasphaera massiliensis isolate gSpreadC_ID_3164
            k119_20729_length_1008_cov_14.0000, whole genome shotgun sequence.
ACCESSION   DAWDXZ010000528 DAWDXZ010000000
VERSION     DAWDXZ010000528.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996621
            Sequence Read Archive: SRR8800859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Megasphaera massiliensis (human gut metagenome)
  ORGANISM  Megasphaera massiliensis
            Bacteria; Bacillati; Bacillota; Negativicutes; Veillonellales;
            Veillonellaceae; Megasphaera.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Megasphaera massiliensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k119_20729_length_1008_cov_14.0000"
                     /isolate="gSpreadC_ID_3164"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1232428"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          228 a          262 c          253 g          265 t
ORIGIN      
        1 cggtcttacg ctgccccaag tcgcggacca tgtaggactg gcggttcagg ttgctggcat
       61 cgaccgtatc gaagtcgatg aggtgcaggt gcccgacgcc ggtccgtgcc aaatagaccg
      121 ccgcattaga accgagtccg ccgagaccgc agatggcaac ccggccggca ctgacctttt
      181 catggacttt cggcgtgtgg cgggaacaca tcatggcctc caaggcttct ttcggcggca
      241 gccggtcttt agggataaaa tagatttcat cgccgtcttg taagggtaaa ttttctgtcg
      301 tcgcaaaacc gttgacgatg gtgatttcct ggcccttacc gtgggcatgt ttcaattctt
      361 ctaatgaaga ccaagggccg tcgacgcggg ccccattcaa taataattgc atcgtatcca
      421 cctctcgtga gcaaaataaa aacgcacgac gattgacacc ttgcgtggtg tccccgtcgt
      481 gcgtttgcgt tctatgactg tagtgtactc gtttttatcg tacgtgtcaa tctttctttt
      541 cagccttatc ggcctgtgcg ccctctttca gccgtttcag ttcgcgctgt tcttccggcg
      601 gaagggacac gccgcagcat ccgcctgtcg aattgctggt atagcctttc ataacttcgc
      661 tgacgacatt ttttaatttt ttaacggaat cgactaccat aataagcccc cctcaacgtc
      721 tgtttgacgt tacaaatgca aggaaataag aacgtctatt tatacttatt atatcaagaa
      781 aggcttacgg tgtaaatcat attagccaat actcattact tcttatagta aaaatccgga
      841 aaaggcggat tatcgtccgt caggccatac gagagtgatc tcgccggact cacgcgtctt
      901 ctgaaggtcg atgatgcgct ggttcgacga gcctctgaaa tagagtcccg ggtctttcaa
      961 agcgtcgacg aaggggccgt cgacgaggac gtcgcactga tcgaggag
//
